LOCUS       DASZMC010000001         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1923614,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000001 DASZMC010000000
VERSION     DASZMC010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923614"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VG274_00005"
     CDS             <1..745
                     /locus_tag="VG274_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWE05057.1"
                     /translation="SDLDWIARGAALFDMRVRAVEEGGLAVVGNYADKIFEAAGFGDA
                     LLPLTFRKMFWRGLDVTLSHFGEHGGFEIWCKADDAPIVWDRIAKAGAPFALTLAGLS
                     AMDILDLESGVLRPERDYEAAREDFVDTPTPFELGLESLVETDHTIFNGRAAYLAAPR
                     VRTRVGVELDVETPAPRTPLRRNGQLVGETMSSLYSPALQRAIALAVVKVSDSEQGTV
                     LVLPDGRSARVCALPFLPVPSDGNGTAPR"
     gene            812..1354
                     /locus_tag="VG274_00010"
     CDS             812..1354
                     /locus_tag="VG274_00010"
                     /inference="COORDINATES: protein motif:HMM:NF004113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWE05058.1"
                     /translation="MIDPDTDLVARAGQGDRAAAQALMARHLPKMLTVARRMLSSQAE
                     AEDAVQDAFLKVWTHASRWQPGKALFETWLYRVVMNKCYDRLRKRPNASLDEAVEVPD
                     VAPQADAVMENTALGKEIEVALAALPERQRAAIVLCHHQECGNIEAAEILGISVEALE
                     SLLSRGRRTLRARLAHLKDG"
     gene            1367..1714
                     /locus_tag="VG274_00015"
     CDS             1367..1714
                     /locus_tag="VG274_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05059.1"
                     /translation="MENLPNEDAIWVTDALKALPTVPVSAALQQNILASFEAVTARRN
                     AGFAGAVRRLAATVWPGVPAWRPATALAVSLVIGLAAGTLVPLEGAMADNNEQTASVV
                     LDAPPSFDLGENS"
BASE COUNT          310 a          535 c          591 g          332 t
ORIGIN      
        1 atccgatctc gactggatcg cgcgcggcgc ggcgctgttc gacatgcggg tgcgcgcggt
       61 cgaggagggt gggctcgcgg ttgtcggtaa ctatgcggac aaaatcttcg aagcggcagg
      121 gtttggcgac gcgctcttgc cgctcacgtt ccgcaagatg ttctggcgcg gactcgacgt
      181 cacgctgtct catttcggcg agcacggcgg attcgaaatc tggtgcaaag ccgatgacgc
      241 gccgatcgtc tgggaccgga tcgcgaaagc gggcgcgcca ttcgcgctga cactggcggg
      301 tctgagcgcg atggacatcc ttgatcttga atctggcgtg ctccgtccag agcgggacta
      361 tgaggctgcg cgcgaggatt ttgtcgacac accaacacct ttcgagcttg ggctggaatc
      421 gctagtcgaa accgatcaca cgattttcaa cggccgcgcg gcctatctcg cggcgccacg
      481 tgtgcgaacg cgtgttggag tggagctgga tgttgaaacg cccgcgccgc gcacgccgct
      541 ccgtcgcaac ggccaacttg tgggcgaaac gatgagttcg ctttattcgc ccgcgcttca
      601 gcgcgcaatc gcgcttgccg tcgtcaaggt ctcggatagc gaacagggca ctgtgcttgt
      661 cctgccggat ggccggtcgg cgcgcgtttg cgctcttcca tttctgccag tgccatccga
      721 cggaaacggc actgcgccgc gttaaatccc ggaaagccct tttgcgggcg gattgcgcgc
      781 gcacccacgg catggttgga agccggttga gatgatcgat ccggataccg acctcgttgc
      841 ccgggctggg cagggcgacc gtgccgcggc gcaagccttg atggcgcggc atctgcccaa
      901 aatgctcacg gtcgcgcgcc ggatgctttc cagccaggcc gaagccgaag acgccgtgca
      961 ggacgcgttc ctgaaagtct ggacccacgc gtcgcgctgg cagcccggca aggcgctgtt
     1021 cgagacctgg ctgtaccggg tcgtgatgaa caagtgctac gaccgcctgc gcaaacggcc
     1081 caacgccagc ctggatgagg cggtcgaggt tccggacgtc gcgccgcagg ccgacgcagt
     1141 catggaaaat acggcacttg gcaaggaaat cgaggtggct ctggccgcgt tgcccgaacg
     1201 ccagcgggcc gccatcgtgc tgtgccacca tcaggaatgc ggcaatatcg aagcggcgga
     1261 gattctgggg atcagcgttg aagcattgga gtccctgttg tcgcggggcc gcaggacact
     1321 cagagctcgg ttggctcatc tgaaggacgg ttaggagaac ggggacatgg aaaatttacc
     1381 aaacgaagac gccatctggg tcaccgacgc gctgaaggct ttgccgacgg ttccggtttc
     1441 cgcggcgctt cagcaaaata tcctcgcctc tttcgaggcc gtgacggcgc gccgcaatgc
     1501 gggtttcgct ggcgcggtgc gcaggcttgc ggcgaccgta tggccgggcg tgcctgcctg
     1561 gcgtccggcg accgcgctcg cggtatcgct tgtgatcggc cttgccgccg gaacgctcgt
     1621 tccgctggaa ggcgcgatgg ccgacaacaa cgaacagacc gccagcgttg tgctcgatgc
     1681 gccaccctct ttcgatctcg gtgagaattc gtgaaatgag cgatcctgtt gtcacgccga
     1741 aacgccagcc gagcgtgctg ctgattga
//
LOCUS       DASZMC010000002         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3298373,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000002 DASZMC010000000
VERSION     DASZMC010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298373"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VG274_00020"
     CDS             <1..395
                     /locus_tag="VG274_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWE05060.1"
                     /translation="GATGVLFAKGSASPFHPRTLRASAGSLFRVPFVAGVDAALARAT
                     LQQRRVKIFAAMPFGESRQIAGDADFERKCALVIGSEGRGISNELYGIAEDVAIPTTG
                     VESLNAAVAASVLLYEARRQRQREAVRA"
     gene            392..1699
                     /locus_tag="VG274_00025"
     CDS             392..1699
                     /locus_tag="VG274_00025"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWE05061.1"
                     /translation="MSLFDASPLASFEPKDDPKRPLADRMRPETLDDYAGQEHILGPG
                     KPLRSQIERDQLTSIILWGPPGVGKTTLARIIARMTRSEFLPFSAVLSGIKEIKTVMA
                     EAERLRRMGKRTILFIDEIHRFNKAQQDAFLPYVEHGDIVLIGATTENPSFEVNAALL
                     SRSKVYMLRALTEEEIAELLQRALGLLRVEAGEETIAKIAAGANGDARNAYNTLEIAA
                     AASADGLVTEQTVEDALGRKVLLYDKSGEEHYNLISALHKSVRSSDPDAALYWLARML
                     EAGEDRMYIARRVVRMSIEDIGLADPRALEQCMAMQQAVHFLGIPEGDLALAQAVIYL
                     SITPKSDAAYRALGDVIGEVRSGRAEPVPMQLRNAPTGAMKAWGYGAGYQHAHKFEDA
                     MNEMECLPESLAGKRWYFPAERGLEKRIKERLEEIRAARGKSD"
     gene            1778..2398
                     /locus_tag="VG274_00030"
     CDS             1778..2398
                     /locus_tag="VG274_00030"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWE05062.1"
                     /translation="MPSYSAALRPRRAPRQERGERRVASLLDAAEFVIAEHGYEAATM
                     SEIADKAGASIGSLYQFFPSKQAIAQALRTRYKEAYDRRWLPIERDFVRLDLSEFASR
                     LMDMAIVFMNNHPASAALLDAPRSTRIQGSMVERVANFLLVKKPRMPAKSAARIASVT
                     VHMVRAFNQAWVEAAAQARRSLKREFHMALVCYLTSRLKQERGNGR"
     gene            2395..>3622
                     /locus_tag="VG274_00035"
     CDS             2395..>3622
                     /locus_tag="VG274_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWE05063.1"
                     /translation="MTVRPRAATALVFLGVLASGVLALLLAGCSGKSETTAQRQPPPP
                     TVGVVTVQQRDVPTYGDWVASLDGFVNANIQPQVSGYLLRQIYREGSFVRKDQVLFEI
                     DPRPFQAALDQARGQLAQARGQLAQARGQLAQSQAQLQLTEINVNRDQPLVQARAVPK
                     SQLDTDVQAREQNKALVQTAQASIQTAQAAIQAAEAAVETASLNLGFTKVRSLIDGIA
                     GIASTQIGNLVGQTTVLTTVSQVNPIKVYFPISEQEYLRLSGRIHGNAPDLLRATSRV
                     PLEMTLANGDKYARQGRIIFADRQVDTQTGTIRMVGQFSNPGNILRPGQFARIRAMTG
                     YNRGALLVPQRAVTEVQGRYQVAVVGPDNKVAIHNVQAGERVDDMWIISSGLAANDRV
                     VSEGTSKVRDGVVVNPQ"
BASE COUNT          683 a         1103 c         1211 g          625 t
ORIGIN      
        1 tcggcgcgac gggcgtgctt tttgcgaagg gcagcgccag cccgtttcat ccgcgaaccc
       61 tgcgggcttc ggcggggtcg ctgtttcgcg tgccgtttgt ggcaggcgtg gacgcggcgc
      121 tggcccgagc caccctgcaa cagcggcgcg tgaagatttt cgccgccatg ccgtttggag
      181 aatcgcgcca gatcgccggc gatgcggatt tcgagcggaa atgcgccctc gtgatcggca
      241 gtgaagggcg gggaatcagc aacgagcttt atggcatcgc ggaagacgtg gcgattccca
      301 cgacgggcgt cgagtcgctg aatgccgcgg tggcggcttc cgtgctgctc tatgaagcgc
      361 ggcggcagcg gcagcgcgag gccgtgcgcg cgtgagcctt ttcgatgcga gcccgcttgc
      421 gagctttgag ccgaaggacg acccgaaacg tccgctggcg gaccggatgc gtccggaaac
      481 gctcgacgac tacgccgggc aggaacatat tctgggcccg ggaaagccgc tgcgctcgca
      541 gatcgagcgg gatcagctca cctccatcat tctttgggga ccgccgggcg tgggcaagac
      601 gacattggcg cgcatcatcg cgcgcatgac gcgcagcgag tttctgccct tcagcgccgt
      661 gctcagcggc atcaaggaaa tcaaaaccgt gatggcggag gccgagcggc tgcgccgcat
      721 ggggaagcgg acgatcctgt tcatcgacga gatccaccgc ttcaacaaag cgcagcagga
      781 tgcgttcctg ccctatgtgg agcacggcga tattgttctg atcggcgcca cgacggagaa
      841 tccatcgttc gaagtgaacg cggcgctgct ttcgcgctcg aaggtgtaca tgctgcgggc
      901 gctcaccgaa gaggagatcg cggagctgct gcaacgcgcc ctcgggctgc tgcgggtgga
      961 agcgggcgag gagactattg cgaagatcgc cgccggggcc aatggcgatg ctcgaaacgc
     1021 ctacaacacg ctcgaaatcg ccgccgccgc gagcgcggac gggctggtga cggaacagac
     1081 cgtggaggac gcgctgggcc gcaaggtgct gctctacgac aaatccggcg aagaacatta
     1141 caatctgatc tccgcgcttc acaaatcggt gcggtcgagc gatcccgacg ccgcgctcta
     1201 ctggctggcg cgcatgctgg aagcgggcga ggaccggatg tatatcgcgc ggcgcgtggt
     1261 gcgcatgtcg attgaggata tcggccttgc cgatccgcgc gcgctcgagc agtgcatggc
     1321 gatgcagcag gccgtgcact tcctcggcat acccgagggg gatctggcgc tggcgcaggc
     1381 cgtgatctat ctttcgataa cgccgaaatc ggacgcggcg tatcgcgcgc tcggcgacgt
     1441 gatcggcgaa gtgcggtccg gccgcgcgga gccggtgccc atgcaattgc gcaatgcccc
     1501 caccggcgcg atgaaggcgt ggggctatgg ggcgggctat cagcacgcgc acaaattcga
     1561 agacgccatg aatgaaatgg aatgcctgcc cgaatccctc gcgggaaagc gctggtactt
     1621 cccggcggag cgcggcctcg agaagcgcat caaagagcgg ctggaagaga ttcgggcggc
     1681 ccgcggcaag tccgattgag attcagccgc tcgccgtatc atcccacact tgcgccccgg
     1741 aatatgagtg agtgctcata ttagactaaa cacgagcatg ccctcatatt cggcggcgct
     1801 tcgtccccgc agagcccctc ggcaggaacg gggagagcgg cgggtcgcgt cgcttctcga
     1861 cgccgccgag ttcgtcatcg cggaacacgg ctatgaagcc gccaccatga gcgagatcgc
     1921 ggataaggcc ggggcgtcga tcggctccct gtatcagttt tttccatcga aacaggcgat
     1981 cgcccaggca ttgcgcaccc gttacaaaga agcgtacgac cggcgctggc tgcccatcga
     2041 gcgggatttc gtccggctgg atctttccga gttcgcttcg cgtctgatgg acatggcgat
     2101 cgtcttcatg aacaatcacc ctgcttccgc cgccttgctg gatgcgccgc gttccacgcg
     2161 aattcagggc tcgatggtgg agcgggtggc gaattttctg ctggtaaaga agccccggat
     2221 gccggcgaaa tccgcggccc ggatcgccag cgtgacggtt cacatggtga gggctttcaa
     2281 tcaggcttgg gtggaagcgg cggcgcaggc gcggcgctcg ctcaagcgcg agtttcacat
     2341 ggctctggtt tgctatctca caagccggct caaacaggaa aggggaaacg gccgatgacg
     2401 gtgcgtccac gcgcggcgac cgcgctggtt tttttggggg tcctcgcgtc aggagttctg
     2461 gcgctgctgc tcgcgggatg ttcgggcaag tcggaaacca ccgcgcagag acagcctcca
     2521 ccgccgacgg tcggcgtcgt cacggtgcag cagcgcgatg tgccaactta cggagactgg
     2581 gtggcgagcc ttgacggctt cgtcaacgcc aacattcagc cgcaggtttc cggatatctt
     2641 ttgcggcaga tctatcgcga agggtcgttc gtccgcaagg atcaggttct tttcgaaatc
     2701 gacccccgtc cgtttcaggc ggctctcgat caggcgcgcg gacaactggc gcaggcgcgc
     2761 ggacaactgg cgcaggctcg cggacaactg gcgcaatcgc aggcgcagtt gcagctcacg
     2821 gaaatcaacg tgaaccgcga tcagccgctc gttcaggcgc gcgccgttcc gaagagccag
     2881 ctcgacaccg acgtgcaggc gcgtgagcag aataaggcgc ttgtccagac cgcgcaggca
     2941 tcgatccaga ccgcgcaggc ggcgatccag gcggcggagg cggcggtcga gacggcttcg
     3001 ctgaatctcg ggttcacgaa agtccgatcg ctcatcgatg gcatcgccgg catcgccagc
     3061 acgcagatcg gcaacctggt tggccagacg acggtactga ccacggtctc ccaggtgaac
     3121 ccgatcaagg tgtatttccc catcagcgaa caggagtatc tgaggctttc cggacgcatc
     3181 catggaaacg ctccggacct gcttcgcgcc accagccgcg ttccactgga aatgacactg
     3241 gccaacggcg acaagtacgc gcgccagggc agaatcatct tcgccgatcg ccaggtggat
     3301 acgcagaccg gcaccatccg catggtgggc cagttctcca atcccggcaa catcctgcgg
     3361 cctggccagt ttgcgcgcat tcgcgccatg accggctata accggggcgc gctgctggtg
     3421 ccgcagcggg ccgtcaccga ggtacagggg agatatcagg tggccgttgt gggtccggat
     3481 aacaaggtcg ccatccacaa cgttcaggca ggcgagcgcg tggacgatat gtggatcatc
     3541 tcgagcggcc tcgccgccaa cgatcgcgtg gtttccgaag gcacttccaa agtgagagac
     3601 ggagttgtgg tgaacccgca gc
//
LOCUS       DASZMC010000003         5241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2474296,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000003 DASZMC010000000
VERSION     DASZMC010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474296"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VG274_00040"
     CDS             <1..260
                     /locus_tag="VG274_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05064.1"
                     /translation="DLADKFHMTLTGMKKHVGVLEQAGLVTTKKVGRVRTCKLGLRRL
                     DEEAAWIERYRQLWAARFDALDEIVEELKRKEKADGRKKRK"
     gene            270..746
                     /locus_tag="VG274_00045"
     CDS             270..746
                     /locus_tag="VG274_00045"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWE05065.1"
                     /translation="MKNSTTTERTSGREVVTTRSFNGPARIVFEAWTRPELLMRWWAP
                     KSLGISFISCEIDARTGGSYRFVFGHSASEQPMAFFGRYIEVTPPSRLVWTNEEGGEG
                     GAITTVTFEERDGETLVVMHDLYPSKEALDEAIASESMGDYSETFTQLDELLSARA"
     gene            complement(757..1698)
                     /locus_tag="VG274_00050"
     CDS             complement(757..1698)
                     /locus_tag="VG274_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HWE05066.1"
                     /translation="MSTRPNLLMVVPLTWAGLWLPFLSEANLNVFVHGRDDYKQDDID
                     YVLSFRPPPGFLKTLPKLKTVYSLGAGVDGFLADPDFPKQIPLVRFVDPQLSTEMAQY
                     VVLHVLLQHRHAKAFEETQKQSKWAQMMLPRKTADTRIGVLGLGEIGTMAGERLRDLG
                     FPVSGWSRSRKQVGGIKSYAGEDEFTAFMGASDFLICLLPLTDDTRGILNAKTFAMLP
                     KDSYVINVARGGHLIEGHLIAALDSGHLAGATLDVFQTEPLPETSPLWKHPKIVVTPH
                     VAAISDPRVAANFVIDGIAHAERGEKHPNTVDMSRGY"
     gene            complement(1741..2439)
                     /locus_tag="VG274_00055"
     CDS             complement(1741..2439)
                     /locus_tag="VG274_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HWE05067.1"
                     /translation="MDSFEWNKIAGAVLGTLIFVLVIKFGTEAIFEVPAPAKPGYVVE
                     GVTEEASTASAAPVEEAIPDWGTVLPKADAAAGQKISARCEQCHDLSKGGPNKIGPNL
                     WGVLGRPRGTHPGFSYSGAMTSSHDVWSLDNFFKFIKSPAAYVPGTKMSFAGLRSADD
                     RINLIAWLRTQADNPLPIPAPKPAAAAPAAPADGKAPTTTAPTGTSTTAAPNSPATPA
                     KATTPPAPAAPKKN"
     gene            2575..3309
                     /locus_tag="VG274_00060"
     CDS             2575..3309
                     /locus_tag="VG274_00060"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HWE05068.1"
                     /translation="MKPIVLIPARMASTRLPGKPLADIAGIPMIVRVWRQAVVADLGP
                     VVVAAAEYAIVAAIEGAGGRAILTAPDLPSGSDRIYAALEEIDPRGAHDVVVNLQGDL
                     PALDPAYVRTVADTLIEAGTDIATLAAEIDDDADRDNPNVVKPVVAWEGERGRALYFT
                     RARAPHGDGALFHHVGIYAYTRDALTRFVKLAPSPLERREKLEQLRALEAGMTIAVAR
                     VGAVPLSVDTPEDLQKARDLISREGN"
     gene            3311..4210
                     /locus_tag="VG274_00065"
     CDS             3311..4210
                     /locus_tag="VG274_00065"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWE05069.1"
                     /translation="MTSLTARAKTIAFQGEPGAYANLSAREAVPHATAIPCPTFDAAL
                     EAVREGKADLAIVPVENSVYGRIADTHLLVRHFGRPVKGHASGEKNDLFIVGEHFHRV
                     RHQLLGLKGAALAGVKEVYSQRPALGQCQKIIKELKLVEREWYDTAGSAKHIAGLGDP
                     KCAAIASSLAGELYGLDILRADIEDEAHNMTRFLIVGGEDNDAPNDGHAVITTLLFRV
                     KNIPAALYKALGGFATNGVNMTKLESYQLGGSFNATEFYADIEGHPDQPHVRRALEEL
                     QFFTSELTTLGVYPAHLFRAEMP"
     gene            4431..4826
                     /locus_tag="VG274_00070"
     CDS             4431..4826
                     /locus_tag="VG274_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05070.1"
                     /translation="MPKRFTVFAVAMLLSSPALAADLRGEIMNAAEHAEYSGEATTIG
                     DAHMHLHHSLNCLVGPNGEGFDPHSLNPCVHSGNGVIPDTTDAKLIAVYRSAAAKCRE
                     GLATNDIAVEQSDAIAAAKLIVATLKSVK"
     gene            complement(4827..>5241)
                     /locus_tag="VG274_00075"
     CDS             complement(4827..>5241)
                     /locus_tag="VG274_00075"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HWE05071.1"
                     /translation="TDPVVIMLGIHGDACLVGRNKRFPGNFYSALAGFMEPGETIEEA
                     VRRELMEEVNLKVGAVRYVATQPWPFPSSLMIGCLAEAESRDFKPDGEEISDARWLSR
                     DDAKRLIGGESLPDIAVPPSIAIAHHLIKAWVAGA"
BASE COUNT         1003 a         1625 c         1649 g          964 t
ORIGIN      
        1 cggaccttgc cgacaagttc cacatgaccc tcacgggcat gaagaagcat gtcggcgtcc
       61 tggagcaggc ggggctcgtc accacgaaaa aggtcgggcg cgtgcgcacc tgcaagctcg
      121 gcctgcgccg gctggacgaa gaggcggcgt ggatcgagcg gtaccgccag ctctgggccg
      181 cgcgcttcga cgcgctggat gaaatcgtcg aagaactgaa acgtaaagag aaggccgatg
      241 gccgcaagaa gagaaagtga gcgcacccca tgaaaaacag cacgacaacg gaacggacgt
      301 ccgggcgcga ggtggtcacg acgcgaagct tcaatggccc ggcgcgcatc gtgttcgagg
      361 cgtggaccag gcccgagctg ctcatgcgat ggtgggcccc gaagtcactt gggatatcct
      421 tcatttcctg cgagatcgac gcgcgtacgg gcggcagcta ccggttcgtg ttcggccact
      481 cggcctcaga gcagcccatg gcgttcttcg gcagatacat cgaagtgacg ccgccctccc
      541 gcctcgtctg gacaaatgaa gaaggtggcg aaggtggcgc catcacaacg gtgacgtttg
      601 aagaaagaga tggcgagacg ctggtcgtca tgcacgacct ctatccctcg aaggaagcgc
      661 tcgacgaggc catcgcctcc gagagtatgg gtgactacag cgagacattc acgcaactgg
      721 acgaacttct ttccgcaaga gcctgaccga acgcggctag tatcctctgc tcatatcgac
      781 agtatttgga tgtttctcgc cgcgttcggc gtgggcgatg ccgtcgatga cgaagttcgc
      841 cgcgacgcgg gggtcggaga tcgcggcgac gtgcggtgtg acgacgatct tcggatgttt
      901 ccagagcggg cttgtttccg gcaacggctc cgtttggaag acgtcgagcg tcgcgccggc
      961 gagatggcca ctgtcgagcg ctgcgatcag gtggccttcg atcagatgtc cgccgcgcgc
     1021 gacattgatc acataggaat ctttcggcag catcgcgaac gtcttcgcgt tcaggattcc
     1081 acgcgtgtcg tcggtcagtg gcagcaggca gatcaggaaa tcgctcgcgc ccatgaaggc
     1141 cgtgaattcg tcctcaccgg cataactctt gatgccaccg acctgtttgc gcgaacggct
     1201 ccagcccgag accggaaagc ccagatcgcg cagacgttcg cctgccatcg tgccgatctc
     1261 gccgagaccc agcacaccga tgcgcgtgtc cgcggtcttt cgcggcagca tcatctgcgc
     1321 ccatttgctc tgcttctgcg tctcctcgaa cgccttggcg tgacggtgct gcagcagcac
     1381 gtgcagcacg acgtactgcg ccatctccgt cgacaattgc ggatcgacaa aacgcaccaa
     1441 cggaatctgt ttcggaaaat cgggatcagc gagaaagccg tccacaccgg cgccaagcga
     1501 atacaccgtc ttgagtttcg gcagcgtttt cagaaagccc ggcggcggcc gaaagctcag
     1561 cacataatcg atgtcgtctt gtttgtagtc gtcgcggccg tgcacgaaga cattgagatt
     1621 cgcttcgctc aaaaacggca gccacagacc cgcccatgtc agcggaacga ccatcaaaag
     1681 attggggcgt gtgctcatgc gcacacgctc cgtgcttttg ccgtttagat caagcccgcg
     1741 tcaattcttc ttcggtgcgg ctggcgcagg cggcgttgtt gccttggccg gtgtcgccgg
     1801 gctgttcggc gcggcggtgg tactggtgcc agtgggagcc gtcgtcgtgg gtgctttgcc
     1861 atcagccggt gccgcaggtg ctgcggccgc cggcttcggc gccgggatcg gcagggggtt
     1921 gtccgcctgt gtgcgcaacc acgcgatcag gttgatgcgg tcgtctgcgc tgcgaagacc
     1981 ggcgaagctc atcttcgtgc ccggcacata agccgccggc gatttgatga acttgaagaa
     2041 attgtccagc gaccagacat cgtggctcga cgtcatcgcg ccggaatagg agaagcccgg
     2101 atgcgtgcca cgcggacggc ccagaacgcc ccacaggttc ggaccgatct tgttcggtcc
     2161 acccttcgac agatcgtggc actgctcgca acgcgcggag atcttctgac ccgccgctgc
     2221 gtcagctttc ggcagcacgg tgccccaatc cggaatcgct tcttcaacgg gcgccgcact
     2281 cgctgtagac gcttcttccg tcacaccttc gacgacatag ccgggcttgg ccggggccgg
     2341 cacttcgaag attgcttcgg tgccgaattt gatgacgaga acaaagatca atgtgcccag
     2401 aaccgcgccg gcgatcttgt tccactcaaa ggaatccata aggtctgctc cgctcgtcgc
     2461 gcgtgaaagg gcgccgacgc gcccgtatga ggatcgcggc gacattacaa gtccgcccaa
     2521 ttgcgtcatt gcggcgaagc gtcgcataaa tcaaggaaat cccggctttt ccccatgaaa
     2581 cccatcgttc tgatccccgc ccgcatggcc tccacgcgcc tgccgggtaa accgctggcc
     2641 gatatcgcgg gcattcccat gatcgtgcgg gtctggcgtc aggccgttgt ggcggatttg
     2701 gggcctgtgg tggtggccgc cgcggagtac gcgatcgtgg ctgcgatcga aggcgcgggc
     2761 gggcgcgcga tcctgaccgc gcccgatctg ccgtctggct cggaccggat ttatgccgcg
     2821 ctggaggaga tcgatcccag gggcgcacac gatgtcgtcg tcaatctgca gggcgatctg
     2881 ccggcgctcg atccggctta tgtgcgcaca gttgccgaca cgttgatcga ggcgggcacg
     2941 gatatcgcga cgctcgccgc cgagatcgac gacgacgccg accgcgacaa ccccaatgtc
     3001 gtgaagccgg tcgtggcgtg ggaaggcgag cgcggccgcg cactctattt cacgcgcgcg
     3061 cgggcgccgc atggtgatgg ggcgctgttt catcatgtcg gcatctatgc ctatacgcgc
     3121 gatgcgctga cgcgcttcgt gaagcttgcg ccgtcgccgc tggagcggcg cgaaaagctg
     3181 gaacagctgc gggcactgga agccggcatg accatcgcgg tcgcccgcgt cggcgccgtg
     3241 ccgctcagcg tcgacacgcc ggaagattta cagaaagcgc gcgatctgat ttcgcgcgaa
     3301 ggcaattgag atgacatcgc tgaccgcgcg cgcgaagacc atcgcctttc agggcgagcc
     3361 cggcgcctat gccaatctct ccgcgcgcga ggccgtgccg catgcgacgg cgatcccgtg
     3421 tccaaccttc gatgccgcgc tcgaagcagt gcgcgaaggc aaggccgatc tcgccatcgt
     3481 gccggtggag aattccgtct atggccgcat cgccgacacg catctgctcg tgcgccattt
     3541 cgggcggccg gtgaaaggtc acgcttcagg agagaaaaac gatctcttca tcgtgggtga
     3601 gcatttccac cgcgtgcgcc atcaattgct cgggctgaag ggtgcagcgc tcgccggtgt
     3661 gaaagaggtc tacagccagc gtcccgcgct cggccaatgc cagaagatca tcaaggaact
     3721 gaagctggtc gagcgcgaat ggtacgacac ggcgggcagc gcgaaacata tcgccggact
     3781 gggcgatccg aaatgcgccg cgattgcatc gtcacttgcg ggcgaactct acgggctcga
     3841 catcctgcgc gccgatatcg aggacgaggc gcacaacatg acgcgcttcc tgatcgtcgg
     3901 cggcgaggac aacgacgcgc ccaacgacgg ccatgcggtg atcacgacgc tgctgtttcg
     3961 cgtaaagaac atccccgccg cgctctacaa agcgctgggc ggcttcgcga ccaatggcgt
     4021 gaacatgacg aagctcgaat cctatcagct cggcggctcc ttcaacgcga cggaattcta
     4081 tgccgacatc gaaggccacc ccgaccagcc gcacgtgcgc cgcgcgctgg aggagcttca
     4141 gtttttcacc tcggaactca cgaccctggg agtctatccc gcccatctgt tccgcgcgga
     4201 aatgccatag tcctttccaa aacgttgcgt cccggatagg tccgcagaac cgcggcggtc
     4261 tgtatcaatg acggcaatgt cgtgatcggc acttattatc aggtcacggg accgcatacc
     4321 ggaacgcaga tgggattcat ccgctcccaa taaggcaggc ttggcgctgt tcaacaccgt
     4381 gccgtggcaa acgcctgtgc tatgaatacg agaatcttgg ggttcgggtc atgccgaaac
     4441 gtttcacggt gttcgcagtg gcgatgcttt tgtcgtcgcc cgcgctggcc gccgatctgc
     4501 gcggtgagat catgaatgcg gcggagcatg ccgagtattc cggcgaggcg acgaccatcg
     4561 gcgacgcaca catgcatctg catcattcgc tgaactgtct cgtcggcccg aacggcgagg
     4621 gcttcgatcc gcattcgctg aatccctgcg tgcattccgg caacggcgtc attcccgaca
     4681 cgaccgatgc gaaattgatc gccgtgtacc ggagcgctgc cgcgaagtgc cgcgaaggtc
     4741 tcgccaccaa cgatatcgcc gttgagcaaa gcgacgccat cgccgccgcc aaactcatcg
     4801 tcgcgacgct gaagagcgtg aaatagctac gcgcccgcga cccacgcctt gatgaggtga
     4861 tgcgctatgg cgatggaggg gggcacggcg atgtcaggca atgactcgcc gccgatcagg
     4921 cgtttcgcat cgtcgcggct tagccagcgg gcgtcgctga tctcttcgcc gtcgggtttg
     4981 aagtcgcggc tttcggcttc ggcgagacag ccgatcatta gcgaggaagg aaacggccac
     5041 ggctgcgtcg cgacgtagcg cacggcgccg actttcagat tcacttcctc catcaattcg
     5101 cggcgcacgg cttcctcgat ggtctcgccg ggctccatga agcccgcgag cgcggaatag
     5161 aaattccccg ggaagcgctt gttgcgtccg accaggcacg catcgccatg gatgccgagc
     5221 atgatgacga caggatcggt g
//
LOCUS       DASZMC010000004         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_550504,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000004 DASZMC010000000
VERSION     DASZMC010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550504"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2564
                     /locus_tag="VG274_00080"
     CDS             <1..>2564
                     /locus_tag="VG274_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05072.1"
                     /translation="SFGLYVGKTPERSADVPGVRLQQGASPADHEAELRRLDQAREVR
                     AVHPYEERPSREELRGDPPRILLTNVKQLELLLTRQKDIEMFRGSRLDYLVFDETHTF
                     RGANGAETACLVRRLRSFCGRTAAETVCIGASATIAGSDGGNDEGIRFATRFFGVPAD
                     RVEIIREEFEPDSWSDPRQISEPFPGDPNVRFDNVLHALRRLEEEPTSVDAKERFREV
                     FEILSGRRVDIRRLEESLYEHLSSNELLFQASDLLQTAQSLGEFCESLGARIGRSVPV
                     EEALTWMALGAFARSNGRTLVRPVIHGFVRGVGGAVVTFPGRGSGLGDAEPRLWLSAE
                     DHANSDQADFYRLPVTTCTTCGQQYFVHSLRDFEFLDRQPMGGEAVETQYMWRPQSEA
                     NGGRRIVLLDRLTIREGEDDDDGTAGPPRNTVAVYLCRRCGTVHPQRLDRCDGCGRAA
                     ASNGGDASPIMVELFVVQQRRETIGRLQSCVACRATGHWAASGHREPARPVRAVNVSD
                     VFVLAQSMIEHADHRRLLVFADNRQEAAFQAGWMQDHARRYRLRSLMYSRIRQADASN
                     GQAGVSVGDLTAWLDQELARNDDESRSLAPEVWRVARQEAAGNRHAQERFCFLRIQVL
                     RELTMGHNQRIGLEPWGRMVVEYIGLNPDLPFFTQWAARLGSTPLELREGVAALLDVT
                     RRARVLLDREGRIFSRYWRADEREVSYGYLPFSEQPPIGLKLQLGGQDNPGFVKQWLS
                     QRGQTAARHAARRWGVPTDQVATFFEELWDLLANQLHILAPANLTGSSNRALPGSVGA
                     MQIDADRVLMVPHQGLWRCDVCRRRHVHATPNRACMVNRCAGTLVFEREEEDNYDL"
BASE COUNT          486 a          736 c          854 g          488 t
ORIGIN      
        1 tcgttcggat tgtatgtggg caaaacaccg gaacggtccg ccgacgttcc gggcgtccgt
       61 ctgcaacagg gagcttcgcc ggccgaccac gaagccgaat tgcgcaggct cgatcaggcg
      121 cgagaggtcc gagccgttca cccgtatgaa gagcggccct cgcgtgaaga gctgcggggc
      181 gatccgccgc gcatcctgct gacaaacgtc aagcagctcg aactgctgct gacgcggcag
      241 aaagatatag agatgttccg gggtagccgc ctggattatc tggtgtttga cgagacgcat
      301 acgttccgag gagcaaacgg ggcggaaacc gcgtgcctcg tgcgcaggct gcggtcgttc
      361 tgcgggcgca cagccgccga gacagtctgc atcggggcct cggcaacgat cgcgggcagt
      421 gacggcggga acgacgaagg aattcgtttt gccacaagat tcttcggcgt acccgcggat
      481 cgcgtggaga ttatacggga agagttcgag cccgattcct ggtcggaccc gcgccagata
      541 agcgagccat ttcccggtga cccgaatgtt cgcttcgata acgtcctcca cgcccttcgg
      601 cggcttgaag aggaaccgac ttcggttgac gcgaaggagc gtttccgtga ggtgttcgag
      661 attctttcgg gccggcgtgt tgatatccgg cgtctggagg aaagtctcta cgagcacctt
      721 tcatcgaatg agcttttgtt ccaggcaagc gatctactgc agacggcgca gtcgcttggc
      781 gaattctgcg aaagcctggg cgctcgaatc ggccgcagcg ttccggtgga agaagccctg
      841 acctggatgg cgctgggcgc gttcgcacgg agcaacggaa ggacactagt gcgtccggta
      901 atccacgggt ttgttcgcgg ggtcggcgga gcggtggtta cctttcctgg ccgtggctcc
      961 ggtttgggag atgccgaacc gcgactttgg ttaagcgccg aggaccacgc caactcggac
     1021 caggccgact tctaccgctt gccggtgacg acatgcacaa cctgcggcca gcagtatttt
     1081 gtccactcgc tccgggattt cgagttcctg gaccggcaac caatgggcgg cgaggcagtc
     1141 gagacccagt acatgtggcg tccgcaatca gaggcaaatg ggggccggcg catagtgttg
     1201 ctggaccgac tcacgattcg cgagggcgag gacgatgacg acggtacggc gggacctccg
     1261 agaaacacag tggcggtcta tctctgccgt cggtgtggaa cggtgcatcc ccagcgactc
     1321 gatcggtgcg acggttgcgg gcgggcggcg gcttcgaacg gtggcgatgc tagtccgatc
     1381 atggtcgagc tgttcgtcgt ccagcagagg cgggaaacga tcgggcggct tcagtcctgc
     1441 gtcgcctgcc gggccaccgg ccattgggcc gcaagcgggc accgggagcc tgcgcgaccc
     1501 gttcgagcgg tgaatgtctc cgacgtgttc gtgctcgcgc aaagcatgat cgaacatgcc
     1561 gaccatcgca ggctacttgt cttcgccgac aaccgccagg aagccgcgtt ccaggcaggg
     1621 tggatgcagg accacgcgcg ccgctatcgg ctgcggtcgc tcatgtattc acgaatccgg
     1681 caggccgacg catcaaacgg acaagctggt gtatcggtag gcgacctgac ggcatggctc
     1741 gatcaggaac tggctcgcaa tgatgacgag tcgcgctccc ttgctccgga agtctggcgg
     1801 gtcgctcggc aggaagctgc gggaaaccgg cacgctcagg agcgattctg cttcctccgg
     1861 attcaggtgc ttcgcgagct cacgatggga cacaatcagc ggattggtct cgaaccgtgg
     1921 ggccgcatgg tcgttgagta cattggcctc aatccggacc ttccgttttt cacgcaatgg
     1981 gccgcgcggc ttggatcgac acctttagaa ttgcgcgagg gtgttgcggc cttgctcgat
     2041 gtcactcggc gcgcgcgggt cctgctcgat cgtgaaggac gtatattcag ccggtactgg
     2101 cgggcagacg aaagagaggt ttcttatgga tacctaccgt tttcggaaca accgccgatc
     2161 gggctgaagc ttcaactcgg gggacaggac aatccgggct tcgtgaagca gtggttaagt
     2221 caacgcggac agacggcagc tcgccatgcc gcgaggagat ggggcgtccc gaccgatcag
     2281 gtcgcaactt ttttcgaaga attgtgggac ctgctggcga atcagctgca catcctcgcc
     2341 ccggcgaacc tcactggttc cagcaatcgg gcgctgcccg gatcagtagg cgctatgcag
     2401 atcgatgccg accgagtgct gatggtgccg catcagggtt tatggcgttg cgacgtctgt
     2461 cggcgccgcc atgtccacgc gacgcccaac agggcttgca tggtgaaccg ttgcgcggga
     2521 acgctggtct tcgagcgcga agaagaagac aactatgacc tgca
//
LOCUS       DASZMC010000005         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2475819,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000005 DASZMC010000000
VERSION     DASZMC010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2475819"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VG274_00085"
     CDS             complement(<1..105)
                     /locus_tag="VG274_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWE05073.1"
                     /translation="MQRRGYIAVEGPIGIGKTSLARLLAHELGARVIFE"
     gene            complement(199..1143)
                     /gene="ispH"
                     /locus_tag="VG274_00090"
     CDS             complement(199..1143)
                     /gene="ispH"
                     /locus_tag="VG274_00090"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017387043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWE05074.1"
                     /translation="MLPSETSIQKIILAEPRGFCAGVDRAVEAVRGALREYGRPLYVR
                     HQIVHNRFVLEALEQEGAIFVENLDDVPEAQRVIFSAHGVSPSEWEHARDRGLRVIDA
                     TCPLVTKVHLEVERYAAEDRTVILIGHAGHEEVKGTLGVAPGRILLVGTVEEAEQIEV
                     PDPQRIAAVTQTTLSVDDTRDIMEMLKRRFPALATPKTDDICYATQNRQNAVKELVGA
                     TDAILVVGSKQSSNANRMVEVARMRGARAFLVDSLADVQPEMLSGVRSLGLTASASSP
                     EWLVEEIIGAFSSRGAAVELMKLKEERIHFPLPKPAEP"
     gene            complement(1250..>1700)
                     /locus_tag="VG274_00095"
     CDS             complement(1250..>1700)
                     /locus_tag="VG274_00095"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWE05075.1"
                     /translation="AVVELAVIRHPGASAIVALDAEGTIAMLTQYRHAIGGWLREIPA
                     GCRNGSETALECARRELREEAGLAAARWDHLGSLVTIPSFCDERIELFLARELSPANG
                     ELDYDEVIRVERVKLEDALAMVRRGEIIDAKTIAALHHAAVFAGSDC"
BASE COUNT          301 a          593 c          492 g          314 t
ORIGIN      
        1 ttcaaaaatg acgcgggcgc ccagttcgtg cgccagcagc cgcgccaggc tggtcttgcc
       61 gatgccgatc ggtccttcga ccgctatata cccccgccgc tgcatcgagc cgcctccatc
      121 aactccctcc cccgaaggcg aacggaccac ttgtcgccag ccgcagcaat tggcggccgt
      181 ccgcacatgc gcgaaatctt acggttcggc cggtttaggc agcggaaaat gaatacgctc
      241 ctccttcagc ttcatcagtt cgactgccgc gccgcggctg gagaacgcgc cgattatctc
      301 ctcgaccagc cattccggcg acgaggcgct cgccgtcagt cccagcgagc gcaccccgga
      361 gagcatctcg ggctggacat cggcgagcga atcgaccagg aacgcgcgcg caccccgcat
      421 ccgcgcgacc tcgaccatcc ggttggcgtt ggagctttgc ttcgagccga ccaccaaaat
      481 cgcgtcggtc gccccgacca attccttgac cgcgttctgg cggttctgcg tcgcgtaaca
      541 gatatcgtcg gtcttgggcg tcgccagcgc cggaaaccgc cgcttgagca tctccatgat
      601 gtcgcgggta tcatccacgc ttagcgtcgt ctgggtgacg gcggcgatcc gctgagggtc
      661 gggaacctcg atctgttcgg cctcctcgac cgtgccgacg agcaggattc tgccgggtgc
      721 gacaccgagc gtacccttga cctcctcatg gcccgcatgg ccgatcagga tgacggtacg
      781 atcctcggcg gcgtagcgtt cgacctccag atgcaccttg gtgacgaggg gacaggtcgc
      841 atcgattacg cgcaggccgc ggtcgcgcgc atgctcccat tcgctgggcg aaacgccgtg
      901 ggcgctgaag atgacgcgct gggcttcggg cacatcgtcg agattttcga cgaaaatcgc
      961 gccttcctgc tccagcgcct ccaacacgaa gcggttgtgg acgatctgat ggcgcacata
     1021 aagcgggcgg ccatactcgc gcagcgcgcc gcgcaccgcc tcgaccgcgc gatcgacgcc
     1081 ggcgcaaaat ccgcgcggtt ccgccaggat gattttctgt atcgaagttt cgctcgggag
     1141 catattggcc gccttgttaa cgaatactgg ccgttcggtc aatcttgcca taatagcatt
     1201 aaatcccgcc atccgccgcg aattcgcgtc atccgtgcag gaactgccat cagcaatccg
     1261 atccggcaaa caccgccgca tgatgcagcg ccgcgatcgt tttcgcgtcg atgatctctc
     1321 cgcgccgcac catcgccagc gcatcttcga gcttcacccg ttcaacgcga atcacttcgt
     1381 cataatccag ttccccgttg gcgggggaaa gttcgcgcgc gagaaaaagc tcgatgcgct
     1441 cgtcgcagaa gctcggaatt gtgacgagcg accccagatg gtcccatcgc gcggccgcca
     1501 gtcccgcctc ctcgcgcaac tcccgtcgcg cgcattccag cgccgtctcg cttccgttgc
     1561 ggcatccggc gggaatttct cgcaaccacc cgccgatcgc atgccggtat tgcgtgagca
     1621 tcgcgatcgt tccctccgcg tcgagcgcaa cgatggcgct cgcccccgga tggcgaatga
     1681 cggccaactc gaccacggcg
//
LOCUS       DASZMC010000006         4737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3300071,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000006 DASZMC010000000
VERSION     DASZMC010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300071"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..540)
                     /locus_tag="VG274_00100"
     CDS             complement(142..540)
                     /locus_tag="VG274_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05076.1"
                     /translation="MTQISSKYRGIDVTANPRENGFCSRRLDAIAQKENRFPWHPGRG
                     IMKHRIHTIAFALAMAGALAACADSSDDSQAAALAQWKAYCGAQHKQFYWRDTEKARG
                     MLEQSVKVEGKCVGPGERGYEPPEPPDTSP"
     gene            643..900
                     /locus_tag="VG274_00105"
     CDS             643..900
                     /locus_tag="VG274_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWE05077.1"
                     /translation="MREMSVREANQNFSQVIAAAERGETIIVTKNGVPVARIAPQPLD
                     RREDPDWQAAHRALVQSLRTKPARGYRVGRITEDDKYGGGA"
     gene            897..1343
                     /locus_tag="VG274_00110"
     CDS             897..1343
                     /locus_tag="VG274_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWE05078.1"
                     /translation="MTRAALDSNILVYAELERESAKGRRAASLITRAARDGVIPVQAL
                     GEFLRVVQRRVPEAFDNAARQVAIYQDVFLTPATTNAVLGDAAALARDHRLRLWDCII
                     CAAALQSGAGIFLTEDMQDGRLIGRMRLLNPFLVSNTGAINAALRR"
     gene            1413..3299
                     /gene="mnmG"
                     /locus_tag="VG274_00115"
     CDS             1413..3299
                     /gene="mnmG"
                     /locus_tag="VG274_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HWE05079.1"
                     /translation="MSAYDVIVIGGGHAGCEAAAASARFGARTLLATHRRATIGEMSC
                     NPAIGGLGKGHLVREIDALDGVMGRVADEAGIQFRILNRRKGPAVRGPRAQADRKLYR
                     AAMQGALADTPNLEIAEFAVEDLVIRGGRAAGVRAEGGSELSCGAVILTTGTFLRGLI
                     HIGERKIPAGRMTEAPTVGLAETLYGLGFSMGRLKTGTPPRLDGASIDWAALEVQPGD
                     DPPMPFSFLTERITTPQIVCHITRTTRATHDIIRRNLDRAPIYSGQIESTGPRYCPSI
                     EDKVVRFAGRDGHQIFLEPEGIDDDTVYPNGISTSLPEEVQRDFLATIPGLERARMIR
                     PGYAIEYDYVDPRELVPSLETKRVAGLYLAGQINGTTGYEEAAAQGLMAGWNAARAAS
                     RSAPIILDRAEAYIGVLIDDLVTRGVTEPYRMFTSRAEYRLSLRADNADRRLTGFGVR
                     GGIVGSERARKFSAKIARLDAARRRLAELTLTPSQAGRAGIGVRLDGGRRNAHALLSL
                     PDVSVADLGRVWPELSGIDAEIVEQLENDAQYAVYLVRQAADIDAFRRDEALGLPHDL
                     DYGKVNGLSTEAAQKLSAIRPATLGQAGRIDGVTPAALTLVLAHARAKLRACGSSAAT
                     ADNR"
     gene            3488..4132
                     /gene="rsmG"
                     /locus_tag="VG274_00120"
     CDS             3488..4132
                     /gene="rsmG"
                     /locus_tag="VG274_00120"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HWE05080.1"
                     /translation="MGQAPSGHFGPAEFQAAANVSRETLARLKLFAALLEEWNARHNL
                     VSARSLTEVWHRHIWDSAQLAALVPPGAASLVDLGSGAGFPGLVLALLFRDRAGFRTV
                     LIEATRKKCDFLALAGQRLGLTVEIRNSRLEDAPREPFDIVAARACAPLPRLLVYAQA
                     FQGPNTLNLFLKGQSVGEELTEAHKSWRMQVREEPSRSDPSGVILAIRELRHAE"
     gene            4122..>4737
                     /locus_tag="VG274_00125"
     CDS             4122..>4737
                     /locus_tag="VG274_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWE05081.1"
                     /translation="MPSEPLSRGPRTLVVANQKGGVGKTTTAINLGTALAAIGQPTLL
                     IDIDPQGNASTGLGIARPDRKVTSYEVLTGEAGLADAIMPTRIPGLSLVPSTVDLSGA
                     ELELINLDRRNYRLKDALAIFIARESGRFAYVLIDCPPSLTLLTVNAMAAADAVVVPL
                     QCEFFALEGLSQLLKTIERVRVNLNPVLEIQGIVLTMFDKRNRLS"
BASE COUNT          797 a         1520 c         1556 g          864 t
ORIGIN      
        1 tccgtgcgcg tctgccttaa acccgttatt gtcatcgcac gcacgtcgcg tcccgcttct
       61 gcgcgcatga caaaggttgt tctgcaggaa tccggataat atgtataatg tccccttaac
      121 tctgccggat cccttgacgc atcaaggtga agtgtcgggc ggctccggcg gttcgtagcc
      181 gcgctcgccc gggccgacgc attttccttc gaccttgacg ctttgttcga gcattccccg
      241 cgccttttcc gtgtcgcgcc aatagaattg cttgtgctgc gccccgcaat acgccttcca
      301 ctgggcaagc gcggctgcct gggaatcgtc cgacgagtcg gcgcatgccg cgagcgcgcc
      361 cgccatggca agcgcaaatg caatcgtatg gatgcgatgt ttcatgatgc ccctccccgg
      421 atgccagggg aatcgattct ccttctgcgc gattgcgtca aggcgcctgg agcaaaatcc
      481 gttttcgcgt ggattggccg tcacgtcgat cccgcggtat ttgctcgaaa tttgcgtcac
      541 tttgcccctc cgccacttgt ctgccgcatg ccccgtctgc cgcatgccct tgccgccgcc
      601 gcgggcgcgc cgcagtgttg caacgtagaa caggagccgc aaatgcgcga aatgtcggtg
      661 cgcgaggcca accagaattt ctcgcaggtg atcgccgcgg ccgaacgcgg cgaaacgatc
      721 atcgtgacca agaacggcgt gcctgtcgca aggatcgctc cgcagccgct cgaccgaagg
      781 gaggatcccg attggcaggc cgcgcatcgg gcgctcgtac agtccctgcg cacaaagccg
      841 gccaggggct accgggtcgg acgaatcacc gaggatgaca agtatggcgg cggtgcatga
      901 cgcgagcggc gctggacagt aacattcttg tctatgcgga actggagcgc gaaagcgcga
      961 agggcaggcg cgccgcaagc cttatcacgc gggccgcgcg tgatggcgtc attcccgttc
     1021 aggcactggg agaatttctg cgggtcgtcc aacgccgtgt gccggaagcg ttcgacaatg
     1081 cagcgcgtca ggtggcgata tatcaggacg tgttcctgac tcccgccacg acgaacgccg
     1141 tgttggggga cgcagcagcc cttgcgagag atcatcggct tcggttgtgg gactgcatca
     1201 tctgcgcggc ggcgctgcaa tcgggcgccg ggattttcct cacggaagac atgcaggatg
     1261 gccggctgat cggccgcatg cggctgctca acccgtttct tgtctcgaac accggggcga
     1321 tcaacgcggc attgaggcga taaagcgcgc gtcccgattg tttcacgtga aacatgttgc
     1381 gcggtgcgca gatcgtgcct aaggcagcgc gcatgagcgc gtacgatgtc atcgtcatcg
     1441 gcggcggcca tgcgggctgc gaagcagcgg ccgcatcggc gcggttcggc gcccgcacgc
     1501 tgctggccac ccacaggcgg gcaacgatcg gcgaaatgtc gtgcaacccg gcgatcggag
     1561 ggctcggcaa gggtcatctg gtgcgggaga tcgatgcgct cgacggcgtg atgggccggg
     1621 tcgccgatga ggccggcatc cagttccgaa ttctgaaccg gcgcaaggga ccggcggtgc
     1681 ggggaccgcg cgcccaagcc gaccgcaagc tctaccgcgc ggcgatgcag ggggcgctcg
     1741 ccgacacgcc aaacctggag atcgcggaat ttgcggtgga ggatctcgtc atccggggtg
     1801 gcagggctgc gggagtccgc gcggaaggcg gcagcgagct ttcctgcggc gcggtcatcc
     1861 tcacgaccgg cactttcctg cgcggcctga tccatatcgg cgagcgcaaa atcccggctg
     1921 gccggatgac cgaggcgcca acggtcggcc tggcggagac cctgtatggg ctgggcttca
     1981 gcatgggccg gctcaagacc ggaacgccac cccggctcga tggcgcgagc atcgattggg
     2041 cggcgcttga ggttcagccg ggcgacgatc cgcccatgcc attctcgttc ctgacggaac
     2101 ggatcaccac cccgcagatc gtctgtcaca ttacccgcac cacacgcgcc acccacgaca
     2161 tcatccggcg caatctcgat cgggcgccca tctattccgg ccagatcgaa agcacggggc
     2221 cgcgttattg tccctccatc gaggacaagg tggtccgctt cgcggggcgc gacggccatc
     2281 agattttcct cgagcccgag ggcatcgacg acgacaccgt ctatccgaac ggcatctcga
     2341 cctcattgcc ggaagaggtg cagcgcgact tcctggcgac aattccgggg ctcgagcgcg
     2401 cgcgcatgat caggcccggc tatgcgatcg aatatgacta tgtcgatccg agggagcttg
     2461 tcccgtcgct ggagaccaag cgcgttgcgg ggctctatct ggcgggccag atcaatggga
     2521 cgacgggcta cgaggaagcg gccgcccaag gcctgatggc cggctggaac gccgcccgcg
     2581 ccgcgtcccg gtcggcgccg atcatcctgg atcgtgcgga ggcttacatc ggcgttctga
     2641 ttgacgatct cgtcaccagg ggggtgaccg agccctaccg catgttcacc tcgcgcgcgg
     2701 agtatcggct cagtctgcgt gcggacaatg cggatcgccg cctgacgggt ttcggggttc
     2761 ggggcgggat cgtgggcagc gaacgggcgc ggaaattctc cgcgaagatc gcccggctgg
     2821 acgccgcccg gcgtcgcctg gccgagctca ccctcacgcc gagccaggcc gggcgggcgg
     2881 ggattggcgt gcggctcgat ggcggccggc gcaatgcgca cgcgttgctc agcctgcccg
     2941 atgtgagcgt tgccgatctg gggcgggtgt ggccggagct gtccggcatc gacgccgaaa
     3001 ttgtcgagca gcttgagaac gacgcgcaat atgccgtcta tctcgtgcgc caggccgccg
     3061 atatcgatgc cttccgccgc gacgaggcct tggggcttcc ccacgatctc gactacggca
     3121 aagtcaatgg gctgtcgacg gaggcggcgc agaagctgag cgccatccgt ccggcgacgc
     3181 tcggccaggc cggccgcatc gacggtgtca ccccggccgc gctgacgctg gtgctagcgc
     3241 atgcgagggc gaagttgcgc gcatgcggtt catcagccgc gacggccgac aaccgctgat
     3301 gttgcagcac ccctcaccca tgcttgagcg gctgccagac atcgtctcta gcccctctcc
     3361 cgcaagggga gagggcatcc cccgcgctgc ttttccgcca tccatgcaag actccgaatt
     3421 cccctcgccc cttgtgggag agggatgcgg gtacgggcgg cttattctgc cgctgcgtgg
     3481 gtgaggggtg gggcaagcac caagcggtca tttcggtccc gcggagtttc aggcggcggc
     3541 aaatgtttca cgtgaaacat tggcgcgcct gaagctgttc gcggccctgc tggaggaatg
     3601 gaacgcgcgt cacaatctgg tctcggcgcg atcgctgacc gaggtctggc accgccatat
     3661 ctgggacagc gcgcagcttg cggcgttggt gccgccaggc gcggcgagcc ttgtcgatct
     3721 gggcagcggc gccggctttc ccggactggt gctcgcgctt cttttccgcg atcgggctgg
     3781 cttccgcact gtgctcatcg aggccacccg caagaaatgc gactttctcg ccctggccgg
     3841 acaaagactt ggactgaccg tcgagatccg caattcgcgt ctggaagacg cgccccgcga
     3901 gcccttcgat atcgtggccg cccgggcttg tgcgccgctg ccgcggctgc ttgtctatgc
     3961 acaggctttt cagggcccga acacgctgaa tctctttctc aagggtcaat ctgtagggga
     4021 agaattgacc gaggcccata aatcttggag gatgcaggtg agggaggagc ccagccgctc
     4081 cgatccttcc ggcgtcatcc tcgcgatccg ggagcttcgc catgccgagt gagccgctgt
     4141 cgcgaggtcc ccgaacgctc gttgtcgcca accagaaggg cggggtcggg aagacgacca
     4201 cggcgatcaa tctcggaacg gcgcttgccg ccattggtca gccgacactg ctgatcgata
     4261 tcgatccgca gggcaatgcg tcgacgggtc tgggcatcgc gcggccggac cgcaaggtca
     4321 cctcctatga ggttctgacg ggggaggccg ggctcgccga tgcgatcatg ccgacacgca
     4381 ttcccggcct ctctctcgtc ccatcgaccg tcgatctttc gggggccgaa ctcgaactga
     4441 tcaacctcga tcggcgcaat taccggttga aggacgcact ggccatcttc attgcgcgcg
     4501 aatccgggcg cttcgcctat gtgctcatcg attgtcctcc gtcgctgacc cttctgacgg
     4561 tcaatgcgat ggccgcggcc gacgccgtgg tcgtgccgct gcagtgcgag ttcttcgcgc
     4621 tcgaaggcct gagccagctt ttgaagacca tcgagcgcgt acgcgtcaat ctcaatcccg
     4681 tcctggagat tcaggggatc gtgctcacca tgttcgacaa gcggaaccgg ctttccg
//
LOCUS       DASZMC010000007         4740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3301359,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000007 DASZMC010000000
VERSION     DASZMC010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3301359"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..1162
                     /locus_tag="VG274_00130"
     CDS             404..1162
                     /locus_tag="VG274_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05082.1"
                     /translation="MIGKLALAALAAAAFALPAMAAKETIPANIAAAVADPARPATDK
                     DRDANRKPAECLAFAGVKPGDRVADLIPGTGYFTRLFSKAVGAKGYVYSYIPSDIDAI
                     YKKHNVTIPPPADPNFPNISYIHAPIAKFVTPEKLDLVWTSQNYHDLHDKFFGPPDIA
                     EVNRAIYVSLKPGGIFIVLDHAAEPGSGLRDTDTLHRIDVAAVKKEVEAAGFKLVAES
                     AILHNPADTHKVAVFDPSIRGKTDQFILKFRKPR"
     gene            1343..2389
                     /locus_tag="VG274_00135"
     CDS             1343..2389
                     /locus_tag="VG274_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05083.1"
                     /translation="MSGGKGDRVAMARNHDGRLEAFYCGPDNSIFHQRQVSPGGRWTP
                     PRATGNSANELALASNVDGRLELFWVDARFSLFHMRQKDAGRAWSEHKQVPGRGRHIA
                     LERNLDGRLELAYVGLDSRIYCDRQVFPGRSWSGPQALPGIARKLAPARNKDGRIELF
                     YVGADGGIYHNWQQSPGKAWDGSAVRPGKATQIAAACNADGRLEIFWIGPDSAVYHSW
                     QIAPGKGWAGSATLQVAAKNIAAFTSADGRLEVFYVGTDSRIRYLRQLSPGKNWSAPS
                     VVAAHAAHIAAAQNADGRLELFYIGTDAAAHHLRQVSPGGAWQDVEVDADADVHADVH
                     ADVHAPAPREALQA"
     gene            complement(2501..3277)
                     /locus_tag="VG274_00140"
     CDS             complement(2501..3277)
                     /locus_tag="VG274_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05084.1"
                     /translation="MSTEFQGLYLCVRVPKSGSASLMRGLEQAFGCQQIFYLPNTADL
                     DGQVSRLQRLRFWRARFQNLFRHYRTFSMRAPFERINRDAAPGDLVMGGHIDFRTAQA
                     AIARPLKIITLLREPVVRARSEYDYLRRKYLRKPRLNRFDASILHKAAGRYDFDSFLD
                     FLSEHQSVYGDIACRYVGWDGQEDLAGFFTRYIFHSGVLERSDEFAHAIAEKLGRPFS
                     LPHENRDTKERSPVTGAQRAKLERIYARDILLHEWARTHR"
     gene            3373..>4740
                     /gene="ahcY"
                     /locus_tag="VG274_00145"
     CDS             3373..>4740
                     /gene="ahcY"
                     /locus_tag="VG274_00145"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWE05085.1"
                     /translation="MAQDYAVKDINLADWGRKEIDIAETEMPGLMATREEFGESKPLK
                     GARVAGSLHMTIQTAVLIETLKGLGADVRWASCNIYSTQDHAAAAIAAGGTPVFAIKG
                     ESLEDYWEYSHRIFEWADGGVPNMILDDGGDATLLVHLGARAEKGDAGFLDKPQNEEE
                     EVLYAAILRRLKHKRGWYTELAQKIRGVTEETTTGVHRLYQMEKDGKLLFPAINVNDS
                     VTKSKFDNLYGCRESLVDGIRRGTDVMMSGKVAMVAGFGDVGKGSAASLRQAGCRVMI
                     SEVDPICALQAAMEGYEVVTMDDAAPRADIFVTATGNVDVITIDHMRAMKHRAIVCNI
                     GHFDSEIQVAALKNFKWHNVKPQVDEVEFANGRRIILLSEGRLVNLGNAMGHPSFVMS
                     ASFTNQTLAQIELFANPGRYEKKVYTLPKHLDEKVARLHLEKIGVKLTTLSNKQSDYI
                     GVPQTG"
BASE COUNT          988 a         1448 c         1464 g          840 t
ORIGIN      
        1 agcatcaggt ggctagtgtc tcatggttcg acggctcacc atgaggagga acccctcacc
       61 ctgagcccgt cgaagggtgg cgcaaaacgt ccgggacgcc gcgattgctc acggcgtccc
      121 gggcattcca tgcgattcaa cggttcagag ctctgcgcag gcgtagcagt tgatttccat
      181 gccaacacag atctcgacga ccttcggtgt cgtccacttc atggtgctct cctggcaact
      241 aacggtgcgt gacgctaaca cggtttcgcc gaaccggaaa cccctataag agaaaccggc
      301 tccgggcgcg ggggaagccc gccgcgacgc gacttgcaaa tgggcgcccg gaagacttga
      361 atcggcgcgc gccggcatgt aagcccggcg aggagggact tccgtgatcg gaaaacttgc
      421 cttggcagcg ctggccgccg ccgcgtttgc cctgcccgcc atggccgcga aggaaacgat
      481 ccccgccaat atcgccgcgg cggtcgccga cccggcgcgc ccggccaccg acaaggatcg
      541 cgacgcaaac cgcaagcccg ccgagtgtct cgccttcgcc ggcgtcaagc ccggcgaccg
      601 cgtcgccgac ctcattccgg gcaccggcta tttcacgcgg ctcttttcga aggccgtcgg
      661 agcgaaaggt tatgtctatt cgtatattcc ttccgacatc gacgcgatct acaagaaaca
      721 caatgtgacg atccctccgc ccgcggatcc caacttcccc aacatctcct acattcacgc
      781 gccgatcgcg aaattcgtca cgccggaaaa gctcgatctc gtctggacgt cgcagaacta
      841 tcacgacctg cacgacaagt tcttcggccc gcccgacatc gccgaggtca acagggcgat
      901 ctacgtttcg ctgaagccgg gcggcatctt catcgtgctc gaccacgcgg cggaacccgg
      961 ttcgggcctg cgcgacaccg acacgctgca tcgcatcgac gtggcggcgg tgaagaagga
     1021 agtggaagcg gccggattca aactcgtcgc cgagagcgcg atcctgcata atcccgccga
     1081 tacgcataag gtagcagtgt tcgatccctc catccgcggc aagaccgacc agttcatcct
     1141 gaaattccgc aaaccgcgat gaaccacatg attgctattc gatgccgagc tggaccgccc
     1201 gcagcgttgc gatagcggca agcgacgatt tctggcctcc agatatcaac ttgcgcgaac
     1261 gaatacggcg ctcgaaacga actgtcgtat gcctgtcacg cccggcggcc aagctggggg
     1321 acaaacatgg tgggtgaaat acatgagtgg tggaaagggt gaccgcgtcg ccatggcgcg
     1381 caatcatgac ggacgcttgg aggcgttcta ctgcggacca gacaattcga tcttccatca
     1441 gaggcaggtt tctcccggcg gccgctggac cccgccgcgc gcaaccggca attccgccaa
     1501 cgagcttgcg cttgcaagca atgtggacgg tcgcctggaa ctcttctggg tcgacgccag
     1561 attttcactt tttcacatgc gccagaagga tgctggaaga gcctggagcg aacacaaaca
     1621 ggttcccgga agaggacggc atatcgccct cgagcgcaat ttggacgggc ggctcgaact
     1681 tgcttacgtg ggcctcgatt cccgcatcta ctgcgacagg caggtgtttc cgggaagatc
     1741 ctggagcggc ccgcaggccc ttccgggtat tgcgcgcaag cttgcgccgg cccgcaacaa
     1801 ggacggacgg atcgagcttt tctatgtcgg agcggacggc ggcatttatc acaactggca
     1861 acagtcgcca ggcaaggcat gggacggctc cgctgtccgt cccggcaagg ccacgcaaat
     1921 cgccgcagcg tgcaatgcag acggaagact cgagatattc tggattggac cggattccgc
     1981 cgtctatcat tcttggcaga ttgcgcccgg gaagggctgg gcaggttcgg cgacgctgca
     2041 ggtggccgcc aagaatattg cagcgtttac gagtgccgac ggtcgtcttg aagtcttcta
     2101 cgtcggcacg gactcccgca ttcgctacct gcggcaatta tcgcctggga agaactggag
     2161 cgctccgtcg gtggtcgccg cccacgcagc gcacatcgcg gcggcgcaaa acgccgacgg
     2221 ccggctagaa ctcttctaca tcgggaccga cgcggcggcg catcatctcc ggcaggtctc
     2281 tccgggcgga gcgtggcaag acgtcgaagt tgacgccgat gccgacgtcc atgccgacgt
     2341 ccatgccgac gtccatgcac ccgctccccg ggaagccctc caagcgtagc gcccgaaagc
     2401 ataatcaaaa cggccagaaa tccggaccac ggcaggtatc ggcattcacc atggcccgct
     2461 atcgcaacgg caggattcga tcgggcgcgc tgccaaacgg ctatcgatgc gtcctcgccc
     2521 actcatggag caaaatgtcc cgcgcgtaga tgcgttcgag tttcgcgcgt tgcgcgccag
     2581 taacagggct tcgttccttg gtgtcgcggt tttcatgagg aagcgagaac ggccggccga
     2641 gcttttcggc gatggcatgc gcgaattcgt cgctgcgttc gagcacgccg ctatgaaaga
     2701 tatagcgcgt aaagaatccg gccaagtctt cctgcccgtc ccagccaaca taacggcatg
     2761 cgatgtcgcc atagacgctc tgatgctcag aaagaaaatc gaggaaggaa tcgaaatcgt
     2821 agcggccggc ggctttgtgc agaatgctgg cgtcgaagcg gttgaggcgg ggcttgcgca
     2881 ggtattttcg gcgcagataa tcatattccg agcgtgcgcg gacgaccggc tcgcgcagca
     2941 gagtgatgat cttgagcggt cgcgcgatcg ccgcttgcgc ggtccggaaa tcgatatggc
     3001 cacccatgac gaggtcaccc ggcgccgcgt cgcggttgat gcgttcgaac ggtgcgcgca
     3061 tgctgaacgt ccgatagtgg cgaaagagat tctggaagcg ggcgcgccag aagcgcaggc
     3121 gctgaaggcg cgacacctgc ccatcgagat cggccgtgtt cggcagatag aatatctgtt
     3181 ggcagccgaa cgcctgctca aggccccgca tcaggctcgc gctgccgctc ttgggcacgc
     3241 gaacgcagag atacagccct tggaactcgg tgctcatccg gtgacaaata acgatatgta
     3301 catttcttta tatccttgtt gaaaccgacc caacaggctg gtatatccgc ccgcccccac
     3361 cggagagccc ccatggccca ggactatgcc gtcaaagaca tcaaccttgc agactggggg
     3421 cgcaaggaga tcgacatcgc cgagaccgaa atgcccgggc tgatggcgac acgggaggag
     3481 ttcggcgaat cgaagccgct caaaggcgcg cgcgttgcgg gctctctgca catgacgatc
     3541 cagaccgcgg tgctgatcga gacgctgaaa gggctcggcg ccgatgtgcg ctgggcctcg
     3601 tgcaacattt actcgacgca ggaccatgcc gcggcggcga tcgcggccgg cgggactccg
     3661 gttttcgcca tcaagggcga gagtctcgag gattattggg aatactcgca ccgcattttc
     3721 gagtgggcgg atggcggagt gcccaacatg atcctcgacg atggcggcga cgccacgctg
     3781 ctcgtgcatc tgggcgcgcg cgcggagaaa ggcgatgccg gttttctcga caagccgcag
     3841 aacgaggagg aggaagttct ctatgcggcg atcctgcgcc ggctgaagca caagcgcggc
     3901 tggtacaccg agcttgcgca aaagatccgc ggcgtcacgg aagagacgac gaccggcgtg
     3961 caccgcctct accagatgga gaaggacggc aagctgctgt tccccgccat taacgtgaac
     4021 gatagcgtca ccaaatcgaa attcgacaat ctctatggct gccgcgaaag cctggtggac
     4081 ggcatccgcc gtggcaccga cgtgatgatg tccggcaagg tggcgatggt cgcgggcttt
     4141 ggcgacgtgg gcaaaggctc ggccgcgtcg ctgcgccagg ccggctgccg ggtgatgatc
     4201 tcggaagtgg accccatctg tgcgctgcag gcggcgatgg aaggctatga agtggtgact
     4261 atggacgatg cggcgccccg cgccgatatt ttcgtcaccg cgacgggcaa tgtcgacgtc
     4321 atcacgatcg atcatatgcg ggcgatgaag caccgcgcca tcgtctgcaa tatcggccat
     4381 ttcgattccg agatccaggt tgcagcgctc aagaacttta aatggcacaa tgtgaagccg
     4441 caggtggacg aggtcgagtt cgccaatggc aggcgcatca tcctgctttc ggaagggcgg
     4501 ctggtgaatc tcggcaatgc gatggggcat ccttccttcg tgatgagcgc gtcattcacc
     4561 aaccagacgc tggcgcagat cgagctgttc gcgaatccgg gcagatacga aaagaaggtc
     4621 tatacgctgc ccaagcacct cgacgagaag gtcgcgcgcc ttcacctcga gaagatcggc
     4681 gtgaagctga ccacactctc caacaagcaa tccgattata tcggcgtgcc gcagacggga
//
LOCUS       DASZMC010000008         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2477265,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000008 DASZMC010000000
VERSION     DASZMC010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477265"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..485)
                     /locus_tag="VG274_00150"
     CDS             complement(36..485)
                     /locus_tag="VG274_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWE05086.1"
                     /translation="MISVLLLPEPLRAHLVKEARDAFPRECCGLVEGTVSGAQAGATA
                     LHATRNLAVEPDRFEIDPAVQFALLRGLRGTVRQIIGCYHSHPNERAEPSGRDRAAAS
                     EDGFLWLIAALGARGRPRIAGFVSTGFAFSPIRIEAASLDRVVAPPV"
     gene            complement(482..841)
                     /locus_tag="VG274_00155"
     CDS             complement(482..841)
                     /locus_tag="VG274_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05087.1"
                     /translation="MADLSDSGTTPPVQPAKQTAAYRAALAAVILLSLLIAIALGTLV
                     IGFAMHAGGRPHDSGATGAVQLVLAPDMKIVSADFSGDRLVLRLHGPAGDQIDIIDTG
                     TGRLVARVRSAAPPPRK"
     gene            867..>1757
                     /locus_tag="VG274_00160"
     CDS             867..>1757
                     /locus_tag="VG274_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWE05088.1"
                     /translation="MRRAVVEPAQSGARLDRFLAAALPDLSRSRLQQLIAQGAVTRLG
                     LTIGDPNQRVKPEETYEVRVPPPAPAAPAGQDIPLNVIHEDDDLIVIDKPAGLVVHPA
                     AGNPDGTLVNALIAHCGTSLLGIGGEARPGIVHRLDKDTSGLIVAAKNGRAMHSLAKQ
                     FAHRTIERAYDAVAWGSPRAGEGVVEGDIGRNPFDRKRMAVLRGGGKPARTLYRLQER
                     YGNPQRPFASLIECRLETGRTHQIRVHLAHIGHPLIGDSVYGRARSVPRAKTPAAAEA
                     FAAAAGFPRQALHARVLGFKH"
BASE COUNT          326 a          581 c          592 g          258 t
ORIGIN      
        1 ctagacgatg ggagctgtcc cgcggaattg gggcgttata ccggcggcgc gacaacgcga
       61 tcaagcgaag ccgcttcgat ccgaatgggc gagaacgcga aaccggtgct cacaaaaccg
      121 gcgattcgag gccgcccgcg agccccgaga gccgcaatca gccatagaaa tccatcttcg
      181 gaagccgcgg cacgatcgcg cccggagggc tcggcgcgtt cattgggatg tgaatgataa
      241 cagccgatga tctgccgcac cgtgccgcgc aagccgcgca gcagcgcgaa ttggactgcg
      301 ggatcgattt cgaagcgatc gggttcgacg gcgaggtttc gtgtggcgtg cagtgcggtc
      361 gcccccgcct gcgcgccgga caccgtgccc tcgacgaggc cgcagcattc gcgggggaat
      421 gcgtcgcgtg cctctttcac gagatgcgcg cgcagaggtt ccggcaaaag aaggaccgag
      481 atcatttgcg cggtggaggt gctgcagacc gcacacgcgc aacaaggcgg ccggttcccg
      541 tgtcgatgat gtcgatctgg tcgcccgccg gcccgtgcag ccgaagcaca agtctgtcgc
      601 cggaaaaatc cgcggataca atcttcatat ccggtgcaag aacgagctga acggcgccgg
      661 tcgcccccga atcatgcggc cggccgccag cgtgcatcgc gaatccaatc acgagcgtgc
      721 cgagcgcaat ggcgatgaga aggctgagca ggatgacggc cgcaagcgcg gcgcgatagg
      781 ctgccgtctg cttcgcgggc tgaacgggag gcgtggtgcc ggagtccgac aaatctgcca
      841 ctgcacgtgc ctccgggaac gagggagtgc gacgagccgt cgttgagccc gcacaatcgg
      901 gcgcgcgcct cgaccgtttc ctggccgccg ccctgccgga tctcagccgc tcccggctgc
      961 agcaattgat cgcgcaaggc gccgtcacgc ggcttggcct gacgatagga gacccaaatc
     1021 agagggtcaa acctgaagag acctatgagg ttcgcgttcc tccgcctgcg cccgctgcgc
     1081 ccgccgggca ggacattccc ctgaacgtga tccatgagga cgacgatctg atcgtgatcg
     1141 acaaaccggc cggcctcgtg gttcatcccg ccgccggcaa ccccgacggc acgctcgtca
     1201 acgccctcat tgcgcattgc ggcacgtcgc tcctgggcat cggcggcgaa gcccggcccg
     1261 gaatcgtgca ccggctggac aaggacacga gcggcctcat cgtcgcggcg aagaacggcc
     1321 gcgccatgca cagcctggcc aagcagtttg cccaccgcac catcgagcgc gcctatgatg
     1381 ctgtcgcatg gggcagtccc cgcgccggag agggcgtggt cgagggcgac atcggacgca
     1441 atcccttcga ccgcaagcgg atggccgtgt tgcgcggcgg gggcaagccg gcgcggacgc
     1501 tttaccgcct gcaggagaga tacggaaacc cgcaaaggcc cttcgcgagc ctgatcgaat
     1561 gccgactgga aacaggccgg acgcatcaaa tccgcgtgca tctggcccat atcggccatc
     1621 cgctgatcgg cgactccgtc tatggccgcg cgcgcagcgt gccgcgcgcc aagacgcccg
     1681 cagcggccga ggcgttcgcg gcggctgcgg gtttcccgcg gcaggcgttg cacgcgcgcg
     1741 tgctggggtt caagcat
//
LOCUS       DASZMC010000009         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_552148,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000009 DASZMC010000000
VERSION     DASZMC010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552148"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..971)
                     /locus_tag="VG274_00165"
     CDS             complement(117..971)
                     /locus_tag="VG274_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWE05089.1"
                     /translation="MQRLILPMIALAATLHAQPQSSTLPVETKVWVATKLYSGIQMYF
                     AHAEGAPEFDLDRDFQQHLKAAFAAPDRRTFDLASMAFMGKLRNGHSFFYDEWLFKNY
                     GQNLGFTLLPMTDGWVVTFSRLPDLNPGDIVVSVGGKPIEQFYAEYDGMIEGSSESTR
                     RRRFLWQTYLWPAKFELGLAGGKQVAIDRLQQTLQRTRGFQTPQGEPKLPAGIAYLRL
                     SSFEDPKIELSAVTRVKELQSANAIIFDVRGNGGGSTPSQLIAALLNRPWRDFRFTTP
                     FSIAHAGA"
     gene            complement(1052..2014)
                     /locus_tag="VG274_00170"
     CDS             complement(1052..2014)
                     /locus_tag="VG274_00170"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HWE05090.1"
                     /translation="MTRSLSTVFAASLALAAIASAQSATPPNHGPDAAQFVTSDIPNF
                     WRVFDHATLQNASSLLQTEYIDPGSVGLHDFLQSRIQNGRALAATVAARPRYYAAIRE
                     NTLAVDRDPAIKQAIRASFHRLKEIYPDATFPNVYFVIGRMNSAGTVSSHGLLIGVEM
                     NARDDNTPVDELNAWESAVTGRAANLPHIVAHELIHIQEPDEKPKPTLLEAAIREGAA
                     DFLGELISGAIINNAQRTYGDAHEQVLWEEFKKAMTGADVSNWLFQGDRSQGRPADLG
                     YYIGYRICEAYYRRTPDKQAAVAHILRMSDAPAFLAESGYTGAH"
     gene            2116..2874
                     /locus_tag="VG274_00175"
     CDS             2116..2874
                     /locus_tag="VG274_00175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE05091.1"
                     /translation="MAKNGKFAVVTGAGSGIGRAVALGLQGAGYTVALAGRRKSELDA
                     TAAQAAPAGGAMVAIPTDVTDAAAVAALFVRVKQEFGRLDLLFNNAGKGAPGVPMEEL
                     TLEDWNAVVAVNLTGAFLCAQHAVRMMKKQTPRGGRIINNGSISAHTPRVNSAPYTAT
                     KHAITGLTKSISLDGRGFDICCGQIDIGNAETPMTARMPAGVAQANGTVAPEPRMDVR
                     HAAEAVVFMASLPLDANVPFLTVMANGMPFMGRG"
BASE COUNT          550 a          743 c         1061 g          563 t
ORIGIN      
        1 gggatgcagc gggccgggcg tgaggattgg gtgttcttga attcttcgta ggcatcgaga
       61 tagcccttgt cgtactcatt ctgctccagg gtcggaacca tgaggcgcac ctggttctac
      121 gcgcctgcat gggcgatcga gaatggggtc gtgaagcgaa agtcacgcca cgggcgattg
      181 agcagagctg caatcaactg gctcggtgtg gatccgccgc cattcccgcg cacgtcgaag
      241 atgatcgcgt tggcggactg cagctccttc acgcgcgtaa cagccgacag ttcgatcttc
      301 gggtcctcaa acgatgagag cctcaggtag gcgatgccgg cggggagctt cggttcccct
      361 tggggagttt gaaatccgcg ggtgcgctgc agcgtctgtt ggagtcgatc gatggcgact
      421 tgtttgccgc ccgcaagccc gagctcgaac ttagcgggcc aaaggtatgt ctgccacaaa
      481 aagcggcgcc ggcgcgtgct ctccgatgat ccttcgatca tgccgtcgta ctcggcgtag
      541 aactgctcga taggtttgcc gccgacggag acaacgatgt cgcccgggtt cagatcgggc
      601 aggcggctga aggtgacaac ccagccatca gtcatgggaa gcaacgtgaa gccgagattc
      661 tgcccgtagt tcttgaacag ccactcgtcg tagaagaagg agtggccgtt gcgaagcttg
      721 cccatgaacg ccatggaggc gaggtcgaat gtacggcggt cgggtgcggc aaaagcagct
      781 ttgaggtgct gctggaagtc gcgatcgaga tcgaattccg gagcgccttc ggcgtgcgcg
      841 aagtacatct ggatgcccga atagagtttc gttgccaccc agaccttggt ctcgaccggt
      901 agggtagagc tttggggttg cgcgtgaagt gtggcggcca gtgcgatcat aggcaagatc
      961 aagcgctgca tcgaacttat gttgacatag gacgctacgt cgccgcgctt cgttgaatag
     1021 taggggcggt acgggcattc gcgccggcgc gttagtgagc gcccgtgtat ccactctcgg
     1081 cgaggaaggc cggggcgtca gacatgcgca ggatgtgcgc gacggcggcc tgcttgtcgg
     1141 gcgtgcggcg gtaataggcc tcgcaaatgc ggtagccgat atagtaaccg aggtcggcgg
     1201 gacggccctg cgagcggtcg ccttgaaaga gccaattgct aacgtcggca ccggtcatcg
     1261 cctttttaaa ttcttcccag aggacctgtt catgagcgtc gccgtaggtg cgctgggcgt
     1321 tattgatgat ggcgccggag atcagctcgc cgagaaaatc cgcggcgcct tctctgatgg
     1381 cagcttcgag gagggttggc ttcggtttct cgtcgggctc ctggatgtga atcagttcgt
     1441 gcgcgactat gtggggcaag ttggcagccc ggccggtgac ggcgctctcc caagcgttta
     1501 actcatccac gggcgtgttg tcgtcgcgcg cgttcatctc tacgccgatc aggagaccgt
     1561 gggaggaaac ggttcctgcg gaattcatcc ggccgatgac gaaatagacg ttggggaacg
     1621 tggcgtcggg atagatttct ttgagccgat gaaagctggc gcggatggct tgcttgatgg
     1681 cgggatcgcg atcgacggcg agcgtgtttt cgcgtatggc ggcgtagtaa cgcgggcgcg
     1741 cagcgacggt ggcggccagg gcgcgcccat tttggatgcg gctctgcagg aaatcgtgaa
     1801 ggccgacgct gccgggatcg atgtactcgg tttgcaagag cgaggaggcg ttttgtagag
     1861 tggcgtgatc gaaaacgcgc cagaagttgg gtatgtcgct ggtaacgaac tgcgcggcgt
     1921 cgggaccgtg attgggcggg gtggcggact gcgcggatgc aatagcggcg agggcgagag
     1981 aggccgcaaa taccgtagac aagctgcgcg tcatagagga tcgcttataa gtacggccga
     2041 tttcgaaaat agttacgcgg agtggtgagc ggacagaccg cgttggtgaa atcctgccct
     2101 tgctatgctg agccgatggc gaagaacgga aaatttgcgg ttgtaaccgg agcgggcagc
     2161 ggcattggac gcgcggtagc gctcgggctg caaggcgcgg gctatacggt ggcgctggca
     2221 ggcaggcgga agagcgaatt ggacgcgaca gcggcgcagg ccgcgccggc cggcggcgcg
     2281 atggtggcga ttccgacgga cgtgacggac gcggcggcgg tggcggccct gttcgtgcgc
     2341 gtaaagcagg agtttggacg gctggacctg ctgttcaaca acgcggggaa gggcgcgccg
     2401 ggcgttccga tggaggaact gacgctggag gattggaacg ccgtggtggc ggtgaatttg
     2461 acgggcgcgt ttctgtgtgc acagcatgcg gtgcggatga tgaagaagca gacgccgcgg
     2521 ggcgggcgca tcatcaacaa cgggtcgatt tcggcgcata cgccgcgggt gaattcggcc
     2581 ccgtacacgg cgacgaagca tgcaattacg gggttgacca agtcgatttc gctcgacggg
     2641 cgcgggtttg atatctgctg cgggcagatc gacatcggaa acgcagaaac gccaatgacg
     2701 gcgcgcatgc cggcgggcgt ggcgcaggcg aatgggacag tggcgccgga gccgcgcatg
     2761 gatgtgcggc acgcggcgga ggcagtggtg ttcatggcga gcctgccact ggatgcgaac
     2821 gtcccgtttc tgactgtgat ggcgaacggg atgccgttta tggggcgagg ctagcctgca
     2881 tttctcacac aggttcgtcg cgaggcgcgt gagaagg
//
LOCUS       DASZMC010000010         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_279035,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000010 DASZMC010000000
VERSION     DASZMC010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279035"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..>1609
                     /locus_tag="VG274_00180"
     CDS             108..>1609
                     /locus_tag="VG274_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HWE05092.1"
                     /translation="METRTAFWTALTRFERNKILPDIAIRNTVGFIGAVVLGTVFGSP
                     STGVVTGIGALNVSFSDSRDPYILRARRMSLAALMCGIAVTAGSLSGHSNILAVIVAG
                     LWAFSAGMMVALGSPAGDLGVTTLVTVVVFAARPQPPLIAIESGLNAMAGAFLQILLS
                     IAPWPWRRYEPEKRIVNSLYRALAGMARSPGVPSSAPPLTGQISDAREALGSLTSDHG
                     LEAERLVFLLNQAERIRLSILTLGRLSRRLARAPEGAEAAAALTHVLETAADALDGRL
                     DAFTHAARQFRKRNWNFDSKFVRALIRDAIQQVDALGGQIRAATGTVPAAYDPQEARE
                     PWRLRFTGRLARLEANLSLDSTVFRHALRLALCLGIGDAAGRALSLQRTYWIPMTIAI
                     VLKPEFTATFTRGFLRIAGTLAGLLLATVLFRFVHTGSATDIVLMGVFFFLLRWVGPA
                     NYGVFVTALSAMVVLLIATTGVSPGEVIAARAINTVIGGAIAMAAYALWPT"
BASE COUNT          239 a          608 c          448 g          314 t
ORIGIN      
        1 ccgcgggcaa aaacgtcatc cgggatggaa accgctgtct tcatatcgaa agtatgacgg
       61 agcgagaacc tttacttccg cctccaggag attatgaata gcacgccgtg gaaacccgca
      121 ccgcattctg gaccgccctg acgcgtttcg agcgtaacaa gatccttcct gacatcgcca
      181 tccgcaacac cgttggtttc atcggcgccg tagtcctcgg cacagtcttc ggctcgccga
      241 gcaccggagt cgtcaccggc ataggcgccc tcaacgtttc tttttccgat agccgcgatc
      301 cgtacatcct ccgcgcccgc cgcatgtcgc tcgcggcgct gatgtgcggc atcgcggtca
      361 ccgccgggtc gctctccgga cattcgaata tcctcgctgt aatcgtggcg ggcctgtggg
      421 cgttttccgc cggtatgatg gtcgcgctgg gatctccagc gggcgacctc ggtgtcacca
      481 ctctcgtgac agtcgtggtc ttcgccgcgc ggccccagcc gcctctcatc gcgatcgagt
      541 ccggtctcaa tgccatggcg ggcgcgtttc tgcagatcct gctctctatc gcgccatggc
      601 cctggcgccg ttacgaaccg gagaagcgca tcgtgaactc gctctaccgc gcgctcgccg
      661 ggatggcgcg cagtcccggc gttccgtcga gtgcgccgcc gctcaccgga cagatctccg
      721 atgcgcgcga agcactcggc tccctcacat cggaccacgg cctcgaagcc gagcgtctcg
      781 tcttccttct gaatcaggcc gaacgcattc gtctctcgat cctcaccctc ggccgcctct
      841 cgcgccgtct ggcccgcgct cccgaaggcg ccgaagccgc cgccgccctc acgcacgtgc
      901 tcgaaaccgc ggccgatgcc ctcgacggcc ggctcgatgc cttcacccat gcggcccgcc
      961 agttccgcaa acgcaactgg aacttcgatt cgaaattcgt ccgcgccctc atccgcgatg
     1021 cgatccagca ggtcgacgcg ctgggcggcc agatccgcgc cgcaacggga acggtccccg
     1081 ccgcctacga tccgcaggaa gcgcgcgaac cctggcggct ccgcttcacc gggcgcctcg
     1141 cccgcctcga agccaacctc tcgctcgatt caacggtctt ccgccacgcg ctccggctgg
     1201 ctctgtgtct cggtatcggc gacgccgccg gccgcgctct ctcgcttcag cgcacctact
     1261 ggatccccat gacgatcgcg attgtcctga agcccgagtt caccgccacc ttcacgcgcg
     1321 gtttcctccg tatcgccgga accctggccg gactcctgct cgccaccgtc ctttttcgtt
     1381 tcgtgcacac cggcagcgcc accgacatcg tgctgatggg cgtgttcttc tttctcctgc
     1441 gctgggtcgg cccagccaat tacggtgtct tcgtcactgc cctcagcgca atggttgtgc
     1501 tgctgatcgc caccaccggc gtatcgccgg gtgaagtcat cgccgcccgc gccatcaaca
     1561 ctgttatcgg cggcgccatc gcgatggcgg cctacgcgct ctggcccac
//
LOCUS       DASZMC010000011         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1929126,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000011 DASZMC010000000
VERSION     DASZMC010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929126"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /gene="putA"
                     /locus_tag="VG274_00185"
     CDS             <1..1967
                     /gene="putA"
                     /locus_tag="VG274_00185"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HWE05093.1"
                     /translation="EGIFPQFATHNAHTLSAVQVLAGNNPDFEFQRLHGMGEALYELY
                     REMKQPGRAGPATRIYAPVGTHEDLLAYLVRRLLENGANTSFVNRLADDESPVAEIIA
                     DPVEQLAASSPRRSSRIPKPIDLLADRRNSTGFLWSDPEIGAPIIAQMSKALEPPQRA
                     APLIGGKVRTGVPREVRNPADRCRVVGEVIEAAEADAIDALALGHRAQEAWDKRGGDA
                     RARILEQAAELFEAERNQLMALAVREGGKTFPNALGEVRETVDYLRYYAGRARALFEN
                     PIRLPGPTGEENELTMHGRGVFACISPWNFPLAIFTGQVAGALAAGNAVLAKPAEPTP
                     LIAAAAVRLLHRAGVPEDLLHLLPGKGSRLGRVLFADARLAGVALTGSTEAAWAINRA
                     LAARDGPLAQLIAETGGMNAMIVDSTALPEQVTRDVIASAFDSAGQRCSALRVLFVQA
                     DVADRMLELILGAMDELKLGDPFLLDTDIGPVIDEAAREPLEAHALRMTREAKLLRKL
                     KLGPEHEPGVFFPPHVFEIESIAALKREVFGPILHVVRYAGERLGDVCAAINATGYGL
                     TLGIHSRIEETASFVRAQVHAGNTYVNRNQIGAVVGVQPFGGEGLSGTGPKAGGPHYL
                     LRFALERTFTVNTTAAGGNAALLSSDRDGI"
BASE COUNT          367 a          643 c          666 g          372 t
ORIGIN      
        1 aggaaggcat ttttccgcaa tttgcaaccc acaacgcgca cacgctttcg gcggtgcagg
       61 tattggcggg gaacaatccg gatttcgaat ttcagcggct gcacggaatg ggcgaggccc
      121 tttacgaatt gtatcgcgag atgaagcagc cgggccgcgc cggtcctgcc acgcggatat
      181 acgcgccggt gggcacgcac gaagatctgc ttgcctatct cgtgcggcgg cttctcgaaa
      241 acggcgcgaa cacctcgttc gtcaaccgtc tcgccgatga cgaatcgccg gtcgccgaga
      301 ttatcgccga tcccgtcgaa cagcttgccg cttcaagccc gcggcgcagt tcgcgcattc
      361 caaagccgat cgacctgttg gccgatcgca ggaactcgac gggctttctg tggagcgatc
      421 cggaaatcgg cgcgccgatc atcgcccaga tgagcaaggc gctggagccg ccgcagcgtg
      481 ccgcgccgct catcggcggg aaggtgcgaa ccggcgttcc gcgggaagtt cgcaatccgg
      541 ccgatcggtg ccgtgtggtc ggcgaggtca tcgaggccgc cgaagccgac gcgatcgacg
      601 cgctcgctct cggacaccgt gcgcaggaag catgggacaa gcgcggcggc gatgcgcgcg
      661 cccgcatact cgagcaggcg gctgagcttt tcgaagccga acggaatcaa ttgatggcgc
      721 tcgcggtgcg cgagggcggc aagacgttcc ccaacgcatt gggcgaggtc cgcgaaacgg
      781 tcgactacct gcgttattac gcggggcgtg cgcgcgcgct gttcgaaaat ccgatcaggc
      841 tgcccggacc cacgggcgag gagaatgagc ttacgatgca cggccgcggc gtgtttgcct
      901 gcatctcgcc gtggaatttt ccgctcgcca tctttaccgg acaggtggcc ggcgcgctcg
      961 ccgccggcaa tgcggttctc gccaagccgg ccgagccgac tcccctgatt gccgcggccg
     1021 ccgtcaggct ccttcatcgc gccggcgttc cggaggactt gcttcacttg cttcccggca
     1081 aaggctcccg cctcggcagg gttctgttcg cagatgcgcg gctcgcggga gttgctctca
     1141 cgggctcgac cgaagcggcc tgggcaatca atcgcgccct cgcggcccgc gatggtccgc
     1201 tggcgcagct gattgccgaa accggcggca tgaacgcgat gatcgtcgat tccaccgcgc
     1261 tgcccgaaca ggtcacgcgc gacgtgatcg cgtcggcctt cgacagtgcc gggcagcgct
     1321 gcagcgcctt gcgcgtgttg tttgtgcagg cggatgtcgc cgaccgcatg ctggagctga
     1381 tcctgggcgc gatggacgag ctgaagctgg gcgatccgtt tcttctggac accgatatcg
     1441 gaccggtgat cgacgaggcg gcacgcgaac cgctggaggc gcacgccctg cgcatgaccc
     1501 gcgaggcaaa gctgctgcgc aagctgaagc ttgggcccga acacgagccg ggcgtcttct
     1561 ttcccccgca cgtgttcgaa atcgaatcca tcgccgcatt gaagcgcgaa gtgttcggcc
     1621 caattctgca cgtggtgcgc tatgccggcg aacggctggg agatgtttgt gcggcgatca
     1681 acgcgaccgg ttacggattg accctcggca ttcatagccg gatcgaggaa accgcatcct
     1741 tcgtccgcgc gcaggttcat gccggaaata cctacgtcaa tcgcaaccag atcggcgctg
     1801 tggtgggcgt gcagcctttc ggcggggagg ggctttccgg caccggaccg aaggccggag
     1861 gtccccatta cctcttgcga tttgcgttgg agcggacgtt tacggtcaac accaccgcag
     1921 ccggcggcaa tgcagcgctg ctgagttcag acagggatgg tatttgagaa atcactcgcg
     1981 caagaacctt ttgcctttgt cagactatta tatcaaaagg caccacgaca gaatattcca
     2041 gaattggc
//
LOCUS       DASZMC010000012         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2757841,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000012 DASZMC010000000
VERSION     DASZMC010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757841"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..790
                     /locus_tag="VG274_00190"
     CDS             143..790
                     /locus_tag="VG274_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWE05094.1"
                     /translation="MPDSQPSVPTGRNAVLALLKREAPVSADSLAQKLNLTGMAVRLH
                     LGALLAEGLVRSAVEPRPRGRPVQMWSTTPKADAQFADSHSGLAIDLIAQMKKAFGDE
                     GMDKILKQRTADQEKIYRTKTDSARSLKGKLDQLAKIRSAEGYMAEVRRDAATGDWLF
                     VENHCPICAAARLCAGLCREERALFNRVLGKDIKVERLSHILTGANRCAYRVTAT"
     gene            complement(848..1063)
                     /gene="rpsU"
                     /locus_tag="VG274_00195"
     CDS             complement(848..1063)
                     /gene="rpsU"
                     /locus_tag="VG274_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWE05095.1"
                     /translation="MQIIVRDNNIDQALKALKKKMQREGIFREMKLRGHYEKPSVKRA
                     RERAEAVRRYRKLQRKRAQREGVVPGK"
     gene            1203..1757
                     /gene="def"
                     /locus_tag="VG274_00200"
     CDS             1203..1757
                     /gene="def"
                     /locus_tag="VG274_00200"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWE05096.1"
                     /translation="MAILKIAQMGHPVLGQRAQDVADPAAPEIRRLVADMVETMIDAN
                     GAGLAAPQVHVPLRVVVFQAPGDRTEDDVDADEMYDHTAPLTVLINPVIEVVEPKLDG
                     GWEGCLSVPGLRGFVERPGHIHYRGFGTDGKLIARTAKGFHARVVQHECDHLDGVLYP
                     QRMRDLSKLIFESEIRHWVRRPEE"
     gene            1754..2407
                     /locus_tag="VG274_00205"
     CDS             1754..2407
                     /locus_tag="VG274_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HWE05097.1"
                     /translation="MTDTRSENDLKAAVLDAALKRVPDEGFIDSMLTAAGKDAGVGEE
                     MLLHLFPQGPLGLVEFFSEQGDAEMERSLAEQNLSAMKIRARISAAVLARIDAIRPHK
                     EAARRAGAFLTLPLHAATGMKLLYQTVDAMWRAAGDTSTDFNFYTKRGILAGVYSATL
                     LRWFNDSSEDESTTHEFLAARIENVMQFEKFKAGMRERTKDMPSFADVLKGFSAPKS"
     gene            complement(2388..3482)
                     /locus_tag="VG274_00210"
     CDS             complement(2388..3482)
                     /locus_tag="VG274_00210"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWE05098.1"
                     /translation="MSDAKRLSTPVPPGGTIGILGGGQLGRMLAMAAARLGLKTHIYS
                     PPNEDAPAFQVSDDHTSAAFDDKAALTKFAQACDVVTFEWENVPVATVEHIAQISSIL
                     PNAKTLGVTQDRFAEKSFIKSLGLATAPFVKVDSAEDAHAKVSGSAILKTRRMGYDGK
                     GQAKVKSREEAARALETFKGAPCILEGFVEFDYEASVIAARDADGSFAAYDPPENIHE
                     HHILRRSIVPSRLSAAQVDEAKSIARKIADALDYVGVFAVELFVMKDGSLLVNEIAPR
                     VHNSGHWTIDACLVSQFENHIRAVAGWPLGSTERHSDAVMENLIGEEAADWQALAKKG
                     GALHLYGKKEIRAGRKMGHVTYITPKTSAR"
     gene            complement(3479..3967)
                     /gene="purE"
                     /locus_tag="VG274_00215"
     CDS             complement(3479..3967)
                     /gene="purE"
                     /locus_tag="VG274_00215"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWE05099.1"
                     /translation="MTAPQIGLIMGSQSDWPTLRAASEILDKLGVAHESKIISAHRTP
                     DRMADYAKGAADRGLKVIIAAAGGAAHLPGMTASMTTLPVLGVPVESKALKGMDSLLS
                     IVQMPGGVPVATFAIGEAGAKNAALHAAAILALSDAALAKRLSEWRKALTDSVAETPI
                     NA"
BASE COUNT          786 a         1273 c         1306 g          851 t
ORIGIN      
        1 acaaccttgc ggcccgccgc tttcaggtcg ctcgcactta caccggtggc ctcgaacatc
       61 gtgcgctctt ttataaagct gtgactttca aaattatgga ggcatgcctt gctttaagca
      121 agtctaacct ttacaaagtg ccatgccgga ttcgcaaccc tcagtcccca ccgggcgcaa
      181 tgccgtgctg gcgctgctga aacgcgaggc gccggtttcc gccgattctc tggcgcaaaa
      241 gctgaacctg accgggatgg cggtgcggct gcatctcggc gcgctgctgg cggaagggtt
      301 ggtgcggtct gcggtcgagc cgcgtccacg cggacgtccg gtgcagatgt ggagcacgac
      361 gccgaaggcc gacgcgcaat tcgccgattc ccattccggg ctggccatcg atctgattgc
      421 gcagatgaag aaggctttcg gcgacgaggg catggacaag atactgaagc agcgcaccgc
      481 cgatcaggaa aaaatctatc gcacgaagac ggactcggcg cgctcgctta aagggaagct
      541 cgatcagctc gcgaagatcc gctccgcgga aggctacatg gccgaggtac gccgcgacgc
      601 cgccacaggc gactggctgt tcgttgagaa ccactgtccg atctgcgccg ccgcgcgtct
      661 gtgcgctggt ctctgccgcg aagaacgcgc gctcttcaat cgcgtgctcg gcaaagacat
      721 caaggtcgaa cgcctgtcgc atattctcac tggcgcaaat cgctgcgcct atcgcgtgac
      781 ggcgacctga aaaaaaaggc cgccccggga gggacggcct tttcaaaaac tttacggcct
      841 cgatcgatta cttgccgggc acaacgcctt cgcgctgcgc acgcttgcgc tgcagcttgc
      901 gataacggcg gacggcttcc gcacgctcgc gggcgcgctt gacgctgggc ttctcgtaat
      961 ggccacgcag tttcatctcg cggaagatgc cttcacgctg catcttcttt ttcagcgcct
     1021 tgagcgcctg gtcgatattg ttgtcgcgaa cgatgatctg caaagtcggg tctcccggcc
     1081 attttcgagg gtcgggctag atatcagacg gggtttggcc tgtccacctg tcccgcccgt
     1141 cattttggtg caaatcccac cgaatctttg accaaaagct cctccgaccc catattcaac
     1201 ccatggctat cctcaaaatc gcccagatgg ggcacccggt gctgggtcag cgcgcccagg
     1261 acgtcgccga tcccgccgcg cccgaaatcc gccgcttggt ggccgatatg gtcgagacca
     1321 tgatcgacgc caacggcgcc ggcctcgcgg cgccgcaggt tcatgtcccc ctgcgagtcg
     1381 tcgtgttcca ggcgccgggc gaccgcaccg aagatgacgt cgatgccgac gaaatgtacg
     1441 accacaccgc accgctgacc gtgctgatca atccggtgat agaggttgtc gaacccaagc
     1501 tcgatggcgg atgggaagga tgcctctcgg ttcccggcct gcgcggattc gtggagcggc
     1561 ccggccatat ccactatcgc ggcttcggaa cggacgggaa actgatcgcg cgcacggcca
     1621 aggggtttca cgcccgtgtc gtacaacatg aatgcgatca tcttgatggc gtgctctatc
     1681 cgcagcgcat gcgcgatctg tccaaactga tcttcgaatc cgaaatacgc cattgggtac
     1741 gaaggcccga agaatgacgg acacgcgctc cgagaacgac ctgaaagcgg cggtgctcga
     1801 cgcggcgctg aagcgcgtgc cggatgaagg cttcatcgac agcatgctca cggctgccgg
     1861 caaggatgcg ggcgtgggag aggagatgct gttgcatctg ttcccgcagg gaccgcttgg
     1921 tcttgtcgaa ttcttttcgg agcagggcga cgcggaaatg gagcgcagcc ttgcggaaca
     1981 aaacctttcc gcgatgaaaa tccgcgcgcg tatctcggcc gcggtgcttg cgcgcatcga
     2041 tgcgatccgg ccacacaagg aagccgcgcg ccgcgcgggt gcattcctga cactgccgct
     2101 gcatgccgcg acgggcatga agcttctgta ccagaccgtc gatgcgatgt ggcgcgctgc
     2161 cggcgatacg tcgaccgatt tcaacttcta taccaagcgc gggattctgg ccggggtcta
     2221 ttccgcgacg ttgctgcgct ggttcaacga ttcgagcgag gacgaaagta caacgcacga
     2281 gttcctcgcg gcccgcatcg agaacgtcat gcagttcgag aagttcaagg ctgggatgcg
     2341 cgagcgcacc aaggacatgc cgtcttttgc cgatgtgctg aaaggcttca gcgcgccgaa
     2401 gtcttaggtg tgatgtaagt gacgtgcccc atcttgcgac cggcgcggat ttcttttttg
     2461 ccgtagagat gcagcgcgcc gccttttttc gcgagcgcct gccagtcggc cgcttcctcg
     2521 ccgatcagat tttccattac ggcgtcggaa tgacgctcgg tcgaacccag cggccagccc
     2581 gcaacggcgc ggatatggtt ctcgaactgg ctgaccagac aggcatcgat cgtccaatgc
     2641 ccggaattgt ggacgcgtgg cgcaatctca ttcacgagca gagaaccgtc cttcattacg
     2701 aacaattcga ccgcgaacac accgacatag tcgagcgcat cggcgatctt tcttgcgatg
     2761 gatttggctt catccacctg cgctgcgctc aggcgcgacg gcacgatcga gcggcgcaga
     2821 atgtgatgtt cgtgaatgtt ctccggcgga tcataagccg caaacgaccc gtccgcatcg
     2881 cgcgctgcaa taaccgatgc ttcgtagtcg aattcgacaa agccttcgag gatgcacggc
     2941 gcgcccttga atgtttcgag cgcgcgcgca gcttcctcgc gggatttcac tttcgcctgg
     3001 cctttgccgt catagcccat acgccgcgtc ttcaagatcg cagatcccga aactttggcg
     3061 tgcgcgtctt cggcagaatc gaccttgacg aacggcgctg ttgcgagtcc gagcgatttg
     3121 atgaagctct tttcggcgaa acgatcttgc gtgacgccca gcgtcttcgc attcggtaaa
     3181 atggagctta tttgcgcgat gtgttctacg gtggcgaccg gaacgttctc ccattcaaac
     3241 gttacaacat cgcaggcttg cgcgaatttc gttagcgccg ccttgtcgtc gaaggctgcg
     3301 cttgtgtggt catcgctgac ctgaaacgcc ggcgcgtctt cgttcggtgg cgagtagatg
     3361 tgcgtcttta ggcccaatcg cgctgccgcc atcgctagca ttcggccgag ttgtccgccg
     3421 ccgagaatcc cgatggttcc gcccggtggg actggagtgg agagccgctt tgcgtcgctc
     3481 atgcgttgat cggggtctct gcgacggagt cggtcagggc cttgcgccac tcgcttagtc
     3541 gcttggcgag tgccgcatcc gacaaagcca ggattgccgc tgcgtgcagc gcggcgttct
     3601 tcgcgcctgc ttcgccaatc gcaaacgttg cgaccggaac gccgccgggc atctgcacga
     3661 tggagaggag gctatccatg cccttgagcg ccttgctttc aacaggcacg ccgagcaccg
     3721 gaagtgtcgt catcgatgct gtcatgcccg gcagatgcgc cgcgccgccg gcggcggcga
     3781 tgatcacctt caacccacgg tccgccgcgc ccttggcata gtcggccatg cggtccggcg
     3841 tgcggtgtgc ggagatgatc ttgctctcat gggccacgcc gagcttgtcg aggatttcgg
     3901 atgccgcgcg cagggtcggc cagtcggact ggctgcccat gatgagtccg atttgtgggg
     3961 cggtcatgct tcctgctttg gcaagggaaa ggaagcggcc cgttataagg gccgggcgag
     4021 atggagaaaa gtcctcttcg gaaggaattt aaccatttgg ggaccttcgt aaacgaattg
     4081 ttaaagccct cgatctagcg tttgcgggct ggagaagccg ggcggagcgg gggaatgaag
     4141 gtcgatagag taaacacggc gcgcggggtg cgtgcggtgg gcgccgcggc ctacgcgcgg
     4201 caggtcgagg ccgtgg
//
LOCUS       DASZMC010000013         5418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2483131,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000013 DASZMC010000000
VERSION     DASZMC010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483131"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VG274_00220"
     CDS             complement(<1..564)
                     /locus_tag="VG274_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE05100.1"
                     /translation="MKSVVVTGASTGIGWGCAKVLVEKGFHVLGSVRKEADAERLRKE
                     FGGNFTPLFFDVTDEAAVKQAASATASDLGNEPLFGLVNNAGIAVAGPLLYLEVDELR
                     RQLEVNVTGQLIVTQAFAPLLAEHVSEQNRAGRIVMISSVGGKNASPFVGPYNASKFA
                     LEGFSESLRRELMPLGIDVIVVAPGAVA"
     gene            621..1412
                     /gene="hisN"
                     /locus_tag="VG274_00225"
     CDS             621..1412
                     /gene="hisN"
                     /locus_tag="VG274_00225"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HWE05101.1"
                     /translation="MPDEETIAFAHRLADAAGAVIRPYFRQRLDIIDKGRTAFDPVTA
                     ADREAETAMRALIRSERPHDGILGEEHGLEIGSGRHRWVLDPIDGTRAFITGRPTWGT
                     LIALEDDGRRVLGIIDQPVLRERFIAYCDRGEFHSPAGAIALRTRECATLALAAVSTT
                     HPWGYFSSVERAGFEELCARAPMSYFGGDCYAYGLLALGHIDLIVEAALAAWDVAALI
                     PIVEQAGGAITDWSGAPVGEGGRIVAAGDARVHAQALEVLSGYLF"
     gene            complement(1463..2377)
                     /locus_tag="VG274_00230"
     CDS             complement(1463..2377)
                     /locus_tag="VG274_00230"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE05102.1"
                     /translation="MDELRTRFIASRDGKRLRTASWESAPGVARRGVCVILDGQTEFL
                     EKYGEVVQELAARGFAGAALDWRGQGGSDRTLSNPLKAHVGDFCEYDWDLQSFMNDVV
                     APMSDAPPLALGHSMGAHILLRALNANPRSFASAAMTAPMIRTDTSGFPAWLVRLICS
                     TYNLAGLTDEFVWGMRDRDPLHTKFEDNRVTSDRVRFARAQTLLAGQTQIRLAGPTWG
                     WFEAALRSMTAMQAPGFAEAIATPCLVFGAGRDRIVDTAAVRAFARRLPNGAYVELAD
                     AEHEIMMENDSIRARFWQAFDEFVGRYA"
     gene            complement(2384..2707)
                     /locus_tag="VG274_00235"
     CDS             complement(2384..2707)
                     /locus_tag="VG274_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWE05103.1"
                     /translation="MSSDQINQIITQMGTAIGADSGLGGTLKFDFGEPGSVLVDGKSV
                     PNKVTDGAGQNADCTISVSLETFEKMVKGELDGTSAFMQGKLRVAGDMGLAMKLGPIL
                     QKARG"
     gene            3035..3436
                     /locus_tag="VG274_00240"
     CDS             3035..3436
                     /locus_tag="VG274_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05104.1"
                     /translation="MSAAIPPPAGAVASKERDALGRCCFLLHFAVMIFVVGGWTMLWP
                     SALIFYLAFVPAVALQWQFNRNSCVLNNLESLMRTGTWRDAANEEEGAWLLTLARDVV
                     GLRVRPAQMDAFIYLVLLVLWGLGLAHLLSL"
     gene            3587..4051
                     /locus_tag="VG274_00245"
     CDS             3587..4051
                     /locus_tag="VG274_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HWE05105.1"
                     /translation="MRSFDFSPLFRSTVGFDRLFDLLDSVPQYDTAQTYPPYNIERTD
                     DTHYRITLAVAGFGEKDLNVEVREGVLTVQGKREESGEQGKTGFLYQGIAGRAFDRRF
                     QLAENVEVRGAKLENGLLHVDLERIVPDEQKPRRIPINGAEVKIIEGKARAA"
     gene            4129..5085
                     /locus_tag="VG274_00250"
     CDS             4129..5085
                     /locus_tag="VG274_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE05106.1"
                     /translation="MPSWHSALFARLPHLGRPGGPGRDIAELRRRYDTLFERFGAAPT
                     QVSFEAGQVGPIKGEWVRSPGSASHRLIVYLHGGGLVAGSPVTHRPLIASLCLAARAA
                     AFAPAYRLAPEYPFPAGLRDAVDTYRYLVQHGVAAGGIVVAGDEAGGGLAFSLLHAIR
                     NAGLPMPAACAAMSPWADLSLSGWSMLQNAKSDAALSWEMLFVSARHYLQKSTPADPY
                     ASPLFAPFREFPPLMVHAGSFEILRDDASRIGECAAETGTAVSVEIYDRMEHLFQAVP
                     GLPEAHISLERLGHFIQTRTRDAGTAAPLQPSQSPQGAKAGR"
BASE COUNT          931 a         1724 c         1743 g         1020 t
ORIGIN      
        1 cgccaccgcg cccggcgcga cgacgattac atcgatgccg agcggcatca gttcgcggcg
       61 caggctttcc gagaatcctt cgagggcgaa tttcgacgcg ttgtagggtc cgacgaaggg
      121 cgaagcgttc tttccgccca cggaggaaat catcacgatg cggccggccc ggttctgctc
      181 cgaaacatgt tcggcaagca ggggcgcgaa ggcctgggtg acgatgagtt gcccggtaac
      241 gttcacctcg agctgacggc gcaattcgtc cacctcgaga tagaggagcg gaccggccac
      301 cgcgatcccc gcattgttga cgagaccgaa cagaggttca tttccgagat ccgatgcagt
      361 agcggacgcg gcttgcttca ccgccgcttc gtcggtgaca tcgaaaaaaa gcggggtgaa
      421 atttccgccg aactccttcc ggagccgctc ggcgtcggct tctttgcgca cgctgcccag
      481 aacgtgaaag cctttctcga ccaggacctt ggcgcagccc cagccaattc cggtcgaagc
      541 tccggtcaca acgacgctct tcatcgctcc cctccgtgtt cgcgccgtgc aatatagcgg
      601 caaagcagga aagcgaagcc atgccggatg aggaaacgat cgcctttgcg caccggctcg
      661 ccgacgcggc tggcgcagtg atccggccct atttccgcca aaggctggac atcatcgaca
      721 agggcaggac ggcgttcgat cctgtgaccg ccgcagatcg ggaagccgaa acggccatgc
      781 gcgcgctcat ccgcagcgag cggccgcacg acggcatact tggcgaggag catggcctgg
      841 aaatcggcag cgggaggcat cggtgggtgc tcgatcccat cgacggcacg cgcgccttca
      901 tcaccggacg ccccacctgg ggcacgctga ttgcgctgga ggacgacggc cgacgcgtgc
      961 tcggcatcat cgatcagccg gtcctgcgcg agcgcttcat tgcgtactgc gaccgcgggg
     1021 aatttcattc gccggcaggt gcgatcgcgc tgcgcacgcg cgaatgcgcg acgcttgctc
     1081 ttgccgcggt gtcgaccacg catccctggg gttatttctc gagcgtggag cgcgccggct
     1141 tcgaagaact ctgcgcgcgt gcgcccatga gctattttgg cggcgattgc tacgcctacg
     1201 gcttgctcgc gctggggcat atcgatttga ttgtcgaggc ggcgctcgcg gcgtgggacg
     1261 tggcggcgct cattcccatt gtcgagcaag cagggggcgc aatcaccgat tggagcggcg
     1321 cgcccgtcgg cgaaggcggc cgcatcgtcg ccgccggcga cgcgcgcgtg catgcgcagg
     1381 cgctggaggt tctgtcgggc tatttgtttt gagtcgtttc gactgaattt cattcagaaa
     1441 aaatcattat gggataagcc gtctaagcat acctgccaac gaactcgtca aacgcctgcc
     1501 aaaaccgcgc acggatggaa tcgttctcca tcatgatttc gtgttcggcg tcggcgagct
     1561 cgacataggc gccatttggc aggcgacgcg cgaatgcgcg gacggccgcg gtgtcgacga
     1621 tgcggtcgcg gcctgcgccg aacacgaggc acggcgtcgc gatggcttcc gcaaatcccg
     1681 gcgcttgcat ggctgtcatc gaacgcaaag ccgcttcgaa ccagccccag gttgggccag
     1741 cgaggcggat ttgcgtctgt ccggccagaa gcgtctgcgc gcgcgcgaag cgcacacggt
     1801 ccgaggtgac gcgattgtct tcgaattttg tatgcagggg gtcgcggtcg cgcatgcccc
     1861 atacgaattc atccgtcaga ccggcgagat tgtaggtcga gcagatgagg cgcacgagcc
     1921 atgcaggaaa cccgcttgtg tcggtcctga tcatcggcgc cgtcatcgcc gcggaggcga
     1981 agctgcgcgg attcgcattg agcgcgcgca ggagaatatg cgcacccatc gaatggccga
     2041 gcgcgagcgg cggcgcgtcg ctcatcggag cgaccacgtc attcatgaag gactgaagat
     2101 cccagtcgta ttcgcaaaaa tcgccgacat gggctttcag cggattgctg agggtgcggt
     2161 cggagccgcc ctgtccgcgc cagtcgagcg ctgcgccggc gaacccccgt gcggcaagct
     2221 cctggacaac ctcgccgtat ttctcgagaa actccgtttg tccgtcgagg ataacgcaga
     2281 ccccgcgccg cgcgacgcca ggtgcagatt cccaactcgc cgttcgaaga cgttttccgt
     2341 cgcggctcgc gatgaaacgc gtccgcagct cgtccaagat gaactagccg cgggccttct
     2401 gcaggatggg cccgagcttc atggccaggc ccatgtcgcc cgccactctc agcttgccct
     2461 gcatgaaggc ggatgtgccg tcgagctcgc ccttgaccat cttttcgaag gtttcgaggc
     2521 tgaccgagat cgtgcagtcg gcgttctggc cggcgccgtc cgtcaccttg ttcggcacgg
     2581 atttgccgtc gacgaggacg ctgccgggct cgccgaaatc gaatttgagc gtgccgccga
     2641 gccccgaatc ggcgccgatg gccgtaccca tctgggtgat gatctgattg atctggtcag
     2701 agctcatgca ttggtccttc cgtccggcaa atgtcccgct ccgcgatttg agcatgcgcg
     2761 ccgtgtctgc acaaccggat gtcggcgcgt tttcgtcgcg tcggacggta cgccagcgcc
     2821 tcaacccgcg tcatggccgg ggcagcccgg cgccggcgat ttggcggctc actcgttgca
     2881 cgggccgccg ccgatgagtt tgccgatcgc gacgcgccgg aggacggctt ggactgggcc
     2941 cgtgctcaac atagtctcgg cgctggatga ccggggtgtc cgtggccaaa gacttcagca
     3001 acagttgttg cgcctccgcg caagcttctt cgccatgagc gcggcgatcc cgccgcctgc
     3061 cggcgcggtg gcgagcaagg agcgcgatgc gctcgggcgc tgctgcttcc ttctgcattt
     3121 cgcggtgatg atcttcgtcg tcggcggctg gacaatgttg tggccgagcg cgcttatttt
     3181 ctacctagcg tttgtgcccg cggtcgcgct tcagtggcag ttcaatcgca attcctgcgt
     3241 gctcaacaat ctggagtcgc tcatgcgcac cggcacctgg cgcgacgccg ccaatgagga
     3301 ggagggcgca tggcttctca ccctggccag ggacgtcgtc ggattacggg tgcggccggc
     3361 gcagatggat gcctttatct atctggtgct gctggtgctc tggggcctcg ggctcgcaca
     3421 tctgctgagt ctgtaagccg ggcgcggtcc ggttcttgaa gcgcggagtg gtccttccta
     3481 cctatcgaat gtccggaatg gcctgagggc ttccgggcag agtgagaggt tcggcccatg
     3541 atgggcgagc ccaaaccaca acgcttcgaa aaggaggctg tgaaccatgc gaagtttcga
     3601 tttctcccca ctgttccgtt cgaccgtcgg ctttgaccgg ctgttcgacc ttctggattc
     3661 cgtgccgcaa tacgacacgg cccagaccta tcccccctac aacatcgagc gcaccgacga
     3721 cacccattac cgcattaccc tcgcggtagc gggcttcggc gagaaggatc tgaatgtgga
     3781 agtgcgcgag ggcgtgctca ccgttcaggg caagcgcgaa gagagcggcg aacagggcaa
     3841 gacaggattt ctatatcaag gcattgccgg ccgcgcgttc gaccgccgct tccagctggc
     3901 cgagaatgtc gaagtccgtg gggccaagct cgagaacggc ctgctccatg tcgatctgga
     3961 gcgcattgtt cccgacgagc agaagccccg gcgcattccg atcaacggcg ccgaagtgaa
     4021 gatcatcgaa ggcaaggcgc gcgccgccta aatctgttcg gcgcagaaga tgacagatgt
     4081 cgggcatcgg aacatactga tgcccgacat ctgaacgccg atatccccat gccgagctgg
     4141 cacagcgcgc tcttcgcgcg tctcccgcat ttgggccgcc ccggcggtcc tggccgtgac
     4201 atcgctgaac tgcgacgccg gtacgatacc ctgttcgagc gctttggggc cgcaccgacg
     4261 caggtgagct tcgaagccgg acaggtgggt cccatcaagg gcgaatgggt ccggtcgccc
     4321 ggttcggctt cccatcgtct cattgtgtac ttgcacggcg gcggcctggt cgcgggttcg
     4381 ccggtgacgc accgtccgct gattgcaagt ctgtgtctcg cggcgcgcgc ggcggcgttc
     4441 gccccggcct atcgtctcgc tccggaatat ccttttcccg ccggccttcg cgacgccgtc
     4501 gacacctatc gctatcttgt gcagcacgga gttgccgcgg gcggcatcgt cgtcgcggga
     4561 gacgaggcgg gaggcggcct cgctttctcg ctcctgcacg cgatccgcaa tgccgggttg
     4621 ccgatgcccg cggcttgcgc ggcgatgtcg ccctgggccg atctttccct gtcgggatgg
     4681 tcgatgctgc agaacgcgaa gagcgacgcg gcactttcct gggagatgct gttcgtcagc
     4741 gcgcggcatt acctgcagaa gagcacgcct gccgatccct atgcctcgcc cctgttcgcg
     4801 ccgttccgcg aatttccccc gctcatggtc catgccggaa gcttcgagat cctgcgcgac
     4861 gatgcgagcc gcatcggcga atgcgccgcc gagacaggca cagcggtcag cgtggagatt
     4921 tacgaccgga tggagcatct ctttcaggcg gttcccggcc tgcccgaagc gcacatctct
     4981 ttggagcgtc tcggtcactt catccagacg cgcacgcggg acgcgggcac ggcggcgcca
     5041 cttcagccct cccaatcgcc gcaaggcgca aaagccggaa ggtgaagcgg cgtcacggtt
     5101 ggcattttgc ggggccgccc cgttcatcgg atgaaacggg caaatcttga gctggcatgg
     5161 cctgttacgg gtctagcgcg ccgctcggaa atgcggttaa ggtgcctcgg tacgcggtga
     5221 cacgctaggg gcggcgccgc ggctgatgtg gggcccccgc cgtatcggcc cttgagaaat
     5281 accggccttt aacgacgaga ggtacgcatt gaacaagcgc gcgctgatgc tttccgccgc
     5341 cacggcctcg ttgctcgccg ggcaagtcct tgctgataca gacatcacca agcaggtgac
     5401 cacgccgcaa accaccag
//
LOCUS       DASZMC010000014         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3853765,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000014 DASZMC010000000
VERSION     DASZMC010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3853765"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VG274_00255"
     CDS             complement(<1..767)
                     /locus_tag="VG274_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWE05107.1"
                     /translation="MKDFRACAVIPTHNHVAALATILLRLEEVGLPAIVIDDGSDPEI
                     GVQIAAACVGRAGAEYRCHAFNGGKGFAVMCGVARARELGFSHAVQVDADGQHDLQSL
                     DALLEMARLNPTAIVSGIPRFDQPIPLARRIGRPITSFWVAVNSLSLRMPDAMCGFRV
                     YPIDATFNLVRRSVRGRRMDFDVEILVKAHWAAIPLAPVPVGVRYPKDNLSNFDVWRD
                     NLLLSLLHTRLFFGMLLRLPRLTFRRRTKIRLPDTKPV"
     gene            complement(764..>1796)
                     /locus_tag="VG274_00260"
     CDS             complement(764..>1796)
                     /locus_tag="VG274_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05108.1"
                     /translation="TLVTGPAHLTRLPPAIGGAAPGLVFSSGAALPFAAAQAARRQLR
                     SLPIEILGSTETGGVAWRRQENEDALWSPLPGVRIEAGDGDRLCVRSPFTDSDKAVST
                     GDLFDRLGEKFRLKGRIDRVAKIDGKRVSLARVEEALLAQPIVEAAAAVRLPERRGAL
                     GAVVELNPEGKAALVEKGAFRLSRDLRHALAERLEPAERPKHWRFGAIPFDRQGKRVE
                     DLLRAVFDRNADEKMGRGSIREIEAEQSTICVELTPDMVWFEGHFPDQPVLAGIAQVH
                     MAVLWAQQVWGWRPHGGNVSQLKFRRVLRPGDTVQLRLERRLNQQRLRFSYNLDEVVA
                     SEGIIGGSA"
BASE COUNT          353 a          590 c          542 g          311 t
ORIGIN      
        1 acgggctttg tatcgggcag cctgattttc gtccgccgcc ggaaagtcaa tcgagggagg
       61 cgcaaaagca tcccgaaaaa gagccgcgtg tgcagaagcg agagcagcaa attgtcgcgc
      121 cacacatcga aattcgagag attgtctttc ggataccgca cgccgaccgg aaccggggcg
      181 agcgggatgg ccgcccaatg cgctttcaca agaatctcga cgtcgaaatc catccggcgg
      241 ccgcggaccg agcggcgcac aagattgaac gttgcgtcga tcggatagac gcgaaagccg
      301 cacattgcgt ccggcatgcg cagcgagagc gaattgaccg cgacccagaa actggtgatg
      361 ggccgtccga tgcgccgcgc gagcgggatg ggctgatcga aacgcggaat cccgctgacg
      421 attgcggtgg gattgagacg cgccatttcc agaagcgcgt ccaggctctg cagatcatgc
      481 tggccgtcgg cgtcgacctg gacagcatgg ctgaagccca gctcgcgcgc ccgcgcgact
      541 ccgcacatca ccgcgaatcc cttgccgccg ttgaatgcat ggcaccgata ctcggcgcct
      601 gcacgcccga cgcacgcggc ggcgatctgc acgccaattt cgggatcgct gccatcgtcg
      661 atcacgatgg cgggaagtcc cacttcctcc agccgcaaca ggatcgttgc gagcgccgcg
      721 acgtgattgt gcgtcggtat gaccgcgcag gcccgaaaat ccttcacgcg gatcccccga
      781 ttataccttc ggaggcgacc acctcgtcga ggttgtagga aaagcgcaga cgctgctgat
      841 tcagccgccg ctcaagtctc agttggactg tatcgccggg ccgcaatacg cgccgaaatt
      901 tcagttgtga cacatttccg ccgtgtggtc tccaacccca cacctgttgc gcccaaagca
      961 ccgccatgtg gacctgtgca atgcccgcaa gcacgggctg atccggaaaa tggccctcga
     1021 accacaccat gtcgggcgtg agttcgacgc aaattgtaga ctgctccgct tcgatctccc
     1081 ggatgctccc gcgccccatt ttttcatccg cgtttcgatc gaagacggcg cgcagcagat
     1141 cctcaacccg cttgccctgg cggtcgaaag ggattgctcc aaaacgccaa tgcttggggc
     1201 gctccgccgg ttcaagcctc tcggccagcg catggcgcag atcccgtgac aggcggaagg
     1261 ctcctttctc caccagtgcg gctttgcctt ccgggttgag ttcaacgacg gcgccgagcg
     1321 cgcccctgcg ctcgggcagc ctgacggcag ccgccgcttc gacaatcggc tgcgccagca
     1381 atgcctcttc gacccgcgcc agcgaaacgc gtttgccgtc gatcttcgcg acacggtcga
     1441 tgcgtccttt cagtcggaat ttctccccca ggcggtcgaa caaatcgccc gtggaaaccg
     1501 ctttgtccga atcggtgaac ggcgaacgaa cgcaaagccg gtctccgtcg ccggcctcga
     1561 tcctgacccc gggaagcggg ctccacaacg catcttcgtt ttcctgccgg cgccaggcaa
     1621 cgccgccggt ttcggtgctg cccagaattt cgatcggcaa agaacgaagt tgccgcctcg
     1681 ctgcttgcgc agcggcgaac ggcaaagccg cgccggacga aaagacaagg ccgggtgcag
     1741 cgccgccaat tgccggggga aggcgggtca gatgtgccgg gccggttaca agcgtt
//
LOCUS       DASZMC010000015         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2759817,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000015 DASZMC010000000
VERSION     DASZMC010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759817"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VG274_00265"
     CDS             complement(<1..251)
                     /locus_tag="VG274_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWE05109.1"
                     /translation="MSMRFTEIGQQLRAYRLESGLRAEEIAARLGVSRAALYRYEKGE
                     VIKLDTIKRLAELLKISPLSLLGIGVEYYNRPIGYFERSR"
     gene            complement(462..1373)
                     /locus_tag="VG274_00270"
                     /pseudo
     CDS             complement(462..1373)
                     /locus_tag="VG274_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01505.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
     gene            complement(1573..2352)
                     /gene="otnI"
                     /locus_tag="VG274_00275"
     CDS             complement(1573..2352)
                     /gene="otnI"
                     /locus_tag="VG274_00275"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF043033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HWE05110.1"
                     /translation="MPRFAANLSMMFNEVPFLDRFAAARHAGFEGVEFLFPYEFSPAV
                     LRERLSGEGLSQVLFNLPPGDWANGDRGTASLPGRQAEFRESVKQALGYAAALDCRQM
                     HCMAGIVPADVSLTTAAAVYAANLAWAAEQAKPAGVRLVIEPINHRDMPGYFLNTQAQ
                     GAAIVEAIGTDRLGLQFDIYHVQITEGDITKRMERFMPLIAHMQIADVPARNEPGTGE
                     IGWRYVFRRMDELGYQGWVGCEYRPAAGTVPGLVWREQFNV"
BASE COUNT          474 a          858 c          790 g          519 t
ORIGIN      
        1 cgggatcgtt caaaatagcc gatgggccga ttgtagtact cgacgccaat tcccagcagc
       61 gaaagtggcg agattttaag cagttcggcg aggcgcttga tcgtgtcgag cttgatgacc
      121 tcgccctttt cgtaccgata gagcgccgcc cgggagaccc ccaggcgcgc ggcgatctcc
      181 tcggcacgca aacccgactc gagacggtag gcccgaagct gctggccgat ctcggtgaaa
      241 cgcatcgaca tgtaagccgc ccccacgcaa cttatacgcg caactagctg aaaaatcacc
      301 accagatgta agaccaaacc ctaccaccac ccggaaaaaa tatccccgtg gaggcgtgcc
      361 tcgtctcaga atcgcgtggc taatacagtt tatggggagc gcactctaaa cctcaacccc
      421 ttttcatgtg atgcgactcg tttcacgacg caacccgatg attatttcgc ttctcggatg
      481 ctatgggccg ccatgccttc gattgccagt acgagtgccg aatggtctag gtcggcgccg
      541 ccgtgggcga tgcaggatgc gagcatctgc tgcgtgaccg cggtgttggg caacgccagg
      601 ctaagcgcct tggcggacga cagcgccaag cctaggtcct tctggtgcag cttgatcttg
      661 aacccggggt tgaacgtgcc ctggagcatc cgttcggcgt gtacctccag aacccgcgac
      721 gacgcgaatc cgcccatcaa cgcctggcgc accttggccg gatcggcccc tgccttcgag
      781 gcgaatacca gcgcctccga cactgcctgg atgttcagcg cgactatgat ctgattggcg
      841 accttgcaaa tctgcccggc gcccggctcc gggccgacat gggtgatatt cttgcccatg
      901 agttcaaaca gcggcttggc acgcgcgaag gcggcctcgc ggccgccgac catgatcgtc
      961 agcgtcgcct gcttcgcgcc gacttcgccg ccggataccg gcgcatcaag gtagtcgcag
     1021 cctttggcgt tgatccgcct ggcgtaatcc ttggtttcga tcgggctgat cgaagacata
     1081 tccatcacca gcgtgccctt gcgcagccct tcggcggcgc cgttgtcacc gaacagcacg
     1141 gcctcgacat caggcgtgtc gggaagcatg aggatcagca cgtctgacgc cgcggcgacg
     1201 tcccttgcgg tcgcaacgac tctggcgatg ctgcgctctt cgtcccgcag gctcgcccgc
     1261 tccgggacga tcaggtcatg cccggcgttc ttcagattct gcgccattgg ccggcccatg
     1321 atgccaagac cgataaatcc gattttcatg ttcgttgctc ctttcgcgtt ggcttggcga
     1381 tgctcgggcg acggcttcct gtgcgtccct ctagtgtccc gattcggaag ttcgctgtgc
     1441 ctggatcggt cgccttacga acttccgaat cgaccaggac actagcaaag cattgttctc
     1501 tagtgtggtt tatggttccg agggccgctc cggtgctcgc tccacagtgt ggcggacttc
     1561 ggaaccacca cactagacgt tgaattgctc gcgccagacg aggcccggga cggtccctgc
     1621 cgccggccgg tattcgcaac cgacccagcc ctggtagccc agttcatcca tccgccggaa
     1681 cacgtagcgc cagccgattt ccccggttcc cggctcgttg cgagccggaa catcggcaat
     1741 ctgcatgtgg gcgatcagcg gcatgaaccg ctccatgcgc ttggtgatgt caccctcggt
     1801 gatctgaacg tggtagatat cgaactgcag gcccagccgg tcggtgccga ttgcctcaac
     1861 gatcgccgca ccttgggcct gggtattcag gaaataacct ggcatgtcgc ggtggttgat
     1921 tggctcgatc accagccgca cgccggctgg cttggcttgt tccgccgccc aggcgaggtt
     1981 cgccgcatac accgctgccg ccgtcgtcag cgatacatcg gccggcacga tccccgccat
     2041 gcaatgcatc tgccggcaat ccagcgccgc ggcatagccc agcgcctgct tcaccgattc
     2101 gcggaactcc gcctggcgtc caggcaggct cgccgttcca cggtccccgt tcgcccagtc
     2161 gccgggcggc aagttgaaca gcacctggct cagcccctcc ccgcttaagc gttcgcgtag
     2221 caccgccgga gaaaattcgt agggaaacag gaactccaca ccctcgaacc cggcgtgacg
     2281 ggcggcggcg aaacggtcga ggaagggaac ctcgttgaac atcattgaca ggttggcggc
     2341 gaaacgcggc atggcggacc tcctcctggt attggcatct gcgatcggct gcgatcgcgg
     2401 gcaggatgcc ctcttgtctg cccgtccgaa acacgcaacg actgatagac gccgacgcgc
     2461 tttcgggtgg cgccgactcc ggcgcaagtt acaagcgcgc agcggccttg gctaggcgat
     2521 ggctacggcg gcgagcttgc gagcaatcgc ggatggagag gcatgatcgc tgaacggatc
     2581 ctgatgggcc tgctgctggg cggcttggcg atcggctgcg tgctggtgct gtatccgttc
     2641 t
//
LOCUS       DASZMC010000016         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4133234,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000016 DASZMC010000000
VERSION     DASZMC010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133234"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1482)
                     /locus_tag="VG274_00280"
     CDS             complement(94..1482)
                     /locus_tag="VG274_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05111.1"
                     /translation="MNTATGLGRRPHAQASLIAAGTAIVLGWAVSVALNWPGQLSYDS
                     VAQLHDGRTGHYNAWHPPIMAWMLGLADAALPGTGLFILFDAFLLFGGMLSILWIAPR
                     VSWGAVAIAAVAVALPQFVLYQGIVWKDVLFADAAIAGFVLLAHAASRWASARLRWAL
                     IVAAFLLFALATLARQNGAIALLFGTVALLAVARQHDVGWRGAIVRSIGTGTAAALVV
                     LAASLALAQRASGGSGPLGQIRLLQLYDLIGEAKAEPGLGLDVLSRTNPDLERLIRSD
                     GVRLYTPARNDTLYGSTELLNEFASTSPDAIASQWWSVMVHHPLDYLGVRAHVFAWTF
                     VTPDPIGCEDWYVGVTGFPQYLHELGLERRIRPQDRALGNYANLFVGTPIFSHATYAL
                     LAAALLFVLLRRRRPADLAIASLLVCALVFTASFFAISIACDYRYLLFLDLATFASCL
                     YCGVTWNRSLYN"
     gene            1724..>3169
                     /locus_tag="VG274_00285"
     CDS             1724..>3169
                     /locus_tag="VG274_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HWE05112.1"
                     /translation="MIDKPVFFDASGRRASRMSVFGRVVAVVTTFIGIVFIGSLIVEP
                     HVAGPNLPGRLSAINPVELVRRAFDPAMMNAAAKLAAEARAERAKLVRAWRAQRALVA
                     QAHSAKTALRPFPNRPLTVGFYLSTDELGYPDLKRVVSKLDWFVPAWMNLDGPDMKLV
                     RNVDKRAISAIAHAKPNVAILPLVQNASNAVWNGQGMAELLSDPVRRHILLNGVVTYL
                     AKNKFQGAVVDFESLPQSAYRDLATFLGEMKAAFKLHGWLVVVASPFDDDSWPYADYA
                     NLVDYTLLMAYDEHDDAADPGSIAGQSWFEENLDERMAVLSPGRTIIGLGNYGYDWSE
                     GKGEAMSFQDAVIAARDSQARIIFDDRTNNPHFSYLEDDNSKHEVWFLDGVTAYNQIH
                     AADPYQPAGYALWKLGSEDPSTWTVLGRRYGTPAPGRLRDIPTSQDVDFEGVGEILDV
                     EAHPTPGARALDIDRDTGDIDDETYTKLPTPY"
BASE COUNT          677 a          978 c          972 g          542 t
ORIGIN      
        1 catgagcttc atctaaccga caggttgaac tcgatcgcgg cgggagcggg ggaggaaggg
       61 cggtcacacg aacaaagaat caatgaaaat cagttaattg taaagcgatc gattccacgt
      121 tacgccgcaa taaaggcagc tggcgaacgt ggcaagatcc aaaaacagca ggtagcgata
      181 atcgcaggcg atcgagatcg caaaaaaact cgcagtgaag actagcgcgc agaccagcag
      241 cgacgcgatg gcgagatcgg ctggacgccg gcgcctcagc aacacgaaca acagggccgc
      301 agcgagcagt gcataggttg cgtgcgaaaa tatcggcgtc ccaacgaaaa ggttcgcata
      361 atttccgagc gcccgatcct gtgggcggat ccgacgttcg aggcccagtt cgtgcaggta
      421 ttgcggaaat ccggtcactc cgacgtacca gtcttcgcaa cctatgggat caggcgtgac
      481 gaaggtccag gcgaagacat gcgcgcgcac gccgagatag tcgagcggat ggtgcaccat
      541 gacgctccac cattgcgatg caattgcgtc cgggctcgtg ctcgcgaatt cgttcagaag
      601 ttccgttgag ccgtacaggg tgtcgttgcg cgcgggcgtg tagaggcgca cgccgtcgga
      661 gcggatcagc cgctcgagat cgggattcgt gcgtgaaagt acgtccaggc cgagcccggg
      721 ctctgccttc gcctctccaa tcagatcgta aagctgtaga agcctgatct gcccaagtgg
      781 gcccgagcca ccgctcgcgc gttgcgcaag cgcaaggctt gcggccagca cgaccagcgc
      841 cgcggctgtt cccgtcccaa tgctacggac gatggcgccc cgccagccga cgtcgtgctg
      901 tctcgcgacc gcaagaagcg caaccgtgcc gaatagcagc gctatcgcgc cgttctggcg
      961 cgcaagcgtt gccagggcga aaagcagaaa cgcggcaaca atcagcgccc atctcagcct
     1021 cgccgaggcc cagcgcgagg ccgcatgggc gagcagcacg aagcctgcga tagccgcgtc
     1081 ggcaaacagg acgtccttcc agacgatgcc ctgatagaga acgaattgtg ggagggcgac
     1141 ggcgactgcg gcaattgcga ccgctcccca ggacacgcgc ggcgcaatcc agaggatcga
     1201 cagcatgccg ccaaagagca ggaaagcgtc gaacagaatg aaaagtccgg tgccaggaag
     1261 cgccgcatcg gccagaccca gcatccaggc catgatcggc ggatgccagg cgttgtaatg
     1321 cccggtacgg ccatcatgca gttgcgcgac ggaatcgtag gaaagctggc cgggccagtt
     1381 cagggcgacg gaaaccgccc agccgaggac aatcgcggtc cctgccgcga tcaggctcgc
     1441 ctgggcgtgc gggcgccgtc caagtccggt tgcggtgttc accccgaccc tcctccgatt
     1501 gagcgacgat tgcagttttt gacttgaaaa aaaatcactc tcgccctgcg ccgaattgcc
     1561 tgtgcagcgc cgatttgcct tgaaaatgcc ggactggagg cattacctga tgtcggatgg
     1621 gggattacat tttttctgcg tgacctgacg cactgcggcc caaattcgat ggtccccgcg
     1681 catggcatcg gtcggtgggg cggagcccgg ttgcggacct gacatgatcg acaagccggt
     1741 cttcttcgac gcaagcgggc gccgggcatc gcgcatgtcc gttttcggac gcgtcgtcgc
     1801 ggtcgtcaca accttcatcg gcatcgtctt catcggcagc ctgatcgtcg aaccgcacgt
     1861 cgccggccca aatcttcccg ggcgtctcag cgccatcaat ccggtcgaac ttgtgcggcg
     1921 cgccttcgat ccggcgatga tgaacgctgc cgccaaactc gcagcggaag cgagggccga
     1981 acgcgcaaaa ctcgtgcgcg cgtggcgcgc acagcgcgcg ctggtcgcgc aagcccattc
     2041 tgcaaaaacg gcactgcggc ccttccccaa ccgtccgctc accgtcggat tctatttgag
     2101 caccgacgaa ctcgggtacc ccgacctgaa gcgcgtagtg tcgaaactcg actggttcgt
     2161 tccggcatgg atgaatctcg acgggcccga catgaagctt gtccgcaacg tggacaagcg
     2221 ggcaatttcg gcgatcgcgc atgccaaacc caatgtcgcc attctgccgc tggtgcagaa
     2281 cgcctcgaac gcagtgtgga acgggcaggg aatggcggaa ctgctctcgg atccggtgag
     2341 gcggcacatt ctcctgaacg gagttgtcac ctatctcgcc aaaaacaaat ttcagggtgc
     2401 ggttgtcgat ttcgaaagcc tgccgcagag cgcctatcgc gatctcgcga cttttctcgg
     2461 cgaaatgaag gcggcattca aactgcatgg ctggctcgtc gtcgtcgcct cgcctttcga
     2521 cgacgacagc tggccttacg ccgattacgc aaacctcgtc gattacaccc tgctcatggc
     2581 ctatgacgag catgacgacg cggcggatcc cggaagcatt gccggccaga gctggttcga
     2641 ggagaatctc gacgaacgca tggcagtgct ctcgcctggg cgcaccatca tcggtcttgg
     2701 aaattacggc tacgactggt cggaaggcaa aggcgaggcc atgtcgttcc aggatgcggt
     2761 gattgccgcg cgcgattcgc aggcgcgcat cattttcgac gaccgcacca acaacccgca
     2821 tttctcctat ctcgaagacg acaatagcaa acatgaggtg tggtttctcg acggcgtgac
     2881 cgcctacaac cagattcacg cggcggatcc gtatcagccc gcgggctatg cgctatggaa
     2941 gctcggcagc gaggatcctt ccacctggac cgtactcggc cgccgctacg gcacccctgc
     3001 gccgggcagg ttacgcgaca tcccgaccag ccaggacgtc gatttcgagg gtgtgggcga
     3061 aatcctcgat gtggaggcgc accccacccc gggcgcgcgc gcgctggata tcgatagaga
     3121 tacgggcgat atcgacgacg agacctacac caagctgccg acgccgtac
//
LOCUS       DASZMC010000017         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4953998,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000017 DASZMC010000000
VERSION     DASZMC010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4953998"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..912
                     /locus_tag="VG274_00290"
     CDS             379..912
                     /locus_tag="VG274_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWE05113.1"
                     /translation="MAKLVFGLSQSLDGYVDHLELPAPGPALFRHFTEWVRGLRGSVY
                     GRRTYEIMRYWDEDRPGWSAEESDFAAVWRRQPKWVVSRSLKSVGPNATLVEDDIEEV
                     MRGLKARLTGEIEVAGPDLARSLTGLGLIDEYRLYFRPFVLGHGTPFFAGPRPPLRLV
                     AGDPVGEDAMRLTYVPA"
     gene            1060..1491
                     /locus_tag="VG274_00295"
     CDS             1060..1491
                     /locus_tag="VG274_00295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWE05114.1"
                     /translation="MKSIAVAMVLVLGAAAPVALTATAAQAAQQDQFKSIDVKQGTGA
                     VAKAGQQVTVNYTGWLYEHGARGKKFDSSLDHGTPFTFALGAGNVIPGWDRGVAGMKV
                     GGKRTLIIPAELGYGAQGTPDGTIPPGATLIFDVELLKVQS"
     gene            complement(1563..1766)
                     /locus_tag="VG274_00300"
     CDS             complement(1563..1766)
                     /locus_tag="VG274_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05115.1"
                     /translation="MPDPQPKLAEIDARIADIRENLRELVEQAAAYSGAADDDLVSQR
                     IADQEALLERLTDERDKLAQAGR"
     gene            complement(1923..2363)
                     /locus_tag="VG274_00305"
     CDS             complement(1923..2363)
                     /locus_tag="VG274_00305"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HWE05116.1"
                     /translation="MKMEDNTRIVTAARVKLPATLALKWSDGTRAEIDLARTMRDRHF
                     RSLRNPGVFGKVRIGEWGHSVEWPSGVELGAETLWLETLAATGHDDARVFLEWRLRHG
                     LSLSKAAEALGISRRMVAYYSNGEKTVPRAILLACKGWEVGHAA"
     gene            complement(2360..2587)
                     /locus_tag="VG274_00310"
     CDS             complement(2360..2587)
                     /locus_tag="VG274_00310"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HWE05117.1"
                     /translation="MVTVYRGSGWKIAVYGREHGVPHFHIEGPDFRCSVRIDTLEVIV
                     GTAPPDVLKSACAWARQNRTHLNARWHELNA"
BASE COUNT          462 a          832 c          890 g          525 t
ORIGIN      
        1 aaaaggacgg ggacgcctaa tctattatag taacgtgggc gtgtttgctt cattgctacg
       61 atcagcaaaa tagatcaggc gtccccgtat ttcgtgttca acctcgcatg gacgttcatg
      121 aaaagatgtg ggctcacgtc agccttgtgg ggaggctgga aggctcgttg ggccggagtg
      181 ccactccgat gccccagatg ggtgcatgtc ccggcaggtt gttgaagaat tggctgacac
      241 cacaagacct ttcacaggtc acgacgccat gaacggcttt atcgtgacgg ccgacaatgg
      301 cggagccgca ttgcggttca cttctgcgca acggcggctc atgctattcc gaagaacttc
      361 gggtggagat tttgcagcat ggcgaagctc gtgttcggat tgtcgcagtc tctggacggc
      421 tacgtcgacc acctggaatt gccggcaccc ggtcccgcgc tatttcgtca cttcaccgag
      481 tgggtgcgcg gcctgcgcgg cagtgtgtac ggtcgccgca cctacgagat catgcgttat
      541 tgggacgaag accgtcctgg gtggagcgcg gaggaaagcg atttcgcggc ggtgtggcga
      601 cgtcaaccga agtgggtcgt gtcgcgctcg ctgaagtcgg tcggccccaa cgccacgctt
      661 gtcgaggatg acatcgagga ggtgatgcgt gggctgaagg cgaggctcac tggggagatt
      721 gaagtcgccg gaccggatct ggcgcgaagc ctgaccggcc ttggtcttat tgatgagtat
      781 cgactctact tccgcccttt cgtgcttggt cacggcacgc cattcttcgc cggcccccgg
      841 ccgccgctcc gccttgtggc cggcgatccg gttggcgagg atgcgatgag gttgacgtac
      901 gtccctgctt gatcgcgcga catgcccgac ggactgcgcc gccgcgtcag ggccgtcgaa
      961 cgaggcgcgc aagcgcgggg gtggcggggg aggggcaaag gctttatgca atcggccgga
     1021 aaactttctc cacaccgcaa acagggatct ctcatgcacg tgaaatcgat tgccgtggcg
     1081 atggtgctcg ttctcggcgc ggctgcgccg gtggccctta cggccacggc cgcccaggcc
     1141 gcccagcagg atcagttcaa gagcatcgac gtcaaacagg gcaccggcgc cgttgcgaag
     1201 gcgggacagc aggtgacggt caactatacg ggctggctct atgagcacgg cgccaggggc
     1261 aagaaattcg attcgtcgct cgatcacggg acgccgttca ccttcgcgct cggcgccggc
     1321 aatgtcattc ccggttggga caggggcgtg gccggcatga aggtgggcgg gaagcgcacg
     1381 ctgatcattc cggccgagct cggctatggc gcccagggca ctccggacgg cacgatcccg
     1441 cccggcgcga cgctgatctt cgacgtggaa cttctgaagg tgcagtcgta agcgcggcat
     1501 gaatttggat tgaatcgccg ccgagccgcc acacgttccg ctggcgtccg gagcccgatg
     1561 tctcatcgcc ctgcctgggc gagcttgtcg cgctcgtctg tcaggcgctc gagcagtgct
     1621 tcctgatcgg caatacgttg cgaaaccaga tcgtcgtcgg cggcgccgga ataggcagcc
     1681 gcctgctcga ccagctcgcg caggttttcc cggatgtccg cgatgcgcgc gtcgatttct
     1741 gcgagtttcg gctgggggtc cggcatggct cggtcatctc ctgtgcaggc atccggcgcc
     1801 gcgcgttcga agcgccgcgg ggttgcgtgt cgcggcaatc ttatcccggc tcttcctcgt
     1861 gccaagagcc cggcgcgtct gcggcgtcgc acgggacgcg gccgccgcgc cggcgcgggg
     1921 ggtcacgccg cgtggccgac ttcccagccc ttgcaggcca ggaggatcgc gcggggaacg
     1981 gttttttctc cattcgagta ataggccacc atgcggcggg aaatgccgag cgcttcggcc
     2041 gccttcgaca gcgagaggcc atggcgcaga cgccattcga gaaatacccg cgcatcgtca
     2101 tggccggtcg ccgccagcgt ttcgagccag agcgtttctg cccccagttc gacgcccgag
     2161 ggccattcca cgctgtggcc ccattcgccg atacggactt tcccgaagac ccctggattg
     2221 cgcagggaac ggaaatgcct gtcgcgcatc gtcctggcga ggtcgatttc ggcgcgtgtg
     2281 ccgtcggacc atttgagggc aagcgttgcc ggcaatttca ccctggcggc cgttacgatc
     2341 cgggtattgt cctccatctt catgcgttca gctcatgcca tcttgcgttg aggtgcgtcc
     2401 ggttctgccg cgcccatgca caggcgctct tgagcacgtc tggcggcgcc gtcccgacga
     2461 taacttccag cgtgtcgata cgcaccgaac agcgaaaatc cggaccctcg atatgaaaat
     2521 gcggaacgcc atgctcgcgc ccgtaaacag cgatcttcca tccggaaccg cgatatacgg
     2581 ttaccatgcg ggttggtagt gcaactgctg caccggtgca agcctctcca ctgcaggcac
     2641 gcttcgcgcg aggggggaaa gcaaatgtaa gcagctcctt tcctcccccg cgcgcagcgc
     2701 gggggagcg
//
LOCUS       DASZMC010000018         6814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3035135,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000018 DASZMC010000000
VERSION     DASZMC010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3035135"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..577)
                     /gene="thpR"
                     /locus_tag="VG274_00315"
     CDS             complement(59..577)
                     /gene="thpR"
                     /locus_tag="VG274_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWE05118.1"
                     /translation="MALALPDPVAESLLALQGGVPGARWMTREQLHVTLRFIGEVDGR
                     DAIAIDDALATIRAQRFSLELKGIGEFGGRNPRALWAGVRDGEQVAHLQRKIESAVQR
                     IGLPPEERKFVPHVTLARLRAAPRDRVITFLAAHALYVSGAFEIGAFILYSSILSPNG
                     SLYRPERAYALG"
     gene            complement(589..2133)
                     /gene="murJ"
                     /locus_tag="VG274_00320"
     CDS             complement(589..2133)
                     /gene="murJ"
                     /locus_tag="VG274_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWE05119.1"
                     /translation="MFRRLLSVGGFTLLSRITGFMRDILMAWMLGKGVLSDAFIVAFL
                     FPNYFRQIFGEGTINPAFLPRYAALRAKGEVRAAELFADRVFSWQMAAQLVILVLALV
                     FMPAIIHVLAPGFADNPGQLALTVQLARITFPYLILTLVAVQLSAMLNAIEKFWAAAA
                     WSNFLNLAMIAALLASHWFPNAAFAAAWGVLAGGVAQLVFMLWTARQDGLSLRIAWPR
                     WTPEIKEFFVALGAVTVGAASVVIAPFIDTVIASLLPTGTRTALYYADRINQLPLGVL
                     GIALGTVLLPEMSSRLALDDRSGSDDAQNRSAALSLLLTLPFAAVFVAIPDTIMRAIF
                     AHGAFDRNAAALSAIALAAYGVGLPAMALVRIFASTFYARHDTMTPARATVIAIAVNI
                     ALKFLFVWGLHLGIAGIALGTSIGAWLNVGLLAFLGHRRNLLQLSADFRRSLVPVVLA
                     GAATAIAAYAAVALGRHIVHAGGLHEVVLLCVAFVAGSLAYGAVVVLFRHRLPLGRFS
                     GVGAAP"
     gene            complement(2133..2786)
                     /locus_tag="VG274_00325"
     CDS             complement(2133..2786)
                     /locus_tag="VG274_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWE05120.1"
                     /translation="MNGARFKLTALVLLWFALGADAHAVNAPLRILAFGTSLTQSFGV
                     PPGMELPAVLQAKLDAAHIAAHIINAGVSGDTSADGLSRIDWSLADHPDAAMVELGSN
                     DALRGIDPVQTEQNISSVLEKLTAAHVPVLLLGMKAPRNLGREYAAVFDAIYPRLAAR
                     YHAILYPFVLDGVALDPKLNQADGIHPNPEGVKVIVVRLFPYVQKLIAEARHKKDAP"
     gene            3009..3695
                     /locus_tag="VG274_00330"
     CDS             3009..3695
                     /locus_tag="VG274_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE05121.1"
                     /translation="MSTPAIALDNVRLELGRAAGRVAVLRGVSLRIAAGESVALLGPS
                     GSGKSSLLMVAAGLEKPTAGRAILAGTDVSNLNEDALARLRRNTVGIVFQSFHLIPTM
                     TALENVALPLEFAREADAFQRAREELDAVGLAARAHHYPGQLSGGEQQRVALARAMVV
                     RPRVLFADEPTGNLDTETGAEVAELLFALPERTNATLFLVTHEETLARRCKRIVMLRD
                     GAIVADTASA"
     gene            3692..6364
                     /locus_tag="VG274_00335"
     CDS             3692..6364
                     /locus_tag="VG274_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWE05122.1"
                     /translation="MNPLSLAWRLARRELRSGLSGFRIFFASLVLGVAAIAGVGSIAD
                     ALLAGLAEHGRVLLGGDVAVELVHRPMTTAERAFVAARGSTSDTISMRAMAYANGANA
                     ARQLIELKAVDNLYPLYGSVGLSPAIDFQKALACTPTSAPISASQDLSWPERAGHPVG
                     SDGRSRLGGPVEPGHDKLLGFNSQNSTTACGAIVEQTLLDRLGVARGGRMRIGNQTFR
                     IAAVLADEPDRLSGGFSLGPHAIISTAALDRTGLVTTGSLIDYTTHVKLPANASIAAF
                     RREARARFPDAGWQIRDRDHAAPGTDRFIRQVSVFLTLVGLTALAVGGVGAGQAVRAF
                     IDRKREEIATLKALGADGNLIFLTFFLQAMAIAALASLSGLVLGAAIPFIVEHFYGAE
                     LPVPASFRIFPGPLALAFVFGMLSAAAFAVPPLARAREIAPAGLFRDLVAPAQVRGRL
                     PYLATAAGAALLIVALALAVSPSRAFTLWFLAGAAGGFVALRLAAVGLRFLLRRLPAP
                     SRPAPRLALANMTRPGSPTDSVIVALGLGLSLLAAVTLLDRTISAQVNDSLPESAPTF
                     FFVDIQPADAAAFDSTIQHFVSARDYRRTPMIRGRITALNHVPASKAKVASDSRWALN
                     GDRGITYAAAKPKDADLAAGRWWPANYRGPTLISFDAELAEGMGLKVGDSVTLNILGR
                     EIEGRIANLRHVDFSNGRQNFILILSPGIIDKAPHSFLATVRVAPRDEDALYRAVTDR
                     FRSVSTVRVRDVIAQLDTLLGRLAFGVRAASLVTILAGLLVLAGAIAAGQRARLYDST
                     VMKVVGATRSQIALVYGIEYALVGVLTGALALGAGTAAAWLVTRQVFDVHLAFDWRAV
                     LLTVAGGAVATLVFGFATSWTALAARPAAQLRNP"
     gene            6459..>6814
                     /locus_tag="VG274_00340"
     CDS             6459..>6814
                     /locus_tag="VG274_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HWE05123.1"
                     /translation="MADYDNRVLRGQTAAAGAIDAGLRAHMLRVYNYMLIGLALTGAT
                     AWLTAETSFGELFYRINPTTHTMGLTLLGMVALFAPLGLVLFMSFRIDRMSVGAAQGT
                     FWAYAALVGIGLAPILL"
BASE COUNT         1136 a         2280 c         2284 g         1114 t
ORIGIN      
        1 acgcgcaacg acgcctgctc aagcgagggt gaggggtggg ttacgcatcg cagcgctgct
       61 accccagggc atacgcgcgt tcggggcggt agaggctgcc gttgggggac aggatgctgg
      121 aatagaggat gaaggcgccg atctcgaacg ctccgctcac atagagcgca tgggcggcga
      181 ggaaggtgat gacgcggtcg cgcggggcgg cgcgcaatcg ggcgagcgtg acatgcggca
      241 cgaatttgcg ctcctcgggc ggcagcccga tgcgctgtac cgcgctttcg atcttgcgct
      301 gcaggtgcgc gacctgctcg ccatcgcgca cgcccgccca cagcgcgcgc ggattcctgc
      361 cgccgaactc gccgatgcct ttcagctcga gcgagaagcg ctgtgcgcgg atggtggcca
      421 gcgcgtcgtc gatcgcgatg gcgtcgcggc cgtccacctc gccgatgaag cgcaaggtca
      481 catgcagctg ttcgcgcgtc atccagcgcg cgccaggcac gccgccctgc agcgcgagga
      541 gagattcggc gacgggatcg ggcagcgcca gcgccacgaa cagccgcatc atggggctgc
      601 tcccacgccg ctgaatcggc cgagcggcaa tcggtggcga aacagaacca ccaccgcgcc
      661 gtaagcgagg ctgccggcga cgaaggcgac gcagagcagc acgacttcat gcagcccgcc
      721 cgcatgcact atgtgtcgtc caagtgcgac cgcggcgtaa gcggctatcg ccgtcgctgc
      781 acccgcaaga acaacgggaa ccagcgaacg ccggaaatcg gcactgagtt gcaacaggtt
      841 gcgccgatgg ccgaggaagg cgagcagtcc gacattcaac caggcgccga tcgacgtgcc
      901 gagcgcgatg ccggcgatgc cgagatgcag cccccacacg aacaggaatt tgagcgcgat
      961 gttcaccgcg atcgcgatca ccgtcgcgcg cgccggcgtc atcgtgtcgt ggcgcgcgta
     1021 gaacgtggag gcgaagatgc gcacgagcgc catcgccgga aggcccacgc cataggccgc
     1081 cagcgcgatc gccgaaagcg ccgcggcgtt cctgtcgaac gcgccgtggg cgaagatcgc
     1141 ccgcatgatc gtgtcgggaa tggcgacgaa cacggcggcg aacggcaagg tcagcaaaag
     1201 cgacagcgcg gcggagcggt tctgcgcatc gtcggatccg ctccgatcgt cgagcgccag
     1261 gcgcgacgac atctccggca gcagaacggt gccgagcgcg atgccgagca cgccgagcgg
     1321 caactggttg atgcggtccg cgtagtacag cgcggttcgc gtgcccgtcg gcaacaggct
     1381 cgcgatcacc gtgtcgatga agggtgcaat gaccacgctg gccgcaccga cggtcaccgc
     1441 accgagcgcc acgaagaact ccttgatctc cggagtccag cgcggccacg caatgcggag
     1501 cgaaagcccg tcctgccggg ccgtccacag catgaagacg agttgcgcga caccgcccgc
     1561 cagaacgccc cacgccgccg caaacgccgc gttggggaac cagtgcgaag ccagcagcgc
     1621 cgcgatcatc gcgaggttga gaaagttgga ccacgccgcg gcggcccaga atttctcgat
     1681 cgcgttcagc atcgccgaaa gctgcacggc gacgagcgtg agaatgagat agggaaaggt
     1741 gatgcgcgcg agctggacgg tgagcgcgag ttgccccgga ttgtcggcaa agccgggggc
     1801 aagaacatgg atgatcgcgg gcatgaagac gagcgcgagc acaagaatga cgagttgcgc
     1861 cgccatctgc caggagaaga cgcggtcggc gaacagctcc gccgcgcgaa cctcgccctt
     1921 tgcgcgcagc gccgcatagc gcggcaggaa ggcgggattg atcgttccct cgccgaaaat
     1981 ctggcggaaa taattgggaa acagaaatgc gacaatgaac gcgtccgaca gcacgccctt
     2041 gccgagcatc cacgccatga gaatgtcgcg catgaatccg gtgatgcgcg acagcaacgt
     2101 gaagccaccg acggagagca ggcgccggaa catcaaggcg cgtctttctt gtgccgtgct
     2161 tcggcgatca gcttttgcac atagggaaag agccgcacga cgatcacctt cacgccctct
     2221 ggattgggat ggatgccgtc ggcctggttg agcttcggat cgagcgccac gccatcgagt
     2281 acgaaaggat agaggatcgc atgatagcgc gcggcaaggc gcggatagat cgcgtcgaag
     2341 acggcggcgt attcccgccc gagattgcgc ggcgccttca tgccgagcag cagcacaggc
     2401 acgtgcgcgg cggtcaattt ttcgagaacg gaggagatgt tctgctccgt ctgcaccgga
     2461 tcgatgccgc gcagcgcatc gttcgaaccc agctcgacca ttgctgcgtc gggatgatcg
     2521 gcgagcgacc agtcgatgcg cgagagcccg tcggcggagg tgtcgcccga cacaccggca
     2581 ttgatgatgt gcgccgcgat gtgcgcagcg tcgagcttcg cctgcaggac ggccggcagc
     2641 tccattcctg gcggcacacc gaagctctgc gtcaggctgg tgccgaaggc gaggatcctg
     2701 agcggcgcgt tgacggcgtg agcgtcggcg ccgagcgcaa accaaagaag gacaagggcc
     2761 gtcagcttga atcgggcgcc gttcatgcca tatcgcctat cacgcccgcg gccatacggc
     2821 acgcgcgtcg attgatggaa agccatgacg cagctctcac ttaacctcct ccttgtgggg
     2881 aggtcgaaat cgcgtagcga tttcgggtgg gggtctgtgc gctgcgccgt accccccacc
     2941 cgaaaaacgc gaagggcgtt tttcgacctc cccacgaggg ggaggtagag actgattcgc
     3001 acccacggat gtccactccc gcgatcgctc tcgacaatgt gcggctggag ctggggcgtg
     3061 cggcgggacg tgttgcggtg ctgcgcggcg tctcgctccg catcgcagcg ggcgaatcgg
     3121 tggcgctgct ggggccttcg ggctcgggca aatcttcgct gctcatggtc gcggcgggac
     3181 tcgagaagcc gaccgcaggg cgcgccatcc ttgcgggaac ggatgtgtcg aacctcaacg
     3241 aggacgcgct cgcccggctg cgccgcaaca cggtcggcat cgtctttcag tccttccatc
     3301 ttatccccac gatgacggcg ctggagaacg tcgcgctgcc gctcgagttc gcgcgcgagg
     3361 ccgatgcgtt ccaacgcgcc agggaggaac tcgacgcggt gggactggcc gcgcgtgcgc
     3421 atcactatcc cggtcaattg tcgggcggcg agcagcagcg cgtggcgctg gcgcgcgcca
     3481 tggtggtgcg cccgcgcgtc ctgttcgccg acgagccgac cggcaatctc gatacggaaa
     3541 ccggcgcgga ggtggcggag cttctgtttg ctttgcccga gcgcaccaac gccacgctgt
     3601 ttctcgtcac ccatgaggag acgctggcgc gccgctgcaa gcgaatcgtg atgctcaggg
     3661 atggcgcgat cgtggccgac acggcgtctg catgaacccg ctttcgctcg cctggcggct
     3721 ggcgcgccgc gaattgcgca gcggactctc gggcttccgc atcttcttcg ccagtctggt
     3781 actcggcgtg gcggcaatcg ccggagtcgg ttcgattgcc gacgcgttgc tcgcgggact
     3841 cgccgagcac ggccgcgtgc tgctcggcgg cgacgtcgcg gtcgaactcg tgcaccggcc
     3901 gatgaccacg gccgaacgcg ccttcgttgc ggcgcgtggc agcacctccg atacgatctc
     3961 catgcgcgcg atggcctacg caaacggtgc gaacgccgcg cgccagctga tcgaattgaa
     4021 ggccgtcgac aatctctatc cgctctatgg cagcgtcggc ctctcgccgg cgatcgattt
     4081 tcaaaaggca cttgcctgca cgcctaccag cgctccgatt tcagcttccc aagacttgtc
     4141 atggcccgaa agagcgggcc acccagtcgg aagcgatggg cgttcgcgtc tgggtggccc
     4201 ggtcgagccg ggccatgaca agcttttggg cttcaattct cagaattcga ccactgcctg
     4261 cggggcgatt gtcgagcaga ctctgctcga ccggctgggc gtcgcgcgcg gcggtcgcat
     4321 gcgaatcgga aaccagacct tccgcatcgc cgccgtgctg gccgacgagc ccgacagact
     4381 ctccggcggc ttcagcctcg ggccgcacgc gatcatctcc acggccgcgc tcgatcgcac
     4441 gggactcgtc accaccggca gcctgatcga ctacaccacg catgtgaagc tgcccgcgaa
     4501 cgcttcgata gcagcgttcc ggcgcgaggc ccgggcgcgc tttcccgatg ccggctggca
     4561 gatccgcgac cgcgatcacg cagcacccgg caccgatcgt ttcatccggc aggtgagcgt
     4621 gtttctcacg cttgtcgggc tgacggcgct ggccgtcggc ggagtcggcg cgggccaggc
     4681 ggtgcgcgcc ttcatcgatc gcaagcgcga ggaaatcgcc acgctcaaag cgctcggcgc
     4741 cgacggcaat ctcatcttcc tgacgttctt cctgcaggcg atggcgatcg cggcgctcgc
     4801 ctcgctgagc ggcctcgttc tcggcgcggc gattccgttc atcgtcgaac atttttacgg
     4861 cgcggaactt ccggtcccgg caagtttcag gatctttccg ggacccctcg cgctcgcctt
     4921 cgtcttcggc atgctctccg ccgccgcgtt cgctgtcccg ccgctggcgc gcgcccgcga
     4981 gatcgcgccg gctggcctgt tccgcgatct cgttgcgccc gcgcaggtgc ggggccgcct
     5041 gccctatctc gccacggcgg ccggcgcggc gctgctcatc gttgcgctgg cgctcgccgt
     5101 ttcgccgtcg cgcgccttca ccctgtggtt tcttgccggc gccgcaggcg gattcgtcgc
     5161 gctgcgactc gcggctgtgg gcttgagatt cctgctgcgc agactgcccg ccccgtcgcg
     5221 tccggcgccg cgcctcgcgc tcgccaacat gacgcggccc ggctcgccga cagattcggt
     5281 catcgtggcg ctcgggctcg ggctttcgct tctcgcggcg gtgacgctgc tcgaccgcac
     5341 gatctccgcg caggtcaacg attccctccc cgaatccgcg ccgaccttct tcttcgtcga
     5401 catccaacct gcggacgcgg cagcgttcga tagcaccatc cagcacttcg tttccgcgcg
     5461 cgactaccgg cggacgccaa tgatccgtgg acggatcacc gcgctcaacc acgtgcccgc
     5521 ctccaaagcg aaggtcgcca gcgattcgcg ctgggcgctg aacggcgacc gcggcatcac
     5581 ctacgcagcc gcaaaaccga aagacgccga tctcgcggcg gggcggtggt ggccggccaa
     5641 ttacagggga ccgacgctca tctccttcga cgccgagctg gcggaaggca tgggcctcaa
     5701 ggtcggcgat tcggtaacgc tcaatatcct cggccgcgag atcgaaggac gcatcgccaa
     5761 tctgcggcac gtcgatttca gcaacgggcg gcagaatttc atcctcatcc tttcgcccgg
     5821 catcatcgac aaggcgccgc atagtttcct cgcgacggtg cgcgtcgccc cgcgtgacga
     5881 ggacgcactc taccgcgcgg tgaccgaccg ctttcgttcg gtctcgacgg tgcgggtgcg
     5941 cgacgtcatc gcgcagctcg acacgctcct cggccggctc gccttcgggg tgcgggcagc
     6001 gagccttgtc acgattctgg ccggcctgct cgtgctcgcc ggcgcgatcg cggcgggcca
     6061 gcgcgcccgg ctctatgatt cgacggtgat gaaagtggtg ggcgcgaccc gatcgcagat
     6121 cgcccttgtc tacggcatcg aatatgcgct cgtcggcgtg ctgaccggcg cgctcgcgct
     6181 gggcgcgggg acggccgccg cctggctggt gacacggcag gtcttcgatg tgcatctcgc
     6241 cttcgactgg cgcgcggttc ttctgaccgt ggcggggggt gcggtggcga ctctcgtctt
     6301 cggatttgcc acaagctgga cggcgctggc cgcgcggccc gcggcgcagt taagaaatcc
     6361 gtaattccgg cgctttcgcc cgcgccttga tccggcgggc cgcaagcccg atattatgcg
     6421 cgacccggtc cggattcgga cccaggagga attgccagat ggctgactac gacaaccgcg
     6481 tgctgcgcgg ccagacggcg gcggccggcg caatcgacgc cggactgcgc gcccacatgc
     6541 tgcgcgtcta caattacatg ctgatcgggc tggcgctgac cggcgccacg gcctggctga
     6601 ccgccgaaac ctcgttcggc gagctgttct accgcatcaa ccccaccacc cacacgatgg
     6661 gcctgaccct gctgggcatg gtcgcgctgt tcgcgccgct cgggctggtg ctgttcatgt
     6721 cgttccggat cgaccggatg agcgtgggcg cggcgcaagg aacgttctgg gcttatgcag
     6781 cgctggtcgg tatcggcctg gcgccgatcc tgct
//
LOCUS       DASZMC010000019         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1656148,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000019 DASZMC010000000
VERSION     DASZMC010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656148"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VG274_00345"
     CDS             <1..203
                     /locus_tag="VG274_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05124.1"
                     /translation="PPELLDRLRRAESIGRPLGDDRFIGKLEAMTRRLLKPGKRGPKP
                     RAEPDARQAEFSGLSVRQAKLT"
     gene            540..1945
                     /gene="ltrA"
                     /locus_tag="VG274_00350"
                     /pseudo
     CDS             540..1945
                     /gene="ltrA"
                     /locus_tag="VG274_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          387 a          597 c          680 g          281 t
ORIGIN      
        1 ggccgccgga attgctcgac cgtctgcgcc gcgccgagag catcggacgc cccctgggcg
       61 acgaccggtt tatcggcaag ctcgaggcca tgacccggcg cttgctcaag cccggaaagc
      121 gcgggccgaa gcctcgggcc gaaccggatg cccgacaagc ggagtttagc ggactgtcag
      181 tgcgtcaggc gaagctgacg tgatccagct ggtggaagtc cggcccgggt aaggggtagc
      241 tcccgcctgg tagcgagtgt tgcggggtcg tcggcgacgg cggctgcgaa gcgtacacag
      301 cgatcgtgtg gggtgggcta ttgaggcgcg aaagtcacaa atcgcagagg ccgaggccgt
      361 tgacagcgcc gaaggcaaca tgggcggcgc cgttactgcg aggcgctgtc gctctgccgt
      421 ggtcgaagac cccatcacgc acgaagggag cacgtcggaa cctgagaggt ctcgggtccg
      481 gccggacagc cgacggctgt cccggtccgc gccgggaagg cgatgagctt tagccggcga
      541 tgaacggacg cgagacgtca gaccctgcca tagtagcgat gaagccgacg aacgaagccg
      601 ggcgaccggt ggaggagccg gtggagcgaa gggcgggggc cgaggggaac gcggatcagc
      661 aaagcacgct ccggtctcag agccgggctg gcgtgaccca ggcgctggac cgcgtacggc
      721 aggcagcaaa gcaaaggaag aaggaacggt tcaccgcgct ccttcaccac atcaatccgg
      781 acaccttgcg cgcggcgttc tacgcgctga agcgcaaggc cgtccccggc gtcgatgggg
      841 tgagctggga ggactacgag gcagacctcg agctccggct tgaagacctg cacggtcggg
      901 tccaccgagg agcgtaccgg ccgcaaccgt cccgccggac gtacatacca aaggcggacg
      961 ggaagcaaag gccgctggcg attgccgccc tggaggacaa aatcgtccag ggcgcggtcg
     1021 tcatggtgct gaacgcgatc tacgaggagg acttcctcgg gttctcgtac gggttccggc
     1081 cggacgcgga ccgcacgacg cgctcgacgc cctgacggtc gggatcgcca gccggaaggt
     1141 gaactatatt ctggacgccg atatccgaga cttcttcggg tcggtgagcc aagagtggct
     1201 cgtccgtttc ctggaacatc ggatcggcga ccgacgcatc atccgcctga tccgaaaatg
     1261 gctccgggcg ggcatcctcg aagacggagt cgtgagcgtc aacgacaggg ggacggggca
     1321 aggttcggtg atctcaccgc tcctggccaa catctacctg cactacgtcc acgacctgtg
     1381 ggccgaacgc tggcgacggc atgaggctgc cggcgacatg atcatcgtgc gctacgccga
     1441 cgatctggtc gtgggattcg agcacgaggc cgacgcaggt cggttcctcg atgcgatgcg
     1501 tgcgcggttt gaggaattcg cgctatcgct ccatccggac aagacccgcc tgatcgagtt
     1561 cggccgcttc gcggcggcca aacgcgcgcg gcgcgggcag ggcaagccgg agaccttcaa
     1621 cttcctcgga ttcacccaca tctgcggaaa atcccgcgcc ggcaagttcc agatcaaaag
     1681 aaagagccga cgcgaccgca tgcaggcgaa gctcaaggaa gtgaaggcgg agctgcggcg
     1741 gcggatgcat gagccgatcc gcgaaacggc gggatggctc aagcagctcg tcaccggcta
     1801 tttccaatac cacgccgtgc caaccaacgg tgcggcgctc aaggcattcc gccatcatgt
     1861 catcgatctc tggcggcgca cgcttcggcg gcgcggtcag aagcaccaga tgacatggcc
     1921 gcggatcgga aagctggccg acgac
//
LOCUS       DASZMC010000020         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3036155,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000020 DASZMC010000000
VERSION     DASZMC010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036155"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VG274_00355"
     CDS             complement(<1..219)
                     /locus_tag="VG274_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05125.1"
                     /translation="MYDAIVFLPLVGALIAGLFGRVIGARASEIVTTVLLFVSAALSC
                     WAFYDVIFLGHSTLVGIMQWIASGNFKAD"
     gene            complement(223..531)
                     /gene="nuoK"
                     /locus_tag="VG274_00360"
     CDS             complement(223..531)
                     /gene="nuoK"
                     /locus_tag="VG274_00360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWE05126.1"
                     /translation="MAVGLANYLAVAAILFTVGVLGIFLNRRNVIIILMSIELILLSV
                     NINFVAFSAFLGNLTGQVFALFVLTVAAAEAAIGLAILVVYFRNRGTIAVETVNLMKG
                     "
     gene            complement(531..1136)
                     /locus_tag="VG274_00365"
     CDS             complement(531..1136)
                     /locus_tag="VG274_00365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005164.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWE05127.1"
                     /translation="MLTGIAFYLFSAVLIGAALMVIASRNPVHSVLFLILAFFNAAAL
                     FILLGAEFLALILLIVYVGAVAVLFLFVVMMLDIEFAELKRGALQYLPFGGLVGVILA
                     AELVLVGIGALVPQAGALVPHATPEHLTNTEALGRVLYTDYVYYFETAGLILLVAMIG
                     AIVLTLRHKPGVRRQKIALQNARDPRLAIELVDVQPGQGGP"
     gene            complement(1397..1885)
                     /gene="nuoI"
                     /locus_tag="VG274_00370"
     CDS             complement(1397..1885)
                     /gene="nuoI"
                     /locus_tag="VG274_00370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWE05128.1"
                     /translation="MAWLDRTARSIFFWELVKAFGLGMRYFFKPKATINYPFEKGPLS
                     PRFRGEHALRRYANGEERCIACKLCEAICPALAITIEAEPREDGSRRTTRYDIDMVKC
                     IYCGLCQESCPVDAIVEGPNFEFATETREELYFDKARLLANGDRWESEIAQNLELDAP
                     YR"
     gene            complement(1892..2926)
                     /gene="nuoH"
                     /locus_tag="VG274_00375"
     CDS             complement(1892..2926)
                     /gene="nuoH"
                     /locus_tag="VG274_00375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWE05129.1"
                     /translation="MIFTSAFQHLGLPYAWAWLISQLITMAIFAVSLLVMLAYILLAD
                     RKIWAAVQIRRGPNVVGAFGLLQSFADFFKFAFKEPVIPAGANKAIFLLAPLMTFTLA
                     FAAWAVVPLDNGWVIANINVGILYIFAISSLGVYGIIMGGWASNSKFPFLGGLRSAAQ
                     MVSYEVSIGFVIITVLLFAGSLNLSDIVRAQAHGWFAFSILFPMLVVFFVSALAETNR
                     PPFDLPEAESELVAGFMVEYSSTPYLMFMIGEYLNIVLMCAMITILFFGGWLPPFHVW
                     PFTAVPGVIWFLIKIWFFFFLFAMVKSIVPRYRYDQLMRLGWKVFLPTALLWVVVTAG
                     WVLVFRTHAM"
BASE COUNT          697 a          892 c          938 g          481 t
ORIGIN      
        1 gtcggccttg aaatttcccg acgcgatcca ctgcatgatg cctacgagcg tggaatggcc
       61 caggaaaatc acgtcgtaga acgcccagca ggatagcgcc gcggagacga agaggaggac
      121 cgtagtcacg atctccgacg cgcgcgcgcc gatgacgcgt ccgaacagac cggcgatcag
      181 cgcgcccaca agcggaagaa acacgatcgc gtcgtacacg cctcagccct tcatcagatt
      241 gacggtctcg accgcgatgg tgccgcggtt gcggaaatag acgacgagga tggcgagccc
      301 gatcgcagct tccgccgcgg cgacggtgag gacgaaaagc gcaaagacct ggccggtgag
      361 attgccgaga aaggcggaga aggcgacgaa attgatgttg accgagagga ggatcagctc
      421 gatcgacatc aggatgatga tgacgttccg gcggttcagg aatattccga gcacgccgac
      481 cgtgaagagg atcgcagcga cggcgagata gttggcgagg ccgacggcca tcacgggccg
      541 ccctgccccg gctgcacgtc gacgagctcg atggcgagcc tggggtcgcg cgcgttctgc
      601 agcgcgatct tctgccggcg cacgcccggc ttgtggcgca aggtgagcac gatcgcgccg
      661 atcatcgcga cgagtaggat cagcccggcc gtttcgaagt aatagacata atccgtgtag
      721 agaactcgcc ctagcgcctc ggtattggtg agatgctcag gcgttgcgtg cggcacaagc
      781 gcaccggctt gcggcacaag cgcccctatg cccaccagca cgagttcggc ggcaaggatc
      841 acgccgacga ggccgccgaa gggcaggtat tgcagcgcgc cgcgtttcag ttcggcaaat
      901 tcgatgtcga gcatcatcac gacgaagagg aacagcaccg ccaccgcgcc gacatagacg
      961 atgagcagga taagcgcgag gaactccgcg cccagcagaa tgaacagcgc tgcggcgttg
     1021 aaaaaggcaa ggatcaggaa cagcaccgaa tgcacggggt tgcgcgaagc aatcaccatc
     1081 agcgccgccc cgatcagcac cgccgagaac agataaaagg caatccccgt caacatgcct
     1141 gagtgttcat ctctgttgat atccgcgcgc taacaaaaag cgtgtcatcc cggccgaggc
     1201 gccagccgac agccgggacc caggaccgtg aagaaattcc gtaagcactg cttctgggtt
     1261 cccgctttcg cgggaatgac acgcgtgggg acaagtggac tcttaaactt tgcctcaccg
     1321 cgcgcgaaat tgttttcccg cgtcccctgc gcatcccttg cgtcctctgg gttaaacatt
     1381 ttctgtgttt ctcgcactag cgatacgggg catcgagttc gagattttgc gcgatctcgc
     1441 tttcccagcg atcgccattg gcgagcaggc gcgccttgtc gaagtagagt tcctcgcgcg
     1501 tctcagtggc gaattcgaaa ttcggccctt ccacgatcgc gtccaccggg caggattcct
     1561 ggcacagccc gcagtagatg cacttgacca tgtcgatatc gtagcgcgtg gtccgtcggc
     1621 tgccgtcctc gcggggctcc gcttcgatgg taatggcgag ggcgggacaa atcgcttcac
     1681 agagcttgca ggcgatgcag cgttcttccc cattggcgta gcgacgcaac gcgtgctcgc
     1741 cgcgaaagcg cgggctgagc gggcccttct cgaagggata gttgatcgtc gccttcggct
     1801 tgaagaaata gcgcatgccg aggccgaatg ccttcaccag ctcccagaag aagatggaac
     1861 gcgcggtgcg gtcgagccag gccatgaaac ctcacatcgc gtgggtgcgg aagacgagca
     1921 cccagcccgc cgtgaccact acccacagaa gggccgtcgg aaggaagacc ttccagccga
     1981 gccgcatcag ctggtcgtag cgatagcgcg gcacgatcga tttcaccatc gcgaacagga
     2041 agaagaagaa ccagatcttg atgaggaacc agatcacgcc cggcaccgcg gtgaatggcc
     2101 acacatggaa tggcggcagc cagccgccga agaacaggat cgtgatcatc gcgcacatga
     2161 gcacgatgtt cagatactcg ccgatcatga acataagata aggcgtcgag gagtattcga
     2221 ccatgaaccc ggccacgagt tcggattccg cttccggcag gtcgaatggt ggccgattgg
     2281 tttcggcaag cgccgagacg aagaacacga ccagcatcgg aaacaggatc gagaatgcga
     2341 accagccatg cgcctgggcg cgcacgatat cggagagatt gagcgagccg gcgaagagca
     2401 ggaccgtgat gatcacgaag ccaatcgaga cctcgtagga caccatctgc gccgcggaac
     2461 gcaacccgcc gaggaacggg aatttcgagt tcgacgccca gccgcccatg atgatgccgt
     2521 aaacgccgag cgacgaaata gcgaaaatat agaggatgcc gacgttgata ttggcgatca
     2581 cccagccatt gtcgagcggc accacggccc acgccgcgaa ggcgagcgtg aaggtcataa
     2641 gcggcgcgag gaggaagatc gccttgttcg cgcccgccgg gatcaccggc tccttgaaag
     2701 cgaatttgaa gaaatcggca aaggattgaa gcaggccgaa cgcgcccacg acgttgggtc
     2761 cgcggcggat ctgcaccgcc gcccagatct tgcggtcggc aagaaggatg taggccagca
     2821 tgaccagcag cgacaccgcg aagatcgcca tggtgatcaa ctggctgatc agccacgccc
     2881 acgcataggg caaaccgaga tgctggaatg cagaggtgaa gatcatcgca ctattccgcc
     2941 gccattttct gcccgccgct gacgaagatg cggctgcatt cggccatggt ggggcttgcc
     3001 cgcgcgat
//
LOCUS       DASZMC010000021         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2206623,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000021 DASZMC010000000
VERSION     DASZMC010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206623"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VG274_00380"
     CDS             complement(<1..255)
                     /locus_tag="VG274_00380"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HWE05130.1"
                     /translation="MPTMLLEAGESDSAPQADRSPGVGWQSVIDLHDCRTPHLDDIRW
                     VRQTMLEAAARAEATVIGEHFHRFTPHGISGVVVIGESHLA"
     gene            318..1064
                     /locus_tag="VG274_00385"
     CDS             318..1064
                     /locus_tag="VG274_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWE05131.1"
                     /translation="MRIAHNFCYGQEAGAGQARYDEAIHESAGLPFMQRKDGVRAIER
                     AVVQIAHNLGRRDLGRQVARKLGKTVDLSHLLVVDAIDDQSAENGVPPTIGDIARYLD
                     VHHSRASRMVKGAIRATLATRLASQADGRKSCVELSDRGREIAAAIRDARARYFAVRM
                     KDWPNADRREFARLLIQFARSDHLRRGDQGDGGVSVSESPSAQAGTIDDLLGNSQGPA
                     AMPAVESKRQRRRKKQEATAVRRRRIVSSE"
     gene            1061..3004
                     /locus_tag="VG274_00390"
     CDS             1061..3004
                     /locus_tag="VG274_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HWE05132.1"
                     /translation="MNRSSRDGESVPEQAGSAAAAPSLRAGHAPGHSAALTLGALGVV
                     FGDIGTSPIYAMRETVRATGGSLPNQFAVMGALSAIVWALILVVTIKYVILIMRADND
                     GEGGVLALAGLAHRSPRLPRRVKNMIGIAALLGLALFFGDGMLTPAISVLSAVEGLGI
                     ETESLQPLILPLTLVILVGLFAMQSRGTAKIGRLFGPIMVVWFVVLAILGLRGIVLAP
                     RILLAVNPFYAIMLFVHAPWTAFVALGAVVLAVTGCEALYADMGHFGKQPIRNAWLYF
                     VLPALLINYLGQGAELLHNPAVAGIPFYALAPHWAHYPLVLLATVATIIASQAVISGV
                     FSLARQAVQLGRLPRMEIRHTSATDYGQIFVPRMNAILCIGVVLIVLIFHTSSSLAAA
                     YGIAVSGVMVIDTFNASIVATRQWHWSIRAALALFGLLGLIDLSFLAANSLKIVQGGW
                     LPLLIAGCVFLVMNIWRIGRRAHLEKLRDEALPMDLFLQRADKTPQRVAGTAVFLSTR
                     SDMVPGALLHSLKHYKVLHERVVITHVLVEEVPIVPASRRVDVRKLGKGFFGVTIRHG
                     FFETPDIPRILEMARVFGLAIDIATTTFFIGRETLVPSEHPALGYWATLIYLRLAASA
                     LSPARFYHLPPNRVVEFGTQIAL"
     gene            complement(3001..4032)
                     /locus_tag="VG274_00395"
     CDS             complement(3001..4032)
                     /locus_tag="VG274_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HWE05133.1"
                     /translation="MKTFYSAAHLEHAPANEFEGGRLTPMPEVPARAEAVRARIEERA
                     LGPVVAPTHFGLEPILRVHSREFADFLVSAHADWTKKYGPEVAEAVPSAWPARGLRPN
                     RAGDIEARLGSYAFDTATPIVRGTWEAARSAVDVALSAAQCIRDGARAAFALTRPPGH
                     HAASELFGGYCYLNNVAIAAQWFADSGARCAILDVDYHHGNGTQAIFYSRADVFYCSL
                     HADPAFAFPHFSGFADERGEGGGQGANLNLPLPSRTEFQAYEQALTCAREAIMGSGAD
                     ILLVSLGLDTFENDPISDFRLKSGDYLKMGDAIARLNKRTLFVFEGGYDLATLGLNTV
                     NVLEGFEQG"
BASE COUNT          733 a         1320 c         1324 g          821 t
ORIGIN      
        1 ggccagatgc gattcgccga tgacgaccac gcccgagatg ccatggggcg tgaaacggtg
       61 gaaatgttcc cctatgacgg tcgcttcggc gcgggccgca gcttcaagca tcgtctgtcg
      121 cacccagcgg atatcgtcga ggtgcggcgt ccggcagtca tgcaggtcga tcacggattg
      181 ccagccgacg cccgggctcc gatcggcttg cggcgcgctg tcgctttccc cggcttccag
      241 cagcatcgtc ggcattcccc gcccgcaaac accgcggcat ccaataccgc ggcattttat
      301 gtgctctatg catacatatg cgaatagcac ataatttctg ctacggtcaa gaggcaggag
      361 ccggacaggc gcgctatgat gaggcaattc acgaatcggc cggtctgccc ttcatgcagc
      421 gaaaagacgg agttcgcgca atcgagcgcg cagtggttca aatcgcgcac aatcttggcc
      481 gccgcgattt ggggcggcaa gtcgcacgca agctcggaaa gacggtcgat ctgtcgcatc
      541 ttcttgtcgt ggatgcgatc gacgaccaga gtgctgagaa tggagtccct ccgacgatcg
      601 gggacatcgc aaggtacctc gacgtccacc actcgcgcgc gagccggatg gtcaagggcg
      661 cgattcgggc gacgctggcg acgcgtcttg cttcgcaggc ggatgggcgc aagtcttgtg
      721 tggagctcag cgaccgggga cgcgagattg cggctgcgat tcgcgacgct cgcgccaggt
      781 atttcgcggt gcgcatgaaa gattggccga atgccgaccg cagggaattt gcacgattgc
      841 tcattcaatt cgcgcggagc gaccatttgc ggcgcggcga tcagggcgac ggcggtgtct
      901 ctgtttcaga atctccatcc gcgcaggcgg ggaccattga cgatcttctc gggaattcgc
      961 aaggacccgc cgcaatgccc gcagtggaaa gcaagagaca acgcaggcgc aagaaacagg
     1021 aagcaaccgc cgtgcgaagg cggcgaatcg tgagttccga atgaaccgtt cgtcacgcga
     1081 cggggaatcc gtacccgaac aagccggaag tgcggccgct gcgccgtcgt tgcgcgccgg
     1141 ccacgcgccc ggccacagcg ccgcgctgac gctgggggcg ctcggcgtcg tattcggcga
     1201 tatcggcaca agtcccatct acgcgatgcg cgaaaccgtg cgcgcgaccg gcggcagcct
     1261 tcccaatcag tttgcggtga tgggcgcgct gtcggcgatc gtctgggcgc tgatactcgt
     1321 ggtcacgatc aagtacgtca tcctcatcat gcgcgccgac aatgacggcg agggcggtgt
     1381 tcttgcactc gcgggtctcg cccatcgttc gccgcgcctg ccgcgccggg tcaagaacat
     1441 gatcggcatc gcggcgcttc tcgggctggc tctgtttttc ggcgacggga tgctgacacc
     1501 ggcgatttcg gtcctgagcg ccgttgaggg tctggggatc gagacggaaa gcctgcagcc
     1561 gctgatcctt ccgctgacgc tggtgatcct ggtcggcctg ttcgcgatgc agagtagggg
     1621 aacggcgaag attggcaggc tctttggccc gatcatggtc gtgtggttcg tcgtcctggc
     1681 gatccttggc ctgcgcggga tagtgctcgc gccacgcatc ctgcttgcgg tcaatccgtt
     1741 ctacgccatc atgctgttcg tgcacgcgcc gtggacggca ttcgttgcgt tgggcgcggt
     1801 cgtgcttgcg gtgacgggat gcgaagccct ctacgccgac atgggacatt tcggcaagca
     1861 gcccatccgc aacgcctggc tgtatttcgt gttgcccgcg ctgctgatca actatctggg
     1921 tcagggcgcg gagctcttgc acaatccggc ggttgcgggg attcccttct acgcgctcgc
     1981 gccgcattgg gcgcattatc cgctggtgct gctcgcaact gtcgccacga ttattgcctc
     2041 ccaggcggtg atatcgggcg tcttttcgct agcgcgccag gccgttcagc tcggccggct
     2101 gccacgcatg gagattcgcc acacctcggc gacggattac ggtcagattt tcgtgccgcg
     2161 aatgaacgcc attctctgca tcggcgtcgt gctgattgtc ctcatcttcc acacgtcgag
     2221 ttctctcgcc gcggcctatg gcatcgcggt aagcggggta atggtgatcg acacgttcaa
     2281 cgcgtcgatc gtcgcgaccc ggcaatggca ttggagcatt cgcgcggcgc tggcgctctt
     2341 cggcctgctg ggtctgatag atctttcatt cctggccgcg aattcgctga agatcgtgca
     2401 gggcggctgg ctgcccctgc tcattgccgg ctgtgtattc ctggtcatga acatctggcg
     2461 catcggacgg cgcgcccatc tcgagaaatt gcgcgacgaa gccttgccca tggacctgtt
     2521 tctgcagcgc gccgacaaga cgccgcaacg tgtcgccggc acggccgtat tcctcagcac
     2581 gcgtagcgac atggttcccg gcgcgctgct ccacagcctc aagcactaca aggtcctgca
     2641 cgagcgcgtg gtcataaccc atgtcctggt ggaagaagtg ccgatcgttc ccgccagccg
     2701 gcgcgtggac gtgcgcaagc tcggcaaggg tttcttcggc gtgacgatcc ggcatggatt
     2761 cttcgagacg ccggacatcc cgcgaattct cgaaatggcc cgcgtctttg gcctggcgat
     2821 cgacatcgcc accaccacct tttttatcgg gcgcgaaacg ctcgtgccgt ccgagcatcc
     2881 cgcgctcggc tactgggcga ccttgatcta tttgcgactg gccgcctcgg ctctttcgcc
     2941 ggcgcgattt tatcatctcc cgcccaaccg ggtggtcgag ttcggcaccc agatcgctct
     3001 ctaaccctgc tcgaatcctt cgagaacgtt gacggtgttc agcccaagcg tcgcaaggtc
     3061 gtagccgccc tcgaagacga agagcgtccg cttgttcaac cgcgcgatgg cgtcgcccat
     3121 tttcagatag tcgccgctct tgagccggaa gtccgaaatc ggatcgtttt caaatgtatc
     3181 gaggccgagc gacacgagca gtatgtctgc gccggacccc atgatggctt cgcgggcgca
     3241 ggtcagtgct tgctcgtagg cctggaattc ggtgcgggaa ggcagcggca gattgagatt
     3301 cgcgccttga cccccgcctt cgccgcgctc gtcggcaaat ccggagaaat ggggaaaggc
     3361 gaaggcggga tccgcatgaa gggaacagta gaagacatcg gcgcgcgaat agaaaatcgc
     3421 ctgcgtgcca ttgccgtgat gatagtcgac gtcgagaatt gcgcagcgtg cgcccgaatc
     3481 ggcgaaccat tgtgcggcga tggccacatt gttgagatag cagtagccgc cgaacagctc
     3541 actggccgca tggtgtcccg gcggccttgt cagggcgaaa gcggctcgcg cgccgtcacg
     3601 gatgcattgc gcagcggaca acgccacgtc gacggcggag cgtgccgcct cccaggttcc
     3661 tcggacgatg ggtgttgccg tgtcaaacgc ataactgccc agccgcgcct cgatgtcgcc
     3721 cgcccgattg ggacgcagac cgcgcgcggg ccatgccgag ggcaccgcct cggcaacctc
     3781 gggaccatat ttctttgtcc agtccgcatg ggcgctgacg aggaaatccg caaattcccg
     3841 gctgtgcacg cgcaggatcg gttccagtcc gaaatgcgtc ggcgcgacaa ctggcccgag
     3901 cgcccgctcc tctatgcgtg cacgcaccgc ttccgcgcgc gcaggcacct ccggcatcgg
     3961 cgtcagccgc ccgccttcga actcgtttgc cggcgcgtgt tcgagatgcg cagccgaata
     4021 aaacgtcttc atctcagccg agctgttcca actgcgcagc gctggcaatc gaggcaatgg
     4081 cagaccggga tccgtaccga tccgcccgcg caggtgcgcg cgattgtcca cagcagcacg
     4141 ccgcatcatg cttcgtcata tcgtctgttc cggaaggcgt tcgcgaaaat cagcatcc
//
LOCUS       DASZMC010000022         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_833363,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000022 DASZMC010000000
VERSION     DASZMC010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833363"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..489)
                     /locus_tag="VG274_00400"
     CDS             complement(100..489)
                     /locus_tag="VG274_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05134.1"
                     /translation="MRWRRGPRFGARAPAHPPNLVREYDLVALAGAALAAVSPKFPSS
                     NAAFWDHVNIAKELHHISRVIVLDHRDCGAYKVAFGKDFATGHDAETAQHKDVMLHVK
                     AKLATTHPHLGAEFYLMALDGKAERIV"
     gene            606..1124
                     /locus_tag="VG274_00405"
     CDS             606..1124
                     /locus_tag="VG274_00405"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWE05135.1"
                     /translation="MRRRDESPGLSQSAYPPVTYDLTLSFAHTALVKAFAYWNSSRGE
                     RAMPTRAAITPQGMRSFLPHVALVEVKPGPGGEPDYFVRLAGTAIEEVLGKRTGHSLT
                     QDLPPALAQRWRAQFDAVLKSRQPLRATGRIAYDNKTWLEAEILYAPLSEDGTDVSMV
                     FLAFAATVPKPG"
     gene            1238..1438
                     /locus_tag="VG274_00410"
     CDS             1238..1438
                     /locus_tag="VG274_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWE05136.1"
                     /translation="MLRVEVDRESDGRWIAEVPALPGVLAYGATEEEARARTEALALR
                     VIADRLDHGEPVPNEVRAVFAA"
     gene            complement(1972..2358)
                     /locus_tag="VG274_00415"
     CDS             complement(1972..2358)
                     /locus_tag="VG274_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE05137.1"
                     /translation="MAGIDVRPIRTEADYSWALREIERYFVNEPRRGTKAAARFDILA
                     ALIESYEVRNWRIEPPDPVEAIRYRMQQAGLSQADFARLIGSRSRASEILNRKRPLTM
                     EQAWKLHKEWHIPADALLRPNLAARS"
     gene            complement(2358..>2726)
                     /locus_tag="VG274_00420"
     CDS             complement(2358..>2726)
                     /locus_tag="VG274_00420"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HWE05138.1"
                     /translation="LRPGSPRGERHPSCSRIWNTLYRLMQIIARRTLKRFWSKHPPAE
                     QPLRNWFAMVDKAQWKSPGDAKAMFGGNVDFVGDNRLIFDIGGNKYRLVAHVSYLYKR
                     VLIKFVGTHKAYDRIDPERV"
BASE COUNT          497 a          860 c          850 g          519 t
ORIGIN      
        1 tatcatctca ccttcctcgt cccgctcatc ccgctcaccg tcctcggcgc gagcctcgcg
       61 cgacgcggac cgatggccga gcacccttag cgggcgccat caaacaatcc gctctgcttt
      121 cccgtccagc gccatgaggt agaattccgc gccaaggtgg gggtgagtgg tagcgagttt
      181 cgccttcaca tgcagcatta cgtccttgtg ctgcgcagtt tccgcgtcat ggcccgtggc
      241 gaaatccttg ccgaacgcca ccttgtaggc gccgcaatcg cggtgatcga gaacgatcac
      301 ccggcttata tggtgaagct ctttggcgat gttgacgtga tcccagaagg ccgcgttcga
      361 ggacggaaat ttcggcgaca cggcggcgag cgcggcgccg gccagcgcca cgagatcgta
      421 ttcgcgcacg aggtttggag gatgtgcagg tgccctggcg ccgaagcggg gaccccggcg
      481 ccaacgcacg tcgctatccc cggatttccg ctccggcggg aatcacaagt cgggtttgtg
      541 attcaatttg atcggaaagc gcactaaatc gtgtatgctg acatgtcacg cgaaggggtt
      601 cggccatgcg ccgccgcgat gaatcgcccg ggctttcgca atccgcctat ccgcccgtca
      661 cctacgacct gacgctcagc ttcgcgcaca cggcgctggt gaaagccttc gcctactgga
      721 actccagccg cggcgaacgc gcgatgccaa cgcgcgcggc tattacgccg cagggcatgc
      781 gcagctttct gcctcatgtg gcgctggtcg aggtgaagcc gggccccggt ggcgagccgg
      841 attatttcgt gcgcctggcg ggcacggcca tcgaggaggt gctgggaaaa cgcaccggcc
      901 attcgctcac gcaggacctg ccgcccgcgc tggcgcaacg ctggcgcgcg caattcgatg
      961 cggtgctcaa gtcccgccag ccgctgcgcg ccaccggccg catcgcctac gacaacaaga
     1021 cctggctcga agcggagatt ctctacgcgc cgctcagcga agacggaacg gatgtgagca
     1081 tggtcttcct cgccttcgcc gctacggtgc cgaagccggg gtaagggtgc ggcgtttata
     1141 ttgttttccg gccgtgcttc cgggccaatg aggcttgtct ggacgaaagg ctggcctgcc
     1201 tctacaagct caaacctgat tcaaggcaag atatgtcatg ctccgagttg aagtagatcg
     1261 cgagagcgat ggacgctgga ttgccgaagt gccggcgctt ccgggcgtgc ttgcatacgg
     1321 ggcgaccgaa gaagaggcga gggccaggac cgaggcgctt gctctgcgcg tgatcgccga
     1381 tcggctcgat cacggcgaac ccgtgccgaa cgaggtgcgg gccgttttcg ctgcgtgagc
     1441 gattggcctt cgaccaaagc gcggcaagtg ctgcgggcgc tcgtcaaaat cggctggacg
     1501 gtcaagaggc aaaccggttc tcatcgcacc ctggcgcgga ccggatggcc ggattttgcc
     1561 ttccatgaag gcgtggaccg agaatgcttg cgcgcctagc caaacgcacc ggcctcacgc
     1621 ccacggatct ctgatctgcg aatgcagtga ccacgtgcgt aactcgcgcc acagcgcatg
     1681 cgcggtttcc tgccgcgctg atcgaggtga agccgaggcc gcgggccgag ccgccaccgt
     1741 gccgaagccg cggtgagggt gtaattcagg acctgctcac ccttttcctc cccctccggt
     1801 ttgcttcgct tcgctgcgca aaccacctcc cccgcgatgg gggaggaaaa tcaagcgcgc
     1861 agcatggctg gatttccagg ccgaacagct cgggcgcatt catgaaaaat tgtgtgctca
     1921 cgttagtccc acgttggggg aggaaagatg cagctctggc cacgtctcat tttaactgcg
     1981 cgccgccaga ttcgggcgaa gcagcgcatc ggcaggaatg tgccattcct tgtgcagctt
     2041 ccaggcctgt tccatcgtca gaggccgctt gcggttgaga atctcagaag cgcgcgaacg
     2101 cgagccgatg aggcgcgcaa agtcggcctg gctcaatccc gcctgctgca tgcgatagcg
     2161 aatggcctcg acgggatcgg gcggctcgat ccgccaattc ctgacttcgt agctttcgat
     2221 cagcgcggcg agaatgtcga aacgcgccgc cgctttcgtg ccgcgccgcg gctcgttgac
     2281 gaaatagcgc tcgatctcgc gaagcgccca actataatcg gcctcggtcc tgatcggcct
     2341 gacgtcgatg cctgccatca cacgcgctcc ggatcgattc ggtcgtatgc cttgtgcgtg
     2401 ccgacgaatt tgatcagcac gcgcttgtac agataagaga catgggcgac gagccggtat
     2461 ttgtttccgc cgatatcgaa gatgagccgg ttgtcgccca cgaaatccac attgcctcca
     2521 aacatcgcct tcgcatcgcc agggcttttc cattgcgcct tgtcgaccat cgcaaaccag
     2581 ttccgcaggg gctgttccgc cggcggatgc ttcgaccaga aacgcttcag cgtccgcctg
     2641 gcgatgatct gcatgagacg atatagcgtg ttccatattc gggaacaaga ggggtgccgt
     2701 tcgccgcgcg gggagcccgg ccgaag
//
LOCUS       DASZMC010000023         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3039639,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000023 DASZMC010000000
VERSION     DASZMC010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039639"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VG274_00425"
     CDS             complement(<1..851)
                     /locus_tag="VG274_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05139.1"
                     /translation="MLDTGRLSVAGIGASNNPLLQAIWNAVAAYNSMAGAAGGGAQIK
                     PRADALDKVAFTVGAYLTSKPPQPKMKNRKRWNAMRTLAEQIGPEAARLGIRLLKGPS
                     DYTKIGPGHADQSYWLERLDPQHRPGYVLSGQWEGWLNDPASIAGKRSFWDKIGTVLN
                     PSANQQEVKYYPLIDPDELSQSRVSFKEELLWDDYFDAPVDTEGMETHFSGKGWGIFV
                     CSPDGKLFIHSHQAGVHHHSSFLAGGAVLAAGEIVVRDGVVMVITPKSGHYQPTPDNI
                     LNFVRKFR"
     gene            complement(922..>1735)
                     /locus_tag="VG274_00430"
     CDS             complement(922..>1735)
                     /locus_tag="VG274_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWE05140.1"
                     /translation="GKPIGVFMLAGPSGVGKTETALALAEALYGDEENVITINMSEFQ
                     EAHTVSTLKGSPPGYVGYGEGGVLTEAVRRRPYSIVLLDEVEKAHPDVHEIFFQVFDK
                     GWMEDGEGRFIDFKNTIILLTTNAGTDLIANMCKDPELMPDVESIAKALRAPLLKVFP
                     PALLGRIVTIPYYPLSDKMLKQIIRLQLGRIQKRVKENHKVPLQFDDKVIDLVASRCT
                     EVESGGRMVDAILTQSLLPAMSREFLNRLMEGRPLSRVEVTVENKDFAYKFV"
BASE COUNT          310 a          546 c          515 g          364 t
ORIGIN      
        1 cggaactttc tgacgaaatt caggatattg tcaggggtcg gctggtaatg cccgctcttg
       61 ggagtgatga ccataacgac gccgtcccgc acgacaatct ccccggccgc caggacggcg
      121 ccgccggcca ggaaactgga atgatgatgg acgccagcct ggtgactgtg aatgaacagc
      181 ttgccgtcgg gactgcacac gaaaatgccc cagcccttgc cgctgaagtg cgtctccatc
      241 ccctcggtat cgaccggcgc atcaaagtaa tcgtcccaga gaagctcttc tttgaacgac
      301 acacgggact ggctgagctc gtccggatcg atcagtggat aatacttgac ctcctgctga
      361 ttggcggacg gattcagcac cgtcccgatc ttgtcccaga atgatctctt cccggcgatg
      421 cttgccgggt cattcagcca cccctcccac tgccccgaaa ggacgtagcc cgggcggtgc
      481 tgcggatcga ggcgctcgag ccaataactc tggtcggcgt ggccggggcc gatcttggtg
      541 tagtcgctgg gccccttgag aagccggatg cccagtcgag cggcttcagg gccgatctgc
      601 tcggccagcg tgcgcatggc attccatcgc ttgcggttct tcattttcgg ctgcggaggt
      661 ttgctggtga gataggcgcc aacggtgaag gcgactttgt ccagggcgtc ggcgcgcggc
      721 ttgatctgcg cgccgccacc agccgctccg gccatgctgt tgtaggcggc cacggcattc
      781 cagatcgcct ggagcagggg attattactg gcgccgatcc cggcaactga aagtcggcct
      841 gtgtccagca ttcacttctc ctgttggttg acttcaatgc ggcggtgcgc gcgtccggac
      901 cgttcctgca cagccgccgc ctcaaacaaa cttgtacgca aaatccttgt tctccaccgt
      961 cacctccacg cgcgagagtg gcctgccctc catgagacgg ttgaggaact cgcggctcat
     1021 cgcaggcagg agcgactgcg tcaggatcgc atccaccatg cgcccgccgc tctccacttc
     1081 ggtgcaccgg ctggccacca agtcgatcac cttgtcatcg aactggagcg gcaccttgtg
     1141 attctccttc acccgcttct ggatcctccc cagttgcagg cggatgatct gctttagcat
     1201 cttgtccgac agcgggtaat acggaatcgt gacgatccgg cccagcagcg cgggcgggaa
     1261 gaccttcagc agcggcgcgc gaagcgcctt ggcgatgctt tccacgtccg gcatcagctc
     1321 cgggtccttg cacatgttgg caatcaggtc ggtgccggca ttggtggtca gcagtataat
     1381 tgtattttta aaatcgatga accggccttc gccgtcctcc atccagcctt tgtcgaagac
     1441 ctggaagaag atttcgtgaa cgtccggatg ggctttctcg acttcgtcga gcagcacgat
     1501 cgaatagggc cggcggcgaa ccgcctcggt cagcactccg ccctcgccat atccgacata
     1561 tcccggcgga gagcccttga gcgtggagac ggtgtgggcc tcctgaaatt cgctcatgtt
     1621 gatggtgatg acgttctctt cgtcgccgta aagggcttcg gccagcgcca gcgccgtctc
     1681 ggttttgccc acgcccgaag gtccggcgag catgaagacg ccgatcggct tgccg
//
LOCUS       DASZMC010000024         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4406884,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000024 DASZMC010000000
VERSION     DASZMC010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4406884"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VG274_00435"
     CDS             <1..572
                     /locus_tag="VG274_00435"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWE05141.1"
                     /translation="SIVTDRIETFTEKGLKLASGTELEADVIVTATGLVMELLGGMEV
                     SVDGQAIDFSKTLNYRGAMYSGVPNLASAFGYTNASWTLKCDLTCEYVCRLLNRMDRQ
                     GLRQCTPRNRDPSMPTEPWLDFSSGYVQRAMARFPRQGTRRPWKLYQNYALDIVSLRY
                     GKMEDGVLEFSNPAPAVSNHGTERLAGKA"
     gene            587..913
                     /locus_tag="VG274_00440"
     CDS             587..913
                     /locus_tag="VG274_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWE05142.1"
                     /translation="MRDRIAVQFPVHGWKPMSVVTYSYARQNLAKLMDDVERDRAPVY
                     ISRQRKRGAAVLMSLDDYQAMETTLYLLSNPKNAKLLREGIAELDAGKGVRHDIVPVR
                     RRARAG"
     gene            910..1188
                     /locus_tag="VG274_00445"
     CDS             910..1188
                     /locus_tag="VG274_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HWE05143.1"
                     /translation="MNLFWSSGAWDDYVEFQDRDPAMARKINDLIADVRRSPFQGLGK
                     PEPLRGPLAGWWSRRINQEHRLVYRVVGTRGRNQRVEILQCRFHYDRR"
     gene            complement(1562..1849)
                     /locus_tag="VG274_00450"
     CDS             complement(1562..1849)
                     /locus_tag="VG274_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWE05144.1"
                     /translation="MARTPIHPGEHLAEALDELGLSAAEFARQIDVPTNRITGVLRGQ
                     RAITADTALRFGHWFGMSAEFWLNLQKQYELRLAEKEKGVEIRRLPRRKAA"
     gene            complement(1858..2142)
                     /locus_tag="VG274_00455"
     CDS             complement(1858..2142)
                     /locus_tag="VG274_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWE05145.1"
                     /translation="MILSYRDARTQRFARGERVKAFEPFRKQAEKVLDRLNAATSVRD
                     IAHFPGHRPEKLGGDRKGQWSIRINDQWRICFEWQDGASGPGRVEIVDYH"
     gene            complement(2249..2623)
                     /locus_tag="VG274_00460"
     CDS             complement(2249..2623)
                     /locus_tag="VG274_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05146.1"
                     /translation="MVKALFDTNVLIDFLSGVPQARAEIERFAAAAISIVTWMEVLIG
                     ASDDTQDATRAFLARFELIGLDQQIAEAAIALRRAHRMKLPDAIIWASAREHGRLLVT
                     RNVKDFPAGDPGVRAPYTLRAR"
     gene            complement(2617..2832)
                     /locus_tag="VG274_00465"
     CDS             complement(2617..2832)
                     /locus_tag="VG274_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWE05147.1"
                     /translation="MRTLIDIPDRMAEALTEIGARRKRSRASVVREALSEYLSRHGAG
                     DTNAAFGLWGERGRDGLAWQRKIRAEW"
     gene            2932..>3775
                     /locus_tag="VG274_00470"
     CDS             2932..>3775
                     /locus_tag="VG274_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05148.1"
                     /translation="MSAATEKIAFLRASLAKVDGLEAHARVPLGHPAADACLRGGIQK
                     GALHEIYPTAAGNEAAASGFAVALAVRVAAKKRVLWLRPDFAALEGGEISPLGLLELG
                     FDPTRMLLLRAADAASALRAALDALSCKALGAIVIEIPGAPKILDLAASRRLVLACAQ
                     TGVTTFLLRLLAEPEASAAETRWLIGAARSHPPSGALARLPPPCGEVETCERCSREQI
                     SGGGLCLSMERQRPPPEKREGRFSTSPQGGGKVFVRTRSTSPGQPCASEGGKEEEDWG
                     SPLFE"
BASE COUNT          682 a         1193 c         1176 g          724 t
ORIGIN      
        1 cctccatcgt caccgatcgc attgagacat tcacggagaa agggctgaag ctcgcatcgg
       61 ggacggagct ggaagcggac gtcatcgtca ccgccaccgg acttgtcatg gagcttctgg
      121 gcggcatgga ggtgagcgtc gatgggcagg cgatcgattt ctcgaagacg ctgaattatc
      181 gcggcgcgat gtacagcggc gtgcccaatc tcgcctccgc cttcggctat accaacgcgt
      241 cgtggacgct gaaatgcgat ctcacctgcg aatatgtctg ccggctgctg aaccggatgg
      301 accggcaggg cttgcgccaa tgcacgccgc gcaaccgcga cccgtcgatg ccgaccgagc
      361 cgtggctcga cttctcctcc ggctatgtgc agcgcgcgat ggcgcgcttc cccagacagg
      421 gcacgcgccg accctggaag ctctatcaga actacgccct cgacatcgtg agcctgagat
      481 acgggaagat ggaagacggc gtgctggagt tctccaaccc ggcgccagcg gtttcgaacc
      541 acgggacgga aaggttggcg ggtaaggcct gacccacgcg attgacgtgc gcgatcgtat
      601 tgctgtacaa tttcctgtac atggatggaa gccaatgagc gttgtgacgt acagctacgc
      661 ccgccagaac cttgccaagt tgatggatga cgtcgagcgc gatcgcgccc cggtttacat
      721 ctcgcgtcag cgcaaacgcg gcgccgcggt cctgatgtcg cttgatgatt atcaagcaat
      781 ggaaaccacg ctctatctgc tcagcaaccc caaaaatgcg aagctgttgc gcgaaggaat
      841 tgcggagcta gatgccggca agggcgtcag gcacgacatt gttccagtca ggcggcgcgc
      901 gcgcgcggga tgaatctctt ctggtcgtca ggcgcctggg atgactatgt cgaattccag
      961 gaccgcgacc cggccatggc gcgaaagatc aatgatctga ttgccgacgt caggcgaagt
     1021 ccattccaag gtctgggcaa gccggagcct ttgcgtgggc cgctggcagg ctggtggtcg
     1081 cgccgcatca atcaggagca ccgtttggtc tatcgcgtcg tgggcacgcg cggacgcaat
     1141 cagcgcgtcg aaatcctgca gtgtcgattt cattatgatc gtcggtagga tcgcttgtga
     1201 ttgctgatcc agtcacgcgg cagagcggac ttcggctctg accttggcgc cgagctcggc
     1261 ctcgaccttg tgaagatcat aagcttgctg gatggccagc caaatccgcg caccggtgcc
     1321 aggcgcggtg ccgatccgca gcgccgttcc ggcgtgacgg cgcgcttgcc ggccacgatt
     1381 tcctggatgc gttgcggcgg cacgcggagg cggagcgcga acgccgctgc gctgacgccg
     1441 cgtgcctcaa gctgttcctt caggatcgtg ccgggatgca cggcatgaac ccttttgccc
     1501 tcagccattt catacctgaa tttcgcattt tggcggcggc gagcgagata ggaagccggc
     1561 ctcaagcagc cttgcgccgc ggcaggcgtc tgatctccac tcctttctcc ttctccgcaa
     1621 gacgcaattc atactgcttt tgcagattga gccagaattc cgcgctcatg ccgaaccaat
     1681 ggccaaaacg caaggcggtg tcagccgtta tcgcccgctg ccctctcaga acgccggtga
     1741 tacggtttgt cggaacatcg atctgccgcg cgaactcggc cgccgagagt cccagttcgt
     1801 caagcgcctc cgcaagatgc tcgcccgggt gaatgggagt ccgtgccatg tgtcgcctca
     1861 atgataatcc acgatttcaa cgcgaccggg acctgatgcg ccgtcctgcc actcgaagca
     1921 aatgcgccac tgatcattga ttcggatgct ccattgcccc ttccggtcgc cgccgagctt
     1981 ctccggccta tgccccggaa aatgagcaat gtccctaacg ctcgtcgccg cattcagacg
     2041 gtccagcacc ttctcggcct gttttcgaaa cggctcaaat gccttgaccc tttcgccacg
     2101 tgcgaaccgt tgcgtccgcg cgtcgcggta gctcaggatc atgtgctgga aggctacgca
     2161 tcgttatgct gtacgtcaag cgtaatcaca cccatttgcc gcccagcggt attcgggggc
     2221 cggacactaa aatccaacaa gcccaccgtt accgggcgcg cagcgtatag ggggcgcgga
     2281 cgccggggtc gccggcaggg aagtccttga cgttgcgggt caccaaaaga cggccgtgct
     2341 cgcgggccga ggcccagatg atcgcgtcgg gcagcttcat gcggtgcgcg cggcggagcg
     2401 cgatcgcagc ttccgctatt tgctgatcaa gaccgatgag ttcgaagcgg gcgagaaaag
     2461 cgcgcgttgc gtcctgcgtg tcgtcggaag ccccgatcaa gacctccatc caggttacga
     2521 tgctgattgc ggcggcggcg aagcgttcga tttcggcgcg agcctgcggc acgccgctca
     2581 gaaaatcgat cagcacgttg gtgtcgaaga gcgccttcac cattcggcgc ggatcttgcg
     2641 ctgccaggca agcccgtcgc gtccgcgctc gccccacaga ccgaaggcgg cgttggtatc
     2701 gcctgcgccg tgccgcgaca gatattcgct caaggcctcc cgcaccacgc tcgcgcgcga
     2761 tctcttccgt ctcgcgccga tttcggtcaa cgcctcggcc atccgatccg ggatgtcgat
     2821 caatgtgcgc atcgctgata tatatcatat atcgatatca gatatcaatg tcctctcggc
     2881 taaatcgcgt tgggtttgac tttgttcatg ctttgttcta gttgcacggt catgagcgcc
     2941 gcaacggaga aaatcgcatt cctgcgcgcg tcccttgcga aggtggacgg gctggaagcg
     3001 catgcgcgcg tgccgctggg ccacccggca gccgatgcct gcctcagagg cggcatccag
     3061 aagggcgccc tgcacgagat ttatccgact gcggccggga atgaggccgc ggcgagcggc
     3121 tttgcggtgg cgcttgccgt ccgcgtggcc gcgaagaagc gcgtgctgtg gctcaggccc
     3181 gatttcgccg cgctcgaagg cggcgagatt tcgccccttg gcctgctcga gctggggttc
     3241 gaccccaccc ggatgcttct gttgcgcgcg gccgatgcgg cctcggccct gcgcgcggca
     3301 ctggacgcgc tctcctgcaa ggcgctcggc gccatcgtaa tcgagatccc gggggcgccg
     3361 aaaatcctcg atctcgccgc gagccggcgg ctcgtgctcg cctgcgcaca gaccggcgtc
     3421 accacttttc tgctgcgcct gctggccgag cccgaagcca gcgccgccga aacgcgctgg
     3481 ctcatcggtg ccgcgcgctc gcatcccccc tccggcgcgc tggcgcgttt acctccccct
     3541 tgtggggagg tcgaaacttg cgagcgctgt tcgcgcgagc aaatttcggg tgggggtctc
     3601 tgcctctcca tggagaggca gagaccccca cccgaaaaac gcgaagggcg tttttcgacc
     3661 tccccgcaag ggggaggtaa agtgtttgtt cgtacacgga gcacttcccc cggacaacca
     3721 tgcgcaagcg agggaggaaa ggaggaggag gattggggca gccccctgtt cgaaa
//
LOCUS       DASZMC010000025         6379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3312815,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000025 DASZMC010000000
VERSION     DASZMC010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3312815"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..916)
                     /locus_tag="VG274_00475"
     CDS             complement(47..916)
                     /locus_tag="VG274_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWE05149.1"
                     /translation="MILRSMLFVPADSERKLEKAKSSPADALILDLEDSVAGENRPRA
                     RGLMREFVAHSPARSWVRINSVASPDYRDDLEAVALARPAGIIVPKPDSPETLRALDA
                     DLLALERANGLSERGILVIPVASETPAAVLNLREYRIPPPRLRALTWGAEDLSAALGA
                     TTNRDEHHEFAFTYRLVRSLCLLAAKAAGVEAIETLHADFRDFAGLARVARTANREGF
                     AGMLAIHPDQVEVINAAFTPSLDDVEHARRVVAAFASGVGVASLDGKMLDRPHLKQAE
                     HVLALDNVVRSGR"
     gene            complement(1086..1538)
                     /locus_tag="VG274_00480"
     CDS             complement(1086..1538)
                     /locus_tag="VG274_00480"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWE05150.1"
                     /translation="MLGLYFEEFSVGQRFAHPIRRTVTEADNVFFTAITHNPAQLHLD
                     EEYGKTTEFGTRIVNSLFTLGLMIGISVGETTLGTTVANLGMDEVRFPKPVFHGDTLH
                     VESEVLEMRESRSRLQNGIVVFAHRAYNQRNELVAACKRSALMLRKPK"
     gene            complement(1726..2892)
                     /locus_tag="VG274_00485"
     CDS             complement(1726..2892)
                     /locus_tag="VG274_00485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWE05151.1"
                     /translation="MDISLSDEHRAIDESVSRICTRFSDAYWTECDQTPRFPEEFHRA
                     MAEAGWLGITMPVELGGAGLGVTEAAIMMHAVTRGGGGFSAASTIHVNLFGPHAVVVH
                     GTEEQKARWLPPLIRGEQKVCFGVTEPNAGLDTTNIETFARRTNEGYVIHGRKMWTTT
                     AQEADKILILARTRPRAECNRPTEGMTLFYTDLDRSKVEVRRIEKMGRSAVDSNAVFI
                     DDLSVPDNDRVGEEGKGFRYLLDSLNPERILIGIEAVGLGRDALRRAGDYARERRVFG
                     RAIGQNQGIQHPLAENWAMLESAYWMCLRAGQLYDTKKPCGAEANAAKFLGARAAFDA
                     ATRAVLTHGGMGYAREYQVERLFRESILTRIVPVTEQLVLSFIAENVLDLPKSY"
     gene            3209..4993
                     /locus_tag="VG274_00490"
     CDS             3209..4993
                     /locus_tag="VG274_00490"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWE05152.1"
                     /translation="MKPTDVSNPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRYTDAY
                     MINWESNVFPGVLGRTCDRPCEPACRRLRVEKEPVAICRLKRVAADNKDDISERLPRP
                     AHRKNGKRIALVGAGPASLTVARDLLPLGYSCVLYDQDKMAGGMMRTQIPKFRLPERV
                     LDEECNYILGLEPELRFGEKVGSLKALLAENYDAIFVGCGAPRGRELDIPGRKEAAAN
                     VHIGIDWLSSVSFGHVEKIGKRVIVLGGGNTAMDCCRTSRRLGGEDVRVVVRSGFEEM
                     KASPWEKDDAMMEGIPIHNFLVPRAFLHANGKLTGVRFEKVKAERDEKGRRALVATGE
                     PDVEMECDDVLVAVGQENAFPWIERDIGIEFDRWDMPKIDPVTMQSTNPNIFFGGDSA
                     FGPKNIIWAVAHGHEAAISIDLLCNGDDVNVRPPPLVNLLSQKMGIHEWSYDNDVSLD
                     LRYKVPHAPQDVTLRNVKVEVELGFDPKLGFLEAQRCLNCDIETVFETKLCIECDACV
                     DICPMDCITFTPNADESDLRLHLMAPAPNRAQDLYISDLLKTGRIMAKDEDVCLHCGL
                     CAERCPTGAWDMKKFYLEPAQAESCRNR"
     gene            4978..>6379
                     /locus_tag="VG274_00495"
     CDS             4978..>6379
                     /locus_tag="VG274_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWE05153.1"
                     /translation="MPQPIAATNDFVVKFANVNGSGSASANELFAKCVLRKGVPVATR
                     NIFPSNIQGLPTWYEVRVSAEGWLGRRGGVDLMVAMNPQTWDKDVASIDAGGYLLYDS
                     TKPLPASKFREDLNVIGIPLTEICNRTYTDARQRQLFKNILYVGGLASLLGIEPAIVE
                     GAIAAQFKGKDKLIAPNVQAFRLGYDYAELHLAGACALKVASADGVGDRIFIAGNDAA
                     ALGCVYGGATIAAWYPITPSTSLAEAFSAHCRKLRIDPETKQSKFAIVQAEDEIAAIG
                     MVIGAGWNGARAFTATSGPGISLMQEFFGLAYFAEIPAVIFDIQRGSPSTGMPTRTQQ
                     SDVLLCAYASHGDTKHVLIFPKDPGEAFEFAALSFDLAERLQTPVFVMSDLDIGMNTH
                     LCKPFTWDNSRAYDRGKVMTAEDLESGKDFGRYKDVDGDGIPWRTLPGTHPTKGSYFT
                     RGTTRDAYARYSERGPD"
BASE COUNT         1208 a         2038 c         1991 g         1142 t
ORIGIN      
        1 cttcgccatt ctgatcgcgg tgctttacgg ctggatgatg cgccgttcac cggcctgaac
       61 gcacgacgtt gtcgagcgcc agcacatgct ccgcctgttt cagatgaggc cggtcgagca
      121 tcttgccgtc gagcgaagcg acgcccaccc cgctggcgaa tgccgcaacc acccggcgcg
      181 catgctcgac atcgtcgagt gacggcgtaa aggccgcgtt gatcacttcg acctgatcgg
      241 gatggatggc gagcattccg gcgaacccct cgcggttcgc ggtgcgcgcg acgcgcgcga
      301 gtcctgcgaa atccctgaaa tcggcgtgga gggtctcgat cgcttcgacg ccggccgctt
      361 tcgccgcgag aaggcagagc gagcgcacga ggcgataggt gaaggcgaac tcgtgatgtt
      421 cgtcccggtt ggtcgtagct ccaagcgctg ccgacagatc ttcggcgccc caggtcagcg
      481 cccgcaggcg cggcggcgga atccggtatt cccgaagatt caaaacggcg gcgggcgttt
      541 cgctcgcaac cggaatgacg aggatgccac gctcggacag tccgttcgca cgctccagcg
      601 ccaggagatc tgcgtcgagc gcgcgcaaag tttccggtga atcgggcttg ggcacaatga
      661 tccctgctgg ccgcgcgagc gcgaccgctt cgagatcatc gcggtagtcc gggctcgcga
      721 cggaattgat gcgcacccag ctccgcgccg gcgagtgagc aacaaactcg cgcatgagac
      781 cgcgcgcgcg gggtctgttc tcgccggcca cggaatcttc gagatcgagg ataagcgcat
      841 cggccggcga agacttcgcc ttctccagct ttcgttcgct gtctgccggc acgaacagca
      901 tcgagcggag aatcatgcgt acacccgcgc attcctttcc tcccccgtcg tgggggacgt
      961 ggtttgcgta gcgtagcgaa gcaacccgga ggggggaatc cgcgcgcggc gcgcgcgcca
     1021 ctccctcctc cgcctcgctg cgctcggcac ctcccccacg atgggggagg aaaggattac
     1081 gttcgtcatt ttggcttccg cagcatgagc gccgaacgtt tgcaggccgc gactagctcg
     1141 tttcgctgat tatatgcccg atgagcgaag acgacgatgc cgttttgcag gcgcgaccgg
     1201 ctctcgcgca tttccagcac ttcgctctcc acatgcaacg tgtcgccatg gaacacaggc
     1261 ttcggaaatc gcacttcgtc catgccgaga ttggcgaccg tcgtaccgag cgtcgtctcg
     1321 cccacggaaa tgccgatcat caggccgagc gtgaacagcg aattgacgat gcgcgtgcca
     1381 aactccgtcg tcttgccata ttcctcgtcg agatggagct gtgcagggtt gtgcgtgatc
     1441 gccgtgaaga aaacattgtc ggcctccgtc accgtgcgcc ggatcggatg ggcgaatctc
     1501 tgaccgacgg aaaattcttc gaaataaaga cctagcatgt cgcgagcgcc tcgcctggtg
     1561 gttcgctagt aaatgctaat gtttaccaca aagcacacgc cgtcaaaaaa ccgccaaggc
     1621 gccaaggatg aatcgcgcgc gaatgcgcgt aaaaatctct tggcgtcttg gcggtcaaac
     1681 tttcattcag cccgcgccgc gggatccata gctctcatca gagcctcaat acgatttcgg
     1741 caggtcgagc acattttcgg cgatgaagga gaggacgagc tgctccgtca cgggaacgat
     1801 gcgcgtcagg atgctttcgc ggaagagccg ctcgacctgg tattcgcgcg cgtagcccat
     1861 gccgccgtgg gtgagcaccg cgcgggtcgc cgcgtcgaag gcggcgcgcg cgccgaggaa
     1921 tttcgcggca ttggcttcgg cgccacaggg cttcttcgta tcatagagct ggccggcgcg
     1981 caggcacatc cagtaggcgc tctcaagcat cgcccagttc tccgcgagcg gatgctggat
     2041 gccctggttc tggccgatcg cgcgcccgaa tacgcgccgc tcgcgcgcat agtctcccgc
     2101 gcggcgcagt gcatcgcgac cgagaccgac ggcctcgatg ccgatcagga tgcgttcggg
     2161 attgagactg tcgaggagat agcggaatcc cttgccctcc tcgccgacgc ggtcgttgtc
     2221 gggcacagag agatcgtcga tgaagacggc atttgagtcg acggcgctgc gccccatctt
     2281 ctcgatgcgg cggacctcga ccttcgaacg gtcgagatcg gtgtagaaga gagtcatgcc
     2341 ttcggtcgga cgattgcatt cggcgcgcgg ccgcgtgcgc gcgagaatca gaatcttgtc
     2401 ggcctcctgc gccgtcgtcg tccacatctt ccgcccgtgg atgacataac cttcgttggt
     2461 ccgccgcgcg aacgtctcga tgttggtggt gtcgaggccc gcattgggct cggtcacgcc
     2521 gaagcacact ttctgctcgc ccctgatgag tggcggcaac cagcgcgcct tctgctcctc
     2581 ggtgccgtgc acgacgactg catgcgggcc gaagagattg acgtggatgg tcgatgccgc
     2641 cgaaaaaccg ccgccgccgc gcgttaccgc atgcatcatg atggccgctt cggtgacgcc
     2701 gagcccggcc ccgcccaatt ccaccggcat ggtgatgccg agccagcccg cctccgccat
     2761 cgcgcgatgg aactcctcgg gaaagcgggg cgtctggtcg cattcggtcc aataggcgtc
     2821 gctaaagcgg gtgcaaatac gggaaacgct ctcgtcaatg gcgcggtgtt cgtcggaaag
     2881 agaaatgtcc aaggcagggc tccggtggtg gcgcaggcat tatcgcaagc gcgaccgtcc
     2941 gatccagagc ggttcgacgg aagattttgg ggcaattgcg cgcaagaggc cgtttttcgg
     3001 gccctgcacc cctttagcaa ttgcacgcta agctttgagg gctaatattg cgggattcag
     3061 gggcagcgac cggggcggca gggttgaaat cccgcccgcg aaagaatgcg gccgtcgaag
     3121 ggcggatgcg cgatggagat cgttcacaga gatgagttca gttccggcta aagagcctgg
     3181 cacgctcgag ccagcccgcc cttccggcat gaagccgacc gatgtcagca atccggatta
     3241 cttccacaag gtggtcgact gccaatgggc gtgtccggcg cacacgcccg tgcccgaata
     3301 tatccggctg atcgccgccg gacgctatac cgacgcctac atgatcaact gggaatcgaa
     3361 cgtctttccg ggcgtgctcg gccgcacctg cgaccgcccc tgcgagccgg cctgccggcg
     3421 cttgcgtgtc gagaaggaac ccgtggcgat ctgccggctg aagcgcgtgg ccgcggacaa
     3481 caaggacgac atcagcgagc ggctgcccag gcccgcgcac cggaaaaacg gcaagcgcat
     3541 cgcgcttgtc ggcgcgggcc ctgcctccct gacggttgcg cgcgatcttc tgccgctcgg
     3601 ctatagctgc gttctctacg accaggacaa gatggccggc gggatgatgc gcacgcagat
     3661 cccgaaattc cgcctgcccg aacgcgtgct cgacgaagag tgcaactaca ttctcggcct
     3721 cgagccagaa ttgcggttcg gcgagaaagt gggaagtctc aaagcgctgc tcgcggagaa
     3781 ttacgacgca atcttcgtcg gctgcggggc gccacgagga cgcgaactcg acatccccgg
     3841 acgcaaggag gccgcggcga acgtccacat cggcatcgac tggctgtcga gcgtctcctt
     3901 cggccatgtc gagaaaatcg gcaaacgcgt gatcgtgctg ggcggcggca acaccgcgat
     3961 ggattgctgc cgcacctcgc ggcgtctcgg cggcgaggac gttcgcgtcg tcgtgcgctc
     4021 gggcttcgag gagatgaagg cctcgccctg ggaaaaggac gacgcgatga tggaaggcat
     4081 tcccatccac aatttccttg tccccagggc gttcctccat gcgaacggca agctgaccgg
     4141 cgtgcgcttc gagaaggtga aggccgagcg cgacgaaaag ggccggcgcg cgctcgtggc
     4201 cacgggcgag ccggacgtcg agatggaatg cgacgacgtg ctggttgcgg tcggacagga
     4261 gaatgccttc ccgtggatcg agcgtgacat cggcatcgaa ttcgaccgtt gggacatgcc
     4321 gaaaatcgac ccggtcacca tgcagtcgac caatccgaac atcttcttcg gcggcgactc
     4381 ggcgttcggg ccgaagaaca tcatctgggc cgtggcccat ggccacgagg cggcaatctc
     4441 catcgatctg ctctgcaacg gcgacgatgt gaatgtccgc cccccgccgc tcgtcaatct
     4501 cttgagccag aagatgggca ttcacgaatg gagctacgac aacgatgtct ccctcgacct
     4561 gcgctacaag gttccgcacg cgccgcagga cgtgaccttg cgcaacgtca aggttgaggt
     4621 ggaacttggt ttcgatccga agctcggctt cctcgaagcg caacgctgcc tcaattgcga
     4681 catcgaaacg gttttcgaga ccaagctctg catcgaatgc gacgcctgcg tcgacatctg
     4741 cccgatggac tgcatcacct tcacgccgaa tgccgatgaa agcgatctgc gcctgcactt
     4801 gatggcgccc gcgccgaaca gggcgcagga cctctatatt tccgacctgc tcaagactgg
     4861 ccgcatcatg gcgaaggatg aggacgtctg cctgcattgc gggctctgcg ccgagcgttg
     4921 cccgaccggc gcatgggaca tgaagaaatt ctatctggaa cccgcgcagg ccgagtcatg
     4981 ccgcaaccga tagcagcgac caacgatttc gtcgtcaaat tcgccaatgt gaatggctcg
     5041 ggctcggcga gcgcgaacga gctgttcgcg aaatgcgttc tcagaaaggg cgtgcccgtc
     5101 gccacgcgca acattttccc gtccaatatc cagggattgc cgacctggta cgaagtccgc
     5161 gtctcggccg agggctggct tggccgccgc ggcggcgtcg acctcatggt cgcgatgaat
     5221 ccgcagacct gggacaagga tgtggcgtcc atcgatgcag gcggctacct cctctacgat
     5281 tccaccaagc ctctcccggc atccaaattc cgcgaagact tgaacgtcat cggcataccg
     5341 ctcaccgaaa tctgcaaccg cacctatacg gatgcgcgcc agcgccaatt gttcaagaac
     5401 atcctttatg tcggcgggct tgcgtcgctg ctcggcatcg agcccgcgat cgtcgagggc
     5461 gcaatcgcgg cgcagttcaa gggcaaggac aagctgattg ctcccaacgt ccaggcgttc
     5521 cggctgggtt acgattacgc cgagctgcat ctcgccggcg cctgcgcttt gaaagtggca
     5581 agcgcagacg gggtgggcga ccgcatcttt atcgccggca atgacgcggc agcactgggt
     5641 tgcgtctatg gcggcgcgac catcgcggcc tggtatccga tcacaccatc gacctcgctc
     5701 gcggaagcct tcagcgcgca ttgccgcaag ctccgcatcg atcccgaaac gaagcagagc
     5761 aaatttgcca tcgtccaggc ggaagacgag atcgccgcga tcggcatggt gatcggcgcc
     5821 ggctggaacg gcgcgcgcgc cttcactgcg acgtcgggcc ccggcatctc gctcatgcag
     5881 gaattcttcg gcctcgccta tttcgccgag attcccgcgg tcattttcga catccagcgc
     5941 ggcagcccgt cgaccggcat gccgacccgc acgcaacaat ccgacgtgct gctgtgcgcc
     6001 tatgcgagcc atggcgacac caagcacgtg ctgatattcc caaaggatcc cggcgaagca
     6061 ttcgaattcg ccgccctctc cttcgacctg gcggaacgcc tgcagacgcc cgtcttcgtg
     6121 atgtccgatc tcgacatcgg catgaacacg catctgtgca agccgttcac ctgggacaac
     6181 agccgcgcct acgaccgcgg caaggtgatg acggcggagg acctcgagtc cggcaaggat
     6241 ttcggccgct acaaggatgt cgacggcgac ggcattccgt ggcgcaccct gcccggcacc
     6301 cacccgacca agggcagcta cttcacgcgc ggcaccacgc gcgatgccta tgcgcgctac
     6361 tccgagcgcg ggccggact
//
LOCUS       DASZMC010000026         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3857990,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000026 DASZMC010000000
VERSION     DASZMC010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3857990"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..523
                     /locus_tag="VG274_00500"
     CDS             35..523
                     /locus_tag="VG274_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05154.1"
                     /translation="MGDVVSLVERAAETVDKEKAEALARKMKKGEFDMNDLAEQLGQM
                     KKLGGMQGVLSMLPGVGKVKSQLDAAGLDDKILTRQEAIISSMTKQERTNPDVINGSR
                     RKRIAAGSGVEVSEVNKLLKMHRQMSDVMKKVGKGGRAMQGLAGMFGGGGGMPPGMMP
                     KR"
     gene            550..900
                     /gene="rpsP"
                     /locus_tag="VG274_00505"
     CDS             550..900
                     /gene="rpsP"
                     /locus_tag="VG274_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWE05155.1"
                     /translation="MALKIRLARAGTKKRPHYNIVIADSRSPRDGRFIEKIGFYSPLL
                     PREHADRLKLDTEKAKGWIAKGATASDRVHRFLSDAGVVKARVHNNPKKALPKKKAQE
                     RAAAAAKAAEAPAA"
     gene            907..1431
                     /gene="rimM"
                     /locus_tag="VG274_00510"
     CDS             907..1431
                     /gene="rimM"
                     /locus_tag="VG274_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWE05156.1"
                     /translation="MSRDVLIAAVIGAHGLKGEVKVKTFTATPDALARYRTLHAKDGR
                     LFTIAQLRPGKADEAVATLAEVVDRSTAESLRGLELFVKRDALPAPDEDEFYHADLIG
                     LTAMDEADRVIGTIKAIHNYGAGDVIEIARGDGDSVMLPFVKDFVPVIDLKAGRVVIA
                     EPAEVETGERGNVE"
     gene            1428..1892
                     /locus_tag="VG274_00515"
     CDS             1428..1892
                     /locus_tag="VG274_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE05157.1"
                     /translation="MSDLIFRTATQDDLHAVVTLLSDDELAENREKAIGGDGVGEEYT
                     KAFAAMEHEHYNHILLAEAEGKIVGCLHLTFVPGLSRRGAKRAIIESVRVVTERRGQN
                     VGTAIMKEAIRLARECGCSLVQLTSDTRRARAHLFYRRLGFTQSHFGFKKEL"
     gene            1889..2584
                     /gene="trmD"
                     /locus_tag="VG274_00520"
     CDS             1889..2584
                     /gene="trmD"
                     /locus_tag="VG274_00520"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWE05158.1"
                     /translation="MTWAATVLTLFPEMFPGPLDVSLLGKARQRTLWSLDVRDIREHG
                     LGKHRTVDDTPAGGGPGMVMRTDVACAAIDAVERAGCPLIYLSPRGTPLTQKRVMALS
                     EGPGAILLCGRFEGLDQRAIEAREIEEISLGDFVLAGGEIAAMALIEAAVRLIPGVLG
                     AQDSPTDESFSHGLLEYPQYTRPQSFEGRDIPEVLLGGNHAEIARWRREAAENLTKSR
                     RPDLWPPYEKSKR"
     gene            2635..>2832
                     /locus_tag="VG274_00525"
     CDS             2635..>2832
                     /locus_tag="VG274_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWE05159.1"
                     /translation="MNLLKQLEAEQMKTLRTTAHPAFRPGDTLKVNVKVVEQTYDEKA
                     KQMKTRERIQAFEGVCIARQGE"
BASE COUNT          583 a          857 c          928 g          464 t
ORIGIN      
        1 ccatcccgac cgcgtcgcgg gccgcatcct cgacatgggc gatgtcgtgt cgctggtcga
       61 acgcgccgcc gagacggtcg ataaggaaaa ggccgaagcg ctcgcccgga aaatgaagaa
      121 gggcgagttc gacatgaacg atctcgccga acagctgggc cagatgaaga agctcggcgg
      181 tatgcagggc gtgctgtcga tgctgcccgg cgtcggcaag gtgaagagcc agctcgacgc
      241 cgcgggcctc gacgacaaga tcctcacgcg tcaggaggcg atcatctcgt ccatgaccaa
      301 acaggaacgc accaacccgg acgtgatcaa cggctcgcgc cgcaagcgca tcgcggccgg
      361 ttccggcgtc gaggtcagcg aagtgaacaa gctcctgaag atgcaccgcc agatgtccga
      421 cgtgatgaag aaggtcggca agggcggccg cgcgatgcag gggcttgcag gaatgttcgg
      481 cggcggcggt ggaatgcccc ccggaatgat gcccaaacgc taattggttt tgagagaagg
      541 aagaaagaca tggctttgaa gatcagactt gcgcgcgccg gcacgaagaa gcgtccgcac
      601 tacaacatcg tgatcgcgga ttcgcgcagc ccgcgcgacg gccgcttcat cgagaagatc
      661 ggcttctaca gcccgctgct gccgcgcgag catgccgacc gtctcaagct cgacaccgag
      721 aaagcgaagg gctggatcgc gaaaggcgcg accgcgtcgg accgcgtgca ccgcttcctg
      781 tccgatgccg gcgtcgtgaa ggcgcgcgtg cacaacaacc cgaagaaggc gctgccgaag
      841 aagaaggcgc aggaacgcgc cgctgccgcc gccaaggccg cggaagcacc tgcagcgtaa
      901 tctgtcgtga gccgcgacgt cctgatcgcc gccgtgatcg gcgcgcatgg gctcaagggc
      961 gaggtgaagg tgaaaacctt caccgccacg cccgacgccc ttgcgcgcta tcgcacgctg
     1021 catgcgaagg acggacgcct gttcacgatt gcgcagctgc gtcccggcaa ggccgacgaa
     1081 gcagttgcga cgttggccga agtcgtcgac cgcagcacgg cggagtcgct gcgcggtctg
     1141 gaattgttcg taaagcgcga tgcgttgccc gcgccggacg aggatgaatt ctatcacgcc
     1201 gatctaattg ggctcacggc aatggatgag gccgaccgcg tcattggcac gatcaaggca
     1261 atccacaatt acggcgcggg cgacgtgatc gagatcgcgc gcggtgatgg cgatagcgtg
     1321 atgctgccct tcgtaaagga cttcgtgccg gtgatcgatc tcaaggcggg ccgcgtcgtc
     1381 atcgcggagc ctgccgaagt cgaaacagga gaaagaggaa acgtcgaatg agcgatctga
     1441 tctttcgcac agcaacgcaa gacgatctcc acgccgtcgt gacgctgttg tccgacgacg
     1501 aacttgccga gaaccgcgag aaagccatcg gtggcgatgg cgtcggtgaa gagtatacga
     1561 aagccttcgc cgccatggag catgagcact acaatcacat cctgcttgca gaagcggagg
     1621 gaaagatcgt cggctgcctg catcttactt ttgtgcccgg gctttcgcgt cgcggcgcca
     1681 agcgtgcgat catcgagtcc gtgcgtgttg tcacggaacg gcgcggacag aacgttggca
     1741 cagcgatcat gaaggaagcg atccggcttg cgcgggaatg cggttgctcg ctggtgcaac
     1801 tcacctccga cacgcgccgc gcgcgcgcgc accttttcta tcgccgcctc ggcttcaccc
     1861 agagccactt tggattcaag aaagagctgt gacctgggct gccaccgtcc tgaccctgtt
     1921 cccggagatg tttccggggc cactggacgt gtcgttgctt ggtaaagcgc ggcagaggac
     1981 actctggtcg ctcgatgtac gtgacatccg tgagcacggg ctcggcaagc atcgcacggt
     2041 tgatgacacg ccggccggtg gcggccccgg gatggtaatg cgtaccgatg tggcgtgcgc
     2101 agccattgac gctgtggagc gggccgggtg tccgctgatc tatctctcac cccgtggaac
     2161 gcctctgacg cagaagcgcg tgatggcctt gagcgaaggt cccggtgcga tcctgctctg
     2221 cggccggttc gaggggctgg accagcgcgc catcgaggcg cgggagatcg aagagatcag
     2281 cctcggcgac ttcgtgctgg cgggtggtga gatcgcggcc atggccctga ttgaggccgc
     2341 ggtgcggctg atcccaggtg tcctaggtgc tcaggactcg cctacagacg agagttttag
     2401 ccacggtttg ctggaatatc cgcagtatac ccgcccccag agcttcgagg gccgggatat
     2461 ccccgaggtg cttctgggag gcaaccatgc cgaaatcgct cggtggcggc gggaagccgc
     2521 agaaaacctc accaaatcgc ggcgcccgga cctctggccc ccctatgaaa agtccaaacg
     2581 gtaggctata cgccccggct tcctgtgatc ttttcgcctg acggagactt cccaatgaac
     2641 ctgctcaaac agctcgaagc cgagcagatg aagaccctcc gcacgaccgc tcacccggcg
     2701 ttccgccccg gcgacacgct gaaggtcaac gtcaaggtcg tcgagcagac ttatgacgag
     2761 aaggccaagc agatgaagac gcgcgaacgc atccaggcgt ttgaaggcgt gtgcatcgcc
     2821 cgccagggcg aa
//
LOCUS       DASZMC010000027         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2210358,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000027 DASZMC010000000
VERSION     DASZMC010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210358"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..841
                     /locus_tag="VG274_00530"
     CDS             356..841
                     /locus_tag="VG274_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HWE05160.1"
                     /translation="MKNIINIHATCVLLGRAGVAFGAPRDAGILLLGKSGAGKSDLAL
                     RLIALGAKLVSDDRTDLYVVRGKLFGRAPTTIAGLIEARGVGIVALPVATRARIALVV
                     SLGHPISRLPRRELYKPPHGLSAAVPLLRLAPFEASTPAKIALAVAAFERKLFRESTL
                     S"
     gene            838..1254
                     /locus_tag="VG274_00535"
     CDS             838..1254
                     /locus_tag="VG274_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWE05161.1"
                     /translation="MIGIVLVTHGRLAQEFISAMEHMVGPQTHLRAVCIGPEDDIERR
                     QREIAAAAKSVDMGKGVIVATDMFGGTPCNLALTLLERGKIEVLAGVNLPSLIKLIDV
                     RGKLPLEQAVKEAIDAGRKYMRAGSVDLVGGARACE"
     gene            1247..1528
                     /locus_tag="VG274_00540"
     CDS             1247..1528
                     /locus_tag="VG274_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWE05162.1"
                     /translation="MSDGSRLCAIAPITNKRGLHARASAKIVEASARFQSHITVSKDG
                     QIVDGRSIMGLMMLAASLGSEVEICAEGPDAAEALKAMLALAEAKFGEE"
BASE COUNT          309 a          518 c          524 g          305 t
ORIGIN      
        1 cgccagatcg tggtgaacac cggcgggcgt atctgggccg aaaaccgcga cggaggcggc
       61 gcgcggttca tcgtggaact tccgctggcg aagctggcgt aatgctcaca atgcgagatc
      121 aaatcattga actcaaaaat cctcccccgt ttacggccgc cttcgctaaa tctccggcgt
      181 gccaagagag gctcactcgc cgcggcgaag cctttaagcg gagccggggg gaggtgccga
      241 gcgtagcgag gcggaggggg actttcggcg gcggccccct tcgtctgcct cacgcttcgc
      301 gttcggcagc cacctccccc gtaagcgggg gaggattatc gagcgcaatc tttcgatgaa
      361 gaacatcatc aacattcacg ccacatgtgt gctgcttgga cgcgcaggcg tggccttcgg
      421 cgcgccgcgt gacgcgggga ttctgcttct gggcaaaagc ggcgcgggaa aatccgatct
      481 tgcgctccgg ctgatcgcgt tgggcgccaa gctcgtgagc gacgaccgca ccgatttgta
      541 cgtggtgcgt ggtaaacttt ttgggcgcgc gccgacaacc atcgcgggcc tgatcgaagc
      601 gcgcggcgtg ggcatcgtcg ccctccccgt tgccaccaga gcgcgcatcg cccttgtagt
      661 ttcgctgggg catcccatat ctaggctgcc gcggcgtgaa ctctacaagc ctccgcacgg
      721 cttgtccgcc gccgtgcctc tgctaagact cgcgcccttc gaagcttcga cacctgcgaa
      781 gatcgccctc gcggtcgcgg ccttcgagcg taaactgttt cgcgaaagca ctttgtcatg
      841 atcggcatcg ttctcgtcac ccacggccgc cttgcgcagg aattcatctc ggcgatggaa
      901 cacatggtcg gtccgcagac gcatctgcgc gcggtgtgca tcggacccga ggacgacatc
      961 gaacggcgcc agcgcgagat cgccgccgcc gcgaaatccg tcgatatggg caaaggcgtg
     1021 atcgtcgcga ccgatatgtt cggcggcacg ccatgcaatc tcgcgctcac ccttctcgaa
     1081 cgcggcaaga ttgaagtgct ggcgggcgtc aatctgccga gcctcatcaa gcttatcgac
     1141 gtgcgcggga agttgccgct cgagcaggcg gtgaaagaag ccatcgacgc aggccgcaaa
     1201 tatatgcgcg ccgggtctgt cgatctggtc ggcggggctc gcgcctgtga gtgacggtag
     1261 caggctttgc gccatcgccc cgatcacgaa caaacgcggc cttcatgcgc gcgcatccgc
     1321 gaagatcgtc gaagcgtcgg cgcggtttca gtctcacatc accgtcagca aggacggcca
     1381 gattgtggat ggccgttcca tcatgggcct gatgatgctg gcggcgagtt tgggcagcga
     1441 agtcgaaatc tgtgccgaag ggccagacgc cgccgaagcg ctgaaagcga tgctggcgct
     1501 ggccgaggcg aaattcggcg aagagtaatc agctcccgtc aacttccgcg tcggtcacgc
     1561 cgtcgggaaa gaacactgcg tcgtcctgca gcgcatcttc atcgtcatct tccgaatact
     1621 gctcggaaaa ctgcgccaat gacgcggcat ccggga
//
LOCUS       DASZMC010000028         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3583879,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000028 DASZMC010000000
VERSION     DASZMC010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3583879"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="VG274_00545"
     CDS             complement(<1..1769)
                     /locus_tag="VG274_00545"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HWE05163.1"
                     /translation="MGFEYVLTAPLFAPGAAGDLFLTADHEKPHPAIEQTLNADQLIA
                     ELAENCRDAGLDLLLDLVLGRVASDASVTQSHPEWFADDVITGHAVDPRSALPAPAAA
                     YLRFAEHSADGATAWWIERLLRLANAGASGFCLSQPQLVPCDAWRQIIEIVGRNFPYI
                     RFVAWTPGLRWPEIATLRGVGFDGAFSSAPWWDGRASWFVEEHEVLRGLGAVIASPEA
                     PFGPRLARQLNQTEDPIPLYRHMLHRAAAVGDGLIVPMGFEYAAVDDLDARQTKVAEA
                     LFPSRAGNVDLSSEIRDANALSEELRTLKINGEMRKLTDPGEPVSALIRSDSASVREA
                     TAPIVVFINTDLQHEKPQPIALEPLPPAAGASLVARKRITAEREGHSALAAGEVRILQ
                     ARRSNPIEAARPETTQKKLAAAPRIVIDNVIPSVDGGRIAAKRVIGEPVVIEADVFAD
                     GHGVLGVELLWRSADSRDWLRSPMRTVNNDLWRATIHPERIGRYEFTIEAWINGYGTL
                     ARDIELKRAAGAEFAYELAEAWTALDRASKLVNGSANKVISSALKWLTDASPDERADI
                     LLTPDLREVMYEAEERDFACRRKP"
BASE COUNT          375 a          631 c          644 g          405 t
ORIGIN      
        1 ggcttgcggc ggcaggcaaa gtcgcgctct tccgcttcgt acatgacctc tctgaggtcc
       61 ggggtgagca ggatatcggc cctttcatcg ggcgatgcat cagtcagcca cttcagggcg
      121 ctggaaatga ccttgtttgc gctgccattg acgagtttgc tggcacgatc gagggcggtc
      181 cacgcttcgg caagttcata agcgaactca gcgccggctg cgcgcttaag ttcgatgtca
      241 cgcgcgagcg taccatagcc gttgatccat gcctcgatag tgaactcgta gcgtccgatc
      301 cgttcgggat gaatcgttgc gcgccatagg tcgttgttga cggtccgcat cggcgagcgc
      361 aaccagtctc tggagtctgc gcttcgccaa agcagttcca caccgagcac gccgtggccg
      421 tcggcaaaga catcggcctc gatgacaaca ggctcgccga tcacgcgttt tgccgcaatg
      481 cgcccgccgt cgacacttgg aatgacattg tcgatgacga tgcggggagc cgccgcgagc
      541 tttttttgtg ttgtttccgg tcgcgcggcc tctattggat tgctcctgcg cgcctgcagg
      601 atacggactt cgcccgctgc aagcgcggaa tgtccttcgc gctcggccgt aatccttttg
      661 cgagccacga ggcttgcgcc agccgcgggc ggcagcggtt caagagcgat tggctgtggc
      721 ttctcgtgct ggagatcggt attgatgaac accacgatgg gcgccgtcgc ttcgcgcacg
      781 cttgcgctat cggaacggat aagggcgctg accggctctc ctggatcggt caacttccgc
      841 atttctccgt tgatcttcag agtccgcagt tcctcgctca gcgcgtttgc gtcgcggatt
      901 tcagaggata ggtcgacgtt gccggcccgc gacgggaaaa gcgcttccgc caccttcgtt
      961 tgacgcgcat ccaaatcgtc gactgccgca tattcgaacc ccatgggcac gatgaggccg
     1021 tcgccgacgg cggccgcacg gtgcagcatg tgccggtaga gggggatcgg gtcctcggtc
     1081 tggttcaatt gacgcgccag tcgcggcccg aacggtgctt caggcgatgc aattaccgct
     1141 ccgagcccgc gcaacacttc atgctcttcg acaaaccagc tggcgcggcc gtcccaccat
     1201 ggcgcggacg agaacgcgcc atcgaaacca accccgcgca gcgtcgcgat ctcgggccag
     1261 cgcagtcccg gcgtccatgc gacgaagcga atgtagggaa aattccgtcc aacaatctcg
     1321 atgatttggc gccaagcgtc gcacggtact aactgcggct gcgacaggca aaatcccgac
     1381 gcgccggcgt tcgccaagcg aagcaggcgt tcgatccacc atgccgtcgc gccatcggca
     1441 gagtgttcgg cgaagcgcaa atacgccgca gcaggcgccg gcagagcgct ccgtgggtca
     1501 acagcgtgac cggttatgac gtcgtcagcg aaccattcgg gatgcgactg cgtcacgctc
     1561 gcatcgctcg cgacgcggcc gaggacaagg tcgaggagaa ggtccagtcc ggcatcgcgg
     1621 cagttttccg ccaattcggc aatcagttga tcggcattga gcgtttgttc gatcgccggg
     1681 tgaggctttt catggtcggc ggtaagaaac agatcgccgg ccgcccccgg cgcgaacagt
     1741 ggcgctgtca gcacgtattc gaagcccatg gcacggcatc gctgcaggtg caggggccag
     1801 gatgggcgcg gtccggctag aagcggatgg aagtagtaga ttcggggtgc aaacagatca
     1861 cggccccctt tgaccacgct tgcacgctgg cccatcgcca taaatcagat cgcccccttg
     1921 atccccttga ccgttccagc gggttcatga gttcccttgg tcaaaacggt cgcgtgggaa
     1981 aagttccatg gagagcacgc taggtagccg ttgcgggagg ccgttgctgt gctgtccgag
     2041 cgccattact tgacg
//
LOCUS       DASZMC010000029         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4139223,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000029 DASZMC010000000
VERSION     DASZMC010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4139223"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..2234
                     /gene="dcp"
                     /locus_tag="VG274_00550"
     CDS             84..2234
                     /gene="dcp"
                     /locus_tag="VG274_00550"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: protein motif:HMM:NF007624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HWE05164.1"
                     /translation="MLRFRTLCLVTAAAFMLAAVPLLLQAAPAAPAAPSANPFAEPSP
                     LPLQAPPFDRIKDSDYQPAFEEAMRQQIAEIEAIANNPAAPTFENTIVAMEKSGRMLD
                     RVNLTFSGVVQANTNDTLDHVQTEEAPKLAAHQDAISLNPKLFARVTAVYDARDGLKL
                     DPESLQLLKIYYMQFVHSGANLSDADKVKLREINKRDASLEAAYQQKLVAAAKAGALV
                     VDDKAALAGMSDQEISAAAQAAKARKLEGKFVIPLQNTTQQPALASLSDRNVREKLFD
                     SSWTRAEKGDKNDTRATIEELAKIRADKAKLLGYPNYAAYVLYDQMAKTPDAVLKFME
                     QLAPATRAQAAREAKDIQALIDKQGAHFDLKPWDWEFYSEQVRKAKFDIDEKQIKPYL
                     ELNNVLQNGVFYAANKLYGLTFKERKDIPVYQPDVRVFDVLDKDGSQLGLFYFDYFKR
                     DNKSGGAWMSNFMEQSKLIGTKPVIYNVANFAKPAPGQPALLTFDDVTTMFHEFGHGL
                     HGLFANQIYPSISGTSVARDFVEFPSQFNEHWALYPEVLKHYAVDYRTGKPMPQALID
                     KIKKAAKFDQGYKLGEVVAAAELDMQWHMLSADRPKEDCDAFEPQALKAANVDFPDVP
                     PRYRSSYFLHIWANGYAAGYYAYLWTEMLDDDAFAWFKQHGGLTRENGERFRKMILSR
                     GHSEDYGPMFRAFYGKDPEIGPMLQHRGLAPGGD"
     gene            complement(2428..2946)
                     /locus_tag="VG274_00555"
     CDS             complement(2428..2946)
                     /locus_tag="VG274_00555"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWE05165.1"
                     /translation="MTMFTRSDKEPVPKPAATPPSPAPAPAPAPVEARRAPAPPMHGQ
                     ASSVSVISKALKITGQLESTEDIQIEGEVEGDVRAASVKVGTNARIKGTVYGDEVELS
                     GKVDGKIEARKVVLTSTAHMSGDVIHQDLRIDSGAHVDGHCRPEFGKPDGRAHAIQKP
                     APGATATPLKPN"
     gene            complement(2943..>3201)
                     /locus_tag="VG274_00560"
     CDS             complement(2943..>3201)
                     /locus_tag="VG274_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWE05166.1"
                     /translation="RADRLVIEKGGAVEGDMVARDAHIGGRLNGRVFAVNVTIDASAD
                     VRGRIFHTTVSVASGARIDGRMPWRPVNYFETLETLPETQP"
BASE COUNT          620 a         1017 c          979 g          585 t
ORIGIN      
        1 acctataccc ttcaatctga ctgaatcggg cgactagcct atctcgcctg acgcttgccg
       61 gcgcctatcc aacggggaat ttcatgcttc gttttcgcac gctttgcctc gtcactgccg
      121 ccgcgtttat gctcgctgcc gtgcctcttt tgctccaagc cgcgccggcg gctcctgctg
      181 ccccttcggc gaacccgttc gccgagccaa gcccgttgcc gcttcaggcg ccgccgttcg
      241 accggatcaa ggacagcgac taccagccgg ccttcgaaga ggcgatgagg cagcagatcg
      301 ccgagatcga ggcgatcgcc aacaatcccg ccgcgcccac cttcgagaat acgatagtcg
      361 cgatggagaa atcgggccgc atgctcgacc gggtgaacct caccttctcg ggcgtggtcc
      421 aggccaacac caacgacacg ctcgatcacg tgcagaccga agaggcgccg aagcttgccg
      481 cgcatcagga tgcgatctcg ctgaacccga aactcttcgc gcgcgtgacg gcggtctatg
      541 acgcacgcga cgggttgaag ctcgatccgg aatcgctcca gctcctcaag atctattaca
      601 tgcagttcgt gcattccggc gccaatctgt cggatgccga caaggtgaaa ttgcgcgaga
      661 tcaacaagcg cgacgcctcg ctcgaggcgg cgtatcagca gaagcttgtg gccgcggcga
      721 aggccggcgc gctggtggta gacgacaagg cggcgcttgc agggatgagc gaccaggaaa
      781 tctccgccgc cgcgcaggcc gcgaaagcac gcaagctcga aggcaaattc gtgatcccgc
      841 tgcagaacac cacccagcag ccggcgctcg cctcgctgag cgacaggaac gtgcgcgaga
      901 aattgttcga cagttcctgg acgcgggcgg agaagggcga caagaacgac acgcgcgcca
      961 cgatcgagga gctggcgaaa atccgcgccg acaaggccaa gctgctgggc tatccgaact
     1021 atgccgctta cgtgctctac gaccagatgg cgaagacgcc cgatgcggtg ctgaaattca
     1081 tggagcagct cgctcccgca acgcgggccc aagccgctcg tgaagcaaag gacatccagg
     1141 ccctgatcga caaacagggc gcacatttcg atctcaagcc ctgggactgg gaattctatt
     1201 ccgagcaggt gcgcaaggcg aagttcgaca tcgacgagaa gcagatcaag ccctatctcg
     1261 agctcaataa tgtcctgcag aacggcgtct tctacgccgc gaacaagctt tacggattaa
     1321 cgttcaagga gcgcaaggat attccggtct atcagccgga cgttcgcgtg ttcgatgtgc
     1381 tcgacaagga cggctcgcag ctggggcttt tctatttcga ctatttcaag cgcgacaaca
     1441 aatcgggcgg cgcctggatg tcgaatttta tggagcagtc aaagcttatc ggtaccaagc
     1501 cggtcatcta caatgtcgcg aatttcgcca agccggcgcc aggccagccc gcgctgctga
     1561 cctttgacga tgtaaccacg atgttccacg aattcggcca cgggttgcac ggcctgttcg
     1621 cgaaccagat ctatccgtcg atttcgggca ccagtgtggc gcgcgatttc gtcgagtttc
     1681 cgtcgcagtt caacgaacac tgggcgctct atcccgaggt tctcaagcac tacgcggtcg
     1741 actaccgcac aggcaaaccg atgccgcagg cgctgatcga caagatcaag aaggcggcga
     1801 aattcgacca gggctacaag ctgggcgaag tggtggccgc agccgagctc gacatgcaat
     1861 ggcacatgct ttcagccgac aggccgaagg aagattgcga cgccttcgag ccgcaggcgt
     1921 tgaaggccgc gaacgtggat ttccccgacg tcccgccgcg ctaccgctcg agctacttcc
     1981 tgcacatctg ggctaacggc tacgccgccg gctactacgc ctatctgtgg acggaaatgc
     2041 tcgacgacga tgcattcgcc tggttcaagc agcatggcgg cttgacgcgc gaaaatggcg
     2101 agcggttccg caagatgatc ctttcgcgcg gacacagcga ggattacggg ccgatgttcc
     2161 gcgctttcta cggcaaggat cccgaaatcg gccccatgct ccagcaccgc ggcctcgccc
     2221 ccggcgggga ttgaggcgca cccctcaccc acgactgagc agaattcgcc gcccgtatct
     2281 gcacacctct cccacagggg agaggggaaa tcagagtcct gcatcggcgg tggaaaatca
     2341 gtgcatggcg cccctctccc cttgcgggag agggctcaag aatggggact atcaatcacc
     2401 tcagtgcggg tgaggggccg ctctgcccta gttcggcttt agcggcgtcg cggttgcgcc
     2461 gggggcgggt ttctggatcg cgtgggcgcg gccgtcgggc ttgccgaatt cggggcggca
     2521 atggccatcg acgtgggcgc cggaatcgat ccgcagatcc tggtggatga cgtcgccgct
     2581 catatgcgcg gtgctggtga gcacgacctt gcgcgcctcg atcttgccgt ccacctttcc
     2641 ggacagctcg acctcgtcgc cgtagaccgt tcccttgatc cgcgcattgg tgccgacctt
     2701 gaccgacgca gcgcgcacgt cgccttcgac ttcgccttcg atctgaatgt cctcggtgct
     2761 ctcgagctgg cccgtgattt tgagcgcctt gctgatgacc gacacggacg acgcctgccc
     2821 gtgcatggga ggcgcgggag cccgcctggc ttcgacggga gcgggcgctg gcgcgggcgc
     2881 gggtgacggc ggcgtggcag ccggcttcgg tacaggttct ttgtcgctgc gcgtgaacat
     2941 ggtcatggct gtgtctccgg gagggtttcg agagtttcaa agtaattgac cggccgccag
     3001 ggcatgcgcc catcgatccg tgcaccactc gcgaccgaga cggtggtgtg gaaaattcgt
     3061 cccctgacat ccgccgaggc gtcgatcgtc acgttcacag cgaagacgcg cccgttcagg
     3121 cgcccgccga tatgcgcatc gcgcgccacc atgtcgcctt ccactgcgcc gcccttttcg
     3181 atgaccaggc ggtcggcccg t
//
LOCUS       DASZMC010000030         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_836260,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000030 DASZMC010000000
VERSION     DASZMC010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836260"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VG274_00565"
     CDS             <1..369
                     /locus_tag="VG274_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HWE05167.1"
                     /translation="AWANYRDESFIEQFLSPRLIREFRLFELHDKADDESYRVSAIHD
                     ESGYRRIRSSLARQYDVGLMDPNIQVADADLKGDRKLRLVHLMHRGIPLHAKTRDLVC
                     THLERLWGHEVVLDEVDGGQ"
     gene            487..726
                     /locus_tag="VG274_00570"
     CDS             487..726
                     /locus_tag="VG274_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05168.1"
                     /translation="MQLKHVLLAAALVVAPLAAAMPASAVVVGVHVGVPGPGHYCGWH
                     HRCWGYRWHGGYYNYYWHGRYWRDRWMCGRRWCYR"
     gene            complement(774..2024)
                     /locus_tag="VG274_00575"
     CDS             complement(774..2024)
                     /locus_tag="VG274_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE05169.1"
                     /translation="MPLRHVAAVAAGNALGFYDFLTYAFFAVHIGHTFFPSRDPASSL
                     LLSLATFGAGFFTRPLGGIVIGMLGDRKGRKPAMFVSFSLMGAAIVGLALTPSYRAIG
                     IAAPILAISFRLLQGFALGGEVGPTTAYLIEAAHERNRGLFGSLQYATQDLAILCAGL
                     VGVALANLLDASALDAWGWRIAFLIGTAIVPFALLIRRQLPETLDERPADREAREPLR
                     PHLRVALLGLLLLGSGTIASYVLVYMTTYGVATLHIASNYAFGATVVGGFCGVCFDPV
                     SGWLSDRFGRKRVMMAPWALLFFAVLPVFMLMAHFRSAAALYAATAAMQILVSLGAPA
                     IVIAITEALPPHIRSGALSTIYAVAIATFGGTTQFVITWLIARTGNPLAPAWYLMGAI
                     VVGLMTMLAFEETAPAAALSRRAR"
     gene            2330..3370
                     /locus_tag="VG274_00580"
     CDS             2330..3370
                     /locus_tag="VG274_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05170.1"
                     /translation="MIAKCWLHIGTEKTGSTSIRNYLALNRAQLLSQGYLYPRTPGTT
                     NHYALTAFALDDARIDGTRRTLGIGEPEGARRFRAEFVDSLKKETAASGASTVIFSNE
                     LLSSRLRSPAELARIKALCDGIARETKIVVYLRNQADFLVSRYMNIVWEGGTKEFRFR
                     SPPALADYSRLLDRWAQAFGRENIVARRFEPADFPGGDLLADFAGATGLDHARLRPVP
                     RFNESLDAESLAFLRAMNRRLPPQIAGRIAAFRGRIVRVLQRRRGGSRFIISNAVARQ
                     IEEAFRDSNELVSNAYFESRFHPLFSRPLLAGPPDALPNNDIRPLAAFAIAGFLTAGL
                     AWNFVKSRFVKS"
     gene            3569..3724
                     /locus_tag="VG274_00585"
     CDS             3569..3724
                     /locus_tag="VG274_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05171.1"
                     /translation="MAAHPRLLMILYGATPYCGAADGVTLSVTKIQLLVGARPRHFWA
                     EAHTALY"
BASE COUNT          795 a         1218 c         1215 g          706 t
ORIGIN      
        1 gcgtgggcga attatcgcga cgagagcttc atcgagcagt ttctgagtcc caggctcatc
       61 cgcgagttcc ggctgttcga gctccacgac aaggcggatg acgagtccta cagggtctca
      121 gccatccacg acgaaagcgg ctatcgccgc attcgcagtt cgctggcgcg gcagtacgat
      181 gtgggcctca tggatccgaa cattcaggtc gccgacgcag atctgaaagg ggaccgcaaa
      241 cttcgtctcg tgcatctcat gcaccgggga atcccgctgc acgcaaagac acgggatctc
      301 gtctgcacgc atctggagcg cctttggggg catgaagtcg tcctggacga ggtggacggc
      361 ggccaataag gctgaatttt tagccggcag agtggatagt gtgataaaca tttgctgagt
      421 cgaagccagc tcgctcgcga aggtgtgttc tgcgggttca ggcggcaaaa agatcaggag
      481 tattcgatgc aattgaaaca tgtgcttctg gccgcggcat tggtcgtcgc gcctctcgcc
      541 gccgcaatgc cggcttcggc tgtcgtggtt ggcgtacacg tcggcgttcc ggggcctggc
      601 cattactgcg gctggcatca ccgttgctgg ggctatcgct ggcacggcgg ctactacaat
      661 tactattggc acggccgtta ttggcgcgat cgctggatgt gcggccggcg ctggtgctat
      721 cggtaggatt gcgtgatgcg ggcgagcggg cttcggtgcc tccttcgccc gcgttatcgc
      781 gctcgccgcg acaacgccgc ggcaggagcg gtttcttcga atgcgagcat ggtcatcaat
      841 cccactacga tcgcgcccat cagataccaa gccggggcga gcggattacc cgtccgcgca
      901 atgagccacg taatcacgaa ctgcgtcgtt ccgccgaaag tggcgatggc gacggcatag
      961 atcgttgaca gcgcgccgga ccggatgtgc ggcggcagcg cttccgtgat tgctatgaca
     1021 atcgcaggcg cacccagcga cacgaggatt tgcatcgcgg ctgtggctgc ataaagcgcc
     1081 gcggcggagc ggaaatgcgc catgagcatg aagaccggca gcaccgcaaa gaaaagcagc
     1141 gcccatggcg ccatcatcac ccgctttcgt ccgaaacggt cggacagcca tccgctgacc
     1201 ggatcgaagc agacgccgca gaaccctccg accaccgtcg ccccaaacgc gtagttcgac
     1261 gcgatgtgca gcgtcgcaac gccataggtg gtcatgtaga cgagcacata gctcgcgatc
     1321 gttccgctgc cgagcagcaa cagcccaaga agagcgaccc gcaaatgggg ccgcaagggc
     1381 tcgcgcgcct cgcggtcggc cggccgctcg tcgagcgttt cgggaagctg gcgacggatc
     1441 agcagggcga agggcacgat cgcggtgccg atcagaaatg cgatgcgcca gccccaggcg
     1501 tcgagcgccg atgcatcgag aaggttggca agcgcgacgc cgaccagtcc cgcgcacaat
     1561 atggcaaggt cctgcgtcgc gtattgaagc gagccgaaga ggccgcgatt tctctcatgc
     1621 gcggcttcga tgagataagc cgtggtcgga ccgacttccc cgccaagtgc gaaaccctgt
     1681 agcagccgga acgagatcgc cagaatgggc gccgcgatgc cgatggcgcg gtaggaaggc
     1741 gtaagcgcca gcccgacgat cgccgcgccc atgagtgaaa aggagacgaa catggcgggc
     1801 ttgcgcccct tccgatcgcc cagcatgccg atgacgatcc cgcccagggg gcgcgtgaag
     1861 aaacccgcgc cgaacgtcgc aagcgagaga agaaggctgg acgcgggatc gcgcgagggg
     1921 aagaaagtat gtccgatatg gaccgcaaag aacgcgtagg taagaaaatc gtagaacccg
     1981 agggcattgc ctgccgccac cgccgcaacg tgacgcagcg gcaagccaac ccgggcgggc
     2041 gccggtattt cgtccaacgc cgctacagtc gcgccagccg cccgagttac ctcgcctcga
     2101 cgatccgatg tagcatgttt tcaaaggaaa cttgatcgcc gcctcatttt ccttccccaa
     2161 cgtgggggca gtgccgagcc ccccgatccg cgcgaagcgc ggccggagga taaactccgc
     2221 gaggcggagg ggggagttgt gtgcgattac atgcccctcc ggcccttcgg gccacctccc
     2281 ccacattggg ggaggaaatg tggcctggcg gtgcgatgaa agggcgcgaa tgattgcgaa
     2341 gtgctggctg catatcggca ccgaaaagac cggcagcacc tcgatccgga actatctcgc
     2401 gctgaacagg gctcaactcc tgtcgcaggg ctatctgtat ccacggacgc cgggcacgac
     2461 caatcactat gcgctcaccg ccttcgctct tgacgacgcg aggatcgacg gcacgcggcg
     2521 cacgctcggc atcggcgagc cagaaggcgc gcgccgcttc cgcgccgaat tcgtggactc
     2581 actcaagaag gaaaccgccg ccagcggtgc aagtacggtc atattttcga acgagctttt
     2641 gagctcccgg cttcgctcgc ctgccgaact cgcgcgcatc aaggcattgt gcgacggcat
     2701 agcgcgagag acgaagatcg tcgtttatct ccgaaaccag gccgattttc tggtcagccg
     2761 ctacatgaac atcgtgtggg aaggcggtac gaaggaattt cgctttcgga gtccgcctgc
     2821 gctcgccgac tattcgcggc tgctcgaccg atgggcgcag gcgtttggac gcgagaacat
     2881 tgttgcgcgg cggttcgagc cggcggattt tcccggtggc gatcttctcg ccgatttcgc
     2941 cggcgcgacc ggtctcgatc acgcaaggct tcggccggtt ccccgcttca acgaatcgct
     3001 cgacgccgaa tcgctcgcct tcctgcgcgc gatgaaccgc cgcctgccgc cccaaatcgc
     3061 cggccgcatc gctgcgttcc gtggccgtat cgtgagggtg cttcagcggc gtcgcggcgg
     3121 cagcagattc atcatctcca acgccgttgc caggcagatc gaggaagcat tccgcgattc
     3181 gaatgaactg gtctccaacg cgtatttcga atcgcgcttt caccccctct tttcgcggcc
     3241 gcttctcgcc gggccgcccg acgcgctgcc aaacaacgac atcagacctc ttgctgcatt
     3301 cgcaattgca ggatttctga ccgccggcct cgcctggaac tttgtgaagt cgcggttcgt
     3361 caaatcctga aacgccaact cttgaattgc gggcgaacgg aatcgaaccc tcggtataca
     3421 gcttggaaag cagattttgt ccaggcatgc tcaacgtgtt gcgagaaaaa ctgcaccaat
     3481 gcgcccataa tcggatcaat agcccatgcg cccgccgaaa aacttcaaaa gcaccgactg
     3541 gcgcgtcgcc ggcgggtgaa gcaccacatt ggcggcgcat ccgcgccttt tgatgatcct
     3601 ctacggcgcc acaccctatt gcggcgccgc agatggagtg acgttgagcg taacgaaaat
     3661 acaactgctg gtcggcgccc ggccgcgtca tttttgggcg gaggcgcaca cggcgcttta
     3721 ctaacgggtg gcaagtcttc tgtatgcgaa gaggaatctc atctgtctgc gaggagaaaa
     3781 accaatgact gccaatcgca aactgtttcg gaatgtcctg ctcggctcgg tgttcgcgct
     3841 caccgctttt accgcgagcg cacaggcgca aagctatacc gtgctgcaca acttcgccgg
     3901 accgcccggt gacggctcct atccctataa cgat
//
LOCUS       DASZMC010000031         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2213784,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000031 DASZMC010000000
VERSION     DASZMC010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213784"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..325)
                     /locus_tag="VG274_00590"
     tRNA            complement(233..325)
                     /locus_tag="VG274_00590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Ser,seq:gga)
     gene            complement(405..833)
                     /locus_tag="VG274_00595"
     CDS             complement(405..833)
                     /locus_tag="VG274_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05172.1"
                     /translation="MFRVFPVLLIVVVIYNLVAVAHGLAGNEAMQTFLAREHQTFRMF
                     SGDSWDFSLGDLLLTLGFICLFIEIVKATRTTRRALVNHGLSMLVFVITLVEFIVVKG
                     FSTSVFFFILIMTAFDVVAGYTISVVSAEHDLGVGRAGTD"
     gene            complement(907..1230)
                     /locus_tag="VG274_00600"
     CDS             complement(907..1230)
                     /locus_tag="VG274_00600"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HWE05173.1"
                     /translation="MSAHWITPDRLWLLTGLVGQGLFASRFIIQWFRSEREGRSVIPV
                     AFWYCSLGGGVVTLAYTVHLQSVPLTLGQASGLIVYSRNLYLIFRERSLLREAAQTHP
                     STSSG"
     gene            complement(1231..1959)
                     /locus_tag="VG274_00605"
     CDS             complement(1231..1959)
                     /locus_tag="VG274_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWE05174.1"
                     /translation="MAVALSVVVPVKDEAENVGPLAREIADAVRHDASVEIIFVDDGS
                     EDDTAARLKQLQIDIPSLRVVQHGRNAGQSRALRSGVRAAHGILVVTLDGDGQNDPAD
                     IARVLAPFHAEGDPTRIGMVSGVRVKRKDPLVKRVVSRLANRFRIRMLRDGATDVGCG
                     LKAFRRDAFLALPYFDHMHRYLAALMQREGYEVRFVDVGHRPRLHGRSKYGVWDRLVV
                     GVADILGVLWLQKRFRGAVETKEL"
     gene            2158..3879
                     /locus_tag="VG274_00610"
     CDS             2158..3879
                     /locus_tag="VG274_00610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWE05175.1"
                     /translation="MTEGAAQAEGSAAPWLERAARRPVLALLLLAMFAWLPGLSTLPP
                     LDRDESRFAEASRQMAETGNHIDIRFAGGTRYNKPIGIYWLQAAAATLAGPALRSRIS
                     VYRVPSLLGGILSLILTYECARGFASRKTALMAATLLGSTLLLTAESDIATTDAALLS
                     TIVAAQAVMMRVYLAARDARHGKPGLAAILAGWAALGVGVLLKGPVILAVLAATALAL
                     SLWDRDWRWLRGTRPLPGLVLAAAIVAPWAIAIGLASHGAFYQQSLGHDFAAKILGGE
                     ESHGAPAGYYLLLASFTFWPATVFLIPALGSAIVRRGDPVNRYLLAWSGAAWLMFELV
                     PTKLPHYILPVYPALAILCALWAAGSNRELETRRTSVLHYAAGAQFGLAALALAAASL
                     VLPLRFGGAVNWLLIAGTALASAASWAAIFFVIRQRVDSAAAAAIASAVAFYLLLAFA
                     VAPQLHDIWISPRAAKLVADNRRPDDPPPVVAGYVEPSLVFALGSDTRIEPADGAARA
                     AAAHGGLALVDGQAEPIFLRGLPSFHAAARQLGQISGFDYSNERKERVILYRVTPSPR
                     TPATPEN"
BASE COUNT          698 a         1305 c         1269 g          731 t
ORIGIN      
        1 gataacgggg cacccggatt tcgcgacacc gccaagggct atccgccagg ctactatgcc
       61 gccttcgtgc tggatccgga tggcaacaat atggaagctg tctttcgcgg ccgataagga
      121 ccgctttcct gtctgctcct gctcctgctc ctgctcctgc gccggcgacg atgcagatcc
      181 ccttgcaggc agtcggcgga tcggtcgtag catggcgcag aatcgacgga tttggcggac
      241 agggagggat tcgaaccctc gagacgattc gtcaccgtct acacactttc caggcgtgcg
      301 ccttcaaccg ctcggccacc tgtccgcaac atccgggcag cgcgcccggg aaagggccgg
      361 gactatagcg gctggtcccg gcacggcaag attccggcgg aaattcagtc cgttcccgcc
      421 cggcccacgc ccagatcgtg ctcggccgac acgaccgaga tcgtgtaacc cgccaccacg
      481 tcgaatgccg tcatgatcaa gatgaaaaag aagacggagg tcgagaagcc cttcacgaca
      541 atgaactcga ccagggtgat gacgaaaacg agcatggaga gtccgtgatt gacgagcgcg
      601 cgccgcgtcg ttcgcgtcgc tttcacgatc tcgatgaaca ggcagatgaa accgagtgtg
      661 agcagcagat cgccaagact gaaatcccac gaatcgcccg agaacattcg gaaggtctgg
      721 tgttcgcggg cgagaaacgt ctgcatcgct tcattgccgg cgaggccatg agccaccgcg
      781 acgagattgt agatgacgac gacgatcagc agcactggaa agacacggaa catttgggat
      841 ccccctgttc agactcacgc ttcgaccagc tcacccttcg acaggctcag catgaggaca
      901 aaatcctcac cctgagcttg tcgaagggtg agtttgcgcc gcttcgcgca gcagactgcg
      961 ttcgcgaaag atcaggtaga gattgcggct gtagacgatg aggccgctcg cctggcccag
     1021 tgtgagtgga acgctctgaa ggtgcaccgt ataggcgagc gtgaccactc cgccgcccag
     1081 gctgcaatac cagaacgcga ccgggataac gctgcggccc tcgcgctcgc ttcgaaacca
     1141 ttgaatgatg aagcgggatg cgaagagacc ttggccgaca agcccggtca ggagccagag
     1201 gcgatccggc gtgatccaat gtgccgacat ctacaactcc ttcgtctcga ccgcgccgcg
     1261 aaagcgcttc tgcagccaga gcacgccgag aatgtccgcg acgccaacga ccagcctgtc
     1321 ccacacgcca tatttcgagc ggccgtgcag gcgagggcga tgcccgacat ccacgaagcg
     1381 cacctcgtag ccctcgcgct gcatgagcgc ggcgagatag cgatgcatgt gatcgaaata
     1441 gggaagggcg aggaaggcgt cccgccgaaa cgccttgagt ccgcagccga catcggtggc
     1501 gccgtcgcgc agcatccgga tgcggaatcg attggcgagc cgggagacga ctcgcttcac
     1561 aagcgggtcc ttccgtttca cgcgtacgcc cgacaccatg ccgatccgcg tgggatctcc
     1621 ctcggcgtga aacggcgcca gcaccctggc gatgtcggcg ggatcgttct ggccgtcgcc
     1681 atcgagcgtg acgacaagga ttccatgcgc tgcgcgcacg ccgctgcgca gcgcccggct
     1741 ctgcccggcg ttgcggccat gctggaccac gcgcagcgaa gggatgtcga tttgaagctg
     1801 cttgagccgc gcggccgtgt cgtcttcgga cccgtcatcg acgaagatga tctcgaccga
     1861 agcgtcgtgg cggacagcgt cggcaatctc ccgggcgagc ggtcccacat tctctgcttc
     1921 atccttgacc ggcacgacca ccgataatgc gaccgccatc actgaaggtt ccgttcgctc
     1981 ctgccgtaca atagaatgcg aatcgcgcgg tgcagtgccc gtttctgcag actggacgga
     2041 gcccggcgcc gttgccgggc gcgcgcctta tacaggtgca aagcgcgcgg aaacaggcct
     2101 tggccgcagc gcgcgtccgc ggggccgtag cggccaacag gcgggagtcg gatgctcgtg
     2161 acggaagggg cggcgcaggc ggagggttcg gctgcgccgt ggctggagcg cgcggccagg
     2221 agaccggtgc tggcgctcct gctgcttgcg atgttcgcgt ggctcccggg tctttcgacg
     2281 ctcccgccgc tcgaccgcga cgaaagccgt tttgcggagg cgtcgcggca gatggcagaa
     2341 accggaaacc acatcgatat ccgttttgca ggcggcaccc gctacaacaa gccgatcgga
     2401 atctactggc tgcaggcggc cgccgcgacg ctcgcgggcc cggcattgcg cagccgcatt
     2461 tccgtctatc gcgttccctc gctgctcggc ggcatcctct cgctgatcct cacctatgaa
     2521 tgcgcgcgcg gctttgcttc gcgaaagacc gcgcttatgg ccgcgaccct gcttggatcg
     2581 acattgcttc tgaccgccga gagcgacatc gcgacaacgg atgccgcatt gctttccacc
     2641 atcgttgccg cacaggccgt gatgatgcgg gtctacctcg ccgcgcggga tgcgcgacat
     2701 gggaaaccag gtctcgcggc gattctcgcc ggatgggccg ctctgggcgt cggcgtgctg
     2761 ctgaaaggtc ccgtcatcct cgccgtgctg gccgcaacgg cgctcgcgct ctcgctatgg
     2821 gaccgcgatt ggcgctggtt gcgcggcaca aggccgttgc cgggcctggt gctcgcggcg
     2881 gcgatcgtcg cgccctgggc gattgcgatc ggccttgcca gccatggggc gttctaccag
     2941 caatccctcg gccacgattt cgcggcgaag attctcggcg gcgaggaatc gcacggcgcg
     3001 cctgccggct actatctgct tcttgccagc ttcaccttct ggcccgccac cgtgtttttg
     3061 atacctgcgc tcggctccgc gattgtccgc cggggcgacc cggtcaaccg ctatctgctc
     3121 gcctggagcg gcgcggcctg gctgatgttc gaactcgtgc cgacaaagct gcctcattac
     3181 atccttccgg tctatccggc actcgcgatt ctgtgcgcgc tctgggccgc cggatcgaat
     3241 cgggaactgg aaacgcgacg aacgtcggtg ctgcactatg ccgccggcgc gcaattcggt
     3301 ctggctgccc tagcgctcgc cgccgcctcg ctggttctgc cgctgcgttt cggcggcgcg
     3361 gtcaattggc tgctgattgc gggcactgcg cttgcatcgg cggcgtcatg ggctgccatc
     3421 tttttcgtga ttcggcagcg cgtggactcc gccgcagctg cagccatcgc atccgcggtc
     3481 gcattttatc tgctcctggc gtttgcggtg gcgccgcaac tccatgatat atggatcagt
     3541 ccgcgcgcgg caaagctcgt cgcggacaac cgccgcccgg acgatcctcc cccggtggtc
     3601 gccggatatg tggaaccaag ccttgtcttt gcgctgggaa gcgacacgcg catcgagccg
     3661 gccgacggcg ctgcccgagc ggcggccgct catggcgggc tggcgctggt cgacggacag
     3721 gctgagccga tatttctccg tgggctcccg tcatttcatg ctgcagcccg gcagctgggt
     3781 cagatctccg gtttcgacta ttcgaacgag cgcaaagagc gagtcattct ctatcgcgtg
     3841 acgccatcgc cgcgaacacc cgcaacaccg gagaattgat gcacagggcc gatccggacg
     3901 acgaagggcg ctccagtttc cggctcgctc ttggaatcgt tgcggcggtt acgctggttc
     3961 gctgtgtcgt gctcgtgctg tcgccgctcg agctctatcc cga
//
LOCUS       DASZMC010000032         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2213815,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000032 DASZMC010000000
VERSION     DASZMC010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213815"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VG274_00615"
     CDS             complement(<1..778)
                     /locus_tag="VG274_00615"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWE05176.1"
                     /translation="MSEVVILSGARTPLGGFQGELSGKSAPELGAVSIAAAVSRAGLT
                     PDQIEETLMGCVLPAGVGQAPARQASRLAGLPDGVPCTTVSKVCGSGMKALMIAADQL
                     SAARAGVLVAGGMESMSNAPYLLPKARGGYRLGHGEIKDHMFLDGLEDAYEHRLMGTY
                     AEDTARQYQFTRGDQDAFAIESLQRAKRAQSDGSFASEIVGVRVSGKGGESEVNTDEQ
                     PRRADPAKIPTLKPAFAKDGTVTAANSSSISDGAAALVVAR"
     gene            complement(799..1971)
                     /locus_tag="VG274_00620"
     CDS             complement(799..1971)
                     /locus_tag="VG274_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HWE05177.1"
                     /translation="MIRNSYPTLDFALGDTADLLRDAVRSFSDDKIAPIADEIDRTNV
                     FPRHLWPQLGALGLLGVTVEEEYGGSGLGYLEHCVAMEEVSRGSAAVGLSYGAHSNLC
                     VNQIRRNGNDEQKRRYLPGLLSGEHVGALAMSESGSGSDVVSMRLRAEKRGDRYLLDG
                     TKMWITNGPEADVLVVYAKTETGAGPRGITAFLIEKSFKGFRAAQKLDKLGMRGSDTS
                     ELVFENCEVPAENVLGREGDGVNVLMSGLDYERAVLAAGSLGIMQACLDVVMPYVHDR
                     KQFGQPIGAFQLIQGKLADMYVTLSSARAYVYAVARACDRGQAARKDAAGAILYAAEK
                     GVWMALEAIQCLGGNGYINDFPTGRLLRDAKLYDIGAGTSEIRRWLIGRELFEQSS"
     gene            complement(2331..2414)
                     /locus_tag="VG274_00625"
     tRNA            complement(2331..2414)
                     /locus_tag="VG274_00625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Leu,seq:gag)
     gene            2545..3405
                     /gene="lipB"
                     /locus_tag="VG274_00630"
     CDS             2545..3405
                     /gene="lipB"
                     /locus_tag="VG274_00630"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWE05178.1"
                     /translation="MDETKEVGKTTSFLPQGGGGGPHGSARSAAGGQAPGRRGTCLPP
                     DVPLRLAALGTSPTLGEEKRASSLRSSPVEWRVEEGLVAYPRAVEFMENRVARIADGL
                     APELIWLIEHPPIYTAGTSAKPSDLLDCRFPVFQTGRGGQFTYHGPRQRVAYVMLDLR
                     LRKADVRAFVRNLEEWIIRTLARFGIAGERRDGRVGIWVVRGNGSEDKIAAIGVRVRR
                     WVTFHGVALNVSPNLDDFGGIVPCGVRGHGVTSLADLGIGASMAGVDAALRAEFENAF
                     GRLHPSTGSG"
     gene            complement(3467..3760)
                     /locus_tag="VG274_00635"
     CDS             complement(3467..3760)
                     /locus_tag="VG274_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWE05179.1"
                     /translation="MAEAEDLTTLRLRIEGHVQAVGFRYYAILEARKLGLNGWVRNLS
                     DGAVEALASGSTKAIEAFVASCMRGPPGALVKNVDLHNAEPPSEPGFHQLPSV"
     gene            complement(3782..>4306)
                     /locus_tag="VG274_00640"
     CDS             complement(3782..>4306)
                     /locus_tag="VG274_00640"
                     /inference="COORDINATES: protein motif:HMM:NF037204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWE05180.1"
                     /translation="PVPPLDAFRVSLGDRELDVSEASMQGGIFRAIVDAGPYEAHVDW
                     HPGCPVMRLAEGGADHVLQMFRVRGGYRVSQGGSSAATVVYGPRAAELASLMPRKELA
                     DVSKLLLCPMPGLVVSVNVLEGQDVKLGETLAVIEAMKMENVLTAEREGKIKKISARK
                     GSSLALDDVIMEFA"
BASE COUNT          784 a         1404 c         1315 g          803 t
ORIGIN      
        1 cccgcgcaac tacgagcgcc gccgcgccgt cggagatcga cgacgaattg gctgcggtga
       61 ccgtgccgtc tttggcgaag gcgggcttga gcgtggggat ttttgccgga tcggcccggc
      121 gtggctgctc gtccgtattc acttcgcttt cgccgccctt gccggagact cttacgccga
      181 caatctcgga cgcgaacgac ccgtcggatt gggcgcgttt tgcccgctgc agcgattcga
      241 ttgcaaaggc gtcctgatcg ccgcgcgtga actgatattg ccgggcggtg tcctccgcat
      301 aggtgcccat gaggcggtgc tcgtaggcat cctcgagccc gtcgagaaac atatgatcct
      361 tgatctcgcc gtgaccaagc cggtaaccgc cgcgtgcctt gggaagcaga tagggcgcgt
      421 tcgacatgga ttccatgccg ccggcgacga ggacgccggc gcgcgccgcg gagagctgat
      481 cggcggcgat catcagagcc ttcatgccgg acccgcagac cttgctgacg gtcgtgcacg
      541 gcacgccgtc cggcagtccg gcgagccgcg aggcctggcg ggcgggcgcc tggccgaccc
      601 cggccggcag cacgcagccc atcagcgtct cctcgatctg atccggcgtc agcccggcac
      661 gcgacaccgc ggcggcgatc gacaccgcac cgagttccgg cgcgctcttg cccgacaatt
      721 cgccctgaaa accgcccaac ggcgtacgcg cgccggacaa aatgacgact tcgctcatgt
      781 cagaaccttc tctgtttgtc agctcgattg ttcgaagagt tcgcggccga tcagccagcg
      841 gcgaatctcg gacgtgccgg cgccgatgtc gtagagcttg gcgtcgcgca gcagccggcc
      901 ggttggaaaa tcgttgatat acccgtttcc gcccaggcac tggatcgctt cgagcgccat
      961 ccacacgcct ttctcggccg cataaaggat cgcacccgcc gcgtccttcc gcgccgcctg
     1021 tccgcgatcg cacgcccggg caacggcgta gacataagcc cgcgcggagg acagcgtcac
     1081 atacatgtcg gcgagcttgc cctgtatcag ctggaatgcg ccaatcggtt ggccgaactg
     1141 cttgcgatcg tgcacataag gcatcacgac atcgaggcag gcttgcatga tgccgagcga
     1201 acccgcagcc aggactgctc gctcgtagtc gagtccgctc atcagcacat tgacgccatc
     1261 gccttcgcgc ccgaggacat tttccgccgg cacctcgcaa ttctcgaaca cgagctcgga
     1321 cgtatcggaa ccacgcatgc cgagcttgtc gagcttctgc gccgcacgga atcccttgaa
     1381 tgatttttcg atcaggaatg ccgtaatgcc gcgcggaccc gcgccagtct cggtcttggc
     1441 atagaccaca agcacatctg cttcggggcc gttggtaatc cacattttcg tgccgtcgag
     1501 caggtagcgg tcgccgcgtt tctccgcccg caggcgcatg gagaccacgt ctgatcccga
     1561 tccgctttcg ctcatcgcca gcgcgccgac gtgctcaccg gaaaggagcc cggggagata
     1621 gcggcgcttc tgctcgtcat ttccattgcg gcggatctgg ttcacgcaca gattggaatg
     1681 ggcgccgtag gaaagtccca cggccgcgga gccgcggctc acctcctcca tcgcgacgca
     1741 gtgttccaga tagccgaggc ccgatccgcc atattcctcc tcgacggtca cgccgagcag
     1801 gccgagtgcg cccaattgcg gccagagatg gcggggaaag acattggtcc gatcgatttc
     1861 atcggcaatc ggggcaatct tgtcgtcgct gaaactgcgc accgcatcgc gcagcaaatc
     1921 ggccgtgtcc ccgagagcaa aatcgagcgt cggataggaa ttgcggatca ttggggatct
     1981 cggagaaatc tcttcatagc cactgctttg ccggagccgg atttgaagta acgctcaagt
     2041 tgccgagctc gcaactctcc ttctatagca acataggcgc gaaggaccgc tggaaggcag
     2101 gtttgcgtcg ccggtactcg cccccggaga tgggtagcga gcctccgacg caagtcgttg
     2161 gtcgaaccga cgtagatgtc gccatgcttc agttgaagaa aatatacgta ccacatggcg
     2221 acggtctccc tacgctcctg cggagcttcg ggagacatca ccctacccct tcgggccttg
     2281 gttggtgtca cccaaggtgg cctgccaccc gaagcccgaa gggcgtaggg tggtgcggtc
     2341 gagaagactc gaacttccac gggttgcccc actagcacct caagctagcg cgtctaccgt
     2401 tccgccacga ccgcgaggcg gggggtttag caaatcgatc ccagcctgtg aagcgcgcga
     2461 gggaggcaat atataaggcg gcggtagcag gacggcgcgc gcctgcgata atgcaattgt
     2521 tctcgcgaaa gcgaattcgc cccgatggat gaaacgaaag aggtcggcaa aaccacatct
     2581 ttcctccccc agggtggggg aggtggcccc cacgggtccg cgcgaagcgc ggccggagga
     2641 caagctccgg gccggagggg gacgtgtctg ccgcctgacg tccccctccg cctcgctgcg
     2701 ctcggcacct cccccacgtt gggggaggaa aagagggcat cgagtttacg ctccagtcct
     2761 gtcgaatggc gcgtcgagga aggtctggtt gcctaccctc gagctgtcga attcatggaa
     2821 aaccgcgttg cccggatcgc cgacggtctg gcgccagaac tcatctggct tatcgagcac
     2881 ccgccgatct acaccgcggg caccagcgcc aagccatcgg atcttctgga ctgccgcttt
     2941 ccggtcttcc aaacaggacg cggtggacaa ttcacctatc acggaccccg ccagcgcgtc
     3001 gcctatgtga tgctcgacct gcggctccga aaagccgatg tgcgcgcctt cgtacgaaat
     3061 ctggaggaat ggatcatccg cacccttgcc cgcttcggga ttgcgggcga gcggcgcgat
     3121 gggcgcgtcg gtatctgggt cgtccgcggc aacggaagcg aagacaagat cgctgcaatc
     3181 ggcgtgcggg tacgccgatg ggtgacgttt cacggcgtcg ccttgaacgt ctcgcccaat
     3241 ctcgatgatt tcggcggcat cgtgccatgc ggcgtgcgcg gacacggcgt caccagcctt
     3301 gccgatctgg gaatcggcgc atcgatggca ggcgtggacg ccgctcttcg ggccgaattc
     3361 gaaaatgcct tcggccgtct gcatccttcg acaggctcag gatgaggtcc ttgagcttgc
     3421 ccgctgcgag tcgaacgatg agatcaggtg gcgcgtatcc gcgatattaa accgacggca
     3481 gctggtggaa gccgggctct gagggtggtt cggcattgtg caggtcgacg ttcttgacca
     3541 acgcgcccgg cggcccgcgc atgcaggagg ctacgaaagc ttcgatcgct ttggtgcttc
     3601 ctgatgcgag cgcttcgact gctccgtccg acaggttgcg gacccagcca ttcaaaccaa
     3661 gcttgcgcgc ttccaggatc gcgtagtagc gaaagcccac cgcctgtacg tgaccctcga
     3721 tccgcaggcg gagcgtcgtc aggtcttcgg cttcagccat tttccggctc cagcaaggcg
     3781 atcatgcgaa ctccatgatg acgtcatcga gcgcaaggct tgagcccttc ctggcgctga
     3841 tcttcttgat cttgccctcc cgctccgcgg tcagcacgtt ttccattttc atcgcttcga
     3901 tgactgcaag tgtttcgccc agcttcacgt cctggccttc caggacattg acggagacca
     3961 ccaggcccgg catcgggcaa agcagaagct tcgacacgtc cgcgagttcc ttcctgggca
     4021 tcaacgatgc aagctcggct gcccgcggcc cgtagaccac cgttgcggcc gacgatcccc
     4081 cctgggacac gcgatatcct ccccgcaccc ggaacatctg aagtacgtga tcggcgccgc
     4141 cttcggccag ccgcatgacc gggcagccgg gatgccaatc gacatgcgcc tcgtatggtc
     4201 ctgcatctac gatcgcgcgg aatattcccc cctgcatgct ggcttcggac acgtccagtt
     4261 cccggtctcc aagcgatacc cggaacgcat caagcggcgg cacggg
//
LOCUS       DASZMC010000033         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4141614,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000033 DASZMC010000000
VERSION     DASZMC010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4141614"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..753
                     /locus_tag="VG274_00645"
     CDS             100..753
                     /locus_tag="VG274_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05181.1"
                     /translation="MTGPAFLLGLPALREGPLWTTARELGPPVLISANALSRWTVDRQ
                     GYRIWTGFDPRALHLVSSHPVALDSAGFVAAHRYRGFAWETDDYLDLAASAPWIWWAS
                     QDWCVEPEIAGDEDAVLDRISGTVRFNLRCLNGAKRRGIAHNFVPVIQGWRPEHYLRC
                     FDRMPFALDAPLIGVGSMCRRHVGGPNGILSVLRVLDDAFAGTNTRFHLFGLKSQGI"
     gene            775..1095
                     /locus_tag="VG274_00650"
     CDS             775..1095
                     /locus_tag="VG274_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05182.1"
                     /translation="MASCDSQAYGVAARQDAWKARVAKSDRYVAATMRRWYENQVATM
                     RIPPARVMQADAAAPETWTARSASEERIAEAYEEMRALHEAGEIEWSQLSSLLAYEMA
                     FLDD"
     gene            1461..>1657
                     /gene="ssb"
                     /locus_tag="VG274_00655"
     CDS             1461..>1657
                     /gene="ssb"
                     /locus_tag="VG274_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWE05183.1"
                     /translation="MAGSVNKMILIGNLGRDPEVRSTQGGDKIVNLSIATSESWTDKR
                     TGDRRESTEWHRVVIFSNGLA"
BASE COUNT          321 a          547 c          497 g          292 t
ORIGIN      
        1 accgaccgaa ccgcagatca atgcgatgaa gcatgggtcg atgttcggct gggacacacc
       61 cggtgcccgc cccgagttct ggcggcacag gtctaccctg tgaccggtcc ggcattcctt
      121 ctcggcctgc ccgccttgcg agaaggacca ctctggacga ctgcgcgcga actcgggcca
      181 cccgtcctga tcagcgcgaa tgcgctgtca cgatggacgg tcgatagaca gggctatcgc
      241 atatggacgg gcttcgatcc gcgcgcgctc catctcgtct cttctcaccc ggttgccctc
      301 gattccgcgg ggttcgtcgc cgcgcaccgc taccgcggct ttgcgtggga gaccgacgac
      361 tatctcgacc ttgcggcctc cgcaccctgg atttggtggg ccagccagga ttggtgcgtc
      421 gaacccgaaa tcgcgggcga cgaagatgcc gtgctcgaca gaatttccgg caccgttcgg
      481 tttaatctgc gatgcctcaa cggcgcaaaa cggcgcggca tcgcccacaa cttcgtgcct
      541 gtgatccaag gatggcgccc cgagcactac ctgcgctgct tcgatcgcat gccgttcgcc
      601 ctcgatgctc cgctgatcgg cgtcggcagc atgtgccggc gccatgtcgg cggcccgaac
      661 ggtatcttga gtgtcctgcg cgtgctcgat gacgcattcg ccggcacgaa cacgcgcttt
      721 catcttttcg gcctgaaatc ccagggcatc tagcacgcgg cagcgcatgt gcgcgtggcc
      781 agttgcgatt cccaggccta cggtgtggcc gcgcgccagg atgcatggaa agcgcgcgtt
      841 gcgaaatcgg atcgctatgt cgccgccacc atgcgccgtt ggtacgagaa ccaggtcgcg
      901 acgatgcgca tccctccggc tcgcgtcatg caagctgatg cagcggcgcc ggaaacctgg
      961 actgcaagat cagcgtccga agaacgcatc gccgaagcct atgaagaaat gcgcgccttg
     1021 cacgaggcag gcgaaatcga atggtcgcaa ctctcctcgc tgcttgcgta tgagatggcg
     1081 ttcctcgacg actagccttc ggcgacgccc ggcctactgc caccaagcgc ccacaaccat
     1141 aagtaaaacc gaaattgcaa cctttgaaaa ttcaaaggaa gaagggggga tgtatgtcga
     1201 gaatcccagc acctcgcggg cctcgttccc ctctcgaata agcgcggtct aaaccaggac
     1261 cgttcccgct tccgcatccc cgcagcgcca aggcgctgcg ccttcggctc gtagcgctgc
     1321 cgtgtcgctt cgctcccgca ccaagccgcg ccactcgggg gcgggcgggc ccagccccgg
     1381 ttttctcaga ccgcatccga gaggacgaag cccggcgcag tgccgggcac tcctcccagg
     1441 aatgaaaaag gaatgagaca atggctggtt cagtgaacaa gatgatcctg attggcaatc
     1501 ttggccgtga cccggaagtg cgcagcaccc agggcggcga caaaatcgtg aacctctcga
     1561 ttgcgacaag cgagagctgg acggacaagc gcaccggcga ccggcgcgaa agcaccgaat
     1621 ggcaccgcgt cgtgatcttc agcaatggcc tcgccga
//
LOCUS       DASZMC010000034         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4411002,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000034 DASZMC010000000
VERSION     DASZMC010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4411002"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..1658)
                     /locus_tag="VG274_00660"
     CDS             complement(291..1658)
                     /locus_tag="VG274_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05184.1"
                     /translation="MSDAVEISFRADLAELESGAAEAVSLLRNAAAQMAAAFVPNPRE
                     ADAAQHIADERIKIAERASREEIATAEEKNNFLLRMGEESLDQWKSQAVELETARTEI
                     AQEGLKKREAADTNDKLALARDQGEEELLAAEHANRLARIDEQYREKQKQIDQTDLQD
                     FIKTSDDRLNAAIAKLAEEYKAHQIGAEEKHAAEIGLTLEIEAEVLKRFDAENAGLVK
                     GTDAYAKAMKDRQGLLDRFNKHVVTADNQLEQEESQKWTQLGHSIESSFNSALDGLIF
                     HGETFKQFMGQVAQGVLEAFLHMGEQVLENWIETQTEQMFLTRATSSATALGQITDNA
                     AVAASGAAAATAQIPYVGPVLAPAAAASTFADTMSWASALAFDVGAWEIPDTMRAILH
                     KGEMVVPENFASGLRGNAGGLGGGQHFNVENTFNHHGSGAWNAEDHARDIVRVLKTQL
                     RLGVR"
     gene            complement(1655..2017)
                     /locus_tag="VG274_00665"
     CDS             complement(1655..2017)
                     /locus_tag="VG274_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05185.1"
                     /translation="MKSDEIPAIVLAGREWPIPLLAPRQNRIVVPALLELIPRILAAR
                     AQADAAGESGQISQLARTLDTAFYDRLGDAVFTALTRAEPGLTRAAFDDMAIDTLELL
                     VAVRVIALQAGLLKRGAR"
     gene            complement(2040..2603)
                     /locus_tag="VG274_00670"
     CDS             complement(2040..2603)
                     /locus_tag="VG274_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05186.1"
                     /translation="MNDPTITIAGREWRIPPLAPRQNRVVLPGLMRLGKEPAEQYGTL
                     LDIVFAALTRAHPSMTREEFDDWPIATWELVESIPVIARQTGLLRSSFDRLRTRFPPH
                     AELGEAPDWDAIIAQYVNFLPGTTPDYWEDALTATRLDAMQAEWRRHPPLALLVAGAL
                     GYRPPARDSDALEELLQLFSNGKLRLN"
BASE COUNT          441 a          901 c          875 g          562 t
ORIGIN      
        1 ctgccacacc cccggcatcg catattgcag gaagccggag cgggcgagga aatcggctgt
       61 ggcggcgtaa tggtcgaagc cgtcccagat tttcaaggcc atccgtgttg ccttttaacg
      121 cagaggccgc ggaggatgcg cagaggacgc ggggggaaag gaagattcac aatgagacgg
      181 tgaggacagt gagagacttt ttgcgcgctt cgcgcgcgat cattcttccc cagcgtccac
      241 tgcgcgtccc ctgcgtaccc tgcgttaaag ttttcttcct tccgccgtca tcaccgcacg
      301 cccagccgca gctgggtttt cagcacgcgg acgatgtcgc gggcgtggtc ttcggcgttc
      361 cacgcgccgg agccgtgatg attgaaggtg ttttcgacgt tgaaatgctg gccgccgccg
      421 agtccgcccg cattgccgcg caatccgctg gcgaaatttt cgggcaccac catctcgccc
      481 ttgtgcagga tcgcccgcat ggtgtcgggg atttcccagg cgccgacgtc gaaggcgagc
      541 gccgaggccc agctcatcgt gtcggcgaag gtggatgccg ccgccgcggg cgccagcacc
      601 ggcccgacat agggaatctg cgccgtcgcc gccgcggcgc ccgacgccgc caccgcggca
      661 ttgtcggtga tctggcccaa agccgtcgcc gacgaggttg ccctggtgag gaacatctgc
      721 tcggtctggg tctcgatcca gttctccagc acctgctcgc ccatgtgcag gaaagcttcg
      781 agcacgcctt gcgcgacctg gcccatgaac tgcttgaatg tctcgccgtg gaatatcagg
      841 ccgtcgagcg cggagttgaa gctcgattcg atggaatggc cgagctgggt ccatttctgc
      901 gactcttcct gctcaagctg gttgtcggcg gtcacgacat gtttgttgaa ccggtcgagg
      961 agcccctggc ggtccttcat cgctttggcg taggcatcgg tgcccttgac gagcccggcg
     1021 ttctccgcgt cgaagcgctt gagcacttcg gcctcgattt cgagggtcag cccgatttcg
     1081 gccgcgtgct tctcttccgc gccgatctgg tgggccttgt actcctcggc gagcttcgca
     1141 atcgccgcgt tcagccggtc gtcggaggtt ttgatgaaat cctgcaggtc ggtctggtcg
     1201 atctgcttct gcttctcccg atattgctcg tcgatccggg cgagtcggtt cgcatgctcg
     1261 gcggcgagca gttcctcttc gccctgatcc ctggccagcg cgagcttgtc gtttgtgtcc
     1321 gccgcttcgc gcttcttcag tccctcctgg gcaatctcag tgcgcgcggt ctcgagctcg
     1381 acggcctgcg acttccattg gtcgagcgat tcctcgccca tgcgcaggag gaaattgttc
     1441 ttctcctccg cggtcgcgat ttcctcgcgc gaggcgcgct ccgcgatctt gattctctcg
     1501 tcggcgatgt gctgggccgc gtccgcttcg cgcggattcg gcacgaaggc ggccgccatt
     1561 tgcgccgcgg cgttgcggag cagcgaaacc gcttcggccg ctccgctctc cagttccgca
     1621 agatcggcgc ggaaggaaat ctcgaccgcg tcgctcatcg cgcgccccgc ttcaacaggc
     1681 cggcctgaag cgcgatgacc cgcaccgcaa ccagcagctc gagcgtgtcg atggccatgt
     1741 cgtcgaacgc ggcgcgcgtg agcccgggct cggcccgcgt cagcgcggtg aacaccgcgt
     1801 cgcccagccg gtcgtagaag gcggtgtcga gggtgcgtgc gagctgcgag atttgtcccg
     1861 attcccccgc tgcatcggct tgcgcgcgcg cagcgaggat tcgcggtatc aattcgagca
     1921 gcgccggcac gacgatccgg ttctgcctcg gcgcgagaag cgggatcggc cattcgcgcc
     1981 cggcaagaac gatcgcggga atctcgtcgg atttcatgtc ttgctccggg tgcgcggttt
     2041 caattcaacc gcagctttcc gttcgaaaag agctgcagca attcctccag cgcatcgctg
     2101 tcgcgcgcag gcggccggta gccgagagcg ccggccacca gcagcgcaag cggcggatgc
     2161 ctgcgccatt ctgcctgcat cgcatcgagc cgcgtggcgg tcagcgcatc ctcccagtaa
     2221 tccggcgtgg tgccgggaag aaagttcaca tattgcgcga tgatcgcgtc ccagtcgggt
     2281 gcttcgccaa gctcagcatg aggaggaaac ctcgtcctga gcctgtcgaa ggacgaacga
     2341 agcaatcctg tttgtcttgc tattaccgga atgctctcca caagctccca ggtcgcaatc
     2401 ggccagtcgt cgaattcctc gcgcgtcatg gacggatggg cgcgggtcag agccgcaaag
     2461 acgatatcga gcagcgtgcc gtattgttcc gccggttctt tgccaaggcg catcagcccc
     2521 ggcagcacga cacggttctg ccgcggcgcg agcggcggaa ttctccattc ccgcccggcg
     2581 atggtgatgg tcggatcgtt catggaagga aaactttaac gcagaggacg cagaggatgc
     2641 gcagtggacg ccgacaaaaa atggttgcgc gccgacggcg cgccattctt gttcccccgc
     2701 gtcctctgcg caccctctgc gcccactgcg ctaaaatctt gttccttatt cctactcgct
     2761 gaaggcgaac gtgccccag
//
LOCUS       DASZMC010000035         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4685873,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000035 DASZMC010000000
VERSION     DASZMC010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4685873"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..565)
                     /locus_tag="VG274_00675"
     CDS             complement(203..565)
                     /locus_tag="VG274_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05187.1"
                     /translation="MLIKKTLMAVVAAFTLIGATGAMAMDRDHDGRPDRSWAEKHDRD
                     GYRDVRHDRYWKPGFGNYIARDRVFVTLRGRGYNRFVGDPYFYQGRYVVRSYNRFGKI
                     VFVEVNPYTGGFIGEVRL"
     gene            713..1117
                     /locus_tag="VG274_00680"
     CDS             713..1117
                     /locus_tag="VG274_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05188.1"
                     /translation="MRKSVIFWILAAFLAFDAGSAQADYYAPASLDRILPQVRSHHPG
                     KFYDAEGPYRGPDGQFRYRLKWMTPGGRIVWFDADARTGRVLGPDSEMRVAPYFRDGP
                     RFDEPRDFRDDRRWNRGGRWPREDRPRGGHRR"
     gene            1125..1814
                     /locus_tag="VG274_00685"
     CDS             1125..1814
                     /locus_tag="VG274_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWE05189.1"
                     /translation="MRLLLVEDDRDLQRLLKKALSDAGYVVDTASDGEEGHFLGDTEP
                     YDAVILDLGLPKLDGVRVLEKWRKDGRSMPVLILTARDRWSDKVAGFDAGADDYLAKP
                     FYTEELLARVRALLRRAAGFATSEIEIGKLHIDTRASRVTMDGNPVKLTSLEYRLLAY
                     LAHHRGKVVSRTELVEHLYDQDFDRDSNTIEVFVGRLRKKLDSNLIQTVRGLGYCLEV
                     PDAGVKTNAQA"
     gene            1846..3174
                     /locus_tag="VG274_00690"
     CDS             1846..3174
                     /locus_tag="VG274_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWE05190.1"
                     /translation="MIWTTLGLVIGGFFLSNAFRSAVQDNFDTTLQVDLDGMIAAAEP
                     DPEGGVVLEERFLNRRFERVYSGLYWQITPLDPGQKDVQISHSLFDHSIHIDNLRKNG
                     NLTWGYSEGPDGQRLRVLEQRVEFPITATARPDDSRAYTFLVAGNTKEVEDEINDFNG
                     TLIWAFSLLGAGLIIAIFLQVRIGLLPLRRVRDSLARIRDGKARRLEGKFPAEIAPLA
                     GELNSLIQHSEEVVGRARTHVANLAHFLKTPLTVLSSEAEAQPGPLADAVKRQVDSMR
                     RQVDHYLVRGRAAGALDVLGNRTKVKAVLEDLARVLERIHEARGIEIEVNCSPSLFFR
                     GDRQDLEEMSGNLIDNACKWAKHRVTVSAAAVAGARWELAIEDDGPGLAAEDRERVLE
                     RGERLDESVPGSGLGLSIVEDIAKLYGGSLELGTSPMGGLAARLGLPAII"
BASE COUNT          635 a         1033 c         1049 g          666 t
ORIGIN      
        1 ctctgcatcg aaaatccccc ggcccaagtc ccggcacgat gctaccagcc ctgatgcacc
       61 agtccaagcg ggatgcagaa atccgccccg ccaaaaatga gaaacccgcg ccggggaagt
      121 ccggcgcggg ctctcgcttg acggtgtgcg gccctatggg gaggggctta ccacgaaccg
      181 cacgcctcaa gagctggaca gcctagagac ggacctcgcc gatgaagccg cccgtgtagg
      241 ggttcacttc gacgaacacg atcttgccga aacggttgta gctgcggacc acataacggc
      301 cctgatagaa gtacgggtcg ccgacgaagc ggttgtagcc acggccacgc agtgtcacga
      361 acacacggtc gcgcgcgatg taattgccga agcctggctt ccaatagcgg tcgtggcgca
      421 catcgcgata tccgtcgcga tcatgcttct cggcccagct gcggtcgggg cggccgtcgt
      481 gatcgcggtc catcgccatt gcgccggtgg cgccaatcag ggtgaatgcg gcaaccactg
      541 ccatcaaagt cttcttgatc aacatatctt actcctcttt cttcaagttt cgttttggga
      601 gcggtccaac tggagccgcc gttccatgtc acgtaagatg gcagggcggt cctgaatgcg
      661 ttctggacgg catgttcatt cgaggttcat ttgcgcggca gtaaaaaaca gcatgcgtaa
      721 gagtgtaatt ttctggattt tggcggcttt tctggctttt gacgccggtt ccgcgcaggc
      781 ggattactat gcgccggcgt cactggaccg catcctgccg caggtccgca gtcaccatcc
      841 cggcaaattc tacgatgccg agggcccgta tcgcggaccc gacggacagt ttcgctatcg
      901 cctgaaatgg atgacgccgg gcggacgcat cgtctggttc gatgccgatg cgcgcaccgg
      961 acgcgtgctt ggtcccgatt ccgaaatgcg cgtcgcgcca tattttcgag acggtccgcg
     1021 cttcgatgag ccgcgcgatt tccgcgacga ccggcgttgg aatcgtggcg gacgctggcc
     1081 gcgcgaagat cgtccacgcg gcggacaccg cagatagggg aatcatgcgc ctgttgcttg
     1141 tcgaagacga ccgcgatctg cagcgccttc tcaaaaaagc gctgagcgat gccggctatg
     1201 tcgtcgatac cgcgagcgat ggcgaagaag gccatttcct cggcgacacc gaaccttacg
     1261 atgcggtgat cctcgatctt ggtcttccga aactcgacgg cgtgcgtgtg ctggagaaat
     1321 ggcgcaagga cgggcgctcg atgccggtac tcattctcac tgcgcgcgac cgctggagcg
     1381 ataaggtcgc gggcttcgat gccggcgccg acgattatct cgccaagcct ttttatactg
     1441 aggagttgct cgcccgcgtg cgcgcgctgc tgaggcgcgc ggcgggtttc gcgacgtcgg
     1501 aaatcgagat cggaaagctg cacatcgata cgcgtgccag ccgcgtgacg atggacggca
     1561 acccggtgaa gctcacctcg ctggaatacc gcctgctcgc atatctggcg catcatcgcg
     1621 gcaaggtcgt ctcgcgcacc gagctcgtcg agcaccttta cgatcaggat ttcgaccgcg
     1681 actcgaatac gatcgaggtg ttcgtcggtc gcctgcgcaa gaaattggat tccaatctca
     1741 ttcaaacggt gcgtggtctt ggctactgcc tcgaagttcc ggacgctggc gtcaagacta
     1801 acgcccaggc ttgattctct cgccgcgcgg ctgatcgcgg cagctgtgat ctggaccacg
     1861 cttggtctgg tgatcggcgg attttttctg tccaacgcat tccgctctgc cgtccaggac
     1921 aatttcgaca cgaccttgca ggtcgatctt gacggcatga tcgccgccgc agagcccgat
     1981 ccagaaggcg gcgtggtact cgaagagcgt ttcctcaatc gtcggttcga acgcgtctat
     2041 tctggtctct attggcagat caccccgctc gatccgggac agaaggatgt ccagatttcc
     2101 cactccctgt tcgaccattc gatccacatc gacaatctgc gcaagaacgg gaacctgacc
     2161 tgggggtatt ccgaaggccc ggacggacag agattgcgcg tgctggagca gcgcgtcgaa
     2221 ttcccgatca cggcgaccgc gcgtccggac gacagccgcg cctatacgtt cctcgtcgcg
     2281 ggcaacacga aggaagtcga agacgagatc aacgatttca acggcaccct gatctgggcg
     2341 ttctcgctct tgggcgcggg attgatcatc gcgatcttcc tgcaggtgcg catcgggctt
     2401 ttgccgctgc gccgcgtgcg ggattcgctc gcgcgcatcc gtgacggcaa ggcgcgccgg
     2461 ctcgaaggca agtttccggc ggagatcgcg ccgctggcgg gtgaactcaa cagcctgatc
     2521 cagcacagcg aggaagtcgt cggccgcgcg cgcacgcatg tcgccaacct tgcgcatttt
     2581 cttaagacgc cgctcaccgt tctgtcgagt gaggcggaag cacagccggg cccgctcgcc
     2641 gatgcggtga agcgccaggt cgattccatg cgccgacaag tcgatcacta tctcgtgcga
     2701 ggccgggcgg ccggcgcgct cgacgtgctc ggcaaccgca cgaaggtgaa ggccgtcctc
     2761 gaggatctcg cgcgcgtact cgagcggatt catgaagcgc gcggcatcga gatcgaggtg
     2821 aattgctcgc cctcgttatt cttccgcggc gatcgtcagg accttgagga gatgtcgggc
     2881 aacctcatcg acaatgcctg caaatgggcc aagcaccgcg tcacggtcag cgcggcggcg
     2941 gttgccggcg ccagatggga actcgcaatc gaggacgacg gaccgggctt ggcagccgaa
     3001 gatcgcgaac gtgtgctgga gcgcggcgaa aggctggacg aaagcgtgcc gggttcgggc
     3061 ctcggccttt ccatcgtcga ggacatcgcc aagctttatg gcggttccct ggaactcggc
     3121 acctcgccga tgggcggatt ggcggctcgc ctcggcctcc ctgccataat atgacaacgc
     3181 tgcgttaacg gcgctgggat aacctgtaga ggcgaattcc aaacggatcg actgcgaatt
     3241 ttctcatgct gacgccaagg acatcgacgc ttcttcagag cttcctgcgc gcgttgccgc
     3301 aatcggcgag cgcgcgtttg gtgaaggccg tcgaaatgga tcgtctttcc ggcggacgcg
     3361 tgcttccgca cgatctgatc ctt
//
LOCUS       DASZMC010000036         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2216501,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000036 DASZMC010000000
VERSION     DASZMC010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216501"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VG274_00695"
     CDS             complement(<1..1298)
                     /locus_tag="VG274_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05191.1"
                     /translation="MNAILFAVLFAAGCTSTHVREPLPAGWAADDSHARTEFWYSLTS
                     RPVAANDEVFHGLLLYLDGSDKCVDYAARVKEMQSRKMLPADFRGAADEAADRGTLAY
                     ALVRVLKIDGGLTMHLFGDSPRYALRALEYRGIYPTCSANQGISGAQFVGIMQKAEEY
                     ERGNPADAPAVKLPDEIRHEGGALAALDKKAGPPNDRVRFHDGEFAGSAPRTVMWGER
                     ESVRDADPTRIASATNDLLSAVRGGEGSGGGPFREFAQSADLDGRPVAALAPFTLLDA
                     RPLTPALSPEYDSTALAAGRGEGADAGHVAIYLNDISAEDARALFLADAEPAVAPAEA
                     PASKAPKKLNVIITGVQGDSVEARKTEKDPWTKARVGLVLHEAAEFRTGPKSAVRFII
                     PPDQTFCLDSQGTTKVLDAVANGNKVKTDIGLSRGRMRYDV"
     gene            complement(1471..>1944)
                     /locus_tag="VG274_00700"
     CDS             complement(1471..>1944)
                     /locus_tag="VG274_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWE05192.1"
                     /translation="DRRRTKFTAEGVLASGDKDRTVTAGTFGGNAPHTRDGAFNSLGV
                     IYDGLAFTPPISNLMVLRVGASTYPVPDGALRGLQVGADFFVLGKARIAAPIDEPTNN
                     SRYLGCEPDVFINWQITDDVSFALRYGLFFPGEAIPSGDQDHVRQFLYAAVTYAF"
BASE COUNT          360 a          621 c          599 g          364 t
ORIGIN      
        1 acgtcatagc gcatgcgccc gcgcgacagg ccgatgtccg ttttcacttt attgccgttg
       61 gccaccgcgt cgagcacttt ggtggttccc tggctgtcga ggcagaaggt ctggtcgggc
      121 gggatgatga accggaccgc gctcttgggc ccggtgcgaa actcggcggc ttcgtgaagc
      181 accaggccga cgcgcgcctt ggtccacgga tccttttcgg ttttgcgcgc ttctacggaa
      241 tcgccttgca cgccagtgat gataacgttg agcttcttcg gcgctttact ggctggagcc
      301 tccgccgggg cgacggcagg ctcggcatca gccaggaaca gggcgcgggc gtcttcggcg
      361 gaaatatcat taaggtatat cgcgacatgg ccagcatccg ctccctctcc cctgcctgcg
      421 gcgagcgcag tcgagtcgta ctcaggggag agggctgggg tgaggggccg ggcgtcgagg
      481 agcgtgaagg gcgcgagagc tgcaaccggc ctaccgtcca aatctgcgga ttgcgcaaac
      541 tcccgaaatg gccctccccc tgagccctct cctccgcgta cggccgagag caagtcgttc
      601 gtggcgctcg cgattcgcgt agggtccgca tcgcggaccg attcgcgctc accccacatc
      661 acggtccgcg gtgcggaccc tgcaaattcg ccgtcgtgaa atcgaacgcg atcgttcggc
      721 ggaccggcct ttttatccaa cgccgccagc gccccgccct cgtggcggat ctcgtcggga
      781 agcttgaccg ccggcgcgtc ggcgggattt ccgcgctcgt attcttcggc tttctgcatg
      841 atgccgacaa actgtgcgcc ggagattccc tggttcgcgc tgcacgtcgg ataaatcccg
      901 cggtattcca acgcccgcag ggcatatcgc gggctgtcgc cgaacaggtg catcgtcagg
      961 ccgccatcga ttttcaacac gcgaacgagg gcataagcaa gcgtgccgcg gtcggcggct
     1021 tcgtcggcag cgccgcggaa atccgccggc agcatcttgc gcgattgcat ttccttcacg
     1081 cgggccgcgt agtcgacaca cttgtccgag ccgtcgagat agagcagcaa gccgtggaac
     1141 acttcgtcat tggcggcaac cggccggctg gtcagtgaat accagaactc cgtgcgcgcg
     1201 tgtgaatcat ccgccgccca acctgcgggc aaaggttcgc gaacatgagt cgaggtacaa
     1261 ccagcggcga aaagaacggc gaacagaatt gcattcaata tccatgcgaa gccgctaagt
     1321 cgtggcacgg gcggcccgcc cgtgtttttg cgaccagaac atgggcgggc cgcccatgcc
     1381 acgtcgagaa cctcttcgcc ccttcgcgcc ttcgtggcaa gtctttcgca cctgagttcg
     1441 acagacgtgc ggccgagatg tttcatgtga tcaaaatgca tacgtcaccg ccgcgtaaag
     1501 gaattgccgg acgtggtcct ggtctcccga cggaatcgct tcgcccggga agaacaggcc
     1561 gtagcgaagg gcgaagctca cgtcatccgt gatctgccaa ttgatgaaaa catcgggctc
     1621 gcatccgaga taccggctgt tattggtcgg ctcgtcgatc ggcgccgcga ttcgggcttt
     1681 gccgaggacg aagaaatcgg cgcccacctg caagccgcgc aacgctccat ccggcacagg
     1741 atatgtcgac gcgccgactc gcaacaccat caaattcgag atcggcggcg taaaagccag
     1801 accatcataa atcacgccaa ggctgttgaa agcgccgtct ctcgtgtgcg gagcattgcc
     1861 gccaaacgtg ccggcggtca cagtccggtc cttgtcgccc gaagccagca ccccttcggc
     1921 ggtgaacttc gtgcgccgcc gatc
//
LOCUS       DASZMC010000037         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_15183,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000037 DASZMC010000000
VERSION     DASZMC010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15183"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VG274_00705"
     CDS             complement(<1..268)
                     /locus_tag="VG274_00705"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWE05193.1"
                     /translation="MSEEPHVLFEKRGAAGLITLNRPKALNALTHGMCLQMTMQLRAW
                     ATDDDVKVVVVQGSGERAFCAGGDIRSLYESGQNKTPYALNFYRV"
     gene            complement(434..1573)
                     /locus_tag="VG274_00710"
     CDS             complement(434..1573)
                     /locus_tag="VG274_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isobutyryl-CoA dehydrogenase"
                     /protein_id="HWE05194.1"
                     /translation="MDFSLNEDERAIEDAARRFSRDRLAPFAAEWDETAHFPVDRLRE
                     AAALGFAGIYVKSDVGGSEMSRLDAAIIMEELSAGCTSTAAFISIHNMASWMIDRYGS
                     EAQRQKFLPSLTTMEKIASYCLTEPGSGSDAAALKTRAVKDGDHYVLNGSKAFISGAG
                     VSDIYVCMVRTGEDGPKGISCLVIEKGTPGLSFGKAEKKMGWNSQPTATVNFSDCRVP
                     VANRLGAEGEGFRIAMSGLDGGRINIGACSVGTARAAIEEAKTYVTERKQFGKPIADF
                     QVTQFKLADMATELEASRLMIRSAASALDKRDPRATMLCAMAKRFATDIGSKIANDAL
                     QLFGGYGYLKDFPAERHVRDLRVHQILEGTNEIMRVIVSREMFRQ"
     gene            1706..2716
                     /gene="hemB"
                     /locus_tag="VG274_00715"
     CDS             1706..2716
                     /gene="hemB"
                     /locus_tag="VG274_00715"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005179750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWE05195.1"
                     /translation="MASDPSFPALRMRRLRRHEWSRRLVAESTLTPADFIWPLFVIDG
                     QNTRQPIASMPGVERLSVDLAVEAAKEARDLGIPVIALFPNTDPDLKTEDGREATNDN
                     NLVCRAVRAIKKAVPEIGVLCDVALDPYTSHGHDGVLSGDYVTNDESVAILVKQALVQ
                     AEAGCDIIAPSDMMDGRVGAIRTALEAKGFRNTMIMAYAAKYASAFYGPFRDAVGSAK
                     ALTGDKRTYQMDPANGDEALREVALDLAEGADMVMVKPGMPYLDVLRRVKDEFGVPTF
                     AYQVSGEYSMLTGAIERGWFDRDRVMMESLMAFKRAGASGILTYFARDAARLLKKGSS
                     AI"
     gene            2746..>3200
                     /locus_tag="VG274_00720"
     CDS             2746..>3200
                     /locus_tag="VG274_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWE05196.1"
                     /translation="MRFLSRRYWRTRVVFWGGAITVGLVSVVFAKAANYAQALFAHVL
                     VNPWIALGLTPAGFVFSVWLARAVFPGSQGSGIPQAIAARELDEPSARKKLLSLKIAF
                     GKILLTLVGLASGASIGREGPTVQVGAAIMLFTARVGDAALERGLILAGS"
BASE COUNT          575 a         1051 c          982 g          592 t
ORIGIN      
        1 cgacgcgata gaaattcagc gcatagggcg tcttgttctg cccgctctca tagagcgagc
       61 ggatatcgcc gccggcacag aacgcgcgct cgccggagcc ctgcacgacc acgaccttca
      121 cgtcgtcgtc cgtagcccag gccctgagct gcatcgtcat ctgcaaacac atgccgtgcg
      181 tcagcgcgtt cagcgccttc ggcctgttca gggtgatcag accggccgcg ccacgctttt
      241 cgaaaagaac gtgaggttct tcagacatgg ccgaccagtt ggggatgcga aactcccccc
      301 ccgttcacgg gggaagtgga ccgacgacgc aacgcatcga cgggacgaag ggggaatgcc
      361 gccagagtcc cccccctcgc cgccttcggc ggctctcccc ccgtaaacgg ggggagattg
      421 ttgtgcatcg aaatcactga cggaacattt cccgggacac gatcacgcgc atgatctcgt
      481 tggtgccttc gaggatctgg tgcacgcgca gatcgcgcac atggcgctcg gcggggaaat
      541 ccttgagata gccgtaacca ccgaagagtt gcagcgcatc gttggcgatc ttcgagccta
      601 tgtcggtggc gaagcgcttc gccatcgcgc acagcatcgt cgcgcgcgga tcgcgcttgt
      661 cgagcgccga cgccgcgctg cggatcatca gccgcgaggc ttccagttcc gtcgccatgt
      721 cggcgagctt gaactgcgtc acctgaaagt cggcgatggg ctttccgaac tgcttgcgct
      781 cggtgacata ggtcttggct tcctcgatcg cggcgcgcgc cgtgccgaca gagcaggcgc
      841 cgatattgat gcgtccgccg tcgagcccgc tcatcgcgat gcggaaaccc tcgccttcgg
      901 cgccgaggcg attggcaacc ggcacacggc aatccgagaa gttcaccgtc gcggttggct
      961 gcgaattcca gcccatcttt ttttcggcct tgccgaacga gaggccgggt gtgcccttct
     1021 cgatcacgag acaggagatt cctttcggcc cgtcctcgcc ggtgcgcacc atgcagacat
     1081 agatgtcgga cacgcccgcg ccggagatga acgccttcga gccgttcagc acataatgat
     1141 cgccgtcttt caccgcgcgc gtcttgagcg cggcggcgtc cgaacccgag ccgggctcgg
     1201 tcagacaata agacgcgatc ttctccatcg tcgtgagcga cggaaggaac ttctggcgct
     1261 gcgcctcgct tccgtagcgg tcgatcatcc acgacgccat gttgtggatg gagatgaacg
     1321 ccgccgtgga cgtgcagccc gcggacagtt cctccatgat gatcgccgcg tcgagacgcg
     1381 acatctccga cccgccgaca tcgctcttca catagatgcc cgcgaagccc agcgccgccg
     1441 cttcccgcaa ccgatcgacc gggaaatggg cggtctcgtc ccattccgcc gcaaagggcg
     1501 ccagccggtc gcgcgaaaac cgcctagctg cgtcttcgat ggcccgttcg tcctcgttca
     1561 gggagaaatc catggcgcaa ttcctgcgaa atcggggcgt aattaacccc ctgaagcggc
     1621 agatgcaaag gctgcaaatt cgccgccttg cggggccatg cgcccctcta gtagaccctt
     1681 gagccgcctg cccggaactt cgttcatggc ctcagatccg agttttcccg cgctccgcat
     1741 gcgccgcctc cgccgtcatg agtggtcgcg ccgtctggtc gcggaatcga cgctgacgcc
     1801 ggccgatttc atctggccgt tgttcgtgat cgacgggcag aacacgcgcc agcctatcgc
     1861 ctcgatgccc ggcgtggaac gcctaagcgt cgatctcgcg gtggaagcgg cgaaagaagc
     1921 gcgcgatctc ggtatcccgg tgatcgcgct gttccccaac accgatcccg atctcaagac
     1981 cgaggacggc cgcgaggcga cgaacgataa caatctcgtc tgccgcgccg tgcgcgcgat
     2041 caagaaagcg gttccggaga tcggcgtgct ctgtgatgtc gcactcgacc cttacacctc
     2101 tcacggtcac gacggtgttc tgtccggcga ttacgtcacc aatgacgaaa gcgtcgcgat
     2161 cctggtgaag caggccctag tccaggccga agccggatgc gacatcatcg cgccatccga
     2221 catgatggat ggccgcgttg gcgcgatccg tacagcgctc gaggccaaag gcttccgcaa
     2281 cacgatgatc atggcctacg cggcaaagta tgcgagcgcg ttctacggtc cgttccgcga
     2341 cgcggtcgga tcggccaaag cgctgaccgg cgacaagcgc acctatcaga tggatcccgc
     2401 gaacggagac gaggcgctgc gcgaagtggc gctcgatctc gccgagggcg ccgacatggt
     2461 gatggtgaag ccgggcatgc cgtatctcga cgtgctgcgc cgggtgaaag atgaattcgg
     2521 cgtaccgacc ttcgcctatc aggtgtccgg cgagtactcg atgctgaccg gagccatcga
     2581 acgcggctgg ttcgaccgcg accgcgtgat gatggaaagc ctgatggcgt tcaagcgcgc
     2641 cggcgcgagc ggcatcctca cctatttcgc gcgcgatgcc gcgcggcttc tgaaaaaagg
     2701 ttcgtccgca atttgacgct tcgctctcca acctcccgtt cgccggtgcg atttctgtcg
     2761 cggcgttatt ggcgcacccg tgtcgtgttc tggggcggcg cgatcacagt cggactcgtc
     2821 agcgtcgtct tcgcgaaggc ggcgaattac gcgcaagcac tgtttgcgca tgtcctcgtc
     2881 aatccatgga tcgcgctcgg gctgacgccc gccggattcg ttttttccgt ttggctcgca
     2941 cgcgccgtat ttccaggctc gcagggcagc ggcattccac aggccatcgc ggcgcgcgaa
     3001 ctcgatgaac cgagcgcgcg aaaaaaactt ctatcgctga aaatcgcctt cggcaaaatc
     3061 ctgctgacat tggtcggact ggcctccggc gcctccatcg gccgcgaagg gcccaccgtg
     3121 caggtcgggg cagcgatcat gctgttcacc gcgcgcgtcg gcgatgcagc gctggaacgc
     3181 gggctgatcc tcgccggatc
//
LOCUS       DASZMC010000038         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_566267,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000038 DASZMC010000000
VERSION     DASZMC010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566267"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VG274_00725"
     CDS             <1..652
                     /locus_tag="VG274_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWE05197.1"
                     /translation="WLKANDRPGIPLVIVHSHHHSDHVWGDSQFDGRPDTTFIKPDLD
                     HLKTFFGFTKWPEETRTFDLGDRPLELIPVPGHTDDSFALYDHRTGILLTGDTVYPGR
                     LYISDFAAFKQSIHRLAAFTNGKLISHVLGNHIEETATPYLEYPIGKMYQPEEHSLDL
                     SVGTILELDRALATMDAPRRLALRDLTIFPMDRQSSQELRGVSRATQQKDKAEVWK"
     gene            663..1055
                     /locus_tag="VG274_00730"
     CDS             663..1055
                     /locus_tag="VG274_00730"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HWE05198.1"
                     /translation="MTRATGKFEVKLTPQSQDKDPATGTALGRMSIEKQFHGDLEATS
                     RGEMLSAMTSVPGSAGYVAIERLTGNLHGRAGTFVLQHSGTMAHGHQELTIRITPDSG
                     TGELTGISGAMTIHISGGEHSYEFEYAL"
     gene            complement(1061..2020)
                     /locus_tag="VG274_00735"
     CDS             complement(1061..2020)
                     /locus_tag="VG274_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWE05199.1"
                     /translation="MNRYKLVHVTRFKYDGPVSESYNELRLRPRHDDAQSCLSFRITT
                     DPSSRPAAHLDYFGNWVHEFHILPEHRCLTVESEAVVLVHPPAPIMFLSLPLADIGHM
                     RDVLIDDHYDWLTESRFCPSLPALGDLARDVERASDLTARGFAESAATLIHERFRYQK
                     GATHVHSSISDCLATGAGVCQDFAHLLIALLRFQGIPARYVSGYLVPRHDNDPNTAME
                     RVIGGHASHAWVEAFIPEKGWIGLDPTIGSFLEAQHIRIAYGRDYGDVPPVRGVYKGH
                     AGQNLSVDVHVRPALDHEGSELLEETAAAPPAAAVSEDEQQQQ"
     gene            complement(2017..2964)
                     /locus_tag="VG274_00740"
     CDS             complement(2017..2964)
                     /locus_tag="VG274_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWE05200.1"
                     /translation="MMLSRIADSLFWIGRYMERADNTARILDVNYHMLLEQPPDTYKL
                     RWDPLITITGERTRFYQRYEEANAQTAFEHLGFSEENPNSISQCIVCARENARTIRDR
                     ISRELWEDINGLYHKLSKMRAGEESLTRFCDAVKRGGHRFHGVCDATLARDEGWHFLQ
                     TGMALERAEMTARILDVHYHRLLEGPQILNEAHSHAWVAVLKSVAAYEFYKRQYSQIE
                     PMRVSELLLLDGRHPRSVRFTLGALQNSLHAVSGSIPGTFANEGERLTGRAYERLTYD
                     SIEEIFNAGLHEFLTELQSTCRTIGEQITRTYFYYAVVA"
     gene            complement(2961..>3874)
                     /locus_tag="VG274_00745"
     CDS             complement(2961..>3874)
                     /locus_tag="VG274_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWE05201.1"
                     /translation="RLVMRRTFPRAFEVCEVLPVDHYPAALVQILRSLSPRQGERQNV
                     VLLTPGLYNSAYFEHSFLAQEMGIELVEGRDLVVVDDVVYMKTIHGLTRVDVIYRRID
                     DDFLDPLAFRSDSLVGVSGLMNAYRAGNIALANAVGTGVSDDKAIYAYVPEFIRYYLG
                     EDPVLENVHTWLCSDASHLSHVLDHLGELVVKAVGESGGYGMLIGPSASRQEIEAFRD
                     RLKADPRNYIAQPVIPLSRCPSFDASTGCMSPRHVDLRPYCLYDGDKVTIVPGGLTRV
                     ALKRGSLVVNSSQGGGSKDTWVLRGDE"
BASE COUNT          768 a         1045 c         1341 g          720 t
ORIGIN      
        1 ctggctgaaa gccaatgacc ggccgggtat cccgctcgtc atcgtgcatt cgcatcatca
       61 cagcgatcat gtgtggggcg attcgcagtt cgacgggcgg ccggacacga ccttcatcaa
      121 acccgacctc gaccacctga agacgttctt cggcttcacg aaatggccgg aagagacgcg
      181 cacgttcgat ctgggcgatc gtccgctcga gctgattccg gttcccggcc acacggacga
      241 ttccttcgcg ctttacgacc accggaccgg cattctgctg acgggggata cggtgtatcc
      301 ggggcggctt tatatcagcg acttcgccgc cttcaaacaa agcattcatc gccttgccgc
      361 gtttacgaac ggcaagctga tttcgcacgt gctgggcaat cacatcgaag aaactgcgac
      421 gccttatctg gaatacccga tcgggaaaat gtatcagccc gaggagcatt cactcgattt
      481 gagcgtgggg acgattctgg aactcgatcg ggcgctcgca accatggacg cgccgcggcg
      541 gctggccctg cgcgatctca cgatttttcc catggaccgg caatcctcgc aggagcttcg
      601 cggcgtaagc cgcgccacgc agcaaaagga caaagcggaa gtctggaagt aacctgaacg
      661 atgtgacgcg cgcaacaggg aaattcgagg tgaagctgac gcctcaatcg caggacaaag
      721 atccggcaac cgggacggct ctgggccgca tgtcgatcga gaagcaattc catggcgacc
      781 tggaggcgac cagcaggggc gaaatgctga gcgcgatgac gagtgttccg gggtcggccg
      841 ggtacgtcgc catcgagcgc ctaaccggca atctacatgg ccgcgcggga acatttgttc
      901 ttcagcacag cgggacgatg gcgcatgggc atcaggagtt gacgatcagg ataacgcccg
      961 attccggaac cggcgaactg acgggcattt ccggagccat gacgattcat atctccggcg
     1021 gcgaacactc gtacgaattc gagtacgcgc tttaatcccg ttattgttgt tgttgttcgt
     1081 cttcggatac ggcggcggcc ggaggagcgg cggcggtttc ttcgagcagc tccgagcctt
     1141 cgtgatcgag ggccggcctg acgtgaacgt ccacggagag gttctgtccg gcgtggcctt
     1201 tatacacgcc gcgcacggga gggacgtcgc cgtagtcgcg cccgtaggcg atgcggatgt
     1261 gctgcgcttc gagaaacgat ccgatggtag ggtcgagtcc gatccagccc ttttcgggga
     1321 tgaaggcctc cacccaggcg tgcgaggcgt gcccgccgat tacgcgttcc atggccgtgt
     1381 tgggatcgtt gtcgtggcgc ggcacaaggt atccggatac gtaacgcgcc gggattccct
     1441 ggaagcgcag cagcgcgatc aggagatggg cgaagtcctg gcagacgccc gcgccggtgg
     1501 cgaggcaatc ggagatggag gagtgaacat gcgtcgcgcc tttctgataa cggaagcgct
     1561 cgtgaatcag cgtggccgcc gattcggcga aacctcttgc ggtcaggtcc gaggcccttt
     1621 cgacgtcgcg cgccagatcg ccaagcgcgg gaagcgacgg acaaaatcgc gattcggtga
     1681 gccagtcgta atgatcgtcg atcaggacgt cgcgcatgtg cccgatatcg gccaggggca
     1741 gggaaaggaa catgatcggg gcggggggat ggacgagtac gacggcttcg gattcgacgg
     1801 tgaggcagcg gtgctcgggc aggatgtgga attcatgtac ccagttgccg aaatagtcga
     1861 gatgggcggc ggggcgtgag gacgggtcgg tggtgatgcg gaaggagagg cagctttggg
     1921 cgtcatcgtg gcgaggacgc aggcgaagct cgttataact ctcggagacc ggtccgtcgt
     1981 acttaaaccg ggtgacgtgg acaagtttgt atcggttcat gccactaccg cgtaatagaa
     2041 gtaagttcgc gtaatctgtt cgccaatcgt gcggcaggtg ctctgcagtt ccgtgaggaa
     2101 ttcgtgcagg ccggcgttga agatttcttc aatgctgtcg taagtgagcc gttcataggc
     2161 gcggccggtg agccgctcgc cctcattggc gaaggtgccc gggatggaac cgctgacggc
     2221 gtgcagcgaa ttctgcagcg ctccgagggt gaagcgaacc gagcggggat ggcggccatc
     2281 gagcagcaga agctcggaga cgcgcatggg ctcgatctgc gaatattgcc gcttgtagaa
     2341 ctcgtaggcc gcaacggatt tcagcacggc gacccaggcg tggctgtgcg cctcattgag
     2401 aatctgcggg ccttcgagca ggcggtggta atgcacgtcg aggatgcggg ccgtcatctc
     2461 ggcgcgttcg agcgccatac cggtttgcag aaaatgccag ccttcatcgc gcgcaagcgt
     2521 ggcgtcgcac acgccgtgga aacgatgtcc gccgcgtttg acggcgtcgc agaagcgggt
     2581 gaggctttct tccccggcgc gcattttcga gagcttgtga taaaggccgt tgatatcttc
     2641 ccagagttcc ctcgatatgc ggtcgcgaat ggtgcgggca ttttcgcgcg cgcaaacgat
     2701 gcattgggaa atggaattgg gattttcctc ggagaagccg agatgttcga aggcggtttg
     2761 ggcgttggcc tcttcatagc gctggtagaa gcgcgtgcgt tcgccggtga tggtgatgag
     2821 cgggtcccag cgcagcttgt aggtatcggg aggctgctcg agcagcatgt ggtaattgac
     2881 gtcgaggatg cgcgcggtat tgtcggcgcg ttccatgtag cggccgatcc agaacagcga
     2941 atcggcgatg cgggagagca tcattcgtcg cctcgcagga cccaggtgtc cttgctgccg
     3001 ccgccctgtg acgagttcac cacgagagag ccgcgcttga gggcgacgcg ggtgaggccg
     3061 ccggggacga tggtgacctt atcgccgtca tagagacagt aaggccggag atcgacatgg
     3121 cgcgggctca tgcatccggt ggaggcgtca aaggacgggc agcgggaaag cggaatgacg
     3181 ggctgggcga tgtaattgcg cgggtccgcc ttgagccgat cgcggaaggc ttcgatctcc
     3241 tggcgcgaag ccgaagggcc gatcagcata ccgtaaccgc cggactcgcc cacggccttg
     3301 actaccagtt cgccgagatg atcgagcacg tggctcagat gggaggcgtc cgagcaaagc
     3361 caggtgtgga cgttctcgag gacgggatct tcgccgaggt aatagcgaat gaactcgggc
     3421 acgtaagcgt agatggcctt atcgtccgaa acgccggtgc cgacggcgtt ggcgagcgcg
     3481 atattgccgg cgcggtaagc gttcatcagg ccggagacgc cgacgaggga atcggagcgg
     3541 aaggcgaggg ggtcgaggaa gtcgtcatcg atgcggcggt agatcacgtc gacgcgggtg
     3601 aggccgtgaa tggtcttcat gtagacgaca tcgtcgacga cgaccagatc gcggccttcg
     3661 acgagctcga ttcccatttc ctgggcgagg aagctgtgct cgaaataggc gctgttgtag
     3721 aggccggggg tgagcagaac cacgttctgg cgctcgccct ggcggggcga aaggctgcgc
     3781 agaatttgca cgagggccgc cgggtagtga tcgacgggaa ggacttcgca gacctcaaag
     3841 gcgcgcggga aggtgcggcg catgacgagg cggt
//
LOCUS       DASZMC010000039         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4415141,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000039 DASZMC010000000
VERSION     DASZMC010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4415141"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1913)
                     /locus_tag="VG274_00750"
     CDS             complement(9..1913)
                     /locus_tag="VG274_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05202.1"
                     /translation="MPLRIETFRNDVGGSAVYKAFSHPLAVPAAHALIEKISARGSVA
                     IYDPDGIAAAFDTFYPLAGTGIAHYFVQNIEHLTRNFRGVPAEPVTAVAAHRPQAILV
                     ASFDPAKTLAPIRHILPEQTEILSFEPLKLPVGMQTDRARYLVPLNFATNFVFFRDQA
                     DRHTRLVTANYWTRYGAIDVSLWCRLFGQDGRTLATWTEKCGGPESSLVIDSQTVRAR
                     FDLAAFAGQLFVHVVGAAGHDIVKYALDLYGDDGKTLSATHDANSWPADLYAGLPAPA
                     ADEDVVLWIQNSHPVAVPAGDIGLARMGANEVAWLNEPIAAFATHALSARNLLPDLSW
                     PAQIEIHAGKHVVRPRYEVTARNGRSRIAHPNVERSDLKTDPELARLGPLFGKGHVLP
                     AALLPPEVYTTIALPTPMSTVQRSLPVKALVYDAEGARVAEHRFGALARDHDSALDVT
                     MLAGSSLKTKFGHVELVYDFDAGDDADGWLHALFRYRNRSSGHAAETSFGSHMFNCAL
                     TYRGEPQSYSGPPPGLSTRLFLRVAPQPWNTFCHLIYPVSQTWHAYSQTVIILRSTGG
                     EERARVELKIPASGSRLWRVQDLFDEGLIARAGAHPYILVRDETCRLFGYHGVEGGEG
                     SFSLDHMFGF"
     gene            complement(1897..2550)
                     /locus_tag="VG274_00755"
     CDS             complement(1897..2550)
                     /locus_tag="VG274_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWE05203.1"
                     /translation="MDEQRQMLDYERLNTTEIATEPFPYAIVPGFVAADARDAIDGDY
                     PKITRSGSFPLSTLSYGPAFAQLIGELTGERMRALVAGKFGIDLEGRPVMATVRGQCT
                     ARDGHIHTDSDTKLVTMLLYTNRSWECASARLRLLRSTKRLDDYAAEVPAAESTLVIF
                     RNAPNAWHGFAPFHGQRRVIQVNWVSDPSVVAREEARHRFSAFFKRLVPDRASAVAH"
     gene            complement(2610..>3387)
                     /locus_tag="VG274_00760"
     CDS             complement(2610..>3387)
                     /locus_tag="VG274_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HWE05204.1"
                     /translation="GHALRLCMDKTFVWFGPAHIAAIALSVCAPLGLAAFGRGNPRLT
                     RAIRLVLAGELIATWILWYWMIVARGWVSTHTLLPMDLCSWASIAAIVTLLRPGQRSF
                     ELAYFWALTGTLQALITPELFYDFPDPRFIVFFAFHGGAIASVLFLVLGAGMRPYPAS
                     IPRVLAASLVYLVVTLATNRIFHTNFGYLSGKPAKPSLLDLMGPWPIYIVEVAALGVV
                     LVLMLYSPFYIADTMKNRGRLKPPAEGESGKKALHFPVRR"
BASE COUNT          686 a         1000 c         1119 g          582 t
ORIGIN      
        1 cgaccacgtc agaagccgaa catgtgatcc aggctaaagc tgccctcgcc gccttcgacg
       61 ccatgatagc cgaagagccg gcaggtttcg tcgcgcacga gaatataggg atgggcgccg
      121 gcacgcgcga tgaggccttc atcgaaaaga tcctgcacgc gccacagacg cgacccggat
      181 gcgggaatct tcaattcgac gcgcgccctc tcttcgccgc cagtcgaacg cagaataatg
      241 acggtctgcg agtatgcatg ccaggtctgc gacacgggat agatgagatg acagaacgtg
      301 ttccacggct gcggcgctac gcgcaggaag agacgcgtcg agagtcccgg cggcggaccg
      361 gaataggatt gcggctcgcc gcgataggtg agcgcgcagt tgaacatgtg cgagccgaag
      421 ctggtttcgg cggcgtggcc gctgctccgg ttgcggtagc ggaaaagcgc atgcagccag
      481 ccatccgcat cgtcgcctgc gtcgaaatcg taaaccagct ccacatggcc gaacttcgtc
      541 ttgagcgaac ttcctgcaag catggtcaca tcgagcgccg aatcgtgatc gcgcgccagc
      601 gcgccgaagc gatgctccgc cacgcgcgcg ccttccgcat cataaacgag cgccttgacg
      661 ggaaggctgc gttgcacggt ggacatgggc gtgggaagcg cgatggtggt gtagacctcg
      721 ggcggaagca gtgcggccgg aagcacgtgg cctttgccga agagaggacc aagtcgtgca
      781 agttctggat ccgttttcag gtcgctgcgc tcgacgtttg gatgggcgat gcgcgagcgg
      841 ccgttccgcg ccgtgacctc gtagcgcggc cgcacgacgt gcttgccggc atgaatttcg
      901 atctgtgcgg gccacgacag atccggcagc agatttcttg cactcaaagc gtgggtggca
      961 aaggcggcga tcggctcgtt cagccacgca acctcattgg cgcccatgcg cgcaaggccg
     1021 atatcgcccg ccgggacggc gaccgggtga ctgttctgga tccagaggac gacgtcctcg
     1081 tccgcagcag gcgcaggcag tccggcgtag agatcggccg gccaggaatt cgcatcatgc
     1141 gtggcggaca gcgttttccc gtcatcgcca taaagatcga gggcgtattt gacgatgtcg
     1201 tggcctgcgg cgcccacgac atgcacgaag agctggccgg cgaatgcggc cagatcgaag
     1261 cgcgcgcgca ccgtttggct gtcgatgacg agagaagatt cgggacctcc gcatttttcg
     1321 gtccatgtcg cgagcgtccg gccgtcctgg ccaaagagac ggcaccacag gctgacatcg
     1381 atggcgccat agcgcgtcca gtaattcgcg gtgacaagcc gggtgtggcg gtccgcctgg
     1441 tcgcgaaaga agacgaaatt ggtcgcaaaa ttgagcggca cgagatagcg cgcgcgatcg
     1501 gtctgcatcc cgaccggaag cttgagtggc tcgaagctca atatctctgt ctgctcgggg
     1561 agaatgtggc ggattggtgc gagcgttttt gcgggatcga aactcgcgac gagaattgcc
     1621 tgcggccgat gcgccgcaac cgccgtcacc ggttcggccg gcacgccgcg aaagtttctc
     1681 gtgagatgct cgatgttctg aacgaagtaa tgtgcaatcc cggtgccggc gagcggatag
     1741 aatgtatcga atgcggcggc aattccatcc ggatcgtaga tcgcaacgga gccacgggcg
     1801 gagatttttt caatcaatgc atgcgcagcg ggcacggcca ggggatgcga gaaggccttg
     1861 taaaccgcgc tgccgcccac atcgttgcga aacgtctcaa tgcgcaacgg cactggcgcg
     1921 atccgggacg agacgtttga aaaatgcact gaagcggtga cgggcttcct cgcgcgctac
     1981 aacgctcgga tcggacaccc aattcacctg gatgacgcgc cgttggccat ggaaaggcgc
     2041 aaatccatgc caggcgttcg gtgcgttgcg gaagatcacc aaggtgcttt ccgcagccgg
     2101 cacttcggcg gcgtaatcgt cgagccgctt cgtggagcgc aaaagcctca accgcgcgct
     2161 cgcgcattcc cagctgcggt tcgtgtagag cagcatggtg acgagcttcg tgtcgctgtc
     2221 ggtgtggatg tgtccgtctc ttgccgtgca ctggccgcga accgtcgcca tgaccggcct
     2281 tccttcgaga tcgatgccga attttccggc aacgagtgcg cgcatgcgct cgccggtaag
     2341 ttcgccgatc agctgcgcaa atgccggacc gtaggacagc gtcgacagcg gaaagctccc
     2401 ggagcgcgtg attttggggt agtcgccgtc gatcgcgtcg cgcgcgtcgg cagcgacaaa
     2461 acccgggacg atcgcgtagg ggaagggttc ggtggcgatc tcggttgtgt tcaggcgttc
     2521 gtaatccagc atctgccgct gttcgtccat gaagatatcg tccggccgcg aagccccgag
     2581 ttccggcggc cagcctggtc ccagacctat cagcggcgga ccggaaagtg tagggctttt
     2641 ttcccgcttt ccccttcagc cggcggcttg agccgtccgc gatttttcat cgtgtcggcg
     2701 atgtagaagg gcgaatagag catcagcacc agcacgacgc cgagtgccgc gacctccaca
     2761 atatagatgg gccacggccc catcaggtcg agaagcgagg gtttggcggg cttgccgctg
     2821 agatagccga aattcgtgtg gaaaatccgg ttcgtcgcaa gcgtcacgac gagatacacg
     2881 agagatgctg cgagcacgcg cgggatcgac gcggggtagg gccgcattcc ggcgccgagc
     2941 acgagaaaca gaaccgatgc gatcgcgccg ccgtgaaagg cgaaaaacac gatgaagcgg
     3001 ggatcgggaa aatcgtagaa caactcaggc gtgatgagcg cctgcaacgt tccggtcaac
     3061 gcccagaaat acgcaagctc gaagctgcgc tggccgggcc ggagcagcgt gacgatggca
     3121 gcgatcgacg cccagctgca cagatccatg ggaagcagcg tgtgcgtgga cacccagccg
     3181 cgcgccacga tcatccagta ccagagaatc caggtcgcga tcagctctcc tgcaaggacc
     3241 aggcggatgg cccgcgtcag gcgcgggttc ccgcgcccga acgccgcaag cccgagaggc
     3301 gcgcagacgc tcagggcgat tgcggcgatg tgcgcagggc cgaaccagac gaatgtcttg
     3361 tccatgcaca atcgtaatgc atggccg
//
LOCUS       DASZMC010000040         4539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3863253,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000040 DASZMC010000000
VERSION     DASZMC010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3863253"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VG274_00765"
     CDS             complement(<1..549)
                     /locus_tag="VG274_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HWE05205.1"
                     /translation="MEDVVIVSAARTAVGSFNGAFANVPAHELGRTAIKAALERANID
                     GKDVSETIMGQILTAAQGQNPARQASMNAGIPFETPAWIVNQLCGSGLRAVALGYQAI
                     LNGDATVIVAGGQESMSQAPHAAYLRAGQKMGALEFTDTMLKDGLIDAFHGYHMGVTA
                     ENVAREWQITREMQDKFAVASQN"
     gene            792..1388
                     /gene="phaR"
                     /locus_tag="VG274_00770"
     CDS             792..1388
                     /gene="phaR"
                     /locus_tag="VG274_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HWE05206.1"
                     /translation="MEDKAKTDPVIIKKYANRRLYNTQTSTYVTLDHLCEMVKAGTEF
                     EVRDARTGEDITRSVLTQIIFEEEAKGQSLLPIKFLRQLIRFYGDSLQGFVPGYLDMS
                     MDSFTKNQEAMRTRIAEAFGGGSQVIENMTRQNLAMFERAMQMFSPFTLKTGRVADEE
                     PKANGTSETKAKPSEDISELKSEMEAMRRQLAELSQRK"
     gene            complement(1392..3098)
                     /locus_tag="VG274_00775"
     CDS             complement(1392..3098)
                     /locus_tag="VG274_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HWE05207.1"
                     /translation="MLVQSLQKNSADGVDALRAVDDAERLTLAVSGAGVVTFSWTVAD
                     DVIVWDGARDILPNQYGAENINSGRTLLSFANADGRAKLTSIINGKSVQPTQFDIDIE
                     FASAMGSVWFVMVGTRVPGANGATERMTGVMREITERRREKQRLTYLATRDELTGQLN
                     RNSLRTELAQAIDHAKAEERNCAFLVASIDRLAMINDTYGFDAADEVIVAVGERLARM
                     LRSTDVIGRTAGNKFGVILGNCSERELALVADRLRAAVRDRIIDTRSGMVSATCSAGA
                     VLLPRGASSSQEAMLRAEETLTHARSAGRDGFAVYERSPQLETGRLRLMGIADEVVAA
                     LKDDRLLFAYQPIIDAKTRLVTHHECLLRMRQEDGTIATAGYFIPAAEQLGLVRLVDR
                     RALEMTVAKLHEQPNVVLGVNVSGTTAGDPSWLKSFIDYVHANASVAKRMIVELTETA
                     ALHHFEENANFVSQLRELGCRVAIDDFGAGYTSFRNLQMLHVDMVKIDGSYVQGLATS
                     PDNQIFVRTLVDLAKNFKLKTVAEWVGSAEDAALLESFGIDYFQGFHFGQPVLDPEWA
                     KA"
     gene            complement(3514..4116)
                     /locus_tag="VG274_00780"
     CDS             complement(3514..4116)
                     /locus_tag="VG274_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05208.1"
                     /translation="MTALAVMLLAASIAGWIAVTDGPPAARAYVRFACVLYAALAAAS
                     AIGAQLAGAVTLIVSACAPVLLAIAHRIVFRAPVAASIAASVLVVGCIAGMTAAATNI
                     AVLSFAPLSVSVLVIIANSLRDFSDRRGPAIQAIASAAALLAGASAFAAGGEAAQPAL
                     ILFSAAGLLGIALALAPRSDAAIEQKGAPDLHAGTIRPAR"
     gene            complement(4113..4427)
                     /locus_tag="VG274_00785"
     CDS             complement(4113..4427)
                     /locus_tag="VG274_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE05209.1"
                     /translation="MALFFDSEWFDARLATAGLKRSDLAAALGLSEHEIRELWKDQRE
                     ITADNVRVIAALLGASPYDVANRAGVSTAVPQDNHDDRLERIELELREIKTLLVELRA
                     KR"
BASE COUNT          951 a         1435 c         1346 g          807 t
ORIGIN      
        1 gttctgcgac gcgaccgcga atttgtcctg catctcgcgc gtgatctgcc attcgcgtgc
       61 gacgttctcg gcggtgacgc ccatgtgata gccgtggaac gcgtcgatca gaccgtcctt
      121 cagcatcgtg tcggtgaatt cgagcgcacc catcttttga ccggcacgaa gatacgcggc
      181 atgcggcgcc tggctcatcg attcctggcc gcctgccacg atcaccgtcg cgtcgccgtt
      241 caggatcgcc tgatagccga gcgcgactgc gcgcaggccg gagccgcaaa gctggttcac
      301 gatccacgcc ggagtctcga acggaatgcc cgcgttcatc gacgcctgac gcgccgggtt
      361 ctggccctgc gccgccgtca ggatctggcc catgatggtc tcggacacgt ccttgccgtc
      421 gatattcgcg cgttcgagcg cggccttgat cgcagtccgt ccgagttcgt gcgcaggcac
      481 attggcgaac gcgccgttga aggagccaac cgcggtgcgg gcggcggata cgatgacgac
      541 gtcttccatg agggacctct tcgcaggcgc gatgttgctg tcgcgttcaa cctgccgcga
      601 atgcgagcaa cggtaaacgg gttaaattta ccaagcaatt tcaaatacct aattcccaca
      661 cgcactatgg gcctttgctg caatcgcgta aaaacactgg acagggtgga aaatgtgatg
      721 cttttgttag gtatcgaagg cgcccgccaa gcggcgtgaa acacttatca attcaaatgg
      781 ttgggtgcgc agtggaagac aaagccaaaa ccgatccggt gatcatcaag aagtacgcga
      841 accggcggct ctacaacaca cagacctcga cctacgtgac gctcgatcat ctctgcgaga
      901 tggtgaaggc gggcacggag ttcgaagtgc gcgacgcccg cactggcgag gatattaccc
      961 gctcggtgct gacccagatc atcttcgagg aagaggccaa gggccaaagc ctgttgccga
     1021 tcaaattcct gcgccagctc atccgcttct atggcgacag tctgcagggc ttcgtgccgg
     1081 gctatctcga tatgtcgatg gacagcttca ccaagaacca ggaagcgatg cgcacacgca
     1141 tagccgaagc gttcggcggc ggcagccagg tcatcgaaaa catgacgcgt cagaacctcg
     1201 ccatgttcga gcgcgcgatg cagatgtttt cgcccttcac gttgaagaca ggacgcgtgg
     1261 ccgacgaaga gccgaaggca aacggcacca gcgaaacaaa agccaagcca tcggaagaca
     1321 tcagcgaact caagagtgag atggaagcca tgcgccgcca gctcgcggag ctcagccaac
     1381 ggaaataagc gttacgcttt ggcccattcc ggatccagca ctggctggcc gaagtggaag
     1441 ccctggaagt aatcgattcc gaagctttcc agaagcgccg catcttctgc cgaaccaacc
     1501 cattccgcga cggtcttgag cttgaagttc ttcgcgaggt cgacgagcgt gcggacgaag
     1561 atctgattgt cgggcgatgt cgcgagccct tgcacataag agccatcgat cttgaccata
     1621 tcgacgtgca gcatctgaag attgcggaaa gacgtgtagc cggcgccgaa atcgtcgatg
     1681 gcgacgcggc agccgagttc gcgcaactgg ctcacgaaat tggcgttctc ctcgaaatga
     1741 tgcagcgccg ccgtctcggt aagctcgacg atcatccgct tggcgacgga cgcgttggca
     1801 tgcacgtagt cgatgaacga tttcagccat gacggatcac ctgccgttgt acccgagaca
     1861 ttcacgccca gaacgacgtt cggctgctca tgcaacttgg cgacagtcat ctccaatgca
     1921 cggcggtcga cgaggcgcac gaggccgagc tgctccgcgg cgggaatgaa gtacccggcc
     1981 gtggcaatcg tcccgtcttc ctgccgcatg cgcagcaggc attcgtgatg cgtcacgagg
     2041 cgcgtcttgg catcgatgat cggctgatag gcaaaaagca ggcgatcgtc tttcagcgcc
     2101 gcgacgactt cgtccgcgat tcccatgagg cgcagacggc cggtttcgag ttgcggcgac
     2161 cgctcgtaca ccgcgaagcc atcgcgcccg gcgctgcgcg cgtgtgtcaa cgtctcctcg
     2221 gcgcgcagca tcgcttcctg gcttgaggat gcgcctcgcg gcagaagaac ggcaccggcc
     2281 gagcatgtcg ccgacaccat gccggagcgc gtatcgatga tgcgatcacg cacggccgcc
     2341 cgcaggcgat cggcgacgag cgccagttca cgctcgctgc aattgccgag aatgacgccg
     2401 aacttgttgc ccgccgtgcg gccaatgaca tcggtagagc gcaacatgcg cgccagacgc
     2461 tcgcccaccg cgacgattac ctcgtccgcg gcgtcgaagc cataggtgtc gttgatcatc
     2521 gcgaggcggt cgatggacgc cacgaggaat gcgcagttcc gctcctcggc cttcgcgtgg
     2581 tcgattgcct gcgccagctc ggtgcgcagc gagttgcgat tgagctggcc cgtgagttcg
     2641 tcgcgcgtgg cgagataggt caagcgctgc ttctcacggc gccgctcggt gatctcgcgc
     2701 atcacgccgg tcatccgctc ggtcgcaccg ttggcgcccg gaacgcgcgt accgaccatc
     2761 acgaaccaga ccgaccccat cgccgacgcg aattcgatat cgatatcgaa ctgcgttggc
     2821 tggacggatt ttccgttgat gatcgatgtg agcttggcgc ggccatcggc gttcgcgaaa
     2881 ctcaacagcg tgcgaccgct attgatgttc tccgcgccgt attgattggg aagaatatcg
     2941 cgcgcgccat cccacacgat gacatcgtcg gcgactgtcc acgaaaacgt cacaacgcct
     3001 gcgccggaga cggcgagcgt caggcgttcg gcgtcatcga ccgcgcgcaa cgcgtcgacg
     3061 ccatcggcag agttcttctg cagcgactga accagcactt ccaccatccc ttggccgttt
     3121 tccggccttt ggaaggatga tgactgtcgc gacttaaaaa actcgaaact tttaggacaa
     3181 agcgcggaac gaacctgcgc ggcgcgcact ttgccttaag aacccgttat gagcatacac
     3241 gcacctcgcg gggctgggtc agacaaggac gcatggaacg cgccaattcg cgggctgcgc
     3301 ctggaaaagc cgcgcgaaac cagcgcgcta catgatgaag atcgcggcaa ccaaaaaaat
     3361 ctagatcggc gcggccaaaa atccacgcgc cgtgcagcgt atcgttttga tacaacgggc
     3421 gacctgtcgc actggaatgg accactcctg acggcgccgt tcgccgcaca agtcatcggc
     3481 caagctttgg acgcccacga agacagcgcg acctcagcgc gccgggcgta tcgtcccggc
     3541 atgcagatcg ggcgcgcctt tttgttcgat agcagcatct gaacgcggcg ccagcgctag
     3601 cgcgatcccc agcaacccgg ccgctgaaaa caggatcagc gcgggctgcg ccgcctcgcc
     3661 accggcggca aacgccgatg cgcccgcaag tagcgcagct gcggaagcaa tcgcctgaat
     3721 tgccggcccg cgtctatcgc tgaaatcgcg aagactgttt gcaatgatca caagcacgga
     3781 gaccgacaac ggcgcaaagc tcagaacagc gatgttggtc gccgcggccg tcatgccagc
     3841 gatgcaacca accacgagta cagatgcggc aatcgacgct gcgacgggtg cgcgaaacac
     3901 aatccgatgt gcgattgcca gcaacacagg cgcgcaagcc gacacaatga gcgtgaccgc
     3961 ccccgcgagt tgcgcaccaa tcgcgctggc tgcagccagt gcggcataga gcacgcaagc
     4021 aaaccgcaca taggctcgag cggccggcgg cccatcggtg acggcaatcc aacccgcgat
     4081 agacgcagca agaagcatga cggcgagcgc tgtcatcgtt ttgcccgcaa ttccacgagc
     4141 aacgtcttga tctccctgag ttccaattcg atccgctcca gccggtcgtc gtgattgtcc
     4201 tgcggcaccg ccgtcgacac gcccgcgcgg tttgcgacgt catatggcga tgcgccaagc
     4261 agtgctgcga tgacgcgcac attatcggca gtgatttcac gctgatcttt ccagagctcg
     4321 cggatttcgt gttcgctcaa accaagcgcc gccgcgagat cgctccgctt cagccccgcc
     4381 gttgcgaggc gtgcgtcaaa ccattccgaa tcgaagaata gcgccatggc tgcagagatt
     4441 atgcgccccg cgcctgcgac tcgctacagt tcgggcgcga aagccggatg gttgagcatt
     4501 gctgcaccca tcaccttgcc ataaccgccc ccccccctc
//
LOCUS       DASZMC010000041         8712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3047060,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000041 DASZMC010000000
VERSION     DASZMC010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3047060"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..525
                     /locus_tag="VG274_00790"
     CDS             112..525
                     /locus_tag="VG274_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05210.1"
                     /translation="MLAASLVAACSGSPKPRLQGEQWHPPTEMLDKYANQNGVLTRAA
                     LEAGLRADFVAADVGHTGCLDENKVREINEARWKEGASTSSPLIDFRHNGCIDFEEFS
                     ATPRSLFEQLDKGGKGRLTPKELHPERKPQSSSAQ"
     gene            complement(571..1359)
                     /locus_tag="VG274_00795"
     CDS             complement(571..1359)
                     /locus_tag="VG274_00795"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HWE05211.1"
                     /translation="MSIFDFNRAIVREPGGSVVNGIRTDPNVVVDYEGILGEHRAYVG
                     ALRAAGLKVEVLPPLQQYPDSIFVEDPALVLPEGAILLRPGAPGRRGESGDMRGVLKR
                     HFQQVFELDGDEFADGGDVLITPAGVLIGTSRRTNRAGANALRAKLDRFGRKSRIVET
                     PEGILHLKTAASLLDENVVLATARVADSGIFADFRILVVPLDEAAAANALRINDTVLV
                     GACFPRTAELVAREGFRVQTLPVTETGKLDAGLSCMSLRWHAQS"
     gene            1703..1779
                     /locus_tag="VG274_00800"
     tRNA            1703..1779
                     /locus_tag="VG274_00800"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1737..1739,aa:Asp,seq:gtc)
     gene            1912..2277
                     /locus_tag="VG274_00805"
     CDS             1912..2277
                     /locus_tag="VG274_00805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWE05212.1"
                     /translation="MQSLLLEYLPILLFTAIAGGIGAALIAVPFLIAPQAPDTEKLSA
                     YECGFNAFGDARMKFDVRFYLVSILFIIFDLEVAFLFPWAITLRATGAFAFWSMMVFL
                     GVLTVGFIYEWKKGALEWE"
     gene            2277..2810
                     /locus_tag="VG274_00810"
     CDS             2277..2810
                     /locus_tag="VG274_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HWE05213.1"
                     /translation="MAPAGPIGIRAPGNDPYLGALKAEFDNKGFLVASADSLINWART
                     GSLMWMTFGLACCAVEMMQANMPRYDLERFGFAPRGSPRQSDVMIVAGTLCNKMAPAL
                     RKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALVY
                     GIMLLQRKIRRTGTIER"
     gene            2818..3426
                     /locus_tag="VG274_00815"
     CDS             2818..3426
                     /locus_tag="VG274_00815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004733.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWE05214.1"
                     /translation="METLEDLGRKIAAALPGAVPGRSIAFGELTLAIEPAEIVHALTY
                     LRDDPECRFTLLIDITGVDWPQRERRFDVVYHLLSLAGNRRIRLKLEIGENASVPSAV
                     GVFPAANWFERETFDMFGITFADHPDLRRLLTDYGFSGYPLRKDFPLTGYVELRYDEE
                     QKRVVYQPVDLVQEFRDFDFMSPWEGARYVLPGDEKASKEKK"
     gene            3423..4607
                     /locus_tag="VG274_00820"
     CDS             3423..4607
                     /locus_tag="VG274_00820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWE05215.1"
                     /translation="MMAESLDDRRMTLNWGPQHPAAHGVLRLVMELDGEIVTRLDPHI
                     GLLHRGTEKLIEHKTYLQALPYFDRLDYVAPMNQEHAFSLAIERLLGIEVPKRAQYIR
                     VLFCEIGRLLNHLLQITTQALDVGALTPPLWGFEEREKLMIFFERASGARLHANYFRT
                     GGVHQDLPAGLVEDIYAFCESFPKVVDDIDELLTENRIFKQRNVDIGAFTRKDAEARG
                     FTGVMLRSTGLAWDLRRAQPYECYSELDFRIPVGKNGDCYDRYLMRMEEMRQSTWLMK
                     QCIEKMPAGPVLSDDRKVVPPKRGEMKRSMEALIHHFKLFTEGYHVPAGEVYAAVEAP
                     KGEFGVYLVSDGSNKPYRCKIRAPSYPHLQAMEFLCKGHMLADVSAVLGSIDVVFGEI
                     DR"
     gene            4604..5212
                     /gene="nuoE"
                     /locus_tag="VG274_00825"
     CDS             4604..5212
                     /gene="nuoE"
                     /locus_tag="VG274_00825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005724.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HWE05216.1"
                     /translation="MSLRRLHHQQPSAFAFRPENKSWAEKEILKYPPGRQASAVIALL
                     WRGQEQEGWVSRPMIEEVARFLGMPYIRVLEVATFYTMFNLDPVGRHLVQVCTTTPCW
                     LAGSDDVVAACKKHIAPGPDTISADGVFSWTEVECLGACVNAPMMQIGKDFYEDLDGA
                     RTEEIFAALRRGETPQPGPQNGRHSSEPIGGATTLRNVPRHE"
     gene            5478..6767
                     /gene="nuoF"
                     /locus_tag="VG274_00830"
     CDS             5478..6767
                     /gene="nuoF"
                     /locus_tag="VG274_00830"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWE05217.1"
                     /translation="MLSDRDRVFTNLYGFEDWTLEGARARGQWDSTKAILEKGPDAII
                     KIMKDSGLRGRGGAGFPTGLKWSFMPKEVKDRPHYLVINADESEPGTCKDRDIMRHDP
                     HLLVEGCLIASFAMHAHICYIYVRGEFVRERERLQAAIDQAYEARLIGKNNIHGWDFD
                     CYVHHGAGAYICGEETALLESLEGKKGQPRLKPPFPANVGVYGCPTTVNNVESIAVVP
                     EILRRGADWFAGIGRPNNTGTKIFCISGHVNKPCNVEEAMSIPLRELIERHAGGVRGG
                     WDNLMAVIPGGSSMPVLPKETADTILMDFDSLVAVKSGLGTAAIVVMDKSTDIIKAFA
                     RLAYFYKHESCGQCTPCREGCGWMWRVLTRMQRGDAETSEIDLLQGVTKQIEGHTICA
                     FGEGASWPVQGFLRHFRPEIERRIAARADIIAAQAAE"
     gene            6772..>8712
                     /gene="nuoG"
                     /locus_tag="VG274_00835"
     CDS             6772..>8712
                     /gene="nuoG"
                     /locus_tag="VG274_00835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWE05218.1"
                     /translation="MAPRKLVIDGREIEAEDNITLLQACEQAGAEIPRFCYHERLSVA
                     GNCRMCLVEWVGAPKPQASCALQVRDIFPNKDGTPARINTNSPLVKKAREGVMEFLLI
                     NHPLDCPICDQGGECDLQDQAMAYGHASFHRFNENKRAVDDKYMGPLIKTIMTRCIQC
                     TRCVRFATEVAGVPDLGATGRGEDMEITTYLEKAFASELSGNAVDLCPVGALTSKPYA
                     FNARPWELRKTESIDVMDAQGASIRIDTRGGEVFRVLPRLNEEVNEEWISDKTRHACD
                     GLRQQRVDRPYVRKAGRLVPASWNEAFAAIAARVAATKSGRMAALVGDLAAAEEIKAL
                     KDLMISLGVANLDCRQDGARLQGSPRNSWLFNTTVAGVEAADAVLLIGTNPRWEAPVL
                     NARLRKTWLAGHVRFANVGQAYDLTYPVEQLGTKADVLEALASGNHPFAGVLRDAKRP
                     MLILGPGALARDDGAAILRLAAQIAGETGMIGPAEKRAQGGWNGFNVLHVAASRVAAL
                     DLGFLPGTGGRDTFGILDGATKGDIDFVYLLGADEIDMGALGNAFVVYQGTHGDAGAH
                     RADVVLPGAAYTEKEGLYVNFEGRVQRGRRAAFPPGEAKEDWAILRALSDAMGRRLPY
                     DDRETLRRAIVGDAPHFADIGSL"
BASE COUNT         1672 a         2684 c         2719 g         1637 t
ORIGIN      
        1 tttgactcgg caagctcccg gcaacttcgc ggcgacagaa gtcttgacca ctctccagcg
       61 tgtcggcgga atcgttgcat gcaaatagga ttacgtcatt ttctgaccgc gatgttggca
      121 gcatcgctgg ttgccgcttg ctccggttca ccgaagccgc gcctgcaggg tgagcaatgg
      181 catccgccga cggagatgct ggacaaatat gcaaatcaga atggcgtgtt gacacgcgcc
      241 gccttggagg cagggctgcg ggccgatttc gtcgcagccg acgttggtca tacgggctgt
      301 ctcgacgaga acaaggtgcg cgagatcaac gaggcgcgct ggaaggaagg cgcgtcgaca
      361 tcttcgccgc tgatcgattt ccgccacaat ggctgcatcg atttcgaaga gttctccgcg
      421 actccgcgct cgctgttcga gcagctcgac aagggcggca agggccggct cacgccgaag
      481 gaacttcatc ccgaacgcaa accgcaaagc tccagcgcac agtgacggcc gccgacgtag
      541 ttggcctgcg cgccgcgaat tccggcctgg tcaggattgc gcgtgccagc gaagcgacat
      601 gcaggaaagg cccgcatcga gctttccagt ctcggttacc ggaagtgttt gaacccggaa
      661 accttctctg gcgacaagtt ccgccgtacg cggaaagcac gcgcccacga gcacggtatc
      721 gttgatcctg agtgcattgg ctgccgccgc ttcgtcgaga ggaaccacga gaatcctgaa
      781 gtccgcaaat attccggaat ccgccacgcg cgccgtcgca agtacgacgt tttcatccag
      841 tagcgaagca gccgtcttga gatgcaggat accctcgggt gtttcgacga tccgggactt
      901 tcggccaaag cgatcgagct tcgcgcgaag tgcgtttgcg cccgcgcggt tcgtgcgacg
      961 ggaggttcca atcagaacgc ccgcgggagt gatcagaacg tcgccgccat cggcaaactc
     1021 atccccatcc agctcgaaca cttgttgaaa atgacgcttg aggaccccgc gcatatcccc
     1081 gctttcaccc cgccggcccg gcgcgcccgg acgcaacagg atcgcgcctt ccggcagaac
     1141 aagcgctgga tcctctacaa agatggagtc cggatattgc tggagcggtg gaagcacctc
     1201 gaccttgagg cctgctgctc gcaatgcgcc aacataggcg cggtgttcgc caagaatgcc
     1261 ttcgtagtcc accacgacgt tggggtcggt gcggatacca ttgacgacgc tgccgccggg
     1321 ttcgcgcaca atcgcccggt tgaaatcgaa gatgctcata gactctgccc taattccgtg
     1381 cggaaactcg caatttgata ttcgagagtt acagtgtgta gcgttcatct tgaatccaac
     1441 atgtcatccc ggccaaagct tggacccaag gcaagcgccg cctcgtccct tgccctgttg
     1501 gattccctca cctgcgggac tcacaccaat agggtttgtg attcaactcg aacggccagt
     1561 agctcggtgc aaggcgcggc tgcgctttgc tcatttcctt ctacggatac attgacgcgt
     1621 gacacaattg cctatccttg acacttcgtc ccccggagcc gtacatcggc ccgccttcgc
     1681 ggagcccgcg aaggacgccc gcgggggcgt agctcagttg gttagagcgt cggcctgtca
     1741 cgccgaaggt cgcgggttcg agccccgtcg ctcccgccag ttcccgccgg aactctccgg
     1801 aaaacactct ttgtcgcagg gtggaatctt ccgctaaacc ccgcgttgca tgggggtgtg
     1861 cggcgtccta taccgcgcac gcgaaaaggg tgcgggggcc aggaacacgg catgcaaagc
     1921 ctgttgctcg aatatttgcc gatcctgctc ttcaccgcca tcgcgggcgg tatcggcgcg
     1981 gcgctgatcg cggttccctt cctcatcgcc ccgcaggcgc cggataccga gaagctgtcg
     2041 gcctatgaat gcggcttcaa cgccttcggc gacgcgcgca tgaagttcga cgtccgcttc
     2101 tacctcgtct ccatcctgtt catcatcttc gatctggagg tcgctttcct gttcccctgg
     2161 gcgatcacgc tgagggccac gggcgcgttc gccttctggt cgatgatggt gttcctgggc
     2221 gtcctgacgg tcggcttcat ctacgaatgg aagaaaggcg cgctcgaatg ggaatagtgg
     2281 cgcccgctgg cccaatcggt atccgcgcac cgggcaacga tccctatctc ggcgcgttga
     2341 aggccgagtt cgacaacaag ggattcctgg tggcgagcgc agactcgctg atcaactggg
     2401 cgcgcaccgg ttcgctgatg tggatgacat tcgggctggc atgctgcgcg gttgaaatga
     2461 tgcaggccaa catgccgcgc tacgatctcg agcggttcgg ctttgcgccc cgcggcagcc
     2521 cgcgccagtc cgacgtcatg atcgtggctg gcacgctctg caacaagatg gcgccggcgt
     2581 tgcgcaaggt gtacgaccag atgcccgagc cccgctacgt gatttcgatg ggctcctgcg
     2641 ccaatggcgg cggctactac cattactcct attccgtcgt gcgcggttgc gaccggatag
     2701 ttcccgtcga catttacgtt ccgggctgcc cgccaacggc cgaagcgctc gtctacggca
     2761 tcatgctcct gcagcgcaaa atccgccgga ccggcacgat cgagcgctga aagcaccatg
     2821 gagaccctcg aagatctcgg caggaagatc gccgccgcgc tgcccggcgc ggtgcctggc
     2881 cggtcgatcg cattcggcga attgacgctc gcaatcgagc ctgccgaaat tgtccacgca
     2941 ctcacctact tgcgcgacga ccccgaatgc cgcttcacgc tgctcatcga cattacgggc
     3001 gtcgattggc cccagcgcga gagacgcttc gacgttgtct atcatctgct gtctctcgcc
     3061 gggaaccggc gcatccgtct caaactcgaa atcggcgaga acgcttccgt gccctccgcg
     3121 gtgggcgtct ttcctgctgc gaactggttc gagcgcgaaa ccttcgacat gttcgggatc
     3181 acattcgctg atcatccaga tctgaggcgt ctgctgacgg actacggctt ttccggctac
     3241 ccgttgcgca aggactttcc tctgaccggc tatgtcgaat tgcgctacga cgaggagcaa
     3301 aagcgcgttg tctaccagcc cgtcgatctc gtccaggagt tccgcgattt cgatttcatg
     3361 agtccctggg agggcgcacg ctacgtgctg ccgggggatg aaaaagcctc gaaggaaaaa
     3421 aaatgatggc ggaatcgctc gacgaccgcc gcatgacgct caactggggc ccacagcatc
     3481 ccgcggcgca cggcgtgctg cgcctcgtca tggagctcga cggcgagatc gtcacccgcc
     3541 tcgatccgca tatcgggctc ttgcaccgcg gcaccgaaaa gctgatcgaa cacaagacct
     3601 atctgcaggc gctgccatat ttcgaccgcc tcgactatgt ggcgccgatg aatcaggaac
     3661 acgccttctc gctggcgatc gagcggcttc tgggaatcga ggtgcccaaa cgcgcccagt
     3721 acatccgggt gctgttctgc gagatcggcc gcctcctcaa tcaccttttg cagatcacca
     3781 cccaggcgct cgatgtcggc gcgctgaccc cgccgctttg gggtttcgaa gagcgcgaga
     3841 agctgatgat cttcttcgag cgcgcctcgg gcgcgcgcct gcatgcgaat tatttccgca
     3901 ccggcggcgt gcaccaggat ctgccggccg gactcgtgga ggacatttac gccttctgcg
     3961 agagcttccc caaggtcgtg gacgacattg acgagctgct aaccgagaac cgcatcttca
     4021 agcaacgcaa tgtcgatatc ggcgccttca cgcgaaagga tgcggaagcc cggggattta
     4081 ccggcgtcat gctgcgctcg accgggctgg cctgggatct gcgccgggcc cagccttacg
     4141 agtgctattc cgaactcgat ttcaggattc ccgtgggcaa gaacggcgac tgttacgacc
     4201 gctacctgat gcgcatggaa gagatgcgcc aatcgacctg gctgatgaag caatgcatcg
     4261 agaagatgcc ggccggcccg gtgctctccg acgatcgcaa ggtcgtccct ccgaagcgcg
     4321 gtgagatgaa gcgctcaatg gaagcgctca tccatcattt taagctgttc actgaaggct
     4381 atcacgtgcc ggccggcgag gtttacgccg ccgtcgaggc gccgaagggc gagttcggcg
     4441 tgtatcttgt ttcggacggc agcaacaaac cgtatcgctg caagatcagg gcaccgagct
     4501 atccgcatct gcaggcgatg gaattcctct gcaagggcca catgctggcc gacgtttccg
     4561 ccgtactcgg ctcgatcgat gtcgtgttcg gggagatcga ccggtgagtt tgcggcgcct
     4621 ccatcaccag caaccctcag cttttgcctt cagacccgag aacaagtcat gggcagaaaa
     4681 ggaaattctg aaatacccgc cgggccgcca ggcatcggct gtgatcgcgc ttctgtggcg
     4741 cgggcaggaa caggaagggt gggtctccag gccgatgatc gaggaggtcg cgcgcttcct
     4801 cggcatgccg tatatccgcg tcctcgaagt cgcgaccttc tataccatgt tcaatctcga
     4861 cccggtcggg cgccatctcg tccaggtgtg cacgacgacg ccctgctggc tggccggatc
     4921 ggacgatgtt gtcgccgcct gcaagaagca tatcgcaccc ggaccggata cgatttcggc
     4981 cgacggtgtg ttcagctgga cggaagtcga gtgcctgggc gcctgcgtca acgcaccgat
     5041 gatgcagatc ggcaaggatt tctatgaaga tctcgacggt gcaaggacgg aagagatctt
     5101 cgcggccttg cggcggggtg aaacaccgca gcccggtccg cagaacggtc gtcattcatc
     5161 cgaaccgatc ggtggcgcaa cgacgctcag gaatgttccg cgccatgagt gaatcggagc
     5221 aataaagttg aaacgccaag acgccaacat gccaaacctt ctcattgtca tggcccgcgc
     5281 aagcgggcca cccagtcgga gcctgcgaaa gcttgtctta atgcgtgcga gttttcgtcg
     5341 tctagttgac cgctcgtcgc ttccatctgg gtggcccgct tgcgcgggcc atgacaatga
     5401 aggagcgcat ccgggcacga atcttcttgg cgtcttggtg tcttggcggg tttcgtcgca
     5461 accgcgtgcg gtctgccatg ctttctgacc gggaccgtgt cttcaccaat ctctacggct
     5521 ttgaggattg gacgcttgaa ggcgcgcgcg cgcgcggcca gtgggacagc accaaggcca
     5581 ttctcgagaa aggccccgac gcgatcatca agatcatgaa agactccggc ctgcgcggcc
     5641 gcggcggggc gggatttccg accggattga aatggtcgtt catgccgaag gaagtgaaag
     5701 atcggccgca ctacctcgtc atcaatgccg acgaatcgga accgggcacg tgcaaggacc
     5761 gtgacatcat gcggcacgat ccgcatcttc tcgtcgaagg ctgcctcatc gcgtccttcg
     5821 cgatgcacgc gcatatctgc tacatctatg tccgcggcga attcgtccgc gaacgcgagc
     5881 gcctacaggc ggcgatcgac caggcgtatg aagccaggct gatcggcaag aacaacatcc
     5941 atggctggga tttcgactgc tacgtccacc atggcgccgg cgcctatatc tgcggagaag
     6001 agacggcgct gctcgaaagc ctggagggaa agaaggggca gccgcggctg aagccgccgt
     6061 tcccggccaa tgtcggcgtc tatggatgcc cgacgaccgt caacaatgtc gaatcgatcg
     6121 cggtcgtacc ggaaatcctg cggcgcggcg cggactggtt cgccggcatc ggccggccca
     6181 acaacacagg tacgaaaatc ttctgcatct cgggccacgt gaacaagccg tgcaatgtcg
     6241 aggaggcgat gagcattccg ctcagggaac tcatcgagcg ccatgccggc ggcgttcgcg
     6301 gaggctggga caatctcatg gcagtgatcc ccgggggctc atccatgccg gtgctgccca
     6361 aggaaaccgc cgatacaatc ctgatggatt tcgattccct ggtcgctgtg aagtctgggc
     6421 tcggcacggc cgccatcgtc gtgatggaca aatcgaccga catcatcaag gcgttcgcgc
     6481 ggctcgcgta cttctacaaa cacgagagct gcgggcaatg cacgccgtgc cgggaaggct
     6541 gcggctggat gtggcgcgtg ctgacgcgca tgcagagagg ggacgcggaa acatctgaaa
     6601 tcgatctcct gcagggagtc accaaacaga tcgagggtca tacaatttgc gccttcggcg
     6661 agggggcttc ctggccggtg cagggttttc tgcgtcattt ccgccccgag atcgagcgcc
     6721 gcatcgcggc acgggcggac atcattgcag cgcaggcggc ggagtagaga aatggcgccg
     6781 cgcaagctcg tcatcgacgg cagggaaatc gaggccgagg acaacatcac gctattgcag
     6841 gcatgcgagc aggcgggcgc ggagattccg cgcttctgct atcacgaacg cctttcggtt
     6901 gccggcaatt gtcgcatgtg cctcgtcgaa tgggttgggg cgcccaaacc gcaggcgagc
     6961 tgcgcacttc aggtgcgcga tatcttcccc aacaaggacg ggacaccagc acgcatcaac
     7021 accaactcgc cgctcgtcaa gaaggcgcgc gaaggcgtga tggaattcct gctgatcaac
     7081 catccgcttg attgcccgat ttgcgaccag ggcggagaat gcgacctgca ggaccaggcg
     7141 atggcctacg gccacgcgtc cttccatcgc ttcaacgaga acaagcgcgc ggtggacgac
     7201 aaatatatgg gtccgctgat caagacgatc atgacccgct gcatccagtg cacgcgctgc
     7261 gtgcgcttcg cgacggaggt cgccggcgtt cccgatctcg gcgccaccgg acgcggcgag
     7321 gatatggaga tcaccaccta tctcgagaag gcgtttgcgt ctgagctgtc gggcaatgcg
     7381 gtggatcttt gtccggtggg tgcccttacg tcgaagccct acgccttcaa cgcgcggccc
     7441 tgggaattgc gcaagacgga atcgatcgac gtgatggacg cgcagggggc gagtatccgc
     7501 atcgacacgc ggggcggcga agtgttccgc gtgctgccgc gcctcaacga ggaagtgaac
     7561 gaggagtgga tatccgacaa gacgcgccac gcctgcgatg gtcttcggca gcagcgtgtt
     7621 gacaggcctt acgtgcgcaa ggccgggcgg ctcgtgccgg cgagctggaa tgaagccttc
     7681 gccgccattg cggcgcgtgt cgctgccacc aaatccgggc gcatggcggc actggtgggc
     7741 gacctcgcgg cggcggaaga gatcaaggcg ctgaaggatc taatgatctc gctcggcgtg
     7801 gcgaacctcg attgccggca ggatggcgcg aggctgcagg gaagcccccg caactcctgg
     7861 ctcttcaata cgacggtcgc gggcgtggaa gcggccgatg cggttctcct gatcggcacg
     7921 aacccgcgct gggaagcgcc cgtgttgaat gcgcgcctgc gcaagacatg gctggccggc
     7981 catgtgaggt tcgccaatgt cgggcaggcc tacgacctga cctatccggt ggagcaactc
     8041 ggcacaaagg cggacgtact cgaggcgctc gccagcggca accatccgtt tgccggggtg
     8101 ctcagagacg ccaagcggcc aatgctcatc cttggacccg gcgcactcgc tcgcgacgat
     8161 ggcgccgcga tcctgagact cgccgcccag attgcgggcg agacgggcat gattggaccg
     8221 gcggagaaac gtgcgcaggg cggctggaac ggtttcaacg tgctgcacgt tgcggcgtcg
     8281 cgtgtcgccg cgcttgatct gggattcctg ccgggcaccg gcggccgcga cacattcggg
     8341 attctcgatg gcgccacgaa gggcgatatc gatttcgtct atctgctcgg cgcggatgag
     8401 atcgacatgg gcgctctggg gaacgccttt gtggtgtatc agggaaccca tggcgatgcg
     8461 ggcgcgcatc gcgcggatgt cgtgctgcca ggcgccgcct ataccgagaa ggaaggcctc
     8521 tacgtcaact tcgagggccg cgttcagcgc gggcggcgcg cggcttttcc acctggcgaa
     8581 gcgaaggagg actgggcaat tctgcgcgcg ctttccgacg cgatgggaag gcggttgcct
     8641 tacgacgacc gcgaaacctt gcgccgcgca atcgtcgggg acgcaccgca tttcgcggac
     8701 atcggctcat tg
//
LOCUS       DASZMC010000042         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3864922,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000042 DASZMC010000000
VERSION     DASZMC010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3864922"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VG274_00840"
     CDS             complement(<1..870)
                     /locus_tag="VG274_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HWE05219.1"
                     /translation="MKIGFLLPLVASACVFAATDTPALLQQKCGSCHGTSSGMSGLKV
                     TSRDNLLKGGSRGPALVPGKSADSLLYKAVAHIGDVSMPPNGALTADEVGAIKTWIDG
                     GAQWTAGSAASASATWWAFRKPVRPAVPAGPEKNPIDAFIDRKLASAGLTKAPPADRL
                     TLLRRASYDLLGLPPAQDEIDRFLNDRSPDAWEHEIDRLLASPRYGEKWGRHWLDLAR
                     YGDTAGFEQDPYLLISWRYRDYVIKSFNNDKPYDRFIREQIAGDELYPTDPDARTGTG
                     FYRVGPNRDMLFKV"
     gene            complement(997..2241)
                     /locus_tag="VG274_00845"
     CDS             complement(997..2241)
                     /locus_tag="VG274_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWE05220.1"
                     /translation="MSRLLLAAVILPALLAAQTPHKLLVISIGGLDARFLNDPALKVK
                     APNLRRLMKEGASATVVGVVPSDTWPSHASLVTGVSPWQNGITAEGLPERPGDAFFSV
                     DAVKAPTLWSAAVNGRLKVATVYWPSTLGAHVDWNFPEYWETRRDNAVPLADVGAKSF
                     PAGLTDRVAQMFPAFDKQLWDDTSSADAAMYLLSAEQPDVLFVHMGDLDAEQRETTAV
                     SIYARDILENDDDLVGQIVRKAPAGTIVAIVSDHGFQSSFHVVRPRVQLRQAGVKGTV
                     EVRDGLIGTRDAGVAKELRKLAAETRKSGIAREVPMAEVRARAPSLGKWIAAFDTPQD
                     YVARDEERGSAVGTGPHAGVCDFWPTHVNYRSLLILNGTGIKPTKLGEVDMLQIAPTL
                     AEILGVKLPLAKSTSLWRSVSH"
     gene            complement(2307..>2963)
                     /locus_tag="VG274_00850"
     CDS             complement(2307..>2963)
                     /locus_tag="VG274_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05221.1"
                     /translation="MRKQVLLALPAAAALLFAVETVKTPINNDDIRVLDVVVQPHEPT
                     SMHEHKANRVMIYKNAGAQNFKREDGHTSVLNFKEGEVKWSPIEGKHIAEVATEQPVG
                     IVEIELKKPGAGKKVTTALDPLKVDPKHYKLEFENDQVRVFRVKIGPHEGTPMHEHQL
                     NRVMVYLTDAVVRVTSSDGKVDVTPHKAGDIAQGGAARHSEQNTTDKPVEVVVTEFKY
                     "
BASE COUNT          521 a          929 c          931 g          582 t
ORIGIN      
        1 gaccttgaac agcatgtcgc ggttcgggcc cacgcgatag aagccggtgc cggtgcgcgc
       61 atcgggatcc gtcggataaa gctcatcgcc cgcaatctgt tcacggataa aacggtcgta
      121 cggtttatcg ttgttgaaag acttgatgac gtaatcgcga tagcgccagg aaatcagcag
      181 ataggggtcc tgctcgaagc ccgccgtgtc accgtaacgc gccaggtcga gccagtgccg
      241 cccccatttt tcgccataac gcggcgaggc cagcagacgg tcgatctcgt gctcccaggc
      301 gtccggggag cggtcgttca gaaagcggtc gatttcgtct tgcgcgggag gcagcccgag
      361 aagatcgtag gaggcgcggc gtaacagcgt aaggcggtcg gccggcgggg ctttggtaag
      421 cccggcggag gccagtttgc ggtcgatgaa tgcgtcgatg ggattctttt ccggtcccgc
      481 gggaaccgcc ggacgcaccg gcttgcggaa cgcccaccat gtggcggaag cggatgcagc
      541 ggagcctgct gtccactgcg cgccgccgtc gatccaggtc ttgatggcgc cgacttcatc
      601 ggctgtgaga gcgccgttcg gaggcatcga cacatcgcca atatgcgcga cagccttata
      661 caacagactg tcggcggact tgcccggaac caacgcggga ccgcgcgatc cgcccttcaa
      721 caggttgtcg cgcgaggtga ccttgagtcc cgacatcccg ctcgaggttc cgtggcagga
      781 gccgcacttc tgctgaagca aagccggggt gtcggtcgcg gcaaatacgc atgccgaagc
      841 gacgagcggg agcaggaatc cgatcttcat tcaggccgtc tcggtgactt tattctatgt
      901 cacagggtcg gctctgaatc ggagcatgcc gctaccgggc tgcagcctct cgatgtgagc
      961 cgtcagtgcg cgaacgcgga tctcgacagc cgcatctcaa tgcgagaccg agcgccatag
     1021 cgaggtcgat ttcgccagcg gaagcttcac tcccagaatc tccgcgaggg tgggcgcaat
     1081 ctgcagcatg tcgacttcgc ccagtttcgt cggcttgatc cccgttccgt tgaggatcag
     1141 cagcgaccgg taattcacgt gtgtgggcca gaaatcgcac acgccggcgt gcggccctgt
     1201 ccccacggcg gagccgcgct cctcgtcgcg cgccacgtaa tcctgcggtg tatcgaacgc
     1261 cgcgatccac ttgccgagtg acggcgcgcg cgcccgcact tccgccatcg gaacctcgcg
     1321 cgcgattccc gatttgcggg tctctgccgc cagcttgcgg agttccttcg ccacgccggc
     1381 atcgcgcgtt ccgatcagtc cgtcgcgcac ttcaaccgtg cctttcacgc cggcctggcg
     1441 caactgcacg cgcggccgca ccacatgaaa gctgctctgg aagccgtgat ccgacacaat
     1501 ggcgacgatg gttccggcgg gcgctttgcg aacgatttgt ccgaccagat cgtcgtcatt
     1561 ctcgaggatg tcgcgcgcgt agatgctgac cgcggtggtc tcgcgctgct cggcgtcgag
     1621 atcgcccatg tggacgaaca atacgtcagg ctgctcggca ctgagcaggt acatcgcggc
     1681 gtcggcggac gatgtgtcgt cccagagttg cttgtcgaag gcgggaaaca tttgggcgac
     1741 gcggtcggtg agtccggccg ggaagctctt cgcgcccaca tcggcgagcg gcaccgcgtt
     1801 gtcgcggcgc gtttcccaat actcgggaaa attccagtcc acgtgcgcgc cgagtgtgga
     1861 aggccagtac accgtggcga ctttgaggcg gccgttgacg gcggcgctcc agagcgttgg
     1921 ggctttgacc gcatccaccg aaaaaaatgc gtcgccggga cgctcgggca ggccctcggc
     1981 cgtgattccg ttctgccacg gcgagacacc ggtgactaaa gaggcgtgcg acggccaggt
     2041 gtccgacggg accacgccca ccactgtcgc gcttgctcct tctttcatga ggcggcgcag
     2101 attcggggct ttgactttca gggcagggtc gttcaggaag cgcgcgtcga gaccgccgat
     2161 cgagatgacc agcagcttat gcggcgtctg agcggccaac agggcgggaa gaattaccgc
     2221 tgcgagcaaa aggcgcgaca aaacacgatt gtacggcctg gggtggcccg gtttcgcggc
     2281 gggaaatacg ccacattcca gggccttcag tacttaaact ctgtcacgac cacttccacc
     2341 ggcttatcag tcgtgttctg ctcgctgtgc cgggccgcgc cgccctgcgc aatgtctccg
     2401 gccttgtgcg gcgtgacatc caccttgcca tcggaactcg tcacgcgcac caccgcgtcg
     2461 gttaaataaa ccatcacgcg gttgagctga tgctcatgca tgggagtccc ctcgtgaggc
     2521 ccgattttca cgcggaacac acgcacctga tcgttctcga actcgagctt gtaatgcttc
     2581 ggatccactt tcaacggatc gagcgcggtg gtgaccttct ttcccgcccc cggcttcttc
     2641 agctcgatct cgacgatccc caccggttgc tccgtggcca cctccgcgat gtgcttgcct
     2701 tcgatcggac tccatttcac ctcgccctct ttgaaattca ggaccgaagt atggccgtct
     2761 tcgcgtttga aattctgcgc gcccgcgttc ttgtagatca tcacgcggtt ggctttgtgc
     2821 tcgtgcatgg acgtgggctc atgcggctgg accacgacat cgagcacgcg aatgtcgtcg
     2881 ttgtttatgg gcgtcttcac cgtttcgacg gcaaagagca gcgcggcggc cgcgggcagg
     2941 gcgagcagaa cttgttttcg cat
//
LOCUS       DASZMC010000043         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_570069,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000043 DASZMC010000000
VERSION     DASZMC010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570069"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VG274_00855"
     CDS             <1..199
                     /locus_tag="VG274_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05222.1"
                     /translation="EGGMNFLRRVTAGAAHHDFAPSLFPFENRARTGASLLRTSAGTE
                     ICPWGTRWNALQRLALAIGGI"
     gene            complement(899..2299)
                     /locus_tag="VG274_00860"
     CDS             complement(899..2299)
                     /locus_tag="VG274_00860"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWE05223.1"
                     /translation="MNDPLFVAESPRPVFAAEAETQFNAFVHGQPDLEFIDAVIPDLC
                     GALRGKRFPIGEAAHLFSHGMAIPHSIYLMDAHGEMTNAFGRGFSDGDPDGTAWPLPG
                     TLSRVAAEGPPRAQMLMSLRDAAGVPDPGEPRAVLERVLARFADLGLVPVTALELEFY
                     LLDRERLDHRSPQPPLDPRSGARERAVSVFGLDDLDRYEAFLSALAAAARRQGVPLSA
                     ASKEYAAGQFEANLKHQKDARFSADHATFLKQVVKEAARSVGFDATFMAKPYTERAGN
                     GLHIHLSILDRDGGNIFAGADAAGSEQLRHAIGGMQELMPESMAIFAPNQNSYRRFQP
                     DMFAPVNRSWGVNNRSAGMRVPAGPDASRRIEHRVSGADANPYLALAAVLAAAHHGLL
                     KKLDPGEPAKGNVSREPDRALPFTIDDALAKLAKAETLPAYFGARTLELYGETKRIET
                     ERLRRAIPPVEYDYYL"
     gene            complement(2312..>3260)
                     /locus_tag="VG274_00865"
     CDS             complement(2312..>3260)
                     /locus_tag="VG274_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWE05224.1"
                     /translation="LLEGARAGDCVSRLFTRDAAQLLPGESIEAAWLNDEGALRGTGI
                     VARLTTCSYLLVSAQGDPDWIGRAAQLFGVSCRDVAAAQCGLALAGPGAASVLAAAGI
                     GAHVAPLAIVPRNWRGRKLLLSRWWHGYEIWCAPHDALTLWDGLVEAGAAYALTPIGL
                     SAMDVLDLECGIPMSGRDCAPTRDANVNEPLPRMLGLAPLIDEKHVQYNGRSAYVGGP
                     FEQCSLMGLEIESRWPAPHMPIEAGGRNIGRTLHSAYSPALRRAIALALIDEGEALPA
                     RSVSLTLPPVRGRGAFSRVDAILVRLPFLPIAAPIFVEA"
BASE COUNT          590 a         1026 c         1150 g          494 t
ORIGIN      
        1 agagggcggc atgaatttcc tccgacgagt aacggccggc gcggcgcacc atgacttcgc
       61 cccttcgctc ttcccattcg agaaccgagc ccggaccggc gccagcctgt tgcgcacttc
      121 ggccggcacg gaaatctgcc cctgggggac gcgttggaac gctctccagc gtctggcgtt
      181 ggcgattggc gggatttagg gggccagctg gggaagagcg ccagactgat ggatcactca
      241 gtggagtccc ttccggttga gcagcggatc gcgcgctacc gggaaatggt cgctgaagcg
      301 ttgcgggtcg cgaacgacgc gcaagatcag gaagtccgca tgcatcttcg ggaagtggcg
      361 gcggtttggg cggcgttggc ggacaaggca gaacgggagc aagaggagcg cactcacgcc
      421 gctggttcaa gctccgggtc tgatgacgga acgcaagcct ggatgaagga ttgagggcca
      481 accaccatcg cgcgttccgg gcggccacaa aaaggaaccc cgccatccaa ccgaatgacg
      541 gggttccaaa acattcgacg gagacatcca gccctcgcat gctgtcgcga aagcgtcgca
      601 atatgccgca gagagtcctg aggccgcgtt aacagccgcc agagcccgtt aatcgcggtg
      661 agccgtccat tttgtggtgc gcgtcttgcc agtccgggcc gcctcacctt ggcctcgagt
      721 ttcccgacga tcacagccac ccgttccgca acggcccgat gggcggatcg tggcaagcgc
      781 tcagcggtca ccgagccgcc gcgatcgcgc cggcaaagag gaattggctg tgttccgggc
      841 cgcctcgccg accatgcggg aggagtgcgc ggaaggtgga gcgcatgacc gcatgcgctc
      901 acagatagta atcatactcg acgggaggaa tggcgcggcg gaggcgttcg gtctcgatcc
      961 gcttggtttc gccgtagagt tcgagcgtgc gggcgccgaa ataggcggga agcgtttccg
     1021 ctttggcgag cttggccagc gcatcgtcga tggtgaaagg aagcgcgcga tcgggttcgc
     1081 gcgagacgtt gcccttcgcg ggctcccccg gatcgagctt cttgagcagg ccgtgatggg
     1141 cggcggccaa caccgcggcc agcgcgagat aaggattggc gtcggcgccc gaaacgcggt
     1201 gttcgatgcg gcgcgatgcg tcgggccccg caggcacgcg cattccggcg gaccggttgt
     1261 tgacgcccca gctgcggttg acgggggcga acatgtcggg ctggaaacgg cggtaggaat
     1321 tctggttggg cgcgaagatc gccatcgatt ccggcatcaa ctcctgcatc ccgccgatcg
     1381 cgtggcgcaa ttgttccgag cccgccgcgt cggcgccggc gaagatgttg ccgccatcgc
     1441 gatcgagaat cgagagatga atgtgcagcc cgttgccggc acgctccgtg tagggcttgg
     1501 ccatgaaggt ggcgtcgaag cccaccgaac gcgcggcctc cttgaccacc tgcttgagga
     1561 acgtcgcatg atcggcggag aagcgcgcgt ccttctggtg cttcagattg gcctcgaact
     1621 gacccgcggc atattccttg ctggcggcgc tcagcggcac gccctgcctt cgcgcggcgg
     1681 cggcgagcgc cgaaaggaac gcctcgtagc ggtcgagatc gtcgagaccg aagacggaaa
     1741 ccgcgcgttc gcgcgcgccg cttcggggat ccagcggcgg ctgggggctg cgatgatcga
     1801 ggcgctcgcg atcgagcaga tagaattcga gctcgagagc ggtgacggga acgagaccga
     1861 gatccgcgaa gcgcgccagc acgcgctcga gcacagcgcg cggctcgccg ggatcgggaa
     1921 cgcccgcggc gtcgcgcagc gacatcagca tctgcgcacg gggcggccct tccgccgcaa
     1981 cgcgcgacag cgtgccgggg agcggccagg cggtgccgtc gggatcgccg tcgctgaagc
     2041 cccgcccgaa agcgttggtc atctcgccat gcgcgtccat cagatagatg gagtggggga
     2101 tggccatgcc gtgcgagaac agatgcgcgg cttccccgat ggggaaacgc ttgccgcgca
     2161 gcgcgccgca gagatcggga atcaccgcat cgatgaattc gaggtcgggc tggccatgca
     2221 cgaacgcgtt gaattgtgtc tccgcctccg cggcaaaaac agggcgcgga ctctccgcaa
     2281 cgaaaagggg atcgttcatg ccgcgcccga ttcacgcttc cacgaagatg ggagcagcaa
     2341 tgggcaggaa gggcaagcgc acgaggatgg cgtcgacgcg agaaaacgcg ccgcggccgc
     2401 gcaccggcgg cagtgtgagc gagacggagc gggcgggcag cgcctcgccc tcgtcgatca
     2461 gcgccagcgc aatcgcgcgc ctgagcgcgg gcgagtacgc ggaatggagc gtgcggccga
     2521 tgtttcgtcc gcccgcctcg atgggcatat gcggcgcggg ccatcgcgat tcgatttcga
     2581 gtcccatgag cgaacattgc tcgaatggtc cgccgacata cgcggaacgt ccgttgtact
     2641 gcacgtgctt ctcatcgatt agcggtgcaa ggcccagcat gcgtggcagg ggctcgttca
     2701 cattcgcgtc gcgcgtcggc gcgcagtcgc ggcccgacat cgggattccg cattcgagat
     2761 cgagcacgtc catcgcagaa agtccgatgg gcgtcagcgc ataagccgcg ccagcctcca
     2821 cgagcccgtc ccagagcgtg agcgcgtcgt gaggtgcgca ccagatctcg tagccgtgcc
     2881 accagcgcga aagcaggagt ttgcggccac gccagttgcg cggcacgatc gcaagcggcg
     2941 cgacatgcgc gccgatgccg gccgcagcga ggaccgaagc cgcgccgggg ccggccagtg
     3001 cgagcccgca ttgcgcggcg gcaacgtcgc ggcacgaaac gccgaaaagc tgcgcggcgc
     3061 gcccaatcca gtcgggatcg ccttgcgcgg agacgaggag atagctgcat gtggttagcc
     3121 gcgcgacgat tcccgtgcca cgcaacgcgc cctcatcgtt gagccaggcg gcctcgatgc
     3181 tttcgccggg cagaagctgc gccgcgtcgc gggtgaagag ccgcgaaacg caatcaccgg
     3241 cgcgggcgcc ttcgagcagc
//
LOCUS       DASZMC010000044         5012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3865449,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000044 DASZMC010000000
VERSION     DASZMC010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3865449"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1590
                     /locus_tag="VG274_00870"
     CDS             199..1590
                     /locus_tag="VG274_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWE05225.1"
                     /translation="MADATRNRPVRSTLDLIGNTPMVELTRFDAGPCRLFAKLENQNP
                     GGSIKDRIGLSMIEAAERGGCLAPGGTIVEATAGNTGLGLALAGILKGYHVLLIVPDK
                     MTREKIQHLRALGAEVRITRSDVGKGHPDYYQDKAEKLARETGAFFVNQFANPANPKA
                     HEETTGPEIWEQMDRDLQAIVIGVGSGGTLTGIGRFLRRVAPNVEIVLADPAGSILAP
                     YVRTGELVQAGSWAAEGIGEDFIPPNADLSLVSRVYSISDKEGIEAARALLAKEGILA
                     GSSSGTLLAAALRYCREQTTPKRVVTLVPDSGNKYLSKIYNDHWIIEQGLADRELHGD
                     LRDLITRRFDRGGAVTVAPGDTLLVAYNRMRDADVSQLPVVDEECVVGILDESDILAF
                     VEGKDAHRVQRFRERVDRAMTRELHTMQITDRLDALPPLFERNEVAIVLDGARFVGLI
                     TRVDLLNHLRLSP"
     gene            1587..2753
                     /locus_tag="VG274_00875"
     CDS             1587..2753
                     /locus_tag="VG274_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HWE05226.1"
                     /translation="MSARRNRLAFATRTIHGGQNHDPLTGAVMVPIYATSTYAQESPG
                     VHKGYEYSRSDNPTRAAFERCIADLETGTAAFAFASGLAAMATVLELLDSGDHVVASD
                     DLYGGSFRLFDRVKRRSAGLGFSFVDLSDPAALESALTDETKMIWVETPTNPLLRLAD
                     LEAIALLARQRGILCVADNTFATPYAQRPLELGFDIVVHSTTKYLNGHSDMVGGVAVA
                     GENRELAERLKFLQNAVGAIQGPFDSFLALRGVKTLALRMERHSSNAMRIARWLETRG
                     EVARVVYPGLDSHPQHALALRQMSAFGGIVTVILRRDLDGTKRFLERCRLFTLAESLG
                     GVESLIEHPGIMTHASLPPGQRARLGISDSLVRLSCGIEDADDLIADLEQALAA"
     gene            complement(2904..4568)
                     /gene="hutU"
                     /locus_tag="VG274_00880"
     CDS             complement(2904..4568)
                     /gene="hutU"
                     /locus_tag="VG274_00880"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HWE05227.1"
                     /translation="MTRLDNSRSIRAPRGTQLNAKSWLTEAPLRMLMNNLDADVAERP
                     EELVVYGGIGRAARDWESFDRIVATLKRLEGEETLLVQSGKPVGVFQTHADAPRVLIA
                     NSNLVPHWANWDVFHALDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFAELGRRHYH
                     GSLAGRWILTAGLGGMGGAQPLAAVMAGASMLAIECQPSRIEMRLRTKYLDVQAQSLD
                     EGLEMIARAVREKKSLSVGLLGNAADVFPELVRRGVKPDAVTDQTSAHDPLNGYLPKG
                     WTLAQWESVRASDPRSVERAAKHSMAEQVRAMLAFHRMGVPTVDYGNNIRQMAKEDGV
                     ADAFDFPGFVPAYIRPLFCRGIGPFRWCALSGDPEDIYRTDAKVKELLPNDAHLHHWL
                     DMARQRIRFQGLPARICWLGLGDRHRVGLAFNEMVAKGELKAPIVIGRDHLDSGSVAS
                     PNRETEAMKDGSDAVSDWPLLNALLNCASGATWVSIHHGGGVGMGFSQHAGMVIVCDG
                     TEDAARRIARVLWNDPASGVMRHADAGYESAIACAKEHGLDLPGVA"
     gene            complement(4565..4849)
                     /locus_tag="VG274_00885"
     CDS             complement(4565..4849)
                     /locus_tag="VG274_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HWE05228.1"
                     /translation="MEVESIVSRINRGPNFTRVTNGRFKGGYTTRHYGEPARGVHAVQ
                     FELAMRSYMQEPQSPSPENWPPPYDPARAAPLRTILQRILESCLTFASPT"
BASE COUNT          863 a         1686 c         1582 g          881 t
ORIGIN      
        1 ctccgcttcg cttcgcaaac cacctccccc acgatggggg aggaaaatta aggcgcgctt
       61 cgcgccgaag cacgaggcga tttgtgcccg cattggcgct ggagaggaaa tccggtcctc
      121 acaggaatta acgcgtttcg aactgctctc gggccttttg tttcgctatg gtgcagcggc
      181 aaagacagga actcccccat ggcggatgca acccgcaaca gaccggtgcg ctcgacgctc
      241 gacttgatcg gaaacacgcc catggtggag ctgacgcggt tcgatgccgg gccgtgccgc
      301 ctgttcgcca agcttgagaa ccagaatccc ggcgggtcga tcaaggaccg catcggcctt
      361 tcgatgatcg aggccgccga acgcggcggc tgtctcgctc ccggcggcac gatcgtcgag
      421 gcgaccgccg gcaatacggg cctcggcctt gcactggctg gaatcctcaa aggctatcac
      481 gtgcttctca tcgtccccga caagatgacg cgggagaaga ttcagcatct gcgcgcgctg
      541 ggcgccgaag tgcgcatcac gcgttccgac gtcggcaagg gccatcccga ctattaccag
      601 gacaaggccg aaaagctcgc gcgcgagacg ggcgccttct tcgtcaatca attcgccaat
      661 cctgcaaatc cgaaggccca cgaagagacg acgggtcccg aaatctggga gcagatggat
      721 cgcgatctgc aggcgatcgt catcggcgtg ggatcgggcg gcacgctgac gggcatcggc
      781 cgcttcctgc gccgcgtggc gcccaacgtc gagatcgtgc tcgccgatcc tgccggctcg
      841 atcctcgcgc cctatgtgcg caccggcgaa ctcgtgcagg ccggcagctg ggccgccgaa
      901 ggcatcggcg aagatttcat tccgccgaac gccgacctct cgctcgtgtc gcgcgtctat
      961 tcgatctccg acaaggaggg aatcgaggcg gcgcgcgcgc tgctcgcgaa ggaaggcatt
     1021 ctcgcgggct cgtcttcggg cacgctgctc gctgccgccc tgcgctattg ccgcgagcag
     1081 acaacgccga agcgcgtcgt cacactcgtg cccgacagcg gcaacaaata tctttccaag
     1141 atctacaacg accattggat catcgagcag ggactcgcgg accgcgaact gcatggcgat
     1201 ctgcgcgatc tcatcacccg gcgcttcgac cgcggtggcg ccgtcaccgt ggcgcccggc
     1261 gatacgctgc tcgtcgccta caaccgaatg cgcgacgccg atgtgtcgca gcttcccgtg
     1321 gtcgacgaag aatgcgtggt gggaatcctc gacgagagcg acatcctggc ctttgtcgaa
     1381 ggaaaggacg cgcaccgcgt gcagcgtttt cgtgagcgcg tggaccgggc gatgacgcgc
     1441 gagctccaca ccatgcagat caccgacagg ctcgatgcgc tgcccccgct gttcgagcgc
     1501 aacgaagtgg cgatcgtgct cgacggcgcg cgtttcgtcg gcctcatcac ccgcgtcgat
     1561 ctcctcaacc atcttaggct cagcccgtga gcgcgcgccg caaccgcctg gccttcgcga
     1621 cccgcaccat tcatggcgga caaaatcacg atccactcac cggcgcggtg atggtgccga
     1681 tctacgcgac ctcgacctac gcgcaggaaa gccccggcgt gcacaagggc tacgaatatt
     1741 cccgcagcga caatcccaca cgcgccgctt tcgagcgctg catcgccgat ctcgaaaccg
     1801 gcaccgccgc ctttgccttt gcctcgggcc ttgcggcgat ggcgaccgtg ctcgaactgc
     1861 tcgacagcgg cgaccacgtg gtggcgagcg acgatctcta tggcggctcg ttccgcctct
     1921 tcgaccgcgt gaagcggcgc tccgccgggc tgggcttcag cttcgtcgat ctgagcgatc
     1981 cggccgcact cgaaagcgcg ctcaccgacg agacgaaaat gatctgggtg gagacgccga
     2041 ccaatccgct gctgaggctg gcggatctcg aagcgatcgc gcttctggcg cggcagcgcg
     2101 gcattctctg cgtcgccgac aacactttcg ccacgcccta tgcgcagcgt ccgctcgaac
     2161 tgggtttcga catcgtggtc cattccacga ccaaatatct gaacgggcac tccgacatgg
     2221 tcggcggcgt ggccgtggca ggcgaaaacc gggaactggc ggagcgcctc aaattcctgc
     2281 agaacgcggt cggcgcgatc cagggcccgt tcgattcgtt cctcgcgctg cgcggcgtca
     2341 agactctcgc gctgcgcatg gagcgccatt cgtcgaacgc gatgaggatc gcccgctggc
     2401 tggaaacgcg cggggaagtc gcgcgcgtcg tctatcccgg tctcgacagc catccgcagc
     2461 acgcgcttgc tttgcggcag atgagcgcct tcggcggcat cgtgaccgtc atcctgcggc
     2521 gcgatctcga cggcacgaag cgctttctcg aacgctgccg cctgttcacc ctcgccgaga
     2581 gtctcggcgg cgtcgagagc ctgatcgagc atcccggcat catgacccat gcctcgctgc
     2641 cgcctggcca gcgcgcccgt ctcggcatct ccgattcgct ggtgcgcctc tcctgcggca
     2701 tcgaagacgc cgacgacctc attgccgatc tggaacaggc gctcgccgcg tgacgcacga
     2761 tttcctcccc cgcacttcgc gggggaggtg tcgggcgagc gcagcgagca cgacggaggg
     2821 ggtgagtggg gcgccgcaag tggccccctc cgccccttcg gggcacctcc cccacgcctg
     2881 cggcgcgggg gaggaaaaag cggctacgca actcccggca aatccagccc gtgttccttt
     2941 gcgcaggcaa tcgcgctttc gtagccggcg tcggcatggc gcatgacgcc gctggcggga
     3001 tcgttccaca gcacgcgggc gatgcggcgc gccgcgtcct cagttccatc gcagacgatc
     3061 accattcccg cgtgctgcga aaatcccatg ccgacgccgc cgccgtgatg gatcgacacc
     3121 caggtggcgc cgctggcgca attgaggagg gcgttcagga gcggccagtc cgacaccgcg
     3181 tcggagccgt ccttcatcgc ttcggtctcg cggttgggtg acgcgacgga accggaatcg
     3241 agatggtcgc ggccgatgac gatcggcgct ttcagctcgc ccttcgccac catttcgttg
     3301 aaggcgaggc cgacgcggtg acggtcgccg agtccgagcc agcagatgcg cgcaggcaat
     3361 ccctggaaac ggatgcgctg gcgcgccatg tcgagccagt ggtgcagatg cgcgtcgttg
     3421 ggcagcagtt ccttcacctt cgcgtcggtg cgatagatgt cttcgggatc tcccgacaac
     3481 gcgcaccagc gaaacggacc gatgccgcgg cagaacagcg ggcggatata ggcaggcacg
     3541 aaaccgggaa aatcgaacgc gtccgcgacg ccgtcttcct tcgccatctg gcggatgttg
     3601 ttgccgtaat cgacggtcgg cacgcccatg cgatggaagg cgagcatcgc gcgcacttgc
     3661 tccgccatcg aatgtttcgc cgcccgctcc acacttctgg gatcactggc gcggacgctc
     3721 tcccattgcg caagcgtcca gcccttcggc aaataaccgt tcaaaggatc atgcgccgat
     3781 gtctggtcgg tgaccgcgtc gggtttcacg ccgcgccgca cgagttccgg gaacacatcc
     3841 gccgcgttgc cgagcaggcc gacggagagc gattttttct cgcgcaccgc gcgcgcgatc
     3901 atttcgagcc cctcgtcgag cgactgcgcc tgaacgtcga gatatttcgt gcgcaggcgc
     3961 atctcgatgc gcgaaggctg gcattcgatg gcgagcatcg atgcgcccgc catcaccgcg
     4021 gcgagcggct gcgcgccacc catgccgcca aggcccgccg tgaggatcca gcgtccggcg
     4081 agtgatccat ggtaatggcg ccgccccagc tccgcaaacg tctcgtaggt gccctgaacg
     4141 attccctggc tgccgatata gatccagctg cccgccgtca tctggccgta catcatcagg
     4201 cccttgcggt cgagcgcgtg aaatacgtcc cagttcgccc aatgcggcac cagattggaa
     4261 ttggcgatca acacgcgcgg cgcgtcggca tgggtttgga acacgcccac gggtttgccc
     4321 gattgcacga gcagcgtctc ctcgccttcg agacgtttta gtgtcgccac gatgcggtcg
     4381 aaactttccc agtcgcgcgc cgcccgcccg atgccgccat agacgaccag ctcttcgggc
     4441 cgctccgcca cgtccgcgtc gagattgttc atcagcatgc gcaacggcgc ttcggtcagc
     4501 cagctcttcg cgttgagctg cgtgccgcgc ggtgcgcgga tggagcggga attgtcgaga
     4561 cgggtcatgt tggactcgcg aaggtgaggc aggattcgag aatgcgctgc aggattgtgc
     4621 gcagcggtgc cgcgcgcgcg ggatcgtaag gcggcggcca gttttccggc gacggagatt
     4681 gcggctcctg catgtaggag cgcatggcga gttcgaactg caccgcatgc acgccgcgcg
     4741 ccggctcgcc ataatgccgc gtcgtatatc cgcccttgaa gcgcccgttg gttacgcggg
     4801 tgaaattcgg gccgcggttt attctggaga caatggattc aacttccatc ttacctcccc
     4861 cttgtgggga ggtcgaagcg cgcccttcgc gcgcttcggg tgggggtctc cgtctctcca
     4921 tggataggcc gcgcgcccca tccgaaatcg ctgcgcgatt tcgacctccc cacgaggggg
     4981 aggtaaattg ttcgtcgcat gcacgctcaa tc
//
LOCUS       DASZMC010000045         4596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2222516,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000045 DASZMC010000000
VERSION     DASZMC010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222516"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VG274_00890"
     CDS             complement(<1..966)
                     /locus_tag="VG274_00890"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWE05229.1"
                     /translation="MAFEHVDVLIVGAGLSGIGAAHHLQRRCLRKSYAILEGRGAIGG
                     TWDLFRYPGIRSDSDMYTMGYSFRPWTNPKAIADGPSILGYIRDTAEEAGIGRHIRFN
                     NWVTRASWSSENAMWTVEAVRKSARNGAPDEAVRFTCTFLFMCSGYYRYSAGYLPEFS
                     GTGRFKGQIVHPQKWPEDLNFANKRVVVIGSGATAVTLVPEMSKTAAHVTMLQRSPTY
                     IVSRPEQDAVANWLRRHLPAQAAYAITRWRLVLLGMWFYRLCKRQPERIKELIMKGVR
                     AELGPDYDVATHFTPRYNPWDQRLCLVPDADLFRAMKSGRASIVTD"
     gene            complement(1174..1875)
                     /locus_tag="VG274_00895"
     CDS             complement(1174..1875)
                     /locus_tag="VG274_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HWE05230.1"
                     /translation="MNMSERFKGFPKDFFAFFRELAAHNERGWFESNKPRFRDSVQAP
                     MSDFIAAMAPRLAKISKHFAADPRPNGGSMFRIYRDVRFSKDKRPYKEHAACHFRHAL
                     GKDAHAPGFYMHFSPNEVFFGGGLWMPPPDALAKIREGIAAKRVGWKQLLANKSFAKY
                     FGGVRGEALVRPPRGFDPEHPFIEDIKRKSFYAMREESPKLATSPELVDEVAVTFAAL
                     SPLMKFLCSAIDVPY"
     gene            complement(2097..3656)
                     /locus_tag="VG274_00900"
     CDS             complement(2097..3656)
                     /locus_tag="VG274_00900"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWE05231.1"
                     /translation="MHPSRLQIRPIATILATLLILGAAIYSTLMTIARVGDAHQREME
                     QRVVQSALNQQRMNLGNTLVQQAFWDSAYDAVGPHLNPKWLDKNLGFSAETAGVPFTA
                     IFDRDAKPVYHYSASATAADANSLQASRSLAQLAERALAVPVLPPAPLSAIVRVRNHL
                     YLAAAERIVPNDARAGLPLARSYVLTNLVPLNAAMLGNLQSGFGIAPLSISASPGPDL
                     ADVPLVAVNAAPLGYLEWRPARPGREFADAAAPMALSCFALLAALQLVVLRSWLQAAQ
                     RMRDEGMAKTMFLANASHELRTPLNAIIGFSECMDGEMFGPLDARYREYARDIRTSGQ
                     LLLGIVNDVLDLTHLNSAAEVVMEPVKLGEALGGALRMLREYAKGDRICIDFVDHSEG
                     AEVAANEKALSQILLNLGSNAVKFSPPHTTVDIALRRGADFVELIVRDHGAGISAEKL
                     RFIGQPFFQANETSTRKPGSGLGLAIVKKLTQRLGGEFAIASSPGEGTTVTVRLPQHR
                     PPPEPLHARAA"
     gene            3928..4563
                     /locus_tag="VG274_00905"
     CDS             3928..4563
                     /locus_tag="VG274_00905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE05232.1"
                     /translation="MSLQPPLSAAHAAPSPARPAQGADAAALAATLARAFVDDPFVSH
                     LVPGRDARVRKLPGLFGLLFKLGRPYGACDVTDKTEAAALWRPPGAWHIPLRHYLTNG
                     PLLLGIFGTNTLRALGAMDTLERRHPREPHWYLQAIGTDPAFQGKGYGGVLLRHRLAL
                     IDAAGLPAYLEASRESNVPLYANFGFVLTGEVRIADGPTLYPMWRAPERRK"
BASE COUNT          849 a         1425 c         1494 g          828 t
ORIGIN      
        1 atcggtgacg atggaggcgc ggccgctctt catcgcgcgg aagagatcgg catccggcac
       61 gagacacagc cgctggtccc acgggttgta gcgcggcgtg aaatgcgtcg cgacgtcgta
      121 gtcgggcccg agctccgcgc gcacgccttt catgatcagt tcttttatgc gctcgggctg
      181 gcgcttgcag aggcggtaga accacatgcc gaggagaacg agccgccagc gcgtgatcgc
      241 ataggcggcc tgcgccggca gatggcggcg cagccagttc gcgaccgcat cctgttcggg
      301 gcgcgagaca atgtatgtcg gcgagcgctg caacatcgtc acatgtgcgg cggtcttcga
      361 catttcgggg acgagcgtca ccgccgtcgc gccgctgccg atgacgacga ctcgcttgtt
      421 tgcaaaattc aaatcttccg gccatttctg cggatgaacg atttggccct tgaagcggcc
      481 ggttccggaa aattcgggaa gatagcctgc ggaataccga tagtagcccg aacacatgaa
      541 gaggaaagtg caggtgaagc gcacagcctc gtccggcgcg ccgttccgcg cggacttgcg
      601 caccgcttcg acggtccaca tcgcgttttc cgaagaccac gaagcacgcg tcacccaatt
      661 gttgaatcga atatgccggc cgatacctgc ttcctctgcc gtgtcgcgga tgtagccgag
      721 gatcgagggg ccgtcggcaa ttgccttggg gttcgtccac gggcgaaagg aatagcccat
      781 cgtgtacatg tcggaatcgg agcgaatccc cgggtaacgg aagaggtccc aggtgccacc
      841 gatcgcgccg cggccttcaa ggatcgcgta actcttgcgg agacaccgcc tctgcaaatg
      901 atgcgccgcg ccaatgccgg aaagccccgc gccaacgatc agcacatcga catgttcgaa
      961 cgccaccctc aatcgtccct cgattgacga gcgagctaat gttttcgcgg gccggaggtc
     1021 aaggcggctc gatggcgccc ttcaattata acgtgtcagg cccgcaggag cgggcatcca
     1081 ggagcaggcg acgtgcgttt gtctctggat tcccgctcct gcgggaatga catggcgtta
     1141 ttcgttcagt atgtgcgctt tcaacgcgcc ttctcaatac ggcacatcga ttgcgctgca
     1201 gaggaacttc atcagcgggc ttaacgccgc aaaggtgacg gcgacttcgt cgacaagttc
     1261 gggcgaagtg gcaagcttcg ggctctcctc gcgcatggcg tagaaactct tcctcttgat
     1321 gtcttcgatg aagggatgtt ccggatcaaa accgcgtggc ggacggacca gcgcctcgcc
     1381 gcgcacgccg ccaaaatatt tggcgaaaga tttgttcgcc agcagttgct tccacccgac
     1441 gcgcttcgcc gcgatgcctt cgcggatctt tgccaacgcg tccggcggcg gcatccacaa
     1501 tccgccgccg aagaacacct cattaggcga aaaatgcatg tagaaacccg gcgcatgcgc
     1561 gtccttgccg agtgcatggc ggaaatggca ggcggcatgc tctttgtacg gacgtttgtc
     1621 cttggaaaag cgcacgtcgc gatagatgcg gaacatcgag ccgccgttcg gccgtggatc
     1681 ggcggcgaag tgcttggaga tcttcgcgag cctcggcgcc atcgcggcaa tgaaatcgct
     1741 catcggcgcc tgcacgctgt cgcggaagcg cggcttgttg gattcgaacc agccgcgttc
     1801 gttgtgcgcc gccaattcgc ggaagaaggc gaagaagtct ttggggaaac ctttgaagcg
     1861 ttccgacatg ttcattcctg caatctctcg ggtgaacaga ctatcgtatt gctgcgcaag
     1921 tcgcgcgccg tttccccctc cgcccgcttc gcgggcacct cccccacgcg cgatgcgcgc
     1981 gggggaggaa aatgaagggt gcacaaagca aagcctttcc tccgccgcac gaaagccttt
     2041 cctcctccgc gcgcatagcg cgtgggggag gtgaccgcga agcgggcgga ggggggctat
     2101 gccgcgcggg cgtgcagggg ctcgggcggc gggcgatgct gcggcaggcg caccgtcacc
     2161 gtggtgccct cgcccggcga actcgcaatg gcaaattcgc cgccgagtcg ctgggtcagt
     2221 tttttgacga tggcaaggcc gagaccgctg cccggcttgc gcgtggaggt ctcattcgcc
     2281 tggaagaagg gctggccgat gaatcgcaac ttctccgcgg agatgccggc gccatggtcg
     2341 cgcacgatca gctcgacgaa atcggcaccg cggcgcaggg cgatatcgac tgtggtgtgc
     2401 ggcggactga acttgaccgc gttggagccg aggttcagca gaatctggct gagcgccttt
     2461 tcgttggcgg ccacttcggc gccttccgag tggtcgacaa aatcgatgca gatgcggtcg
     2521 cccttggcgt attcgcgcag catgcgcagg gcgccaccca gcgcctcgcc cagcttgacc
     2581 ggctccatga cgacttcggc ggcactgttg agatgggtga gatccagcac gtcgttgacg
     2641 attccgagca gcagctggcc gctcgtcctg atgtcgcgcg cgtattcgcg atagcgcgcg
     2701 tcgagcggcc cgaacatctc gccgtccatg cattcggaaa aaccgataat ggcgttcagc
     2761 ggcgttcgaa gctcgtggct cgcattggcc aggaacatcg tcttggccat gccctcgtcg
     2821 cgcatacgct gggcggcttg cagccacgac cgcagcacga cgagttgaag cgccgcgagg
     2881 agcgcgaagc aggaaagcgc catgggcgcc gccgcgtcgg caaactctct gcccggtctt
     2941 gccgggcgcc attccaggta accaagcgga gcggcgttga cggcgacgag cgggacgtcg
     3001 gcgagatccg gacccggcga tgccgaaatg ctgagtggcg cgatgccgaa tcccgactgc
     3061 aggttgccca gcatggccgc gttgagcggc acgagattgg tcagaacata gcttcgggcg
     3121 agaggcagcc cggcgcgcgc gtcattggga acgatacgct ccgcggccgc aagatagaga
     3181 tggttgcgaa cgcgcacgat ggcggatagc ggcgccggcg gcagcacggg cacggcgagc
     3241 gcccgctccg cgagttgcgc gagcgaccgg ctcgcctgga gagaattcgc gtcggctgcc
     3301 gtcgcgcttg cgctgtaatg ataaacgggc ttggcgtccc gatcgaagat ggcggtaaag
     3361 ggcacgcctg cagtctcggc ggaaaagccc agattcttgt caagccattt gggattcaga
     3421 tgcggcccga cggcgtcgta ggcgctgtcc caaaaggcct gctgcaccag cgtattgccg
     3481 agattcatgc gctgctgatt gagcgccgac tggacgacgc gctgctccat ctcgcgctga
     3541 tgcgcgtcgc caacgcgcgc aatcgtcatc agcgtcgaat agatcgccgc gccgaggata
     3601 agaagagtgg cgaggatggt cgcgatcggc cggatctgga gtcgactggg gtgcattgct
     3661 cgacgtttcg gggttggaac cgacgcgcca atttctatcg caattgaatt gcagccggat
     3721 taacggccga aacgggaaag atacgatccg ttaatgtgtt ggaaacgcga tcccccgcaa
     3781 accgcatcgc acataccccc gcacaccggt cttgcgcgcc gccgcggcca tcacccgaca
     3841 agcaccattc cgcaatcttt cgcccacgag acatcggttg agtatttcaa tatgtacaat
     3901 cgccgaacaa cgaggcgcga tgagtcgatg agccttcaac cgcccctttc cgcagcgcat
     3961 gcggcgccgt cgccggcgcg cccggcccag ggcgcggacg ccgccgctct tgcggccacg
     4021 ttggcgcgcg cctttgtgga cgatccgttc gtctcccatc tcgtgcccgg acgtgacgcg
     4081 cgcgttcgca agcttcccgg cctgttcggt ctgctgttca aactgggccg gccctacggc
     4141 gcctgcgacg tgacggacaa aaccgaagcg gccgcgctct ggcggccgcc cggcgcatgg
     4201 cacatcccgc tccggcatta tctgacgaac gggccgttgc tgctcggcat tttcggcacc
     4261 aacacgctgc gcgccctggg cgcgatggat acgctcgagc ggcggcaccc gcgcgagccg
     4321 cactggtatc tgcaggcgat cggcaccgat ccggcattcc aggggaaggg ctatgggggc
     4381 gttctgctgc gtcatcgcct ggcgctgatc gatgcggcgg gcctgcccgc ctatctggaa
     4441 gcgagcaggg aatcgaacgt tccactctac gccaatttcg gcttcgtgct taccggagag
     4501 gtcaggattg ccgacggccc cacgctctat ccgatgtggc gcgcgcccga acgcaggaag
     4561 tgatgagtgg tgcgcgagaa tgcgtggggc gaaggc
//
LOCUS       DASZMC010000046         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3323794,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000046 DASZMC010000000
VERSION     DASZMC010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3323794"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2454)
                     /locus_tag="VG274_00910"
     CDS             complement(<1..2454)
                     /locus_tag="VG274_00910"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWE05233.1"
                     /translation="MTESPRRLAPRIAGALAACLLLAFAGPAQPAAPADARNVLRATL
                     GNGLRVIIVRNRLAPVAATAVNYLVGSDEAPVGFPGTAHAQEHMMFRGSPGLTADQLA
                     DIGSEMGGNFNANTRESLTQYLYTVPAGYLDVALHIEAARMQDVLDRQADWDVERGAI
                     EQEVAQDLSNPIYVLYVKLRAALFAGTPYEHDALGTRPSFDKTSGAMLKIFHDAWYAP
                     NNAILIVVGDVDPAATLSEIKSLFGTIRPKKLPPKPKFVLRAAPSLNLSADTDQPEGT
                     LLVALRTPGLDSRDFPALEVLSDVLSNHRFDLYGLVPKGEASDASFALDALPKAGVGY
                     AYVRFPAGSDPRAIAGEVKEILARVAKEGVAPGLVDAAKLQERREAEFQKNSIADLAG
                     VWSDAVALYGLRSPDDDLVRIEKVTVADVDHVAREILVMDRVSTAILSPKGSGAPVAS
                     AGGYGGQENIALGEAVPTTLPSWAEAALSRLEAPRSTLSPVVTTLSNGMTLIVQPENV
                     SDTVTVYGHIRNRPETETPKGQEGVADVLNQLFPFGTEQLDRVAYQEALDRIGAQEQP
                     GTDFSVQVLTKDFDRGTALLADNELHPRLPEQAFSVVRERVAQIAAATLRSPAHLTYH
                     ALRAGLFPKDDPSLREATPESVHALTLDDVRAYYHTVFRPDLTTLIVVGHVDPAEARA
                     VAEKYFGGWMASGPKPDTDLPLTPANVAATVAVPDASRVQDNVYLAQTLSMTRTDPDY
                     YALELGNSVLAGGFYASRLSIDLRKTSGLVYSVNSAMQSGRTRSIWLVNYASDPDKVK
                     RAAAIAAEDLHRMQTTPVGD"
     gene            complement(2567..2947)
                     /locus_tag="VG274_00915"
     CDS             complement(2567..2947)
                     /locus_tag="VG274_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWE05234.1"
                     /translation="MRAIDTNVLVRLIARDDPEQTESAEEFVSKGAWISQLVLAETIW
                     ILDSAYERGRGEIATAIEMLLNRAQLSVQDADVVAAALAVFRSRSAVSFSNALIVESA
                     RRSGHLPVATFDRDVAKLDGVQGF"
BASE COUNT          539 a          974 c         1000 g          535 t
ORIGIN      
        1 gtcgccgacg ggcgtggtct gcatccggtg caaatcctcg gccgcaattg cggcggcgcg
       61 cttcaccttg tcgggatcgc tggcataatt gaccagccag atgctccgtg tacggccgga
      121 ctgcatcgcc gaattcaccg aatagacgag gccgctcgtt ttccgcagat cgatgctgag
      181 ccgcgaggcg tagaagccgc ccgccagcac ggagttgccg agctcgagtg cgtagtagtc
      241 gggatcggtg cgcgtcatcg aaagcgtctg ggcgagatag acattgtcct gcacgcggct
      301 cgcgtcgggc acggcgacgg tcgcagcaac attggccggt gtgagcggca gatccgtgtc
      361 cggcttcggc ccgctcgcca tccacccgcc gaaatatttc tcggcaacgg ctctggcttc
      421 cgccggatcg acatggccga cgacgatcag cgtcgtcagg tcgggccgga acacggtgtg
      481 gtaataggcg cgcacgtcgt cgagcgtgag cgcgtgcacg gattccggcg tcgcttcgcg
      541 caacgacgga tcgtctttcg gaaacaggcc ggcccgcaac gcgtggtagg tcagatgcgc
      601 gggactgcgc agcgtcgccg cggcaatctg cgccacgcgc tcgcgcacca cgctaaaggc
      661 ctgctcgggc aggcgcggat gcagctcatt gtctgcgagc agcgccgttc cgcggtcgaa
      721 atccttcgtc agcacctgga cggaaaaatc cgttcccggc tgctcctgcg cgccgatgcg
      781 gtcgagagcc tcctgatagg cgacgcggtc gagctgctcg gtgccaaagg gaaaaagctg
      841 gttcagcacg tcggcgacgc cttcctggcc tttcggcgtc tcggtctcgg ggcggttgcg
      901 gatatggccg tagaccgtga ccgtgtcgct gacattctcg ggctggacga tcagcgtcat
      961 gccattcgaa agcgtggtga cgaccggaga gagcgtggaa cgcggcgctt cgagacggct
     1021 gagcgccgct tccgcccatg aaggcagggt cgtgggcacg gcctcgccca gtgcgatgtt
     1081 ctcctgccca ccataaccgc cggccgaggc gacaggcgcg ccggagcctt tgggactgag
     1141 gatcgcggtc gagacgcggt ccatgacaag gatctcgcgt gccacgtgat cgacatcggc
     1201 caccgttacc ttctcgatgc ggacgaggtc gtcatcgggc gatcgcaaac cgtaaagcgc
     1261 caccgcgtcg gaccacacgc cggcgagatc ggcgatcgaa ttcttctgga attccgcttc
     1321 gcgccgctcc tgcagcttcg ccgcatcgac gagcccgggc gcaactccct ccttcgccac
     1381 gcgcgcgaga atttctttca cctcgccggc gatcgccctg ggatcgctgc cggcgggaaa
     1441 ccgcacatag gcgtaaccca cgcccgcctt cggcaacgca tcgagcgcga agctcgcatc
     1501 gctcgcctcg cctttgggaa cgaggccgta gagatcgaag cgatggttgc tcagcacgtc
     1561 ggagagcact tcgagggcgg ggaaatccct gctgtcgaga ccgggggtgc gcagcgccac
     1621 cagcagcgtg ccttcgggct ggtcggtatc ggcgctgaga ttgagcgaag gcgcggcacg
     1681 cagcacaaat ttaggcttcg gcggaagctt cttcggcctg atcgtaccga agagagattt
     1741 gatttccgac agcgtcgccg ccggatcgac gtcgccgacc acgatcagga tggcgttgtt
     1801 cggcgcatac caggcgtcgt gaaaaatctt cagcatcgcg ccgctcgtct tgtcgaagga
     1861 tggccgcgtg ccgagcgcat cgtgctcgta gggcgttccc gcgaacaacg cggcgcgcaa
     1921 tttcacatag aggacgtaga tcggattgga gaggtcctgc gccacctcct gttcgatggc
     1981 gccccgctcg acgtcccagt ccgcctgacg gtcgagcacg tcctgcatgc gcgccgcctc
     2041 gatgtgcagc gccacgtcga gatagccggc aggaaccgta tagagatatt gcgtcaggct
     2101 ctcgcgggtg ttggcattga agtttccgcc catctcgctg ccgatatcgg cgagctggtc
     2161 ggcggtaagg ccggggctgc cgcggaacat catgtgctcc tgcgcgtgcg cggttccggg
     2221 aaagccgacc ggcgcttcgt ccgagcccac gagataattg accgcggtcg cggcgactgg
     2281 cgcgagccgg ttgcgcacga tgatcacgcg cagaccgttc cccagcgtcg cgcggagcac
     2341 attgcgggcg tccgccgggg cggccggttg tgcaggtccg gcaaaagcca acaaaaggca
     2401 cgccgcaagc gcgcccgcga tacgaggcgc caggcgccgc ggtgattcag tcatgacttc
     2461 tccgaaacga tggggcacga aggccgcgtc aatcgcaggt tccggtgcgc gttgcaaaca
     2521 ccgggctgga gcgcccttct cttggcgcac ccggccggca tacccgctaa aaaccttgga
     2581 ccccgtccag cttcgccacg tcgcgatcga acgtggccac gggcagatga ccgctccggc
     2641 gggcggactc gacaatgaga gcgttggaga agctcaccgc agaccgtgag cgaaaaacgg
     2701 ccagagctgc agcgacgaca tcggcgtcct ggacggagag ttgcgcacgg ttgagcagca
     2761 tttcgatggc ggtcgcgatc tcgcctcgtc cgcgctcata cgccgaatcg aggatccaaa
     2821 tcgtttcggc caacacgagc tgcgaaatcc atgcgccctt cgaaacgaat tcctccgccg
     2881 attcagtctg ttccggatcg tcacgggcga tcaggcgcac gagcacattc gtgtcaattg
     2941 cgcgcatggc gctttcgcat gcgcgcgcga atgcattcct tcagttcctt gactgtcttc
     3001 ggtttgggag cgccgtgtgg aaagagggcg gcatgaattt cctccgac
//
LOCUS       DASZMC010000047         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3324251,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000047 DASZMC010000000
VERSION     DASZMC010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3324251"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VG274_00920"
     CDS             complement(<1..889)
                     /locus_tag="VG274_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05235.1"
                     /translation="MASSTEGRWALLTTSLTGLIAGSIAWMAVGTMPPMHARGFGTGH
                     PTTFLPYQLFQKLAQSTNQASINPFGISVSRKPAAQPAKLDDNLAAQDAADDENKAPG
                     VETRTITMDRGDTLAGALEDAGVSSDEANAAVTALGRVYNSRSLRAGQSFDVTYSTDP
                     QAVAAANEASNNAFDGSDEDDANANGAISPATTTPVNHLISINFSPSVEHDVSITRDA
                     NNAFTANDVEKKLEAHIHRAGATIEGSLYLSAMRAGIPADVVVEMIHMFSYEVDFQRD
                     IKPGDTFEVFYNYYYTPEGQ"
     gene            complement(1228..2094)
                     /locus_tag="VG274_00925"
     CDS             complement(1228..2094)
                     /locus_tag="VG274_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HWE05236.1"
                     /translation="MDELRRALKGAPAGLISDALADTEDHLNNEISHNPEIPESQILA
                     TVIDTYGTPQEIAEEYRDMEATIGGPFPKSDDAPERRYGFFNVVSDPRTYGALLYMLL
                     SLATGIFYFTWTVVGISLTLGFAILIIGIPFALLFIGSIRVLSHVEGRIVEGLLGVRM
                     PRRLPPTTQSDETIWTRIKDGLSDSRTWSSMFYFLLKLPLGIAYFVIAVTGLSVSLGV
                     TGGALYSLITNDASHIQFDDVPWLQHLLHTAPGLLLLAFVGVLLFFVVLHIARAIGWL
                     HGRIAEVLLVRL"
     gene            complement(2123..2524)
                     /locus_tag="VG274_00930"
     CDS             complement(2123..2524)
                     /locus_tag="VG274_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWE05237.1"
                     /translation="MPPESDYTRKFRKELMAGLSSLVLLAVLARAGREMYGYEIAKAV
                     KAEGEGGLIFKQGAIYPVLRSLHATGLLESRVEASAFGPPRRYYNITDDGRRALAEWQ
                     FAWRDMREFVDDALKAGATGGGTGNEFRPSA"
BASE COUNT          535 a          790 c          854 g          550 t
ORIGIN      
        1 gctggccttc cggcgtataa tagtaattgt agaacacctc gaacgtgtcg cccggcttga
       61 tgtcgcgctg gaaatcgacc tcgtacgaga acatgtggat catctcgacc acgacgtcgg
      121 cgggaatgcc cgcgcgcatg gcgctgagat acaggctgcc ttcgatcgtg gcgccggcgc
      181 gatggatatg cgcctcgagc ttcttctcga catcgttcgc ggtgaacgcg ttgttcgcat
      241 cgcgcgtgat cgacacatca tgctcgaccg acggtgagaa attgatggag atcaggtgat
      301 tgacgggcgt cgtcgtcgcg gggctgatgg caccattcgc gttggcgtcg tcttcatcgc
      361 tgccatcgaa ggcgttattg cttgcctcgt tggcggcggc aacggcctga ggatcggtcg
      421 aatacgttac gtcgaaggat tggcccgcgc gcaggctgcg cgaattataa accctgccga
      481 gtgccgtgac ggccgcattg gcctcgtcgc tggaaacgcc cgcatcttcc agggcgccgg
      541 cgagcgtatc gccgcggtcc attgttattg tacgcgtctc gacgcccggc gccttgttct
      601 catcgtccgc ggcatcttgc gcggcgagat tgtcgtcaag tttggcaggc tgcgccgccg
      661 gcttccgcga gaccgatatg ccgaacggat tgatggaagc ctggttggtc gattgcgcga
      721 gcttctggaa cagttggtag ggtagaaacg ttgttgggtg gccggtgcca aagccgcgcg
      781 catgcatcgg cggcatcgtg ccgacggcca tccaggcgat tgaacccgcg atcaggcctg
      841 tgagggacgt cgtcagcaga gcccaacggc cttccgtcga ggaagccacg gttgctgctg
      901 agcgcgcgga aacgcgccag tcacttacag cagacatcaa agagaattgg ctgcgtcgct
      961 ccacatcggc tcctgagttt ctacggggac agttcttggc cccagcccaa agtagtcatg
     1021 cgtccggcga accggcagta accccttata gcgcaaagca tattaggcgc gtcaaccctt
     1081 accgggctgt taatggtgaa gcgtccgtgc gtgggattcc tgcctaagtg ccgggttcca
     1141 aagagttccg ccaaaaaaaa tagcgccgcg agtctttcga ttcgcggcgc gcaatgggcg
     1201 gctggggcag gcccggccat ggagggatta cagccggacg agaaggactt ctgcgatgcg
     1261 gccatggagc cagccgatgg cgcgggcgat gtgcagcacg acgaagaaca acagcacgcc
     1321 aacgaacgcg agcaggagaa gacctggcgc cgtgtgcagc agatgctgga gccacggcac
     1381 atcgtcgaac tggatgtgac tcgcatcgtt ggtgatcagg ctatagagcg ccccgccggt
     1441 gacaccgagc gacacggaaa gccccgtgac cgcgatcacg aagtacgcga tgccgagtgg
     1501 cagctttagc aggaaataga acatcgacga ccaggtgcgg ctatcgctca atccatcctt
     1561 gatgcgagtc cagatcgtct cgtcggattg cgtggtgggc ggcaggcggc gcggcatgcg
     1621 cacgccgagc aatccttcga cgatgcgacc ttccacgtgc gagagcacgc gtatcgagcc
     1681 gatgaacagc agcgcgaatg gaatgccgat gatcaggatg gcaaagccca gcgtcagcga
     1741 tatgccgacg acggtccagg taaagtagaa aatgcctgtc gcgagcgaca aaagcatata
     1801 cagcagcgcg ccataagtgc gcgggtcgga aacgacgttg aagaatccgt agcggcgctc
     1861 aggcgcgtca tcggatttgg ggaacggtcc gccaatggtg gcttccatgt ctctatactc
     1921 ctctgcgatt tcttgcggcg tgccgtaagt gtcgatcacg gtcgccagga tttgggattc
     1981 cggaatttcc gggttgtgcg aaatttcgtt gttgaggtgg tcttccgtgt cggcgagcgc
     2041 gtcggagatc agccccgcag gcgcgccttt cagcgcgcgc ctgagctcgt ccaagtaagc
     2101 cctgactgtc ttgggggccg tttcaggcgg acggtctgaa ttcattgccc gttcctccgc
     2161 cggtcgcgcc ggctttcaga gcgtcatcga cgaactctcg catgtcgcgc catgcgaact
     2221 gccactcggc cagcgcgcgg cgcccgtcgt cggtgatgtt gtagtagcgg cggggagggc
     2281 cgaacgccga agcctcgacg cggctctcca gcaaacccgt cgcatgtaac gaccgcagga
     2341 ccgggtagat tgcgccctgc ttgaagatca gcccgccttc gccttcggcc ttaaccgcct
     2401 tggcgatttc gtatccgtac atttcccgtc cggcgcgcgc gagcaccgcc agcaggacga
     2461 gcgatgaaag cccggccatg agttccttac ggaatttgcg cgtgtaatcg gattctggtg
     2521 gcacgacgcg ctactcccgc ctccctacta ttacgcactc acatctagca tggacttcgc
     2581 tatatgcaag actgccctgt agagaaattt gcaaaatttc tgctggagaa taggatatag
     2641 ttcatatcct ccgtgatttc cggcggcttt ccttgtcttt cagcggcaaa atctcgtcac
     2701 ggaattttga tgtgacggcc gaaatcctg
//
LOCUS       DASZMC010000048         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1406301,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000048 DASZMC010000000
VERSION     DASZMC010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406301"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VG274_00935"
     CDS             complement(<1..1171)
                     /locus_tag="VG274_00935"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05238.1"
                     /translation="MTMTPQQAKAYRSDPADAAIAAYDDERAAAAQVEGACSLLRPAS
                     PDTAAFCGALYGGASPEDVARYAPESLAALASLAYQRSRVRKTGQSLVDICDFHPHAG
                     DHTLNEAVFVAVNDDMPFLFDSLMGELAVQNVRAHVLLHPIMQVKRDAGGLRASDGTP
                     MRESVIVLVLDSVHDDAHQQALLEGARNVFAQVGLAVRDWRKMLAKLTDTIANLERRP
                     PKIPLEELRENIAFLEWLGDNHFTFLGCRDYAFDGAGGGRLIPVEESGLGLLSDSAAR
                     VIGRGSDHANLSAEARAFLDQPEPLIITKANERSVVHRRVHMDYVGVKTFDSTGSLSG
                     ERRFVGLFTSGAYSRRPADIPLLRLKIAHIIERAGLAPDSHDGKALAHILDTYPRE"
     gene            complement(1325..2101)
                     /locus_tag="VG274_00940"
     CDS             complement(1325..2101)
                     /locus_tag="VG274_00940"
                     /inference="COORDINATES: protein motif:HMM:NF027577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4908 domain-containing protein"
                     /protein_id="HWE05239.1"
                     /translation="MIRVIAERLAIAALLTGLACGSNAHGEDSVQERLSSDRLGRIET
                     GTYLAGEKIGFELDPAGDGYLLRFDDSPEVFVLHTDNASMGGRVLKYDSGETALQVSG
                     WGGITLYTDADPAGLPAVRTGDSVTPMPAATSIADVQNAAQDDAQHLAFVRRLNLAFG
                     ADWSSLAADAGARSLALEALENVARGLDRFGRSAQGHDALAHRVNTVMLAIAGRPTVT
                     LDGKKLMVTFDPGSGYEGCASSRAISRALVALLAARQKQN"
     gene            complement(2195..>2712)
                     /locus_tag="VG274_00945"
     CDS             complement(2195..>2712)
                     /locus_tag="VG274_00945"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWE05240.1"
                     /translation="TRMRLSKPVIAAVSGYAVAGGLELALWCDLRVAEEDAVFGVFCR
                     RWGVPLIDGGTVRLPRLIGQSRALDMILTGRPVGAGEAFSIGLANRVVPKGAARAAAE
                     ELARELARLPQLCLRSDRQSVYEQYNDDTAHALAREFALGSTTLRTGETLAGAARFAA
                     GRGRGGRFDDI"
BASE COUNT          514 a          855 c          866 g          477 t
ORIGIN      
        1 cttcgcgcgg gtaggtgtcg aggatgtgag cgagcgcttt gccgtcatga ctgtcgggcg
       61 cgagaccggc cctctcgatg atgtgggcga tcttgagacg gaggagcgga atatccgcgg
      121 gacgccggct gtaggcgccc gacgtgaaca atccgacaaa gcggcgctcc cccgagagcg
      181 aaccggtgga gtcgaacgtc ttgacgccca catagtccat gtgcacgcgg cgatggacga
      241 cggagcgctc gtttgccttt gtgatgatca aaggctcggg ctgatccaga aaggcgcgag
      301 cctcggcgga cagattcgcg tgatctgatc cacggccgat tacgcgcgcc gcggagtctg
      361 aaagaagtcc caggccgctc tcttcgactg gtataagccg tccgcctccg gcaccgtcga
      421 acgcataatc ccggcatccc aggaacgtga aatggttgtc gccgagccat tcgaggaacg
      481 cgatgttttc gcgaagttcc tcgagaggga tcttcggcgg ccttcgttcc agattggcga
      541 tggtatccgt caatttcgcg agcattttcc gccagtcccg caccgccagg ccgacctgtg
      601 caaacacatt gcgcgcgccc tccaggagcg cctgctggtg tgcatcgtcg tgaaccgaat
      661 ccagcaccag cacgatgacg ctttcgcgca tcggcgtgcc gtcggacgcg cgcaaaccgc
      721 ctgcatcccg tttgacctgc atgatcggat gtagcaggac gtgagcacgg acgttttgca
      781 cggccaattc gcccatcagc gaatcgaaga gaaacggcat gtcgtcgttt accgcgacaa
      841 acacggcttc attcagcgtg tgatcgcccg catggggatg aaaatcacaa atatctacga
      901 ggctctgccc tgttttgcgg acgcgggagc gctggtacgc caagctcgcc agagcggcga
      961 gcgattccgg cgcgtaccgc gccacgtctt cgggagacgc gccgccatag agtgcgccgc
     1021 agaacgcggc cgtatctgga gacgcgggcc tgagaaggga acatgcccct tctacctggg
     1081 cggcagcggc acgctcgtcg tcatacgcgg cgatcgccgc atcggcagga tcactgcggt
     1141 acgctttggc ctgttgagga gtcattgtca tccatgtgga agtgccggac ctgtaggcct
     1201 cgccgatcgg cgcgtggcag aatcatctcc gcgccgatat tgcatcagtc tccatactgg
     1261 cgggcggaat cttaacgccc tccgacgcgg aagggaatcc ggcgtttcgc cgaagaatgt
     1321 ccaatcaatt ctgtttttgc cgcgcggcca gcaaagcgac aagggcccgc gatattgcgc
     1381 gcgaggaggc gcagccttcg taaccgctgc cgggatcgaa ggtgaccatg agtttcttgc
     1441 catccagcgt cacggtagga cgcccggcga ttgcgagcat gaccgtgttc acgcggtgcg
     1501 ccagcgcgtc atgcccttgc gcagaccggc cgaaccgatc cagacctcga gcgacgtttt
     1561 ccagcgcttc cagggccaga gacctggcac ctgcgtcggc ggccaatgaa ctccagtcgg
     1621 cgccaaaggc cagattgagc ctccgcacga aggccagatg ctgggcatcg tcctgtgcgg
     1681 cgttctgcac gtcggcgatc gaggttgcag caggcatcgg cgtcacggaa tcgccggtgc
     1741 ggacggcggg caggcccgca gggtccgcgt cggtatagag agtgatgcct ccccagccgg
     1801 aaacctgcag cgcagtttcg ccggaatcgt acttcaatac gcggccgccc atcgaggcgt
     1861 tgtctgtgtg cagaacgaaa acttccggag agtcgtcgaa tcgaagaaga tagccgtcgc
     1921 ccgcggggtc gagctcgaag ccgatcttct cgccggccag atacgtcccg gtttcgatac
     1981 ggccgagccg atcggacgac agccgctcct ggacggagtc ttcgccatgc gcgttcgatc
     2041 cacaagccag tccggtcagg agcgcggcaa tcgctagacg ttcggcaata accctgatca
     2101 ttggcgcgga tcgctcgctc gccgcttcgc gactgcattg gccgccgccg aaacaatatg
     2161 tcggccgaat cgggcaaatt tgggaccgga cagactagat atcgtcgaag cggccaccgc
     2221 ggcccctgcc ggccgcaaaa cgtgcggccc cggcgagcgt ctcgccggtt cgcagcgttg
     2281 tggagccgag agcaaattcg cgggcaagcg catgtgcagt gtcgtcgtta tattgttcgt
     2341 atacggattg acggtcgctt cgcaggcaaa gttgcggcag gcgggcgagc tcgcgtgcca
     2401 gctcctccgc cgctgcgcgc gctgctcctt tcggcaccac gcggttcgcg aggccgatgg
     2461 agaaagcttc gccggctcca acgggccgtc cagtcaggat catgtcgagg gcgcgagact
     2521 ggccgatgag gcgcggaagg cggaccgtac cgccgtcgat cagcggcaca ccccagcgcc
     2581 ggcagaacac tccgaacact gcgtcttcct ccgcgacccg caaatcgcac cacaacgcca
     2641 gttcgaggcc gccggcgact gcgtagcccg agaccgctgc aataacgggc ttcgacaggc
     2701 gcatccgtgt gg
//
LOCUS       DASZMC010000049         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3868117,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000049 DASZMC010000000
VERSION     DASZMC010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3868117"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VG274_00950"
     CDS             <1..775
                     /locus_tag="VG274_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWE05241.1"
                     /translation="EDVIRRMWDNLGRTISEYAHLEKLSMKGARPRIEVTGLEHVESA
                     VASGKGIIFISAHFANWEVMPFAAVQHGLDGAEVYRPVNNPIVDRWMVRQRQTNGPTE
                     QIAKGAYGTRRLFTLLRAGKAIFMLVDQKTNEGVAAPFFGRDAMTTPAPAALALKLGA
                     VLLPVSNERLKGAHFRMTVHPAIAYEPSDDHARDVLALTTRINEKIEAIVRRNSAQWL
                     WIHRRWPKPGDMPRSRRGKEAQALAGTGVRAERDGSSLV"
     gene            complement(718..1557)
                     /locus_tag="VG274_00955"
     CDS             complement(718..1557)
                     /locus_tag="VG274_00955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWE05242.1"
                     /translation="MIARRAFLAASVALAGYPRAAFGAASRLALTGSFEQGSLVVGKT
                     EAGARIAIDGTMVHVSPAGNFAFGFSFDQKKPSVIAARFADETRETRIVEPVARQYEI
                     QRITGLPEKLVTLPPEVLERRKREVAMIAAAHAGDSDLDWFTQKFDWPVTGIISSTFG
                     RQRILNGKAMAPHFGVDIAARAGTPIAAPAPARVALAGPDFYLEGGLTILDHGHGVTT
                     SYLHQSRQTVKVGDNIARGQVIGEVGMTGRATGPHLHWGLNWFQTRLDPSRSARTPVP
                     ARA"
     gene            complement(1554..1781)
                     /locus_tag="VG274_00960"
     CDS             complement(1554..1781)
                     /locus_tag="VG274_00960"
                     /inference="COORDINATES: protein motif:HMM:NF021392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HWE05243.1"
                     /translation="MNQMERDFRPDGLADVEYLDGEYRIVKPGSFVLCAVTGERIPIE
                     ALRYWSVDLQEAYASPASALKRFQDRGLTPA"
     gene            complement(1846..>2325)
                     /locus_tag="VG274_00965"
     CDS             complement(1846..>2325)
                     /locus_tag="VG274_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWE05244.1"
                     /translation="ILPRTDQLLAPARQRFDLAAERLGSALNQNLREHRRLLIEAALL
                     LRPRGVTNRIEAGAERVRVLAHRLVRCQSLALRARRDRLDSLARLLDSVSHRSVLERG
                     FALVRGADGAIRRCAATVVAGEALSLVFADSTIHAKAQGRSARSSAPRRKTGQADLF"
BASE COUNT          451 a          735 c          720 g          419 t
ORIGIN      
        1 tgaagacgtt atccgtcgaa tgtgggacaa tctcgggcgc acaatttcgg aatatgcgca
       61 tctcgaaaag ctgtcgatga agggtgctcg cccgcgcata gaagtcacgg ggctcgaaca
      121 tgtcgagagc gcggtcgcga gcggaaaggg aatcatcttc atctcggcac acttcgccaa
      181 ttgggaggta atgccgttcg ccgccgtcca acatggcctg gacggagccg aagtctatcg
      241 gcccgtcaac aatccgatcg tcgatcgctg gatggtgcgc cagcgccaga ccaacggccc
      301 cacggagcaa atcgcgaaag gtgcgtacgg tacaaggcgt cttttcacgc ttcttcgtgc
      361 aggcaaggcg attttcatgc tcgtcgacca gaagacgaac gagggagtcg ccgcgccgtt
      421 cttcggccgc gacgcaatga ccactccggc tcccgccgcg cttgcgctca aactgggcgc
      481 cgttctgctg ccggtatcaa acgaacgcct taagggcgcg cattttcgta tgacggtaca
      541 tcccgcaatt gcctatgaac cttccgacga tcatgcccgt gacgtgctcg cactgacgac
      601 aaggatcaac gaaaaaattg aagcgatcgt gcgccgaaat tcggcgcaat ggctatggat
      661 ccatcgccgt tggcctaaac cgggagacat gccgcgctcg cggcgtggaa aagaggctca
      721 ggcccttgcg ggcacgggcg ttcgcgccga gcgcgacgga tcgagccttg tctgaaacca
      781 gttgagtccc cagtgaagat gcgggccggt cgcgcgtccg gtcatgccca cctctccaat
      841 tacctgtccg cgcgcgatgt tgtcgccaac cttcacggtc tgcctgctct ggtgcagata
      901 ggatgtggtc accccgtggc cgtggtccag aattgtcaga ccgccttcga ggtagaaatc
      961 cggcccggcg agagcaacgc gcgccggagc cggggccgct ataggggtgc ccgcccgcgc
     1021 cgcaatatcg acaccgaaat gcggcgccat cgccttgcca ttgagtattc tctgcctgcc
     1081 aaatgtgctg ctgatgatgc cggtcaccgg ccaatcgaac ttttgcgtga accagtccag
     1141 atcgctgtcg ccggcatgcg cggcggcgat catcgccacc tcgcgctttc ggcgctccag
     1201 cacttccggc ggcagcgtta cgagcttttc cggcaggccc gtgatccgtt gaatttcgta
     1261 ttgacgagcg acaggctcaa cgattcgcgt ctcgcgcgtt tcgtcggcga agcgtgcggc
     1321 gatcacgctg ggctttttct ggtcgaagga gaatccgaag gcaaagttgc ccgcaggaga
     1381 gacgtgaacc atcgtgccgt cgatcgcaat gcgcgcgccc gcctcggttt tgccgacaac
     1441 gaggctgcct tgctcgaacg aaccggttag cgcaaggcgg gaggccgcgc cgaacgcagc
     1501 gcgcggatac ccggccagtg caaccgacgc agcgagaaac gcacgccgcg cgatcatgcg
     1561 ggtgtgaggc cgcgatcctg gaagcgtttg agagcgctcg cggggcttgc gtacgcttcc
     1621 tgcagatcga cgctccaata gcgtaacgcc tcgatcggaa ttcgttcgcc cgtcaccgcg
     1681 cacaggacga acgagcccgg cttgacgatc cggtactcac cgtcgagata ctcgacgtcg
     1741 gcaagcccgt ccggccgaaa atcccgttcc atctggttca tgcctgaaac tttacgccga
     1801 tatgcgcgtc gaaaacaaat tgcttgaagg aacgcttccc gctgctcaaa ataaatcggc
     1861 ttggcccgtc ttgcggcgcg gtgcgcttga gcgtgctgac cgaccctggg cctttgcatg
     1921 aattgtcgaa tcggcaaaga cgagcgaaag cgcttcgccg gcgacgaccg tggcagcgca
     1981 ccgccggatc gctccatccg ccccgcggac cagtgcaaag ccgcgttcga gcacggagcg
     2041 gtggctcacg ctgtcgagaa gccgcgccaa tgaatcgagc ctgtcgcgcc gcgcccgcag
     2101 agcgaggctc tgacagcgga cgagccggtg cgcgagcaca cgtacgcgct ccgctccggc
     2161 ctcgatacga ttcgtgacgc cgcgcggccg gagcaatagg gctgcctcga tcaggagccg
     2221 gcgatgctcc cggagatttt ggttaagtgc gcttccgagc ctttccgcag caaggtcgaa
     2281 acgctgccgc gccggcgcca ggagctggtc ggtccgcggc agaat
//
LOCUS       DASZMC010000050         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4692481,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000050 DASZMC010000000
VERSION     DASZMC010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4692481"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VG274_00970"
     CDS             complement(<1..714)
                     /locus_tag="VG274_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE05245.1"
                     /translation="MTSPVFELRDVRYSYQSHVAIDGLSLAMPSGQRVALLGANGSGK
                     STLLRLLDALYFPESGSILFHGETLTAERFDNDRFAFDFRRRVGLVFQNPDVQLFNPT
                     VFDEVAFAPLQLRWSKPEIRARVYETLEQMEILHLRDRPPHRLSGGEKKRVALASVLI
                     LDPEVLLLDEPTAALDPRSQSQIIDLLIGWGGGGKTVITATHDLGLVEDIADYCFILK
                     QGKIAAAGEPRDLLQNEQLL"
     gene            complement(711..1520)
                     /gene="cbiQ"
                     /locus_tag="VG274_00975"
     CDS             complement(711..1520)
                     /gene="cbiQ"
                     /locus_tag="VG274_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HWE05246.1"
                     /translation="MRRGFIESTIAGLLTASEYAAAAENLASERGVLQRIDPRVKVAG
                     LFTLIVIAAASRSLALIGAVFAIALVLAAGSRIGPLRLARLVWLPVLFFTGAIAFPAI
                     FLTPGTRLTAVAGLHITHQGLRAASFLIARAETAATLSALLALTTPWPWVLKALRSLG
                     CPLVLVAIFGMTFRYIFVILQTAAEMFESRQSRTVGELKGSDRRRLAASAAGVLLSKS
                     LQLSGDVHLAMQSRGFRGEVYIVHDFRTRLADWLWLSFFIAVAAAAIRFGK"
     gene            complement(1517..2569)
                     /gene="cbiM"
                     /locus_tag="VG274_00980"
     CDS             complement(1517..2569)
                     /gene="cbiM"
                     /locus_tag="VG274_00980"
                     /inference="COORDINATES: protein motif:HMM:NF008873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HWE05247.1"
                     /translation="MHIPDGYLGPSTCLALFGGAAPFWWISLRRMKRALHTRTVPLLS
                     LFSAFSFIVMMFNLPLPGGTTGHAVGMGIASIVLGPWAAILAISIALTIQAVFFGDGG
                     ITAIGANCFNMAIVGSLVSYCLYRAVSGRAAIRSSRRVIAAGIAGYTAINVSALLAAI
                     EFGIQPALYHDASGAPLYAPYPLHIAIPAMTIGHLTVAGLAEMVISAGVVGYLQRAEP
                     GLLKNTAPGAVLETEALPPATARPLWIALALLMILTPLGILAAGAAWGEWAPREFVTA
                     PQKIAAASGNHPLPAAVPSGLQRLSSFWTAPMPGYAPPFLKNPSFGYLLSAVAGTGIV
                     LLVCALAGFFTRGSAP"
BASE COUNT          565 a          706 c         1054 g          403 t
ORIGIN      
        1 caggagttgc tcgttttgaa gtagatcgcg gggctcgcct gcggctgcga ttttgccctg
       61 cttcaggatg aagcagtagt cggcgatgtc ttcaacgaga cccaggtcgt gggtggcggt
      121 gatgacggtt ttacctcctc cgccccagcc gatgagcagg tcgatgatct ggctctggct
      181 gcgtggatcg agcgcggcgg tgggctcgtc gagcaggaga acttcgggat cgaggatgag
      241 gacggaagcg agggcgaccc gtttcttttc gcctcccgag aggcgatgcg gcgggcggtc
      301 gcgaaggtgc agaatctcca tttgttcgag tgtttcgtag acgcgggcgc ggatctcggg
      361 tttggaccag cggagttgca ggggcgcgaa ggcgacctcg tcgaagacgg tgggattgaa
      421 gagttgcacg tcgggattct ggaagacaag gccgacgcgg cggcggaaat cgaaggcgaa
      481 acgatcgtta tcgaagcgct cggcggtcag cgtttcgccg tggaacagaa tgctgccgga
      541 ttcggggaaa tagagggcgt cgagaaggcg gagcagggtg gatttgccgg agccgttggc
      601 gcccagcaag gctacgcgct ggccggacgg catggcgagc gagaggccat ctatcgcgac
      661 atgcgactga tacgaatagc ggacgtcgcg caattcgaag acaggcgacg tcatttaccg
      721 aaccggatgg cggcagccgc cacggcgatg aagaatgaga gccagagcca gtcggccaga
      781 cgcgtgcgga agtcatgaac gatgtagact tcgccgcgaa atccgcgtga ctgcatggcg
      841 aggtggacgt cgccgctgag ttgcaggctt ttactgagca gcacgcccgc ggcggacgcg
      901 gcgagacggc ggcgatcgga acctttgagc tctccgacgg tgcggctctg gcgcgattcg
      961 aacatctcgg cggcggtctg caggatgaca aaaatatagc ggaaggtcat gccgaagatg
     1021 gcgacgagga ccagcgggca tccgagactc cggagggctt tgaggaccca gggccagggc
     1081 gtggtgagcg ccagcagggc ggacagtgtg gcggcggttt cggcgcgggc aatcagaaac
     1141 gacgcggcgc gcagaccctg atgggtgatg tggaggccgg ctacggcggt gaggcgcgtg
     1201 cccggcgtga ggaaaatggc ggggaacgcg atggctccgg tgaagaagag aaccggcagc
     1261 cagacgaggc gcgcgagccg cagcggtccg atgcgcgatc cggcggcgag gaccagcgcg
     1321 atcgcgaaga ccgcgccaat gagggccagg ctgcgcgatg ccgccgcgat gacgatcagc
     1381 gtgaacaggc cggccacttt gacgcgggga tcgatgcgct ggagcacgcc gcgctccgag
     1441 gcaagatttt cggcggcggc ggcgtattcg gaagcggtga ggagtccggc gatggtcgat
     1501 tcgatgaagc cgcgtctcat ggcgcggacc cacgggtgaa gaagccggcg agcgcgcaca
     1561 cgagcagaac gataccggtt cccgcgacgg ctgagagcag atagccgaag gaaggattct
     1621 tcaggaaggg cggggcgtag ccgggcatgg gcgcggtcca aaacgaggag aggcgctgga
     1681 ggccggaagg aactgccgcg ggcaaaggat gattaccgga ggccgccgcg atcttctgag
     1741 gcgctgtgac gaattccctc ggggcccatt cgccccatgc ggcgccggcc gcgagtatac
     1801 ccagcggtgt gaggatcatc aggagggcga gcgcgatcca gagcggtctg gcggttgcgg
     1861 ggggaagcgc ttcggtttcg agcacggcgc cgggtgcggt gtttttcagc agccccggtt
     1921 cggcgcgctg cagatagccg acaacgcccg ccgagatcac catttcggcc agtccggcca
     1981 ctgtgagatg gccgatcgtc atggcgggaa tggcgatgtg cagcgggtaa ggcgcataga
     2041 gcggggcgcc cgatgcatcg tgataaaggg ccggctgaat gccgaactcg atggcggcga
     2101 gcagcgcgga gacgttgatg gccgtgtatc ccgcgatgcc ggcggcgatg acgcggcgtg
     2161 aagaacgaat cgcggcgcgg cccgagacgg cgcgataaag acagtaagaa accagcgaac
     2221 cgacaatcgc catgttgaag cagttggcgc cgatggcggt gattccgccg tcaccgaaaa
     2281 agacggcctg aatggtaagc gcgatggaga tggcgagaat ggcggcccac ggtccgagca
     2341 cgatggaggc gatgcccatg ccgacggcgt gtccggtggt gcctccgggc agcggcaggt
     2401 tgaacatcat cacgatgaag gaaaacgcgg agaaaagaga gaggagcggg acggttcggg
     2461 tgtggagcgc gcgtttcatg cggcggaggg agatccacca gaaaggtgcg gctccgccga
     2521 acagcgcgag gcaggtggag ggtccgagat agccgtccgg gatgtgcata tcagggcagg
     2581 ctacagggca gaggattcgc gatgaataag ggaaccatgc gggtactgat gttcccacga
     2641 tatcgcgaag gaaacgtgcc gcgcggatgg cggcggggga ggggcgtggg gagaggaaat
     2701 cgggaataaa aacagggccc ggaatcga
//
LOCUS       DASZMC010000051         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1119712,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000051 DASZMC010000000
VERSION     DASZMC010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119712"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VG274_00985"
     CDS             <1..222
                     /locus_tag="VG274_00985"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWE05248.1"
                     /translation="PYRRIRHPLYLAGLVGSLGVMLQYRQPWALLVVLATFALQIVRI
                     NYEEQVLSRAFPEYAAYASRSWRLIPGVY"
     gene            364..1164
                     /locus_tag="VG274_00990"
     CDS             364..1164
                     /locus_tag="VG274_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWE05249.1"
                     /translation="MKTRCIVLAGAVLLAVAGPAAAGEGFYIGLGAGWDGQNNIKVDQ
                     LTPPPGSGEVQTNDGVVFAGTLGFKLPEFPIRLEFESGYDWHSVANVQSGNAQFGATG
                     HANIASELVNAVYVFPVAPGWNIYGGAGVGEGHVWFAPNFADTGEEIAHVDHWGFMWQ
                     GIAGTSFEIAPDVDLFADYRYRHVSANVESFSPALGPVATHDITENVVMGGVRFYMFP
                     PMEGPPPPPYEPPPQPQYQQQQNTMPPPPPPPAGSGGATSGGAGGGPQ"
     gene            1633..>3267
                     /locus_tag="VG274_00995"
     CDS             1633..>3267
                     /locus_tag="VG274_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWE05250.1"
                     /translation="MPVYRAPLPDYRFLLHELFEVEKQTDLPQCSEMNADVVDDVLSG
                     AGKFCEEVLQPLNQSGDEEGCHFENGVVRTPKGFRGAYQEYARAGWSGLGAPAEFGGS
                     NLPLFLTLLVGEMSQSANQSFAMYPALSMAAYSALIAAGAPWMKEHIVPKMVAGEWTG
                     TMCLTEPNCGTDLRLMKTRAVEQDDGTYRMNGTKIFISGGEHDLADNIIHMVIAKIPD
                     ADGQIHDDLATVNFFMVPKFVVKEDGTTGARNGVFCGGIEHKMGIRANATCVLNFDDA
                     IAWRLGPKPAPAKPGEKRSASEGMKGMFAMMNAARLGVGVQGISIAEVAQQNALAYAL
                     ERRAGHALTGPKEPGKPADLLIVHPDIRRMLLHCRSFVEGARAVAVWTALNMSIAHSA
                     RPERDTAGLLADLMTPVIKAFFTDMGFEAANMAMQCYGGHGYIRDNGVEQFVRDGRIN
                     QTYEGANGVQAMDLVGRKLGRKGGAAPIALFSMITGFVKDNGGDEQLAPFVRAVQRGL
                     ENLQSATMWLAQHGAANPNDAGAGATDYLRLTGIVVV"
BASE COUNT          590 a         1050 c         1050 g          577 t
ORIGIN      
        1 ccctatcgcc gcatccgcca ccctctttac ctcgctggcc tcgtcggatc gctcggcgtg
       61 atgctgcaat accggcagcc ctgggcgctg ctcgtggtgc tcgccacgtt cgcgctgcag
      121 atcgtgcgca tcaactacga ggagcaggtg ctctcccgcg ccttccccga atatgcagcc
      181 tatgcctcgc gaagctggcg gctcattccc ggcgtgtact gaatcactcg accagcttgt
      241 taaccgccaa gccgccaagg gccagtgtag gggattgtcc gacagcgcat ttcgcaggcg
      301 cgggatacat tgtcgaatcc gcgcgttgaa gcgctggcaa cgggatgaag aggcgggttt
      361 cacatgaaaa cgcgatgcat tgtattggcc ggcgcagtgc tgcttgccgt ggcgggtccg
      421 gctgccgcgg gcgagggttt ctacatcgga ctgggcgccg gctgggacgg ccagaacaac
      481 atcaaggtgg accagctcac cccgcccccc ggctccggcg aggttcagac caatgacggc
      541 gttgtcttcg ccggcacgct cggattcaag ctgcccgaat ttccgatccg gctcgaattc
      601 gagtccggct atgactggca cagcgtcgcc aacgtgcaga gcggcaatgc ccagttcggc
      661 gccacgggac atgccaacat cgcctccgag ctggtgaacg cggtctacgt ttttcccgtc
      721 gcgccgggct ggaatatcta tggcggcgcg ggagtgggcg aaggccatgt ctggttcgcg
      781 cccaacttcg ccgacacggg cgaggagatc gcccatgtcg atcattgggg attcatgtgg
      841 cagggaatcg ccggcacgtc gttcgaaatt gcgcccgacg tggatttgtt cgccgattac
      901 cgctatcgcc atgtgagcgc caatgtcgaa agcttctcgc ccgcgctcgg cccggtggcg
      961 acacatgaca tcaccgagaa cgtggtgatg gggggcgtgc gcttctacat gttcccgccg
     1021 atggaaggcc cgccgccgcc cccttacgag ccgccgcccc aaccccaata tcagcaacag
     1081 cagaacacaa tgccgcctcc gccgcccccg ccagcaggtt cgggcggcgc cacctccggc
     1141 ggcgcgggcg gaggcccaca gtaggtttca attcccgggg acagcatcgc tgtccccggc
     1201 ctccgggtgc gtgcgccgtt accgcaaacg cggcaggcgc gcggttgcct agctgtagat
     1261 tgctgtcagt gatcgcaggg aggatttact ggccagcatc acaatccgca aacttgatgt
     1321 cgcgctcgag cggagactgc gtgtgcgcgc ggcgcagaac gacacgtcga tggaagaaaa
     1381 accgcgcgat attcgtcgca gtgcgctggc ggcgagccgg cgctcgccgc gaatctggcc
     1441 atccgggcgc gcaccgggcc tctgggcggg gtagatctca ggcttccccg gcgcaggcgc
     1501 atgtcccgct cgcaatcgcg caaatagagt gcgagttccg tcatccaagc atcgatcgcg
     1561 aacgccccac ctcaatcgac accaagcatc cttcccgcct atacagtttc gcctcgtccc
     1621 ggagccttct ccatgcccgt ctatcgcgcg ccgcttccag actatcgctt tctcctgcac
     1681 gagctgttcg aggttgagaa gcagacggac ctgccgcaat gcagtgagat gaatgcggat
     1741 gtcgtcgacg atgtcctctc cggtgcgggc aagttctgcg aggaagttct gcagccgctg
     1801 aaccagtcgg gcgacgagga gggctgccat ttcgagaacg gcgtcgtgcg cacgccgaag
     1861 ggtttccggg gtgcgtacca ggaatatgcg cgagccggct ggagcgggct cggcgccccg
     1921 gcggaattcg gcggctcgaa tctgccgctt tttctcacgc tgctggtggg cgagatgtcg
     1981 caatcggcga accagtcttt tgcgatgtat ccggctcttt caatggcggc ctattcggcg
     2041 ctgatcgccg ccggcgcgcc gtggatgaag gaacacatcg tgccgaagat ggtggcgggc
     2101 gaatggacgg gtacgatgtg cctcaccgag cccaattgcg gcaccgatct caggctgatg
     2161 aagacgcgcg ccgtcgagca ggatgacggc acctaccgca tgaacgggac gaagattttc
     2221 atctccggcg gcgagcacga tctcgccgac aacatcattc atatggtgat cgccaagatt
     2281 cccgatgcgg acggacagat tcacgacgat ctcgcgacgg tcaatttctt catggtgccg
     2341 aaattcgtgg tgaaggagga cggcacgacg ggcgcgcgca acggcgtctt ctgcggcggc
     2401 atcgaacaca agatgggcat cagggcgaac gccacctgcg tgctcaattt cgacgacgcc
     2461 atcgcctggc ggctggggcc gaaacccgcg ccggccaagc ccggcgaaaa acgttcggcg
     2521 tccgaaggca tgaagggcat gttcgcgatg atgaacgccg cgcggctcgg cgtgggcgtg
     2581 cagggaatct ccatcgccga agtcgcccag cagaacgcgc tcgcctatgc gctcgagcgc
     2641 cgcgccggcc atgcgctcac cggaccaaag gaacccggca agcccgcgga ccttctcatc
     2701 gtgcatcccg acatccgccg catgctgctc cattgccgct ctttcgtcga aggcgcgcgc
     2761 gcggtcgcgg tgtggacggc gctcaacatg tcgatcgccc attccgcgcg gcccgaacgc
     2821 gacaccgctg gtttgctcgc cgatctgatg acgccggtga tcaaggcctt cttcaccgat
     2881 atgggcttcg aagcagcgaa tatggcgatg cagtgttacg gcggccatgg ctatatccgc
     2941 gacaatggcg tcgagcagtt cgtgcgcgac gggcgcatca accagacata cgaaggcgcc
     3001 aacggcgtgc aggcgatgga tctcgtcggc cgcaaactcg gccgcaaggg cggtgcggcg
     3061 ccgatcgcgc tgttctcgat gatcaccggt ttcgtgaaag acaatggcgg cgacgagcag
     3121 ctcgcgccgt tcgtgagggc ggtgcagcgc ggtctcgaga acctgcagag cgcgacgatg
     3181 tggctggcgc agcacggcgc cgccaatccc aacgatgccg gcgcaggcgc gaccgactat
     3241 ctgcgcctga cgggaatcgt cgtggtc
//
LOCUS       DASZMC010000052         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4149735,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000052 DASZMC010000000
VERSION     DASZMC010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4149735"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VG274_01000"
     CDS             <1..1521
                     /locus_tag="VG274_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWE05251.1"
                     /translation="NLCVVGDDDQSIYGWRGAEVENILRFERDFPGAKVIRLERNYRS
                     TPAILGAASGLIAANKGRLGKTLWTEGDQGEKIRVQGVWDAEEEARNIASDAEDLHRQ
                     NNAFSQMAILVRASFQMREFEDRFISLGLPYRVIGGPRFYERAEIRDALAYLRLVAQG
                     DDDLAFERIVNKPKRGIGDASVQSLHKFARAQRLPLLAASAEIAATDELPPKARKALA
                     ELAANFARWSETAKHLPHTEVAEMVLDESGYTDMLKADKSAEAPGRLENLKEFVRSME
                     SFESLSAFLEHVSLVMEIAQDESGDRINLMTLHAAKGLEFDTVFLPGWEEGLFPSQRT
                     MDENGLAGLEEERRLAYVGLTRAKKRIRVSFAANRRVHGSWQSALPSRFITELPQDHV
                     DMVVDEGFYGGNAGFRDNANAQEFASTYDSPGWRRAQANRAAQGSMRTRPPMIDGQAY
                     AVQTSDPSATQFARGERIFHQKFGYGIINAVEGNKLTVDFDKAGEKKVIDQFVQRA"
     gene            complement(1522..>1957)
                     /locus_tag="VG274_01005"
     CDS             complement(1522..>1957)
                     /locus_tag="VG274_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05252.1"
                     /translation="KRTQTWFYSWMRSFLLLTMLVLISTRAMADYIPPCTPPAGVIVF
                     TNLKNISKPLAEVLDHDFGYIAAPGEDFDATDVATTGHFIRLIFVWKRRDTWIVALER
                     GGIGYNDPIRKYLAQRDKVKLVTEELAIPETVCSVAHTVIKK"
BASE COUNT          426 a          592 c          597 g          342 t
ORIGIN      
        1 aatctctgcg tggtgggcga tgacgaccag agcatttatg gctggcgcgg cgcggaggtg
       61 gagaacatcc tgcgctttga gcgcgatttc cccggcgcga aagtgatccg ccttgagcgc
      121 aactatcgct ccacgcccgc aatcctcggt gcagcatccg gcctgatcgc ggcgaacaag
      181 ggccgcctcg gcaagacgct gtggaccgag ggcgatcaag gcgaaaaaat ccgtgtgcag
      241 ggtgtgtggg acgcggaaga agaagcgcgc aacatcgcca gcgacgcgga agacctgcac
      301 cgtcagaaca atgctttctc gcagatggcg atcctcgtgc gcgcgtcgtt ccagatgcgc
      361 gagttcgaag accggttcat ttcgctcggc ctgccctatc gcgtgatcgg cggcccgcgt
      421 ttttatgagc gtgcggaaat ccgcgatgcg ctcgcctatc tgcgcctcgt cgcgcagggc
      481 gatgacgacc tcgcgttcga gcgtatcgtc aacaagccca agcgcggcat cggcgatgcg
      541 agcgtgcaaa gcctgcacaa attcgcgcgg gcgcagcgcc tgccgctgct cgcggcatcg
      601 gctgagatcg ccgcgaccga cgagctgccg ccgaaggcgc gcaaggcgct ggccgagtta
      661 gccgcgaatt tcgcgcgctg gagcgagacc gcgaagcacc tgccccacac cgaagtggcg
      721 gagatggtgc tggacgaaag cggctacacc gacatgctga aggccgacaa atcggcggag
      781 gcgccggggc ggctggagaa cctgaaggag ttcgtgcgct cgatggaatc gttcgagtcg
      841 ctgtccgcgt ttctcgaaca tgtcagcctc gtgatggaga tcgcgcagga cgaaagcggc
      901 gaccgcatca atctgatgac gctccacgcc gccaaaggtt tggaattcga caccgtgttc
      961 ctgcccggct gggaggaagg cctgttcccg tcacaacgca cgatggacga gaacggactc
     1021 gcgggcctcg aggaggaacg ccgcctcgcc tatgtcgggc tcacgcgcgc gaagaagcgc
     1081 atccgcgtgt cgttcgccgc caaccggcgc gtgcatggca gctggcagag cgcactgccc
     1141 tcgcgcttca tcaccgagtt gccgcaggat cacgtcgaca tggtcgtgga cgaaggcttc
     1201 tacggcggca acgccggctt ccgcgacaac gccaacgcgc aggaattcgc gagcacctat
     1261 gacagccccg gctggcgccg cgcgcaagcc aaccgcgccg cgcaaggcag catgcgcaca
     1321 cgcccaccca tgatcgacgg ccaggcctat gccgtgcaga ccagcgatcc ctccgccacg
     1381 caattcgccc gcggcgaacg catcttccac cagaaattcg gctacggaat catcaacgcc
     1441 gtcgaaggca acaaactcac cgtcgatttc gacaaggcgg gcgagaaaaa ggtgatcgat
     1501 cagttcgtgc agagggcgtg attacttctt aattactgtg tgagcgacag aacaaactgt
     1561 ctcggggata gccagttctt cagtcaccaa tttcacctta tcgcgttggg caaggtattt
     1621 tcgtataggg tcattgtacc caatcccgcc gcgctcaagc gcgacaatcc aggtatcacg
     1681 tcgcttccaa acaaatataa gccgaatgaa atggcctgtg gtggcaacat ctgttgcgtc
     1741 gaagtcctcg ccgggcgcag caatgtagcc gaaatcgtgg tcaagcactt ctgcgagagg
     1801 ctttgaaatg ttcttcaaat tagtgaaaac aatgacgcct gcgggcggag tacatggcgg
     1861 aatataatcg gccattgccc gcgtcgagat taagaccagc attgtgagca aaagaaaact
     1921 tctcatccat gaatagaacc atgtttgagt ccgctta
//
LOCUS       DASZMC010000053         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1407514,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000053 DASZMC010000000
VERSION     DASZMC010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407514"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VG274_01010"
     CDS             complement(<1..391)
                     /locus_tag="VG274_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWE05253.1"
                     /translation="MAQAGVESISTPSRDDFAAMLEASFETRAPAEGAVVRGVVVAIE
                     NDFAIVDVGLKTEGRVALKEFSMPGQPPNISLGSEVEVYVERIENALGEAVLSRDKAR
                     REESWIRLEKAFNDQTQVVGVIFGRVKG"
     gene            complement(542..1210)
                     /gene="cmk"
                     /locus_tag="VG274_01015"
     CDS             complement(542..1210)
                     /gene="cmk"
                     /locus_tag="VG274_01015"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWE05254.1"
                     /translation="MTCPIVIAVDGTAASGKGTLARRLAAHFHFAHLDSGSLYRLTAL
                     RILRAHGDLRDEAAAVASARSVDPADAGRYDLRGREIAEGASVVAAIPGVRAELLAWQ
                     QRFARNPGAAGAVIDGRDIGTVICPDATAKLFVDAAPELRARRRRLELEAAGEPASEA
                     AILAEIRARDARDRNRAVAPLLRAPDAALLDTSDLDIDAAFAAALALVEPRIEEALAA
                     RQRG"
     gene            complement(1207..2559)
                     /gene="aroA"
                     /locus_tag="VG274_01020"
     CDS             complement(1207..2559)
                     /gene="aroA"
                     /locus_tag="VG274_01020"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWE05255.1"
                     /translation="MKAPEISRPLEARRSGPLKGVALVPGDKSISHRALILGALAVGE
                     TKISGLLEADDVLCTAAGVRKLGVTVRQEGDGEWRVHGVGTGGFAEPDDVLDFGNSGT
                     GARLVMGAIATTPITAAFTGDASLRKRPMHRVLDPLARFGATYAARAGGHMPLSLTGA
                     RRPIAIEHHLEIASAQVKSALLLAALNAPGLSRISQSALTRDHTERMLTAFGAEIGIE
                     MLPGGGEAVHVMGEAELHPAMVRVPRDPSSAAFPLAAALLVEGSEVTLPGVLVSPRRI
                     GLLETLREMGAAIEIVNNDESGGEAIADLRVRACPLKGVEVPPMRAPSMIDEYPILAV
                     VAAFAEGRTIMRGLEELRVKESDRIAGIASGLAAIGVAVEELPDGLIVEGRGTDGVEG
                     GACIAANMDHRLAMSFLVAGLATRRPVSIDDSTMVRTSFPKFRPLMRSLGADLSVPGR
                     "
BASE COUNT          478 a          952 c          890 g          453 t
ORIGIN      
        1 cgcctttcac gcggccgaag atgacgccga cgacctgcgt ctggtcgttg aaggccttct
       61 ccaggcgaat ccagctttcc tcgcgccgcg ccttgtcgcg cgacagcacc gcttcgccga
      121 gcgcattttc aatgcgctcg acatagactt cgacctcgct gcccaggctg atattgggcg
      181 gctggccggg catcgagaat tccttcaagg cgacgcggcc ctcagtcttc agtcccacat
      241 cgacaatggc gaaatcgttc tcgatggcga cgacgacgcc gcgaacgacc gcgccctcgg
      301 caggcgcgcg ggtttcaaag ctggcttcca gcatcgccgc gaaatcgtcg cgactggggg
      361 ttgaaatgga ttcgacgcca gcttgtgcca tgatgtgtcc ttctcgataa cgggtttcgc
      421 ctggccggat cggcctgccg tcctgtgcga gccaccgcaa cagccatggg cgtcgcgaac
      481 gcgcgcgtga aaactttggg agtgcttcgc aagcctgccg atgcgagccc gccgggtctc
      541 cttaacccct ctggcgggct gcgagcgctt cctcgatcct gggctcgacc agggcgaggg
      601 ctgccgcgaa cgcggcgtct atatccaaat cgctcgtatc aagcaaggcc gcgtccggag
      661 cgcgcagcag gggcgcgaca gcgcggttcc ggtcgcgggc gtcgcgcgcc cttatctcgg
      721 cgagaatggc agcttccgaa gccggctcgc cggccgcttc cagttcaagc cggcggcggc
      781 gggcgcgaag ttcgggggct gcatcgacga agagcttggc ggtggcgtcg gggcaaatga
      841 ccgtgccgat gtcgcgtccg tcgatcaccg cgcccgcggc gccgggattg cgcgcaaacc
      901 gctgctgcca cgccagcaac tccgcgcgca cgccggggat cgccgccacc actgatgcgc
      961 cctcggcaat ttcgcgcccg cgcaaatcgt agcgccccgc atcggccgga tcgacgctgc
     1021 gtgccgacgc aaccgccgca gcctcgtcgc ggagatcgcc atgcgcgcgc agaatgcgca
     1081 gcgcggtcaa gcgatacagc gagcccgaat cgagatgggc gaaatggaaa tgcgcggcga
     1141 gacgcctggc cagggttccc ttgccggagg ctgccgtgcc gtccacggcg atcacgatgg
     1201 ggcaggtcat cgtccgggca cggacaaatc ggcgccgagg ctgcgcatga gcggcctgaa
     1261 tttgggaaag ctcgtgcgca ccatcgtgga atcgtcgatc gacaccggcc ggcgcgtcgc
     1321 aagtcccgcc acgaggaacg acatcgcaag gcgatgatcc atgttggcgg cgatgcatgc
     1381 cccgccttcg acgccgtccg ttcccctgcc ctcgacaatc aatccgtcgg gaagttcctc
     1441 gacagcaacg ccgatcgccg cgaggccgga cgcaatgccg gcaatgcgat cactttcctt
     1501 gacgcgcaat tcttcgagtc cgcgcatgat ggtacgcccc tctgcaaacg cggcgacgac
     1561 tgcgaggatg ggatactcgt cgatcatcga cggcgcacgc atgggcggca cctcgacgcc
     1621 cttcagcgga caggcgcgga cgcgaagatc ggcgatcgcc tcgccgccgc tttcatcgtt
     1681 gtttacgatc tcgattgcgg cgcccatctc ccgcagtgtc tcgagcaagc cgatacgcct
     1741 gggactgacg agcacgccgg gaagcgtcac ctcggatcct tccacgagca aggcggcggc
     1801 gagcggaaat gcggcggacg agggatcgcg cggcacgcgc accatcgcgg gatgcagctc
     1861 cgcttcgccc atgacatgaa cggcttcccc gccgccggga agcatctcga tgccgatctc
     1921 tgcgccgaag gccgtcagca tgcgctcggt atgatcgcgc gtgagcgcgc tctgggagat
     1981 gcgtgagagt ccgggcgcat tgagcgcagc gagcaacaat gccgatttca cctgcgcgga
     2041 ggcgatttcg aggtggtgct cgattgcgat cgggcgccga gcgccggtga gcgacagcgg
     2101 catgtggcct ccggcccgcg ccgcgtaggt ggcgccgaaa cgtgcgagcg gatcgagcac
     2161 gcgatgcatc ggccttttcc tgagcgatgc atcgccggtg aacgccgccg taatcggcgt
     2221 cgtggcgatc gcacccatga caaggcgcgc gcctgttccg gaattgccga aatccagcac
     2281 gtcgtccggc tccgcgaacc cgcccgtgcc tacgccgtgt acgcgccatt cgccgtcccc
     2341 ctcctgacgc acggtgacgc cgagtttgcg caccccggcg gccgtgcaca gcacgtcatc
     2401 cgcctccagg agccccgaga ttttcgtctc tccgacggcc agcgcgccca ggatgagcgc
     2461 gcggtgcgaa atcgacttgt cgcccggcac cagcgccacg cccttgagcg gccccgatcg
     2521 gcgcgcttcg agcggtcgcg agatttccgg cgccttcatg ggacgttcct acatggcttt
     2581 gacaggctgg cccaaccgtg gcaaacgacc cgaccttcga aaccggaaat cacaacggga
     2641 agcatgaccc tggtcaaggc ggatctcgga atcaaacgca tttgccccag ttgcaacgcg
     2701 cgcttctacg acctccagaa gcgcccgatc gaatgcccca aatgcgcgtt cacttttgag
     2761 cccgaggcgc tct
//
LOCUS       DASZMC010000054         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2782688,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000054 DASZMC010000000
VERSION     DASZMC010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2782688"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            475..804
                     /locus_tag="VG274_01025"
     CDS             475..804
                     /locus_tag="VG274_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05256.1"
                     /translation="MLHALQAEAVARAVATARAARDASLEAAEAQQVLHHAQTAGHFW
                     IDPLRERAERLAGRTVVLLVEAHARSVEAEGAARAVGLALHGKVWTPRDGAAEMEELP
                     GLRRAAG"
     gene            complement(1050..>1678)
                     /locus_tag="VG274_01030"
     CDS             complement(1050..>1678)
                     /locus_tag="VG274_01030"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWE05257.1"
                     /translation="FTIMLKGRPAQSASGNLPQRRCSMEMAYRERWQAEIVELQRILS
                     GFELKEECKWGKPCYTMDGKNVVIIQGFKEYCALGFFQGALLKDPKKLLVRLGQVQAA
                     RVIKFTSAKEVATLAATIKAYVREAVAAEKAGMKVETRPPELPVPGELKEKFRNDPRF
                     KRAFDALTPGRQRGYLFHFAGAKQPGTRTARIEKASPAIFEGRGLLER"
BASE COUNT          284 a          563 c          527 g          304 t
ORIGIN      
        1 ccgccatggt caatccgaga ttggcgatct cggcgaggtc gtcaggcttg ctgatctcca
       61 tgatgtccac gcaaggaccc tcgcccccgg ctgccgccaa cctcaatatc cactccacgc
      121 cccacccccg gtccgagaac aagagcaaaa ctactcggta cgctctggca cccccagttg
      181 cgcggcggtc ccgccgtcgt gcgcgaatcg gcgtaatctg gcccgacgtt gacagaggaa
      241 atccgccatg cgggtcgagc tggtactagg catgagcaac gtggtccggt ttccggtgga
      301 gcggcgcgcg cgtcccacgt tggggttgct gcgcgacatc gcaccggacg tgcgtgaggt
      361 gctcaacctg gtcgaggcat tcgggatgga agcgccgacg cgcgagcgcg cgatgccgcc
      421 acggcggaat acatcggtga tcaggtaccg gcgcatgggc aggagcgcga cggcatgctg
      481 cacgcgctgc aggccgaggc ggtggcgcgc gcggtcgcca cggcgcgggc ggcgcgtgat
      541 gcttcgctcg aggcggccga ggcgcagcag gtgctgcacc atgcgcagac ggcgggtcat
      601 ttctggatcg acccgcttcg cgagcgtgct gaacggctgg ccgggcgtac ggtggtgctc
      661 ctggtcgaag cgcacgcgcg gtcggtggag gccgagggcg cggcgcgggc ggtcgggctt
      721 gcccttcacg gcaaggtgtg gacgccacgc gacggggcgg ccgagatgga ggagctaccg
      781 gggctgcgcc gcgcggccgg ctgaactcgc cggccgcgcc ggctaacacc tcggcacaag
      841 gttatgacag gttgccggag cgttcttcct gcccgcccgg aaccacaacc gcccggtttt
      901 ggtgaggagg atcggctcgc tgctgaagga ccgctatggg tgatggaaag cggactctac
      961 tcacgcgcct caaacccgct gatccccggt gagtcgccaa gtcagcgcgg cgcatatctc
     1021 tcgctaaaca tccacagttt cacgatctat caccgctcca gaagccctcg tccttcgaag
     1081 atcgcaggcg acgccttctc gatccgcgcg gtccgcgtcc caggctgctt cgcgccggcg
     1141 aagtgaaaca ggtatcccct ttgccgaccc ggcgtcagcg catcgaacgc gcgcttgaac
     1201 cgcggatcgt tgcggaactt ctcctttaac tcaccgggca cgggaagctc tgggggcctc
     1261 gtctcgacct tcattccggc cttctcggcc gccacggcct cgcgcacgta ggccttgatg
     1321 gtggcggcca gcgtcgcaac ctccttggcg ctggtaaatt ttatcacccg cgctgcctga
     1381 acctgcccga gccgcaccag cagcttcttc gggtccttca gcagcgcacc ctgaaagaag
     1441 cccaacgcgc aatattcttt gaagccctga atgatgacga cgttttttcc atccattgtg
     1501 tagcagggct ttccccactt gcactcctct tttaactcaa acccagacaa gatcctttgc
     1561 agctcgacaa tctcagcctg ccaccgctct cgatacgcca tctccatgct gcatctccgt
     1621 tgtggcaagt tgcctgaggc tgactgcgct gggcgcccct tcaacatgat ggtgaaca
//
LOCUS       DASZMC010000055         5141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_21705,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000055 DASZMC010000000
VERSION     DASZMC010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21705"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..640)
                     /locus_tag="VG274_01035"
     CDS             complement(2..640)
                     /locus_tag="VG274_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWE05258.1"
                     /translation="MSEALTQPRMAPARQKLLDAAFAVIREKGYVATTVDELCARAGV
                     AKGSFFHHFDSKEALAVAAANYWSETTGVLFAEAPYHAHKDPLDRVLGYIDFRRALLT
                     GDVPDFTCLVGTMVQEIYETSPAIRDACDDSISSHAEKIETDISEAMTQRGIAATWTA
                     KSLALHTQAVLQGAFILAKAKGGADIAISSVDHLRRYIELLFQAPTKGKKAS"
     gene            729..1559
                     /locus_tag="VG274_01040"
     CDS             729..1559
                     /locus_tag="VG274_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HWE05259.1"
                     /translation="MSQHATAPKTSDIALEVAAAAPARFSARAVLIGDRLVVDRSKYS
                     DVVSAAPFAYRKGNGYVVVFRYGVVVLIGLSMEEEQDAIAQISAEMPKPAAEEERVAL
                     EIHADTEEGANANGVFFIKELTVAHALVLGDVLAKSVALARYEREIASVFDTIEPAAT
                     TLSLSGKLPRSRKSLLQLIGAALLAQHRVSGRIAFAEKPDMLWEHPELGRFFSRLEDE
                     YEIIERGTLLNGKVTVVGSAAETFTDLIDTARSARLELLIVVLILAELVIGAVTLFRP
                     "
     gene            complement(1564..2118)
                     /locus_tag="VG274_01045"
     CDS             complement(1564..2118)
                     /locus_tag="VG274_01045"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWE05260.1"
                     /translation="MSQHYHIFETARGFCAVAWSDAGVTRFRLPESSAALAERNTQRR
                     LPDASPAEPAPEIAKAIAAAKRYFEGERIDFADVALDLDGQEDFFRRIYTALRRVGWG
                     QTTTYGTLAKELGAGPEAARDVGQAMAKNPVPLIIPCHRVLAAGGKVGGFSAPGGAAT
                     KVHMLELEGVHVGPPEPAQQSLGL"
     gene            complement(2140..3411)
                     /locus_tag="VG274_01050"
     CDS             complement(2140..3411)
                     /locus_tag="VG274_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05261.1"
                     /translation="MSPRILRRWSFATLACIGSLTPAAATTPTLRGGVYVIVDATAQD
                     QYAVVTQALQTPGVDGLLIHLRWGQISTKLKTYDWTNLDQAIQLAMTANKRFEIGIVT
                     GGAMPQWISDPPPMGLGARHATFEVDAAGTCATFDTAPPYDPAYLAAFRDMLAQLAQH
                     LRSTHTYDHLAMLKLFGITTTTDELRLPATTAKCGDNVATWQSLSYRPAKMERGWKAM
                     LKAYLHHFPEKSFNIGFIGINAFPGIRDDGSAAATSQEAENLSEALAAKLIADAGAAR
                     PGHVALGFDSLTLNLPPDDKSYPKSRREFFSDAADANARLGWQTNELLGDYPGGGASC
                     GGSDPSNAVACTDWQEFRQMLFRGLYPHGKTNMPPSMQGVYLEMFPQNVAAFPQAVPP
                     AHKNLGVWNGEQSRAPQEAGGNRASSTPFSR"
     gene            complement(3508..4119)
                     /locus_tag="VG274_01055"
     CDS             complement(3508..4119)
                     /locus_tag="VG274_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE05262.1"
                     /translation="MQLYYSDLLSARKACAVARYLKAPVAFVYLDPAKGDHKTPAYVA
                     LNPNAKVPTLVKGDRVTWEADAILCQLSDDMGADLWPHDARQIDIVRWFSWNAQHLTR
                     AGGGLYFENIIKKRFNIGAPDPKVIEEEQNEFRRYAAVLSDHLKGRTWLVGDTLTVAD
                     FSVAVTLPYAQDAQIPLSEFPEICRWHDQLNELEAWREPFPSR"
     gene            4238..4948
                     /locus_tag="VG274_01060"
     CDS             4238..4948
                     /locus_tag="VG274_01060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE05263.1"
                     /translation="MSSHLLDTPIRAALTTRQAPLAEINGPARRYPTAIAPFAALEDE
                     TSESWAALHALMGPNEPAVFFTPEPVFAPPQFETVMAVTGEQMIGTPAEFSGPMPDMV
                     TLGAADVPEMIALVELTKPGPFGVRTHELGHFLGIRIGGRLAAMTGERMKPGSYTEMT
                     AVCVHPDFRGRGYAQALLAAVSRGIVARGEIPFLHVFSENHSAIALYKRQGMTIRRRI
                     HVTVLGRAGDNLKEKMPH"
     gene            complement(4945..>5141)
                     /locus_tag="VG274_01065"
     CDS             complement(4945..>5141)
                     /locus_tag="VG274_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HWE05264.1"
                     /translation="WFGHKPERFAEFKQRYKKELPPEALAELRKLGRGKTVTLLYGAR
                     DPEMNQAAVLLTLLRGTHKA"
BASE COUNT          939 a         1579 c         1611 g         1012 t
ORIGIN      
        1 ttcatgacgc tttctttcct tttgttgggg cttgaaacag cagttcgatg tagcggcgca
       61 ggtggtcgac acttgagatc gcgatatccg cgccgccttt tgccttggcg aggataaacg
      121 caccctgcag caccgcttgc gtatgcaggg cgagactttt ggcggtccat gtcgcggcga
      181 tgccgcgttg tgtcatcgcc tcagaaatgt ctgtttcgat tttttccgcg tgagagctga
      241 tgctgtcatc gcaggcatca cgaatggcgg gactggtttc ataaatctcc tgcaccatcg
      301 tgccgacgag gcaggtgaag tccggcacgt cgccggtgag gagcgcgcgg cggaaatcga
      361 tgtagcccag aacgcgatca agcggatcct tgtgtgcgtg atagggcgcc tccgcgaaca
      421 ataccccggt cgtctccgac cagtagttcg ccgctgcgac ggcaagggct tccttgctgt
      481 cgaaatgatg gaagaacgaa cctttggcaa cgcccgcccg cgcgcacaac tcatcgactg
      541 tcgtggcgac gtaacctttc tcgcggataa cagcgaacgc cgcatccagc agcttctgcc
      601 gggcgggagc cattcgcggt tgggtcaacg cctcactcat aaagtatgtg taccgactag
      661 tcggtacaat agtcaagcgc ctttttcagt cgcaatttga taggttaccg cgagcaggat
      721 ttgaccgtat gtctcagcac gccacagccc caaaaacttc ggacatcgcg ctcgaagtcg
      781 cagcagcagc gccagcacga ttctccgcgc gtgcggtgct catcggcgac cgtctcgtcg
      841 tcgatcggtc caaatacagt gacgtcgtat ccgcggcgcc gttcgcctat cgcaagggca
      901 acggttacgt cgtcgtgttc cgctatggcg tcgtcgtcct catcggattg tcgatggaag
      961 aagagcagga cgccatcgca caaatctctg cggagatgcc aaagcccgcg gcggaagaag
     1021 agcgcgttgc tctcgaaatc cacgccgaca cggaagaagg cgcgaacgcc aacggcgttt
     1081 tcttcattaa ggaactgacc gtcgcgcacg cgctggtgct cggcgatgtc ctggccaaga
     1141 gcgtcgcgct ggcgcgctat gagcgcgaga tcgcgtcggt gttcgatacg atcgagccgg
     1201 cagcgacaac gttgtcattg agcggcaagc ttccgcgctc gcgcaaatcg ttgctgcaac
     1261 tgataggcgc ggcgctcctc gcgcaacatc gcgtatcagg ccgcatcgcg ttcgcggaaa
     1321 agcccgacat gctgtgggag catccggaac tcggccggtt cttctcgcgc ctcgaagacg
     1381 aatatgaaat catcgagcgc ggcaccttgc tcaatggcaa ggtgacggtg gtcggaagtg
     1441 ccgcggaaac cttcacagac ctgatcgata ccgcccgctc cgcccggctc gaactcctga
     1501 tcgtcgtgct catcctcgcc gaactggtca tcggcgcggt gacgttgttc cggccctgaa
     1561 gcgtcagagt cccagtgatt gttgcgcggg ctccggcggg ccgacgtgga cgccttcgag
     1621 ttcgagcatg tgaacttttg tcgccgcgcc gccgggtgcc gaaaagccac cgaccttgcc
     1681 gccagccgcc agcacgcggt ggcaaggaat tatcagcggc accggattct ttgccatcgc
     1741 ctggccgaca tcgcgcgcgg cttcaggacc ggcgccgagt tctttcgcga gcgttccgta
     1801 agttgtcgtc tgtccccaac cgacgcgacg caacgctgtg tagatgcgcc ggaagaaatc
     1861 ctcctgccca tcgaggtcga gcgccacatc cgcgaagtcg atccgctcgc cctcgaaata
     1921 ccgcttcgcg gcggcgatgg cttttgcgat ctccggcgcc ggctcggctg gagacgcatc
     1981 gggcaggcgc cgctgcgtgt tgcgctcggc tagcgcagcg ctcgattccg gcaaccggaa
     2041 tcgcgtcacg cccgcatcgc tccacgcgac ggcgcagaac ccgcgcgcgg tttcgaaaat
     2101 atgatagtgc tggctcacgg catgactccc acgttcactc tatctcgaaa atggggtcga
     2161 ggacgcgcga tttccacccg cttcttgcgg cgcgcgggat tgctccccgt tccagacgcc
     2221 gagatttttg tgtgccggag ggaccgcttg cgggaatgct gcgacgttct gcgggaacat
     2281 ctccagatac acaccctgca tcgatggcgg catgttcgtc ttgccgtgcg gataaagacc
     2341 gcggaacagc atctgccgga attcctgcca atccgtgcag gcgaccgcgt tgctcgggtc
     2401 cgaaccgcca cacgacgcgc caccaccggg atagtcgccg agcaactcgt tcgtctgcca
     2461 gccgaggcgt gcattcgcat ccgccgcatc actgaagaac tcgcgccggc tcttcggata
     2521 ggacttgtcg tctggcggca aattcaaggt gagggagtcg aaaccgagtg cgacatggcc
     2581 cggcctcgcg gcgccggcat cggcgatcag ctttgcggcc agggcttccg atagattctc
     2641 ggcctcctgc gacgtcgccg cggcgctgcc atcgtcccgg atgccaggga aagcgttgat
     2701 gccgatgaag ccgatgttga acgatttctc cgggaaatga tggagatatg ctttgagcat
     2761 tgccttccac ccgcgctcca tcttcgccgg cctgtagctg agcgactgcc acgtcgcgac
     2821 attatcgccg catttcgcgg tcgtcgcggg caggcgcaat tcatccgtgg tcgtggtgat
     2881 gccgaacaac ttgagcattg cgaggtgatc gtatgtgtgc gtgctgcgca ggtgttgcgc
     2941 gagttgtgcg agcatatcgc ggaacgccgc aaggtaggcc ggatcgtagg gcggcgcggt
     3001 gtcgaacgtc gcgcaggtcc ccgcggcatc gacctcgaac gtggcatgcc gtgcgccgag
     3061 ccccatcggc ggcggatcgc tgatccattg gggcatggca ccgcccgtga cgatgccgat
     3121 ctcgaaacgc ttattggccg tcatcgcgag ctggatggcc tgatcgagat tggtccagtc
     3181 ataggtcttg agcttcgtgc tgatctggcc ccagcgcaga tggatcaaca gcccatcgac
     3241 accgggagtc tgcaaagcct gtgtgacgac ggcatattga tcctgtgccg tggcatcgac
     3301 gatcacatag actccgcccc gcagtgtcgg cgttgtcgcc gcggctggcg tgagcgatcc
     3361 gatgcacgcg agggtggcga acgaccagcg gcgtagaatc cggggtgaca tcggagagga
     3421 cctcttggcg cgacggaagt gtcgcccgga agtccgaaag gtttggttag caaagccgcg
     3481 cgctgtttaa ggaatgccgc atcgaaatca ccgcgacggg aacggttcgc gccaggcttc
     3541 gagttcgttg agttgatcgt gccagcggca aatctccggg aattcgctta gggggatttg
     3601 ggcgtcctgc gcatagggaa gcgtcacggc cacggagaaa tcggcaacgg tgagcgtatc
     3661 gccgacaagc catgtgcggc ccttgagatg atcgctcagc actgcggcat agcgtcggaa
     3721 ttcgttctgc tcttcctcga taactttcgg gtcgggcgcg ccaatgttga agcgcttctt
     3781 gatgatgttc tcgaaataga ggccgccgcc cgcgcgtgtg agatgctgcg cgttccagct
     3841 gaaccagcgc acgatatcga tctgccgcgc atcgtgcggc cacagatcgg cgcccatgtc
     3901 gtcggacagc tgacacagga tggcgtcggc ttcccaggtg acgcgatcac ctttcaccag
     3961 cgtcggcacc ttggcgttgg gattgagtgc gacataggcc ggtgttttgt gatcgccttt
     4021 cgcaggatcg agatagacga acgcgaccgg ggccttcaga tagcgcgcga cggcgcaggc
     4081 tttgcgggcg gagagcaggt ccgaatagta aagctgcatg gtcgttcccc gaatagctcg
     4141 tatgtccgtt cgtcgatgga cttcgcgatg cgcgacacta tgttcgcggc gactgtccga
     4201 aagaatttcc gccaacgcaa ccggcaggcg cgcgttcatg agttcacatc ttcttgacac
     4261 tccgatccgc gcggcattga cgacgcggca agcgccgctc gccgagatca acggtcccgc
     4321 gcggcgctat cctaccgcga tcgcgccgtt tgcggcgctg gaggatgaga cgtccgaaag
     4381 ctgggccgcg ctgcacgcct tgatggggcc gaatgagccc gcggtgttct tcacgccaga
     4441 gccggtcttc gcgccgccgc aattcgaaac cgtcatggcc gtgacaggcg agcagatgat
     4501 cggcacgccg gcggaattct ccgggccgat gccggacatg gtgacgctcg gtgccgccga
     4561 cgtcccggag atgatcgcgc tggtcgagtt gacgaagccc ggaccgttcg gcgtgcgcac
     4621 gcatgaactc ggacatttcc tcggcatcag gatcggcggg cgtctcgcgg cgatgacggg
     4681 tgagcgcatg aagccgggca gctacaccga gatgacggcg gtctgcgtgc atcccgattt
     4741 tcgcggccgc ggctacgcac aggcactgct cgccgccgtg tcacgcggga ttgtcgcgcg
     4801 cggcgaaatt ccattcctgc acgtattctc ggagaaccat tccgccatcg cgctctacaa
     4861 acgccagggc atgacgatcc gccgccgcat ccatgtgacc gtgctcggcc gggcaggcga
     4921 caatctcaaa gagaagatgc cgcattaggc tttgtgtgtt ccacgaagaa gcgtgagcag
     4981 caccgccgct tgattcattt ccggatcgcg cgcgccgtag agcagcgtca ccgtcttgcc
     5041 tctgcccagc ttgcgcaatt cggcgagcgc ttccggcggc aattctttct tgtagcgctg
     5101 cttgaactcc gcgaagcgct ccggcttgtg cccgaaccag c
//
LOCUS       DASZMC010000056         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3872593,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000056 DASZMC010000000
VERSION     DASZMC010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3872593"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VG274_01070"
     CDS             <1..1096
                     /locus_tag="VG274_01070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWE05265.1"
                     /translation="EGVDVVDFSAGEPDFPTPDNIKQAAIRAIEQNFTKYTPAGGTAE
                     CKQAVIERHKTEFGTDYKPSECIITVGGKHVLFNYTQALVNAGDEVIIPVPYWVTYKD
                     IVNFAGGKCVFVETDEKEDFRLTAAMIERNITPKTRIIIINSPSNPSGAVLADEEFAK
                     LYHITSKRGIFLLTDECYSHFLYDGSPYSVASIAGSKSTVLVAGTVSKTYAMTGWRIG
                     YGLAPEPIVTAMNKLQSHSTSNPTSISQKAAIEALRGAQDSVPQMLAEYRRRRDFVVP
                     RLRQIPGFECSMPAGAFYAYPSVRGALDRAGIKMPLEFADRLLKEANVAVVPGEAFGT
                     NDHVRISYATSMGELERGLDRIQKFVEQLG"
     gene            1093..1911
                     /locus_tag="VG274_01075"
     CDS             1093..1911
                     /locus_tag="VG274_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HWE05266.1"
                     /translation="MSQVLRLPRRAFPKVWGAPQTEPWFTNPNHEKIGEVWFESPPAT
                     PLLVKFLFTSESLSIQVHPDDAYARAHHNSFGKTEMWHILHADPGAKIAVGPRREITA
                     QDLAQAAGKKGILDLLQWVEVHPGDTWFIPAGTIHALGGGLALCEIQQLSDVTYRLYD
                     YGRDRELHIEHSLNVSRLTPCEGKRDPVALEPGRELLAECEYFRAERLTVSGAALCEP
                     RHICVVLEGEGRIGQETFGPGDAFAIGEEAMRIEGSRAIFVSVTSQCEPPVTRK"
     gene            1977..2762
                     /locus_tag="VG274_01080"
     CDS             1977..2762
                     /locus_tag="VG274_01080"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWE05267.1"
                     /translation="MKTLHNPAPLGPVSLASRIQNIQDTLLPLACCADGVECAGECER
                     SGNLGGDFFDFLLPRSKEVVAAIGNVAVAGVAGSILTTGLHVCLRLLGARGVTPADLS
                     SEINRTLWQMSPDHTYATMFCARINPRRQALTYVNAGHETALLMRGDSRMERLEPNGP
                     VLGFSRPFAYRERNTAFRPGDTLIAVSDGVAESPEGEPREQDLLRILKKRGAARVQEL
                     PGRILEAIRDLAGRSSKDRTVIVVRYRPYETRGAHAAAAATAA"
     gene            2849..>3219
                     /locus_tag="VG274_01085"
     CDS             2849..>3219
                     /locus_tag="VG274_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWE05268.1"
                     /translation="MPEWARRGPTHFESLNQLKSGLRALNLHTVCESARCPNIHECFH
                     RGAATFMILGNMCTRGCGFCSVPKGNPRIHDMRLDPNEPENVARMAADMNLRYVVITS
                     VNRDDLPDGGSAHFAETVREVR"
BASE COUNT          634 a         1093 c          897 g          595 t
ORIGIN      
        1 cgaaggagtc gacgtggtcg acttcagcgc gggcgagccc gacttcccca ccccggacaa
       61 catcaaacag gccgccatcc gcgccatcga gcagaacttc accaaataca cgccggccgg
      121 cggaaccgcc gagtgcaaac aggccgtgat cgagcgccac aagacggagt tcggcacgga
      181 ctacaagcct tccgaatgca tcatcaccgt cggcggcaag cacgtgctgt tcaattacac
      241 ccaggcgctc gtcaatgccg gcgacgaagt catcatcccg gttccctact gggtcactta
      301 caaagacatc gtcaatttcg cgggcggcaa gtgcgtcttc gtcgagaccg acgagaagga
      361 agatttccgg ctcacggcgg ccatgatcga gcgcaacatc acgcccaaaa cgcgcatcat
      421 catcatcaat tcgccgagca atccttcggg ggccgttctc gcggacgaag aattcgcgaa
      481 gctctatcac atcacctcaa aacgcggcat cttcctgctc accgacgagt gctattcgca
      541 cttcctatac gacggctcgc cttattccgt cgcctccatc gcaggctcga aatcgaccgt
      601 gcttgtcgcc ggaaccgttt cgaaaacgta tgccatgacc ggatggcgaa ttggctatgg
      661 tcttgcgccc gagccgatcg taaccgccat gaacaagctg cagagccatt cgacttcgaa
      721 ccccacttcg atctctcaga aagccgccat cgaagccctg cgcggcgccc aggattccgt
      781 gccgcagatg ctcgccgaat accgccgtcg gcgcgatttc gtggtgccac gcctgcgcca
      841 gattcccgga ttcgaatgtt ccatgcccgc cggagcgttc tacgcttacc cgagcgtgcg
      901 cggcgctctc gatcgtgcgg gaatcaagat gccgctcgaa ttcgccgatc gcctgctgaa
      961 agaggcgaac gtcgccgtgg ttcccggcga ggccttcggc accaacgatc acgtccgtat
     1021 ctcctatgcg acctccatgg gagaactcga gcgcggcctc gatcgcatcc agaagttcgt
     1081 ggagcagttg gggtgagcca ggtcttgcgg ttgcctcgca gagccttccc gaaggtctgg
     1141 ggcgcgccgc aaaccgagcc ctggtttaca aacccgaatc acgaaaaaat cggcgaggtg
     1201 tggtttgagt cgcctccggc cacgcctctt ctggtcaaat ttctcttcac ctccgaaagc
     1261 ctctcgattc aggtgcaccc ggacgacgca tacgcgcgcg cgcatcacaa ttcattcggc
     1321 aaaaccgaaa tgtggcatat tctgcacgcg gatcccggcg cgaaaatcgc cgtcggtccc
     1381 cggcgcgaga tcacagccca ggacctcgcg caggctgccg ggaaaaaagg gattctcgac
     1441 cttttgcaat gggtcgaagt acatccgggc gacacctggt ttatccccgc gggcacgatt
     1501 cacgctctgg gcggcggcct tgcgctctgt gagatccagc agctttccga tgtcacgtat
     1561 cgcctgtacg attacggccg cgaccgcgag ctgcacatcg agcacagcct gaacgtatcc
     1621 cgcctcactc cctgcgaagg caagcgcgat cccgtcgcgc tggaacccgg gcgagagctg
     1681 ctggccgagt gcgaatactt ccgcgcggaa cggctcacgg tatcaggcgc ggcgctctgc
     1741 gaaccccggc acatctgcgt cgtgctcgaa ggcgaaggcc gcatcggcca ggaaaccttt
     1801 ggcccgggcg atgctttcgc catcggcgag gaagcgatgc gaatcgaagg cagccgcgcg
     1861 atattcgtct ccgtaacgtc acagtgtgaa cctccagtaa cacgcaagta gctgcttttc
     1921 aattatttac cgaaagatct tccgtcgctt cacgctcctc gcgcgtcaga ccagatatga
     1981 agacgctcca caatcctgct cccttagggc ctgtttccct ggcgtcccgt atccaaaaca
     2041 ttcaggacac gctcctgccc ctcgcctgct gcgccgatgg cgtggaatgc gccggagagt
     2101 gcgaacggtc cggcaatctc ggcggcgatt ttttcgattt cctcctgccg cgtagtaagg
     2161 aggttgtcgc cgccatcggc aacgtcgccg tggcgggcgt cgcggggtcc attcttacca
     2221 ccggcctgca cgtctgcctc cggctgctcg gcgcccgcgg cgtcacaccc gccgatctca
     2281 gcagtgaaat caatcgcacg ctctggcaaa tgtcgccgga ccacacttac gccaccatgt
     2341 tctgcgcgcg cattaatccg cgccgccagg ccttaaccta tgtaaacgca ggccacgaaa
     2401 cggctctgct gatgcgcggc gattctcgca tggaacgtct cgagcccaac ggccccgtac
     2461 tcggattcag caggcctttc gcttaccgcg agcgcaacac ggcgttccgc ccgggcgata
     2521 ctctcatcgc cgtcagtgac ggcgtcgccg aatctccaga aggcgaaccc cgcgaacagg
     2581 atcttctccg tatcctgaaa aaacgaggcg ccgcgcgcgt tcaggagttg cccggcagga
     2641 ttctcgaagc cattcgcgat ctcgcgggcc gatcttcaaa agaccggacc gtcatcgttg
     2701 tccgctaccg cccgtacgaa acgcggggcg ctcacgccgc agccgccgcc acggccgcgt
     2761 agattctcct gactggcata atcggaactt gagttccgcg ttcgtccaga tcgagtcccc
     2821 cgtctcctcg aaaacgccgc gtccgcgcct gccggagtgg gcgcggcgcg gcccgactca
     2881 cttcgaatcg ctcaaccaac tcaaatcggg ccttcgcgcc ctgaaccttc acaccgtctg
     2941 cgaatctgcg cgctgtccga acatccacga atgcttccat cgcggggcgg cgacgttcat
     3001 gatcctcggc aacatgtgca cgcgcggctg cggattctgt tcggtgccga agggcaaccc
     3061 gcgcatccac gatatgcggc tcgatcccaa tgagccggaa aacgtcgcgc gcatggccgc
     3121 cgatatgaac ctgcgctacg tggtgatcac cagcgtgaac cgcgacgatc tcccggatgg
     3181 cggctccgcg catttcgccg aaaccgtgcg cgaggttcg
//
LOCUS       DASZMC010000057         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1672506,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000057 DASZMC010000000
VERSION     DASZMC010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672506"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VG274_01090"
     CDS             complement(<1..293)
                     /locus_tag="VG274_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05269.1"
                     /translation="MAALLFLVFANCGISIIGRGWTVSRLVVPYQPDITNPAGAISVV
                     FTDSNSQFLLRDFSVIARGATAAGTGCGTAITMNFMTDGVPGVPAPRKQRGGIV"
     gene            623..1156
                     /locus_tag="VG274_01095"
     CDS             623..1156
                     /locus_tag="VG274_01095"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HWE05270.1"
                     /translation="MSLSRVVFGLLLGGTVFGQTISAALADQPEILGVFKDWTAYKTG
                     AGDARVCYALSKPIQSEPRKLKRDAPYFLINDWPGRNAKAEPEIVPGYVYKEGSEVTA
                     QVGSDKFQLFTKNEGGAGGAWVEAQADEARLIDAMKAASQATVTGTSKRGTMTHDTYS
                     LAGLSDALDKVHQSCGM"
     gene            1153..2289
                     /gene="rlmN"
                     /locus_tag="VG274_01100"
     CDS             1153..2289
                     /gene="rlmN"
                     /locus_tag="VG274_01100"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWE05271.1"
                     /translation="MNGELAPAPLIGLGRRQLQETLAAAGIASKEAPMRARQLQNWIH
                     VHGTRDFAHMTSLAKGFRSFMAERFTLGRPDVVTEQISEDGTRKWLLRAGPGIEFETV
                     FIPDAHRGTLCVSSQVGCTLNCRFCHTGTQKLVRNLTPAEIVGQVMVARDALGDWPST
                     ATPRQITNVVMMGMGEPLYNFDNVKDALAIVTDGDGLALSRRRVTLSTAGVVPMIPRA
                     GAEIGSALAISLHSARDEVRDVLVPLNRKYPLVELMKAARAYPGLSNSRRITFEYVML
                     KDMNDSLADARDLVRLVRGLPAKINLIPFNPWPGAPYECSPWERIEKFAEIVNRAGYA
                     SPVRTPRGRDIMAACGQLKSESVRERASARHAQMRSAELARMPG"
     gene            complement(2302..>2949)
                     /locus_tag="VG274_01105"
     CDS             complement(2302..>2949)
                     /locus_tag="VG274_01105"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWE05272.1"
                     /translation="HIGEVIAPQYASDYPGVLDRLDASAFREFGERYLDTTRFRRTPG
                     HPFFTDKMPYNFLHVGLIHLILPNARIVDARRHPLACCFSNFSMNFKFGALFGNRLSE
                     LGQAYRDYVELMAHFDAVLPGRIHRVFYENLVREPEAEIRRLLDHLELPFEEACLEFH
                     KNERAMDSASSEQVRKPISTDAVEQWRNYEPWLGPLKSALGPVLDAYPDVPSFAS"
BASE COUNT          570 a          879 c          957 g          543 t
ORIGIN      
        1 acgatgccgc cgcgctgctt cctcggcgcg ggaacgccgg gcacgccgtc ggtcatgaaa
       61 ttcatcgtga tcgccgtgcc gcagcccgtt cccgccgccg ttgctcccct ggcgatgacg
      121 gagaaatcgc gcagcaggaa ctgggaattg gaatcggtga aaaccacgct gatggcgccc
      181 gcggggtttg tgatgtctgg ctgataggga acgaccagac gcgaaaccgt ccacccgcga
      241 ccgatgatcg agatgccgca gttcgcaaaa acgagaaaca agagagctgc cacttctgtt
      301 ccggggcaga gtcattaaat tcgcgccgaa cacgacgttt cgttttgtac cgtttccgat
      361 cgggcttgct ccccggcgaa agcgaacgcc agcgcaaagc tttgtgtgag ggagggaggc
      421 agatcagttt ggggatcgtg cgccgtggaa ccgccaatga atcggcccgt cattccgagt
      481 ttttgcgcag aaggcggtca aaactgaatg tttgtgcaaa gctgcccctg ttgcagggcc
      541 tgccaggtca tagtaaggcc cgatttccct gcgagcagag gtgctgcggg cgttccggtc
      601 atcgccaccc gaggagcttt tcatgtcgct gtcgcgcgtt gtttttggtc tgctgttggg
      661 cggaactgtg ttcgggcaaa cgatttccgc cgccttggcc gaccagccgg agatattggg
      721 cgtgttcaag gactggacgg cctataaaac gggcgccggg gacgcacggg tctgctacgc
      781 tctgtcgaag cccatccagt ccgaaccccg gaagctcaag cgcgacgcgc cttatttcct
      841 gatcaacgac tggccgggcc gcaacgcgaa ggcggaaccc gaaatcgtgc ccggctatgt
      901 gtacaaggag ggcagcgaag tcaccgccca ggtcggctcc gacaaattcc agcttttcac
      961 caagaatgag ggaggggcag gtggcgcctg ggtggaggcg caggccgacg aagcgcgttt
     1021 gatcgatgca atgaaggcag cgtcgcaggc gaccgtgacg gggacttcga aacgcgggac
     1081 gatgacccac gacacctatt cgctcgccgg cctttccgac gctctcgaca aggttcatca
     1141 aagttgcgga atgtgaacgg cgaacttgcg cccgctccgc tgatcggcct gggccgccgg
     1201 cagctccagg agacattggc tgcggcgggt attgcatcca aagaggcgcc gatgcgtgcc
     1261 cgccagcttc aaaactggat tcacgtgcat ggaacacgtg acttcgccca catgaccagt
     1321 ctggcgaagg gattccgcag cttcatggct gagcggttca cgctcggccg gccggacgtc
     1381 gtaaccgaac agatttccga agacggtacg cgcaaatggc tgctgcgcgc cggccccggc
     1441 atcgagttcg agacggtgtt cattccggat gcccatcgcg gcacgctttg cgtttcttcg
     1501 caggttggct gcacgctcaa ttgccgcttc tgtcacaccg gcacgcagaa gctcgtgcgc
     1561 aatctgacgc ccgccgagat cgtggggcag gtgatggtcg cccgcgacgc gctgggcgac
     1621 tggccttcca cggcaacgcc tcggcagatc accaatgtgg tgatgatggg catgggagag
     1681 ccgctctaca atttcgacaa cgtgaaggac gcgctcgcga tcgtgacgga cggcgacgga
     1741 ttggcgctct cgaggcggcg cgtcacgctt tcgacggccg gtgtcgtgcc gatgatcccg
     1801 cgcgcgggcg ccgagatcgg ttcggcgctg gcgatttcgc tgcattcggc gcgcgatgag
     1861 gtgcgcgacg ttctggtgcc tctgaaccgc aaatatccgc tcgtggaact catgaaagcg
     1921 gcgcgcgcct atcccgggct ctccaattcg cggcgcatca cgttcgaata tgtgatgctc
     1981 aaggatatga acgacagcct tgccgatgcg cgcgacctcg tgcgcctggt ccgcggcctt
     2041 ccggcgaaga tcaacctcat tcctttcaat ccctggccgg gcgcacctta cgaatgctcg
     2101 ccctgggagc ggatcgagaa attcgccgag atcgtcaacc gtgcgggcta cgcctcgccg
     2161 gtgcgcaccc cgcgcggacg cgacatcatg gcagcctgcg gccagctgaa gagcgaaagc
     2221 gtgcgcgaac gcgcgagcgc gcgtcatgcg cagatgcgct ccgcggaact ggcgcggatg
     2281 ccaggctgag cgcgaccgaa ctcaagatgc gaaggaaggt acgtcgggat aggcgtccag
     2341 aaccggtccc agcgctgact tgaggggtcc gagccatggt tcatagttac gccactgctc
     2401 gacggcgtcg gtggagatcg gcttgcgtac ctgttccgaa cttgccgagt ccatggcgcg
     2461 ctcgttcttg tggaattcca ggcacgcctc ctcgaagggc agctcgagat gatcgagcag
     2521 ccggcggatt tccgcctccg gctcacggac caggttttca tagaagaccc ggtggatgcg
     2581 tcccggcagc actgcgtcga aatgcgccat cagctccacg taatcgcgat acgcctggcc
     2641 gagttcgctc agccggtttc cgaaaagtgc cccgaattta aagttcatcg agaaattgga
     2701 aaagcagcag gcgaggggat gccggcgcgc gtcgacgatc cgggcattgg gcaggatcag
     2761 atgaatgagc ccgacatgga gaaaattgta aggcatcttg tcggtgaaga acgggtgtcc
     2821 gggggtgcgc cggaaccgcg tcgtgtcgag atagcgttcg ccgaattccc ggaacgcgga
     2881 cgcatcgagc cgatccagta cgccgggata gtccgaggca tattgcggcg cgatcacttc
     2941 gccgatgtg
//
LOCUS       DASZMC010000058         6177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1672547,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000058 DASZMC010000000
VERSION     DASZMC010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672547"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..180)
                     /locus_tag="VG274_01110"
     CDS             complement(7..180)
                     /locus_tag="VG274_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05273.1"
                     /translation="MPKNVAQWTGIAVTVVIVLGRDLDVLTALPLGVMAGVLATSLVA
                     LADQRMPARIKRK"
     gene            complement(265..1755)
                     /locus_tag="VG274_01115"
     CDS             complement(265..1755)
                     /locus_tag="VG274_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWE05274.1"
                     /translation="MNAREILSKVGVEAISGDHAVRSPIDGEEIGRVAFDNDASIQSK
                     IAASVDAFRVWRDVPAPRRGELVRLFGEELRAHKELLGRLVTLENGKILQEGLGEVQE
                     MIDICDFAVGLSRQLYGLAIASERPGHRMMEQWHPAGPVAVISAFNFPVAVWAWNAAL
                     ALVCGDPVIWKPSEKTPLTAIATQALFERAATRFGSAPDRLSQIVLGAREAGEALARD
                     HRIPVVSATGSTAMGRALAPVVASRFGRAILELGGNNAMIVCPSAKLDLALRAILFAA
                     VGTAGQRCTSLRRLFVHESIYDRLLEALKSSYARLAIGNPLDAGTLVGPLIDDRAFEQ
                     MEHALAVAKDEGGRISGGGRVLADRFPNARFARPAIVEMPAQTDIVRRETFAPILYVM
                     RYREFTDALSEHNAVPQGLSSCIFTQDMSQAERFVAAAGSDCGIANVNIGPSGAEIGG
                     AFGGEKETGGGRESGSDAWKAYMRRQTATINYSDALPLAQGIRFDL"
     gene            complement(1893..2972)
                     /locus_tag="VG274_01120"
     CDS             complement(1893..2972)
                     /locus_tag="VG274_01120"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWE05275.1"
                     /translation="MKNVALVGGGKIGIAITELLAGTGDYRVTVFDRDAESLERMPRR
                     NVETRRLEISDARDFSRALEGQDIVLSASPYSLTATIAEAAKRARVHYLDLTEDVEST
                     RTIKRLAEGAETAFIPQCGLAPGFISIAAFDLTRKFEKLRDVEMRVGALPVFPTNALK
                     YNLTWSTDGLINEYCNPCESIRDAHRGEVPALEELEAFSLDGIAYEAFNTSGGLGTLC
                     DTLEGKVENLNYKTVRYPGHRDIVKMLVRDLRLGMRRDVLKDVLETAIPITYQDVVLI
                     FVTVSGWMEGHLTQESYAKKIYSQTVGDRLMSAIQITTAAGICAMCDLLVEGKLPQKG
                     FVRQEEAKFPDFLGNRFGRYYARGM"
     gene            3140..3565
                     /locus_tag="VG274_01125"
     CDS             3140..3565
                     /locus_tag="VG274_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWE05276.1"
                     /translation="MRDTDSRLIALLRANAREPVASLARKLNLARSTVQERIARLERG
                     GVIKGYTLLLSEEAESKRLRAVVMMAADPKQADRVSAELKRMPEVRALSAVSGAHDKM
                     AIVETDTAARMDAALDRIGKATGVARTLSSIILSEKFSR"
     gene            4082..5584
                     /locus_tag="VG274_01130"
     CDS             4082..5584
                     /locus_tag="VG274_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HWE05277.1"
                     /translation="MQEPSHTQSDPRSGHVARALDRARRPNREHMQALKRRVPFWFVV
                     ALLSGFAALQIVLNPFGFSDLTQRYTQDISNLLISGPYLYPDSGRQQISVALVEDDTH
                     SNLQMPWPWPYGVQARALDAILALKPRAVVVDVMFIDPRKDDTLPELVDEIGRYKRAG
                     VPLYLTGATDAPPGSPPVRRELAATGVRILDPTLLVNQGIVRQYPASGQCFSAKVQDQ
                     SCLSLALQIYKDLFPQAPLAPIRGLMEIVWGTRTNPINSKWMRVTDDSGVSHACGENQ
                     DISWSRRIYLAFFDPGSVRSACPYTGVIPAQALLDGREDPDITALAHNRIVFYGASLS
                     GVQDKSFTPVNGLIASVYVHAMALDNLISFHGRPEQNVVSIGNITLDNNTAQMIAIVP
                     IILILAWMHMRRLRSRRVRRQRGAMLEYLLDKAFQSAWHWLAFALALGVGLVLTLAAG
                     LSVANWVEVVFVSVELAAMLLIGLPDAVWGYLHHVAGGIPQFPTAQEEGG"
     gene            5584..5979
                     /locus_tag="VG274_01135"
     CDS             5584..5979
                     /locus_tag="VG274_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05278.1"
                     /translation="MRYPSCLIFCGAILSATPALADDTCSTSTGDVVLAARTPQVDLY
                     DAAMGKRVMTMDADKFPGCAPILGRAANMMLEVSVGGTKYWVPPHMVKYRFAGSLPAI
                     CRNLAMGSNETKAGSTRGLGEGCPKPGGK"
     gene            5979..>6177
                     /locus_tag="VG274_01140"
     CDS             5979..>6177
                     /locus_tag="VG274_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05279.1"
                     /translation="MRRISLVALAAGAILALPFHAAASGYPGRGKSIQELSPASDYND
                     LLQTRTRALGIMRARAQGFVPS"
BASE COUNT         1100 a         2012 c         1928 g         1137 t
ORIGIN      
        1 cgacggtcat ttccgcttga ttcgggcggg catgcgctgg tcggcgagcg ccacgagcga
       61 agtcgcgagg acgccggcca taacgccgag ggggagcgcg gtgagcacat cgaggtcacg
      121 gccgagcacg atgacgaccg tcacagcgat cccggtccac tgcgccacgt tcttcggcat
      181 ggtctgcctg cgagatcgaa tgccctcccg aagcctaacg cggcacgggc gcaatcgaag
      241 cacaggctta ataaagggtt aatttcagag gtcgaagcgg attccctgtg ccagtggcaa
      301 cgcgtcggaa tagttgatgg tcgcggtctg ccggcgcata taggccttcc aagcgtcgga
      361 gcccgattcc cgcccgccgc ccgtctcctt ctcgccgcca aacgccccgc cgatctccgc
      421 acccgacggt ccgatattca cattggcgat cccgcaatcg gaacccgccg cagcaacgaa
      481 gcgctcggcc tgcgacatgt cctgcgtgaa gatgcacgac gacaatccct gcggcaccgc
      541 attgtgttcc gacaacgcgt cggtgaactc gcggtagcgc atgacgtaga gaatgggcgc
      601 gaaagtttcg cgccgcacga tgtccgtctg ggccggcatc tcgacgattg cgggcctcgc
      661 gaaacgcgcg ttcggaaatc tgtcggccag cacgcgtccg ccgccgctga tgcgtccgcc
      721 ctcatccttc gccactgcga gcgcgtgttc catctgctcg aatgcgcggt catcgatcag
      781 cgggccgacc agtgttccgg cgtcgagcgg attgccgatt gcgagccgcg cgtatgacga
      841 tttcagggct tccagcagcc tgtcgtaaat cgattcatgc acgaacaacc gtctcaggct
      901 cgtgcagcgc tggcctgcgg tccccactgc ggcgaagagg atcgcccgca gagcgaggtc
      961 aagcttcgcc gatggacaga cgatcatcgc gttgttgccg ccgagttcga ggatcgcgcg
     1021 gccgaaccgc gacgccacca cgggcgccag cgcccgcccc atcgcggtcg agccggtggc
     1081 gctgacgacc ggaatgcggt ggtcgcgcgc cagcgcctcg cccgcctcgc gggcgccaag
     1141 cacgatctgg ctgagccggt cgggcgccga accgaatcgc gtggccgcgc gctcgaacag
     1201 ggcctgcgtc gcgatggcgg taagcggagt cttttccgac ggtttccaga tgaccggatc
     1261 gccacagaca agcgcaagcg ctgcgttcca cgcccacacc gcaaccggaa agttgaacgc
     1321 ggaaatgacg gcgacaggcc cggccgggtg ccattgctcc atcatgcgat ggcccgggcg
     1381 ctccgaagca atcgcgagtc cgtagagctg gcgcgacaga ccgaccgcga aatcgcagat
     1441 gtcgatcatt tcctggactt cgccgagtcc ttcctggaga atcttgccgt tctcgagcgt
     1501 cacaagccgt ccgagcaact ccttgtgcgc gcgcaactct tccccgaaca gccgcacaag
     1561 ctcgccacgc cgcggcgccg gaacatcgcg ccaaacgcga aacgcgtcga cgctcgcagc
     1621 aatcttcgat tggatcgatg cgtcgttgtc gaaagcaacg cgtccaattt cctccccgtc
     1681 gatcggcgaa cggacggcgt gatcgcccga gatcgcctcg acgccaacct tcgaaagaat
     1741 ctcgcgcgcg ttcatttgtt cctctccttc attcctgcgt gagcacacgt cgtttcatgg
     1801 ttcagtaggt cgcgaatgcg ctctctactt tcctccccca acgttggagg aggaaaacga
     1861 ttgctcgcgt tcgccctcca gggcggcgga gcctacattc ccctcgcgta ataacgcccg
     1921 aagcggttgc cgaggaaatc cgggaacttc gcttcctcct ggcgcacgaa acccttctgg
     1981 ggcagcttgc cctcgacgag aaggtcgcac atcgcgcaga tgccggcggc ggtcgtgatc
     2041 tggatggcgc tcatcagccg gtcgcccacc gtctggctgt agattttctt tgcgtagctc
     2101 tcctgggtca ggtgaccttc catccagccg gagaccgtca cgaagatcag cacgacgtcc
     2161 tggtaggtga tcggaatcgc cgtctccagc acgtctttca gcacgtcgcg gcgcatgccg
     2221 agcctgaggt cgcgcaccag catcttcacg atgtcgcggt ggccgggata gcgaacggtc
     2281 ttgtagttga ggttctccac cttgccttcg agcgtatcgc acagcgtgcc cagcccgccc
     2341 gacgtgttga acgcctcgta ggcgatgccg tcgagcgaaa aagcttcgag ttcttccagg
     2401 gccggtactt cgccgcgatg ggcgtcgcgg atcgactcgc agggattgca atattcgttg
     2461 atcagcccgt cggtcgacca ggtcagattg tatttgagcg cgttggtcgg gaagacgggc
     2521 aatgcgccaa cccgcatttc gacgtcgcga agcttctcga atttcctggt caggtcgaaa
     2581 gcggcgatcg aaatgaaccc cggcgcgagt ccgcattgcg gaatgaaggc cgtttccgcg
     2641 ccttcggcca gccgcttgat cgtccgcgtg ctttcgacgt cttcggtgag gtcgagataa
     2701 tgcacgcggg cgcgcttggc cgcctcggcg atggtcgcgg tgagcgaata gggggacgcg
     2761 gaaagcacga tatcctggcc ctccagagcg cgcgagaaat cgcgcgcgtc ggagatctcg
     2821 agccgccgcg tctcgacatt gcgccgcggc atccgttcga gactctcggc gtcgcggtcg
     2881 aacaccgtga cgcggtagtc gccggtgccc gcgagcagtt ccgtgatggc aatgccgatc
     2941 ttgccgccgc cgacaagcgc aacgttcttc atgggaattc ctccggttct gaatgcatgc
     3001 ttcgacaggc tcagcatgag gagtactctg ggactcctcg ccctgagctt gtcgaagggt
     3061 gcggccggaa aggagacggc aaagcgccgg aaaatgggct agcatccggc gaatcgcagg
     3121 gaactccggc ggatcgccca tgcgcgacac cgacagcagg ctgattgccc tcttgagggc
     3181 caatgcgcgc gagcccgtgg cctcgctggc gcgcaagctc aaccttgcac gttcgacggt
     3241 gcaggagcgc atcgcgcgcc tcgagcgcgg gggcgtcatc aaaggctaca ccttgctcct
     3301 ctctgaggag gcggaatcga aacgcttgcg cgcggtggtg atgatggcgg ccgatcccaa
     3361 acaggccgac cgcgtcagcg cggaattgaa gcgcatgccg gaggtgcgcg cgctctccgc
     3421 cgtctccggc gcgcatgaca agatggcgat cgtggaaacc gatacggccg cccgcatgga
     3481 cgcggcgctc gaccgcatcg gcaaagccac cggcgtcgca cgcactttgt cctcgatcat
     3541 cctgtcggag aagttttcgc gctgaatcac agcatgatcg ggttggattg tgcgccgcga
     3601 agaatcgaaa gcccgggact taagcccggg ctttcctctc ccgtacgcac gggaattatg
     3661 gcgagcgatg ctcgcttgtc gtgatttcga ttttttgggt ggcaaccggc gttgtcggcg
     3721 ccggattgtg gctcagcttg cgcagagccc gggccttccg cccggcggag tgtcttccat
     3781 gcggaatcct cctgttcatt ggttacgact gccgaccaca aaggaaggcg cgcaaggttt
     3841 cgaaggggtc tgggcacttc cttgcagtgg cgacgatcga attttccgcc tccaaacggc
     3901 tcctgtcaac gccgaagagc cagggagtca gagaagcttt tgaacatttg aaccctcggg
     3961 cgaccccctt tcgcccaaag gcgggaccat gtgcgggtta tgcccgagcg aacccggatt
     4021 catcccgcca tggcgttccg aattgtgcta ggctgaaagc ctccaaaggg gcgggggcag
     4081 cgtgcaggag ccttcacaca cgcaatccga tccgcgcagc gggcacgtcg cacgggcgct
     4141 cgatcgtgcg cggcgaccga accgcgagca catgcaggcg ctcaaacggc gcgtgccgtt
     4201 ctggtttgtc gtcgcgctgc tgtccggatt cgcggcgctg cagatcgtgc tgaacccgtt
     4261 cggcttcagc gacctcaccc agcgctacac acaggacatt tccaacctgc tcatcagcgg
     4321 gccgtatctc tatcccgaca gcggacgcca gcagatctcg gtcgccctcg tggaggacga
     4381 cacccattcc aatctgcaga tgccctggcc gtggccctac ggcgtgcagg cgcgcgcgct
     4441 cgacgcgatt ctcgcgctca agccgcgcgc ggtcgtcgtc gatgtgatgt tcatcgatcc
     4501 gaggaaggac gacacgcttc ccgaactcgt cgacgaaatc gggcgctaca aacgcgccgg
     4561 cgttccgctc tatctcactg gcgcaaccga tgcaccgccc ggctcgcctc ccgtgcgcag
     4621 ggagctcgcg gcgaccggcg tgcgcattct cgatcccacc cttctcgtca atcagggcat
     4681 cgtgcggcaa tacccggcga gcgggcagtg cttcagcgcg aaggttcagg accaatcctg
     4741 tctctcgctg gcgctacaga tctacaagga cctctttccg caggcgccgc tcgcgccgat
     4801 ccgcggattg atggaaatcg tctggggcac acgcaccaat ccgatcaact ccaaatggat
     4861 gcgggtaacg gatgacagcg gcgtgtcgca cgcctgtggc gagaaccagg acatcagctg
     4921 gagccggcgc atctatctcg ccttcttcga tcccggcagc gtgcgctcgg cgtgccccta
     4981 cacgggcgtg atcccggccc aggcgctcct cgacggccgt gaagatccgg atatcacggc
     5041 gctcgcgcat aatcgcattg tcttctatgg cgcgtcgcta tcgggcgttc aggacaaatc
     5101 ctttacgccg gtcaacggcc tgatcgccag cgtgtacgtg cacgcgatgg cgctcgacaa
     5161 tctgatcagc ttccacgggc ggcccgaaca gaacgtcgtc tcgatcggga acatcacgct
     5221 cgacaacaac acggcgcaaa tgatcgcgat cgtgcccatc attctgattc tcgcctggat
     5281 gcacatgcgg cgtctgagga gcaggcgcgt tcggcgccag cgcggcgcca tgctcgaata
     5341 cctcctggac aaagcgttcc agtcggcctg gcactggctt gcctttgcac tggcgctggg
     5401 ggtcggactg gtgctgacgc tcgctgccgg cctttcggtc gccaactggg tcgaagtcgt
     5461 attcgtctcc gtcgagcttg cggcgatgct gctgatcggt ttgcccgatg cggtgtgggg
     5521 atatctccat cacgtcgcgg gcggcattcc acaatttccc actgcacagg aggagggggg
     5581 ctgatgcggt acccgtcatg cttgattttt tgcggcgcaa ttctgtcggc cacaccggcg
     5641 ttggccgacg atacgtgcag cacatccacg ggcgacgtgg ttctggctgc gcgcacgccg
     5701 caggtcgatc tctacgacgc ggcgatgggc aagcgcgtga tgacgatgga cgccgacaag
     5761 tttccgggct gcgcgccgat tctcggccgc gccgccaaca tgatgctcga ggtcagcgtt
     5821 ggcggcacga aatactgggt gccgccgcac atggtgaaat accgcttcgc gggatcgctg
     5881 cccgccatct gccgcaatct cgcgatgggc agcaacgaga ccaaggccgg atcgacgcgc
     5941 ggtctgggcg aagggtgtcc gaaaccagga ggcaaatgat gcgcaggatt tctcttgttg
     6001 cgctcgcggc gggcgcaatt ctcgcgctcc cgtttcacgc ggcggccagc ggatatcccg
     6061 gccggggcaa atcgatccag gaattgtcgc ccgcctccga ttacaacgac cttttgcaga
     6121 cgcgcacccg cgcgctcggc atcatgcgcg cccgcgcgca gggcttcgtg ccgtctc
//
LOCUS       DASZMC010000059         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_846332,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000059 DASZMC010000000
VERSION     DASZMC010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846332"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VG274_01145"
     CDS             <1..220
                     /locus_tag="VG274_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05280.1"
                     /translation="FSLGIGTTGSKVPCLSLIHARAASKPDAMPVPLQVWADTRPEAH
                     TRASVSTSSEWLRLFISGLLAFAFMDLI"
     gene            complement(772..>1821)
                     /locus_tag="VG274_01150"
     CDS             complement(772..>1821)
                     /locus_tag="VG274_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWE05281.1"
                     /translation="AWEFEFRRGDESPIVMLEQSPRCDAIIGNLDGIGPRETAALRRR
                     AAPTVLIEEYNDSGYPTVVGDNRAIGRLAFEHLRTKGFCRYAYFGWDGLRLFRERGDA
                     FGAAVREAGLEFHDSPRIDHELISPEHEPWVQAWLRNLPKPIGVLAGNADLARRIVAM
                     SRMMNLLVPEEVAVIGVDNETLDCELSWPPLSTIDHGMERAGYEAAALLHRMMTGARA
                     PKKPIVVQPVSVIERQSTDTLAVEDPDVRAAVQLIRQRATQGLSVKDMLDLLPVTRRK
                     LEIGFRRSLGRSIHSELTRVRLERAKLLLRTTEMAMPQIAAACGFEYASRLSILFVQT
                     IGMTPTAYRRQQRGE"
BASE COUNT          324 a          626 c          511 g          360 t
ORIGIN      
        1 cttctccctt ggcatcggga cgacaggttc aaaggttccg tgcctgagcc tgatccatgc
       61 tcgcgccgct tcaaagccgg atgccatgcc ggtcccctta caggtctggg ccgacactcg
      121 tcccgaagca cacaccagag cctcggtttc gacatcatct gaatggttac gactcttcat
      181 cagcggttta cttgcattcg ccttcatgga cctcatctga cgcggtcact gccacgcctt
      241 ttggctcgac gttcaccacg actggctctt aaccagcgca gctcgagttg atttgcggcc
      301 tgaccctgtc gcccgaccgc gagagacctc ctctcatctc aagcacagct ttcctcatca
      361 cacggtgtgc ccacaaatgc gggaccggcg cagatgtgat tagccggacg cgcaagcgac
      421 ggtgggccac tctcgcgaaa ccgcgtcgat tgccgcatca gcccaccgtc gctttcgcgt
      481 ccggctaatc atcacctgct attacctggc ctcccggaaa aatgggcaca ccctggaaaa
      541 ccgcgatgcc ggggaaattc aaagtcggct ataacccttg agcgttgagc tctggaaata
      601 agtacgggga tgcctcgaaa taggcgggac gtgaaacggt gtcggggtcc cttctttttc
      661 gcctcgacgc ctcgacgcgc ttttcagctt ctctaggccc ctcgctatgc caattccgct
      721 gatggctatt ggttctgacc gcgccgcgcg gctggtcgcg cagcggcggc gtcattcccc
      781 gcgctgctga cggcgatagg cggtgggggt catgccgatg gtttgcacga acaatatgct
      841 gaggcgcgag gcatattcga agccgcaggc cgcggcgatc tgcggcatcg ccatttccgt
      901 cgtgcgcaag agaagtttgg cccgttcgag gcggacgcgc gtcaattccg agtgaatgct
      961 ccggccgagg cttctgcgaa acccgatctc gagtttccgg cgcgtgaccg gcaacaagtc
     1021 caacatgtcc ttgacgctca gcccctgcgt ggcgcgctgg cggatgagtt gcacggccgc
     1081 gcgcacgtcg ggatcttcca cggccagcgt gtcggtcgat tgccgttcga tgacgctgac
     1141 cggctgcacc acgatcggct ttttcggcgc ccgtgcgccg gtcatcatcc gatgcagcag
     1201 cgccgccgcc tcgtaaccgg cgcgctccat gccatggtcg atggtcgaca gcggcggcca
     1261 ggacaattcg cagtccaggg tttcgttatc cactccgatc accgccactt cttcgggcac
     1321 gagcagattc atcatccggc tcatcgccac gatccgccgg gccaggtccg cattgcccgc
     1381 cagcacgccg atcggcttgg gcaaatttcg cagccacgcc tgcacccacg gctcgtgttc
     1441 cgggctgatc agctcgtgat cgattctcgg cgaatcatga aactccagcc ccgcctcgcg
     1501 aaccgccgcg ccgaaggcgt cgccccgctc gcgaaacagc cgaaggccat cccagccaaa
     1561 atatgcatat cggcaaaatc ccttagtgcg gaggtgctcg aacgccagcc ggccgatggc
     1621 ccggttgtcc cccaccaccg tgggatatcc gctatcgttg tattcctcga tcagaaccgt
     1681 cggcgccgcc cgccggcgca gcgcggccgt ctcgcgcggc ccgatcccgt cgagatttcc
     1741 gataatcgca tcgcaacgcg gcgactgctc caacatcacg atgggcgatt catccccccg
     1801 cctgaactca aattcccacg c
//
LOCUS       DASZMC010000060         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3330805,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000060 DASZMC010000000
VERSION     DASZMC010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3330805"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VG274_01155"
     CDS             complement(<1..181)
                     /locus_tag="VG274_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05282.1"
                     /translation="MDTRCFDVRVSDGIARIELKRGDELNTMVPEFWRELPDIVRSID
                     EDSKARVIVISSTGKH"
     gene            303..1163
                     /locus_tag="VG274_01160"
     CDS             303..1163
                     /locus_tag="VG274_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWE05283.1"
                     /translation="MEMAINYDEIMQLQSSGNPFSYTDRDTMLYALGVGFGRDPLNEA
                     ELPFVYENGLRTIPTLSTVVAWGSAIMGRTGINYLYVLHGEQRLIVHKPLPVAAQLLA
                     DERVTGAFDKGKDKGAFVIIEKTLRLKDTGEKLCTLSATTVARGDGGFGGPKEGAPEP
                     HALPTRAPDLVQDCDTRPDQAFLYALSGDRNPLHRDPRVAKMAGFPRPILHGLCTYGT
                     ACRAIVSTVCKYDANRIAGFDARFSAPVFPGETIATEIWVDGNVVSFRSKVKERDVVV
                     ISNGKCTLSS"
     gene            1120..>2121
                     /locus_tag="VG274_01165"
     CDS             1120..>2121
                     /locus_tag="VG274_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWE05284.1"
                     /translation="MLSSSATASARFLPDENPVAAAEALIAAGHASEAAALLGKYIAA
                     NRGGLLMRLTMQKALAASGDKAGALAMARETALANANIALAALALGDALLAADQLPTA
                     IGEFQRALRLDPQLERARAQLGAAWLAAGEAQKALEGWSVMENRSSLEALAPAIADAE
                     KSLERPRSDPRYVRHLFDQFSASYDLRMTTELRYCAPSILRQLAELLGLDRRGPQAIL
                     DLGCGTGLMGVAVRDWADRLDGVDLSPAMIEKARSRAIYDELSVGDIRNWLAAREYRY
                     DLVFAADTLVYLGDLGPLFEVVARCLAHDGQFLFTFEKSSGPGFELGPKRRWRHSETY
                     "
BASE COUNT          389 a          650 c          678 g          404 t
ORIGIN      
        1 agtgcttgcc cgtcgaggaa atgacgatga cgcgcgcctt cgagtcctcg tcgatggaac
       61 gcacgatgtc gggcagttcg cgccagaatt ccggcaccat ggtgttgagt tcgtcgccgc
      121 gtttcagttc gatccgggca attccgtccg agacgcgcac gtcaaagcag cgagtgtcca
      181 tgggcgtcgg cctttccgtc gatgcagatg cgatatagca caacggggcg caatgcgggc
      241 gccgcccttg cggcaaacgg tctccggcgc tctactcgcc cgcgacgcaa cagccggacg
      301 cgatggaaat ggcgatcaat tacgacgaaa tcatgcagct ccagtcgagc ggcaatccgt
      361 tcagctacac ggaccgcgat acgatgctct atgcgctggg cgtcggcttt gggcgcgatc
      421 cgctgaacga agcagagctg cctttcgtct atgaaaacgg cctgcgcacg attcccaccc
      481 tttctaccgt ggttgcatgg ggcagcgcca tcatgggacg cacgggaatc aactatctct
      541 atgtcctgca cggcgagcag cggctcatag tgcacaagcc ccttcctgtt gccgcgcagc
      601 tccttgccga cgagcgcgtg accggcgcct tcgacaaggg caaggacaag ggtgcattcg
      661 tcatcatcga gaagaccctt cgtctcaagg acacggggga gaaactctgc accttgagcg
      721 ccacgaccgt tgcgcggggc gatggcggct ttggcggacc gaaggaggga gcgcccgaac
      781 cgcacgcgtt gccaacgcgc gcacccgatc tcgtgcagga ttgcgacacg cggcccgacc
      841 aggcgtttct ttatgcactc tcgggcgacc gcaatccgct gcatcgcgat ccgcgcgtgg
      901 ccaagatggc gggatttccg cgccccattc tccacggact ctgcacctac ggtaccgcct
      961 gccgtgcaat cgtgtcgacg gtctgcaaat acgatgcgaa ccggatagcg ggtttcgacg
     1021 cacggttttc ggcgccggta tttcccggcg aaacgattgc aaccgaaatc tgggtggatg
     1081 gaaacgtggt gtcgttccgg tcaaaggtaa aggagcgcga tgttgtcgtc atcagcaacg
     1141 gcaagtgcac gctttcttcc tgacgaaaac ccggttgcgg cggcggaggc gttgatcgcc
     1201 gccggccacg cgagtgaagc cgcggcactt ctggggaagt acatcgcggc caaccgcggt
     1261 ggtctcctga tgcggctcac gatgcagaag gcattggcgg catcgggcga caaagccggc
     1321 gcgctcgcga tggctcgcga gacggcgctc gccaatgcca atattgcgct tgcggcgctc
     1381 gcgctgggcg acgcgctcct cgcggcggac cagctgccga ctgcgatcgg cgaatttcag
     1441 cgggctctgc ggctcgaccc gcagcttgaa cgcgcacgcg cgcagcttgg cgcggcgtgg
     1501 ctcgccgccg gcgaagcaca aaaagcgctc gaaggctgga gtgtgatgga aaacagatcg
     1561 tctctggagg cgctcgcacc ggcgattgca gatgccgaaa aatctcttga gcggccgcgc
     1621 agcgatccgc gttacgtgcg tcatctcttc gatcagtttt cggcaagcta cgatttgcgc
     1681 atgacgacgg agctccgata ttgcgcgccg tcgattcttc ggcagcttgc ggagctcctg
     1741 ggcctcgacc ggcgcggccc gcaagccatt ctcgatcttg gttgcggcac aggattgatg
     1801 ggcgtagcgg tgcgggattg ggccgatcgt ctcgacggcg tcgatctcag tccggcgatg
     1861 atcgagaagg cccgatcgcg cgcgatctac gatgaactgt ccgtgggcga tattcgcaat
     1921 tggctcgctg cgcgtgagta ccgttacgat ctggtcttcg ccgcggacac gctcgtctat
     1981 ctgggcgatc tcgggccgct gttcgaagtc gtcgcccgat gtctcgccca tgatgggcaa
     2041 ttcctcttca ctttcgaaaa gagctccggt ccgggtttcg agctgggacc aaagcggaga
     2101 tggcgccatt cggaaaccta t
//
LOCUS       DASZMC010000061         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3874513,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000061 DASZMC010000000
VERSION     DASZMC010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3874513"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..966)
                     /locus_tag="VG274_01170"
     CDS             complement(340..966)
                     /locus_tag="VG274_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWE05285.1"
                     /translation="MLTHKQIWTAIDALAARYNLSPSGLAKLAGLDPTTFNKSKRGDA
                     TGKLRWPSTESVSKVLAATGASLDEFVTLVEGIASRTRMVPLIGMAQAGSRGFFDDAG
                     FPVGSGWEEIAFPELADEHAYALEVTGDSMQPVYRDGDRIVVSPSTNVRRGDRVVVKT
                     QEGEVMAKLLHRMTAQRIELKSLNPAHEDRAFALNEIIFVHRIIWASQ"
     gene            1042..2088
                     /locus_tag="VG274_01175"
     CDS             1042..2088
                     /locus_tag="VG274_01175"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HWE05286.1"
                     /translation="MIDLAARVLRLLPAEPAHRATLSLASVFGPFLPAARASDPKLGI
                     DALGLHFPNPLGLAAGFDKDAEVPDAMLRFGFGFVECGTLTPRPQSGNPKPRLFRLRE
                     DRAVINRMGFNNGGMDVAARRLERRKRRGIVGINIGANKDSADRIADYALGFERLAPF
                     SDYVTVNVSSPNTPGLRGLQNRDELQQLLLTLTNARAQSGSRVPLLLKIAPDLDAQAM
                     ADIAQVVLNSGIEGLIVSNTTIARPATLRSMHAQEAGGLSGRPLLEPATRVLREMRRR
                     LDARITLVGVGGVSSGADAYAKIRAGASLVQLYTALAYEGPGLVERIRRELLECLVRD
                     GFSSVAAAVGADAR"
     gene            2182..2574
                     /locus_tag="VG274_01180"
     CDS             2182..2574
                     /locus_tag="VG274_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWE05287.1"
                     /translation="MNDDFGKLILRVAVGGLLLLHGVHKMLTGIAPIQAMVVAHHLPA
                     AVAYGVYLGEVVGPVMVILGLFARVGGALIVANMAMAVVLAGMGNLMTLNPMGGYALE
                     LEALYLFGGLSVMLLGAGRYSVMGGRWN"
     gene            complement(2571..>2938)
                     /locus_tag="VG274_01185"
     CDS             complement(2571..>2938)
                     /locus_tag="VG274_01185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWE05288.1"
                     /translation="VPYCAWLGMTARIEDGALILDLPFDGKLIGNPILPALHGGVTGS
                     LLETAAIVQILWETGGTALPKPVDITIDYLRSGRAVTSHARAWLARQGRRVVSARAEM
                     WQDDETKPVASFRGHFLLA"
BASE COUNT          519 a          865 c          943 g          611 t
ORIGIN      
        1 gccttcttcg atataggctt cgagcgcgcg attgttcgct tctacgacat ctcgtgcgcg
       61 cttcaactct ggaagcatcc acgatggtgc gggggcacct tgtgggatca tctgtaacac
      121 ggtgcgatac gttatcgccg cggcgggcga attcccctga agttcctccg acgaggcttt
      181 agaaagcaac gaccgtacgc gttgctccat gcccggaggc atatttgatc gttgttccat
      241 tatgatcgtg cttggtgatg caaagggatg atagcgatga aattactgca gtatccaccc
      301 aaaagcgtag ggaaatcacg aaatgtgact ttccggcaac tattggcttg cccagatgat
      361 gcgatggacg aagatgatct cgttcagcgc gaaggcgcgg tcttcgtgcg cgggattgag
      421 cgatttgagt tcgatgcgtt gcgccgtcat gcgatgcagg agcttcgcca tcacttcgcc
      481 ttcctgcgtc ttgacgacca cacggtcacc gcgtcggaca ttggtggacg gcgacaccac
      541 gattctgtcg ccgtcgcgat agaccggctg catggagtcg cccgtcactt ccagcgcata
      601 ggcgtgctcg tcggcgagtt cgggaaacgc gatctcctcc cagccgctgc cgaccgggaa
      661 tccggcgtcg tcgaaaaatc cgcgcgagcc cgcctgcgcc atgccgatca acggcaccat
      721 gcgcgtgcgc gacgcgatgc cttcgacgag cgtgacgaac tcgtcgaggc tggcgccggt
      781 cgcggcgaga accttcgaca cgctctcggt cgacggccag cgcagctttc ccgtggcgtc
      841 gccgcgcttg cttttgttaa aagtggtcgg atcgagaccg gcgagtttcg ccagtcccga
      901 aggcgagaga ttgtagcgcg cggcgagcgc gtcgatggcc gtccagattt gtttgtgtgt
      961 gagcatcggg gatgagaaat tgatccggtt tcacgggaat aattcctaga ataagttgag
     1021 gaattggtct agagggaaat gatgatcgat ctggctgcgc gcgttttgag gctcctcccg
     1081 gcggagcccg cccaccgcgc aaccctttct ctcgcctcgg ttttcgggcc cttcctccct
     1141 gccgcacgcg ccagcgatcc aaagctcgga atcgacgctt tgggtctgca ttttcccaac
     1201 cccctcggat tggcggccgg tttcgacaag gacgccgagg tgccggacgc gatgctgcga
     1261 ttcggcttcg gcttcgtcga atgcggcacg ctcacgccgc gcccgcaatc gggcaatccg
     1321 aaaccgcgcc tgtttcgcct gcgtgaggat cgcgccgtga tcaaccgcat gggcttcaac
     1381 aatggcggaa tggacgtcgc ggcgcgccgt ctcgaacgcc gcaagcggcg cgggatcgtc
     1441 ggcatcaata tcggcgcgaa caaagactcg gcggaccgca tcgcggatta tgcgcttggc
     1501 ttcgaacggc ttgcgccgtt ttccgattat gtgacggtga atgtctcgtc gcccaatacg
     1561 ccggggctgc gcggattgca gaatcgcgac gagttgcagc aactgcttct gacgctgacg
     1621 aatgcgcgcg cacagagcgg atcgcgcgtg ccactgttgc tcaagatcgc gcccgatctc
     1681 gatgcacagg cgatggccga tatcgctcag gtggttctga attccggaat cgagggtttg
     1741 atcgtgtcga atacgaccat cgcgcgtccg gcgacgctgc gaagcatgca tgcgcaagag
     1801 gccggcggtc tgtcgggcag acctttgctc gaaccagcga cgcgtgtgct tcgggagatg
     1861 cgtcgccgcc tcgacgcgcg cataacactc gttggcgtcg gcggcgtgtc cagtggcgcg
     1921 gacgcctatg caaaaatccg tgccggcgcg agcctcgtac agctctacac tgcgcttgcc
     1981 tatgaaggcc ccggtcttgt cgaacgcatc agacgcgaac ttctcgaatg ccttgtgcgc
     2041 gacggatttt cgagcgttgc cgctgcggtt ggcgccgacg cgcgctgaaa accggcttcg
     2101 ctttggtaaa cgcggtttaa gctgccgcaa cgggtgtttt tgagctagga tccttttgca
     2161 tatcgggtgc aggggggatt catgaacgac gattttggaa aattgattct gcgtgtggcc
     2221 gtcggcggcc ttctgctttt gcatggcgtg cacaagatgc tgaccgggat cgcgccgatc
     2281 caggcgatgg tggtggcgca tcaccttccg gcagcggtcg cgtatggcgt ttatctgggc
     2341 gaggtcgtgg gaccggtgat ggtgatcctg ggattgttcg cacgtgtcgg cggggcgctg
     2401 atcgtcgcaa acatggcgat ggccgtcgtg cttgcgggca tgggcaatct gatgacgctc
     2461 aatccgatgg gcgggtatgc gctcgaactt gaggcgctct atctgttcgg cggattgagc
     2521 gtgatgctgc tcggcgcggg gcgctacagc gtcatgggtg gacgctggaa ctaagccagc
     2581 aggaagtggc cgcggaaact ggcgacaggt ttcgtttcgt cgtcctgcca catctcggcc
     2641 cgtgcgctga cgacacggcg gccctgccgc gcgagccagg cacgcgcgtg gctcgtcacc
     2701 gcgcgtccgc tgcgcagata atcgatggtg atatcgaccg gcttgggcag cgcggtgccg
     2761 ccggtctccc aaaggatctg aacgatcgct gctgtctcca gcaacgagcc ggtcacaccg
     2821 ccatgcagcg ccggcaggat cgggtttccg atcagcttac catcgaacgg caagtccaga
     2881 atcagcgcgc catcctcgat ccgcgcggtc atgccgagcc acgcgcaata gggcaccg
//
LOCUS       DASZMC010000062         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2504953,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000062 DASZMC010000000
VERSION     DASZMC010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504953"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..763
                     /locus_tag="VG274_01190"
     CDS             26..763
                     /locus_tag="VG274_01190"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWE05289.1"
                     /translation="MGAAEAPAGETTVVLGAGWPGILLHEAVGHGLEADFNRKGTSAF
                     SGRVGQKVASELCTVIDDGTINSRRGSLNVDDEGAPTQKNVLIENGVLRGYLQDKLSS
                     RLMNQPATGNGRRESYQHIPMPRMTNTYMLAGASDPEEIIRSVEKGIYCTTFSGGQVD
                     ITSGNFVFSATESYLIENGKLTRPLRGASLIGNGPEALKYVSMVGTDLKLDEGIGVCG
                     KEGQSVPVGVGMPTVRIDRMTVGGTAA"
     gene            892..>1748
                     /locus_tag="VG274_01195"
     CDS             892..>1748
                     /locus_tag="VG274_01195"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWE05290.1"
                     /translation="MREVESVKEAGSRGAGLRVLVGQRTGSSYTSDLSPAGIATMVDA
                     ALSLAKITSEDPQAGLPEREMLGKLSGDLRLYDPAIEQLDTPLKIELAKRAEIAALDY
                     DPRIQNSEGAGFSSSHSRRVFANSRGFVGSYRTTSCGLSVSPIAKQDDSMERDYWYTA
                     SRFIASLESPEDVGKIAAQRSLRRLNPRKVPTQKAAVIFEPRTARAFLGDVFDAINGS
                     SIYRHASFLAGKLGEKVASESVTIVDDATIPGLFGTSPFDDEGVACRRTVVIDKGVLA
                     NYLLNTYTAR"
BASE COUNT          350 a          529 c          536 g          333 t
ORIGIN      
        1 agccgcacgc caggccatcg tgcaattggg cgcggccgaa gcgcctgcgg gtgagaccac
       61 cgtggtgctg ggcgcaggct ggcccggtat tttgctgcac gaagcggtgg gccacggcct
      121 ggaggccgat ttcaaccgca aaggcacgtc ggccttcagc ggacgtgtcg gccagaaagt
      181 ggccagcgaa ctttgcactg tgatcgacga cggcaccatc aacagccgcc gcggctcact
      241 caatgtggac gatgaaggcg cgcccacgca gaaaaatgtg ctgatcgaaa atggcgtcct
      301 gcgcggttat ttgcaggaca agctctccag ccgcctgatg aaccagcccg ccaccggcaa
      361 tggccgccgc gaaagttatc aacacatccc catgccgcgc atgaccaata cttatatgtt
      421 ggcgggcgct agcgatcccg aggaaatcat ccgctccgtg gagaaaggaa tttactgcac
      481 tacgttctct ggcggccagg tggatatcac cagcggcaac ttcgtgttct ccgcgacgga
      541 aagttatctg atcgaaaacg gcaaactcac gcggcctcta cgcggagcgt cgctcatcgg
      601 caatgggccc gaagcgctga agtacgtgag catggtgggc acggatctta agctcgatga
      661 aggcatcggc gtgtgcggca aggaagggca aagcgtgccg gtgggtgtcg gcatgcccac
      721 cgtgcgcatc gaccgcatga ccgtcggagg cacggctgca tgagtggcgc cgccgttgca
      781 gtcgatctgg aacagcttgc cgccgatgcc gttgatgccg cgcgaaagct gggcgctacg
      841 gacgccgaat gcactatctc cgagggcgag gaattctccg ttagcgtgcg catgcgcgaa
      901 gtggaaagtg tgaaggaggc cggttcacgc ggcgcggggt tgcgcgtact tgtgggccag
      961 cgcaccggct cgtcttacac gtcggatctt tcgcccgcgg gtatcgcgac gatggtcgac
     1021 gccgcgctct cgctcgcgaa aatcaccagt gaagatccgc aagccgggtt gccggaacgc
     1081 gagatgctcg gaaaactttc cggcgatctg cgcctctatg atcctgcgat cgaacagctt
     1141 gacacgccgc tcaaaatcga gctggcgaag cgcgccgaga tcgccgcgtt ggactacgat
     1201 ccacgtattc agaattcaga gggcgccgga ttttccagct cgcattcgcg acgcgtcttc
     1261 gccaattcgc gaggatttgt cggcagctat cgcactacaa gctgcggcct ttctgtttca
     1321 cccatcgcga aacaagacga ttcaatggag cgcgactatt ggtataccgc ctcgcgcttt
     1381 attgcgagtc tggaaagccc ggaagacgtc ggaaagatag ccgcccagcg ttccctgcgc
     1441 cgtctgaacc ctcgcaaggt tcccacgcag aaggctgccg tgatcttcga gccgcgcacc
     1501 gcgcgcgcct ttctcggcga tgtttttgac gccattaatg gctcttccat ttatcgccac
     1561 gcgtctttcc tggcgggcaa gctcggtgaa aaggtcgcct ccgagagcgt caccatcgtc
     1621 gatgacgcca ctattccggg tctgtttggc acgtctcctt tcgacgacga gggcgtcgcg
     1681 tgccgccgca cagtggtgat cgacaaaggt gttctggcca actacctgtt aaacacctac
     1741 acggcgcg
//
LOCUS       DASZMC010000063         5786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3596058,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000063 DASZMC010000000
VERSION     DASZMC010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3596058"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VG274_01200"
     CDS             complement(<1..213)
                     /locus_tag="VG274_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWE05291.1"
                     /translation="MDIEKFTERARGFIQSAQGLAVRSYHQTIGPLHLLKVLIDDEEG
                     LAARLIAASGGRPDIVRQEVESELGKV"
     gene            complement(348..734)
                     /locus_tag="VG274_01205"
     CDS             complement(348..734)
                     /locus_tag="VG274_01205"
                     /inference="COORDINATES: protein motif:HMM:NF025137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HWE05292.1"
                     /translation="MKRSRGRGRRPPQNNNPNRTFDSAGPEIKIRGSAAHVYEKYLQL
                     ARDANSAGDRVAAENYLQHAEHYYRIMAANQPQQPYQGQPHQTNGGSSVGNGRDTPLQ
                     NGSTPSFSLADAGRDLEDEESDDAAA"
     gene            complement(1000..1893)
                     /gene="prmC"
                     /locus_tag="VG274_01210"
     CDS             complement(1000..1893)
                     /gene="prmC"
                     /locus_tag="VG274_01210"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWE05293.1"
                     /translation="MNPAIREALRKGAQLLRAAGIESARLDARVLLARALGIEPDALL
                     TCESAGREGLGEFEALIFRRAMREPLAYITGTKEFWSLAFHVGTGVLIPRPETETLIE
                     EALRAHPERQAPLEVLDLGTGSGCLLIAFLVNYGRARGVGLDASEAALAYARRNACRH
                     GVIERCSFLNGDWPAAGEATFDVIFANVPYLSRSEVDESAPEIREHEPEAAVSAGNDG
                     LAAMRALAPVLARQLRKSGLAFVEIGAGQAVAVTRILGYCGLDVGHIASDLSGVPRCL
                     VVGRVGDVAGRRQRNQLEKGH"
     gene            complement(1890..2975)
                     /gene="prfA"
                     /locus_tag="VG274_01215"
     CDS             complement(1890..2975)
                     /gene="prfA"
                     /locus_tag="VG274_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWE05294.1"
                     /translation="MIPEAKLTRLVDRFAAIEAELAAGAGGRAYVKLSREYAELSPVV
                     EAIASYRDTERALRDAEAFAKDSAGDIELRTLAQEEQSALKDRFARMERELQLRLLPK
                     DAADSSSAIVEIRAGTGGDEAALFASDLLRMYQRYAQLMGWRTEILGLSDSDLGGVKE
                     AVIEVVGQGAYAKLKFESGVHRVQRVPVTEAGGRIHTSAATVAVLPEPEDVDVHVDEK
                     DLKIDVFRASGAGGQHVNKTESAVRITHLPSGIVVAQQDEKSQHKNRARALKILKAKL
                     FEAERARQASARAAERKGLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLERILSGD
                     ELSEIVDALVAEDQANRLAELEHEPDR"
     gene            complement(2987..4123)
                     /gene="ispG"
                     /locus_tag="VG274_01220"
                     /gene_synonym="gcpE"
     CDS             complement(2987..4123)
                     /gene="ispG"
                     /locus_tag="VG274_01220"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWE05295.1"
                     /translation="MSIRAYRDIARRKSRKIHVGAVEIGGDAPISVQSMTNTVTSDTG
                     ATIAQVRALEEAGADIVRVSCPDAESTSALKAIVRAAHVPIVADIHFHYKRALEAAAA
                     GAACLRINPGNIGSAQRVREVVAAAKDHGCSMRIGVNAGSLERDLLERYGEPCPEAMV
                     ESALNHARILEDNDFTEFKISVKASDVFLAVAAYQGLAEACDYPLHLGITEAGGLMSG
                     TVKSAIGMGMLLWSGIGDTIRVSLSADPVEEIRAGFDILKSLGLRHRGVNIVSCPSCA
                     RQGFNVIETVRTLEERLKHIATPMTLSIIGCVVNGPGEARETDLGFTGGGAGGNAGMI
                     YLNGKPAYKLDNAKLVDHIVELCEKKADEIDRERSAAATLEAAQ"
     gene            complement(4140..5228)
                     /locus_tag="VG274_01225"
     CDS             complement(4140..5228)
                     /locus_tag="VG274_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWE05296.1"
                     /translation="MTKVTHLTLDERGELSRRRVHLREISDDADAPLETVGQDLRAAR
                     LRRGNELAAVARALRIRKDHLEAIEEDRFEALPGRTYAVGFVRSYSAYLGLDSEQCVE
                     RFKKEIAGRADGAPQVGLAPETSESRLPRGWQVMAFVVIGVVAYGAYHLARSADRLSV
                     QTVAPVPARIATPEASAHKSPPNNRLAAQTHLVQKPAPTPNAAAVSQPVQQKAGGASL
                     GETRSAAPGAAQGSAKTPVVQSQPVPGKEAVSAEDKVAPGAGLSSGQELGARNARVVL
                     HVRGLTRILVEGADGKVFLNRILHPGDTYRVPNLVGLSLTTPDGGAVALELDGKDVGA
                     AGRSGEMTEALSLDPQAIVDRSGSRNPG"
BASE COUNT         1063 a         1909 c         1675 g         1139 t
ORIGIN      
        1 caccttgccg agttccgatt cgacctcctg acggacgata tctggacgac cgccggaagc
       61 agcgattagc cgcgccgcga ggccctcctc atcgtcaatc aataccttga gaagatgcag
      121 aggtccgatg gtctgatggt aagagcgaac ggcgaggcct tgggctgatt ggatgaaacc
      181 gcgcgcccgc tcggtgaatt tctcgatatc catgttgcct ctccggcagg gccccttcag
      241 gcagccactg caagccgcac ctcaatgcgc ggcccccgaa gggcgccgcg ctccttcaat
      301 atgtagaggg ctaatccggg cgaacaaggg cggttgggaa ggcgccgtca agcggctgcg
      361 tcgtcgctct cttcgtcttc caggtctctg ccggcgtcgg caagcgagaa tgacggggtg
      421 cttccattct gtagcggcgt atcgcgtcca ttgcccacac tcgatccgcc gttcgtctga
      481 tgcggctggc cctggtaggg ttgctgaggc tggttggccg ccatgatccg atagtagtgt
      541 tccgcgtgct gcagataatt ctcggcagcc acccgatctc ctgccgagtt cgcatcgcgc
      601 gcgagctgca gatacttttc gtacacatga gccgctgaac cgcggatctt gatctccggc
      661 ccggccgaat cgaatgtccg gttcggattg ttgttctgcg gcggccggcg gccgcgtcca
      721 cgtgagcgtt tcacctgatc ccctgtcctt tatgccaact cgtcatttcg tgcctgtttg
      781 catgtcgttc gcggtcaaag cccgttcccg cccattcgcg ccgccggggt tgcaccactg
      841 gcgcagatat gaccgtttgg aagcgcagta tgcgtctttc gccttgtaca ttgcacggcc
      901 agcggcgctt acgccgcgcc cgccgtcccc caaacgccgg atcggcccag cattcggcgg
      961 ggctccgtgc ttcccctact tccaagggaa gctagcgggt cagtggccct tttccaactg
     1021 gtttctttgg cgcctgccgg ccacatctcc cacccgtcca acaacgagac agcggggaac
     1081 cccagaaaga tcggaagcga tatggcctac gtcaagcccg cagtaaccga gaattcgcgt
     1141 caccgcaact gcctgtccgg cgccaatctc aacaaatgcc agtccgcttt tacgcagctg
     1201 ccgcgcaaga acgggtgcaa gtgcgcgcat tgccgccaga ccatcgtttc ccgcagagac
     1261 ggcggcttca ggctcgtgct ctctgatttc gggcgcactt tcatctacct ccgaccggga
     1321 aagataaggc acgttcgcga agatcacatc aaaagtggct tctcccgccg ctggccagtc
     1381 cccgttcaaa aagctgcaac gttcgatcac cccgtgccgg caagcgtttc ggcgggcgta
     1441 ggcaagcgca gcttccgacg catcgagtcc cacccccctt gcgcgtccat aattcacgag
     1501 aaaagcgatc agcagacacc ccgatcccgt tccgaggtcg agcacttcaa gcggcgcctg
     1561 gcgctcggga tgtgcccgga gagcctcttc aatcagtgtt tccgtttcag gacgaggaat
     1621 gagcacgccg gtacctacgt ggaatgcaag gctccagaat tcctttgtcc cggtgatgta
     1681 ggccagcggc tcgcgcatgg cgcgccggaa gatcaacgcc tcgaattcgc caagaccctc
     1741 ccggccggcg ctctcgcagg tcagcaatgc gtccggttcg atcccgaggg cgcgtgcaag
     1801 gagaacgcgt gcatcgagcc gcgcggattc aattccagcc gcgcggagca attgggcgcc
     1861 ttttctcagc gcttcgcgaa tcgcgggatt caccggtcag gctcgtgttc gagttccgcg
     1921 agccggttcg cctgatcctc cgcgacgagc gcatcaacga tctcgctcag ctcgtcgcct
     1981 gagagaatgc gctcgagctt gtacagcgtg agattgatgc ggtggtcggt cacgcggcct
     2041 tggggaaaat tgtaggtgcg gatgcgctcg gaacgatctc ccgagccgac gagacctttg
     2101 cgctcggccg ccctcgcgct cgcctgacgt gcgcgctccg cctcgaacag ctttgccttc
     2161 aatatcttga gcgctcttgc gcgattcttg tgctgcgatt tctcgtcctg ctgcgccacg
     2221 acgatcccgg aaggcaaatg cgtgatgcga actgcgcttt cagtcttgtt gacatgctga
     2281 ccgccggcgc cgcttgcgcg gaagacatcg atcttgaggt ccttttcgtc cacatgcaca
     2341 tccacatcct ccggctccgg aagcacggca accgtcgccg cagaggtgtg gatgcggcca
     2401 ccagcctcgg tgaccggaac gcgctgcacg cgatgcacgc cgctttcgaa cttcagcttg
     2461 gcataagcac cttggcccac gacctcgatg accgcctctt tgacgccgcc aagatcgctg
     2521 tcgctcaggc ccagaatttc ggtgcgccag cccatcaatt gcgcgtagcg ctgatacatg
     2581 cgcagaagat ccgacgcgaa caaagctgct tcatcgcccc cggtgccggc acggatttcg
     2641 acgatcgcag aggacgaatc ggccgcatcc ttcggcaaca atcgcaactg caattcacgc
     2701 tccatgcgcg cgaaccgatc cttcagtgcc gactgctcct cctgcgccag cgtgcgcagc
     2761 tcgatatcgc ccgcgctgtc tttcgcgaag gcttcggcat cacgcagcgc acgctctgta
     2821 tcgcgatatg acgcaatcgc ctcgacgacc ggcgacagct cggcatattc cctggagagc
     2881 ttcacatacg cccgcccgcc cgccccggca gcgagttctg cctctatcgc cgcaaagcgg
     2941 tcgaccagcc gcgtcagctt tgcctcggga atcatgcggg aatgcgctac tgcgcggctt
     3001 ccagggttgc cgcagcggag cgttcgcgat cgatctcgtc agcttttttc tcgcagagct
     3061 cgacgatgtg atcgacgagt ttcgcgttgt cgagcttgta ggccggcttg ccgttcagat
     3121 agatcatccc ggcattccct cccgctccac caccggtaaa gccgagatcg gtttcgcgcg
     3181 cttcgcccgg accgtttacc acgcacccaa tgatcgagag agtcatcggc gttgcgatgt
     3241 gcttcagccg ctcctcgagc gttcgcaccg tttcaatcac gttgaatccc tggcgtgcgc
     3301 aggaggggca ggacacgatg ttgactccgc ggtggcgaag tccgagcgac tttagaatgt
     3361 cgaagcccgc gcggatctcc tccacgggat cggcggaaag agacacccga atcgtgtcgc
     3421 cgatacccga ccataaaagc atgcccattc cgatggcgga tttgacggtg cccgacataa
     3481 ggccgccggc ctcggtgatt ccaagatgga gagggtaatc acacgcctcg gccaaaccct
     3541 gataggccgc aacggccaga aagacgtccg aggccttcac gctgatcttg aattcggtaa
     3601 agtcattgtc ttcgagaatg cgcgcgtggt tgagcgcact ctcgaccatc gcttccgggc
     3661 acggctcgcc gtaacgctcc agcagatccc tctcgagcga gcctgcattc acgccaatgc
     3721 gcatcgaaca gccatgatcc ttcgccgccg caacgacctc gcgcacgcgc tgcgccgagc
     3781 cgatattgcc gggattgatg cgcaggcatg ccgcgccggc cgcagccgct tcaagcgcgc
     3841 gcttataatg aaaatggata tcggccacga tgggaacgtg cgccgctctc acgatcgcct
     3901 tcagcgccga tgtggattcc gcatccggac acgagactcg aacgatatcg gctccggctt
     3961 cctccagcgc ccgcacttgc gcgatcgtcg caccagtgtc cgatgtcaca gtattcgtca
     4021 tgctctgaac ggaaatcggc gcatctccgc caatctcgac agcgccgaca tggattttgc
     4081 gcgacttgcg acgcgcaata tcgcggtagg ctctgatgct catggcgtca cctgaatggt
     4141 taaccgggat ttctcgatcc gctgcggtct actatcgcct gtggatcgag cgaaagcgct
     4201 tccgtcattt cgccggagcg gcccgcggct cccacgtcct ttccatcgag ttcgagtgcg
     4261 accgcgccgc cgtcgggggt cgtcagcgac agtcccacaa gattgggaac gcgataggtg
     4321 tcgccgggat gcaatatacg gttgaggaag accttcccgt cggcgccctc tacaaggatg
     4381 cgcgtaaggc ccctgacatg gagaacgacc cgcgcattcc gggcgcccag ctcttgccca
     4441 ctcgacaacc cggctcccgg cgcgaccttg tcttcggctg aaaccgcttc ttttccggga
     4501 acgggctggc tctggaccac aggcgttttc gctgaaccct gtgccgcacc tggggctgcg
     4561 gagcgggtct caccaagaga agctccaccg gctttctgct gcaccggttg tgacactgcc
     4621 gccgcattcg gcgtcggggc cggcttttgg accaaatggg tctgcgccgc gaggcggttg
     4681 ttcggaggag atttgtgggc actcgcttcc ggcgttgcga tcctcgctgg aaccggagcc
     4741 acggtttgaa cggacagccg gtcggccgac cgggcgagat gataagcgcc gtacgcgaca
     4801 acgccgatga cgacaaaagc catcacctgc cagccacgcg gcaatcgact ctcgctggtt
     4861 tccggcgcca atccaacctg cggtgctccg tctgcgcgcc ccgcgatttc ttttttgaag
     4921 cgctcgacac attgctcgga atcgaggccc agatacgctg agtagctccg gacgaaacca
     4981 acggcgtagg ttcgcccggg caatgcctcg aaacggtctt cctcgatcgc ttcaagatga
     5041 tccttgcgaa tccgcaacgc gcgcgccacg gccgcaagct cgttgccccg ccgcagacgc
     5101 gcggcccgca aatcctggcc caccgtttcg agcggcgcat cggcgtcgtc cgaaatctcg
     5161 cgcagatgta cccgccgtct gctcagttca ccgcgttcat ccagcgtcag atgtgtgacc
     5221 tttgtcatcc gcagacgaac tcgcccattc ggcttcaccc atcagcgaaa ccgtactggc
     5281 aggctagcgt caagtgcgac gacttcgctc gtcagtggct ccccccaagg gtattgagag
     5341 gctgctggat gccaagactt tgagctttca tcaaagtgtc ctgagactct tgctgacgcc
     5401 ggttccactc gcgggccgta tgacactcgc gcgccccgcc gagacgcgaa cccgtcatcg
     5461 ggggggtcat tcggcacaca acctcgtcgg aattagaggc ggaactcgcg gccgcgacag
     5521 gcacggctgg cgctgtctgg gccgtaacag tgggagccgg cactgtctgc gctgagggcg
     5581 cagaagccgc ctgtgccggg gcgggatccg ccgaggcgag agcgggcgcc ataatggtag
     5641 ccgcggaaac cagaaccaat gtcaaaacac gcatcaccac aaactccatc cggtatcgac
     5701 gcttccgcaa gatgcggtaa ctcgcgcctc atagcaaggg ccggttcaaa ctgccagatt
     5761 cttgcgtcta atgcttaatc acaatt
//
LOCUS       DASZMC010000064         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_25201,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000064 DASZMC010000000
VERSION     DASZMC010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25201"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..328
                     /locus_tag="VG274_01230"
     CDS             14..328
                     /locus_tag="VG274_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE05297.1"
                     /translation="MKKKPALRRSNRHSGSSLDSFLEEEEILEEVEAVAIKRVIAWQL
                     SEAMKKKGITKNTMATRLKTSRSQLDRLLDPDNAAVQLKTIARAARALGKRVSLVLKD
                     AA"
     gene            363..521
                     /locus_tag="VG274_01235"
     CDS             363..521
                     /locus_tag="VG274_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05298.1"
                     /translation="MDDATEPGEHGELGALARIRAWLGKKEPQHAAYMAPLRMRFGRQ
                     RNFIERLG"
     gene            484..1134
                     /locus_tag="VG274_01240"
     CDS             484..1134
                     /locus_tag="VG274_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05299.1"
                     /translation="MAASETSSSVSGEAVRRAEIEEQNRYLLAQQHQFRRAADIVTDA
                     WMAFAEVEAVAVIGSVAKALWKEVPRFRDFRREGTEVWHECKDLDLALWVDSQERLRD
                     LRRAAGRALSESYATGSGVSVAGNQLDIFLIEPGSNRYLGRLCKFNACPKGKRDCLVP
                     GCGTVPFNQRFEDFVPRADLLAPAGYATLYRRGMGRLRSALDLPAAGSSNVLWTVA"
     gene            complement(1349..1759)
                     /locus_tag="VG274_01245"
     CDS             complement(1349..1759)
                     /locus_tag="VG274_01245"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HWE05300.1"
                     /translation="MNDNDIERANEAARAQLAISPRAIGARYDRRRARIVVSLEKGVE
                     LAFPPQLAEDLGRAASSDLATIQISPSGLGLHWPKLDADLYLPALLQGVFGSPRWMAR
                     LLGRRGGRVRSKAKAAAARANGQRGGRPRKAQRG"
     gene            complement(1752..2000)
                     /locus_tag="VG274_01250"
     CDS             complement(1752..2000)
                     /locus_tag="VG274_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HWE05301.1"
                     /translation="MPTVLQFDGLRAVIYPNDHRPAHVHVIGPDKEAVFALNCPDGPS
                     RLSENYGFARRELRTISAALDLALESLCRKWETIHGRE"
     gene            2297..2653
                     /locus_tag="VG274_01255"
     CDS             2297..2653
                     /locus_tag="VG274_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWE05302.1"
                     /translation="MKYMFLLYADESGFTRLTPKEQSEGMAAYQAYTAALTRAGALVA
                     SNRLRPVATATTVRAKGSDFQVVDGPYAETREELGGFYMVDVPDLDAALNWAKQCPGA
                     RHGIVEVRPIWEMSQS"
     gene            2833..4080
                     /locus_tag="VG274_01260"
     CDS             2833..4080
                     /locus_tag="VG274_01260"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HWE05303.1"
                     /translation="MPVLGDDAHRAAEHAAREHYSQLLASLAARFRDVSAAEDALADS
                     LVAALAQWPISGVPERPAAWLLTVARHRHSDRLRRQTTAKAALDQLAFDAHVASGSPM
                     NDRRLELLFACAHPAIDENVRAPLMLQVVFGLDAGAIAGLFLISPAAMAQRLVRAKQK
                     IRDAHIPFHVPGPDAYPQRLDFVLQAIYALFTRDGALWHAATGNRDSLADEAIWLGDL
                     VTGLSNRHPEALGLLALMLFVESRRSARRDGERRYVPLSRQSVSLWDLAMIGRAETLL
                     REAADKNVMGKFQLEAAIQSAHCARAKTGATDWPAILALYDGILALGPTLVAEINRAL
                     AVLHVNDASAALSALDTISGARVEAYQPYWAARAHILEQCNRREDAIASYGRAIALEP
                     DEAAKDFLRDRRRALGDQGEERI"
BASE COUNT          796 a         1329 c         1281 g          701 t
ORIGIN      
        1 tgggtgaaag cgcatgaaga agaaacctgc gctccggcgc agtaacaggc acagcggctc
       61 aagcctcgac agctttctcg aagaggaaga aatcctcgaa gaggttgaag ccgtggcgat
      121 caagcgtgtg atcgcgtggc aactgtcgga agccatgaag aagaaaggca taacgaaaaa
      181 cacgatggcg acccggctga agaccagccg ttcgcaactc gatcgcctgc tcgatccgga
      241 caacgccgcc gtgcagctca agacgatcgc gcgcgcagcc agggccctgg gtaaacgcgt
      301 ttcgctggtg ctcaaggatg cggcgtagcg agcgggacag gcccaacctc gctgccaatg
      361 ccatggatga cgcaaccgaa cccggcgaac atggggagct tggcgcgctt gcccgcatcc
      421 gcgcctggct cgggaaaaag gaaccgcagc acgccgcgta catggcgccg ctgaggatgc
      481 gctttggccg ccagcgaaac ttcatcgagc gtctcgggtg aagccgtgcg ccgcgcggag
      541 atcgaggaac aaaaccgata tctgctcgcg caacagcatc agttccgcag ggccgccgat
      601 attgtaaccg atgcctggat ggcgttcgcg gaagtcgagg ccgtcgccgt gatcggctcg
      661 gtggccaaag cgctgtggaa ggaggttccc cgcttccgcg atttccggcg cgaaggcacc
      721 gaggtctggc acgaatgcaa ggacctcgat ctggccctgt gggtcgattc ccaggaacgg
      781 ctccgcgacc tacggcgtgc cgccgggagg gccttaagcg aatcctacgc tacgggatcg
      841 ggcgtcagcg tagccggcaa tcagctcgat atctttctga tcgaacccgg atccaatcgc
      901 tatctcggcc ggctttgcaa gttcaacgcc tgtcccaagg gcaagcgcga ctgtcttgtt
      961 cccggctgcg gtacggtccc gttcaaccag cgcttcgagg atttcgtgcc gcgcgccgat
     1021 cttctcgcgc cagccggata tgcgacgttg tacaggcgcg gaatggggcg attgcgcagc
     1081 gccctcgatc tgccggcagc cggaagcagc aatgtgttgt ggactgtcgc ttgataaatc
     1141 gacgcagacg accgcgaaac tttgacgcga gctgttagag cgatttccaa tcaaattgaa
     1201 tcacgcaccc aattgtcatt cccgccgaag cgggaatcca gcgcaagcga cgtgcggttg
     1261 ccctggatgc ccgcttcggc gggcatgaca gttttggttc aatatgacag gaatccgtcg
     1321 ctggcttcgt accacaacgc ttacgccctt accctcgctg ggccttgcgc gggcggccac
     1381 cccgttgccc gttggcgcgc gcggcggcgg ccttggcttt gctgcggacg cgtccgcccc
     1441 tgcgtccgag gagacgcgcc atccaccgcg gcgaaccgaa gacgccctgc agcaaagcgg
     1501 gcaaatagag atcggcatcg agcttcggcc aatgcagacc gagccccgac gggctgatct
     1561 ggatcgtagc gagatcggac gacgcggcgc gcccgagatc ttcggccaat tgcggaggaa
     1621 acgccagctc gacgcccttc tcgaggctca ccacgatgcg tgcgcggcgc cggtcgtagc
     1681 gcgctccaat cgcgcgcggg cttatggcca actgcgcacg ggctgcttcg tttgcccgct
     1741 cgatatcgtt atcattcacg gccatgtatc gtctcccatt tcctgcacag tgattccagc
     1801 gccaggtcga gcgcggccga tatggtccgc agctcgcgcc gcgcgaaccc gtaattctcc
     1861 gaaagtctcg aaggtccatc ggggcaattc agcgcaaaga ccgcctcctt gtccggcccg
     1921 atgacgtgaa cgtgtgccgg tctgtgatcg ttcggataga tcacggcacg caagccatcg
     1981 aactgcagaa cagtcggcat cccacgaaac tatcaaaacc caaccgcttt ggaaaggttc
     2041 cctccggggt tccttccggg gtcggagtac aattcggaga tgcccttgcc gcttccggag
     2101 gaaggctcgc cacgcgactg ggagtggaaa gcttggcacg tgaccggaaa tgaagcgcaa
     2161 acacatcggt cgtgcagcaa ccaccgcaaa aaaaattctc ttgctcccct ccccctgtcg
     2221 attcccctgc cctccgaacg acattaagcc ggcgcgggcg atgatcgctt gggcgccgca
     2281 accacgggag acccagatga aatacatgtt tcttctttac gccgacgaat ccggcttcac
     2341 ccgcctcacg ccgaaagagc agagcgaggg gatggcggct taccaggctt acaccgccgc
     2401 gctgaccagg gccggcgcgc tcgtcgccag caatcggctc aggccggtcg caaccgcaac
     2461 gaccgtgcgc gcgaagggca gcgatttcca ggtggtcgat ggtccctacg ccgaaacccg
     2521 ggaggaactg ggcggcttct acatggtcga cgttccggat ctggacgcgg cgctcaactg
     2581 ggcgaaacaa tgtcccggcg cgcgccacgg catcgtcgaa gtgcgcccga tctgggagat
     2641 gtcccaatcg tagcacttgg aatccatgat gtgtgacacg aaaacagact taacgcagag
     2701 ggcgctgtgg cagcgcagtg gacgcagaaa agtgttggcg cgccgcaggc gcgtgattgt
     2761 taccgtgcgt cctctgcgca tcctccgcgt acactgcgtt aaaaatctgc cgcatgggtc
     2821 agccaaggtc caatgccggt gctaggcgat gacgcgcatc gcgcggcgga acatgcggcg
     2881 cgcgaacatt acagccagct cctggcatcc ctggcggcgc gctttcgcga cgtaagcgcc
     2941 gccgaggatg cgctggcgga ttcgctggtc gccgcgctcg cgcaatggcc aatatcgggc
     3001 gtgcccgaac ggcctgccgc gtggcttctc accgtcgccc gccatcggca ttccgaccgc
     3061 ctgcgccggc aaaccactgc gaaggcggcg ctggaccaat tggctttcga cgcgcatgtc
     3121 gcaagcggat cgccgatgaa cgacagacgc ctcgagctgc ttttcgcctg cgcgcacccc
     3181 gcgattgacg aaaacgtgcg tgcgccgctg atgctgcagg tggtattcgg gctcgacgcg
     3241 ggcgcgatcg caggactctt tctgatttcg ccggcggcga tggcgcagcg cctggtccgc
     3301 gcgaagcaga agattcgcga cgcgcatatt ccctttcacg ttcccggacc cgacgcctat
     3361 ccccagcggc tcgatttcgt gcttcaggcg atctacgcgc tgttcacccg cgacggcgcg
     3421 ctctggcacg cagcgaccgg caaccgcgac agccttgccg atgaagcgat ctggctcggc
     3481 gatctggtca caggcttgag caaccgccat cccgaagcgc tggggctcct cgccctgatg
     3541 ctgttcgtgg aatcccgccg cagcgcgcgc cgcgacggcg agaggcgcta cgtgccgctc
     3601 tcgcggcaat ccgtgtcgct ctgggatttg gcgatgatcg gccgggccga gactctgttg
     3661 cgcgaagccg cggacaagaa cgtgatgggc aaattccagc tcgaagccgc cattcaatcc
     3721 gctcactgcg cgcgcgccaa aaccggagcg acggattggc ccgccatcct ggcgctctat
     3781 gacggaattc ttgcgctggg gccgacgctg gttgccgaga tcaaccgcgc cctcgccgtc
     3841 ctgcatgtca acgacgccag cgccgcactc tcggcactcg acacgatttc cggcgcccgc
     3901 gtagaagcct atcagcctta ctgggcggcg cgcgcgcaca tcctcgaaca atgcaaccgc
     3961 cgcgaagacg ccatcgcctc ctacggccgc gccatcgccc tcgagcccga cgaggcggca
     4021 aaggatttcc tgagggacag gcggcgcgcg cttggagatc agggagaaga aaggatttaa
     4081 cgcaatggac gcagaggatg cgcaggg
//
LOCUS       DASZMC010000065         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4162183,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000065 DASZMC010000000
VERSION     DASZMC010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4162183"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VG274_01265"
     CDS             <1..703
                     /locus_tag="VG274_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE05304.1"
                     /translation="CRSSSTMSELLHTLNAYSDLRRKLVIAADRPPQQLETLGLDVRS
                     RLSGGMVIALDKPERDTRLGILKARAEEYARKRGQTRIPDEALERIADLEDASPRDLI
                     GFFNNLTLHAELTKNPLAFSAAVDTIVRRGAVARKTSIEDIQRKIADFYKLDPRDFQS
                     AQRSRRVARPRQVAMFLAREITLRSLPEIGRRFGGRDHTTVLHACRRIVALCNEDPTF
                     KQEVDFLKQVLGKGG"
     gene            930..2054
                     /gene="dnaN"
                     /locus_tag="VG274_01270"
     CDS             930..2054
                     /gene="dnaN"
                     /locus_tag="VG274_01270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWE05305.1"
                     /translation="MKITVERSAFLKALSHVQSVVERRNTIPILSNVLIEAAKGRLKL
                     TATDLDMEIVESLTADVLRNGAATAPAHMLYDIVRKLPEGAQVQAELLTSEGGRLAVS
                     SGSVRFELACLPREDFPQMAAGAMPFRFRLQTDDLKKLIDKTRFAISTEETRFYLNGI
                     YVHAHRDGKAKGEMRAVATDGHRLARFQLDLPDGAAEMPGAIVPRKTIGELRRLLDDS
                     EGGIDVSLSDTKIQFTTDGVELTSKLIDGTFPDYQRVIPSANDKVLSLDSREFAQAVD
                     RVSTISADKTRAVKLGIVKDKLTLTVINPDSGTATEEVGASYSASPLDIGFNARYLLD
                     ITNQIEGKEIRFLLSDAGSPTLIEDSEDRGTLYVLMPMRV"
     gene            2093..3250
                     /gene="recF"
                     /locus_tag="VG274_01275"
     CDS             2093..3250
                     /gene="recF"
                     /locus_tag="VG274_01275"
                     /inference="COORDINATES: protein motif:HMM:NF000820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HWE05306.1"
                     /translation="MPDRRHLAVTRLMLTNFRSYAHAELSVSGRPVVLAGPNGAGKTN
                     LLDAISLLSPGRGMRGALLAEHIRKAPQTARNDVLWAVAASVSRDSETYEIGTGLVSS
                     AQGNERRQVHLNGVPAQSSAELGEIVEMAWLTPAMDRLFSDSASARRRFLDRLVLGFD
                     AAHGRRTLRYEQAMRERARLLKLGPRDSGWLDGLENEMAETGIEIAAARAATVKRLNA
                     SLFERGEAGAFPSAQLALAGHTDRILAEESGRAVESLRIALAGARLRDAEARRTTVGP
                     HVSDLSARHTSKRMDARDCSTGEQKALLISIVLADARELAQQRGGGAPILLLDEIAAH
                     LDRQRRAALFEEILAVGAQAWLTGTDLALFEDLTSRADIFLVEASELVRQN"
     gene            3298..>4120
                     /locus_tag="VG274_01280"
     CDS             3298..>4120
                     /locus_tag="VG274_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWE05307.1"
                     /translation="MAFVPEPADPNAYGADQIKVLKGLDAVRKRPGMYIGDTDDGSGL
                     HHMVYEVVDNAIDETLAGHADRIDVVLNADGSCAVRDNGRGIPTDIHEEGVSAAEVIM
                     TQLHAGGKFEQSAYKVSGGLHGVGVSVVNALSEWLDLRIWRDGREFYMRFRDGVPEAP
                     LRVVGEAQGRRGTEVTFLPSPKTFTRLEFDSSTIEHRLRELAFLNSGVTIILADLRGV
                     QKKEVTLHYDGGLEAFVRYLDRAKTPLIPSPVMLRSERETIIVEAALVWNDSYHES"
BASE COUNT          789 a         1287 c         1319 g          725 t
ORIGIN      
        1 gtgccgcagc agttccacca tgtctgaatt gctgcatacg ctgaatgcct attccgacct
       61 gcggcgcaag ctcgtgattg cagcagacag gccgccgcag cagctcgaga ctttgggtct
      121 ggacgttcgt tcgcggctct ccggcggcat ggtgattgca ctggacaagc ccgagcggga
      181 tacgcggctc ggcattctca aggcgcgcgc ggaggaatat gcgcgcaaac gtgggcagac
      241 caggattccg gacgaggctc tggagcggat tgcggatctg gaggatgcca gtccgcgcga
      301 cctgatcggg tttttcaaca atctcacgct gcacgcggag ctgacgaaaa acccgctggc
      361 cttcagcgcg gcggtcgaca ccatcgtcag gcgcggtgcg gttgcgcgca agacttcgat
      421 cgaggacatt cagcgcaaga ttgcggattt ctacaagctc gatccgcgcg attttcagtc
      481 cgcccagcga agccggcgcg ttgcacggcc gcggcaggtg gcgatgttcc tggcacgtga
      541 aatcacgttg cgttcgcttc ccgagatcgg ccgccgcttc ggcggacgcg atcacacgac
      601 cgtgctccat gcctgccggc gtatcgtggc cctgtgcaac gaagacccga ccttcaagca
      661 ggaggtcgac tttctgaagc aggttctggg caagggaggc tgatcccccc tgggtgtcaa
      721 ctggcctcct ggggcgacgc accggggggc ctatttcctg tccggccgca cggttgcgct
      781 gttctggcag gatgcgccct gttatactct cggccttgag tcccactccg tcgggaattc
      841 aggcaaatca agggctttcg gtcaaccggg gctcccatcc ggaagcccgc gggcacgtcg
      901 aaaacagcga gagtttcaag cgcaaaatca tgaaaatcac ggtcgaacgc agcgcctttc
      961 tgaaggcact cagccacgtc caaagcgtcg tggaacgccg caacaccatt ccgattctat
     1021 cgaatgttct gatcgaggcg gcaaaaggcc ggctgaaact gaccgccacc gatctcgaca
     1081 tggaaatcgt ggaatcgctg accgccgatg tgcttcgcaa cggcgccgcg acggcgccgg
     1141 cgcacatgct ttacgacatc gtgcgaaaat tgccggaagg cgcgcaagtc caagcggagc
     1201 ttctcacgtc ggaaggcggc cgtcttgccg tttcgtccgg aagcgtgcgc ttcgaactgg
     1261 cctgcctgcc gcgggaagac tttccgcaga tggcggcggg cgcaatgcca ttcaggtttc
     1321 gtctgcagac cgacgatctg aaaaagctga tcgacaagac ccgctttgcc atatcgaccg
     1381 aggagacgcg attctatctc aacggcatct atgtgcacgc gcaccgggac ggcaaggcaa
     1441 agggtgaaat gcgcgcggtt gcaaccgatg gccacaggct cgcgcgattc cagctggacc
     1501 tgccggacgg cgccgccgag atgccgggcg caatcgtgcc gcgcaagacg atcggcgagc
     1561 tgaggcgtct gctcgacgat tccgaaggag gcatcgacgt ttcgctgtcg gatacgaaga
     1621 tccagttcac gaccgacggc gtggaactga cctccaagct gatcgacggc acgtttcccg
     1681 attaccagcg cgtgatcccg tcggcgaacg acaaggtcct gtccctcgat tcgcgggaat
     1741 tcgcccaggc ggtggatcgc gtgtccacga tttccgcgga caaaacccgc gccgtgaagc
     1801 tcggcatcgt caaggacaag cttaccctta cggtgatcaa tcccgattcg ggtaccgcca
     1861 cggaagaggt gggcgcctcc tacagcgcga gcccgctcga catcggattc aacgcccgct
     1921 acctgctcga catcacgaac cagatagagg gcaaggaaat acgcttcttg ctgtcggacg
     1981 cgggctcgcc gacgctgatc gaggattccg aggaccgggg cacgctttac gtcctcatgc
     2041 ccatgcgggt gtgagtttga ctcacgcgct tcgccaatca tcggctgcaa ccatgccgga
     2101 tcgccgtcat ctcgcggtca ccaggctgat gctcacgaat tttcgttcct acgcgcatgc
     2161 ggagctttcc gtgagcggac ggccggtggt gctcgcgggc ccgaacggcg ccggcaagac
     2221 gaacctgctc gacgcaattt cccttttgtc cccgggccgg ggaatgcggg gcgcattgct
     2281 tgccgaacac atccgcaagg ctccgcagac ggcgcggaac gatgtgctgt gggcggtcgc
     2341 cgcttcggtt tcgcgggaca gcgaaaccta cgagatcggc accgggcttg tctcttctgc
     2401 gcagggcaac gaaaggcggc aggtccatct gaacggcgtg ccggcgcaga gttccgcgga
     2461 gctcggcgaa atcgtcgaga tggcctggct gacgcccgcg atggacaggc tgttcagcga
     2521 cagcgcgagc gccagacggc gatttctgga tcgtctggtg ctgggcttcg acgcggcaca
     2581 tgggcggcgc acgttgcgct atgagcaggc gatgcgcgaa cgcgcgaggc tccttaagct
     2641 cggcccccgc gattccggct ggctcgatgg gttggaaaat gagatggccg agaccggaat
     2701 cgaaattgca gcggcgcgtg cggcgaccgt caagcgtctc aatgcttcgc tttttgagcg
     2761 tggcgaggcc ggcgcatttc ccagcgcaca acttgcactt gccggccaca cggaccgtat
     2821 cctggcggag gagagcgggc gtgcagtgga atccctgcgg atcgcactcg cgggcgcgcg
     2881 cctgcgcgat gccgaagcgc gacggacgac cgtgggtccg cacgtcagcg acctttccgc
     2941 ccggcacaca agcaaaagaa tggatgcgcg ggattgctcg accggcgagc aaaaggcgct
     3001 tctgatctcg atcgtgcttg ccgacgcgcg cgagcttgcg cagcagcgag gcggcggcgc
     3061 cccgatactc ctgctcgacg aaatcgcggc ccatctggat cggcaacgca gggccgctct
     3121 gttcgaggaa atcctggcgg tcggcgcgca ggcatggctc accggcacgg atctcgcgct
     3181 gtttgaagat ctgacttcgc gtgccgatat cttccttgtc gaagcgagcg agctcgtccg
     3241 ccagaactag aattcgcgaa cgggcgacgg caattgcaga aagagcacga tccaccaatg
     3301 gcctttgtac ccgaaccagc cgatcccaac gcctacggcg ccgatcagat caaggtgctg
     3361 aaagggctcg acgcggtgcg caagcgcccg gggatgtata tcggcgacac cgatgacgga
     3421 tctggcctcc accacatggt ctacgaggtg gtcgacaatg ccatcgacga gactctggcg
     3481 gggcacgcgg accggatcga cgtggtgctg aacgcggacg gttcctgcgc ggtgcgcgac
     3541 aacggccgcg gcatcccgac cgacatccac gaggaaggcg tttcggccgc cgaagtgatc
     3601 atgacccagc tgcatgcggg cggaaaattc gagcagagcg cctacaaggt ctcgggtggc
     3661 ctgcacggcg tcggcgtctc ggtcgtcaac gcgctgtccg aatggctcga tctgcgcatc
     3721 tggcgcgacg gccgggaatt ctacatgcgc ttccgcgacg gggttcccga agcgccgctg
     3781 cgggtggtcg gcgaggcgca aggccgccgc ggcacggaag tgacattcct gcccagtccg
     3841 aagacgttca ccaggctcga gttcgacagc tccacgatcg agcatcggct gcgcgagctt
     3901 gcatttctca attccggcgt gacgatcatt ctcgccgatc tgcgcggagt ccagaagaag
     3961 gaggtgaccc tccactacga cggaggcctc gaagcgttcg tccggtatct cgatcgcgcc
     4021 aagacgccgc tgatcccgtc gcccgtcatg ctccgctccg aacgcgaaac catcatcgtc
     4081 gaagcagctc tcgtgtggaa cgactcctat catgagagca
//
LOCUS       DASZMC010000066         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4976498,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000066 DASZMC010000000
VERSION     DASZMC010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4976498"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VG274_01285"
     CDS             complement(<1..1161)
                     /locus_tag="VG274_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWE05308.1"
                     /translation="MTASRVTAVLGPTNTGKTHFAIERMLAHKSGMIGLPLRLLAREV
                     YDKIVRIKGVKLAALITGEEKIVPPDAKYFVCTVEAMPLDLGTAFVAIDEIQLCADPE
                     RGHVFTDRLLNARGEEETLFMGADTMRGAIQRFVPRTYFISRARFSDLAYTGHKKLTR
                     LPRRSAVVGFSAEDVYGIAELIRRQRGGAAVVLGALSPRTRNAQVELYQNGDVDFLVA
                     TDAIGMGINMDVDHVAFAAMEKFDGVGVRPLRPEEVGQIAGRAGRHMNDGTFGVTAEA
                     ESLDEELVARVEGHRYDPVRVLQWRNSALNFHSLDALIASLDELPPAKGLMKARPSSD
                     LIALRHLSQMDDVRELTRGTAGVRRLWDVCQLPDFRKLSTDEHARLVHTIFLF"
     gene            complement(1265..>1998)
                     /locus_tag="VG274_01290"
     CDS             complement(1265..>1998)
                     /locus_tag="VG274_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE05309.1"
                     /translation="VQETSWNWSKAAEAHAQLLARYPKNADAHHFYGYVMMNLYLPER
                     WLDEHRKAAALDPLSALDQENIGEALHVLGRNDEAIVEYQKALTLDPNLVFTLAQLCV
                     SYAERGTVGDAKKILMERLIAVDGDGNHTTRCRAAIAGREPDPKKALRALAQDAERAN
                     QAGKVNESLVGLIYAQAGDFDSAFKWFRKAIAIHDSRFFQNTIGNLLPAALIADPRWK
                     ALFDGPALQEWRQARAAVLSGPLDK"
BASE COUNT          356 a          618 c          638 g          386 t
ORIGIN      
        1 aaacaggaag atcgtgtgga cgagcctggc gtgttcgtcg gtactcagct tgcgaaaatc
       61 cggaagctgg cagacgtccc acagacgccg cacgccggcc gttccgcgtg tcagttcgcg
      121 cacatcgtcc atctggctga gatggcggag cgcgatgaga tcgctggacg gccgcgcctt
      181 catcaggccc ttcgcaggcg gcaactcgtc caggctcgcg atcaaggcgt cgagcgaatg
      241 gaagttgagc gccgagtttc gccattgcag tacacgcaca gggtcgtagc ggtggccttc
      301 gacacgcgcg acgagttctt cgtccagcga ctcggcttcc gccgtgacgc cgaatgtgcc
      361 gtcgttcatg tgtcggccgg cgcggcccgc gatctggccg acctcttcgg gcctgagtgg
      421 acggacgccg acgccgtcga acttctccat cgcggcgaac gcgacatgat cgacatccat
      481 gttgatgccc atgccgatgg cgtcggtcgc gacgaggaaa tcgacatcgc cgttctggta
      541 gagctcgacc tgcgcgttgc gcgtgcgtgg cgacagcgct cccagaacca ccgctgcgcc
      601 gccgcgctgg cggcggatca gctccgcgat gccgtacaca tcctccgcgg agaagcccac
      661 gacggccgaa cggcgcggca ggcgtgtgag tttcttgtgt ccggtgtagg cgagatcgga
      721 gaagcgtgcg cgggagatga aataggtgcg cggcacgaaa cgctggatcg cgccacgcat
      781 cgtgtcggcg cccatgaaca gcgtctcttc ctcgccgcgc gcgttcaaca gacgatctgt
      841 gaagacgtgg ccgcgctcgg gatcggcaca gagctggatc tcatcgatgg caacgaacgc
      901 ggtgccaaga tcgagcggca tcgcctcgac ggtgcagacg aaatatttcg catccggcgg
      961 aacgatcttc tcctcgccgg tgatcagcgc ggcgagcttc acgcctttaa tccgcacgat
     1021 cttgtcatag acttcgcgcg ccagaaggcg cagcggcaac ccgatcatgc cggatttgtg
     1081 cgccagcatc cgctcgatcg cgaaatgggt cttgccggtg ttggtggggc cgaggaccgc
     1141 ggtgacgcgg gacgccgtca atggtcttgt cgccccaacg gacgcgcgtg ctactttcat
     1201 gatgctctga atattgggtg tttcgtccgg ttcggcaacc ggcgcaaagc ccggtcgcga
     1261 gcgtctattt gtccagtgga ccggacaaga ccgcggcgcg cgcctgccgc cattcctgca
     1321 acgcggggcc atcaaacaac gctttccagc ggggatccgc gatcagtgcc gccggaagca
     1381 agttcccgat cgtattctga aagaaccgcg aatcatggat ggcgatggcc ttgcgaaacc
     1441 atttaaaggc gctgtcgaaa tcccccgcct gcgcatagat gaggcctacc agactctcgt
     1501 tcactttgcc tgcctggttg gcgcgttctg catcttgcgc cagggcgcgc agcgctttct
     1561 ttgggtcggg ttcccgcccc gcgattgctg cgcggcagcg cgtcgtgtgg ttgccgtcgc
     1621 cgtccacggc gatcagacgc tccatcagga ttttcttcgc gtcaccgacc gtgccgcgct
     1681 ccgcatacga aacgcagagc tgcgccagcg tgaacacgag attggggtcg agtgtgaggg
     1741 ctttttgata ttcgacaatg gcctcgtcgt tgcggccaag gacatgcagc gcctcgccga
     1801 tgttttcctg atcgagcgcg gaaagcggat cgagcgcggc cgccttgcgg tgctcgtcga
     1861 gccagcgctc gggcaaataa agattcatca tgacgtagcc atagaaatga tgtgcgtcgg
     1921 cattcttggg gtaccgcgcc agaagttgcg catgggcttc cgcagctttg gaccagttcc
     1981 aggacgtttc ctgaacct
//
LOCUS       DASZMC010000067         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_26445,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000067 DASZMC010000000
VERSION     DASZMC010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26445"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VG274_01295"
     CDS             complement(<1..945)
                     /locus_tag="VG274_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HWE05310.1"
                     /translation="MTAGFQRTTPALLAYRFATTALAPVARLVLRARARTGKEDMARL
                     SERFGRASKARPPGGLVWIHGASVGECISALPLIDRLLEFFGRAVLVTSGTVTSAQLM
                     QQRLPERALHQYVPIDTPSAVARFLDHWRPDAALFIDSELWPNLLLSARERGVRLALI
                     NARMSARAFAGWRRAPRTARRVLGCFETCLVQDEASAARFASLGASDVRVCGNLKADA
                     RPESPDPARLAALEHAIAGRPILLAASTHPGEDETILPAHDALRERYPALLTIIAPRH
                     PARGTEIAMLCGSRAALRRSDGCLPRAETAVYVADTIGE"
     gene            complement(942..2816)
                     /locus_tag="VG274_01300"
     CDS             complement(942..2816)
                     /locus_tag="VG274_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE05311.1"
                     /translation="MSTYPHASDQAAAETALRLPGDRARPADAHDFAIIQRLLRDYMG
                     RRWNRLALAAACMLTTAGMSGVLAWLLDPATRQLFLENNPRMLLIIPLAIVGVVALRA
                     ATAFGEQYVLNNVAERVVADVQRDMFSSQVRLDIATLNEIHSGELVSKFLYDATLLRG
                     AITRGVAGLGKEFVTLVVLAAVMIYEDWQLALISVVLLPLVAWVTQRLSRSLRKSSTR
                     GMEETGTLSRALSEALAGRRIVKAYSLESYATRSAESRIAQRLKYILRAVRARAAAVP
                     STDLIGGLAAAATVAFAGYQHLHGHLAINQFFAFTGAMLLAQQPVRTLSQLWTISTEG
                     LSAANRIFALIDTKPRISDRRDARPLVMRPPPNGGFIRLEDVSFSYREEGTAALNQIS
                     LDIPSGRKVALVGPSGAGKSTIFNLLLRFYDIDEGAIRIDGQDIGTVTLESLRANIAL
                     VTQEPFLFDETIADNIALGRRDASREEIVASARAAAADGFISELPRGYDSRIGEGGLR
                     LSGGQRQRIAIARAMLRNAPILLLDEATSALDAESERQVRDALARLMEGRTTVVIAHR
                     LSTVLDADRIYVLDQGRLAEWGTHGELVARGGLYARLYKHNLAEETGNEAACTAAVPG
                     "
     gene            complement(2898..3137)
                     /locus_tag="VG274_01305"
     CDS             complement(2898..3137)
                     /locus_tag="VG274_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HWE05312.1"
                     /translation="MPERESKQLLHLVFGGEVVTPDSVEFRDTSKLHVVGIYPDYNEA
                     YSAWRAAAQATVDNAHMRYFVVHIHRLLDPGVAVR"
     gene            complement(3146..>3683)
                     /locus_tag="VG274_01310"
     CDS             complement(3146..>3683)
                     /locus_tag="VG274_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HWE05313.1"
                     /translation="GTTAFLKERPHFSISVAVAEHGRSVAGVVYNPILDELFAASEGD
                     GARLNAAPIRVSDCGAIEGCRVLGAKEMFSHPPWSEPPNSPWPEMQVESRSSIAYRMS
                     LVASGRFDAAIVLAPKHDWDMAAGDVIVREAGGGVTAMDGRCLQFNGPTTIQRSMICA
                     GPKLHALLIERLRHSASA"
BASE COUNT          697 a         1139 c         1219 g          628 t
ORIGIN      
        1 ctctccgatc gtatcggcga cgtagacagc ggtctccgcc cggggcaggc agccgtccga
       61 gcgccgaagg gctgcgcggg aaccgcaaag catcgcaatt tcggttcccc gcgctggatg
      121 tcgcggagca atgattgtga ggagggctgg gtatcgctcg cgcagcgcgt catgtgcggg
      181 gaggatcgtt tcatcttcgc ccgggtgcgt gctggccgcc aacagaattg gtcggccggc
      241 gatcgcatgt tcgagcgcag ccagtctggc cgggtctgga gattcggggc gagcgtcggc
      301 cttgagattg ccgcacacgc gcacatcgct cgcgcccagg ctggcaaaac gcgcggcgct
      361 tgcttcgtcc tggaccagac aggtttcgaa gcaacccagg acgcgccgcg ctgttctggg
      421 ggcgcgccgc caacccgcga aagcgcgcgc cgacatccgt gcattgatca gcgctagccg
      481 cacgccacgc tcgcgagccg acagcagaag attgggccat agttccgaat caatgaagag
      541 ggcagcgtcg ggcctccagt gatcgaggaa tcttgcgacc gccgaaggtg tgtcgatggg
      601 cacgtattga tgcagcgcgc gctcaggcag gcgctgctgc atcagctgtg ccgaggtaac
      661 cgtgccgctc gtcaccagca ccgcgcggcc aaaaaactcg agaagcctgt cgatgagcgg
      721 cagcgccgaa atgcattcgc cgacgctggc gccgtgaatc cagacaagcc cgccgggcgg
      781 ccgcgccttg ctggcgcggc caaagcgttc tgacaggcga gccatatcct ctttgccggt
      841 gcgtgcgcgc gcgcgcagga cgagacgggc gactggcgcc agcgccgttg tcgcgaagcg
      901 ataggcgagc aaagcaggcg tcgtacgctg gaatccggcc gtcatccggg gacagccgcg
      961 gtgcacgctg cctcgttgcc tgtctcctca gccagattgt gtttgtacag gcgtgcgtag
     1021 agcccgccgc gcgccacgag ctcgccatgt gtgccccact cggcgagccg gccctgatcg
     1081 agaacataga tgcggtccgc atccagcaca gtcgacaggc ggtgggcgat gacaacggtt
     1141 gtacggcctt ccatgagacg ggcaagcgcg tcccgaacct ggcgctcgct ttccgcatcg
     1201 agcgccgagg tcgcttcgtc gaggagaagg atcggggcat tgcgcagcat ggcgcgggcg
     1261 atcgcgatgc gctggcgctg tccgccggaa agccgcagac cgccttcgcc aatgcgagag
     1321 tcgtagcccc gtggcagttc ggaaataaat ccgtccgctg cagccgcgcg ggcagaagca
     1381 acgatttctt cgcgcgatgc atctcgcctg ccaagggcga tattgtcggc gatggtctca
     1441 tcgaaaagga agggctcctg cgtgacgaga gcaatgtttg cgcgcaggga ttcgagcgtc
     1501 accgttccga tgtcctgacc atcgatgcga atggcccctt cgtcgatatc gtagaaccgc
     1561 aagagaagat tgaagatcgt gctcttgccg gcgcccgaag gaccgaccag cgccactttc
     1621 cgcccggaag ggatatcgag cgaaatttga ttgagcgcgg cggtgccttc ctcgcgatag
     1681 ctgaaggata cgtcctccag gcggatgaag ccgccgttag gaggggggcg catgacgagc
     1741 ggtctcgcgt ccctgcgatc cgatattctc ggctttgtat cgatcagggc aaatatacgg
     1801 ttggccgcgg aaagtccttc ggtcgagatg gtccagagct ggctcaatgt gcgcaccggt
     1861 tgctgggcaa gcaacatcgc tccggtgaag gcaaagaact gattgatggc gagatgacca
     1921 tgcagatgct gatagccggc aaaagccacg gtagcggcgg ccgcgagtcc gccgatcagg
     1981 tcggtcgacg gcacggcagc tgcgcgcgcc ctgaccgcac ggagaatgta tttcagacgt
     2041 tgggcgatgc gggactcggc cgagcgtgtc gcataagatt cgagcgagta ggccttgacg
     2101 attcgccggc cggccaatgc ttccgagagc gcgcgggaaa gcgtgccggt ttcctccatg
     2161 ccgcgcgtcg aggatttgcg aagcgacctg ctgaggcgct gtgtcaccca ggccacgagc
     2221 ggcagcaaaa cgacggagat cagcgcaagc tgccagtctt cgtagatcat caccgcggca
     2281 agcacgacga gcgtgacgaa ttccttgccg aggcctgcca cgccgcgcgt gatcgcgccc
     2341 cggagcaaag tcgcgtcata gagaaatttc gagaccagtt ctcccgaatg gatttcattg
     2401 agcgtggcga tgtcgaggcg gacctggctc gaaaacatgt cgcgctgaac gtccgcgacg
     2461 acgcgctcgg cgacgttgtt gagaacgtac tgctcgccga acgcggtcgc cgcgcgcagc
     2521 gcgacgacgc cgacgatggc gagcgggatg atcagcagca ttctcggatt gttctcgagg
     2581 aagagctgcc tcgtcgcggg atcgagcagc catgcgagca cgccgctcat ccccgcagtc
     2641 gtgagcatgc acgctgccgc aagggcgagc cggttccacc ggcggcccat gtagtcgcgc
     2701 aatagccgct ggatgatggc gaaatcgtgc gcatctgccg ggcgcgcgcg gtcgccgggc
     2761 aagcgcagcg ccgtctctgc ggcggcctga tccgatgcat gcgggtaagt gctcacgcct
     2821 gctctggcta tgctggatga attccggggc ccacgcgaca ggttagcatg gtctaccgcc
     2881 agtgaggaaa ggcgcgctca cctcactgcc acgccagggt ccagcagtct gtgaatatga
     2941 accacgaaat agcgcatatg ggcattgtcg accgtagcct gcgcggccgc ccgccacgcg
     3001 gaataggctt cattgtagtc gggatagatg ccaacaacgt ggagcttcga agtatccctg
     3061 aattcgacgc tatccggcgt cacgacctcg ccgccaaaga caaggtggag caactgtttc
     3121 gattcgcgtt ccggcatcgg tgtctctagg ctgaagcgct gtggcggagg cgctcgatca
     3181 gcagagcgtg aagtttcgga cccgcgcaga tcatcgaccg ctggatcgtg gtcggaccat
     3241 tgaattgcag gcatcgtccg tccatcgccg tgacaccgcc gcccgcttcg cgcacgatca
     3301 catcgcccgc cgccatgtcc caatcgtgct tgggggcaag cacgatcgcg gcgtcgaacc
     3361 ggcctgacgc gacgagcgac atccgatagg cgatggagct tcgcgattca acctgcatct
     3421 cgggccatgg cgagttcggg ggttccgacc agggcggatg ggaaaacatc tctttcgcgc
     3481 ccagtacacg gcaaccctcg atggcgccgc aatcgctgac ccggatcggt gcggcgttca
     3541 gccgtgcgcc gtcgccttcc gaggcagcga agagttcgtc gagaatgggg ttgtacacga
     3601 caccggccac cgaccgcccg tgttccgcca ccgccaccga aatcgagaaa tgcggccgct
     3661 ctttcagaaa cgccgtggtg ccg
//
LOCUS       DASZMC010000068         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4163960,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000068 DASZMC010000000
VERSION     DASZMC010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4163960"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1986)
                     /locus_tag="VG274_01315"
     CDS             complement(<1..1986)
                     /locus_tag="VG274_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWE05314.1"
                     /translation="MRARVFLVGAIAAGLAPAARGQEAQGIETVTVIAVAPMPGTGID
                     ANKIAGETQTLSMSDLARDRPQDAVPNAVATRLSSVSLGGEEGSPFQPDFIFRGFHAS
                     PVSGSAEGLAVYQDGVRLNESFGDTVNWDLIPEFAVDRFTVQSNNPVFGLNALGGAVT
                     LTMKNGLNFEGTDVDLSGGSFGNLTGHAEYGARFGKLGIYFGIGGVEDDGFRYHSPTA
                     LRQAYGDLAWQNGRLALHLSASGALDDIGALGPTPVQLLAQDPRAVFTWPQSTENGME
                     LVQLRGSWRASDTLSLSANTYVRHFIQHLVDGNTTDVATCTNDPAQLCLEGSDLYPRD
                     ALYDAQGKPVPTSALPPGATPGETDFTRTDTTSEGAALQGSLTLPIPGHDNTLVAGAS
                     LDRGATNYAAQGELGTLMSNLEIAGSGVIIDQSLSPTAQPPIEAPVNVDATNTATGVY
                     ALDVFDLTSQLSWTLSGRLNIAQIGLRDRLGGALTGDHAYTHFNPGTGLAYKLSGSLT
                     AYAGYSESNRAPTAGELSCSNPVSPCLLGAFLVSDPALKQVVSRNIEAGLRGSFASAM
                     LPGRFSWSAGAYRTDAQDDIQLLATSINGFGFFSNAGTTRHQGVDLHLGYLDRSLRID
                     ASYSWLDAIFLNSENLSSNSPAANAQGLIHVAPGDRLP"
BASE COUNT          365 a          630 c          674 g          357 t
ORIGIN      
        1 cggcaggcga tcgccgggcg cgacgtgaat gaggccttgt gcgttcgccg ccggactgtt
       61 ggacgagagg ttttcggaat tgagaaatat ggcatcgagc caggaatagc tcgcgtcgat
      121 gcgcaagctg cggtcgagat aacccagatg cagatcgacg ccctggtgac gcgtcgtccc
      181 cgcattgctg aaaaaaccga agccgttaat gcttgtcgcg agcagttgaa tatcgtcctg
      241 ggcgtccgtc ctgtacgcgc cggcgctcca ggaaaagcgt ccgggcagca tggcggaggc
      301 gaagctgccg cgcagtccgg cctcgatgtt gcgcgacacc acctgcttca gggcgggatc
      361 cgaaacgagg aatgcgccga gcaggcaagg cgagacggga ttggagcacg acaattcgcc
      421 cgcggtcggt gcacggttcg attcggaata gcccgcatag gccgtcaggc tcccggagag
      481 cttataggcg aggccggtgc ccggattgaa atgcgtgtaa gcgtgatcgc cggtgagcgc
      541 gccgcccaac ctgtcccgca atcctatctg cgcgatgttg agccggccgc tcaatgtcca
      601 ggacaattgc gaggtcagat cgaacacgtc gagggcgtag acgcccgtgg cagtgttggt
      661 cgcatccaca ttcaccggcg cttcgatcgg cggctgcgcg gtcgggctca ggctttgatc
      721 gatgatgacg cccgaaccgg cgatttcgag atttgacatg agcgtgccga gctcgccctg
      781 cgctgcgtaa ttggtcgcgc cgcgatcgag cgacgcgccc gctacgagcg tgttgtcgtg
      841 tccgggaatg ggcagggtga gcgagccctg cagcgcggcg ccctcgctcg tcgtgtcggt
      901 gcgggtgaaa tccgtctcgc cgggcgtcgc gccgggcggc agcgcgctcg tgggcaccgg
      961 cttgccttgc gcatcgtaga gggcatcgcg cgggtagaga tcgctgccct ccagacagag
     1021 ttgcgcgggg tcgttcgtgc aagtggcgac atcggtcgta ttgccgtcga cgagatgctg
     1081 gatgaaatgt cggacatagg tgttcgcgct gagcgacagg gtgtcgcttg cgcgccagct
     1141 tccgcgcagc tgaacaagct ccatcccgtt ctctgtcgac tgcggccagg tgaagaccgc
     1201 ccgcggatcc tgcgccagca actgcacggg cgtggggccc agcgcaccga tgtcgtcaag
     1261 cgcccccgag gcggagaggt gcaacgcaag cctgccgttc tgccaggcga gatcgccata
     1321 ggcttgcctg agcgcagtcg gcgaatgata gcggaagccg tcatcctcga cgccaccgat
     1381 cccgaaatag atgccaagct tgccgaacct cgctccatat tcggcgtgac cggtcagatt
     1441 gccgaaggag ccgccggaca gatccacgtc ggtgccttcg aagttcaatc cgttcttcat
     1501 cgtgagcgtg accgcgccgc ccagcgcgtt gagcccgaac acgggattgt tgctttgcac
     1561 cgtgaagcgg tcgaccgcga attccggtat caaatcccag ttgacggtgt cgccgaagga
     1621 ttcgttcagc cgcacgccat cctgatatac ggcgagccct tcggcgctgc cggaaacggg
     1681 ggacgcatgg aagccgcgaa agatgaaatc cggctgaaac ggacttccct cctctccgcc
     1741 aaggctgacg ctggacaggc gcgtggcgac cgcgttgggt actgcatctt gcgggcggtc
     1801 ccgcgcaagg tcggacatgg agagcgtttg agtttctccg gcgatcttgt tcgcatcgat
     1861 ccccgttccg ggcatcggcg ccaccgcgat cactgtgacc gtttcgatac cctgcgcctc
     1921 ctggccgcga gcggcgggcg cgagaccggc cgcaatcgcg cccacgagaa aaacccgcgc
     1981 cctcacccgg acacctgcgc gccgcgaacc aagaagcatg tccctg
//
LOCUS       DASZMC010000069         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_852263,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000069 DASZMC010000000
VERSION     DASZMC010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852263"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VG274_01320"
     CDS             complement(<1..620)
                     /locus_tag="VG274_01320"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWE05315.1"
                     /translation="MFAPDMYSTTLPRDRSSDVGYAHANFVLLAAALTISVVQLFAAP
                     LILPINAWTAFAIVALCAVSTPLHWGLMHESIHGNLFANPSANRGTGRISGALLCLSW
                     DVMRFGHLLHHSANRHELDRPEAVPPGGSRFSAAGPYFMKLLGGHALISAMSAMAFAL
                     PARSVPRLVGKFASGDGLAPIRIAAVRAFTNRERQARIRFDLVLIF"
     gene            complement(1502..>2746)
                     /locus_tag="VG274_01325"
     CDS             complement(1502..>2746)
                     /locus_tag="VG274_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWE05316.1"
                     /translation="AEARAQREITAADQWPSLSGNASYANQRISTTTAQGSLFGAFGK
                     AGGGGLPVNIPSFPNPYNQYQIGFDASWEPDLFGSVRRSVEAAQADSQASEEDAHDAL
                     VSLEGEVARAYIDLRSAQAQLAITNRNLATERDVFELASQRRSAGLATDLDATNAAAQ
                     VRQTESLLPPLEQTVASDINQLSQLLAREPGALHDELRTAKPVPPIPAEVRIGLPGDL
                     VRRRPDIRAAEARLHAATARVGVAVAALFPNVTFNANFGTQAERFPDLAEWASRFYSI
                     GPSLEVPIFEGGRLRATVRLADLKEKEAALDYARIVLNAVHEVENALVAYGSEQSRRA
                     ALAATLVQNRKALGLARQRYRSGVTAFLDVLDAERTAQQTELSLAVSTAAISVDLVAL
                     YKALGGGWGPNEDVQIAKQAEP"
BASE COUNT          509 a          874 c          895 g          468 t
ORIGIN      
        1 aaaaaaatca ggacaagatc gaagcggatc cgggcctgac gttcgcggtt cgtgaaggcg
       61 cgaacagcgg cgatgcgtat cggcgcaagc ccgtcacccg atgcaaattt cccgaccagc
      121 cgcggcaccg aacgcgccgg caatgcgaac gccatcgcag acatcgccga tatcagcgcg
      181 tgtccgccga gcagcttcat aaaatagggg ccggcggctg agaagcggga gccgccggga
      241 ggcaccgctt ccggccggtc caattcatga cggttggcgc tgtgatgcag aagatggccg
      301 aagcgcatca cgtcccacga caggcacagc aaggcaccgg agattcgccc tgtaccgcga
      361 ttcgccgatg gatttgcgaa caggtttccg tgaatcgact cgtgcatgag cccccagtgc
      421 aacggcgtgg agaccgcaca aagggcgacg attgcgaacg ccgtccatgc gttgatcggc
      481 aggatcagcg gggcggcgaa caattggacc acagatatcg tcaatgccgc ggcgagcagc
      541 acgaaattgg catgcgcata cccgacatcc gaagaacggt cgcgcggcag cgtcgtggaa
      601 tacatatctg gagcaaacaa gcggaatcgt cccagccggg ctacgcagaa ttatccatgc
      661 tgaatggttg cgagcaagat aattcccgtc atgcaatccc caatgtgcga tgtcgagtcc
      721 aaggatcgcg ccatcgctcc agccgacgat cgccgctttg ccgatatgca gatagtcgag
      781 aagccccagc acgtccgagg ccatgagggc ataaccgaac ggcgcggagt tgcgggagct
      841 gcggccgtgt ccgcggctgt ccatcacgat gaccgtgtag tgccgcgcca gcgccggcac
      901 ctgatttccc caatagttcg cgttcgccag tccgccgtgc agaaggacga caggggcgcc
      961 cgcgccgaac tccgcgtacc agatcctgat cccgttcacg ggcgccaatc cgctgcgttc
     1021 cgaatgcgga agcacaggcg tcggcggcag cgccatccat tgcggcgcgg cctgcgctgg
     1081 aaatgtccag gagaggagaa gaggcgcgag cgccccgcac attcggcgcg agtgtgattt
     1141 tcctcgcccg ttggcggcta gcgttggcac gcattgtttc atagttcagt cgctaccaag
     1201 catggcccct gttttcctcc cccatcgtgg gggaggtgcc gagccccccg gtccgcgcga
     1261 agcgcggccg aaggataaac tccgcgaggc ggtgggggag tggcgcgcgc gccgcgcgcg
     1321 gactcccctc tccggtttgc tgcgcttcgc tccgcaaacc acctccccca cgttggggga
     1381 ggaaaaccag gtgcgcagca ctcggctcaa attcagtgtt gacggcctcg gtcggattca
     1441 taaaacaatg tgtgccaacg ccagccgcaa gcgggggagg aaagaggctt gccgataatg
     1501 cttacggctc ggcttgcttt gcaatctgca catcctcgtt cggtccccat ccgcctccaa
     1561 gcgctttgta gagggcgacg agatcgacgg atatggcggc ggtgctgacg gcgagggaga
     1621 gctccgtctg ctgcgccgtc cgctccgcgt cgaggacgtc gaggaatgcc gtcactccgc
     1681 ttcggtagcg ctgccgcgcg agcccgagcg ctttgcggtt ttgcacaagt gtcgcggcca
     1741 gggcggcgcg ccggctctgt tcgctcccat aggcgacaag ggcgttttca acttcgtgca
     1801 cggcattgag cacgatgcgc gcatagtcga gcgccgcttc cttctccttg agatcggcaa
     1861 ggcgcacggt cgcgcgcagt cggccgcctt cgaagatcgg cacttcgagg ctgggtccga
     1921 tcgagtagaa gcggctggcc cattcggcga gatcgggaaa gcgctccgcc tgcgttccga
     1981 agttcgcatt gaaggtgacg ttcggaaaaa gcgcggcaac ggcaacgccg acgcgcgccg
     2041 tggctgcgtg caggcgcgcc tcggcggcgc ggatgtccgg ccggcgccgg accagatcgc
     2101 cgggaagacc aattcgcacc tccgccggaa tcggcggaac gggcttcgcc gtccgcagct
     2161 catcgtgcaa cgcgcccggc tcgcgagcca ggagctggct cagctgattg atgtccgacg
     2221 caacggtctg ttcgagcggc ggcaacagcg actcggtttg ccgcacctgt gctgccgcgt
     2281 tcgtcgcatc gaggtcggtg gccagccccg ccgagcggcg ctggctggcg agttcgaaca
     2341 cgtcgcgctc ggttgcgaga ttgcgattcg tgatcgccaa ctgcgcctgg gcgctcctga
     2401 ggtcgatata ggcgcgcgcc acttcgccct cgagcgagac gagcgcatca tgcgcatcct
     2461 cctccgatgc ctgtgaatcg gcctgcgccg cttccacgga gcgccgcacg ctgccgaaca
     2521 aatcgggttc ccatgaagca tcgaagccga tctggtattg attgtacggg ttgggaaagc
     2581 tgggaatatt gacgggcaat cctccgccgc cggccttgcc gaacgcgccg aacagcgaac
     2641 cctgcgccgt cgtcgtgctg atgcgctgat tggcatagga ggcgttcccg gacaggctcg
     2701 gccattgatc cgctgcggtg atctcgcgtt gcgcgcgcgc ttccgc
//
LOCUS       DASZMC010000070         8016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4165252,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000070 DASZMC010000000
VERSION     DASZMC010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4165252"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..614
                     /locus_tag="VG274_01330"
     CDS             273..614
                     /locus_tag="VG274_01330"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWE05317.1"
                     /translation="MKLLLDENLSRRIVPFLQELFPESSQVTLLGLERAKDRDIWEFA
                     KTNDFVIVTSDADFEELSLLLGAPPHVIWLRGGNSSKSETLNLLTSHEEMIRESLREG
                     RACVEIEKDIA"
     gene            complement(712..1266)
                     /locus_tag="VG274_01335"
     CDS             complement(712..1266)
                     /locus_tag="VG274_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE05318.1"
                     /translation="MSGNDPSIAVAVARLRKERKLSLAALAQLAGISRSMLHEIEKDR
                     TNPTVGIAWRLATALRVDLPELLRGSVQSPIVDVIGEHATPWLTSEDGLCRLRILGPL
                     QLAGHLEWYLLDAEPKGELVSSAHQPRTTEHLSVLKGELVVSVDNEDSIVPAGATIRY
                     RADRPHAIRNRSKAPAEALLMVVL"
     gene            1339..2385
                     /gene="tdh"
                     /locus_tag="VG274_01340"
     CDS             1339..2385
                     /gene="tdh"
                     /locus_tag="VG274_01340"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HWE05319.1"
                     /translation="MFAILKARPEQGLSHADVENPKGPGPGEVEVAVSMAGVCGTDFH
                     IWKWDEWSSRRVKPPMVVGHEFVGRIARLGEGVHHFSPGQRVSAECHVTCGHCRLCRT
                     GRAHLCRETSIIGVDRAGAFAERVVVPAGNAWAVDNAIPDRHAAVFDPCGNAMHAATI
                     VPLAGRDVLITGAGTIGLFSIGIARSHGARRVMVTEPSPYRARLARELGADLVADPRA
                     ADTRAQILAMTDGQGPEIVLEMSGNAGALRSALEVASNGAHVVLLGLPASDVTLDLAE
                     TVIMKGATLHGVTGRRMFGTWYDVSAFMLRNPDVMDTILTHRLAARDYAEGFDLIAAG
                     NCGKVLLDFAGKAA"
     gene            2382..3587
                     /locus_tag="VG274_01345"
     CDS             2382..3587
                     /locus_tag="VG274_01345"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HWE05320.1"
                     /translation="MSNPVLQRLAGELEKAREIGNYKTLHELETATKAHVVVANIGEV
                     LLMCSNDYQGFAAHPAVVAAARTALERYGVGPASVRFICGTLMIHEQLERKIAEFLHA
                     EAAITYSSCWSANTGLFPVLAGPGDVVLSDALNHASIIDGCRMVSKDVVRDVYKHGDM
                     NDLERVLHQHADRRGKIIVTDGVFSMEGDLAPLPEIVRLAKSHNALVVVDDSHGVGVI
                     GPSARGTVEHYGLLDRVDIHTGTLGKALGGGAGGYVAGPRIVVDSLIQRSRPHLFSNA
                     VPPSVAAAALEAIKLLEQEPERAGKLRAKAELFRRTLKSKGITPLEGEGAIVPVVVGQ
                     SVKSKAISQDLLAGGLLATSFSFPVVPEGAARIRFQVSQSHSEADLEWAAQLIADTFH
                     KHGATGAVG"
     gene            complement(3595..3981)
                     /locus_tag="VG274_01350"
     CDS             complement(3595..3981)
                     /locus_tag="VG274_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05321.1"
                     /translation="MRTVLATASGAALLLMLAPQAFGASAVVVGPPSADPIEQAGIMN
                     SARAFTIANDRQASIVRFRRGAGNAICNRVDYWGVNGAAPIPLHVEWNGRAATVMPGR
                     CLRINAPSALVAAAAPMSSDTILQGT"
     gene            4344..5267
                     /locus_tag="VG274_01355"
     CDS             4344..5267
                     /locus_tag="VG274_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWE05322.1"
                     /translation="MSGPEHFVSIVTWVVIGFVVLVFLSATFFTVEQQSRAIIERFGR
                     YIRTASPGLNVKVPLIDGIAQRVSLRVQQLPVEVETKTLDNVFVKLFVAVQYRVVEGQ
                     EADSYYKLQNHVDQITSYVLDVVRAKVPKMNLDEVFEKKDEVGTSVKTELDASMKIYG
                     FEIPNALVTDVNPADNVKAAMNEIQTQQRLQVAAAAKGEANKILIVKSAEAEAESKRL
                     QGEGIAKQRRAIVDGLRESIAAFTDKIGDVTAPEVLKLVLMTQYFDTMKEIGVSSGSK
                     VILMPHTPGGMSDIAEQLRAAIISANETTKA"
     gene            complement(5236..5628)
                     /locus_tag="VG274_01360"
     CDS             complement(5236..5628)
                     /locus_tag="VG274_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05323.1"
                     /translation="MKLAVDTNVLVRYLTWDDETQARAARDHIESADAIAVSTVVLCE
                     TVWVLKRAYRYSGAEIAETLAGVIHSRNVETDQPAAGAGLRMLARGGDFADGVIEHEA
                     IRAGCDRLATFDETFEKLLMPSLSRSRR"
     gene            complement(5625..5867)
                     /locus_tag="VG274_01365"
     CDS             complement(5625..5867)
                     /locus_tag="VG274_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWE05324.1"
                     /translation="MSELTITAKGQVTLKRAVLEHLGIGPGGKVRVSLLPGGRVTLAP
                     AAAAADIRQLRGALRRRVRRAVSLEQMQKAIESGGR"
     gene            6173..>8016
                     /locus_tag="VG274_01370"
     CDS             6173..>8016
                     /locus_tag="VG274_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HWE05325.1"
                     /translation="MAQPAQTETPPPSDRPWMNPTLEPDARAALLEAQMTQDEKLRLV
                     LGFFGVKGGSIFARGAPPEYQAGLRNTAGFIPGIPRLGIPALVESDAGVGIANSANTR
                     PGDEATALPSGLAMAATWNPDLVFQGGAALGAEARDRGYSVVLAGAMNLARDPRGGRT
                     FEYAGEDPLLAGTVAGAEVAGVQSAHVVSTVKHFAFNDQETGRRELSAVIGEAEARES
                     DLLAFEIAIERGSPGAVMCAYNRLNGVYSCENPFLLTDVLKRDWKFPGWVLSDWGAVH
                     STVESANAGLDQESASGFDRQLYFAAPFAQALADGSVPPARLDDMVHRILRTMFARGI
                     IDNPPAPRLSDIAGHLAIAQLEAEDGIVLLRNEGALLPLKSHPRSIAVIGGHADIGVL
                     SGGGSSQVMPIGHSLANEYPVGGPVIVLPGGARAMPVSGYVFDPYSPLAAIETAAPEA
                     RITYANGEDIAVAVAAAKNADVAIVFALQWMTEGSDVPNLSLPGTQNALIAAVAAANP
                     RTIVVLETGGPVLMPWLANVPAVVEAWYAGNGGGDAIANVLFGEVNPSGRLPVTFPAS
                     EDQLPRPVLPGPDTPGAWFDVDYVEGADVGYRWFEKKKLVPLFPFGFGL"
BASE COUNT         1425 a         2587 c         2590 g         1414 t
ORIGIN      
        1 atacgctaga ttcgcgcagg tttgaaatcc gatacagagg tctcgatcat gattgagacg
       61 gacagaatta cactggagcc gggaaaacgt ggggggcaac cgacaattcg cgggctgcga
      121 atcactgttt atgatgtgct ccgtcaactc gcgaatggcg cgagcgaagg ggacattctg
      181 gctgattttc ccgaactcga aagtgctgat atccgcgcat gtctcgctta cgccgcggat
      241 cgcgagcgca agacagtcac gctgcgcgca acgtgaagct gctgcttgac gagaaccttt
      301 cgcggcggat tgtgccgttc ctgcaagaac tctttccgga aagttcgcaa gttactctcc
      361 tgggactcga gcgggcgaag gatcgcgaca tctgggaatt cgccaagacc aatgatttcg
      421 tgattgtcac gagcgacgcc gacttcgaag agcttagtct gcttctgggc gcgccgccgc
      481 acgtaatctg gttgagaggc ggaaattcat caaaatcaga aacgttgaat ttgctgacgt
      541 cacatgagga gatgatccgc gaaagcctga gggaaggacg cgcgtgcgtg gagatcgaga
      601 aagacatcgc gtaaaccctg cgcgaaagtg atatccgtcc ccgcctggcg gccgagcagc
      661 aattcacacc acttcatcgt tccctacctt tcagttctgc gtgtcgcaag tttagagcac
      721 caccatcagc agcgcttcgg cgggcgcctt gctgcggttg cggatggcgt gggggcggtc
      781 ggcgcggtag cggatcgtgg cgccggcggg gacgatgctg tcttcgttgt cgacggacac
      841 gaccagttcg cccttgagca cggagagatg ttcggtggtg cgcggctggt gcgcggacga
      901 gacgagttcg cccttcggct cggcatcgag cagataccat tcgagatgcc cggcgagttg
      961 aagcggcccg agaatccgca gccggcagag cccgtcctcg ctggtcagcc acggcgtcgc
     1021 gtgttcgccg atcacgtcga cgatcgggct ctgcaccgag ccgcgcagca gttccggcag
     1081 atccactcgc agcgctgtcg ccagccgcca ggcgatgccg accgtcggat tggtgcggtc
     1141 tttctcgatc tcgtgcagca tcgagcgcga aatgccggcc agttgcgcca gcgccgcgag
     1201 cgacagcttg cgctccttcc tgagccgggc gacggcgacg gcaatgctgg gatcgttccc
     1261 ggacatgtcc actatcttgg ttgcgccgta ccaatatagc ggataaatag gccgcaacga
     1321 aaacagagtg gggacgccat gttcgccatc ctcaaagccc gccccgaaca ggggctttcg
     1381 catgccgacg tggaaaaccc gaaggggccc ggccccggcg aggtggaagt cgccgtcagc
     1441 atggcggggg tgtgcggcac cgattttcac atctggaaat gggacgaatg gtcgtccagg
     1501 cgggtgaagc cgcccatggt ggtgggccat gaattcgtcg gccgcatcgc ccgtttgggc
     1561 gaaggcgtgc accatttctc gccgggccag cgggtgagcg ccgagtgcca tgtgacgtgc
     1621 ggacattgcc ggctctgccg caccgggcgc gcgcatctct gccgcgagac ctcgatcatc
     1681 ggcgtcgacc gcgccggcgc cttcgccgaa cgcgtggtgg tgccggccgg caatgcgtgg
     1741 gcagtggaca acgcaatccc tgaccggcac gctgcggtgt tcgacccctg cggcaacgcg
     1801 atgcacgcgg cgaccatcgt gccgctcgcc ggacgcgacg tgctcatcac cggcgcggga
     1861 acgatcggcc tgttcagcat cggcatcgcg cgctcgcacg gggcgcgtcg cgtgatggtg
     1921 accgagccct cgccctaccg tgcgcggctg gcgcgggaac tgggcgccga tctggttgcc
     1981 gatccgcgcg ctgccgacac gcgcgcgcaa atccttgcca tgaccgacgg ccagggtccc
     2041 gagatcgtcc tcgaaatgag cggcaatgcg ggagcgttgc gcagcgcgct cgaagtggcg
     2101 agcaacggcg cccatgtggt gttgctcggc ctgcccgctt ccgatgtgac gctcgatctc
     2161 gccgagacgg tgatcatgaa aggcgcgacg ctgcacggcg tcaccgggcg gcgcatgttc
     2221 ggcacatggt acgacgtctc ggccttcatg ctgcgcaatc ccgatgtgat ggacacgatc
     2281 ctgacccatc gcctcgcggc ccgggattac gcggaaggct tcgacctgat cgccgccggc
     2341 aattgcggca aggttcttct cgattttgca gggaaagcgg catgagcaat ccggttcttc
     2401 aacggctcgc gggcgagctc gaaaaggccc gcgaaatcgg caattacaag acgctgcacg
     2461 agctcgagac ggcgacgaaa gcgcatgtgg tcgtcgccaa tatcggcgaa gtcctgctga
     2521 tgtgctccaa cgactatcag ggctttgccg cgcatcccgc ggtggtcgcc gccgcgcgca
     2581 cggcgctgga gcgttacggc gtcggcccgg cttccgttcg gttcatctgc ggcacgctca
     2641 tgattcacga acagctcgag cgcaagatcg cggagttcct gcacgcggaa gccgcgatta
     2701 cctattcctc ctgctggtcc gccaacacgg gattgtttcc cgttctcgcc gggccgggcg
     2761 acgtcgtgct ctccgacgcg ctcaatcatg cgagcatcat cgatggctgc cgcatggtgt
     2821 cgaaagacgt cgtgcgcgac gtctacaaac acggcgacat gaacgatctc gaacgcgttc
     2881 tccaccagca tgccgaccgg cgcggcaaga tcatcgtcac cgacggcgtc ttctcgatgg
     2941 aaggcgatct ggcgcccctg cccgagattg tgcggctcgc gaaatcgcac aacgcgcttg
     3001 tggtggtcga cgattcccac ggcgtcggcg tgatcgggcc atcggcgcgc ggaacggtcg
     3061 agcattatgg ccttctcgac cgcgtcgata tccataccgg cacgctcggc aaggcactgg
     3121 gcggcggcgc gggcggctat gtcgcgggcc cgcgaattgt cgtcgattcc ctgatccagc
     3181 gttcgcgccc gcatctgttc tccaacgctg tgccgccttc cgtcgccgcc gccgcgctcg
     3241 aagcgatcaa gctgctcgag caggagccgg agcgcgcggg caaactgcgc gcgaaagcag
     3301 aactcttccg ccgcacgttg aaatcgaaag gcatcacccc gctcgaaggc gaaggcgcga
     3361 tcgtgccggt cgttgtcggc cagtcggtga aatccaaggc gatcagccag gatctgctgg
     3421 ccggcggatt gctggcgacg agcttttcat tcccggtggt gcccgaaggc gccgcgcgaa
     3481 tccgcttcca ggtctcgcaa tcgcacagcg aggcggatct cgaatgggcc gcgcaactta
     3541 tcgccgacac cttccacaaa cacggcgcga cgggcgcagt cggatagacg ctgcttacgt
     3601 gccttgcaga atcgtatccg acgacatcgg cgccgcggcc gcgacgagcg cgctcggtgc
     3661 gttgatgcgc aggcaccgtc ccggcatcac cgttgcggcg cgcccgttcc attccacgtg
     3721 cagcggaatc ggcgccgcgc cgttcacgcc ccaatagtcg acgcggttgc agatcgcatt
     3781 tccggcgccg cgacgaaagc ggacgatcga cgcctggcgg tcgttggcga tggtaaaggc
     3841 acgggcggaa ttcatgatgc ccgcctgctc gatcggatcg gcgcttggcg gcccgacaac
     3901 taccgccgaa gcaccgaacg cctgcggggc gagcatcagc agcaaagctg caccagacgc
     3961 tgtcgcaagg acggttctca tgggctgctc ctatgttatt ccggcgctgc ccttgcccgc
     4021 tccctctgga aacgaatgca gagcagaccc gttgcgaagt gggacgcaag cggcgcgcac
     4081 gcacagcgca tttcctcccc cgctgttcgc gggggaggtg ccccgcacaa gcgggagcgg
     4141 aggtgggtaa gtagcggcac gctccccaca taccccctcc ggcgcgacgc ttcgctcgcg
     4201 ccaccttgcg aagagcgggg aggaaagtgg cggaggaaag cgaaacgcgg gtgaggggtg
     4261 atgcgagcga cgggtcggca atagaatcct tacaccctgt acgctactct tgcgcgcaaa
     4321 catttcaccg aaggggcagc atcatgtcgg ggccggaaca cttcgtctcc atcgttacct
     4381 gggtcgtcat aggcttcgtc gttctcgtct tcctctccgc cactttcttc acggtcgagc
     4441 agcagagccg ggcgatcatc gagcgcttcg ggcgctatat ccgcaccgct tcgcccggcc
     4501 tcaatgtgaa ggtgccgctg atcgacggga tcgcccagcg cgtgtcgctg cgcgtgcagc
     4561 agctgcccgt cgaagtggag accaagacgc tcgacaacgt gttcgtgaaa ctgttcgtcg
     4621 cggtgcagta ccgcgtggtc gagggacagg aggcggattc ctattacaag ctgcagaacc
     4681 acgtcgacca gatcacgtcc tacgtgctcg acgtcgtccg cgccaaggtg ccgaagatga
     4741 atctcgacga ggtgttcgag aagaaggacg aggtgggcac ctcggtgaag accgagctcg
     4801 acgcatcgat gaagatctac ggcttcgaga ttccgaacgc cctggtcacc gatgtcaacc
     4861 cggccgacaa cgtcaaggcg gcgatgaacg agattcagac gcagcagaga ttgcaggtgg
     4921 cggccgcggc gaaaggcgaa gcgaacaaga tcctcattgt caaaagcgcg gaagccgaag
     4981 ccgagtcgaa gcgccttcag ggcgagggca tcgccaagca gcggcgcgcc atcgtcgacg
     5041 gcctgcgcga atcgatcgca gcctttaccg acaagatcgg cgacgtcacc gcgcccgagg
     5101 tgctcaagct cgtgctgatg acgcaatatt tcgacaccat gaaggagatc ggcgtctcgt
     5161 cgggcagcaa ggtgatcctg atgccgcata cgccaggcgg aatgtccgac atcgccgagc
     5221 agcttcgcgc cgcaatcatc tccgcgaacg agacaacgaa ggcataagca gcttctcgaa
     5281 cgtctcgtcg aatgttgcca gacgatcgca accagccctg attgcctcgt gttcgatcac
     5341 gccgtcggcg aaatcgccgc cgcgcgcgag catgcgcaat cccgcccccg cagcaggctg
     5401 gtccgtttcg acgttgcggc tgtggatcac gccggcgagc gtttccgcga tctccgcgcc
     5461 ggaatagcgg taggcacgct tcagcaccca gacggtttcg cacaacacga ccgtcgagac
     5521 cgctatcgca tctgcggatt cgatgtgatc gcgtgccgct cgcgcctgcg tttcgtcgtc
     5581 ccaggtcaga tagcggacga gcacatttgt atcgacggcg agtttcatcg cccgcccgat
     5641 tcaatcgcct tttgcatctg ctcgagagaa accgcccgcc ggacccggcg cctgagcgcg
     5701 ccccgcaact ggcggatgtc ggccgccgcg gcggccggtg cgagagtcac tcgtcctccc
     5761 gggagcagcg acaccctgac cttgccgccc ggcccgattc cgagatgctc gagaacggcg
     5821 cgtttcagcg tcacctggcc cttggccgtt attgtcagct ccgacatgtt gccttaccag
     5881 cctccattgc ttccttacgt agggtaatgg attcgattgc tgccttcaac gggccgccga
     5941 gcctgtctcc ccaaatcccg gccggtgcgg gtccgcgcgc gctcttgccg cgcggttcga
     6001 tttcgccgat cactgggcac aagggcgatt caccgtatcc cgaggggtga caaccgtgcg
     6061 ccatcgtgtg cgcgacaatt ccatgccagg cgcaagctgg cgatcgacaa ccgctgcgca
     6121 tgcgcgagtc gtcgcagtcg catgcgctgc gttctgcgca ggtggccctg cgatggcgca
     6181 gcctgcacag accgaaactc ccccgccgtc ggaccgccca tggatgaatc cgactctcga
     6241 acccgacgcg cgcgccgcac ttctcgaggc gcagatgaca caggacgaga agcttcgcct
     6301 cgtacttggc ttcttcggcg tgaagggcgg cagcatcttc gcgcgaggcg cgccgcccga
     6361 atatcaggcc gggcttcgca atacggcggg tttcattccc ggcattcccc ggctcggtat
     6421 tcccgccctc gtcgaatccg atgccggggt cggcatcgcc aacagcgcca atacgcggcc
     6481 gggagacgaa gcgaccgccc tgccctccgg gctcgcgatg gccgcgacct ggaatcccga
     6541 tcttgtcttt cagggcggag cggcgctcgg ggcggaggcg cgcgaccgcg gctacagcgt
     6601 cgtgctcgcg ggcgcgatga atctcgcccg cgatccgcga ggcggacgca ccttcgaata
     6661 tgcgggagag gatcctctgc ttgcgggaac ggtcgcgggc gcggaagtcg cgggcgtgca
     6721 gagcgcgcat gtggtctcga ccgtcaagca ttttgcgttc aacgaccagg agacgggtcg
     6781 gcgcgaactg agcgcggtga tcggcgaggc ggaggcgcgc gaatccgacc tgctcgcctt
     6841 cgagatcgcc atcgagcgcg gttcgccggg cgcggtgatg tgcgcctaca accggttgaa
     6901 cggcgtctat tcctgcgaaa atccgttcct cctgaccgac gttttgaagc gcgactggaa
     6961 gtttcccggc tgggtgctct ccgactgggg cgcggtgcac agtacggtgg aatccgcgaa
     7021 cgccgggctc gaccaggaat cggcttcggg attcgaccgc cagctctatt tcgcggcacc
     7081 gtttgcgcag gccttggctg acggcagcgt cccgcccgcg cggctcgacg acatggtcca
     7141 tcgtatcctg cgcacgatgt tcgcgcgcgg gataatcgac aatccgcccg ccccgcgcct
     7201 ttccgatatc gccggccatc tcgcgatcgc gcagctggaa gcggaagacg gaatcgttct
     7261 gctccggaac gagggagcgc tgctgccgct caaatcccat ccgcgcagca tcgccgttat
     7321 cggcggacat gccgacatcg gcgtgctgtc cggcggaggc tcgtcgcagg tcatgcccat
     7381 cggccacagc ctggcgaacg aatatccagt cggtggcccg gtgatcgtgc tgcccggcgg
     7441 cgcgcgcgcg atgccggtga gcggctatgt gttcgaccca tattctccgc ttgccgccat
     7501 cgaaaccgcc gcaccggagg cgcgcatcac ctatgccaat ggcgaggaca tcgcggttgc
     7561 cgtcgccgcc gcgaagaacg ccgatgtcgc gatcgttttc gcgctgcaat ggatgacgga
     7621 gggcagcgac gtgccgaacc tttcgctgcc gggcacccag aacgcgctca ttgcagccgt
     7681 cgccgccgcc aacccgcgca cgatcgtggt gctggaaacg ggcggtccgg tgctgatgcc
     7741 gtggctcgcg aacgtgcctg ccgtcgtcga agcgtggtac gcgggaaacg gcggcggcga
     7801 tgcgatcgcc aatgtgctgt tcggcgaagt gaatccctcg gggcggctgc ccgtcacctt
     7861 tcccgcgagc gaggaccagt tgccgcgacc tgtcctgccc ggacccgaca cgcctggcgc
     7921 ctggttcgat gtcgactatg tcgagggcgc cgacgtcggc tatcgctggt tcgagaaaaa
     7981 gaagctcgtg ccgctctttc ctttcggctt cggcct
//
LOCUS       DASZMC010000071         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4167327,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000071 DASZMC010000000
VERSION     DASZMC010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4167327"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VG274_01375"
     CDS             complement(<1..1395)
                     /locus_tag="VG274_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05326.1"
                     /translation="MAALIAAILRAQLLSMWPRSRARRGGMAFSLITGALFYGFWCFI
                     AWGAALFFSSIDEAPYFVPALTTGLAVAMLYWQLMPLLSASFGASLDPRKLLAYPIPH
                     NKLFLIELMLRVTTCAEMTLVIGGVAVGLLRNPLYGWRAAPFILAGALLFAITNLLLS
                     AGTKHAIERWFARARLKEVLMLLFVFGSLLPKLLAYSHVHMSALLRFAPSQVVWPWAA
                     AGRLMVRDSTGLSILTALLWLGVTALFTRQQFERSLVFDADSVRRPERADRSDGLIDR
                     LYRTPSRFLPDPIAVVMEKELRTFARIPRCRLVYAMSCFFGAVFFLPSLRGNRPHSSF
                     FQENALPFVALYGLLMLGGVTFWNSFGFDRSATQGYFCWPVRFRDILIAKNLAVVALL
                     IPQILLVSAMGSLMHLTANPIKILETFGAMTVASLYWLALGNIFSVRMPRPLNPEKMN
                     QMSNKLQALSIWTAP"
BASE COUNT          365 a          501 c          444 g          215 t
ORIGIN      
        1 cggcgcggtc cagatgctca gcgcctgcaa cttattcgac atctgattca tcttctccgg
       61 attcagcgga cgcggcatcc gcaccgaaaa gatgttgccc agcgccagcc agtaaagcga
      121 ggcgacggtc atggcgccga aagtctccag gatcttgatc gggttcgcgg tcagatgcat
      181 gaggctgccc atcgcggaaa ccagcaggat ctgcggaatc agcaacgcaa ccaccgccag
      241 attctttgcg atcaggatgt cgcggaaccg caccggccag cagaaataac cctgcgtggc
      301 cgagcgatcg aagccgaacg aattccagaa cgtgacgcct cccagcatca gcaatccgta
      361 cagcgcgacg aacggcagcg cgttctcctg aaaaaacgaa ctgtgcggac ggttgcccct
      421 caggctgggc aggaaaaaca ccgcgccgaa gaaacacgac atggcgtaaa ccaggcggca
      481 gcgcggaatg cgcgcgaatg tgcgcagttc cttctccatc acaaccgcga tcggatccgg
      541 cagaaagcgc gaaggcgtgc gatagaggcg gtcgatcaaa ccgtcgctgc ggtcggcgcg
      601 ctcgggtcgc cggacggaat cggcatcgaa gacaaggctg cgctcgaact gctgccgcgt
      661 gaacaacgcc gtcacaccca gccacagcag cgccgtcaaa atgctgaggc ctgttgaatc
      721 gcgcaccatg aggcgccccg ccgccgccca cggccacacc acttgcgagg gagcaaagcg
      781 cagcagcgcg ctcatgtgga catgggagta ggcaaggagc ttcggcagaa gtgagccgaa
      841 cacgaacagc agcatcagaa cttccttcag gcgcgcccgc gcgaaccatc gctcgatggc
      901 gtgcttcgtt ccggccgaga gcagaaggtt cgtgatcgcg aataacaggg cgcccgccag
      961 aatgaacgga gccgcgcgcc atccatagag cggattccgc aacagaccca ccgctacgcc
     1021 gccgatgacc agtgtcattt ccgcgcaggt ggtcacgcgg agcatcagct caataagaaa
     1081 cagcttgttg tgcggaatgg gatacgccag cagtttacgc ggatcgagcg acgccccgaa
     1141 gcttgccgag agcagcggca taagctgcca gtaaagcatc gcgaccgcga ggcctgtagt
     1201 gagcgccggc acaaagtacg gcgcctcgtc gatcgacgaa aaaaacagcg ccgcgcccca
     1261 ggcgatgaag caccagaacc cataaaacag cgcgccggta atgagcgaaa acgccatccc
     1321 gccgcgccgc gcgcgcgacc gcggccacat ggaaagcaac tgcgcccgca atatcgctgc
     1381 aatcaatgca gccactccag acgctcatac tcgcgatccg cccccaccgt cttcacgaaa
     1441 acctcttcga gcgtatgaga gccggcgcgc agctcatcca tgctgccctc cgccacaatc
     1501 cgcccctctt ttacgatcgc ggcgt
//
LOCUS       DASZMC010000072         6822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3058728,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000072 DASZMC010000000
VERSION     DASZMC010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3058728"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..954
                     /locus_tag="VG274_01380"
     CDS             304..954
                     /locus_tag="VG274_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE05327.1"
                     /translation="MSRIGRRKNPALKVTREKTAENRAKLVQAAARLFRERGIDGVGV
                     AEICEAAGLTHGALYAQFPSKEALAAEAFDEAFARGYPRMKTAGGRLAPSLRDYLEFL
                     ISPQQRDNLGGCPLTASASEVARKDETLSASFSRGFEQMVAVVESAIDRKVPVAERRD
                     RALTIIAAEIGVIAVARAAAKSRPDLSNRVVAAARRVLTQIGDAPQRPKRTRRKRR"
     gene            complement(1386..2096)
                     /gene="radC"
                     /locus_tag="VG274_01385"
     CDS             complement(1386..2096)
                     /gene="radC"
                     /locus_tag="VG274_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWE05328.1"
                     /translation="MKRDQVFECALPAPDAPHYQGHRERLRGRFLDSGAAALSDYELL
                     ELVLFAAIPRRDVKPLAKTLLAKFGSFAEVVAASRERLSEHLGDGAIAQIKIIEAAAL
                     RLSRTMLMGKPALSSWNALLDYCAAAMARAPKEEFRVLFLDRKNFLIGDEVMNKGTVD
                     HAPVYPREIVKRALELSASALILVHNHPSGDPTPSRADIEMTREVVEAARALRIAVHD
                     HLVIGRGGTASFKALGLL"
     gene            complement(2473..3312)
                     /gene="map"
                     /locus_tag="VG274_01390"
     CDS             complement(2473..3312)
                     /gene="map"
                     /locus_tag="VG274_01390"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWE05329.1"
                     /translation="MLSSQLPVSETIDATRRSNNVVTLHGPDAFEGMRRAGRLAAEVL
                     DLLVPEVAPGVTTARLDALAYQYVVDHGGIPACLGYRGYRHTLCTSLNHVVCHGIPGD
                     KPLRDGDIVNIDVTVIVDSWHGDTSRMYLVGDVKRKALRLVDITYEAMMRGIAAVKPG
                     ATTGDIGHAIQSYAEGAEHCAVVRDFCGHGVGRVFHALPNIVHYGRPGYGLGLKPGMF
                     FTVEPMINLGGFGVKVLNDGWTAVTRDRSLSAQFEHSVGVTEDGVEIFTRSPRGWDRP
                     PYK"
     gene            3433..4188
                     /locus_tag="VG274_01395"
     CDS             3433..4188
                     /locus_tag="VG274_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWE05330.1"
                     /translation="MPNDASPTAAVLLIGDEILSGRTADVNLATIAKFLAPYGIDVCE
                     ARVVRDVAEEIVESLNALRTRFTYVFTTGGIGPTHDDITADAIGRAFGIAVNYHPEAL
                     ALLEPRYKPGEFNEMRKRMARIPAGATLIRNSVSTAPGFQIGNVFVMAGVPMIMRAML
                     EEVGPRLARGATVVTRTISASVGEGKIAAALARIQRDHAGVAIGSYPFYAEAGSGVQL
                     VVRGRDGEAVEVAAKAIESALDREGIKSARIGP"
     gene            4369..4644
                     /locus_tag="VG274_01400"
     CDS             4369..4644
                     /locus_tag="VG274_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05331.1"
                     /translation="MTMRRVLGAMAGGFIFAAAGVAAAGNFTSSAHTDFFSPGSHQFY
                     VWCAGSPDYTTTASGNSAEDAQIRLYDAIKATGRSTCWPVWQGRVRA"
     gene            complement(4721..5608)
                     /locus_tag="VG274_01405"
     CDS             complement(4721..5608)
                     /locus_tag="VG274_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05332.1"
                     /translation="MSEDLTWAVIALFVAITLAAVSFVVWPVWRAPGARALPRALLAG
                     AFATSVLGIGGGLYLYLGSPVLAVRAVASPDTKDVPGLIAALSQRMRERPRDLTGWTL
                     LGRGYLSLNDPQQAAVAFRTASDLAPPQAKPQLLSAYGEALTLAAGTVTPEAEAAFRA
                     ALAGQPKDFAARFYLGEAYAARRDANHALALWQGLLADSPANAPWRAELVDRMAALRA
                     GMGETPDIGQMVEGLAARLRAAPGDFSGWQRLVRAYAVLGQQAKARSALAAAHAAMKG
                     RTHDLAALDAEARSLKIVE"
     gene            5872..6780
                     /gene="nudC"
                     /locus_tag="VG274_01410"
     CDS             5872..6780
                     /gene="nudC"
                     /locus_tag="VG274_01410"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HWE05333.1"
                     /translation="MMPFTGNRLDRASDRRVDAAWIAAKRADPSTFIHPLWKLQPFVT
                     GSDEGPLDAVYFRPGRCETLAAQDAPCVFLGLDGERAMFALDISAAPDPERNGPLADL
                     GRFRDLRSAASLLPLNDVAILGQAKALIDWHQRHGFCPRCGAATMLMDGGYRRFCASC
                     TAEHFPRTDPVVIMLALDGERCLVGRGRQFPPGMFSALAGFVEPGETIEEAVRRELFE
                     ETGIRTGAVTYHATQPWPFPSSLMIGCFAEALSRDIRVDESELAEARWLDRADVRALL
                     AGGHVEGVRIPPPIAIAHHLLKAWAA"
BASE COUNT         1218 a         2233 c         2228 g         1143 t
ORIGIN      
        1 taagtcaact aacttgatat ggtgaggata ggcaaacctg tcaagcatcc ggcctgcttc
       61 cggcgtctcc ggttcgcgcg ggctgccaac gtcgttcaga atgcagatca gtgcgggctt
      121 gggcacctga agcgcgagca cctacgcggc cacgccgtgc tcatccagcc agccatggac
      181 gatcggccgg ccactctgcg tttgccaatc gcccgattgg ccgtccgcgc cccaatcgcc
      241 cggatgaggt tcgcctccgc ggcgcccgtg ccgacttgac ttcaaacatt gtgactacaa
      301 tctttgtctc gcatcgggcg aagaaagaac cccgcactga aggtcaccag ggagaagacg
      361 gcggagaacc gggcgaagct cgtgcaggcc gcagcacgcc tgttccgcga gcgcggcatc
      421 gacggcgttg gagtggccga gatctgcgag gcggcgggac tgacgcacgg cgccctctat
      481 gcgcaatttc cgtcgaagga agcgcttgcc gccgaggcgt tcgacgaagc cttcgcgcgc
      541 ggatacccca ggatgaaaac ggcgggtgga aggctcgcgc cttcgctccg ggactacctg
      601 gaattcctga tttcgcccca gcagagggac aatctgggcg gctgtcccct gaccgcgtca
      661 gccagcgaag tcgcgcgcaa ggacgagact ctcagcgcga gcttttccag gggcttcgag
      721 cagatggtgg ccgtcgtcga atccgccatc gaccgaaaag tgccggtagc cgagcggcgc
      781 gaccgcgcgc tgacgatcat cgccgccgag atcggcgtca tcgccgtcgc tcgcgcggcc
      841 gctaaatcgc ggccggacct gtcgaaccgc gttgtggcgg cagcgcgccg tgttctgacg
      901 caaatcggag atgcgccgca gcgtcccaag cgtacccgtc gaaagaggag atgacgcgcc
      961 gtcttcgcgt tgtcgaagct cacgaggagg cggaagaagc acacggcttc cggacacaaa
     1021 gcgagaaaga tgcaataatc ctcggtcacg accggtcgac ccagcctgga gacgctcagc
     1081 cggcatgtca gcggcacatc gcaagaagac tcatcatctg caaggtcttc atgaacgaac
     1141 gcgagcgacg aacctggaga cgcggcggag cattgtttcc cgtgaaacat tcgtcagcgc
     1201 tttgtttcgc cgtgcggccc tgttttgcag gcgcaacgcc tcaaacgcac ctgttcccac
     1261 cggaaaatca atttgcgcaa gtgcctcgga ttgttcggcg aatcgggaat gttggcattt
     1321 tgatcgcccg tacctaaaag aatcgcctgt atttcgcctg ataaacagga gcgacggcgc
     1381 gccgcttaca gcagccccag cgccttgaag ctcgcggtgc cgccgcggcc gatgacgagg
     1441 tggtcgtgga cggcgatgcg cagcgcgcgg gccgcctcga ccacttcgcg ggtcatctcg
     1501 atgtcggcgc gggagggcgt cggatcgccg cttggatggt tgtggacgag gatgagcgcg
     1561 gaggccgaga gctcgagcgc gcgcttgacg atctcgcgcg gatagacggg cgcgtgatct
     1621 acggtgccct tgttcatcac ttcgtcgccg atgagaaaat tcttgcggtc gaggaagagg
     1681 acgcggaact cctccttcgg cgcgcgcgcc atcgccgccg cgcaatagtc gagcagggcg
     1741 ttccatgagc tgagcgccgg ctttcccatc agcatcgtgc gcgagagccg gagcgccgcg
     1801 gcttcgatga tcttgatctg ggcgatcgca ccgtcgccga gatgctccga cagccgctcg
     1861 cgggacgccg cgacgacttc ggcaaagctg ccgaacttcg cgagcagagt cttcgccagc
     1921 ggcttcacat cgcggcgcgg gattgcggca aacagcacca gctcgagaag ctcgtagtcg
     1981 gaaagggccg cggcgccgga atcgaggaag cgcccgcgca ggcgctcgcg atggccttga
     2041 taatgcggcg catcgggcgc cggcaacgcg cattcgaaaa cctggtcgcg cttcatcggc
     2101 tgctctgtcg cgtcgatgag cgcagcctaa cctcgcagcg ggtcttcggc ggcacgaaat
     2161 tttgtatgaa cttgtcgcgg ggcggcggct cacgaagctt cgcggcagat ccaccgcggc
     2221 gcatgggcgc caagataggc cgaaggcccc tcacccgcgc cagagcagaa atcattgcat
     2281 gtacttgcat ccctctccca cgaggggaga ggggaatcgg agtcttgcac cagcaggaga
     2341 aaatcagcgc gggagattcc ctctcccctt gtgggagagg ggaaccagtc gaatcttcgc
     2401 ttccgctccg aagtgggtga ggggtcgctc ggctcgagtt atctcagcgc cgatgcgcct
     2461 cggcggggcg actcacttgt aaggcggtct gtcccagccg cgcggcgacc tggtgaagat
     2521 ctccaccccg tcctccgtca cgcccacgct gtgctcgaat tgcgccgaca gcgaacggtc
     2581 gcgcgtcacc gccgtccagc cgtcgttcag caccttcacg ccgaagccgc cgagattgat
     2641 catcggctcg acggtgaaga acatgccggg tttcagaccg aggccgtaac ccggccggcc
     2701 gtaatgcacg atattgggca gcgcgtgaaa gacgcggcca acgccgtgtc cgcagaagtc
     2761 gcgcaccacg gcgcaatgct ccgcgccttc ggcgtaggac tggatcgcat ggccgatatc
     2821 gccggtggtg gcgccgggct tcaccgccgc gataccgcgc atcatcgctt cataggtgat
     2881 gtcgacgagg cgcagcgctt tgcgcttcac gtcgcccacc agatacatgc ggctggtgtc
     2941 gccgtgccag ctgtcgacga tcaccgtcac gtcgatattg acgatgtcgc cgtcgcgcag
     3001 cggcttgtcg ccggggatgc cgtggcagac gacgtgattg agggaggtgc agagcgtgtg
     3061 gcgatagccg cgatagccga ggcaggccgg gatgcccccg tggtcgacca catattgata
     3121 ggcgagggcg tcgagccgcg ccgtcgtcac gcccggcgcc acttccggca ccagcagatc
     3181 cagcacctcg gccgccagcc ggccggcacg gcgcatgccc tcgaacgcgt ccggcccgtg
     3241 gagcgtcacg acattgttcg accggcgcgt tgcgtcgatg gtttcggaga cgggcaactg
     3301 cgagctcaac atcggtccaa tctaggcatt ttcgccggcc atccaagccc gcttgccggg
     3361 ggtgcggtcc tcgtgctttc tagcccggaa cctctcaatc ctttgttgcc gacgcgagcg
     3421 cgccagagcc gcatgccgaa cgacgcttcc cccaccgcag ccgtccttct catcggcgat
     3481 gagatccttt ccggccggac cgcggacgtg aacctcgcca ccatcgcgaa attcctcgcg
     3541 ccgtacggga tcgatgtctg tgaggcgcgc gtggtgcgcg atgtggcgga ggagatcgtg
     3601 gaatccctga atgcgctgcg gacgcgcttc acctatgtgt tcacgaccgg cggcatcggc
     3661 ccgacccatg acgacatcac cgccgatgcc attgggcgcg ccttcggcat tgccgtgaat
     3721 tatcaccccg aggcattggc gctcctcgag ccgcgctaca agcccggcga gttcaacgag
     3781 atgcgcaagc gcatggcgcg catccccgcg ggggcaaccc tgatccgcaa cagcgtctcg
     3841 accgcgccag gcttccagat cggcaacgtt ttcgtgatgg caggggtgcc gatgatcatg
     3901 cgcgcgatgc tcgaggaggt gggaccgcgg ctggcgcgcg gcgcgacggt ggtcacgcga
     3961 acgatttcag cgtcggtcgg cgaggggaag atcgcggcgg ccctcgcccg gatccagcgc
     4021 gaccatgccg gggtcgcgat cggctcctac cccttctacg ccgaggctgg ctccggcgta
     4081 caattggtgg tgcgcggacg ggacggcgag gcggtggaag tggcggcgaa agccatagaa
     4141 tccgcgctcg accgtgaagg aatcaaatcg gctcgaattg gcccttgaca cgcaactatc
     4201 tgctaaggct ctgacgaaca agcaaaaaaa tgcgtgacag cgcccggccg ctcgtatagc
     4261 ttccttggca gagtgggtat acgccacccg gccgaatttg ggggaaacag cctaagcgta
     4321 aacagtgcgg gaacccgtcg gttcccctcc aaacgcagga gtaaaaagat gacgatgcgg
     4381 cgggttctgg gggcaatggc gggcggcttt attttcgcgg cggcaggcgt ggcggctgcg
     4441 ggaaacttca cctccagcgc acatacggac ttcttctcac ccggcagcca tcagttttat
     4501 gtctggtgcg ccggctctcc cgactacacg accaccgcat cgggcaacag cgccgaagac
     4561 gcccagatcc gtctctatga cgcgatcaag gcgaccggcc gctccacctg ctggccggtc
     4621 tggcagggcc gcgtccgggc ctgattaccg acccgggttc catccttcga caagctcagg
     4681 atgaggacga tttcctcgcc ctgaacctgt cgaagggcga ctactccaca atcttgagcg
     4741 acctggcctc ggcatcgagg gcagcaagat cgtgggtgcg ccccttcatc gcggcgtgcg
     4801 ctgcggccag cgcggaccgc gccttggcct gctggccgag aaccgcatag gcgcgtacca
     4861 gcctctgcca ccccgagaaa tcgccgggcg ctgcgcgcag acgtgcggcg agcccttcca
     4921 ccatctgccc gatatcgggc gtttcgccca ttccggcccg cagcgcggcc atgcggtcga
     4981 ccagctcggc ccgccagggg gcgttcgcgg gtgaatccgc gagcaggcct tgccacagcg
     5041 ccagcgcatg gttggcatcg cgccgcgccg cataggcctc gcccagatag aagcgggcgg
     5101 cgaaatcctt cggctggccg gcgagggcgg cgcggaaggc cgcttccgct tccggcgtta
     5161 ccgtgcccgc ggcgagcgtc agcgcttcgc cataggccga aagcaattgc ggtttcgcct
     5221 gcggcggcgc caggtcgctg gcggtgcgaa aggcgacggc cgcctgctgt gggtcgttca
     5281 gcgagagata gccgcggccg agcagggtcc atcccgtcaa atcgcgcgga cgctcgcgca
     5341 tgcgctgcga aagcgcggcg ataagcccgg gcacatcctt cgtatcggga gatgcaacag
     5401 cgcgaaccgc cagcaccggc gaacccaggt agagatagag ccctccgcca ataccgagga
     5461 ccgatgtcgc gaaggcgccg gcgaggagcg cgcgcggaag cgcgcgggca cccggcgcac
     5521 gccagaccgg ccacaccacg aaactgacgg ctgcgagcgt gatcgcaaca aacaaggcaa
     5581 tcaccgccca ggtcaaatct tcagacacgc ctgaaatcat gcggcgttgc tacactgccc
     5641 gcgtcccgga cgcgaccctc gtccgggatc acccttcgac ctgtctgcgc gaagctgaag
     5701 cttcgcttcg gcgaaggcag gcaagctcag ggtgaggatt tccctcatcg tcctgagccc
     5761 gtcgaaggac gacaagccca cccttcgctt gtcgaaccat gaggcacctt cccttcgaca
     5821 agctcggggt gaggacgtat taggcggacg cgccccacgg agatttcgcc gatgatgccg
     5881 ttcaccggaa acaggctcga tcgggcaagc gaccgccgcg tggacgcggc ctggatcgct
     5941 gcgaagcgcg ccgatccatc caccttcatt catccgttgt ggaagctgca gcctttcgtg
     6001 acggggagtg acgagggtcc actcgatgcg gtgtatttcc ggcccgggcg ctgcgaaacc
     6061 ctggcggcgc aagatgcgcc ttgcgtgttc ctggggctcg atggggaacg ggccatgttc
     6121 gcgcttgaca tttccgctgc gcccgacccc gagcgcaacg gaccgctcgc ggatctgggc
     6181 cgatttcgcg acttgcgttc cgccgcatcg ttgttgcccc tgaacgacgt cgcgatcctt
     6241 ggtcaggcca aggcgctgat cgactggcac cagcgtcacg gcttctgccc gcgctgcgga
     6301 gctgcgacaa tgctgatgga tggcggctac cggcgcttct gcgcgagctg caccgcggag
     6361 cattttccgc gcaccgatcc cgtggtcatc atgctggccc ttgatggcga gcgttgtctc
     6421 gtggggcgcg ggcgccagtt tccgccgggc atgttctcgg cccttgccgg tttcgtcgag
     6481 cccggtgaga cgatcgagga agcggtgcgc cgcgaattgt tcgaagaaac gggaatccgc
     6541 accggcgccg tcacctatca cgcaacccag ccctggccgt ttccgtcctc gctgatgatc
     6601 ggctgcttcg ccgaagcgct gagccgcgac atccgtgtcg acgagagcga gcttgccgaa
     6661 gcgcgctggc tcgatcgcgc cgacgtgcgc gcgctgctgg cgggcggcca tgtcgaaggc
     6721 gtgcgcatcc cgccgccgat cgcgatcgcc catcatctgc tcaaggcgtg ggcggcatag
     6781 atgacagcgc tccgcgcaag tctcgaagcg atcgaggccg ac
//
LOCUS       DASZMC010000073         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1128969,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000073 DASZMC010000000
VERSION     DASZMC010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128969"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VG274_01415"
     CDS             complement(<1..1105)
                     /locus_tag="VG274_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE05334.1"
                     /translation="MTTPIRDPQISGGSAIIAARLDRLPVTRSVWIRVILLSLGGFFE
                     FYDMFFTGYIGPGLVRSHILTPTSGAFFGSTGLASFVAAMFSGLFLGTSIFSFLADRL
                     GRRAIFTYSLLWYSVATVIMAFQNDAFGLNLWRFIVGIGVGVELVTIDAYLSEIVPAT
                     LRGRAFACNNVIQFLSVTVVAFLSWLLVPRAPFGLDGWRWVMLIAAVGAIVVWWIRLR
                     VPESPRWLARHGRIAEAERIMDQMEAEALADGGTLAPLAALPEPESTKGSFAEIWKGP
                     YLRRTIMLSIFNLAQAIGYYGFANWVPTMLVKQGITLTNTLLYTFLIAIAAPFGPLLA
                     GIFADKLERKWQIVGCAAMVAISGTLFGQTTFVP"
     gene            complement(1192..1866)
                     /locus_tag="VG274_01420"
     CDS             complement(1192..1866)
                     /locus_tag="VG274_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWE05335.1"
                     /translation="MTDATIVTTVPPDLIDVPKCLRDPSQRTARLALLHWPLMDPLTA
                     RVDSTRAEADERDVPYFDPLDGGTVAECLFLLEAPGPQARKSGFVSRNNPDETAKNWF
                     ELNVAAGIDRRRTILWNIVPWYIGSEGRIRPATTTDIQRGLPYLLRLIALLPALRVIV
                     LVGKKASKARFYLQSVDCRATILEMPHPSPSFINRSKGNRDLLLHNLQRVRDTLDETD
                     YLAAST"
     gene            complement(1899..>3470)
                     /locus_tag="VG274_01425"
     CDS             complement(1899..>3470)
                     /locus_tag="VG274_01425"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HWE05336.1"
                     /translation="EIGVPGKTNPMRTAKDVGDPWLLQEGFTLAWVGWEFDMKPDPDL
                     IKLLNVPVLRGITGPVRAQVQVDAKTTTSSLADRGLAAYVVADMKGGSMTVRDGFDGA
                     RQEIARGDWHFTADGMSVEYPKGFEPGRIYEVVYTGKDPVVAGVGMAAFRDYVSYAKK
                     QGEVKYAIAYGPSQDGRFLRNFVRDGFNGDEQGKRAFDGVWAHIGGSGIGSFNVRFAQ
                     PSRTRWDYPTDLPPYTPDGLLAKSRAAGVAPKLFLTNNSNEYWNSGGSLVHTTTDGKH
                     EIPPPADARIYYVAGTQHESASAAPTAAVQYPWNNMQVRWFMNAMVIAMNDWITKGTN
                     PPPSRYPSVERGELTDLAGLKFPKLTGVHPPARVFRPRTLDFGPDFLTKGIVSIEPPK
                     AGALLAALVPQVDDDGNEIAGVHLTQLSVPLGTYTGWNLRQTKIGASDATYVNIGSTF
                     PFALTKADREAAHDTRRSIEERYKSEADFLAKTEAAAHELTKQRFVLDRDIPRIVELA
                     KTHWRELHAQVSAKN"
BASE COUNT          709 a         1039 c         1093 g          629 t
ORIGIN      
        1 cggggacgaa cgtggtctgg ccgaagagcg tgccggaaat cgcgaccatg gccgcgcaac
       61 ccacgatctg ccatttgcgc tcgagcttgt ctgcgaaaat cccggccagc agcggaccga
      121 acggcgcggc aatggcgatg aggaacgtgt agagcagcgt gttggtgagc gtgatgccct
      181 gcttcacgag catggtgggc acccagttcg cgaagccata atagccgatg gcctgcgcca
      241 gattgaagat cgacaacatg atggtgcgcc gcaggtaggg acccttccag atttccgcga
      301 aacttccttt ggtggattcg ggctcaggca aggcggcgag cggagccagc gtgcccccat
      361 ctgcaagagc ctcagcttcc atctggtcca tgatgcgctc ggcttcggcg atgcgcccgt
      421 gccgcgccag ccagcggggg ctttccggca cgcggaggcg aatccaccag acaacgatcg
      481 cgccgactgc ggcgatcagc atcacccagc gccagccgtc gagtccgaag ggcgcgcgcg
      541 ggaccagcag ccacgagaga aacgcgacga cggtgacgga cagaaactga atcacgttgt
      601 tgcacgcgaa cgctcggccg cgcaaagtcg cgggcacgat ttcggagaga tacgcgtcga
      661 tggtgacaag ctcgacaccg acgccgatgc cgacgatgaa tcgccacagg ttcaatccaa
      721 aggcgtcatt ctgaaacgcc atgatgaccg tggccaccga ataccacaac agtgaataag
      781 taaagatggc gcggcggccc aggcgatcgg ccagaaagct gaagatcgaa gtgccgagaa
      841 aaagcccgga gaacatggcg gcgacgaagc tcgcaaggcc agtggagccg aagaatgcgc
      901 cggaggtggg cgtgaggatg tggctgcgga caagaccagg accgatgtag cccgtgaaga
      961 acatgtcgta gaactcgaaa aagccgccga gcgaaaggag gatgacgcgg atccacaccg
     1021 agcgcgtgac gggcagccgg tccaggcgcg cggcgatgat ggcggagccg ccactgattt
     1081 gcgggtcgcg aatgggcgtg gtcatcggcg tttaaacata ccacgggctt cgctcctatt
     1141 acgaaccgcg ctgccttagc gacagccaaa tcgaataggg ccgcaccgga atcacgtgga
     1201 ggcggccaga tagtcggtct cgtcgagcgt atcgcgaacg cgctggaggt tatggaggag
     1261 caggtcgcgg ttgcccttac tgcggttaat gaagctgggg cttgggtgcg gcatctccaa
     1321 gattgtcgct cggcagtcca cactctgaag atagaatcgg gctttacttg ccttcttacc
     1381 gaccagaacg ataacccgta acgcagggag aagcgcgatc aaccgtagca agtacggcaa
     1441 tccgcgctga atgtcagttg tggtcgcagg ccgaatccgt ccttcgctcc cgatgtacca
     1501 cgggacaata ttccacaaga tcgttcgacg gcgatcaatg ccggccgcaa cattcaactc
     1561 gaaccagttt tttgcagtct cgtcaggatt gttgcgggaa acgaacccgg acttgcgcgc
     1621 ctgaggccct ggcgcctcga gtaaaaacaa acactccgca acggtcccgc catccagcgg
     1681 atcgaagtat ggcacgtctc tctcgtccgc ttccgcccgt gtcgaatcaa cgcgcgcagt
     1741 aagcggatcc atcaagggcc aatgcagcaa cgccaagcga gcagtacgct gcgacggatc
     1801 acgcaggcac ttcggcacgt cgatcaaatc aggtggaacg gtcgtcacaa ttgttgcgtc
     1861 agtcatcggg ttcaccctcg gctttatcac tgaatcgact aatttttcgc cgatacctgc
     1921 gcgtgcagct cgcgccagtg ggtctttgcg agctcaacga tgcgcgggat gtcgcggtcg
     1981 agcacgaagc gctgctttgt tagctcgtgc gcggcggctt cggtcttcgc gaggaaatcg
     2041 gcttcgcttt tatagcgctc ctcgatggag cggcgcgtat cgtgcgcggc ttcacgatcg
     2101 gccttggtca gcgcgaaggg gaacgtggag ccgatgttta catacgtcgc gtctgaggcg
     2161 ccgatctttg tttgacgcag gttccatcct gtataagtgc cgagtggcac gctgagctgc
     2221 gtaagatgta cgcccgcgat ttcgttgccg tcgtcgtcca cttgcggaac aagggccgcc
     2281 agcagcgcgc ctgccttggg cggctcgatc gatacaatgc ccttcgtcag gaagtcgggg
     2341 ccgaaatcga gcgtgcgcgg ccgaaagacg cgtgcggggg gatgaacgcc cgtgagcttg
     2401 ggaaatttca agcccgcgag atccgtgagc tcgccgcgct ccacgctcgg gtagcgcgag
     2461 ggaggagggt ttgtgccttt ggtgatccaa tcgttcatcg cgatcaccat cgcgttcatg
     2521 aaccagcgca cctgcatgtt gttccacgga tactgcacgg cagccgtcgg cgcggcggag
     2581 gcgctttcgt gctgcgtgcc cgcaacataa taaatacgcg cgtcggccgg aggcggaatc
     2641 tcatgcttgc cgtccgtggt cgtgtgcacc agcgatccac cggagttcca atactcgttc
     2701 gaattgttgg tcaggaacaa tttcggagcg acgcccgccg cgcgcgactt cgccagcagt
     2761 ccgtcgggcg tgtagggagg cagatcggtg ggataatccc agcgcgtgcg cgaaggctgt
     2821 gcgaagcgca cgttgaagct gccaatgccg ctgccgccga tgtgcgccca cacgccgtcg
     2881 aacgcgcgct tgccctgctc atcgccattg aagccatcgc gtacgaaatt gcggaggaag
     2941 cggccgtcct gcgaggggcc gtaggcgatc gcgtatttca cttcgccctg cttcttcgcg
     3001 taggaaacgt agtcgcggaa agcggccatc ccgacgccgg ccacgacggg atctttaccc
     3061 gtgtacacaa cctcatatat acgcccgggc tcgaagccct tgggatattc gacgctcatg
     3121 ccatcggcgg tgaagtgcca atcgccgcga gcgatctcct ggcgcgcgcc atcgaatcca
     3181 tcgcgcacgg tcatgctgcc gcccttcatg tcggccacca cgtacgccgc gagtccacga
     3241 tcggctagcg acgacgtagt cgtcttcgcg tccacctgca cctgtgcgcg cacgggtccc
     3301 gtgatgccgc gcagcacggg aacgttcagc aatttgatga gatccggatc gggcttcata
     3361 tcgaattccc agccgaccca ggcgagggta aagccctcct gtagcagcca tggatcgccg
     3421 acgtccttcg ccgtgcgcat gggattggtc ttgccgggca cgccgatttc
//
LOCUS       DASZMC010000074         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2791087,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000074 DASZMC010000000
VERSION     DASZMC010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791087"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VG274_01430"
     CDS             <1..898
                     /locus_tag="VG274_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05337.1"
                     /translation="GINKHNVIAGAWIDASDVAHGFIGPLNGAYTSFDFGGASTGTLP
                     RALDDKGNIVGFAEDPNFSIGEEFLRQANGTIVPIQKDGTPLNGVAQGIITKGETSTG
                     DYLDPNTGNRTGYLATDGLYQSDVSLNLDIYSTNPRSLNHFGTLAGFYVESDKVTTHG
                     FILKNGVLQVVDADNSGTTVLEGINKKEVAAGQVSDASGNPHAFVYDNKTGVFTMINV
                     NDGSSEQQAWGINDRGQVAIATGIGASYIYCPRDVQCPDGGHAVADGRSWKAQRGASL
                     QFDRNGRTGVKAAAHATHGTAQ"
     gene            complement(1027..1683)
                     /gene="purN"
                     /locus_tag="VG274_01435"
     CDS             complement(1027..1683)
                     /gene="purN"
                     /locus_tag="VG274_01435"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWE05338.1"
                     /translation="MTPRAKVGVLVSGRGSNLQALIDACAGADFPAEIVCVISNVASV
                     EGLARAERAGIATHVIAHGGFKLREEFDMELDAVLTRAAVSLVCLAGFMRVLSDVFVE
                     KWRGRLINIHPSLLPGFKGLDVHGRVLASGARISGCTVHFVAPELDSGPIIAQAAVPV
                     MPNDTEETLAARTLAAEHRLYPHALKLLAEGRVKLEGERAVFTNISEAEGTLVNPPLD
                     "
     gene            complement(1749..>1939)
                     /locus_tag="VG274_01440"
     CDS             complement(1749..>1939)
                     /locus_tag="VG274_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05339.1"
                     /translation="LSGVGGWAGALANAAIDPRVRNGALGYTGAMGDLPGVTSGGSVL
                     LVFALPNVTNNQNRANAR"
BASE COUNT          389 a          602 c          613 g          335 t
ORIGIN      
        1 cgggatcaac aaacacaatg tgattgccgg cgcctggatc gatgcaagcg acgtcgccca
       61 cggcttcatc ggtccgctca atggcgccta cacgagcttc gattttggcg gcgccagcac
      121 gggaacactg ccgcgcgccc tggacgataa aggcaatatc gtcggcttcg ccgaagatcc
      181 gaacttttcg atcggcgagg aattcctgcg gcaggcaaac ggcacgattg ttccaatcca
      241 gaaagacggt acgccgctca atggcgtcgc ccagggaatc atcacgaaag gcgaaacgtc
      301 gaccggcgac tatctcgatc ccaataccgg caaccgaacc ggatatcttg ccacggacgg
      361 cttgtatcaa tccgatgtca gcctgaacct cgacatatat tcgacgaatc cgcgctcgct
      421 caaccatttc ggaacgctgg ccgggttcta cgtcgagagt gacaaggtca ccacgcacgg
      481 cttcattctg aagaacggcg ttttgcaggt cgtcgacgcc gataacagcg gcacgacggt
      541 gctcgagggc atcaacaaga aggaggtcgc cgcgggtcag gtctctgacg cctccggcaa
      601 tccgcatgcc tttgtctacg acaacaaaac cggtgtgttc acgatgatca acgtgaatga
      661 cggttcttcc gagcagcagg cgtggggcat caacgacagg gggcaggttg cgatcgccac
      721 cggcatcggc gcgtcataca tctactgccc acgtgacgtt cagtgcccgg atggcggtca
      781 cgctgtcgcc gatgggcgtt catggaaagc tcaacgtgga gcttcgctgc agttcgaccg
      841 caatgggcgc acaggcgtaa aagcggccgc gcacgcgacg cacgggacgg ctcagtaacg
      901 tcgttcatcg gaagaccggc tgcgaagcat gcgcgcttcg cagccgacct ttttggaccc
      961 ctcacccaag cagagggatg caggtagggg cagccaattc tgctctgtcg cgggtgaggg
     1021 gtaggctcag tcgagtggcg gattgacgag cgtgccttcg gcttcgctga tgttggtgaa
     1081 cacggcgcgc tcgccttcca gtttcacgcg accctccgca aggagcttca gtgcatgggg
     1141 atagaggcgg tgctccgctg caagcgtgcg ggcggcgaga gtctcctccg tatcattcgg
     1201 catcaccggc accgccgcct gcgcgatgat cggtccggaa tcgagttcgg gtgccacaaa
     1261 atgcaccgtg cagcccgaga tgcgcgcgcc cgaagcgagt acgcggccgt gcacgtcgag
     1321 gcccttgaag ccgggcagca gggaagggtg gatgttgatc agccgcccgc gccatttctc
     1381 gacgaacacg tcggacagca cgcgcatgaa tccggcgagg caaacgagac tcacggcagc
     1441 gcgggtgaga accgcgtcca gttccatgtc gaattcttcg cgcagcttga atccgccatg
     1501 cgcgatgaca tgcgttgcaa tgccggcgcg ttcggcacgc gcgagccctt cgacgctcgc
     1561 gacattggaa atcacgcaca cgatctccgc cggaaaatcc gcacccgcgc aggcgtcgat
     1621 cagcgcctga agattgctcc cccgcccgga gacgagcacg cccaccttcg cgcgaggggt
     1681 catgcttcgc tccgatgggc gcgcgcacgt ccttcaacct ccccacaagg gggaggtgaa
     1741 tgtttcgctt atcgcgcatt ggcacggttc tggttgttgg tgacgttcgg gagtgcgaag
     1801 acgaggagga cgctgccgcc gctggtcacg ccggggagat cgcccatcgc gccggtgtaa
     1861 cctagggcac cgttgcgcac gcgcgggtcg atagcggcat tggcgagcgc gccggcccag
     1921 ccgccgacgc cggagagca
//
LOCUS       DASZMC010000075         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1417913,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000075 DASZMC010000000
VERSION     DASZMC010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417913"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..2107
                     /locus_tag="VG274_01445"
     CDS             365..2107
                     /locus_tag="VG274_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05340.1"
                     /translation="MNNAHPPKPRFALSVGIIGHRPNRLPEEMCPRIGREIAETLAAI
                     RVEAFAAHARHADCFSEFAPKLTLVAALAEGADRMAAEAALELGFELEAPLPFAVDEY
                     EKDFDNQNARASFRGFLDKARAVLELDGDRMQAGKSYATGGLVVLDMSDLLIAVWDGG
                     PPHGRGGTTELIAEAGRRGMPIIRVDATGKNLPRLHWRGLDDRHTTPMHIDDHPAAAV
                     EEALATAIEAQIRPPQNPAERSGLDRFLVEGSRRLTARIEYPLLMFLLCVRWPRAGDI
                     LVPQPARLADELSATGSGASAITTAFGWADAIAVRFAQDFRSAVIANFLVSAVAVLVA
                     ALNFPYPWSAIELVLVTFLVVNALVGYKRRWHHRWIESREVAERLRVAAPMHALATRA
                     PGPFGTAPTWPAWYARAISREAGLCRGRLDKEGLAAARRGLQALLNTQAAYHQTTAWR
                     FRRLHGRLALAGLSLFVAAFLLSAGAFAGIALRLLSVTPDMQYWMIVASASLPAFASA
                     GYGIRMIGDFDGAAKRSARMKAQLDALASSIGKGGAEFEWLRDRAHKASDVMLGDVAI
                     WRLAVESRELEMPG"
     gene            2270..2515
                     /locus_tag="VG274_01450"
     CDS             2270..2515
                     /locus_tag="VG274_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWE05341.1"
                     /translation="MSLNIKNPETHRLAQELAELTGESMATAVTAAVRERLARQRRKG
                     LARKLMEIGRDCSARMSTKTRRTDHGSLLYDERGLPK"
     gene            2512..>2717
                     /locus_tag="VG274_01455"
     CDS             2512..>2717
                     /locus_tag="VG274_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05342.1"
                     /translation="MIVDTSAVLAILKNEKDAATFAEAIEANPECRMSVVNYVEAAVV
                     LDRDPDPVVGRKLDALRERAAITLV"
BASE COUNT          473 a          862 c          900 g          482 t
ORIGIN      
        1 gttcttcatc gaccgccccg gaattctgcg acggagccgg cggcgtcgct cagcatagcg
       61 gcccgcggcc ggctagatag cagctagacc actttttgat ctttagtgtc tcgtgtgtcg
      121 gcagattttt aacgcagagt acgcggagga tgcgcggagg gcgcagggta agaatcgcgc
      181 gcctgcggcg cgccaactct tttccgcgtc cactgcgctg cctccgcgta ctctgcgtta
      241 aaaatctgcc gacccacgag tcactaaaga tcaaaaggtg gtaccagctg ctatctagcc
      301 ggccgcgggc cgcaatgttg aggaaagcgg tacgctccgt cgcagaatac cggggcggtc
      361 aatgatgaac aacgcgcatc ccccaaaacc gcgctttgcg ctatcggtcg ggatcatcgg
      421 gcaccggccg aacaggctgc ccgaggagat gtgtccgcgc atcgggcgcg agattgccga
      481 aacgctcgcg gcgattcgcg tcgaagcgtt tgcggcgcat gcgcgccatg ccgattgctt
      541 ttcggaattc gcgccgaagc tgacgcttgt cgccgcgctt gccgaaggcg ccgaccgcat
      601 ggcggcggaa gccgcgctgg agctgggctt cgagctggag gcgccgctgc ctttcgcggt
      661 cgacgaatac gaaaaggatt tcgacaatca aaacgcgcgc gcgtcgttcc gcggtttcct
      721 cgataaggcg cgcgcggtgc tggagctcga cggcgaccgc atgcaagcgg gcaagtccta
      781 cgcgacgggg ggtctcgtcg tgctcgacat gtcggatctt cttatcgccg tatgggacgg
      841 cggtccgccg catgggcgcg gcggcacaac agagctcatc gccgaagccg gccgtcgcgg
      901 catgcccatc attcgcgtcg acgcgacggg gaaaaacctc ccgcggctgc attggcgcgg
      961 tctcgacgac cgccacacca cgccgatgca catcgacgat catcccgccg cggcggtcga
     1021 ggaagcactt gcaacggcca tcgaagcgca gatccggccg ccgcagaatc ccgccgaacg
     1081 aagcgggctc gatcgcttcc tcgtggaagg aagccggcgg ctcacggcgc gaatcgaata
     1141 tccgctgctc atgttcctcc tttgtgtgcg ttggccgcgc gccggcgaca ttctcgtgcc
     1201 gcagcccgcg cggctggcgg atgaattgag cgccaccgga tccggggcgt cagccattac
     1261 cacggcgttc ggttgggcgg acgccatcgc cgtgcgtttc gcgcaggatt ttcgcagcgc
     1321 cgtgatcgcc aatttcctgg tctccgccgt cgccgttctc gttgcggcgc tgaacttccc
     1381 ctacccctgg agcgcgatcg agctggtgct ggtgacgttc ctcgtcgtca acgccctggt
     1441 cggctacaag cggcgctggc accatcgctg gatcgaatcg cgcgaagtcg ccgaacggct
     1501 gcgcgtcgcg gcgccgatgc atgcgctcgc cacgcgggcg ccgggtccat tcggcacggc
     1561 gccgacatgg cccgcctggt acgcacgcgc catctcgcgg gaggcgggac tgtgcagagg
     1621 ccgtctggac aaggaagggc tcgccgctgc gcggcggggg ttgcaggcgc ttctgaacac
     1681 gcaagccgct taccaccaaa cgacggcgtg gcgattcagg cggctgcatg gccggctggc
     1741 gttggcggga ctgtccttgt tcgtcgcggc gttcctgctc tccgcgggag catttgccgg
     1801 aattgcgctc cggctcctgt ctgtcacgcc agacatgcaa tattggatga tcgtcgcttc
     1861 cgcgagcctg ccggccttcg cctctgcggg ctacggcatc cgcatgatcg gcgatttcga
     1921 tggggcggca aagcggtcgg cgcgcatgaa ggcgcaactg gatgcgctgg cgtcttcaat
     1981 cgggaaaggc ggcgccgaat tcgaatggct gcgcgaccgc gctcacaagg caagcgacgt
     2041 gatgctcggc gacgttgcca tctggcgcct cgcggtcgaa agccgggagc tcgagatgcc
     2101 ggggtgatcg cgggcaaagc gacgctcgtg cttttgctgg gtcagagtgc gaacaacagc
     2161 cccgaccttg cgcttgacgc tgagaaacta ttggttttcc cgatcggctg ttgacgcatc
     2221 tcggcgctct agggtagcat tctggatatt tatggtagaa tgatacccaa tgagccttaa
     2281 catcaagaat cccgagacgc accggcttgc gcaggagctg gcggaactga ccggcgagag
     2341 catggcgacg gcggtgacgg cggccgtacg cgagcgcctc gcgcgccagc gccgcaaagg
     2401 ccttgcccga aagctgatgg aaatcgggcg cgattgctcc gcccggatga gcacgaagac
     2461 cagaaggaca gatcacggca gtctgctcta cgacgaacgc gggctcccga agtgatcgtc
     2521 gacacttcgg cggttctcgc gattctgaag aacgagaagg acgctgccac gttcgccgaa
     2581 gccatcgaag caaatcccga gtgccggatg agtgtggtga actatgtcga agcggcggtt
     2641 gttctcgacc gggaccccga tcccgtcgtc ggccgcaagc tcgacgccct gcgcgagcgc
     2701 gccgcaatca cgctcgt
//
LOCUS       DASZMC010000076         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1417973,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000076 DASZMC010000000
VERSION     DASZMC010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417973"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1372)
                     /locus_tag="VG274_01460"
     CDS             complement(179..1372)
                     /locus_tag="VG274_01460"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWE05343.1"
                     /translation="MIAGEPKTMIVAGVMSGTSADGVDVAICRVSPGAERVPRVRVLA
                     HHAMAYPAKLRAAVLTAMDAKNTSTAELARLNWRLGEVYAEAVESAAREASVQPQLIA
                     CHGQTIYHQGTATKYLGAPVRCTWQTGEAAVIAERLRVPVISDFRAADMAAGGQAAPL
                     VPMFDFCVFRDAKRSRVLLNLGGIANVTVLPAGCRAEDVVAFDTGPANMVIDACMERL
                     FGRGFDRNGEIAARGRVLESVLAKLLRARYFSAPPPKSCGREEYGETFVSQFIAACER
                     KSNRKEDVIATATALTASTIADAYVRFCKPKFEKLAPKAKAELIAAGGGVSNATLMRM
                     LSESLRAHGVRVRSIEETGLNASAKEAAAFALLGWLTWHGLPGNLPSATGAKRPVVLG
                     KVTLA"
     gene            complement(1369..>1554)
                     /locus_tag="VG274_01465"
     CDS             complement(1369..>1554)
                     /locus_tag="VG274_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05344.1"
                     /translation="QQVREAVPVKERLPTLQMTMFTPLSQRIVDRVEALDVDGLTPRE
                     ALQILGELQRELKGGTA"
BASE COUNT          280 a          571 c          425 g          278 t
ORIGIN      
        1 gcgtcgtaaa tcaagccgcc aacgcgctcc gactgcgcgt ccgttccttg tcgcaggtcc
       61 agattgttcg gcgagctctc gatgaggaat cgcagcgtct cagggtcctg ctgccgcccg
      121 ttgcatgcag cgagcggtaa cagcagcgcc agcgctgcgg cgcacaacct ccgaatcctc
      181 acgcgagcgt caccttgccc agaaccactg gccgcttcgc cccggtcgcg cttggcaggt
      241 tgccaggcag accatgccac gtcagccagc cgagaagagc aaacgccgcg gcctcctttg
      301 cgctcgcgtt caaacctgtc tcttcgatcg atcgcacccg cactccgtgc gcccgaagcg
      361 attcactcag cattcgcatc aacgtggcat tgcttactcc gccgcctgca gcaatcaact
      421 ccgccttagc cttcggcgcg agtttctcga acttcggctt gcagaagcgt acatacgcat
      481 cggcgatggt gctcgcggtc aaggccgtcg cagtcgcaat cacatcttcc ttacgattgc
      541 tcttgcgttc acatgccgca ataaactggc tcacgaatgt ctccccgtat tcctcgcgcc
      601 cacaggactt tggcggcggg gcagagaagt accgcgcgcg cagcagcttc gcgaggacac
      661 tctccagcac gcgcccccgc gcagcgatct cgccgttgcg atcaaaccca cggccgaaca
      721 ggcgctccat gcaggcgtcg atcaccatgt tggcgggtcc cgtgtcgaag gccaccacat
      781 cctctgcgcg gcaccccgcc gggagcaccg tgacatttgc gatgccgccg aggttcagca
      841 gaacgcggct gcgtttcgcg tcgcggaaca cgcaaaaatc gaacatcgga accagcggcg
      901 ccgcctgtcc acctgcagcc atatcggcgg cccgaaagtc gctgatcacc ggcacgcgca
      961 ggcgttcggc gataaccgcg gcctcgcccg tctgccacgt gcaccgcacg ggcgcgccaa
     1021 gatatttcgt cgccgtgccc tggtgataga tcgtctgccc atggcacgcg atcaactgcg
     1081 gctgcactga cgcttcgcga gcggcactct caacagcttc cgcgtaaacc tcgcccagcc
     1141 gccagttcag tcgagccagc tccgccgtgc ttgtgttctt cgcatccatc gcggtgagca
     1201 ccgccgcgcg caatttggcc ggatacgcca tcgcatgatg cgccagcacc ctcacgcgcg
     1261 gcacacgttc tgctcccggc gacacgcggc agatcgccac atcgacaccg tccgccgatg
     1321 tgccgctcat cacgcccgcc acgatcatcg tcttcggttc gccagcgatc acgccgtgcc
     1381 gcctttcaac tcgcgctgta attcgccgag aatctgcagc gcctcgcgcg gcgtcaggcc
     1441 atccacatcc agggcctcca cacgatcgac aatcctctgc gagagcggcg taaacattgt
     1501 catctgcagc gtaggcaggc gctccttcac gggcacagct tcacgaacct gctg
//
LOCUS       DASZMC010000077         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_34573,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000077 DASZMC010000000
VERSION     DASZMC010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34573"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..767
                     /locus_tag="VG274_01470"
     CDS             399..767
                     /locus_tag="VG274_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWE05345.1"
                     /translation="MRFMVIVKANEDSEAGALPSKELFAEMGKFNEELVKAGVLLAAD
                     GLQPSSKGARVKFSGQQRTVIDGPFSETKELIAGYWVWQVKSKEEAIEWAKRIPNPTG
                     DESEIEIRQVFEPERSDAPN"
     gene            898..>1502
                     /locus_tag="VG274_01475"
     CDS             898..>1502
                     /locus_tag="VG274_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HWE05346.1"
                     /translation="MTTTVRTPPAHAPPARIPMTSLRKTALVAGIFYLITFISIPTLA
                     LYVPVRNHRDWILSSGGHTAVLVGGLLEVIVALAGIGTAVTLYPVLKRQHEGLALGFV
                     TSRLLEGSMIFTGVVSLLSLVTLTHGPGGAAGPNAAALLATGASHVAVYNWTFLLGQS
                     LMPAINAILLGTLLYRSRLVPRALPVLGLIGAPLLLTADIAT"
BASE COUNT          304 a          515 c          434 g          249 t
ORIGIN      
        1 agcttgctcc acatcacctt gctctgaccg tccggaacca caaagagtgg atgaaccagg
       61 ggctcacgcg atcgccgccc tccggccgat tgctttcgat tcgacagcag tctacctcgc
      121 tgccactcca cgtagccaga gacgccacct gttcgatgct aaggcttccc gtccgcgagg
      181 caaagggtgg gcgccgcctc ggccggcttg ggataatggc gcctgctcgg atgcggcaaa
      241 tctccgcggg agtgcgctgg cctcccattc tgggaaaaga gatcgacgat gtcgatttca
      301 ctgctgcctg ttcgatgcat taatagagca aggaaacatc cggtcgatct cgaccggatg
      361 ccgggctcga aaattcacaa ccagagggag gtacgacaat gcgattcatg gtgatagtca
      421 aagccaacga ggactcagaa gcgggtgccc tgcccagcaa ggaactcttc gccgagatgg
      481 ggaagttcaa cgaagagctg gtaaaagccg gcgtgctcct ggccgccgat ggactgcagc
      541 cgagctcgaa gggcgcgcgg gtcaagttct ctggacagca gcgcaccgtg atcgacggac
      601 ccttttccga gacgaaagaa ctgatcgccg gctactgggt gtggcaggtg aagtcgaagg
      661 aagaggcgat cgaatgggcc aagcgcatcc ccaatcccac cggcgacgag agtgaaatcg
      721 agatccgcca ggtgttcgag cctgagcgtt ccgacgcgcc gaactagcgg cggcacaaac
      781 caatgtccag ccagtctcca tgcgtacgac gaagaaacgg aacgcgggcc tgggccgcca
      841 tgacctcggt caatcaccgc gcttggtaac acagcagttc aagacaagga gcacaacatg
      901 accacgaccg tccgaacccc gccagcgcat gcgccgccgg ccagaatccc gatgacctca
      961 ctgaggaaga ccgcgctcgt cgcgggcatc ttctacctga tcaccttcat ctcgatcccc
     1021 accctcgccc tctacgtccc agtgagaaac caccgggact ggatcctcag ctccggcggc
     1081 cacaccgcgg tgctcgtggg cggcttgctg gaagtgatcg tcgccctggc cggcatcggc
     1141 accgccgtca ccttgtaccc ggtcctcaag cggcagcacg aaggcctcgc gctcggcttc
     1201 gtcacctccc gcctcctaga aggcagcatg atcttcaccg gtgtcgtcag cctgctgtca
     1261 ctggtgacct tgacgcacgg cccgggcggc gcggccggcc caaacgcggc cgcgctcctc
     1321 gccaccggcg cctcccacgt cgcggtctac aactggacat tcctgctcgg gcagagcctc
     1381 atgccagcca tcaacgcaat actgctgggc accctgctgt accggtcccg gctcgtgccc
     1441 cgcgccctgc ccgtgctagg actcatcgga gcccccctgc tcctcaccgc cgacatcgcc
     1501 ac
//
LOCUS       DASZMC010000078         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_312538,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000078 DASZMC010000000
VERSION     DASZMC010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312538"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..847
                     /locus_tag="VG274_01480"
     CDS             149..847
                     /locus_tag="VG274_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWE05347.1"
                     /translation="MTNNLPMPRVLIVDDEPPIRRFLRTSLSSQGYQVIEAEDGPCAR
                     TILRHNMADVLVLDLGLPGLDGFEIIKQLRDSGSALPIIVLSSRTDEAGKVRALDLGA
                     DDYVTKPFGMDELLARLRAALRHRLQQQGERPIFRVDQLAVDLVRRVVTVQEHEVKLT
                     PREYDLLRTLVAHAGKVLTHRFLLREVWGSETDVAYLRIYVRTLRQKLEADPDRPQLI
                     TTEQGVGYRLKAPE"
     gene            902..1846
                     /locus_tag="VG274_01485"
     CDS             902..1846
                     /locus_tag="VG274_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWE05348.1"
                     /translation="MPRELPPRTLSRGEVRIRVSACAVCRTDLHVVDGELADPKLPIV
                     PGHEIVGRIEETGADVEAMKTGDRVGVPWLGWTCGVCEFCRHGQENLCPNARFTGYQI
                     DGGFAEETVADARYVFPLPDAYADDEAAPLLCAGLIGWRALKAAGDAPRLGFYGFGAA
                     AHLAVQAARHRGQGVYGFVRPGDAKAKAFARAMGAVWAGDSDAPPPVPLDSAIIFAPV
                     GELVPAALRAVRAGGRVVCGGIHMSDIPSFPYEILWRERSVSSVANLTRTDAKEFLEL
                     ARAMKFRIETTRYRLADANRALSDLRTGALSGAAVLVP"
     gene            complement(1874..2266)
                     /locus_tag="VG274_01490"
     CDS             complement(1874..2266)
                     /locus_tag="VG274_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE05349.1"
                     /translation="MHESHEAGTLKGRKLVQVALTTRDMERSKRFYRDVLGLPLLFEA
                     GGMMFFDLQGTRLLIGTENAGHAPGGSVLYFDAPDIDRLAPALEEKGVEFLGPVQVVQ
                     RTDTHELKIRAFRDPDGNPLELMGMVPR"
     gene            2393..2929
                     /locus_tag="VG274_01495"
     CDS             2393..2929
                     /locus_tag="VG274_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HWE05350.1"
                     /translation="MNNAIKLALAASFGVMGVQTAAAQMPAAPAMSMPEGNGGMGHHG
                     MGMMHDAPFMMLLKSANLSAAQHAQVRQILQSEHAQTKPLMKQFHALHQQIAGRLLAT
                     GPLGAADLAPLTKQAFKLQQQLDQSMIDTALAIRNVLTPEQINRLSQVHQQLSNLHQQ
                     IQNLMGPEQNDANADQPE"
     gene            2951..3526
                     /locus_tag="VG274_01500"
     CDS             2951..3526
                     /locus_tag="VG274_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWE05351.1"
                     /translation="MTRRPSAENGREPPPLAELILRGCGGDRQALEVLIAHYQERVAG
                     FVFAQTQDANHCEDLCQAIFVKMVIALPLLRALDRFEPWLFQIARNACRDHLRARQSW
                     RRLFVAYQPAHESVAVHDPPEGDRREGAVEHGAALLPPEQGNLLRLSLERQRSYEELA
                     RLANTSVAAVKSRLHRARRNLRGILLAGDPE"
BASE COUNT          623 a         1162 c         1146 g          638 t
ORIGIN      
        1 cgggtaccgg attgggcctt gcgatctgcc ggggctttgt cgaggcgatg ggcgggacga
       61 tccgtgccgc caatcgaagc gacagacgcg gcgctctgtt cggcatcacg ctgccggttc
      121 ctgccgaaca gccgcgcgag cgcaccgcgt gacgaacaat ctgcccatgc cgcgcgttct
      181 cattgtcgac gacgagccgc cgatccgccg gttcctgcgc acgagtcttt cgtcgcaggg
      241 ctaccaggtg atcgaggcgg aagacggccc gtgcgcgcgc acaatattgc ggcacaacat
      301 ggccgacgtt ctcgtgctcg atcttggact gcccgggttg gacggctttg aaatcatcaa
      361 gcagttgcgc gactccggtt ccgcgctgcc gatcatcgtt ctgtcgagcc gcaccgatga
      421 ggccggcaag gtccgcgcgc tcgacctcgg tgcggatgat tatgtcacca agcccttcgg
      481 catggacgag ttgctcgctc gtttgcgcgc cgcactgcgt catcgcctgc agcagcaggg
      541 cgagaggccg attttccgcg ttgaccagct tgcggtagat ctcgtgcgcc gtgtcgtgac
      601 cgtgcaggag cacgaggtga aacttacgcc gcgcgagtat gaccttctgc ggacgctcgt
      661 cgctcatgca ggcaaggtgc tcacacaccg atttctgctg cgcgaagtgt ggggttcgga
      721 aaccgatgtc gcttaccttc ggatctatgt ccgcacgctc cgccagaaac tcgaagccga
      781 tccggatcgc ccgcagctga ttacgaccga acagggcgtg ggctaccggc tcaaggcgcc
      841 ggagtaaaaa agtgttttga cgatgcgcgc gatggttctg gaaaaaccgg gaaccccact
      901 cgtgccaaga gagctcccgc cgcgcacgct ttcgcgtggc gaggttcgca ttcgcgtatc
      961 ggcctgcgcg gtgtgccgca ccgatcttca cgtcgtcgat ggcgaactgg ccgatcccaa
     1021 attgcccatc gtgcccggcc acgaaatcgt gggccgcatc gaagagaccg gcgcagacgt
     1081 cgaagccatg aaaacgggcg atcgtgtcgg tgttccatgg ctcggctgga cctgtggcgt
     1141 gtgtgagttc tgccggcatg gccaggagaa tctgtgcccc aacgcgcgct tcacgggcta
     1201 ccagatcgat ggaggcttcg ccgaggaaac cgtcgccgac gcgcgctacg tctttccgct
     1261 cccggacgcc tacgccgatg acgaagcggc gccgcttttg tgcgcggggc tgatcggctg
     1321 gcgtgcgctc aaggccgcgg gcgacgcgcc gcggctcggc ttctatggct tcggcgccgc
     1381 cgcgcatctt gcggtccagg cggcgcggca ccgcggtcag ggcgtctatg gattcgtgcg
     1441 ccccggcgac gcaaaggcga aggcgtttgc gcgcgcgatg ggtgcggtgt gggcgggaga
     1501 ttccgatgca ccgccgcccg tgccgctcga ctcggcgatc atttttgcac cggtgggaga
     1561 gcttgtgcct gcggcgttgc gcgcggtgcg cgccggcggc cgcgtcgtct gcggcggcat
     1621 ccatatgagc gatattccgt cgttccccta cgaaatcctc tggcgcgaac gttcggtgtc
     1681 gtccgtcgcc aatctcacgc gcaccgatgc caaggaattt ctcgaactgg cgcgcgcgat
     1741 gaagttccgc atcgaaacca cccgctatcg gcttgccgac gcgaaccgcg ccctttccga
     1801 tcttcgcacc ggcgcgctga gcggcgcggc agtgctcgtt ccgtagcgga cggcgcaaac
     1861 ggcgggacgg gtttcagcgc gggaccatgc ccatcaattc gagcggattg ccgtcggggt
     1921 cgcggaaggc gcgaattttc agctcgtgcg tgtcggtgcg ctggaccacc tgcacaggcc
     1981 ccaggaattc gacgcccttc tcctcgagcg ccggcgcaag ccgatcgata tcgggcgcgt
     2041 cgaaatagag aaccgaacct cccggcgcat gtccggcgtt ttctgtgccg atcagaagac
     2101 gcgtgccctg cagatcgaag aacatcatgc cgccggcttc aaagagcagc ggcagcccga
     2161 gaacatcgcg gtagaaccgc ttgctgcgct ccatgtcgcg cgtcgtgagc gcaacctgga
     2221 cgagtttccg gcctttcagc gtgccggctt cgtgcgattc gtgcatcgct ggcatccttc
     2281 gcgtgataga cttggctcat cccaacatgc gcagcggcgg gatgaatagc gaaactttca
     2341 ggggagcgct ccgtctttgt ggtatccacc gttcaaaccg gagaacatct tcatgaacaa
     2401 cgcaatcaaa ctggccctcg ccgcttcatt cggcgtgatg ggcgtgcaga ccgcggccgc
     2461 ccagatgccg gccgcccccg ccatgtccat gccggagggc aatggcggca tggggcatca
     2521 cggcatgggc atgatgcacg atgcgccctt catgatgctt ctgaaatcgg ccaatctgag
     2581 cgcggcccag cacgcccaag tccggcaaat cctccagtcg gagcatgccc agaccaagcc
     2641 gctgatgaag cagtttcacg cccttcatca acagattgcg ggccggcttc tcgctaccgg
     2701 gccgctcggc gccgccgatc tggcgccgct gacgaagcag gctttcaagc tgcagcagca
     2761 gctcgaccag agcatgatcg acacggcgct tgccatccgc aacgtcctca cgcccgagca
     2821 gatcaaccgc ctctcgcagg ttcaccagca gctcagcaat ctgcaccagc agatccagaa
     2881 cctcatggga ccggagcaga acgacgcgaa cgcggaccag cccgaatagt ttggcgacag
     2941 gctgcgcccc gtgactcgcc gccccagcgc ggaaaacggc cgggaaccgc cgcccctcgc
     3001 ggagctgatc cttcgcgggt gcggcggcga ccggcaggcg ctcgaggtgt tgatcgcgca
     3061 ctatcaggag cgcgtggcgg gtttcgtgtt cgcacagacg caagacgcga atcattgcga
     3121 agatctgtgc caggccatct tcgtgaagat ggtgatcgcg ctgccgcttc tgcgcgcgct
     3181 tgaccgcttc gagccgtggc tgttccagat tgcgcgcaat gcgtgccgcg accatctgcg
     3241 cgcccgtcaa tcctggcgca ggctcttcgt ggcctaccag ccggcgcacg aatccgtcgc
     3301 ggtgcacgat ccgccggaag gcgacaggcg cgaaggcgcg gtggagcatg gcgcggcgct
     3361 cctgccgccg gaacaaggca acctgctccg gctctcgctc gagagacaaa ggagctatga
     3421 agaactggcg cggcttgcaa ataccagcgt tgcggcggtg aaatcgcgtc tgcaccgcgc
     3481 gcggcggaac ttgcggggaa tcctgttggc cggagatccg gaatgaacga aatgcatctg
     3541 accccacctc gtctcgcaac cgatttcgc
//
LOCUS       DASZMC010000079         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_35324,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000079 DASZMC010000000
VERSION     DASZMC010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35324"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VG274_01505"
     CDS             complement(<1..1162)
                     /locus_tag="VG274_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE05352.1"
                     /translation="MRARVLPGAVACGTHDRNVDDLTLCFRSVRHPALREAGEALRAN
                     RNEAAERTLLRFLDRHPDNVDGLYLMAEAAARSGRFADSERLLARSIEREPAFDAARF
                     AYAKVLHEQGKSYLSEMQLEALSLNDPRNAQYLGLRALVLAALGKHDAALDCCRKLME
                     RHPNSGEFQIRYGHALRAMGLRDECISVYRRVVESFPARGDAYWGLASLKAFRFTSAE
                     IDAMQALLGRSDLTSENRVYLHFALGRALGDLAQWEKSFENYARGNAIRRIDVAYDPD
                     DSTKHASAIRKNFTPAWIQAHAGGGCALREPIFVLGMQRSGSTLVEQILASHSAIEGM
                     GELPEYPLLASCRADGGIAGPEDGDMLDPENLVNRRISGERYLENVRRRRKFV"
     gene            complement(1241..1429)
                     /locus_tag="VG274_01510"
     CDS             complement(1241..1429)
                     /locus_tag="VG274_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05353.1"
                     /translation="MNQLAPACKERVPKLQALFEFGQKQLAKNKAAIDKAKAEDEKDD
                     AAAAGAAAAKTASPPAHK"
BASE COUNT          407 a          630 c          623 g          369 t
ORIGIN      
        1 ccacgaattt ccgccgtcgg cggacgtttt cgagatatcg ctcgcccgaa attcgccggt
       61 tgacaagatt ctccgggtcc agcatatccc catcttccgg accggcgatc ccgccatccg
      121 cgcggcacga tgcaagcagc ggatattcgg gcagctctcc cattccttcg atcgccgaat
      181 ggcttgccag aatctgttcg accagcgtgg aaccggagcg ctgcatgccc aggacaaaaa
      241 tcggctcgcg caaagcacat ccgccgccgg cgtgcgcctg aatccaggcg ggcgtgaagt
      301 tcttccggat cgccgacgcg tgcttcgtcg aatcgtcggg atcgtaagcg acatcgatcc
      361 ggcggatggc gttgcctctg gcgtagtttt caaacgattt ttcccattgc gccagatcgc
      421 caagcgccct gccgagggca aaatgcagat aaacgcgatt ttcagatgtc agatcggagc
      481 gaccgagcag ggcctgcatg gcatcgattt cggcactggt gaagcgaaac gctttcaggc
      541 tcgcgagccc ccaatacgcg tcgccgcgcg cggggaaaga ctcgacgacc ctgcggtaaa
      601 ccgaaataca ctcgtcgcgc agacccatgg cgcgaagcgc atggccgtag cggatctgaa
      661 attcgccgga attgggatgc cgctccatca gctttctgca gcagtccaat gcggcgtcgt
      721 gctttccgag cgcggccagg accaaagccc gcaggccgag atattgcgcg ttccgtggat
      781 cgtttagcga aagggcttcg agctgcatct ccgacaggta cgacttgccc tgttcatgaa
      841 gcaccttcgc ataggcaaat cgggccgcat cgaatgcggg ctcacgctcg atgcttcgcg
      901 cgagcaacct ttcggagtcc gcgaagcggc ccgaacgcgc agcggcttcg gccatgagat
      961 agaggccgtc gacattatcg ggatgccggt cgagaaagcg gagcagcgtg cgctctgcgg
     1021 cctcgttgcg attggccctc agtgcttcac ccgcctccct taatgccgga tggcgtaccg
     1081 aacgaaagca gagcgtaaga tcgtcgacgt tcctgtcatg ggtgccgcag gcgacggctc
     1141 ccgggagcac gcgcgcacgc atcagcgtga cgcccggtaa acgcctctcg atcgaaccgg
     1201 cgtcaaaagc gagcgccgac ggtggcgcga gccctcgcca tcacttgtgc gcaggtggtg
     1261 aggccgtctt cgccgcagca gcacctgccg cggcggcatc gtctttttcg tcctcggctt
     1321 tcgccttgtc gattgcggcc ttgttcttcg caagttgttt ctgcccgaat tcgaacaggg
     1381 cctgcaattt cggcactcgt tccttgcagg caggcgccag ttgattcatg tgctgcgcca
     1441 gacacgcgat ccggcgtcct tcgccgtaag gtacattcgg acagaaacgc tgaaggtccg
     1501 gtacgcaagg accatgcccc ggagttggcg gcggcggcgg attgctcgca gtgaattgag
     1561 caaaagccga cgccgtggaa agcaacagga caacaccaat gacggcaact cgcatcgcat
     1621 agaaactcgc aaggttcgag cgcacattaa tctcaaacct tcgtcgtgcc aagccggggg
     1681 gcggcgggca ttccgggaac tacgcatcgg ctatgccggg aaacccggtt ccatcgacgc
     1741 cgatggaagc ttgcgctgga ctcggcgccg gaggagcgct agtaagacgc ccctccggct
     1801 ggcgcgaaac cttcgagaca agaacggggt tggtgacaat tgatcgcggt cccggggctg
     1861 gcgagcaggc gatcggacca atggaacacg cacagatcaa cgcccatgat ggcgagccgc
     1921 tctggagcat tctggcgcag tcggtggcga tccttgagga cgatcccgac gaagccgaac
     1981 ggcatgtgaa ggccgtcctc aaacaggctc ccggtcatca gcaggcgct
//
LOCUS       DASZMC010000080         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_312872,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000080 DASZMC010000000
VERSION     DASZMC010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312872"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VG274_01515"
     CDS             complement(<1..526)
                     /locus_tag="VG274_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05354.1"
                     /translation="MTVQADTLVIFGATGDLARRMLLPSLYFLQRDGLLDERLRIVGA
                     ARSDLDRDRFRALVRETVEERAGKPDEQAWLALANRLDYCRLDASQQQDYPALGEFVG
                     KSPVFYLSLSPQHYSAACANLRAVGLATAHSRIVVEKPIGHDLESCRAINACLAQAFD
                     ERRVFRIDHYLGKET"
     gene            complement(647..>2310)
                     /gene="ligA"
                     /locus_tag="VG274_01520"
     CDS             complement(647..>2310)
                     /gene="ligA"
                     /locus_tag="VG274_01520"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWE05355.1"
                     /translation="TRGDGLEGEDVTANLRTLRDVPLRLHGKAPDIVEVRGEVYMLHN
                     DFAALNARQAEAGRPLFANPRNSAAGSLRQLDPAITARRPLHFFAYAWGELSRLPASS
                     QFELLEAFREWGFRVNPLVRRCRTTGDLLEFYRDMETRRATLGYDIDGVVYKVDRLDL
                     QERLGFVSRSPRWAIAHKFAAEQADTVLVDIDIQVGRTGTLTPVAKLKPVTVGGVVVQ
                     NATLHNEDEIARKDVRIGDTVVVQRAGDVIPQIVRVIAEKRPRGAKPYVFPDECPVCH
                     SHAVREIDEKTGKPEAARRCTGGLICAAQAVERLRHFVSRDAFDIEGLGEKHIRSFHE
                     DGLIKEPADIFLLAKRHGSGDKAIARREGWGEQSASKLFEAIDRRRKIGLDRFINALG
                     IRHVGETTAKLLARNFGTLDAFIAAMESEDALQELDAIAGIGETVAEAIRDFFDEPHN
                     RKAVDHLLKEVEVDPVASARTAHSAVAGKTVVFTGSLEKMTRPEARARAEALGAKVAG
                     SVSKKTDLVIAGPGAGSKLGEAEKLGVKVIDEGAWLKLIGGARSA"
BASE COUNT          405 a          748 c          725 g          432 t
ORIGIN      
        1 ccgtttcctt gccgagataa tggtcgatgc ggaagacgcg ccgttcgtcg aaggcttgtg
       61 cgagacaggc gttgatcgcg cggcagctct caaggtcatg gccgatcggc ttttccacca
      121 cgatgcggct gtgtgccgtg gcgaggccca cggcgcgcag attggcgcag gcggcagaat
      181 aatgctgcgg ggagagagac aggtagaaaa cgggactctt gccgacgaat tcgcccaaag
      241 caggataatc ctgctgctgg gacgcatcga gcctgcagta atcgagccga ttcgccagcg
      301 cgagccacgc ctgttcgtcg ggttttccgg cgcgctcctc gaccgtttcc cggaccaatg
      361 cgcgaaagcg atcccgatcc agatcggaac gcgcggcgcc gacgatccgc aggcgctcgt
      421 ccagaagccc gtcgcgctgg agaaaataca gcgacggcag cagcatccgc ctggccagat
      481 cgccggtggc gccaaagatc acgagggtgt cagcttgaac ggtcatatcc agggcacgtc
      541 atcacgtcat gaggtatacg ccgcaacatg aggatcacca cggactcaac gcgcccaggt
      601 tgcaatcctt tccgcgccag ctgcgccatg gcttcggaac aaacaatcac gcggatcgcg
      661 ccccgccgat caacttcagc caggcgccct cgtcgatcac tttcacgccg agtttttcgg
      721 cttcgccgag cttcgagccg gcgccggggc ctgcgatgac gagatcggtt ttcttcgaca
      781 cggagcctgc tactttggcg cccagcgctt cggcgcgcgc cctggcttcg ggccgcgtca
      841 tcttttcgag cgatccggtg aacacgacgg tcttgcccgc gaccgcggaa tgcgcggtcc
      901 tggccgatgc gaccggatcg acttcgactt ccttcagcag atgatcgacc gccttgcgat
      961 tgtgcggctc gtcgaagaaa tcccggatgg cctcggcgac ggtctcgcct atgccggcga
     1021 tcgcgtcgag ttcctgaagc gcgtcttcgc tctccatcgc ggcaatgaat gcatcgagcg
     1081 tgccgaaatt gcgggcgagc agctttgccg tcgtctcgcc cacatggcgg atgccgagcg
     1141 cgttgatgaa gcggtcgagc ccgatcttcc tgcgccggtc gatagcctcg aagagcttgg
     1201 acgcgctctg ctcgccccag ccttcccgcc tggcgatcgc cttgtcgccc gaaccgtggc
     1261 gctttgcgag cagaaaaatg tccgcaggct ccttgatcag gccgtcttcg tgaaaactcc
     1321 tgatgtgctt ctcgccaagt ccctcgatgt cgaaggcgtc gcgcgagacg aaatgccgga
     1381 gcctctcgac ggcctgggcg gcgcagatga gcccacccgt gcagcgccgc gccgcttccg
     1441 gctttcccgt tttctcgtcg atctcgcgca ccgcatggct gtgacagacc ggacattcgt
     1501 ccggaaacac atagggtttt gcgccgcggg gccgcttctc cgcaatgacg cgcacgatct
     1561 gcggaattac gtcgcccgcg cgctgcacga ccaccgtgtc gccgatgcgc acatccttcc
     1621 tcgcaatctc gtcttcgttg tgaagcgttg cattctgcac gacgacgccg ccgaccgtta
     1681 cgggcttgag cttcgccacc ggcgtgagcg tgccggtgcg gccaacctgg atgtcgatgt
     1741 cgacgagaac ggtgtcggcc tgttcggcgg cgaatttgtg cgcgatcgcc cagcgtggag
     1801 agcgcgagac gaatcccaac cgttcctgca gatcgagacg gtcgaccttg tagacgacgc
     1861 cgtcgatgtc atagccgagc gtggcccgcc ttgtttccat gtcgcgatag aattcgagca
     1921 ggtcgccggt cgtgcggcag cgccggacga gcggattgac gcggaagccc cattccctga
     1981 acgcttcgag caattcgaat tgggaagaag cgggaagccg gctcaattcg ccccaggcat
     2041 aggcaaagaa gtgcagcgga cgccgtgcgg tgatggcagg atcgagttgg cgcagcgaac
     2101 ccgccgcgga gttgcgcgga ttcgcgaaga gcggcctgcc agcctctgcc tgccgcgcgt
     2161 tcaatgcagc aaaatcgttg tgcagcatgt agacctcgcc gcgcacttcg acgatgtcgg
     2221 gcgctttgcc gtgcaacctg agcggcacgt ccctcagcgt gcgcaaattc gcggtgacgt
     2281 cctcgccttc gaggccgtcg ccgcgggtcg
//
LOCUS       DASZMC010000081         7042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_596299,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000081 DASZMC010000000
VERSION     DASZMC010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596299"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VG274_01525"
     CDS             <1..614
                     /locus_tag="VG274_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05356.1"
                     /translation="DERRRPADILAYADVKPGETVGEFLPGGGYYTRMLSDVVGPTGK
                     VYALETTTWGKDNIASTKAVLSEPGRGNVKLDLAPLGTFRLPEKVDLFWTTNNYHDLH
                     IPKYANVDMVRFNKLVFDSLKPGGTYFIVDHAAAAGTGASDSPTLHRIEKATVLKEVT
                     SAGFKLVGESDALSNPSDDHRKTVFDPAIHFKTDQFILKFARP"
     gene            837..1202
                     /locus_tag="VG274_01530"
     CDS             837..1202
                     /locus_tag="VG274_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05357.1"
                     /translation="MTTPNTFLAIFLGSKTSPRMVAWNSLPEGERRAKEQEGMAAWKG
                     WIEKHQAAVVVMGGPLGKTKKVTQRGTEDTSNEMGAFMVVRADSHEAAARLFEKHPHF
                     TIFPGESVEIMPVLPIPGA"
     gene            1231..1689
                     /locus_tag="VG274_01535"
     CDS             1231..1689
                     /locus_tag="VG274_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWE05358.1"
                     /translation="MTAGPDLFETAQCLCLASRRAARAITRRFDTALRPHGIKATQFT
                     LLAVLALKGSQPIGVLAEFIGADRTTLTRNLAVAERQSLVRIGSGADARARVASITAR
                     GRQTLQRAFSVWRETQRELTTAVGMAAADSLRQISRKPPVRKASGAVHRQ"
     gene            complement(1739..2431)
                     /locus_tag="VG274_01540"
     CDS             complement(1739..2431)
                     /locus_tag="VG274_01540"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor R domain-containing protein"
                     /protein_id="HWE05359.1"
                     /translation="MIRKAVLTLAVLALAGSGASAAGPKGPVGPNRVFLMPDGMAVAT
                     RPSASHLYPRNLAPGEAPIYDNLAGKYPDGTYYCCVGWVVSGPDVPLFGQTWLAAAFT
                     PANNTSVTRVDLGIGFVSGNTNALVVTLNSDASGAPGTELAKFEIRNMPMFGSCCAVA
                     TVKDKAGTALVGGQQYWVVVKTNNKDSDIWANWNSNDTEQINPVPGAYNAGSGWITGN
                     YLVGMAFAVFGK"
     gene            complement(2497..2772)
                     /locus_tag="VG274_01545"
     CDS             complement(2497..2772)
                     /locus_tag="VG274_01545"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HWE05360.1"
                     /translation="MRRYIIERDIPKIGSAERDALRDAAQKSNSVLAELAPDIQWVES
                     YVAADKTFCVYLAKDEAMIRKHAEMSGFPATKITEVSKTIDPTTARP"
     gene            complement(2847..3494)
                     /locus_tag="VG274_01550"
     CDS             complement(2847..3494)
                     /locus_tag="VG274_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWE05361.1"
                     /translation="MTNPTPIKTVIGLLLTLGAGFLPLGAWGRTYSGLGRLVGNEVLW
                     WAAVVVILLYIAFVERRPFNSVGFRKPRILDLILAAIAGVLMVAGIVFIYYVVFPALH
                     LRMNAATMRSLLETPFWYRLILVTRAAVAEELLFRGYPIERICELTASRWLAALLTWA
                     AFTYAHLSSWGAAQLIVAGYGGVILTWLYLWRRNLWANMLAHWIADGAGFLLPHG"
     gene            complement(4007..4807)
                     /locus_tag="VG274_01555"
     CDS             complement(4007..4807)
                     /locus_tag="VG274_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWE05362.1"
                     /translation="MSASERARRFGDLHRDGLLILPNAWDAGSARIIESAGAIAIATS
                     SAAVAWSHGYPDGEALPEEALLATLREIVRVVGVPVSADIEAGYARDADAAGEFVAKI
                     LDAGAVGVNIEDGRDAPDLLCAKIERLRSVAERAGIELWINARVDVYLRQLAEGDAAY
                     AETVARARRYAQAGASSIFVPGAAEEALIGNLAREVRLPLNVLAWPGLPDASKLRVLG
                     VRRLSAGTGLGKVALGRIRELAEAFLREGRSALFAEGPADINALMRRD"
     gene            complement(4804..5349)
                     /locus_tag="VG274_01560"
     CDS             complement(4804..5349)
                     /locus_tag="VG274_01560"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWE05363.1"
                     /translation="MAEHGNNYFDRIETPIGKLVVIADAKGAMRTVWFDDGDEGWRAD
                     CERRFPGAVLTARRDPFGHASTLKAYFKGDMDALDRIPVVFSGTPFQNKVWKALRKIP
                     IGTTTSYGALAKKIGAPTAVRAVGLANGSNPVGVVVPCHRVIGSDGSLTGYGGGLPRK
                     KWLLEHEARHCASQFHLEGSA"
     gene            complement(5342..6826)
                     /locus_tag="VG274_01565"
     CDS             complement(5342..6826)
                     /locus_tag="VG274_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HWE05364.1"
                     /translation="MSNGLPPHDVCYRALSARDARFDGRMFVGVSTTGIYCRPICPAR
                     TPKRENCRFFPSAAAAQTSGFRPCLRCRPEISPDAAAWRGTSNTVSRALALIAEGALD
                     GDESGVSTLADKLGVGERQLRRLFDKHLGVPPIAVAQTRRILFAKQLIQETRLSMAQV
                     ADVSGFGSVRRFNDAFQKLYRRAPRDLRLLRSAPSEPSLVTLRLGYRPPYDWDSILSF
                     FAMRAIAGVETVERGRYRRTIALDGLAGSVEIAHGENCLVATIRLPQLRALLPVVGRL
                     RRMFDLDADVETIGAHLSGDEGLAPLIARRPGLRTPGAWDGFELAVRAILGQQITVKA
                     ARSLAGKLVQLAGSPVRPEAAGHAALTHVFPTPQQLAAADLSNLGMPRPRIAALQALA
                     RAAVANPKLFESGGAVDDVVARLLALPGFGEWTAQYWALRALRDSDAFPAADVGLLRA
                     LATNGKRLSPRALMERAENWRPWRAYAAQHLWTADGDLRSNAHG"
BASE COUNT         1388 a         2294 c         2134 g         1226 t
ORIGIN      
        1 tggatgagag acgaaggccc gccgacatcc tcgcctacgc ggacgtgaag cccggcgaaa
       61 cagtcggtga gtttcttccg ggtggcggct actacacccg catgctgagc gacgtcgtcg
      121 gcccgaccgg aaaagtttac gcactagaga cgacaacctg gggcaaggac aatatcgctt
      181 ccacgaaggc ggtgctgagc gagccaggac ggggcaacgt gaagctcgat cttgcgccgc
      241 tcggcacttt ccgcctgccg gaaaaggtcg acctgttctg gaccacaaac aactatcacg
      301 acctgcacat cccaaaatac gccaacgtcg acatggtgcg cttcaacaag ctcgtgttcg
      361 actcgctgaa gccgggcggc acctatttca tcgtcgatca cgcggcggca gcggggacgg
      421 gcgcgagcga ctcgcccaca cttcatcgca tcgaaaaggc gaccgtgctc aaggaagtaa
      481 cctcggccgg attcaagctc gtcggcgaga gcgatgcgct cagcaatccg tccgacgatc
      541 acaggaaaac cgtcttcgat cccgccatcc atttcaaaac ggatcagttc atcctcaaat
      601 tcgcccggcc ctgatcagca taatgccggc ttcgctgcgg cccctgacgg tgcgcgctga
      661 aaccagcact gcacgcagtt cctcgcggac cgtgactcga agcgcggtcg aggtgcgcct
      721 gttgacgtga cttgacggtg cgagataggt gtatctacac tctctgcatg atcaccgggc
      781 cggagctgtg gaaagtcgcc gttttgtttt gcaacacgta aagagaagga atcctcatga
      841 cgacgcccaa tacatttctc gccattttcc ttggcagcaa aaccagcccg cgcatggtag
      901 catggaatag ccttcccgaa ggggaacggc gcgccaagga acaggaggga atggctgcct
      961 ggaagggctg gatcgaaaag catcaggccg cggtcgtggt catgggcgga ccgctgggca
     1021 agaccaaaaa agtcacgcaa cgcggcaccg aagataccag caatgagatg ggtgccttca
     1081 tggtcgttcg cgcagactcg cacgaggccg ccgccagatt gttcgaaaag catccgcact
     1141 tcacgatctt tccgggcgaa tccgtggaga tcatgccggt tctgccgata ccgggcgcct
     1201 agggcccggt atcggaccgg cacgcgtttc atgacagccg ggccggacct gttcgaaacc
     1261 gcgcaatgtc tttgcctggc gtctcggcgg gccgcgcggg ccatcacgcg ccggttcgat
     1321 acggcgttgc ggccccacgg catcaaggcg acgcagttca cccttctggc cgtactcgcg
     1381 ctcaagggct cccaacccat cggcgtgctc gcggaattca tcggcgccga ccgcaccacg
     1441 cttacgcgca acctcgcggt ggccgagagg cagtcgctgg tgcggattgg atcgggagcg
     1501 gacgcccgcg cgcgcgtcgc ctccatcacc gcgcggggac gccagacatt gcagcgcgcc
     1561 ttttcggtct ggcgggagac gcaacgcgaa ttgaccaccg ccgtcggcat ggctgcggcc
     1621 gacagcttga ggcagatttc gcgcaagccc ccggttcgca aagcttcagg ggcggttcat
     1681 cgacaatgac gattgccgct tcgccccccg gggacaatcg tccgaccgga accccgtgct
     1741 atttcccaaa gacggcaaac gccatcccga cgagataatt cccggttatc caccctgatc
     1801 cggcgttgta ggcgcccggg accggattga tctgctcggt gtcatttgag ttccagttcg
     1861 cccaaatatc cgagtccttg ttgttggtct tcacgacgac ccagtattgt tgcccgccga
     1921 cgagcgccgt gccggccttg tccttcaccg tggccacggc gcagcagctg ccaaacatcg
     1981 gcatattcct tatctcgaat ttcgcgagtt ccgttcccgg tgcgccgctg gcgtcactgt
     2041 tgagtgtgac gacaagagca ttggtgtttc cgcttacgaa gccaattccg agatcgaccc
     2101 gcgttacgga agtgttgttc gccggggtaa acgctgcggc tagccaagtc tgtccaaaca
     2161 ggggcacgtc tggcccgctg accacccagc ccacgcagca ataatacgtg ccgtcaggat
     2221 atttcccggc gagattgtca tagatcggcg cttcgcccgg cgccagattt ctgggatata
     2281 agtgagatgc ggagggcctc gttgcgaccg ccattccgtc cggcatcaga aaaacccggt
     2341 ttggaccgac cggtcctttt ggacccgccg ccgaagctcc cgaaccggcg agcgccagaa
     2401 cggccaatgt caaaactgcc tttcgaatca cgccatcctc ctgccctgga acacaataga
     2461 ccagctgtta aaactgaacc accaaataac ggacgctcag ggccgtgcgg tggtcggatc
     2521 gattgttttg gagacttcgg tgatcttcgt cgccggaaag ccgctcatct ccgcatgctt
     2581 gcggatcatg gcctcgtcct tcgccagata gacgcaaaac gtcttgtccg ccgcaacata
     2641 gctctcgacc cattgaatgt cgggcgccag ttcggccaac acggaattcg atttctgtgc
     2701 cgcatctctc agtgcgtcgc gctcggcgct gccgatcttc ggaatatcgc gttcgatgat
     2761 gtatcttcgc atgatgcgct cctcctgcct gatcggaggt taggacacaa gcattccccc
     2821 ctcaagtgag gcttgggccc gcgggctcag ccgtggggta gcaggaagcc tgcgccgtcg
     2881 gcgatccagt gcgcgagcat gttagcccac agattgcgac gccagagata gagccaggtc
     2941 agaatgacgc caccatagcc cgccacgatc agctgcgctg cgccccagct tgacagatgt
     3001 gcataggtga aggcggccca agtaagcagc gccgcgagcc agcggctggc ggtgagttcg
     3061 catatcctct cgatcggata gccgcggaac agcaactcct cggcaacggc cgcacgcgtc
     3121 acgaggatta gccggtacca gaagggagtc tcgagcaagg aacgcatcgt ggcggcgttc
     3181 atcctcagat gcagcgcggg gaacacgacg tagtagatga acacgatacc ggcgaccatc
     3241 agaacgccgg cgatcgccgc gaggatgagg tcgagaattc ttggcttgcg aaatccgacc
     3301 gagttgaaag ggcgccgctc gacgaacgcg atgtaaagca gaatgacgac aaccgcggcc
     3361 caccacagga cttcgtttcc gaccagtcgg ccgagcccgc tataggtgcg gccccaggcg
     3421 ccaagcggca gaaaaccggc gccgagcgtc aacaggagac cgatgacggt cttgatgggt
     3481 gtcgggttgg tcaccgcttc agttcaacgc ttcgacatca tttggcgatg cgccgcggac
     3541 gcggcacaag ctcgccgccg caattggggc agcgattgtt catcgtttcc gaacacgcgc
     3601 cacaaaaggt gcattcgtag gagcaaatcc gtgcgtggtc gctgtccttc ggcagcggcg
     3661 cgctgcaacg ctcacaagct tcccgcatct cgagcgccat cgacgatctc ctccgcgcgg
     3721 gcaaacgtga gcacacattt tttcatgttt cgtgatactg tgcacgacct cgttttcctc
     3781 ccccatcgtg ggggaggtgg tttgcgcagc gaagcaaacc ggagggggga gtccgcgcgc
     3841 ggcgcgcgcg ccacgtcccc ctccgcctcg ctgcgctcgg cacctccccc acgatggggg
     3901 aggaaaatca agcgcgcagc atggctcgat ttctatgttg aacagctcgg gcgcattcat
     3961 gaaatgatgt gtgctcacgt tagccgtgcg agacgaagct ctcagatcaa tcgcgccgca
     4021 tcagcgcgtt gatgtcggcc ggaccctccg cgaacagcgc agagcgcccc tcgcgtagaa
     4081 acgcctcggc cagttcgcgg atccggccga gtgccacctt gccaaggccg gtgcctgcgc
     4141 tcagccgccg cacgccgagc acccttagct ttgacgcgtc gggaagcccc ggccaggcca
     4201 gaacattgag aggaagtctc acttcgcgcg cgagatttcc gataagcgcc tcttcggcgg
     4261 cgcccggaac gaagatcgaa ctggcgcccg cctgcgcata gcggcgggcg cgggccaccg
     4321 tttcggcgta ggccgcgtca ccctccgcga gctggcgcag atacacgtcc acccgcgcat
     4381 tgatccacaa ctcgatgccg gcgcgctcgg caacgcttcg gagccgctcg atcttggcgc
     4441 acaacagatc cggcgcgtcg cgcccatcct cgatattcac acccaccgcg cccgcatcga
     4501 gaattttcgc tacgaattcg ccggcggcat cggcgtcgcg tgcgtaaccc gcctcgatgt
     4561 ccgccgacac cggaacgccg accacgcgca cgatctcacg cagcgtcgcg agcaacgcct
     4621 cctccggcag cgcctcgccg tcgggatatc cgtgcgacca cgcgacggcg gcgctcgacg
     4681 tggcgattgc tatcgcgcct gcggattcga tgatccgcgc gctacctgcg tcccaggcat
     4741 tcggcagaat cagcaaacca tcgcgatgaa gatcgccgaa ccggcgcgcc ctttcagatg
     4801 cactcacgcg gagccctcca gatggaattg cgacgcgcaa tgccgcgcct cgtgttcaag
     4861 cagccacttc ttgcgcggca acccgccgcc gtaaccggtg agcgagccat cgctgccgat
     4921 cacacgatgg cacggcacga cgacgccgac gggattggag ccattcgcga gaccgacagc
     4981 ccgcacggct gtgggcgcgc cgatcttttt cgccagcgcg ccgtagctcg tcgtcgtacc
     5041 aatgggaatt tttcgcaacg ccttccatac cttgttctga aacggcgtcc cggaaaatac
     5101 gacgggtatc cggtccagcg catccatgtc acctttgaaa tacgctttga gcgtcgaggc
     5161 atgaccaaaa ggatcgcgcc gcgctgtcaa caccgcgccc ggaaatcggc gttcgcagtc
     5221 cgcgcgccaa ccttcgtctc cgtcgtcgaa ccagaccgtg cgcatcgcgc cttttgcatc
     5281 ggcgatgacg acgagcttgc cgatcggcgt ttcgatacga tcgaagtaat tgtttccatg
     5341 ttcagccatg agcgttgctc ctcaaatctc catcagccgt ccacagatgc tgcgccgcat
     5401 aggcgcgcca gggccgccag ttttccgcgc gttccatcag cgccctgggt gacagccgtt
     5461 tgccgttggt cgcgagggcg cgcagcagac ccacatccgc cgcggggaac gcatcgctgt
     5521 cgcgaagcgc ccgcagcgcc caatattgcg cggtccactc gccgaagccc ggtagcgcga
     5581 gaagacgcgc cactacgtcg tcgaccgcgc caccggactc gaacagtttc gggttcgcga
     5641 cagcggcgcg cgccagcgct tgcagcgccg cgattcgggg gcgcggcatg ccgaggttcg
     5701 aaagatcggc tgccgcaagc tgttggggcg taggaaagac atgtgtcagc gccgcatggc
     5761 ccgccgcttc gggacggacc ggcgaacccg caagctggac gagcttgccc gcaagcgagc
     5821 gcgctgcctt gaccgtgatc tgctggccga ggattgcacg cacggcgagc tcgaagccgt
     5881 cccaggcgcc gggggtgcgc agccccggcc gccgcgcgat cagcggcgcg aggccctcgt
     5941 ctcccgacag atgcgcgcca atcgtttcga catcggcatc gagatcgaac atgcggcgca
     6001 gccggccgac caccgggaga agagcgcgca gttgcggcag ccggatcgtt gccaccaggc
     6061 aattttcgcc gtgcgcaatc tcgacgcttc ccgccaaacc atcgagtgca atcgtgcggc
     6121 ggtaacgtcc gcgttcgaca gtctcgacac cggcgattgc gcgcatcgca aagaatgaga
     6181 ggatggagtc ccaatcgtag ggcgggcgat agccgaggcg taaagttacg agagaaggct
     6241 ccgaaggcgc actgcgcagc agcctgagat cgcgcggcgc gcggcgatag agtttttgga
     6301 aggcatcgtt gaagcggcgc acgcttccga agcccgaaac gtccgcgacc tgcgccatcg
     6361 aaagtcgcgt ctcctgaatc aattgcttcg caaatagaat tcgccgggtc tgcgccacgg
     6421 caatcggcgg cacgcccaga tgcttgtcga acaggcggcg caactggcgt tcgccgacgc
     6481 cgagcttgtc ggcgagcgtc gaaacccccg actcatcgcc gtcgagcgcg ccttcggcga
     6541 tgagcgccaa agcgcgggaa accgtatttg aagtcccacg ccaggcggca gcgtcgggag
     6601 aaatctcggg acggcagcgc aggcatggcc ggaagcccga tgtctgcgca gccgcggcgc
     6661 tgggaaaaaa ccggcaattc tcgcgcttgg gtgtgcgcgc cggacagatg ggacggcaat
     6721 agatgccggt cgtcgaaacg ccgacgaaca tgcgcccgtc gaaccgcgcg tcgcgggccg
     6781 acagcgcgcg gtagcagacg tcatgaggag gaaggccgtt gctcatgaag cggactatgc
     6841 acgagcggcc catccgagtc tggccatttt cggacgtgac cgtcaggcct gtctccttcc
     6901 gcgacaatgc gaaattcttt ctggagctcg ctccgctcat gtaaaatcaa aacatgtcat
     6961 ccccgcgcaa gcgctagcgc atctccacat cgtgtacgcc tattttttcc tcccccgcgc
     7021 gaatgcgcgg gggaggggga cc
//
LOCUS       DASZMC010000082         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2519024,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000082 DASZMC010000000
VERSION     DASZMC010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519024"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..367
                     /locus_tag="VG274_01570"
     CDS             179..367
                     /locus_tag="VG274_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05365.1"
                     /translation="MPRIKHKDHNEFSGLAPYGDAKVVTPKEDFDRNRDGLYGGLPKA
                     RDKNAEGPAPNKPHPAKK"
     gene            398..1285
                     /locus_tag="VG274_01575"
     CDS             398..1285
                     /locus_tag="VG274_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE05366.1"
                     /translation="MHADLKDPTEKHPKPPFKKQKQPWPGLARDMEPPPDHGEKSYKG
                     SGRLKGRKALITGGDSGMGRAAAIAYAREGADVAINYFPTEEPDAKDVIALIEDAGQK
                     GAAIPGDLRDEVFCKELVAKAIDKLGGLDIVVCNAARQQSHDSILDITTEDFDATMKT
                     NIYAPFWIIKAALPHLKPGAVIVATASEQAYDPSAELYDYAQTKAATMNYVKSLAKQL
                     ASKGIRVNGVAPGPIWTPLQVSGGADMEKLEKFGAHTPFGRPGQPAELASIYVQLAAA
                     DASYATGQIYGSSGGAGQP"
     gene            complement(1300..>1589)
                     /locus_tag="VG274_01580"
     CDS             complement(1300..>1589)
                     /locus_tag="VG274_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05367.1"
                     /translation="NAQRRRRTYTAVARLNAFLAVFIANVFSIDLPATRKVGRHALYE
                     EARFGIHPGTGMQTTLRTRNEQHAQGTLLLGFDGRCGLIRRRVWRVRVEFG"
BASE COUNT          355 a          472 c          488 g          274 t
ORIGIN      
        1 cggaccggat gtccgcaaac tttgtgcagt ggcaacggtc gtgcgcgtca ttcgatgcgt
       61 tcaagatcga acggtgtagt aaaaataagt ttcacgtcgg gcggaggaac ttttcgggag
      121 cttcgggaac gcgaagggcc atcgcgcttt tcctcatggg aacaacagga gcccatccgt
      181 gccgcgcatc aaacacaaag accacaatga gttcagcgga cttgccccat acggggacgc
      241 aaaggtcgta acgccgaagg aagactttga tcgcaaccgc gacgggcttt acggtggttt
      301 gcccaaggcg cgtgacaaga acgcggaagg cccggcgccc aacaagccgc atcctgccaa
      361 gaaatagagc gccatttttc cacctctgga atagttcatg cacgctgatt tgaaagaccc
      421 aaccgagaaa cacccgaagc cgccgttcaa aaagcagaag cagccctggc ccggtcttgc
      481 gcgcgatatg gagccgccgc cggaccatgg cgagaagagc tacaaaggtt cgggccgcct
      541 gaaaggccgc aaggcgctga tcactggcgg tgattccggc atggggcgcg cggcagcgat
      601 tgcttacgcg cgcgaaggtg cggatgtcgc gatcaactat ttcccgaccg aagagccgga
      661 cgcgaaggac gtgatcgcgc tgatcgaaga cgctggtcag aaaggtgccg cgatccccgg
      721 cgatctgcgc gacgaggtgt tctgcaaaga actggtcgca aaagccatcg ataagctcgg
      781 cggcctcgac atcgtcgtgt gcaatgccgc gcggcagcaa tcgcacgact ccattctcga
      841 catcacgacc gaggatttcg acgcgacgat gaagacgaac atctatgcgc cattctggat
      901 catcaaagcg gcgctgccgc atctgaagcc tggcgcggtc atcgtcgcga cggcgtcgga
      961 gcaggcctac gatccctccg ccgagcttta cgattacgcg cagaccaagg cggcgacgat
     1021 gaactatgtg aagtcattgg cgaagcaact tgcgtccaaa ggcattcgcg ttaacggcgt
     1081 ggcgccgggt ccgatctgga cgccactgca ggtgagcggc ggcgcagaca tggaaaagct
     1141 cgaaaaattc ggcgcgcaca cgccgttcgg ccgtccgggg caacctgcgg aactcgcatc
     1201 gatctacgtg cagcttgccg ctgccgacgc gagctacgca acgggacaga tttacggctc
     1261 atccggcggc gcgggacagc cttgagcaag gacagaccgt cagccgaact cgacccgcac
     1321 gcgccacacg cgtcgcctga tgagcccgca tcgtccgtcg aagcccagga gcagcgttcc
     1381 ttgcgcgtgt tgctcattgc gggttctcag cgtcgtctgc attcctgtcc cgggatggat
     1441 tccaaagcgc gcttcctcat acagcgcatg gcggccaacc ttccgggtgg ctggcagatc
     1501 gatactgaag acattggcaa tgaacacggc aaggaacgca ttcagccgtg caacggctgt
     1561 gtaagttctg cggcgccgtt gtgcgtttg
//
LOCUS       DASZMC010000083         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1957089,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000083 DASZMC010000000
VERSION     DASZMC010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957089"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..376)
                     /locus_tag="VG274_01585"
                     /pseudo
     CDS             complement(1..376)
                     /locus_tag="VG274_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
     gene            538..2625
                     /gene="glgX"
                     /locus_tag="VG274_01590"
     CDS             538..2625
                     /gene="glgX"
                     /locus_tag="VG274_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWE05368.1"
                     /translation="MESIPDRLEAGLSWPLGATWDGMGANFAVFSTHATRLQLCLFDA
                     AGRREVARLDLPGRSEDVWHGYLPKAQPGLVYGFRAHGPYDPQNGFRFNPQKLLLDPY
                     ARKLVGSLRWTDALFGYRIHSPRADLSIDRRDSAPAMVKAALDDGSFAWGDDKRPCTP
                     WGDTVIYETHVRGLTKRLESVPPRERGTFAGLAHPFVVEHLKRLGVTAVELLPVHAYL
                     QDRRLLEMKLSNYWGYNTLAFFAPEPRYLAGGDAHEMRMAVRRLHAAGIEVILDVVYN
                     HTCEGSELGPTLSWRGLDNASYYRLVAGNRRHLVNDTGTGNTLNVAHPRVLQMVMDSL
                     RHWAQSFHVDGFRFDLGVTLGREERGFDPGAGFFDALRQDPELGRLKMISEPWDLGPG
                     GYQLGHHPPGFAEWNDRFRDCVRRYWRGEPDMRPELARRISGSADVFEHDGRKPWASI
                     NFITSHDGMTLDDVVSFNHKHNEANAENNGDGQPDDHSSGWGAEGLDADAGVKALRRR
                     VKRSMLMTLFGALGTPMLLGGDEFGRSQNGNNNAYCQDNEISWFDWALLRNPDGAELA
                     RFVERLTAIRRRLARPRIFLHGGAEVAAGIADIDWFDERGIRLSEVDWRNVEGRALIL
                     YLARRAPGAPAQISALAMNASVEALDFHLLADVHWRVLLDSAAPAQDETVLEHPLYRI
                     EPHAAALLDGVVA"
     gene            2622..>3082
                     /locus_tag="VG274_01595"
     CDS             2622..>3082
                     /locus_tag="VG274_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05369.1"
                     /translation="MTANDEAVIALAAEAGLDVRWTGADGKSCVVAIDTLASVLRALG
                     LPAGSHAEVRESRTRLRMENRGVPPLIAARAGETVRLPKGGAGEINLASGARRSLRLR
                     DTGDGAVCFCAPSEVGYHRIELDGRDVTLAVAPGHCVRPDDIVAHSRLAGLS"
BASE COUNT          530 a          966 c         1019 g          567 t
ORIGIN      
        1 gtggggaggt cgaagcgcgc tcttcgcgcg cttcgggtgg ggggcgctgc gccgccgcaa
       61 ggatcgactc gacaactcca ttccggtttc tgatcacatc gagattccag aagcgcagga
      121 cgcgatatcc atatgcgctc aagaactttg tgcgctcggc atcgtatcgc gcggatcggg
      181 gatcggcgtg ctgtaagccg tcatgttcga cgatgagttt cgcgctgtga caggcaaaat
      241 cctcgatata ccggccgaac ggcgcctgcc ggcggaagtg gagtccttgc attcacagca
      301 gccgcagcga agaccacagc ttgcgctcag cgtcagtcaa attctgtcgg agcgcgcgtg
      361 cgcgttcgtc agccaatcac tcttctccga ttttggtgta acggcctgca tccccacccg
      421 aaaaacgcga agggcgtttt tcgacctccc cacaaggagg aggttagacg aaactgcgtc
      481 cggaatctcc atcggcgaga tgtttagacg ccttggcgca agcggggcgt ggctctgatg
      541 gaatccattc cggaccggct cgaagcgggg ttgtcctggc cgctgggcgc cacctgggac
      601 ggaatgggcg ctaattttgc ggtgttctcc acgcatgcga cacgtttgca gctctgtctc
      661 ttcgatgcgg cagggcgccg cgaagttgcg cgcctcgatc tgcccgggcg gagcgaggat
      721 gtgtggcacg gctatctgcc gaaggcgcaa ccgggcctcg tctatggatt ccgggcgcac
      781 gggccttacg atccgcagaa cggctttcgc ttcaatccgc aaaagctgct tctcgatcct
      841 tacgcgcgca agcttgtggg cagcctgcgc tggaccgacg cgctgtttgg ataccggatc
      901 cattcgccgc gcgccgactt gagcatcgat cgccgcgaca gcgcgccggc gatggtaaag
      961 gcggcgctcg atgacggcag cttcgcctgg ggcgacgaca agcggccctg cacgccctgg
     1021 ggcgacaccg tcatctatga gacccatgtt cgcggcctga ccaaacgcct tgaatccgtg
     1081 ccgccgcgcg aacgcggaac attcgccggc ctcgcgcatc ccttcgtggt cgaacatctg
     1141 aaacggctgg gggtcacggc cgtggagctg ctgccggtgc acgcctatct gcaggatcgc
     1201 cggctgctgg agatgaagct ctccaactat tggggctaca atacgctcgc cttcttcgcg
     1261 ccggagccgc gctatctcgc cggcggcgac gcgcatgaga tgcgcatggc ggtgcgccgg
     1321 ctgcacgcgg ccggcatcga ggtcatcctt gacgtggttt acaaccatac ctgcgagggc
     1381 agcgaattgg ggcccacgct gtcatggcgc ggcctcgaca atgcgagcta ctatcggctc
     1441 gtcgccggca atcgccgtca cctggtcaac gacaccggca cggggaatac gctcaacgtc
     1501 gcccatccgc gcgtgttgca gatggtgatg gattcgctgc gccactgggc acagtctttc
     1561 cacgtcgacg gcttccgctt cgatcttggc gtcacgctcg gacgggaaga gcgaggcttc
     1621 gatcccggcg cggggttttt cgacgccttg cgccaggatc ccgaacttgg ccggcttaag
     1681 atgatctcgg aaccttggga tttgggaccg ggaggctacc agctcggaca tcatccgcca
     1741 ggcttcgcag aatggaacga ccgcttccgc gattgcgtcc gccgctattg gcgcggcgaa
     1801 cccgacatgc gtcccgaact ggcgcggcgc atttccggct ccgcggatgt gttcgagcat
     1861 gacggaagaa aaccctgggc ttcgatcaac ttcatcacca gccatgacgg catgacgctc
     1921 gacgatgtcg tgagcttcaa tcacaagcac aacgaagcaa acgccgagaa caatggcgac
     1981 gggcagccgg acgatcattc gtcgggctgg ggcgcggaag gcctggacgc ggacgctggc
     2041 gtgaaagcgc tgcgcagacg cgtcaagcgc tccatgctga tgacattgtt cggcgctctc
     2101 ggcacgccga tgctgctcgg cggcgacgaa tttggccgct cgcagaacgg caacaacaac
     2161 gcctattgcc aggacaacga aatctcatgg ttcgactggg cgctgttgcg caaccccgac
     2221 ggcgcagagc ttgcgcgttt cgtcgagcgc ctgaccgcga ttcggcgcag gcttgcgcgg
     2281 ccgcggattt tcctccatgg cggcgcagag gtcgccgcgg gcattgccga catcgactgg
     2341 ttcgacgaac ggggcatacg gctttcggaa gttgattggc gcaacgtcga aggccgcgcg
     2401 ctgatcctgt atctggcgag gcgggcgccg ggcgcaccgg cgcagatcag cgcgttggcg
     2461 atgaatgcct ccgttgaggc tctcgacttt cacctccttg ccgatgtgca ctggcgcgtg
     2521 ctgctcgaca gcgccgcgcc cgcgcaggat gaaacagtgc tggaacatcc gctctatcgc
     2581 atcgagccgc acgccgcggc gctgctcgac ggcgtcgtgg cgtgacggca aacgacgaag
     2641 ccgtcatcgc gctcgcggcg gaagccggac tggacgtgcg atggaccggc gcagatggaa
     2701 agagctgcgt cgttgccatc gatactctcg cgtccgtctt gcgggcgctt ggcctgccgg
     2761 ccggttcgca cgccgaggtc agggaaagcc gtacccgcct gcgaatggag aaccgggggg
     2821 tcccgccgct gattgccgct cgggcgggcg agactgtgcg tttgccgaag ggtggcgccg
     2881 gcgaaatcaa tctcgcgagc ggtgcgcgta ggagtttgcg actgcgcgac acgggcgatg
     2941 gcgctgtctg tttctgcgcg cccagcgaag tgggctatca ccggatcgag ctcgacggcc
     3001 gggatgtcac tctggcggtg gcgcccgggc attgcgtgcg tcccgacgac atcgttgcgc
     3061 atagccggct cgccggcctg tc
//
LOCUS       DASZMC010000084         6840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3889254,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000084 DASZMC010000000
VERSION     DASZMC010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3889254"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /gene="rseP"
                     /locus_tag="VG274_01600"
     CDS             complement(<1..682)
                     /gene="rseP"
                     /locus_tag="VG274_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HWE05370.1"
                     /translation="MLGTVQDLIGGIGYYVVPFLLMITPIVFFHELGHFLVARACGVR
                     IETFSIGFGPEIFGWHDRAGTRWKVAWIPLGGYVKFFGDTNAASMPDRETVASMTAKE
                     RIEAFPLKPLYQRALIVAAGPAANFVLAIVILAGFLMALGTYVAAPIVSTVTPASAAA
                     QAGFRPGDTIISINGAPIESFDQIRGFVWDRAGEKLNVEVRRDGKTLLLPVTPTLTKV
                     ELAGGAQKV"
     gene            complement(725..1948)
                     /gene="dxr"
                     /locus_tag="VG274_01605"
     CDS             complement(725..1948)
                     /gene="dxr"
                     /locus_tag="VG274_01605"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWE05371.1"
                     /translation="MSEDPDVTFLAGALERRLTILGSTGSIGVSTLDVVAHVRARYGA
                     KSFPIEALTAHSNVEVLAEQARRFRPRLAVVSDERRYADLRQALAGEGIETAAGPASV
                     IEAAGRASDVVMVAIVGAAALEPTLAAARRGATIALANKECVVAAGDLFRDALDASRA
                     TVIPVDSEHNAAFQLLDFAQSHAFERLTLTASGGPFREWPRERMQVATPEQAVAHPNW
                     SMGAKISVDSATLMNKGLELIEAHVLFALSADKLGVIVHPQSVVHCLVSYCDGSTLAH
                     LSAPDMRTPIAHALGWPTRISSPSLRLDLAGVQHLTFEAPDLERFPCLRLAQDCLHAG
                     GLTPTILNAANEIAVAAFLGREIGFLDIAGIIEDTLASRCHDVAECPSDLAGILAVDA
                     EARDIAHRFCRARAA"
     gene            complement(1945..2841)
                     /locus_tag="VG274_01610"
     CDS             complement(1945..2841)
                     /locus_tag="VG274_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWE05372.1"
                     /translation="MKHSSPIARLAPLQANASRSAGEPDANNFQWREWLARASFGALL
                     ASLAIAATFAGGFTFAIFIAVVAFAAAREWHRMVSGPRYAAAIAVTAIAIAASLGWAV
                     AARDSQWPVWLLLLGSVLAAMCAFATGTRPLSNGLGTLYVGLPALSLVALRIHAPQGA
                     AAVLVLFIAVWAADTGALVWGRFLGGPKLVPALSPNKTWTGFIAGTIFASAAVTFCVK
                     LLGGSLWQAALLGLALALAGHCGDLFESWVKRRAGRKNSGGLIPGHGGVLDRVDSTLF
                     AAPLAALLVFVFGLDLLCGGHP"
     gene            complement(2838..3602)
                     /locus_tag="VG274_01615"
     CDS             complement(2838..3602)
                     /locus_tag="VG274_01615"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HWE05373.1"
                     /translation="MQPKKITAMNGRAPSMESLPRHVAIIMDGNGRWARRRHLPRAAG
                     HVAGVGAVRSVVRASIDTGLSYLTLYAFSSENWKRPPTEVTHLMCLFRAYFREDVDEL
                     VERNVRMRIIGSRQRAESDILAMIEDGERRTAHNTGLNLTFAFDYGAQEEIVAAAREM
                     AQAAVLGELDPDAITPELFSTRLLTHGLPNPDLLIRTSGEHRLSNFLLWQSAYAELAF
                     VDTLWPDFTGAHLFEALQVFAQRERRFGAVTPEAVA"
     gene            complement(3602..4162)
                     /gene="frr"
                     /locus_tag="VG274_01620"
     CDS             complement(3602..4162)
                     /gene="frr"
                     /locus_tag="VG274_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWE05374.1"
                     /translation="MSGTFDKEDLNRRMNGSITALKGEFAGLRTGRANPALLDPVTVE
                     AYGNPMPVTQLGTVSAPEPRLLTVQVWDKSMVKSVDKAIRDAGLGLNPQMDGQLLRIP
                     IPELNEERRRELAKLAHKYAEQARVAVRNVRRDGMEILKRLEKDHKISQDEHHGLGEE
                     LQKVTDAHIRDIDHALHAKEQEIMQV"
     gene            complement(4190..4915)
                     /gene="pyrH"
                     /locus_tag="VG274_01625"
     CDS             complement(4190..4915)
                     /gene="pyrH"
                     /locus_tag="VG274_01625"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWE05375.1"
                     /translation="MGEAPKYGRVLLKVSGEALMGAQTFGIDIGTVDRIAADVKEAAQ
                     GGVQVCLVIGGGNIFRGLAGAADGIERATADYMGMLATVMNALALQAALERIDVPTRV
                     QSAIPMESVCEPYIRRRAIRHMEKGRVVIFAAGTGNPFFTTDTAAALRAAEMNCAAML
                     KGTQVDGVYSADPKRDPKAERFEKLSYHQVLALDLQVMDASAISLSRENRIPIIVFSI
                     HDRGALAAVLKGKGRCTIISDEG"
     gene            complement(4937..5863)
                     /gene="tsf"
                     /locus_tag="VG274_01630"
     CDS             complement(4937..5863)
                     /gene="tsf"
                     /locus_tag="VG274_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWE05376.1"
                     /translation="MTNVTAGMVKELRDKTGAGMMDCKAALAETGGELESAVDWLRKK
                     GLAKAAKKAGRVAAEGLVGVAVAGGSGAIVEINSETDFVARNETFKAFVKEAAQLALE
                     TGGDVAKLLASKMSSGSTVQVSVTELIAKIGENISVRRTAVLSVSPGVVAAYVHNAVA
                     PESGKMGVLVALRSTADPAKLATLGKQLAMHIAAAAPLALTSGDLAEDVVARERDVQT
                     ELARQSGKPENVIAKMVEGRMRKFYEESVLLSQAFVIDGETPVAKVVENAAGEFGAPV
                     EIAGFVRFAVGEGVDKPDAGDFASEVKAMAGH"
     gene            complement(5879..6664)
                     /gene="rpsB"
                     /locus_tag="VG274_01635"
     CDS             complement(5879..6664)
                     /gene="rpsB"
                     /locus_tag="VG274_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWE05377.1"
                     /translation="MALPEFSMRQLLESGAHFGHRTQRWNPKMAPYIYGSRNSIHIMD
                     LTQTVPLLHQALVALRDVVTGGGRVLFVGTKRQASEPIAAAAKRCAQYYVNHRWLGGT
                     LTNWQTISNSIKRLRGIEETLTSAPGSGLTKKELLGLLRERDKLGRSLGGIKEMGGLP
                     NMLFVIDTNKEQIAVAEAKKLGIPVAAILDSNSDPDGIAYPVPGNDDAARALSLYCDL
                     VARAIIDGLSEGQVAAGVDVGEAEDVHEPRILSDAPVEAPATA"
BASE COUNT         1245 a         2258 c         2084 g         1253 t
ORIGIN      
        1 cgaccttctg cgcgccgccc gccaactcga ccttggtcag cgtcggcgtg accggcaata
       61 ggagagtctt gccgtcccgc ctcacctcga cgttgagttt ttccccggcg cgatcccaga
      121 cgaagcctcg aatctggtcg aaactttcga tcggtgcgcc gttgatggat atgatcgtat
      181 cgcctggtcg aaagcccgcc tgcgcagccg cgctcgcggg cgtcaccgtg cttacgatcg
      241 gcgccgccac atatgtgccg agcgccatca gaaaaccggc caggatcacg atcgccagca
      301 cgaaattcgc cgccggcccg gcggcaacaa tcagcgcgcg ctggtagagc ggcttgagcg
      361 gaaacgcctc gattcgttcc ttcgccgtca tcgacgccac agtttcgcga tcaggcatgc
      421 tcgccgcgtt ggtgtcgccg aagaatttca cgtagccgcc aagcggaatc cacgcgactt
      481 tccagcgcgt gcctgcgcgg tcatgccagc cgaagatttc cgggccgaag ccgatggaga
      541 acgtctcgat gcgcacaccg catgcgcgag cgacaagaaa gtgaccaagt tcgtgaaaga
      601 agacgatcgg ggtgatcatc agaagaaacg gcactacata atagccgatt ccgccaatca
      661 ggtcctgcac ggttcccagc atctcatacc cttcttcccc cactccagca tattcatatt
      721 cgtctcaggc ggcgcgcgcc cggcagaacc gatgcgcgat gtccctcgct tcggcatcga
      781 cagcaagtat gcccgcgaga tcggacggac attcggccac gtcgtgacac cggctcgcca
      841 gcgtgtcttc tatgatgccg gcaatgtcca gaaaaccgat ctctcttccg agaaaggccg
      901 caacggcgat ctcgttggcg gcgttcagga tcgtaggtgt cagtcctccg gcatgcaagc
      961 agtcctgggc gagcctcaga caggggaatc gttccaggtc cggtgcttcg aaggtcagat
     1021 gctgcacgcc ggcaaggtcc agccgcaatg agggcgatga aatgcgcgtc ggccaaccga
     1081 gggcgtgcgc aatcggcgtg cgcatgtcgg gcgcggaaag atgcgccagg gtggatccgt
     1141 cgcaatagga gacgagacag tgcacgacgg actgcgggtg gacgatcaca ccgagtttgt
     1201 ccgcgctcag cgcaaagaga acatgcgctt cgatcagctc gaggcctttg ttcatcagcg
     1261 ttgcgctgtc gaccgagatc ttggctccca tcgaccagtt cgggtgcgcg accgcctgtt
     1321 caggcgtcgc gacctgcata cgttcgcgcg gccactcgcg aaacggtccg cccgatgccg
     1381 tgagcgtgag gcgctcgaaa gcgtgggatt gcgcgaagtc gagcaattga aacgcggcgt
     1441 tgtgctcgga atccacaggg atgaccgtcg cgcgcgacgc gtcgagcgcg tcacgaaaaa
     1501 gatctccggc ggcgacgacg cattccttgt tggcgagcgc gattgttgct ccgcggcgtg
     1561 ccgccgcaag cgttggttcg agagcggcgg cgccgacgat cgcgaccatc acgacgtcgc
     1621 tggcccggcc tgccgcctcg atcaccgacg cagggccggc ggcagtttcg atgccttctc
     1681 ccgcgagcgc ctggcgaagg tccgcgtaac gccgctcgtc ggagacgacg gccaggcgcg
     1741 gccggaaccg gcgggcctgt tcagccagca cttcaacgtt ggaatgggca gtcagggcct
     1801 cgatcggaaa tgatttcgcg ccgtaacgcg cccgcacatg cgccacgaca tcgagcgtgc
     1861 tcacgccgat cgaaccggtg ctgccgagaa tcgtcagccg ccgctcgagt gcgccggcca
     1921 ggaatgttac atctgggtct tcgctcacgg atggcctcca cagagcagat ccagaccgaa
     1981 cacgaacacc aggagcgcgg caagcggcgc cgcaaaaagc gtcgaatcca cacgatcgag
     2041 gacaccgcca tgtccgggaa tgagtccgcc gctgttcttc cgtcccgccc gccgcttcac
     2101 ccaggattcg aaaagatcgc cgcaatgtcc ggcgagcgcg agggcgaggc cgagcagcgc
     2161 cgcctgccat aggcttcccc ccaacagctt cacgcagaag gtaactgccg ccgacgcgaa
     2221 aattgtgccc gcgatgaatc cggtccatgt cttgttcgga gagagtgcag gaacaagctt
     2281 cggtccgcca agaaaccggc cccagacgag cgcacccgta tccgcggccc acactgcgat
     2341 gaaaagcacg agcactgcgg cagcgccctg tggcgcatga atacgaagtg caacaagcga
     2401 tagcgcgggc agaccgacat agagcgtgcc caatccgttc gagagcggcc gcgtgccggt
     2461 tgcgaacgcg cacattgccg ccagcacgct gccgagcaaa agcagccata ccggccattg
     2521 cgagtcccgc gccgcgacag cccagccgag acttgcggcg attgcgattg cagtaaccgc
     2581 gatggcggct gcataacgcg gtccgctgac catgcgatgc cattcccgcg ctgcggcgaa
     2641 tgcgacaacg gcaatgaaga tcgcaaacgt gaatccgccc gcgaacgtgg cggcgatggc
     2701 aagactcgcc agcagcgctc cgaacgacgc acgcgccagc cattcccgcc attggaaatt
     2761 gtttgcgtcc ggctcacccg ccgatcggga agcgttcgcc tgcagaggcg caagcctcgc
     2821 gattggagag gaatgcttca agccacggct tccggagtta cggcgccgaa gcgccgctcg
     2881 cgttgagcaa acacctgcaa agcctcgaac agatgggcgc ccgtgaaatc gggccagagc
     2941 gtgtcgacga acgccagctc cgcataggcc gactgccaga ggagaaaatt gctgagccgg
     3001 tgctcgccgc ttgtgcgaat tagcaggtcg gggttcggca ggccgtgagt aaggagtctg
     3061 gtgctgaaga gctccggcgt gatcgcgtcg ggatcgagtt caccgagcac tgcggcctgc
     3121 gccatttccc gggctgcggc gacgatttct tcctgcgctc cataatcgaa cgcaaaggtc
     3181 agattgagac cggtgttgtg cgccgtccgc ctctcgccgt cttcgatcat ggcgagaata
     3241 tcgctctccg cccgctgccg gctgccgatg atccgcatac ggacgttccg ctccacgagc
     3301 tcatcgacgt cttcccggaa atacgcccgg aacaggcaca tcaaatgggt cacttccgtt
     3361 ggcggcctct tccagttctc tgaagagaac gcgtagagcg taagatagga caagcctgta
     3421 tcgatcgagg cgcgcaccac cgagcggacg gctcccacgc ccgcaacgtg accggcggcg
     3481 cgcggcagat gccggcgccg cgcccagcgt ccgtttccgt ccatgatgat cgcgacgtga
     3541 cgcggcaggc tctccattga gggcgcccgt ccattcatcg ccgtgatctt cttcggttgc
     3601 atcagacctg catgatctcc tgctccttgg cgtggagcgc atgatcgatg tcacggatat
     3661 gcgcatccgt gaccttctgc aattcctcgc cgagtccgtg atgctcgtcc tggctgatct
     3721 tgtgatcctt ctcgaggcgc ttgagaattt ccatgccgtc gcggcgcacg ttgcgcacgg
     3781 cgacccgcgc ctgctcggcg tatttgtgcg cgagcttggc aagttcacgg cggcgctcct
     3841 cgttgagttc gggaatgggt atgcgcagga gctggccgtc catttgcgga ttcagaccga
     3901 gccccgcgtc ccgtatcgcc ttgtccacag acttgaccat ggacttgtcc cacacctgca
     3961 cagtgagcag tcgcggctcg ggcgcactca ccgtgccaag ctgcgtgacg ggcatggggt
     4021 tgccatatgc ctcgaccgtt acgggatcga gcaaggcggg gttcgctctg ccggtgcgca
     4081 ggccggcgaa ctctcccttc aatgcggtga tcgagccgtt catgcggcgg ttcagatctt
     4141 ccttgtcgaa cgtgcctgac atttgcggat cctccgtgat tcccggagct tagccctcat
     4201 cggaaatgat tgtgcagcgt cctttgccct ttagaacggc agcaagcgct ccgcgatcat
     4261 ggatcgaaaa gacgatgatg gggatacgat tctcccgcga gaggctgatc gcagatgcat
     4321 ccatcacttg aagatcgagg gctaacactt ggtgatagct aagtttttcg aatctttccg
     4381 ctttcggatc gcgcttcggg tcggcgctgt agacgccgtc aacctgcgtt cccttgagca
     4441 tcgccgcgca gttcatctcg gccgcccgca gggcggccgc cgtatcggtg gtgaagaacg
     4501 gattgccggt gcccgccgca aaaatgacca cccgcccctt ctccatgtgc cggatcgcgc
     4561 gccggcgaat atagggctcg caaaccgatt ccatcggtat cgccgactgg actcgcgtcg
     4621 gcacgtcgat gcgctccagg gcagcctgca gcgcgagcgc gttcatcacc gtcgccagca
     4681 ttcccatgta atcggcggtc gcgcgctcga ttccatcggc agcgccggcg aggccccgga
     4741 agatgttgcc gccgccgatc acgagacaga cttgcactcc cccctgcgcg gcctctttca
     4801 cgtcggcggc aattcgatcc acggtcccga tgtcgatgcc gaaggtctgg gcgcccatca
     4861 acgcttcgcc cgagaccttg agcaggaccc ggccgtattt cggcgcttcg cccatcgcag
     4921 tttcccccgc ccggtttcag tggccggcca tcgccttcac ttcgctggcg aaatcgccag
     4981 cgtccggctt gtcgacgccc tcgccgacgg caaagcgcac gaagcccgcg atctcgaccg
     5041 gggcgccaaa ctctccggcg gcattttcga ccaccttggc gaccggcgtt tcgccgtcga
     5101 tcacgaacgc ctgcgagagc agcacggatt cttcgtagaa tttccgcatg cgtccctcga
     5161 ccattttcgc gatgacgttt tccggctttc cggattgacg cgcaagctcc gtttgcacgt
     5221 cgcgttcgcg cgcgaccaca tcttccgcca aatcgcccga cgtcagggca agcggcgccg
     5281 ccgccgcaat atgcatcgcg agctgcttgc cgagtgttgc gagcttcgcc gggtctgccg
     5341 tcgatcgcaa cgccaccagg actcccatct ttcccgattc cggcgccacc gcgttgtgga
     5401 cgtaggcagc gaccacgccg ggcgagaccg aaagcaccgc tgtccgccgc acgctgatgt
     5461 tctcgccgat cttggcgatc aattcggtga cgctcacctg caccgtcgag cccgacgaca
     5521 ttttcgaggc gagcagcttt gccacgtcgc cgcccgtctc gagcgcgagc tgagccgctt
     5581 ccttcacgaa ggctttgaag gtttcgttgc gcgcgacgaa atcggtttcc gaattgattt
     5641 cgacgatcgc gcccgaaccg ccggcgactg caacgccaac caggccttcc gccgcgacac
     5701 ggcccgcctt cttcgcggcc tttgccagac ccttcttgcg cagccagtcg actgccgatt
     5761 cgagctcgcc gccggtctcc gcgagcgccg ccttgcaatc catcatcccc gcgcccgtct
     5821 tgtcgcgcag ctccttcacc attcccgcgg tgacgttcgt catgacattc ctttgtgctt
     5881 aagcggtggc tggagcttcg accggcgcgt cggagagaat gcgcggctca tgcacgtcct
     5941 cggcttcgcc gacgtccacg ccggccgcga cctggccttc cgacaatccg tcgatgatcg
     6001 cccgcgccac gaggtcgcaa taaagcgaga gcgcgcgcgc cgcgtcgtca ttgcccggaa
     6061 cgggataggc gatgccgtcc ggatcggagt tcgaatccag aatggcagcg accggaatgc
     6121 cgagcttttt ggcttcggca accgcgatct gttccttgtt ggtgtcgatt acgaacagca
     6181 tgttgggcag gccgcccatc tccttgatgc cgccgagcga ccgcccgagc ttgtcgcgct
     6241 cgcgcaacag accgagaagt tccttcttgg tcagaccgga gcccggcgcg gaagtgagcg
     6301 tctcctcgat gccgcgcagc cgcttgatgg aattcgagat cgtctgccag ttggtgagtg
     6361 tgccgccgag ccagcggtga ttgacatagt attgcgcgca gcgtttggcc gcagcggcga
     6421 tcggttcgct cgcctggcgc ttggtgccga cgaacagcac ccggcccccg cccgtcacca
     6481 cgtcgcgcag cgccaccagc gcctgatgca gcagcggaac ggtctgcgtc aggtccatga
     6541 tgtggatgga attgcgcgag ccgtagatgt agggcgccat cttcggattc catctctgcg
     6601 tgcgatggcc gaaatgcgcg cccgactcca gaagctggcg catggaaaat tctggcaatg
     6661 ccatgggaaa acgtctcctt ttccggttta gcctgacgcg aggggaccac cccgaagggc
     6721 aaccggacgg ggttgaggca acttgcccca ctccccaccc tgcgcgtgcg gaatggcagg
     6781 ggttatagga acttggcttg acgggcgcaa gcgctcccgg gtggagtgcc gccatgctgc
//
LOCUS       DASZMC010000085         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4177507,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000085 DASZMC010000000
VERSION     DASZMC010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4177507"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /gene="holA"
                     /locus_tag="VG274_01640"
     CDS             complement(<1..964)
                     /gene="holA"
                     /locus_tag="VG274_01640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWE05378.1"
                     /translation="MLIRAAESERIIARFPAELAAALLFGPDQGLVRERAERLTKTIV
                     PDLRDPFRIAEIDGARLLEDPAWLEAEASAISMTGGRRVVRIRGAGNALGPVFDSFLA
                     RSTSDVLVVVEGGDLAKNSSLRETFEEASNAAAIPCYLDSTEAIADLLRVSLKADSIT
                     IAPDALDEAVSLLGADRGTTRREVEKLILYAHGHARVTLEDIRAIMGDDAEARIEEAC
                     DAAGDGDTHRLDLALERLWSAGVTPIAILRVGLGHFQRLALARAQIEKGESQEAVARR
                     ARPPVHFSRLGSFRAQLRSWSSEKLGEVLDLLLETEALCKTTAVP"
     gene            complement(965..1483)
                     /gene="lptE"
                     /locus_tag="VG274_01645"
     CDS             complement(965..1483)
                     /gene="lptE"
                     /locus_tag="VG274_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWE05379.1"
                     /translation="MSGRVFEALFAVALACCTAGCSFHPLYARAGPGAGGQQVFNSIY
                     VEPVDGERAGYELRNSLIDLLEGAERPQLALYRLKVTVKQTIEGTAVQTNGSITRYSY
                     TMVANYELSDIHSGKDVTKGTESTVSGYDVVASPYATLVAQQDAQKLGARDIADRIRI
                     DLGVYFSKRSSS"
     gene            complement(1480..>2429)
                     /locus_tag="VG274_01650"
     CDS             complement(1480..>2429)
                     /locus_tag="VG274_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWE05380.1"
                     /translation="HAILHLLYARFFTRGLKQLGLADVDEPFAGLFTQGMVCHETYRD
                     RAGQWISPEEVEKRDGGVRRKDNGEMVVVGPSEKMSKSKKNVVAPEPIIDVYGADTIR
                     WFMLSDTPPERDVEWTETGAEGCWRFVQRVYRLVTDIGGLPAIGAGIPAALAEPSMQL
                     RQATHRAVAWVTEDLDALRFNRAVARIYELANAISGAPADVLGAVKREALEILVRLTA
                     PMMPHLAESCWEALGHSSMLIDEPWPKADPALVRADTITIAVQVDGKRRGEIAIDRGA
                     NDETVKAAALALDGVERALAGKSPRRIIHVPERIVNIVT"
BASE COUNT          434 a          803 c          742 g          450 t
ORIGIN      
        1 caggcacggc cgtggttttg caaagcgcct ccgtctccag gagaagatcg aggacctccc
       61 ccaatttctc actgctccag cttcgcagct gcgcccggaa ggaaccaagc cgcgagaaat
      121 gcaccggcgg tctggcacgg cgcgcaacag cttcctgact ctcgcctttt tcgatttgcg
      181 ccctggccag cgcgagccgc tgaaaatgtc cgagcccgac gcgcagaata gcgatcggcg
      241 tgacgccggc gctccatagc ctctcgagcg ccaggtcgag gcggtgcgta tcaccgtctc
      301 ccgctgcatc gcaggcctcc tcgatacggg cctccgcatc gtcgcccatg atcgcgcgga
      361 tgtcttccag cgtcacgcgt gcatggccgt gcgcgtagag gataagcttc tcgacctccc
      421 ggcgggtggt gccgcgatcg gcgccgagaa gcgaaacggc ctcgtcgaga gcatccggcg
      481 cgatggtgat cgaatccgcc ttcagcgaga cccgcagaag atccgcaatc gcctcggtgg
      541 aatcgagata gcagggtatt gccgcggcat tggaggcttc ctcgaatgtt tcgcgcagac
      601 tggaattctt cgcgagatcg cccccttcga ccacgacgag cacatccgac gtcgagcggg
      661 ccagaaatga atcgaacacg ggtccaagcg cgttgccggc gccgcggatt cgcaccacgc
      721 gacgcccccc ggtcatcgaa atcgccgacg cctccgcctc cagccatgcc gggtcctcga
      781 gaagccgcgc tccgtcgatc tccgcaatcc ggaatggatc gcgtaggtcc ggtacgatgg
      841 tcttcgtcag acgctctgca cgctcgcgca ccaatccctg atcgggacca aacagcagcg
      901 cagctgcgag ctccgcggga aagcgggcaa tgatgcgttc gctttcggcg gccctgatga
      961 gcattcagct gctggagcgc ttggaaaagt agacaccgag atcgatgcgg atgcggtctg
     1021 cgatgtcgcg cgcccccagc ttctgcgcgt cctgctgtgc gacgagagtg gcgtagggcg
     1081 aagccaccac gtcgtagcca gaaacggtcg attcggttcc tttggtcacg tccttgcccg
     1141 aatgaatgtc gctgagttcg tagttggcga ccatggtgta gctgtaacgg gtgatcgagc
     1201 cattggtctg cacggccgtg ccttcgatgg tctgcttcac cgtcactttc aggcgataca
     1261 gggcaagctg cggacgttcc gcgccttcca gcagatcgat cagcgagttt ctgagttcgt
     1321 atccggcgcg ttccccatcg accggctcga catagatcga attgaacacc tgttgtccgc
     1381 cagcgcccgg tccggcgcgc gcataaagcg gatgaaagct gcatcccgcc gtgcagcacg
     1441 caagcgccac ggcgaacaaa gcctcgaaga cccgtccgct catgtgacga tattcacgat
     1501 gcgctcgggc acatggatga tccggcgcgg agattttccg gccaatgccc gttcgacgcc
     1561 gtccagagcc agcgccgcgg ccttgacggt ctcgtcgttg gcgccccggt cgatggcgat
     1621 ctcgccccgc cgctttccgt ccacctgcac cgcgatcgtg atggtatccg cccgcacgag
     1681 cgccggatcg gccttcggcc acggttcgtc gatgagcatc gaggaatgcc cgagcgcttc
     1741 ccagcacgat tcggccagat ggggcatcat gggcgcggtc aggcgcacca gtatttcgag
     1801 agcttcccgt ttcaccgcgc cgagcacgtc cgcgggagcg ccgcttatcg cattggcaag
     1861 ctcgtagatg cgcgcgacgg cacggttgaa tcgaagcgca tcgagatctt cggtcaccca
     1921 tgcaacggcg cgatgcgtgg cctggcgcag ctgcattgac ggctctgcga gggccgccgg
     1981 gatgcccgct ccaattgctg gaaggccgcc gatgtcggtg acgagccgat acacgcgctg
     2041 aacgaagcgc cagcagcctt cggcgcccgt ttccgtccat tcgacgtcgc gctcgggagg
     2101 cgtgtcggac aacatgaacc agcggatcgt gtccgcgccg taaacatcga tgatcggttc
     2161 gggcgcaacg acgtttttct tcgacttcga cattttttcc gacggtccga ccacgaccat
     2221 ctcgccattg tctttgcgcc ggacgccgcc gtcgcgcttc tccacttctt ccggcgaaat
     2281 ccactggccc gcgcggtcgc ggtaggtctc atggcacacc atgccctgcg tgaagaggcc
     2341 cgcaaatggc tcgtcgacat ctgcaagtcc gagctgtttc agcccgcggg tgaagaaccg
     2401 cgcgtagagc agatgcaaaa tcgcgtgct
//
LOCUS       DASZMC010000086         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5255667,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000086 DASZMC010000000
VERSION     DASZMC010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5255667"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1458
                     /gene="glmM"
                     /locus_tag="VG274_01655"
     CDS             106..1458
                     /gene="glmM"
                     /locus_tag="VG274_01655"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWE05381.1"
                     /translation="MANSRKLFGTDGVRGLANKFPMTPEVAMKVGMAAGRIFTRGEHR
                     HRVVIGKDTRLSGYMIESALVAGFTSVGMDVFQFGPLPTPAVAMLTRSLRADMGVMIS
                     ASHNAFHDNGIKLFGPDGEKLSDEREQEIEALMQNGLEEGLTSAETIGRAKRVDDSQA
                     RYIEFAKRTFPRNLRLDGLRIVLDCANGAAYKVAPAVLWELGADVISLGVDPNGMNIN
                     LDCGSTSPEAMQNKVRDVRADFGIALDGDADRVVMTDEQGRIIDGDQILALIARSWSK
                     NGLLKGGGVVGTVMSNAGLDRYLQSQGLKLARASVGDRYVIAEMKKGGFNVGGEQSGH
                     IVLSDFATTGDGLVAALQVLAVLAMDGVKASEAAHLYEPLPQVMENVRFKKGMPLEDA
                     RVKKCIESGTERLNGSGRILVRKSGTEPVIRVMAEGDDEKLVRKIVREIAASIEEAAA
                     "
     gene            1455..2240
                     /gene="thiD"
                     /locus_tag="VG274_01660"
     CDS             1455..2240
                     /gene="thiD"
                     /locus_tag="VG274_01660"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWE05382.1"
                     /translation="MTPARVLIIAGSDSSAGAGIQADLKTAQAFGVYAQTAITAVTAQ
                     NTRGVRAVHLVPPEIVAAQIEACLSDIGADAIKIGMLSSAKVVKAVAAALAKRARKIP
                     IVLDPVMVSTSGHSLLATNAVAAMTKELFPLATLVTPNIPEAKALAHLKGTKRNDAEA
                     AARKLIAMGAGAALIKGGHATRSTIDDVMVWKGGVEVYAFPRIKTKHTHGTGCTLATA
                     IACGLAQGLSLPLAAGRAREYVQNAIESAPGLGKGRGPLNHDI"
     gene            complement(2274..>2712)
                     /locus_tag="VG274_01665"
     CDS             complement(2274..>2712)
                     /locus_tag="VG274_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05383.1"
                     /translation="CALLRQLVEVEYLAWAFETKDAESARWLRSSREDRQSFFTPAKL
                     RKAAEGRFRSVDYSYHCELGGHPVPGSWRLLNDDTAIAQLMLSDCLGHSGRVWDHIVG
                     WSKGYLLSDIVQEKVTIMLDKYRNWKKLDLLTVLPPPPSDFDG"
BASE COUNT          553 a          812 c          853 g          494 t
ORIGIN      
        1 caacaaggcg taatccagcg tgattcgagg gctggcgctt ttcgcctaag gttttgttat
       61 gattggggat agaggataga aatcccgccc tatatcgagg ttcccatggc caattcccgc
      121 aagttgttcg gcaccgatgg agtgcgcggg ctcgccaaca aattcccaat gacgccggaa
      181 gtcgcgatga aggtcggcat ggctgccggc cgcatcttca cgcgcggcga acaccgccat
      241 cgcgtcgtga tcggcaagga cacgcgtctg tccggataca tgatcgagag cgcgctggtc
      301 gcggggttca cgtcggtcgg catggatgtg ttccagttcg gcccgctgcc gacgccggcg
      361 gtggcgatgc tgacgcgctc gctgcgcgcc gacatgggtg tgatgatctc cgcgtcgcac
      421 aacgcgttcc acgacaacgg catcaagctg ttcggcccgg acggcgagaa gctctccgat
      481 gagcgcgagc aggaaatcga agcgctgatg cagaacggcc ttgaagaagg cctgaccagc
      541 gcggagacca tcggccgtgc caagcgcgtg gacgactctc aagcccgcta catcgaattc
      601 gccaagcgca ccttcccgcg caatcttcga cttgacgggc tgcgcatcgt gctcgattgc
      661 gcgaacggcg ccgcctacaa ggttgcgcct gccgtgttgt gggaacttgg cgctgacgtc
      721 atctctcttg gcgtcgatcc aaacggcatg aacatcaatc tcgattgcgg ctcgacctcg
      781 ccggaagcga tgcagaacaa ggtgcgcgac gtgcgcgcgg atttcggcat cgcgctcgac
      841 ggcgatgcgg atcgcgtggt catgacggac gagcagggcc gcatcatcga cggcgatcag
      901 attttggcgc tgatcgcgcg ctcgtggtcg aagaacggtt tgctcaaggg cggcggcgtg
      961 gtcggcacgg tgatgtcgaa cgccggcctt gaccgctatc tgcaatcgca gggcctgaag
     1021 cttgcgcgcg cgtccgtagg cgatcgctat gtcatcgcgg agatgaagaa gggcggtttc
     1081 aacgtcggtg gcgaacagtc ggggcacatt gtgctctccg atttcgcgac gaccggtgac
     1141 ggcttggtcg cggcgctgca ggtgctggct gtgctggcga tggacggcgt gaaggcgagc
     1201 gaggccgccc atctttatga gccgctgccg caggtgatgg agaatgtccg cttcaagaag
     1261 ggcatgccgc tcgaagatgc gcgcgtgaag aagtgcatcg agtcgggaac ggagcgcctt
     1321 aacggctccg gccgcatcct tgtgcgcaaa tccggcaccg agcccgtcat ccgcgtgatg
     1381 gcggaaggcg acgacgagaa gctggtgcgc aagatcgtgc gcgagatcgc ggcttcgatc
     1441 gaggaagccg cggcgtgacg ccagcccgcg ttctcatcat cgcgggatcg gactcaagcg
     1501 caggcgccgg gatccaggcc gatctcaaga ccgcgcaggc gttcggcgtc tacgcgcaga
     1561 ccgcgatcac cgcggtgacg gcgcagaaca cgcgcggcgt gcgcgctgtg catctcgttc
     1621 cgccggagat cgttgcagca cagatcgaag cgtgcctgtc cgacatcggc gccgacgcga
     1681 ttaagatcgg gatgctcagc tcggcgaaag tggtgaaagc cgtcgccgcc gcgctggcga
     1741 agcgcgcccg caaaatccca atcgtgctcg atccagtcat ggtctcgacc agcggccaca
     1801 gcctgctcgc aacgaacgct gtggcggcga tgacgaaaga gttattcccg ctcgcgacac
     1861 tggtcacgcc gaacatcccg gaagcgaaag cgctcgcgca tctgaaaggc accaaacgca
     1921 acgacgccga agccgccgcg cgcaagctga tcgcgatggg tgcaggggcc gcactcatca
     1981 aaggcggcca cgcaacgcgc tcgaccatcg acgacgtgat ggtgtggaag ggcggcgtgg
     2041 aggtctacgc cttcccgcgc atcaaaacga agcacaccca cggcaccggc tgcacgcttg
     2101 cgactgccat tgcgtgcggt ctcgcgcagg gcctgtcgct accgctcgcc gctggccgcg
     2161 cgcgcgaata tgtgcagaac gcgattgaat ccgcacccgg cctcggcaaa ggccgtgggc
     2221 cgctaaatca cgatatttag cgagaaaagc ctgtattcgg aggaagtgac tttttagcca
     2281 tcgaagtcgg atggcggcgg cggtaatacc gtcaacaaat caagtttttt ccagttccga
     2341 tatttgtcca gcattattgt cactttttct tggacgatat cgctgagcag atagcctttg
     2401 ctccagccga caatgtgatc ccagactcga cctgaatgac caagacagtc tgacaacatc
     2461 aattgagcaa ttgcggtatc gtcattcaaa agccgccacg aaccaggaac agggtgtccc
     2521 ccgagttcgc aatgatatga atagtccaca cttcgaaaac gaccttccgc tgctttccgc
     2581 aatttagctg gcgtaaagaa cgattggcga tcttcgcgac tacttcgcag ccatcgggcg
     2641 gattcggcgt cttttgtttc aaatgcccag gctaggtact ccacctccac aagctgtcga
     2701 agcaacgcac aa
//
LOCUS       DASZMC010000087         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_314938,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000087 DASZMC010000000
VERSION     DASZMC010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314938"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..469
                     /locus_tag="VG274_01670"
     CDS             197..469
                     /locus_tag="VG274_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWE05384.1"
                     /translation="MITAAQLRAARALLGVDQRQLAELCGLSVPTVQRMEASDGVIRG
                     NVDSLMKVVAALDKAGLELIGEGAASAGGGRGVRLKTQASNNRQGR"
     gene            469..2469
                     /gene="hyfB"
                     /locus_tag="VG274_01675"
     CDS             469..2469
                     /gene="hyfB"
                     /locus_tag="VG274_01675"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HWE05385.1"
                     /translation="MALICELWSVAALLGLAILAVPIGRSRFASYIIYGSSFAIAAFA
                     FVVSATQIAAAPVDLILPVGLPWLGAHFHLDALSAFFLAVVNLGAAIASFYAIGYGRH
                     ETEPARVLPFYAAFLAGMNLVIVSADAFSFLFAWEFMSLSSWGLVVSHHRDRENVRAG
                     FVYLIMAGFGTFCLLLAFGLLAGAGGGYVFDQIRHVHDKALAPFVLLLALLGAGSKAG
                     LVPLHVWLPLAHPAAPSHVSALMSGVMTKVAVYAFVRIVFNLLGPPAWWWGVVVLVLA
                     AITCMLGVLQALMQHDLKRLLAYHTVENIGIVFIGLGLALAFRANGMVFAAALAMTAA
                     LLHVLNHSVFKSLLFFGSGAVLSATGERDMEHLGGLIHRMPQTAFAFLVGAAAISALP
                     PLNGFASEWLTFQSIFLGPKLPQWGLLFLVPCVGAALALSAALAAACFVKAFGITFLG
                     RPRSERAATAGETDRFSLAAMFGLAVLCLLLGIFPGLAIDWISPATMQLVHAHMPEQR
                     SIAWLTIVPIAQGRSSYNGLLVFLFIAASACFAISAIHRFASHAVRRAPAWDCGTPNP
                     SPLTQYTAGSFAQPIRRVFGPVVFRAREHVRMPPPGDMRPAQFAAELHDIPWETLYAP
                     IDRAVAFAADRLNALQFLTIRRYLSLVFFLLIALLLGIAIWP"
     gene            2648..>3247
                     /locus_tag="VG274_01680"
     CDS             2648..>3247
                     /locus_tag="VG274_01680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HWE05386.1"
                     /translation="MIRDLIVQGLEMALVLLLAPLLTGFVRKARAHFQRRRGASVFQP
                     YRDLARLLRKEVVLAENASWLFRTAPYLIFAATWVAAALVPTFATGLMFSWCADLIAI
                     IALLGSARFFLALAGLDIGTSFGGIGSSREVMIASLAEPAMIMMVFTLALIAGSTQLS
                     TIAHAMTFSHVGLRVSLAFALVALVLVAIAENGRIPVDNP"
BASE COUNT          449 a         1067 c         1002 g          729 t
ORIGIN      
        1 cgtggcgcct tggcggttca tctctcggat acgcctgcgg tttcgtggtg ctgaacgcaa
       61 tgtggctcga gcagtgcagc gcgacggccc ggcggcatct tggggatttg aattattcgc
      121 tacacaacat gcttattgat acttgacgca tcagaattaa gtatttatac atgagttgac
      181 ttcgattcgg gttctcatga tcacggctgc gcaattgcgt gcggcgcggg cgctgcttgg
      241 cgtcgatcag cgccaattgg ccgagctttg cggcctgtcg gtgcccaccg tccagcgcat
      301 ggaagcaagc gacggcgtca ttcgcggcaa tgtggattcg ctgatgaagg tggtcgcggc
      361 gctcgacaag gccggacttg aactcatcgg cgaaggcgca gcaagcgcgg gcgggggccg
      421 cggcgtgcgg ttgaaaacgc aggcttcaaa caaccgtcag ggccgctgat ggcgctgatt
      481 tgtgaattgt ggagcgtcgc cgcacttctg ggcctcgcca tattggcggt tccaatcggg
      541 cggtcgcgct tcgcttcgta cattatatat ggctcgagct tcgccatcgc ggccttcgcc
      601 ttcgtggtgt cggcgacgca gatcgcggcc gcgccggtcg acctgatcct tccggtcggc
      661 ctgccctggc ttggggcgca ttttcatctg gacgcgctct ccgcattctt tctcgccgtc
      721 gtcaatctcg gcgcggcgat cgcaagcttc tacgcgatcg gttatggccg gcacgagacg
      781 gagccggccc gcgtgcttcc gttctatgcg gcgtttctcg ccggcatgaa tctcgtcatc
      841 gtctccgccg acgcattcag cttcctgttc gcctgggaat tcatgtcgct cagttcctgg
      901 ggattggtag tgtcccatca tcgcgaccgc gaaaatgtgc gcgcgggctt cgtctacctc
      961 atcatggcag gcttcggcac gttctgtctc ctgctcgcct ttggattgct ggccggcgcc
     1021 ggcggcggtt acgtcttcga ccagattcgc catgtgcacg acaaggcgct tgcgccgttc
     1081 gtgctcctgc ttgcgcttct gggcgctggt tcgaaggcag gcctcgttcc gctccatgtt
     1141 tggctgccgc tcgcccatcc ggctgcgccc agccacgtct cagcgctgat gagcggggtg
     1201 atgaccaagg ttgcggtcta cgcattcgtg cggatcgtgt tcaatctgct cggcccgccc
     1261 gcctggtggt ggggcgtcgt agtcctcgtg ctcgcagcga tcacctgcat gctcggcgtc
     1321 ctgcaggcgc tgatgcagca cgatctcaag cgcctgcttg cctatcacac ggtggaaaac
     1381 atcggcatcg tcttcatcgg cctcggcctc gcgctggcgt tccgcgccaa cggaatggtc
     1441 ttcgccgcgg ctcttgcgat gacggcggcg ctcctgcatg tgctcaacca ttccgttttc
     1501 aagagcctgc tgttcttcgg ttcgggcgcc gtcctctccg caaccgggga acgcgacatg
     1561 gagcatctcg gcgggctgat tcaccgcatg ccgcagaccg ccttcgcgtt tctcgtcggc
     1621 gcagcagcga tatccgcgct gccgccgctg aacggcttcg cgtccgaatg gctcaccttc
     1681 cagtcgatct ttctcggccc gaagctgccg caatgggggc tgttgtttct cgtgccctgc
     1741 gtcggcgcag cgctcgcgct gtccgcggca ctggccgcgg cctgtttcgt caaggccttc
     1801 ggcatcacct tcctcggccg gccgcgcagc gaacgggcag ccacagccgg cgagacggat
     1861 cgcttctcgc ttgcagcgat gttcggactg gctgtgctgt gtctcctgct cggcatcttt
     1921 ccggggctcg ccatcgactg gatttcgccg gccaccatgc agctcgtgca tgctcacatg
     1981 cccgagcagc gctccattgc atggctgacg attgttccga ttgcgcaggg ccgcagttcc
     2041 tacaacgggc tgctggtttt cctcttcatt gcggcgtccg cgtgcttcgc gatctcggcg
     2101 attcatcgct tcgcctcgca tgcggtccgc cgcgcgccgg cctgggattg cggaacgccg
     2161 aacccaagcc cgctcacgca atacaccgcg ggaagcttcg cgcagcccat tcgccgcgta
     2221 ttcggtcctg tcgtttttcg cgcgcgggag catgtgagaa tgccgccacc gggcgacatg
     2281 cggccggcgc agtttgccgc cgaactgcac gacatcccgt gggagacgct ctatgcgcca
     2341 atcgaccgtg cggtcgcctt cgccgccgac cggttgaacg cgctgcagtt cctgaccatc
     2401 cgccgttatc tgagcctcgt gttctttctg ctgatcgcgc tgcttctggg gatcgcgata
     2461 tggccgtaat ccccaaaaag attttaacgc agagggcgca gaggaagcgc aggggacgca
     2521 gagaaagaca tggcgcgcga agcgcgcaac tgttccacag cgtcctctgc gaatcccctg
     2581 cgccctctgc gttaagagct ttttcctttt tttccacctc cgaacctatc gggaggaggc
     2641 gggggcgatg attcgcgatc tcatcgtgca ggggctcgag atggcgctgg tgctgttgct
     2701 cgcgcccctg ctcaccggct tcgtgcgcaa ggcccgcgcg catttccagc gcaggcgggg
     2761 ggcctcggtg ttccagcctt accgcgatct cgcgcgcctt ctgcgcaagg aggtcgtgct
     2821 cgccgagaat gcatcgtggc tgttccgcac cgcgccctat ctgatcttcg ccgcgacctg
     2881 ggttgccgcg gcgctcgtgc cgaccttcgc caccggcctg atgttcagct ggtgcgccga
     2941 tctgatcgcc atcattgcgc ttctgggcag cgcacggttt ttcctggcgc tggccggcct
     3001 cgacatcggc accagcttcg gcggcatcgg ttcgagccgc gaggtgatga tcgcctcgct
     3061 cgccgagcct gcgatgatca tgatggtgtt cacgctcgcg ctgatcgcag gctcgaccca
     3121 gctctcgacc atcgcgcatg cgatgacatt ctcccatgtc gggttgcgcg tctcgctcgc
     3181 cttcgcgctc gtggcgctcg ttctcgtcgc catcgccgag aacggccgca tccctgtcga
     3241 caatccg
//
LOCUS       DASZMC010000088         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1691420,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000088 DASZMC010000000
VERSION     DASZMC010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691420"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1798)
                     /locus_tag="VG274_01685"
     CDS             complement(134..1798)
                     /locus_tag="VG274_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWE05387.1"
                     /translation="MQFSVPHLLDRCDEAVFAAEGLLAAAKDRVRARVETDGTVSAAL
                     LDREQRAAHALAWFATTVEVLRQMARWARELAQENRLHEFEPLILAAAFGEYLAQLSG
                     GIAMSQNEIARAGDMGLDAEEIGEFLSATVRELIALGTARETRAAISDYMSAQEGAIC
                     FGDPGLDDTMTMIREQFFRFAQEKIAPHAHAWHLRDELIPLETIAEMSALGVFGLTVP
                     EEFGGAGLSKTAMCVVSEELSRGYIGAGSLGTRSEIAAELILAGGTKEQRTHWLPLIA
                     SGAKLPTAVFTEPNTGSDLGALTTRAVRDGGVYRVFGNKTWITHAARADFMTLLARTD
                     PSTRDHRGLSMFLAEKPRGTAADPFPVQGLSGGEIGVLGYRGMKEYEIRFDGFEVPAE
                     KLLGCEEGNGFKQLMQTFESARIQTAARAIGVAQCALDLGLRYALAREQFGHRLFDFP
                     RVQDKLAMMAVEIMAARQITYYAAREKDEGRRCDLEAGMAKLLAARVAWAAADNALQI
                     HGGNGFALEFPVSRVLCDARILNIFEGAAEIQAHVIARRLLEHGVN"
BASE COUNT          350 a          656 c          584 g          375 t
ORIGIN      
        1 accgctcgct gcttccgtcc agatcctctc gactcatcca ggctctcctg ccaaaaatga
       61 atcttctcga cttatctgag tctcaaacgc accggtctgc ggctttctga ctcacctttc
      121 tgactcagta agattaattg acgccgtgtt ccagcaggcg gcgggcgatg acgtgggcct
      181 gtatctcggc ggcgccttcg aaaatgttga ggatgcgggc gtcgcagagc acgcggctga
      241 ccggaaactc gagcgcgaag ccgtttccgc cgtgaatctg cagcgcgttg tcggcggcgg
      301 cccaggcaac acgcgccgcc agaagcttcg ccatgcccgc ttcgagatcg caacgccttc
      361 cctcgtcctt ctcgcgcgcc gcgtaatagg tgatctggcg cgccgccatg atctccaccg
      421 ccatcatcgc cagcttgtcc tgcacgcgcg gaaaatcgaa caggcgatgg ccgaactgct
      481 cgcgcgcaag tgcgtagcgc aagccgaggt cgagcgcgca ttgcgcgact ccgattgccc
      541 gcgccgccgt ttgaatgcgc gcggattcga acgtctgcat gagctgttta aagccgttgc
      601 cctcctcgca tcccagcagc ttttccgccg gcacctcgaa accgtcgaag cggatttcgt
      661 attccttcat gccgcgatag ccgagcacgc cgatttcgcc acccgaaagt ccttgcaccg
      721 gaaacggatc ggcggccgtt ccgcgcggtt tttcggcgag aaacatcgac aatccgcgat
      781 ggtcgcgcgt cgatggatcc gtgcgcgcca gcagagtcat gaaatcggcc cgcgcggcgt
      841 gggtaatcca ggtcttgttg ccgaatacgc gatagacgcc gccgtcgcgc acggcgcgcg
      901 tcgtgagcgc gccaagatcg gaaccggtgt tcggctcggt gaagaccgcg gtcggcagtt
      961 tggcgccgct ggcgatgagc ggcagccagt gggtgcgctg ttctttcgtg ccgcccgcca
     1021 atatcaattc tgccgcgatt tccgatcgcg tgccgagcga gcccgcgccg atatagccgc
     1081 ggctcaattc ctcggagacg acgcacattg cggtcttcga cagtccggcg ccgccgaatt
     1141 cttcgggaac cgtcagcccg aaaacgccca aagcgctcat ctccgcgatc gtttcgagcg
     1201 gaatcagttc gtcgcgcaga tgccaggcgt gcgcatgggg cgcgatcttc tcctgcgcga
     1261 agcggaagaa ctgctcgcgg atcatcgtca tcgtgtcgtc gagacccggg tcgccgaagc
     1321 agatggcacc ctcttgtgcc gacatgtaat cggaaatggc ggcgcgtgtt tcgcgcgccg
     1381 ttcccagcgc gatcagttcg cgcaccgtgg ccgagagaaa ttccccgatc tcctcggcgt
     1441 cgagacccat atcgccggcg cgcgcaatct cgttctggct catggcgatg ccgcccgaga
     1501 gctgggcaag atattcgccg aatgccgccg ccagaatcag cggctcgaat tcgtggagac
     1561 gattctcctg cgcgagctcc ctggcccagc gtgccatctg gcggagcacc tccaccgtcg
     1621 tcgcgaacca tgccagcgcg tgcgccgctc gctgttcgcg gtcgagcagc gcggccgaca
     1681 ccgtcccgtc ggtctcgaca cgggcgcgca cgcgatcctt cgctgccgca agcaatccct
     1741 ccgccgcaaa cacagcctcg tcgcaacggt cgagaaggtg gggaacggaa aattgcattg
     1801 gttcaatcgc gatccgctca accaccacag ggtgatcact cggctcttat agctgtttcc
     1861 tcgacgtttt aaaaagcgtg tcatcccgga atcgcgcgac agcgcgatat ccgggaccca
     1921 ggaaaacacg cgcccttgcg ctttacgtgc tgggttccgc tcgag
//
LOCUS       DASZMC010000089         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2522065,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000089 DASZMC010000000
VERSION     DASZMC010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522065"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /gene="trpC"
                     /locus_tag="VG274_01690"
     CDS             <1..706
                     /gene="trpC"
                     /locus_tag="VG274_01690"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWE05388.1"
                     /translation="RADLERRAADRPAPRDFHAALLASPPAIIAEIKQASPSKGVFTD
                     DFHPASIAQLYEAGGAAALSILTDEEFFKGKLADLEAARSAVAIPVLRKDFTIDEFHV
                     IEAAAHGADAILLIAAILDVKQLRGFRELAAQYGMAALVEVHDDYELASALDSGAEIV
                     GVNNRNLHTFDVTLDTSLRLAEKMPAAVVKIAESGIQSRADVQRLQAAGFHAFLVGEH
                     LMKSADPAAGLRELRS"
     gene            706..1266
                     /locus_tag="VG274_01695"
     CDS             706..1266
                     /locus_tag="VG274_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWE05389.1"
                     /translation="MVKVCGITRREDALVAAEAGASAIGFIFYPQSPRYIAPERAAAL
                     GEGLDLWKVGIFVDEAPWDIEAAMHAAHLDVAQIYGGDAPAGARVWKAFRIADSVEGP
                     FGEGAEAVFLDSAKNGLSFDWKLARNAGEKVIVAGGLDAFNVAEAIRIAEPWGVDASS
                     KLESAPGIKDHDKVRAFVKAAREAIW"
     gene            1260..>2369
                     /gene="trpB"
                     /locus_tag="VG274_01700"
     CDS             1260..>2369
                     /gene="trpB"
                     /locus_tag="VG274_01700"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWE05390.1"
                     /translation="MVISQPDATGHFGPYGGRFVPEVLMAPLEELEHAYHKAAADPSF
                     QAELDDLLHNYAGRPTPLYYAKRLSEMLGGAKIYLKREDLLHTGAHKINNCLGQALLA
                     RRMGKKRIIAETGAGQHGVATATVCALFGLECIVYMGVEDMRRQQLNVFRMRLLGAKV
                     VGVVNGSQTLKDAISEAMRDWVTNVHDTYYLLGSALGAHPYPVMVRDFHRVIGVEARA
                     QILEQEGRLPDSAFACVGGGSNAIGLFHAFIGDESVKLIGVEAGGRSALLGQHAARFS
                     GGSPGVLHGTLSYLLQDDDGQVALTHSVSAGLDYPTVGPEHAWLHDRHRAEYTSASDP
                     EALAAALQLARTEGIIPALESAHAIAEVIKRAPAAK"
BASE COUNT          434 a          734 c          764 g          437 t
ORIGIN      
        1 ccgcgccgat ctggagcgcc gcgccgccga ccgtcctgcc ccgcgagatt tccacgccgc
       61 cctccttgca tctcctccgg cgatcatcgc cgagataaag caggcgtccc cgagcaaggg
      121 cgtttttacc gatgactttc accctgcctc catcgcccag ctctacgaag caggcggcgc
      181 cgcggccttg agcatcctga ccgatgagga atttttcaag ggcaagctgg ccgatcttga
      241 ggccgcccgg tcggccgtcg cgattccggt tctgcgcaag gacttcacca tcgacgaatt
      301 ccatgtcatc gaagccgctg cgcacggcgc cgacgcgatt ctactgatcg cggccattct
      361 ggatgtcaag caactgcgcg gatttcgcga actcgcggcg cagtacggaa tggcggcgct
      421 ggtcgaggtt cacgatgact acgagctcgc ctcggcgctg gattccggcg cggagatcgt
      481 tggcgtgaac aatcgcaact tgcatacgtt tgacgtcacg ctcgatacat cactgcgcct
      541 ggcggagaag atgccggctg cggtggtgaa gatcgcggag agcgggattc agtcgcgcgc
      601 cgatgtgcag cggctccagg ccgccgggtt ccacgcgttt ctagtcggcg agcatctgat
      661 gaaatccgcg gaccccgccg cggggctgcg ggaattgcgg tcatgatggt caaggtctgt
      721 ggcattaccc gccgcgaaga cgcgctagtg gcggccgaag ccggggcgtc cgccatcgga
      781 tttatctttt atccgcaaag cccgcgctat atcgcgcccg agcgggcagc cgcgttgggc
      841 gaaggactcg atttgtggaa agtcggcatt tttgtagatg aagcgccctg ggatatcgaa
      901 gccgccatgc atgccgcgca tctggatgtc gcgcagattt atgggggcga cgctccggct
      961 ggcgcgcgcg tctggaaagc gttccgcata gccgattcgg tcgagggccc atttggcgag
     1021 ggcgccgagg cggtatttct cgacagcgcg aagaatggtc tatcgttcga ctggaagctg
     1081 gcccggaacg caggcgagaa ggtcatcgtc gccggcggcc tcgacgcatt caatgtagcg
     1141 gaagcgatcc gcatcgccga gccctggggg gtcgatgcca gctcgaagct ggagtccgcg
     1201 ccgggcatca aggatcacga taaagtacgg gcctttgtga aggctgcgcg agaggcaata
     1261 tggtaatttc ccaacccgac gccaccggtc acttcggacc ctacggcgga cgatttgttc
     1321 ccgaagtgtt gatggccccg ctcgaagaac tcgagcacgc gtatcacaag gccgcagccg
     1381 atccgagttt tcaggcggag ttggacgacc tgctgcacaa ctatgcggga cgtcccacgc
     1441 cgctttacta cgccaagcgc ctgagcgaaa tgctcggcgg cgcgaaaatt tatctcaagc
     1501 gcgaggatct gctgcacacc ggcgcgcaca agattaacaa ctgcctgggg caggctctgc
     1561 tcgcccgccg catgggcaag aaacgcatta ttgccgagac tggcgcgggg cagcatggcg
     1621 tcgccaccgc taccgtgtgt gcgctgttcg gcctcgaatg catcgtctac atgggcgttg
     1681 aggacatgcg ccgccagcag ctcaacgttt ttcggatgcg cttgctggga gccaaagtcg
     1741 tcggcgtcgt caatggcagc cagaccctca aggacgcaat cagcgaagcg atgcgggact
     1801 gggtcaccaa cgtccacgac acgtattacc tgttgggttc cgcgttgggc gcgcatcctt
     1861 atccggtgat ggttcgcgac tttcatcgcg tgatcggtgt agaagcgcgc gcgcagattc
     1921 tggagcagga aggccgcctc ccggattcag ctttcgcgtg cgtcggtgga ggcagcaacg
     1981 ccatcggcct gttccatgcg ttcatcggcg atgagagcgt caagttaatc ggcgtcgaag
     2041 cgggcggacg gagcgcgctg ctcggccagc acgcggcgcg tttctcgggc ggatcgccgg
     2101 gcgttctgca cggcacgctc agctatctgc tgcaggatga cgatgggcag gtggcgctta
     2161 cacattcggt ttcggcaggc ctggattacc cgaccgtggg acccgagcat gcctggctgc
     2221 acgatcggca ccgcgccgaa tacacgtccg ctagcgatcc cgaagcgctc gcagccgccc
     2281 tgcaactagc gcgcacggaa ggcatcatcc cggcgctcga atccgcgcac gcgatcgccg
     2341 aagtcattaa gcgcgccccc gcggccaag
//
LOCUS       DASZMC010000090         4725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_40209,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000090 DASZMC010000000
VERSION     DASZMC010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40209"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VG274_01705"
     CDS             <1..1095
                     /locus_tag="VG274_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05391.1"
                     /translation="GGAETTYPEWQSVLATLSPPKEQFAYKPVYKDASTRIAETADAQ
                     PKRAPAYDKVEPLHVAGNVFMIGGAGANIAVSAGGDGIIMVDSGAAEASDKVLAAVRK
                     ASEALVPPEQPDSASPFGDTWQVTHAFRDPVIRMIINTSDSLDHVGGNANIRMSPLFR
                     PLGADGGANIAVIVAHENVQNRMADRKAPEAALPTSVYSSDKYTIHRFLNNQAIQLVH
                     MTNATTDGDSAVFFRRSDVLATGDVYNSDVYPPIDVDRGGSIEGEIESLNRLTDMCVE
                     EFMSQGGTLIVPGHGWLSDAGDLGYYRDMIIIVRDRVQKMIDSGMTLAQVKAAKPTLD
                     YDPEYGREPGVTARFVEAVYRSLTEKKKAK"
     gene            1146..1544
                     /locus_tag="VG274_01710"
     CDS             1146..1544
                     /locus_tag="VG274_01710"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWE05392.1"
                     /translation="MKRTLLLICAAALLTCVPGLAHHSFAGTYDAAKEITIKGKIVEV
                     SLRSPHSFFFVESEDANGVAQRWSLEGASAAQFAQQGVTRDALKVGDVVEVIGNPARS
                     MTTGSRARLLKITRPSDGWSWGTRPGETVR"
     gene            1602..2486
                     /locus_tag="VG274_01715"
     CDS             1602..2486
                     /locus_tag="VG274_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05393.1"
                     /translation="MRCPVVMLCVAGACLFAQEGGRGGRGGPPPTAKASAPIDLTGYW
                     TAVITEDWHLRMQTAPKGDFGVGAPGAVVLPGGAAYGLGPNPSDGGNIPYKTKGGQAA
                     MKWDAAKDEADGNQCRAYGAPGVMRLPTHLHVTWKDDNTLQIDADAGSQTRDLHFVPP
                     PQGGQINFQSGAYIPPEAVKMEPPPGAQASWQGYSIAQWTIMGGGRGYDRSGSLKAIT
                     THLKPGYYWKNGMPYTEHAELTEHFRTLGLPDGSQWIILVQMVEDPEYLTQPFVVNYH
                     FKKLADGSKWNPTPCSAR"
     gene            complement(2529..4421)
                     /locus_tag="VG274_01720"
     CDS             complement(2529..4421)
                     /locus_tag="VG274_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWE05394.1"
                     /translation="MDARTRALGDSSREVGGLLLGHQGRNDSVVVVDDYVWLPSEAMG
                     SYTLNTAELERLRGERAGESSVVIGYFRTDFGASLELRDAEIDLIGEQFSEPTNIVLL
                     IGSSRPHTSGFFFWMGEGVISPFSLMDFPLDATRLPFHDDAATEPFAVPSASLAELLA
                     AAESSIEPPSIEPPFLPPLAAASDVELVKWPVLEPVVKKGAPSATVVQMPSKSAPIPA
                     APVVTIAQPPAEKISPAAPSREKTPHKNGSSKDNASRNNSGDPASREKPASENPAHAA
                     AAPSRAKGVWVTIAWTLALLIGLCLSAGVVVLVFRGDPLSFLRPSPVVKSTPQPAPAF
                     PFRLTVEAQGTGLNIDWDAQSPAVAQAHEGQLTITETGTPAVVIPLTTQQLTIGHIYF
                     QSSAERVEVQLETLDATGKPSRESVLAFSPVPRPSAPAVASVQQPKPAAPAPQPAWLP
                     ARKVETFAIPGRNVSATEAPEVPRVERSASRAFKAPSLNRTPSRLPVIDQPATAWPPQ
                     SLAVPMPLNTGVLSTRVAPPPQQTAPQRIKVTSSLQAARLVRKVAPVYPQVAIAGRVQ
                     GEVRFTAIIARDGTVRDLKQTAGPSILGKAATAAVNQWTYQPTLLDGQPVEVATEIDV
                     NFTLSQ"
BASE COUNT          936 a         1329 c         1618 g          842 t
ORIGIN      
        1 ggcggcgcgg agaccacgta tccggaatgg cagagcgtgc tggcgacgct atcgccgccg
       61 aaggagcagt tcgcgtacaa gccggtatat aaggatgcat cgacgcgcat cgcggaaacg
      121 gcggatgcgc agcccaagcg cgcgccggcc tatgacaaag tggagccgct gcacgtggcg
      181 ggcaacgtat tcatgatcgg cggcgccggc gcgaatatcg cggtgtccgc cggaggagac
      241 ggaatcatca tggtagattc cggcgcggcg gaggcgagcg ataaagtcct ggccgcggtg
      301 cgcaaggcat ccgaagcgct ggtgccgccg gagcagccgg attccgcatc gccgttcgga
      361 gacacgtggc aggtgacgca cgcgtttcgc gatccggtga tccgcatgat tatcaacaca
      421 agcgacagcc tggatcatgt gggcggcaac gcgaatatcc ggatgtcgcc actgtttcgt
      481 ccgctgggcg ccgatggagg cgcgaacatc gcggtgattg tggcgcatga gaacgtgcag
      541 aatcgcatgg cggaccggaa ggctccggaa gcggcgctgc ccaccagcgt gtattcgtcg
      601 gacaagtaca cgatccatcg cttcctgaat aatcaggcga ttcaattggt gcatatgacg
      661 aacgccacta cggatggcga cagcgccgtg ttctttcggc ggtcggacgt gctggcgacg
      721 ggcgacgttt ataacagcga cgtgtatcca ccgattgatg tggaccgcgg cggcagcatt
      781 gaaggcgaga tcgagtcgct caataggctg acggatatgt gcgtggagga atttatgtcg
      841 cagggcggca cgctcattgt tccgggccat ggatggctga gcgatgcggg cgatttgggc
      901 tattaccgcg acatgatcat tattgttcgc gacagggttc agaagatgat cgattcggga
      961 atgacgctgg ctcaggtaaa ggccgccaag ccgacgctgg attacgatcc cgagtatgga
     1021 cgcgaaccgg gcgtcacagc tcgcttcgtg gaggccgtgt atcgcagcct cacggaaaag
     1081 aagaaggcca agtagaacag aaggcatcgc gtgaaacagg agacgccgtg aaacaggaga
     1141 cacccatgaa acgaacgttg cttctcatat gcgccgcggc gctgcttaca tgcgttcccg
     1201 ggctggccca ccactcgttt gccggaacct atgacgccgc caaagagatc acgatcaagg
     1261 gcaagattgt cgaagtctcg ctgcgatcgc cgcattcctt cttcttcgta gaatccgagg
     1321 acgcgaacgg cgtggcgcag cgctggtcgc tagaaggcgc gtcggcggcg cagttcgcgc
     1381 aacagggcgt gacgcgcgat gcgctgaagg tgggcgacgt ggtggaagtg atcggcaatc
     1441 ccgcgcgcag catgaccacg ggttcgcgcg cgcgtctatt gaagatcacg cgtccgagcg
     1501 atggctggtc gtggggcacg cgtccgggcg agacggtgcg gtagcggcac cgaaaacaac
     1561 ggagaaacat gaaccgccga atgccagtgc gccgaaggcc agtgcgatgt cccgttgtca
     1621 tgctgtgcgt ggcgggcgcg tgtttgttcg cgcaggaagg gggacgcggc gggcgcggag
     1681 ggccgccgcc cacggcgaag gcttcggctc ctatcgatct gacaggctac tggacggcgg
     1741 tgattaccga agactggcac ctgcgcatgc agacggcgcc gaagggcgat ttcggcgtcg
     1801 gcgctccggg agcggtggtg ctgccgggcg gcgcggcgta cgggctgggg ccgaatccgt
     1861 cggacggcgg caacatccct tataaaacga agggcgggca ggccgcgatg aagtgggatg
     1921 cggcgaagga cgaggccgat ggcaatcagt gccgcgcgta cggcgcgccg ggcgtgatgc
     1981 ggctgccgac gcatttgcac gtgacgtgga aggacgacaa cacgctgcag atcgatgcgg
     2041 acgcggggtc gcaaacacgc gatctccatt ttgttccgcc gccgcaggga gggcagatca
     2101 attttcaatc gggcgcatat attcctccgg aggccgttaa gatggagccg ccgccgggag
     2161 cgcaagcgtc gtggcaggga tattcgatcg cacagtggac gatcatgggc ggcgggcgcg
     2221 ggtatgatcg cagcggcagc ctgaaggcga tcaccacaca cctgaagccg ggctattact
     2281 ggaagaacgg aatgccttat acggagcacg ccgagctgac ggaacatttt cggacgctgg
     2341 gcctgcccga tggcagccag tggatcatcc tggtgcagat ggtggaagat cccgaatatc
     2401 tgacgcagcc gttcgtggtg aactatcact tcaagaagct ggcggatgga tcgaagtgga
     2461 atcctacgcc gtgctcggcg cgctgacccc ctgagggagg aatcgcggcg cggttcgctg
     2521 tgacttcgtt attgcgacag cgtgaagttg acgtcgatct ccgtcgccac ttccacgggc
     2581 tgcccatcga gcagcgtggg ttgataggtc cactgattga cagcggcggt ggcggccttg
     2641 cccagaatcg aggggccggc cgtctgtttc agatcgcgaa cggtgccgtc ccttgcgatg
     2701 atggccgtga accggacctc gccctgcacg cgtcccgcga tggcaacctg aggatagacg
     2761 ggcgcgacct ttcgcacgag gcgcgccgcc tgcagcgagc tggtgacctt gattcgttgc
     2821 ggagccgtct gctgcggtgg aggcgcgact cgcgtgctga gaacgccggt attgagcggc
     2881 atcggaaccg cgagcgattg aggcggccag gcggtagccg gttgatcgat gaccggcaaa
     2941 cgcgacggcg tccggtttaa cgagggtgcc ttaaaggcac gcgacgcgga tcgttccacg
     3001 cgcggaacct ccggggcctc cgttgcactt acgttcctgc cggggatcgc aaaggtttcc
     3061 accttgcgcg ccggcagcca agcgggctgc ggtgcgggcg cggcaggttt gggctgttga
     3121 acggacgcga cggcgggcgc ggacgggcgt ggcacggggg agaaagccag aacggattcg
     3181 cgcgacggct tgcccgtggc gtccagcgtt tccaattgca cttccacgcg ttccgcggag
     3241 gattggaaat aaatgtgacc gatggtgagc tgctgcgtgg tgagagggat cacgacggcc
     3301 ggcgtgccgg tctccgtaat tgtgagttgc ccttcgtgcg cttgcgccac cgcggggctt
     3361 tgcgcgtccc aatcaatgtt gaggcccgtg ccctgcgctt ccaccgtcag ccggaacgga
     3421 aaagccggcg ccggttgggg cgtggatttt accacgggcg acggcctgag gaacgacaac
     3481 ggatcgccgc ggaaaacgag aacaacaacg cccgccgaga gacacaggcc gatcagaagc
     3541 gcgagcgtcc acgcgatggt cacccaaacg cctttcgctc tcgatggagc tgccgccgca
     3601 tgcgccggat tttcgctggc gggcttttcg cgggaagctg gatcacctga attgttcctg
     3661 cttgcgttgt ctttgctcga accgtttttg tgtggcgtct tttcgcgcga aggcgctgca
     3721 ggcgaaatct tttcggcagg cggttgggca atggtcacaa ccggcgctgc cgggataggc
     3781 gccgattttg aaggcatctg gacaaccgtt gcggaaggcg cgcccttttt cacaactggc
     3841 tccagaaccg gccacttcac caattccaca tcggacgcgg cggcgagcgg cgggagaaat
     3901 ggcggctcga tggatggcgg ctcgatggac gactcggcgg cggcgagtaa ttcggcgagc
     3961 gacgcgctcg ggacagcgaa aggctctgtc gcggcgtcgt cgtggaacgg caggcgcgtc
     4021 gcgtcgagcg gaaaatccat caacgaaaac ggactgatga cgccttcgcc catccagaaa
     4081 aaaaatccgc tggtgtgcgg acgcgatgaa cctatcagta gaactatgtt ggtgggttcg
     4141 gaaaactgct cgccgatcag atctatctcc gcgtcgcgca gctcaaggct cgcgccgaag
     4201 tcggtacgga agtagccgat caccacggaa gattcgccgg cgcgttcgcc gcgcagccgt
     4261 tccagttccg ctgtgttcaa agtgtaggag cccatggctt cggaaggaag ccacacgtag
     4321 tcgtccacca cgacgacaga atcgttgcgg ccctgatgac cgagcagaag gccgccgact
     4381 tcgcgagagg aatcgcccag ggcgcgcgtg cgtgcgtcca aatcgctctg gaggcgcgcg
     4441 acaaggttgc gtgacaaatt aacgcaaatg ggagcgccgg gaaatgtcca gctccacact
     4501 tgtgctccgg aagccaactc gcttccggag gcgggcctcg cgatagatga cgtactcatg
     4561 cttcgcctca ctgacgcggc tctgcctcag ccgacataag acaactatta ctttccgggg
     4621 tccttcttat tgtgcatcca ggaatcagat tagaaaagat tttggatgaa tttacatatg
     4681 cgtttcgtac taagagttgc gggactgccg ggcgtgtttc cagta
//
LOCUS       DASZMC010000091         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_40503,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000091 DASZMC010000000
VERSION     DASZMC010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40503"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VG274_01725"
     CDS             complement(<1..1416)
                     /locus_tag="VG274_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05395.1"
                     /translation="MSRLPSIHENQRDHIAVEPAQTSSEHLVMSKDAANAARLLREGV
                     VWSILGALITLGLALFFGELPRVFVSMYIFTTLEPAAVGVELVALLSACMLLRARAST
                     RWDLILTLPALGPRAIIILSTGTAVFAAIGHFVVYHDQAQSVDEFMTRFGAAMLSHGD
                     LLAHIPTQWKPFGLALQPLFTFFSPDGDLWGSPYRPVNAALHAIFGFVPGGERFTNPV
                     LAGLSVFLVARIAAQLWPERKDAPFVAALLMAVSAQVLVNGMTYYAMPAHLAFNLAWL
                     LFFLRGRLVGHAIAALIGVFAVGLHQVNFHPLFVIPFLTPVLLSRRWPLALFYGAVYA
                     AALVFWVDWYQIALWWSGVGFTQAADSGGDNFVHRIWEFSRLPGFAAWLFMSTNFARF
                     FSWQSVLLAPLAIIGLRSWREAPPIVRQGVWGLLIGSLPFMFVTPEQGGGWGYRYAHG
                     LIGNIILLSVFGWIQLVPRERM"
     gene            complement(1468..2751)
                     /locus_tag="VG274_01730"
     CDS             complement(1468..2751)
                     /locus_tag="VG274_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HWE05396.1"
                     /translation="MPPDLAQPSTAADTMVACNDAFGSPAALPRFRAIPRQLRSGHFI
                     NRKFVLIAGTALLLLEAAILFLFIAVSHHWLGTMNNPTTTDFASFYAAGSLADAGHAP
                     LAYDLAAHRAAEEQATEPGVNYQYFYYPPVFLFICAALAKLPYLPAFVLLQVLTLPLY
                     LFVGRHTLADRSWGALIGLLAFPAVFWNIGLGQNAFLTAALFGGGLLLIDRRPVLAGL
                     LFGALCYKPHFGLLIPVALAAGGRWRAFAAAATSATSLVLLSVATFGWESWYDSLTIF
                     TGAHATYESGYIDLGAFITPYGAVQLVGGGTGAAYVVQAAASLFAIGLVAAVWRKGLS
                     LPLRAAALLAATIIAVPLALIYDDMLAVLAIAWLVRAGRAVGFLRWEKAILAAIFIAP
                     LFSRNLGTATHIPLAMFATAALVGVIVIRIRFELM"
BASE COUNT          706 a          861 c          948 g          469 t
ORIGIN      
        1 cattctttcg cgcggcacca gttgtatcca gccgaaaacg ctaaggagaa tgatattgcc
       61 aataagaccg tgtgcgtagc ggtaccccca cccgccgcct tgctccggag tgacgaacat
      121 aaacgggaga ctaccgataa gaagtcccca aacgccttgg cgcacaatcg gaggcgcttc
      181 gcgccaagaa cgcaatccga taattgcgag cggagccagt agcacgcttt gccaggagaa
      241 aaagcgcgcg aagttcgtcg acatgaatag ccatgcagcg aaaccaggca accgtgaaaa
      301 ttcccagatc cggtgcacaa agttatcgcc accactgtcg gcagcctgtg taaagccgac
      361 cccggaccac cacagcgcga tctggtacca atcaacccag aacactaggg ctgcggcata
      421 gaccgcgccg tagaacagcg caagcggcca acggcgtgat aaaagcaccg gtgttaggaa
      481 cgggattaca aacagcggat ggaaattcac ttgatggagt cccactgcga agacaccaat
      541 taatgcggct atcgcgtggc cgacaagccg cccgcgcaag aagaacagca gccaggcgag
      601 gttaaaggcg aggtgtgccg gcatggcgta gtaggtcatg ccattgacga gcacctgcgc
      661 cgataccgcc ataagtaagg cggcgacgaa cggcgcatcc ttccgctctg gccagagctg
      721 cgcggctatc cgcgcaacga ggaatacgct taggcctgct aagaccggat ttgtaaatcg
      781 ctcgccgcct ggaacgaagc cgaaaatcgc gtggagagca gcgtttacgg gtcggtaggg
      841 gctgccccag agatcgccgt cgggcgagaa aaaggtgaaa agaggctgca aggctaggcc
      901 gaagggcttc cattgcgttg gaatatgcgc gagcagatcg ccatggctga gcattgcggc
      961 gccgaagcgc gtcatgaatt cgtcaaccga ttgggcttgg tcgtggtaca cgacgaaatg
     1021 gccgattgcc gcaaatacgg ctgtgccggt tgagagtatg ataatcgccc ttgggccgag
     1081 tgccggaagc gttaggatca aatcccatct agtggacgcg cgagcgcgta gcagcataca
     1141 ggcagagaga agcgccacta gttcgacgcc gaccgccgcg ggttcaagcg tcgtgaatat
     1201 gtacatcgaa acgaaaacgc gtggcagttc tccgaagaac agcgcaagcc caagcgttat
     1261 caatgcgcca aggatcgacc aaacgacgcc ttcacgcaaa agccgcgccg cgtttgcagc
     1321 gtctttcgac atgactaagt gctcgctgct cgtctgggcg ggttcgacgg cgatgtggtc
     1381 gcgctggttt tcatggattg agggcagcct actcatacca atactggatt tgcgatggcg
     1441 gtgtctcgat tacgcaattg ccgggaacta catcagctca aaccgtatgc gaatgacgat
     1501 gacccccacc agcgccgccg tcgcgaacat cgcgagcggg atgtgcgtcg ccgtgccgag
     1561 attgcgcgag aacagcggcg cgatgaagat cgcggcgagg atcgccttct cccaccgcag
     1621 gaagccaacg gcgcggccgg cgcggacgag ccaggcgatg gcgagcacgg cgagcatgtc
     1681 gtcgtagatc agggcgagtg gcacggcgat gattgtcgcg gcgaggagcg cggcggcgcg
     1741 cagcggcagg ctcagtccct tgcgccacac ggcggcgacg aggccgatcg cgaagaggct
     1801 cgccgcggct tgcaccacat aggcggcgcc ggtgccgccg ccgaccagct gcacggcgcc
     1861 atacggcgtg atgaatgccc cgaggtcgat ataaccggat tcataggtcg cgtgcgcacc
     1921 tgtgaagatg gtgagggagt cgtaccagct ctcccagccg aaggtcgcaa ccgatagaag
     1981 taccaacgat gtcgccgaag ttgccgccgc cgcgaaggcg cgccatcgtc cgccggcggc
     2041 cagcgctacc ggaatcaaca gcccgaaatg cggcttgtag cagagggcgc cgaagagcag
     2101 cccggcgagc accggccggc gatcgatcag caacagtccc ccgccgaaca gcgccgcggt
     2161 taggaaggcg ttctggccga gaccgatatt ccagaatacc gccgggaagg cgagcaggcc
     2221 gatcagcgcc ccccagcttc gatcggcgag ggtgtgtcgg cccacgaaca agtaaagagg
     2281 cagggtcaga acctgaagca ggacgaaagc cggaagatag ggcagtttcg ccagcgcggc
     2341 gcaaataaat agaaagaccg gcgggtaata gaaatactga taattaacgc ccggctcggt
     2401 cgcctgttct tcggccgcgc gatgggccgc gagatcgtag gcgagcggtg cgtggccggc
     2461 atccgccagg ctgcccgcgg catagaagct tgcgaaatcg gtcgtggtcg gattgttcat
     2521 cgtgcccagc caatgatggc tgaccgcgat gaagaggaag aggatcgccg cctccagcag
     2581 caaaagcgcg gtgccggcga tcaagacgaa tttccggttg atgaaatggc cgctgcgcaa
     2641 ttgccgcgga atcgcgcgga aacgcggcag cgcggcgggg ctgccgaaag cgtcgttgca
     2701 cgcaaccatt gtgtcggcag cagtcgaggg ctgtgccaga tccggaggca tgtaacgcat
     2761 aacccgaaac ggcgcgcgct atgtcaatgc ccctatgtct gcgttgggcg gatccacggg
     2821 tcgccccggg gcgtttgcgg ccaaacggcg gcgctgccgc gtcaatcgca agaacaaaat
     2881 tccccaactt tgtcaggaaa ccaaatcgca aggccgtggt tactcaagca cgcaggtcat
     2941 ggttactaag ccattacttt cgaacattaa ggagatttaa aagc
//
LOCUS       DASZMC010000092         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1959380,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000092 DASZMC010000000
VERSION     DASZMC010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959380"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VG274_01735"
     CDS             <1..814
                     /locus_tag="VG274_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE05397.1"
                     /translation="ARFHSDSLVVPAVAPHAPYTNSDETLKAVRALAARYGAPVLIHL
                     SETKKENDDSQAQFHMTPTQRLNALGFFNGRTVAAHCVWMDPKDLDILRARNVGVAHC
                     PSSNMKLASGAAPIIDMLKRGIAVGLGTDGPAGSNNDFDMFEEADLAAKLQKLVRSDP
                     EALPATKAFEMATIGGARVLGLEKEIGSIETGKRADIIAIDLTAPNAVPMYDVYSQLV
                     YALKASNVTDVIVNGREVVRDRRCLTLDSRTVIAKAQEYGQKVAASLKKRAY"
     gene            complement(811..1044)
                     /locus_tag="VG274_01740"
     CDS             complement(811..1044)
                     /locus_tag="VG274_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05398.1"
                     /translation="MKFALLVLVIAAIAAVADSDLSRERREARIEASREARAASAEAR
                     EMARQARNEARQAAREAARDAAREAREAARNWN"
     gene            complement(1090..1851)
                     /locus_tag="VG274_01745"
     CDS             complement(1090..1851)
                     /locus_tag="VG274_01745"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWE05399.1"
                     /translation="MLTNSARRKLEEEGYFVLPGLLSDAEVECFRERVDWLYENEGDG
                     AGAEFKQEPGARRLANVVDKGAIFEKAIETPEVLECVEAVLGPDFKLSSLNVRSANPH
                     NGTEQPLHVDGGELPDARGNSVCNSVWMLDDFTEWNGALRIVPGSHRWGRQPLPGEKA
                     DSEMLVTGKAGTVVVMNAHMWHGGTANRTDRQRRAMHIYYARGDKPQQQYQKRWVRPE
                     VQARLSPLGRKLLALDDPRNDELCATGSGKSGFMK"
BASE COUNT          366 a          717 c          486 g          349 t
ORIGIN      
        1 cgcgcgcttt cattccgatt ctctggtggt acccgccgtc gcaccgcacg cgccctacac
       61 caactccgac gaaacgctca aagccgtccg cgcgctcgcc gcccgctacg gcgccccggt
      121 tctaattcac ctttcggaaa caaaaaaaga gaacgacgac agccaggccc agttccacat
      181 gactcccacg caacggctga acgcgcttgg ctttttcaac ggacgcaccg tggccgcgca
      241 ctgcgtctgg atggacccca aagatctcga catcctgcgc gcaagaaacg tcggcgtcgc
      301 ccactgccct tcgagcaaca tgaagctcgc gagcggcgcg gccccgatta tcgacatgct
      361 caagcgcggc atcgcggtcg gactcggcac cgacggtccc gccggcagca acaacgattt
      421 cgatatgttc gaagaggcgg atctcgcggc caagctgcaa aagctggtcc gctccgatcc
      481 cgaagcacta cccgccacca aagcgttcga gatggcgacc atcggcggcg cccgcgtgct
      541 cggcctcgaa aaagaaatcg gctcgattga aaccggcaaa cgcgccgata tcatcgcgat
      601 cgatctgacc gcccctaatg ccgtccctat gtacgacgtc tattcgcagc tcgtttacgc
      661 cctgaaagcc tcgaatgtga ccgatgtgat cgtgaacgga cgcgaagtcg tgcgcgaccg
      721 ccgctgcctc accctcgaca gccgcacagt gatagcgaag gcccaggaat acgggcaaaa
      781 agtcgccgcc tcgctaaaga agcgagctta ttaattccaa ttgcgcgccg cctcgcgcgc
      841 ctcgcgtgcc gcatcgcgag cagcctcccg cgcggcctgc cgcgcttcgt tgcgcgcctg
      901 acgcgccatt tcccttgctt cggcgctcgc cgcccgcgcc tcgcgactcg cctcaattcg
      961 agcttcacgc cgttcgcggc tcaaatccga atccgccacc gccgcaatcg cggctataac
     1021 caggacgagc aaggcaaact tcataatggt ctatacgcgc cgcccgccaa aaagtttgcc
     1081 gcgcgctcgc tatttcatga aaccgctctt gccggacccc gtcgcgcaca actcatcgtt
     1141 tcgaggatcg tccagcgcca gcagtttccg tcccagcgga ctcaacctcg cctgcacctc
     1201 cggccgcacc catctcttct gatactgctg ttgcggctta tccccgcgcg cgtaataaat
     1261 atgcatcgcg cgccgctgcc ggtcagtccg gttcgcggtt ccgccgtgcc acatgtgcgc
     1321 gttcatcacc acaaccgtcc cggccttccc ggtgaccagc atctccgaat ccgccttttc
     1381 gcccggcagc ggctgccggc cccaccgatg cgaccccggc acgatccgca gcgcgccgtt
     1441 ccactccgtg aagtcgtcca gcatccacac ggaattgcaa accgaattcc cgcgcgcatc
     1501 cggcagctcg ccgccatcca catgaagcgg ctgctcggtc ccgttgtgcg gattggccga
     1561 ccttacattg aggctgctca gcttaaaatc ggggcccagc acggcctcca cgcactccag
     1621 cacctccggc gtttcaatcg ccttttcgaa aatcgcgccc ttatcgacta cattggccag
     1681 ccgccgcgcg ccgggttcct gcttgaattc cgcgcccgcg ccgtcgccct cgttctcata
     1741 gagccagtcg acccgctccc ggaagcactc gacttccgca tcggaaagca gacccggcaa
     1801 tacgaaataa ccttcctctt caagctttcg tctggcggaa ttcgtcagca tgttctctct
     1861 ccagccgcag cagattgtct ttgcgatcca agccgccgcc atagccgccc atcgaccc
//
LOCUS       DASZMC010000093         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4181632,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000093 DASZMC010000000
VERSION     DASZMC010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4181632"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VG274_01750"
     CDS             complement(<1..831)
                     /locus_tag="VG274_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWE05400.1"
                     /translation="MTHIAELVNSGKFETGSKPLSSTRSLKTRAYLGVAALVVTAGLG
                     FAVWHSHAGGAPAPAAPAPIPVTVAAVGAKPVRLWSEYSGRMTAVDSADVRPEVSGRI
                     TEIRFHDGQEVHAGDILLVIDPRTYEAAVAKAQADLATGVTNARLARTELERAKRLMD
                     AQAVARDLYDQRANASAVAEASIQSAAAALVQAKLDVEHAYVKAPISGRVGRAEIQVG
                     NLVQTSPAAPPVLTTIVSDDGIYADFDVDETTYLKNVRTHATTASQEQKLPVELTVQG
                     D"
     gene            938..1576
                     /locus_tag="VG274_01755"
     CDS             938..1576
                     /locus_tag="VG274_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWE05401.1"
                     /translation="MRPGRPRSFCTDTALDRAMTVFWRQGYEGASLADLTAAMGINPP
                     SLYAAFGSKEGLFRAVLERYDERRKGFMDKVLAAPDAKSVAQLFLEGVAEFASDTSGR
                     NPPGCLLLQSGLSCGDQVIPEALAQHRAEKERALRERFARARKEGDLSSSSDPAALAR
                     YLVAMANGISVQAAAGASVGELREIAHMALAAWPVDGVKGIRKSPRTRQLVH"
BASE COUNT          326 a          564 c          609 g          328 t
ORIGIN      
        1 gtcgccctgc acggtcagct cgaccgggag cttctgttcc tgcgacgcgg tcgtcgcatg
       61 cgtgcgcacg ttcttgagat aggtcgtctc gtcgacatcg aaatcggcgt agatgccgtc
      121 gtcggagacg atggtggtga gaaccggcgg cgctgcgggc gaggtctgca cgagattgcc
      181 gacctggatt tccgcgcggc ccacgcggcc ggagatcggc gctttgacgt aggcgtgttc
      241 gacatcgagt ttcgcctgca ccagcgcggc cgcggccgac tggatggacg cctccgccac
      301 cgcgctcgca ttggcgcgct ggtcgtagag gtcgcgcgcc accgcctgcg cgtccatcag
      361 ccgcttcgcg cgctcgagtt ccgtgcgggc gagccgtgca ttggtcacgc ccgttgccag
      421 gtccgcctgc gccttggcga cggccgcttc ataggtgcgc ggatcgatga cgaggagaat
      481 gtcgcccgcg tgtacctctt gcccatcatg gaaccggatc tcggtgatgc ggccgctgac
      541 ttcggggcgc acgtcggccg aatcgaccgc cgtcatgcgg cccgaatact ccgaccacag
      601 gcgcaccggc ttcgcgccca ccgcggcaac cgtgacaggg atcggagccg gagcggcagg
      661 agcgggcgcg ccgcccgcat ggctgtgcca gacggcaaag ccgaggccgg ccgtgacgac
      721 caatgccgcg acacccagat aagcgcgcgt tttcagggac ctggtgctgc ttaagggctt
      781 ggagccggtc tcgaatttcc cggaattgac cagttcggcg atatgagtca tggccctctc
      841 cataattctg tatcgaacga tacagatagg tactggacga ggaccgtcaa gcctcttatg
      901 tatcatccaa tacaaaaata aaagggagga atgaaaaatg aggccgggcc gtccccggag
      961 cttttgcacc gacaccgcgc tcgaccgcgc gatgacggtg ttctggcgcc agggttacga
     1021 aggcgcctcg ctcgccgatt tgaccgcggc aatgggcatc aatccgccca gcctgtatgc
     1081 cgctttcggc agcaaggagg gtctcttccg cgccgtgctc gaacgctacg acgaacggcg
     1141 aaaagggttc atggacaaag tgctcgccgc gcccgatgca aaatcggtgg cacagctatt
     1201 cctggaaggc gtagctgaat ttgcctccga cacgagcggg cgaaatcctc ccggctgtct
     1261 tctgctgcag agcgggcttt cctgcggcga ccaggtcatt cccgaagcgc tggcgcagca
     1321 tcgcgccgaa aaggagaggg cgctgcgcga gcggttcgcg cgcgcaagga aggaaggcga
     1381 cctgtcctcg tcgtcagacc ctgctgcgct cgcccgctat ctcgtggcga tggcgaacgg
     1441 catcagcgtg caggctgccg cgggcgccag cgtcggggaa ctgcgcgaaa ttgcgcacat
     1501 ggcgcttgcc gcgtggccgg tcgatggggt aaaggggatt cgcaaatcgc cgcggacgcg
     1561 gcagttggtg cattgaagga tttgcatgcg catgacagtc gtgcgcgtcg cgcatgcgca
     1621 acggcccttg aggccgtaac gatcaataca aaagtggggc attggaaaga ggatcgggcg
     1681 gaccgcatgg ggatgcggac gcaatcgagg atgcaatgat cgattcgtct ctgtccacgt
     1741 ggtttgcgct catcatcgcg ggcatgttcg cggcaagcgc tttcgcgcac ctgcttggcc
     1801 cgcgcttcat cctcaaggcc tatgcgc
//
LOCUS       DASZMC010000094         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1143747,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000094 DASZMC010000000
VERSION     DASZMC010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143747"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1194)
                     /locus_tag="VG274_01760"
     CDS             complement(67..1194)
                     /locus_tag="VG274_01760"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HWE05402.1"
                     /translation="MSWSKGEPVEGQRKNLPIAWRLALSTQCIRRERVVENPVLVEIT
                     RSGRVESIHRGAAAISDAAGDVLFSLGDIDVPAYPRSSLKPIQALPLVESGAADAFGL
                     GGEEIALACASHSGEPMHTDRVASWLARLGLGESDLACGPQPPRYEPVWEDMVRRGEK
                     PSRIHNNCSGKHAGFLTLAKHWNISAAGYERRDHPVQQAVAKTLGELAGIAGELPWGV
                     DGCAAPNFAIPLVAFARALAQLATPGALSAPRAPASVRIVAAMMAHPELVAGTARVCT
                     ALMRAGKGRVAVKTGAEGVYAAIVPAHGLGIALKIDDGAGRAAETAISFLLDRMGLLP
                     DRDMVREIIVAPVLNTRDAIVGERRPAAALLEAPLPVLTQP"
     gene            1308..2060
                     /locus_tag="VG274_01765"
     CDS             1308..2060
                     /locus_tag="VG274_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE05403.1"
                     /translation="MTPALSISHLRKVYRRGTVGIEELSLDVAPGEFFGFLGPNGAGK
                     TTTIHCVTGIARPTSGRIEVFGVDAVRNYREARRLVGLSPQEFNVDPFANARDIVGWM
                     GGYYGMKAEERRARTEMLLEKFDLAVHAKKSFRELSGGLKRRVMLARAMINDPKLLIL
                     DEPTAGVDVELRRDLWRYLQEMNRDGKTIFLTSHYLEEVERLCRNIAIISGGRIVRQG
                     PKEEFVNEPGGLEAAYLRATGAEPNHVPESAA"
     gene            2057..>2606
                     /locus_tag="VG274_01770"
     CDS             2057..>2606
                     /locus_tag="VG274_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWE05404.1"
                     /translation="MSTKSPVINWIGLWTIIRREWTRLMRVPIQAFLAPWISALLFIF
                     IFGFVVGGRISQIGGHRYIQFVLPGILMLNIVNASFLQSSSQVYFSRFLRYVEETLVA
                     PLSYAEMIAGVIAVVVVRSVITALGILVIAAFFGATSIDGVAEFVFWIVSVSMVFGLL
                     GIVIGLWANNFEQLTILNIFFIT"
BASE COUNT          444 a          829 c          836 g          497 t
ORIGIN      
        1 tatgcagata cgtgcggtga aatttgttca ggcgcgggtg aggggtcgac gtgctttgtg
       61 tagttgtcag ggctgcgtga gcactggcag cggcgcttcg agcagagccg ctgcggggcg
      121 gcgttcaccg acgatcgcgt cgcgcgtgtt gagcacgggc gcgacaatga tttcacgcac
      181 catgtcgcga tcggggagca agcccatcct gtcgagcaga aatgagatcg ccgtttccgc
      241 ggcgcggccg gcgccgtcgt cgatcttgag cgcaatgccg agcccatgtg cgggaacaat
      301 cgcggcgtag acgccttccg caccggtctt gactgcaacg cggccctttc cggcgcgcat
      361 cagcgcggtg cagacgcgcg cggttccggc cacgagttcc gggtgcgcca tcatcgctgc
      421 gacgatacga accgaggccg gcgccctcgg cgcggaaagc gcgcccggcg tcgccaattg
      481 cgccagcgcg cgcgcaaatg caacgagagg gatggcaaaa ttcggcgccg cgcaaccatc
      541 gacaccccat gggagttcgc cggcaatgcc ggccagttcg ccgagcgttt tcgcgaccgc
      601 ctgctgcacg ggatgatcac ggcgctcgta gccggcagca gaaatgttcc agtgctttgc
      661 cagcgtgaga aatcccgcat gcttgcccga gcagttgttg tggatgcgcg acggtttctc
      721 gccgcggcgg accatgtcct cccagacggg ttcgtagcgc ggaggctgcg ggccgcaggc
      781 gaggtcgctt tcaccaagac ccaatcgcgc cagccatgac gcgacacggt ccgtatgcat
      841 cggctcgccg gaatgggacg cgcaggccag cgcaatctcc tccccgccga gtccgaacgc
      901 gtcggccgcg cccgattcca ccagcggcag agcctggatc ggcttgagcg aggagcgggg
      961 ataggcgggg acgtcgatgt cccccagcga gaacagcacg tctcccgctg cgtcggagat
     1021 tgcggccgcg ccgcgatgaa tgctttccac gcgcccgctg cgggtgatct cgacaagtac
     1081 cggattttcc acgactcgct cccgccggat gcactgcgta cttaacgcaa gccgccaggc
     1141 gatgggcaga ttttttctct gcccttcgac gggctcaccc ttcgaccagc tcacccttcg
     1201 accagctcag ggtgaggaaa tatcccttat gctgagcctg tcgaagcatg agcctgtcga
     1261 agcgtgagct tgtcgaagca tgcggattcg gactagcacg tcgccccatg acgccagccc
     1321 tctcgatctc gcatctgcgc aaggtgtatc gccgcggcac ggtcggcatc gaggagctct
     1381 cgctcgacgt ggcgccgggg gagttcttcg ggttcctcgg ccccaatggc gcgggcaaga
     1441 ccacgaccat tcattgcgtg acgggtatcg cgcggcccac ctccggccgg atcgaagtgt
     1501 tcggcgtcga tgctgtgagg aattatcgcg aagcacgacg actcgtcggg ctctccccgc
     1561 aggaattcaa cgtcgatccg ttcgccaatg cgcgggacat cgtcggctgg atgggtggtt
     1621 attacggcat gaaggcggag gagcgccgcg cgcgcaccga gatgctgctg gagaaattcg
     1681 atctcgccgt ccatgcgaag aagagtttcc gcgaactctc cggcgggctc aagcgccgcg
     1741 tcatgctggc acgcgccatg atcaacgatc cgaaacttct gatcctcgac gagccaaccg
     1801 ccggcgtcga tgtcgaattg cgccgggatc tgtggcgcta cctgcaggag atgaatcgcg
     1861 acggaaagac gatcttcctg acgtcccact atctcgagga agtggagcgg ctgtgccgca
     1921 acatcgcgat catttcgggc gggcgcatcg tgcgccaggg cccgaaggag gaattcgtca
     1981 acgagccggg cgggctcgaa gccgcctatc tgcgggcgac cggtgcggag cccaaccacg
     2041 tgccggaatc cgccgcatga gtacgaaatc gccggtgatc aactggatcg ggctgtggac
     2101 gatcatccgc cgcgaatgga cgcggctgat gcgggtgccg atccaggcct tcctcgcgcc
     2161 ctggatctcg gcgctgctct tcatcttcat cttcgggttt gtcgtcggcg ggcgaatttc
     2221 ccagatcggc gggcaccgct acatccagtt cgtgctgccc ggcatcctga tgttgaacat
     2281 cgtcaatgcg tctttcctgc aatcctcctc gcaggtctat ttctcgcgct tcctgcgcta
     2341 cgtcgaggag acgctcgttg cgccgctctc ctatgcggaa atgatcgccg gcgtcattgc
     2401 cgtggtcgtc gtgcgctccg tcatcacggc gctcgggatt ctcgtcatcg cagccttctt
     2461 cggcgcgacg tcgatcgacg gcgtggcgga attcgtgttc tggatcgttt cggtctcgat
     2521 ggtgttcggg ctgctcggca tcgtcatcgg gctgtgggcc aacaatttcg aacagctgac
     2581 catcctgaac atcttcttca tcacgc
//
LOCUS       DASZMC010000095         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3613565,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000095 DASZMC010000000
VERSION     DASZMC010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3613565"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VG274_01775"
     CDS             complement(<1..1081)
                     /locus_tag="VG274_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWE05405.1"
                     /translation="MIFHVLRALFVLLMAAVGWFYVTHPGPGGGGWAAMPTAIAISVL
                     FIAIDIVSPRRKLAIFAGSFFGLLVGIAIAYALSFVVGLLVDNYYQPGDLKPVDVLAF
                     NQHREAIKSFVDMLIGGVSCYLAISFILQTKDDFRFIVPYVEFSKQTKGARPMLLDTS
                     VLIDGRITDIAETGILDSQLVVPHFVLDELQAVADSADRMKRNRGRRGLDMLAALRKV
                     TKADVVLYETSDLEEGSVDQKLMHLARSLNARVLTNDYNLNKVAQLTGVDVININDLA
                     TAMRPVVVPGEKMRVVVVKPGEEAGQGVGYLEDGTMVVIEQARPFVDEEVEFLVTRAL
                     QTSAGRMIFGRMATESAPSSSPSRPN"
     gene            complement(1201..1689)
                     /locus_tag="VG274_01780"
     CDS             complement(1201..1689)
                     /locus_tag="VG274_01780"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HWE05406.1"
                     /translation="MTLPPSSIDLRPHELSVALLQPQIAPNAGNIGRLCVATGTRLHL
                     VRPMGFVLSDKALRRSAMDYWPRLQVQVHDDTDAFVQAMAGRRIWLFTSKAKRSLWEA
                     DFADGDSLWFGSETHGLPAALIERHAGQSIRIPQSRGERCLNVSTAVGIGLYEALRQI
                     RG"
     gene            complement(1803..>2061)
                     /locus_tag="VG274_01785"
     CDS             complement(1803..>2061)
                     /locus_tag="VG274_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05407.1"
                     /translation="LLWGRFTEAQLLGKKRWSRTGYFGHTPVVNYSAGAGLVPVRGHR
                     IVLLDTASALMPEGMLSAMCAESGRVIQADRLGQTIELPDH"
BASE COUNT          394 a          664 c          635 g          368 t
ORIGIN      
        1 gattcggtcg cgaagggctg gaagacggcg cgctctcggt agccatgcgg ccgaaaatca
       61 ttcgcccggc actggtctgc agcgcgcggg tcaccagaaa ctcgacttcc tcatcgacaa
      121 aaggccgggc ctgctcgatg acgaccatgg ttccgtcttc gaggtagccg acgccctggc
      181 cggcttcctc gcccggcttg acgacgacca cccgcatctt ctcgccgggc accacgaccg
      241 gccgcattgc ggtggcgagg tcgttgatgt tgatcacgtc caccccggtg agctgcgcga
      301 ccttgttgag gttgtagtcg ttggtgagca cgcgcgcgtt gaggctgcgg gccaggtgca
      361 tcagcttctg atcgacgctg ccctcctcca ggtccgaggt ttcgtagagc acgacgtcgg
      421 ccttggtgac tttgcgcagg gctgccagca tgtccaggcc tctgcgcccg cgattccgct
      481 tcatccggtc ggcactgtcg gccaccgcct gcagctcgtc gagcacgaag tgcggcacca
      541 ccagctggct gtccaggatc ccggtctcgg cgatgtcggt gatccggcca tcgatcaaca
      601 cgctcgtgtc cagcagcatg ggccgggcgc ctttggtctg cttgctgaac tcgacgtacg
      661 ggacgatgaa ccggaaatcg tccttggtct ggaggatgaa gctgatcgcc aggtaacaac
      721 tcaccccgcc gatcaacatg tcgacaaagc tcttgatcgc ctcgcgatgc tggttgaatg
      781 ccagcacatc caccggcttg aggtctccgg gttgatagta gttgtccacc aagaggccca
      841 cgacgaagct gagggcatac gcgatggcaa tgccgacgag aaggccgaaa aacgacccgg
      901 caaagatagc gagcttccgc cgcggcgaga cgatatcgat ggcgataaac agaacggaga
      961 tggcgatggc ggtcggcatc gccgcccagc ctccgccgcc gggccccggg tgggtgacgt
     1021 agaaccatcc cacggcagcc atcagcaaga caaacagtgc ccgcaggacg tgaaaaatca
     1081 tggtctggtt cccacaaaga gaggcggcaa caaaatatag ttcatgcccg ccatggcgtc
     1141 caacggattg tgcgggcgcg gtgatccttt ccacgtttgg gaattccgtc cggtcgcgca
     1201 tcagccgcga atctgccgca gggcctcata tagaccgatc ccaactgccg tggagacatt
     1261 caggcaccgc tcgccgcgcg attgcggaat tcggatggat tgcccggcat ggcgctcgat
     1321 cagcgccgca ggcagcccgt gtgtttcgct gccgaaccac aggctgtcac cgtccgcgaa
     1381 atcggcttcc cacagcgagc gctttgcctt gctcgtgaaa agccagatgc gccggccggc
     1441 catggcttgc acgaacgcat cagtgtcatc gtggacctga acctgcagtc gcggccagta
     1501 gtccatcgcg ctgcggcgca gcgccttgtc cgacagcaca aatcccatgg gccgaaccag
     1561 gtgcagccgc gtccccgtgg cgacgcacag ccggccgatg ttcccggcat tgggcgcgat
     1621 ttgcggctga agcagcgcca cgctgagctc atggggccga aggtcgatcg atgaaggggg
     1681 aagcgtcatg aacctcggaa ccgcgaaatg tccggtcaat ttacttgccg ccaatcagcc
     1741 agtcccggcg cggcttccgg cagcgccttt cacttgctct caccacctgc ggacacgcgg
     1801 ctctaatgat ccggcaattc aatcgtctgg ccgagccgat cagcctggat gacccgcccg
     1861 ctttcggcgc acatggccga gagcatcccc tctggcatga gcgccgatgc ggtgtcgagc
     1921 aggacaattc gatgtccgcg aacggggacg aggccggcgc cggcggaata attcaccacg
     1981 ggcgtgtgcc cgaaatatcc ggttcggctc caacgcttct tgccgagaag ttgcgcctcg
     2041 gtgaatcgac cccaaagcag c
//
LOCUS       DASZMC010000096         5876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4184411,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000096 DASZMC010000000
VERSION     DASZMC010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4184411"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VG274_01790"
     CDS             complement(<1..753)
                     /locus_tag="VG274_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE05408.1"
                     /translation="MHVPTSLRSGSAGDALVLLHGIGGRAQVWQPQLDAFSSRFCVVA
                     WNMPGYGGSEPLPQITFPAIAEALEALLDTLPGKRIHLVGHSMGGMVAQEFLRRRDHR
                     VTSVCLYATSPGLAQPETPEALQAARERAREFMARRIGPLDAGKTMRDMAESLLDQLL
                     SPRAPAAARAAAVESISSVPPAVYRDAMRCILSFDGTAIVPKIDRPTLVIAGGEDRTI
                     GPTIVEAMARQIVGARFEIIPGVGHLANLEDPP"
     gene            complement(830..3742)
                     /locus_tag="VG274_01795"
     CDS             complement(830..3742)
                     /locus_tag="VG274_01795"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HWE05409.1"
                     /translation="MNERESVDAFANHTADLPRPFDPTRAHRTFEELGETVCGIPHAA
                     RECFASAFANSPYLARLALRERLALRGLWERGPGTLFEAAAHDAELVHEAEQSAAAMA
                     ILRRAKRQAALAIALADISGLWNLDATMRALTRFADASAGGALRFLLREAAASAGIAE
                     RDPAVLEAETGLVVLAMGKAGAFELNYSSDIDLVVFYDADRLPFRKRSDARAAAVDLT
                     KGLVKLLGEITADGYVFRVDLRLRPDAGATQIAISTEAAERYYEEMGQNWERAAFIKA
                     RPCAGDRSAARMFLKRLEPFVWRRNLDYAAIEDIHSIKRQIHAHGRHGPIAVAGHNLK
                     LGRGGIREIEFFAQTQQLILGGRDPSLRQSGTLDAIAALAARGLVSESASADLADSYC
                     FLRGLEHRLQMIDDEQTHTLPRTAEGLAHIACFAGFADTQAFSEKLVVRLRSVQAHYD
                     GLFEKAPPLGGAGGSLVFTGVEDDPETIDTLREMGFRDPRHAAGAVRGWHHGRIRATR
                     SARARELLTRLVPALLDALAATADPDAAFAQFDRFVSRLPAGVQLFSLFLANPQLLRL
                     VASIAGSAPRLADHLGRSPAMLDAVLDRGFLAELPSREALAALLQEGLAAATDHESVL
                     DAVRRYAKEQMFRVGVHLIDGRATPENAGLTFSNIAECVIAGLLERVERELEATSGVV
                     KGGAFCVVAMGKLGGREMTASSDLDLVFVYDAPATADRSSGDRSLPLMVYYARLAQRL
                     ISALTVPTAEGVLYEVDMRLRPSGNKGPAAVSLGSFVRYHAQEAWTWEKLALTRARVV
                     AGPAALRKNIEDAILNVLTERIEPVALIRDAREMRGKLRAQFPGRDRWDVKFRRGGLV
                     DIEFIVQTLQLRHACDLPRILEQNTVVALESLAAAKLLGAEEAQTLVGAARLQLALLQ
                     VLRISVAGAFDPVFATAGMKALLSRAGGMGDFGEIEDRLEAAQIEVHALFERLLTA"
     gene            complement(3808..4092)
                     /locus_tag="VG274_01800"
     CDS             complement(3808..4092)
                     /locus_tag="VG274_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWE05410.1"
                     /translation="MSKADFIETVASKGNITKADAKRYVELVFDSIEAGLKTARSSGK
                     LHIRTFGTFHVSKRRARIGRNPKTGESIKIKASRSLRFKPASQLKEAAGC"
     gene            complement(4142..5566)
                     /locus_tag="VG274_01805"
     CDS             complement(4142..5566)
                     /locus_tag="VG274_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWE05411.1"
                     /translation="MRAPRIFRTLAFRIVVVYLAIFAVSAAAIVAFTYWNTARALNAQ
                     TDQIVEADITGLSERYDRFGLAGLTDAIVNRSSHGGATLYVLADSDKRPIAGNLDAWP
                     ELAQIGGNLVEFDYQRRLGNRVEQRRARGRDFDLTGGFELLVARDIAERHETEKLFTT
                     MIPWSVLLIVVLGLVGGGLISRNFLARLDQINRTSRVIMAGDLSRRLPVGHAGDEFDV
                     LALNLNRMLDRTERLMRGMREVTDSIAHDLRTPLNRLRNRLEGTLHSLDSHGAAGAQI
                     EAAVEETDRLMATFNALLLIAEAEAGVAREAMTPVDLRGIVEGVVELYAPLADEKGVS
                     LSAAPSGQAIVNGIGSLVSQALANLLDNAIKYTPAGGRVNISVEDTPGGIALCVADTG
                     PGIASEDRNRVLERFVRLEASRHSPGTGLGLSLVAAVARLHDAKLELEDNGPGLKATL
                     LFPAGANCRSSVPQIAPQKMAVRT"
     gene            complement(5553..>5876)
                     /locus_tag="VG274_01810"
     CDS             complement(5553..>5876)
                     /locus_tag="VG274_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWE05412.1"
                     /translation="RSPVTVKTRLQVGDLDLDLLTRAAKRAEQPIDLQPREFRLLEYL
                     MRHAGQVVTRTMLLESVWEYHFDPQTNVIDVHISRLRAKIDRGFEVPLLHTVRGAGYM
                     IRARA"
BASE COUNT         1071 a         1912 c         1870 g         1023 t
ORIGIN      
        1 gggcgggtcc tccagattgg cgaggtggcc gacgccggga atgatctcga accgggcgcc
       61 cacgatctgc cgcgccatgg cctcgacgat cgtcggtcca atcgtgcggt cctcgcctcc
      121 agcaatgacg agcgtgggac gatctatctt cggcacgatt gcagtgccat cgaatgagag
      181 gatgcagcgc atcgcgtcgc gatagacggc gggcgggacc gagctgatcg attcgaccgc
      241 agccgcacgc gccgctgcgg gcgcacgcgg cgacagcagc tgatcgagca gactttcggc
      301 catgtcgcgc atggtctttc cggcgtcgag cggcccgatc cggcgcgcca tgaattcgcg
      361 cgcccgttcg cgcgccgcct gcagcgcttc gggcgtttcg ggctgggcca ggccgggact
      421 cgtcgcatag aggcaaacgc tcgtgaccct gtggtcgcgg cgccgcagga attcctgcgc
      481 caccatgccg cccatcgagt ggccgaccag atgaatgcgt ttgccgggaa gcgtatcgag
      541 cagcgcctcg agcgcttcgg caatcgccgg gaatgttatt tgcggcaacg gctcggatcc
      601 gccatatccc ggcatgttcc aggcaacgac gcagaagcgc gaagaaaacg catcgagctg
      661 cggctgccag acctgcgcgc ggccgccaat gccgtgaaga aggacgagcg cgtcgcccgc
      721 cgacccgctt ctcagggatg taggcacgtg catcgctcgt tctccgcttg atcggcaccg
      781 gcccgttgaa agcttacgac atcgttcgtc gcaaacgagc acccaatcgt cacgccgtca
      841 gcagacgttc gaacagggcg tggacctcaa tttgcgccgc ttcgagacga tcctcgattt
      901 cgccgaagtc gcccataccg cctgcgcgcg agagcagcgc cttcattcct gcggttgcga
      961 agacgggatc gaaggcgccg gcgaccgaaa tccgcagcac ctgaagcaac gccagctgca
     1021 aacgcgcggc gccgaccagc gtttgcgctt cttccgcgcc gagcagcttc gccgcagcga
     1081 ggctttcgag cgcaaccacg gtgttttgct cgagtatccg ggggaggtcg caggcgtggc
     1141 ggagctggag cgtctggacg atgaattcaa tgtcgaccag cccgccgcgc cggaatttaa
     1201 cgtcccaccg atcccggccg gggaattgcg cccggagctt cccgcgcatt tcgcgtgcgt
     1261 cgcggatgag cgccaccggc tcgattcgtt cggtcagcac attcagaatc gcatcctcga
     1321 tgttcttgcg taatgcggcg ggtcccgcaa caacccgggc gcgcgtcagc gccagtttct
     1381 cccaggtcca cgcttcttgg gcgtgatatc ggacgaagga acccaggctc acggccgcgg
     1441 gacccttgtt gccgctcggc cgcagtctca tgtccacttc gtagaggaca ccttccgccg
     1501 tcggcaccgt cagcgcggag atcagcctct gtgcgagccg cgcgtaatag accataagcg
     1561 gcagggagcg atccccgctc gaacgatctg cggtggcggg cgcgtcatag acgaatacaa
     1621 gatcgagatc ggatgacgcc gtcatctcgc ggccgccgag cttgcccatc gcgacgacgc
     1681 agaacgcgcc gcctttcaca acccctgaag ttgcttcgag ttcgcgttcc acccgttcga
     1741 gaagacctgc aatgacgcac tcggcgatgt tcgaaaacgt caggccggca ttttcaggcg
     1801 tcgccctgcc gtcgatgaga tgcacgccga cccggaacat ctgttccttc gcgtagcgcc
     1861 tcaccgcgtc cagtacggat tcgtgatccg tcgccgcggc gagtccctcc tgcagcaggg
     1921 cggcgagggc ttcgcgcgag gggagctctg cgaggaagcc gcgatcgagt acggcatcga
     1981 gcatcgccgg tgaccggccg agatgatcgg caagccttgg tgcggagccc gcgatggagg
     2041 cgacgagccg cagcaattga ggattggcga gaaacagcga gaagagctgg acgcccgccg
     2101 gcaaacgcga cacgaagcga tcgaactggg caaacgcggc atcgggatcg gcggtcgcgg
     2161 caagcgcgtc gaggagcgcc ggcaccagcc gggtcagcag ttcgcgcgcg cgcgcgcttc
     2221 gagtggcgcg gatgcggccg tgatgccagc ctcgcacagc tccagcggca tgccggggat
     2281 cgcgaaaacc catctcgcga agcgtgtcga tcgtttcggg gtcgtcctcg accccggtga
     2341 acaccagact ccctccagcg ccgccgagcg gcggcgcttt ctcgaaaagg ccgtcatagt
     2401 gagcctgaac gctccggaga cgtaccacca gcttttcgct gaacgcttgc gtgtccgcaa
     2461 agcccgcgaa acaggcgata tgggccaggc cctccgcggt cctgggcaag gtatgggtct
     2521 gctcgtcgtc gatcatctgc aggcgatgct cgagcccgcg caggaaacaa taggagtccg
     2581 cgagatcagc gctcgccgat tccgaaacga gccctcgggc cgcaagcgcg gcgatcgcat
     2641 cgagggttcc agactgcctg agcgaaggat cgcgcccgcc cagaatcaat tgctgggtct
     2701 gggcgaagaa ttcgatctcg cggatgccgc cccggccaag cttcagattg tgtcccgcga
     2761 ctgcaatcgg gccatgtcgg ccgtgggcgt gaatctggcg cttgatcgaa tggatgtcct
     2821 cgatcgcggc ataatcgaga ttgcggcgcc acacgaacgg ctcgagtcgc ttgagaaaca
     2881 tccgcgccgc agaacggtcg ccggcgcagg ggcgcgcctt gatgaacgct gcgcgttccc
     2941 agttctgtcc catctcctcg taataccgct ctgccgcttc ggtcgagatg gcgatctgcg
     3001 ttgcgcccgc gtcgggtctc aatctcaaat cgacacggaa gacgtagcca tcggcggtaa
     3061 tctcgccaag cagcttcacc agccccttgg tgagatcgac cgccgcggcg cgcgcatcgg
     3121 acctcttgcg aaaaggaagg cgatcggcgt cgtagaagac gaccaggtcg atgtcgctgg
     3181 agtagttcag ctcgaacgcg ccggcttttc ccatcgccag aacgacgaga cccgtctccg
     3241 cctcaagaac cgccggatcg cgctccgcaa ttcccgcgga cgcggccgcc tcgcgcaaca
     3301 ggaagcgaag cgctcctcct gcgcttgcat cggcaaatcg tgtgagcgcg cgcatggtag
     3361 cgtcgagatt ccacaggccc gatatgtccg cgagcgcgat tgccagcgca gcctgccgct
     3421 tggcgcggcg caggatggcc attgcggcgg cactttgctc cgcctcgtgc accagctccg
     3481 cgtcgtgcgc cgccgcttcg aacagcgtcc cgggcccccg ttcccaaagc ccgcgcagag
     3541 cgagccgttc gcgcaacgca agacgcgcga gatagggact attcgcgaac gcgctcgcga
     3601 agcattcgcg tgcggcatgc gggataccgc aaaccgtttc gcccagctct tcgaacgttc
     3661 ggtgggccct tgtgggatcg aagggtcgtg gcagatcagc tgtgtgattc gcaaacgcat
     3721 ccacgctttc gcgttcgttc acgccggcac acgtgcgcaa gagcccgcgg gagcgagcgg
     3781 cgtcttcccg cccgcggggg aagatgatca gcagcccgcg gcctctttca actgcgacgc
     3841 gggtttgaaa cgcaggcttc tggatgcctt gatcttgatc gattcgcccg tcttcgggtt
     3901 gcgtccgatg cgcgcccggc gtttggaaac atgaaacgtt ccgaatgtcc tgatatgaag
     3961 cttgccgctg ctccttgccg tcttgagccc ggcctcgatc gaatcgaaga caagctccac
     4021 gtaacgtttc gcatcggcct tggtgatatt tcccttgctg gccacggtct cgatgaaatc
     4081 ggctttgctc atcttggcgt cccttcggct ctcgacggtg attccgcaaa tatagccgaa
     4141 ttcaggtccg caccgccatt ttctgcggcg cgatctgcgg aacggaagac cggcagtttg
     4201 cgccggcggg aaaaagaagc gtcgccttca atcccggccc gttgtcttcg agctcgagct
     4261 ttgcgtcgtg caggcgggcg actgcggcga caaggctgag gcccagtccc gttcccggcg
     4321 aatgccggct tgcctccagc ctcacgaaac gttcgagaac gcggttgcgg tcttcggacg
     4381 caattcccgg acccgtatcg gcaacgcaaa gcgcgatacc gccgggcgtg tcttcgaccg
     4441 aaatgttcac gcgcccaccg gccggagtgt atttgattgc attgtcgaga agatttgcga
     4501 gcgcctggct gaccagactg ccaatgccgt tgacgatcgc ctgtccgctg ggcgcggccg
     4561 acagcgagac gcccttctca tccgccagcg gggcgtaaag ctcgacgaca ccttccacga
     4621 tgccgcgcag atcgaccggc gtcatcgcct cgcgggcaac gccggcttcg gcctccgcaa
     4681 tcaggagtag cgcattgaat gtcgccatga ggcggtccgt ctcctcgaca gcggcctcga
     4741 tctgcgcgcc cgccgcgccg tgggaatcga gcgaatgaag cgtgccttcc agacggttgc
     4801 gcaatcgatt gagcggcgtg cgcagatcgt gggcgatcga atcggtgact tcgcgcatac
     4861 cgcgcatcag ccgttctgtg cggtccagca tgcgattgag attcagggcg agaacgtcga
     4921 attcgtcccc ggcatgtcca accggcaggc ggcgcgaaag atccccggcc atgatcacgc
     4981 ggctggtgcg gttgatctga tccagccgcg ccaggaaatt ccggctgatc aggccgccgc
     5041 cgaccagccc gagcaccacg atcagcagca cactccaggg aatcatggtc gtgaacagct
     5101 tctcggtttc gtggcgttcg gcgatgtcgc gcgcgacgag aagctcgaat ccgccggtca
     5161 ggtcgaaatc gcgtccgcgc gcacggcgct gttcgacacg gttgccgagc cgccgctgat
     5221 aatcgaattc gaccaggttg ccgccaatct gggcgagttc cggccatgca tccagattgc
     5281 cggcgatcgg ccgcttgtcc gaatcggcca gaacataaag ggtggcgccg ccatgcgacg
     5341 agcggttgac gatcgcgtcc gtcagtcccg ccaggccgaa tcggtcatag cgctccgaca
     5401 gtccggtgat atccgcttcg acaatctgat cggtctgcgc gttcagggcg cgcgccgtgt
     5461 tccaatacgt gaaggcgacg atggccgccg cggacaccgc aaatatggcg agataaacga
     5521 cgacgatgcg aaacgcgaga gtgcggaaaa tcctaggcgc gcgcacggat catatagccc
     5581 gcaccgcgca cggtgtgcag cagcggaacc tcgaaccccc tgtcgatctt cgcgcgcagc
     5641 cgggagatgt gcacgtcgat cacgttggtt tgcggatcga aatgatactc ccagacgctc
     5701 tcaaggagca tcgtgcgcgt gacgacctgg ccggcatgac gcatcagata ttccagcagg
     5761 cggaattcgc gcggctgaag gtctatcggc tgttcggcgc gcttggcggc gcgggtgaga
     5821 agatccaggt ccagatcgcc gacctgaagg cgcgtcttca ccgtcaccgg cgagcg
//
LOCUS       DASZMC010000097         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2801930,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000097 DASZMC010000000
VERSION     DASZMC010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801930"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="VG274_01815"
     CDS             <1..1525
                     /locus_tag="VG274_01815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HWE05413.1"
                     /translation="ARDRRAHKMRFERGKAVGKLKDLGAVNRRGTIVSFHPDPQIFGK
                     DTHFSAARLYRMVKSKAYLFRGVEIRWKCDPSLIKGETPAEDVLKFPGGLLDFLNSEI
                     GKNRTVTQRAFSGRTENRDGKGAVEWAVVWTPQIDPFVHSYCNTIPTSQGGTHEQGLR
                     NALTKALRTHGESNRKTALITTDDVMSGACAVLSVFIREPEFQGQTKDKLSTPDAQRL
                     VENVVKDHFDHWLAENPQEADKLLEFVIDQAEDRLKKRQEKETQRKSATRKLRLPGKL
                     ADCSRDAAAGTEIFLVEGDSAGGSAKQARDRATQAILPLRGKILNVASAGAGKLAQNQ
                     ELTDLLQALGCGTGAKYDDNALRYERVIIMTDADVDGAHIASLLLTFFYREMPKLIAN
                     AHLFLAMPPLFRLSHGGTIVYARDDADRERILKTQFKSNAKVEVSRFKGLGEMMPAQL
                     KETTMRPGHRTLVRVEIADSEQANTESLVERLMGKKPELRFQFIQENAQFAAGAVDI"
     gene            complement(1623..>2879)
                     /locus_tag="VG274_01820"
     CDS             complement(1623..>2879)
                     /locus_tag="VG274_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05414.1"
                     /translation="VLMIGATFRARLPEARSRDWMYALLMPPVLLIVCVVIGFAAHLM
                     AQAISGQPDPSYAYAIALREGLGFGVLAAALLAARMAIPQLAAYSVWLWFATLSVVTA
                     LLAPGISPYFLFPTLLAALAGLMVILVPDAPRGRTIWMFVPAVIVALSIWMPLVATGE
                     TLMGLKLHELFTVTAAFAAMTLIPYANVTAMRRSVWLDIFGGSVVGALIATTIAGFQP
                     AYSKTQAQRLSIRYMENAATNKPVWALDADEPLPPSLRAAADFSKTPQTVLPPPFAKN
                     YVAPAGATRFKAPDGKIVSDAVVSGAHRVTIALQGSPQASGMLLIIPKDAALRVIDIH
                     GERVVAPKGNSDDTVLACVSRDCATETVGLEFGSRKDIRLTLVEDRYGAPPFAAKLVA
                     ARPKNAIPSQNGDVVALVNTITVKAK"
BASE COUNT          608 a          919 c          867 g          485 t
ORIGIN      
        1 cgcacgcgac cgccgcgccc acaagatgcg ctttgagcgc ggcaaggccg tcggcaagtt
       61 gaaagacctt ggcgcggtga accggcgcgg taccatcgtg tcgttccatc ccgatccgca
      121 gattttcggc aaggacacgc atttctccgc cgcgcgtctc tatcgcatgg tgaaatcgaa
      181 ggcgtatctg tttcgcggcg tcgagatccg ctggaaatgc gatccgtcgc tcatcaaggg
      241 cgaaacaccg gcggaagacg tcctgaagtt ccccggcggt cttctcgact ttctcaacag
      301 cgaaatcggc aaaaacagaa ccgtcacgca gcgcgcgttt tccggccgca cggaaaaccg
      361 ggatggcaaa ggcgcggtcg aatgggctgt tgtgtggacg ccgcagatcg atccgttcgt
      421 ccattcctat tgcaatacga tcccgacctc gcagggcggc acgcacgagc aagggcttcg
      481 aaatgcactg acgaaagcgc tgcgcacgca cggcgagagc aaccgcaaga ccgcgctcat
      541 cacgaccgat gacgtgatgt ccggcgcatg cgcggtgctg tcggtgttca ttcgcgagcc
      601 ggaattccag ggacagacca aggacaagct ctccacaccg gatgcacagc gcctggtcga
      661 gaacgtggtc aaggatcact tcgaccactg gctcgcggaa aatccgcagg aagcggacaa
      721 gcttctggag tttgtgatcg accaggctga ggaccgcctg aagaagcggc aggaaaagga
      781 gacgcagcgc aaatccgcga cgcgcaagct ccgccttccc ggcaagctcg ccgattgctc
      841 gcgcgatgcg gcggcgggca cggagatttt cctagttgaa ggcgacagcg ccggtggctc
      901 cgccaaacag gcgcgcgatc gcgcgacaca ggcgatcctg cccctgcgcg gcaagatcct
      961 gaacgtcgcg agtgcgggtg ccggcaagct cgcacaaaac caggaactca ccgatctgct
     1021 gcaggcactg ggttgcggga cgggcgcgaa gtatgacgac aacgcgctgc gctatgaacg
     1081 cgtcatcatc atgaccgacg ccgacgtcga cggtgcgcat atcgcgtcgc tgttactcac
     1141 cttcttctat cgcgagatgc ccaagctcat cgcaaacgcg catctttttc tggccatgcc
     1201 gcccctcttc cgccttagcc acggcgggac catcgtctat gcgcgcgacg acgccgaccg
     1261 cgaacgcatc ctgaagacgc aattcaagtc gaacgcaaag gtcgaggtct cacgcttcaa
     1321 aggcctcggc gaaatgatgc cggcgcagct caaggaaacc acgatgcggc ccggtcatcg
     1381 caccctcgtc cgcgtcgaga tcgcggacag cgagcaggcc aacaccgaga gccttgtcga
     1441 gcgcctgatg ggcaagaagc ccgaactgcg cttccagttc atccaggaaa acgcacagtt
     1501 cgcggcgggc gccgtggaca tttagacttc ttttcaattc gtcatggccg ggacaagccc
     1561 ggccatgacg attgcggtgg tgactaggtt cgcaatacaa cggatcgcgg gacgtcatcg
     1621 tcctatttcg ctttcaccgt gatcgtgttc acgagtgcga cgacatcgcc gttctgcgag
     1681 gggatcgcgt tcttcgggcg cgcggccacg agcttcgcgg caaagggcgg cgcgccatag
     1741 cggtcctcga ccagcgtcag cctgatgtct ttgcgcgatc cgaactccag cccgaccgtc
     1801 tcggtcgcgc aatcgcgtga gacgcacgca agaaccgtgt cgtcgctgtt gcctttcggt
     1861 gcaaccacgc gttcgccatg gatgtcgatc acgcgtagcg cagcatcctt cgggatgatc
     1921 agcaacattc ccgatgcttg cggcgaacct tgcaacgcaa tagtgacgcg atgcgcaccg
     1981 cttacaacgg catcggagac gatctttccg tccggcgcct tgaaccgcgt cgcaccggca
     2041 ggcgcgacgt aattcttcgc gaacggtggt ggcaggactg tctgcggcgt tttcgagaaa
     2101 tcagcagcag cgcgcagaga tggcggaagt ggttcgtccg catcgagcgc ccaaaccggt
     2161 ttgttggtcg cggcattctc catatagcga atgctcaggc gttgtgcctg cgtcttgctg
     2221 taggcaggct ggaacccggc gatcgtggtt gcgatcagcg cgccgacgac ggacccgccg
     2281 aaaatatcca gccacacgct gcgccgcatc gcggtgacgt tggcgtatgg gatgagcgtc
     2341 attgccgcaa acgctgccgt caccgtgaac agctcgtgca gcttcaggcc catcagcgtt
     2401 tcacccgtcg cgaccagtgg catccagatc gacagcgcaa cgatgaccgc cggcacgaac
     2461 atccagatcg tccggccgcg cggcgcatcc ggcacaagga tcaccatcaa acccgcgagc
     2521 gccgcgagaa gtgttgggaa cagaaaatat ggacttatgc ctggcgccag gagcgccgta
     2581 accacgctca gagtcgcaaa ccacagccat accgaataag ccgcgagttg tgggatcgcc
     2641 atgcgcgcgg ccagaagcgc ggccgccagc acgccgaatc cgagtccttc gcgcaacgca
     2701 atcgcataag cgtaggacgg atcaggctgc cccgaaatcg cctgcgccat cagatgcgcc
     2761 gcgaagccga tgacaacgca gactatcaac agaaccggcg gcatcagcag cgcatacatc
     2821 cagtcccggc tccgtgcttc cggcagacgc gcacggaacg tcgcgccgat catcagcac
//
LOCUS       DASZMC010000098         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_864458,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000098 DASZMC010000000
VERSION     DASZMC010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864458"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /gene="ccmA"
                     /locus_tag="VG274_01825"
     CDS             <1..646
                     /gene="ccmA"
                     /locus_tag="VG274_01825"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HWE05415.1"
                     /translation="RGASVSVERGESLALLGRNGAGKTTLLRILAGLSKPAEGQALIL
                     GAPARDEAVRRRIGYLGHGIGVYDDLSALENLMLFGRLMEMDDTARAAESALERVGLA
                     TVKDGLAREFSRGMRQRLAIARAFLHEPEVLFLDEPFTSLDDRAIAVLQKILAEALAR
                     GATIVMSTHQIREAMELATNVALLERGKIVHAGARTAEMMADPTWLYRTFGETQ"
     gene            660..1343
                     /locus_tag="VG274_01830"
     CDS             660..1343
                     /locus_tag="VG274_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWE05416.1"
                     /translation="MKRGLVAALSIARKDLKTELRTKESLNASASFALVILVLFSFAF
                     DLDRESIYAISGGLLWLVYSFAGALIVNRSFARELPNDCLDVLVASPTPGWALFLGKA
                     VAAFLLLLIVEAISLPIFGLFYNINWTGVFWPLAGVVALTTWGITVVGTAFSAVTVNV
                     RLRELMLPVLLYPILIPLLIAAMGMTSGLLSGNLTAPDGAQYLRQLIAFGVIYTALSL
                     YMIEFILVV"
     gene            1353..>1577
                     /locus_tag="VG274_01835"
     CDS             1353..>1577
                     /locus_tag="VG274_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05417.1"
                     /translation="MREKAVYACGIAGTALMLWNLHTIFMDLPNDALQGFIFRIIFIH
                     VPAALVADVLYTIALITSILFLSKKKFIYDS"
BASE COUNT          258 a          435 c          544 g          340 t
ORIGIN      
        1 gcgtggcgct tccgtgagcg tggagcgcgg cgaatctctg gcgctattgg gccgcaacgg
       61 cgcggggaag acgactctgc tgcgcattct cgcgggacta tcgaaaccgg ccgaggggca
      121 ggcgctgatt ctcggggcgc cggcgcgcga tgaagccgtg cggcggcgga tcgggtatct
      181 ggggcacgga atcggcgtct acgacgatct ctccgcgctc gaaaatctga tgctcttcgg
      241 gcgcctgatg gagatggacg atacggcgcg agcggccgaa tcggcgctcg agcgggttgg
      301 cctcgcgacc gtgaaagacg gcctcgcgcg ggagttttcg cgcggcatgc ggcagaggct
      361 ggcgattgcg cgcgccttcc tgcacgagcc ggaagttctg tttctggatg agccgttcac
      421 gtcgctcgac gatcgcgcca tcgcggtgct gcagaagatt cttgccgagg ctctcgcgcg
      481 cggcgcgacc atcgtgatgt ccacgcacca gattcgcgag gccatggaac tggcgaccaa
      541 tgttgcgctg ctcgagcgcg gcaaaatcgt gcacgcgggc gcgcgaacgg cggagatgat
      601 ggcggatccg acctggcttt accggacctt cggagagacg cagtgagggc cgccagggcg
      661 tgaagcgcgg acttgtggcg gcgctttcga ttgcgcgcaa ggacctgaag acggagctgc
      721 ggaccaagga atcgctgaac gcgagcgcat cgttcgcgct ggtgatcctg gtgctgttca
      781 gtttcgcctt cgatctggat cgcgagagca tctatgccat ttcgggcggt cttctgtggc
      841 tggtttactc gtttgcgggc gcgctgattg tgaaccgcag ctttgcccgc gaactgccga
      901 acgattgtct ggatgttctg gtcgcctcgc cgacgccggg atgggcgctg tttctgggca
      961 aagcggtggc ggcgtttctg ctgctgctga tcgtggaagc gatctcgctg ccgatcttcg
     1021 gcctgttcta caacatcaac tggacgggcg tgttctggcc gctcgcgggg gtggtggcgc
     1081 tcacgacgtg gggaattacg gttgtgggga cggcgttcag cgcggtgacc gtgaatgtgc
     1141 ggttgcggga actgatgctg ccggtgctgc tttatccgat cctgattccg ctgctgattg
     1201 cggccatggg aatgacgagc ggcctgctga gcggcaacct gaccgcgccc gacggcgcgc
     1261 agtatctgcg gcaactgatt gcgttcggcg tcatatatac cgcgctgtcg ctgtatatga
     1321 tcgagtttat tctggtggta taggacaaaa gaatgcgcga aaaggctgtc tacgcctgcg
     1381 gcatcgccgg aacggcgctc atgctgtgga acctgcacac gattttcatg gacctgccga
     1441 atgatgcgct gcagggcttc atttttcgga ttattttcat ccatgtgcca gcggcgctgg
     1501 tcgcggacgt cctgtacacg atcgcgctga ttacgagcat tcttttctta tcgaagaaaa
     1561 agttcatcta cgattcg
//
LOCUS       DASZMC010000099         3080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4441539,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000099 DASZMC010000000
VERSION     DASZMC010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4441539"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VG274_01840"
     CDS             complement(<1..800)
                     /locus_tag="VG274_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05418.1"
                     /translation="MTGIAAYGAYIPRLRLQRKAAAAANAWFAPGLLASAKGERSMAG
                     WDEDALTMAVEAGRDCLPAADPLRDRAGTDALYFASTSQPYSDRLNAAIVARALSLPE
                     AISAIDVAASQRAGLTALVAGFDALRSGRATRPLIVAGERRKARAASSQELSFGDAGA
                     ALALGRGESIANFLGSHSLTIDFVDHFRGSAEEFDYGWEERWIRDEGFVKIVPVAIRG
                     LLEKTGVNGADIAHFILPCPFPRLDQQLAGRCGIDPARVRDNLAGQVG"
     gene            960..1841
                     /locus_tag="VG274_01845"
     CDS             960..1841
                     /locus_tag="VG274_01845"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HWE05419.1"
                     /translation="MPTPPPVRKAVLPVAGMGTRFLPATKAVPKEMLPVVDKPVVQYA
                     VEEAKEAGIEQFIFVTGRGKYIIEDHFDHAHELESLLSSRDKTAELNSLLEDLPITGS
                     VSFTRQQKPLGLGHAVWSARHFVGNEPFAVLLPDDLLVGKPGAMTRMIEAYHAVGGGM
                     IVAAEDKPLAETGRYGVIEPGATVGDVVEVKGVVEKPAPENAPSRLCIIGRYILGPEI
                     FAELDRQERGAGNEIQLTDSMARLIGRVPFHAVRTGCARYDCGDKVGFLHANIAVGLT
                     RPDIAPALRAILKDVDF"
     gene            1973..>3080
                     /locus_tag="VG274_01850"
     CDS             1973..>3080
                     /locus_tag="VG274_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05420.1"
                     /translation="MGIPAKSVARPMRIGHLLAAGIACLLALGGPALARSSRTQSGEA
                     GGTTYAIRFDHWSDQDERDFGEFIAGIGDSGCTTVDTCLRDPSNPFRASDIGGSFFQA
                     DCAKLMYILRAYFAWKRGLPFSYANGVAPVGGGADARYSPEGNRVTSRAEVLTGSASG
                     YALLNNVLSSVSSANYRMHPDLDSPLRPDFYSVAINAKSVRPGTAMYDPNGHVGIVYK
                     VESDGRIRLIGSHPDNTLTRDYYDLRFVRSRPGVGAGFKNWRPVRLVGYTRRSDGALV
                     GGHMELASNSEIPDFSDEQYYGNGQRPTDEDWASGTFSLNGETVDYYDYVRAKIAGGR
                     LEFDPVKEVADMVDSNCSDLHDRVQAVNIALQAGI"
BASE COUNT          597 a          964 c          980 g          539 t
ORIGIN      
        1 tcgccgacct ggccggcaag attgtcgcgc acacgcgccg gatcgattcc gcagcgcccg
       61 gcgagctgct ggtccaggcg cgggaacggg caaggcaaaa tgaaatgcgc aatgtcggcg
      121 ccgttgacgc cggtcttttc gagcaagccc ctgatcgcga ccgggacaat cttgacgaag
      181 ccttcgtcgc gaatccagcg ttcctcccaa ccgtagtcga attcttcggc cgagccgcga
      241 aaatgatcga caaagtcaat cgtgagcgag tgtgagccaa ggaaattggc gatgctttcg
      301 ccgcggccga gcgcgagggc agcgcctgca tcgccgaagg acagctcctg ggaagaggca
      361 gcgcgcgcct tgcgcctctc gccagccacg atgagcggcc gggtggcacg acccgatctg
      421 agcgcatcga accccgcgac aagtgcggtc aagcccgcgc gctgtgacgc cgccacgtcg
      481 atcgcggata ttgcctcggg aagactcaag gctcgcgcga cgatggcagc gttgaggcgg
      541 tccgagtagg gctggcttgt agaggcgaaa tagagagcgt ccgtgccggc gcgatcgcgc
      601 agcggatctg ctgcgggcaa gcagtcgcgc ccggcttcca ccgccatggt cagcgcgtcc
      661 tcgtcccagc ctgccatcga cctctcgcct ttggcgcttg cgagaagtcc gggcgcgaac
      721 cacgcattgg cggcggccgc cgctttgcgc tgaagcctga gccgcggaat ataggcgccg
      781 tacgcggcaa tccccgtcat gaatccagcc tgtttcgatt tcgggcgaag ggcgagaaga
      841 ccagctttgc aggcggctgg caatgctgcc gtcctggctc atggacggcc cggcggccag
      901 tgtgcgatgt acgcaatccc cgtcgcgggc gggaaacact cgcaaacgag atgagagcca
      961 tgcccactcc gccgccagtc cgcaaggccg tattgcccgt cgcagggatg ggcactcgct
     1021 ttcttccggc aacgaaggca gtgccgaaag aaatgctgcc cgtggtcgac aagcccgtgg
     1081 tgcaatacgc tgtcgaagaa gccaaggaag ccggaatcga acaattcata tttgtcaccg
     1141 gccgcggcaa gtacatcatc gaggaccatt tcgatcatgc gcacgagctc gaaagcctgt
     1201 tgagctcgcg cgacaagaca gccgaactga acagcctgct ggaagacctg ccgatcacgg
     1261 gttccgtgag ctttacacgg cagcagaaac ctctcgggct cgggcacgca gtgtggtcgg
     1321 cgcgccattt tgtgggcaac gagccctttg cggtccttct gccggacgat ctgctggtcg
     1381 gtaagcccgg cgccatgacg cgaatgatcg aggcctacca cgcggtcggt ggtggaatga
     1441 tcgtggcggc cgaagacaag ccgctcgcgg aaaccggtcg atatggcgtg atcgagccag
     1501 gcgccactgt gggtgatgtg gtcgaggtga agggcgtggt ggaaaagccg gcacccgaaa
     1561 acgcgccatc ccggctgtgc atcatcggcc gatacatcct cggccccgaa atctttgccg
     1621 aactcgatcg gcaggaacgc ggcgcgggca acgaaatcca gctgactgat tccatggcgc
     1681 gcctgatcgg ccgcgtgcct ttccatgctg ttcgaaccgg ttgtgcgcgc tacgattgcg
     1741 gagacaaggt aggctttctg cacgccaaca ttgcggtcgg tctcacccgg cccgacattg
     1801 cacccgccct gcgcgcgatc ctgaaggacg tcgacttttg aaccgcggcc gaccgcgaaa
     1861 tatgcgcggg ccatcggcga tggccatgtg gcgcgacaaa catccctatc caagggcttg
     1921 ggagccaagg gccggttcat cggttacata ggacaagcga agggggaaac ccatgggtat
     1981 cccggcgaaa tccgtagcgc gtccgatgcg aatcggacat ttacttgcgg cgggcattgc
     2041 gtgcctgctc gccctcggcg ggccggcgct cgcgcgatct tcccggaccc agtcggggga
     2101 agccggcggc acaacttacg ccatccggtt cgatcattgg agcgaccaag acgaacgcga
     2161 tttcggcgaa ttcattgccg gcatcggcga ttcgggctgc acgaccgtcg acacctgtct
     2221 cagggatccg agcaatccat tccgggcatc cgatattgga gggtctttct tccaggcgga
     2281 ctgcgcgaag ctcatgtaca tcctgcgcgc ctatttcgcc tggaagcgcg gcctgccctt
     2341 ttcctacgcc aacggggtcg cgccggtggg gggcggagct gacgcgcgtt attcgcccga
     2401 aggcaaccgc gtcacctccc gcgcggaggt gttaacggga agcgcctccg gctatgcgct
     2461 cctgaacaac gtgctgagct cggtatcctc ggcaaactac cgcatgcatc ccgatctgga
     2521 ttccccgctc agaccggatt tctattcggt tgcgatcaat gccaagtccg tcaggccggg
     2581 caccgccatg tacgatccca acggccatgt gggcatcgtc tacaaggtgg aatccgatgg
     2641 acgtatccgc ctcatcggct cccatccgga caacacgctt acccgggact attacgattt
     2701 gcgcttcgtg cgcagccggc ccggtgtagg cgccggcttc aaaaactggc gcccggttcg
     2761 gcttgtcggc tacacgcgcc gaagcgatgg agcgctcgtg ggcgggcaca tggaactcgc
     2821 gtcaaattcc gagatcccgg atttctcgga cgaacaatat tacggcaacg gccagcggcc
     2881 aaccgacgag gactgggcga gcggtacgtt ctcgctgaac ggcgagacgg tcgactacta
     2941 cgactatgtg cgcgcgaaga tcgccggcgg acggcttgag ttcgacccgg tcaaggaagt
     3001 cgccgacatg gtggattcaa actgctcgga tcttcacgac agggtccaag cggtcaatat
     3061 cgcgcttcag gcaggcatcc
//
LOCUS       DASZMC010000100         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4994210,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000100 DASZMC010000000
VERSION     DASZMC010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4994210"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VG274_01855"
     CDS             <1..923
                     /locus_tag="VG274_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWE05421.1"
                     /translation="PVLHALERHVFSAERLHGDDTTVPVLAKGKTDIARCWVYVRDDK
                     PFGAKDPPAAMFYYSRDRKGEHPQAHLAGYAGPFQADAFDGYRQLYLRDRSPGPIIEA
                     ACWVHARRPFFAMADIAQNARRQAEGKQAVVLSPIAIEVVRRIDALFDIERAINGKSA
                     AERRAVRQELSKPLVEELQTYLQEQRAKLSRSHDLAKAIDYILKRWAAFTLFLDDGRV
                     CLSNNAAERALRGIALGRKSWMFCGSDRGGQRAAAMYSLIVTAKMNDIDPQAWLADVL
                     RRINEHPASKLDDLLPWNWRPSASSHSKAA"
     gene            complement(943..1419)
                     /locus_tag="VG274_01860"
     CDS             complement(943..1419)
                     /locus_tag="VG274_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05422.1"
                     /translation="MVLRSRRARSTHPAKAATLIAGTADRGTSAFPALGFWFIDGGGP
                     ELPEGTSDAEHTDVASISIDAISPKGEAFTIELEIGTPYEREPGGWACPVALTGLYDR
                     LAEARGVDSFQALCMAISLAQDLLHGFRDKGGKLLMDAENFPLEAYGFGAATRRGP"
     gene            complement(1410..1835)
                     /locus_tag="VG274_01865"
     CDS             complement(1410..1835)
                     /locus_tag="VG274_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05423.1"
                     /translation="MEIGDRWRRHAECRAAGVAIATFVAPVAFAASAVLAASAALAAS
                     ATSAALVAFRVLGFLQLRERVSLLAPLLLELRDLSFQGVRLVRLGLQRAIGVLFGDAI
                     LLGGRLLVRRLLGLSLHFSLASTFVLGATGMRSRQRSWC"
     gene            complement(2161..>2835)
                     /locus_tag="VG274_01870"
     CDS             complement(2161..>2835)
                     /locus_tag="VG274_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWE05424.1"
                     /translation="KLIAELVEVESGRKNDRPVIKEALWHSRVYGAKLVVARLDRLAR
                     STALIAGLMESGVDFVAADMPFANRFTLHIIAAVAEYEARLISERTKAAFAIAKAQGR
                     KFGSPRPGAGLSPAVLEAKVRKQRERAKAHALEFAPLLCELRDRGETINGIAAQLTLM
                     EIATPRDAKKWSHNLVRKMFERAGERKPKPRLGRRPKTKERPPKLTIQSLLRTLAPAT
                     RNFDCR"
BASE COUNT          550 a          903 c          887 g          495 t
ORIGIN      
        1 atccagtcct tcacgctctc gagcggcatg tattttcggc agaacgcctt cacggcgacg
       61 acacgaccgt cccggtgctc gccaagggca agaccgatat cgcccgatgt tgggtctatg
      121 tgcgcgacga caagccgttc ggcgccaagg acccgcctgc ggccatgttc tattactcgc
      181 gcgaccgcaa gggtgagcat ccgcaggccc atctggccgg atatgcaggg ccgttccagg
      241 ccgacgcctt cgacgggtat cgccagctct atctgcgtga ccgaagtccc ggcccgatca
      301 tcgaggcggc atgctgggtc cacgcccggc gcccgttctt tgccatggcc gatattgcgc
      361 agaacgctcg gcggcaggcc gagggcaagc aggcagtcgt gctctcgccg atcgccattg
      421 aggtcgtgcg ccgcatcgac gcgctcttcg acatcgagcg ggcgatcaac ggcaagagcg
      481 cggccgaacg cagggcggtt cgccaggagc tgagcaagcc gctggtcgag gagcttcaga
      541 cctatctgca ggagcagcgc gccaagctct cccgcagcca tgacctggcc aaggccatcg
      601 attacattct gaagcgctgg gcggccttca cgctcttcct agatgacggc cgcgtgtgcc
      661 tgtcgaacaa tgccgctgaa cgcgcgttgc gcggtattgc tctcgggcgc aagtcctgga
      721 tgttctgcgg atccgaccgt ggtggtcagc gcgccgccgc catgtacagc ctgatcgtca
      781 cggccaagat gaacgacatc gacccacagg catggctggc cgatgtgctc cggcggatca
      841 acgaacatcc ggcctccaag ctcgatgact tgctgccgtg gaactggcgt ccatcggcgt
      901 catcccatag caaagcggcg tgatagttag cggcgaccag gcttatggtc cccgtcgggt
      961 tgccgccccg aaaccgtatg cctcaagcgg aaagttctca gcatccatca ggagcttgcc
     1021 gcccttgtca cgaaaaccgt gcagcaagtc ttgtgcgaga ctgatcgcca tacacagagc
     1081 ttgaaatgaa tctacaccac gcgcttccgc gaggcggtcg tataggccgg tcagagcaac
     1141 cgggcaggcc cacccaccgg gttcacgttc gtaaggtgtt ccgatctcaa gctcgatggt
     1201 gaacgcctcg ccctttggcg agatcgcgtc gatcgaaatg ctcgctacgt cggtgtgctc
     1261 agcatctgac gttccttcgg gcagctctgg cccgcctccg tcaataaacc agaagccgag
     1321 ggcggggaat gcagatgtgc cgcgatcggc agtgccagcg atcagggttg cggccttcgc
     1381 cggatgcgta ctccgcgcgc ggcgcgacct cagcaccacg aacgctgccg cgaacgcatg
     1441 cccgtcgcgc ccaatacgaa cgtcgacgcg agcgaaaagt gtagggaaag gccaagcagg
     1501 cggcggacga gcaggcgacc gccgagcaaa atcgcgtcgc caaacaggac gccgatcgcg
     1561 cgttgcaagc cgaggcggac aaggcgcacg ccttgaaagc tcagatcgcg cagctcgagc
     1621 agcagcggcg caagaaggct gacgcgctcg cgcaattgca aaaagccgag gacgcgaaaa
     1681 gccacgagcg cggcggaagt cgcggaagcg gccaaggccg cagaggctgc taaaaccgcc
     1741 gaagctgcaa aggccaccgg agctacgaaa gtcgcaatgg cgaccccagc cgcgcggcac
     1801 tcagcgtgac gccgccagcg gtcaccgatc tccatgcggt gctcgtgtcg atcgaaggcg
     1861 aactgcgtcg gctcggatgc tgcggcggcg gtgacgcaga ttggaattct gcgccggacg
     1921 acggtctact cgcgaatctg gaaagccagc agggccaagt ttgcccctcg aatgttccgc
     1981 gcgcgaagtc gcgcacgaag gacgatgcgt cgccaagacc taccggacgg gcgaaattct
     2041 gtcccgcgac ggctctgcct gcgtaggtcg ggcgcctcgg ccgcaggccg cagccatggt
     2101 ccgcgccgcc gcgcccaggt tacccgcagc cccgcggcgc aaaagggcca ctgcttagct
     2161 tcaacggcag tcaaaattgc gagtagctgg ggctagtgtc ctcaacagtg attgaatcgt
     2221 aagcttcggc ggccgttcct tcgtcttcgg ccggcggccg aggcgtggct tcggcttccg
     2281 ctcgcccgct ctttcgaaca tcttgcggac gagattgtgg ctccattttt ttgcgtcgcg
     2341 gggcgttgcg atctccataa gcgtgagttg agcggcaatc ccgttgatgg tctcgcctct
     2401 gtcgcgaagc tcgcagagaa ggggagcgaa ctcaagagcg tgagccttgg ctcgttcgcg
     2461 ttgttttcga actttggcct ccaacactgc aggcgaaagg cccgcgccgg gccttgggct
     2521 gccgaatttt cttccttggg ctttcgcgat ggcgaacgca gctttggtgc gttccgaaat
     2581 caagcgagcc tcatattcgg cgaccgccgc gatgatgtgg agcgtgaaac gattcgcaaa
     2641 cggcatgtcg gccgccacga aatcaactcc gctctccatg aggcccgcaa tcagagctgt
     2701 actgcgcgcc agtcgatcca ggcgagcaac aacaagcttc gcaccgtaaa cccgggagtg
     2761 ccaaagagct tccttgatga cggggcggtc gttcttacga ccactttcga cttcgacgag
     2821 ttccgcgatc agctt
//
LOCUS       DASZMC010000101         4816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_604616,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000101 DASZMC010000000
VERSION     DASZMC010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604616"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VG274_01875"
     CDS             <1..796
                     /locus_tag="VG274_01875"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HWE05425.1"
                     /translation="RLLDAMRYAALGGGKRMRGFLVQQSGRLFGVDRRALGRVSAAVE
                     CVHAYSLVHDDLPAMDDDDLRRGKPTVHKAFDEATAILAGDALLTMAFGLLAAPDAHG
                     DPFVRCELVVRLAHASGHAGMVGGQMMDIAFEHASPGLPEITRLQRMKTAALITFSCE
                     AGAILGRASASARNALSAYGQDLGLAFQIVDDLLDAEGDPAKMGKAAGKDADRGKPTV
                     VSVLGADRARAQAEALAVQAAGHLDLFDERADLLRAAAEFVISRPA"
     gene            811..2748
                     /gene="dxs"
                     /locus_tag="VG274_01880"
     CDS             811..2748
                     /gene="dxs"
                     /locus_tag="VG274_01880"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWE05426.1"
                     /translation="MSLPATGLPTKTPLLDTIKEPRDLRKLSRAQLRQVADELRQETI
                     AAVAVTGGHLGAGLGVVELTVALHHVFDTPHDKLIWDVGHQAYPHKIITGRRDRIRTL
                     RQGGGLSGFTKRSESIYDCFGAAHSSTSISAGLGFAVARDQKKETHNVVCVIGDGAMS
                     AGMAYEAMNNAGAINARLIVVLNDNDMSIAPPVGAMSAYLAKLISSRPYRGIRRVAKQ
                     LAQLLPRPMENTARRAEEYARGIAMGGTLFEELGFYYVGPIDGHNLDHLLPVLENVRD
                     AQNGPILVHVVTQKGKGYPPAEASADKYHGVVKFNVLTGEQSKSPAKAPSYTKVFAEA
                     LIAEAEKDERIIAITAAMPSGTGLDLFAKRFPDRCYDVGIAEQHGVTFAAGLAAEGLK
                     PFATIYSTFLQRAYDQVVHDVAIQSLPVRFAMDRAGLVGADGPTHAGSFDVTYLATLP
                     GFVVMAAADEVELTHMVATAAQIDDRPSAVRYPRGEGLGLARPAAGTPLPLGVGRVMR
                     EGTTIALLSLGTRLAECLAAAERLSGYGLSATVADARFAKPLDKDLIRRLAREHEVLI
                     TVEEGSVGGFAAHVMQFLAWEGLLDAGLKLRPMILPDRFIDHDSPERMYESAGLDSRA
                     IVATALGALGRPDVAVAGERA"
     gene            complement(2984..3625)
                     /gene="plsY"
                     /locus_tag="VG274_01885"
     CDS             complement(2984..3625)
                     /gene="plsY"
                     /locus_tag="VG274_01885"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HWE05427.1"
                     /translation="MFDALNTIDWQDRAPTFFAALVLGYLVGSIPFGLLFSWASGAGD
                     VRKMGSGNIGATNVLRTGKRWAALATLVCDGGKGLLAVLFVGTLHGDVAAVFAGLGAF
                     LGHVFPMWLRFRGGKGVATFLGVTLALAWPVGLLALATWLAAAAAWRISSLAALVTAA
                     LVPVYMMLFSEPLYAIFELLLAFFIFALHRGNIGRIASGREPRIGTKVEMRSV"
     gene            complement(3923..>4816)
                     /locus_tag="VG274_01890"
     CDS             complement(3923..>4816)
                     /locus_tag="VG274_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE05428.1"
                     /translation="AGEQMTEIGLLTEAGAVAFTDGDRTITNSRVMRLALAYASTFGA
                     LVVGHAEDPELSEDAAMNEGEFATRLGLPAAPPAAETIIVERDIRLVELTGARYHFGQ
                     ISTRAALEAIVAAKSRGLPITCGASACHLALNELDVGSYLTFRKVKPPLRSEADREAM
                     VEGVAAGEIDVIVSSHDPQAADTKRQTFAHAAFGAIGLETLLPVTLQLFHNGKAGLLP
                     LLATLTSTPARILGLESGRLACGLPADLVLFDPEAPFAVDAAALHSRARNTPFEGQTF
                     QGRVHQTYVDGIRVFDRAAKR"
BASE COUNT          918 a         1541 c         1535 g          822 t
ORIGIN      
        1 ccggcttctc gacgccatgc gctacgccgc gctggggggc ggaaagcgta tgcgcggatt
       61 tctggttcag cagagcggcc ggctgttcgg tgtcgatcgg cgcgccctcg gccgggtatc
      121 ggccgccgtc gaatgcgtcc acgcctattc gctcgtgcat gacgatctgc ccgcgatgga
      181 cgacgatgat ctcagacgcg gaaaaccaac ggttcacaag gctttcgacg aagcaaccgc
      241 gatacttgcg ggagacgccc tgctgacgat ggcgttcgga ctactggccg cgcccgacgc
      301 gcatggcgat cccttcgtgc ggtgcgaatt ggtagtccgg cttgcccacg cctcaggtca
      361 tgccggcatg gtcggcggac agatgatgga catcgccttc gagcatgcct cccccggtct
      421 gccggagatc acccggctgc agagaatgaa gaccgcggcg ctgattacct tcagttgcga
      481 ggccggggcc attctgggcc gtgcatccgc ctccgcccgc aatgcgttat cagcctacgg
      541 tcaggacctc ggcctggcgt tccagatcgt ggacgatctg ctggatgccg agggcgaccc
      601 ggccaaaatg gggaaagccg cgggaaagga tgcggatcgc ggcaagccaa ctgtcgtctc
      661 tgttctgggg gccgacagag cgcgtgcgca agccgaggcg ctggcggtcc aggccgccgg
      721 tcaccttgat ttgttcgatg aaagggccga ccttctgcga gcagcggctg agttcgtgat
      781 ctcgcggccg gcataatcgg agcgaaacga atgagcttgc cggcaacagg cttgcccaca
      841 aagacgccgc ttctcgacac gatcaaggag cctcgggatc tccgcaaact gtcgcgcgcc
      901 cagctgcggc aggtggccga cgaactgcgc caggaaacca ttgccgccgt cgcggtgacc
      961 gggggtcatc tgggagcggg actgggcgtg gtcgaactca cggtcgcgct gcaccatgtt
     1021 ttcgatacgc cacacgacaa gctgatctgg gacgtgggcc accaggccta tccccacaag
     1081 atcatcaccg gacgccgcga ccgcatccgc acattgcggc agggcggcgg cctgtccggt
     1141 ttcaccaagc gcagcgagag catctatgac tgctttggcg ccgcgcactc ctccacttcg
     1201 atctccgccg gcctgggttt cgcggtggcg cgcgaccaga agaaggagac gcacaacgtc
     1261 gtttgcgtaa tcggcgacgg cgcaatgagc gcaggcatgg cgtatgaggc gatgaacaac
     1321 gccggcgcca tcaatgcccg cctcatcgtt gtgctcaacg acaacgacat gtcgatcgcg
     1381 ccgccggtcg gcgcgatgag cgcctatctt gccaagctga tttcgagccg cccctatcgc
     1441 ggaatccggc gcgtggcgaa gcagctcgcc cagctgttgc cccgtccgat ggaaaacacc
     1501 gcgcgccgcg cggaagaata tgcccgcggc atcgcgatgg gcggcaccct gttcgaagag
     1561 ctgggattct attacgtcgg gcccatcgac gggcacaatc tggatcatct gctgcccgtg
     1621 ctcgagaacg tgcgcgacgc gcagaacggc cccattctcg ttcacgtggt gacgcaaaag
     1681 ggcaagggct atcctcctgc cgaagcatcg gcggacaaat atcatggcgt cgtcaagttc
     1741 aacgtgctca ctggcgagca gagcaagtcg ccggcaaagg cgcccagcta caccaaggtt
     1801 ttcgccgaag ccctgatcgc cgaggcggag aaggacgaac gcatcatcgc gatcacggcg
     1861 gccatgccgt ccggaaccgg gctcgatctg ttcgcaaagc ggtttcctga ccgttgctac
     1921 gacgtgggca tcgccgaaca gcacggcgtc acatttgcgg caggactcgc ggcagagggt
     1981 ctgaagcctt ttgccacgat ctactcgaca ttcctgcagc gggcctacga tcaggtcgtg
     2041 catgacgtgg cgatccagtc gctgccggtg cgattcgcga tggaccgcgc cggcctcgtc
     2101 ggcgcagacg gtcccaccca tgcgggctcc ttcgatgtga cctacctcgc gacgttgccg
     2161 gggtttgtcg tcatggccgc agcagacgaa gtggaactga cgcatatggt tgccactgcc
     2221 gcacagatcg acgaccggcc ttcagccgtc cgttatccgc gcggagaggg attgggtctg
     2281 gcgcggcctg ccgctggaac acctctgccg ctcggcgtgg gacgtgtcat gcgcgagggc
     2341 acgacgatcg cgctgttgag cctcggcacg aggcttgccg aatgcctcgc cgccgccgag
     2401 aggctgtcgg gctacggatt gtccgcgacc gtcgcggatg cgcgatttgc caagccgctc
     2461 gacaaggatt tgattcgcag actcgcgcgc gaacacgaag ttctcatcac cgtggaagaa
     2521 ggctccgtgg gcggcttcgc ggcgcacgtc atgcaattcc tcgcctggga aggattgctc
     2581 gatgcggggc tgaagctcag gccgatgatt ctgccggacc gcttcatcga tcacgattcg
     2641 ccggagcgga tgtacgaatc cgccggtctc gattccaggg ccatcgtcgc caccgcactc
     2701 ggcgcactcg gcaggccgga tgtcgcggtg gcaggcgagc gcgcgtgagg gcacgaaagc
     2761 ccctcaccca cgcttgaaca gaattcatcg cccgtatctg catccctctc ccatccgcct
     2821 acgccaaggc ttcggcggac caagtgagcc gccagacccg ccgtaccccc ggatcacagt
     2881 ccggggggaa ggcgagctgc gcatcctctg cgtcctccgc gttgaatctt cccaccttca
     2941 tgatctttgt ggtcttggtg gtgaatcatt gggacgatct gactcaaacg gagcgcattt
     3001 cgactttggt tccgatgcgc ggctccctcc cgctggcgat gcgcccgatg ttgccgcggt
     3061 gcagggcgaa gatgaagaag gcgaggagaa gttcgaagat tgcatagaga ggttcgctga
     3121 agagcatcat atagacggga accagcgcgg ccgtgacgag agcggcgagg gaggagatgc
     3181 gccaggccgc cgcggccgcc agccacgttg caagcgcgag cagaccgact ggccaggcga
     3241 gcgcgagcgt gacgccgaga aaggtcgcga cgcccttgcc gccccgaaag cgcagccaca
     3301 tcggaaacac atggcccagg aaagctccca gtccggcaaa caccgccgcc acgtcgccat
     3361 gcagtgtgcc gacaaaaagg acagccaaca gtcctttccc gccatcgcag acaagcgtgg
     3421 cgagcgccgc ccagcgcttg cccgtgcgca ggacgttggt cgcgccgata ttgcccgaac
     3481 ccatcttgcg cacgtcgccc gcgccggatg cccaggagaa caacagaccg aacgggatcg
     3541 atccgacgag atagccgagc accagagcag caaaaaaggt cggcgcccga tcctgccaat
     3601 cgatcgtatt gagagcgtcg aacatcagcc gggctccgtg ggcggcctat agtctaatac
     3661 ccgaaatcgg agttgggtga tacgcataaa agattcaacg cagggaacgc agaggttgcg
     3721 ccgagggcgc agagaaaaac tggcgcgctt cctacgctgc ttcgcagctt cggaggacat
     3781 cctcgctcgc ccgccgaagc tttagcgcag gcgggttgcg cgcccatatt ttcaccgcgt
     3841 cctctgcgca acctctgcgt cccctgcgcc aagaatcttg tcggttgacg aatcgcatta
     3901 cttcagaatt gggtgctagc cgctatcgtt tcgccgcgcg gtcgaaaacg cgaattccgt
     3961 cgacatatgt ctggtggacg cggccctgga aggtctgccc ctcgaatggc gtgttacgcg
     4021 cgcgcgaatg cagagctgcg gcgtcgactg caaacggcgc ttcggggtcg aacagcacga
     4081 gatcggccgg caaaccgcac gcaagcctgc cgctttccag ccccagaatg cgcgccggcg
     4141 ttgaagtcag cgttgccagc agaggcaaca gacccgcttt cccgttgtga aacagctgaa
     4201 gggtaaccgg aagcagcgtc tccagcccga tggcgccgaa agccgcatgc gcaaaggtct
     4261 gccgtttggt gtccgccgcc tgcggatcat gacttgacac gatcacgtcg atttcgccgg
     4321 cagcgacccc ttcgaccatt gcttcgcggt ccgcttccga acgcagcggc ggcttgacct
     4381 tgcggaaagt aagatacgaa ccgacatcga gctcgttgag cgcgagatgg caggcggagg
     4441 cgccacaagt gattggcagt ccccgcgact tggcagcgac aattgcctca agcgcggctc
     4501 gcgtcgaaat ctgcccgaaa tggtagcgcg cgccggtcag ctccacgagc cgaatatccc
     4561 gctcgacaat gatcgtctcc gctgctgggg gtgcggcggg aaggccgagc cgcgttgcga
     4621 actcgccttc gttcatcgca gcgtcttccg aaagctcggg atcctccgcg tgaccgacga
     4681 cgagcgctcc gaaggtcgag gcataggcga gcgcgaggcg catcacgcgc gaattggtga
     4741 tggtgcggtc gccatcggtg aaagcaacgg cgcccgcttc cgtcagcagc ccgatttcgg
     4801 tcatctgctc gccggc
//
LOCUS       DASZMC010000102         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3070669,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000102 DASZMC010000000
VERSION     DASZMC010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3070669"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..596)
                     /locus_tag="VG274_01895"
     CDS             complement(111..596)
                     /locus_tag="VG274_01895"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HWE05429.1"
                     /translation="MDFNKLWANFMDTVQNHYMDFNGRIPRAQFWYYILVVVVIGVAA
                     GVIGGIAGTTLLSAVVSLGLLLPNAGMAARRMQDTGRSGQLVWVWVIVGGLYGVMALL
                     LGLAGPFGALAFLAFFFTIGWLINLAMLIVSIAIIYFCAQPGQPDANAYGPVPPVWTP
                     N"
     gene            744..1754
                     /gene="argC"
                     /locus_tag="VG274_01900"
     CDS             744..1754
                     /gene="argC"
                     /locus_tag="VG274_01900"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWE05430.1"
                     /translation="MVAKVFIDGGVGTTGLEIRERLAGRSEFSLLHLSDTDRKDTKAR
                     AGALNDADVVILCLPDEAARDAVALIDNPSVRVIDASSAHRVAEGWVYGFPELERDQR
                     ARIAAATRVSNPGCWPTGFLALVRPLVRAGIVPADFPLTVTGVSGYSGGGKAMIEEFE
                     ANNSPRYVDTVVRTYALTLSHKHQPEMRVHAGLAHPPVFSPNVGRFYRGMIVEVPLQL
                     WALPAKPNVFDLYREIADAYRGERLIEVAGRDKFDPLMTLKKAAQAGQMPPPVSALSQ
                     SIDAESLKLTNGMKLYVLGNDKTQQARLIAVLDNLGKGASGAAVQNLNIMCGLPETAG
                     LV"
     gene            1754..2377
                     /locus_tag="VG274_01905"
     CDS             1754..2377
                     /locus_tag="VG274_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HWE05431.1"
                     /translation="MYRPSAFATDEVTALHDVIRQRAFATVACVIDGTIALAYAPVVL
                     DVDTGPRGSVRFHLAGINPVAAVPDGTPFTLSFLGPDAYVSPDWYETHGRVPTWNYLA
                     VEGRGVVQRMSGDQLRQLLINLSAVEENKLLPKKPWTIDKVPEDKMDALMNAIVGFSV
                     RFETLEGKFKLSQNVTPEDAEGVMRGLIQRGNANGRGIAKAMRRTRT"
     gene            2374..>3000
                     /locus_tag="VG274_01910"
     CDS             2374..>3000
                     /locus_tag="VG274_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05432.1"
                     /translation="MKKWLSVCAVLIASPAQAADYLPPLGDAWAAHTAAQEGFDAAKL
                     QAAIDFAVMHETKLSPSLDGVIDQRDLRITVPLQFAGPFSDPIGPLTTHAPANGIIIR
                     HGYIVAEWGDTRKVDMAHSVTKTFLSATAGIAFDKHMIRDVNDRVLDYVHPSPDFELP
                     HNQPITWDEMLRQTSGWVGTMWGKPWWADRPGKNPWDELAAGPPPVGKE"
BASE COUNT          615 a          868 c          954 g          563 t
ORIGIN      
        1 gcggtctatc ggctgcgcga agccgcctga ttttctttag cgatccacaa aacaaaaagc
       61 ggcccacgcc gtttggcgcg agccgctttc ttataaggct ctgacgacgg ttagtttggt
      121 gtccacaccg gcggaacggg gccataggcg ttagcgtcag gctgaccggg ctgcgcgcag
      181 aaatagatga tcgcgatcga gacaataagc atcgcgaggt tgatcagcca gccaatggtg
      241 aaaaagaacg ccaggaaggc gagcgcgcca aaggggccag ccaacccaag cagcagggcc
      301 atgacgccat aaaggccgcc gacaatcacc cacacccaga cgagttggcc acttctgcct
      361 gtgtcctgca tgcggcgcgc tgccattccg gcgttcggca gaagaaggcc aaggctgacg
      421 acggcgctca gcagggtcgt accggcgata cccccgataa cgcctgccgc aacaccgatg
      481 acgaccacga caagtatgta ataccagaac tgggcccgag gaatgcgtcc attgaagtcc
      541 atgtaatggt tctggacggt atccatgaaa ttcgcccaaa gcttattgaa atccatttgc
      601 ttctccccgc gaggcgatag cctcgtccct ggttgcggcg gcgcgattct aaagactcgg
      661 aatcggcact cgggttgagc taaaaggccg cgtaatgttc tgtcaatgac gcgcacgcga
      721 gccgcccggg aacaaacaga tttatggtag cgaaagtctt tatcgacggg ggcgttggca
      781 cgacgggcct cgaaatccgc gagcggttgg caggccgtag cgagttttcg ctcctgcatc
      841 tgagcgatac cgaccgcaag gatacgaaag cccgcgcggg cgcgttgaac gacgcggatg
      901 tcgtcatcct gtgcctgccg gacgaggcgg cgcgcgatgc cgtggcattg atcgacaacc
      961 cttccgtgcg cgtgatcgac gcatcgagcg cgcaccgtgt cgccgagggc tgggtctatg
     1021 gctttcccga attggaaagg gaccagcgcg cgcgtatcgc ggcagcgaca cgggttagca
     1081 atcctggttg ctggccgacc ggatttctgg cgctggtgcg gccgttggtg cgtgctggaa
     1141 ttgtgcctgc cgattttccg ctgaccgtaa cgggtgtctc cggctactcc ggcggcggca
     1201 aagcgatgat cgaagagttt gaagcgaaca attctccgcg ctatgtcgat accgtcgtgc
     1261 gcacctatgc gctgacgttg tcgcacaagc accagccgga aatgcgcgtg catgcggggt
     1321 tggcgcaccc gccggtgttc tcgccgaatg tcggacgttt ctatcgcggc atgatcgtgg
     1381 aagtgccgct tcaactttgg gcgttgccgg cgaagccgaa cgtgttcgat ctctataggg
     1441 agatcgcgga tgcctatcgc ggcgagaggc tgattgaagt cgcgggtcgc gataagttcg
     1501 atccgctgat gactctaaag aaggccgcgc aggcagggca gatgccgccg ccggtcagcg
     1561 cgttgtcaca aagcatcgac gcggagtctc tgaagctcac caacggcatg aagctctatg
     1621 tcctcggcaa cgacaagaca cagcaggcgc gcctgattgc ggtactcgac aatctcggca
     1681 agggcgcgag cggcgcggcg gtacagaacc tgaacatcat gtgcggcctg ccagagacgg
     1741 cgggcctcgt gtaatgtatc gcccttcggc atttgcgacg gacgaagtga cagcgctgca
     1801 cgatgtgatc cgccagcgcg cgttcgcgac agtggcctgc gtgatcgatg gcacaatcgc
     1861 cttagcctac gcgccggttg tgcttgatgt ggacactgga ccgcgcggca gcgtgcgttt
     1921 ccatttggct ggcatcaatc cggtcgcggc ggtgccggat ggtacgccat tcacgctctc
     1981 tttcctcggt ccggatgctt acgtctcgcc agattggtac gagacgcacg gccgtgtccc
     2041 gacgtggaat tacctcgccg tcgagggtag aggtgtggtg cagcgcatga gtggagatca
     2101 gttgcgccag ttgctcatca atctatcggc cgtggaagaa aataagctct tgccgaagaa
     2161 gccgtggacc atcgacaaag tgccggaaga caagatggac gcgctgatga atgccatcgt
     2221 cggtttctcg gtgcgattcg aaacgctcga aggcaagttc aagctctcgc agaacgtcac
     2281 acccgaagac gccgaaggtg tgatgcgtgg tcttattcag cgcggcaacg caaacggccg
     2341 tggcatcgct aaggccatgc gaaggacgcg gacatgaaga aatggctttc tgtctgcgcg
     2401 gtacttatcg cgtcgccggc gcaggccgcg gactatctcc cgccgcttgg cgacgcatgg
     2461 gcggcgcaca cagccgcgca ggaaggcttc gatgctgcga agcttcaagc cgcaatcgac
     2521 tttgccgtca tgcacgagac caaactgtcg ccatcgctcg atggcgtgat cgaccagcgc
     2581 gatctgcgca tcacggtgcc gttgcaattc gccggaccgt tctccgatcc catcgggccg
     2641 ttgacgaccc atgcgccggc gaacggcatc atcatccgtc acggctacat cgtcgcggag
     2701 tggggcgata cgcgaaaagt cgatatggcg cattctgtga cgaaaacgtt cctgtcggcg
     2761 acggcgggca ttgcgttcga caagcacatg atccgcgatg tgaacgatcg tgtgctggac
     2821 tatgtgcatc cgtcgccgga tttcgaacta ccgcacaatc agccgatcac atgggatgag
     2881 atgctgcgtc agacgtccgg gtgggtcggc acgatgtggg gcaagccgtg gtgggccgac
     2941 cggccgggca agaatccgtg ggacgaactc gccgcaggcc cgccgccggt cggcaaggaa
//
LOCUS       DASZMC010000103        10443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1697494,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000103 DASZMC010000000
VERSION     DASZMC010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697494"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..2090
                     /gene="nuoL"
                     /locus_tag="VG274_01915"
     CDS             81..2090
                     /gene="nuoL"
                     /locus_tag="VG274_01915"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWE05433.1"
                     /translation="MFLPLVGAAVAGLFGRLIGARTSEIVTTGLLFVSAALSCWAFYD
                     VIFMGHPTLVRVMEWIVSGSFEADWAIRVDALTAVMLVVVTGVSALVHLYSVGYMHDD
                     AHRPRFFAYLSFFTFAMLALVTSNNFLQLFFGWEGVGLSSYLLIGFWYTRPAANAAAI
                     KAFVVNRVGDFGFALGIFGVYAIFHTLDFSSVFAAAPAMAGKTFLFAGHQLDILTTLC
                     LLLFMGAMGKSAQIGLHTWLPDAMEGPTPVSALIHAATMVTAGVFLVCRCSPMFEHAP
                     IALAFVTFIGATTAFVTASIGLFQNDIKRVIAYSTCSQLGYMFAAAGVSAYGAAMFHL
                     FTHAFFKALLFLGAGSVIHAMHHEQDMRRMGGLAPMLPFTWAMMLVGNLALTGFGLPF
                     TPIGTAGFYSKDAIINAAFLHGTPMATYAFWLTLTAALMTSFYSWRQFLMTFHGSWRG
                     ALEEKYASAEDDPHMYHEPLAGADRGHALRLSEVRESPLTMLVPLLVLAIGALIAGAL
                     FAHDFIGADTAGFWHGTIAAVTGHAGAAATLPLWVEVAPFVLTILGFAIAYYYYVLHP
                     ELPPQMAQRGGRVYTFLYNKWYFDELYDAVFVRPALWIGRLLWKGGDERVIDGIGPDG
                     VAARVIDATREAVRLQSGYVYHYAFAMLVGVVVLATWFMFTVGAR"
     gene            2087..3844
                     /locus_tag="VG274_01920"
     CDS             2087..3844
                     /locus_tag="VG274_01920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWE05434.1"
                     /translation="MSHLPLLSVVNFLPLAGALVILVTRGSRETQARSARATALAATV
                     VNLAVALFIWSRFDGSTAAFQFVEQAVWLGNGISYRVGVDGISLLFVVLTAGLMPFCI
                     LASWKSVTERVPEYMIAFLLLETMMIGVFTSLNLVLFYVFFEGGLVPMFLIIGIWGGN
                     RRVYASFKFFLYTLLGSLLMLLAIISMYLETGTSDIDVLIRTAHFSVAMQTWLWLAFF
                     ASFAVKMPMWPVHTWLPDAHVEAPTAGSVILAGILLKMGGYGFLRFSLPMLPNASEAF
                     APLVFVLSVVAVIYTSLVALAQEDMKKLIAYSSVAHMGLVTMGIFSLTQQGIDGGIFQ
                     MLSHGIISGALFLCVGVVYDRMHTREIGAYGGLVERMPVYAVCFMVFTLANMGLPGTS
                     GFIGEFLTMLGTFQVNTWVVIFAATGTILSAAYMLWLYGRIIFGALVKPALTAIQDLS
                     AREIAIFTPLVVLTIAFGVYPKPIFDVTSASVANLVAQHKLAMMNRGKPADHLHLAFL
                     NMPAKDPRGDTETRRKGSQGASEAVRQESRAFGAQSFSLSHRLIVNSSRSAPSAPPRA
                     HPWEPPIGADSSSSYEDVR"
     gene            3841..5283
                     /gene="nuoN"
                     /locus_tag="VG274_01925"
     CDS             3841..5283
                     /gene="nuoN"
                     /locus_tag="VG274_01925"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HWE05435.1"
                     /translation="MSGISSDLLVLLPECLLALAAMALLLIGVLSTKHAFRIVSTAAI
                     FALAVAAWFVVSPVSASPSAFHGAFVADGFARFAKLLVLLGSALTILMSQEFLEREKI
                     ANFEFPVLVLLATIGMLFMVSAASFIALYLGFELQSLSLYVLAAFNRDSLRSSEAGLK
                     YFVLGALSSGMMLYGISFIYGFAGTTAFPEVARAVTHAGANIGMIFGLVFLIAGLAFK
                     VSAVPFHMWTPDVYEGSPTPITAFFSIAPKTAAMALFLRVMIEAMPGAIHEWQQIIVF
                     VSVLSMFLGSVAAIGQGNIKRLMAYSSIAHMGYALIGIAAGTFQGVEGVLLYLLIYVI
                     TNAGVFVCILAMRRNGQMVENIPDLAGLARTNPRLALAFAVLFVSLAGIPPFAGFWAK
                     FYVFLAAIGAHLYALAILGVLASAIGAYYYLRIVKIMYFDEPAPAFDREMGLRLGGIA
                     VGASLFNLVFIVIASPLVAAAANAAKTLIP"
     gene            5280..6029
                     /locus_tag="VG274_01930"
     CDS             5280..6029
                     /locus_tag="VG274_01930"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWE05436.1"
                     /translation="MTEISASLRGIPLRHFKEIDSTNEEARRLALGGVEGPLWITADR
                     QTKGRGRRGRDWQSPEGNLAATLLLRPATPIAQCAQLSFVAALGVSEFVGRCAPRAEV
                     KVKWPNDVLADEKKIAGILLESASKDGARPDWLAIGFGINLALHPADTEFPATSIAGL
                     GEKPPAPRVALDCLAASWSRWYEMWFERGFEPIRDAWLARAARLGARIRARIADMEAV
                     GVFESIDADGALILRESNGRARLISAGEVFF"
     gene            6221..7015
                     /locus_tag="VG274_01935"
     CDS             6221..7015
                     /locus_tag="VG274_01935"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWE05437.1"
                     /translation="MLLAIDAGNTNIVFAICEGREIRAEWRAVMDQARTADEYAVLLN
                     PLLSLAGLKFADLDCAIIATVVPAALFDLRLFCKRYLHCEPLVVGEPDVDLGIEIRVD
                     RPNAVGADRLVNTVAAHERYKGALIVVDFGTATTFDVVSAVGNYEGGVIAPGVNVSAE
                     ALHLGAAMLPRVAIQRSQSVIGKDTVPAMQSGLYWGYLGLVEGLVARIRAEYGEPMTV
                     IGTGGLAGLFYRQTGAIEHLDEDLTIRGLVLIHARNTNAGPARLHR"
     gene            7015..8673
                     /locus_tag="VG274_01940"
     CDS             7015..8673
                     /locus_tag="VG274_01940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWE05438.1"
                     /translation="MDARLNESELVLLPLGGAGEIGMNFNAYGCGAPDDRRWIVVDCG
                     VMFGRESATPGVDLILPDIRFLAEQRENVLAIVLTHAHEDHIGAIAQLWPLLRCPLYA
                     TPFTARLIENKLAEAGLAERVRVTVVPLGGRIAIGPFEIDFISITHSIPEPNALAIRT
                     PFGVVVHTGDWKLDPDPLIGEVTDSESLRALGDDGALALVCDSTNALVAGESGSEARV
                     RTSLVDLIGTLTGRVAVTAFASNVARLESVGKAARTHGRSLALVGRSMNRVVEAARET
                     GYLHDFPPLIDADEAAQLPPNRVLYLCTGSQGETRAALSRVAAGEHPTVALGPGDTVI
                     FSSRIIPGNDLAIFEVQNKLSALGVEVLTERDHFVHVSGHPCRDELAQMYRWVRPKIA
                     VPVHGELRHMTEHARLARSLQVPQAIVLHNGHMLRLAPGRAEIVDETPSGRMHLDGKV
                     LVEEGKGHARARGALGYAGLVAATIALDHKGRVAGEPSLILEGIPDQVHGAIRTALAD
                     VIKRHESKRRDEQALKEQARLAIRRAAMAHWGKKPVTRVEVVWV"
     gene            8684..9088
                     /gene="mce"
                     /locus_tag="VG274_01945"
     CDS             8684..9088
                     /gene="mce"
                     /locus_tag="VG274_01945"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWE05439.1"
                     /translation="MIGRLNHVAIATKDIAAAAKVYRDTLGAKVSEPVAQPDHGVTTI
                     FVELPNSKIELIEPLGAASPIANFLRKNPAGGIHHICYEVDDIIAARDRMRAAGATIT
                     GTGEPRIGAHGKPVIFLHPKDFVGTLIELEQA"
     gene            9085..9306
                     /locus_tag="VG274_01950"
     CDS             9085..9306
                     /locus_tag="VG274_01950"
                     /inference="COORDINATES: protein motif:HMM:NF018978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HWE05440.1"
                     /translation="MRLVVLAGAFVILWFLALHILLPIGISPSHDGVGDPGAPANPRM
                     ALKLALATVAAAAMWAIFYVLVLLRVINI"
     gene            complement(9490..>10443)
                     /locus_tag="VG274_01955"
     CDS             complement(9490..>10443)
                     /locus_tag="VG274_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWE05441.1"
                     /translation="HIELYTHDVLFNPLPTFHCFGLTVGALMPLLLGIKAVFHPTPLQ
                     PREIARRIRQSGATILLSTDTFISQTARAGDQGDLNSLRLAVCGAERLRDETRTLVRK
                     KYSIELLEGYGVTEAAPVIAANQRGMNRPGTVGRLLQGIESRLEPVEGIPHAGRLVVR
                     GPNVMLGYIRPEQPGTFAPPPGGWYDTGDVVSIDPDGFIAIKGRLKRFAKIGGEMVSL
                     AVVESCASAMWPDYSHAALSVPGGRKGEEVLLVTTCPDAERTQFAGWVHNHGVQELAI
                     PRRVVLVESIPVIGTGKTDYAKLTKTLEETGALAEQETARA"
BASE COUNT         1636 a         3368 c         3273 g         2166 t
ORIGIN      
        1 tggtctattt ccgcaatcgc ggcacgatcg ccgtcgagac cgtcaatctg atgaagggct
       61 gaggcgtgta ccacgcgatc gtgttccttc cgcttgtggg cgcggctgtc gcgggcctgt
      121 tcggccgcct catcggcgcg cgcacgtcgg agatcgtgac caccggcctg ctgttcgttt
      181 cggcggcact ctcctgctgg gccttctacg acgtgatttt catgggtcat ccgacgctcg
      241 tgcgcgtcat ggaatggatc gtgtcgggaa gtttcgaggc cgactgggca atccgcgtgg
      301 acgccctgac tgccgtaatg ctggtggtgg ttacgggcgt ctccgcgctc gtccatcttt
      361 attcggtcgg ctacatgcat gacgacgcgc accggccgcg cttcttcgcc tatctctcct
      421 tcttcacctt cgcgatgctg gcgctcgtca catcgaacaa tttcctgcag ctcttcttcg
      481 gctgggaggg cgtggggctt tcctcgtacc tgctcatcgg tttctggtac acgcggccag
      541 ccgccaacgc cgcggcgatc aaggcttttg tcgtcaaccg ggtcggggat ttcggcttcg
      601 cgctgggcat cttcggggtc tacgcgatct ttcacacgct cgacttctcc tccgtgttcg
      661 ccgccgcgcc cgcgatggcg ggaaagacct tcctcttcgc cggccaccag ctcgacatcc
      721 tgacgacgct ttgcctgctt ctgttcatgg gcgcgatggg caagtccgcg caaatcggcc
      781 ttcacacctg gctgcccgat gcgatggagg gcccgacacc cgtctcggcg ctgatccacg
      841 ccgcgaccat ggtcacggcc ggggtgtttc tggtctgccg atgctcgccc atgttcgaac
      901 acgcgcccat agcgctcgcc ttcgtcacct tcatcggcgc gacgacggcg ttcgtcacgg
      961 cttcgatcgg cctgttccag aacgacatca agcgggtcat cgcctactcg acctgttcgc
     1021 agctcggcta catgttcgcg gccgctggcg tctccgccta cggcgctgcg atgttccatc
     1081 tcttcaccca cgccttcttc aaggcgctgc tgtttcttgg cgcgggctcg gtaatccatg
     1141 cgatgcatca tgagcaggac atgcgcagga tgggcgggct tgcaccgatg ctgccattca
     1201 cctgggcgat gatgctggtc ggcaatcttg cactgaccgg ttttggcctt ccgttcacgc
     1261 ctatcggcac ggcgggattc tattcgaagg acgcaatcat caacgcggct ttcctccacg
     1321 gtacgccgat ggcgacctac gcgttctggc tcacgctgac tgccgcgctg atgacgtcgt
     1381 tctattcctg gcgccagttc ctgatgacct ttcacggctc ctggcgcggc gcgcttgaag
     1441 agaaatatgc cagcgcggaa gacgatccgc atatgtacca cgaaccgctg gcaggcgcgg
     1501 atcgcggcca cgcgctccgc ttgtccgagg tgcgtgaatc gccgctgacg atgcttgttc
     1561 ccctcctcgt gctcgccata ggcgcgctga tcgcgggcgc gttgtttgcg catgacttca
     1621 tcggcgcgga cacggctggt ttctggcacg gcacaattgc cgccgtcacc ggccatgcgg
     1681 gcgccgcggc gacgcttcct ctgtgggtcg aagttgcgcc gttcgttctg acgattctcg
     1741 gttttgcaat tgcgtattac tactacgtgc tgcatccgga gctaccgccg cagatggcgc
     1801 agcggggcgg gcgtgtctac acctttctct acaacaaatg gtatttcgac gagctctacg
     1861 acgcggtgtt cgtgcgaccg gctctctgga tcgggcggct gctctggaag ggcggagacg
     1921 aacgcgtcat cgacggcatt ggccctgacg gcgtcgcggc gcgagtaata gacgccacgc
     1981 gggaagccgt gcgcctgcaa tcgggctacg tctatcatta cgcgtttgcg atgctggtcg
     2041 gcgtcgtggt gttggcgacc tggttcatgt tcaccgtggg cgccagatga gccatctgcc
     2101 gctcctcagc gtcgtgaact tcctcccgct tgcgggtgcc ctggtcattc tcgtgacacg
     2161 cggcagccgc gaaacgcagg cgcgcagtgc ccgcgccaca gctctcgccg ccacggtggt
     2221 aaacttggcg gtcgcgcttt tcatctggtc gcgcttcgac ggttcgacgg ccgcgttcca
     2281 gttcgtcgag caggcggtct ggctcggcaa tggcatcagc tatcgcgtcg gtgtcgacgg
     2341 aatctcgctg ctgttcgtgg tgctcaccgc cgggctcatg ccgttctgca tcctggcgag
     2401 ctggaaatcg gtcactgagc gtgtgcccga atacatgatc gccttcctgt tgctcgagac
     2461 gatgatgatc ggggtcttca cctcgctcaa tctcgtcctc ttctacgtgt tcttcgaagg
     2521 cggcctcgtg ccgatgttcc tcatcatcgg catctggggc ggcaatcgcc gcgtctatgc
     2581 gagctttaag ttctttctct acacgctgct cggctcgctg ctgatgctgc tcgcgatcat
     2641 ctcgatgtat ctcgagacag gcaccagcga catcgacgtc ctgatccgca ctgcgcattt
     2701 ctccgtcgcc atgcagacct ggctgtggct cgcctttttc gcctccttcg cagtgaaaat
     2761 gccgatgtgg ccggtgcaca cctggctgcc ggacgcccat gtcgaggcgc cgaccgcggg
     2821 ctccgtcatt ctcgccggca tcctgctcaa gatgggcggc tacggcttcc tgcgcttttc
     2881 gctgcccatg ctgcccaacg cctccgaggc gtttgcgccg ctggtcttcg tgctctccgt
     2941 cgtcgcggtc atctacacct cgctcgtcgc gctggcgcag gaagacatga agaagctgat
     3001 cgcctattcc tccgtcgccc atatgggtct tgtcaccatg ggcatcttct ctttgaccca
     3061 gcaaggcatc gacggcggaa ttttccagat gctgtcgcac ggcatcattt cgggggcgct
     3121 ctttctctgc gtgggcgtcg tctatgaccg catgcacacg cgcgagatcg gcgcctatgg
     3181 ggggctggtc gagcgcatgc ccgtctatgc cgtctgcttc atggtgttca cactcgccaa
     3241 tatggggctt cccggcacga gcggattcat cggcgagttc ctgaccatgc tggggacgtt
     3301 ccaggtcaac acctgggtcg ttattttcgc cgcgacgggc accattctgt cggcggccta
     3361 catgctgtgg ctgtacggcc gcatcatttt tggcgcgttg gtcaaaccgg cgctgacggc
     3421 gatccaggat ttgagcgcgc gcgagatcgc gatcttcacg cccctcgtcg tgctgacgat
     3481 cgccttcggc gtttacccca aaccgatctt cgatgtgacg tcggcatcgg tggccaacct
     3541 cgtcgcccag cacaagctcg cgatgatgaa ccgcggcaag ccggccgatc acctccatct
     3601 ggcttttctc aatatgccgg cgaaggatcc acgcggagac acggagacgc ggagaaaagg
     3661 ctcacaggga gccagtgagg cagtgagaca agaatcgcgc gcctttggcg cgcaatcgtt
     3721 ctcactgtct caccgtctca ttgtgaattc ttctcgttcc gcgccctccg cgcctccgcg
     3781 tgcccatcct tgggaaccac caatcggggc ggatagcagc tcgtcgtatg aggacgtgcg
     3841 atgagcggga tatcgagcga cctgctggtc ctgcttcccg agtgtctgct cgcgctcgcg
     3901 gcgatggcgc ttttgctgat cggggtgctt tcgacaaagc acgcgtttcg catcgtctcg
     3961 acggcggcga tcttcgcgct cgcggtggcg gcctggtttg tcgtctcgcc tgtctctgcg
     4021 agcccgagcg ccttccatgg cgccttcgtc gccgacggct tcgcgcgctt cgccaagctg
     4081 ctggtgctgc taggttctgc cctcaccatt cttatgtcgc aggaatttct cgagcgcgag
     4141 aagatcgcga acttcgaatt ccccgtcctc gtgctgctcg caacgatcgg tatgctcttc
     4201 atggtttcgg cggcgagctt catcgcactc tatctgggct tcgaactgca gagcctgtcg
     4261 ctctacgtgc tcgcggcctt caaccgcgat tcgctgcgct ccagcgaagc gggcctcaaa
     4321 tacttcgttc tgggcgcgct ttcctccggc atgatgctgt acggcatctc cttcatctac
     4381 ggctttgccg gcacgacggc ctttcccgaa gtggcgcgcg ccgtcacgca tgccggcgcc
     4441 aatatcggca tgatctttgg cctcgtcttc cttatcgcgg gactcgcctt caaagtttca
     4501 gccgtgccgt ttcacatgtg gacgcccgac gtctatgaag gctcgcccac gcccatcact
     4561 gccttcttct ccatagcccc gaagacggcg gcgatggcgt tgttcctgcg cgtgatgatc
     4621 gaggcgatgc ccggcgcgat ccacgaatgg caacagatca tcgtcttcgt ttccgtgctc
     4681 tcgatgttcc tcggttcggt ggcggcgatc gggcagggca acatcaagcg tctgatggct
     4741 tacagctcca tcgcccatat gggttacgcg ctgatcggca tcgctgcggg cacgtttcag
     4801 ggcgtggagg gcgtgctgct ttatctgctc atctatgtga tcaccaacgc gggcgttttc
     4861 gtctgcatcc tggcgatgcg ccggaacggg cagatggtgg agaacattcc cgatctcgcc
     4921 ggcctcgcgc ggaccaatcc gcggctcgcg cttgcctttg cggtgctgtt cgtgagcctt
     4981 gccggcattc cgcccttcgc cggtttctgg gcgaaattct atgtgtttct tgccgcgatc
     5041 ggggcgcatc tctacgcgct cgcgatcctg ggcgtgctcg caagcgcgat cggcgcttat
     5101 tattacctgc gcatcgtcaa gatcatgtat ttcgacgagc cggcccccgc cttcgatcgt
     5161 gaaatgggct tgcgtctggg cggcatcgcg gtgggggcga gcctgttcaa tctggtcttc
     5221 atcgtcatcg cctctccgct cgtcgccgcc gccgcgaacg cggctaagac gctcattccg
     5281 tgacggagat ttccgcgagc cttcggggca ttccgctccg ccatttcaag gaaatcgatt
     5341 ccacgaacga agaagcgcga agactggctc ttggcggagt cgagggaccg ctctggatca
     5401 ccgccgatcg gcagaccaaa ggccgcggtc ggcggggcag ggattggcaa tcgccggaag
     5461 gcaatctggc agccacgctg ctgctgaggc cggcaacgcc gatcgcgcaa tgcgcgcagc
     5521 tctctttcgt cgctgcgctc ggcgtgagcg aattcgtggg ccgctgcgcc ccgcgcgcgg
     5581 aagtgaaggt gaaatggcca aacgacgttc tggcggatga gaagaagatc gcgggcatcc
     5641 ttctcgaatc ggccagcaag gatggtgcgc gaccggattg gctcgcgatc ggcttcggca
     5701 tcaatctggc cttgcatccc gcggacaccg aatttccggc aacctcgatc gcaggcctcg
     5761 gcgagaaacc gcccgccccg cgtgttgcac tcgactgcct cgcggcctcg tggtccagat
     5821 ggtatgaaat gtggttcgaa aggggattcg agccgatccg tgacgcttgg ctcgcgcgcg
     5881 ccgcgaggct gggcgcacgc attcgggcgc ggatcgccga catggaggcg gtaggggtgt
     5941 tcgaaagtat cgatgccgat ggcgcgctga tcctgcgcga gagcaacggt cgcgcgcgtt
     6001 tgatctcggc aggggaggtg ttcttttgat gtggcgcccc tcacccacgc ctgagcggaa
     6061 ttcgacgctc gcacctgcat ccctctccca caaggggaga ggagaaatcg gaaccttgca
     6121 tcggcatcga agaatcaatg cgcggagttc cctctcccct tgtgggagag gggttaggcg
     6181 cgaggactct cttccgctca ggcgcgggtg agggggccac gtgctgctcg cgatcgatgc
     6241 cgggaatacc aacatcgtct tcgccatttg cgagggccgg gagattcgtg ccgaatggcg
     6301 cgcggtgatg gaccaggcac gcaccgccga cgaatacgcc gtgctcctga acccgctcct
     6361 gtcgcttgcc ggcttgaagt ttgccgatct cgactgcgcc atcatcgcga cggtcgtgcc
     6421 tgccgcgctg ttcgatctcc gcctgttctg caagcgctat cttcactgcg aaccgcttgt
     6481 cgtgggcgag cccgatgtcg atctcggcat cgagattcgg gtcgaccggc cgaacgcggt
     6541 gggcgcggac cggctcgtca acaccgtggc ggcccatgag cgctacaagg gagcgctgat
     6601 cgtcgtcgat ttcggcacgg cgacgacttt cgatgtcgtc agcgccgtcg gcaattacga
     6661 aggcggcgtg atcgcaccgg gcgtgaacgt ctccgccgag gcgctgcatc tgggtgccgc
     6721 gatgctgccg cgcgtcgcca tccagcgttc ccaatcggtg atcggcaagg acaccgtgcc
     6781 cgcgatgcag tcgggcctct attggggcta tctcggcctt gtcgaaggtc tcgtcgcgcg
     6841 catccgcgcc gagtatgggg aacccatgac ggtaatcggt accggcggtc tcgccggcct
     6901 cttctacagg cagaccgggg cgatcgagca tctggacgag gatttgacca tccgcggcct
     6961 cgtgcttatt cacgcgcgga acaccaacgc cggcccggcg cgtttgcacc gctgatggat
     7021 gcacgcctga acgaaagcga gcttgtcctg ctgccgctcg gcggcgcggg cgagatcggc
     7081 atgaacttca acgcctatgg ctgcggagcg cccgacgacc ggcgctggat cgtggtcgat
     7141 tgcggggtga tgttcggccg ggaaagcgcg acaccaggcg tcgacctgat cctgcccgat
     7201 atccgatttc tcgctgagca gcgcgagaac gtgctcgcca tcgtgctgac gcacgcgcac
     7261 gaggaccata tcggggcaat cgcccagctc tggccgctgc tgcgctgccc cctctatgcg
     7321 acacctttca ccgccaggct gatcgaaaac aagctcgcgg aggccggtct cgccgagcgc
     7381 gtgcgggtga ccgtcgttcc gctcggcggc cggatcgcga tcggtccctt cgagatcgac
     7441 ttcatctcca tcacccattc gatccccgag cccaacgcgc tcgcgatccg cacgcctttc
     7501 ggcgtcgtgg tgcacacggg agactggaaa ctcgaccccg atccgctcat cggcgaagtc
     7561 acggattccg agtccctgcg cgccttgggc gatgacggcg cgctggcgct tgtctgcgat
     7621 tccaccaacg cgctggtcgc gggcgagtcg ggatccgagg cgcgcgtgcg gacgagcctc
     7681 gtcgatctga tcggaaccct gaccggccgc gtcgccgtca ccgcgttcgc atcgaacgtc
     7741 gcgcggctcg aatccgtcgg caaggcggcg cgcacgcacg gacgctcgct ggcgctggtc
     7801 ggacgatcga tgaatcgtgt cgtcgaagcg gcgcgcgaga ctggttacct tcatgacttt
     7861 ccgccgctga tcgacgcaga cgaagcggcg caattgccgc cgaaccgcgt tctctatctc
     7921 tgcaccggca gccagggaga gacacgcgcg gcgctgtcgc gtgtcgcggc gggagaacat
     7981 cccactgtcg ctctcggacc cggcgatacg gtgatctttt cctcgcgtat tattcccggc
     8041 aacgatctcg ccatcttcga ggtgcaaaac aaactgtcgg cgctgggcgt tgaagtgctc
     8101 accgagcgcg atcatttcgt gcatgtctcc ggccatccct gtcgcgacga gctggcgcag
     8161 atgtatcgct gggtgcggcc gaaaatcgcc gtaccggtgc acggcgaatt gcgccacatg
     8221 acggaacatg cccgcctcgc gcgttcgctg caggtgccgc aggccatcgt gctgcacaac
     8281 gggcatatgc tgcggctggc gcccggccgc gccgagattg tcgacgagac gccgtcgggg
     8341 cgcatgcatc tcgacggcaa ggtgctggtc gaggaaggca aggggcatgc gcgcgcccgt
     8401 ggcgcgctcg gctatgcggg acttgtggcg gcgacgattg ctctggacca caagggacgc
     8461 gtggcgggcg aaccgtcgct gatcctcgag ggcattcccg accaggtgca cggcgcgatc
     8521 cgtacggcgc tcgcggatgt catcaagcgg catgaatcga agcggcgcga tgagcaggcg
     8581 ctcaaggaac aggcgcgcct cgccatccgc cgtgccgcaa tggcccactg gggaaagaag
     8641 cccgtcacgc gtgtcgaagt ggtctgggtg tgagaacgcg aagatgatcg gccggctcaa
     8701 ccacgtcgcc atcgcgacga aagacattgc ggccgccgcg aaagtctatc gcgacacact
     8761 gggcgcgaaa gtttctgagc cggttgcgca gccggatcac ggtgtcacca ccatcttcgt
     8821 cgagcttccc aacagcaaga tcgaactgat cgagccattg ggcgcggcgt cgccgatcgc
     8881 gaatttcctg cgaaaaaatc ccgcgggcgg aatccatcac atctgctacg aggtggacga
     8941 catcatcgcc gcccgcgacc gcatgcgcgc ggcgggcgcc acgatcactg gaaccggcga
     9001 accccgcatc ggcgcccacg gcaagccggt gatcttcctg cacccgaagg atttcgtcgg
     9061 caccctcatc gagctcgaac aggcgtgaga ctcgtcgttc tcgccggcgc gttcgtcatc
     9121 ctctggttcc tcgcgctgca tatcctgctg ccgatcggca tttcgccatc acacgacggc
     9181 gtcggcgatc cgggcgcgcc ggccaatccg cgcatggcgc tgaagctcgc gctggcgacc
     9241 gtcgcggcgg cggcgatgtg ggcgattttc tatgtgctcg tgttgttgcg agtgatcaat
     9301 atttgatttc gctttctcct taattgtcat ggcccgcaca agcgggccac ccagatggaa
     9361 gcggcaaaat tgtggaagtg cccgaagggc catcgcgcga ttacgcgatg gatcgcgtgt
     9421 ttcgactgag tggcccgctt gcgcgggcca tgccatggaa aaatagtggg agcagagaga
     9481 tgcaccgctt cacgccctcg ctgtttcctg ctccgcgagc gcgcctgttt cttccagggt
     9541 tttggtgagc ttcgcgtaat cggtcttgcc ggtgccgatg acgggaatgc tttcgaccag
     9601 cacgacgcgc cgcgggatgg cgagttcctg gacaccgtga ttgtgcaccc agccggcaaa
     9661 ctgcgtgcgt tcggcatccg ggcaggtcgt gacgagcagg acttcctcgc ccttgcgccc
     9721 gccgggtacg gagagcgccg catggctgta gtcgggccac atggcggacg cgcaactctc
     9781 gaccacagcg agcgaaacca tctcgccacc gatcttggcg aaacgcttga gcctcccctt
     9841 gatggcgatg aatccgtcag gatcgatgct gacgacgtca cccgtgtcgt accagccgcc
     9901 ggggggcgga gcgaaggtgc ccggctgctc cggcctgatg taacccagca tcacgttcgg
     9961 cccgcgcacg acgaggcgcc cggcatgcgg aatgccctcg accggctcga ggcggctttc
    10021 gattccctgc agcaggcgtc ccaccgtgcc gggccgattc ataccccgct ggttggcggc
    10081 gatcacgggc gcggcttcgg tgacaccata accttcgagc agctcgatgc tgtatttttt
    10141 gcgcaccagc gtgcgcgtct cgtcgcgcag gcgttcggcg ccgcagaccg cgagcctgag
    10201 actgttcaga tcgccctggt cgcccgcgcg cgccgtttgc gagatgaacg tgtcggtcga
    10261 gagcaggatc gtcgcgccgg attggcggat gcggcgcgca atctcgcgcg gctgcagcgg
    10321 cgtgggatgg aacaccgcct tgattcccag cagtaacggc atcagcgcac cgaccgtcag
    10381 cccgaagcag tggaaggtcg gcaacgggtt gaacaggacg tcgtgggtat atagctcgat
    10441 gtg
//
LOCUS       DASZMC010000104         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2530147,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000104 DASZMC010000000
VERSION     DASZMC010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530147"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..760
                     /gene="serS"
                     /locus_tag="VG274_01960"
     CDS             11..760
                     /gene="serS"
                     /locus_tag="VG274_01960"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWE05442.1"
                     /translation="MQFMLDLHTGEHGYEEVNPPVLVRDAAMFGTAQLPKFRDDQFAA
                     GDGFWLIPTAEVPLTNLVRESIVDEKRLPLRYTAGTLCFRAEAGAAGRDTRGMIRQHQ
                     FEKVELVSITTPEQSAAEHERMTACAEEVLKRLGLHFRTMLLCAGDMGFAARKTYDIE
                     VWLPGQGRFREISSCSVCGDFQARRMGTRCKAADAKSTRFVHTLNGSGVAIGRALVAV
                     LENYQEPDGGVSIPEALWPYMGGLKRIERAG"
     gene            902..2053
                     /locus_tag="VG274_01965"
     CDS             902..2053
                     /locus_tag="VG274_01965"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWE05443.1"
                     /translation="MFYASVGPELTFYHVDADGIELSRQDSLTLPANVQYAWPHPSAN
                     ILYATWSNGGPGIAGDKHGASALSIDPSNGALRPHGPSAALRSRPIHISIDGSAGHAL
                     IAHNDPSGITVLRINEDGSIGEEVKQRTAPDVGIFAHQIRVMPSGKAVILVTRGNDRA
                     AGKPEDPGALKIFSFEDGQLTNRASIAPGGGYGFGPRHLDFSPAQPWVYVSLERQNKL
                     DVFRIQNDSLDPQPIFSKNTLAEPGNLRPRQLAGAVHVHPNGQYVYVANRSDAMTDFQ
                     GRQVYLGGENNIAVFAIDAGTGEPALIQTADSHGFHPRTFSLHPSGPMLVAANITSRL
                     VRDGDQLRLQPATLAAYRVCADGRLSFARSYDVETGGGYQFWSGMVALS"
     gene            2215..2499
                     /locus_tag="VG274_01970"
     CDS             2215..2499
                     /locus_tag="VG274_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05444.1"
                     /translation="MSDVAEKMQISLRAPTATIDAFDRIAKALDRDRTWVMLQAFSAY
                     LKGEGGHILEEAEGIAELERGESVDFDEAMDEVDKIISDSEQAAVAKRSA"
     gene            2499..2804
                     /locus_tag="VG274_01975"
     CDS             2499..2804
                     /locus_tag="VG274_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWE05445.1"
                     /translation="MAKRCARLSKRAQSWLEREIRYIAVRSPPAAKKLAMRIREARIL
                     LTEHPKSAQAGQIHGTRRLVIPPYILTVMQRGGTVEIVAIRHAQQGDAYVPEELSDD"
     gene            complement(2859..>3074)
                     /locus_tag="VG274_01980"
     CDS             complement(2859..>3074)
                     /locus_tag="VG274_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05446.1"
                     /translation="VADLGRVRLSGFPGWLFWSLVHLFLLAGFRNRLVVYVNWSWAWF
                     THGRSARLIVGTPPREEMEGPRHHSGR"
BASE COUNT          617 a          945 c          958 g          554 t
ORIGIN      
        1 gcgcgcgctg atgcagttca tgctcgatct gcacacgggc gaacatggtt acgaagaggt
       61 taatcccccg gtgctggtgc gcgacgctgc aatgttcggc accgcgcaat tgccgaagtt
      121 tcgcgacgac cagttcgccg ctggcgatgg cttctggctg attccgaccg cggaagtccc
      181 gctcaccaac ctcgtgcgcg agagcatcgt cgacgagaag cgcctgccgc tgcgctatac
      241 cgccggcacg ctctgctttc gtgcggaggc gggcgcggcg gggcgcgaca cccgcggcat
      301 gattcgccag caccagttcg agaaggtcga actggtctcg atcacgacgc cggaacaatc
      361 cgccgccgag cacgagcgga tgaccgcctg cgccgaggag gtgctgaagc ggctcggtct
      421 gcatttccgc acaatgctcc tctgcgcggg cgacatgggc tttgccgccc gcaagaccta
      481 tgacatcgag gtctggctgc cggggcaggg gcgtttccgc gaaatctcgt cctgctcggt
      541 ctgcggcgat ttccaggccc ggcggatggg cacgcggtgc aaggccgccg acgccaaatc
      601 gacccgcttc gtccacacgc tcaacggctc gggcgtcgcg atcggccgcg ccctggtcgc
      661 ggtgctggag aattatcagg agccggacgg cggcgtctcg atccccgagg cgctatggcc
      721 ctacatggga gggttgaagc ggatcgagcg ggcggggtga ttgttcgtac tcgcttgtgg
      781 cggcagccgc gccgcgcgca tgagaggatg ccctcatgtt ggatcgccgg atgttcagcg
      841 actcctggct gccgcgatca ccgcgccccg ggcctgcttg gctcaaccaa agggtgcaac
      901 catgttctac gccagcgttg ggcccgagct cactttctac cacgtcgatg cggacggcat
      961 cgagctctcg cggcaggatt ccctaacgct acccgcgaac gtccaatacg cttggccgca
     1021 tccttcagcc aacattctct acgcgacgtg gagcaacggc ggtcctggta tcgcgggcga
     1081 caagcacggc gccagcgcgt tgagcataga cccgagcaac ggcgcgcttc ggccacatgg
     1141 accttctgct gcgttgcgat cgcggccgat acacatcagt attgacggat ccgccggcca
     1201 tgcgctgatc gcccacaacg acccgagtgg catcaccgta ctccgaatca acgaggatgg
     1261 gagtatcggc gaggaggtga agcagagaac agctcccgat gtcgggatat ttgctcacca
     1321 gatccgcgta atgccatctg gcaaagcggt gattctcgtt acgcgaggca acgatcgcgc
     1381 tgccggcaag ccggaagatc ctggcgcgct gaagattttc agtttcgaag acggccaact
     1441 cacgaaccgg gcatcgatcg ccccgggagg tggttacggc ttcggaccaa gacacctcga
     1501 cttttccccg gcgcagccct gggtgtacgt ctcgctcgag cggcagaaca agctcgatgt
     1561 cttcaggata cagaacgaca gcctcgatcc gcaacccatt ttcagcaaga acactttggc
     1621 cgagccgggc aatctccggc ctcgacagct cgcgggggcg gttcatgtgc acccaaacgg
     1681 gcagtacgtc tatgtcgcca atcgctccga cgcgatgacc gatttccaag gcaggcaggt
     1741 ttacctcggt ggcgaaaaca acatcgccgt atttgcgatc gacgccggaa cgggcgagcc
     1801 ggcacttatt cagaccgcgg attcgcatgg gttccacccg cgaaccttct cgctccatcc
     1861 gagcggaccc atgctggtcg ccgccaacat cacctcgcgc ctggtgcgtg acggagatca
     1921 actgcgactg cagccggcaa cgctggccgc ctatcgcgtc tgcgccgacg gcaggctctc
     1981 gttcgcacgc tcctatgatg tcgagaccgg tggcgggtat cagttttgga gcggaatggt
     2041 ggcgttgagc tagcgggcga agaccgattg gcttgtcgct tcgcgcggcc tcattgccgc
     2101 aaggcgccag ggtcttccag actacggtgc attgccgtaa cgccaagaag gactttgatt
     2161 gcgcaagaca acgtaatatg ttaaatgacg gtgtcatacg acagaggagt tcccatgagc
     2221 gacgtggccg aaaagatgca gatatcgctt cgcgcgccca ctgcgacgat cgatgctttt
     2281 gatagaatcg cgaaagctct cgaccgggat cgcacatggg tgatgttgca agcgttttcc
     2341 gcatacctga aaggcgaagg cggccatatt ctcgaagagg cggaaggaat tgcagagctc
     2401 gagcgtggag agagcgtcga tttcgacgaa gccatggatg aagtcgacaa aattatctcc
     2461 gactcggagc aggccgctgt tgcgaagaga tccgcctgat ggccaagcgc tgcgcgcgcc
     2521 tctcgaagcg cgcgcagagt tggctggagc gggagatcag atatatcgcc gtcagaagcc
     2581 ctcccgctgc caaaaagctc gcgatgcgca ttcgggaggc ccgaatcctt ctgaccgagc
     2641 atccgaagag cgctcaggcc ggccaaattc atggaacacg gcgcttagtt attccaccgt
     2701 acatactgac agtgatgcag aggggcggaa cagtagaaat tgttgcgata cgacacgcgc
     2761 agcagggcga cgcctatgtt ccggaagaat tgtcggacga ctagtcgtct tctccgaaga
     2821 tgcgagcgcg cgaaaactcg gattggcggt ttcgccgctc atcgtccaga atgatgtctt
     2881 ggcccctcca tttcctcgcg gggcggtgtg cctactatca gccgcgcgct ccggccgtga
     2941 gtgaaccacg cccatgacca attgacgtaa accacgagcc ggttgcgaaa tcccgccagg
     3001 agaaagaggt ggacgagcga ccagaacagc cagccgggga agccgctcag ccgcacgcgg
     3061 cctagatccg cgac
//
LOCUS       DASZMC010000105         5914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2530930,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000105 DASZMC010000000
VERSION     DASZMC010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530930"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1039
                     /locus_tag="VG274_01985"
     CDS             302..1039
                     /locus_tag="VG274_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWE05447.1"
                     /translation="MQLHLSSWPEIEAYLANSKAILVPIGSTEQHGPTGLLGTDALCA
                     EFIGKRAGEEAGFLVAPTFNVGQAQHHMAFPGTMTLRPSTMIAAMVDWARSLTHHGFT
                     RLFWLNGHGGNIATINAGFSEIYHAASLEGGARVLCKLRNWWELPGVGDVCRQLFPVG
                     EGHHATPSEVSVTYYMHPQAQKQAKMEPKIAPLGPIHDAADYRRRFPDGRIGSDPSQA
                     SPEAGEKIVTAAVKAVITEFRQFTESE"
     gene            complement(1203..3113)
                     /locus_tag="VG274_01990"
     CDS             complement(1203..3113)
                     /locus_tag="VG274_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05448.1"
                     /translation="MLESGLRARGTRLVLALAMLTAAVATAGAKPVDVLTYHYDNLRT
                     GWNSNEKKLTPANVGSAQFQLLATTTLDEQVDAQPLVLASESVNGNSAREVVYIATEG
                     DTVYAIDANTGSILAQRNLGTPVPRTSLPGQCTNGGPNLGINATPVMDPTTHTLYLLA
                     YLYVNQQPVWQVYAIDPSTLTDAVAPVVVEAHHRLDDHTRYDFDPHEARNRAGLLLAN
                     GNLYAGFASFCDYDPNLTRGWVLGWQAATLTPLRGKEFTNKLSQSTDDWFMTSIWMSG
                     YGIAGSAAGDVYFTTGNSDPAGNSYNNRKNISESVVQMPSDLSSVKHLYTPMGQYGWQ
                     YLDETDGDFASAGIMLLPPQNGQPSNLAVSGAKAGIYYVFNADDLTNGQRNGGKEYSN
                     INVGPCWCGPSYYMGSDGVARIVTSSNNTVGVYKLKATGTPTLTLDKNVGSVEGAFFP
                     GFLTSVSSDGSTPGTTVIWAVGRPTDFNQEALYLHAYDAENGGELFKTLAGYWTNSFG
                     DSNIVPVVDNGKVYVATVKSLAIYGLKTGGARVAALVPPKILERPPLAPGQHEIRGTV
                     RRITGAVLLVEKRDGKTITVDAARAVANGKAAPPSVGHALVARGTMEGDVLKAELIGH
                     VPGHVEVWPPDR"
     regulatory      3231..3338
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3330..5159
                     /gene="thiC"
                     /locus_tag="VG274_01995"
     CDS             3330..5159
                     /gene="thiC"
                     /locus_tag="VG274_01995"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HWE05449.1"
                     /translation="MNKPILNPRPVTNDVVTRGSLPGSKKVHVDGVPFREVALSGGEA
                     PMRLYDTSGPYTDECAPIDIEKGLAPRRREWIVTRGDVEEYEGRARRPEDDGLKRGEL
                     LSVPQFDRGARKCLRAKPERNVTQLHYARQGLVTEEMKFIAARENLGRSQFRDGESFG
                     AAIPDEITPEFVRSEVARGRAIIPVNINHPESEPMIIGRNFLTKVNANIGNSIVTSGA
                     AEEVEKLVWAIRWGADTVMDLSTGRHIHTIREWIVRNAPVPIGTVPIYQALEKVGGVA
                     EELTWEIYRDTLVEQCEQGVDYFTVHAGVRLAHIPLTAERVTGIVSRGGSILAKWCLA
                     HHRENFLYTHFEEICELLARYDVSFSLGDGLRPGSIADANDAAQFAELETLGELNRIA
                     QKHDVQVMIEGPGHVPMHRIRENMERQLAVCDEAPFYTLGPLTTDVAPGYDHITSAIG
                     AAMIGWFGTAMLCYVTPKEHLGLPDRDDVKAGVIAYRIAAHAADLAKGHPAARLWDDA
                     MSKARFEFRWRDQFALALDPETAQAFHDETLPADGAKVAHFCSMCGPKFCSMKISQEV
                     RDAARGANDTLSSAEIRAAMEKKSQEFRARGSEIYVTTSVREL"
     gene            complement(5452..>5914)
                     /locus_tag="VG274_02000"
     CDS             complement(5452..>5914)
                     /locus_tag="VG274_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HWE05450.1"
                     /translation="VAEEQFMANGPPPKAFVKSAVAVHNFLFRISGGRIGSRFAGAPV
                     LLLSAKGRKSGVTRTVPVLYVTTDKGFALVASFAGAQKHPDWYLNLDAAGVADVQIRD
                     RRMRVRAETVDPDSERYRTIWSEAAALYPDYDTYQTRTTRKIPIVELIPLT"
BASE COUNT         1048 a         1863 c         1966 g         1037 t
ORIGIN      
        1 ccggcgcgga gtttatcctc cggccgcgct tcgcgcggac cggggggcgc caccatctcc
       61 cacaggggga gaagggaatt gttgatgcag cgcgcggaat cccctctccc cttgtgggag
      121 aggcgtgcag atacgggcct cacaaccact caagcgtggg tgaggggtag acgagcgcga
      181 tacggcggcg tttggcccta tccggacccg caaagcctcc gctaagcttc gctggcctga
      241 ggagaagcgc cgcgttccgg aaggcattgg agaagttgga cataaaagga acgttcattc
      301 catgcagctg catctttcct cgtggcccga gatcgaggct tatctggcga attcgaaagc
      361 catcctcgtt ccgatcggct cgacggagca gcacgggccg accgggcttc tgggcaccga
      421 cgcgctgtgc gcggagttca tcggcaagcg ggcgggcgag gaagcggggt ttctggtcgc
      481 gccgaccttc aatgtcgggc aggcgcagca ccacatggcg tttcccggaa cgatgacgct
      541 gcgtccctcc accatgatcg ccgcgatggt cgactgggcg cgctcgctca cccatcatgg
      601 tttcacccgc ctcttctggc tgaacggcca tggcggcaac atcgccacga tcaatgcggg
      661 tttctcggag atctatcacg cggcgagcct cgaaggcggc gcgcgcgttt tgtgcaaact
      721 gcgcaactgg tgggagctgc cgggcgttgg cgatgtgtgc cggcagctct ttccggtggg
      781 cgaggggcat cacgccacgc cctcggaagt ttcggtgaca tattacatgc acccgcaagc
      841 gcagaagcag gctaagatgg agccgaagat cgcgccgctc gggcccattc acgacgcggc
      901 cgattaccgc cgccgcttcc ccgacggccg catcggctcc gatcccagcc aggcgagccc
      961 ggaagcgggc gagaagatcg tgaccgccgc ggtcaaggcc gtgatcacgg aattccgcca
     1021 attcaccgag agtgaataga aaaaggattt aacgcagagg acgcagagga tgcgcagtgg
     1081 gcgcaaaata agactttgcg cgccttcggc gcgccaatct cgtttatccg cgtcgtccgc
     1141 ggaacctctg cgtcctctgc gttaaaactt tttgtcgtcg cgaccgaccg cggtccaccc
     1201 gctcagcggt cgggcggcca tacctccacg tgacccggca catggccgat cagctcggcc
     1261 ttcagcacgt cgccttccat cgtgccgcgg gccaccagcg cgtggccgac cgaaggcggc
     1321 gcagccttgc cattcgccac agccctggcc gcatcgaccg tgatggtctt gccgtcgcgc
     1381 ttctcgacga gaagcacggc gccggtgatg cggcgaaccg tgccgcggat ctcgtgctgt
     1441 cccggcgcga gcggcggacg ttcgagaatc ttcggcggca cgagcgccgc aacgcgcgcg
     1501 ccacccgtct tcaggccgta gatcgccagc gacttcacgg tcgcaacata gaccttgccg
     1561 ttatccacca ccggcacgat gttggaatcg ccgaaggaat tcgtccagta gccggccagc
     1621 gtcttgaaca gctcgccacc gttttcggcg tcataggcgt gaaggtagag cgcctcctga
     1681 ttgaaatccg tcggccgtcc caccgcccag atcacggtcg tgcccggagt cgagccgtcc
     1741 gaagacacgc tggtgaggaa tccgggaaag aacgcgccct ccacgctgcc cacattcttg
     1801 tcgagcgtga gagtcggcgt tccggtcgcc ttgagcttgt acacgcccac cgtgttgttg
     1861 ctgctcgtga cgatgcgggc gacgccgtcg ctgcccatgt agtaggacgg tccgcaccag
     1921 cagggcccga cattgatgtt gctgtactcc ttgccgccat tgcgctgacc gttggtgagg
     1981 tcgtcggcat tgaacacata atagatgccg gccttcgcgc ccgacaccgc gaggttgctg
     2041 ggttggccat tttgcggggg aagcagcatg attccagccg acgcgaaatc gccgtccgtc
     2101 tcgtcaaggt actgccatcc gtactgcccc atgggcgtgt agagatgctt cacggaggac
     2161 aaatccgacg gcatctgcac gacgctttcg gaaatgttct ttctgttgtt gtaggaattg
     2221 cccgcgggat cggaattgcc ggtggtgaag tacacatcgc ctgccgcgct gccggcgatg
     2281 ccatagccgg acatccagat cgaggtcatg aaccaatcgt cggtcgattg cgacagcttg
     2341 ttggtgaatt ccttgccgcg cagcggcgtc agcgtggcgg cctgccaccc cagcacccag
     2401 ccgcgcgtga ggttgggatc gtagtcgcag aagctcgcaa atcccgcgta gaggttgccg
     2461 ttggcgagca gaaggccggc gcgattgcgg gcctcatgcg gatcaaaatc gtacctcgtg
     2521 tgatcgtcga gcctgtggtg cgcctcgacg accacgggag cgacggcatc ggtaagcgtc
     2581 gacggatcga tcgcgtacac ttgccagacc ggctgctgat ttacatagag ataggcgagc
     2641 agatagaggg tgtgcgtcgt ggggtccatc accggcgtgg cgttgatgcc gagattgggc
     2701 ccgccgttcg tgcactggcc gggcagagac gtccgcggca ccggcgtgcc gaggttgcgt
     2761 tgcgcgagaa tgctgccggt gttcgcatcg atcgcataga cggtgtcgcc ttcggtcgcg
     2821 atgtacacga cctcgcgggc gctgttcccg ttcaccgact cgctggcgag gaccaaaggc
     2881 tgggcatcca cctgttcgtc cagcgtggtc gtggcgagca gctggaattg cgcgctgccg
     2941 acattcgcgg gcgtcagttt cttttcattg ctgttccagc ccgtccggag attgtcgtaa
     3001 tgataggtga ggacgtccac cggctttgcg ccggccgtcg caacggccgc agtcaacatt
     3061 gcgagcgcaa gtacaaggcg ggtgccgcgc gcccgtaaac ctgattccag catgtgagcc
     3121 cctccaaagg cgggccagtc tgcgcctggc ccggcggccg cgcaatacag gctgcgtgcc
     3181 gacaccctgc ccggcgtgaa gcttgacttg gccggggcgg ggcccaagat cggtcttccc
     3241 aggggcgccc gtgcgagcgg gctgagatcg cggaaggcgc gagtcccttt gaacctgatc
     3301 cggttcacac cggcgtaggg agaggcccaa tgaacaaacc catcctgaat ccccgcccgg
     3361 tcaccaacga tgtggtgacg cgcggcagcc tgccgggctc gaaaaaggtc catgtggacg
     3421 gcgtgccgtt ccgcgaagtg gcgctgtcgg gcggcgaggc gccgatgcgg ctctacgaca
     3481 cgtctggtcc gtacacggat gaatgcgcgc caatcgacat cgaaaagggg ctggcgccca
     3541 ggcggcgcga atggatcgtc acgcgcggcg acgtcgagga atacgagggc cgggcgcgcc
     3601 ggccggaaga tgacgggctg aagcgcggcg agctgctctc cgtgccgcaa ttcgaccgcg
     3661 gagcccgcaa gtgtctgcgc gccaagccgg agcgcaacgt tacgcagctg cactacgcgc
     3721 gccagggact cgtcaccgag gaaatgaaat tcatcgccgc gcgggagaat ctcggccgct
     3781 cgcaattccg cgacggcgaa agcttcggcg ccgcgatccc cgacgagatc acgccggaat
     3841 tcgtgcgcag cgaagtggcg cgcggccgcg ccatcatccc cgtgaacatc aaccatcccg
     3901 aatccgagcc gatgatcatc ggccgcaatt tccttaccaa ggtgaatgcg aatatcggca
     3961 attccatcgt cacctcgggt gcggcggagg aggtggagaa gctggtctgg gcgatccgct
     4021 ggggggcgga tacggtgatg gacctctcga ccgggcgcca catccacacc atccgcgaat
     4081 ggatcgtgcg caacgcgccg gtgccgatcg gcacggtgcc gatctaccag gcgctggaga
     4141 aggtgggcgg cgtcgccgag gaactgacgt gggaaatcta tcgcgacacg ctggtcgagc
     4201 aatgcgagca gggcgtggat tacttcaccg tgcacgcggg ggtgcggctc gcgcatatcc
     4261 cgctgacggc ggagcgcgtc accggcatcg tcagccgcgg cggctcgatc ctcgccaaat
     4321 ggtgcctcgc ccatcaccgc gagaatttcc tctacacgca tttcgaggag atttgcgaac
     4381 tgctcgcgcg ctacgacgtg tcgttttcgc tgggcgacgg attgcggccg ggctcgatcg
     4441 ccgatgcgaa cgacgccgcg cagttcgccg agctcgagac gctgggcgaa ttgaaccgca
     4501 tcgcgcagaa gcacgacgtg caggtgatga tcgagggccc cgggcacgtc ccgatgcacc
     4561 gcatcaggga gaacatggag cggcagctcg cggtctgcga cgaggcgccg ttctatacgc
     4621 tcgggccgct caccaccgac gtggcgcccg gctatgatca cattacgagc gcgatcggcg
     4681 cggcgatgat cggctggttc ggcaccgcca tgctctgcta cgtgacgccg aaggagcatc
     4741 tgggcctgcc cgaccgcgac gacgtgaagg cgggcgtgat cgcctaccgc atcgccgccc
     4801 atgcggccga tctcgccaag ggccatccgg cggcgcggct gtgggacgac gcgatgagca
     4861 aggcgcgctt cgagtttcgt tggcgcgacc agttcgcgct ggcgctcgat cccgaaaccg
     4921 cacaggcctt tcacgacgag acgctgccgg cggacggcgc gaaggtcgcc catttctgct
     4981 cgatgtgcgg gccgaaattc tgctcgatga aaatctcgca ggaagtgcgc gacgccgcgc
     5041 gcggcgcaaa cgacacgttg tcctccgccg agatccgcgc cgcgatggaa aaaaagtcgc
     5101 aggaattccg cgcgagaggt tcggaaattt atgtgacgac atctgttcgc gagttgtgaa
     5161 gcatcagcgc ctgccctttc ctcccccgca cttcgcgggg gaggtggcgc gagcggagcg
     5221 aagcgccgga gggggtaagt ggcgtgtccg ccgcttgccc cctccgcccc ttcggggcac
     5281 ctcccccacg caagggcgcg ggggaggaaa agaaagcgcg aatcaaggtt ttgaggcggt
     5341 gagacaggga ggcgtctttg cgcgccagcg gcgcgctatg ttctctcacc gccttaccgc
     5401 ctcattgcga atcttttctt ggcgtcctgg cgggtccacc tttttacccg atcaggtcag
     5461 cgggatgagt tcgacgatcg ggatcttccg cgtcgtgcgc gtctgatagg tgtcgtagtc
     5521 gggatagagc gcggcggctt cgctccagat tgtgcggtag cgctccgaat ccggatcgac
     5581 ggtctccgcg cgtacgcgca tgcggcggtc gcggatctgc acgtcggcga cgcccgcggc
     5641 gtcgaggttc aggtaccagt cgggatgctt ctgcgcgccg gcaaaggagg cgaccagcgc
     5701 gaagcccttg tcggtcgtca cgtagagaac cggcacggtg cgcgtgacgc ccgattttct
     5761 tcccttcgcg ctcagaagga gaaccggcgc tcccgcaaag cggctgccga tccgcccacc
     5821 gctgatgcgg aagaggaaat tgtggacggc gacggccgat ttgacgaagg ccttgggcgg
     5881 tgggccgttt gccatgaatt gctcctcagc gacg
//
LOCUS       DASZMC010000106         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3898764,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000106 DASZMC010000000
VERSION     DASZMC010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3898764"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VG274_02005"
     CDS             complement(<1..644)
                     /locus_tag="VG274_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HWE05451.1"
                     /translation="MSGPSTYEPKTNFTRWLDTRLPLLRFVNDTLTTFPTPRNLNFWY
                     SFGGILTFCLGIQIVTGIVLAMHYVSSSDLAFDSVENIMRDVNYGWLLRYMHANGASM
                     FFIAVYVHIFRGLYYGSYKAPREILWILGALIFVLMIATAFLGYTLPWGQMSFWAATV
                     ITNLFSAFDQIIPHLGTAITTWLWGGFGVSDATLNRFYSLHYLLPFVIAGLVVL"
     gene            complement(655..1209)
                     /gene="petA"
                     /locus_tag="VG274_02010"
     CDS             complement(655..1209)
                     /gene="petA"
                     /locus_tag="VG274_02010"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWE05452.1"
                     /translation="MADANAAEPTRRDFIYIATGAAGAVGVALAAWPFIDQMNPTGSV
                     LALSSIEVDLAPIQPGQQIVLTWRSHPLFVRRRTPREIAEARAVPVSALLDPLARNAN
                     LADDAPATDQNRETKSEWLILVGVCTHLGCTPTAFEGNYGGWLCHCHGSQYDVAGRVR
                     RGPAPRNLDVPKYTFLSGTRVKVG"
     gene            complement(1369..1785)
                     /locus_tag="VG274_02015"
     CDS             complement(1369..1785)
                     /locus_tag="VG274_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05453.1"
                     /translation="MRWPLLLDTCAAIWIMDNAELEPAAVEAMDQAFDCGESVYASPI
                     IGWEIGLLAWRGRFKSQLAPQRWLKEFLETPNVKLAEMPPGVLLESSLLPGTPPRDPA
                     DRIIAATAREFGLTVMTRDRALLDYASAGYLNAIPC"
     gene            complement(1782..>2001)
                     /locus_tag="VG274_02020"
     CDS             complement(1782..>2001)
                     /locus_tag="VG274_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05454.1"
                     /translation="VPRKETSPEHQNREVLESSRAFAPAEKKPRRHPAFGALKGTFTI
                     EPGYDLTQPAMPEWADMIDEKYGPEIRK"
BASE COUNT          401 a          598 c          642 g          360 t
ORIGIN      
        1 tgcagcacga caaggcccgc aatcacgaac ggcaggaggt aatgcaggct gtagaagcgg
       61 ttgagagtgg cgtcgctgac gccgaagccg ccccacagcc aggtggtgat cgcggtgccc
      121 agatggggaa tgatctggtc gaaggcgctg aacagattgg tgatgacggt ggcggcccag
      181 aacgacatct ggccccaggg cagcgtgtaa ccgagaaacg ccgtggcgat catcagcacg
      241 aaaatgaggg cgcccagaat ccacaggatc tcgcgcggcg ccttgtagga accgtagtaa
      301 aggccgcgga aaatgtggac gtagaccgca atgaagaaca tcgaggcgcc gttcgcatgc
      361 atgtagcgca gcagccaccc atagttcacg tcgcgcatga tgttttcgac gctgtcgaag
      421 gcaagatcgg aactggagac gtagtgcatc gcaagcacga tgccggtgac gatctggatg
      481 ccgaggcaga aggtgagaat gccgccgaac gaataccaga aattgaggtt ccgcggcgtc
      541 gggaacgtgg tcagcgtgtc gttgacgaag cgcaggagcg gcagacgcgt gtccagccag
      601 cgggtgaagt tcgtcttcgg ttcgtaggtc gacggtccgg acatttggcg ctcctcaccc
      661 gaccttcacg cgggtgccgg acaggaaggt gtatttcggc acgtccagat tgcgcggcgc
      721 gggcccacgg cgcacccgtc cggccacgtc atattgcgag ccgtggcagt gacagagcca
      781 tccgccgtaa ttgccttcga atgcggtcgg cgtgcatccc agatgcgtgc agacgcccac
      841 caggatcaac cactccgatt tcgtctcgcg gttctggtcg gtcgccggcg catcgtcggc
      901 aaggttggcg ttgcgcgcca gcggatcgag cagcgccgag acggggaccg cccgcgcttc
      961 cgcgatttcc ctgggcgtgc ggcggcgcac aaagagcggg tggctgcgcc aggtcaggac
     1021 aatttgctgc cccggctgga tcggcgcgag atcgacctcg atggacgaca gggcgaggac
     1081 ggagcccgtc ggattcatct gatcgatgaa cggccatgcg gccagcgcga cgcctacggc
     1141 gcccgccgcc ccggtcgcga tatagatgaa gtcgcgccgt gtgggctcgg ccgcattcgc
     1201 atctgccacg ggttcacctt ctgctgggga aatgaggcgc tccgggacga ggaagggaac
     1261 gcgccgcccc cggaatcgcc ggcgcccctt gaatccatgg cgcaaaatag agggtcaggt
     1321 ccagagggtc ttgcggcacg atgccgcata attccagtgg atttttcttc aacaggggat
     1381 cgcgttcaga tagccggcgc tggcgtaatc cagcagagcg cggtcgcgag tcatcactgt
     1441 caacccgaac tctcgcgccg ttgcagcaat gatccggtct gcaggatccc gcggcggcgt
     1501 tcccggcagg agcgaagatt cgagcaacac gcccggcggc atttcggcca gcttcacatt
     1561 cggtgtttcc aggaattctt tcagccagcg ctgtggcgca agttgagact tgaatcgtcc
     1621 gcgccatgcc agaagcccga tttcccaacc aatgatcgga gatgcataga cggactcgcc
     1681 gcaatcgaac gcttgatcca tcgcctccac cgctgcaggc tcgagctccg cattgtccat
     1741 gatccaaata gcggcacagg tatcaagcag cagcggccat ctcacttgcg aatctccggc
     1801 ccatatttct cgtcgatcat atctgcccat tcgggcatcg ctggctgcgt caaatcatat
     1861 cctggttcaa tcgtgaacgt gcccttcagc gcgccgaacg cgggatgacg gcgcggcttt
     1921 ttttccgctg gcgcaaatgc gcgcgaggat tccagaacct cccggttctg atgttcgggg
     1981 ctcgtttctt tgcgcggaac a
//
LOCUS       DASZMC010000107         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4188317,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000107 DASZMC010000000
VERSION     DASZMC010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4188317"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VG274_02025"
     CDS             <1..204
                     /locus_tag="VG274_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05455.1"
                     /translation="PDIDFAYEEIPCKTHAMGAKGCGEAGTVGALPVIISAICDAIGI
                     EHLDMPATPERVWRAIRDTKARA"
     gene            283..1179
                     /locus_tag="VG274_02030"
     CDS             283..1179
                     /locus_tag="VG274_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05456.1"
                     /translation="MNTAHSGSLVGFALLALAAPNAVFAADNIAAETVVVTATRNLDA
                     NTLGTLSSQFVDSRTALSPIRQIARWRAPVCPAVQGLPPAFNKFVSDRIKAIAVKVGA
                     PVDASCKSNIEVMFTPETQTMANDVADKTPWLFGTRLTTSAMHLATVNHPIQAWYVTA
                     TIDPTGQQRTDDPADPVGVTGSNRKISGSRLGANKTSVLENVLIIADAGKLAGHDIGT
                     VADYIALLALSQMQPSDDCAALPSVADAMAASCSKKPAPDSITAVDTAFLEGLYATQP
                     DVLGSIARADIARRTAQGLSGQ"
     gene            complement(1190..1366)
                     /locus_tag="VG274_02035"
     CDS             complement(1190..1366)
                     /locus_tag="VG274_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05457.1"
                     /translation="MRKSLLILAGIFAFAALWKKEADEDGVGIAASPFLAALVIDAAI
                     LLLSRKRNTLLSDA"
     gene            complement(1427..1963)
                     /locus_tag="VG274_02040"
     CDS             complement(1427..1963)
                     /locus_tag="VG274_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05458.1"
                     /translation="MYKAITLAAAVLLAACIPAAAIDFASPSADIRARNEFAIGVDLV
                     NHHKYLDATPHLETALQQFPDDTDILKYLGFAHRMIARQYSGTSHAGELRLSNTYYRR
                     ALDVDPDRKDFLEYMGELYLEMDDVAAAREKLAALEKHCPNGCAEHDTLAAAIAAYRP
                     PPPEAATTAGELAPTPTE"
     gene            complement(2021..2575)
                     /locus_tag="VG274_02045"
     CDS             complement(2021..2575)
                     /locus_tag="VG274_02045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE05459.1"
                     /translation="MRKTVYLAVAAALLVSPAFAMGGGGGGGYGGFNAMPSASFDDYT
                     TAKRLIKHEDYAKAIPHLEAALAKRPHDADILNYLGYTHRMVGMGEADTVRDTDFKTS
                     LAYYEQALAIDPNHKGVHEYLGELYLQMGDLNAAHHEMNQLMILCPDGCDERDTLNKA
                     LAAYVPPAAPAAMAPAAAAPAVTQ"
     gene            2675..3289
                     /locus_tag="VG274_02050"
     CDS             2675..3289
                     /locus_tag="VG274_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWE05460.1"
                     /translation="MDSETLTKTEARPARRRPESLAKLKKAARKLFVERGYHATRPQD
                     IAREAGLGHGTFYLHYDDKRACFLAFVEDARLELQEYVDARVAESDGSLEHTIAASLN
                     AIYDYSESHPGVLGAAMTDEAVIDAEGAQATPLLIRWGHDWAEHVKRSMAAGTATRAY
                     DPEIAGQMILGCIHQVGAEGYRAGRKRQELVDNLTRFLVRALKP"
     gene            3300..>3664
                     /locus_tag="VG274_02055"
     CDS             3300..>3664
                     /locus_tag="VG274_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05461.1"
                     /translation="MKLTCYSVFLFRDGWQQQQGGSWQSRDEAIKVVASECEADLEVF
                     EIRETKVLIGLGRRPYHNPRGGYKHRYSSDEKDEPNTLFIDAVETEEIRRLCRDGEAS
                     YREDQAEIKKRIAKSRKLE"
BASE COUNT          746 a         1061 c         1164 g          693 t
ORIGIN      
        1 cccgacatcg atttcgccta tgaggaaatc ccctgcaaga cgcacgcgat gggcgcaaag
       61 gggtgcggcg aagcaggcac ggtcggcgcg ctaccggtga ttatcagcgc catttgcgac
      121 gccatcggca tcgaacacct cgacatgccg gccacgcccg agcgcgtgtg gcgcgcgatc
      181 cgggacacaa aagcgcgggc gtaagcggcc ggggcttgcc ggatcggcac gacgcactat
      241 gtctccctag gccgggtttg gcgccgaggg gagactactg agatgaacac cgcgcattcc
      301 gggtcgctcg ttgggtttgc gctgcttgcg cttgcggcgc ccaacgccgt cttcgcagcg
      361 gataacattg cggctgagac ggttgtcgtc accgcaacgc gaaatctcga tgcgaacacg
      421 ctgggcacac tgtcgtccca gttcgtcgat tcccgcacgg cgctgtcacc gatcaggcag
      481 attgcgcgct ggcgtgcccc cgtctgtccg gcggttcagg gtttgccacc ggcgttcaac
      541 aaattcgtct ccgaccgcat caaggccatc gcggtcaagg tcggcgcgcc tgtcgatgcg
      601 tcctgcaagt cgaatatcga agtgatgttc acgcccgaaa cgcagacgat ggccaatgat
      661 gtcgcggaca agacgccctg gcttttcggc acgcgcctga cgacgagcgc gatgcacctg
      721 gcgaccgtca accatcccat ccaggcctgg tacgtgaccg caacgatcga tccgacaggt
      781 cagcagagaa cagacgaccc cgccgatccc gttggcgtca cgggctccaa ccgaaaaatc
      841 tccggaagcc ggctcggcgc gaacaagacc agcgttctcg aaaacgtact gatcattgca
      901 gatgcgggaa agctggcggg ccacgatatc ggcacggtgg cagactacat cgccttgctc
      961 gctctctctc agatgcagcc gtcagacgat tgtgcagcgt tgccgagtgt ggcggacgcg
     1021 atggcagcgt cctgcagcaa gaagcccgcg ccggactcga tcacggcggt cgatacggcg
     1081 tttctggagg ggctctatgc aactcagcct gatgtgctcg gctcaatcgc gcgtgcggac
     1141 atcgcgcggc gcacggcgca aggactgagc ggccaatagg ccgaagcggt caggcgtcgc
     1201 tcaacagcgt gttgcgcttg cggctgagca gcagaatcgc ggcatcgatc acaagcgccg
     1261 ccaaaaatgg cgacgccgcg atgccgacgc catcttcgtc ggcttccttc ttccaaagcg
     1321 cggcaaaggc aaagatgccg gccagaatca acaacgattt gcgcatgcgg aacttcctgg
     1381 ttcgattcta acaacccgaa cgcgctccgg gttgcggcgg ctcgggctac tcggtcggtg
     1441 ttggcgcgag ttcgccggct gttgtggctg cctccggtgg aggaggccgg tacgcggcga
     1501 tcgcggcggc gagcgtgtca tgctcggcgc atccgttcgg gcaatgcttt tcgagcgccg
     1561 cgagtttttc cctcgctgcg gcgacatcat ccatctcaag atagagctcg cccatatatt
     1621 cgaggaagtc cttgcggtcc ggatcgacat ccagcgcgcg gcggtaatac gtgttcgaca
     1681 agcgcaattc gccggcgtgg gaagtgccgg aatactgcct ggcgatcatt cggtgggcga
     1741 agccaagata tttcaggata tcggtgtcgt cgggaaattg ttgcagcgcc gtttcgagat
     1801 ggggcgtggc gtcgaggtat ttgtggtgat tgactagatc gacgccgatc gcgaattcgt
     1861 tccgggcgcg gatatcggcg ctcggactcg cgaaatcgat ggcggcggca ggtatacagg
     1921 ccgcaagcag aaccgccgcg gcgagggtaa tggccttgta cataggttgc tccttcgaag
     1981 ttcccgaggg aacaacgcgc aacccaaaca aaagtgcctt ctactgcgtc accgccggag
     2041 ctgcggctgc tggcgccatc gcggcgggcg cggccggcgg aacataggcc gcgagcgcct
     2101 tgttcagcgt gtcgcgttcg tcgcaaccat ccggacacag gatcatgagc tgattcatct
     2161 catgatgggc ggcgttcaga tcgcccattt gcagatagag ttcgccgaga tattcgtgca
     2221 cgcccttgtg attgggatcg attgcgagcg cctgctcgta ataggcgagc gacgttttga
     2281 aatcggtgtc gcgcacggta tcggcttcgc ccatgccgac catgcggtgc gtgtagccga
     2341 gataattcag gatatcggcg tcgtgcggac gtttggcgag cgctgcttca agatgcggaa
     2401 tggccttcgc atagtcctcg tgtttgatca ggcgtttcgc cgtcgtgtaa tcgtcgaagc
     2461 tcgcactcgg catcgcattg aagccgccat agccccctcc gcctccgccg cccatcgcga
     2521 aagccggcga gaccaacaat gctgctgcaa ctgcgagata aaccgttttg cgcatggcat
     2581 cctcccacgt tcgggaaacc atattagcgc gaacgactcg tgggttccat cgctatggtt
     2641 tgacttacag tgtacggaca gatgaaaatc gcgcatggac tcggaaacac tgacaaagac
     2701 tgaggcacgg cccgcgcggc gcagaccgga aagcctcgcg aagctcaaga aagcggcgcg
     2761 caagctgttc gtggagcgcg gctatcacgc gacacggccg caggatatcg cgcgcgaggc
     2821 gggtttgggt cacggcacgt tctatctgca ctatgacgac aaacgcgcgt gtttcctcgc
     2881 gttcgtggaa gatgcgcggc tggagctgca ggaatatgtc gacgcgcgtg tcgccgaaag
     2941 cgatggctcg cttgagcata cgatcgcggc gtcgttgaat gcgatctacg attattcgga
     3001 atcgcatccc ggcgtgttgg gtgccgcgat gacggacgaa gcggtgatcg atgccgaggg
     3061 cgcgcaggcg acgccgttgc tgatccgctg gggccacgac tgggcggagc atgtgaaacg
     3121 ctcgatggcc gccggcaccg cgacacgcgc gtatgaccct gagatcgcgg gccagatgat
     3181 cctgggctgc atccatcagg tgggcgccga aggctaccgc gccggccgca aacgccagga
     3241 gctggtcgac aatctcacgc gcttcctggt ccgcgcgctg aagccctagt catcgaccga
     3301 tgaaattgac ctgctacagt gtttttctct ttcgagatgg ctggcagcaa cagcagggtg
     3361 gcagttggca atcccgcgat gaagcgatca aggtagtggc gtctgagtgt gaagctgatc
     3421 tggaagtttt tgaaataaga gaaacgaaag tattgattgg attaggccgg cgcccctatc
     3481 acaatccaag aggcggttat aagcatcgat attcctcaga cgagaaagat gaaccaaata
     3541 cgttgttcat tgacgcggtg gaaacagaag aaattcgccg gttgtgcagg gatggcgaag
     3601 cttcgtaccg cgaagatcag gcagagatta agaagcgcat cgcaaaaagt cgtaagctcg
     3661 agca
//
LOCUS       DASZMC010000108         5547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2532048,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000108 DASZMC010000000
VERSION     DASZMC010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532048"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VG274_02060"
     CDS             complement(<1..242)
                     /locus_tag="VG274_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWE05462.1"
                     /translation="MMLFGAAIFFTEHSIGPIELAHALEERGFESLWAPEHSHIPVSR
                     KTAYPGGGELPKEYYDVMDPFVTLTVAAAATRSLKVA"
     gene            425..967
                     /locus_tag="VG274_02065"
     CDS             425..967
                     /locus_tag="VG274_02065"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HWE05463.1"
                     /translation="MKLPILALATAALLCGTSVVSAQESPNGGAPASDQNRNQRERPS
                     DQTKQPGAGQEAEPRNGSEEMQRGNAPSRDMERTEPQGGQMLRGGEMRERGGIRNLTV
                     EQKTNLRETVLRRGPRVTNINFRIGVGAVVPRSVRLVAVPQPIIAIYPEWSGDLYFDY
                     GDEIVVVAPDTLQIVGVLPV"
     gene            complement(1034..1258)
                     /locus_tag="VG274_02070"
     CDS             complement(1034..1258)
                     /locus_tag="VG274_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWE05464.1"
                     /translation="MAKSKSGAAVYRVTEIIGTSSTSWEDAAKSAVETASRSLRDLRV
                     AEVEKLDMKVENGKVVAYRARVSLSFKYEG"
     gene            1452..2996
                     /locus_tag="VG274_02075"
     CDS             1452..2996
                     /locus_tag="VG274_02075"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HWE05465.1"
                     /translation="MNGAESLVRTLVASGVEVCFANPGTSEMHFVAALDRVEGMRAVL
                     TLFEGVATGAADGYGRMAEKPAATLLHLGPGLANGLANLHNARRAATPIVNIVGDHAT
                     YHAQYDPPLASDIAGFARPVSAWIHSSMSAATVAEDGARAVQAARQAPGQIATLILPA
                     DTAWLEAERPAPPLPVQGPSRVSQQAIDDVAQALREGRRNALLLRGGALGRRGLEATG
                     RIAVKSGARILCDTFSPRITRGAGLVAAERLPYFAEQIVEFLRDFELLVLVGSKPPVS
                     FFAYPGKPSWCVPEGCRILHLAHPHEDAVGALEAVAEAIAAPRDPVGRAPLQRPDIPE
                     GKLNAYTAGQAIGHFLPEGAIISDEAATSGLGVASYTAGAPPHDLLSLTGGSIGLGVP
                     MATGAALACRDRKVVCLQGDGGAMYTLQALWTQAREKLDVTTVIFANRSYYILNIELM
                     RVGAENPGPKALSMLDLHNPELDWVSLARGMGVEASRVETNADFASAFADAMKSPGPR
                     LIEVVL"
     gene            3198..4634
                     /locus_tag="VG274_02080"
     CDS             3198..4634
                     /locus_tag="VG274_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWE05466.1"
                     /translation="MSDLLDDLTSFLGESHVLEGERAAEAALSTWSRLGAPLAVLKPS
                     STDQVSRVLSLCHAAGQPVVPWGGKTGLVEGANADGAFALSLERMNRIESIDQPGGTM
                     TVEAGCILQNVAEAADAHGLFFPLDLGARGSATIGGNISTNAGGNRVIRYGMTRDMVL
                     GLEAVLADGTVLASLNRLIKNNAGYDLKQLFIGTEGTLGIVTRAVLRLRAKPGSNAVA
                     LAAVDEFASLPRVLRQMERGLSGHLSAFEVMWEDFYRLVTTEPAKGRAPIAYGHPYYV
                     LIEQLGGDEASDAARFESALGEALEQGTIVDAVIAKSQAERNAIWSLRDDVGQCARNW
                     PIFTFDVSLPIGEMEAYVAQVRSELHSRWPGTATAMIFGHLGDGNLHLITGVGDGSPA
                     ARHAVEEIIYGALEGRSGSVSAEHGIGLEKRRYLAFSRSDAEIGLMRQIKQALDPRGI
                     LNPGKIFELQRLAVTNRQDAKTKSTGRQ"
     gene            4779..>5547
                     /locus_tag="VG274_02085"
     CDS             4779..>5547
                     /locus_tag="VG274_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWE05467.1"
                     /translation="MHRDLNRLANEVFDLLVIGGGITGAAIARDASQRGLSVALIERN
                     DFAHATSAHNSKLIHGGLRYLRNLELGLVRESLRERRIWQRIAPHLVRPLPFLLPVLD
                     PGFGARAKLSAGLSLYDLLSFDRGWLDDPDQRLPGHSWLDARAALACEPCLDSLPIAG
                     AFRYFDAQMYSPERLALECVLDAADNGAAPANHVEANTLLLRNNRIEGANVADSHAGA
                     TFDIRSKLTIVAAGPWADLFLARALGRTAHQLRRSKGI"
BASE COUNT          989 a         1807 c         1747 g         1004 t
ORIGIN      
        1 gcgaccttga gcgatctggt cgccgcggcc gcgaccgtca gcgtcacgaa gggatccatg
       61 acgtcgtaat attccttcgg cagctctccg ccgcccggat aggcggtctt tcgcgacacc
      121 ggaatgtggg agtgctcggg cgcccagagc gattcaaatc cgcgctcttc gagagcatgg
      181 gcgagttcga tgggaccgat cgagtgttcc gtaaagaaaa ttgccgcgcc aaacagcatc
      241 attgcacccg agattgattg cgaatgccat tttggaccgc acccaaaggg ttcggcaatc
      301 ccgctgtctg caggcaaccg gcaacgcaat gcggcaattc tgcggcgggg aaccgcgcca
      361 gcagggggac gttttttcaa tgaccgataa cggtcgatgc tgcgggctta tcaggagata
      421 tctcatgaaa ctgccaatct tggccctggc caccgcagcg cttctgtgtg gcacgtctgt
      481 tgtgtcggcg caggaaagcc ctaacggcgg tgcacctgcg agcgaccaaa accgcaatca
      541 gcgcgagcgg ccaagcgacc agacgaagca acctggcgcc ggtcaggagg ccgagccgcg
      601 caacggttcc gaagaaatgc agcgcggcaa cgcgccgtcg cgcgacatgg agcgcaccga
      661 gccccagggc ggccagatgt tgcggggggg agaaatgcgc gagcgtggtg gcattcgcaa
      721 cttgacggtc gaacagaaga cgaatttgcg cgagaccgtc ctgcgccgcg gcccgcgggt
      781 gacgaacatc aatttccgca tcggcgtggg ggcggtcgtt ccgcgctccg tccggctggt
      841 tgcggtgcct cagccgatca tcgccatcta tccggaatgg agcggcgacc tgtacttcga
      901 ctatggcgac gaaatcgtcg tggtcgcccc cgacacgctg cagatcgtcg gcgttcttcc
      961 ggtctgacga actcgatgga agggcggcgc ccgccgcctt tcccttcgcc tccgcccagg
     1021 cttcgtacct gaactagcct tcgtatttga aggagagcga aacgcgggca cgataggcga
     1081 cgactttgcc gttctcgacc ttcatgtcca gcttttcgac ttcggcgacg cgcagatcgc
     1141 gcaatgaccg cgatgcagtt tcaacggccg atttcgccgc gtcctcccac gatgtcgagc
     1201 tggttccgat gatctcggtg acacgataga cggcggcgcc ggatttggac tttgccatgt
     1261 ttcctctcct gccggctctc tgaagcgacg accgctgtgc tccggcggct ggcaggatat
     1321 ccccgctttc cgccaaaccc aacccgtgtg gccctattcc gccagggaac gcggttttcg
     1381 gcttccctta tgccggccgc atcctaagat cgcactacgt gccccaggcc cggggcggcg
     1441 acgggtgaaa catgaacggc gcagaaagcc tggtccgcac gctggtcgct tctggcgtcg
     1501 aagtttgctt cgccaatcca ggcacttccg agatgcattt cgtggcggcg ctcgaccggg
     1561 tggaaggaat gcgcgcggtg ctgacgcttt tcgaaggcgt tgccacgggg gcagccgacg
     1621 gctacgggcg catggccgaa aaaccggccg ccacgttgct gcatctgggc ccgggtctgg
     1681 cgaacggcct cgccaatctc cacaacgcgc gccgcgccgc cacgcccatc gtcaacatcg
     1741 tgggcgatca cgccacctat cacgcccaat acgatccgcc gctcgcgtcc gatatcgcgg
     1801 gatttgcgcg tcccgtttcg gcctggatcc attccagcat gagcgccgcg acagtcgccg
     1861 aagacggcgc gcgggctgtg caggcggcgc ggcaggctcc gggacagatc gccacgctca
     1921 ttctcccggc cgacacagcc tggctggaag cggagcgtcc agctccgcct ctaccggttc
     1981 agggaccgag ccgcgtttcc caacaagcga tcgacgacgt cgcgcaagcg cttcgcgaag
     2041 ggcggaggaa cgccctgctg ctgcgcggcg gcgcgcttgg acgtcgcgga ctcgaagcca
     2101 ctggccgcat cgcggtgaaa tccggcgcgc gaatactgtg cgacacgttc tcgccccgga
     2161 tcacgcgcgg ggcgggactc gtggcggccg agcgcctgcc ctatttcgcc gagcagatcg
     2221 tggagttcct gcgggatttc gaattgctcg ttcttgtcgg ttcgaagccg ccggtttcat
     2281 tcttcgccta tcccggcaag ccgagctggt gcgttccgga aggatgccgg atcctgcatc
     2341 tcgcgcaccc gcatgaagat gcggtcggcg cgctcgaagc tgtcgctgag gcgatcgcag
     2401 cgccacgaga tcccgttggc cgcgctcccc tccaaagacc ggacattccg gagggcaaac
     2461 tgaacgccta cacggccgga caggcgatcg gtcatttcct tcccgaaggc gcgatcatct
     2521 ccgacgaagc cgcgacgtcg ggactgggcg tcgcttccta cacggcgggt gctccgccgc
     2581 acgatctgct ctcgctgacc ggcggttcga tcgggctcgg cgtgccgatg gccaccggcg
     2641 ccgccctcgc ctgtcgcgac cgcaaggtgg tctgcctgca gggcgatggc ggtgcgatgt
     2701 acacgcttca ggcgttgtgg acgcaggcgc gcgaaaagct ggacgtgacc acggtgatct
     2761 tcgccaaccg ctcctactac atcctcaata tcgagctgat gcgcgtcggc gcggagaacc
     2821 cgggccccaa ggcgctttcg atgctcgacc tccataaccc cgagctcgac tgggtaagcc
     2881 tggcccgcgg aatgggcgtg gaagcatcgc gtgtggaaac caacgccgat ttcgcatccg
     2941 ccttcgccga cgcgatgaag tcgcccggac ctcggctgat cgaagtcgtg ctctagtacc
     3001 tggaatcaat gacgagtgac acaaagacag atttaacgca gagggcgcag aggcagcgca
     3061 gtgggcgcgg aaaagagttg gcgcgccgca ggcgcgcggt tcttgcccgg cgtcctccgc
     3121 gcatccactg cgtactctgc gttaaatctg ctggccgtga gtcagcaaca gtcaaaagat
     3181 gatactaggc ccttctgatg agcgatctgc ttgacgatct gacatcattt ctcggcgagt
     3241 ctcacgttct cgaaggcgaa cgtgcagcgg aagcggcgct cagtacctgg agccggctcg
     3301 gcgcgccgct cgcggttctg aaaccttctt cgaccgacca ggtttcgcgc gttctgagcc
     3361 tgtgtcacgc agccgggcag cctgttgtgc catggggcgg caagaccggt cttgtcgagg
     3421 gcgcaaatgc ggacggcgcg ttcgcgcttt ccctcgagcg catgaacaga atcgaatcga
     3481 tcgatcagcc cggcggcacg atgacggtcg aggccggttg catcctgcag aatgtggccg
     3541 aagcggcaga tgcgcacgga ctgttctttc ccctcgatct tggcgcccgg ggctccgcca
     3601 cgatcggcgg aaacatctcc accaacgccg gcggcaaccg cgtcatccgc tacggcatga
     3661 cgcgcgacat ggtgctgggc ctcgaagcgg ttctcgccga cggcaccgtg ctcgcttcgc
     3721 tcaaccgtct catcaagaac aacgccggct acgacctgaa gcagctcttc atcggcaccg
     3781 aaggcacgct cgggatcgtc acccgcgccg ttctgcgcct gcgggcaaaa cccggaagca
     3841 atgccgtagc gctcgcggcc gtggacgaat ttgcgtcgct tccgcgcgtt ttgcgacaga
     3901 tggaacgcgg actctccggt cacctctcgg ccttcgaagt gatgtgggag gatttctacc
     3961 gcctggtgac cacggaacct gcgaaggggc gcgccccgat tgcctatggc catccctatt
     4021 acgtgctcat cgagcaactg ggcggcgacg aggcgtccga cgcggcgcgt ttcgagagcg
     4081 cgctcggcga agctctggaa cagggcacga ttgtcgacgc ggtgatcgcc aagtcgcagg
     4141 cggaacgcaa cgcgatctgg tcgctgcgcg acgatgtcgg gcaatgcgcc cgcaactggc
     4201 ccatttttac attcgacgta agcctgccta tcggcgagat ggaagcttat gtcgcccagg
     4261 ttcgatccga attgcattcg cgatggcccg gcacggcgac tgccatgatt ttcggccacc
     4321 tcggcgacgg caatctgcat ctcataacgg gggtaggtga tggcagccct gccgcgcgcc
     4381 acgccgtgga ggagatcatt tacggcgcgc tcgaaggacg ctccggttcg gtctccgccg
     4441 agcacggcat cggactcgag aagcgccgct atctcgcctt ctcgcgctcg gacgccgaaa
     4501 tcgggctgat gcgccagatc aaacaagcgc tcgacccgcg cggcatactc aatcccggaa
     4561 aaatcttcga actgcagcgg ctcgccgtga cgaaccgcca agatgccaag acgaaatcca
     4621 cagggagaca gtgagacggt gagcaaagaa ggcgcgccta aggcgcgcaa gaaattctca
     4681 ctgcctcatt gcctccttgt gaatcttcgc cgaggtacgg acgccttggc gacttggcgt
     4741 cttggcggtt cgatccggcg cgacgggggc accgattaat gcaccgggat ttgaaccggc
     4801 ttgcgaacga ggtgttcgat cttctcgtca tcggcggagg aattacgggc gccgcaatcg
     4861 cgcgggatgc gagccagcga ggactctcgg tcgcgctgat cgagcgcaac gattttgcgc
     4921 atgcaaccag cgcgcataat tccaagctga ttcacggcgg cctgcgctat ctgcgcaacc
     4981 tcgagctcgg tctggtgcgt gagtcgctgc gggaacgccg tatctggcaa cgcatcgccc
     5041 cgcacctcgt gcggccattg ccgtttctgc tgcctgttct cgaccctggg tttggcgcgc
     5101 gcgccaagtt gagcgcgggc ctctcgttgt acgatctcct gtccttcgac cgcggctggc
     5161 tcgacgatcc cgatcagcgg ctgcccggcc attcctggct ggacgcgcgg gccgccctcg
     5221 cctgcgagcc ctgtctcgat tcactgccca tcgcaggcgc gttccggtat ttcgacgcgc
     5281 agatgtactc gccggagcgg ctcgcgctcg aatgcgtact cgacgccgcc gacaacggcg
     5341 ctgcgcccgc caaccatgtc gaagccaaca cgctgctctt gcgcaataac cggatcgaag
     5401 gggcaaacgt cgccgacagc cacgcgggcg cgacattcga cattcgctca aaactgacga
     5461 tcgttgcggc cggaccctgg gcggatctgt ttcttgcacg cgccttgggc cgcaccgcgc
     5521 atcagctgcg gcgctccaag ggcattc
//
LOCUS       DASZMC010000109         4941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_867844,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000109 DASZMC010000000
VERSION     DASZMC010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867844"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VG274_02090"
     CDS             complement(<1..596)
                     /locus_tag="VG274_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05468.1"
                     /translation="MIAAALPFVLFGFVAPALSQTADELIAKNLAARGGADRLAAIHS
                     VVSRGELRFPGDFKLAFKETKSRTGGSANAARIEATIEGLTLIQAYDGANAWRVNPFE
                     GRKDAERMGADETRELADDESIDGALLAARTRGSQVDYLGREDIDGTNAYKLRVTQPD
                     GTVFTYYLDPDAFLEIKVLERRTIRGAVKETETDLADY"
     gene            785..1534
                     /locus_tag="VG274_02095"
     CDS             785..1534
                     /locus_tag="VG274_02095"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002343.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HWE05469.1"
                     /translation="MDTPVTDQRTARVPQFFLTPGGPCPYLPGRTERKVFARLNGSLA
                     QPLNEALTHSGFRRSQMIAYRPACDGCSACVSVRIVTEKFAPSRSQKRLSRRNGDLVR
                     SEAAPQATREQFALLRTYLDSRHAGGGMSDMGLFDYVAMVEETPVNTHIVEYHDAPAN
                     DRPSRLVACALTDVLRDGLSMVYSFFHPGEDARSLGTYMILDHVSAALARGLPYVYLG
                     YWIRGSAKMNYKARFRPLEALGADGWRPLNT"
     gene            complement(1666..3387)
                     /gene="metG"
                     /locus_tag="VG274_02100"
     CDS             complement(1666..3387)
                     /gene="metG"
                     /locus_tag="VG274_02100"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWE05470.1"
                     /translation="MDSILVTSALPYINGVKHLGNLVGSMLPADVFARFQRARGRRTL
                     YICATDEHGTPAELAALEAGQDVATYCTEQHRIQEELGQRFGLSWDFFGRSSHRANHE
                     LTQHFARELWRRGHLDIRVTRQVYSIADGRFLPDRYVIGTCPHCGYERARGDQCENCT
                     RVLDPADLIHPRSALSGATDIEIRDSAHLFLRQSQFVDALRAWIDSHRDDWPSLVTSI
                     AYKWLDEGLHDRGITRDLEWGVPVPDDIGDGKLKGKVFYVWFDAPIAYISATREWSEA
                     NGAGEEWRNWWIGDPARAVTYYEFMGKDNVPFHTVGFPVTILGSGESWKLADRIKGFN
                     YINYCGGKFSTSQRRGVFMDSALEILPADVWRYYLMANAPESSDSNFTWEHFASVVNK
                     DLADVLGNFVNRVMKFCGARFDSRVPGEGRWGAEETALAEALDPRVRAYTDYLEACEF
                     RKALAELRAIWVAGNEYLTRAAPWTSISTDRDRAAVGVRAGLNLVHLFGHLAWPVIPD
                     VARRIHESIMPAPEIIPWPEEPMEEFLDQLTAGMRVRAPDMLFAKITDDQIAEWQRRF
                     AGEQNSG"
     gene            3511..3586
                     /locus_tag="VG274_02105"
     tRNA            3511..3586
                     /locus_tag="VG274_02105"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3544..3546,aa:Thr,seq:tgt)
     gene            3838..4701
                     /locus_tag="VG274_02110"
     CDS             3838..4701
                     /locus_tag="VG274_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05471.1"
                     /translation="MSALLLAGVSLLAAAPSCAEETIHHPAAHARPVARPAPFRGGVR
                     FGARFGRPVFHPLHAAIFAHRSFARFTPAERVAWTHGRWSHRWWHGRFGWWWNAGGVW
                     FWYDAPVYPYPTVVSDNYYEEPDYDQAAPAWYYCYNPPGYYPYVPSCRGPWTPVPAAT
                     PEGYGNDQGGPDQRPPPGASHGMNNEQGPPPGEGPGDGQGPPPGYEQGPPGYDQGPPS
                     GYNQGPPGNEQGPPPGYNQGPPGGDQGPPSGYDQGPPPGYDQGSPPGDDQGPPPNDDQ
                     GPPNNQQGTHE"
     gene            4694..>4941
                     /locus_tag="VG274_02115"
     CDS             4694..>4941
                     /locus_tag="VG274_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05472.1"
                     /translation="MSRIRTIFLSTALAGLVSGCVTPPMGPSIPVMPGPNKSLAAFQQ
                     DEGVCEDYASHRVAGQAEAANNTALGEGLLGTALGAGLG"
BASE COUNT          901 a         1589 c         1539 g          912 t
ORIGIN      
        1 tcgtaatcgg caaggtcggt ttcggtctcc ttcactgcgc cgcggatcgt gcgccgctcg
       61 agtaccttga tttccaggaa cgcgtcggga tcgagataat aggtgaagac tgtgccgtcc
      121 ggctgggtca cgcgaagctt gtaggcattg gtgccgtcga tgtcctcgcg gccaagatag
      181 tccacctggg aacccctcgt gcgcgcggcg agcagcgcac cgtcgatcga ttcatcgtcg
      241 gcaagttcgc gcgtctcgtc ggctcccatg cgctccgcgt ctttgcggcc ttcgaaggga
      301 ttgacgcgcc aggcattcgc gccgtcatag gcctgaatca acgtgagccc ttcgatggtg
      361 gcttcgatgc gcgcggcgtt tgcgctgccg ccagtccgcg atttggtctc cttgaaggcg
      421 agcttgaaat cgccggggaa tcgcaactcg ccacggctga cgacggagtg gattgccgca
      481 agcctgtccg caccgcctcg ggcggcgagg tttttggcaa tcagttcatc cgctgtttga
      541 gaaagtgccg gagcgacgaa gccaaacagc acgaacggca acgctgccgc gatcattcga
      601 caatgcaagg aggtttcctc ggagcgcgcc gccaaagccc catgacttgg ggggccgctc
      661 cgcgaaactc gcccatgcgc cggttccggt caaggcgacg ggcgcgaaga agccattcgt
      721 tttcgggtgg tgggccttgc ggcgaacgcc gaacagcact catagtcgcg ccacacgggg
      781 atatgtggac acgccggtga ccgaccagcg aacagcgcgc gtaccgcaat tctttctcac
      841 gccgggcggc ccctgccctt atctcccggg ccggacggag cgaaaggtgt tcgcccgcct
      901 caacggctcc ctggcgcaac cgctcaatga agcgctcacc cattcgggat ttcgccgcag
      961 ccagatgatc gcctaccggc cggcctgcga cggctgttcg gcgtgcgttt ccgtgcgcat
     1021 cgtgacggaa aaattcgccc cctcccgaag ccaaaagagg ttgtcccggc gaaacggcga
     1081 tctggtgcgc agcgaagcag cgccccaggc gacacgtgaa caatttgcgc ttctgcgcac
     1141 ctatctcgat tcgcgccatg cggggggcgg catgtcggat atgggactgt tcgactatgt
     1201 cgcgatggtg gaagaaacgc cggtcaatac gcacatcgtc gaatatcacg atgcgccggc
     1261 aaacgacagg ccgtcgcggc tggtcgcctg tgcccttacc gacgtgctgc gtgacgggtt
     1321 gtcgatggtc tacagttttt ttcaccccgg cgaggacgcg cgcagcctcg gcacttacat
     1381 gattctcgac catgtttccg cagcgctcgc gcgcggcctg ccttacgtct atctcggcta
     1441 ctggatccgc ggcagcgcga agatgaacta caaggcgcgc ttccggcctc tggaagcact
     1501 cggcgccgac ggctggcggc ctctgaacac ctgaccgggc tcgatcccaa aaaaattgtc
     1561 atgcccggct cgaccgggcc acccagaagt caacgcacgt cgcttccatc tgggtggccc
     1621 gcttgtgcgg gccatgacaa ttttgggggg tgcacttctc acatttcagc cgctattttg
     1681 ttcacccgca aagcggcgct gccactccgc gatctgatca tcggtgatct tcgcaaacag
     1741 catgtccggc gcccgcacgc gcattcccgc ggtcagctgg tcgagaaatt cctccatcgg
     1801 ctcttcgggc caggggatga tttcgggcgc cggcatgatc gactcgtgaa ttctgcgtgc
     1861 gacgtcggga atcacgggcc acgccagatg cccgaacaga tgcacgagat tgagaccggc
     1921 acgcacgccg acagccgccc ggtcccgatc ggtcgaaatc gaagtccacg gtgcggcgcg
     1981 ggtgagatat tcgttgccgg ccacccagat ggcccgcagc tcggcaagcg ccttgcgaaa
     2041 ttcgcaggcc tcgagatagt ccgtatacgc acgcacccgc gggtcgagcg cctccgcgag
     2101 cgcagtttcc tcggcgcccc accggccttc gcccggcacg cgcgagtcga accgcgcgcc
     2161 gcagaatttc atcacccggt tgacgaaatt gcccaatacg tcggcgaggt ccttgttgac
     2221 gacggaggcg aagtgttccc aggtgaagtt cgaatccgag ctctccggcg cgttcgccat
     2281 caggtagtag cgccacacat cggcaggcag aatctccagc gcggagtcca tgaacacgcc
     2341 gcgcctctgg ctggtcgaga attttccgcc gcaatagttg atgtagttga agcccttgat
     2401 gcggtccgcg agcttccagg attcccccga accgagaatg gtgacgggaa atcccacggt
     2461 gtggaacggc acattgtcct tgcccatgaa ttcgtaatag gtgacggcgc gcgccggatc
     2521 gccgatccac cagttgcgcc attcctcacc ggcgccgttc gcctccgacc attcacgcgt
     2581 tgccgaaatg taggcgatgg gcgcatcgaa ccagacgtag aaaacctttc ccttcagctt
     2641 tccgtcgccg atatcgtcgg gcacggggac gccccattcg aggtcgcggg tgatgccacg
     2701 gtcgtggagg ccttcatcca gccatttgta ggcaatcgaa gtgaccaatg acggccagtc
     2761 gtcacggtgg ctgtcgatcc aggcgcgcaa tgcgtctacg aattgcgatt gcctgaggaa
     2821 gagatgggcg ctgtcgcgga tttcaatatc ggtggcgccg gacaaggccg accgcggatg
     2881 gatgaggtcc gcgggatcga gaacgcgcgt gcagttctcg cactgatctc cccgcgcgcg
     2941 ctcgtaaccg caatgggggc aggtgccgat gacgtaacgg tcgggcagga agcggccgtc
     3001 ggctatggag tagacctgcc tggtaacacg aatgtcgagg tggcctcgcc gccatagctc
     3061 gcgcgcgaaa tgctgggtca gctcatgatt ggcccggtgc gaggatcggc cgaaaaagtc
     3121 ccacgacagg ccgaagcgct ggcccagctc ttcctgaatc cgatgctgtt ccgtgcaata
     3181 ggtggcgaca tcctgacccg cttccagggc ggcgagttcc gcgggcgtgc catgttcgtc
     3241 cgtcgcgcag atgtagagag tccgccggcc gcgcgcgcgc tgaaatcgtg cgaacacatc
     3301 cgccggcagc atggagccga cgagattgcc gagatgcttg acgccgttga tgtatggcag
     3361 cgcgctggta acgagaatcg aatccatgaa actctgcaat caattcgtgg gcatttggcc
     3421 caacataccg atgcgcgcga ttgcgtccac cccgcgcgcc gcccgaaatc gcgttgcacg
     3481 ccgccgccgc gcagcgtatg tcgcgcctgg gccggcttag ctcagctggt agagcacgtg
     3541 atttgtaatc acggggtcgc aggttcgatc cctgcagccg gcaccacaat tcagccgcgg
     3601 ccgacaaagt tcgcggaggt ctctccatcg ctcggaagtg ctgaatccgt catttgcgta
     3661 tcgctgccgc ggatattgag cgcctctcag acccgcaggc acgattcgag atgccatcgc
     3721 ctcgaaatcg ttcaacctta tcaagcgcgt gccgttcatc ttcggttcag cttgaatgga
     3781 ttagtcatcc cgcgaaactc gggtcaaacc atcatgcaat atatgcatcg aatctcgttg
     3841 agtgcgctgt tgctcgcggg cgttagcttg ctcgcggctg cgccgtcttg cgctgaggaa
     3901 acgattcatc atcctgccgc gcatgcgcgt cccgtagcgc ggcccgctcc gttccgcggc
     3961 ggcgtgcgat tcggcgcgcg cttcgggcgg ccagtttttc atccattgca cgctgcaatc
     4021 ttcgcgcatc gcagcttcgc gcgcttcacg cccgctgagc gtgtggcatg gacgcacggc
     4081 cgctggtccc atcgatggtg gcatggacgg tttggctggt ggtggaacgc gggcggcgtg
     4141 tggttctggt acgacgcccc cgtttatccg tacccgaccg tggtctccga taactattac
     4201 gaggagcctg actacgacca ggcggctccc gcctggtatt attgctacaa cccgccggga
     4261 tattaccctt atgtgcccag ttgccgggga ccgtggacgc cggtgcccgc ggcgacgccc
     4321 gaaggttacg gcaacgatca aggcggcccc gaccagaggc ccccgccggg cgcttcacat
     4381 ggcatgaaca atgaacaggg tccgccgcct ggtgaaggcc cgggcgacgg gcaaggcccg
     4441 cctcctgggt acgagcaagg cccgcccggc tacgaccagg gaccgccttc cggatataac
     4501 cagggcccgc ccggcaacga gcagggaccg cctcccggat ataaccaggg tccgcccggc
     4561 ggtgaccagg gccctccatc cgggtatgac cagggaccac cgcccggcta cgatcaggga
     4621 tctccacccg gcgacgacca aggcccgccc cccaacgacg atcagggacc acccaacaac
     4681 cagcagggaa cgcatgagta gaattcgaac gatttttctg tcgactgcgc tcgccggcct
     4741 cgtcagcggc tgcgtgacgc cgccgatggg cccctcgatt ccggtcatgc cggggccgaa
     4801 caaatcgctg gccgcgttcc agcaggatga aggcgtctgc gaagactatg cgagccatag
     4861 ggtggcaggt caggcggaag ccgcgaacaa caccgctctg ggcgagggtc tcctcggcac
     4921 agcgctgggc gccggactcg g
//
LOCUS       DASZMC010000110         5610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4720433,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000110 DASZMC010000000
VERSION     DASZMC010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4720433"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..890
                     /gene="rpe"
                     /locus_tag="VG274_02120"
     CDS             231..890
                     /gene="rpe"
                     /locus_tag="VG274_02120"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWE05473.1"
                     /translation="MTARVRIAPSILSADFSRLGAEVEAVERAGADVIHVDVMDGHFV
                     PNLTIGPVVVKALRPHAKKPLDVHLMIAPADPYIEAFAEAGADGLTVHPEAGPHIHRT
                     LQLIRAYGKKPGVVLNPGTPIDALDYLLDLVDLVLIMSVNPGFGGQEFISSQLRKIEA
                     AAERIARSGREIALEVDGGITPQTAKLAIGAGANVLVAGTAVFHGGPDSYAANIAALR
                     G"
     gene            841..2634
                     /locus_tag="VG274_02125"
     CDS             841..2634
                     /locus_tag="VG274_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HWE05474.1"
                     /translation="MADLTLTLRTSPRSAASGVAREAARRRRGPTLAMIPDLWRAVIR
                     RALTPLRIAWRRNWIYRRLLKGRMPDRIRHHPFDSLPRRLEDADMLLKGRFRFAGEMV
                     DAKEVSIFDCPAPSRNWASALHGFGWLASLSAAGGDAARNLAIQLVSEWMARNARYAE
                     PAWLPEVTARRLVQVFAHGKFMLANSDVLWRSKLFVSLRQQTAQLGRIAGEAPPGLPR
                     LEATAAYVLSCACLDDNPRRLESGLARLDREIATQILPDGGHVTRSPEELLLAYRQIV
                     MSVDALAAVGVAVPTGLRGARDRLAPMLRFFRHGDGALALFNGGGEGDAGMIAALLAR
                     DEVRGQPFLHAPHSGYQRLAAARSVAILDCGGLPPAQFSQHAHAGCLAFEFSASAQRL
                     VVNCGSDRESSPRWDGALRATAAHSTVTLADTSMAPVLAPGLARDLLGPRLLGGPVRI
                     ETERHETPHGWRVEASHDFYLQEFGIRHQREMMLSKQGTKLSGCDRLFPEPARRRRGA
                     VTFAARFHIHPDVRVSLSHRGDILLKLPGGEGWRFRHGGMISVEESVYVGQGSVRRAE
                     QLVLSGFVKDDPVETAWTFEQIGADASTGSA"
     gene            2729..3523
                     /locus_tag="VG274_02130"
     CDS             2729..3523
                     /locus_tag="VG274_02130"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HWE05475.1"
                     /translation="MVQFTLPKGSQPQKGKSWPAPVTANGKKPKRTKDFKIYRYDPDE
                     GGNPRIDTYRVDLESCGPMVLDALIKIKNEIDPTLTFRRSCREGVCGSCAMNIDGGNT
                     LACTKAISDISGSATIYPLPHLHVVKDLVPDMTNFYAQYASIQPYLQTRTAEPEREWR
                     QSEEDRAKLDGYYECILCACCSTSCPSYWWNSERYLGPAALLQAWRWLADSRDEARGE
                     RLDKLEDSFRLYRCHTIMNCAQTCPKGLNPARAIGEIKQMLVKRSI"
     gene            complement(3613..4467)
                     /locus_tag="VG274_02135"
     CDS             complement(3613..4467)
                     /locus_tag="VG274_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWE05476.1"
                     /translation="MIGAVVYASGWTLDDIPWDSFDPARVEGGLLAAVKSAALVELNA
                     PDYVSYLQRVFRDAGAKTVSDIERWGREEVQHGLALGRWAEIADPSFDFESAFARFRA
                     GYRPPHFTADDPDSVRGSRRGEMIARCVVESGTSSFYSAIRDAADEPVLKEVAGRIAA
                     DEFRHYKLFLDTLHAQDEPELPWWRRILVAATRVNESSDDELAYAYYCANVPASAEAN
                     IPYQRRRFARAYQARAMGLYRRHHIGKLVQMVAKSVGANPQGRLTRAASAVLWSILRL
                     RAGLAPAG"
     gene            complement(4535..4936)
                     /locus_tag="VG274_02140"
     CDS             complement(4535..4936)
                     /locus_tag="VG274_02140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF001851.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWE05477.1"
                     /translation="MTDKIAFDIVSPERLLLSDEADMVTVPGSEGDFGVLAGHMPLIS
                     TLRPGVVEIRGGGECGDVRFFVLGGFAEANPRKLTVLAEEAVPMTAIDAATLDQRIKN
                     TEEDLVLAKNEGDRARVAEQLDHLRQLRSAL"
     gene            complement(4938..>5610)
                     /locus_tag="VG274_02145"
     CDS             complement(4938..>5610)
                     /locus_tag="VG274_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWE05478.1"
                     /translation="DNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTLQERITTT
                     TKGSITSVQAIYVPADDLTDPAPATSFAHLDATTVLSRAIFEKAIFPAVDPLDSTSRL
                     LDPRVVGEEHYNVARQVQQILQRYKALQDIIAILGMDELSEEDKLTVARARKIERFLS
                     QPFYAATVFTGMSGITVKLEDTIRSFKAVVNGEFDHLPEAAFYMVGTIEDAVAKAQKL
                     AAEAA"
BASE COUNT          995 a         1771 c         1803 g         1041 t
ORIGIN      
        1 cgccgcgcgc gtcttgtcga aatgccagtg acgatggcgc acacgattgc cgcggccgag
       61 cttccgggtg tccatcgcga tccgtgggat cggatcctgg tggcgcaagc ccgaagcgct
      121 gaccatagtc accaccgacc ccgtgctcga atcctatgcc gtgaaaacgc tgtgttaatg
      181 cgtttcctgc cttgcgaggc gcgagcgaat ccgtcactac gtcacgtctg atgaccgcaa
      241 gggtccggat cgcgccttcg atactctccg cggatttctc gcgcctcggc gccgaagtgg
      301 aggcggtcga gcgcgccggc gccgacgtca tccatgtcga cgtcatggac gggcatttcg
      361 tacccaacct caccatcggc cccgtcgtgg tgaaggcgct gcgaccgcac gcaaagaagc
      421 cgctcgacgt gcatctgatg attgcgcccg ccgatcccta catcgaagcc ttcgcggaag
      481 caggcgccga cgggttgacg gtgcatcccg aggccgggcc acacatccac cgcacgctgc
      541 agctcatccg cgcgtatggc aaaaagccgg gcgtggtgct caatcccggc acgccgatcg
      601 acgctctcga ttaccttctc gatctggtcg atctcgttct gatcatgtcc gtcaatcccg
      661 gtttcggcgg ccaggaattt atttccagcc agctccgcaa gatcgaagcg gcggcggaac
      721 gcatcgcgcg cagcggacgc gagatcgcac tcgaggttga tggcggcatc accccgcaaa
      781 cagcgaagct tgcgatcggc gcgggcgcga acgtgctcgt cgccggaacg gcagtgtttc
      841 atggcggacc tgactcttac gctgcgaaca tcgccgcgct ccgcggctag cggcgtggcc
      901 cgcgaggcgg ccaggcggcg gcgcggtccg accctggcga tgatcccgga tttgtggcgg
      961 gcggtaattc ggcgtgcgct cacgccgctc aggatcgcgt ggcggcgaaa ctggatctat
     1021 cgccgcctcc tgaagggcag gatgcccgac cgcatccgcc atcatccttt cgactctctg
     1081 ccgcggcgcc tggaagacgc cgacatgctg ctgaaggggc gcttccgctt tgcgggagaa
     1141 atggtggacg ccaaagaggt ttcgatcttc gactgccccg cgccgagccg caattgggcc
     1201 tccgccttgc acgggtttgg ctggcttgcg tccctgtccg cagcgggcgg cgatgctgcg
     1261 cgcaaccttg cgattcaact cgtgtcggaa tggatggcgc gcaatgcgcg ctatgcagag
     1321 ccggcctggc tgcccgaagt aacggcgcgg cgtctggtac aggtgttcgc gcatgggaag
     1381 ttcatgctcg ccaattccga cgttctctgg cgctcgaagc tattcgtgtc gctccgccag
     1441 cagacggcgc agcttggccg catcgccggc gaggcgcccc ccggcctgcc gcggctcgaa
     1501 gcaaccgccg cctatgtact gtcgtgcgcc tgcctcgacg acaatccgcg gcggctcgaa
     1561 tccggactcg cgcgcctcga ccgggagatt gcaacgcaaa tccttccgga cggcggacac
     1621 gtgacacggt cgccggagga gcttctgctt gcctatcgcc agatcgtcat gagcgtcgat
     1681 gccctcgcgg cagtgggtgt ggcggtgcct accggtttgc gcggcgcccg cgaccgcctc
     1741 gctccgatgc tgcgtttctt tcgccatggc gatggggcgc tggcgctgtt caatggcggc
     1801 ggcgagggtg atgcgggcat gatcgcggcg ctgctcgcgc gcgacgaagt ccgcggccag
     1861 ccgttcctgc atgcgccgca ttccggctac cagcgcctcg ccgccgcgcg gtccgttgcg
     1921 atcctggatt gcgggggcct gccgccggcg caattttccc agcacgccca tgccggatgt
     1981 ctcgcgtttg aattcagcgc gagcgcacaa aggctggtgg tcaattgcgg ctccgatcgc
     2041 gagagctctc cgcgctggga tggggcgctg cgcgcgaccg ctgcgcattc caccgtgacg
     2101 cttgccgaca catcgatggc gcccgtgctg gcgccagggc ttgctcgcga tctgctcgga
     2161 ccgagacttc tcggtgggcc ggtgcggatc gaaacggagc ggcacgaaac tccgcatggc
     2221 tggcgcgtgg aagcgagcca cgatttctat ctgcaggaat tcggaatccg gcatcagcgc
     2281 gagatgatgc tgtcgaaaca gggcacgaag ctctccggct gcgatcggct ttttcccgaa
     2341 ccggcacggc gcaggcgcgg cgcggtgacg ttcgcggcgc gttttcatat ccatccggac
     2401 gtgcgggttt cgctgtcgca ccgcggtgac atccttctca agttgccggg tggcgaaggc
     2461 tggcgcttcc gccatggcgg catgatctcg gtggaagaaa gcgtctatgt ggggcaggga
     2521 agcgtgcgcc gcgccgagca gctcgttctt tcaggatttg tcaaagacga tcctgtcgag
     2581 accgcctgga ctttcgaaca gatcggcgcg gacgcttcga caggctcggc atgagcgaac
     2641 aaggccctca tggtgagcct gtcgaagcat gaatttgtca cgcgcgcacc ggcttgctat
     2701 ccagaattgc gcgccattcg aggtctagat ggtccagttc acgctaccaa agggaagcca
     2761 gccccaaaag gggaaatcgt ggcccgcacc cgtcaccgca aatggcaaaa aacccaagcg
     2821 gacgaaagac ttcaagatct atcgttacga tccggatgaa ggtggaaatc cgcgcatcga
     2881 cacctatcgc gtcgatctgg agagctgcgg gccgatggtc ctggacgcgc ttatcaagat
     2941 caagaatgag atcgacccga cactgacctt ccggcgttcg tgtcgcgaag gggtctgcgg
     3001 ttcctgtgcg atgaacatcg atggcggcaa cacgctcgcc tgcaccaagg ccatctccga
     3061 catttcgggg tcggcgacca tctatccatt gccccacttg catgtggtca aggatctggt
     3121 gcccgacatg acgaatttct acgcgcaata tgcctcgatc cagccttacc tgcagacgag
     3181 aacggcggag cccgagcgcg aatggcgcca gtcggaggag gaccgtgcga aactggacgg
     3241 ctactacgaa tgcattcttt gcgcctgctg ctccacctcg tgcccgagct attggtggaa
     3301 ttccgagcgc tatctgggcc ccgctgcgct cctccaagcc tggcgctggc tcgccgacag
     3361 ccgcgacgaa gcgagaggcg agcggctcga caagctcgaa gattcgttcc gcctctaccg
     3421 gtgtcacaca atcatgaact gcgcccagac ctgccccaag ggcctcaatc ccgcccgcgc
     3481 catcggcgag atcaagcaga tgctggtgaa gcggagcatt taaggggcgc tctgttcacc
     3541 ggagcgctgc gcacgctttg cagttcggtt ttggtggaaa tggcagcaat cggaatcgaa
     3601 tagcgtttcg atttatcccg cgggtgcgag tcccgcgcgg aggcggagta tgctccacaa
     3661 caccgcgctc gcggcgcggg tcagccggcc ttgcggattg gcgccgaccg atttggcgac
     3721 catctggacc agcttgccga tatgatggcg gcggtagagt cccatcgcgc gcgcctgata
     3781 ggcgcgagca aagcgtctgc gttggtatgg aatgttcgct tcagccgaag ccggcacgtt
     3841 ggcgcagtaa taggcatagg ccagttcgtc gtcgctcgat tcgttgacgc gcgtggccgc
     3901 taccagaatc ctgcgccacc aaggcagttc gggctcatcc tgggcgtgaa gcgtatcaag
     3961 gaagagcttg taatgccgga attcatcggc ggcgatccgg cccgcgacct ccttgagaac
     4021 cggttcgtcg gccgcatcgc ggatggcgct gtagaaggaa gatgtccctg attcgacgac
     4081 gcagcgggcg atcatctcgc cgcggcggct gccccgcaca gaatccggat cgtccgcggt
     4141 gaagtgcggc ggcctgtagc cggcccgaaa gcgtgcgaaa gctgattcga aatcgaagga
     4201 cgggtctgca atttccgccc aacggccgag cgccagccca tgttgaacct cttcgcggcc
     4261 ccaacgctcg atatccgaaa ctgtcttggc gcctgcgtcg cggaacacgc gctgcagata
     4321 ggagacgtag tcgggggcat tgagctcgac cagggcggca ctcttcacgg ccgcaagcag
     4381 ccccccctcg accctggcgg ggtcgaagct gtcccatgga atgtcgtcca gcgtccagcc
     4441 cgacgcatag accacagcac cgatcatccc gcgtctatcc gtagccaccg aagtcccatg
     4501 ccttatacca gaaccggatg cattcggaaa atcgtcagag ggcggagcga agctggcgca
     4561 gatggtcgag ctgctcggcg acgcgggctc ggtctccttc atttttagcc aaaacaagat
     4621 cttcttcggt gtttttgatt ctctggtcga gggttgcggc gtcgattgcc gtcatcggaa
     4681 ccgcctcttc ggccaggacg gtcagcttgc ggggattggc ttcggcaaag cctcccagga
     4741 cgaaaaaccg cacgtcgccg cattcgccgc cgcccctgat ttccacgacg ccggggcgca
     4801 gcgtcgagat cagcggcatg tgaccggcga gcacgccaaa atcgccctcg cttcccggca
     4861 ccgtgaccat gtcggcctcg tcggacagca gcagccgctc gggcgagaca atatcgaagg
     4921 cgatcttgtc ggtcatgtca ggccgcttcg gcggcgagct tctgcgcctt ggcgaccgcg
     4981 tcctcgatcg tgcccaccat gtagaacgcg gcctcgggca ggtgatcgaa ttcgccgttc
     5041 accaccgcct tgaagctgcg gatcgtgtct tcgagcttga cggttatgcc cgacatgccg
     5101 gtgaacacgg ttgcggcata gaagggctga gagagaaacc gctcgatctt gcgggcgcgc
     5161 gcgacggtca gcttgtcctc ctcggacaat tcatccatgc cgagaattgc gatgatgtcc
     5221 tgcagagctt tgtagcgctg gagtatctgc tgcacctggc gcgcgacgtt gtaatgctct
     5281 tcgccgacga cgcgcggatc gagcaggcgc gaagtggaat ccagcgggtc cactgccggg
     5341 aagatcgcct tctcgaagat ggcgcgtgac agaacggtgg tcgcgtcgag atgcgcaaaa
     5401 gatgtcgcgg gagcagggtc ggtcagatcg tcggccggca catagatggc ttgcaccgag
     5461 gtgatcgaac ctttcgtcgt cgtcgtgatg cgctcctgca atgtgcccat gtcggtggcg
     5521 agggtcggct ggtagccgac cgccgaggga atgcggccga gcagcgccga gacttcggag
     5581 cccgcctggg tgaagcggaa aatgttgtcg
//
LOCUS       DASZMC010000111         5173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_607763,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000111 DASZMC010000000
VERSION     DASZMC010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607763"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VG274_02150"
     CDS             complement(<1..339)
                     /locus_tag="VG274_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWE05479.1"
                     /translation="MFEARVIGNVSKPGLYREIAQQLQALVEGEPDPVANAANAAALL
                     FQTLPDVNWTGFYFLRDGNQLVVGPFQGRPACVRIPVGQGVCGTAAARWESIMVADVE
                     DFPGHIACDTA"
     gene            438..1907
                     /locus_tag="VG274_02155"
     CDS             438..1907
                     /locus_tag="VG274_02155"
                     /inference="COORDINATES: protein motif:HMM:NF005702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWE05480.1"
                     /translation="MDEGLFAQMRAAMPDGSRPFLRTSDGRRFSYDDMLIRSGQFANC
                     LHAAGVRPGDRLAVQVEKSAEALLLYLACLRTGAIYVPLNPAYTSAETPWFLTDAEPI
                     CFVAAPEKAEAIQTLLGDTTALLTLGCDGRAGSLTERAGSIASGFHDAVRAGDDVAAI
                     LYTSGTTGRPKGAMLTQRNLASNALALRDLWRFSPRDVLLHALPLFHTHGLFTATNTS
                     MMAGASMILLPRFDLDAVFAHLPEATTMMGVPTFYTRLLADSRLVRARVAHMRLFISG
                     SAPLLPETHRAWLDRTGHAILERYGMTETGMIASNPCDGERIAGTVGRPLPGVDLRIA
                     DPQDGSPLPSGQVGMIELRGPNVFRAYWRKPEQTAALFREDGFFVTGDLGTIDSSGYL
                     RIAGRATDLVISGGFNVYPREIETEIDALPGVAESAVIGVPHPDLGEGVTAIVVVKSG
                     TELREVTCLPRLASALPDTNCRGAYCSSMRYRAMPWARS"
     gene            2183..3832
                     /locus_tag="VG274_02160"
     CDS             2183..3832
                     /locus_tag="VG274_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWE05481.1"
                     /translation="MKLKTILLGAAAFAWLVGPAFSQVGTSAPASVDPPRKHLHHANT
                     LEERLDRMERIIQEQQAEIRDLKAQTGQASGGASATAAAAPAQPQPEVSAAQFEALQN
                     QIYEQAAENKSHAVVTLKKGRPTIATPDGKYSLSIISLVQADAATFDDSSVTGPKGTP
                     VLSRSGFNFRRAQIGVQGVLGSDFGYKFNYDFGGAGGQESGAENIITGTTPPTVAVNP
                     SGARIQSAYVTYKGILDPFTFKIGAFPTPANLADATGADDLLLNERASPAQLSRGLDA
                     DDGRTGLGFQGNGSIWYTSTYLTSDTAGKGQIEGQEAFVGRVALAPIQDPDSNFNIHL
                     GGNVGDVFHPEGVNPASCTTTAPACYLVSFSDRPELREWNVSWISSGNVNAKSAYSAG
                     VEAAVSYGPLLLEGENFWYGISRRDASTGQTNPTFSGWYIEGSWVLTGEPRTYSMASA
                     AFLRPAPASDFDPAHDSWGAWEFAARYSDTDLNYNVNSLVAADQVRGGDQKIWSAGFN
                     FYPNYNLKFMLDWQNIDISRPWSGAFGTRYNAFSLRTQVSL"
     gene            complement(3923..4327)
                     /locus_tag="VG274_02165"
     CDS             complement(3923..4327)
                     /locus_tag="VG274_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05482.1"
                     /translation="MSGLLFRAIIIVAVILACAIGLTAAFALCVAADYFAFATLLSPP
                     LAALAAAGTALLFCALAVFLGQFALARMRARARKTLFFRIGQILGETFGTDILNLADR
                     YPTRSIAAALFAGFALGFSPKLRSALRDFLRR"
     gene            complement(4348..4626)
                     /locus_tag="VG274_02170"
     CDS             complement(4348..4626)
                     /locus_tag="VG274_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05483.1"
                     /translation="MSATESDVQALSDELKQLRAEFTKLGQLLESAARNAGSEAAQAA
                     RATGERAWSEVKKSADDLTQRIEARPVTSAATAFGIGVVLGLLFGGRR"
BASE COUNT          962 a         1647 c         1611 g          953 t
ORIGIN      
        1 ggccgtgtcg caggcgatat ggccgggaaa gtcctcgacg tcggcgacca tgatcgactc
       61 ccatcgcgcg gccgccgtgc cacatacgcc ttggcccacg gggatgcgga cgcaggcggg
      121 tctgccctga aagggtccga caacgagctg gttgccgtcg cgcagaaaat agaatccggt
      181 ccagttcaca tccggaagcg tctggaacag caaggcggcg gcgttggcgg cgttggcgac
      241 gggatcgggc tctccttcca ccagcgcttg gagctgttgg gcgatttccc tgtacaatcc
      301 cggtttggaa acatttccga tcacgcgtgc ttcgaacatt ttcttcttcc cggattgcgg
      361 tttcggcaga gtcgtgccga gacattcata ttgggtgcaa gccgcaatgg ccgcaacgtt
      421 gagcgatcac gccgcaaatg gatgaaggtc tcttcgcgca aatgcgggcg gccatgccag
      481 acggcagccg gccgtttctc cgcacttcgg acgggcggcg attctcctac gacgacatgc
      541 tcatacgctc gggccagttc gccaattgcc tccacgcggc aggcgttcgg cccggcgaca
      601 ggcttgccgt ccaagtggag aagagcgccg aagcgcttct gctgtacctg gcttgcctga
      661 gaacgggcgc gatctatgtg ccgctcaacc ccgcctacac gtctgccgaa acgccctggt
      721 tcctcaccga cgccgaaccg atctgcttcg tcgcagcgcc ggagaaggcg gaagcaattc
      781 agacgctgct cggcgacaca acggcgcttc tgactctcgg ctgcgacggg cgcgccggct
      841 ccctcacgga gcgcgccggt tcaatcgcga gcggcttcca cgatgcagtg cgtgcgggcg
      901 acgacgtggc ggccattctc tatacgtccg gcacaacggg gcgtccgaag ggcgccatgc
      961 tcacgcaacg caacctcgct tcgaatgcgc tcgcgctgcg cgatctgtgg cgattctcgc
     1021 cgcgcgatgt tctgctgcac gctctgccgc tcttccacac ccacggtctc ttcaccgcca
     1081 ccaacaccag catgatggcc ggcgcgtcca tgatcctgct gccgagattc gatctcgatg
     1141 cggtgttcgc ccacttgccc gaggcaacaa cgatgatggg cgttccgaca ttctacacca
     1201 gactgcttgc cgactcgcgg cttgtgcgcg cccgtgtcgc gcatatgcga ctgttcattt
     1261 caggctccgc gcctctcttg ccggaaaccc atcgggcgtg gctcgatcgc accggtcacg
     1321 ccattctcga gcgctacggg atgacggaga ccggcatgat cgcgtcgaac ccctgcgacg
     1381 gggaacgaat cgccggcacg gtcgggcgtc cgctccccgg ggtcgacctg cgcattgccg
     1441 atccgcagga cggttcgccg ctgccgtccg ggcaggttgg aatgatcgaa ttgcgtggcc
     1501 cgaatgtctt tagggcctat tggcgcaagc cggaacagac cgcagctttg ttccgcgaag
     1561 acgggttctt cgtcaccggc gatctcggaa cgatcgattc ttcgggttac ctgcggatcg
     1621 caggccgcgc aacggacctc gtgatcagcg gcggcttcaa tgtctatccc agggagatcg
     1681 agacggagat cgatgcgctt ccgggggttg ccgaatcggc agtgatcggc gtgccgcatc
     1741 ccgatttggg tgagggcgtg accgcaatcg ttgttgtaaa gagtggcacg gagctccggg
     1801 aggtgacgtg cttgccgcgc ttggcgagcg ccttgccaga tacaaattgc cgaggcgcgt
     1861 attgttcgtc catgagatac cgcgcaatgc catgggcaag gtcatgaaga acgttctgcg
     1921 cgaagtgcat cgctccctgt acgcccgcgc ggcaagcgga aggctatcct agattgcgga
     1981 gaagaggggc aagaggacgt cacgaatgtg tcacaagagt ttcgtacaca tgatgcgccg
     2041 ctgacatttg cggagagcag tttgcctcga cagggcgggg atctgtgcat gaattgccgg
     2101 ggttgttgtc cagaatagcc attgacttga ttgtgtaagt cgtgttttag gtgtttccag
     2161 cgtactgcga gcggggaaaa tcatgaaact gaagaccatt ctgttgggtg cggcggcatt
     2221 tgcatggctc gttgggccgg cattttcaca agtagggaca agcgccccgg cttctgtcga
     2281 tccgcccagg aagcacctcc accacgccaa tacgctggaa gagcggctcg accgcatgga
     2341 gcggatcatc caggagcagc aggccgagat tcgcgacctg aaggcccaga ccggacaggc
     2401 cagtggtggc gcgtccgcca cggcggcagc cgcgcctgcg cagccccagc ccgaggtgag
     2461 tgccgcacaa ttcgaggcgc tgcagaacca gatttatgaa caggcggcgg agaacaagag
     2521 ccacgccgta gtcaccctca aaaagggcag accgacgatc gcgacgcccg acgggaaata
     2581 ttcgctgtcg atcatttcgc tcgtgcaggc ggatgcggcg acgtttgacg attcttcggt
     2641 caccggcccg aaaggcaccc ccgtgctcag ccgcagcggc ttcaacttcc gccgcgcgca
     2701 gatcggagtc cagggcgtgc tgggcagcga tttcggctac aagttcaact atgatttcgg
     2761 cggtgcgggc ggccaggaga gcggcgcgga aaacatcatc accggaacga cgccaccaac
     2821 cgtcgccgtc aatccaagcg gcgcgaggat tcagtcggct tacgttacct acaagggcat
     2881 tctcgatcct ttcaccttca agattggcgc ctttcccacg cctgcgaatc tcgcagacgc
     2941 aaccggggct gacgatctgc tgctgaatga gcgcgcttct cccgcgcagc tctcgcgcgg
     3001 ccttgatgcc gacgacgggc gcaccggact cggattccag ggcaatggca gcatctggta
     3061 cacttcgacc tatctgacct ccgacactgc cggaaaggga caaatcgaag gccaggaggc
     3121 cttcgtcggc cgcgtcgcac tcgcccccat tcaggacccc gacagcaatt tcaacattca
     3181 cctcggcggc aatgtcggcg atgtcttcca tcccgaagga gtgaatcccg cgtcctgcac
     3241 gacgacggcg ccggcttgct atctcgtgtc gttctcggat cgccccgagc tgcgcgaatg
     3301 gaacgtctcc tggatcagca gcggcaacgt caacgccaaa tccgcttaca gcgccggcgt
     3361 cgaagcagcg gtcagctatg gcccgctcct gctcgaggga gaaaatttct ggtacggcat
     3421 cagccgccgc gacgcgagca ccggccagac caatcccacc ttttcgggct ggtatatcga
     3481 aggcagctgg gtgctgaccg gcgaaccgcg cacctacagc atggcgagcg cggcgttcct
     3541 gcggccggcg cccgccagcg atttcgaccc cgcccacgat tcgtggggcg cctgggagtt
     3601 tgcggcgcgc tacagcgaca ccgatctgaa ctacaacgtc aattcgcttg tcgcggccga
     3661 tcaggtgcgt ggcggcgacc agaagatctg gtcggcaggg ttcaacttct atccgaacta
     3721 caatttgaaa ttcatgctcg actggcagaa catcgacatc agccggccct ggtcgggcgc
     3781 gttcggcaca agatacaatg cgttctccct gcggacccag gtctcgctct gagagcagct
     3841 ctaatcgcag tgcaccgcgt ccggccacgc tccgccacct gcgccggccg cacgctctcg
     3901 cgcgtcgcga cgtgaccagg catcagcggc gcaggaagtc gcgtagcgcg ctgcgcaatt
     3961 tcgggctgaa tccgagggcg aagccggcga atagcgctgc ggcaatcgaa cgcgtgggat
     4021 agcggtctgc cagattcagg atatctgttc cgaaggtttc cccaaggatt tgcccgatgc
     4081 ggaagaacag cgtctttcgc gccctcgccc gcattcgcgc gagcgcgaac tggccaagaa
     4141 atacggcaag ggcgcagaac agcagagcgg ttcccgccgc ggccagggcc gctagaggcg
     4201 gcgaaagaag cgtcgcgaag gcgaaataat ccgcggcgac acagagggcg aaggccgccg
     4261 tcaacccgat ggcgcaagcc aggatgacag cgacgatgat gattgcgcgg aacagaaggc
     4321 cgctcatctg accggccgcc ggatcggtta gcggcggccg ccgaacagca gccccagcac
     4381 gactccgatg ccgaaggcgg tggccgcgga ggtcaccgga cgggcttcga tccgctgggt
     4441 cagatcgtca gcgctcttct tgacttcgct ccaggcgcgc tcgccggtcg cgcgtgccgc
     4501 ctgcgcggct tcgctgcctg cgtttcgggc agcagattcc agaagttggc ccagcttcgt
     4561 gaattccgca cgcagctgct tgagttcgtc ggaaagagcc tgaacatccg attcggtcgc
     4621 agacatgatc tatctcctct ggcgagcggc tgtatatcac gggaaaatac gccggctcca
     4681 cccggcttcc cgcgcggaac cggggaatga acctaatccc gtttcgtttc ccaagggttg
     4741 cggcgacggg cgcgccccgc tggtagcact gtcccacggg caacgctaac ctcgccgatc
     4801 cgccgggcct cccttcgtga attttttttg gaactgcgca tggcgtctca gagtccccag
     4861 gtttcgtcgg cagaacccgc aaggcgacac tgagccatcc cccatgtcgc gtccgtcgcc
     4921 ctggcgatcc tcgcctatct ggcaatccat tccggcacgg gagattccga cctttttctc
     4981 gcgcttgccg cggccggcgg cgcgatgact tggcccgggc tgtgggtcga gccgtggttc
     5041 cgaaagagga aagcgacctg gatccggacc gatcggcttg ctctgggccg cgatgccatt
     5101 gcatcttggc gcgagcgtgc ccccgcacca tatgttaacc ctgtacggat tttcgcccag
     5161 atcgttgtga gat
//
LOCUS       DASZMC010000112         8503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1965964,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000112 DASZMC010000000
VERSION     DASZMC010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965964"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1173
                     /gene="rpoH"
                     /locus_tag="VG274_02175"
     CDS             277..1173
                     /gene="rpoH"
                     /locus_tag="VG274_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HWE05484.1"
                     /translation="MSSRGVPALQGEGGLSRYLSEIRKFPLLEPEEEYMFAKRWREHD
                     DPEAARRLVTSHLRLVAKIAMGYRGYGLPVSEIVAEGNVGLMQAVKRFEPDRGFRLAT
                     YAMWWIRAAIQEYILRSWSQVKMGTTAAQKKLFFNLRKVKSRIGALEEGDMRPEQVRE
                     IADKLAVPEEEVVNMNRRLAAPDSSLNAPLRSDSESEWQDWLVDDTLDQESRLADAEE
                     MGERHDLLTPALGELSERERDIIEARRLRDEPVTLEELSQKYGVSRERVRQVEVRAFE
                     KLQQQMKRSLSDKRRHTLAVAG"
     gene            1345..2148
                     /locus_tag="VG274_02180"
     CDS             1345..2148
                     /locus_tag="VG274_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWE05485.1"
                     /translation="MKLKLWQIDAFAEKPFEGNPAAIVPLSGWLDPRLLQQIANENNL
                     AETTFLVRHGDGRYGLRWFTPTTEVDLCGHATLASAWLLFAELDPELMSVAFETRSGT
                     LVVQRGENGRHAMSLPSDIVVPVDDASLPAKMGEALSTLAPLEVFQGRNLLAVFADAS
                     TIRAINGPGEIGRVIPQDKGMIATAKGDGGYDFVSRYFAPHHGVPEDSVTGSAHCALT
                     PFWAARTGKATLRARQASPRGGDLVCTDAGGQTILSGACVLYMRGEIDV"
     gene            complement(2156..2596)
                     /locus_tag="VG274_02185"
     CDS             complement(2156..2596)
                     /locus_tag="VG274_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HWE05486.1"
                     /translation="MPLRSTSRGDPDIELGIATDKVCHLIVKARAFDAKVADADPDSG
                     SNPTDDAMADVLESGTGDPVLHELSAFIRGLDEDEQMSLVALAWIGRGTFDASEWQQV
                     LETARTQHRHRVAEYLLSLPLLGDYLEEGLAAFGKDCADFNARI"
     gene            2688..3866
                     /locus_tag="VG274_02190"
     CDS             2688..3866
                     /locus_tag="VG274_02190"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF002841.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HWE05487.1"
                     /translation="MTEKKPLLRPSRPFFSSGPCAKRPGWVLEGLRGALVGRSHRSPP
                     GRAKIREALGRTRTLLGIPPDYRIVLTPASDTGAVELAMWSLLGPRPVDAFVWESFGT
                     EWMTDAIDQLKLKNTRVFKAGYGALPDLNAADPRHDIVFVWNGTTSGVRVPDGEWIAR
                     DRDGLTICDATSAAFAMELPWDKLDATTFSWQKSLGGEAAHGMLVLSPRAMARLGSHA
                     AEWPLPKIFRLKKDGKLIDGLFEDETINTPSMLAVEDYLDALGWAESIGGLKTLLART
                     CANFASLSQWVQKSRWTNFMAAEERFRSNTSVCLTIVDSRFAAMERSAQASTVANLTR
                     LLEQEQIAFDIGAHRTAPPGLRIWCGPTVEAEDIASLTLWLDWAWERTKPPSPTDERQ
                     "
     gene            3863..4435
                     /locus_tag="VG274_02195"
     CDS             3863..4435
                     /locus_tag="VG274_02195"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWE05488.1"
                     /translation="MTPQIALNYLWAFWVLSWLAAALWSAPSAKRPVFGSEILYRVVT
                     LLGAALLFGVTERRAPMRLSSFSESADWALFGVCVGAFVFCWWARIHLGRLWSAWVSK
                     KQGHRIIDTGPYRVVRHPIYTGLILAALATAAMKGTSIALGGAAIMAVGFWIKARLEE
                     RFLCQELGAEAYSVYRRRVPMLIPLGPKSA"
     gene            4517..5812
                     /locus_tag="VG274_02200"
     CDS             4517..5812
                     /locus_tag="VG274_02200"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWE05489.1"
                     /translation="MANVAVIGAQWGDEGKGKVVDWLSARADVVVRFQGGHNAGHTLV
                     IDGKTFKLSLLPSGVVRPGKLSIIGNGVVVDPWAFADEVERLAAQGVLVSPQSLLVAE
                     NAALILPLHRELDFSRESSDSVQRVGTTMRGIGPAYEDKVGRRALRVIDLADQPSLPP
                     KIERLLAHHNALRRGMGAAEIDSAALATALAAIAPRILPYCGSAWLRLDAARRDGKRV
                     LFEGAQAVLLDVDHGTYPYVTSSNTVAGQAAAGSGCGPRDVGYVLGIAKAYTTRVGEG
                     PFPTELCDAIGEGLGTRGAEFGTVTGRKRRCGWFDAVLVRQTVITGGIDGLAVTKLDV
                     LDGMPEIKLCTGYTLDGRDTAHLPAEAGAQARIKPIYETLEGWSESTRGARSWAELPA
                     TAIKYIRRVEELVGAPVTLLSTSPEREDTILVRDPFVDR"
     gene            5840..6817
                     /locus_tag="VG274_02205"
     CDS             5840..6817
                     /locus_tag="VG274_02205"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWE05490.1"
                     /translation="MFKTRFTEMFGVEAPITCGGMTRVGMVELIAAVANAGALGFITA
                     HTAGRPEDLAKAVNRCRGLTDKPFGVNLTILPSITPIPYDEYREAIIESGVRIVETAG
                     NNPQPHLPRFREAGVKVIHKCTSVRHAVKAQSIGVDCVSIDGFECAGHPGEDDVGGLI
                     LFPATADKLTIPIIASGGMADARGLVAALALGCDGANMGTRFMATVEAPIHENVKKQI
                     VANDERSTNLIFRTLHNTARVAKNTISDEVVAIEKRGNATFADVKDLVAGTRGAIVLD
                     QGEMDAGIWSAGQTQSLIHDIPTCAELVSRIMREAEEIIRQRLGAMVAR"
     gene            6946..7197
                     /locus_tag="VG274_02210"
     CDS             6946..7197
                     /locus_tag="VG274_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05491.1"
                     /translation="MARGVVRVEGQAPKKDADLIRALARTLRGKAENAKALRSTLTKA
                     LVHPEAKNAFDVFGSELPEEFFAGIFGWPHQRSRRKIVL"
     gene            complement(7323..8126)
                     /gene="thiD"
                     /locus_tag="VG274_02215"
     CDS             complement(7323..8126)
                     /gene="thiD"
                     /locus_tag="VG274_02215"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWE05492.1"
                     /translation="MKAAPARLLIVAGSDSGGGAGIQADIKTATALGVYAMTAVTAVT
                     VQDTARVHAIHLIPPSIIAEQIACVLADIGADAIKIGMLGSADAVDAVADALEAHGSG
                     IPLVLDPVLVSSSGTSFLSGDAVTRMKDRLLPLTALLTPNIPEAECLTGAVLGSRDDI
                     LHAADALRRMGADAVLIKGGHASGTPIRDILVSRDGIEEFEYPRVPTRATHGTGCTLA
                     SAIACELAQHRPLADAVRSARSFVQAALETAPDFGRGQGPLNHLRSIRH"
     gene            complement(8123..>8503)
                     /locus_tag="VG274_02220"
     CDS             complement(8123..>8503)
                     /locus_tag="VG274_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05493.1"
                     /translation="FNVGGEQSGHIVLRDFCTTGDGVIAALQVLGVLAHEKRPASEAA
                     HIFEPLPQLVENVRYKNGSPLKDPRVVERIKDCEARMNGTGRLLVRKSGTEPVIRVMA
                     EADDERLVRSVVSDVVAALQEVVA"
BASE COUNT         1583 a         2672 c         2723 g         1525 t
ORIGIN      
        1 cccgcatcag gcgctgcatg cccgcctgct cggcttcaaa cacccgacga gccagaaaca
       61 gatgcgcttc gaggcgccat ggcccgccga ttttgccgga ctggtcgcaa gcctgcgcgc
      121 cttgcaagaa cgctgaggga acttacattg tttaagtttg cggggaattt cggcactatc
      181 gcccgcggac cgaacgacct gcctgggcgt tcccagaagg cataaggcgg tgtcgtgcgc
      241 gcttgggcgc acggaacgcc agacgggacc ttgaacatga gcagtcgcgg cgtgccggcg
      301 ctccagggcg aaggcggcct ctccagatat ctgtcggaaa tccggaagtt cccgcttctg
      361 gagccggaag aagaatacat gttcgccaag cgctggcgcg aacacgacga tcccgaggcc
      421 gcccgccgcc tcgtcaccag ccatttgcgc ctggtggcga agatcgccat gggctatcgc
      481 ggctatggcc tgccggtttc ggaaatcgtt gccgaaggca atgtcgggct gatgcaggcc
      541 gtgaagcgtt tcgagcccga ccgcggcttc aggctcgcca cctatgccat gtggtggatc
      601 cgcgccgcga tccaggaata catcctgcgc tcctggagcc aggtgaagat gggcacgacg
      661 gccgcgcaga agaagctgtt cttcaatctg cgcaaggtga agagccgcat cggcgcgctt
      721 gaggagggcg atatgcgccc cgagcaggtg cgcgagatcg ccgacaagct cgccgttccc
      781 gaggaagaag tggtgaacat gaatcggcgc ctcgcggcgc ccgattcctc gctcaacgcg
      841 ccgctccgct cggattccga atcggaatgg caggactggc tggtggatga cacgctcgac
      901 caggaatcgc gtctggccga cgcggaggag atgggcgagc ggcacgatct tctcacgccg
      961 gcgcttggcg aactcagcga gcgcgagcgc gacattatcg aggcgcgtcg gctgcgggac
     1021 gaaccggtga cgctcgaaga gctgtcgcag aaatacggag tgagccgcga gcgcgtacgg
     1081 caggtcgagg tgcgcgcatt cgaaaagctg cagcagcaga tgaagcgctc tctctcggat
     1141 aagcgccgtc acacgctggc ggtcgccggc tgacggcatt cccgcgcagg gccgccatgc
     1201 gccggcgctt cttcatctac agcgatttcc gtttatgttg aaccaaacat gtcatgttgg
     1261 ggttgtgatt caatttgatc ggacggtgct ccaagtacat cccgcacgat attgtgtctg
     1321 tccccattgg ttcgcggcgg tgcgatgaaa ctcaagctct ggcaaatcga cgccttcgcc
     1381 gaaaagccgt tcgaaggaaa tccggcggcg atcgtgcccc tttcgggttg gcttgatcca
     1441 cggctcctgc agcagattgc caatgagaac aacctcgcgg aaaccacctt tctcgtgcgg
     1501 cacggcgatg gccgttacgg attgcgctgg ttcacaccca cgacggaggt ggatctctgc
     1561 ggccacgcga cgctggcaag tgcatggctg ctgttcgccg agctcgatcc ggagttgatg
     1621 tcggtcgcgt tcgagacgcg ctccggcacc cttgtcgtgc agcgcggcga aaacggccgg
     1681 cacgccatgt cgctgccttc ggacatcgtc gtgccggttg atgatgcttc actgccagca
     1741 aaaatgggcg aggctctctc gacgctcgcg cctttggaag tatttcaggg gcgaaacttg
     1801 ctcgcggtgt tcgccgacgc atcgacaatt cgcgccatca acgggccagg cgaaatcggc
     1861 cgcgtgatcc cgcaggacaa ggggatgatc gcgaccgcga aaggcgacgg cggatacgat
     1921 ttcgtctcgc ggtatttcgc gcctcatcat ggcgtgcccg aggattcggt gacgggctcg
     1981 gcgcattgcg cgctcacgcc cttttgggcg gcgagaaccg gcaaagcgac gctgcgcgcg
     2041 cgccaggcga gtccgcgcgg aggcgatctc gtttgcaccg acgcgggtgg acaaacaatt
     2101 ctttcgggtg cgtgcgtgct ctacatgcgc ggagaaatcg acgtttgacg aaaaactaga
     2161 tccgcgcgtt gaaatccgcg caatccttcc cgaacgccgc gagtccctct tcgagataat
     2221 ctccgagtag cggcaggctg agcaggtatt cggccacgcg atgccggtgc tgggtgcgcg
     2281 cggtctcgag tacctgctgc cactcggaag cgtcaaacgt tccccggcct atccaggcaa
     2341 gtgcgacgag gctcatctgc tcgtcttcgt cgagcccgcg aatgaaggcg ctcaactcgt
     2401 gcaggaccgg atcgccagtc ccgctctcga gaacgtcggc catcgcgtca tcggtggggt
     2461 tcgatcccga gtccggatcg gcatcggcca ccttcgcgtc gaaggcccgc gccttcacga
     2521 tgaggtggca aaccttgtcg gtcgcgatcc ccaattcgat atcgggatct ccacgcgatg
     2581 tggatcgaag cggcatggac acagactcct tctcgaccct atgaaaaacg atccggcgcc
     2641 acgcgatgtt ccggcgcctc ctcttcggat cgcatcagga ttgtgccatg acggaaaaaa
     2701 aaccgctttt gcgcccatca cggccattct tctcatcggg accgtgcgcg aaacggccgg
     2761 gctgggtcct cgaaggtctg aggggcgcgc ttgtcggccg gtcgcaccgc tcgccgcccg
     2821 gcagagcgaa aatccgtgaa gcacttgggc gaacaagaac gctgctgggc ataccccccg
     2881 actaccggat cgtgctgacg cccgcatccg acaccggcgc cgtcgagctc gccatgtggt
     2941 cgctcctggg gccgcggccg gtcgatgcgt tcgtgtggga gagctttggc acggagtgga
     3001 tgaccgacgc gatcgaccaa ttgaagctga agaacacgcg ggtcttcaaa gcgggatatg
     3061 gagcgctgcc cgatctcaat gcggcggatc cgcgacatga cattgtattc gtctggaacg
     3121 gcacgacctc cggcgtgcgc gttccggacg gcgagtggat cgcgcgcgac cgcgacggtc
     3181 ttacgatctg cgatgcgacg agtgcggctt tcgcgatgga attgccgtgg gacaagctcg
     3241 atgccacgac gttctcctgg cagaaatcgc tgggcgggga ggctgcgcac ggcatgcttg
     3301 ttctctcgcc gcgcgccatg gcgaggctgg gctcccacgc tgcggaatgg cctctgccaa
     3361 agatcttccg gctcaagaag gacggcaaac tgatcgatgg gctgttcgaa gacgaaacga
     3421 tcaatacgcc ctccatgctt gcagtggagg actatctgga tgcgctcggc tgggcagaat
     3481 cgatcggcgg cctgaaaacg ctgctcgcgc gcacctgcgc caatttcgcc agcctttcgc
     3541 agtgggtgca aaaaagcaga tggacgaatt tcatggccgc ggaagagcga ttccggtcca
     3601 atacgtcggt ttgtctcacg atcgtcgact cgcggttcgc agcgatggag agatccgcgc
     3661 aggcatccac ggttgcgaac ctcacccgtc tcctggagca ggagcagatc gctttcgaca
     3721 tcggcgcgca ccgcaccgct ccaccgggct tgcgcatctg gtgcggaccg acggtcgaag
     3781 cagaagacat cgcttcgctg acgctgtggc tggactgggc ctgggaacgc accaagcccc
     3841 cctccccaac ggacgagagg caatgacacc gcaaattgcg ctgaactacc tttgggcgtt
     3901 ctgggttttg agctggctcg ccgccgcctt gtggtccgca ccctcggcga agcgcccggt
     3961 cttcggttcc gaaatactct accgggtcgt caccctgttg ggcgccgcgc tgctgttcgg
     4021 tgtcacggaa cggcgcgccc cgatgcgcct atcgtcgttc agcgagagtg cagactgggc
     4081 attgttcggc gtctgcgtgg gcgcatttgt attttgctgg tgggctcgca tccatctcgg
     4141 acgcctgtgg tcagcttggg tgtcgaagaa acaggggcat cgcatcatcg acaccggccc
     4201 ttatcgcgtc gtgcgccatc ccatctatac cgggctgatc cttgccgccc ttgcgactgc
     4261 ggcgatgaag ggcacgagca tcgcgcttgg gggcgccgcc atcatggcgg tcggcttctg
     4321 gatcaaggcg cggctcgagg agcgattcct gtgccaggaa ctgggcgcgg aggcctacag
     4381 cgtctatcgc cgccgcgtcc cgatgctgat tccgttgggc cccaaatcgg cctgacgccg
     4441 cccttcattg cccgtatagt cggccaccgt gcccggcgaa tcggcgggcg gagaggaaac
     4501 catccgggta cggacaatgg cgaacgtggc ggtgatcggc gcccagtggg gcgacgaggg
     4561 aaaaggcaag gtcgtcgact ggctgtcggc gcgcgccgat gtggtcgtgc ggttccaggg
     4621 cggccacaat gccggacaca ccctcgtcat cgacggcaag accttcaagc tcagcctgtt
     4681 gccctcgggc gtggtgcgcc cgggcaagct gtcgatcatc ggcaatggcg tcgtggtcga
     4741 cccgtgggcc tttgcggacg aagtcgagcg cctggctgcg caaggcgtgc tcgtatcgcc
     4801 ccaatcgctc ctggtcgccg aaaacgccgc gctgattctg ccgttgcatc gcgagctcga
     4861 tttttcacgc gaatcgtcgg acagcgtgca gcgcgtcggc accacgatgc gcggcatcgg
     4921 tccggcttat gaagacaagg tcggacggcg cgcgttgcgc gtcatcgatc tcgccgatca
     4981 gccttcgctg ccgccaaaga tcgaacggct cctcgcacat cacaatgcgc tgcgtcgtgg
     5041 aatgggcgcc gcggagatcg atagcgccgc tctcgcaacc gcgcttgcgg cgatcgcgcc
     5101 ccgcatcctt ccctattgcg gatcggcatg gctcaggctc gacgccgcgc gccgggatgg
     5161 aaaacgcgtg ctgttcgagg gcgcccaggc cgtgcttctc gacgtggacc acggaacata
     5221 tccctacgtc acctcgtcca acacggtcgc cgggcaggct gctgcgggga gcggctgcgg
     5281 acctcgcgat gtgggttacg ttctgggcat cgccaaggct tacaccacgc gcgtcggaga
     5341 aggtccgttt cccaccgagc tttgcgacgc gatcggcgaa gggctgggca cgcggggcgc
     5401 agagttcggg actgtcaccg gccgcaagcg gcggtgcggc tggttcgatg ccgtgctcgt
     5461 cagacagaca gtgattaccg gcggcatcga cggcctggcg gtgacaaagc tcgacgtgct
     5521 cgacggtatg cccgaaatca aactctgcac cggctatacg ctcgacggac gcgacaccgc
     5581 gcatctgccg gccgaggcag gggcgcaggc gcgcatcaag ccgatctacg aaacgctcga
     5641 aggctggagc gagtccaccc gcggcgcgcg ctcctgggcg gagttgccgg cgacggcgat
     5701 caaatacatc cggcgtgtgg aggagctggt cggcgcaccg gtcacccttc tctccacaag
     5761 tccggaacgc gaagacacga ttctcgttcg cgatcctttc gtcgatcgct gaaggccttt
     5821 ccgtaagctg ggagcatcga tgttcaaaac gcgattcacg gaaatgttcg gcgtcgaggc
     5881 gccgatcacc tgcggcggca tgacccgcgt tgggatggtg gagctgattg cagccgtcgc
     5941 gaacgcgggc gcactcgggt tcatcaccgc gcacacggcc ggacggcctg aggatctggc
     6001 gaaggccgtg aaccgttgcc gcggcctgac cgacaagccg ttcggcgtga acctgacaat
     6061 cctgccgagc attacaccga tcccctatga cgagtatcgc gaagccatca tcgagagcgg
     6121 cgtgcgcatc gttgaaaccg caggcaacaa cccgcagccg catctgcctc gcttcaggga
     6181 agccggagtc aaagtgatcc acaaatgcac gagcgtgcgg cacgcggtca aagcgcaatc
     6241 gatcggcgtg gactgcgttt cgatcgacgg attcgaatgc gccggccatc ctggcgagga
     6301 cgatgtcgga ggcctgatcc tctttcccgc gaccgcggac aagctcacca ttccgatcat
     6361 cgcttcggga ggcatggccg acgcgcgcgg gctcgtcgcg gcgctcgcgc tgggctgcga
     6421 cggcgccaat atgggcacgc gcttcatggc gacggtcgag gcgccgattc acgaaaacgt
     6481 caagaaacag atcgtcgcca atgacgagcg ctcgaccaac ctgattttcc gcacgctgca
     6541 caacaccgcg cgagtcgcga agaatacgat cagcgatgag gtggttgcga tcgagaagcg
     6601 cggcaatgcg acgttcgcgg acgtaaaaga tcttgtcgcg ggaacgcggg gtgccatcgt
     6661 cctcgaccag ggtgagatgg acgccggcat ctggtcggcc ggacagaccc agagcctgat
     6721 ccacgacatt ccgacctgcg ccgaactggt ctcgcgcatc atgcgcgagg cggaagagat
     6781 cattcgccag cggcttggag cgatggtagc acgttgacct gtcgacgccg tcgcttcttt
     6841 ccgctaaaaa cttccggtta tgtaaccgat ataaccttct gatgaacggg ccttagggcg
     6901 agcaaagcgc aactgaaagc gacccgaacg catcgtgggg gacctatggc acgcggcgtc
     6961 gtccgcgttg aaggtcaagc cccaaaaaag gatgccgacc tgattcgggc gctggcccgg
     7021 accctgcgcg gcaaagctga aaacgccaaa gcgcttcgct cgacactgac gaaagccctc
     7081 gttcatcccg aggcgaagaa tgcatttgac gtgttcggat ccgaactgcc cgaagagttt
     7141 tttgccggca tcttcggctg gccgcaccaa cgcagccgac gcaagatagt tttgtgatat
     7201 tcctgctcaa catgattaac cgaacgggcg tgtcgctgct cgatcctttc gccgcatgac
     7261 ggacctgtcg ggacgtccgt tgcttttcgc acaaacagac aggtgagcgg attcaccgcg
     7321 gttcaatgtc tgatcgaacg caggtggttg agcgggcctt ggccgcggcc gaaatccggg
     7381 gccgtctcaa gcgcggcctg gacaaaactg cgcgcggaac ggacggcgtc tgcgagcggt
     7441 cgatgctgcg caagctcaca cgcgattgcg ctcgcgagcg tgcatcccgt gccatgcgtg
     7501 gcgcgcgtcg gaacgcgcgg atattcgaat tcctcgatgc catcccggct gacaaggatg
     7561 tcgcgtatcg gcgtcccgga cgcgtgaccg cctttgatca atacggcgtc cgctcccatc
     7621 cggcgcagcg catctgccgc gtgcagtatg tcgtcacggc ttcccagaac agcgcccgtc
     7681 aggcattccg cctcgggaat attgggcgtc aacagggcag tcagcggcag cagacggtct
     7741 ttcatccgtg tgaccgcatc gccggagaga aacgatgtgc cgctggacga gacgagcacc
     7801 ggatcgagca cgagcggaat gccggaacca tgcgcctcca gagcgtccgc aacggcatcg
     7861 acggcgtccg cggatcccag catcccgatc ttgatcgcat cggcgccgat atcggccaac
     7921 acgcaggcga tctgctcggc aatgatcgaa ggcggaatca agtggatcgc atggacgcgg
     7981 gccgtatcct gcacagtcac cgcggtaacc gccgtcatcg cgtagacgcc caaggcggtg
     8041 gcagtcttga tgtcggcctg gatgccggcg ccgccgcctg aatccgatcc cgcgacgatg
     8101 agcagccgcg caggcgcagc cttcaagcca cgacttcctg cagcgccgca acaacgtcgc
     8161 tcacgaccga gcgcacaagc ctctcgtcgt cggcttccgc catgacacgg ataaccggtt
     8221 ccgtgcccga tttgcgcacc agaagtcggc cggtcccgtt catccgcgcc tcgcaatcct
     8281 tgatgcgctc cacgacgcgg ggatccttca gcggcgagcc gttcttgtag cggacgtttt
     8341 ccaccagctg cggcaggggt tcgaaaatgt gcgccgcttc gctcgcgggc cgtttctcgt
     8401 gcgcgagcac gccaagcacc tgaagggctg cgatcacgcc gtcgccggtc gtacagaaat
     8461 cgcgcagcac gatatgtccg gattgctcgc caccgacatt gaa
//
LOCUS       DASZMC010000113         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_324995,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000113 DASZMC010000000
VERSION     DASZMC010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324995"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="VG274_02225"
     CDS             <1..1878
                     /locus_tag="VG274_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWE05494.1"
                     /translation="PPGETDKVDLWSPLQLDEAKPGGRGQHYLSVLADLRPGVTFTQA
                     QDEMARYAAHSSATVAPTNHPFSPKFHPIVLADFQDEIVKSVRPAMLVLMGAVAFVLL
                     IACVNVANLLLARAEGRRREIAVRAAVGASVRTLVRQFITEGVILSVVGAILGTFLAF
                     AGLRLLAATNAGSIPRANEIAIDWQVLAFTLSLSLLTGIAFGLAPLLHLKLGELAETL
                     KAAGGRNTGDGSTHVFRFVLVSGELALALVLLIGSGLMVKAFWKLQQVDAGVRPDHIL
                     TMRVTLPEPAYKDVAKVRNFWSSLLDGITPLPGVQSASLASGLPPNRRINANDTGIEG
                     YVPAPNGPPANVDYWNFVSPNYFETVGAHLVEGRFLRPSDGFGAPEVVVINQAMERMF
                     WPHESAIGHRIKTGNATTPWRTIVGVMADIKNAGVDRPTGTELFEPYAQSPFTVNTAY
                     LVVRAAGDPTSIAGEVRGQVQKLDRSLPVSSVRSLEDVLNADRARPRFLTMLLTLFSS
                     LSLALAALGIYGVISYAVAQRTNEIGIRMALGAQTRDVLRMISVTGLRLAIAGTAAGA
                     IGAFALTRFMDKLLFGVSSMDVGTFAGMAAILIAVTLLACYVPARRASRVDPLIALRN
                     E"
BASE COUNT          373 a          599 c          756 g          440 t
ORIGIN      
        1 cctcccggcg aaacggataa ggtggatctc tggtcgccgc tgcagttgga tgaggcgaag
       61 ccgggcgggc gtgggcagca ttatctatcg gtgctggcgg acctgcgtcc aggcgtcaca
      121 ttcacgcagg cccaggatga gatggcccgt tacgccgcac acagctcggc gacggtggcg
      181 cctacgaacc accccttcag tccgaaattc caccctattg tgttggccga ttttcaggac
      241 gagattgtga agagcgttcg gccggcgatg ctggtgttga tgggcgccgt ggccttcgtg
      301 ctgttgattg cgtgcgtgaa tgttgccaac ctgctgctgg cgcgcgcgga agggcgcaga
      361 cgcgagattg cggtgcgcgc cgcagtcggg gcgagcgtac gcactctggt gcgccaattc
      421 attaccgaag gcgtgatcct ctcggttgtg ggcgcaatcc tgggcacgtt tctggcattt
      481 gcggggctgc ggctgcttgc cgccaccaac gcgggatcga ttccacgcgc caacgagatc
      541 gcgatcgatt ggcaggtgct tgcgttcacg ctttcgcttt cgcttcttac cggaattgcg
      601 ttcggcctgg ctccgttgct gcacctgaag ctgggcgagc tggcagagac gctgaaggcc
      661 gcgggcggac ggaataccgg cgacggctct acgcacgtct tccgcttcgt tttagtgtcg
      721 ggcgagctgg cgctggcgct agtgttgctg atcggctcgg gattgatggt gaaggcgttc
      781 tggaaactgc agcaggtgga tgcgggcgtg cggccggacc atattcttac aatgcgcgtg
      841 acgctgccgg agccggccta caaggatgtt gcgaaggtcc gcaacttttg gtcatctctt
      901 ctggacggca tcacgccgtt gccgggcgtt cagagcgcgt cgctggcttc gggattgccg
      961 cccaaccgcc gcatcaatgc gaacgataca ggaattgaag gatatgttcc ggcgcctaac
     1021 gggccgccgg cgaatgtcga ctattggaat tttgtaagcc caaattattt cgagaccgtg
     1081 ggggcgcacc tggttgaggg gcgcttctta cgaccgagcg atggcttcgg cgctcctgag
     1141 gtggtggtga tcaatcaagc gatggagcgg atgttctggc cacacgaaag cgcgatcgga
     1201 catcgcataa agacggggaa tgcgacgacg ccgtggcgca cgattgttgg cgtaatggcc
     1261 gacatcaaaa acgcgggtgt ggaccggccg accgggacgg agctcttcga gccgtacgcg
     1321 caatccccgt tcaccgtgaa cacggcgtat ctagtggtgc gggcggcagg cgatccgacg
     1381 tcgattgcag gagaggtgcg cgggcaggtg caaaagctcg atcgctcgct gccggtatcg
     1441 agcgtgcggt cactcgaaga cgtgctcaac gcggaccgcg cgcggccgcg cttcctgacg
     1501 atgctgctga cgctattctc ttcgctgtcg ctggcgctgg cggcgctggg aatttacggc
     1561 gtgatctcct atgcggttgc gcagcgcacg aatgaaatcg gcatccgcat ggcgctgggt
     1621 gcgcagacgc gcgacgtgtt gcgcatgatc tcggtaaccg gactgcgcct ggcgattgcg
     1681 ggaacggcgg ccggggcaat cggggccttc gcgctgacgc gcttcatgga caagctgctg
     1741 tttggcgtga gctcgatgga cgtgggcacc tttgcgggca tggccgcgat tctgatcgca
     1801 gtgacgctgc tggcctgtta cgtgcccgcg cggcgcgcca gccgggtgga cccgctgatc
     1861 gcgttgcgga acgagtaggg gcacgagggc ggcgccctcg atccggtgcg gatgggggcc
     1921 aaaagtcagg agttagaagt caggagttag aagttaggag ctaggagtca gaatgacaaa
     1981 ggctcgcttt cgtgggcttc gctttgaaat gccttcggag ttgcggcagc tgatctagcc
     2041 agcgactcgt tccatgcatt cggagccgca gatggcttct agcgcggttt taaactcttt
     2101 gtcggggcgc actctcgcgg gcacgtccag aagaacggag aagtcgcgcg cggattccag
     2161 acggagac
//
LOCUS       DASZMC010000114         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1966644,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000114 DASZMC010000000
VERSION     DASZMC010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966644"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..377)
                     /gene="trxA"
                     /locus_tag="VG274_02230"
     CDS             complement(24..377)
                     /gene="trxA"
                     /locus_tag="VG274_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HWE05495.1"
                     /translation="MPPLKISDASFQNDVLNASKPVVVDFWAEWCSPCRMIAPALEEL
                     SAELQDKVTVAKLNIDESPATPQKYGVRGVPTLMIFHKGQVAATKVGALPKSKIKEWI
                     EASIDLKSGASVTRA"
     gene            complement(414..>1843)
                     /locus_tag="VG274_02235"
     CDS             complement(414..>1843)
                     /locus_tag="VG274_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWE05496.1"
                     /translation="TLGQPRQSSLWNEIVSRHEEWPRLEFAHTFLRECRARADFTPPY
                     EFFAHVLGPHGGRKRLLGRLGAEANDSIDEFLSLAFAYETLNTPSLEGFLHWLEAGDV
                     EIKRDMERGRDEVRVMTVHGAKGLEADIVILPDTTTIPEGSGRHGVLLYTDNAVVFPV
                     PDAQAPDVVRAAKAKADADALHEHRRLLYVALTRARDELHICGFEGRHGVKEGSWYDI
                     MRPVAEATGIALGNGDDFVRETGDGGPVVRAEASLPRAVLPDWANLPAPEEHARLRLI
                     RPSEGAGEPARKSPLTGGAAMRFSRGLLVHALLAHLPDLPAGERASVARRHLSLRGVD
                     AENQEALLAETFAILDHPQFGAAFAPGSRAEAAIVADLPDLGTGARVSGRIDRLAVTA
                     DAVLAIDFKTNRPAPAHANDVAPLYLRQMALYWAALGKLFPGKRIDCALVWTEGPRLM
                     ALPAELLDGELARIRARLDREGSGS"
BASE COUNT          330 a          587 c          588 g          338 t
ORIGIN      
        1 aggagagaaa gctattcaac cactcaggcg cgggtgacgc ttgctccgct cttcagatcg
       61 atcgacgcct cgatccattc cttgatcttg gatttcggca gcgcgccgac cttggtggcg
      121 gccacctgac ccttatggaa gatcatcagc gtgggcacgc cgcggacgcc atatttctgc
      181 ggcgtggcgg gactctcgtc aatgttgagc ttggcaaccg tcaccttgtc ctgaagttcg
      241 gccgatagtt cttcgagcgc cggcgcgatc atccggcagg gactgcacca ttccgcccag
      301 aaatcgacga caacgggttt gctggcgtta agcacatcgt tctggaaact ggcgtcggaa
      361 atcttgaggg gcggcatgtc gtggtcccag ctggttgcgg atcggcgcta tatctaggaa
      421 ccgctccctt cacggtcaag gcgcgcccgg atccgcgcga gttcgccgtc gagaagttcg
      481 gcaggcagcg ccatcaatct ggggccttct gtccagacga gcgcgcagtc aattcgtttg
      541 cctggaaaca atttgccgag ggcggcccag taaagtgcca tctgccgaag atagagcggc
      601 gccacatcgt tcgcgtgcgc cggcgccgga cggttggtct tgaaatcgat ggcgagcacg
      661 gcgtcagcgg tcacggcgag gcgatcgatt cgtccgctca cgcgcgcgcc cgtgccgaga
      721 tccggcagat cggcaacgat tgccgcctct gcgcgcgagc cgggcgcgaa agctgcgccg
      781 aattgcggat gatcgagaat cgcgaatgtc tccgcaagaa gcgcttcctg attctcggcg
      841 tcgacaccgc gcaatgaaag atggcggcgc gccacgctcg cccgctcgcc tgcgggcagg
      901 tctgggagat gcgccagaag ggcgtgcacg agaagtccgc gcgagaaccg catcgcagcg
      961 cctcccgtca agggcgattt ccgcgccggc tcgccggccc cttcggacgg gcggataagg
     1021 cgtaggcgcg catgttcctc gggtgccgga agatttgccc aatcgggcag cacggcgcgc
     1081 ggaagcgagg cttcggcccg taccaccggt cctccatctc ctgtctcgcg cacgaaatca
     1141 tcaccgttgc ccaacgcaat tcccgtcgcc tcagcaactg gacgcatgat gtcgtaccac
     1201 gagccctcct tcactccatg cctgccctcg aaaccgcaga tatgaagctc gtcgcgcgcg
     1261 cgcgtcagcg cgacatagag gagccggcga tgttcgtgca gcgcgtccgc atcggctttc
     1321 gcctttgccg cgcgcaccac gtcgggcgcc tgggcgtccg gcacggggaa taccacggcg
     1381 ttgtctgtgt agagcagcac gccatgacgg cccgagcctt ccggaatcgt cgtggtgtcg
     1441 ggaagtatga cgatatcggc ctcgagcccc ttggcaccgt gcacggtcat cacgcgcacc
     1501 tcgtcgcgac cgcgttccat gtcgcgcttg atctccacgt cgccggcttc gagccagtgc
     1561 aggaaacctt cgagcgaggg cgtattgagt gtctcgtagg cgaaggcgag cgacaggaat
     1621 tcgtcgatgg aatcatttgc ctccgcaccg aggcgcccaa gcaggcgttt gcggccgccg
     1681 tgcggtccca gcacatgcgc aaagaactcg taagggggcg tgaagtcggc ccgtgcgcgg
     1741 cattcgcgaa ggaacgtgtg ggcaaattcg agccttggcc actcttcgtg gcgcgaaacg
     1801 atctcattcc acagcgacga ttggcgtggc tgcccgagcg tat
//
LOCUS       DASZMC010000115         4936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_327456,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000115 DASZMC010000000
VERSION     DASZMC010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327456"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..852)
                     /locus_tag="VG274_02240"
     CDS             complement(154..852)
                     /locus_tag="VG274_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HWE05497.1"
                     /translation="MVFGQRALTGPRLPAARGSASHLVVLCHGYGADGNDLIGLAPHW
                     QRLLPTVAFAAPNGPERCPGAGYQWFPISRLDKQEVRKGVEAAANALEEFLESELKRL
                     GLPPERLLLAGFSQGTMMSLHVGLRRPHKPIAILGYSGMLAAADSLPVLGPDAPPILL
                     VHGDADTMIPVDAMFAAAGALGRAGGLVQWHLSRGMGHSIDADGLLLGGAFLAMAMRG
                     YLKRPSGQVTCPLG"
     gene            882..1724
                     /gene="gluQRS"
                     /locus_tag="VG274_02245"
     CDS             882..1724
                     /gene="gluQRS"
                     /locus_tag="VG274_02245"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HWE05498.1"
                     /translation="MIVTRFAPSPTGYLHLGHAFAALTAQEAAERGRFLLRIEDLDAT
                     RAREEYVRAILEDLRWLGLEWHGPVLRQSARLDTYRAALDRLAVQELLYPCFCTRAEV
                     AAEIARAAEAPHGPDGPLYPGTCRSLSPNDRAAALGSGTPYALRLDSGRAASRLSGLS
                     FEERGEEGEFRPIAVDPHLFDDIVLARKETPASYHLAVVVDDAEQGVTLVTRGKDLLA
                     ATHAQRVLQTLLGLPAPRYAHHRLILDEAGRKLAKRDGSATLGSLRQQGATAREIRVA
                     LAIP"
     gene            1911..2735
                     /locus_tag="VG274_02250"
     CDS             1911..2735
                     /locus_tag="VG274_02250"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWE05499.1"
                     /translation="MRKAYSCRKMVHAVGFAACAAILALAADEAFAQSSAQPSAQSSW
                     FASTPQSNGQSPSDPRFDEVALRIEANLSDEMIANFNLFIYVDKAETGPLAQRMYVFR
                     KTDQGDLALLYDWSVSTGRERFERDVHGHVQSSFTPKGYFEIDPKRLYADHASAQWDE
                     AMPYAMFFNWKPDGHQTGLAIHGTAEDGESALGTRASAGCVRLSLDNAHTLFDLVRSQ
                     LRGPTPKLAYLDRDSEVSSEGLFLHDAAGRLQSADGYPVLVLVDDYGGENRVTSLY"
     gene            complement(2858..4825)
                     /locus_tag="VG274_02255"
     CDS             complement(2858..4825)
                     /locus_tag="VG274_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HWE05500.1"
                     /translation="MTKRDRPWIFRTYAGHSSAAASNALYRANLAKGQTGLSVAFDLP
                     TQTGYDGDHPLAKGEVGKVGVPVGHLGDMRALFEGLPLAEMNTSMTINAPAAWLLALY
                     VALADEQHVPRAALMGTTQNDIIKEYLARGTYIFPPQPALRLTCDTIAFTTAEMPKWN
                     PVNLCSYHLQEAGADAVEEAAFALAAAIAVLDACRVRGAIPRQEFPNAVGRISFFVNS
                     GVRFITEICKLRAMARIWSEIAKDRYHVEDPKLRLFRYGVQVNSLGLTEQQSENNVYR
                     ILFGMLGVTLSKDARARAVQLPAWNEALGLPRPWDQQWSLRLQQIAAFETDLLEHQDI
                     FDGSHVIESTTAMLAEQIRGEIARIAELGGAASQPALEYMKERLVRSNAARVEAIETG
                     AEIRVGVNAYTESEPSPLTAGEGHFVVVNDAAEREQIARLSDWRAARDADAVASALAA
                     LGSAARSETNIMPSSIECARAGVTTGEWAETLRRIFGEYRAPTGVAAEAPTRNEERIV
                     ALRERVAQASNHLGRKLTFLVAKPGLDGHSNGAEQIAIRARDAGMDVIYDGIRFGADD
                     IVAAASARQPHVVGLSVLSGSHVPLACEIMAKLSEAGLSDVKVVAGGIIPAEDEARLR
                     DAGIAAVFGPKDYDLDAIMREIIGIAVRSKE"
BASE COUNT          887 a         1586 c         1546 g          917 t
ORIGIN      
        1 tgggtaatcg cgcggcgcct aacacggaat cactcctttg aagacccacc tcctctcaca
       61 ggtgggcgaa cattaggggc atgcgcgcac aatgccaacc gccacgcctg cggcgcaatc
      121 tacccgattc ggtgtgaccg aactgcgaca gtctcagccg agcgggcagg taacctggcc
      181 ggacgggcgc ttcaggtatc ctcgcatcgc catcgccagg aacgcccccc cgaggagcag
      241 gccgtctgca tcgatggaat ggcccattcc tcttgaaaga tgccactgaa ccaggccgcc
      301 ggcccggccg agagcgccgg ctgctgcaaa catcgcgtcg accggaatca tggtatcggc
      361 gtcgccgtga acgagcagga tgggcggcgc gtcggggccg agcacaggca gcgaatcggc
      421 ggcggccaac attcccgaat aaccgagaat cgctatgggc ttgtgcggtc gccgaagtcc
      481 tacatgcaac gacatcatcg tgccctggct gaatccggcg agcagcagac gttcgggcgg
      541 caggccgagc cgcttcagct ccgattcgag gaattcctcg agagcattcg ctgcggcctc
      601 gacacccttt cgcacctcct gcttgtccag gcgcgatatt ggaaaccact gatatcccgc
      661 gccgggacag cgctcggggc cattcggcgc cgcgaaggcc acggtcggaa gcagcctttg
      721 ccaatgcggc gccaatccga tcaggtcgtt tccgtccgcg ccgtagccgt ggcagagaac
      781 gacgagatga ctggcgctgc cgcgcgccgc cggcaaccgc gggcccgtga gcgcgcgctg
      841 accgaaaacc aaatccaatc ctccgtctgg agatcggcgc catgattgtc acgcgttttg
      901 cgcccagtcc aacaggctac cttcatttgg gccacgcgtt cgccgcgctc actgcgcagg
      961 aggcggcaga gcgcgggcgg ttcctgctgc ggatagagga tctcgatgcg acgcgcgccc
     1021 gcgaggagta tgtgcgcgca atcttggagg atttgcgctg gctcgggctc gaatggcacg
     1081 gaccggttct ccggcaatcc gcacgtctcg atacctatcg cgcggccctc gatcgcctcg
     1141 cggtgcagga gcttctctac ccgtgtttct gcacccgcgc ggaggtagcg gccgagatcg
     1201 cgcgcgccgc cgaagcaccc cacgggccgg acggtccgct ctatccaggc acatgccggt
     1261 cgctatcccc aaacgaccgt gcagcggcgc tgggttcggg cacgccctac gcgctccgtc
     1321 tcgacagcgg cagagccgcg tcgcggcttt ccgggctttc attcgaggaa agaggagagg
     1381 aaggcgaatt ccggccgatc gccgtcgatc cgcacctgtt cgacgacatt gtcctggctc
     1441 gcaaggagac gccggcatcc taccaccttg cggtggtggt cgatgatgca gagcagggcg
     1501 tcacgctcgt cacacgcggc aaagatctgc tcgccgccac gcatgcgcag cgggtgcttc
     1561 agacactgct cggattgcct gcaccccgct atgcccatca tcgcctcatt ttggacgaag
     1621 caggacggaa gctcgccaag cgcgatggtt cagcgacact cggcagcttg cgtcagcaag
     1681 gcgccacggc gcgcgagatt cgcgtcgctc tcgccattcc gtgatcgcat cggcgactcg
     1741 attcggcccg ggcaatcaac gcttggcgtg aattcgcggc tggggtgtgg ttgcaacttt
     1801 attgcacgta accaaaggcg gtaaacttga agttaccgca cttaggaacc aagtgattgc
     1861 tcgtagcatt atcaatttag tcgttcttgt ctgaccgggg gcagatgccc gtgcgcaaag
     1921 cctattcctg caggaagatg gtgcatgctg tgggtttcgc agcctgcgca gcgattctgg
     1981 cattggcggc agacgaggca ttcgcgcaat cgtccgccca accttccgca caatcttcgt
     2041 ggtttgcatc gacgccacag tcgaatgggc aatcgcccag cgatccgcgg ttcgacgaag
     2101 tcgctctgcg catcgaggcg aatttgtccg acgagatgat cgcgaatttc aatctgttca
     2161 tctatgtcga caaggccgag acggggcccc tcgcgcagcg catgtatgtg ttcaggaaga
     2221 cggaccaggg cgacctcgcg cttctctacg actggtcggt gtccacggga cgcgagcgat
     2281 tcgagcgcga cgtgcatggc catgtgcaat ccagcttcac gccgaaaggc tattttgaga
     2341 tcgatcccaa gcggctgtac gccgaccacg cgtcggcgca gtgggacgag gcaatgccct
     2401 atgcgatgtt cttcaactgg aagcccgatg gccaccagac aggactcgcg atacatggga
     2461 ccgccgaaga cggtgaaagc gcgctcggca cgcgcgccag tgccggatgc gtgcggttgt
     2521 cgctcgacaa tgcgcacaca ctcttcgatc tggtccgctc gcagttgcgg ggaccgacgc
     2581 cgaaactcgc ctacctcgat cgtgactccg aggtgagcag cgaaggtctc tttcttcacg
     2641 acgccgcggg ccgccttcaa tcggccgatg gatatcccgt cctcgtgctg gtggacgact
     2701 acggcggcga gaatcgcgtg acgtcgctgt attgagcgcc acgcagcggc aagtttcgcg
     2761 cttgcagtct ccgggattcc tttccgctca agcgaggcta gaaccgcacc ctcgtttcct
     2821 accccacgac gggggaggaa aattaagtgc gcggtgacta ttctttggaa cgaaccgcga
     2881 tgccaatgat ctcccgcatg attgcgtcga gatcgtagtc cttcggtccg aagacggcgg
     2941 cgataccggc atctctgagc cgggcttcgt cttcggcggg aattatacct cccgccacca
     3001 ctttcacatc ggagagcccc gcctcggaaa gcttggccat gatttcacaa gccagcggca
     3061 catgtgagcc ggaaagcacc gacaagccca cgacatgagg ttgccgcgcg gacgcggcgg
     3121 caacgatatc gtccgcgcca aatcggatgc cgtcatagat cacgtccatg cccgcgtcgc
     3181 gggcgcgaat tgcgatctgc tccgccccgt tggagtggcc gtcgaggccc ggctttgcca
     3241 cgagaaaggt gagtttgcgg ccgaggtggt ttgatgcctg cgcgacacgc tcgcgcaacg
     3301 ccacgatgcg ttcctcgttg cgcgtcggcg cttccgcggc gacgcccgtc ggtgcgcgat
     3361 attcgccgaa tatgcgccgc agcgtttccg cccattcgcc ggtggtgacg ccggctcgcg
     3421 cgcattcgat cgaggacggc atgatgttcg tctcgcttcg cgcagcgctc ccgagcgcag
     3481 cgagagctga cgcgacggcg tcggcatcgc gggccgcgcg ccagtcggac aggcgcgcga
     3541 tctgttcgcg ttcggctgcg tcgttcacga cgacaaaatg tccttccccg gccgtaagcg
     3601 gcgatggttc gctttcggtg tacgcattca cgccgacgcg gatttctgcg cctgtctcga
     3661 tcgcctcgac ccgcgctgca ttcgagcgca cgagacgttc cttcatgtat tcgagcgcgg
     3721 gctgcgaagc cgcgccgccc agttccgcga tgcgggcgat ctcgccgcga atctgctctg
     3781 cgagcattgc cgttgtggat tcgatgacat gcgagccatc gaaaatatcc tgatgctcca
     3841 gcagatcggt ctcgaacgcc gcgatctgct gcagccgcag cgaccattgc tgatcccacg
     3901 ggcgcggcag ccccagcgcc tcgttccagg ccggcagctg aacggcgcgg gcgcgtgcgt
     3961 ccttcgacag cgtaacgccc agcatgccga acagaatgcg atagacgttg ttctcggact
     4021 gctgctcggt gaggccaagc gaattgacct gcacgccgta gcggaagagg cggagcttcg
     4081 gatcctcgac gtggtaacga tccttcgcga tctcgctcca gatgcgcgcc atggcgcgca
     4141 gcttgcagat ctcggtgatg aaccggacgc ccgaattgac gaagaagctg atgcgcccga
     4201 cggcgtttgg aaattcctgc ctggggatcg cgccgcgcac gcggcaggcg tcgagaacgg
     4261 cgatcgcggc cgctagcgcg aacgccgctt cttccaccgc gtccgcgccg gcttcctgca
     4321 ggtgatagga gcagagattg accggattcc atttgggcat ttccgcggtg gtgaaagcaa
     4381 tggtgtcgca ggtcagccgc agcgccggtt gcggcggaaa gatataggtg ccgcgggcga
     4441 gatattcctt gatgatgtcg ttctgcgtcg tgcccatgag cgccgcgcgg ggcacgtgct
     4501 gctcgtcggc gagcgccaca tagagcgcga gcagccacgc cgccggcgcg ttgatcgtca
     4561 tcgacgtgtt catctcggcg agcggcaggc cctcgaacag tgctcgcatg tcgcccagat
     4621 gcccgacggg cacgcccacc ttgccgactt cgcctttggc cagcggatga tcgccgtcat
     4681 atccggtctg ggtcggcaga tcgaacgcga ctgacaggcc ggtctggccc ttggcgagat
     4741 tggcgcgata gagcgcgttc gatgccgcag cgctcgaatg ccccgcatag gtgcggaaaa
     4801 tccacggcct gtcgcgcttc gtcatccctg catcctaata ccagcttttc atgtcccaac
     4861 cgtatttctt aggacatccg tcacaacaaa tgaatcggat cgtgctcggc ggcctgtggt
     4921 gatttgactc gggtcg
//
LOCUS       DASZMC010000116        10360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1433824,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000116 DASZMC010000000
VERSION     DASZMC010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433824"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..733)
                     /locus_tag="VG274_02260"
     CDS             complement(44..733)
                     /locus_tag="VG274_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05501.1"
                     /translation="MVDIFTTVGKSRLARSAKESGTPRAKAGVPRRDGRRKGIANNAN
                     RTGFMWFPPIIVIRFRSVASRKPPAPRHHPLTGAKFGREASLNNTVLANHTILVYHRT
                     YMVQKEGALPGMRAIGHTADAEVHMDAAITLLRQTLPQFGFRIMMPPPLTHAVLPEAC
                     ASIWLRGEAQAQSWHAATPALALLRAIRNELEDQREVRLLRRCTQCKGIGWYIAVNGA
                     KQMCRHACRSV"
     gene            714..2036
                     /locus_tag="VG274_02265"
     CDS             714..2036
                     /locus_tag="VG274_02265"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWE05502.1"
                     /translation="MVNISTTQTLKADAAQAALPDLPPGSPLQDLFKDFQRKNRNLPH
                     HVTALGSGFVIDSSGLIVTNNHVIDGADQIAVTFNDGTVLPATVAGRDEKTDLALVRV
                     NPRSPLPAAHFGDSDRARVGDWVIAIGNPFGLGSSVTAGIVSARNRNIAAGPYDDFIQ
                     TDAPINRGNSGGPLFDMDGNVVGVTSAIFSPSGGSVGVGFAIPSNMARETIAQLRAYG
                     QARRGWIGVRIQQVTSDIADSLGLPSQTGALVADVTAGGPAANGGVLNGDLITRFDGK
                     PVSDSRILPRIVADTPIGKTVDVEVRRKDRKEDLRVTVAKLDETVAAFIPAKVSSLSV
                     PRIVSRLSRLGFAMAPLDSAMREKYRLASDVQGVVITDVDPDSPAAEKNLQPGDVILE
                     VQNETVRTPDDVTRRVDAGAKAGRKAVLLLVSRDGQSNYVGLNLGDAG"
     gene            2050..2961
                     /gene="miaA"
                     /locus_tag="VG274_02270"
     CDS             2050..2961
                     /gene="miaA"
                     /locus_tag="VG274_02270"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWE05503.1"
                     /translation="MTADAVLIAGPTASGKSGLALALAGKIGGALVNADSMQVYREAR
                     ILTARPGEADLLRASHDLYGHVSVQEVYSAARYQSDARAALAKARNRSHIPIFAGGTG
                     LYFSALTEGLAQIPAVPEQVRNGVRERRMRLGAAAFFAEFAARDPETAATLRPGDTQR
                     VLRAAEVLEASGRPILEWQRIAGDAPLGGMKLARFVLSPARSELYRRIDARFEAMIAA
                     GALDEAAGLRGLDPAFPAARMLGLRELWGVLDGVVDLETARSEAKTATRRYAKRQLTW
                     FRHRMADWTWIEAEDADEIFAQAISHL"
     gene            3068..5542
                     /locus_tag="VG274_02275"
     CDS             3068..5542
                     /locus_tag="VG274_02275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWE05504.1"
                     /translation="MPANGLAPVRALPGITPPKSRTPKNKRGKRARKVLEKPARLGWQ
                     TSDEEEVELRRWRGETEIGAIEPLEPEHQHFGCFRVRSGSGGSYDVEIRSFDGFINSC
                     GCIDHRVNGLGTCKHIEGVLAALKKRGPRSFREAALRGSERVEIFLDRQAANKPAIVW
                     PAGNARAFAAAREFLARHVGPNGSKIGDYGHLQALVAAWPGAPAELRRHLRVSTHMAP
                     WLERLRRQRSRLDARMQFLAEVNAGRESFDMLKHPLLPYQRDGMLHLAFGERALLADE
                     MGLGKTIQAIAACELLARRGQASRVLVVCPASLKAEWEEQIARFCERRVRLVFGPRAE
                     RRRAYGERTFFTIVNYEQVLSDADDINAVAAPDVVVLDEAQRIKNWHTKTARCVKSLR
                     SPYAFVLTGTPIENRIDELYSILQYLDPEILGPLFRFNRDFYELDGRGRPVDYKNLGE
                     LRERVSPLILRRRKSDVETQLPARTVKNYFVPMADEQRARYQDYETPASILLALARRR
                     PLTPAEFDRMMMLLACMRMVCDTPAILDPTCRVSPKLEELENILSDLLEEPDRKVIVF
                     SEWERMLQMVRDLAAEMGVECAWHTGSVPQARRRLEINRFKQDPACRLFLSTDSGSVG
                     LNLQVASAVVNVDLPWNPAKLEQRIARAWRKNQMRSVTVANLVCEESIEHNILHLLAS
                     KQAVADGVLDGHGDLSKLKMPSGRGAFLERMQAMMGPRARTLSVEEQIVEDLRTRHGA
                     RALHCEVRTRGEGGEHVLAVLDLDSSALAVEAVRMSKREGTAPPVELISRETWDVLQR
                     LAASGLMQFAGSSPRVLFSTQGGGAG"
     gene            complement(5916..6143)
                     /locus_tag="VG274_02280"
     CDS             complement(5916..6143)
                     /locus_tag="VG274_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05505.1"
                     /translation="MSTNATIKRELGKVATALDENVSDAEMDQLLGDRHDEIEALLEE
                     ARQAIRRGEVAPLEPLHVFLRRARERSKADG"
     gene            complement(6206..7228)
                     /locus_tag="VG274_02285"
     CDS             complement(6206..7228)
                     /locus_tag="VG274_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWE05506.1"
                     /translation="MSTVRTVNCAGFPGTLALLRLSLCSNGTALDWAAAMMPGAASFD
                     GFAPFGAAFMQTMTNLAPEQARLVAMERRGQTMQVKASAAKIVRGGIAWRLVSLANIE
                     TELNAQELTAWQAVIRVLAHEIMNSLTPISSLSSTARDLVAHAQQALPQDNPLSSTLA
                     DARVALDTVARRSDGLVHFVETYRHLSRRLVAKVEPVRLRQVFARIEHLLSGELAARG
                     IILAGSVEPESLEVAADGELLDQALINLLRDAMEALKNRADARIALSASRDSENRIVV
                     AVTDNGPGVPAELRETIFVPFFTTRHDGTGIGLTLTRQIAAVHGAALELCDTDLGGAT
                     FVLRFG"
     gene            7185..8066
                     /locus_tag="VG274_02290"
     CDS             7185..8066
                     /locus_tag="VG274_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWE05507.1"
                     /translation="MPGNPAQFTVRTVDMSPEFPAVYGMKLLAGRNLSRERGADISTT
                     PDRQNDVAAGTNVIIDAMAARMFGFTPPSAVGKVIVIGSHRVTVAGVVQNALFHAARA
                     EPISTLYYFNPIHLGGFSVRANGGQVSQALGVIDSTWRHFAPSVAIRRHFLDDSFDKL
                     FAADEQQGTLFDIFVGIAIFIACLGLFGLAAFSAERRTREIGVRKVFGARTRDVVRLL
                     LWQFSIPVLIANLIAWPIAWYYLDHWLESFAYRITLSLLYFLAAGAAALAIAWATVIG
                     HTLLIARANPVHALRYE"
     gene            8225..8812
                     /locus_tag="VG274_02295"
     CDS             8225..8812
                     /locus_tag="VG274_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05508.1"
                     /translation="MFERTVAVVLLLLVHAALLVLFNPNVTIGTVSPVREITLSLVRS
                     PRARPAPPPIEPTFETPESQPAAPGLAPTSPQPPVTTPMPSQSGLSGLGESLFDCALD
                     NPGNVPADERAHCRHLAAPPAGTAEIGMPKTSKAVQSTRWAAALAARREPPRVPCVSI
                     AQIIEGGGPGAQKSVAIPMVNPLCVLNRLLNSPAK"
     gene            complement(8822..10129)
                     /locus_tag="VG274_02300"
     CDS             complement(8822..10129)
                     /locus_tag="VG274_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HWE05509.1"
                     /translation="MESTSPALLDVHGVCRAFPKPGGGELVVLDGVDLTLNQGEIVGL
                     LGRSGSGKSTLLRLISGLARPNGGSVTYLGHPVEGPARGIAMVFQSFALFPWLTVLEN
                     VELGLEALRLPADDIRTRALAAIDLIGLDGYESAYPRELSGGMRQRVGFARALVVHPN
                     VLLMDEPFSALDVLTAENLRTDLLELWSEGQLPIKGIILVTHNIEEAVLMCDRVLLFS
                     TNPGRIETEISVPLRQPRDRQHPDFAALVDRIYVEMTARRTDRLAPKLDRIPGTGFAM
                     ILPEVPVGLISGLVEAVYAAPYNGRADLPEIASDLQMEIDELFPVVETLQLLRMAEVE
                     GGDIKLTETGKRFANEETDERKRIFARLLIRYVPFASHIRRVLDERDSHTAPKSRFVD
                     ELEDHMTPDMAEQTLRAVVGWARFGELFAYDDDTAMFSMENPA"
BASE COUNT         1884 a         3279 c         3283 g         1914 t
ORIGIN      
        1 gatcaaccga tcgggagcgg tggccatttg cggaagcggt gtcctaaacc gagcggcagg
       61 cgtgccggca catctgtttt gcgccgttga cagcgatgta ccatccgatg cccttgcatt
      121 gcgtgcagcg tcgcagcaat cgcacctccc gctgatcctc aagttcgttc cggattgcgc
      181 gcagcagggc aagcgcaggc gtcgctgcgt gccaagactg cgcttgcgct tcgccacgaa
      241 gccaaatcga cgcacaggcc tcgggcagca cggcatgggt cagcggcggt ggcatcatga
      301 tcctgaatcc gaattggggt agcgtttgac gaagcagcgt gatcgccgca tccatgtgga
      361 cttccgcgtc ggcggtgtgg ccaatggctc tcatccccgg caatgctcct tctttttgaa
      421 ccatgtaggt tctatggtag accaatatgg tatgatttgc aaggacggtg ttgttgaggg
      481 aagcttcgcg tccgaatttt gcaccggtaa gcgggtgatg gcgcggtgcc ggcggtttcc
      541 ggctcgcgac cgatcgaaat cggatgacga taatgggagg gaaccacatg aaaccagttc
      601 ggttggcatt gttggcgatc ccctttctcc tcccgtccct tctggggaca ccggccttcg
      661 ctcggggagt tccggattcc ttcgcggatc tcgccagccg gctcttgccg accgtggtga
      721 atatatcgac cacgcagacg ctcaaggccg acgcggcgca ggccgcgctt ccagatctgc
      781 cgcccggctc accgctgcag gatctgttca aggattttca gcggaagaac aggaatctgc
      841 cgcaccacgt gaccgctctg ggttcgggct ttgtcatcga ttcgtccggg ctgatcgtga
      901 ccaacaatca tgtcatcgac ggagccgacc agatcgccgt gaccttcaat gacggcacgg
      961 tgctgccggc cacggttgcc ggccgggacg aaaaaaccga tctggcactt gtgcgtgtca
     1021 atcccaggtc gcctctgccg gctgcgcatt tcggggactc cgatcgcgcg agggtcggcg
     1081 attgggtgat cgccatcggc aatcctttcg gcctgggaag ctcggtgacc gccggcatcg
     1141 tgtccgcgcg caaccgcaac atcgccgccg gcccctatga cgattttatt cagaccgacg
     1201 cgccgataaa tcgcggaaat tcgggcggcc cgcttttcga catggacggc aacgtcgtcg
     1261 gcgtcacctc ggctatcttt tcgccgagcg gcggttccgt cggcgtgggc ttcgcaattc
     1321 cgtccaacat ggcccgcgaa acgatcgcgc aattgcgcgc gtacggacag gcgcgcagag
     1381 gctggatcgg cgtgcgcatc cagcaggtca caagcgacat cgccgacagc ctcggccttc
     1441 cgtcgcagac cggcgcgctg gtggcggacg tgacggcggg cgggcctgcg gcaaatggcg
     1501 gagtcctgaa tggcgatctc atcacgcgct tcgatggaaa gccggtctcc gattcccgaa
     1561 tcttgccgcg cattgttgcc gatacgccga ttggaaagac agtggatgtc gaagtcaggc
     1621 gcaaggatcg caaggaggat ctgcgcgtca ccgtggcgaa gctcgacgaa accgtcgctg
     1681 catttattcc agcgaaggta tccagccttt ccgtgcccag gattgtatca aggctctccc
     1741 gccttggctt tgcgatggcg cctctcgaca gcgcgatgcg cgagaaatac aggctggcaa
     1801 gcgacgtgca gggcgtagtg atcaccgatg tcgatcccga cagccccgcc gcggaaaaaa
     1861 acctgcagcc gggagacgtg atcctcgaag tgcagaacga gactgttcga acgcccgacg
     1921 acgtgaccag gcgcgtggat gccggcgcca aggcaggccg caaggctgta cttctgctgg
     1981 tgagccgcga cggccagtcg aattacgtcg gattgaatct cggcgacgcg gggtagcgtt
     2041 cccgcaccga tgaccgcgga cgcggtgctc attgcgggtc cgaccgcgag cggcaaatcg
     2101 ggtctggccc tggcgctcgc cgggaaaatc ggcggagcgc tcgtcaacgc cgattcgatg
     2161 caggtctatc gcgaagcgcg gattctcact gcgcgtcccg gcgaggcgga tctcttgcgc
     2221 gcatcgcacg atctctacgg ccatgtcagc gtgcaagagg tgtattccgc ggcgcgatac
     2281 cagagcgatg cacgggccgc actcgcaaag gcgcgcaatc ggagtcacat tccgatcttc
     2341 gccggcggga caggactata tttcagcgcg ctgacggaag gtttggcgca aattccggcg
     2401 gtgcccgaac aggttcgcaa cggagttcgc gagcgccgca tgcggctcgg cgccgccgcg
     2461 ttctttgcgg aatttgcggc gcgcgacccg gagacagccg cgactctgcg cccgggcgac
     2521 acgcaacgcg tattgcgcgc cgccgaagtc ctggaagcga gcgggcgccc gattctggaa
     2581 tggcaacgca tcgcaggcga tgcacccctg ggcggaatga agctcgcccg attcgtgctg
     2641 tcgcccgcgc ggagcgaact gtatcgacgc atcgatgcac gtttcgaagc catgatcgct
     2701 gcgggcgcac ttgacgaagc tgcggggctc cgcggtctcg atcccgcatt tcccgcagcc
     2761 aggatgctgg gactgcgcga gctttggggc gtgctcgatg gtgtggtgga cctcgagact
     2821 gcaaggagcg aggcaaaaac tgcaacgcgc cgctacgcaa aacgtcagct cacctggttc
     2881 cgccaccgca tggcggactg gacatggatc gaggcggaag acgcagacga aatcttcgca
     2941 caagcaattt cacacctgtg atgttatgaa ccggcgacac aagttgtgaa taacctgcga
     3001 gcggcttgcc gcaacgcgcc gcctgcttca gcctttcgct gcgattctca agcaaggtgg
     3061 atttggcatg ccggcaaacg gacttgctcc cgttcgagct ttgcccggga tcaccccgcc
     3121 aaaatcgcgg acgccaaaga acaaacgcgg caagcgcgcg cgcaaagtgc tcgaaaagcc
     3181 ggcgcggctt ggctggcaga cgagtgacga ggaggaagtt gagctcaggc gctggcgcgg
     3241 agaaaccgaa atcggcgcca tcgaacccct ggagccggag caccagcatt tcggctgctt
     3301 ccgcgtgcgt tcgggaagcg gcggctctta cgacgtggaa attcgttcgt ttgacggctt
     3361 catcaattcc tgcggttgca tcgatcaccg cgtcaatggc ctgggcacct gcaagcacat
     3421 cgagggcgtc cttgccgccc tgaaaaaacg cggcccgcgg agcttccgcg aagctgcgct
     3481 gcgcgggagc gaacgtgtcg aaatctttct cgaccggcag gcggcgaaca aacccgcgat
     3541 tgtctggccg gccgggaatg cgagggcgtt cgccgcggcg cgcgagtttc tggcgcggca
     3601 tgtcggtccc aatggcagca agatcggcga ctacggccat ctgcaggcac ttgtcgccgc
     3661 ctggcccggc gcacctgccg agcttcggcg ccatctgcgc gtgtcaacac acatggcgcc
     3721 atggctggag cggctgaggc gtcagcgctc gcgcctcgac gcgcgcatgc aattccttgc
     3781 cgaggtgaat gccggccgcg agagcttcga catgctcaag catccgttgc tgccctatca
     3841 acgcgacggc atgttgcatc tcgccttcgg cgaacgcgcg cttctggccg acgagatggg
     3901 actcggaaag acgatccagg ccatcgccgc ctgcgaactg ctcgcccgtc gcggccaggc
     3961 gtcgcgcgtg ctcgtcgtct gtcccgcttc gctgaaggcg gagtgggaag agcagatcgc
     4021 gcgtttctgc gagcgtcggg tgcggctcgt cttcggtccg cgcgccgagc ggcgccgcgc
     4081 ttatggcgag cgcacgttct tcacgatcgt caattacgag caggtgctga gcgatgccga
     4141 tgacatcaat gcggtcgccg ctcccgacgt cgtcgtgctg gacgaggcgc agcgcatcaa
     4201 gaactggcac accaagacgg cgcgatgcgt gaagtcgctg cgctcgccct acgctttcgt
     4261 gctgaccggg acgccgatcg aaaaccgcat cgacgagctc tactcgattc tgcaatatct
     4321 cgatccggaa attcttggcc cgctcttccg cttcaatcgc gatttctacg agctggacgg
     4381 ccgcggccgg ccggtcgatt acaagaatct cggcgagttg cgagaacgcg tctcgccttt
     4441 gatcctccgg cggcgcaaat ccgacgtcga gacgcagctt cccgcgcgca ccgtgaagaa
     4501 ctatttcgtg ccgatggccg acgagcagcg ggcgcgttac caggattacg aaacacccgc
     4561 ttccatactc ctcgcgctgg cgaggcggcg cccgctcacc ccggccgagt tcgaccggat
     4621 gatgatgctg ctcgcctgca tgcgcatggt ctgcgacacg ccggcgattc tcgatcccac
     4681 ctgccgcgtc agccccaagc tcgaggagct cgagaatatc ctctccgatc tgctcgaaga
     4741 gcccgaccgc aaggtcatcg tcttctcgga atgggaacgg atgctccaga tggtccgcga
     4801 tctcgctgcc gagatgggcg tggaatgcgc ctggcatacg ggaagcgtgc cgcaggcacg
     4861 ccgccgcctc gagatcaacc gcttcaagca ggatcctgcc tgccggctgt tcctctcgac
     4921 cgacagcggc agtgtcgggt tgaacctgca ggttgcaagc gcggtggtga acgtcgatct
     4981 gccgtggaat ccggcgaaac tcgaacagcg cattgcccgc gcctggcgca agaaccagat
     5041 gcgctcggta accgtcgcca atctcgtctg cgaggagagc atcgagcaca acatcctgca
     5101 tctgctcgcc agcaagcagg cggtcgcgga cggcgtgctc gacggccatg gcgacctctc
     5161 gaagctgaag atgccgtcgg gccgcggcgc attcctcgag cgcatgcagg cgatgatggg
     5221 tccgcgcgcg cgaaccttga gcgtagaaga gcagatcgtc gaggacttac gaacgcgtca
     5281 cggcgcacgc gcgctccatt gcgaagtgcg cacgcgaggc gaaggcggcg agcacgtatt
     5341 ggccgttctg gatctcgatt ccagcgcgct cgccgtggaa gcggtgcgca tgtcaaaaag
     5401 ggagggaacg gcgcctccgg tcgaactgat cagccgcgaa acctgggacg tccttcaacg
     5461 cctcgccgca agcggcctca tgcaattcgc cggctcaagc ccgagggtgc tgttcagcac
     5521 gcaagggggt ggcgctggat gaggatcacg aggcgacctc gcctgcaggg ctctcactcg
     5581 ccctcagatt gagggccccc gaatgagacg cgcaaggtcc cgtcgtccgt gcgttatccg
     5641 ccagacaact atgccgtttc cttcctgttg tggttcatag aagatggcgt actcaaagat
     5701 gttaattcgt cgcgggccac cccgatactt ggtccgctga tacatgaggg ggagctcagc
     5761 agcgcggttc attgcctcga atagtcgcgc aatgacaaaa tcggcgatct cagctgatgc
     5821 gctggaagtc agataatccc agatgcggtc gagatcgtcg cttgccggtc gcgacaggat
     5881 gattcgttca ggagtcatgc atcgcgacgg ctgggctagc catctgcctt ggagcgctcc
     5941 cgagctctgc gaagaaatac atgcagaggt tccagcgggg ccacttcgcc gcgccgtatc
     6001 gcctgtcgtg cctcctcaag aagcgcttcg atctcgtcgt gccgatcgcc aagcagctga
     6061 tccatctctg catcggagac gttctcgtcg agggcggtcg cgactttgcc cagttcccgc
     6121 tttatcgtcg cgttggtact catgctgcgc aggatagcac ttctttgcct taccgcaaaa
     6181 gtacgcccaa atcgtgcgtt cgcgcctacc caaatctcag cacgaacgtc gcgccgccga
     6241 ggtcggtatc gcacagctcc agcgcggcgc cgtggacggc cgcgatctgg cgcgtgagcg
     6301 tcaggccgat tccggtgccg tcgtgcctgg tggtgaagaa cggcacgaat atcgtttcgc
     6361 gcagttccgc tggaacgccc gggccattgt cagtgactgc gacgacgatc cggttttcgc
     6421 tgtcgcgcga ggccgaaagc gcgatgcgtg cgtccgcgcg attcttcaat gcctccatcg
     6481 catcgcgcag gagattgatc agcgcctgat cgagcaattc gccgtccgcc gcgacttcga
     6541 gactttcggg ctcgacgctt cccgcaagga tgatgccgcg cgccgccagt tcgcccgaga
     6601 gcagatgctc gatccgcgca aagacctggc ggagccggac cggctcgact ttcgcaacca
     6661 gcctcctgct gagatggcga taggtctcga cgaaatgcac gaggccgtcg ctgcggcgcg
     6721 cgaccgtgtc gagcgccacg cgcgcatccg cgagcgtgga cgaaagcgga ttgtcctgcg
     6781 gcagggcttg ctgtgcatga gccacaagat cccgcgcggt cgatgaaagc gacgaaatcg
     6841 gcgtaagcga attcatgatc tcatgcgcga gaacgcggat gaccgcctgc catgccgtaa
     6901 gctcctgcgc gttgagctcg gtctcgatat ttgccagcga gaccagacgc caggctatgc
     6961 ctccgcgcac gatcttcgcc gccgatgctt tgacttgcat tgtctgtccg cggcgctcca
     7021 tcgccacgag cctcgcctgt tcgggcgcga ggttcgtcat cgtttgcatg aaggcggctc
     7081 caaacggcgc gaaaccgtcg aacgatgccg cccccggcat catcgccgcc gcccaatcca
     7141 gtgccgtgcc gttcgagcag agcgagagcc gcagcaacgc gagcgtgccg ggaaatccgg
     7201 cgcaattcac ggtgcgtacc gtcgacatgt cgccggaatt cccggccgtc tatggcatga
     7261 aactcctggc gggccggaat ctgtcgcgtg agcgcggcgc cgacatcagc accacgcccg
     7321 accggcaaaa tgatgtcgct gccggcacga acgtcatcat cgatgcgatg gccgcgcgca
     7381 tgttcggctt caccccgccc agcgccgtcg gcaaagtcat cgtgatcggt tctcatcgcg
     7441 tgaccgttgc aggcgtcgtc cagaacgcgc tgttccacgc cgcgcgcgcc gaaccgattt
     7501 ccacgcttta ctacttcaac ccaatccatc tcggcggctt ttccgtgcgc gcgaatggcg
     7561 gccaggtttc ccaggccctc ggcgtcatcg acagcacgtg gcgccacttt gctccgagcg
     7621 tcgccatccg ccgccatttc ctcgacgaca gtttcgacaa gctctttgcg gcggacgagc
     7681 agcagggtac gctgttcgat attttcgtcg gcatcgccat cttcatcgcc tgtctcgggc
     7741 tcttcggtct tgccgcattc agcgccgagc ggcgaacgag ggagatcggc gtccgcaagg
     7801 tcttcggcgc acgcacacgc gatgtcgtac ggctgctcct gtggcagttc tcgattccgg
     7861 tgctgatcgc caatctcatc gcgtggccca tagcctggta ctacctggac cattggctgg
     7921 agagcttcgc ctacaggatc accctcagcc tgctctattt cctcgccgcc ggcgcagcag
     7981 cgctcgcgat cgcctgggcg accgtgatcg gtcacacgct cctgatcgct cgcgccaacc
     8041 ccgttcacgc actcaggtat gagtagacag aaactgatgc ggagggtgcg cgaggcggat
     8101 tgcgcgcgga attgatttcc cgctcaattc cggatgcgct gcatgccggg gagatcggaa
     8161 aacggtagat cagcgagtcg cgttcgccgg tgtgccggtc gccaggcgag aacgcccgca
     8221 cagattgttc gaaagaacgg tcgctgtcgt cttgctgctg ctggttcacg cggccctgct
     8281 tgtgctgttc aatccaaatg tcacaatcgg caccgtatcg ccggtccgtg agatcaccct
     8341 ttctctcgtg cgttcgcccc gggcgaggcc cgcgcctccg ccgattgaac cgacgttcga
     8401 aacgccggag agccagcccg ccgcccccgg cctcgcgccg acttctcccc aaccgccggt
     8461 tactacgcct atgccctcgc agtccggtct ctcgggcctg ggagaatccc tgttcgactg
     8521 cgctctcgac aatccgggca atgtgccggc cgatgaacgt gcgcattgcc gtcatctggc
     8581 cgcccctccc gcaggtaccg ccgaaatcgg catgcccaag acgtcgaagg ccgtgcaaag
     8641 cacccgttgg gcggcggccc tggcggcgcg acgcgagccg ccccgcgttc cctgcgtcag
     8701 catcgcgcag atcatcgagg gcggcggccc gggcgcccag aaatccgtgg ccatcccgat
     8761 ggtgaaccca ctctgcgtgc tgaaccggct tctgaacagc cccgcgaaat gaacgtggcc
     8821 tctacgccgg gttttccatg ctgaacatgg cggtgtcgtc gtcataggcg aagagctcgc
     8881 cgaagcgcgc ccagccgacc acggcgcgca acgtctgctc ggccatgtct ggcgtcatgt
     8941 ggtcctcgag ctcgtcgacg aagcgcgatt tgggcgcggt gtggctgtcg cgctcgtcga
     9001 gcaccctgcg aatgtgggag gcgaatggca cgtagcggat gaggagccgc gcgaagatgc
     9061 gcttgcgttc gtcggtctcc tcgttggcga agcgctttcc ggtctcggtg agcttgatgt
     9121 cgccgccttc gacttcggcc atgcgcagga gctgcaatgt ctcgacgacg ggaaacagct
     9181 cgtcgatttc catttgcaga tcggaagcaa tttccggaag gtcggctctg ccgttgtagg
     9241 gcgctgcata taccgcttcg acaagtccgg agataaggcc taccggaact tccggaagga
     9301 tcatcgcgaa tcccgtgcct ggaatgcgat cgagcttcgg cgccagtcgg tcggtccgcc
     9361 tggccgtcat ctccacatag atgcggtcga ccagcgctgc gaaatccgga tgctggcggt
     9421 cgcggggctg gcgcagcggc acgctgattt cagtttcgat gcggccggga ttggtcgaga
     9481 agagaagcac gcggtcgcac atcaagaccg cctcttcgat attgtgcgtc acgagaatga
     9541 tgcctttgat cggcagctga ccttcagacc acaattcaag aagatcggtg cgcagatttt
     9601 cggcggtgag cacgtcgagc gccgagaacg gctcgtccat cagaagcacg ttgggatgga
     9661 cgacgagcgc acgcgcgaac ccgacgcgct ggcgcatgcc gcccgacagc tcgcgcggat
     9721 aggcggattc gtatccgtcg aggccgatga gatcgatggc ggcgagcgcg cgcgtgcgga
     9781 tatcgtcagc aggaagccgc agcgcttcca gtcccaattc cacattctcg agcacggtca
     9841 gccaggggaa aagcgcgaag ctctggaaca ccatggcgat gccgcgtgcc ggcccttcga
     9901 ccggatggcc gagataggtg acactgccgc cattgggccg cgcgaggccg gagatgagcc
     9961 gcagcagcgt cgacttgccg gagccggacc gcccgaggag tcccacaatc tcaccctgat
    10021 tgagcgtgag atcgacgcca tcgagaacga cgagttcgcc gccgcccggc ttcgggaagg
    10081 cgcggcacac gccgtgaacg tcgagaaggg ccggggaggt cgattccatt gagtcacctg
    10141 ttggtgggga gtggcgagtg gcgaatagcg aatagcgagt agcgagtagc gttacggttt
    10201 tttggcttgc agactccgga tcaaggcgcg gagcatgcgg ccgagcgcgc cgcagcgctc
    10261 cagcagggga gcgacagatt cgtcggcagc gattttcacc ctttttgcaa ttatgaggtg
    10321 tgtttccagt tccttgagtg aaccttgcga gaagcgaaga
//
LOCUS       DASZMC010000117         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1434029,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000117 DASZMC010000000
VERSION     DASZMC010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434029"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(731..1708)
                     /locus_tag="VG274_02305"
     CDS             complement(731..1708)
                     /locus_tag="VG274_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWE05510.1"
                     /translation="MKIDERHLAQLAAVVDAGGVTEGAALIGTSQPALSRTLALLEKR
                     IGEPLFVAGRKPLQPTAIGRQLAIHGRAVRAASLKAAETISGFRRGAQGAIRIAGVPF
                     FMDAVVSGFIASFQMKEPDIQIDQSYGHFNELMRRLLGEDIDLAVTPLGTHDISRELV
                     FEPLIAARNVVGCSSNHPLLGKARLTRDDLMRCSWVAPLPGSPLMLDLTNILMTLGVG
                     ELAIRYAGGSLHSVVNYLANSQALAIMPLSVVHSLPAELNISALPLSIPQPERMLGVA
                     YRQSTERDPVNRKFLGHIRSNMSNLSRLVAKRDAAVSWQAGPFMLGSIR"
BASE COUNT          364 a          579 c          568 g          372 t
ORIGIN      
        1 accacgccgg tctcggtttt ccaaagattg acctgttgcg gctttccctt cacgccggcg
       61 cctgagcagt tcagagagca tgcacgtttc ccctggcagt atgttgaccg cgcgaattgg
      121 cggaaaaggt ccgccgcgcg aacccggggc tagtacggcg ctcagagaag agagtttcag
      181 aatttattca cctttttgac aaaaatatta atggacgttt tggatcagtt gaacgatctc
      241 aataaccaag cggggatttg atcgccaaag ggcggcgctt ttggcgggac tcctctgcgg
      301 gctgattgcg agaggctcac aacgatcagc ttgcgcttcg cgtcgaccgg gatctcgacg
      361 ttgggtcttc ggccgacaac ggcatccgat gggttcggct aagctcaatc ctcgcgccac
      421 atgggcccat cccgatcggc cgctccatac gtcgtcggcg aagtccatga atcgcgtccg
      481 ttgatcgcct catccgaagt tcggccgtcg ctgtctggcg cttgcccggt tctcgcaaca
      541 gattttactg cgctgtctcg ccactgatcc gatcgagttc ttgaacgttt agtcgcaact
      601 gctcttcgac ggtgaccagg aggcctttgt ccggcttgcc ggcccggtcg gggtttttct
      661 cagccgcacg ccggcgccgc cgccgttctc aataggtgtc gtaaaaaggg cgtacggcga
      721 catccaaacg ctatctgatc gaaccgagca tgaagggacc ggcctgccac gacaccgcag
      781 cgtcgcgctt ggcgaccaat cggctgaggt tgctcatgtt gctccgtatg tggcccagaa
      841 acttgcgatt gaccgggtcc cgctccgtgg actggcggta ggccacgcca agcatacgct
      901 cgggctgggg aatgctcagc ggcagagcgc tgatgttgag ttccgcaggc aatgaatgaa
      961 ccaccgacaa cggcatgatc gccaacgcct gactgttggc aagataattc accaccgaat
     1021 gcaaggaccc gcccgcgtag cggatcgcca gttcgccgac gccaagcgtc atcaggatgt
     1081 tcgtcagatc gagcatcaac ggtgagccgg gcagtggcgc gacccacgag caacgcatca
     1141 gatcgtcacg ggtcaatcgc gcctttccca acaaggggtg gttcgatgaa cagccgacga
     1201 cgttgcgcgc ggcaatcagc ggttcgaata cgagttctcg gctgatgtca tgcgtcccta
     1261 gcggagtgac ggccagatcg atgtcttcgc ccaacaggcg acgcatcagt tcgttgaagt
     1321 ggccgtagga ttggtcgatt tggatgtccg gctctttcat ctggaacgaa gcgatgaagc
     1381 ccgagacgac ggcgtccatg aagaacggca cgccggcgat ccggatcgcc ccctgcgcgc
     1441 cgcggcgaaa acccgaaatc gtctccgcgg ctttcagcga agccgcccgc acggcgcggc
     1501 cgtggatcgc cagctggcgg ccgatcgccg tgggctgcaa tggcttgcgc cccgccacga
     1561 acagcggctc gccgatccgc ttctccagca gggccaacgt gcgggaaagc gccggctgcg
     1621 acgtgccgat tagcgccgcg ccctcggtca ccccgcctgc gtcgaccacg gcggcaagct
     1681 gcgcaaggtg ccgctcgtcg attttcatag cgtttcgtta taactatctt gtgtcttttt
     1741 atcaacgcgg cgctcagttc gcgctatcgt cgccggcaag ctacggcgga acgccgaagc
     1801 ggcaacggga ggatagcgga tgagcctcat caacggatgg catcacctca ccatgagcac
     1861 cgacggcgct caggaggatt tcg
//
LOCUS       DASZMC010000118         5462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_610594,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000118 DASZMC010000000
VERSION     DASZMC010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610594"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /gene="pgk"
                     /locus_tag="VG274_02310"
     CDS             complement(<1..870)
                     /gene="pgk"
                     /locus_tag="VG274_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWE05511.1"
                     /translation="MAEFRTLDDIAVSGKRVLVRTDLNVPMKNGEVADPLRIERQAPT
                     LRELAAKGARVIVLSHCRRPQGKREPSMSLQPVVPALTAAVGRPIGFADDCVGREAEE
                     AVARLKNGELLLLENTRFHAGEESNDPEFAQAVASLGDLFVNDAFSAAHRAHATTEAI
                     AYLLPSAAGRGMEAELVHLHRALDRPQRPVMAVVGGAKVSTKIAVLAHLLRRVDVLVI
                     GGAMANTFLAAEGLEIGKSLCERDQLAKAREILGTAVAQGVAVVLPVDVVAAKDVKPN
                     AECRVVRAENVLAD"
     gene            complement(870..1877)
                     /gene="gap"
                     /locus_tag="VG274_02315"
     CDS             complement(870..1877)
                     /gene="gap"
                     /locus_tag="VG274_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWE05512.1"
                     /translation="MAIRVAINGFGRIGRLVLRAIVESKRRDIVVVAVNDLGPVETNA
                     HLLRYDSVHGRFPGPVKVDGDHMIVDGHKIRVTACKDPAQLPHTDLGVDIALECTGIF
                     TSKEKASAHLTAGAKKVLISAPADGVDLTVVYGVNHHKLTKTHLVVSNASCTTNCLAP
                     VAKVMHEAFGIAQGFMTTIHSYTNDQPVLDQLHKDLYRARAAAQSMIPTSTGAARAVG
                     LVMPELKGRLDGVAVRVPTPNVSLVDFKFVAKRSTTVDEVNQAAKAAAAGALKGVLDI
                     VEAPLVSSDFNHNPASSSFALDQTKVIDGTFVRVMSWYDNEWGFSNRMADVAVAMGAL
                     L"
     gene            2187..2450
                     /locus_tag="VG274_02320"
     CDS             2187..2450
                     /locus_tag="VG274_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HWE05513.1"
                     /translation="MAELAEAAARLSAALDRLDAVLVPFDEAQQRHVRDSAEIASLTH
                     EREGLLARIAELEDEVRSLASLTEEVEGRLDGAIADIRTALGR"
     gene            2480..2839
                     /gene="zapA"
                     /locus_tag="VG274_02325"
     CDS             2480..2839
                     /gene="zapA"
                     /locus_tag="VG274_02325"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HWE05514.1"
                     /translation="MPLVNVMVNQRAYTIACDEGEEGHLRELAAHVDGKVRGLLESVG
                     QVGEQRLLLMAALLVADDYFDSVGLLERRAQEIEDLAGTRDEMSTRIAGSEEKAALEL
                     EQAAKRVGDIAQRLSRA"
     gene            2853..3010
                     /gene="ssrS"
                     /locus_tag="VG274_02330"
     ncRNA           2853..3010
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VG274_02330"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3707..5011)
                     /locus_tag="VG274_02335"
     CDS             complement(3707..5011)
                     /locus_tag="VG274_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HWE05515.1"
                     /translation="MRRNFASLSKLLICGLVVLPLAAIDGAGAATYTTLHHFKLKNGG
                     LYPAAGLLVDQSGNLYGTTSEGGNGQNCGQSGCGVVFKLAPDGSEAILYSFAGGADGS
                     APETTLIADQTGNLYGTTTGGGGNGCQFGTGCGTIFKLAPDGTETVLHAFQGKNGDGA
                     FPVGDLLLDEVGNLYGTTVQGGNIKCGGTGCGTVFVLAPDGTETALHVFQGGKDGVGP
                     GAGMIADEAGNLYGVTAQGGGSGCSGAGCGIIFELAPDGTETILHAFTGGKHDGAVPE
                     GTLILDSSGNLFGTTIGGGGISPRRCFGFGCGTIFKLAPNETLTVLYAFDGHDGVAPQ
                     GTMIRDQAGNLYGTTYAGGHGYSGRGLDKACYPNGGCGLIFELAADGTETTLHKMSGK
                     SGGRTPSPGLAVDQAGDLYGTTEFGGSKTCFEGCGIVFKISP"
     gene            complement(5133..5354)
                     /locus_tag="VG274_02340"
     CDS             complement(5133..5354)
                     /locus_tag="VG274_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05516.1"
                     /translation="MNFDVDKARSRPHQDGSSAEASRAFAFAFAATGIAMDNKAFDSS
                     EMSKNRVGCTHSGEMHVWNKEQAYVKQIH"
BASE COUNT         1014 a         1710 c         1673 g         1065 t
ORIGIN      
        1 gtctgccagc acgttctctg cccggaccac gcgacattcc gcgttcggct tgacgtcctt
       61 cgcggcgaca acgtccacgg gcaacacgac cgcgacgccc tgcgcgacgg ctgtgccgag
      121 gatttcgcgt gctttggcca actggtcgcg ctcgcaaagc gatttgccga tctcgagtcc
      181 ttccgcggcg agaaacgtat ttgccattgc gccgccgatg accagcacat cgacgcggcg
      241 aagcagatgg gcaagaaccg cgatcttcgt ggagaccttg gcgcctccga cgaccgccat
      301 gaccgggcgt tgcgggcggt ccaatgcgcg gtgcagatga acaagctcgg cctccatgcc
      361 gcggccggcc gcggacggca ggaggtatgc gatcgcctcg gttgtcgcat gagcgcggtg
      421 cgccgcggaa aacgcatcat tgacgaagag gtcgcccaat gacgcaacag cctgcgcaaa
      481 ttcgggatcg ttcgactctt cgcccgcatg gaaacgcgtg ttctcgagca gtagcagctc
      541 gccatttttc aaccgtgcaa ccgcctcctc cgcttcgcgg ccgacgcagt catcggcgaa
      601 gcctattgga cggccgactg ccgcggtcaa ggccggaacg accggctgca gcgacatcga
      661 cggttcgcgc tttccctgcg gcctgcggca atgcgacagc acgatcacgc gcgcgccctt
      721 cgccgccagc tcccgcaacg tcggcgcctg ccgttcgatc cgcagcgggt cggcgacctc
      781 gccgtttttc atcggcacat tgaggtcggt ccgcacgagc acgcgcttgc ccgacacggc
      841 gatgtcgtcg agcgtgcgga attccgccat cagagcaagg cgcccatcgc cacggccacg
      901 tccgccatgc ggttcgagaa gccccactca ttgtcgtacc acgacatcac gcgcacgaag
      961 gtgccgtcga tgaccttggt ctgatccagc gcgaagctcg acgaagcggg attgtggttg
     1021 aaatcggacg acacaagggg cgcctcgacg atatcgagaa cccctttcag cgctccggct
     1081 gcggcagcct tcgccgcctg gttcacttcg tcgacggtcg tggagcgctt cgccacgaac
     1141 ttgaaatcga ccagggagac gttgggcgtc ggcacgcgca ccgccacgcc gtccagccgg
     1201 cccttcagtt ccggcatgac cagtcccacg gccctggcgg ctcccgtcga agtcggaatc
     1261 atcgattgcg ccgcagcgcg tgcgcggtag agatccttgt gaagctgatc gagcaccggc
     1321 tggtcgttgg tgtaactgtg aatggttgtc atgaacccct gcgcgatgcc gaatgcctcg
     1381 tgcatcacct tggccacggg agccagacag ttggtcgtac aggacgcgtt cgaaaccacc
     1441 agatgtgttt tggtaagctt gtggtggttc acgccgtata cgacggtgag gtccacgcca
     1501 tcggctgggg cggagatcag caccttcttc gcgcccgccg tcagatgtgc ggaagccttt
     1561 tccttcgacg tgaagatacc cgtgcattca agcgcgatat cgacaccgag gtctgtgtgg
     1621 gggagttgtg cggggtcttt gcaggcggtg accctgatct tgtggccatc cacaatcatg
     1681 tgatccccgt cgacctttac cgggccgggg aatcggccat gtacggaatc gtagcgcaac
     1741 agatgggcat tggtttcgac cggaccgaga tcgttgacgg ccacgacgac aatgtcgcgc
     1801 cgcttcgatt cgacaattgc gcgcagcacc agacgcccga tgcggccgaa accattgatg
     1861 gcgacgcgga tggccatgat tgtcctccaa tggcgtgcgg ctcagcgccg cgagcgccgc
     1921 gcacgcggat gtttacgccg tgatccggaa atacacatat aggttcttgt ccggcgcctt
     1981 tgcacggatt tccccacatc gacgaaccgg cttgacccgc cgatgtaccg caaccggcat
     2041 cgcggcggtc atggatttcg tgcggaaccc ccagacgcgg ccggccctta gcgaggctgc
     2101 accacgcgcg tcaagtggag gggcggtccg ttgaccccct ttccccgcgc ggcttaaggt
     2161 cgcccgtctt cggcgaaagg attgggatgg cggagctggc agaggccgcg gcgcgtcttt
     2221 cggcggccct cgaccgtctg gatgcggttc tggtcccgtt cgacgaggcg cagcagcgcc
     2281 acgttcgcga ctctgccgaa atcgcttcgc tcacccatga acgcgaaggg ctgctggcac
     2341 ggattgcgga actcgaagac gaggtccgct cgctggcgag cctgaccgag gaagtggaag
     2401 ggcgcctcga cggcgcgatt gccgatatcc ggacggcgct gggccgctga gggcgcttac
     2461 aagtggaggc gaattgcgaa tgccgctcgt caacgtgatg gtcaatcagc gcgcctacac
     2521 gatcgcctgc gacgagggcg aagaaggaca tttgcgcgaa ctggcggcgc atgtggacgg
     2581 caaagtgcgg ggtcttctgg aatctgtcgg gcaggtcggc gagcaaaggc ttctcctcat
     2641 ggccgcactt ctggtcgccg acgattattt cgactcggtc gggcttctgg aacgccgcgc
     2701 gcaggaaatc gaggatctgg cggggacgcg cgacgagatg agtacgcgca tcgcagggag
     2761 cgaagaaaag gcggcacttg agttggagca ggcggcgaag cgggtagggg atattgccca
     2821 gcgactctcc cgggcctagc atggtcggaa gacgggtact gccccgcagg tcaggattgc
     2881 gatacccagg ggccttaatg gtactcaccg ggagctgtcc ctgaccgggg ccgtggcctc
     2941 ggacatatgg cgcccacctg cacttgcagg cccgtgagga tccacatcca acggcggagg
     3001 cggtcccgtc cctcccctgt gctcgcgaat gtgcctgcaa ctccgcgggg gcggcccgcg
     3061 gcacaagctt gcgagcgtca tggcggtttg cgaggcctaa agggtttccg tcgacagtat
     3121 cgtgtcgacg gattcgtctg gttgcaatcg ccgccgacgt tgaagccatt gcttttgata
     3181 gaactcgcag ccacgcactt gccgctgctg gacgctcgtg agatctaggc ccgtatcgcg
     3241 ctaggtgatt ttccgtcgac actataccta ttcacgctgt cgccaacgca tcggttgcga
     3301 cggattttga caaataattt ggggtggcgg gaatggttgc gacttcgcgg cgaccatgct
     3361 cgcgtctaac gttcgccatg accagactgg cgcgaattgt tgtttcgcac gctcccgatc
     3421 acgtcacgca acgcggcaat cgaccggagc cgatcttatt cgaggagggc gatcaggaat
     3481 cgtgcatcga ctagctcgcg gagcaggcac taaaggcgag tgtcgagact tgggcgtatt
     3541 gcttgatgcc aaatcacgtg cacctgatcg ctgcgccggg ggacgaggtt gcgcttgggt
     3601 cgcgctgtcg ccgagacgca tcgccggtac accaatttcg tcaatgcccg cggccgcgcg
     3661 gcggtcagtg ccgaagtcac gcaccggagc cggcacctaa ggaaactcat gggctgatct
     3721 tgaacacaat gccacagcct tcaaagcagg ttttgctgcc accgaattca gttgttccat
     3781 aaagatcgcc ggcctgatcg acggcaagac ccggcgatgg agtacgtcca ccggattttc
     3841 cggacatttt gtggagagtg gtttcggtgc catccgccgc aagctcgaat atgaggccgc
     3901 aaccgccatt cgggtaacac gccttgtcga ggcctctgcc gctataaccg tgcccgccgg
     3961 catacgtggt cccataaaga tttcctgcct ggtcacgaat catggtgccc tggggcgcca
     4021 cgccatcgtg tccgtcaaaa gcatatagaa ctgtcagcgt ttcattcggg gcaagtttga
     4081 agatcgtgcc gcaaccgaag ccgaaacagc gacgaggact gatgccccca cctccgatag
     4141 tcgttccgaa caggttccca gagctgtcga gaattaaagt gccttccgga accgctccat
     4201 catgtttccc ccctgtaaag gcgtgaagaa tcgtctccgt tccgtcggga gccagctcga
     4261 aaatgatacc gcagccggcc ccgctgcagc cacttcctcc gccttgggca gtcacgccgt
     4321 agagattgcc ggcctcatcg gcgatcatgc ccgcgccagg gcccacgccg tccttaccgc
     4381 cctggaagac gtgaagggct gtttccgttc cgtccggcgc gagcacgaaa accgtgccgc
     4441 aaccggttcc gccgcacttg atatttccgc cctgcaccgt tgtgccgtag agattgccca
     4501 cctcgtcgag cagaaggtca cctacgggga aggcaccgtc tccgtttttt ccttggaagg
     4561 cgtgaagcac ggtctcggtt ccgtcgggcg cgagcttgaa aatggtgccg catccagttc
     4621 caaactggca tccgttacca ccgccgccag ttgtcgttcc atagagattt cccgtttgat
     4681 cggcaatcag agtcgtttcg ggcgcagaac catcggcccc gccggcgaag gaataaagaa
     4741 tggcctcgga cccgtcaggc gcgagtttga acacgactcc gcaacctgac tggccgcagt
     4801 tctgaccgtt gccgccctcc gacgtcgttc cgtaaaggtt acctgactga tctaccagca
     4861 ggcccgctgc gggatagagt ccgccgttct tcaacttgaa gtgatgaagc gtggtgtacg
     4921 tcgccgcgcc ggcgccatcg atcgccgcga gcggcaggac gacgagcccg cagatgagca
     4981 acttcgataa gcttgcgaaa tttcggcgca tctctttttc ctccacttcg actgggctgc
     5041 acaacgccac atttctggtg tcagcttgcg caacccgttt cgcctgccca ctccggtcag
     5101 cgtctgccgc gatcacgagg gatgctttgc tgtcagtgaa tctgtttaac atatgcctgc
     5161 tccttgttcc acacatgcat ttcgccgctg tgggtacacc cgaccctgtt tttcgacatt
     5221 tcggacgaat caaacgcttt attgtccatg gcaatgccgg tcgcggcgaa agcgaacgcg
     5281 aacgcgcggc ttgcttcggc gctcgagccg tcttgatgcg gacgtgagcg cgccttatca
     5341 acatcgaaat tcacactcgg ccgctaccga ggaacctgca tgcggattct ttttatcggc
     5401 gacattatcg gacggcccgg ccgcgacgtg gtgtcgaatg aactccccgg tctgcggaac
     5461 gc
//
LOCUS       DASZMC010000119         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2809031,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000119 DASZMC010000000
VERSION     DASZMC010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2809031"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1783)
                     /gene="gpmI"
                     /locus_tag="VG274_02345"
     CDS             complement(203..1783)
                     /gene="gpmI"
                     /locus_tag="VG274_02345"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWE05517.1"
                     /translation="MSRAVKSPSGRPRPAVLCILDGWGSRPDAEDNAITRAEPENYHR
                     MLADCPYALLATSGRAVGLPAAQMGNSEVGHMNIGAGRIVVQDLPRIDDAIADHSFTR
                     RPALLDLIAKAKKAKGTVHVMGLVSPGGVHSHQDHIVALVRAVAGAGLRVRVHAFLDG
                     RDTPPRSAQGFMHLFERAIAGLGDARIATVSGRYYAMDRDKRWDRVEKAYNAIAAGQG
                     RKNRTADEAIAQSYRENIGDEFVAPSTIGGYAGMKDSDALVFSNFRADRAREISTALL
                     DPKFDGFRRERMVKFSAAAGMTEYSRPLTRFMSALFPPEDIASTIGEVVSGLGLRQLR
                     IAETEKYAHVTFFLNGGREETFAGEDRIMVPSPHVATYDRQPEMSAFEVTDRLETAIA
                     SGKYDLIVVNYANPDMVGHTGDQGATEEAVRVIDACLGRLREAVEKAGGVLLVTADHG
                     NAEMMRDPETGEPHTAHTTFDVPIVVVNARRPLKLVEGRLADVAPTLLDLMGIDKPIE
                     MAGHSLARMEAHENVPAS"
     gene            complement(1861..>2749)
                     /locus_tag="VG274_02350"
     CDS             complement(1861..>2749)
                     /locus_tag="VG274_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05518.1"
                     /translation="AAIAAAIACAFSHESAMLCLVLIAALATSRIRNARGFARAAAII
                     AALVTAAGAFYMLRWIVFPRSATERSDFLVSLFGGFLGTPRAPNLSALASLTATAAFL
                     LSQNRRFRVAAAGLGLAVLALMLALLIAMPGDTIAPSRFFAARGLPVALTTVIALVFL
                     LMKPRGLSPAQFANPPVLAVLLGLCVFQPLAQAIITENWNSYVHDLRGVVSTRQGIVT
                     HADAIHALDAQGARFRRELLESWSVEPLSIVLAPHGRVTAVVEPAPLARWTPYRLGDP
                     ATLPRAPGLDWSGFASVQR"
BASE COUNT          501 a          848 c          930 g          470 t
ORIGIN      
        1 cgccaaccgc cgcgaacacg aaaatggcag agaagaaaag acttagcgca gaggacgcgg
       61 aggatgcgca gaggacgcgg cggaagaaga atcgcgcgcc aaaggcgcgc aaccatttcc
      121 tctgcgccca ctgcgcatca cccgcgtcct ctgcgctaaa acgctctttc acttcggccg
      181 taatctccgt gatgaattct tttcaggaag ccgggacatt ttcatgggcc tccatccgcg
      241 cgagcgagtg gcctgccatc tcgatcggtt tgtcgattcc catcagatcg agaagggttg
      301 gcgccacgtc ggcaagccgg ccttcaacaa gcttcagcgg ccgcctggcg ttcacaacga
      361 cgatcggcac gtcgaaggtg gtgtgcgccg tgtgcggctc gccggtttcc ggatcgcgca
      421 tcatttccgc attgccgtga tccgcggtca cgagaaggac gccgccggcc ttctctaccg
      481 cttcgcgcag ccggccgaga caggcgtcga tcacccgcac tgcttcctcc gtcgcgccct
      541 ggtcacccgt gtgtccgacc atgtcggggt tggcatagtt gacgacgatc agatcgtatt
      601 tgcccgacgc gatcgccgtc tcgagcctgt cggtgacttc gaaggccgac atctcgggct
      661 gcctgtcgta ggtggcgaca tgcggactgg gcaccatgat ccggtcctcg cccgcgaagg
      721 tctcctcgcg cccgccattc aggaagaagg tgacatgcgc atatttctcg gtctcggcga
      781 tcctgagttg cctcagcccg agcccggaca cgacttcgcc gatcgtcgag gcgatgtctt
      841 cgggggggaa cagcgcgctc atgaatctgg tcagcggcct ggaatattcg gtcatgccgg
      901 cggcggcaga gaatttgacc attcgttcgc gccggaagcc gtcgaatttg ggatcgagca
      961 gagcggtgga gatttcccgc gcccggtcgg cgcgaaaatt cgagaacacc aatgcgtcgc
     1021 tgtctttcat tccggcatag ccgccgatcg tggagggcgc gacgaactcg tcgccgatat
     1081 tctcgcggta ggactgtgca atcgcctcgt cggctgtgcg gttcttgcgg ccctggcccg
     1141 cagcgatcgc gttgtacgcc ttctccacgc gatcccatct tttgtcgcgg tccatcgcgt
     1201 agtagcggcc ggacacggtg gcgatgcgcg cgtcgccaag gcccgcaatg gcgcgctcga
     1261 acaggtgcat gaagccctgc gcgctcctgg gcggcgtgtc gcgtccgtcg agaaaggcgt
     1321 gaacgcgcac ccgcaagcct gcgccggcaa ccgcgcgcac cagcgccacg atgtgatcct
     1381 gatgggaatg cacgccgccg ggcgaaacga gccccatgac gtgcaccgtc cccttggcct
     1441 tttttgcctt cgcgatgagg tcgagcagcg cgggccgcct ggtgaagctg tggtcggcga
     1501 tcgcatcgtc gatgcgcggc agatcctgca ccacgatgcg gccggcgccg atgttcatat
     1561 ggccgacttc ggaattcccc atctgcgccg cgggcaggcc caccgcacgg cccgatgtgg
     1621 cgagcagtgc ataagggcag tcggcgagca tgcggtgata gttctcgggt tcggcgcgcg
     1681 tgatcgcatt gtcttctgcg tcgggcctcg agccccagcc gtcgaggatg caaagcacgg
     1741 ccgggcgggg tcgcccggac ggagatttga cggctctgct cattcgagaa tcaggcgcgc
     1801 gcgcatggct gcaagatagg aagggcaggc tcgactgtcc acgaatatgg ccgctcgtgt
     1861 tcagcgttga acagacgcaa agccggacca gtccagtccc ggcgctcgcg gcagggtcgc
     1921 aggatcgcca aggcgatagg gagtccagcg tgccaggggc gcgggttcga cgacggccgt
     1981 gacgcggcca tgcggcgcga gcacgatgct gagcggttcg acgctccagg attcgagaag
     2041 ctcgcggcga aagcgcgcgc cttgggcgtc gagcgcgtgt atcgcgtccg cgtgagtgac
     2101 gattccttgc cgcgtgctca cgacaccgcg aagatcgtgc acataggagt tccagttctc
     2161 ggttatgatc gcctgcgcga gcggctggaa tacgcaaagg ccgagaagga ccgcaagaac
     2221 gggcggattg gcgaattgcg ccggggagag tccgcgaggc ttcatcagga gaaagacgag
     2281 cgcaatcaca gtcgtgagcg cgacaggcag gccgcgcgcg gcaaagaagc ggcttggcgc
     2341 tatcgtgtcc ccgggcattg caatgagaag cgcgagcata agcgccagca ccgcaaggcc
     2401 gaggccggct gctgccacgc gaaaccgcct gttttgcgac aacaggaagg cggcggttgc
     2461 ggtgaggctc gcgagcgcgg aaagattggg cgcacgtggc gttcccagga atccgccgaa
     2521 aagactgacg agaaagtcgc tgcgttcggt ggcgctgcgc ggaaagacga tccagcgcag
     2581 catgtagaag gcgccggctg ccgtgacgag tgcggcgatg atcgcggcgg cgcgcgcaaa
     2641 tccccttgcg ttgcggatcc gcgacgttgc gagcgcggcg atcaggacga gacagagcat
     2701 cgcagattcg tgcgagaacg cgcaagcgat cgcggcggcg attgcggcg
//
LOCUS       DASZMC010000120         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4723330,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000120 DASZMC010000000
VERSION     DASZMC010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4723330"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VG274_02355"
     CDS             complement(<1..309)
                     /locus_tag="VG274_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWE05519.1"
                     /translation="MILVDTSVALDVLTDSTWADWSQSALERAAAEEDLAINDVIYSE
                     LSTRYETIEKLNSAVAGLGLHHAAIPRPALFLAGKAFLRYRERGGMRTGVLSDFFIGA
                     H"
     gene            complement(312..533)
                     /locus_tag="VG274_02360"
     CDS             complement(312..533)
                     /locus_tag="VG274_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWE05520.1"
                     /translation="MTTMTSKGQVTVPKRIRDAVGLKPGSRVSFEVRDGNVLLKRESK
                     LPRSRFEKMRGTLKSSLTTDQIMALLRGD"
     gene            623..>1590
                     /locus_tag="VG274_02365"
     CDS             623..>1590
                     /locus_tag="VG274_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05521.1"
                     /translation="MTPAARLQSAIEVLDALQHTAKPADRFLRDWFRARRYAGSKDRA
                     AVAERVFTVLRRRASLAWRMQDEQPRSLVLASLVAESGDLATIDGLFNGAGYGPPPLS
                     ESERAAIHSPLADDPPLWVQGEFPQFLEPELARSLGGALLDEMRAMCGRAPIDLRTNT
                     VKATRDDVLRELRGEGFAAEPTPYAPHGVRIEKHEGAAALGRTRAFLDGRFEFQDEAA
                     QIASLLCAASPGEHILDLAAGAGGKALALAAAMNNEGQILARDNDAGRLSQLAPRAAR
                     AGVTIIREELAQADPPGASFDAVFVDAPCSGSGAWRRNPDAKWRMRP"
BASE COUNT          269 a          532 c          510 g          279 t
ORIGIN      
        1 gtgagcgccg atgaaaaaat cggaaagcac gccggttctc atgccgcctc gctcccggta
       61 gcgcaaaaac gccttcccgg cgagaaagag agcaggtctt gggatcgcag catgatgcag
      121 gcccagtccg gcaacggcgg aattcagctt ttcgattgtc tcgtaccgcg tcgatagttc
      181 cgagtagatg acatcgttga ttgcaagatc ctcttcggct gccgcacgct cgagggccga
      241 ttgcgaccaa tcggcccagg tggaatcggt cagcacgtcg agtgcaacgc tggtatcgac
      301 caggatcacg ctcagtcgcc ccgcaggaga gccatgatct ggtccgtggt cagcgacgat
      361 ttcagcgttc cccgcatttt ttcaaatcgg ctcctgggaa gcttcgactc gcgcttgagc
      421 aggacgttcc cgtcccgcac ttcgaagctg accctgctgc ccggtttcag tccgacggca
      481 tcccgtatac gcttgggcac ggtcacctgg cccttgctgg tcattgtggt catggcggcg
      541 ctcgtggtaa tactgatttc gaagagtagt attaccgatt gtcagggacc ctgcaagggg
      601 tccggattgc actgatcccg acatgactcc cgccgccaga ctgcaatccg ccatcgaagt
      661 cctggacgcg cttcagcaca cggcgaagcc ggcggaccgg tttctgcgcg actggtttcg
      721 ggcgcggcgc tatgcgggat cgaaggaccg tgcggccgtc gccgagcgcg tgttcacggt
      781 gctgcgccgg cgcgcctcgc tggcctggcg catgcaggac gagcagccgc gttcgctcgt
      841 gctggcgagc ctcgttgcgg aaagcggtga tctcgcaacc atcgacgggc tcttcaatgg
      901 tgccggttac gggccgccgc cgctcagcga atccgaacgt gcggcgatcc attcgccgct
      961 tgccgatgac ccgcccttgt gggtgcaagg cgaatttccg caattcctcg agccggaact
     1021 cgcccgcagc ctgggcggcg cgcttctcga cgagatgcgc gccatgtgcg ggcgcgcgcc
     1081 gatcgatctg cgcacgaaca ccgtcaaagc cacgcgcgac gatgtgcttc gcgagttgcg
     1141 cggcgagggg ttcgccgccg agcccacgcc ctacgcgccg cacggcgtcc gcatcgaaaa
     1201 gcacgaaggc gcggcggcgc tgggccgcac gcgcgccttc ctcgacggcc gcttcgaatt
     1261 ccaggatgaa gcggcacaga tcgcctctct cctctgtgca gcgagcccgg gcgaacacat
     1321 tctcgatctc gcagccggcg ccggcggcaa ggcgctcgct ctcgctgcgg cgatgaacaa
     1381 tgagggccaa atcctggcgc gcgacaatga tgccggccgg ctgtcgcaac tcgcgccgcg
     1441 cgccgcgcgg gcgggcgtca cgatcatccg cgaagaactc gcgcaagccg atccgccagg
     1501 cgcgtcgttc gacgccgtat tcgtcgatgc gccctgcagc ggctccggcg cgtggcggcg
     1561 caaccccgac gccaaatggc ggatgcgccc
//
LOCUS       DASZMC010000121         9843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5000869,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000121 DASZMC010000000
VERSION     DASZMC010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5000869"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..796
                     /locus_tag="VG274_02370"
     CDS             269..796
                     /locus_tag="VG274_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWE05522.1"
                     /translation="MNAHWFPFHPKIKTASEETRMTGRIDSRLKGLGIVLPAPPAPVA
                     SYVPYVTVDNLVFISGQVTLAADGLHFVGIVGKDLSVEQGHAAARLCAINVVAQLKAA
                     LGGDLDRVKRCVKLAVFVNAVPGFTQHPEVANGASDLIVEVFGELGRHARAAVGAGSL
                     PRNVATEVEAIFEIA"
     gene            793..1959
                     /locus_tag="VG274_02375"
     CDS             793..1959
                     /locus_tag="VG274_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE05523.1"
                     /translation="MTAEFVITRIARSAAEIGAAAWNACADPDGSADRHPFTRFEFFA
                     ALESSRSASPETGWNPCHLVLETGGSIKAILPLYLKNHSLGEYVFDQGWAEALERAGG
                     HYYPKLQASVPFTPVTGPRLLVAGATQRTQVQKALLLAGVDAANRLKASSLHVTFMTE
                     AESSAAAACGFLLRTDQQFHWENEAYSSFEDFLGRLSSSRRKNLRKERAAVREEGIAF
                     EWLVGNEIREAHWDAFFRFYMTTGSRKWGRPYLTREFFSRIGAEMGESVILILAHRGG
                     HPIAGALNFIGERVLFGRNWGAGEFVPFLHFETCYYQAIEFAIANRLRKVEAGAQGPH
                     KLLRGYRPVATHSAHYIAHDGLRSAVARYLSHERELVASEIAEAAQVAPFRKEE"
     gene            1956..2381
                     /locus_tag="VG274_02380"
     CDS             1956..2381
                     /locus_tag="VG274_02380"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HWE05524.1"
                     /translation="MTSMPYDSNNVFAKILRGELPSVKIFEDSHTLAFMDLMPSAEGH
                     TLVIPKESAETIFDLSRTGAAALMATTQIVADAVRKALNCEGLMLVQLNGATAGQSIA
                     HVHFHIGPRSEGLDLRLHGRSKADPQVLEAIAARIRAQL"
     gene            complement(2402..4735)
                     /gene="clpA"
                     /locus_tag="VG274_02385"
     CDS             complement(2402..4735)
                     /gene="clpA"
                     /locus_tag="VG274_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HWE05525.1"
                     /translation="MPSLSRSLEQVLHRAIKLASDRHHEYATLEHLLLALLDDSDAAQ
                     VMKACNVELDALRRSLLKYVDHELLTLVIEDGEDAKPTTGFQRVVQRAVLHVQNSGRD
                     EVTGANVLVALFTERESHAVYFLQEQNMTRLDAVSYISHGIAKRPGMTQQKSARGADE
                     DEDGEKPAKQGNDALEAYCVNLNEKAKAGRIDPLIGRDAEVERTIQILCRRQKNNPLF
                     VGDPGVGKTAIAEGLARKIVKHDVPEVLQTSTIYALDMGALIAGTRYRGDFEERLKSV
                     VKELEAIAGAILFIDEIHTVIGAGATSGGAMDASNLLKPALQAGTLRCIGSTTYKEYR
                     QYFEKDRALVRRFQKIDVTEPTVPDTIKILKGLKPYFEEYHKVRYTAEAIKASVELAA
                     KYINDRKLPDKAIDVIDEVGASQMLLPESRRKKVIGVKEVEEVVAKMARIPPKSVSKT
                     DTEALRNLETELRRVVYGQDPAIHALSSAIKLARAGLRHPEKPIGCYLFSGPTGVGKT
                     EVARQLAGAMGVELLRFDMSEYMERHTISRLIGAPPGYVGFDQGGLLTDGVDQHPHCV
                     LLLDEIEKAHPDLFNILLQVMDHGKLTDHNGKRIDFRNVVLIMTTNAGAAESAREAIG
                     FGRVRREGDDEEAIKRLFTPEFRNRLDATIAFAPLSRDTIDRVVEKFVLELEAQLSDR
                     DVSFDLTPEATRWLGQKGYDDHFGARPLARVIQDHIKKPLADEILFGRLKGGGTVRVL
                     LDRETDQLAFEFIANESGGPKALPPPGGGEPNRTETV"
     gene            complement(4743..5078)
                     /gene="clpS"
                     /locus_tag="VG274_02390"
     CDS             complement(4743..5078)
                     /gene="clpS"
                     /locus_tag="VG274_02390"
                     /inference="COORDINATES: protein motif:HMM:NF000669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWE05526.1"
                     /translation="MSSKNPRGTSGPNGPATGVATKTRPKTKRPSLYKVLLLNDDYTP
                     MEFVVHILENFFNKARDEAVQIMLHVHRHGVGICGIFTYEVAETKVAQVIEFSRRHQH
                     PLQCTMEKE"
     gene            complement(5263..5724)
                     /gene="phaP"
                     /locus_tag="VG274_02395"
     CDS             complement(5263..5724)
                     /gene="phaP"
                     /locus_tag="VG274_02395"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HWE05527.1"
                     /translation="MANNRTKAGDKAVGAEAIETAMKNGSEAFKTGFEKAVRSYDNLF
                     GYSRETVEAYVKAANAAGKGVETLHNELYTYSRQSVEDSISATRALFASKSVHEALEL
                     QTDFVKSSFDAYVGQVTKLSEIMFSATKEAISPLQGRVQAWAEIVESARAA"
     gene            6049..7737
                     /locus_tag="VG274_02400"
     CDS             6049..7737
                     /locus_tag="VG274_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWE05528.1"
                     /translation="MALLLGGVLAGTLCCGSAGASIRRHHRPFARTFLTLTDPGKDAA
                     LVVDGETGRPLYARNADALRHPASLTKMMTLYLLFEQLKSGQMTLATPLSVSEHAAVQ
                     ARTKLNLRPGTSLPVDTAIKAIVVLSANDVAVTIAEAIGGTEGHFAELMTAKARALGM
                     THTYYDNASGLPDPLQITSASDLVILARHLAYDFPQYFHYFSTYSFGYRGATYFTHDN
                     LIGRYEGADGIKTGYTGASGFNLVSSVVRNGVHIIGVVMGGVTARRRDSEMVRLLDDT
                     FASIAKNPRLAARETIPWTAMASNTETRPVIAGFTLGNAGNTLPPALPALPANRSLLP
                     GPSAVSADDEDAAESRPDAAYESLKNPPEAVVTSAPVSNHPAVANHPGPAASPVGSAP
                     AAISLPVAQAPMSLAVTPPHSEAKAATATELRANTPRPQPKPSMELADYQPPALEPLV
                     LHPRDPARRDSAPPEIPVSRPLSAALGKADWTIQIGAFDDAADAKAELAAYAGRSTDV
                     LGQAARIVAPIQSAEGHVLYRARFGPFIEHQAREVCQLLTVRGETCFAALSVRQ"
     gene            complement(7778..8251)
                     /locus_tag="VG274_02405"
     CDS             complement(7778..8251)
                     /locus_tag="VG274_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWE05529.1"
                     /translation="MIAVLAGGVALVAILMLLRAFAGADTRLVTRGVRYSGAAVLLCA
                     AVGLAAIDRVGLAMLAGSFAWGLFTGGRAWPDGWPFSGGRSTSSEEPTARAGSAMPRA
                     EALKLLGLEPGATRDEINAAHRRLILQIHPDRGGTSYLAARINEAREVLLNGRGP"
     gene            complement(8260..8478)
                     /locus_tag="VG274_02410"
     CDS             complement(8260..8478)
                     /locus_tag="VG274_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05530.1"
                     /translation="MSRSLKALALVAIVAAGPAGAIAGAAAAERGKFVELAACLGVIA
                     VSIPALVIIRAERRRRAMPLLPSRDAPM"
     gene            8735..9307
                     /locus_tag="VG274_02415"
     CDS             8735..9307
                     /locus_tag="VG274_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05531.1"
                     /translation="MNTTISPALAIVLAAIVIIVLLATIAFIWWRERRSKQLRSRFGQ
                     EYLRTLEEIGSRHEAEARLHRLEKRVESFSIRALPPEEKARYAASWRKVQADFVDSPG
                     EALNRADELLGSVMKARGYPVSDFEQRSADLSVDHAAVVQNYRAAHDIALRHKRGEAD
                     TEALRQAMIHYRALFAELVDETDEARAKAS"
     gene            9393..9824
                     /locus_tag="VG274_02420"
     CDS             9393..9824
                     /locus_tag="VG274_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05532.1"
                     /translation="MNEALQVDKEYGEERHKELATANLAGTKPLDVAWRESRERENSG
                     AANWSGDDEKAAPLLLAEDADRMRAEWDSIQAGFVDEPRAAVEKADNLVAATMKRLTE
                     NFAEERSKLESQWDRGDDVSTEDLRIALRRYRSFFQRLLSV"
BASE COUNT         1759 a         3153 c         3012 g         1919 t
ORIGIN      
        1 caaaactgga aactgcccgc gcgacgcctg tcgtgacttt ttttgcccgc gcgaggccgt
       61 ccgcgctgcc ccacgcttcc gttcggcaag gccagcacat ccatgtgcgg cgatgcgctg
      121 cccattccgc cggcatccaa aggcccccgg atgaggcttt cgtcacagga gatccgttcg
      181 tcatgacgct tacataacca ttcgggaccg ggacagggag gccgagatgg tctgcgccgc
      241 ctcgccaggg cttgctggcg cgcgcgatat gaatgcacat tggttcccgt tccatcccaa
      301 gatcaagacc gcctccgagg agactcgcat gaccggacga atcgacagcc gattgaaagg
      361 gctcggcatc gttttgcctg caccgccggc gccggtggcg agctatgttc cctacgtgac
      421 cgtggacaat ctggttttca tttccggcca ggtgacgctg gcggccgacg gactccattt
      481 cgtcggcatc gtcggaaagg acctgtcggt cgagcagggc catgccgccg cgaggctgtg
      541 cgcgatcaat gtggtggcgc agctcaaggc ggcgctgggc ggagacctcg atcgcgtgaa
      601 gcgttgcgtg aagcttgccg tattcgtaaa tgcggttccc ggcttcactc agcatcccga
      661 ggtcgccaac ggcgcctccg atctgatcgt cgaggttttc ggcgagttgg gtcggcacgc
      721 gcgcgccgcg gtcggcgcgg ggtcgctgcc gcgcaacgtg gcgacggaag tcgaggctat
      781 tttcgagatc gcgtgaccgc agagttcgtc ataacgcgca ttgcacgctc tgcggcggaa
      841 attggcgctg cggcatggaa cgcatgcgca gaccccgacg gttcggccga taggcacccg
      901 ttcacgcgtt ttgaattctt tgccgccctc gaatcctcgc gctccgcatc gccggaaacc
      961 ggctggaatc cctgtcatct ggtgctcgaa accggcggtt cgatcaaggc cattcttccg
     1021 ctttatctga agaaccacag cctcggcgaa tatgtcttcg accaggggtg ggccgaggcg
     1081 ctggagcgcg ccggcggcca ctattatccc aaactccagg cctcggtgcc gttcacgccc
     1141 gtgaccggcc cccggctgct cgtcgccggg gccacgcagc gaactcaggt ccagaaggca
     1201 cttctgttgg cgggagtgga cgcagcgaac cggctgaaag cctcctccct ccacgtcacc
     1261 tttatgacgg aagcggaatc gtctgcggct gccgcgtgcg gctttctgct gcgcaccgat
     1321 cagcagttcc attgggaaaa cgaggcctat tcgtcattcg aggattttct cggccgcctg
     1381 tcttcttcga ggcgcaagaa tcttcgcaag gagcgcgctg cggtgcgcga ggaaggcatc
     1441 gcgttcgaat ggctcgtggg caacgaaata agggaagcgc attgggatgc tttttttcgg
     1501 ttctacatga caacgggaag ccggaaatgg gggcgcccct acctgacccg cgaattcttc
     1561 agccggattg gcgctgaaat gggagaatcc gtgatcctga tactggcgca tcgcggcgga
     1621 catccgatcg ccggggcgtt gaatttcatc ggcgagcgcg tccttttcgg gcgcaactgg
     1681 ggcgccggcg aattcgtccc cttcctgcac ttcgagacct gctactacca ggcgatcgag
     1741 tttgcgatcg ccaaccgctt gcgcaaagtg gaggcgggcg cccagggccc gcacaagctg
     1801 ctgcggggtt accggccggt cgccacgcat agcgcccatt acattgccca cgacggattg
     1861 cgcagcgccg tggcacgcta cctttcgcac gaacgggaat tggtggcttc ggaaatcgcc
     1921 gaggccgcac aggtggcgcc cttcaggaaa gaggaatgac ttcgatgcct tacgattcaa
     1981 acaacgtatt tgcaaaaatc ctgcgcggcg aattgccgag cgtgaaaata ttcgaggaca
     2041 gccacacgct cgccttcatg gacctcatgc cctccgccga ggggcacacg cttgtcattc
     2101 caaaggagtc cgcggaaacg attttcgatt tgtcgcgaac gggcgccgcg gccctcatgg
     2161 caacgaccca aattgtggcc gacgccgtgc ggaaagcatt gaattgcgag gggctcatgc
     2221 tggttcagct gaatggcgcg actgcgggcc agagcatcgc acatgtccat tttcatatcg
     2281 ggccgcgctc ggaggggctc gatctcaggc tgcacggccg cagcaaagcc gatccgcagg
     2341 ttctggaagc aatcgccgcg cggatccgcg cacagctctg accggccggc agctcgcgcg
     2401 gctatacggt ttccgtccga ttcggctcgc ctcccccggg cgggggaagc gccttgggtc
     2461 ctccggattc gttggcgatg aactcgaatg ccaattggtc cgtttcgcgg tcgagcagca
     2521 ctctcacggt gcctccgccc ttcagccgtc caaacagaat ctcgtcggcg agcggcttct
     2581 tgatgtgatc ctggatgacg cgcgccagcg ggcgcgcgcc aaaatggtcg tcatagcctt
     2641 tctggccgag ccaccgcgtc gcctccggcg tgagatcgaa gctgacatcg cggtcggaga
     2701 gctgcgcctc cagctcgagc acaaacttct cgacgacgcg gtcgatcgta tcgcgcgaca
     2761 gcggtgcgaa ggcgatcgtg gcgtcgaggc ggttgcggaa ctccggcgtg aacaggcgct
     2821 tgatggcttc ttcgtcgtcg ccttcgcgcc tgaccctgcc aaatccgatc gcttcccgcg
     2881 cggattcggc cgccccggca ttcgtcgtca tgatcagcac gacattgcgg aagtcgatcc
     2941 tcttgccgtt atggtccgtc agcttgccgt gatccatgac ctgcaagagg atgttgaaca
     3001 gatcgggatg cgccttttcg atttcgtcca gcagcaatac gcaatgggga tgctggtcga
     3061 ctccgtcagt cagcagtccg ccctgatcga agccgacata gcccggtggc gcgccgatca
     3121 gccgggagat cgtatggcgc tccatgtatt ccgacatgtc gaagcgaagc agttccaccc
     3181 ccattgcgcc ggcgagctgg cgcgcgacct ccgtctttcc cactccggtc gggcccgaaa
     3241 acaggtagca gcctatgggc ttctccggat gccgcaggcc ggcccgcgca agcttgatcg
     3301 ccgaggacag cgcgtggatc gcggggtcct gcccgtaaac aacccggcgc agttccgtct
     3361 cgagattgcg cagcgcttcc gtatcggtct tcgacacgga tttgggagga atgcgcgcca
     3421 tcttcgccac gacttcctcg acttccttca caccaatgac cttcttgcgg cgcgattcgg
     3481 gaaggagcat ctgcgaggcg cccacttcgt cgatgacgtc gatcgccttg tcgggcagct
     3541 tgcggtcgtt gatgtatttg gcggccagct ccactgacgc cttgatggcc tccgccgtat
     3601 agcggacctt gtgatattcc tcgaaatagg gcttcaggcc tttcaggatc ttgatcgtgt
     3661 cggggaccgt gggttccgtc acatcgatct tctggaagcg gcgcaccagc gcccggtcct
     3721 tttcgaaata ctggcgatat tccttgtagg tggtggagcc gatgcagcgc agcgttcctg
     3781 cctgcagcgc gggcttgagg aggttcgatg catccatggc gccgccgctt gtcgcgcctg
     3841 ccccgatgac cgtgtgaatt tcgtcgatga agaggatggc gccggcaatg gcctcgagct
     3901 ccttgacgac ggacttcagc cgttcctcga aatctccgcg ataacgcgtt ccggcgatca
     3961 gcgcgcccat gtccagcgcg tagatggtac ttgtctggag cacttccggc acatcgtgct
     4021 tgacgatttt gcgcgccagg ccctcggcga tcgccgtctt gcccactccc ggatcgccga
     4081 cgaagagggg gttgttcttc tggcgccggc aaagaatctg gatcgtccgc tcgacttccg
     4141 catcgcgccc gatcagggga tcgatgcggc ctgccttggc tttctcgttg agattgacgc
     4201 aataggcttc cagggcgtcg ttgccctgct tcgccggttt ttcgccgtct tcgtcctcgt
     4261 cggcgccccg cgccgacttc tgctgggtca tgcctggacg ctttgcgatg ccgtgactga
     4321 tgtagctcac cgcatcgagg cgggtcatgt tctgttcctg gagaaaataa accgcgtgac
     4381 tttcgcgctc cgtaaagaga gcgacgagca cgttggcccc ggtcacctcg tcgcgcccgg
     4441 aattctgaac gtgcaggacg gcgcgctgca cgacgcgctg aaagcccgtt gtcggtttgg
     4501 cgtcctcgcc atcctcgata accagcgtca acagttcgtg atcgacatat ttcagcagcg
     4561 agcgccgcag cgcgtcgagt tcgacgttgc aggccttcat gacctgggcc gcgtcggaat
     4621 cgtcgagcaa agccagcaaa agatgttcga gtgtcgcgta ctcgtggtgg cgatcgctcg
     4681 cgagcttgat agcgcgatga aggacctgct cgaggctccg cgacagcgag ggcatttacg
     4741 tcctactcct tttccatcgt acactgcaga ggatgttgat gccggcgtga aaattcaatc
     4801 acctgcgcca cttttgtctc cgcaacctcg taggtaaaga tgccacagat tcccaccccg
     4861 tggcgatgca cgtgcaacat gatctgaacc gcttcgtccc tcgccttgtt gaagaagttc
     4921 tcgagtatat gaaccacaaa ttccatcggc gtgtaatcgt cgttgagcaa cagaaccttg
     4981 tacaggctcg gccgtttcgt cttcgggcgc gtttttgtcg cgacgccggt ggccggtccg
     5041 ttcggtccgg aggttccgcg cgggttcttg ctgctcatat ccgaaactga aggcggtctc
     5101 ccgccgatga ttgcgcattc gaagcttaat cgaaagaccc cgcgggcgcc caatgcggca
     5161 tgcgcaaaag aggaaaggcc cgaaccttgc ggcccgggcc ctttgcacgc gctccgcggg
     5221 ggaatgcgga acacagttgc gctcgatttg tttacgcgcg gttcatgcag cgcgggcgct
     5281 ctccacgatc tccgcccagg cctgcacgcg gccctgcagc ggactgatcg cttcttttgt
     5341 cgccgaaaac atgatctcgc tgagtttcgt cacctgtccg acataggcgt cgaacgagga
     5401 ctttacaaag tcggtctgca gctcgagggc ttcatgaacc gatttgctgg cgaacagcgc
     5461 cctggtggcg gaaatcgaat cttccaccga ttgcctggaa taggtgtaga gctcgttgtg
     5521 gagcgtctcc acgcccttgc ccgcggcatt cgccgccttg acataggctt cgacggtttc
     5581 gcggctatag ccgaaaaggt tgtcgtagct ccgaaccgcc ttctcgaaac ccgtcttgaa
     5641 cgcttccgaa ccgtttttca tcgcggtttc gatcgcttcg gcgccgacgg ccttgtcgcc
     5701 ggccttcgtc ctgttgtttg ccatggtggc tctcctcgta acgttttcgt ggggtgacca
     5761 gcgttatgct gcaatgcagc atgccgtata tgcagtgcag cattcgcctt gtcaagagga
     5821 ttgagcccag tggccggcct ccaagcccgg aaccacggca tccgtcggcc gttcaccact
     5881 taacttatcc taaataccgg gaatctaagg tggattctcg aaatcgattg caactcggcg
     5941 gctgatttgc tcttgtgccg gtcgacagga ttcgcggcac agtttggagt tcaaatgttg
     6001 gaacgatcgc gcgccgttct tcgcgcgttc aggcaccagc gtgcgtgcat ggcgctcttg
     6061 ctggggggcg tattggcagg caccctctgc tgcggctctg ccggcgcatc gatcaggcgc
     6121 caccaccggc cttttgcccg gacgtttttg acccttaccg accccggtaa ggacgccgcc
     6181 cttgtcgtcg acggagaaac cggccgccca ctctacgcac gcaacgccga cgccctgcgc
     6241 catcccgcgt cgctcacgaa gatgatgacg ctttatcttt tgttcgagca gttgaagagc
     6301 gggcaaatga cgctcgccac ccccctttcc gtctcggaac atgccgcggt gcaggcgcgc
     6361 accaaactaa atctccgccc cggcaccagc ctgcccgtgg acacggcgat caaagccatt
     6421 gtggtgcttt cggccaatga tgtcgcggtc accatcgcgg aagcgatcgg cggcactgag
     6481 ggtcatttcg cggaattgat gacagcgaag gcgcgcgcgc tgggcatgac gcatacctat
     6541 tacgataacg catccggact gccggatccc ttgcagatca cttcggcgag cgatctggtc
     6601 atcttggcgc ggcatctcgc ttacgatttc ccccaatatt tccattactt ctcgacatat
     6661 tcgttcggct atcgcggggc gacctacttt acgcatgaca atctgatcgg aagatacgaa
     6721 ggcgccgatg gaataaagac cggctatacc ggggcttcgg gcttcaatct cgtttcgtcc
     6781 gtggtgcgca acggcgttca cattatcggt gtggtcatgg ggggcgtcac ggcgcgccgg
     6841 cgcgacagcg agatggtccg gctgctcgac gacacgtttg cgtccattgc gaagaacccg
     6901 cggctcgcgg cgcgcgagac cattccgtgg acggcaatgg ccagcaatac cgagaccagg
     6961 ccggtaattg ccggattcac gctcggaaac gcgggcaata cgctgccgcc ggcgctcccc
     7021 gcgctcccgg cgaaccggtc acttcttccc ggcccgtcgg ctgtatccgc cgatgacgaa
     7081 gacgccgccg aatcgcggcc cgatgcagca tatgagtcgc taaaaaaccc cccggaggcg
     7141 gtcgttacgt cagcccccgt atccaatcac ccggcggtag ccaatcatcc agggccggcc
     7201 gcgagcccgg tcggcagcgc tccggccgcg atttctctgc cagttgcgca agctccgatg
     7261 tcgctcgctg tgacgcctcc gcattcggag gcgaaagcgg cgaccgccac cgaattgcgg
     7321 gccaacacgc ctcggccgca gcctaaaccg agcatggagc tcgccgatta tcagccaccg
     7381 gcgctcgagc ctctcgttct ccatccgcgc gaccctgccc gacgcgacag cgcgccgccg
     7441 gaaattcccg tttcccgccc tctctcggcg gcactcggga aggctgactg gacgatccag
     7501 atcggagcct tcgacgacgc ggccgatgcg aaagccgaac tcgccgccta tgccggacgc
     7561 tcgacggatg tgctgggtca ggcggctcgc atcgtggcgc cgatacaatc ggccgagggc
     7621 catgtcctct accgggcgcg gttcggtccg ttcatcgagc accaggcgcg cgaggtgtgc
     7681 caactcctga ccgtccgcgg cgaaacatgt ttcgctgctc tttccgttcg ccaatagaac
     7741 tcaacgttcc cgagccgggc gcgcagcgtc gccttcgtca cgggccgcgc ccattcagca
     7801 atacctctct tgcttcgttg atccgcgccg ccagatagct ggtgccgccc ctgtccgggt
     7861 ggatttgaag gatcagccgt ctgtgtgcgg cgttgatttc gtcccgtgtt gctcccggtt
     7921 ccagtcccag cagtttcaag gcctcggcac gcggcatcgc ggaaccggct cgcgctgtcg
     7981 gctcctcgga agatgtcgag cggccgccgc tgaagggcca gccgtctggc caggcacggc
     8041 cgccggtaaa aaggccccag gcaaagcttc cggcaagcat ggcgagaccc acgcgatcga
     8101 tggcggcgag tccgaccgcc gcacacagaa gaaccgcggc gccggaatag cgcacgcccc
     8161 tcgtcaccag gcgtgtatcc gctccggcaa aggcgcgcag cagcatcagg atcgcgacaa
     8221 gcgcgacacc accggcaaga accgcaatca ttcaggcaat cacatgggtg cgtcgcgaga
     8281 cggcagtagc ggcatcgccc tgcgtcgccg ctcggcgcga atgatcacca gggccgggat
     8341 gctgaccgcg atgacgccga gacacgccgc caattcgaca aattttcccc tctctgccgc
     8401 agccgctcca gctatggcgc cggcagggcc ggcggccacg atcgcaacaa gcgccaacgc
     8461 ctttagcgat ctcgacacct gggaatcctc ctcggcgcat cgccgtctcg ccagaatatg
     8521 tgaggacgac atcgatccgg aaacgtcgca ggcccatgcc actttccgaa gcttttggca
     8581 atcgacccga ttccaggcct gagccaggaa ctcggcactc cggtccggcg ccttcgcggc
     8641 ccaggaaccc gcgtttcgat ggttcgttag gttcgggtgc gggccgcggc gccaacgggc
     8701 gggcctcgcc cgaggggctt cgggaggaat tgtcatgaac accaccatca gcccggcgct
     8761 tgcgatcgtg ctggcggcca tcgtcatcat cgtgctgctc gccaccattg cgttcatctg
     8821 gtggcgggag cgcaggtcga agcagctgcg ctcgcgcttc ggccaggaat atctacgcac
     8881 gctcgaggag atcggcagcc gccacgaagc ggaggcccgt cttcacaggc tcgagaagcg
     8941 cgtcgagtcc ttttcgattc gtgcgctgcc gccggaagag aaggcccgct acgcagcctc
     9001 ttggcgaaag gtccaggccg atttcgtcga cagtcccggc gaagcgctca accgtgccga
     9061 cgagctgctc ggctcggtga tgaaggcgcg cggctacccg gtcagcgatt tcgagcagcg
     9121 ctccgccgat ctttcggtcg atcacgcggc ggtggtgcag aactaccgcg cggcgcacga
     9181 cattgcgctg cgccacaagc gcggcgaagc cgacaccgag gcgctccgcc aggcgatgat
     9241 ccactaccgc gcgctgttcg ccgaactggt ggacgagacg gatgaggcgc gggcgaaagc
     9301 ctcgtagatg aaatcccctc accccgagct tggtccaccg gtttaacggc gggaagggtg
     9361 agccgacgaa cgcaacttca ggaattcaga tgatgaacga agcactgcag gtggacaagg
     9421 aatacgggga ggaacgccac aaggaactgg cgaccgcgaa cctcgccgga acgaagccgc
     9481 tcgatgtggc ctggcgggaa tcccgcgagc gggagaatag cggcgccgcg aactggtccg
     9541 gcgacgacga gaaagcggcg ccgctgcttt tggccgagga cgccgatcgc atgcgcgcag
     9601 aatgggattc catccaggcg ggcttcgtcg acgagccgcg ggccgcggtg gagaaggccg
     9661 acaatctggt cgccgccacc atgaagcgcc tcaccgaaaa tttcgccgag gaacgctcga
     9721 agctcgaaag ccagtgggat cgcggcgacg acgtctcgac cgaagatctc cgcatcgcgt
     9781 tgcggcgcta tcggtcgttc tttcagaggc tgctgtcggt ctgacaaacc ggcctctcag
     9841 ctt
//
LOCUS       DASZMC010000122         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2810864,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000122 DASZMC010000000
VERSION     DASZMC010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2810864"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VG274_02425"
     CDS             <1..455
                     /locus_tag="VG274_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWE05533.1"
                     /translation="TVTGWRVGWALASPDLTDSIRKIHDFLTVAAAAPLQAAGVTALS
                     MSRDFYAGIAREYQKKRDLMLASLTDAGFRCSRPEGAYYIMTDISRFGFPDDISFMAH
                     MAEHAGVVGVPGSCFFKDPASGAQNIRFCFCKKYETLELASSRLKRLA"
     gene            523..897
                     /locus_tag="VG274_02430"
     CDS             523..897
                     /locus_tag="VG274_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05534.1"
                     /translation="MPAFLLPEKTASGDGWGAHVILDQPHTRQLQLTLGITRIRERES
                     LQVQVCGSADGEKWRPLAAFPRKSFCGTYQLNLDLSRHSDVKYLRTEWKMHRWTQDET
                     KPVFSFYVWAESLHPKALRAAS"
     gene            complement(1038..>1908)
                     /gene="larB"
                     /locus_tag="VG274_02435"
     CDS             complement(1038..>1908)
                     /gene="larB"
                     /locus_tag="VG274_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWE05535.1"
                     /translation="GSQERVRRKEEEEEEEKNRAFEDARGPKPGATMSFRYSGKYMDE
                     KQLRFLLDEVRSGATGIDAAMERMRHMPFEDLGFAKVDHHRAIRHGMPEVIFGKGKTP
                     DQISSIAAALLAQAQNVLITRAEPGVAQRLLQEHNGGEYFPASGALRFWGDREMHGKG
                     KIAVVCAGTSDLPVAEEAQVTAEVMGNEVVTLHDVGVAGIHRLMSSRDRLAEARVVIV
                     CAGMEGALPSVVGGLVNVPVIAVPTSVGYGASFQGLAALLGMLNSCASNVTVVNIDNG
                     FGAGYAASLMNRL"
BASE COUNT          355 a          636 c          518 g          399 t
ORIGIN      
        1 atacggtcac cggctggcgc gtcggttggg cgcttgcgag cccggacctc accgattcca
       61 tccgcaagat ccacgatttt ctgacggtcg ccgccgccgc gcccctgcaa gccgccggtg
      121 taaccgcgct ctccatgtcg cgagacttct acgcaggcat cgcgcgcgaa tatcagaaaa
      181 agcgcgattt aatgctcgcc tcgctcaccg atgcgggctt ccgctgctct cgacccgagg
      241 gcgcgtatta catcatgacg gacatttccc gcttcggctt cccggacgac atcagcttca
      301 tggcccacat ggcggaacac gcaggcgtcg tcggcgtgcc cggctcgtgt ttttttaaag
      361 atccggcctc cggcgcgcaa aatatccgct tctgcttctg taaaaaatat gagacgctgg
      421 aattggcgag cagtcgcctg aagcgcctcg cctgaaactg actgggtgaa ccccaaaagc
      481 ttcggtgacg gaggcttcgc ccggcgaaag gaggcttgta tgatgcctgc ttttctactc
      541 ccggagaaga cggcctctgg ggacggttgg ggcgcgcatg tcattctcga ccagccccac
      601 acacgccaac tacaactcac gctgggaatt acccgcatcc gtgagcgcga aagcctgcaa
      661 gtgcaagtgt gcggatcagc cgacggtgag aagtggaggc cgctggccgc cttccctcgc
      721 aagtcgttct gcggtacgta tcagttgaat ctcgacctgt cccggcatag cgatgtaaag
      781 tatcttcgga cagagtggaa gatgcatcgc tggacccagg acgagaccaa gcccgtgttc
      841 agcttctacg tctgggccga gagcctgcat ccgaaggcgc tgcgcgctgc gtcgtagccg
      901 aattcatttt ccccttatcg gccatttccg gtcgacgtga aatcgatcga tattttgtga
      961 ccctgttcct gcgcgaggga cggcacgagc agagcaacgc aaccgatgag gagtgcgcag
     1021 atctgctaac acagtctcta caatcgattc atcaggctcg ccgcataacc cgctccgaaa
     1081 ccgttgtcga tgttcaccac cgtcacgttg ctcgcgcagc tgtttagcat ccccagcagc
     1141 gcggccaggc cctgaaaact cgcgccataa ccgacgctgg tcggcacggc gatcaccggt
     1201 acgttgacca atccgcccac cacgctgggc agcgcgcctt ccataccggc acacacaatt
     1261 accacgcgcg cctcggccag ccggtcgcgg ctgctcatca gccgatgaat gcccgcgacg
     1321 cccacgtcgt gcagcgttac cacttcgttg cccatcacct cggccgtcac ctgcgcttct
     1381 tcggccaccg gcaggtcgct ggtgcccgcg cacaccacgg cgatcttgcc tttgccgtgc
     1441 atctcgcggt cgccccagaa tcgtaaagcg cctgacgcgg gaaagtattc gccgccgtta
     1501 tgctcctgca acaatctttg cgccacgccc ggctcggccc gggtgatcaa tacgttttgc
     1561 gcctgcgcca agagcgccgc cgcgatggag gaaatctgat cgggcgtttt gcccttgccg
     1621 aagatcactt ccggcatgcc gtggcggatc gcccggtgat gatccacctt ggcgaatccc
     1681 aaatcctcaa acggcatgtg ccgcatgcgc tccatcgccg cgtcaatgcc ggtggcgccg
     1741 ctgcggacct cgtcaagcag gaatcgcagc tgtttttcgt ccatatattt tccagaatag
     1801 cgaaaactca tggtggctcc cggttttggt ccgcgagcgt cctcaaaagc cctgtttttc
     1861 tcctcttctt cctcctcttc ttttctcctg actctctcct gactcccg
//
LOCUS       DASZMC010000123         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4192659,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000123 DASZMC010000000
VERSION     DASZMC010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4192659"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..413)
                     /locus_tag="VG274_02440"
     CDS             complement(153..413)
                     /locus_tag="VG274_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HWE05536.1"
                     /translation="MDLTLRIPDDLAERLGAAGELERRALEALALEEFRLGHLSRLEI
                     RELLGFATRGELDAFLTAHGTFGTYTREDLERDRQDLQCLGY"
     gene            complement(687..935)
                     /locus_tag="VG274_02445"
     CDS             complement(687..935)
                     /locus_tag="VG274_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWE05537.1"
                     /translation="MREIPLSEIKDDLSHFLREAEKQEIVITRHGKPAGVLIGFESED
                     DWFEYRLENDPRFLRRIEKARAGIRAGRGVRLEETDSG"
     gene            complement(932..1225)
                     /locus_tag="VG274_02450"
     CDS             complement(932..1225)
                     /locus_tag="VG274_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWE05538.1"
                     /translation="MPLDIILAPEAVEDLKALKANVRTAVRTAIEDHLRHEPRKARRS
                     RIKRLRGLEKPQFRLRAGEVRVFYDVSARTVEVLAIVAKSEAKTWLAQSGRPE"
     gene            1337..1594
                     /locus_tag="VG274_02455"
     CDS             1337..1594
                     /locus_tag="VG274_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWE05539.1"
                     /translation="MQVLIAKSAVKALRKMQPEKAKDIREAISKLARDPAAPNNNVKA
                     LKGIKDGYRIRVGDRRVSYLSDRASQTLKVFEIAPRGGAYR"
     gene            1591..2064
                     /locus_tag="VG274_02460"
     CDS             1591..2064
                     /locus_tag="VG274_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWE05540.1"
                     /translation="MTSRNSISRELRVRLAGARKKTIARHLRAVAPRLQLAESGDAAD
                     FVMLPSEDYERLLDEADERSAKMAFERTRDEEFVPLEIADRLLMRENPVHVWRAHRGL
                     TLAQLGERAGLSRGYLSDIENGKRSGTVGTLRSIARALGVDVDDLVAPVQQRRTG"
     gene            complement(2090..2344)
                     /locus_tag="VG274_02465"
     CDS             complement(2090..2344)
                     /locus_tag="VG274_02465"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HWE05541.1"
                     /translation="MARNAPHPSQSKPRHGRDNSPANSEFDLSGMRFVRFELKPKDKS
                     INLRLPESLLNAVRARAKRARMPYQRFIRMALERAVAAKR"
     gene            complement(2319..2708)
                     /locus_tag="VG274_02470"
     CDS             complement(2319..2708)
                     /locus_tag="VG274_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05542.1"
                     /translation="MWRKIGSVVAGLVAWAVIVSVIDRLMRLAWPEYAAALPTLHFTL
                     PMMIARLSEAAVATVVAGWFARWIARTPLWLAWVQGALILLPFLPVHYRLWHSFPVWY
                     HLTFLVPLIPLTLLGASLANRAERTSP"
BASE COUNT          594 a          975 c          995 g          561 t
ORIGIN      
        1 tgtagggacg cttgcgaaga acctgctctt cgaaatatcg cgtcgtcttc atcggctgct
       61 aaactcgatc gacctcggca acaacgccac atcgccggtg agcacgaagt aatgaatcgg
      121 tccggtatcg gcgacgacga gctgcatgcg cctcaataac ccaggcattg caggtcttgc
      181 cggtcacgct ccaggtcctc tcgcgtgtaa gtgccgaagg tcccatgggc ggtgagaaat
      241 gcatccagct ctccgcgggt ggcgaagccg agcagctccc ggatttccaa tctgctcaga
      301 tgcccaagcc gaaactcttc aagcgccagg gcttcgagcg cgcggcgctc aagctcgccc
      361 gcggcgccca ggcgttccgc caaatcgtca gggatgcgaa gcgtcaggtc catgacgtta
      421 cactatatgc agatgccgca tggggtcaaa gtatcgacat cggtcgggct cacgttagct
      481 cgtgcggggg ggccgataaa acgtcccgcc caattcctgg acgcacgtgt ttcctgggtc
      541 ccggctctcc gctgccgcgc ctgcctgcgc gaagccgaag cttcgcttcg gcgaaggcag
      601 atcggtcggg atgacatggt gtttttgcgt gcgcaaggat cgcgcgccag gcaatgtgca
      661 ccctcggact tttccgtgcc gccggtctat ccggaatctg tttcttccag acgaacgccg
      721 cggccggcac ggatgccggc gcgggctttt tcaattcgcc gcaggaaacg cggatcattt
      781 tcaagccggt attcgaacca atcatcttcc gattcgaatc cgatcagcac gccggccggc
      841 ttgccgtggc gcgtgatgac gatctcctgc ttctcggcct cgcgcagaaa atgcgagaga
      901 tcgtccttga tctcggagag tggaatctcc ctcattccgg ccttccggac tgcgcgagcc
      961 atgtcttcgc ctccgatttc gcgacgatcg ccaggacttc aactgtccgg gcagaaacgt
     1021 cgtagaacac cctgacttcg cccgcgcgca acctgaattg cggcttttcg agcccgcgca
     1081 agcgcttgat gcggcttcgc ctcgccttcc gcggctcatg ccgcaggtga tcctcgattg
     1141 ccgtccgcac ggccgtccgg acattggcct tcagggcctt caaatcctcc acggcttccg
     1201 gagccaggat tatgtcgagc ggcattctgg ccagataata gccagaattt tcctgccgtc
     1261 aaaccaggca agcccgcccc ataccggtcg aaaataatcg ccttgcgaac gagttcgctt
     1321 tctgcgaaca tgcacgatgc aggttttgat cgccaaatcg gccgtgaaag ctctgcggaa
     1381 gatgcagccg gagaaggcga aggacattcg ggaggcgatc agcaaactgg cccgcgaccc
     1441 tgccgcgccc aacaacaacg tcaaggcgct gaaggggatc aaagacggct accgcatcag
     1501 ggtcggcgac cggcgggtgt cctatctcag cgatcgcgca tcgcagacgc tgaaagtgtt
     1561 cgagattgcg ccgcgaggag gcgcctacag atgacaagca ggaattccat cagccgggag
     1621 ctgcgcgtgc ggctcgcggg cgcccgcaag aaaacgattg cccggcatct gcgcgccgtc
     1681 gcgcccaggc ttcagcttgc ggaaagcggc gatgccgcgg atttcgtgat gctcccttcg
     1741 gaagactacg agcgcctgct cgatgaagcg gacgagcgga gcgcaaagat ggccttcgaa
     1801 cggacgcggg atgaggaatt cgttccgctg gagatcgcgg atcgtctcct gatgcgagaa
     1861 aatcccgttc acgtgtggcg cgcgcatcgc gggttgactt tggcccaact gggcgagcgc
     1921 gccggattga gcaggggtta cctgtccgat atcgaaaacg gcaagcgaag cggcactgtc
     1981 ggcacgctgc gctccatcgc cagggcactt ggcgtcgacg tcgacgatct cgttgcgcct
     2041 gttcaacaac ggcggacagg gtgagccgct ggaacgggga agccgcctcc taccgcttcg
     2101 ccgccaccgc ccgttcgagc gccatgcgga tgaagcgctg gtagggcatc cgggcgcgct
     2161 tcgcacgagc acgcacggcg ttcagcagcg attccggaag ccgcaggttg atcgacttgt
     2221 ccttcggctt cagctcgaag cggacaaaac gcatccccga cagatcgaat tccgaattgg
     2281 ccgggctgtt gtcccggcca tgacgaggtt tggattggct agggtgaggt gcgttccgcg
     2341 cgattggcga ggctggcgcc caacagggtc agcgggatga gggggacgag gaaggtgagg
     2401 tgataccaga cgggaaaact gtgccagagc ctgtaatgca cgggcaggaa ggggagcagg
     2461 atcagcgcgc cctggaccca ggcgagccac agcggcgtgc gggcgatcca gcgtgcgaac
     2521 catccggcca cgaccgtggc gaccgcggct tcgctcagcc gcgcgatcat catcggcagc
     2581 gtgaaatgga gcgtgggaag cgccgcggcg tattcgggcc acgcaaggcg catcagacgg
     2641 tcgatgacgc tgacgatcac cgcccacgcc acgagccccg ccaccaccga accgatcttc
     2701 cgccacatcg cccccgcctc ttctgtccgg attcaaaaca tcattttatc ctgtcatggc
     2761 ccgcgcaata aagcttgtcc tccggccgcg cttcgcgcgg accggggggc gggccacccc
     2821 gttggaagct acggggagag aagtcatgag caagaaccgg cgttcgctcg cagcccctat
     2881 cgggtttcgt ctgggtggcc cgctctccgg cgggcgatga cagtgttttt tggtcccccg
     2941 cgcggaggat ggatttgcgc aagcgtgaaa tgaaacgggc gaagcggtcg gcgacgcagg
     3001 cggtggggcg atgcgtgtgc ggcgtggtgc gattcgagat cgacgtgccc gccgtctggg
     3061 cgtggcacga tcactcgcag gccagccggc acgcgcaggg ctgcgcctat gccacctatg
     3121 tcggc
//
LOCUS       DASZMC010000124         5264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3078476,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000124 DASZMC010000000
VERSION     DASZMC010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078476"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VG274_02475"
     CDS             <1..300
                     /locus_tag="VG274_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05543.1"
                     /translation="HCLAEFYSVVTRMPGKHRASPDEALLFLHDALERLSIIALNGDE
                     YLQVLEDAAAAAISGGTIYDAIIARCALKARAQMIYTWNIRHFSLFGSQIASREP"
     gene            complement(548..964)
                     /locus_tag="VG274_02480"
     CDS             complement(548..964)
                     /locus_tag="VG274_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWE05544.1"
                     /translation="MRQSEGVFVDSGAWIALALVKDPLHGQAREQWTLLRETGAKLHT
                     SVPVIVETFTFLDRNTNRDVALVWKESIYKPGAVKIVPCELRDLESAWEYFRRADLHK
                     LSVVDATSFVVMKRLRIRLAYTFDHHFAVAGFRLVG"
     gene            complement(964..1173)
                     /locus_tag="VG274_02485"
     CDS             complement(964..1173)
                     /locus_tag="VG274_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWE05545.1"
                     /translation="MEKTQVYLRRDELDALRAAAARSGRSVAEVIREAIRKVVLKPGT
                     AGPVAIWDGAPRRTSLEHDSIYEEP"
     gene            1203..2600
                     /locus_tag="VG274_02490"
     CDS             1203..2600
                     /locus_tag="VG274_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE05546.1"
                     /translation="MSILLVSWPVRRGERAEPIRTARLCLAAFVAFLVCATYAAAQSP
                     KLVVYRGAALIDGTGAALRRDVAIVVDGERIRAVVPDAQAAAYARGATVIDVNGFYAL
                     PGLVDSHVHLATAPNRRYAEALLRRDLYSGVTAVRDMAGDARLLADLSRAALLNEIPS
                     PDISYAALMAGPEFFRDPRTAEAARGAVPGEVPWMRAITTSTDLRLAVAEAHGTGATA
                     IKIYADLPGTLVRNIVDEAHRQTLLVWAHAAVFPASPREVIDSGVDVVSHVCMLAYQA
                     SEQMPRAYHNRAPVEEAKFTGDNPQMDAIFADMKRRGTVLDATLYVYDSMWRVPNAQP
                     PPYCTLNLAEKLAAEAHRAGVAISAGTDAPGDWNDPYPSLDGELSLLAHGAGFSPMDA
                     IVAATRIGAMTIGKSRETGTVEPGKLANFVFVSKNPLDDIANLKRVTMTVKRGRLYRR
                     SAYRPISKDEAQGEF"
     gene            2646..3326
                     /locus_tag="VG274_02495"
     CDS             2646..3326
                     /locus_tag="VG274_02495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWE05547.1"
                     /translation="MTLRGTMISMPMSDGAGVGVYRVEPVGPRRGGLVLIQEIFGVTD
                     HIKEQCDLYAAQGYEVLAPSLYDREEPGFNVGYGAEDMQKAMRVARGEHPFDLSVKDA
                     QTCIDALKAKGPVVITGYCYGGSVTWAAACRCPGLAAASGYYGGNIGQMAEQNPKCPT
                     ILHFGRHDHGIPMEVIEKIKRLHPDVMVYLYEAGHGFNSDRRADYSEVAAKLARERTL
                     ELFRTHGG"
     gene            3364..3660
                     /locus_tag="VG274_02500"
     CDS             3364..3660
                     /locus_tag="VG274_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWE05548.1"
                     /translation="MNVTGENDELTKRIIGFAIEVHRSLGPGLLESAYEECLCLELKR
                     GDVTFARQVALPVTYKGVKLDCGYRLDVVVESRLIVEIKAVERLMPIHEAQLLT"
     gene            complement(3845..>5264)
                     /locus_tag="VG274_02505"
     CDS             complement(3845..>5264)
                     /locus_tag="VG274_02505"
                     /inference="COORDINATES: protein motif:HMM:NF006192.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HWE05549.1"
                     /translation="EERLKQGRKRLAPASMPQRLARTADIAPILRGACAITRDPTSLK
                     LRRTSEPAGTIKRQILCFRKSPSILDYVNGAELARYSQQGVVTPDHTIRTKNWPLLAP
                     APEEGKLEAWKTSVEEAVAAFVRHYHDYFARNNARAEPKKAELDPLPRVVLVPGKGLF
                     GLGASAKDAAIAADIAENTIEVIADAEATGAYRPISEADMFDVEYWSLEQAKLGKAAE
                     KPLARQVAVVTGGGSGIGAATASALARAGAEVAVLDRDRASASFVARDCGKTAIAVVC
                     DVTSPESVKSAFDEVAKTFGGIDIAVSNAGAAWQGTIGTVDDAALRESFELNFFAHQT
                     VAQNAVRIMKAQGTGGCLLFNVSKQAVNPGRDFGPYGLPKASTLFLMKQYALDHGRDG
                     IRSNAVNADRIRTGLLTDDMIASRSQARGLPESGYMSANLLGREVTAEDVADAFVYLA
                     TATKTTAATLTVDGGNIEASLR"
BASE COUNT          983 a         1655 c         1606 g         1020 t
ORIGIN      
        1 cactgcctgg ccgaattcta ttcggtcgta acgcgaatgc ctgggaagca tcgtgcaagt
       61 cccgacgagg ccctgctctt tcttcacgat gccctggagc gcctgtccat catagcactg
      121 aacggggatg aataccttca ggtgctggag gatgccgccg cagctgcaat ttccggcggc
      181 acgatctatg acgcaataat tgcgcggtgc gccctcaagg ccagggcgca gatgatatac
      241 acatggaaca tccggcactt ctcgcttttc ggatcgcaga ttgcttcgcg cgaaccctga
      301 caagcggcgc ctgctgcagc agatggatca gctcgcgccg aactcgccaa gaaaaagccc
      361 cgccggatca tatgtcttcc gcaccgtgag ccggcgaacc gtggaaaaat acctcgagcg
      421 cattttcccc aacttggagt cgagacacgc tccgccgcaa cgagctttgt tcagcagttc
      481 ccgaccgacc gtccttggta ctccgatgag gagagctgct cacctcatct cgtgcaggag
      541 acggatccta acccacaagc ctgaagccgg ctacggcaaa gtgatgatcg aacgtatagg
      601 cgagccgaat ccgcaagcgc ttcattacga caaagctcgt cgcatcgacc accgatagct
      661 tgtgcaagtc cgcccgtcga aaatattccc acgcagattc caggtcgcgc agttcgcagg
      721 gaacgatttt gaccgcgccg ggcttgtaga tggattcctt ccataccaaa gccacgtccc
      781 ggttggtgtt gcggtcgagg aatgtgaagg tctcgacgat aacaggtacg gaagtgtgga
      841 gctttgcgcc ggtctcccgc aagagagtcc attgctcgcg cgcctgcccg tgcagcggat
      901 cttttactaa cgcgagcgcg atccaggcgc cgctatcgac gaacacgcct tccgactgcc
      961 gcatcagggc tcttcgtaaa tgctgtcgtg ctcaagtgag gttcgcctcg gcgcgccatc
     1021 ccagatcgca accgggccgg cagttccggg tttgagcacg actttccgga tcgcctcgcg
     1081 gatcacttcg gctacactgc gccctgatcg ggcagcggcg gcgcgcaacg cgtcgagctc
     1141 atcccggcgt agatatacct gggtcttttc catgtgtgtc actttgacct tggagttgac
     1201 atatgtcaat attattggtt tcctggcctg tgcggcgggg tgaaagggcg gaaccgattc
     1261 gcaccgcaag gctttgtctc gcggcatttg tggcattcct cgtttgcgcg acatacgcgg
     1321 cggcgcaatc gccgaagctt gtcgtctacc gtggcgctgc actgattgac ggaacgggag
     1381 ccgcgctcag gcgcgatgtc gcgatcgtgg tggacggcga acgcatccgt gcggtcgtgc
     1441 ccgacgccca ggccgcggcc tatgcgcgcg gcgcgaccgt aatagacgtg aacggcttct
     1501 acgcgttgcc cggtctggtc gacagtcatg tgcatctggc gactgctccg aaccgccgct
     1561 acgcggaagc gctgctccgg cgtgacctgt acagcggcgt caccgccgtc cgcgacatgg
     1621 cgggcgatgc gcgcctgctg gccgatctgt cgcgcgcagc gctgttgaac gagattccgt
     1681 cgccggatat ctcctatgcg gcactgatgg cgggtccgga attcttcagg gatccgcgca
     1741 cagccgaggc ggcgcgcggc gcagtgccgg gtgaagtgcc ctggatgcgc gccattacga
     1801 catcgaccga tctcagactc gctgttgccg aggcgcatgg caccggcgcg accgcgatca
     1861 agatctacgc ggatctgccc ggcacgctcg tcaggaatat cgtggacgaa gcgcaccggc
     1921 agacccttct cgtgtgggcg catgcggcgg tctttccggc gagcccccgc gaagtgatcg
     1981 actccggcgt cgatgtcgtc tcgcatgtct gcatgctcgc ctatcaggcg tcggaacaga
     2041 tgccgcgcgc ctatcacaac cgcgcgccgg tcgaggaggc gaaattcacc ggcgacaatc
     2101 cgcagatgga tgccatcttc gccgacatga agcgccgcgg cacggttctc gatgcgactc
     2161 tctatgtgta tgactcgatg tggcgcgttc cgaatgcgca gcctccgcct tattgcacgc
     2221 tgaacctcgc ggaaaagctg gccgcggaag cccaccgcgc cggggtcgcg atctcggcgg
     2281 gcaccgacgc tcccggcgac tggaacgatc cgtatccgtc gctcgatggg gaactttcgc
     2341 ttctcgcgca cggagcggga ttttcgccga tggatgcgat tgtcgcggca acgcggattg
     2401 gcgcgatgac gatcggcaag tcccgggaaa cgggaacggt cgagccgggc aagctggcga
     2461 atttcgtttt cgtttcgaaa aatccgctcg acgatatcgc caatctcaaa cgcgtgacga
     2521 tgacggtcaa gcgcggacgg ctctaccgcc gctccgccta ccggccgatc tccaaggacg
     2581 aagcgcaggg tgaattctga gcgcgcctgc gtcatgatgc gccaaatccc attgcggaga
     2641 acgacatgac cttgcgcgga acaatgattt caatgccgat gagcgacggc gccggggtcg
     2701 gcgtctaccg tgtcgagccg gtggggccgc gccgcggcgg gctggtgctg atccaggaga
     2761 tcttcggtgt caccgatcac atcaaggagc aatgcgacct ctacgcggcc cagggctacg
     2821 aggtgctcgc tccgtctctc tacgaccgcg aagaacccgg attcaacgtg ggctacggcg
     2881 cggaagatat gcagaaagcg atgcgcgtcg cgcgcggcga gcatcccttc gatctttcgg
     2941 tgaaggacgc acagacctgc atcgatgcgc tcaaggcgaa aggacccgtc gtcatcaccg
     3001 gctattgcta tggcggttcg gtgacctggg ccgccgcctg ccgctgcccc gggctcgccg
     3061 cggcgtcagg ctattacggc ggcaatatcg ggcagatggc cgagcagaat ccgaaatgcc
     3121 cgaccatcct gcatttcgga cgccacgatc acggcattcc gatggaggtg attgaaaaga
     3181 taaagcgcct gcatcccgac gtgatggtct atctctacga ggccgggcac ggcttcaatt
     3241 ccgaccgccg cgccgattac agcgaggtcg ccgccaaact cgcccgcgaa cgcaccctcg
     3301 aactcttccg cacccatggc ggataagaga ttcacaggga ggcagtgaga cggagagatc
     3361 gggatgaatg taacgggcga aaacgatgaa ctgaccaaac gaatcattgg tttcgcaatc
     3421 gaggttcatc gttcacttgg tccgggatta ctggaatctg cgtacgaaga atgcctttgc
     3481 ctcgaactga agcgcgggga tgtgacgttc gcgcgccaag tggcgctacc tgtcacatac
     3541 aagggtgtta aactggattg cggctatcgc ctcgacgtcg ttgtcgagag tcgcctaata
     3601 gtcgagatca aagcagtcga acgattgatg ccgatccacg aagcgcagct tctcacgtaa
     3661 ttgaagctca gcgaaatcgc cacaggcctg ctgctgaact tcaattctgt tgtgttgaag
     3721 gatggcatcc ggcgtatcgt gctgtgcgaa atcgatttga ggtgagacgg cgagaatagt
     3781 gttgcgcgcc aacggcgcgc caaaaacctc actgcctcac tgcctccctg tgaacctttc
     3841 ttcgtcaccg caggctcgct tcgatattgc cgccgtcgac ggtgagggtg gcggcggtgg
     3901 ttttggttgc ggtcgccaga tagacgaagg cgtcggccac gtcttcggcg gtcacttcgc
     3961 ggccaagcag attggcgctc atgtagccgc tttccgggag gccccgcgcc tggctgcgcg
     4021 aggcgatcat gtcgtcggtc agcagcccgg tacggatccg gtcggcgttg acggcattgg
     4081 aacggatgcc gtcgcggcca tgatcaagcg catactgctt catcagaaac aacgtcgatg
     4141 ccttcggcag tccataagga ccgaaatcgc ggcccggatt caccgcctgc ttcgacacgt
     4201 tgaacagcag gcagccgcct gtcccttgcg ccttcatgat ccggacggcg ttctgcgcga
     4261 ccgtttgatg cgcaaagaaa ttgagctcga acgactcgcg cagcgctgca tcgtccaccg
     4321 ttccgatcgt gccttgccac gcggcgcccg cgttggagac cgcgatatcg attccgccaa
     4381 atgtcttcgc cacctcgtcg aaggcggatt tgacgctttc gggactcgtt acgtcgcaca
     4441 caacggcaat cgctgtcttc ccgcaatcgc gcgcgacgaa ggaagccgat gcgcggtcgc
     4501 gatcgagcac cgcgacctcc gcgcccgccc gcgccagcgc acttgcggtg gcggcgccga
     4561 tgccggagcc gccgcccgtc acgaccgcca cctgccgcgc cagcggcttc tccgccgcct
     4621 tgccgagctt cgcctgttcg agcgaccaat attccacgtc gaacatgtcc gcttcggaaa
     4681 tcggccgata ggctccggtc gcctcggcgt ccgcaatcac ttcgatggtg ttttccgcaa
     4741 tatccgccgc gatcgccgcg tctttcgcgc tggcgccaag cccaaacaga cctttgcccg
     4801 gcaccagcac aacacgcggc agcggatcga gctccgcctt cttcggttcc gcacgcgcgt
     4861 tattgcgcgc gaaatagtcg tggtagtgcc tgacgaatgc agcgaccgcc tcctcgacag
     4921 aggtcttcca ggcctcgagc tttccctctt ccggcgccgg cgccagcagc ggccagttct
     4981 tggtgcggat cgtatgatcg ggggttacga cgccctgttg cgaatagcgc gccagttccg
     5041 cgccgttcac ataatcgaga atgcttggcg acttccggaa acacagaatc tggcgcttga
     5101 tggttccggc gggctcgctc gtccgccgaa gctttagcga agtcgggtcg cgtgtaatgg
     5161 cgcaggcccc gcgcaggatc ggcgcaatgt ccgctgtgcg cgcgagccgc tgcggcatgg
     5221 atgctggtgc gagacgcttc cggccctgct tgagccgctc ttcc
//
LOCUS       DASZMC010000125         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3078790,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000125 DASZMC010000000
VERSION     DASZMC010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078790"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..>1585
                     /gene="bshC"
                     /locus_tag="VG274_02510"
     CDS             113..>1585
                     /gene="bshC"
                     /locus_tag="VG274_02510"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HWE05550.1"
                     /translation="MQPACIRHTDLPGTSRLFADFSYHFDKVAGFYRHNPQDPDALKN
                     AAAAIDYPADRRSALVKALAAQNAGGGDRSSESLRKLARPGTLAVVTGQQVGLFGGPA
                     YTIYKALTAVRLAKRLSDQGTPAVPIFWLATEDHDFAEVNHAWMFDSASQSQLLRAEA
                     NGHSARQRPVGWIAIDKPPVEELRGVLKGMPFADEVVAAVEQAYAPGASMGASFQALM
                     QSLTRNLGLIFLDPLDPAIRAIAAPLIAKAVRSAKEIKPRLIERDKQLEAAGYHAQVH
                     VDAQTSLFFLLEKGERVPVRRKDSDYSDLADRAAEVSPNALLRPVMQDYLLPTVAYIG
                     GPGELAYLAQSEVLYDALLGRMPVMMSRSAFTLLDARSVKLLERYKLTVEQVMVPEHE
                     LADRVARALVPQELQGSFQTAASNVTAEIDRLKAQFEGFDPTLAAALGTSRAKIAYQL
                     EKMRAKTEREALRRNRQATSDAHHLGAMLFPHRHLQERFYS"
BASE COUNT          297 a          468 c          523 g          297 t
ORIGIN      
        1 tcgcgatctt taacgcaccg gcaatctcat attctcgcgc aagccaccgc aaaatattcg
       61 gcaactatcg ccgaagctgc ttcatctgtt acgcttggac cgttcgcccc gtatgcaacc
      121 ggcttgtatc cgccacacag acctccccgg aacatcccgg ctgttcgccg atttttctta
      181 tcattttgat aaggttgcag gcttttaccg gcataatccc caggatccgg acgccctgaa
      241 gaatgccgcc gccgctatcg attatcctgc cgatcgcagg agtgcgctgg tgaaagcgct
      301 tgccgcgcag aacgccgggg gtggcgatcg cagctcggaa tcgctccgga agctcgctcg
      361 gccgggaacg ctggcagtgg tcacggggca gcaggtagga ttgttcggcg gacccgccta
      421 cacgatatat aaagcgctga ccgccgtgcg tctggccaag cgcctgagcg atcagggaac
      481 tcccgcggtg ccgatattct ggctggccac agaagatcac gatttcgccg aggtgaatca
      541 cgcgtggatg ttcgacagcg cgagccaatc gcaattgttg cgcgccgagg cgaacggcca
      601 ttcggcgcgg cagaggccgg tgggctggat cgcgatcgac aaacctcccg tcgaggagtt
      661 gcgaggtgtt ctgaagggga tgccgttcgc cgatgaggtt gtcgcggcgg tggagcaggc
      721 atacgcgcca ggcgcgagca tgggtgcgag ctttcaggcc ctcatgcaat cgttgacgcg
      781 gaacctgggg ctgatctttc ttgatccgct ggacccggcg atacgcgcga tcgccgcgcc
      841 tctgatcgcc aaggctgtga ggtcggccaa agaaatcaag ccgcggctga tcgaacgcga
      901 caaacaattg gaggccgcgg ggtaccacgc gcaggtgcac gtggacgcgc agacctcgct
      961 gttcttcctg ttggaaaagg gtgagcgggt tcccgtgcgg cgcaaggatt ccgactattc
     1021 ggacttggcc gatcgcgcgg cagaagtctc gccgaacgcg ctgctgcggc ccgtgatgca
     1081 ggattatctg ttgccgacgg tggcctacat cggcgggccg ggcgagctgg cgtatctggc
     1141 gcagtccgaa gtgctgtacg acgcgctgct ggggcgcatg cccgtgatga tgtcgcgctc
     1201 cgccttcacg ctgctggatg cgcgctcggt aaagttgctg gagcgatata aactgaccgt
     1261 agaacaggtg atggtgccgg agcatgaact ggcggatcgc gtggcgcggg cgttggtgcc
     1321 gcaggagctg caaggctcgt tccagacggc ggcgtcgaac gtcaccgcgg agatcgatcg
     1381 gctgaaggcg cagttcgaag gcttcgatcc cacgctcgcg gcggcgctcg ggaccagtcg
     1441 cgcgaagatc gcctaccagc tcgaaaaaat gcgcgcgaag accgagcggg aagctctgcg
     1501 ccggaatcgg caggcgacca gcgacgcgca tcatctggga gccatgctgt ttccgcaccg
     1561 gcatctgcag gaacgttttt attcg
//
LOCUS       DASZMC010000126         4951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_612851,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000126 DASZMC010000000
VERSION     DASZMC010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612851"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /gene="gspG"
                     /locus_tag="VG274_02515"
     CDS             <1..355
                     /gene="gspG"
                     /locus_tag="VG274_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HWE05551.1"
                     /translation="GLLAAIATPMVLHYLDSAKVSTAKTEVSNLAAGLDLYKYDVGRY
                     PTTQEGLEALVSAPQGVDNWNGPYVKKTTKLSDPWGHPYNYRFPGDHGEFDLYSYGAH
                     ADVAKNDEKPPIANW"
     gene            513..986
                     /locus_tag="VG274_02520"
     CDS             513..986
                     /locus_tag="VG274_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWE05552.1"
                     /translation="MRSLSTAQARVRGGPHGTAGEAGFTLIEVLVALTILSISLATLL
                     AVFTQGLDRARESRNEASARVLAQSLLAQAKTSTNLAAGDSGGRTNGFYWHLRVAPYG
                     SAADLAAWQATPTEISAKVMWLGSSGGRRSVTLSTLRYVPKPGTGSQADSDSDSE"
     gene            983..1720
                     /locus_tag="VG274_02525"
     CDS             983..1720
                     /locus_tag="VG274_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWE05553.1"
                     /translation="MREIRALTKSCEAEAGFTLLELLIATTVLGFLSLLLFGGLQFGT
                     RVWEKAQSSTTDTNRVRAAQLFLSDEISHIYPGFTGLSPADRHVEFEGQDKRMTFLAA
                     SKSVAGAMDWVTIQAQPGKGSVSVVVTSEGELSNGHDTALRHTLIRGLKWFQISYYGA
                     PSPAGASTTTRSIANAGLGGNAAALASAPPQWTSVWIGQRRMPSLIRIRAGFADKAEW
                     PDLVVSPRIDVDESCVLDQLSKYCQGR"
     gene            1720..2823
                     /locus_tag="VG274_02530"
     CDS             1720..2823
                     /locus_tag="VG274_02530"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HWE05554.1"
                     /translation="MATPTFSDSVGQFRGLAGELVSEWRRELARFVREMLASPLARGG
                     ELVLLDIEADQVVVKLASGEDVHEIGRAAGEGESRAREISSLLAASELVPRGTTDVAV
                     ELPDDDVLRRRFELPAAGRSTLGRAVPFELERLSPIEADRLYYDFNVLESLKSKKQVE
                     IELRIVKRSTVDSAIASAHAAGLRVGAIRFAGDPREADWRSFPLDRRAWLGLKWRQWN
                     VLALGAAAIVLGIAVVFAAYLRGVSAAGALADELEAAGSRAAVVHRLLHDIGDVRAQI
                     EFPLAQKRAPFLLAILSELTRALPDGTWLTEFQLDGSKVHIQGFSKSASDVVGVVDRS
                     PMFANAQFMAPLQSAPNGLERFDLSFDVKGAHR"
     gene            2823..3404
                     /gene="gspM"
                     /locus_tag="VG274_02535"
     CDS             2823..3404
                     /gene="gspM"
                     /locus_tag="VG274_02535"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HWE05555.1"
                     /translation="MTAPAFLRGRKGAFLAVGAIVLLFALVFALPVAALFRAQADDRA
                     EALAQLAAYRAQEAQRPALEARLAALQQGAKSVPGLLTASSAALAQAQLQSDMKELID
                     RSGGGLLSAQLLPPTRVKGFDSVAIEYDLTIPVSRFSSLVYAVETHTPYYFVDSADFV
                     MPPNWRPNPQGQAQDPSMEMRWTIHAFRWRGSR"
     gene            3401..4069
                     /locus_tag="VG274_02540"
     CDS             3401..4069
                     /locus_tag="VG274_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05556.1"
                     /translation="MSLRLNPAQIALAALCGMLSILLGYMLFAPLPDIPAPAIQTHAD
                     PVDDAPPARFDAPPKQAFAEVDDRSVFNPLRVRVSGPSAPGAANTNSLPADLSLVGVI
                     LDGDTRLALFKSAAAPLAVGVSVGGSIEGWQVTRVGPDEVGMRSAAGEQAIKLAAGKP
                     SGQPPGQPVGGPGNSPLNAAQANAQARFQANMAQRNNPANNNANNNANNANNNANNSD
                     DDDN"
     gene            4378..>4951
                     /locus_tag="VG274_02545"
     CDS             4378..>4951
                     /locus_tag="VG274_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE05557.1"
                     /translation="MRRMHRVAVLTLAACAVPSALFAMGGGGYGGGFSSMRMPTRPDD
                     YSAALKLIHQERYAEAMPCLDRAVAQHPHNADILNYLGYTHRMVGDYPGSLAWYRKAL
                     TEDPDHKGAHEYLGELYLNMRDVASARTQLAELVRLCPDTCDERDVLTKSIDTWVAAN
                     PPAAMPATAATAAAPASPATPSTSTPPTSPP"
BASE COUNT          834 a         1680 c         1556 g          881 t
ORIGIN      
        1 gggcctgctc gccgcaattg cgacgcccat ggtgctgcat tatctcgaca gcgccaaggt
       61 ttcgaccgcc aagacggaag tgtcgaatct tgccgccggg ctcgacctct acaaatacga
      121 cgtcgggcgc tatcccacga cccaggaggg tctggaagcg ctcgtttcgg cgccgcaggg
      181 cgtcgacaac tggaatgggc cctacgtcaa gaagacgacg aagctctccg atccctgggg
      241 ccatccctac aattaccgct tccccggcga tcatggggag ttcgacctct acagctatgg
      301 cgcccatgcc gacgtggcca aaaacgatga aaagccgccc atcgcgaact ggtgaaggcg
      361 aagtgcgtcc atccctcacc tgcgcttcgg actggaccaa ccgtttcctg ctgcagccct
      421 ctcccacaag gggagagggg gcgcttcctg ttgcatcagc gccggcaaat cagcgcgtga
      481 gattccctct ccccttgagg gagaggggca acatgcgaag tctctccacc gctcaggcgc
      541 gggtgagggg tgggccacac gggaccgctg gcgaggccgg cttcacgctc atcgaagttc
      601 tcgtcgcgct caccattctg agcatctcgc tcgccaccct gcttgcggtc tttacgcagg
      661 ggctcgacag ggcgcgggaa agccgcaacg aagcgtccgc gcgcgtgctc gcgcagtctc
      721 tgctcgcgca ggccaaaacg tccacgaacc ttgcagccgg cgacagcggc ggccggacga
      781 acggcttcta ttggcacctg cgggtcgcgc cttacggctc cgccgcggac cttgcggcat
      841 ggcaggcgac ccccaccgaa atctccgcca aagtgatgtg gcttggcagc agcggcggca
      901 ggcgctcggt caccttgtcg acattgcgct acgtgcccaa accgggcacc gggtcgcagg
      961 cggattcgga ttcggattcg gaatgaggga gattcgcgcc ctgacgaaat cttgcgaggc
     1021 ggaagcggga ttcaccctgc tcgagcttct catcgccacc acggtgctcg gcttcctctc
     1081 gcttcttctg ttcggcggcc tgcaattcgg aacgcgggtc tgggagaagg cccagtcgtc
     1141 gacgaccgac accaaccgtg tgcgcgccgc gcagctcttc ctgtccgacg agatttcgca
     1201 catctatcca ggcttcaccg ggctctcgcc ggccgaccgg cacgtcgagt tcgagggcca
     1261 ggacaagcgc atgacgtttc tcgcagcgtc gaagtcggtg gcgggcgcga tggattgggt
     1321 caccattcag gcgcagcccg gcaagggcag cgtctcggtc gtggtgacga gcgaaggcga
     1381 attgtcgaac gggcatgaca cggcgttgcg ccatacgctg atccgcgggc tcaaatggtt
     1441 ccagatctcg tattacgggg cgccatcgcc ggccggagcg tcgaccacga ctcgttcgat
     1501 cgccaatgcg ggtttgggcg gcaatgccgc tgccctggca agcgcgccgc cccaatggac
     1561 ttcggtatgg atcggccagc ggcgcatgcc ttcgctgatc cgcattcgcg ccgggttcgc
     1621 agacaaggcc gagtggccgg atctcgtggt atcgccgcgc atcgacgtag acgagtcctg
     1681 cgttctcgac cagctttcga aatattgtca gggccgctga tggcgacacc aaccttttcg
     1741 gattcggtcg gccagttccg cggccttgcc ggcgagcttg tctccgaatg gcgccgcgaa
     1801 cttgcccgct tcgtgcgcga aatgctcgcc agtccgctcg cgcgcggcgg agagctggtg
     1861 ctgctcgata tcgaagcaga ccaggtggtc gtcaaactcg cttccggcga agatgttcac
     1921 gaaatcggcc gcgctgcggg cgagggcgag agccgcgctc gggaaatctc gtcgctgctg
     1981 gcggcatccg agcttgttcc ccgcggaacc accgatgttg ccgtcgaatt gcccgatgac
     2041 gatgtcttgc ggcggcgctt cgagcttcct gccgccggcc ggtccacgct ggggcgggcc
     2101 gttcccttcg agctcgagcg gctgagcccc atcgaagccg atcgcctcta ctacgatttc
     2161 aacgtcctgg aatcgctgaa atcgaagaag caggtcgaga tcgaactgcg catcgtcaag
     2221 cgcagcacgg tcgattccgc catcgcctcg gcccatgccg ccggcctgag ggtcggcgcg
     2281 atccgcttcg ccggcgatcc gcgcgaggcg gattggcgat cctttccgct cgaccgccgc
     2341 gcatggctcg ggctcaaatg gcgccagtgg aacgtgctgg cgctcggcgc cgcggcaatc
     2401 gtgctcggca tcgccgtcgt gtttgcggcc tatcttcgcg gcgtttcggc cgcgggcgcg
     2461 ctggccgacg aactggaagc ggcgggcagc cgcgccgccg tcgtccaccg gctgctccac
     2521 gacatcggcg atgtgcgggc gcagatcgaa tttccgctgg cgcagaaacg cgcgcccttc
     2581 ctcctggcaa tcctctccga gctgacgcgc gcgttgcccg acggaacatg gctcaccgaa
     2641 ttccagctcg acgggagcaa ggttcacatc cagggcttct ccaaatcggc gtccgatgtc
     2701 gtgggcgtgg tcgaccgctc gcccatgttc gcgaacgccc agttcatggc gccgctgcag
     2761 agcgccccca acggtctcga gcgcttcgat ctctccttcg acgtcaaggg ggcgcaccgc
     2821 tgatgaccgc tcccgcgttc ctgcgcggcc gcaaaggcgc ctttcttgct gtcggcgcca
     2881 tcgttttgct gttcgccctt gtctttgcgc ttccggtcgc cgccctgttc cgcgcccagg
     2941 ccgacgaccg cgccgaagcc ctggcccagc ttgcagccta ccgcgcccag gaagcccagc
     3001 ggccggcgct cgaggcgcgt cttgcggcgc ttcagcaggg cgcgaaatcg gtccccgggc
     3061 ttctcaccgc atcaagcgcc gctctcgccc aggcgcagct gcaaagcgac atgaaggaac
     3121 tcatcgaccg cagcggcggc ggacttctga gcgcacaatt gcttccgccc accagggtca
     3181 agggattcga ttcggtggcg atcgaatatg atttgacgat tccggtgtcg cgtttcagct
     3241 cgctggttta tgcggtcgag acgcatacgc cgtattattt cgtcgacagc gccgatttcg
     3301 tgatgccgcc gaactggcgg cccaacccgc aagggcaggc gcaggatccg tcgatggaga
     3361 tgcgctggac catccatgcc ttccgctgga gaggctcgcg atgagtcttc ggctcaaccc
     3421 ggcacagatc gcgctcgccg cactttgcgg catgttgtcg atcctgctcg gctacatgct
     3481 gttcgcgccg ttgcccgaca ttccggcgcc cgcgatccag acgcatgcgg acccggtcga
     3541 cgacgcgccg cccgcgcgct tcgatgcgcc gcccaagcag gcttttgccg aggtcgacga
     3601 ccgctcggtg ttcaatccgc tgcgcgtgcg cgtgagcgga ccctccgcgc ccggcgctgc
     3661 aaacaccaac agcctgcccg ccgatctctc gcttgtcggc gtcatcctcg acggcgacac
     3721 cagactcgcc ctcttcaaat cggccgctgc gccgcttgcg gtgggcgttt cggtgggtgg
     3781 cagcatcgaa ggctggcagg tcacgcgcgt cgggcccgac gaggtgggca tgagatcggc
     3841 cgccggagaa caggcaatca agcttgccgc gggcaagccc tccggacagc cgccggggca
     3901 gccggtgggc ggacccggca acagccctct caatgcggcg caggcgaacg cccaggcgcg
     3961 gttccaggcc aacatggcgc agcgcaacaa ccccgccaac aacaacgcga acaacaacgc
     4021 gaacaacgcc aacaacaacg ccaacaatag cgacgacgac gacaattagg cgccgcaaac
     4081 gccgagcggc gggcgagatt gaatcgccac aaagaaggaa ggaagaattt agcgcagagg
     4141 gcgcagaggt tgcgcagggg tacgcagggg aaaatgttgc gcgcctctgg cgcgcgtttc
     4201 tctttcctcc gcgtcctctg cgcatcctct gcgtcctctg cgctaaaact tttcttcgtg
     4261 ctcctgttgg ggaatctcaa gcggcccggc gtgcaaactt cacgtctctg tgatgtcggc
     4321 gcccgcgtgg ggcattcctg tgataggctg acgccgcctt tgtcagggga tcgattcatg
     4381 cgcaggatgc atcgtgttgc ggtattgacg cttgcggcat gcgctgtgcc ctcggccctg
     4441 tttgcgatgg gcggcggcgg ttatggcggc gggttcagct cgatgcgcat gcccaccagg
     4501 cccgacgatt acagcgccgc gctgaagctg attcatcagg agcgatacgc cgaggcgatg
     4561 ccctgtctcg acagggcggt cgcccagcat ccgcacaatg ccgacattct caactatctc
     4621 ggctacacgc accggatggt gggggattat cccggttcgc tcgcctggta tcggaaggcc
     4681 cttaccgaag atcccgatca caagggcgcg catgaatatc tgggcgagct ctatctgaac
     4741 atgcgcgatg tcgcttcggc gcgcacgcag ctcgccgagc tcgtgcggct ttgccccgac
     4801 acttgcgacg agcgcgatgt actcaccaaa tcgatcgaca cctgggtcgc cgccaatccc
     4861 ccggcggcga tgccggccac cgccgccacc gccgcggctc ccgcatcacc ggcgacgcct
     4921 tcaacatcca cgcccccaac atcacccccc g
//
LOCUS       DASZMC010000127         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_872370,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000127 DASZMC010000000
VERSION     DASZMC010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872370"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VG274_02550"
     CDS             <1..364
                     /locus_tag="VG274_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05558.1"
                     /translation="ADAIAQKIRKARTDPAPLPDSEKGFEGRPEAENLVNIFAALADE
                     PRDATIARFAGQQFSSFKNELAELAVAKLTPIAAEMRRLLADPTEIDLLLLRGAARAR
                     ALASPVLADVKRVVGFVS"
     gene            369..1136
                     /locus_tag="VG274_02555"
     CDS             369..1136
                     /locus_tag="VG274_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE05559.1"
                     /translation="MIAAFEPKSALVTGAAKRLGRAIVLDLAKNGWDIALHFNHSEPD
                     ARETADAVRNTGRRVTLLKADLADETATSALIGRAVAEMGRLSALVNSASIFESDDWQ
                     SASQASWGRHMAVNLRAPFVLAQEFARQLRPEAHGAIVNIIDQRVLKPTPQFLSYSTS
                     KAALYWLNTTLAQALAPRIRVNAVAPGPTMINARQSEAHFRRQRDATVLGTGAEPQDV
                     CDAVRYLLSARTVTGQMIAVDGGQHLIWRTPDVSGVE"
     gene            1147..1560
                     /gene="folB"
                     /locus_tag="VG274_02560"
     CDS             1147..1560
                     /gene="folB"
                     /locus_tag="VG274_02560"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWE05560.1"
                     /translation="MTAAPSPRFADSTAALRHVFIRNLELLAHIGIHGHERTKHQPVR
                     VNVDMAVEDTTSLEDRLDRVVDYEAITLRIRALVAKGHINLAETLAESIAAACLEDAR
                     VRNVRVRVEKLHAVPGAESAGVEIERSARKLPRPD"
     gene            complement(1562..1951)
                     /locus_tag="VG274_02565"
     CDS             complement(1562..1951)
                     /locus_tag="VG274_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05561.1"
                     /translation="MIAVDTSALVAILWNEQESPRLRASLAEANGAVISAGNVLELQL
                     VLAGAHAEANWSQVEALFRAYGVEARPFDEAQLRIAREAALRFGKGRHRAGLNFGDCF
                     AYALARAEGLNLLCVGEDFALTDLELA"
     gene            complement(1948..2232)
                     /locus_tag="VG274_02570"
     CDS             complement(1948..2232)
                     /locus_tag="VG274_02570"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWE05562.1"
                     /translation="MDVSSMGALNIKDMRVAQKARRLARLKGTSITAAVSDALDESLR
                     HADTKSTVARQAREREVDAIVKRFKAGLKKGAPSPAKIMREMYDERGLPR"
     gene            complement(2316..2522)
                     /locus_tag="VG274_02575"
     CDS             complement(2316..2522)
                     /locus_tag="VG274_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05563.1"
                     /translation="MQRPVVSRDPEKTAAQFFKRRVGKARPRDLLSFLQNTPAKSPLR
                     GDEALRRPSPYEFSMAVSEVRFLP"
     gene            2636..>3082
                     /locus_tag="VG274_02580"
     CDS             2636..>3082
                     /locus_tag="VG274_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWE05564.1"
                     /translation="MTEVPSSAEADVPALAGPARIQTYLKTLPDAPGVYRMLDAKGNV
                     LYVGKAKSLKKRVASYVKTGGHIDRIARMIADTAEMLFVTTASEIEALLLESNLIKRL
                     KPRYNVSFRDDKSFPNILLREDHAFPQLLKHRGAKTTKGVYFGPFAS"
BASE COUNT          609 a         1022 c          918 g          533 t
ORIGIN      
        1 cgccgatgcg atcgcgcaga aaatccgcaa agcccgcacc gacccggcgc cgctccccga
       61 ttcggagaaa gggttcgagg gaagaccgga agccgaaaac ctcgtcaaca ttttcgcggc
      121 gctggcggac gagccgcgcg acgccacgat cgcacgcttc gcgggccagc aattctcgag
      181 tttcaagaac gaactcgcgg aactcgcggt ggcgaaactg acgccgatcg ccgccgagat
      241 gcgccgcctg cttgccgatc ccacggagat cgacttgctc ctgttgcgcg gcgccgcaag
      301 ggcccgcgcg cttgcttctc cggtcctcgc agacgtcaag cgggtcgtgg gattcgtgag
      361 ttgaaagaat gattgcggca ttcgagccaa aatccgctct cgtcaccggc gctgccaaaa
      421 ggctggggcg cgcgatcgtg ctcgacctcg cgaagaacgg ctgggacatc gcgcttcatt
      481 tcaaccattc cgaaccggat gcgcgggaaa ctgcggacgc ggttcgcaat accggccgcc
      541 gcgtgacgct cctcaaagcc gatcttgccg acgaaaccgc gacctccgct ctgatcggcc
      601 gcgccgtcgc ggagatgggg cgactgtccg ccctcgtcaa cagcgcctcg atcttcgaga
      661 gcgacgattg gcaaagcgcg tcgcaagctt cctggggccg ccacatggcg gtgaatctgc
      721 gcgcgccttt cgtgctcgcg caggaatttg cgcgccaatt gcggcctgaa gcgcacggcg
      781 cgatcgtcaa catcatcgac cagcgcgtcc tcaagccgac gccacaattt ctctcctaca
      841 gcacgagcaa ggcggcgctc tactggctga acacgacgct cgcgcaggcg ctggcgccgc
      901 gaatccgcgt caacgccgtg gcgccggggc ccacgatgat caatgcgcgg caaagcgaag
      961 cgcatttccg ccgccagcgc gacgcgactg ttctgggtac gggcgccgaa ccccaggacg
     1021 tatgcgacgc cgtacgctat cttctctccg cgcgcacggt caccggacag atgatcgcgg
     1081 tggacggcgg ccagcacctg atctggcgaa cccccgacgt gagcggcgtg gaatgagagc
     1141 aaccgcatga ccgccgcacc ttcgccccgt ttcgccgatt ccacggccgc gctccgccac
     1201 gtgttcatcc gcaatctcga actgctcgcc catatcggaa ttcacggcca cgaacgcacc
     1261 aagcaccagc ccgtccgcgt caatgtcgac atggcggttg aggacaccac gtcgctcgaa
     1321 gaccggctcg accgtgtggt cgactacgag gcgatcaccc tgcgcatccg cgctctcgtc
     1381 gcgaaggggc atatcaacct cgctgaaaca ctggcagaat ccattgctgc cgcgtgcctg
     1441 gaagatgcgc gcgtgagaaa tgtgcgcgtg cgggtcgaga agctccacgc cgtccccggc
     1501 gccgaatctg ccggcgtcga aatcgagcgg agtgcccgca aactcccgcg acctgactga
     1561 ttcaggcgag ttcgaggtcc gtgagagcaa aatcttcacc gacgcaaagc agattcaatc
     1621 cctcggcgcg cgccagggca taggcgaagc agtcgccgaa gttgagtcct gcccgatggc
     1681 gaccctttcc gaagcgcaac gcggcttcgc gggcgatgcg aagctgcgcc tcgtcgaatg
     1741 gccgtgcctc taccccatag gctctgaaga gcgcttccac ctggctccag tttgcctcgg
     1801 catgcgctcc ggcaaggacg agttgaagct ccagcacatt gcctgcggaa atcacagcgc
     1861 cgttcgcctc cgcaagactt gcacgcagcc tgggggactc ctgctcgttc cagagaatgg
     1921 cgacaagtgc agacgtatcg acggcaatca acgcggcaga ccgcgctcgt catacatttc
     1981 gcgcatgatc tttgcaggag agggggcgcc cttcttcagg ccggctttga agcgcttgac
     2041 gatcgcatcc acctcacgct cgcgcgcctg acgcgcgact gtcgatttcg tatcggcgtg
     2101 ccgcagactc tcatcaagcg cgtccgaaac cgcggcggtg atgctcgtgc ccttcaaccg
     2161 ggccagccgt cgcgcctttt gggcaactcg catgtctttg atgttgaggg cgcccatgga
     2221 agaaacatcc atattttctt cctcggcaat agcacgagca atacttcacg gcaagcggtc
     2281 gaaacgaggt cgagtattcg agggcagcgt tgtcttcacg gcaagaagcg aacttccgac
     2341 accgccatcg aaaactcgta tggcgacggg cgcctcagcg cctcatcgcc gcgaagcggc
     2401 gatttcgccg gagtattctg gaggaacgac aacaggtcgc gcggtctggc tttgccgaca
     2461 cggcgtttga agaactgagc cgcggtcttc tcgggatcgc gcgaaacgac tggccgttgc
     2521 atgagcgcaa gttatcgccg ggaaagccat cgcgcaacgc agttctgatg cgcgttcggc
     2581 gacgggaatt caggcgttat gagcggattg ccgcgcgcaa tgctatattg cgctcatgac
     2641 cgaggtgccg tcatcggctg aagcggacgt gcccgcactt gcgggccccg cgcgcatcca
     2701 gacctatctg aagacgctgc cggatgcgcc gggcgtctat cgcatgctcg atgcgaaggg
     2761 caacgtgctc tatgtcggca aggcgaagag cctgaagaag cgtgtcgcct cctacgtgaa
     2821 aaccggcggc catatcgacc gcatcgcgcg catgatcgcc gacacggccg aaatgctgtt
     2881 cgtcaccacg gcatcggaga tcgaggcgct gctcctcgaa tcaaacctga tcaaacgcct
     2941 caagccgcgc tacaacgtct cgttccgtga cgacaaatcc tttcccaaca tcctcctgcg
     3001 cgaagaccac gctttccccc agttgctcaa acatcgcggc gcgaaaacca cgaaaggcgt
     3061 ctatttcggt ccatttgcct cg
//
LOCUS       DASZMC010000128         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_613326,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000128 DASZMC010000000
VERSION     DASZMC010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613326"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VG274_02585"
     CDS             complement(<1..703)
                     /locus_tag="VG274_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05565.1"
                     /translation="MNTETRPFTDWALPLAILAMAVAFIIVDPAHLALRLANFQLDAW
                     RAIEARRATPITPAFLMTQAAFLAPAGATLTLLLARGRLFWAGAFTVTMIAMMQGASW
                     IAYASGRTLFDTANASAALLLAAGAGYCAFAITGRREAAIEPRTFLIGGGEEPAGEPS
                     TAPGESRAITTLACGLRGVSQLAESYSGDIADLTRVVNAVMAPLVDDAAQHGAVIGHF
                     DGTTFSAHWTAPLEGT"
     gene            complement(785..1507)
                     /locus_tag="VG274_02590"
     CDS             complement(785..1507)
                     /locus_tag="VG274_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWE05566.1"
                     /translation="MFIALGACGATLAGGALALRLRDRLHLILAFSAGAIIALAFFDL
                     LPEAIKIGSSTFEPASVLAIAAVGFFGYTVLDRMILLHAHHDGGPGPAEGALAERQWA
                     GAGSLSVHSLMDGFAIGVAFRASPGIGIVVAAAVLAHDCSDGMNTVNLVLKNGGSTKQ
                     AFRWLFTDALAPVVGAAASLMVSLPSSAISSLLGLFAGFFLYIGASDLLPESFHAHPK
                     FLTTLMTLFGAGLLYVVARLAG"
     gene            1656..2771
                     /locus_tag="VG274_02595"
     CDS             1656..2771
                     /locus_tag="VG274_02595"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWE05567.1"
                     /translation="MHDITKDPAYNAVDPGDFPAMLEIERYGNRSDAFDRIISATHDH
                     FWDPLDSKYIDFSQRFDTANEYLVNPSANTDLKTAIGDRLDESRKIKLVNLDVWWSFS
                     SILHGEQGALSLSASLCHILRDPGAQEYAANQTREEARHVTAFTRYIQARWGKPVSVG
                     PSLGNLLEELVASPLVWKKLVGMQMLVEGLAMGAFAMFFKEARDPLMRQVCQLVMTDE
                     AFHHKFGKIWAERTIPHISREEHEMIEDWAAQVFQILLFNLGSPDQKPWIYEAVGLDP
                     AWCQSAFMEGLTDTLIREELRDAANLFRVLIKTLLKAGIITDRTRGIYAAYVDMIELY
                     DEGDRMVGDDIAEEGIKHLLKLNAGSRGRVQPSLAAE"
     gene            2781..>3150
                     /locus_tag="VG274_02600"
     CDS             2781..>3150
                     /locus_tag="VG274_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE05568.1"
                     /translation="MPGRVEGKLALVTGAAQGLGEAAARMLVREGGRVAVTDINEDGA
                     RRVAHSLNALRANSAIAISHDVTHEEQWRHAIDETERHFGGLHVLVNNAGIGLTKDFE
                     TVTLEEWRRVHAIDLDGVFLG"
BASE COUNT          632 a         1019 c          979 g          520 t
ORIGIN      
        1 ccgttccctc cagaggcgcg gtccagtgcg cggaaaaggt cgtgccgtcg aaatggccga
       61 tcaccgcgcc atgctgggcc gcgtcgtcga cgagcggcgc catcaccgca ttcacgacgc
      121 gcgtcaaatc cgcgatatcg cccgaataag actctgcgag ctgcgagacg ccgcgcaggc
      181 cgcaggcgag cgtcgttatc gcccgcgact cgccgggcgc ggtcgagggc tcgcccgcgg
      241 gctcctcgcc tccgccgatc aagaacgtcc ttggctcgat ggccgcttcg cgccggccgg
      301 tgatggcaaa ggcgcagtag ccggcgccgg cagcgagcag aagcgccgcg ctcgcatttg
      361 cggtatcgaa aagcgtccgc ccgctcgcgt atgcgatcca gctcgcgccc tgcatcatcg
      421 caatcatcgt cacggtgaat gcacctgccc agaacagacg gccgcgcgcc aggagcaatg
      481 tcagggttgc tcccgccggc gccagaaacg cggcctgggt catcaggaaa gcgggggtta
      541 tcggcgtggc gcgccgcgcc tcgatcgccc gccaggcatc aagctggaag ttcgcgagac
      601 gcagtgcgag atgcgccgga tcgacgatga tgaatgcaac cgccatcgcc agaatggcga
      661 ggggcaatgc ccagtcggtg aacggcctcg tttcagtgtt catcgcgaat gcacgggccg
      721 tgaatcatcc tgtccccatc gtgctcttct atcgcgcaaa agggcaggaa ttgaaagatg
      781 cgcgctaccc cgcaaggcgt gcaacgacgt agaggagacc ggcgccgaaa agcgtcatca
      841 gcgtggtcag gaatttcgga tgcgcgtgaa agctttcggg gagcaggtcg ctcgctccga
      901 tatagagaaa gaagccggcg aacaatccca gcagggacga tatggcggag ctgggcagag
      961 aaaccatcag actcgccgcc gcgccgacga ccggcgcaag cgcatcggtg aaaagccagc
     1021 ggaatgcctg tttcgtgctg ccgccgttct tcagtacgag attgacggtg ttcatcccgt
     1081 ccgagcaatc atgcgccagc acggccgccg ccaccacaat gccgattccg ggcgaggcgc
     1141 gaaaggcgac gccgatcgcg aagccatcca tgagactgtg cacggaaaga cttcccgcgc
     1201 ctgcccattg gcgttcggcg agcgcgcctt cggccgggcc ggggccgccg tcatgatgcg
     1261 catgcaagag gatcatccgg tcgagaacgg tatagccaaa aaaacccacc gccgcgatgg
     1321 cgagcacgga cgcgggttcg aatgtcgagc tgccgatctt tatcgcttcg ggcaacagat
     1381 cgaaaaacgc cagcgcaatg atcgccccgg cgctgaaggc gagaatcagg tgcagccggt
     1441 cgcgcaggcg taacgccagt gcgccgccgg ccagagtcgc gccgcaggcg ccgagcgcga
     1501 tgaacatgat cagtgcttgc tgcaaaccgg gtccccgctg ccagacgcac ccgacgatca
     1561 ccgctgcgcg acgccttcgt caacttcctt gccgccatcc cgcgaccggc ttaggattcc
     1621 gttccggaat gcggctttcc tgcgaggaac ggctcatgca cgatattacc aaggatccgg
     1681 cctacaacgc cgtcgatccg ggcgactttc ccgccatgct cgagatcgaa cgctacggaa
     1741 accgatcgga cgccttcgac cgcattatct ctgccaccca cgatcatttc tgggacccgc
     1801 tcgacagcaa atatatcgat ttctcgcaga ggttcgatac cgcgaacgaa tatctcgtca
     1861 atccctccgc caacaccgac ctgaagacgg ccatcggcga ccggctcgat gaaagccgga
     1921 aaatcaagct cgtcaatctc gatgtctggt ggtcgttctc ctcgatcctg catggcgagc
     1981 agggcgcgtt gtcgctttcg gcttcgctct gccacatcct tcgcgatccg ggcgcgcagg
     2041 aatacgccgc gaaccagacg cgcgaggagg cccgccacgt cacggcgttc acccgctata
     2101 tccaggcgcg ctggggaaag cccgtttccg taggcccctc tctcggcaat ctgctcgagg
     2161 aactcgttgc ctcgccgctg gtgtggaaga agctcgtcgg catgcagatg ctggtggaag
     2221 gcctcgccat gggcgccttc gccatgttct tcaaggaagc ccgcgatccg ctgatgcggc
     2281 aggtctgcca gctcgtgatg actgacgaag cgttccacca caaattcgga aagatctggg
     2341 ccgagcgcac cattcctcac atttcgcgtg aggagcacga gatgatcgag gattgggcgg
     2401 cgcaagtgtt ccagatcctg ttgttcaatc tcggcagtcc cgaccagaag ccctggatct
     2461 acgaggccgt cggcctcgat cccgcctggt gccaaagcgc cttcatggag ggactgaccg
     2521 acacactgat ccgcgaagaa cttcgcgatg ccgccaatct ctttcgcgtg ctcatcaaga
     2581 cattgctcaa ggccggcatc atcaccgacc ggacgcgcgg catctatgcc gcctatgtcg
     2641 atatgatcga gctctacgac gagggcgacc gcatggtggg cgacgatatc gcggaagagg
     2701 ggatcaaaca tctcctcaag ctgaacgccg gaagtcgcgg ccgcgtgcag ccatcgctcg
     2761 cggcggaata gagcgggcga atgcccggac gcgttgaagg aaagctcgca ctcgtcaccg
     2821 gcgccgccca agggctcggc gaagcggcgg ctcgcatgct cgtgcgcgaa ggcgggcgcg
     2881 tggccgtgac cgacatcaat gaggacggcg ccaggcgtgt ggcgcattcg ctcaatgcgc
     2941 tgcgcgcgaa ttccgccatc gcgatctccc acgacgtgac gcacgaagag caatggcggc
     3001 atgcgatcga cgaaacggag cggcatttcg gcggcctcca tgtgctcgtc aacaatgcgg
     3061 gcatcgggct caccaaggat ttcgaaacgg ttacgctcga ggaatggcgg cgcgtgcacg
     3121 ccatcgacct cgacggcgtt ttcctcggat
//
LOCUS       DASZMC010000129         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1972145,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000129 DASZMC010000000
VERSION     DASZMC010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972145"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VG274_02605"
     CDS             complement(<1..244)
                     /locus_tag="VG274_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05569.1"
                     /translation="MTTKAAPAPTKVVAKSAGVSKTAVKSTAAAKAGTTAAASSKGIA
                     KKAVAKRAATPRYTAQQQPTSDRYRDIQQALADRGYF"
     gene            237..530
                     /locus_tag="VG274_02610"
     CDS             237..530
                     /locus_tag="VG274_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05570.1"
                     /translation="MVIDFAAVHFAAAAFAAVVFAAVAFVVADFDWPEGAAVFDVVFL
                     DVDRVLAVEDCAAIRGSRAMIVAAIAAAAIHTARLITRGVCNAGTLRTPLPGT"
     gene            complement(527..2113)
                     /locus_tag="VG274_02615"
     CDS             complement(527..2113)
                     /locus_tag="VG274_02615"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWE05571.1"
                     /translation="MKLLEKMRQQKLLSMTLVLVTLSVGIVIGTLINTGVHAQRGQAA
                     APDATPLTIPKGTVAGNEITKLAKTLEPSVVNITADFTPKVSTKKSDPDDDQSQDPSD
                     LFRRFFGGQGGGDLPSPNQRREQSGTGFIVDKNGYIVTNNHVVDGVDRIRVKLHGDDV
                     EYRARLIGTDRETDVAVIKIDAHRPLTPVTIANSDTVQVGEEAVAIGSPFGLEATVTA
                     GIVSATGRDIGAQQFQRFIQTDAAINPGNSGGPLLNIRGEVIGVNTMIATQSGGYQGV
                     GFALPINTVVRVYNDIIKEGRVTRGSIGVSWDKNTKMETLRALGVDHGVIVGDVRKDG
                     PADKAGVKATDIILALNGQNVKDGDDLVARVADMPVGSQATLTVNRDGKKMDLKLTIL
                     DRTKVFSDDPRVVGENGLPSGGGGGDKPEASQVRFGISIRPASDEEKDTTPDKKGVTV
                     TRVEPGSFAEDIGMMEHDVIIAINRDSIGSVDDIRRIQQGMKAGDPVAFRVVRTPQGA
                     RRNNGSTPHVTMFLSGTLPN"
     gene            complement(2322..>3260)
                     /locus_tag="VG274_02620"
     CDS             complement(2322..>3260)
                     /locus_tag="VG274_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWE05572.1"
                     /translation="LDTVFAPRTPDDIKVAPDGRFLGPGVSDNGAGLAALLAIAAAWN
                     AAPPLESPSAWPLLVANVGEEGEGNLNGMRYFCRHANTSKLKALLVLDGPDAEHITSR
                     ALASRRFEVIITGPGGHSWRDFGVPNPVHAVARAITLFTDQAVEETFGDPRTTFNFGI
                     VEGGSSINSIPSEARTKIDLRSEDNTRIDRLAQRLTAAVERAVESENALSNSGRATAR
                     VREIGSRPSGALADDAPLLECVRGVDAHLGIRSYLECSSTDANVPLSLGLPALSIGAG
                     GLGSGAHTAGEWYSPEGREHGLQRIVLILCMMLMGE"
BASE COUNT          570 a         1043 c          976 g          671 t
ORIGIN      
        1 tgaaatagcc gcgatcggcc agcgcctgct gaatgtcacg gtaacgatcg gacgtgggct
       61 gttgctgcgc ggtgtagcgc ggagtggccg cgcgctttgc gacggccttt ttcgcgatgc
      121 ccttcgaaga cgcagccgcc gtggttccag ctttcgcagc agccgtgctc ttgacggcgg
      181 ttttggaaac gccagcggac ttcgcgacta ctttcgtcgg cgccggtgct gccttcgtgg
      241 tcatcgattt cgcagccgtc catttcgcag ccgctgcttt cgcggcggtt gttttcgcgg
      301 cagttgcttt cgttgtcgcc gatttcgact ggccggaggg cgctgcggtt ttcgacgtgg
      361 tttttttgga cgtcgaccgt gtgctcgccg tggaggattg cgcggccatc agaggcagcc
      421 gtgccatgat tgtcgcggcg attgccgcag ctgcgatcca caccgctcgc cttattacgc
      481 gcggcgtttg taatgcgggc acgttgcgga cgcctttacc cggaacctag ttgggcagcg
      541 tcccgctgag gaacatggtg acgtgcggcg tggagccgtt attgcgacgc gcgccctgcg
      601 gcgtgcgaac tacgcggaac gccaccgggt cgccggcctt cataccttgc tggatgcggc
      661 ggatatcatc caccgatcca atgctatcgc ggttgattgc gatgatgacg tcgtgctcca
      721 tcatgccgat atcttcggcg aaggagcccg gctccacgcg cgtcacggtg acgcctttct
      781 tatccggcgt ggtgtccttt tcttcatcgc ttgcagggcg aatgctgata ccgaagcgca
      841 cttgcgatgc ctcgggcttg tccccgccgc cgccaccgct gggaagtccg ttctctccca
      901 ccacgcgcgg atcgtccgag aagaccttgg tgcgatccag aatggtgagc ttcaggtcca
      961 tctttttgcc gtcgcggttc acggtgagcg tagcctgcga accgacaggc atatccgcca
     1021 cgcgcgccac cagatcgtcg ccgtccttca cgttctgccc gttgagggcc agaataatat
     1081 cggtggcctt cacgccggcc ttatccgcgg gaccgtcctt gcggacgtcg cccacaatca
     1141 caccgtgatc cacgcccagc gcgcgcaacg tttccatctt cgtgttctta tcccatgaca
     1201 ctccgatgga gccgcgcgtg acgcgccctt ccttaatgat gtcgttatac acgcgcacca
     1261 ccgtgttgat gggcagcgcg aagcccacgc cctgatagcc gccgctttgc gtggcgatca
     1321 tcgtgttgac gccgatcact tcgccgcgaa tgttcagcag cgggccgccg ctgttgccag
     1381 gattgatggc cgcgtcggtc tgaatgaagc gctggaactg ttgcgccccg atgtcgcgcc
     1441 ccgtggcgct gacaatgccc gcggtgaccg tcgcttccag accaaatgga gaaccgatgg
     1501 cgaccgcttc ttcgcccact tgcaccgtat cggaattggc gatggtcaca ggcgttaaag
     1561 gccggtgcgc gtcgatcttg atcaccgcga cgtcggtttc gcggtctgtg ccgatcagcc
     1621 gtgcgcggta ttccacatcg tcgccgtgca gcttcacacg aatgcggtcc acgccgtcca
     1681 ccacgtgatt gttggtcaca atgtagccgt tcttatccac gatgaaaccc gtgcccgact
     1741 gctcccgccg ctggttgggc gaaggaagat cgccaccgcc ctggcctccg aaaaagcggc
     1801 gaaacagatc gctgggatcc tgcgattggt cgtcatcggg atcgctcttt ttcgtcgaga
     1861 ccttcggtgt gaaatcggcc gtgatattga ccacgctcgg ctccagcgtc ttggccagct
     1921 tggtaatttc gttacccgcc acggtgccct tgggtatggt cagcggcgtc gcatcgggag
     1981 cggcagcctg cccgcgctgc gcatgcacac ccgtgttgat cagcgtaccg atcacaatgc
     2041 cgaccgacag cgtgacaagc acgagtgtca tggacaatag cttttgctgg cgcatttttt
     2101 ctaacagttt catctcaagc aacttcctca taggagctcg taagtgccaa aaactctgtg
     2161 aaaagagact ccttcaacat tatatattta cagacgccgg aaggcacgag cgggttgcca
     2221 tgccgcgcgc ggattatgag gtttatctgg agtacaagcc gaaagattcg aacgcaagat
     2281 ccggacgaag acgatggtga aagaaatccc gcgctcgcgg actactcccc catcagcatc
     2341 atgcaaagaa tcagcacgat ccgttgcaag ccgtgttccc tgccctccgg cgaataccac
     2401 tcgcccgccg tgtgagcgcc gctgccgagc ccgccggcgc cgatcgacaa cgccggaagc
     2461 cccagcgata aagggacgtt ggcatcggtg gaagaacatt ccagatagct gcggatgccc
     2521 aggtgcgcgt ccacgccgcg cacgcattcc agcaacggag cgtcatcggc cagcgcgccg
     2581 ctggggcgtg agccgatctc gcggacgcga gccgtcgcac gcccgctgtt gctcagcgcg
     2641 ttctccgact caaccgcgcg ctccacggcc gccgtcagcc gctgcgccaa acgatcgatg
     2701 cgcgtgttgt cctccgaccg tagatcgatc ttcgtgcggg cctcgctggg aatcgaattg
     2761 atgctggatc cgccttcgac gatgccgaaa ttgaacgtgg tgcgcggatc gccgaaggtt
     2821 tcttccacgg cctgatcggt gaataacgtg atggcgcgcg ccacggcatg caccggattc
     2881 ggcacgccga aatcacgcca actgtgtccg ccgggtcctg tgatgatcac ctcaaagcgg
     2941 cggctggcca gcgcgcgaga tgtaatatgt tccgcgtccg ggccatccag caccagcagc
     3001 gctttcaatt tcgaggtatt cgcatggcgg cagaaatagc gcatgccatt cagattgccc
     3061 tcgccctctt cgcccacgtt ggccaccaac aacggccacg ccgaaggcga ttccagcggc
     3121 ggagcggcgt tccaggccgc ggcgatcgcc agcagcgcgg ccagccctgc gccgttatcg
     3181 gaaacgccgg ggcccagaaa gcggccatcg ggcgcgacct tgatatcgtc cggcgtgcgc
     3241 ggagcgaaca ccgtatccag
//
LOCUS       DASZMC010000130         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1162992,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000130 DASZMC010000000
VERSION     DASZMC010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162992"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2327
                     /locus_tag="VG274_02625"
     CDS             <1..2327
                     /locus_tag="VG274_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05573.1"
                     /translation="VIEKLRQMDAATLRTYLGGSNTFASRQKIVTTYLDSTPLGSRPG
                     YGEIIGIGDGLRVWYGTDFAEANRILADSNSSDLSRKAEIYKQVLSLLLAERRPAFYL
                     TADHRALLALTNNYLRRLAAQKAIDSSLFNAASQAPLPFRAQAPAPAPVSFIGRKGAD
                     AIRTELLGLIGARSLYDLDHVDLGARSTIDAAAQRRVTDLLARLRDPDQVTALGLEGK
                     DLLGGGDPALVNYSVVLYEHTAGHNLVRVHADSMDQPFDLNSGTKLQLGSTAKLRTLI
                     SYLDIISGLHDRYAELPARKLNALMPQARDPLSQWALGYLAGARDRRLQPMLDAAMQR
                     HYSAGVGGFFTGGGFHAFHNFAKWENHGNPTVEIAFANSINLSFVRIMRDIERYYVAQ
                     DHLQAGALDDEPSGDARTAFLQRFADQEGRVFLDKFVREFRGLGSDQRLSLLARKTGP
                     YAKRLTVVFRSVHPRASVADLAAFLKREVKGKLDLKQPLEKLYAAYGVDRFSLADRGY
                     LSHVHPLELWLASWLEQHPQATRHEIMQAGAPVRQEVYGWLFKTRNMHKQNWRIRVLV
                     EQDAFKRITADWRRLGYPFAHLVPSLATAIGSSGDRPDALAELMGIILNNGVRQPTID
                     IRDLQFGAGTPYETDMSAGHDKAVRVLPAEVAATVRRALSDVVLEGTGKRIQSAYHAA
                     DGRPLVLGGKTGTGDNRFDSFSAGHALIESRVVDRTSTFVFYLGDRFFGTITAYVPGR
                     KAAQFHFTSALAVQLLKTMSPQIQPLFERPTANS"
     gene            complement(2372..>3053)
                     /locus_tag="VG274_02630"
     CDS             complement(2372..>3053)
                     /locus_tag="VG274_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWE05574.1"
                     /translation="HLRPHRSERELRTQLGGFGLSGDKALTRVGLLSGGERARLMLTL
                     ATLDSPNLLILDEPTNHLDIDAREQLLVALNDFDGAVVLVSHDRRLIEATIDRLLLVA
                     NGTVRTFDGDLDDYRRQILSPAKPAREKRNPVRKEDTRRSAAERRAQLKPLREEAIAA
                     EQEVARLTRDVAEYDAALSAPTLHKREPSRASELSRQRTHAMRALTDAETHWLAASQA
                     YEDASAKE"
BASE COUNT          535 a          985 c          937 g          596 t
ORIGIN      
        1 gcgtgataga aaagcttcga cagatggacg cggcgacctt gcgcacctat ctgggtggct
       61 cgaacacgtt tgcgtcgcga cagaagatcg taaccaccta tctggattcc acgccgctcg
      121 gttcgcggcc gggatacggc gaaatcatcg gcatcggaga tggattgcgg gtctggtacg
      181 ggactgactt cgccgaagca aaccggattt tggcggattc gaattccagc gatctttcgc
      241 gcaaggcgga aatctacaag caggtcctga gcctgcttct ggccgagcgc cgtccggcct
      301 tttatctgac tgccgatcac cgcgcgcttc tcgctctgac gaacaactat ctgcgccggc
      361 tggcggcgca aaaggctatc gacagcagtc tgttcaatgc cgcgtcgcag gcgccgctcc
      421 ccttccgcgc ccaggcccct gcacccgcgc ctgtgtcctt tatcgggcgc aagggcgcag
      481 acgcgatccg caccgagctg ctcggcctca ttggcgcacg cagcctctac gatctcgatc
      541 acgtcgatct cggagcaagg agcaccatcg acgccgcggc gcaacgtcgc gtgaccgacc
      601 tcctcgcccg attgcgcgat cccgatcagg tcacagcgtt gggcctggaa ggcaaggatc
      661 ttctcggcgg cggcgatcct gcactcgtca actatagcgt ggtcctctac gaacatacgg
      721 ccggccacaa tctcgtgcgc gtccatgccg acagcatgga ccagccattc gacctgaact
      781 cgggcacgaa actgcagctc ggctcgaccg cgaagctgcg cacgctcatc tcctatctcg
      841 atatcatctc cggtctccac gaccgctatg cggagcttcc ggcgcgcaag ttgaatgcgc
      901 tgatgccgca ggcgcgcgat cccctgtcgc aatgggcgct tggttatctc gcgggtgcgc
      961 gggatcgccg cctgcagccc atgctcgacg cggctatgca acgtcactat tccgccggcg
     1021 tcgggggctt cttcaccggc ggaggctttc acgctttcca caatttcgcg aaatgggaga
     1081 accacggcaa tccgacggtg gaaattgcat ttgcgaattc catcaatctc tccttcgtgc
     1141 gcattatgcg cgacatcgag cgctactatg tggctcagga tcatctgcaa gccggcgcgc
     1201 ttgacgacga accgagcggc gatgcccgga cggcgtttct ccagcgcttt gccgatcagg
     1261 aagggcgcgt cttcctcgat aaattcgtcc gcgaattccg cggcctcggc tccgatcaga
     1321 gactttcgct attggcccgg aagaccggac cctatgccaa gcgtctgacc gtggttttcc
     1381 gctctgtgca tccgcgcgcg agcgtggcag accttgccgc ctttctgaaa cgcgaggtga
     1441 agggcaagct ggatctcaaa cagccgctcg aaaaacttta tgcggcgtac ggcgttgacc
     1501 gtttctcgct cgccgacaga ggctatctga gccacgtcca cccgctcgag ctgtggctcg
     1561 cgtcctggct cgagcagcac ccgcaggcca cgcgccacga gatcatgcag gcaggtgcgc
     1621 cggtgcgtca ggaagtctat ggctggctat tcaagacgcg caacatgcac aagcagaact
     1681 ggcgcattcg cgtgctggtc gagcaggatg cgttcaagcg cattaccgcg gactggcgca
     1741 ggctcggcta tccgtttgcc catctggtgc cgtcgctcgc cacagcgatc ggcagctcgg
     1801 gtgaccgtcc cgacgcgctt gccgagttga tgggcatcat cctcaacaat ggcgtccggc
     1861 agccgaccat cgatatccgg gatctgcaat ttggcgccgg cacgccctac gagacggata
     1921 tgtctgcagg gcacgacaaa gctgtccgcg tgctgcctgc ggaagttgcg gcgacggtgc
     1981 gccgcgcgct gagcgatgtc gttctcgaag gaacaggcaa gcgcatccag agcgcctatc
     2041 atgcggcgga cggaaggccg ctcgttctgg gtggcaagac cggcacgggc gacaaccgct
     2101 tcgacagttt cagcgccggt catgcgctca tcgagtcgcg ggtcgtcgac cgcacttcca
     2161 ccttcgtgtt ctatctgggg gaccgcttct tcggcaccat caccgcctat gtgccgggcc
     2221 gcaaggccgc gcaattccac tttaccagcg ctcttgcggt gcagctgctg aagacgatgt
     2281 cgccgcagat ccagcccttg ttcgagcggc cgactgcgaa cagctgatgc gctgatactc
     2341 gcaacaatcg caacgggcgg gaacacaggt gttattcttt cgccgatgca tcctcatatg
     2401 cctgcgaggc ggccagccaa tgcgtttcgg catcggtgag cgcgcgcatg gcgtgagttc
     2461 gctgacggga gagttccgac gctcgtgacg gttcgcgctt gtgcagggtc ggcgccgaca
     2521 acgctgcatc gtattccgcg acgtcgcgcg tgagccgtgc gacctcctgt tcggcagcga
     2581 tagcttcctc tctgaggggt tttagctggg cgcggcgttc ggccgcactg cgccgtgtgt
     2641 cctccttgcg cacgggattc cgcttttcgc gcgcgggttt tgccggcgag aggatttggc
     2701 ggcgatagtc gtccagatcg ccatcgaagg ttcgcaccgt accgttcgcg accagcagga
     2761 ggcggtcgat cgtcgcttcg atgagcctgc ggtcgtggct gaccagcacc acggccccgt
     2821 cgaaatcgtt cagggccaca agcagctgtt cgcgcgcgtc gatgtccaga tgattggtcg
     2881 gttcgtccag gatgagcaga ttgggcgagt cgagcgttgc cagcgtgagc atcagcctcg
     2941 cgcgctcgcc gcccgaaagc aggcccaccc tggtcagcgc cttgtcgccg gaaagcccga
     3001 agccgcccag ctgcgtacgc aattcacgct cggatcggtg cggacgcaga tgc
//
LOCUS       DASZMC010000131         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4451470,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000131 DASZMC010000000
VERSION     DASZMC010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4451470"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1670
                     /locus_tag="VG274_02635"
     CDS             23..>1670
                     /locus_tag="VG274_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HWE05575.1"
                     /translation="MKTIVFLGPTLPVSVARQYLDATFLPPVEQGDVLRALKDRPDAI
                     GIIDGYFQRVPSVWHKEILTAIAEGVHVAGAASMGALRAAELAAFGMVGIGSIFSKFH
                     SGEYTDDDEVAVAHASAELGYRALSEAMVNIRACCAEAIEAGVVSSADAQIVVKVAKA
                     LHFTGRHWEAILAGSIAAGMDPTAAQDLRLFRTRMPQGAKERDAVALLQHLATLDTQA
                     CERPRTPLRVHQTAFLADLKQQINRETVPDPEEAPPTSLNVARKKVLLGALANREAVR
                     RGLMIGHDDVNEMTGWFRGAYSLEDDDRFVTWKANHALAEPDFAFAMRRFTSVVRVEE
                     ACRPEIDAELDTYLKVYSAAAQNHSEAPVWVQLNIGLGRGAEGAQASARALFGELMPV
                     MRRLNRRGAVDCFHFVRKAPDLRLRFRAVAPDETLLPRLAKLFQKLCADGVIASVARS
                     VYEPEARVFGGAAAMDAVHDYFDADSRGWILWNETVATGHRVAAEQMCTTVIDDLFHS
                     VLGCPSEVWDAWNNLCHLTREAKVAAAKLNNAVPAGADPNV"
BASE COUNT          304 a          501 c          546 g          319 t
ORIGIN      
        1 ttgcagcatg aacgcttgca gcatgaagac cattgttttt ctgggtccga ctttgcccgt
       61 aagcgtcgcg cggcaatatc tcgacgcgac cttcctcccg ccggtcgagc aaggcgacgt
      121 tctgcgagcg ctcaaagacc gcccggacgc catcgggatc atcgacggct acttccagcg
      181 cgtgccttct gtctggcaca aagaaattct cacggcaatc gcggagggcg tgcacgtcgc
      241 gggcgcggcc agcatgggag cactgcgcgc ggcggagctg gcggcgttcg gcatggtcgg
      301 catcggctcg atcttctcga aatttcattc cggtgaatac accgacgatg atgaggtcgc
      361 agtcgcgcac gcctcggccg aactcgggta tcgcgcgctt tccgaagcga tggtgaatat
      421 tcgcgcgtgc tgcgcggagg cgattgaggc cggtgtggtc tcgtccgccg atgcgcaaat
      481 cgtggtcaag gtcgctaaag cgctccactt cactggacgg cattgggaag ccatccttgc
      541 cggttccatc gcggcgggta tggatccgac agcggcgcaa gacctcaggt tattccgcac
      601 tcgaatgcca cagggtgcaa aagagcgtga tgccgtggcg ttgctgcagc atcttgccac
      661 gctggacacg caggcatgcg aacgcccgag gacgcctctg cgcgtccacc agaccgcatt
      721 tctggcggat cttaagcagc agatcaaccg cgaaacggtt cccgatccgg aagaggcgcc
      781 tcccacgtcg ctgaacgttg cgcgcaagaa ggtgctgctt ggggcgctcg cgaatcgcga
      841 agcggttcgc cgcggactca tgatcgggca cgacgatgtc aacgaaatga ccggatggtt
      901 ccgaggcgcc tattcgctcg aggatgacga tcgctttgtc acatggaagg cgaaccatgc
      961 gctggcggaa ccggacttcg ccttcgccat gcggcgattc acgagcgtgg tgcgcgtgga
     1021 agaagcctgc cggccggaga tcgacgcgga gcttgacacc tacctgaagg tctattccgc
     1081 ggccgcacag aaccatagcg aagcaccggt gtgggtgcag ttgaatatcg gtctcggccg
     1141 cggcgcggag ggcgcgcagg cgtcggctcg ggctttgttc ggcgaactga tgccggtgat
     1201 gcggcgcttg aaccgccgcg gcgcggtgga ctgtttccat ttcgtgcgca aggcgccgga
     1261 tctcagactg cgcttccgcg ctgtcgcgcc cgacgagact ctgctgccgc gcctcgcgaa
     1321 gctttttcag aagctctgcg cagacggcgt gattgcatcg gttgcccgca gcgtttatga
     1381 gcctgaggcc cgcgtcttcg gcggggccgc ggccatggat gcggtccacg actatttcga
     1441 tgccgatagc cggggctgga ttttatggaa cgagactgtc gctaccgggc atcgagtagc
     1501 cgcggagcaa atgtgcacca ccgtgatcga cgatcttttc cattccgtcc tcggctgccc
     1561 gagcgaagtg tgggatgcct ggaacaatct gtgccatctc acgcgcgagg ccaaagtcgc
     1621 ggcagcgaaa ttgaacaatg cggttccggc aggcgcggat ccgaatgtca
//
LOCUS       DASZMC010000132         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4451784,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000132 DASZMC010000000
VERSION     DASZMC010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4451784"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1005
                     /locus_tag="VG274_02640"
     CDS             40..1005
                     /locus_tag="VG274_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWE05576.1"
                     /translation="MTRYLAEEGDVSMRGRSPAELVMGSVQLGLAYGAANRTGKPSHE
                     TALRLVHQAVAGGISQFDTARAYGDSEDRLGEALADRKDVRTITKLSALSELAPNASR
                     DEVHAAVDASIDESLNALRRGRLDCLLLHRAEHMHAFGGAVWQRLIERLEDGSVLSLG
                     VSVQNPLEALAALACPDVVHLQLPFNLLDWRWRKAGFKTCLASRHNVTVHARSTYLQG
                     ILASNDVRVWPRIDGVYPQALINIIAGLAHQLGREGAADLCLAYARGQDWIDGVVVGL
                     ETEEQLDDNLRLFIKRPLSADECALVEARIPHLPIALLNPAAWPK"
     gene            1002..1787
                     /locus_tag="VG274_02645"
     CDS             1002..1787
                     /locus_tag="VG274_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE05577.1"
                     /translation="MSELFRLDGCVAFVSGAAGHLGREMTNALCEQGAHVIVNGRDDA
                     RLAAFESELLGKGFSVERVVFDVGDATSVREFFSKLSRLDVLVNNAISMTPKPFAQLE
                     PADFEKTYASSVTAAFESVRAARPALAAAAEATGDASVVNIASMYASVSPDSRIYAKP
                     EQASPFHYGPAKAALLQLTRHLAAELGDQRIRVNALVPGPFPRDEVAASDPDFAGRLA
                     AKTMLGRTGRQSEIRGPLLFLATKASSFVTGAAIAVDGGWTAW"
     gene            1797..>2309
                     /locus_tag="VG274_02650"
     CDS             1797..>2309
                     /locus_tag="VG274_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWE05578.1"
                     /translation="MNMAFLSSFRAGQDTVYPDALRARLHNFPLLQDIGDPALRRLLA
                     EANWFGLPGGMELKRDGENASALFLVVTGSLGVFVQDEHEKRRLVAHIPAGETVGEMS
                     LISGDNAHSAELVALRDTELLRISPQGFESLLSRHPRVMLNLMRVLVKRLQATTRNAS
                     DSARPKTFAII"
BASE COUNT          397 a          744 c          729 g          439 t
ORIGIN      
        1 gatgtcccta aatgtcgtaa agcggaaatt taccatgccg tgacacgtta tctcgcggaa
       61 gagggtgacg tttcaatgcg tggtcggtct ccggccgagc tcgtcatggg ttcggtgcaa
      121 ttgggtttgg cgtacggcgc ggccaaccgc accggaaagc cgtcgcatga gacggcgctt
      181 cggctcgtcc atcaggcggt cgccggcggc atctcgcaat tcgacaccgc gcgtgcctat
      241 ggcgacagcg aggatcgcct cggcgaagcg ctcgcggatc gcaaagatgt ccgcacgatc
      301 acgaaactca gcgccttgag cgaactagcg cccaatgcgt cgcgcgatga agtccatgcc
      361 gccgtcgatg caagcattga cgagtcgctc aacgcccttc gccgcggccg ccttgattgt
      421 ctgttgctac atcgcgccga acatatgcat gcgttcggcg gcgcggtctg gcaaaggctt
      481 atcgagcggc tggaagacgg cagcgtgttg tcgctcggcg tctccgtcca gaatccgctg
      541 gaagccctcg cggcgctcgc atgccccgac gtcgtgcatc tgcaattgcc gttcaatctg
      601 ctggactggc gctggcggaa ggcgggattc aagacctgcc tcgcgtcgcg tcacaatgtc
      661 accgttcatg cgcgcagcac gtatcttcag ggaattcttg cgagcaacga tgtgcgtgtc
      721 tggccgcgca tcgatggtgt ctatccgcag gcgctgatca acatcatcgc cggccttgcg
      781 caccagttgg gccgcgaggg cgccgccgat ctctgtctcg cctatgcgcg cggtcaggac
      841 tggatcgatg gcgtcgtggt cggtctcgaa accgaagagc aactcgacga caatctgcgg
      901 ctcttcatca agcgcccgct gagcgccgac gagtgcgcgc tggtcgaggc gcgcatcccg
      961 catcttccga tcgcgctgct caatcctgca gcgtggccga agtgagcgaa ctgtttcgtc
     1021 tcgatggttg tgtagcgttc gtgtccggcg ccgcgggcca tctcggccgc gaaatgacaa
     1081 atgcgctgtg cgaacagggc gcccacgtca tcgtcaacgg ccgcgacgac gcgaggctcg
     1141 ccgccttcga aagcgaactt ctcggcaaag gcttctctgt cgagcgagtt gtcttcgatg
     1201 tcggcgacgc tacctctgtc cgcgaattct tctctaagct gtcgcgcctc gatgtactgg
     1261 tgaacaacgc gatcagcatg acgcccaagc cgttcgcgca attggagcct gccgatttcg
     1321 agaagaccta tgccagcagc gtgaccgccg ccttcgaatc ggtgcgcgcc gcgcgtcctg
     1381 cgctggccgc ggcggcggaa gccactggcg atgcaagcgt cgtcaacatc gcatcgatgt
     1441 acgccagcgt ctcgcccgac tcgcgcatct acgcgaagcc ggagcaggcg agtccctttc
     1501 actacggacc ggccaaggcg gcgctgcttc agctgacccg gcacctcgcc gccgaactgg
     1561 gcgatcagcg gattcgcgtg aacgctttgg ttcctggacc gtttccacgg gacgaagtcg
     1621 ccgcgagcga ccccgatttc gccgggcggc tggccgccaa gacgatgctg gggcggacgg
     1681 ggcggcagag tgaaatccgg gggcctctcc tgtttcttgc aacaaaggcg tcgagttttg
     1741 tgaccggcgc cgccatcgcg gtcgatggcg gttggaccgc ctggtgagat agaacgatga
     1801 acatggcatt cctttcctcc ttccgcgccg gccaggacac cgtctatccg gacgcgttgc
     1861 gggcccggct gcacaatttc cccctgctgc aggacatcgg cgatccggca ctgcgccggc
     1921 ttctggcgga ggcgaactgg ttcggtctgc cgggcgggat ggaattgaag cgcgacggcg
     1981 agaacgcctc ggcactcttt ctggtcgtga ccggaagcct tggcgtgttc gtccaggacg
     2041 agcacgaaaa acgccgcctg gtcgcgcata tcccggcggg cgagaccgtc ggcgagatgt
     2101 cgctgatctc cggcgacaat gcgcattcgg ctgaactcgt cgcgctgcgc gataccgagc
     2161 tgctgcgcat ctcgccgcaa ggcttcgaat ccctgctcag ccgtcacccg cgcgtgatgc
     2221 tcaacctgat gcgtgttctc gtgaagcgcc ttcaggcgac gacccgcaat gccagcgaca
     2281 gcgcccggcc caaaacgttc gccatcata
//
LOCUS       DASZMC010000133         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_338380,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000133 DASZMC010000000
VERSION     DASZMC010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338380"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VG274_02655"
     CDS             complement(<1..833)
                     /locus_tag="VG274_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWE05579.1"
                     /translation="MSKLPDFEALAIFAKVAETRSFSGAAAELSLSKATVSKAVSRLE
                     ARLGARLFNRTSRRLALTDAGRQLSVRAAAILAEGEAAESEALAQSVSPRGLVRLAVP
                     MSFGLQHVAPILPEFLKQYPDVQIDLHLSDAMVDLIGEGFDAALRIATLPDSSLVARR
                     LCSVTPYLVAAPSYLAKHGAPKHPLQLAELACIGYAYLQTPNTWRFTHKSGEVASVRT
                     AGPLRVNNGDAMVPALIAGLGLGVLPDFILRKSLKDGSLKIVLPEWSLGKSGLHWVTP
                     PG"
     gene            941..1639
                     /locus_tag="VG274_02660"
     CDS             941..1639
                     /locus_tag="VG274_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWE05580.1"
                     /translation="MIDVRPFNSLGGADHGWLKAKHHFSFADYQDPKRTQWGNLRVWN
                     DDEIAPNTGFPPHPHANMEIVTYVRKGAITHKDSLGNEGRTEAGDVQVMSAGSGIRHA
                     EYNLEPETTTLFQIWILPTRTGGQPGWGAKPFPKGDRSGRFITLASGFEDDKDALPIR
                     TDARVLGATLKAGETTEYPGGGKRHLYLVPATGAVEVNGVRVNARDGAAITDVETVKV
                     VALEDSELVLVDAA"
     gene            1723..2322
                     /gene="wrbA"
                     /locus_tag="VG274_02665"
     CDS             1723..2322
                     /gene="wrbA"
                     /locus_tag="VG274_02665"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HWE05581.1"
                     /translation="MPKVLVLYYSAYGHIEKMAEAVAEGARETGATVDIKRVPELVPE
                     AVAKASYYKMDQTAPVATIDDLANYDAIVVGTGTRFGRMTSQMANFLDQAGGLWAKGA
                     LHGKVGGAFTSTATQHGGQETTLFTIITNLLHFGMVVVGLDYGHAGQMTLDEITGGSP
                     YGATTITGGNGARQPSENELQGARYQGRKIAETAKKLHG"
     gene            2346..2822
                     /locus_tag="VG274_02670"
     CDS             2346..2822
                     /locus_tag="VG274_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWE05582.1"
                     /translation="MARAQAKIGTVDYRTTITAGRHELIADEMPTLGGGDAGPVPYEL
                     LLASLGACTAITLRMYAERKKWPLETVEVSLHYERKGDVQRIERTLRFTGALEESQRA
                     RLADIAERTPVTLTLKGGLPITTDVDPPLAAAMLDAKLDEALKESFPASDPPSIVR"
BASE COUNT          590 a          875 c          890 g          504 t
ORIGIN      
        1 cccggcggcg tcacccaatg caggcctgac ttgcctagcg accattcggg tagaacgatc
       61 ttcagcgatc cgtctttcag tgatttgcgc aggatgaaat ctggcagcac acctaagcca
      121 agtcccgcga tcagcgcagg caccatcgcg tcgccattgt tcacgcgcag cggacctgca
      181 gtacgcacgc tcgcgacttc accgctcttg tgcgtgaaac gccacgtgtt cggcgtctgc
      241 agataggcgt agccgatgca ggcgagttcg gcgagttgga gcggatgttt cggcgcgcca
      301 tgtttcgcaa gataagacgg cgccgcgacg agatacggcg tcacgctgca cagcctgcgc
      361 gcgaccagcg acgaatccgg cagggtcgcg attcgtagcg cagcgtcgaa tccctcgccg
      421 atcaaatcga ccatcgcgtc actcagatga agatcgattt gcacgtctgg gtactgtttc
      481 agaaactccg gcaggatcgg cgccacatgc tggagcccga acgacatggg cacggcgagg
      541 cgcacaagcc cacgcgggct gaccgattgc gccagcgcct cgctctccgc cgcctcgccc
      601 tccgccagga tggctgccgc ccgcacagag agctgccgcc cggcgtccgt gagcgccagc
      661 cgacgggagg tgcggttgaa caggcgtgcg cccaggcggg cttccagacg gctgacagcc
      721 ttggaaaccg tggctttgga aagacttagc tctgccgccg cgcccgagaa ggagcgggtc
      781 tctgccactt tggcgaagat cgccagagcc tcgaaatcgg ggagtttcga catatcaaaa
      841 ccggaaacaa tgagtttcga ttgtttctat ttttatttgc ggccgctgtc gatatctctg
      901 gtgccagaaa acggcggcaa ctccgccaaa ggagcatcca atgattgacg tcagaccctt
      961 caattccctc ggcggcgcgg accatggctg gctgaaggcc aagcaccatt tctccttcgc
     1021 cgactatcag gaccccaagc gcacgcaatg gggcaatttg cgcgtctgga acgacgacga
     1081 gatcgcgccc aacaccggct tccccccgca tccgcatgcg aacatggaga tcgtgacata
     1141 cgtccgcaaa ggcgcaatca cgcacaagga cagcctcggc aatgaaggcc gcaccgaagc
     1201 gggcgacgtg caggtgatga gcgcgggctc gggcatccgc cacgccgaat acaatctcga
     1261 acccgagacg acgacattgt tccagatctg gatcctgccg acgcgcacgg gcggtcagcc
     1321 gggctggggt gcgaagcctt tcccgaaagg cgatcgctcg ggacgtttta tcacgctggc
     1381 gagcggcttt gaggatgaca aggacgcatt gccgatccgc accgatgcgc gcgtgcttgg
     1441 cgcgacgctg aaagccggtg agacgaccga atatcccggc ggcggaaagc gtcatctcta
     1501 tctcgtgccg gcaacgggcg cagtcgaggt caacggcgtg cgcgtgaatg cgcgcgatgg
     1561 tgccgcgatc accgatgtcg agacggtgaa ggtcgtagca ctcgaagatt ccgaactcgt
     1621 gctggtggac gcagcctaac tcaccagcct gcgggcacga tccttagccc ctcctggatc
     1681 gtgcccgccc cttttttcaa ttcccaaccc aagaggtgtg tcatgcccaa agtgctcgtt
     1741 ctctattatt cagcctatgg ccatatcgag aagatggcgg aagccgttgc agaaggtgcg
     1801 cgcgaaaccg gcgccacggt cgatatcaaa cgcgtgccgg agcttgttcc ggaagcggtc
     1861 gcgaaggcgt cctattacaa gatggatcag acggcccccg tcgcgaccat cgacgatctc
     1921 gcgaattacg acgcgatcgt tgtcggcacg ggcacgcgct tcggccgcat gacgtcgcag
     1981 atggcgaact tcctggatca ggccggcggc ctttgggcga aaggcgcgtt gcacggcaag
     2041 gtcggtggtg cattcacgtc cacggccacg cagcacggtg gacaggaaac aacgctgttc
     2101 acgatcatca ccaacctgct gcattttggc atggtcgttg tcggtctcga ctacggtcat
     2161 gcaggacaga tgactctgga tgagatcacc ggcggctcac cctatggcgc caccacgatc
     2221 accggcggca acggtgcgcg ccagcctagc gagaacgagc tgcagggcgc gcgctatcag
     2281 ggtcgcaaga ttgcagagac ggcgaaaaag cttcacggtt agaggctgac ttcggagcat
     2341 cgctcatggc gcgcgcgcag gcaaagatcg gaaccgttga ctatcgaaca acgatcacgg
     2401 ctggccgcca tgagctgatc gcggacgaga tgcctacgct cggcggcggc gacgcgggac
     2461 ctgtgcctta cgagttgctg ttggccagtc ttggcgcgtg cacggcgatc acgctgcgca
     2521 tgtatgcgga gcggaaaaaa tggcctttgg aaacggtcga ggtgtccctg cactacgaac
     2581 gcaagggcga tgtgcagcgc atcgagcgga ccttgcgttt caccggtgcg cttgaagaaa
     2641 gccaacgtgc gcggctggcc gatatcgctg agcgcacgcc cgtgacgctg acgctcaagg
     2701 gcggacttcc gatcacgaca gatgtcgatc cgccgctcgc cgcagcaatg ctggatgcga
     2761 agttggatga ggcgttgaaa gagagttttc cagcgagcga cccgccgagc atcgtgcgct
     2821 gacgttcagc ccttcaacgt tacatcgaca tctgcggaa
//
LOCUS       DASZMC010000134         4756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4451897,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000134 DASZMC010000000
VERSION     DASZMC010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4451897"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VG274_02675"
     CDS             <1..200
                     /locus_tag="VG274_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05583.1"
                     /translation="GWRSLPRNTPSPHGFDIASALLSAATFGLLIGSVDALGHGEGFP
                     LFLAEMVTTIGLGYLLVRRQL"
     gene            231..908
                     /locus_tag="VG274_02680"
     CDS             231..908
                     /locus_tag="VG274_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE05584.1"
                     /translation="MKIPIFTLSVSTSISSFAAQMLAFVSLPFLFQNELHFSAVETGL
                     LITPWPLAICVAAPLSGRLADRYSAGLLGGIGLFCFSMGLLSFAFLPEHPGYLELGWR
                     MALCGFGFGIFQSPNNRAMLSAAPRERSGGASGMLGTARLLGQTIGAALAALLFARLA
                     HQAPTVGLLISSGFALVAAGVSLARLYEPTALAQQRTSDPEADPSLHATLLHGEADSS
                     AEFEKSG"
     gene            complement(909..1484)
                     /locus_tag="VG274_02685"
     CDS             complement(909..1484)
                     /locus_tag="VG274_02685"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWE05585.1"
                     /translation="MNARFLAIAAVVVIVAGIAVLYGMRTATVHAPAGPPASLARLTP
                     LKQPEVVPDVAFEGVDGARHTLADFKGHYVLLNLWAVWCAPCVKELPALAKLKASLPG
                     GRFDVVAVDVARGTPNDAKVFLDGHGASGLATYVDPQLTLMRAFGAYGLPLSILIDDK
                     GREIARAVGPAEWDAPESIDYFKTLTTKTRP"
     gene            1497..2879
                     /gene="argH"
                     /locus_tag="VG274_02690"
     CDS             1497..2879
                     /gene="argH"
                     /locus_tag="VG274_02690"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWE05586.1"
                     /translation="MSTNKMWGGRFGSGPSDIMKEFTPSIDFDKRLWRQDIAGSRAHA
                     RMLAAQKIISDADRDAILSGLDMIWKEIEEDRFVFRRELEDIHLNIESRLAEIAGEAA
                     GRLHTARSRNDQVATDFRLWVRDACAEINAALADLQRTLLKQAEAHSETIMPGFTHMQ
                     PAQPITFAHHCLAYVEMFARDHGRFADTAKRLNDSPLGAAALAGTSFPINREATAKEL
                     GFARPMRNSLDAVSARDFALEFLSAATICMTNLSRLAGELVQWTSPAFGFVRLSDAFT
                     TGSSIMPQKRNPDAAELVRGKTGRVLGDFVALATVMKGLALAYGSDLQEDKERVFDAA
                     DTLELSLRAMSGMIADLTVNKEAMRRAAEAGYPTATDLADWLVRVLKKPFREAHHIAG
                     SIVKHAEDKGVALDQVPLPEMQAVEPGITRDIFAVLSLEASVNSRESYGGTAPVRVRE
                     QIAFWRERLT"
     gene            2876..3022
                     /locus_tag="VG274_02695"
     CDS             2876..3022
                     /locus_tag="VG274_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HWE05587.1"
                     /translation="MTMRLLLAFLVLFSLAGCGVKNDLVMPDGNKTPKGEKDPSKPPI
                     PLGT"
     gene            3019..4296
                     /gene="lysA"
                     /locus_tag="VG274_02700"
     CDS             3019..4296
                     /gene="lysA"
                     /locus_tag="VG274_02700"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWE05588.1"
                     /translation="MNHFAYRGGILHAEDVDLSALADQVGTPFYCYSTATLERHYRVF
                     AEALPNDALVAFSVKANGNLAVLKTLARLGAGADVVSGGELKKALAAGIPPERIVFSG
                     VGKTRTEMQFGLDTGIHQFNVESEPELEALNEIALASDKRAPITIRVNPDVDAKTHAK
                     ITTGTSETKFGIPWNRARDAYALAARLKGIEIVGVDVHIGSQITSLAPFAEAFARVAE
                     LVRQLRADGHTISRLDLGGGLGVPYMTNNEPPPDPDSYGKLASGIATQLGARLIVEPG
                     RLIAANAGVMVSRVLYVKQGAAKTFLILDAGMNDLLRPALYDAFHEIVSVREPTTTTP
                     RRKYDVVGPVCETTDLFARDRELPELKAGDLVAFLTAGAYGAVMSSSYNARPPAPEVL
                     VKGGEWTIVRPRLSDDALIALDRLPPWLEDQAR"
     gene            4238..>4756
                     /locus_tag="VG274_02705"
     CDS             4238..>4756
                     /locus_tag="VG274_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HWE05589.1"
                     /translation="MTRLSPSTDFRPGSKTRRAEAFVTAARAALTWERVWPALWPATG
                     IAGLFIAAALFGLPQFLPWPLHALALAGCITAMGLSVYFNLENFRFPDWYDGARRIER
                     DSTLVHRPISEGEDILSAGEGDAWAQALWNAHLSRRLADFGTLRLSIPQSDLPKRDPR
                     AFRFVVLLLIVAG"
BASE COUNT          811 a         1509 c         1513 g          923 t
ORIGIN      
        1 tcggctggcg ctcgctgcca cgcaacacgc catcgccgca tggcttcgac atcgccagtg
       61 cattgctgag cgcggcgacg tttggcctgc tgattggcag cgtcgatgcg ctcgggcacg
      121 gcgaaggctt tccgctgttc ctggccgaga tggtgacgac aattgggctc ggatatctgc
      181 tggtgcggcg gcaattgtag ctcgcggcgc cgcttctgcc ggtcgatctg ttgaagatcc
      241 cgatcttcac gctgtcggtc tcgacctcga tctcgtcttt cgcggcgcag atgctggcgt
      301 tcgtatcgtt gccgttcctg ttccagaacg aattgcattt cagcgcggtg gagacaggtc
      361 ttctcatcac gccgtggccg ctggcgatct gtgttgccgc accgctctcc ggacggctcg
      421 cggaccgtta ttcggcgggc ctgctcggcg gcatcggtct gttctgcttc tcgatggggc
      481 tgctgtcgtt cgcattcctg ccggagcatc caggttatct cgaactcggc tggcgcatgg
      541 cgctctgcgg cttcggcttc gggattttcc agtcgcccaa caaccgcgcg atgctcagtg
      601 ccgcaccccg ggagcgcagc ggcggcgcca gcggcatgct cggcaccgcg cgcctgttgg
      661 ggcaaacgat cggcgccgcg ctggcggcac tcctgttcgc gcgcctggcg catcaggccc
      721 cgacggtagg tcttctgatc tcgagcggct tcgcgctggt cgccgccggc gtaagcctgg
      781 cgcggttgta tgagccgacg gcactggcgc aacaacgcac atccgacccc gaggctgatc
      841 cgtcgttgca tgccacgctg ttgcatggcg aagcagactc atctgccgag ttcgagaagt
      901 ccggttgact acggccgcgt cttagtcgtc agcgtcttga agtaatcaat cgactccggc
      961 gcgtcccatt ccgccgggcc gacggcgcgc gcgatctcgc ggcccttgtc gtcgatcagg
     1021 atgctgagcg gcaatccata cgccccgaac gcacgcatca gggtcagctg cggatcgaca
     1081 taggtggcaa ggcccgatgc gccgtggccg tcgaggaaga ctttcgcgtc gttcggcgtg
     1141 ccgcgcgcga catcgaccgc gacgacatcg aaccgcccgc cgggcaggga ggctttcagc
     1201 ttcgccagcg ccggaagctc cttcacgcac ggcgcacacc acaccgccca aagattgagc
     1261 agcacgtaat ggcccttgaa atccgccagt gtgtggcgcg cgccatcgac gccctcgaac
     1321 gccacatcgg ggacgacttc gggctgttta agcggcgtca ggcgcgcgag cgacgctggc
     1381 ggcccggccg gcgcgtggac agtcgccgtc ctcatcccat atagaacggc tatgccagcg
     1441 acgatgacga cgacggcggc aatagcaagg aaacgagcgt tcatcgtggg cttcctatga
     1501 gcacgaacaa gatgtggggc ggccgtttcg ggtccgggcc ttccgatatc atgaaggagt
     1561 ttacgccctc catcgatttc gacaaacgcc tgtggcggca ggatatcgca ggctcccgcg
     1621 cccatgcgcg catgctggca gcgcagaaga tcatttctga cgcagaccgt gacgcgatcc
     1681 tgtccggtct cgacatgatc tggaaagaaa tcgaggagga tcgtttcgtc ttcaggcgcg
     1741 agctggaaga cattcatctt aatatagagt cgcgtctggc ggagatcgcg ggcgaagcgg
     1801 cgggccggtt gcacaccgcg cgctcgcgca acgaccaggt cgcgaccgat ttccggcttt
     1861 gggttcgcga tgcgtgcgcg gagatcaacg cggcgcttgc ggatttgcag cgcacgctgc
     1921 tgaaacaagc ggaggcgcac agcgaaacga tcatgccggg cttcacgcat atgcaaccgg
     1981 cgcagccgat cacgtttgcg catcattgcc tcgcttatgt tgagatgttc gcgcgcgatc
     2041 acggacggtt tgccgacacg gcgaagcggc tgaatgattc tcccttggga gccgcggcgc
     2101 tcgcgggcac gtcgtttcct attaatcgtg aagcgacggc gaaggaattg ggcttcgcgc
     2161 gaccaatgcg caattcgctc gatgccgtgt cggcacgcga ctttgcgctg gagtttctct
     2221 ccgccgcgac aatctgcatg accaatctct cgcggctcgc gggcgaactc gtgcaatgga
     2281 cgagcccggc gttcggcttc gtgcgacttt cggacgcgtt caccaccggc tcctcgatca
     2341 tgccgcagaa gcgcaatccg gacgccgccg aactcgtgcg cggcaagacc gggcgtgtgc
     2401 ttggcgactt cgtggcgctg gccactgtga tgaagggtct cgcgctcgct tacggaagcg
     2461 atcttcagga agacaaggaa cgcgtcttcg acgccgccga cacgttggaa ctttcgcttc
     2521 gcgccatgag cgggatgatc gccgatctga cggtgaataa agaggctatg cgccgtgcgg
     2581 cagaggcggg ttatcccacc gccaccgatc ttgccgactg gctggtgcgt gttctgaaaa
     2641 agccattccg cgaggcgcat cacattgccg gcagcatcgt caaacatgca gaagataagg
     2701 gtgtggcgct cgatcaggtg ccgcttcccg agatgcaggc cgtcgaaccc gggatcacca
     2761 gggatatctt tgcggtcttg tcactggaag cctcggttaa ttcgcgcgag agttacggcg
     2821 gcacggcgcc tgtgcgcgtg cgcgagcaga ttgcgttctg gcgggagaga ctgacatgac
     2881 gatgcgcctg ttgctcgctt tccttgtcct gttttcgctt gccggctgcg gcgtgaagaa
     2941 cgacctcgtc atgccggacg gcaacaagac gccgaaaggc gagaaagacc cgtccaagcc
     3001 gccgatcccg ctcggcacat gaatcatttt gcctatcgcg gcggcattct gcacgccgag
     3061 gatgtcgatc tttcggcact ggcggatcag gtcggaacgc cgttttactg ctattcgaca
     3121 gcgacgctcg aacgccacta tcgcgtcttt gccgaagcct tgccgaatga cgcgctcgtc
     3181 gcgttttcgg tgaaggcgaa cggcaatctc gcggtgctga aaacattggc tcggctcggc
     3241 gcgggggcgg acgttgtctc cggcggcgaa ctcaagaagg ccctcgccgc aggcattccg
     3301 ccggagcgga ttgttttctc cggtgtcggc aagactcgca cggaaatgca gttcggcctc
     3361 gacaccggta tccatcaatt caatgtcgag agcgaaccgg aactcgaggc gctgaatgaa
     3421 atagcgctcg catcggacaa gcgcgcgcca atcaccatcc gcgtcaatcc cgatgtcgac
     3481 gcaaagacac acgccaagat cacgaccgga acgtcggaga ccaagttcgg aatcccgtgg
     3541 aaccgcgcgc gcgacgccta tgcgctggcg gcgcgcctga agggaattga gatcgtgggc
     3601 gtcgatgtcc atatcggcag ccagatcacg agcctcgcgc cgttcgcgga agcatttgcc
     3661 cgcgtcgccg aactggtgcg gcagttgcgc gccgatggcc acacgatctc gcgcctcgat
     3721 ctcggtggcg gactaggcgt gccgtacatg acgaacaacg agccgccgcc cgatccggac
     3781 tcctatggca aactcgcgtc cgggattgcg acgcaactcg gcgccagact catcgtcgag
     3841 cccggccgcc tcatcgccgc caatgccggc gtgatggtca gccgtgttct ctatgtgaag
     3901 cagggcgcgg cgaagacctt ccttatcctc gacgccggca tgaacgatct gctgcggccc
     3961 gcgctctatg acgcttttca cgagatcgtg tccgttcggg aaccaacgac cacgacgccg
     4021 cgccggaaat acgatgtcgt tggtccggtc tgcgaaacca cggacctttt cgcccgcgac
     4081 cgcgaactgc cggaactcaa agcgggcgat cttgtcgcgt ttctcaccgc cggggcctat
     4141 ggcgcggtga tgtcctcgtc ctacaatgcg cgcccgcccg cgccggaggt tctggtgaag
     4201 ggtggcgagt ggaccatcgt aaggccccgg ttgagtgatg acgcgcttat cgccctcgac
     4261 agacttccgc cctggctcga agaccaggcg cgctgaagcg ttcgtcactg ccgcacgcgc
     4321 cgcgctgacg tgggagcggg tgtggccggc gctgtggcct gccaccggaa tcgcgggtct
     4381 gtttatcgcc gccgcgctgt tcggattgcc gcagttcctg ccgtggccgc tccatgcact
     4441 ggcgctcgcg ggctgcatca ccgcgatggg cctctcggtc tatttcaatc ttgagaactt
     4501 ccgctttccc gactggtatg atggcgcgcg ccggatcgag cgcgactcca cgctcgtcca
     4561 tcgtccgatc tccgagggcg aagatatcct gagcgcgggt gagggcgacg cctgggctca
     4621 ggcgctgtgg aacgcgcatc tcagccgccg cctcgccgat ttcggaacgt tgcgtctgtc
     4681 gatcccgcag tctgatctgc caaagcgcga tccgcgcgcg tttcgtttcg ttgtcctgct
     4741 gctgatcgtt gcgggc
//
LOCUS       DASZMC010000135         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_874699,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000135 DASZMC010000000
VERSION     DASZMC010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874699"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VG274_02710"
     CDS             complement(<1..1161)
                     /locus_tag="VG274_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HWE05590.1"
                     /translation="MKIHTKAVHTGDRKKLGSHIPVTTPIYTASAYSYDSMEQLDRIF
                     GQEEQGPCYARYETPSSAALEELVAALENGAGAQSCASGMAALHLAVTAALVDRRRAV
                     LAANNLYGATTGLVMNVFEPAGVEVRFADPCDLDAFRSTVEEFKPGCILIESISNPLL
                     RVSAIDKIAELARAAGAALVVDNTFGTPLVVRPLELGAHFSVHSLTKYLAGHGDVLGG
                     AIVTDEALLPTVRSLSRTLGPTLGPFECYLTMRGIKTFPLRMERQCANACRIATWLAT
                     HPKVERVYFAGDPAHPDAATVRRLFQPNLAGAIVSFELRDAARPEVFKFMDALRLIVR
                     ATSLGDVHTMMLYPAMSSHRELSPKHRERMGIRDNLVRLSAGIESPEDLIADL"
     gene            complement(1166..>1636)
                     /locus_tag="VG274_02715"
     CDS             complement(1166..>1636)
                     /locus_tag="VG274_02715"
                     /inference="COORDINATES: protein motif:HMM:NF020752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HWE05591.1"
                     /translation="LRSAHTAIERLRNFKLRLETDKYPDGSNEQLAARTQQAETAFVE
                     AMDHDLNTAEALAAAFEYIRDANSAMDAGEFRASDATAALQFLSRFDAVFDVIAPTTA
                     TNAIADSEIESLVAERNSAKKAKNFARADQIREQLLDQGIILEDTKSGVRWKRK"
BASE COUNT          314 a          444 c          554 g          324 t
ORIGIN      
        1 gagatcggcg atgaggtcct cgggcgattc gatgccggca gagagacgca ccagattgtc
       61 gcgaattccc atgcgctcgc gatgcttcgg cgaaagctcg cggtgagagc tcatagccgg
      121 atagagcatc attgtgtgca cgtcgccgag tgaggtggcg cgcacaatca gccggagcgc
      181 atccataaat ttgaatacct cgggacgcgc ggcatccctc agttcaaaac tgacgatagc
      241 gccggctaga ttgggctgga ataatctacg cacggtggcg gcgtcgggat gggcgggatc
      301 accggcgaaa tatacacgct ccactttcgg gtgcgtagcc aaccaggtcg cgatgcggca
      361 tgcgttggcg cactggcgct ccatacgcaa gggaaacgtc ttgatgccgc gcatcgttag
      421 gtaacactcg aacggcccga gcgtcgggcc gagcgtgcgc gagaggctgc ggacggtcgg
      481 gagaagagcc tcatcagtga caatagcgcc gcccagcacg tcgccatgcc cggcgagata
      541 tttcgtcaga ctatgcaccg agaagtgcgc gcccagttcc agagggcgaa caacgagcgg
      601 cgtgccgaat gtgttgtcga ccacgagcgc tgctccggcc gcgcgcgcca gttccgcgat
      661 cttgtcgatt gcggagacgc gcagcagcgg attcgagatc gattcaatca aaatacaacc
      721 gggtttgaac tcttcgacgg tcgagcggaa agcatcgaga tcgcagggat cggcaaagcg
      781 cacttccacg ccggcgggtt cgaaaacgtt catgacgagc ccggtggtgg cgccgtagag
      841 attgttggcg gcgagcacgg cgcggcggcg atccaccagc gcggcggtca cggccaggtg
      901 tagggcggcc atcccggaag cgcaggattg cgccccggcg ccgttttcga gcgcggctac
      961 aagctcttca agtgctgccg atgagggcgt ctcgtagcgc gcatagcagg ggccttgttc
     1021 ttcctggcca aagatgcgat cgagctgttc catcgagtca taggagtaag ccgatgcggt
     1081 gtaaatcggc gtcgtgacgg gaatgtgcga gcccagtttt ttgcggtcgc cggtgtggac
     1141 cgctttggta tgaattttca acgaactatt tcctcttcca gcgcacgccg gatttagtgt
     1201 cctcgagaat gatcccctga tccaggagtt gttcgcggat ttgatccgcg cgcgcgaagt
     1261 ttttagcttt tttggctgag ttgcgttcgg ccacaagcga ttcgatctcg gaatcggcga
     1321 tggcgttggt tgcggtggtg ggtgcgatga cgtcgaacac ggcgtcaaag cggcttaaga
     1381 attgaagcgc ggcggtggcg tcggaggcgc ggaactctcc ggcatccatg gcgctgttgg
     1441 cgtcgcggat gtattcgaag gcggcggcca gcgcttcagc ggtattgagg tcgtgatcca
     1501 tggcctcgac aaacgcggtc tcggcctgct gggtacgcgc ggccaattgt tcattggagc
     1561 cgtcggggta cttgtcggtc tccaggcgaa gcttgaaatt gcgcagccgc tcgattgcgg
     1621 tgtgtgcgga gcgaag
//
LOCUS       DASZMC010000136         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_874916,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000136 DASZMC010000000
VERSION     DASZMC010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874916"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VG274_02720"
     CDS             <1..437
                     /locus_tag="VG274_02720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWE05592.1"
                     /translation="IDFAVPSGTPVMAAGAGTIQQEGWFGGYGNFVVLNHGNGYATAY
                     GHLSRFAPGVHRGSHVRQGQVIAYSGATGLATGPHLHYEIRINNIQVNPASVKVARGR
                     ILSGSELRDFQISRIHVDSEIASLPLEGKIADTATDLRAGKD"
     gene            complement(453..>2891)
                     /gene="clpB"
                     /locus_tag="VG274_02725"
     CDS             complement(453..>2891)
                     /gene="clpB"
                     /locus_tag="VG274_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HWE05593.1"
                     /translation="SELGKVPRIEGSGAGQAYLAPEMARTLDSAQELAKKAGDSFVTA
                     EILLLALVLASGSDAARILRDANVRPQNLNKAIKELRQGRTADSATAENAYDALKKYA
                     RDLTELARSGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRI
                     VNGDVPESLRDKRLMALDMGSLIAGAKFRGEFEERLKAVLSEITAAAGGIVLFIDELH
                     TLVGAGKAEGAMDASNLLKPALARGELHCVGATTLDEYRKHVEKDAALARRFQPVFVS
                     EPTVEDTISILRGLKEKYEVHHGVRITDGAIVAAAQLSHRYIADRFLPDKAIDLMDEA
                     ASRLRMQIDSKPEALDELDRRIIQLKIEREALKKESDKASKDRLAALEHELADTEEKS
                     ASLTARWLEEKKSVQDVQGLKEKLDAARQELDVAQRRGNLARAGELTYGVIPELERKL
                     KIIEDKEGSELTNEAVTAEHIAGVVSRWTGIPVDKMLEGERERLLHMESSLEKRVVGQ
                     HQAVRAISNAVRRARAGLQDPNRPIGSFLFLGPTGVGKTELTKALAAFLFDDDSAMVR
                     IDMSEFMEKHSVARLIGAPPGYVGYEEGGVLTEAVRRRPYQVVLFDEVEKAHHDVFNV
                     LLQVLDDGRLTDGQGRTVDFRNTVIILTSNLGTEFLSTGESGESPQARAQVMEAVRAH
                     FRPEFLNRLDEIILFHRLSRSDMDTIVDIQIARLARLLADRKIEIVLDAKARDWLATA
                     GYDPVYGARPLKRVIQRRLQDPLAQLLLEGTIGDGAKVKVSAGKNGLIIDGETFDTGD
                     DYLDEPSVSSRAVH"
BASE COUNT          498 a          938 c          867 g          588 t
ORIGIN      
        1 gcattgattt cgcggtacct tcaggaacgc cggtgatggc ggccggtgcg ggaacgatcc
       61 agcaggaagg ctggtttggc ggctatggca attttgtcgt gctgaatcac ggcaacggct
      121 acgcgaccgc ctatggccat ctctcgcgct tcgcgccggg cgttcaccgc ggttcgcatg
      181 tgcggcaggg ccaggtgatc gcctatagcg gcgccaccgg cctcgccacg ggtccccatc
      241 tgcactacga aatccgcatc aacaacatcc aggtcaatcc tgcgagcgtg aaagttgcac
      301 gcggccgtat tctgtcgggc agcgagctgc gcgatttcca gatttcgcgt attcacgtcg
      361 acagcgagat cgcttccctt ccgctcgaag gaaagatcgc cgacaccgcg acggacctcc
      421 gcgccggcaa ggattgaagc tcgcgcgttt cgtcagtgga cggcgcgact ggagaccgac
      481 ggctcgtcaa ggtaatcatc gcctgtgtcg aaggtctcgc catcgatgat gaggccgttc
      541 ttgccggcgc tgacctttac cttcgcccca tcgccgatgg tgccttccag caagagctgc
      601 gccagcgggt cctgcagcct ccgctgaatc acccgcttga gcggacgcgc accgtagacg
      661 ggatcgtagc ccgcagtcgc gagccaatcc cgcgctttcg catcgagaac gatttcaatc
      721 ttgcgatccg ccagcagtct tgcgagccgc gcgatctgga tgtcgacgat cgtgtccatg
      781 tcggagcgcg agaggcggtg gaacaggatg atctcgtcga gccggttcag aaactccggt
      841 cggaaatgcg ccctcacggc ttccatgacc tgggcgcggg cttgcggcga ttcgccgctc
      901 tcgccagtcg agaggaactc ggtgccgaga ttggaggtga ggatgatcac cgtattgcgg
      961 aaatcgacgg tgcgtccctg cccgtcggtc aggcggccgt cgtcgagaac ctgcagcaga
     1021 acgttgaaga cgtcgtgatg cgctttctcg acctcgtcga acagcacgac ctgatacgga
     1081 cgtcggcgca ccgcttccgt caggactccg ccttcttcat aaccgacata gccgggcggc
     1141 gcgccgatca agcgcgctac ggaatgtttc tccatgaact ccgacatgtc gatgcgcacc
     1201 atcgcgctgt cgtcgtcaaa cagaaatgcc gcgagcgcct tggtgagttc ggtcttgccc
     1261 acccccgtcg gaccaagaaa gaggaacgaa ccgatcggcc gattggggtc ctgcaggccg
     1321 gcgcgggccc ggcgcaccgc attcgaaatc gcgcgcaccg cctggtgctg gccgacgacc
     1381 cgcttctcga gcgaactctc catgtgcagg agcctctcac gctccccttc gagcatcttg
     1441 tcgacgggaa tgcccgtcca gcgtgagacg acgccggcaa tgtgctccgc cgtgaccgct
     1501 tcgttggtca gctccgaacc ttctttgtcc tcaatgatct tgagcttgcg ttcgagttcg
     1561 gggatcacac cgtaagtaag ctcgccggcg cgcgcgagat tgccccggcg ctgcgccaca
     1621 tcgagttcct gccgcgccgc atccagcttt tccttgaggc cctggacatc ctgaaccgat
     1681 ttcttctcct ccagccaccg cgccgtcagc gaagccgatt tttcctcggt gtccgccagc
     1741 tcgtgctcaa gcgcggcaag gcgatccttc gatgccttgt cggattcctt tttgagcgcc
     1801 tcgcgctcga tcttcagctg aatgatgcgc cggtcgagtt cgtcaagcgc ttcgggcttc
     1861 gagtcgatct gcatccgcag ccggctcgcc gcctcgtcca tgagatcaat cgctttgtcc
     1921 ggcaggaagc ggtcggcgat gtaacggtgc gacaattgcg cggccgccac gatggcgccg
     1981 tcggtgatgc gaacgccgtg atggacctcg tacttctcct tcagtccccg caggatggag
     2041 attgtatcct cgacggtggg ctcggacacg aagaccggct gaaagcgacg ggccagtgcc
     2101 gcatcctttt ccacatgctt gcggtattcg tcgagcgtgg tcgcacccac gcaatgcagc
     2161 tcgccgcgcg cgagtgccgg cttcaaaaga ttcgaggcgt ccatcgcgcc ttcggccttg
     2221 ccggcgccca cgagcgtgtg gagctcgtcg atgaaaagca cgatcccgcc ggcagccgcc
     2281 gtgatttccg aaagcaccgc cttgagccgc tcctcgaatt caccgcgaaa cttcgctccg
     2341 gcgatcaggg agcccatatc gagcgccatc agacgcttgt cgcggaggct ttcgggtacg
     2401 tcaccgttga cgatccgcaa tgcaagtcct tcggcgatcg ccgtcttgcc cacacccggc
     2461 tcgccaatga gaacggggtt gttcttcgtg cgccgcgaca gaacctggat tgtgcggcga
     2521 atttcctcgt cgcgcccgat cacgggatcg agctttccgc ttcgcgcgag ttccgtcaga
     2581 tcgcgtgcat atttcttgag cgcgtcataa gcattttcgg ctgtcgcgga atctgccgtc
     2641 ctgccctgac gcaattcctt gatggccttg ttgagatttt gcggacggac atttgcgtcc
     2701 ctgaggatgc gcgcagcatc gcttccgctg gcgagcacga gtgccagaag cagaatttcg
     2761 gcggtgacga agctgtcccc ggccttcttt gccagttcct gggcagaatc gagcgtacgc
     2821 gccatctcgg gcgcaagata agcttgtccg gcgcccgagc cctcgatacg cggcaccttg
     2881 ccgagttccg a
//
LOCUS       DASZMC010000137         4340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1974323,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000137 DASZMC010000000
VERSION     DASZMC010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974323"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VG274_02730"
     CDS             complement(<1..971)
                     /locus_tag="VG274_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE05594.1"
                     /translation="MPNDAAANDDAEIDRISEASRAIRPLRDSRLREAAQALNANRPD
                     IAGQVLSEFLRAHPRDADALNLMGDAALRLDRKIDAEEWLARCVAEAPGFDAARFNYA
                     DALRQMNKPVAAMAQLDVLLEKAPRNPLYRDLKAVLLAALGHHADSLACRRALVEDYP
                     NSANALVTCAHALRTMGFQDECIAAYRKAIGISPTFGIAWWGLANLKMFRFAATDIER
                     MEEVLAGPGLAQPDRVRLLFALGKAHGDLARYEASFQCYAKGNAIRRLGVNYDPDVTT
                     LNARRYKELFTPEFFRDRTGSGSAARDPIFVVGLQRSGSTLVEQILA"
     gene            complement(974..1942)
                     /locus_tag="VG274_02735"
     CDS             complement(974..1942)
                     /locus_tag="VG274_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWE05595.1"
                     /translation="MPATLEAVRPISLSEIREARERIVGTILRTPLVRLELGPRGPDI
                     RLKLETLQPINAYKLRGAANAVAMLSEAERKRGVWTISAGNAGQGVAYAAAKAGVPCV
                     VVVIETAPASKIERMKALGARLVPVPYEVAWRALDERSYPGVEGTFVHPFDDHNFIAG
                     HATLGLEILEDAPDTGTIIAGIGGGGLVVGVASAVKALKPAIKVFGAEPETAAPAALS
                     FATGSPQVFKEWKSSFVDGAGGQSLFPRMWRRMQGVVDGSLVVSLEETKRAMRLMAEK
                     TRIISEGAGALPLAAALSGKAGDGPIVAIVSGGNIDLKKFCELIEA"
     gene            2463..3332
                     /locus_tag="VG274_02740"
     CDS             2463..3332
                     /locus_tag="VG274_02740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWE05596.1"
                     /translation="MKIGIFVAFVSPLATPELILGFGRRAEEAGLDSIWMGEHVVLFD
                     RMEFPYPGSKDGRIPVPEGGGILEMVATFGFLAAATKRIRLGSGICLVPQRNPVYTAK
                     EFATLDWLTGGRIDFGVGVGWCKEEVIACGYRWEDRGERCDEILELMREVWTQPVASF
                     DGKHVSLAECRMDPKPVQKPHIPIFVGGHSAAAMKRAARFGAGWYGFALGPDAARAAL
                     ERLDAALAEADRKRGADFQIAITPPYAVTQDMVRAYAALGVDRLIVQLGSQHQDKIDS
                     RLLELEKFVGAAD"
     gene            complement(3363..4040)
                     /locus_tag="VG274_02745"
     CDS             complement(3363..4040)
                     /locus_tag="VG274_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWE05597.1"
                     /translation="MKDGPSIAPTAALAGDPARANMLAALTSGMALTASELAAEAGVT
                     LQTASSHLAKLEAGGLIEASKAGRHRYFRLANDDVAAMLEAIMGVAARTGQMRTRPGP
                     RDCAMRKARVCYDHLAGEMGVQLLDSLVKTQRVARNADALRLTRRGEAFMQDFRIDIR
                     GLATARRPLCKPCLDWSMRREHLGGGLGAALLERFHELKWIDRDGSSRVLRFTRPGEA
                     GFGRMFE"
     gene            4114..>4340
                     /locus_tag="VG274_02750"
     CDS             4114..>4340
                     /locus_tag="VG274_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HWE05598.1"
                     /translation="MSIVCHIRYRIDPFRRADFEEYARNWLTIIPRCGGDPIGYFMPH
                     EGTNDIAHALIEFESLAAYEIYRAKLRTDPDA"
BASE COUNT          757 a         1386 c         1429 g          768 t
ORIGIN      
        1 cttgcgagaa tctgttcgac cagcgtcgag ccggaacgct gcaggccgac cacgaaaatc
       61 ggatcgcggg cggctgagcc cgagccggtc cggtcgcgga agaattcggg cgtgaacagt
      121 tctttgtagc gtcgcgcatt cagcgtcgtc acgtccggat cataattgac gccgagacgc
      181 cggatggcat tgcccttcgc atagcactgg aaggacgcct catagcgcgc gagatcgcca
      241 tgggccttgc cgagagcaaa tagcagacgc acgcgatccg gctgggcgag gccgggccct
      301 gcaagaacct cttccatccg ttcgatatcg gtggccgcga agcggaacat tttgagattt
      361 gcgagtcccc accacgcgat gccgaaagtt ggcgaaatcc cgatcgcctt gcgataggcg
      421 gcaatgcatt cgtcctggaa ccccatggtt ctcagcgcgt gggcgcaggt cacgagggca
      481 tttgcggagt tcggatagtc ttccacgagg gcgcggcggc aggcgaggga gtccgcgtga
      541 tgtcccaatg cggccagaag aaccgccttg agatcgcgat agagcggatt gcgcggcgcc
      601 ttttcgagca gcacatcaag ctgcgccatc gccgcgacgg gcttgttcat ctgccggagc
      661 gcgtctgcgt aattgaaccg cgccgcatcg aagccgggcg cctccgcgac gcagcgcgca
      721 agccattcct cggcgtcgat cttgcggtcg aggcgcaagg cggcatcgcc catcaggttg
      781 agcgcatcgg catcgcgcgg atgcgctctc agaaattccg acaggacttg tccggcaatg
      841 tccggccggt tcgcgttcag cgcctgcgcc gcctcccgca gccgcgaatc acgcagcggc
      901 cggatcgcgc gcgacgcctc cgagatacga tcgatctcgg cgtcgtcgtt tgcagctgcg
      961 tcgttcggca ctctcacgcc tcgatcagct cgcagaattt cttgagatcg atattgccgc
     1021 cggagacgat ggcgacgatc gggccatcgc cggctttccc ggagagcgcg gcggcgagcg
     1081 gcagtgcgcc tgcgccttcc gagatgatgc gagtcttttc cgccatcagc cgcatcgcgc
     1141 gtttcgtttc ctcgaggctg acgacgagcg aaccgtcgac cacgccctgc atccttcgcc
     1201 acatgcgggg gaacaggctc tggccgccgg cgccgtcgac gaaagaggat ttccactcct
     1261 tgaacacctg cggcgatccc gtggcgaacg agagcgccgc cggcgcggcc gtttcgggtt
     1321 cggcgccgaa gaccttgatc gcgggcttca gcgccttgac ggcgctggcg acgcccacga
     1381 ccagcccgcc gccgccgatg ccggcgatga ttgtccctgt gtccggcgcg tcttcgagga
     1441 tctcgagacc cagcgttgca tggccggcga tgaaattgtg gtcgtcgaaa ggatgcacga
     1501 aggtgccttc gactccggga taggaacgct cgtcgagcgc ccgccacgcc acctcgtagg
     1561 gcaccggcac gagacgcgcc ccgagcgcct tcatgcgttc gattttcgac gcgggcgcgg
     1621 tctcgatcac caccacaaca cagggcactc ccgccttcgc cgccgcatag gcgacgcctt
     1681 gcccggcgtt ccccgcgctg atcgtccaca cgccgcgctt gcgttccgct tcggaaagca
     1741 tggcgaccgc attcgccgcg cccctgagct tgtaggcgtt gatcggctgg agagtttcga
     1801 gcttcaggcg aatgtcgggg ccgcgcggtc ccagttccag gcggacgaga ggcgtgcgca
     1861 ggatcgtgcc cacgatgcgt tcgcgcgctt cgcggatttc cgaaagtgaa atcggacgca
     1921 cggcttccag cgtggcgggc atatctccaa acacctgtct ggcgatgggt ctgcgctgcg
     1981 ttctgcgcca gatgcccgcc ccgcgacaag atcagggtgc ggatcgtgcc ggagcaagcg
     2041 ttttcctcgt cctgagcttg cctgcctccg ccgaccccct gcgccgcgcg cgcgagccgc
     2101 ggcgcgagcg aagcttcagc ttcgcaccgt caggtcgaag gacgaccttg tcgaaggacg
     2161 acatggggtg acaggccgac agcccagtgt accccaggga actcgacgac gactatgccg
     2221 ccagcctgtc gctctcaatt tagcacaacc atgagggggg caatgtcggg cagcgcctga
     2281 catgcgaacc cggcttgcgc gcccgttccg gtttgagcga aaggtctcgt cagggctgct
     2341 tcggcggcgg cgcagcggcg cgagcctgcg cagctttctg ttcgcgcgcc ttgaccatga
     2401 ccgcgagaat ttgcgaggcg attgggtcag tttctccggg acgcaaatag ggacgagggc
     2461 aaatgaaaat cggcatcttc gtggccttcg tgtcgccgct ggcgacgccg gagctgattc
     2521 tgggtttcgg ccgccgcgcg gaagaggcgg ggctcgactc gatatggatg ggcgaacatg
     2581 tcgtgctgtt cgacaggatg gagtttccct atcccggatc gaaggacggg cggataccag
     2641 ttccggaagg cggcggaatt ctcgaaatgg tcgccacatt cggctttctc gccgccgcga
     2701 caaaacggat tcggctggga agcggcatct gtctggtgcc gcagcgaaac ccggtctaca
     2761 cggcgaagga attcgcgaca ctcgactggc tgacgggcgg ccgcatcgat ttcggcgtcg
     2821 gcgtcggatg gtgcaaggaa gaagtgatcg cttgcggata tcgctgggaa gaccgcggcg
     2881 agcgctgcga cgagatcctc gaactcatgc gggaggtctg gactcagcct gtggcatcgt
     2941 ttgacggcaa acatgtttcg ctcgcggaat gccgcatgga tccgaaaccg gtccagaaac
     3001 cgcatatccc gatattcgta ggcgggcaca gcgcggccgc gatgaaaagg gcggcgcggt
     3061 tcggcgcggg atggtacggc tttgcgctcg gtcccgatgc cgcgcgcgca gcactggaac
     3121 gtctcgatgc cgcgcttgcc gaagcggatc gaaagcgggg cgcggatttc cagatcgcga
     3181 tcacgccgcc ttacgcggtg acgcaggata tggtccgcgc ttatgcggcg ctgggcgtcg
     3241 atcgcctgat cgtgcaactg ggcagccagc accaggacaa gatcgacagc cgtctgctcg
     3301 aactggaaaa attcgttggc gcggcggact gaggcggcgc gccaactctt ttgaacgaaa
     3361 cactattcga acatccggcc gaatccggcc tcgcccgggc gcgtgaagcg cagaacgcgg
     3421 ctcgacccgt cgcggtcgat ccatttcagt tcgtggaagc gctccagcag tgccgcgccg
     3481 agcccgccgc cgagatgttc gcggcgcatg ctccagtcga ggcacggctt gcagagcggc
     3541 cggcgcgccg tggcaagacc gcggatgtcg atgcggaaat cctgcatgaa agcttcgccg
     3601 cgccgcgtca gcctgagcgc gtccgcgttc cgcgcgacgc gttgcgtctt cacgagactg
     3661 tcgagcaact gcacgcccat ctcgccggcc aggtgatcgt aacagacccg cgccttgcgc
     3721 atcgcgcaat cgcgcggacc gggccgcgtg cgcatctggc ccgtgcgtgc ggcaacgccc
     3781 atgatggctt ccagcatcgc cgcgacatcg tcgttcgcca gccgaaaata ccggtggcgg
     3841 cccgctttcg acgcctcgat aaggccgcct gcttcgagtt tggcgaggtg tgagcttgcc
     3901 gtctgcaacg tcacgcccgc ctccgcggcg agttcgctcg cggtgagcgc catcccgctt
     3961 gtgagcgccg ccagcatgtt ggcgcgcgcg ggatcgccgg ccagcgcagc ggtgggcgcg
     4021 atgcttgggc cgtctttcat gcttcgatgg taatcgaagc atggacgcaa aggcaacggc
     4081 tattttccct gcgggcgtca gggagaagca aagatgagta tcgtctgcca catccgctac
     4141 cggatcgatc cgttcaggcg cgcggatttc gaggagtacg ccaggaattg gctcacgatc
     4201 atcccgcgtt gcggcggaga tccgatcggc tatttcatgc cgcacgaggg cacgaatgac
     4261 atcgctcatg cgctgatcga attcgagagc ctcgcggcct acgaaatcta tcgggcgaag
     4321 ttgcgcaccg atccggacgc
//
LOCUS       DASZMC010000138         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3625010,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000138 DASZMC010000000
VERSION     DASZMC010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625010"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VG274_02755"
     CDS             complement(<1..360)
                     /locus_tag="VG274_02755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWE05599.1"
                     /translation="MTVSPNAQQVEYWNGLAGERWARLQEKIDLHVEEITDAALEFAA
                     PREGERILDLGCGCGTTTFLLAFRAGRDGTAAGIDISVPMLAVARARAMAQNANVVFI
                     EGDASTYDFQPVFDLAFS"
     gene            complement(357..1586)
                     /locus_tag="VG274_02760"
     CDS             complement(357..1586)
                     /locus_tag="VG274_02760"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWE05600.1"
                     /translation="MRKTVKKIVLAYSGGLDTSVILKWLQAAYGAELVTFTADLGQGD
                     ELAPARAKAELLGVRPENIFVEDLREEFVSAYVFPMFRANALYEGAYLLGTSIARPLI
                     AKRQIEIAERTGADAVAHGATGKGNDQVRFELAYYALNPDIKVIAPWREWDLTSRSRL
                     LEFAEVHQIPIAKDKRGEAPFSVDANLLHSSSEGKALEDPAAEPDALVYQRTLSPEQA
                     PDKPTRVEIGFRRGDAILLDGQPLSPATLLARLNELGGANGIGRIDLVENRFVGMKSR
                     GIYETPGGTILMAAHRAMESITLDRGAAHLKDELMPRYAELVYDGFWFSPEREMLQAL
                     IDTSQEFVTGTVRLKLYKGNVIVIGRSSPMSLYAKDLVTFEDDRGAYDQKDAQGFIRL
                     NALRLRTLARRRKRGMA"
     gene            1787..>3005
                     /locus_tag="VG274_02765"
     CDS             1787..>3005
                     /locus_tag="VG274_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE05601.1"
                     /translation="MTAYRHSAQSQTSVDAAGADVFAAVAAAMRATRPLRNPKLKAAA
                     DLLRQKQPEASEQLLSAYLAKHPNDPGALHLMAQALFALGRKEKADDLFARTVALAPD
                     YAAARYEYANSLFQLNKPSLALAELDWLLAADPHNPLSIDLKAIVLSAMGRHAESLVC
                     HRILAEDHPHSADIQIKYAWSLRSVGRGDECVAALRKAIALRTSCGEAYWSLAGLKNF
                     RFADEEVLAMQEQLARAEVAGEDRMYLHFALGKAYGDGKLHAKAFENYARANAIKRIS
                     IDYDPGWLTRHVAKCKTLFTPEFLRHRERTGCDARDPIFIIGMQRAGSTLVEQILASH
                     PSIEGTAELPDISLLAEHIGETIARQYGSDYPGVLDRLDASAFREFGERYLDTTRFRR
                     NHGRPFFTDKMPYN"
BASE COUNT          590 a          941 c          922 g          552 t
ORIGIN      
        1 ggaaaaggcg agatcgaaaa ccggctggaa atcgtaggtc gacgcgtcgc cttcgatgaa
       61 tacgacattc gcgttttgcg ccatggcgcg ggctcgcgcg accgcgagca tcggaacgga
      121 aatatcaatg cccgcggcag tgccgtcgcg gccggccctg aaagcgagca gaaacgtggt
      181 ggtgccgcag ccgcagccga gatcgagaat gcgttcccct tcccgcggcg cagcaaattc
      241 cagcgcggca tcggtaatct cttcgacatg gagatcgatt ttttcctgaa ggcgcgccca
      301 gcgttcgccc gcgagaccgt tccagtattc gacctgttgc gcgtttggcg agacggtcac
      361 gccatgcctc ttttcctccg gcgcgcaagt gtgcggagcc ggagcgcatt cagcctgatg
      421 aacccttggg catccttctg gtcataggct cccctgtcgt cctcgaaggt gacgagatcc
      481 ttcgcgtaaa gtgacatggg cgacgagcgg ccaatgacga tcacattccc cttgtacagc
      541 ttgagcctga ccgtaccggt cacgaactcc tggctcgtgt cgatgagcgc ctgcagcatt
      601 tcgcgctccg gggagaacca gaagccgtca tagacgagct ccgcataacg cggcatcagc
      661 tcgtccttga gatgtgcggc gccccggtcg agcgtgatcg attccatggc gcgatgcgcc
      721 gccatcaaaa tggtcccgcc aggtgtttca tagatgccgc gcgatttcat gccgacgaag
      781 cggttttcca ccagatcgat gcggccgatc ccattcgcgc ctccgagctc gttcagccgc
      841 gccagcaatg tggccggcga gagcggttgt ccgtcgagca gaatggcatc gccacgtcgg
      901 aaaccgatct cgacccgagt tggcttgtcc ggcgcctgct ccggactgag cgtgcgctga
      961 tagaccagcg cgtcgggttc cgcggcgggg tcctccaacg cttttccttc ggaggacgag
     1021 tggagcagat tggcatccac cgagaacggc gcttcgccgc gcttgtcctt cgcgatcgga
     1081 atctggtgca cctcggcgaa ttcgaggagc cgggagcggc tcgtcagatc ccattcccgc
     1141 cacggcgcga tgaccttgat gtccgggttc agggcgtaat aggcgagttc aaaacgcacc
     1201 tggtcgtttc ccttacccgt cgcgccatgc gccaccgcat ccgcgccggt tctttcggca
     1261 atctcgatct gtcttttcgc gatgagcggg cgcgcgatag aggtgccaag cagataagcg
     1321 ccctcgtaga gcgcattggc gcggaacatc gggaacacgt aggcgctaac gaattcctcg
     1381 cgcagatcct cgacgaatat attctcgggc ctgacgccca gcagctccgc cttggccctc
     1441 gcaggcgcaa gctcgtcgcc ctgcccgaga tctgcggtga atgtaaccag ctcggcgcca
     1501 taggccgcct gcagccactt cagaatgacg gaggtgtcga ggccgcccga ataggcgagc
     1561 acgatttttt tcaccgtctt gcgcaagaga attcacccgg accgttcggt ccgactatac
     1621 cgggtggaag aactcgctca agcatacacg cacatgcatg cgagacggcg accgcacact
     1681 tgcacgcagc cacggcgaca gcgtaatcgg agggcaacgc cgtggcagag cggatttctc
     1741 cgaaacagcg gttttgcccg agccggccag aaaacctgga agttcgatga ctgcgtatcg
     1801 gcacagcgcc cagagccaga catccgtcga tgcggcaggg gccgatgtgt tcgcagcggt
     1861 cgcagcggcg atgagggcga cacggccact gcgcaatccg aaactgaaag cagcggcgga
     1921 cttgctcagg caaaagcagc cggaagcatc ggagcagctg ctctcggcgt atcttgccaa
     1981 gcatccgaac gatcccggcg ccctccatct gatggcgcaa gctcttttcg cgctcggacg
     2041 gaaggagaaa gccgatgacc tgttcgcgcg cacggttgcg ctcgcgcccg attacgccgc
     2101 ggcacggtac gaatacgcga actcgctgtt ccagctgaac aagccctcgc ttgcgctggc
     2161 agagctggac tggctgctgg ctgccgatcc gcacaatccc ctcagtatcg atctcaaggc
     2221 catcgtgctg tccgcgatgg ggcggcacgc ggaatcgctc gtctgccacc ggatcctggc
     2281 ggaggatcac ccccactcgg cagatatcca gatcaaatat gcctggtcgc tgcggagcgt
     2341 aggccggggc gacgaatgcg tcgcggcgct gcgcaaggcg attgctctgc gcacgtcgtg
     2401 cggcgaagcc tattggagcc tggcaggcct caagaacttc cggtttgccg atgaggaagt
     2461 tctggccatg caggagcagc ttgcgcgcgc cgaggtcgcc ggcgaggacc gcatgtatct
     2521 gcacttcgca ttgggcaagg cgtatggcga cgggaagctc catgcgaaag cattcgaaaa
     2581 ttatgcgcgc gccaatgcca tcaagcggat ctccatcgac tacgatcccg gctggctgac
     2641 gcggcacgtt gcaaaatgca agacgctgtt cacgccggag ttcctgcgac accgggaaag
     2701 aacgggctgc gacgcgcgcg atccgatctt catcattggc atgcagcggg caggctcgac
     2761 gcttgtggag caaatcctcg caagccatcc gtcgattgaa ggaacggcgg aactgccgga
     2821 tatttcgctg ctcgccgaac acattggcga gacgatcgcg cggcaatatg gctcggacta
     2881 tcccggcgta ctggatcggc tcgatgcgtc cgcgttccgg gaattcggcg aacgctatct
     2941 cgacacaacg cgattccggc gcaaccatgg acgcccgttc ttcaccgaca agatgcctta
     3001 caatt
//
LOCUS       DASZMC010000139         3921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3365707,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000139 DASZMC010000000
VERSION     DASZMC010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3365707"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2775
                     /gene="fdhF"
                     /locus_tag="VG274_02770"
     CDS             <1..2775
                     /gene="fdhF"
                     /locus_tag="VG274_02770"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HWE05602.1"
                     /translation="DGKRTSVPLGTSILRAAAECGTDIPKLCSTDSLEAFGSCRLCLV
                     EIEGRAGTPASCTTPVAEGMIVRTQTDRLARLRRGVMELYISDHPLDCLTCAANGDCE
                     LQDMAGAVGLREVRYGYAGENHLDDAKDQSNPYFTYDPAKCIVCSRCVRACDEVQGTF
                     ALTIAGRGFESRVAASAGEAFLDSECVSCGACVQACPTATLIEKSVIEVGQPEHSVVT
                     TCAYCGVGCSFKAEMRGDTVVRMVPYKDGKANHGHSCVKGRFAWGYATHRERILKPMI
                     RERIEDPWREVAWDDAIGRVASEFKRIQARYGPRAIGGITSSRCTDEETFLVQKLVRA
                     GFGNNNVDTCARVCHSPTGYGLKTTFGTSAGTQDFDSVEQADVIVVMGANPTDGHPVF
                     ASRMKQRLRRGAKLIVIDPRRIDLVHTPHVDAAYHLPLRPGTNVAMLTALAHVIVTEG
                     LINEEFVKARCDLAAFGEWAAFVREPRNTPEAVAEITGVPAEAIRGAARLYATGGNAA
                     FYYGLGITEHSQGSTAVIAIANLAMATGNIGRPGVGVNPLRGQNNVQGSCDMGSFPHE
                     LSGYRHISDAATRSLFEADWGTHLDNEPGLRISNMLDAAVGGRFKGLYIQGEDIAQSD
                     PDTTHVIDGLKAMECVVVQDLFLCETAHYAHVFLPGSTFLEKDGTFINAERRIQRVRK
                     VMEPRNGLADWEITLAIARAMGYPMHYDHPSEIMDEIARLTPTFAGVSFDRLDALGSI
                     QWPCNEKAPDGTPVMHMGGFVRGLGNFVITEYVPTDERTGPRFPLLLTTGRILSQYNV
                     GAQTRRTKNIVWHDEDKLEIHPHDADQRGIRTGDWVKLQSRSGATSLRALVTDRVAPG
                     VVYTTFHHPDTQANVVTTDYADWATSCPEYKVTAVQVSPSNGPTDWQRAYEAESAHNA
                     RIAEPLAAAE"
     gene            2972..3799
                     /gene="fdhD"
                     /locus_tag="VG274_02775"
     CDS             2972..3799
                     /gene="fdhD"
                     /locus_tag="VG274_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWE05603.1"
                     /translation="MARRPPYANVSRRLVRHDAASHGVRAVPEEIAVAFTYGSATFAV
                     MMATPEDVEDHAVGFSLTEGIVERSSEIEGLEIVAHEHGVEARITINEKRDAQLLSRR
                     RMLAGPGGCGLCGIESLEAAVAPARPVKGDHRVDAQTIFAALRALRSGQRLNETTHAV
                     HGAGFWSVEQNRLVALREDVGRHNALDKLAGALARTGVAAETGFIVLSSRLSIELVQK
                     AAMIGCPMLVAVSAPTNYALRAAETAGMTLVAVARDDSFEIFTHPERIIIAALDETR"
BASE COUNT          698 a         1324 c         1254 g          645 t
ORIGIN      
        1 gacggcaagc gcacgagcgt gcccctgggc acctcgatcc tgcgcgccgc ggccgaatgc
       61 ggcaccgaca tccccaaact ctgctcgacc gactcgctgg aagcctttgg ctcctgccgg
      121 ctgtgcctcg tcgagatcga ggggcgggcg ggcacgcccg cctcctgcac cacgccggtc
      181 gccgaaggga tgatcgtccg tacacagacg gaccgcctcg cccgcctccg ccgcggcgtg
      241 atggagctct acatctccga tcatccgctc gactgcctca cctgcgcggc gaacggggat
      301 tgcgaactgc aggacatggc gggcgcagtc ggactgcgcg aggtgcgcta cggctatgcg
      361 ggcgagaacc atctcgacga cgcgaaggac cagtcgaacc cctatttcac ctacgatccg
      421 gcgaaatgca tcgtctgctc gcgctgcgtg cgcgcctgcg acgaagtgca gggcacgttc
      481 gcgctcacca ttgcgggccg cggcttcgaa agccgcgtcg cggcaagcgc gggagaggca
      541 ttcctcgatt cggaatgcgt ctcctgcggc gcctgcgtgc aggcctgccc caccgccacg
      601 ctgatcgaga aatcggtgat cgaggtcggc cagcccgaac attcggtcgt cacgacctgc
      661 gcctattgcg gggtcggctg ctcgttcaag gcggagatgc gcggcgacac ggtcgtgcgc
      721 atggtgccct acaaggacgg caaggccaat cacggtcatt cctgcgtcaa gggccgcttc
      781 gcctggggct acgccaccca tcgcgagcgc atcctcaaac cgatgatccg cgaacgcatc
      841 gaggatccgt ggcgcgaagt cgcgtgggac gacgcgatcg gtcgcgtggc aagcgagttc
      901 aagcgcatcc aggcgcgata cggtccgcgc gcgatcggcg gcatcacctc gtcgcgctgc
      961 accgacgaag agacgtttct cgtgcagaag ctggtgcggg cgggcttcgg caacaacaat
     1021 gtcgacacct gcgcgcgggt ctgccattcg cccaccggct acggcctcaa gaccaccttc
     1081 ggcacctccg cgggaacgca ggatttcgat tctgtcgagc aggccgacgt catcgtggtg
     1141 atgggcgcca atcccaccga cggccatccg gttttcgcct cgcgcatgaa gcagcggctg
     1201 cgccggggcg ccaagctcat cgtcatcgac ccccgccgga tcgatctcgt gcataccccg
     1261 catgtcgacg ccgcgtatca cctgccgctc cggccaggca ccaacgtggc aatgctgact
     1321 gcgctcgcgc acgtcatcgt caccgaaggc ctgataaacg aagagttcgt gaaggcgcgc
     1381 tgcgatcttg ccgcgttcgg ggaatgggcc gccttcgtgc gcgagccgcg caacactccc
     1441 gaagcggtgg cggaaatcac cggtgtgccc gcggaggcga tccgcggcgc ggcgcggctc
     1501 tacgccaccg gcggcaacgc cgctttctat tacggcctgg gcatcaccga gcacagccag
     1561 ggctcgacgg cggtcatcgc gatcgccaat ctcgccatgg cgacgggcaa tatcggccgg
     1621 ccgggcgtcg gcgtgaatcc cttgcgcgga caaaacaatg tgcagggctc ctgcgacatg
     1681 ggctccttcc cgcacgaact ttcgggctac cgccatattt ccgatgcggc gacccgcagc
     1741 ctgttcgagg cggattgggg cacccatctc gacaacgagc ccggcctgcg catctccaac
     1801 atgctcgatg cggcggtcgg cgggcggttc aaggggctgt acatccaggg cgaggacatt
     1861 gcgcaatccg atcccgacac cacccatgtg atcgacgggt tgaaggcgat ggaatgcgtg
     1921 gtggtgcagg atctcttcct gtgcgaaacg gcgcattacg cccatgtctt cctgccgggc
     1981 tcgaccttcc tcgaaaagga cggcaccttc atcaatgccg agcgccgcat ccagcgcgtg
     2041 cgcaaggtga tggagccgcg caacggcctc gccgactggg agatcacgct cgccatcgcc
     2101 agggcaatgg gctacccgat gcactacgat catccgtccg aaatcatgga cgagattgcg
     2161 cggctgacgc cgaccttcgc gggcgtgtcc ttcgacaggc tcgatgcgct cggctccatc
     2221 cagtggccct gcaacgagaa ggcgccggac ggcacgcccg tcatgcatat gggcggcttc
     2281 gtgcgcgggc tcggtaattt cgtcatcacc gaatatgtgc cgaccgacga gcgcaccggg
     2341 ccgcgcttcc cgctgctgct gaccaccggg cgcatcctca gccagtacaa tgttggtgcg
     2401 cagacgcggc gcacgaagaa cattgtctgg cacgacgagg acaagcttga gatccatccc
     2461 catgatgccg atcagcgcgg cattcgaacg ggcgactggg tgaaattgca gagccggtcc
     2521 ggcgccactt cgttgcgcgc gctcgtcacc gaccgggtgg cgcccggcgt cgtctacacg
     2581 acgttccatc atcccgacac gcaggcgaat gtggtgacga ccgactatgc cgactgggcg
     2641 acgagctgcc ccgaatacaa ggtcaccgcg gtgcaggtct cgccctcgaa cggcccgacc
     2701 gactggcagc gcgcctatga agccgaaagc gcccacaacg cccgcatcgc cgagccgctt
     2761 gccgcggcgg aatgatggcg gcgcgaattt tggcggctgt cgaacccttc gaacaagctt
     2821 ttacctcccc cttgtgggga ggtcgaaaaa cgcccttcgc gttttccggg tgggggtgga
     2881 ggcggcgccg cacacacccc cacccgaaat ttgcttcgca aatttcgacc tccccacaag
     2941 ggggaggttg aatcaacggt tcaccgctgc cgtggctcga agacctccct acgcgaacgt
     3001 atcgcgccgg ctcgtcaggc atgacgccgc atcccacggc gtgcgcgcgg ttccggaaga
     3061 gatcgcggtc gccttcacct atggcagcgc gactttcgcg gtgatgatgg cgacgccgga
     3121 ggatgtcgag gaccacgccg tcggtttctc gctgaccgaa ggaatcgtcg agcggtcttc
     3181 ggagatcgaa ggcctggaaa tcgtggcgca cgaacatggc gtcgaagcgc gaatcacaat
     3241 caatgagaag cgtgacgcgc aattgttgtc gcgccggcgg atgctcgccg gccccggcgg
     3301 ttgcggattg tgcggcatag agagtctcga ggccgccgtg gcgcccgcgc gcccggtcaa
     3361 gggagatcac cgcgtcgacg cgcaaacgat cttcgcggca ctgcgcgcgc tccgctccgg
     3421 tcagcgcctc aatgagacaa cccatgccgt gcatggcgcc ggcttctggt ccgtggagca
     3481 gaaccgcctt gttgccctgc gcgaagatgt cggccggcac aacgcgctcg acaagctcgc
     3541 cggcgccctg gcgcgcacag gtgtggcggc cgaaacggga ttcatcgtgc tctccagccg
     3601 gctgtcgatc gaactggtgc agaaagccgc gatgatcggc tgtcccatgc tggtggctgt
     3661 gtccgcgccc accaattacg ccttgcgcgc cgccgagacg gcgggcatga cgctggtcgc
     3721 ggtggcgcgc gacgattcct tcgaaatctt cacccacccc gaacgaataa tcattgcagc
     3781 cctcgatgaa acccgatgaa aaactcgcct acatggcgaa ccaggtggcg aaattcttcg
     3841 ccgcgcaggg cgaggagcgc gccgcgcgcg gcgtcgccga tcatctgcag aaattctggg
     3901 atcccgtcat gcgccgcgtc t
//
LOCUS       DASZMC010000140         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_56227,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000140 DASZMC010000000
VERSION     DASZMC010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56227"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /gene="rpsN"
                     /locus_tag="VG274_02780"
     CDS             <1..232
                     /gene="rpsN"
                     /locus_tag="VG274_02780"
                     /inference="COORDINATES: protein motif:HMM:NF006477.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HWE05604.1"
                     /translation="ALKARTQDMSVPPEERFAAHLKLAKLPRNSSKTRIHHRCELSGR
                     SKGYYRKLKLSRIALRDLSNFGQVPGMTKASW"
     gene            247..645
                     /gene="rpsH"
                     /locus_tag="VG274_02785"
     CDS             247..645
                     /gene="rpsH"
                     /locus_tag="VG274_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWE05605.1"
                     /translation="MAITDPIGDLLTRIRNGQLRGKAKVQSPNSRQRVRVLEVLQAEG
                     FIRGYAEVEFKGGRRELEIELKYHEGRPVIRELMRISTPGRRVYASVRDLRPHRSGLG
                     LSIVSTPQGVMTDTSAREKNVGGEVICRVF"
     gene            670..1203
                     /gene="rplF"
                     /locus_tag="VG274_02790"
     CDS             670..1203
                     /gene="rplF"
                     /locus_tag="VG274_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWE05606.1"
                     /translation="MSRIGKRPVPLPQGVSATVEGQSVRVKGPKGELSVILAHDVEAS
                     VAGEGVVVAPRSGAERGREMWGLSRTLVNNLVTGVTQGFTQKLEISGVGYRAAVQGKN
                     LQLQLGFSHDVAYPIPSGINIVCEKPTMITVSGIDKQLVGQVAAEIRAWRPPEPYKGK
                     GVKYAGDRIRRKEGKKK"
     gene            1206..1562
                     /gene="rplR"
                     /locus_tag="VG274_02795"
     CDS             1206..1562
                     /gene="rplR"
                     /locus_tag="VG274_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWE05607.1"
                     /translation="MASGKSLFDRRKRRTRFRVMRAAAGRPRLSVFRSGRHIYAQLID
                     DRAGQTIVTASTNEKEGKAPKTWNIEAATGVGKMIAERALAKGVKQVVFDRGGYIYHG
                     RVKALAEAARAGGLEF"
     gene            1578..2138
                     /gene="rpsE"
                     /locus_tag="VG274_02800"
     CDS             1578..2138
                     /gene="rpsE"
                     /locus_tag="VG274_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWE05608.1"
                     /translation="MAREQRQREERDSEFVDRLVHINRVAKVVKGGRRFGFAALVVVG
                     DQRGRVGFGHGKAREVPEAIRKATEAAKRAMIRVPLKDGRTLHHETRGHHGAGKVLVR
                     PAPAGTGIIAGGPMRAVFESLGVGDVVAKSLGTSNPYNMVRATFDALKLQQSPRMVAQ
                     RRGRSVSEIIARREGAPKAEQAAAGS"
     gene            2141..2344
                     /gene="rpmD"
                     /locus_tag="VG274_02805"
     CDS             2141..2344
                     /gene="rpmD"
                     /locus_tag="VG274_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009817524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWE05609.1"
                     /translation="MAEKSKSTLKVRQIRSANRRPETQAATLRGLGLGKIRRTRTLED
                     TPAVRGMIRAVQNLVEIVEESGK"
     gene            2341..2889
                     /gene="rplO"
                     /locus_tag="VG274_02810"
     CDS             2341..2889
                     /gene="rplO"
                     /locus_tag="VG274_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWE05610.1"
                     /translation="MKLNQLANNEGAHRRKTKVGRGSSSGLGKTCGRGVKGAKARTGT
                     KVYGFEGGQMPLYMRLPKRGFNNIFANDFSEVGLARIQKAIDDKKLEASRKITEGALR
                     KAGLARSSRNGVRLLGGALTSTVEIEVAGASAAAVERIRKAGGSVTLTFRKTVRTDKK
                     GQPGKKAQRRAKAAEKRAARAS"
     gene            2941..>3260
                     /locus_tag="VG274_02815"
     CDS             2941..>3260
                     /locus_tag="VG274_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05611.1"
                     /translation="MASAAEQLAANFNFSNFGKAKELRQRIFFTLALLVVARLGTFIP
                     MPGIDPSELARQLSQQQGGVLDIFNMLSGGAVQRMAIFALGIMPYISASIIIQLMTTV
                     FPEL"
BASE COUNT          682 a          936 c         1109 g          533 t
ORIGIN      
        1 ggccctcaaa gcgcgcacgc aggacatgtc tgtgccgccc gaggagcgct tcgcggcgca
       61 tctcaaactt gccaagttgc cgcgcaactc gtcaaagacg cgcatccatc accgctgcga
      121 attgtccggg cggtccaaag gctactaccg caagctcaag ctctcgcgca tagcgcttcg
      181 cgatctttcg aatttcgggc aggtcccggg catgaccaag gcgagctggt aggaaaggga
      241 caggacatgg cgatcacgga tcccattggc gatctcctca ctcgtatcag aaatggtcag
      301 cttcgcggca aggcgaaggt gcagtctccg aactcgcgcc agcgcgtacg cgtgctggaa
      361 gtgttgcagg cggaagggtt catccgcggg tatgcggaag tcgaattcaa gggcggccga
      421 cgcgagctcg agatcgagct caaataccac gaaggccggc cggtaatcag ggagctcatg
      481 cgtatctcga cgccggggcg ccgcgtctat gcttcggttc gcgacctgcg gccgcaccgc
      541 agcgggctcg gactttcaat cgtttccacg ccccagggcg tgatgaccga tacaagcgcg
      601 cgcgagaaga acgtgggcgg agaagtcatc tgccgcgtgt tctaggcggc caggaatgga
      661 gctgaattga tgtcccgtat aggcaaaagg ccggttcctc tgccccaagg cgtaagcgcc
      721 acggtcgagg gacaatccgt aagggtgaaa ggcccgaagg gtgagcttag cgtcatcctc
      781 gcgcatgacg tggaagcgag tgtcgccggg gaaggcgttg tcgtcgcgcc acggagcggc
      841 gccgagcgtg gccgcgaaat gtggggctta agccgaaccc tcgtgaacaa tctggtgacg
      901 ggagtgacgc aaggtttcac gcagaagctg gagatttccg gcgtcggata ccgcgcggcg
      961 gtgcaaggga agaatctaca gctgcaactt ggcttcagtc acgacgtagc gtacccgatt
     1021 ccgagcggga tcaatatcgt atgcgagaag ccgacgatga tcaccgtttc gggcatcgac
     1081 aagcagttgg tgggccaggt ggcggcggag atccgcgcct ggcggccgcc cgaaccctac
     1141 aagggcaagg gcgtgaaata cgccggggac cgaattcggc gtaaggaagg caagaagaaa
     1201 tagccatggc aagcgggaaa tctctcttcg accggcgcaa gcgacgtacg cgcttccggg
     1261 tcatgcgggc ggccgccggc aggccacgat tgtccgtgtt ccgctcgggt cggcatatct
     1321 acgctcagct catcgacgac cgggcaggcc aaacgatcgt cactgcgtcg acgaacgaga
     1381 aggaaggcaa ggcgcccaag acgtggaata ttgaggcggc aaccggcgtg ggcaaaatga
     1441 tcgccgagcg cgcgcttgcc aagggcgtca agcaggttgt gttcgatcgg ggcggctata
     1501 tctatcacgg gcgcgtcaag gcgctggcgg aggccgcacg cgcaggcggc ctcgaatttt
     1561 gagcgaagga ttgacagatg gcgcgtgaac agaggcagcg cgaagaacgc gacagcgagt
     1621 tcgtcgaccg gctggtgcac atcaatcgcg ttgcaaaggt tgtcaaagga ggccggcgct
     1681 tcggttttgc cgcgctcgtc gtcgtcggcg atcagcgtgg ccgagtcgga tttgggcacg
     1741 gcaaggcgcg cgaagtgccg gaggcgatcc gcaaggccac cgaggcggcc aagcgcgcga
     1801 tgatccgcgt gcccctcaag gacggccgca cgctgcatca cgaaacgcgg ggtcaccatg
     1861 gtgcaggcaa ggtgctcgtt cggccggcgc cggcgggcac gggaatcatt gctggggggc
     1921 ctatgcgcgc cgtgttcgag tcgctcggtg tcggcgatgt cgtcgcaaaa tcgctcggca
     1981 cgtcgaatcc ctataatatg gtgcgggcga cgttcgatgc gctcaagctg caacagtcgc
     2041 cgcgcatggt ggcgcagcgg cgcggccgca gtgtcagcga gatcatcgcg cggcgtgaag
     2101 gcgcgccgaa ggccgagcag gcggcggcgg ggagctgaag atggctgaga aaagcaaaag
     2161 cacgctcaag gtgagacaga ttcgcagcgc aaatcgccgg cctgagacac aggcggccac
     2221 gttacgtggg cttggcctgg gcaaaatcag gcgaacgcga acgcttgagg atacccccgc
     2281 ggtgcgcgga atgatccgcg ccgtgcaaaa cctcgtcgaa atcgttgagg aaagcggcaa
     2341 atgaaactca atcagcttgc caacaacgaa ggcgcacacc ggcggaagac gaaggtcggg
     2401 cgtggatcgt cgtcgggcct cggcaagacc tgtgggcgcg gcgtcaaagg cgccaaggcg
     2461 cgcaccggca ccaaggtcta tggtttcgag ggcggccaga tgccgctcta catgcgtctg
     2521 ccaaagcgcg gcttcaacaa catcttcgca aatgattttt ccgaagtagg ccttgcacgc
     2581 attcaaaagg cgatcgacga caagaaactc gaagcgtcca ggaagattac cgagggcgcg
     2641 ctccggaaag cgggtcttgc gcggtcgagt cgcaatgggg ttcggttgct gggcggcgcg
     2701 ctcacgtcga cggtcgagat cgaagttgca ggtgcatctg cggcagcggt cgagcggatc
     2761 cgaaaggccg gcggctccgt gacgctgacg ttcaggaaga ccgttcgaac ggacaagaaa
     2821 ggtcagcccg gcaaaaaggc gcagcgtcgg gcgaaggctg cggaaaaaag ggccgcgcgc
     2881 gccagctagc ttgccgatct tcggcgaggc ggcaggcagg actgggttca ggagtttctc
     2941 atggcctctg cggccgaaca gctggcagca aacttcaact tctccaattt cggcaaggcc
     3001 aaggagctgc gtcagcgcat cttcttcacg cttgcgctgc tcgtggtggc gcggctgggt
     3061 accttcattc caatgcctgg catcgatccg agcgagcttg cccgccagct cagccagcaa
     3121 cagggcggcg ttctcgacat cttcaacatg ctgtcgggcg gcgccgtcca gcgcatggcc
     3181 atcttcgcgc tcggcatcat gccatacatc tcggcctcga tcatcattca gctcatgacg
     3241 acggtatttc cggagctgga
//
LOCUS       DASZMC010000141         4970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_340443,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000141 DASZMC010000000
VERSION     DASZMC010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340443"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3734)
                     /locus_tag="VG274_02820"
     CDS             complement(<1..3734)
                     /locus_tag="VG274_02820"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005736.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWE05612.1"
                     /translation="MRIRRHFTSEGHSAFEGIAFRAATSEIRNPDGSVVFRQADIEVP
                     AEWSQVACDVLAQKYFRKAGIPVRLKPVAEEGVPEFLWRHVADGEERTGETSARQVFC
                     RMAGAWTYWGWKGGYFDSEDDARAFQDELCHMLAAQKAAPNSPQWFNTGLHWAYGIDG
                     PSQGHYYVDHLSGRLVKSTSAYEHPQPHACFIQSVSDDLVNEGGIMDLWTREARLFKY
                     GSGTGTNFSGLRGENEKLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVIVDV
                     DHPDIEQFIDWKVVEEQKVAALVAGSKLAERHLKAVMRACINCEGSGDDCFDPKKNPA
                     LRREIKAARKSMIPDNYIERAIAFARQGFKEIDFRIPTYDTDWDSEAYLSVSGQNSNN
                     TVRVTDAFLKAVEDDEDWRLTYRKGGIAEEMPARALWDKINYAAWACADPGIQFHTSI
                     NDWHTCPAGGEIRASNPCSEYMFLDDTACNLASLNLMAFRKASESGKRFDVSAFEHAV
                     KLWTVVLEISVMMAQFPSREIAQRSFDYRTLGLGFANIGGYLMAAGIPYDSREGRAIA
                     GAISAVMTGVSYATSSELAGELGPFPEYGANAEAMLRVIRNHRRAAHGATDGYEQLAT
                     VPVPLDLAALTDELLAAAAARAWGDALAFGEEFGFRNAQATVIAPTGTIGLVMDCDTT
                     GVEPDFALVKFKKLAGGGYFKIINRCVPEALSALGYQQSEIDDIVAYAVGNGTLETLT
                     YGVTHDALRAKGFGDETLATIESALEAAFDIRFVFNKWMLGERFCTETLGLAAAQLDA
                     PEFDMLKALGFSRSEIDTANLHVCGAMTLEGAPHLSSEHLAVFDCATPCGRIGTRALS
                     VESHIRMLAAVQPFISGAISKTINMPNSASVRDCGDAYMLSWKLGLKANALYRDGSKL
                     SQPLAAQVFNLADDDDALDDVLAAPLAARTSMVSERIVEKIVERVVSRERERLPQRRK
                     GYTQKAVVGGHKVYLRTGEYEDGRPGEIFIDMHKEGAAFRSLMNNFAIAISIGLQYGV
                     PLEEFVEAFTFTRFEPAGLVIGNDSIKNSTSVLDYIFRELAVSYLGRDDLAHVPPTHD
                     EDLGHGARGGNERREPARDLAQTLSRVASTGFMRGQLKSLTVVRSSNTVARAAPAHER
                     DAYADTADGFVNLIARPDPVTDALSEVRMAVMEAAGANPVGQAVARLEARLTLGDDAR
                     AYRAAEARMKGYEGDACGACGNFTLVRNGTCMKCDTCGST"
     gene            4163..4552
                     /locus_tag="VG274_02825"
     CDS             4163..4552
                     /locus_tag="VG274_02825"
                     /inference="COORDINATES: protein motif:HMM:NF006040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HWE05613.1"
                     /translation="MNGMSWLRAIFVWWQRATPGTLLLTWLSGHFVGEDKFGNRYYQN
                     RAALRRWVIYNGTVEASRVPAEWHGWLHHTFREPPTVAPLRVRPWEKEHVPNLTGTDM
                     AYRPPGSLAASGQRAPATGDYRAWKPE"
     gene            4577..>4970
                     /locus_tag="VG274_02830"
     CDS             4577..>4970
                     /locus_tag="VG274_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HWE05614.1"
                     /translation="MQNNTFETLMGAVVILVAAGFLYFAYTSTSVGSLGGYELGARFA
                     SADGIATGTDVRLHGIKIGRVSSLELDPKTYAAIVKITVRDDVKLPDDSSIKITSAGI
                     MGSSYLAIQPGGSEKMIPPGGQITNTQGS"
BASE COUNT          907 a         1548 c         1573 g          942 t
ORIGIN      
        1 ctggtgctgc cgcacgtgtc gcatttcatg caggtgccgt tgcggacgag ggtgaagttt
       61 ccgcatgcgc cgcaggcgtc gccctcgtaa cccttcatgc gcgcttccgc ggcgcggtac
      121 gcccgcgcgt cgtcgccgag cgtcagtcgc gcttccagcc gggcgaccgc ctgaccgaca
      181 ggattcgcgc ccgccgcttc catgaccgcc atccgcacct cgctcaacgc atccgtgacg
      241 ggatctggcc tggcaatcaa atttacgaat ccatctgccg tatctgcgta agcgtcgcgc
      301 tcgtgggcgg gcgccgccct tgcgacagtg ttgctgctgc gcacgacggt cagagatttg
      361 agctgtccgc gcatgaagcc ggtggaggca acccgcgaca gggtctgcgc aagatccctg
      421 gcgggctcgc gccgttcgtt gcctccgcgt gcgccgtggc ccagatcctc gtcatgcgtt
      481 ggcggcacat gcgcgaggtc gtcgcggcca agatacgaga ccgcaagctc gcggaagatg
      541 taatcgagca cgctggtcga gttcttgatc gaatcattgc cgatgacgag accagcgggc
      601 tcgaagcgcg tgaaggtgaa agcttcgacg aattcctcga gcggcacgcc atattgcagg
      661 ccgatggaaa tggcgatggc gaaattgttc atcaggctgc ggaaggccgc accttccttg
      721 tgcatgtcga tgaatatctc gcccgggcgg ccatcctcgt attcgccggt ccgcagatag
      781 accttgtgtc cgccgacaac cgctttttgg gtgtagccct tgcggcgctg cggcagcctt
      841 tcgcgttccc ggctgacgac gcgctcgacg atcttctcca caatgcgctc tgataccatc
      901 gaagtccggg cggcgagcgg cgcggcgagg acgtcatcga gcgcgtcatc atcgtcggcg
      961 agattgaaaa cctgcgcggc cagcggctgc gaaagcttcg aaccatcccg atagagcgcg
     1021 ttggccttca ggccgagttt ccaggacagc atgtacgcat cgccgcagtc gcgcacgctg
     1081 gcgctgttgg gcatgttgat ggtcttggag atggcgccgg aaatgaaggg ctgcacggcg
     1141 gccagcatgc gtatgtgact ctccaccgaa agggcgcgcg tgccgatccg tccgcacggg
     1201 gtggcgcaat cgaaaacggc gagatgttcg ctgctgagat ggggagcgcc ttcgagagtc
     1261 attgcgccgc agacatgcag attcgccgtg tcgatctcgg atctggaaaa accgagcgcc
     1321 ttcagcatgt cgaattccgg cgcgtcgagt tgcgccgctg ccaggccgag cgtttcggtg
     1381 cagaaccgct cgccgagcat ccatttgttg aagacgaagc ggatgtcgaa cgcggcctcg
     1441 agcgcggatt cgatggtggc gagcgtctcg tcgccgaatc ccttggcccg cagggcgtcg
     1501 tgcgtcacgc cgtaggtgag cgtttcgagg gtgccgttgc caacggcata ggcgacaatg
     1561 tcgtcgatct cgctctgttg gtagccgagc gccgaaagcg cttcgggcac gcagcggttg
     1621 atgatcttga aataaccgcc gccagccagc ttcttgaact tcaccagcgc gaaatccggc
     1681 tcgacgccgg tggtgtcgca atccatcaca agcccgattg tgccggtcgg cgcgatgacc
     1741 gtcgcctggg cattgcgaaa gccgaattcc tcgccgaagg cgagagcgtc gccccacgcg
     1801 cgcgcagctg ccgccgcgag caactcgtcg gtcaacgcgg caagatccag gggcacgggc
     1861 accgtcgcaa gctgctcgta tccgtcggtt gcaccatgcg cagctcgccg atggttgcgg
     1921 atcacccgca gcatggcttc cgcattggcg ccgtattccg gaaacggtcc gagttcgcct
     1981 gcgagctcgg acgaggtggc ataggacacg cccgtcatca ccgcggagat tgcgcccgca
     2041 atggcgcgac cttcgcggct gtcataggga atgccggccg ccatcagata cccgccaata
     2101 ttcgcgaagc cgagaccgag cgtgcggtag tcgaaagagc gttgcgcaat ctcgcgcgac
     2161 gggaactgcg ccatcatcac cgagatttcg aggacgaccg tccacagttt gaccgcgtgc
     2221 tcaaaagcgg agacgtcaaa acgtttcccg gactccgacg ccttccggaa cgccatcagg
     2281 ttgagcgagg cgaggttgca ggccgtgtcg tccaggaaca tgtattccga gcaggggttc
     2341 gaggcgcgaa tctcgccgcc cgccggacag gtgtgccagt cgttgatcga ggtgtggaac
     2401 tggatacccg gatcagcgca ggcccaggcc gcatagttta tcttgtccca cagcgcgcgc
     2461 gccggcatct cctcggcgat gccgcctttg cgataggtca gccgccagtc ctcgtcgtct
     2521 tcgaccgcct tcaggaacgc gtcggtgaca cggacggtat tgttggaatt ctgtcctgaa
     2581 accgagagat aggcttcaga gtcccagtcc gtgtcgtagg tcggaatccg gaaatcgatc
     2641 tccttgaatc cctgacgcgc gaaggcgatg gcacgctcga tgtaattgtc gggaatcatc
     2701 gatttgcgtg cggcttttat ttcgcggcgc aatgcaggat tctttttggg atcgaagcaa
     2761 tcgtcgcctg agccttcgca attgatgcag gcgcgcatga ccgccttcag atgccgctct
     2821 gcgagcttcg agccggcgac gagcgcggcg accttctgtt cttcgaccac tttccagtcg
     2881 atgaactgct cgatgtcggg atggtcgaca tcgacgatga ccattttggc cgcgcgccgc
     2941 gtcgtgccgc ccgatttgat cgcgccggcg gcccgatcgc caatcttcag aaagctcatc
     3001 agaccggaag atttgcctcc gccggaaagc ttctcgttct cgccgcgcag gccggagaag
     3061 ttcgtgcccg tacccgaacc gtatttgaag agacgcgcct cgcgcgtcca caaatccatg
     3121 atgccgcctt cgttgacaag atcgtccgag acgctctgga tgaagcaggc gtgcggttgg
     3181 ggatgctcgt aagccgaggt ggatttcacc agccggccag agagatggtc gacgtaataa
     3241 tgcccctggc tcgggccgtc gattccgtag gcccaatgca ggccggtgtt gaaccattgc
     3301 gggctgttgg gcgccgcctt ttgcgcagcg agcatgtggc acaattcgtc ctggaatgcc
     3361 cgcgcgtcgt cttccgaatc gaaataaccg cccttccatc cccagtaggt ccaggcgccc
     3421 gccatgcggc agaacacctg cctggcggag gtttcacccg tgcgctcctc gccgtcggcg
     3481 acgtggcgcc agagaaattc ggggacacct tcctcggcga cgggcttgag acggaccgga
     3541 attcccgcct tgcggaaata tttctgcgcc agtacgtcgc aggcgacctg gctccactcg
     3601 gccggcactt cgatgtctgc ctggcggaac accacggaac catccggatt gcggatctcg
     3661 ctggtcgcgg cgcgaaacgc gattccctcg aaagcggaat gaccttctga cgtgaagtga
     3721 cggcgaatgc gcatcttttc ccccggcccg gttccattac gatcgcgtcg ccgctgcgcc
     3781 tcggggatga ctctgcgccg tcggacacca gcctttcggc tggcggccac cgcatcgggc
     3841 gccacgctcc ttcccagatt ttgtgccgcg gcgaggcggc agaccccaag tatggacaca
     3901 cgttgccggc gcacgcaaga gacaagtgat aaacgagcgt ctacccacaa catcttgggc
     3961 ctcggcggga tggcggaatt gctcgcagat tcggcatgtc aaggagaatg gcggcgaccc
     4021 gccggaaaat attttcaacc ggaccgaccg tcgtggggcc attgcgccgg agagcgcgcg
     4081 aagccgccga tcggacctcc tcgtcgactg gcattggcgc gctcccgcgc caactactat
     4141 agtcgccgag atcttggatt cggtgaacgg catgtcgtgg ttgcgggcaa tattcgtatg
     4201 gtggcagcgg gcgacgccgg gcacattgct gctgacatgg ctgtcggggc atttcgtcgg
     4261 agaggacaag ttcggcaacc gctactacca gaaccgggcg gccttgcggc gctgggtgat
     4321 ctacaacgga accgtcgaag cctcgcgggt tcccgcggaa tggcatggct ggctgcatca
     4381 tacattccgc gagccgccga cggtcgcacc gctgcgtgtg aggccgtggg agaaggagca
     4441 cgttccgaac ctgacgggga ccgacatggc ctatcgtccc cccggcagcc tggctgcgtc
     4501 aggacagcgc gcgccggcga cgggcgacta ccgcgcctgg aaacccgagt gaaaatcagc
     4561 cggaggttat cgaaccatgc agaacaacac ctttgagacg ctgatgggcg cggtggtcat
     4621 cctggtggcc gcgggctttc tctatttcgc ctatacttcg accagcgtcg gatcgctggg
     4681 cggctacgag ctcggcgcgc gcttcgccag cgccgatggc atcgccaccg gaaccgatgt
     4741 gcgcctgcac ggcatcaaga tcggacgcgt gtcgtcgctg gagcttgatc cgaagactta
     4801 tgctgcgatc gtcaagatta ccgtgcgcga cgatgtgaag cttcccgacg attcctcgat
     4861 caagatcact tccgcgggca tcatgggaag ctcctatctc gccattcagc cgggcggcag
     4921 cgaaaaaatg attcctccag gggggcaaat cacgaatacg cagggatcga
//
LOCUS       DASZMC010000142         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_56612,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000142 DASZMC010000000
VERSION     DASZMC010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56612"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VG274_02835"
     CDS             complement(<1..972)
                     /locus_tag="VG274_02835"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWE05615.1"
                     /translation="MPSLNTLAPDRLHPMEARLVSELPKKGRWQFEPKWDGFRCLAFR
                     DGAKVELRAKSGKSLTRYFPEIAVALAALDARKLVLDGELLIPEGKTLSFDALQMRLH
                     PAQSRIEKLSRKTPAIYMLFDLLANAEGISLLDQTLVERRKKLECFHARIEGCDRFRL
                     SPFTTRIGEARKWLARAGGGALDGVIAKPLDEPYRPGERAMLKVKCLRTADCVVGGFR
                     FTSGSHDIGSLLLGLYDDEGKLDHIGFTSAIPERDQPALRKKLAPLRGGPGFTGNAPG
                     GPSRWSTERSSQWEPLKPVLVAEVRYDHVTGRRFRHGARFLRWRPDKK"
     gene            complement(1171..2130)
                     /locus_tag="VG274_02840"
     CDS             complement(1171..2130)
                     /locus_tag="VG274_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWE05616.1"
                     /translation="MAETACHALPTQDRIEPTESRALRVLVIAGSARRLNSCPGLDSK
                     ARALMHRMIGKLPAGWQTDHEDIGNEHGKPRIQACNACVSSSMALCVWPCNCYGPRSE
                     TQPDLMWNLDLYPRLARADAWAIIGPVCWYGPSTNLKTMFDRLVCMNGGNPRPDLIDK
                     KNTLKAQALERSPLWKELSKNHLSGRTAAFFCYGDDGANEEDERGVPKKLEHPAWFDA
                     EKHPYRHSSEGEHLTYQGLVWQCRYSDIEVPDRLWRHATFGAGRPYADDQADDMMKEK
                     EGLAVFDEWVADFASHVQAKGAIPATDESERNARSTTNRGKDS"
BASE COUNT          433 a          734 c          753 g          498 t
ORIGIN      
        1 ctttttgtcg ggccgccagc gcaggaatct ggcgccgtgc cgaaaccgtc ttccggtgac
       61 atggtcgtat cgcacttcgg caacgagcac aggtttcagc ggctcccatt gcgaactgcg
      121 ctccgtcgac catcggctcg ggccgcccgg cgcattgccg gtgaagcccg gtccgccgcg
      181 caggggcgca agcttcttcc tgagcgcagg ctgatctctt tcggggattg cggacgtgaa
      241 gccgatatga tcgagctttc cctcatcgtc atagaggccg agcagaagcg aaccgatatc
      301 gtggcttccg ctcgtgaaac ggaagccgcc gacgacgcaa tcggcggtgc gcaggcattt
      361 caccttcaac attgcccgtt cgcccggcct gtatggttcg tcgagcggct tggcgatgac
      421 gccgtcgagc gcgcctccgc cggcgcgggc gagccatttc ctggcctcgc cgatccgcgt
      481 cgtgaacggt gaaagacgaa agcggtcgca tccctcgatg cgggcatgaa agcactcaag
      541 ttttttgcgc cgttcgacga gtgtctggtc gagcaaggag attccttccg cgtttgcgag
      601 caggtcgaat agcatgtaga tcgccggcgt cttcctcgac agcttctcga tgcggctctg
      661 tgcggggtgc aaccgcatct gcagcgcgtc gaaagacaat gttttgcctt cgggaatgag
      721 aagctcgcca tcgagaacca acttccttgc gtcgagcgcc gcgagcgcca cggcgatctc
      781 cggaaagtac cgcgtcagcg attttccgga tttggcgcgc aactcgactt ttgccccgtc
      841 gcgaaaggcg agacagcgga atccgtccca tttcggctcg aactgccagc ggcccttctt
      901 cggcaattcg gagacgagcc ttgcttccat gggatgcagc cggtcgggag cgagtgtgtt
      961 cagcgagggc atgagcacgg agccttgata tcaacaaggc ggcacgcgga gacgcggaag
     1021 aaaaagggca tcacaatgag acaatgaggc agtgagagag acagacaatg agaaccgaat
     1081 tgcgcgcctt tggcacgcaa acattctcgc tgtctcatcg tctcattgtg aatctttcgt
     1141 gttcccgcgc ctccgcgtgc cgcctttttg tcagctgtct tttccccggt tcgttgtcga
     1201 tcgggcgttg cgttcgcttt catccgtggc cggaattgcg ccttttgctt gcacatggga
     1261 cgcaaaatcg gcgacccatt cgtcgaacac ggcgagtcct tccttttcct tcatcatgtc
     1321 gtcggcctgg tcgtcggcgt agggccgccc cgcgccgaag gtggcatggc gccacaggcg
     1381 gtcgggcact tcgatatcgc tgtagcggca ttgccagacg aggccctgat aggtgaggtg
     1441 ctcgccttcg ctgctgtggc ggtagggatg tttttcggca tcgaaccatg ccggatgttc
     1501 gagcttcttc ggcaccccgc gttcgtcttc ctcgttggcg ccgtcgtcgc cataacagaa
     1561 gaacgccgcc gtgcgccccg agagatgatt cttgctcagc tccttccaaa gcggcgagcg
     1621 ctcgagcgcc tgtgctttca acgtattttt cttgtcgatc agatcgggac gcggattgcc
     1681 gccgttcatg cagacgagac ggtcgaacat ggtcttgaga ttggtcgaag ggccgtacca
     1741 gcacaccggt ccgatgatcg cccaggcatc ggcgcgcgcg agacgcggat agagatcgag
     1801 attccacatc agatccggct gtgtctcgct tcgcggaccg tagcaattgc agggccagac
     1861 gcagagcgcc atcgaagaac taacgcaggc gttgcaggcc tggatgcgcg gcttgccgtg
     1921 ctcgtttccg atatcctcgt gatcggtttg ccagcctgcg ggcagcttgc cgatcatgcg
     1981 atgcatcagc gcgcgcgcct tggaatccag acccggacag gaattgagcc gccgcgccga
     2041 gccggcgatc accagaacgc gcagagcccg cgattccgtc ggttcgatcc ggtcctgcgt
     2101 cggcagggcg tggcaggcgg tttcggccaa ttcagctccg gagcagttct tcggggccgg
     2161 cgaaaaatcc cttgccggcc caatccgcaa cgcgtcgcga attcacgcgg ttccttgacc
     2221 cgtcattgca ggctgggaat acgccgcgct cgcggctgtt cttcgcatgt gcggagccaa
     2281 aacgcatgag tgagcaataa ggacgtaacg gggccgaaat cgagcgcgcg gatctggtca
     2341 cgcacgattc gatccgtcgt ctcgcacggt ataggctgcc gcgcgccgag cgagggaggg
     2401 atatatggat tcgcccgc
//
LOCUS       DASZMC010000143         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1717006,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000143 DASZMC010000000
VERSION     DASZMC010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717006"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2415)
                     /locus_tag="VG274_02845"
     CDS             complement(<1..2415)
                     /locus_tag="VG274_02845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HWE05617.1"
                     /translation="MSASFAEIAVTTNFSFLRGASHPKELAAAAALQGHAAIGIADRN
                     TLAGIVRAYEGLNEVREKTGETTKLLVGARLVFVDGTPDILAYPTDRPAYGRLCRLLS
                     AGKLRAAKGECILTLADLIEWQDGFLLVVMPPHPAREEASGDPFRKSWNTEDVASAPR
                     LSSPIVGEVPSAARRRGASRAATPPSGPSGHLPHDGEGKLSVVADAVTSAHFLTLLET
                     LSSVAPDRVWLGASTLYRGDDARRLRKLARIAHSVGVPLIATNDVLYHAPERRALQDI
                     VTCIREHVTLDEAGRLLEPNAERHLKPPEEMAALFREAPEAIAETIRFAECIQFSLDQ
                     IKYNYPDEPVPKGKTAQQHLEDLSWDGANKHYPGGVPGKVRASIEKELALIATLDIAP
                     YFLTVNDIVGFAKSRGILCQGRGSAANSAVCYALGITSVDPMQIDLLFERFVSQERRE
                     PPDIDVDFEHERREDIIQDVYKRYGAHRAALTATVICYRPRSAIREVGKVFGLTEDVT
                     AALASGVWGSWGSGLKESEVRQGRLDPANALVARAVGGANALICFPRHLSQHVGGFVL
                     TRDRLDEIVPIGPAAMENRYFIEWDKDDIDTLMMMKVDVLALGMLTCIRKAFELIAAH
                     EGRRYTLASLPREDPQVYEMLCKADAVGVFQVESRAQMNMLPRLAPKEFYDLVIEVAI
                     VRPGPIQGDMVHPYLRRRSGREPVDYPSPSPEHGPPDELQTVLRKTLGVPLFQEQAMK
                     LAIVAAGFTPGEANGLRRAMATFRNVGTIDQFRQKMIMGMTARGYAQDFAERCFKQIE
                     GFGSYGF"
     gene            complement(2412..>3155)
                     /locus_tag="VG274_02850"
     CDS             complement(2412..>3155)
                     /locus_tag="VG274_02850"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HWE05618.1"
                     /translation="SLSGLLTERGEGARRMEAVFFRADGAVHRLAVETGGPTRDPAII
                     ARLFRERLDALADPLDPGFGFDLIRLSADRAERCEAATSPFDARANEEKEIAFLIDRL
                     AARFGSQRILSFRPNDTHIPEAASVALPAQSVENSALPWKAIRTRGEAPRRPLRLFAR
                     PQPIVAIAAVPDGPPLRFRWRRALHEVALAEGPERIAMEWWRHQTPQPTRDYYRVEDA
                     KGRRFWLYRDGLYHRETDSPRWFLHGLFA"
BASE COUNT          543 a         1001 c         1064 g          547 t
ORIGIN      
        1 aaaaccgtag ctgccgaagc cttcgatctg cttgaagcag cgctctgcga aatcctgcgc
       61 gtagccgcgt gctgtcatgc ccatgatcat cttctgacgg aactggtcga tggtgccgac
      121 attgcggaac gtcgccatcg cccggcgcaa cccgttggcc tctcccggcg tgaagcccgc
      181 cgccacgatg gcgagcttca tcgcctgctc ttggaacagc ggcacgccca gcgtcttcct
      241 gagcaccgtc tgcaattcgt cgggcggccc gtgttcgggc gagggtgagg ggtaatcgac
      301 cggttcgcgg ccgctgcgac gcctgagata gggatggacc atgtcgccct ggatgggccc
      361 cggccgcacg atcgccacct cgatgacgag atcgtagaac tccttgggcg ccagccgcgg
      421 cagcatgttc atctgcgcgc ggctttcgac ctggaacacg ccgaccgcat cggccttgca
      481 cagcatttcg taaacctggg gatcttcgcg cggcaggctc gccagcgtat agcgccggcc
      541 ttcatgcgcg gcgataagtt cgaaggcctt gcggatgcag gtgagcatgc cgagcgccag
      601 cacatccacc ttcatcatca tcagcgtatc gatgtcgtcc ttgtcccatt cgatgaagta
      661 gcggttctcc atcgcggcgg ggccgatggg cacgatctcg tcgagccggt cgcgcgtcag
      721 cacgaagccg cccacatgct gggagagatg ccgcggaaag cagatcagcg cattggcgcc
      781 gccgaccgcg cgcgcgacaa gcgcgtttgc gggatcgagg cgcccctgtc gcacctcgct
      841 ttccttcagg ccgctgcccc agcttcccca tacgccgctg gcgagcgccg ccgtcacgtc
      901 ttcggtgagg ccgaacacct tgccgacttc gcggatggcg ctgcgcgggc gatagcagat
      961 gaccgtcgcc gtcagcgcgg cgcgatgcgc gccataacgc ttgtagacat cctggatgat
     1021 gtcctcgcgc cgctcatgtt cgaaatcgac gtcgatgtcg ggcggctccc ggcgctcctg
     1081 cgaaacgaaa cgctcgaaca gaagatcgat ctgcatggga tcgacggaag tgatgccgag
     1141 cgcgtagcag acggcggagt tcgcagccga gccgcggccc tggcagagaa tgccgcggct
     1201 cttcgcaaac cccacaatgt cgttgaccgt caggaaatag ggcgcgatgt cgagcgtggc
     1261 gatgagcgca agctccttct cgatagaagc gcgcaccttc ccgggaacgc caccgggata
     1321 atgtttattc gcgccgtccc aggacagatc ttccagatgc tgctgcgcgg tcttgccttt
     1381 cggcaccggc tcgtcgggat aattgtattt gatctggtcg agcgagaatt ggatgcattc
     1441 ggcgaagcgg atcgtctcgg caatcgcttc gggggcctcg cggaacagcg ccgccatttc
     1501 ttcgggcggc ttcaggtggc gctcggcatt gggttcgagc aggcggccgg cttcgtcgag
     1561 cgtcacatgc tcgcggatgc aggtgacgat gtcctgcagc gcgcgccgct cgggcgcatg
     1621 atagagaacg tcgttggtcg cgatgagcgg aacgcccacg ctgtgcgcga tgcgcgcgag
     1681 ttttcgcagt cgtcgcgcat cgtcgccgcg atagagcgtg gatgcgccca gccacacgcg
     1741 atcgggcgct accgaggaaa gtgtttcgag gagtgtgagg aagtgagcgg aagtaacagc
     1801 atcagctaca acgcttagtt ttccttcccc atcgtggggg aggtggcccg aagggccgga
     1861 ggggggcgtg gctgcgcgcg atgcccccct ccgcctcgct gcgctcggca cctcccccac
     1921 gatgggggag gaaaggcgtg gtgcggaagc gacatcttca gtgttccacg atttccggaa
     1981 cggatctcca cttgcttcct cgcgtgcggg gtggggtggc atgacgacga gcagaaaccc
     2041 gtcctgccat tcgatgagat cggcaagggt gaggatgcat tcgcctttcg ccgcgcgcag
     2101 cttgcccgcg ctcagcagcc ggcagagccg gccataggcc ggccggtcgg tgggataggc
     2161 gaggatgtcg ggcgtgccgt cgacgaaaac cagccgcgcg ccgacgagca gtttcgtcgt
     2221 ctcgccggtc ttctcgcgaa cctcgttcag cccctcatag gcgcgcacga ttccggccag
     2281 cgtgttgcgg tcggcgatgc cgatggcggc gtggccctgg agcgccgccg ccgccgccag
     2341 ctccttcgga tgggacgcgc cgcgcaggaa cgaaaaattg gtggtgaccg cgatctcggc
     2401 gaaggaggcg ctcatgcgaa cagcccgtgc agaaaccagc gcggggaatc ggtctcgcgg
     2461 tggtaaagcc cgtcgcggta gagccagaag cgccggccct tcgcatcctc cacgcggtaa
     2521 tagtcgcgcg tcggctgcgg cgtctgatgg cgccaccatt ccatcgcgat gcgttcgggg
     2581 ccttcggcaa gcgccacttc gtgcagcgcg cgccgccagc ggaatctcaa cggcggtccg
     2641 tctggcactg cggcaatggc cacgatgggc tggggccgcg caaacagacg caatggccgg
     2701 cgcggagctt cgccgcgcgt gcggatggct ttccagggga gcgcggaatt ttcaacggac
     2761 tgcgcgggca acgcgaccga agccgcttcg gggatatgcg tgtcgttcgg ccggaaggac
     2821 agaatgcgtt gcgaaccgaa gcgcgcggcg agccggtcga tcaggaaagc gatctccttc
     2881 tcctcattcg cgcgcgcatc gaatggagaa gtcgccgcct cgcagcgttc ggcgcgatcg
     2941 gcagaaagac ggatgagatc gaagccgaat ccgggatcga gcggatcggc aagtgcatcc
     3001 agcctttcgc ggaagagccg cgcgataatc gcaggatcgc gcgtggggcc gcccgtttcg
     3061 accgcgaggc gatggacggc tccgtcggcg cggaaaaaaa ccgcttccat ccgccgcgcg
     3121 ccttcgccgc gttccgtgag caggccgctg agcga
//
LOCUS       DASZMC010000144         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_56985,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000144 DASZMC010000000
VERSION     DASZMC010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56985"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1989)
                     /locus_tag="VG274_02855"
     CDS             complement(169..1989)
                     /locus_tag="VG274_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWE05619.1"
                     /translation="MAQTGAEFASDVQRVAAARARSRSLRPLRDLLPYLMPYRWRIVT
                     ALVALLCSATVSLLVPQALRIMIDRGFHTADAARIGPYFLPLVIASGLLGIFTAVRFY
                     YVTWIGERVIADIRKAVFDHVLSLTPAFFEITRTGEVLSRLTADTTLIQTVIGSSVSV
                     AMRNAVMLVGGLALLFATSVKLTAIVLAAVLLVLLPLILFGRWVRALSRRSQDRIADT
                     SARASETINAVQTVQAFTQENAERGRFSRAVELSFDVSILRTRARAVMTAVVIFAIFS
                     SIVGVAYVGAHDVLAKTMTAGLLVQFIFYALIVGLGVGALSEIWGDLQRAAGASERLT
                     EILRVEPEVKVPLHPERVPQPVRGAIAFRDVTFRYPSRLEHAALDGFSLEVKLGEAIA
                     LVGPSGAGKTTVFQLALRFYAPQSGAIRFDGIDMARLDPAELRRHIGLVAQDPVIFSG
                     TIADNIRYGRPEASDAKVREAAEAAAAAEFIEMLPMGYQSLVGERGTTLSGGQRQRLA
                     IARAILRDAPLLLLDEATSALDAENERLVQMGLTNLMAGRTTIVIAHRLATIQRLKRI
                     VVMDEGRVLMEGSHAELVARGGLYARLADLQFTAGLARAG"
     gene            2153..2377
                     /gene="rpmE"
                     /locus_tag="VG274_02860"
     CDS             2153..2377
                     /gene="rpmE"
                     /locus_tag="VG274_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWE05620.1"
                     /translation="MKKDIHPNYHMVTVVLNGGSTYRTRSTWGEPGQKLTLDIDPSTH
                     PAWTGGQQQLVDRGGRLSRFNKRFKDYVKS"
     gene            complement(2396..2911)
                     /locus_tag="VG274_02865"
     CDS             complement(2396..2911)
                     /locus_tag="VG274_02865"
                     /inference="COORDINATES: protein motif:HMM:NF018972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HWE05621.1"
                     /translation="MSLAEAQKEKPRGVTRHASMDSMLFDRTFDEGMSLVEETARYLD
                     GRGREESRVLPRKAALLYAGESMRVTTRLMQAASWLLIQRAVREGEMRAEDAAGERYR
                     LGSKEICLGGRSEGIEYLPRTLRDLLDRSERLYRRIARLEKAMLGESPQIRPVVQVQF
                     ERLQQSFGEAV"
     gene            complement(3068..>3210)
                     /locus_tag="VG274_02870"
     CDS             complement(3068..>3210)
                     /locus_tag="VG274_02870"
                     /inference="COORDINATES: protein motif:HMM:NF018413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HWE05622.1"
                     /translation="ADLSATSEHELVARIAALETEIARCRDAIAARQSTRAAADSVFR
                     KS"
BASE COUNT          598 a         1049 c         1023 g          540 t
ORIGIN      
        1 tgccacgatg tccatgttgc gcaagaaaaa atcggcgagg acgctatgcg ttatgacgca
       61 gtaagattaa gcggcgtcaa gaacgacgcg tatcgacgcg gcgcattgca gatttcagct
      121 gggtggcccg ctcttcggcg ggcgacaatt gatttattca cgctcggtct accccgccct
      181 tgcgaggccg gcggtgaatt gcaggtcggc gaggcgggcg tagaggccgc cgcgcgcgac
      241 aagctcggcg tggctgcctt ccatcagcac gcggccttcg tccatgacga cgatgcgctt
      301 cagccgctgg atggtcgcga ggcgatgggc gatgacgatg gtcgtgcgcc ctgccatcag
      361 gtttgtcagc cccatctgca cgagtcgttc gttctcggcg tcgagcgcgc tcgttgcctc
      421 gtcgagcaga agcagcggcg cgtcgcggag aatggcgcgc gcgatcgcca ggcgctggcg
      481 ctggccgccc gaaagggtcg tgccgcgctc gccgaccagg ctttgatacc ccatcggcag
      541 catctcgatg aattccgcgg cagctgccgc ttccgccgcc tcgcgcactt tcgcgtcgct
      601 cgcctcgggg cggccgtagc ggatgttgtc ggcgatggtg cctgaaaaga tgacgggatc
      661 ctgggcgacg agcccgatgt ggcggcgcag ttccgccgga tcgagccgcg ccatatctat
      721 gccgtcgaag cgaatggcgc ccgattgcgg cgcatagaag cgcaaggcga gctgaaacac
      781 ggtggtcttt ccggcgcccg acggccccac cagcgcgatc gcctctccga gtttcacctc
      841 gagcgaaaaa ccgtcgagcg ccgcatgctc cagccgggac ggatagcgaa acgtgacatc
      901 gcggaacgca atcgcgccgc gcaccggctg cggcacacgt tcgggatgaa gcggcacttt
      961 cacctcgggc tcgacgcgca ggatttcggt gagccgctcg gaagcgccgg cggcgcgctg
     1021 cagatcgccc cagatctcgc tcaacgcacc cacgccaagg cccacaatca aagcgtagaa
     1081 gatgaattga accagcaggc ccgccgtcat ggtcttcgcc aacacgtcat gcgcgcccac
     1141 ataagcgacg cccacgatgc tcgagaaaat ggcgaagatc acaaccgccg tcatcaccgc
     1201 acgggcgcgg gtgcgcagga tcgatacgtc gaacgacagt tcgaccgcgc gggagaagcg
     1261 gccgcgctcg gcgttctcct gggtgaaagc ctggacggtc tgcaccgcgt tgatcgtctc
     1321 gctggcgcgc gcgctggtgt cggcgatgcg gtcctggctg cggcgcgaca gcgcgcgcac
     1381 ccagcggcca aacagaatga gcggcaacag gacgaggagc acggcggcga gcacgatcgc
     1441 cgttagcttg acgctggtgg cgaaaagcag cgcgagcccg ccgacgagca tgacggcgtt
     1501 gcgcatcgcc accgaaacag aggagccgat tacggtctgg atcagcgtcg tatccgcggt
     1561 cagccgcgac aggacttcgc ccgtgcgggt gatctcgaag aacgccggcg tgagcgacag
     1621 cacgtgatcg aacaccgcct tcctgatatc cgcgatcacc cgctcgccga tccaggtcac
     1681 gtagtagaag cggacggcgg tgaagatgcc gagcagcccg gaggcgatga cgagcggcag
     1741 gaaatagggg ccgatgcgcg ctgcatctgc ggtatggaat ccgcgatcga tcatgatgcg
     1801 cagggcctgc ggcaccagca ggctgacggt ggccgaacag agaagcgcga ccagcgccgt
     1861 cacgatccgc caccggtacg gcatgagata gggcagaagg tcgcgcagcg gcctgagcga
     1921 gcggcttctg gcccgcgccg cagccacccg ctggacgtcg cttgcgaatt ccgcgcctgt
     1981 ctgtgccatc cgcccggccc tgatccctgt ccctgtgaca cctaatattc cctgtgacac
     2041 ctaatatagt gtgcaagcgg ctcgcgggag cgggccgact gcggcgcctt gtcaggcggg
     2101 aaccgatcgc ctataagccg ccgcttcccg gaagattgcg agcagacggc ccatgaaaaa
     2161 ggacatccat cccaattatc acatggtgac ggtcgttttg aacggcggga gcacctacag
     2221 gacccgctcg acttggggag agcccgggca gaagctgacg ctcgacatcg atccgagcac
     2281 gcatcccgcc tggacgggag gacagcagca gctcgtcgac cgcggcggcc ggctttcccg
     2341 cttcaacaag cgcttcaagg attatgtgaa gagctgacgg gcgcctcccg ccagctcaga
     2401 ccgcttcgcc gaagctttgc tgcaggcgct cgaattgcac ctgcacgacc ggcctgatct
     2461 gcgggctttc gcccagcatt gccttttcga gccgcgcgat acgccgatag aggcgctcgc
     2521 tgcggtcgag gagatcgcgc aacgttcggg gcaggtactc gatgccttcg cttcgtccgc
     2581 cgaggcagat ctccttcgat cccagacggt agcgctcgcc ggctgcgtct tcggcccgca
     2641 tttcgccttc ccgcaccgcg cgctggatga gcagccagct tgccgcctgc atcaggcgcg
     2701 tggtcacgcg catgctctcg cccgcgtaga ggagtgcggc tttccgcggc aaaacacgcg
     2761 attcttctcg gccgcggcca tcgagatagc gcgccgtttc ctcgaccagc gacatgcctt
     2821 cgtcgaacgt acgatcgaaa agcatggaat ccatcgacgc atgtcgcgtc acgccgcgcg
     2881 gtttttcttt ttgcgcttct gcaagcgaca tttctccccc aatccccgtt cagcccgcgg
     2941 gcaagctagc aaactgctct tgcggcggaa agagcgccgc gcaattgctc cacaatttta
     3001 cgcgatcatg gttacgcgcg ggcagcaccc aacgccgtta acaattggcg cgcgactcgc
     3061 acaccattca ggatttgcga aaaaccgaat ctgccgcggc tctggtcgat tgccgtgcgg
     3121 caattgcgtc tcggcatcgc gcgatctccg tttcaagcgc cgcaatgcgc gcaacaagct
     3181 cgtgttccga tgtggccgag agatctgctc
//
LOCUS       DASZMC010000145         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1717160,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000145 DASZMC010000000
VERSION     DASZMC010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717160"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /gene="bfr"
                     /locus_tag="VG274_02875"
     CDS             complement(<1..441)
                     /gene="bfr"
                     /locus_tag="VG274_02875"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HWE05623.1"
                     /translation="MPGNDRRHAGRGGRREGAAAGGVVDAPGPSPRASLPGNPDVIRS
                     LNLVLKNELTAINQYFLHSRMLADWGLSVLAKAEYEQSLDEMKHADRLIRRILFLGGL
                     PNLQDLGKLLVGESVKDILDCDLRLEQRSLPELHSAAGLCERVGD"
     gene            complement(372..560)
                     /locus_tag="VG274_02880"
     CDS             complement(372..560)
                     /locus_tag="VG274_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWE05624.1"
                     /translation="MIVCVCNRLGEARIWAAIDRGAETPEQVYAGCGVERNCGRCQET
                     IEDMLDEADAVKARRQAA"
     gene            complement(696..899)
                     /locus_tag="VG274_02885"
     CDS             complement(696..899)
                     /locus_tag="VG274_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWE05625.1"
                     /translation="MPIGTVKFFNTTKGFGFIAPEGGGKDVFVHATAVEAAGMRSLAE
                     GQRVSFDIQPDARGAKAVNLKAA"
     gene            complement(1143..1598)
                     /locus_tag="VG274_02890"
     CDS             complement(1143..1598)
                     /locus_tag="VG274_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWE05626.1"
                     /translation="MLPVAFYLDDLVVGQRFTSGSHALDEEQIKSFARTYDPQPFHTD
                     SEAARNSLFGGLAASGWHTAAITMKLNVESGLPIMGGIIGSGGEIDWPRPTRPGDVLR
                     VESEIIEIVPSRSRPDRGKVTVLCRTLNQKDEVVQTFRAKLVVPRKPHR"
     gene            complement(1611..>2533)
                     /locus_tag="VG274_02895"
     CDS             complement(1611..>2533)
                     /locus_tag="VG274_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05627.1"
                     /translation="NGGWANGDAAYSGQNPADGTVITYFQKTRQVIGRLKIDVLDATG
                     KLVTTLPASNRKGINRVVWSMRDDPPQTPPGANLVFNAARGPRVPPGIYTVRLTKGSN
                     VYTMPLAIGLDRRATFTIADRKAQYETAKRVSGLFGRMSRLAAGIVQVRDGAKAHESG
                     LPESDALRQELDGLAGDADALRKKIVATKEGGAITGEERLREHMDYVYGAIMSVEDRP
                     TPYQIARVNVLEHELKDVENSFGDLMKGKLASANAGLKAKGLSEITVREAPASVGGGG
                     GVAGGRVAGSLIGLRLPDFGALRAETDERD"
BASE COUNT          452 a          842 c          745 g          494 t
ORIGIN      
        1 gtcgcccacc cgttcacaaa gtcccgccgc gctgtggagt tccggcagcg agcgctgctc
       61 gagacgcaga tcgcagtcga ggatgtcctt gacgctctcg cccacaagaa gctttcccaa
      121 atcctgaaga ttgggcagtc cgcccagaaa caggatccgc cggatcagcc ggtctgcatg
      181 tttcatctcg tcgagagact gttcgtattc cgccttggcg aggacggaga gtccccaatc
      241 cgcaagcatt cgcgaatgaa ggaaatattg gttgatggcg gtgagttcgt tcttcagaac
      301 gagattgaga gaccggatga catcggggtt gcccggcaga gatgcccggg gggaaggtcc
      361 tggcgcgtcg actacgccgc ctgccgccgc gccttcacgg cgtccgcctc gtccagcatg
      421 tcttcgatcg tttcctggca tcgaccgcaa ttcctttcca cgccgcaccc agcatagacc
      481 tgctccgggg tttccgcgcc acggtcgatc gccgcccaga ttcgagcctc tcccagccga
      541 ttgcacacgc atacgatcat gaacagccaa tacccgatac cagcccacgg gaatatggtc
      601 tagttgagaa tgattgtcaa tcgcgattgg taaactgggc gtaaagaacc cgcggggaaa
      661 gcttcactac cccgcgggcg ctacggggtg gggaatcaag cggccttcag attgacagcc
      721 ttggcgccac gggcgtccgg ctgaatgtcg aacgacacgc gctgtccctc cgccagcgaa
      781 cgcatgcctg ccgcttcgac agcggtcgca tgaacgaata cgtccttgcc gccgccctcc
      841 ggcgcgatga atccaaaacc cttcgtggtg ttgaaaaatt ttaccgttcc gatcggcatg
      901 acttctcctg acacttcgag ttacgcccga acacccgttc agactgcaca aacggattct
      961 tcatcaggga gaaggtcggc tgtctcgtcc aggcgcgcat tcgcggtcgg atctacaaca
     1021 gtgcgcgctt cgtctgacga acgccgccgt acgcgcgtcc tataacccga tgcgccgcgc
     1081 aaggcaagcg cgcggacctg ctccgccccg cgtcgcgcgc ggaatctccg aacgcggccg
     1141 cgtcagcggt gaggtttccg tggaacgacg agcttcgccc tgaatgtctg aacaacctca
     1201 tccttctggt tgagcgtccg gcacagaacc gtcacctttc cgcggtccgg ccgcgagcgc
     1261 gacggaacga tctcgatgat ttcgctttcg acacgcaaca cgtcgccggg gcgcgtcggt
     1321 ctcggccagt cgatctctcc gccactgccg atgatgccgc ccatgatcgg cagtccgctc
     1381 tcgacattca gcttcatggt gatcgcggcg gtatgccagc cgctcgcagc gagaccaccg
     1441 aataacgaat tgcgcgcagc ttcgctgtcg gtgtggaacg gctgcggatc gtaagtccgc
     1501 gcgaaggatt tgatctgttc ttcgtccagc gcgtgcgagc cgctcgtgaa gcgctgaccg
     1561 acgacaagat cgtcgagata aaacgccacc ggcagcacgc gtcctttcgt tcagtcgcgc
     1621 tcgtccgttt ccgcgcgaag cgcaccgaag tcgggcaggc gcagtccgat caagcttccg
     1681 gcgactcgtc cgcctgcaac gcctccgccg cctccgaccg acgccggcgc ttcgcgaacg
     1741 gtgatctccg agagcccttt tgccttcagg ccggcattcg ccgaggcgag cttgcctttc
     1801 atgagatcgc caaacgaatt ctcgacatcc ttgagctcgt gttcgagaac gttgacgcgc
     1861 gcgatctgat agggggtcgg ccgatcctcc accgacatga tggcgccgta cacatagtcc
     1921 atgtgctcgc gcaaccgttc ttcaccggtg atcgcgccgc cttccttcgt ggcgacgatc
     1981 ttcttgcgca gcgcatcggc gtctccggcc aatccgtcca gctcctgccg caaggcatcg
     2041 ctctcgggta gcccggactc gtgcgccttc gctccgtcgc gcacctgaac gatgcccgcg
     2101 gcaagccggc tcatgcgtcc gaacaacccc gacacgcgct tggcggtttc atattgcgcc
     2161 ttgcgatcgg cgatcgtgaa ggtcgcgcgg cggtcgagcc cgatcgcaag cggcattgtg
     2221 tagacgttgc tgcctttggt cagccggacc gtatagatgc cgggcggcac gcgcggtccg
     2281 cgcgcggcat tgaacacgag gttcgcgccc ggaggcgttt gcggcgggtc gtcgcgcatc
     2341 gaccacacga cacggttgat gcccttgcgg ttggacgccg gcaacgtcgt gaccagcttg
     2401 cccgttgcgt ccagcacgtc gatcttgaga cggccgatca cctggcgcgt cttctgaaaa
     2461 taggtgatga ccgtgccgtc ggccggattc tgccccgaat atgccgcatc gccgttggcc
     2521 cagcctccat tgg
//
LOCUS       DASZMC010000146         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2821248,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000146 DASZMC010000000
VERSION     DASZMC010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2821248"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VG274_02900"
     CDS             complement(<1..293)
                     /locus_tag="VG274_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWE05628.1"
                     /translation="MGARAAAHQALSETLRRKRPLDGGLDEAISARELTERDAGFARA
                     IANETMRRFGQLDDLVRRFVPRAPPPHRAGASLEILLAGACELLFLGVPAHAAV"
     gene            complement(323..1192)
                     /gene="htpX"
                     /locus_tag="VG274_02905"
     CDS             complement(323..1192)
                     /gene="htpX"
                     /locus_tag="VG274_02905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HWE05629.1"
                     /translation="MNTLRTGLLMAALTGLFLVVGFLIGGQAGMLIAFLFAAASNLFA
                     YWNSDKVVLSLYGAQPVDAQSAPDLVHLVGELSQKASLPMPKVYIVDNAQPNAFATGR
                     NPEHAAVCVTTGLLQRVDREELAGVLAHELSHVKNRDTLTMTLVATIAGAVAMLANFA
                     FFFGGRNRNPLGIVGILLVTLLAPIAAMLVQMAISRGREFEADRSGAELSGRPLWLAS
                     ALQRIDRPAAAIANPEADANPATAHMFIVNPLHGGMAGLFSSHPSTEERIARLEGMAR
                     AMGQETKKPGPWA"
     gene            1283..1492
                     /locus_tag="VG274_02910"
     CDS             1283..1492
                     /locus_tag="VG274_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HWE05630.1"
                     /translation="MTKTQKPPPGDAEIAARIRAAAERAKREAQARRAAEAAPVAHEK
                     EIGGQGGPEPTRYGDWERKGLISDF"
     gene            1586..2149
                     /locus_tag="VG274_02915"
     CDS             1586..2149
                     /locus_tag="VG274_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017532211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWE05631.1"
                     /translation="MFIQTESTPNPQTLKFLPGLEVMGEGAVADFPDRDSAARSRLAS
                     ALFEIPDVARVFFGSDFISVTKSDADWRHLKPAILGAIMDHFTRGLPIVDGSAEETGD
                     ERAYGEGDPEIVAQIRELIDTRVRPAVAGDGGDIIFKGFDGGSGIVFLHLRGSCAGCP
                     SSTMTLKNGIENMLRHYIPEVNAVEAI"
BASE COUNT          440 a          735 c          762 g          392 t
ORIGIN      
        1 accgccgcat gggccggcac gccgagaaac agaagctcgc acgcgcccgc caggagaatc
       61 tcgagcgaag cgccggcgcg atgaggcggg ggcgcgcgcg gaacaaagcg gcggacgaga
      121 tcgtcaagct gaccgaagcg gcgcatggtt tcattggcaa tggcgcgcgc gaagcccgca
      181 tcgcgctcgg ttagttcgcg cgcggagatg gcttcgtcga gtcccccatc gagaggtctt
      241 ttccggcgca gtgtttcgct gagtgcctga tgagccgccg cgcgtgcgcc cattcccggc
      301 aagcttcccg cggagggtaa actcatgccc aggggccggg ctttttcgtc tcctgtccca
      361 ttgcccgggc catcccttcg agccgcgcga tgcgttcttc cgtggacgga tgacttgaaa
      421 agaggcctgc cattccaccg tgcagcggat tgacgatgaa catgtgcgcg gtcgcgggat
      481 ttgcgtcggc ttcgggattg gcgatggcgg cggccggtct gtcgatgcgc tgaagcgcgg
      541 aggcgagcca gagcggccgg ccggacaact cggcgccgga acggtccgcc tcgaattccc
      601 ggccgcggct gatcgccatc tgcacaagca tggccgcgat cggcgccagc aacgtcacga
      661 gaaggattcc gacaattccg agcgggttgc gattgcggcc gccgaagaag aaggcaaaat
      721 tggcgagcat cgccaccgcg cccgcaatcg tcgccaccag cgtcatcgtc agcgtgtcgc
      781 ggttcttcac atggctcagt tcgtgcgcca acacgcccgc cagttcctcc cgatccacgc
      841 gttgcaggag gcccgtcgtc acgcacaccg cggcgtgctc cggattgcgg ccggtcgcaa
      901 acgcgttcgg ctgcgcattg tcgacgatgt agactttcgg catcggcagg gacgccttct
      961 gcgacagctc gcctacgaga tgcacgagat cgggcgcact ctgcgcgtcc accggctgcg
     1021 ctccgtagag cgacaacacc accttgtcgg aattccagta ggcgaagaga ttgctggccg
     1081 ccgcgaacag aaatgcgatc agcatgccgg cctggccgcc gatcaggaat ccgacgacga
     1141 gaaacagccc cgtgagggcg gccatcaaaa gaccggttct gagcgtgttc atcatggcga
     1201 attgtcttcc gttggcagcc agcctaagtg agagacgctg gccctgccgt gatttaagcg
     1261 gggccggcac ggcttcaagc acatgaccaa aacccaaaaa cccccgcctg gggacgcaga
     1321 aatcgccgcc cgcatccgcg ccgccgcgga acgcgccaag cgggaagcgc aagcaaggcg
     1381 cgcagccgaa gctgcgccgg ttgcgcacga aaaggaaatt ggtggtcaag gcggcccgga
     1441 accgacgcgc tatggcgatt gggagcgcaa gggcctgatc tccgatttct gacaaaggct
     1501 tgactgcggc gtgcgggcct tgatcgccca gcccgggcgg gccatgtaag gtgagatcga
     1561 aacgcgtaca gggtgctgcc gacgcatgtt catccagacc gaatccacac cgaatccgca
     1621 gaccctgaag tttctgccgg ggctcgaggt catgggcgag ggagccgtgg ctgattttcc
     1681 cgaccgggac tcggcggcgc ggtcgcgatt ggccagcgcg cttttcgaga ttccagacgt
     1741 ggcgcgcgtc ttcttcggaa gcgacttcat ctccgtgacg aaatccgatg cggattggcg
     1801 ccatctcaag cccgcgatcc tcggcgccat catggatcac ttcacgcggg gtctgccgat
     1861 tgtcgatgga agcgcggaag agacgggcga cgaaagagcc tatggcgaag gcgatcccga
     1921 gatcgtggcg cagatccggg agctgatcga tacgcgcgtc cgccccgcgg tcgcgggcga
     1981 tggcggcgac atcatcttca agggtttcga cggcggctcg ggaatcgtgt tcctgcacct
     2041 gcgcggatcc tgcgcaggat gcccgtcttc gacgatgacg ctgaagaacg ggattgaaaa
     2101 catgctccgg cattacattc cggaagtgaa cgccgtcgaa gcgatctgac ctcggattgc
     2161 gccggctgct gtgcgattct gcgttccgca tagcgattcg gggcccctgc catgccaata
     2221 tccgacgacg cgctggacac catctttcgc aaggcgcgaa cgcacaacct ctggcaggac
     2281 aagccggtca gtccggcgct gctcatggcc gtctacgatc tgatgcgct
//
LOCUS       DASZMC010000147         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4454020,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000147 DASZMC010000000
VERSION     DASZMC010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4454020"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VG274_02920"
     CDS             <1..535
                     /locus_tag="VG274_02920"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWE05632.1"
                     /translation="TSPTREARGGGKPNAGASGLAGFMRRNICERMPSPSQKKGHMSR
                     YTALVDGKAGAYGVVFPELDGCAAMGTTIDEALANAAEALRDWVEVTEEHGEHVPSPQ
                     PLEKLRRRRQVMEALAEGAVLATVPLVRETGRPAKANLSLDSGVLAAIDEEAARRKLT
                     RSAFIEFLAPELLPKVA"
     gene            complement(546..1172)
                     /gene="mobA"
                     /locus_tag="VG274_02925"
     CDS             complement(546..1172)
                     /gene="mobA"
                     /locus_tag="VG274_02925"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HWE05633.1"
                     /translation="MTANRILGVILAGGASRRFGGDKSAALLAGRPLLERVVERARPQ
                     VETLLLNANEPDIGRSIPDLERLADSAPGEGPLAGILTVLGRAGRRGFDHVASFACDT
                     PFFPLDTVSRLAHALQASSADFAIARCGATAHRIFALWPVACRDQLDAAFAQGTRSMR
                     EVEDWLVPAWADFAAEGGPDSDPFFNINTPADLEAAERWLSQRGLCTN"
     gene            1317..1574
                     /locus_tag="VG274_02930"
     CDS             1317..1574
                     /locus_tag="VG274_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWE05634.1"
                     /translation="MRISVSDAKGQLTELVRRAEQGDDIVLTRHGQAVARVVPIRTVP
                     DRAARRKLLEAAQASGARKARRGPTAARSQDFLYDKNGLPK"
     gene            1571..1954
                     /locus_tag="VG274_02935"
     CDS             1571..1954
                     /locus_tag="VG274_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05635.1"
                     /translation="MIAVDTSALMAIILGEGAGNACMDALETETDVLISAGTAAESLI
                     VAARRRVADEMGSLIEGLGIEIVPVTMSSALRIADAYARWGKGAHPAALNFGDCFAYA
                     LAKEHSCRLLYVGDDFARTDVDRVL"
     gene            2212..2679
                     /locus_tag="VG274_02940"
     CDS             2212..2679
                     /locus_tag="VG274_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWE05636.1"
                     /translation="MKLRVNGTMHDVAADPSTPLLYVLRNDLGLNGAKFGCGLGQCGA
                     CTVLAGKDAIFSCITPIGSLANRDIRTIEGLGTMEHPGPLQRAFIDEQAAQCGYCTAG
                     IVMRAQALLETNASPTEEEIRRHMRPNLCRCGTQLRVIRAIRRAATAMRQSRP"
     gene            2676..>3131
                     /locus_tag="VG274_02945"
     CDS             2676..>3131
                     /locus_tag="VG274_02945"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWE05637.1"
                     /translation="MIALSRRDMLRAGGAIVVSFSLVRQTRGHGQNLPGDLKDAPFLD
                     SWLRIGSDGRVTVFTGKAELGQGIKTAIIQVAAEELDLLPDRIAIVTADTAQTPDEGF
                     TAGSHSMQGSATAVMNAAAQARALLIAEAARQWNLRPASLSARDGVVAAP"
BASE COUNT          571 a          992 c         1052 g          516 t
ORIGIN      
        1 cacctccccc acgcgcgaag cgcgcggggg aggaaaacca aatgccggcg cgagcggctt
       61 ggcggggttc atgcggcgga acatttgtga aagaatgccg tcgccgtcgc agaagaaggg
      121 tcacatgtcc cgctacaccg cacttgttga cggcaaagcc ggcgcttacg gcgttgtgtt
      181 tcccgaactg gacggctgcg cggcgatggg cacaaccatc gacgaggcgc ttgcaaatgc
      241 ggcagaggca cttcgcgatt gggtcgaggt cacggaggaa catggcgagc acgtgccatc
      301 gcctcaacct cttgagaaac tgcggcgccg gcgtcaagta atggaagcgc ttgcagaagg
      361 ggcagttctg gcaaccgtgc cgctggtgcg tgaaaccggc agacctgcaa aggcaaacct
      421 ctcgcttgat tcgggagttc tggccgccat cgacgaagag gcggcgcgac gcaagctgac
      481 acgttccgcc ttcatcgagt ttctcgcccc ggaactgctt ccgaaggtgg cgtgaagcga
      541 accgcctaat tcgtgcataa gccgcgctgg ctcagccagc gttccgcggc ttcgagatcg
      601 gcgggggtgt tgatgttgaa gaacgggtcg ctgtccgggc cgccttccgc agcgaaatcc
      661 gcccaggccg gcacgagcca gtcctcgacc tcgcgcatgc tgcgcgtgcc ctgggcgaaa
      721 gcggcgtcga gctggtcgcg gcacgcgacc ggccagagcg cgaagatgcg atgggcggtg
      781 gcgccgcagc gcgcgatggc gaaatcggcc gatgatgctt gaagcgcgtg cgcgaggcgc
      841 gacaccgtgt cgagcggaaa gaagggcgtg tcgcaggcga agctcgccac atgatcgaaa
      901 ccgcgccgtc cggctctccc gagcacggtg agaatgccgg cgagcggtcc ctcgcccggc
      961 gcactgtcgg caaggcgctc cagatcggga atggatcggc caatatccgg ctcgttcgcg
     1021 ttgaggagca gtgtttcgac ctgcggccgg gcgcgctcca cgacccgttc gagcagcggc
     1081 cgtccggcga gcagcgccgc gctcttgtcg ccgccgaacc tgcggctcgc gccgcctgcg
     1141 aggatcacgc cgaggatgcg attcgccgtc atgcggattg cgcgcgcttt ctctctctcc
     1201 gcgttggtgg aggatgtcgc ttgcccagca cagcttgcaa gcccggcaga cgcacccatc
     1261 cttgacttgg accatgattc agtccaaata tttgaaccaa agactggtcc agaacgatgc
     1321 ggatatcggt aagcgacgcc aaggggcaac tgacggaact ggtccgccgg gcggaacagg
     1381 gcgacgatat cgttctaacg cggcacggcc aggcggtcgc gcgagtggtg ccaatcagaa
     1441 ccgttccaga ccgggccgcg cgtcgaaagc tgcttgaagc ggcgcaggcg tcgggcgcgc
     1501 gcaaggcgcg ccgcggcccg acagcggcgc ggagccagga ttttctctat gacaaaaacg
     1561 gtcttcccaa atgatcgcag tcgatacgtc tgcgctgatg gcgatcatcc tcggcgaagg
     1621 cgcgggtaac gcctgcatgg acgctctcga aacggaaacg gatgtgctga tttccgcggg
     1681 aacagcggca gaatcgctca tcgtggccgc gcggcgccgc gtcgcggacg agatgggcag
     1741 tctgattgag gggctcggca tcgaaatcgt gccggtaacg atgtcctcgg ctttgcgcat
     1801 cgcggacgcc tatgcgcgat ggggcaaggg cgcgcatccc gccgccctga atttcggcga
     1861 ctgtttcgcc tatgcgctgg cgaaggaaca ttcgtgccgc cttctgtatg ttggagacga
     1921 cttcgcgcgc acggatgttg accgggtttt gtagcgccat cggcatgcaa agctcggacg
     1981 ccatgatcgg gccgggcgcg cgccgcggcc cgttcgagcg gcggccgtcc ggcaagcagc
     2041 gccgcacttt tgtcgccgcc gaacctgcgg ctcgcgccgc ctgcgaaaac gactccggga
     2101 atgtgccggg acgggggcgc cataggattg atccgccatt ctgaaaccgg ccggggaacc
     2161 atcactctgg cttccgcttc cttacgggtg caagccggat gagcctgcgt catgaagctg
     2221 cgcgtgaacg gaacaatgca tgacgttgcc gccgatccgt cgacgccgct gctctatgtg
     2281 ctccgcaacg atctcggcct gaacggcgcg aaattcggct gcggtctcgg gcagtgcggc
     2341 gcctgcacgg tgctggccgg caaggacgcg atattctcct gcatcacccc cattgggtcg
     2401 ctcgcaaacc gtgacatccg cacgatcgaa ggcctcggaa caatggaaca tcccggtccg
     2461 ctccagcgtg cgttcatcga cgaacaggcc gcgcaatgcg gctattgcac agccggcatc
     2521 gtgatgcgcg cccaggcgct cctggaaacg aacgcgtccc ctacagaaga ggaaatccgc
     2581 cgccacatgc ggcccaacct gtgccggtgc ggcacccaat tgcgcgtcat ccgtgcgatc
     2641 cgccgggctg cgacagcgat gcggcagtct cgcccatgat cgccctgtcg cgacgcgaca
     2701 tgttgcgcgc gggaggcgcg attgtcgttt ctttctcgct tgtccggcag acaagaggac
     2761 acggacaaaa tcttcccggc gatctgaagg acgcgccctt cctcgacagc tggctccgga
     2821 tcggcagtga cggccgagtc accgtcttca ccggaaaggc agagctgggg caggggatca
     2881 agacggcgat cattcaggtt gccgccgagg agctcgacct gttgccggat cgcattgcga
     2941 tcgttacggc ggacacggcg cagactcccg acgaaggttt taccgcgggc agccattcga
     3001 tgcagggcag cgcaaccgcg gtgatgaacg cggccgcgca ggcgcgtgcg cttctcattg
     3061 cggaagcggc ccggcaatgg aacctgcggc cggcaagcct gtcggcgcgc gacggcgtcg
     3121 tcgcagcgcc c
//
LOCUS       DASZMC010000148         3887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5008066,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000148 DASZMC010000000
VERSION     DASZMC010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5008066"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2643)
                     /locus_tag="VG274_02950"
     CDS             complement(<1..2643)
                     /locus_tag="VG274_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HWE05638.1"
                     /translation="MSRASMIRRTPLAPAILLALASLVAIALLNSAAAVSRSVDFGGA
                     SPVLDLTWALSPYHAANGVESDDSRWYMMTASNQSVRPATRILLADQPFDTSLRILPQ
                     RGRAAIRQVATSDADVIVENAHAYGRYAFRVTIPPATTAELAVRLSNADAQPRMSAWV
                     EPFLAAHNRQLAVLFAAVAGLIAAALAIMTGLAVMTGHAAPRWAAAVLLGVLLVGLAA
                     AGLFDAVGMGGVGGPYGVTAMLSGMTLAAGVRLADTIAPTQSVFGRTVVRWVLVGLAV
                     LSAFALVGVPGAMLITDFGVVAGTALIAAYLVHEGLSGAQAARVAAPSAVVFALVTTA
                     AAVSSLGGFESDPGAPGVIGGFAATGAVLLALAIAAGEGIAILPARRSTIPPLPQKNV
                     SSVADESTQALAAIGASYQGVFELDLKRDSVKLSQEAAALLGLTKGAEQLSHADWLAR
                     MHPEDRDVYRDAMQRFRAEEGLAFRVEFRARNELGHYCWLELRASALGDGAGVERFLG
                     LVADVTTRKETDVSEGVRGERDALTGLRNRNGLIEQLEELAGDISAITLALLDIDRFK
                     SVHASLGDAGGDDILVSIARRLAALGRGKAQLFRVGGDSFALLFTRPNVNALAIGERI
                     VHALREPHVWQGRSAFAPASVGLALGGDAANPFDLIKNAELGLLKAKREGGACARLYG
                     RELENSGPPDGMELEAELRRSLAQGDLELFYQPIMRLTDGTVAGFEALLRWNHPERGQ
                     LAPSDFIAHAEQTGLIIELGSFALSRAAGDLGQWQKDLPLEPPLFVSVNVSRRQLREH
                     EFERFVVRLITSAGVKPGTLKLELTESVVSSGDGIEAVLCRLRGCGVSIAIDDFGTGL
                     STLSQLQNVPFDTVKIDKGFLPRQT"
     gene            complement(2640..3248)
                     /locus_tag="VG274_02955"
     CDS             complement(2640..3248)
                     /locus_tag="VG274_02955"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWE05639.1"
                     /translation="MNNSRMGFMRGLMFPGGQQPVIKGDDVYLRYPLVADFSPWTKLR
                     ADSRSFLAPWEPAWATDELSKGAFRRRLKRYQREAREDSAYAFFVFRGADNCLLGGCT
                     LSNVRRGVTQCCALGYWVGERFARQGYMSDAVGALIPFIFRTLGLHRIEAACLPSNEP
                     SRNLLAKAGFREEGLARRYLQINGEWQDHVLFALLSDEEQPA"
     gene            complement(3245..>3887)
                     /locus_tag="VG274_02960"
     CDS             complement(3245..>3887)
                     /locus_tag="VG274_02960"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWE05640.1"
                     /translation="TEIRASEELEQVHVVYAFPGIASGDDELFAAQVYVTALGGGMSS
                     RLFQEIREKRGLCYSIYAFANSFVDGGMIGVYAGTGESEAGEISALVAEQMSALAFTA
                     TAEEIARAKAQLRASLLMGLERPSARAEQIAAHLLAYGRVVPLAEIRAKLEAVDVGEV
                     RRFGERLLTTARPTLAAIGPVGKLERYDSFARRFGAAISRPGEAAARPAHAAE"
BASE COUNT          785 a         1256 c         1191 g          655 t
ORIGIN      
        1 cgtttggcgc ggcagaaagc ccttgtcgat cttgaccgta tcgaacggca cattctgaag
       61 ctggctgagc gtcgacaggc ccgtgccgaa atcgtcgatc gcgatgctta ctccgcagcc
      121 ccgtagcctg cacagcaccg cctcgatgcc gtcgcccgag ctgacaacgc tttccgtcaa
      181 ctccagcttc agggtgccgg gcttcacccc ggcactcgtg atcaggcgca ccacgaaacg
      241 ttcgaattcg tgttccctga gctgccggcg cgaaacattc acgctcacga acaggggcgg
      301 ctccaggggc aaatccttct gccactgacc aagatcgcct gccgcgcgcg acagcgcaaa
      361 actccccagt tcgatgatga gtccggtttg ctcggcgtgg gcgatgaaat cggacggcgc
      421 gagttgtcct ctttcggggt ggttccagcg caaaagcgcc tcgaagccag cgacagtgcc
      481 gtcggtcagg cgcataatgg gctgatagaa aagctcgaga tctccctgcg ccagcgaccg
      541 gcgtagctcg gcttcaagtt ccatcccgtc gggcggtcca gaattttcca gttcgcggcc
      601 gtacaggcgg gcgcacgccc caccctcgcg tttggctttc agaagtccca gctcggcgtt
      661 cttgatcaga tcgaaaggat tcgcggcgtc gccgccaagc gccagaccca cgcttgcggg
      721 tgcgaatgcg cttcggccct gccagacatg cggctcgcgc aaggcatgta cgatacgctc
      781 acctatggcg agtgcgttga cgtttggacg cgtgaagagc aacgcaaagc tgtcgccacc
      841 gacgcgaaac agctgtgcct tgccgcggcc gagagcagcg aggcgcctgg cgatgctcac
      901 gagtatgtcg tctcctcccg catcgccgag actcgcatgg acggatttga agcgatcgat
      961 atccagcagc gcaagagtga tcgcagagat atctccagcg agctcttcca gttgctcgat
     1021 cagtccgttg cggttgcgca ggcccgtcag cgcgtcgcgc tcgccgcgca cgccctcgga
     1081 gacgtctgtt tccttgcggg ttgttacgtc tgcaacgagg ccgaggaagc gctcgactcc
     1141 ggctccatct cccagcgcgc tggcgcgaag ctcaagccag caataatggc cgagttcgtt
     1201 tcgggcacga aattcgacgc gaaacgcaag accctcctcg gcacgaaaac gctgcatcgc
     1261 atcgcggtag acgtctcgat cctcgggatg catgcgcgcg agccagtcgg cgtgtgaaag
     1321 ctgctcggcg cctttggtca gaccaagcag cgctgccgct tcctgcgaaa gcttgacgct
     1381 gtcccgtttc agatcgagtt cgaaaacgcc ttgataggaa gcgccaattg cggcaagcgc
     1441 ctgcgtggat tcgtccgcaa ccgatgaaac attcttttga ggcaatggcg ggatggtcga
     1501 gcgtcgtgcg ggaaggatgg cgataccctc gcccgccgcg attgcgagcg cgagcaggac
     1561 ggcccctgtt gccgcgaaac cgccaatcac gccgggagcc cctggatcgg actcaaagcc
     1621 gccaagtgac gacaccgcgg cggccgtcgt tacgagcgcg aagaccacag cgctcggcgc
     1681 agccacacgc gcggcctgtg cgccagagag tccctcgtgc acgagatagg ccgcaatgag
     1741 ggccgtgccg gcaaccactc caaagtccgt aatcagcatt gcgcccggca cgcctacgag
     1801 cgcgaaggcg gaaaggacgg ccagaccgac caggacccag cgcacaactg ttcggccgaa
     1861 cacactttgc gtcggtgcga tcgtgtccgc gaggcgaact cctgcggcta gcgtcatgcc
     1921 ggaaagcatg gccgtcacgc cgtaaggccc gccgacgccg cccatcccca cggcatcgaa
     1981 caatccggct gcggccagtc cgaccagcag cacccccaac agcacggccg ccgcccagcg
     2041 tggcgcggca tgacccgtca tcactgcaag cccggtcatg atggccagag ctgctgcaat
     2101 caggccggcg acggcagcaa agagtacggc aagctgccga ttgtgcgccg cgaggaaagg
     2161 ctcgacccac gcgctcatcc ggggctgggc gtccgcgttc gaaagtctga ccgcgagctc
     2221 cgcggtcgtg gcgggcggta tcgtgacgcg aaacgcgtaa cggccatatg cgtgggcatt
     2281 ctcgacgatc acgtcggcat ccgatgtggc aacttgcctg atcgcggcgc ggccgcgctg
     2341 cggcaaaatg cgaaggctgg tgtcgaatgg ctgatcggca agaagaatgc gcgtggcggg
     2401 acgcacggac tggttggagg ccgtcatcat gtaccagcgc gaatcatcgc tctcgacgcc
     2461 gttggccgca tgatagggcg acagcgccca tgtgagatcg agcactggcg acgcgccgcc
     2521 gaaatctacg ctgcgggaaa cagcggcggc agagttcaaa agtgcgatcg cgaccaagct
     2581 cgcaagagcc agcagaattg ccggagcaag aggggtgcgg cggatcatcg atgcgcgact
     2641 caagcgggtt gttcttcgtc ggacaacaac gcaaagagga cgtgatcctg ccactcgccg
     2701 ttgatctgca aatagcggcg tgcgagaccc tcctcgcgaa atccggcctt ggcaagaaga
     2761 tttcgcgacg gttcgttcga aggcaggcac gcggcttcga tcctgtgcaa accgagcgtg
     2821 cgaaaaatga agggaatcag cgcgccgacg gcatccgaca tgtagccctg tcgcgcgaag
     2881 cgctcgccga cccaatagcc gagagcgcaa cattgggtga ctcctcggcg tacgtttgaa
     2941 agggtgcatc cgccaagcag acaattgtcc gctccgcgaa agacgaagaa agcataggcg
     3001 gaatcctcgc gtgcttcgcg ctgatagcgt ttgaggcggc ggcggaacgc ccccttgctc
     3061 agttcgtcgg tcgcccaagc cggctcccac ggcgcaagaa agctgcggct atcggcccga
     3121 agcttcgtcc atgggctaaa atccgcgacg aggggatagc gaagatagac atcgtcgccc
     3181 ttgatcaccg gctgctggcc gccgggaaac atcagtcctc gcatgaaacc catgcgactg
     3241 ttgttcattc cgcagcgtgc gccgggcgcg cggccgcctc tcccggccgg gatattgccg
     3301 cgccgaaccg gcgagcgaaa ctgtcgtaac gctcaagctt tccgacaggt ccgatagctg
     3361 caagcgtggg ccgtgccgtc gtcagcagcc tttcaccgaa acgccgcact tcccccacat
     3421 cgaccgcttc gagcttggct ctgatctctg ccaacggaac gacccggcca taagcgagaa
     3481 ggtgcgcagc gatctgttcg gcgcgcgcgg aaggtcgctc aagacccatg agcaaactcg
     3541 cgcggagttg agccttcgcg cgcgcgattt cctcggcggt cgctgtgaag gcgagcgcag
     3601 acatctgctc cgctaccaga gcagaaatct cgcctgcttc gctttcgccc gtgcccgcat
     3661 acaccccgat cattcccccg tcgacgaaag aatttgcgaa ggcatagatg gaataacaga
     3721 gcccacgctt ctccctgatt tcctgaaaca ggcgcgagga catgccgccg ccaagcgccg
     3781 tcacataaac ctgcgcggca aagagctcgt cgtctccgct ggctattccc ggaaacgcat
     3841 agacgacatg cacctgttcc aattcttccg atgcgcgaat ttcggtg
//
LOCUS       DASZMC010000149         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5008253,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000149 DASZMC010000000
VERSION     DASZMC010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5008253"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VG274_02965"
     CDS             complement(<1..944)
                     /locus_tag="VG274_02965"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWE05641.1"
                     /translation="MILVTGAAGFIGFHVAQALLARGQPVLGVDNLNPYYDVTLKAAR
                     LSRLEANPDFVFRKTDISDRAAMLELPRVFPNVSGIVHLAAQAGVRHSLTDPYVYVQA
                     NVMGQVVMLELARTCPKLVHFVYASSSSVYGGNDKLPFAVGDPVTQPNSLYAATKRAD
                     ELIGYTYAHLYHVPATGLRFFTVYGPWGRPDMAAFIFARAILAGEPIRVFNHGEMWRD
                     FTYIEDVVSGVLRALDRPPAGAPPHALYNLGNHRSEKLTDFIAVLESTIGKKAEILLE
                     PMQLGDVPATYADIESSRRDLGFEPTTPIAEGIPRFIR"
     gene            1100..>2552
                     /gene="asnB"
                     /locus_tag="VG274_02970"
     CDS             1100..>2552
                     /gene="asnB"
                     /locus_tag="VG274_02970"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWE05642.1"
                     /translation="MCGIAGEARFDGGVAAAPLEAMSQGIRHRGPDDQGLWIDRDRNC
                     GFSFRRLSIIDLSPLGHQPMVDPETGNVIAYNGEIYNFQKLRRECEQAGARFRTRSDT
                     EVILALYRRFGPDCVKQLRGMFALAIWDARKSELFLARDRFGKKPLNYALTKNGLAFC
                     SEIGPLSQHPAVGREHDADALELYLQLLVVPAPWTIYRNIRKLPPAHWAVFSRAGLRI
                     EKYWDLDYTKKIRLTDAEALEGFEERFTEAVRLRMIADVPLGALLSGGVDSSAVVAQM
                     AKCSTTPVRTFSVGFEEESFNELPYAAQGARICGADHHEQIVTSDVELLLPLIVRHYG
                     EPFADPSAIPSFLVCEAARENVTVVLNGDGGDELLGGYPRYRLSNTTLLASRLLGKVV
                     PPGLLLPAAVNFSGGADFFDRAISRLTREFIHPEFGSLMNYRGNWHDNARPDLLLGGG
                     NSGLLPDWRRRWLDAACRHAGNPVDRMLYFDNHT"
BASE COUNT          477 a          766 c          840 g          469 t
ORIGIN      
        1 cagcggatga agcgggggat gccttcggcg atgggcgtcg tgggctcgaa gccgaggtcg
       61 cggcggcttg attcgatgtc ggcataggtc gcaggcacgt cccctagctg catcggctcg
      121 agcagaatct ccgctttctt gccaatcgtg gattccagta ccgcaatgaa gtcggtcagt
      181 ttttccgaac ggtgattgcc gagattgtag agcgcgtgcg gcggcgcgcc cgcgggcggc
      241 cggtcgagcg cgcgcaggac gccgctcacg acgtcctcga tataggtgaa gtcgcgccac
      301 atctcgccgt gattgaagac gcggatcggc tcgccggcga ggatcgcacg cgcgaagatg
      361 aaagccgcca tgtcagggcg tccccaggga ccgtagacgg tgaagaatcg cagcccggtc
      421 gcagggacat gatagaggtg ggcataggtg tagccaatca gctcgtcggc gcgcttcgtc
      481 gcggcataga gggaattcgg ctgcgtgacc ggatcgccaa ccgcaaacgg cagcttgtcg
      541 ttgccgccgt atacggatga ggaactggca tagacgaaat ggacgagctt cggacaggtg
      601 cgcgccagct cgagcatgac cacctgtccc atgacgttgg cctgcacgta gacgtagggg
      661 tccgtcagcg aatggcgcac gcccgcctgc gccgcgagat ggacaatgcc cgagacgttg
      721 gggaacacgc gcggcaattc cagcatcgct gcgcggtcgg agatgtcggt tttgcggaag
      781 acgaaatccg gattggcctc gaggcgggag aggcgcgccg ccttgagcgt cacgtcgtaa
      841 tagggattga gattgtcgac gccgagcacc ggctggccgc gggcgaggag ggcctgcgcc
      901 acatggaagc cgatgaaccc tgcggctccc gtcaccagaa tcatggacac ctcgcgaatc
      961 gctccacccg tttatgccac gaacggagga gcaggcttgc acctttgcca aggctggaac
     1021 gtagagccat tatggttaca ttataaagtg gggaagaagg ggcggcatcg aggcgccgca
     1081 aaggagcagg cggggttaaa tgtgcggcat tgccggcgag gccaggtttg acgggggtgt
     1141 ggccgcggcc ccgctcgagg cgatgtcgca gggcatccga caccgcggcc ccgacgatca
     1201 aggcctctgg atcgaccggg accgcaattg tggtttttcc ttccgcaggc tgagcatcat
     1261 tgatctgagc ccgctgggcc accagcccat ggtggatccg gagaccggaa acgtcatcgc
     1321 ctacaatggc gagatctaca atttccagaa gctgcggcgc gaatgcgaac aggcgggagc
     1381 gcgattccgc acccggagcg acaccgaagt cattcttgcg ctctatcggc gtttcggtcc
     1441 ggattgcgtg aagcagttgc gcggcatgtt tgcgctggcg atctgggatg cacgcaaatc
     1501 cgaattgttt ctcgcccgcg accgcttcgg caagaagccg ctgaactatg cgctgacaaa
     1561 aaacggcctg gcgttctgct ctgaaatcgg cccactgtcg cagcacccgg ccgtgggacg
     1621 cgagcacgac gcggatgcgc tggagctgta ccttcagctt ctcgtcgtgc cggcgccctg
     1681 gacgatctac cgcaacatcc gcaagctccc gcccgcgcat tgggcagttt tttcccgggc
     1741 cggccttcgc atcgagaagt attgggatct cgattacacg aagaagatcc gtctgaccga
     1801 cgcggaagcg ctggaagggt tcgaggaacg attcaccgag gcggtgcggc tgcgcatgat
     1861 cgccgatgtg ccgctcggcg cgctgctcag cggcggcgtc gattcgagcg ccgtcgtcgc
     1921 ccagatggcg aaatgttcga ccacgccggt caggacattc agcgttggtt tcgaggaaga
     1981 gagtttcaac gagcttccct acgcggcgca aggcgcgcgc atttgcggcg ccgaccatca
     2041 cgaacagatc gtcacgagcg atgtggagct gctgctgccc cttatcgttc gccactatgg
     2101 ggaaccgttt gccgatccgt ccgccatccc gtctttcctc gtctgcgagg cggcgcgtga
     2161 aaacgtcacc gttgtgctca atggcgatgg cggagacgag ctgctcggcg gctatccgcg
     2221 ctaccggctc tccaacacga cgcttcttgc gagtcgactg ctgggaaagg ttgttccgcc
     2281 cggattgctc ttgcccgcgg cggtgaattt ctccggggga gccgatttct tcgaccgcgc
     2341 aatcagccgt ctgacgcgcg aattcatcca tcccgaattc ggttcgctga tgaactatcg
     2401 cggcaactgg cacgacaatg cgcgcccgga ccttctcctc ggcggcggca attccggatt
     2461 gctgcccgat tggcgccgca ggtggctcga cgcggcgtgc aggcacgcgg gcaatcccgt
     2521 cgaccggatg ctctatttcg acaatcacac ct
//
LOCUS       DASZMC010000150         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1719428,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000150 DASZMC010000000
VERSION     DASZMC010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719428"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VG274_02975"
     CDS             complement(<1..358)
                     /locus_tag="VG274_02975"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF014870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWE05643.1"
                     /translation="MSLVSKTIGSPVGRLKLVASDKGLVAILWHNDRPNRVPLEDVVE
                     DHQHPVLVRTEKQLSEYFAGARTSFDLPLDMRGTVFQKNVWEALLAIPFGETRTYAGL
                     ANRLGNPRATRAVGAAN"
     gene            complement(418..>1541)
                     /locus_tag="VG274_02980"
     CDS             complement(418..>1541)
                     /locus_tag="VG274_02980"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HWE05644.1"
                     /translation="FLEHLGASPISVAQTRRVLFAKQLIHETRMPMTEVALASGFSSL
                     RRFNETFQHLFHRPPSALRRRSSAVFEEKDVVLRLRYRPPYDWENMLAFLRARAIPGV
                     EIVEATRYLRTVNIGRALGSIEVTHQPAHNSLSVRIRFPDVRSLPAIVARVRRLFDLG
                     ANIQTIDEHLSGDPILAPLIAERPGLRVPGGWDSFEIAIRAILNQQISVVAARRLAGQ
                     LVALHGRTIPNSFRIHPELTHAFPSARQLVAASSIGLAIPSARLTALRAVAEAAAADP
                     NLFRAFGSVEETVARLRTIRGIGEWTSQYIALRAIREMDAFPATDLGLLRESRSFQGE
                     RSTAAALLTRAEAWRPWRAYAAQHLWTAGAHTASARISN"
BASE COUNT          291 a          423 c          559 g          268 t
ORIGIN      
        1 cgttagccgc gcctacagcc cgcgtggcgc gtggattgcc gagacggttg gcgagaccgg
       61 cgtaggtgcg agtctcaccg aaaggtatcg caagcagcgc ctcccagaca tttttctgga
      121 acacggtacc gcgcatgtcg agcggaaggt cgaagctcgt tcgggcgccc gcgaagtatt
      181 cgctcagctg cttctccgtg cgaacgagga ctggatgctg atggtcttcg accacgtctt
      241 cgagaggcac gcggttgggg cgatcgttgt gccagagtat ggctacgagg cctttgtcgc
      301 tggcgaccag cttgagcctg cctaccggcg acccgatggt tttcgatacg agggacatag
      361 cttcctttct gcgctctctt tctgcgagag cggcgcaaag atcgaatgca tctgctgtca
      421 gttcgaaatc cgggcggatg ccgtatgtgc accggcagtc cacagatgtt gtgccgcgta
      481 ggcgcgccac ggccgccacg cctcggcgcg ggtgagcagc gctgctgcgg tagatcgctc
      541 gccctggaag gagcgcgatt cgcgcagcag gccgagatcg gtcgccggaa aggcatccat
      601 ctcgcggatg gcacgaagcg cgatgtactg tgatgtccac tcgccgatgc cgcgaatggt
      661 gcgcagacgc gcaacggtct cttcgacgct gccgaaggcg cggaagagat tgggatcggc
      721 ggccgctgcc tcggcgacgg cgcggagcgc ggtcaggcgc gctgacggaa ttgccagtcc
      781 gatggaagat gccgcgacga gctgtctggc agagggaaag gcatgggtaa gttcgggatg
      841 gatgcggaaa gagttgggga tggtccggcc atgaagcgcg acaagctgac ccgcgagacg
      901 gcgcgccgcg acgacgctga tctgttgatt caaaatcgcg cgaatggcga tctcgaagct
      961 atcccagccg ccgggcacac gcaggccggg gcgctcggcg atgagcggcg cgagaatcgg
     1021 gtcgccggag agatgctcgt cgatggtctg gatgttggcg ccgagatcga agagccggcg
     1081 cacacgcgcg acgatggcag gcagtgagcg cacatcggga aaacggatgc ggacgctgag
     1141 actattgtgc gcaggctgat gtgtgacctc gatagagccc agggcgcggc cgatgtttac
     1201 ggtgcgcagg taccgcgttg cttcgacgat ctctacgccg gggatggcgc gtgcgcgcag
     1261 gaaggcgagc atgttttccc agtcgtacgg gggcctgtag cgaagacgca gaacgacgtc
     1321 tttttcttcg aagacagcgg acgatcgcct gcgcagcgca ctgggcgggc ggtgaaacag
     1381 atgctgaaag gtttcgttga accggcggag gctgctgaag cccgaggcga gcgcaacctc
     1441 ggtcatgggc atgcgggttt cgtgaatgag ttgcttggcg aacaggacgc ggcgcgtctg
     1501 cgcgaccgag atgggagagg cgccgagatg ttcaaggaag a
//
LOCUS       DASZMC010000151         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1171649,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000151 DASZMC010000000
VERSION     DASZMC010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171649"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /gene="murG"
                     /locus_tag="VG274_02985"
     CDS             <1..1130
                     /gene="murG"
                     /locus_tag="VG274_02985"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWE05645.1"
                     /translation="AVRVSTGGARCMTTIVLSAGGTGGHLFPARALAEELLRRGRSIV
                     VMTDRRGRKSDTVFEGAIVESVPSATFAGSLASRFAAPVQILCGIFVSWRKLAQIKPG
                     AVVGFGGYPSFPVMIAAWLAGFPTAIHEQNAVLGRVNRMLSMRVSAIAASFTLMRFAP
                     KGTSFIVYTGNPVRPEAAELSSAPYAEPSEDGAIRLLVFGGSQGAHALSELVPAAIAL
                     LPELLRRRLEIVQQCRPEDIEQVRAAYAARNVGAELAPFFNDLPARMAAAHLVISRSG
                     ASTISELAVIGRPAILIPYPFATDDHQTANARVFADAGAAWLVQQRDVTPERFAQRLE
                     MILGAPVELARRAGAARALARPDAASRLADLVDRIARPEAA"
     gene            1130..2557
                     /gene="murC"
                     /locus_tag="VG274_02990"
     CDS             1130..2557
                     /gene="murC"
                     /locus_tag="VG274_02990"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWE05646.1"
                     /translation="MRDAPTRVPFDIGTIHFIGIGGIGMSGIAEIMRNLGYRVQGSDA
                     VENANVKRLRALGIPVALGHDAGNLGDAVVVVYSSAVKPGNAEFEAARKRKLPLVRRA
                     EMLAEIMRLKSCVAVAGTNGKTTTTTLVAALFDAGGLDPTVVNGGVINAYGTNARLGA
                     GEWVVVEADESDGTFLRLPATIAVVTNIDPDHLDFYGTFERMRAAFQRFVENVPFYGF
                     AVLCLDHPEVQAMVGRIEDRRLITYGFNPQADVRATNVTCSQGAAHFDASILDRRTGR
                     ETLLPQLSLPMPGEHNVQNALAAIAVAAALGIDRDCIREALARFAGVTRRFSRVGEWH
                     GAAIIDDYAHNPFKIAAALKAARQAYSGPIVAIVQPHRYSRLRDTFEQFSTCLHDSDT
                     AIVAPVYAAGEQPLPGFDRDSFADSLRAHGHRHVLVIDGPDDLPPLLADLAKPDGAIV
                     FLGAGSITHWAAGLQSALSRMETRT"
     gene            2557..>3108
                     /locus_tag="VG274_02995"
     CDS             2557..>3108
                     /locus_tag="VG274_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWE05647.1"
                     /translation="MNTRLREAWPAVRGKWQERAPLKEFLWFRAGGPAEFLFRPADEE
                     DLCAFLAAKARSLPVTVIGVGSNLLVRDGGIRGAVIRLSSSFGRCTVEKDRVRAGAAA
                     LDAAVARAAADAGLGGLEFLRGIPGTIGGALRMNAGCYGREIKDVFVEAVAVDPGGRR
                     IVLKAGDISFSYRSCSAPEGLIFT"
BASE COUNT          495 a         1007 c         1005 g          601 t
ORIGIN      
        1 gagctgttcg cgtttctacg ggaggcgcgc gctgcatgac gacgatcgtg ctctccgccg
       61 gaggcacggg cggtcatctt tttcctgccc gggcgcttgc cgaggagctt ctgcgtcgcg
      121 ggcgaagcat tgtggtgatg accgacaggc gcgggcgcaa gtccgatacc gttttcgagg
      181 gcgcgatcgt cgaatcggtg ccgtcggcga cgttcgccgg atcgcttgcc tcacggttcg
      241 ccgcgcccgt gcagatcctc tgcggcatct ttgtcagctg gcggaagctc gcgcagatca
      301 agcccggcgc cgttgtcggg ttcggtggct atccgagctt tcccgtgatg attgcggcgt
      361 ggctcgccgg ctttcctacg gccatccacg agcagaacgc agtgcttggg cgcgtcaacc
      421 gaatgttgtc catgcgtgtg agcgccattg ccgcatcgtt tacactcatg cgattcgcgc
      481 cgaagggcac atcgttcatc gtgtataccg gaaatcccgt ccggccggag gctgcggagc
      541 tttcgtctgc gccttacgcg gagccgagcg aagatggcgc gattcggctg ctcgtttttg
      601 gcggcagcca gggagcgcat gcgctcagcg aactggttcc cgcggcgatc gcgctcctgc
      661 cggaattgct gcggcggcgg ctggaaattg ttcagcaatg tcgtcccgag gacatcgaac
      721 aggtccgcgc ggcctatgcg gccaggaatg tcggggccga actggcgccg ttcttcaacg
      781 atcttcctgc gcggatggcg gcggcacacc tcgtgatctc gcgcagcggc gcctccacga
      841 tcagcgaact tgccgtgatt ggccgccctg caatcctgat cccatacccc tttgctaccg
      901 acgatcatca gacggcgaat gcgagggttt tcgccgatgc gggagccgcc tggcttgttc
      961 agcagcgcga tgtcacgccc gagcgtttcg cacagcggct cgaaatgatt ctcggcgccc
     1021 cggtggagct ggcgcggcgg gccggggccg cacgtgcact ggcgcgtccc gacgccgcct
     1081 cgcgccttgc cgaccttgtc gaccggatcg cgcgtccgga ggcggcatga tgcgtgatgc
     1141 tccaacccgc gtgcctttcg acatcggcac gatccatttc atcggcatcg gtggcatcgg
     1201 catgagcggc attgccgaga tcatgcgcaa tctcggctat cgcgttcagg gctccgatgc
     1261 ggtcgaaaac gcaaatgtga agcgattgcg cgccctcggc attcccgttg cgctcggaca
     1321 tgatgccgga aatctcggcg acgccgtggt cgtggtctat tcctcggcgg tcaaacccgg
     1381 caatgccgag ttcgaagcgg cccgcaaacg caagcttccg ctggtccggc gcgccgaaat
     1441 gctggccgag atcatgcgac tgaagagctg tgtcgcggtc gccggcacca atggaaagac
     1501 cacaaccaca acacttgtcg ccgccttgtt cgacgcgggc ggtcttgatc ctaccgtcgt
     1561 caacgggggc gtcatcaacg cctacggaac caatgcgcgc ctgggcgctg gcgaatgggt
     1621 ggtggtggaa gccgacgaaa gcgacggcac ctttctcagg ctgccggcga cgattgcggt
     1681 cgtcaccaac atcgatcccg atcatcttga tttctacggc actttcgagc gcatgcgcgc
     1741 ggcgtttcag cgcttcgtcg agaatgtacc cttctacgga tttgcggtgc tctgcctcga
     1801 tcatcccgaa gtgcaggcga tggtcggccg gatcgaggac cggcggctta tcacgtacgg
     1861 attcaatccc caggccgatg tacgcgccac caacgtgacc tgctcgcaag gcgccgcgca
     1921 tttcgatgcg agcattctcg accggcgaac gggacgcgag acgctcctgc cgcaactgag
     1981 cctgcccatg cccggcgaac acaatgtgca gaacgcgctc gcggccatcg cagtcgccgc
     2041 ggcgctcggc atcgaccgcg actgcatccg cgaagcgctc gcgcggttcg cgggcgtcac
     2101 gcgccgtttt tcgcgcgtcg gcgaatggca cggcgcagcg attatcgacg actatgcgca
     2161 caatccgttc aagatcgccg ccgccctgaa ggctgcgcgg caagcctatt ccggccccat
     2221 cgtggccatc gtgcagccgc accgctattc gcgcctgcgc gacacgttcg agcaattctc
     2281 cacctgcctg cacgattcgg acacggcgat cgtggcgccc gtctacgccg ccggtgaaca
     2341 accgcttccg ggattcgatc gcgactcttt cgcagactcg ttgcgcgccc atggacatcg
     2401 ccacgttctt gtcatcgacg ggcctgacga tctgccgccg cttctggccg atcttgccaa
     2461 accggacggg gcgatcgttt ttcttggtgc gggatcgatc acccactggg cggcagggct
     2521 tcaatcagcg ctgagccgaa tggagacgcg aacgtgatga atacgcgact ccgcgaagcg
     2581 tggcctgcgg tgcgcggcaa atggcaggaa cgcgcgccgc tcaaggaatt tctctggttt
     2641 cgcgccggcg gccccgcgga attcctcttc cggccggcgg acgaagagga tctttgcgcc
     2701 tttctcgccg cgaaggcccg atcgctgcct gtcacggtga tcggcgtggg ttccaatctc
     2761 ctggtgcgcg atggcggcat cagaggcgcc gtcatccggc tctcttcgag ctttggccgt
     2821 tgcacggtcg agaaggatcg tgtgcgcgcg ggtgctgcgg ctctcgacgc cgctgtcgcc
     2881 cgcgcggccg ccgatgccgg cctgggcggt ctcgaatttc tgcgcgggat tcccggaacg
     2941 atcggcggcg cgttgcgcat gaacgcagga tgctacggcc gggagatcaa ggatgtgttc
     3001 gtcgaagcgg tggccgtcga tccaggcggc cggcggatcg tgctgaaggc gggtgacatt
     3061 tccttttcct acagaagctg cagcgcaccg gaaggcctca tcttcacc
//
LOCUS       DASZMC010000152         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3084990,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000152 DASZMC010000000
VERSION     DASZMC010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3084990"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1572
                     /gene="gcvPA"
                     /locus_tag="VG274_03000"
     CDS             226..1572
                     /gene="gcvPA"
                     /locus_tag="VG274_03000"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWE05648.1"
                     /translation="MRYLPLTQYDRRAMLDRIGAPDIDALFRDVPKEAAVGREAFALA
                     DHQGELEVERALSRLASKNVSAGSVPFFCGAGAYRHHVPAAVDHLIQRSEFLTSYTPY
                     QPEIAQGTLQYLYEFQTQVAMLTGMEVANASLYDGSTATSEAVLMAQRLTRRPKAILS
                     GGLHPHYAETVETLGALSGTVVRAPLTPGRAEDLVSLIDAETACVVVQSPDVFGLIRD
                     LRPVAEAAHAKGALLIAVVTEIVSLGLLESPGAMGADIVAAEGQSIGNGLSFGGPYVG
                     LFATREKFVRQMPGRLAGETVDAEGRRGFVLTLSTREQHIRREKATSNICTNSGLCCL
                     AFTIHLALLGEEGFVRLARANHARAAALAERLAALRGVSLVTPSFFNEFTLKLPNAAA
                     PVIDRLAERNILGGVPASRLLPHEPVARDLLIVAATETTTDEDAKAFADALGECLS"
     gene            1569..3134
                     /gene="gcvPB"
                     /locus_tag="VG274_03005"
     CDS             1569..3134
                     /gene="gcvPB"
                     /locus_tag="VG274_03005"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWE05649.1"
                     /translation="MTMNAIGRPTSTGVATPLSPPTISGDRGLDHEEPLLFELGRAGV
                     SGVDIPPTEISGSRLGDLRRKGAIGLPGLSEPEIVRHYVRLSRMNYAIDAGLYPLGSC
                     TMKHNPRLNEKMARLPGFGDLHPLQPPQTTQGALGLIAELMRWLTALTGMPAVAMSPK
                     AGAHGELCGLLAIRAAVAARGEKRARVLVPSSAHGTNPATAALAGFSVDEVPAKDDGH
                     VDADALEKMLGADVAAIMLTNPNTCGLFEKRVKEIADAVHKAGAYFYCDGANFNAIAG
                     RVRPGDLGIDAMHINLHKTFSTPHGGGGPGSGPVVLSAALAPFAPLPLLVQDENGFRL
                     IEDREELPAGARPFGRMCAFHGQMGMFVRALAYMLSHGADGVRQASEDAVLAANYILA
                     SLKDTLSAPFEGPCMHEALFDDSFLEGTGVSTLDFAKAMIDEGYHPMTMYFPLVVHGA
                     MLIEPTESESKESLDRFIHVMRALAEEAKAGRLERFAGAPHLAPRRRLDETRAARKPV
                     LRWTQNPLPQAAE"
     gene            complement(3144..3818)
                     /locus_tag="VG274_03010"
     CDS             complement(3144..3818)
                     /locus_tag="VG274_03010"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWE05650.1"
                     /translation="MSAKFWVALGIEAAIAVVLFGAAGTLSWPPAWAFVGVSLVVAFL
                     ITRMVARHDPAILKNSLRWPILRAELPWDGIFKVVFTVLFPGWLALMGLDAVRFDWSW
                     MPGWLQIAGGIGVAAALSSIYRVFEENTSLWRSLRQRTERGRQIISTGPYAVVRHPLY
                     AAGVILFVSTALLLGSWLGLAGVLVLASAIVFLTAMEDRELRLKLEGYADYSRRVRYR
                     LLPGVW"
BASE COUNT          733 a         1266 c         1266 g          686 t
ORIGIN      
        1 gaagcccagt acacggaatt cgtcaaacta ctctgatcaa ggtgatgcgt gcagcgaaaa
       61 agatttagcg gacaggacgc agaggcagcg caggggacgc agaaaaaagt ctgcgcgccg
      121 aaggcgcgca attccttccc ccgcattctc tgcgaatcct ctgcgtcctc tgcgctaaaa
      181 tcttttactc actcatcgtc aaggttaggc accaggtaaa gagcgatgcg atatctacca
      241 ctcacgcaat atgaccggcg tgcgatgctg gacaggatcg gcgctccaga tatcgacgcg
      301 ctgttccgcg acgtgccgaa ggaagcggcg gttgggcgcg aggcattcgc gcttgccgat
      361 caccagggcg agcttgaagt cgagcgtgcg ctttcgcgcc tcgcgtccaa aaacgtctcc
      421 gccgggtccg tgccgttctt ctgcggcgcg ggcgcctatc gccatcatgt ccccgcggcc
      481 gtcgatcacc ttatccagcg ctcggaattc ctcacctcct acacgcccta ccagccggag
      541 atcgcgcaag gcacgctgca gtacctctac gaattccaga cccaggttgc gatgctgacc
      601 ggtatggagg tggcaaacgc gtcgctctat gacggctcga cggcgacgtc cgaagcggta
      661 ttgatggcgc agcgcctcac gcggcggcca aaggcgatcc tttcgggcgg gttgcatccc
      721 cattacgccg aaaccgtcga aacattgggc gcgctgtccg gcacagtcgt gcgcgcgccg
      781 ctgactcccg gtcgggcgga agacctcgtc tctctgatcg acgcggagac ggcctgcgtg
      841 gtcgtgcaga gtcccgatgt attcggcctg attcgcgatc tgcgccccgt cgcggaagcg
      901 gcgcatgcca aaggcgctct gttgatcgcg gtcgtgacgg aaatcgtttc gctcggcctg
      961 ctcgaatcgc caggcgcgat gggcgcggac atcgtggccg cggagggcca gtcgatcggc
     1021 aacgggctga gtttcggcgg accctatgtc ggcctttttg caacgcgcga gaaattcgtc
     1081 cgccagatgc cggggcggct ggccggtgaa acggtcgatg cagaaggccg gcgtggtttc
     1141 gtgcttacgc tgtcgacgcg cgagcagcat atccgccgcg aaaaggcgac gagcaacatc
     1201 tgcacgaatt cgggcctgtg ctgtctcgct ttcacgattc atctggcact gctgggcgag
     1261 gaggggtttg tgcgcctggc gcgcgccaat cacgcgcgcg cggccgcgct ggccgagagg
     1321 ctggccgcgc ttcgcggcgt ttcgctggtc acgccatcct tcttcaacga attcaccctg
     1381 aagcttccga acgccgcagc gccggtcatc gatcgtctcg ccgagcgcaa cattctcggc
     1441 ggcgttccgg catcgcggct tctgccgcac gagccggttg cgcgcgatct gctgatcgtc
     1501 gcggcgacgg aaacaaccac cgacgaagat gccaaagcgt ttgcggacgc gcttggggag
     1561 tgcctgtcat gacgatgaac gcaatcggac gccccacttc gaccggcgtt gccacgccac
     1621 tgtcgccgcc gacgatttcg ggagaccgcg ggctcgacca tgaggaaccg ctgctctttg
     1681 agctgggccg cgcgggcgtc agtggcgttg atattccgcc tacggagata tccggcagcc
     1741 gtctgggcga tttgcgccgc aagggggcta ttggacttcc cggcctgtcg gaaccggaaa
     1801 ttgttcgcca ctatgtgcgt ctgtcgcgca tgaattatgc gatcgacgcg ggcctctatc
     1861 cgctcggttc gtgcacgatg aagcacaatc cgcgtctcaa cgagaagatg gcgcggcttc
     1921 cggggttcgg cgaccttcac ccactgcagc cgccgcagac cacacagggc gcgctggggc
     1981 tcatcgccga gttgatgcgc tggcttacgg cgctgacggg aatgcccgca gtcgcgatga
     2041 gcccgaaagc cggcgcgcat ggcgagttgt gcggattgct cgccatccgc gcggctgtcg
     2101 cggcgcgcgg cgagaaacgc gcgcgcgttc tcgtgccctc gtccgcacac ggcaccaatc
     2161 cggcgacggc ggcgctcgcg ggattttccg tggacgaggt ccctgcgaaa gacgatggcc
     2221 atgtcgacgc cgatgcgctg gaaaagatgc tcggggccga cgtcgcggcc atcatgctga
     2281 ccaatcccaa tacctgcggc ctcttcgaga agcgggtgaa ggagatcgcc gatgcggtgc
     2341 acaaggccgg cgcctatttc tattgcgacg gcgccaattt caacgccatc gccggccgcg
     2401 tgcggccggg tgatctcggc atcgacgcga tgcacatcaa cctgcacaag acgttttcca
     2461 ctccgcacgg cggcggcggc cccggctcgg gtccggtggt gctttcagcg gcgctcgcgc
     2521 cgttcgcgcc tttgccgctg ctcgtgcagg acgagaacgg atttcgactg atcgaagatc
     2581 gcgaggaatt gccggccggg gcgcggccct tcggccgcat gtgcgccttt cacggacaga
     2641 tgggcatgtt cgtgcgcgcg cttgcctaca tgctgagcca cggggccgac ggcgtgcgcc
     2701 aggcgtcgga agatgccgtg ctggcggcga actacattct cgcttcgctc aaggatacgc
     2761 tgagtgcgcc cttcgaaggg ccgtgcatgc acgaggcttt gttcgacgat tcattcctgg
     2821 aaggaacggg cgtatccaca ctcgatttcg ccaaggcgat gatcgacgag ggctatcatc
     2881 cgatgacgat gtattttccg ctggtggtgc acggagcgat gctgatcgaa cccaccgagt
     2941 cggaatcgaa ggaaagcctt gatcgtttca ttcatgtcat gcgcgcgctt gccgaggaag
     3001 cgaaggccgg ccggctcgaa cgctttgccg gcgcgcccca tctcgcgccg cgccgccgcc
     3061 tcgacgaaac ccgcgccgcg cgcaaacccg tcctgcgctg gacgcagaac cccctgccgc
     3121 aagcggcgga atagaacaac gttctaccag acgcctggaa gcagcctgta gcgcacgcgg
     3181 cgggagtaat cggcgtagcc ttcgagcttc agccgcaatt cgcgatcttc catggcggtg
     3241 aggaacacga ttgcgctcgc aagcaccaaa acgcccgcca gtccgagcca agagcccaga
     3301 agaagggcgg tcgagacgaa aaggatgacg cctgccgcat aaagcgggtg acgaacaacc
     3361 gcgtaggggc cggtggaaat gatctgcctt ccgcgctcgg tccgctggcg cagggatcgc
     3421 cagagagacg tattttcctc gaagacgcga taaatggacg agagggcggc cgcgacgccg
     3481 atgccgcctg cgatctgcag ccagccgggc atccacgacc agtcgaaacg cacggcatcg
     3541 agccccatca gcgcgagcca gccggggaag aggacggtga acacaacctt gaaaatccca
     3601 tcccagggca gttccgcgcg aaggataggc cagcgcagtg aatttttcag gattgccgga
     3661 tcgtgccgcg cgaccatgcg ggtgatcaga aacgccacga ccagagagac accgacgaac
     3721 gcccaggccg gcggccagct caaggtgccc gccgcgccga acagcaccac ggcgatcgcc
     3781 gcctcgattc ccagcgcaac ccagaacttt gccgacatgc gccccgtcct ttccgagcgc
     3841 actgtaatac accatggggg agatttcgaa tcacttccgc gagcccgtac ggcaaaaatg
     3901 gggcgcgcgc cggcggtccg cggatggact ttcggcgtgc gccgttcatt c
//
LOCUS       DASZMC010000153         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_345484,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000153 DASZMC010000000
VERSION     DASZMC010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345484"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VG274_03015"
     CDS             <1..579
                     /locus_tag="VG274_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWE05651.1"
                     /translation="VSGYAVGHRSPMDAGSDHVQTDSGTPLTPLAVTAQAKPSAAQSS
                     FQPTAQPVLTDAPSLTGSDTAPAGASAETAVDVPVANSSGAGTSGESVVRTALPTQPG
                     ATPSIAGGSPRVEPALGQQPSIMVQIAAVSHAEDAEVLVGALRKRGYIVNARRDPTDG
                     LLHVQVGPFANRSDAVAMRQKLLNDGYNAIVQ"
     gene            complement(593..1303)
                     /locus_tag="VG274_03020"
     CDS             complement(593..1303)
                     /locus_tag="VG274_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWE05652.1"
                     /translation="MIASLTLLATLVILGTPTALVFIPWAMITGNATPLYNASQAIVR
                     LAFRLANIRVETSGEDRVPAHTACIFMSNHVSNLDPPALIPRIPGRTSAFLKRSLMKI
                     PLLGYGMKLGEFVPVDRDGRVQAAIESVNIARQVLEKGIHITTFVEGTRSRDGRLLPF
                     KKGPFYLAMETGAPCVPVSIHGTETMMAKGSLRVRPGTAHIRFHEPIDPHRFATREEL
                     MDAVRTAIASGLPESMRG"
     gene            complement(1318..>2506)
                     /locus_tag="VG274_03025"
     CDS             complement(1318..>2506)
                     /locus_tag="VG274_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWE05653.1"
                     /translation="VVERALDERIPFASMAALLHEEFLSGRESLVVAGTHGKTTTTSM
                     LSWIYQVAARENPAREPSFLIGGVAENFGSSFQLRPTRTFILEGDEYDTAFFDKGPKF
                     LHYFPDALILTHVEFDHADIYADLEAVKTAFKRLVNLVPRRGVIVAYDGSANVTECVS
                     RAFCRVERYGFSSDAEWRIQNLRHEDGLTRWEVWRDGAMWADLEMRLAGEHNALNATA
                     AAALAAGQGISGPAIQAALASFKSVKRRLEVRAEIGGVTIIDDFAHHPTAIRETLRAL
                     RSVYPEARLWAVLEPRSNTLRRKVLESEIVASLRLADRVILAGVYQQERIALDERLHP
                     EEVVAGLNASGTPAELCPDVETIIERIVPQLEAGDVVALLSNGGFDGIYEKLPARLRE
                     SLA"
BASE COUNT          483 a          735 c          819 g          469 t
ORIGIN      
        1 gtctccggct atgcggtcgg tcatcgtagc cccatggatg ccggttccga tcatgtgcaa
       61 acggactccg gaacaccctt gacgcccttg gctgtcacgg cgcaggctaa accttcggct
      121 gcgcaaagca gctttcaacc cacggcgcag cccgttctaa ccgatgcgcc gagcttaacc
      181 ggctcggata cggcgcccgc cggagcttcg gccgagaccg ccgtcgacgt gccggttgcg
      241 aactcaagtg gtgccgggac gtcgggggaa tcggttgttc gaaccgctct gccaacccag
      301 cctggcgcaa cgccatcgat cgccggtggc agtccacggg ttgaaccggc cctgggtcag
      361 cagccgtcaa tcatggtcca gattgccgcg gtctcacacg ccgaggatgc cgaagtgctg
      421 gtgggcgcct tgcgcaaacg aggatacatt gtgaatgccc gtcgcgaccc gacagacggg
      481 ctgctgcacg tgcaggtcgg cccgtttgcc aatcgaagcg atgctgtcgc tatgcggcaa
      541 aagctgctca acgacggcta taacgcaatt gttcagtagg aagtgatacc gctcagcccc
      601 gcatcgattc cggcagcccc gacgcaatcg ccgtacgcac ggcgtccatc agttcttcgc
      661 gtgtggcgaa gcggtgagga tcgatcggtt cgtggaaacg gatgtgcgcc gttccgggac
      721 gcacacgcag gctacctttt gccatcatgg tctcagtgcc gtggatggat acggggacgc
      781 agggtgcgcc ggtttccatg gcaaggtaga agggaccctt cttaaatgga aggagccgtc
      841 cgtcgcgaga gcgtgtgccc tcaacgaacg tggtgatgtg gatgcccttc tccaggactt
      901 gtctggcgat gttgacactt tcgattgccg cctgcacgcg gccgtctcga tcgacgggca
      961 caaactcgcc gagtttcatg ccataaccca gcagcggaat cttcatcaac gagcgtttga
     1021 ggaacgccga ggtgcgcccc gggatgcgcg ggatgagcgc cggcgggtca aggtttgaca
     1081 cgtgattaga catgaagatg caggccgtgt gggcgggcac gcggtcctcg ccggacgtct
     1141 ccacgcggat gttcgccaac cggaaggcaa ggcgcacaat cgcctgcgat gcattgtaga
     1201 gcggcgttgc gttcccagta atcatggccc agggaataaa gacgagtgcc gtcggcgtgc
     1261 cgaggatgac aagcgtagcc aacaatgtga gtgaggcgat cataagcagc tttcggctca
     1321 ggccaggctt tcccgaagcc gcgcgggcag tttctcatag atgccatcaa acccgccatt
     1381 ggagagaagg gcgaccacat cgcctgcctc aagttgcggt acgattcgct cgatgatggt
     1441 ttcgacatcg gggcacaatt cggccggcgt ccctgacgca ttgagtcctg ccacgacttc
     1501 ttcggggtgc aggcgttcgt cgagggcaat gcgctcctgc tgataaacgc cggccaggat
     1561 gactcgatcg gcgaggcgca ggcttgcgac gatttctgat tcgagcacct tgcgccgaag
     1621 ggtgttggaa cggggctcga ggaccgccca gagacgcgcc tcggggtaga ctgagcgcaa
     1681 tgcgcgcagc gtttcccgga tcgcggtggg atggtgcgcg aagtcgtcga tgatggtgac
     1741 gccgccgatc tcggcgcgca cttcaaggcg gcgcttgacg ctcttgaagc tggccaaggc
     1801 ggcttgaatc gctggcccgg agatgccttg tccagcggcg agagcggcgg cggcggtggc
     1861 gttcagcgcg ttgtgctcgc ctgccagacg catctcgagg tcagcccaca tggcgccatc
     1921 gcgccaaact tcccatcgtg taaggccgtc ttcgtgacga agattctgga tgcgccactc
     1981 cgcgtcgctg ctaaagccgt agcgctcgac gcggcagaag gcgcgggaca cgcactccgt
     2041 cacgttggcg ctgccatcgt aagcgacaat cacgccgcgg cgcgggacga ggttgacgag
     2101 gcgcttgaaa gccgtcttga ccgcttcgag atccgcgtag atatcggcat gatcgaactc
     2161 gacgtgggtc aggattaacg catcggggaa ataatgcaga aactttggtc ctttgtcgaa
     2221 aaaggctgta tcgtactcgt cgccctcgag aataaaggtg cgcgtgggac gaagctgaaa
     2281 gctgctgcca aagttttcgg cgacgccacc gatcaggaac gaaggctctc gcgcgggatt
     2341 ctcgcgtgcg gcgacctggt agatccatga gagcatgctg gtagtggtcg tcttgccgtg
     2401 cgtgccggcg acgaccagag attcgcgtcc actgaggaat tcttcatgga gcagcgcggc
     2461 catggaagcg aagggaatgc gttcgtcgag cgcgcgctcg accacg
//
LOCUS       DASZMC010000154         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3911816,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000154 DASZMC010000000
VERSION     DASZMC010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911816"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..889
                     /locus_tag="VG274_03030"
     CDS             275..889
                     /locus_tag="VG274_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05654.1"
                     /translation="MRGETFDFISNFSTLWQVVLGACLATAGGLAANQFEWRAQQQRR
                     ERNAALFFGEVLSSLAVILKLAHQTKQIGDPFGPITLRMLRSARREIDIYERNRESLI
                     DLRDANLRARIHRLTLRIAMPLDGMFDGSQEIALTEGQLRSRTLDDADRSELEARVAG
                     LKERRDAGYEYMCETSQEIAKILSDLAPLARHSFSYEDFALRSS"
     gene            1099..2700
                     /locus_tag="VG274_03035"
     CDS             1099..2700
                     /locus_tag="VG274_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE05655.1"
                     /translation="MAASCAGTVFEWYDFFIFGSLAAIIAAHFYSGAAASQGYIFALL
                     TFAAGFAVRPLGALVFGRFGDRRGRKRAFLVTITLMGLATVGVGLLPDYKSIGILAPY
                     VLVSLRVIQGFAIGGEYGGAAIYVAEHAAKRRRGAATGWIQVAASLGLVLALAVILVC
                     RTSFGEDSFRAWAWRIPFLISAGLLGISLWIRLRLDESPLFQRMQEEGRSSPAPLAES
                     FLRWSNLKIVLIALFGLLMGQGVVWYTAQFYTQFFLEGVVKVAPALVNSLIMAATVAS
                     AGLHMFFAWLSDKVGRKPVMLFGLGLAAVSFVPGFHLLAQSANPALTDAVARAPVTVV
                     ADPADCSLQFDLIGKARFTTSCDIAKGALAGAGVPYRNGAAPSGARALVRIGSDTVTS
                     PDGRGLDAHAIAAMKAKFLQALKLALRAAGYPASADSRALDIPRTLGLLLIFVIAAAA
                     LYGPQAAALVELFPTRIRYSALSFPYHVGVGWFGGFLPVTAYAIVVATGNIYAGLWYP
                     TIIAAIGFLVTLFFLPETRQRDIDA"
     gene            complement(2807..3283)
                     /locus_tag="VG274_03040"
     CDS             complement(2807..3283)
                     /locus_tag="VG274_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWE05656.1"
                     /translation="MERHKLDSIDIRILSELQSHGRITNVELSRRAKITAPPCLRRMR
                     ALEKAGYIKGYHAELDGKSLGYEVTGFAFVGLSSQAEADLKRFEEQVRSWPAVRECFM
                     LSGEVDFILKCVARDLSAFQSFITETLTAARNVASVKTALVIRASKNEPGIPLDAK"
     gene            3565..>4239
                     /locus_tag="VG274_03045"
     CDS             3565..>4239
                     /locus_tag="VG274_03045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWE05657.1"
                     /translation="MADATEAKVVILGSGPAGATAAIYAARAMLEPVLISGLQPGGQL
                     TITTDVENYPGFAEAIQGPWLMEQMQAQAEHVGTRVVQDMIANVDLGGHPFTLTGDSG
                     SRYRAQTIIIATGAQAKWLGLASEETYKGFGVSACATCDGFFFRGKRVAVVGGGNTAA
                     EEALFLTNFAEHVTLVHRGPVLRADKTNQMRLAANGKIAIRYDTVVDEIVGASEPRAV
                     NAVRLRS"
BASE COUNT          711 a         1343 c         1328 g          857 t
ORIGIN      
        1 gccctggtcg ctcccgagtg agcccgacat tccttcacgc cagaggcggc ccgattgagc
       61 atcccggctc tctgaagccg cctgaatcct tcgtgaggta tgaggattaa gcgccgctct
      121 cgcgaaggct cgggccgcaa agccgatcgc agggaaatcg ccaggcccct ataatccctc
      181 accgctttcg ccggacagcc atctggatcg cagcggacac cgcctctagc atggaccatc
      241 ggaatcgttc cacgagacgc ccttcgggat tacgatgcgc ggcgagactt tcgatttcat
      301 ttcgaacttc agtaccttgt ggcaggttgt gctgggcgca tgtctcgcca cggctggcgg
      361 gctcgccgcc aatcagtttg aatggcgtgc ccagcagcaa cggcgcgagc gcaatgcggc
      421 gctgtttttc ggcgaagtgc tttcgagctt ggccgtcatc ctgaagctgg cgcaccagac
      481 caagcagatc ggcgacccct tcggccccat cacgctgcgc atgctgcggt cggctcgacg
      541 cgagatcgac atttacgagc gcaaccgcga gagcctgatc gatttgcgcg acgccaattt
      601 gcgggcgcgc attcacaggc tgactctgcg gatcgccatg ccgctcgacg gaatgttcga
      661 cggctcgcag gaaattgcgc tgaccgaagg tcagttgcga tcccgcacgc tcgacgatgc
      721 ggaccgcagc gagctggaag cacgagtggc tgggctgaag gaacgccgcg acgccggtta
      781 tgagtatatg tgcgagacgt cccaggaaat cgcgaaaatt ctttccgatc tggccccgct
      841 tgcccgacat tccttcagct acgaggactt tgccctccgc agcagctgac gcgggttcgc
      901 ccctgcgccc atgtcccgcc gccctcttcg ccgcccagta ccgggggacc agctcaagca
      961 tgaggccctc atggtgagct tgtcgaacca tgaaggccgg ggcacatagg gagcgcccaa
     1021 ggagcaaaaa cgcgaatgtc ggcggtcgag gctggggcgc ggccggttgg ctccgccggg
     1081 agaaacttaa cgcttgtggt ggccgcgtcc tgcgcgggca cggttttcga gtggtacgat
     1141 ttcttcattt ttggatcgct tgcggcgatc attgcagccc acttctattc cggcgccgcg
     1201 gcgtcccaag gctacatctt cgctcttctg acgttcgccg ccggcttcgc cgtgcgccca
     1261 cttggggcac tcgtgttcgg ccggttcgga gatcgtcgag ggcgaaagcg cgcctttctg
     1321 gtcacgatca cgctgatggg cctggcgaca gtcggcgtgg gactcctccc cgactacaag
     1381 tcgatcggaa tcctggcgcc ttatgtgctg gtttcgctgc gcgtcatcca gggttttgcc
     1441 atcggcggcg aatatggcgg ggcggcgatc tacgtggcgg agcacgctgc aaagcgccgg
     1501 cgaggcgccg cgacaggctg gatccaggtc gcggcatcat tggggctggt tcttgcgctg
     1561 gcggtgattc tcgtctgtcg cacctcgttc ggcgaagatt cattccgggc ctgggcgtgg
     1621 cgaatcccct ttctgatttc ggcaggcctg ctgggcattt ccctgtggat caggctgcga
     1681 ctcgatgaat cgccgctgtt ccagcgcatg caggaggaag gtcgctcgtc cccggcgccg
     1741 ctcgccgaat cgttcttgcg gtggagcaat ctgaagatcg ttctgatcgc tcttttcggt
     1801 ctgctcatgg gccagggcgt cgtctggtac acggcgcaat tctacacgca gttctttctg
     1861 gaaggcgtgg tcaaggtcgc gccggcgctg gtgaactccc tcatcatggc cgccacggtc
     1921 gccagcgcgg gattgcacat gttcttcgcc tggctgtccg acaaggtggg ccgcaaaccc
     1981 gtaatgctgt tcggactggg gctggccgcc gtcagcttcg ttcccggctt ccacctcctg
     2041 gcccagtccg ccaaccctgc actcaccgac gcggttgcgc gcgcgccggt caccgtcgtc
     2101 gccgatcccg cggattgctc gctgcaattc gacctgatcg gcaaggcgag gtttacgact
     2161 tcctgcgaca tcgccaaagg cgcacttgcc ggcgccgggg tgccctatcg aaacggggct
     2221 gcgccttcgg gcgcgcgggc attggtgcgg atcggcagcg acaccgtgac cagtccggac
     2281 gggcgcggac tggatgcgca tgcgatcgca gccatgaaag cgaaattcct tcaggcgctg
     2341 aaactcgcac tccgtgcggc gggctatccg gcttctgcag acagtcgcgc actcgacatt
     2401 ccgcgcacgc tcggcctgct gctgattttc gtcatcgccg cggccgcgct ctacggcccg
     2461 caagccgccg cactggtcga gctctttccg acgcgcatcc gctattccgc gctctcgttt
     2521 ccctatcatg tcggggtggg ctggttcggc ggatttctgc cggtgaccgc ctatgcgatc
     2581 gtggttgcaa ccggaaacat ctatgccggc ctctggtacc cgaccatcat cgcggcgatc
     2641 ggattcttag tcacgctttt cttcctgccc gaaacgcgcc agcgggacat cgacgcatag
     2701 atcccgttcg atcttggcag tcgctgctcc tcatgcttcg actggctcag catgaggttt
     2761 ctcacaatcc tcaccctgat ttcgtcgaag ggtgagtcac atgctctcac ttcgcatcaa
     2821 gcggaatacc cggctcgttc ttgctggcgc ggatgacgag tgccgtcttg acgcttgcga
     2881 cgttcctggc cgcggtcagc gtttcggtga tgaacgactg aaacgccgat agatcgcgcg
     2941 ccacgcattt cagaatgaaa tcgacctctc ccgagagcat gaaacattcg cgtacggcgg
     3001 gccagctgcg cacctgttct tcgaaccgct tcaggtccgc ttcggcctgg cttgagagcc
     3061 cgacgaaggc gaagcccgtg acctcgtagc ccaaagactt gccgtcaagc tcggcatggt
     3121 agcccttgat atatccggcc ttctccagcg cccgcatccg tctcaggcac ggtggcgcgg
     3181 tgattttggc gcggcgggaa agttccacat tggtgatgcg gccatgactc tgcaattccg
     3241 aaagaatgcg gatgtctatg gagtcgagct tgtgacgctc catgatgaaa ccttcgacag
     3301 gggatgcggc ggcatttaat acttggcgcc ctccccgcgc aataatattt cgggatcgca
     3361 cattttcgtg tccaccgatt cgggcatggc aaattggtgg aattcctcgg tccgcccaag
     3421 atggcgaggg tcccaaggat tgagcggtgt tcgcggccgt gcttgacctt tcagcccagt
     3481 gttccgatat cggaaggaac tgggctgtca ggccgcggcg tgcgcggcgg gttcaccctc
     3541 gcggttcacg cattcgggag cggcatggcg gacgcgacag aagccaaggt tgtcattctg
     3601 ggcagcgggc cggccggagc gacggctgcc atctatgcgg ctcgcgccat gctcgaaccg
     3661 gtgctgatct cgggcctgca gccggggggg cagctgacca tcacgaccga cgtcgaaaac
     3721 tatcccggct tcgcggaagc gatccagggg ccctggctca tggagcagat gcaggcgcag
     3781 gccgagcatg tgggcacccg ggtcgtgcag gacatgattg cgaatgtcga cctcggagga
     3841 caccccttca cactgaccgg cgattcgggc tcacgctatc gggcgcagac catcatcatt
     3901 gccaccgggg cgcaggccaa atggctgggc ctcgcctcgg aagaaaccta caagggattc
     3961 ggcgtatcgg cctgcgcgac ctgcgatggc ttctttttcc gcggcaagcg cgtcgccgtc
     4021 gtcggcggcg gcaacacggc ggcggaagag gcgctcttcc tcaccaattt tgccgagcac
     4081 gtcacgctgg tccatcgcgg gccggtgctg cgcgccgaca agaccaacca gatgcgcctc
     4141 gcggccaatg gaaaaatcgc catccgctat gataccgtcg tggacgagat tgtgggcgcg
     4201 agcgagccgc gcgcggtcaa tgctgtgcgg ctgcgcagt
//
LOCUS       DASZMC010000155         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1978018,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000155 DASZMC010000000
VERSION     DASZMC010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978018"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VG274_03050"
     CDS             complement(<1..287)
                     /locus_tag="VG274_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWE05658.1"
                     /translation="MRENVVSLNRNPLDALALPKFEGGSVWLAGAGPGDPGLITALGI
                     HALQNADLVLHDALIDETLLKLARPGAEIIYAGKRGGKKSCKQSDISRTLVS"
     gene            433..1932
                     /locus_tag="VG274_03055"
     CDS             433..1932
                     /locus_tag="VG274_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWE05659.1"
                     /translation="MSDKIRSDLKPNTFEYEMQPLITQNGFREYDARWLFGKELNLMG
                     VQMVGLGLGTYFHELGIKPKVAVGHDYRSYEMSIKQALIIGLMQAGCEVVDIGLGTTP
                     MGYFAQFALDCPGLAMVTASHNENGWTGVKMGAQMPLTFGPTEINRLKAIVLGEEFKP
                     RAGGSYSRVENFRDKYLDELTKGHKLSRPMKVTVACGNGTAGLFAPEALRRIGAEVTE
                     SECNPDYNFPNYNPNTEDLHMLDAMAKDLKANGSEIALGFDADGDRCGVVDDKGEEIF
                     ADKIGVLLARDMSTARPGAKFVVDVKSTGIFDIDPILKKNGATTDYWITGHSYMKRRC
                     KEIGALCGFEKSGHYYLCEPLGRGYDDGIVAGIAVLEMLDRAGSKKMSDLVASLPKTW
                     GSPTMAPECPDDKKYGVVDAMVKEYQDAEKNGEQILGQKIKKLVTVNGVRVTLEDGTW
                     GLVRASSNKPSLVVVVESPVSDAQMHAMFKDLDGRLSKHSEVRAYDQKI"
     gene            2035..2679
                     /locus_tag="VG274_03060"
     CDS             2035..2679
                     /locus_tag="VG274_03060"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HWE05660.1"
                     /translation="MPDIDEYKGTHLTILNEGKRCIHSRHCVLNLPHVFKPNVDGPWI
                     DPDAANVEATIAVITKCPSGALRYRPEGAMPAEGAPGVNTIRTWEDGPLAVHAELDVA
                     GDTSSRRATLCRCGQSFNKPYCDKMHEKAGFKATGEPASGEAETLAVKNGPLKVTPTK
                     DGPLQVEGALEICSASGRTISRTMETWLCRCGHSANKPFCDGSHAKVGFTAPGS"
     gene            complement(2682..>3191)
                     /locus_tag="VG274_03065"
     CDS             complement(2682..>3191)
                     /locus_tag="VG274_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWE05661.1"
                     /translation="AGTGPAMTKKGMAVKREESARLDLDRFVPYKLSVLSNRVSDAIA
                     RHYSERFGLSIPEWRVMAVLGQTPGLSARDVAARTAMDKVQVSRAVASLMHAKRIARV
                     GDAKDGRITRLSLTRQGTAIYDEIVPLALHLEQLFLAALTPAERTTLATLMNKLATQV
                     NGLHISAMR"
BASE COUNT          668 a          964 c          989 g          570 t
ORIGIN      
        1 gagacaagcg tgcgcgagat gtcgctttgc ttgcaggatt tcttcccgcc acgtttaccg
       61 gcgtagatga tctctgcacc cggccgcgcg agtttgagca gcgtctcgtc gatcagcgcg
      121 tcgtgcagca ccaaatccgc attctgcagc gcgtggatac ccagcgccgt gatcaacccc
      181 ggatcgccgg ggccggcgcc ggcgagccaa acgctgccac cttcgaattt cggcaacgcc
      241 agtgcgtcca gcggatttcg attcagcgaa acgacgtttt cgcgcatttc actctctcat
      301 tctgtgaata aaattgtatc acacattcat aatgtaggta gcccgccctg aaattaaaga
      361 aatccgcaaa cgccgggact tttgccggtt ccagcggtta tagaggctcc cgcaaacaga
      421 gccccaaacc ccatgtccga caaaatccgc agcgacctga agcccaacac cttcgaatat
      481 gagatgcagc cgcttatcac ccagaacggc ttccgcgaat acgacgcgcg ctggctgttc
      541 ggcaaagagc tgaacctgat gggcgtgcag atggtggggc tgggcctcgg cacctatttc
      601 cacgagcttg gcatcaagcc gaaggtcgcg gtcggccacg actatcgctc ctacgagatg
      661 tcgatcaagc aggcgctgat catcgggttg atgcaggcgg gctgcgaggt cgtcgatatc
      721 ggtctcggca cgacgccgat gggttacttc gcgcaattcg cgctcgattg tcccggcctt
      781 gcgatggtca ccgccagcca caacgagaac ggctggaccg gcgtgaagat gggcgcgcag
      841 atgccgctca ccttcggccc cacggagatc aatcgcctga aagccatcgt actcggcgaa
      901 gaattcaagc cgcgcgccgg cggcagctat agccgtgtcg aaaacttccg cgacaaatat
      961 ctcgatgagc tcaccaaggg ccacaagctg tcgcgcccga tgaaggtgac ggtcgcgtgc
     1021 ggcaacggca ccgccggtct gttcgcgccg gaagcgctcc gccgcatcgg tgccgaggtg
     1081 acggagtcgg aatgcaatcc ggactacaat ttcccgaact acaatcccaa caccgaagac
     1141 cttcacatgc tcgacgccat ggcgaaggat ttgaaagcga acggcagcga gattgcgctc
     1201 ggcttcgatg ccgatggtga tcgctgcggc gtggtggacg acaagggcga ggagattttt
     1261 gccgacaaga tcggcgttct gctggcgcgc gacatgtcga cggcacggcc cggcgcgaaa
     1321 ttcgtcgtcg atgtgaagtc gaccggcatc ttcgacatcg atccgatcct gaagaaaaac
     1381 ggcgccacca ccgattactg gatcaccggc cattcctaca tgaagcgccg ctgcaaggag
     1441 attggcgcgt tgtgcggctt tgagaagagc ggccactatt atctctgcga gccgctcggc
     1501 cgcggttatg acgatggcat cgtcgccggc atcgcggtgc tggagatgct cgaccgcgcc
     1561 ggatcgaaga aaatgtcgga cctcgtcgcc agcctgccca agacctgggg ctcgccgaca
     1621 atggcgcctg aatgccccga tgacaaaaaa tacggcgtcg tcgacgcgat ggtgaaggaa
     1681 tatcaggacg ccgaaaagaa cggcgagcag attctcggtc agaagatcaa aaagctcgtc
     1741 accgtcaacg gcgtgcgcgt gacgctggaa gacggtacgt ggggtctggt gcgcgcgtcg
     1801 tccaacaagc cctcgctcgt cgtcgtggtc gaaagcccgg tctccgacgc ccagatgcac
     1861 gcgatgttca aggacctcga tgggcggctg tcaaagcatt cggaagttag ggcgtacgac
     1921 cagaaaattt gaggccagag ctgccccttc atccttcgac aagctcagga tgaagggttt
     1981 aagctcatcc tcaccctgag cttgtcgaag ggtgaggaag cgcgaaggcc tgccatgccc
     2041 gacatcgacg aatacaaagg cacgcatctc accattctca acgaaggcaa gcgctgcatt
     2101 cattcgcgcc attgcgtgct gaatctgccg catgtgttca agccgaatgt cgatgggccg
     2161 tggatcgatc ccgatgcggc gaatgtcgag gcgacgatcg cggtcatcac gaaatgtccg
     2221 tctggcgcgc tgcgctatcg gccggagggc gcgatgccgg cggaaggcgc gccgggcgtc
     2281 aacacgatcc gcacatggga ggacggcccg ctcgcggtgc atgccgagtt ggatgttgca
     2341 ggcgatacat cgagccgccg cgccacgctg tgccgctgcg gccagtcgtt caacaaacct
     2401 tattgcgaca agatgcatga gaaagccggc ttcaaggcaa cgggcgagcc cgcgagcggg
     2461 gaggcggaaa cgctcgccgt gaagaacggt ccacttaaag tcacgccgac aaaggatggg
     2521 ccgcttcagg tcgaaggggc gctggaaatc tgctccgcca gtggccgcac gatttcgcgc
     2581 acgatggaaa cctggctctg ccgctgcggc cattcggcca acaagccgtt ctgcgacggc
     2641 agccatgcga aagtgggttt caccgcgccg ggcagctgag gtcagcgcat cgcggagatg
     2701 tgaaggccgt tcacttgcgt cgcgagcttg ttcatcagcg tggcaagcgt ggtgcgctcg
     2761 gcgggcgtca gtgcggcgag aaagagctgc tccagatgca gcgcgagcgg cacgatctcg
     2821 tcatagatcg cggtgccttg ccgcgtgagc gagaggcgcg tgatgcggcc gtctttcgcg
     2881 tcgccaacgc gcgcgatgcg cttggcgtgc atgagcgacg ccaccgcgcg gctgacctgc
     2941 accttgtcca tcgccgtgcg tgcggcgaca tcacgcgcgg aaagacccgg cgtctggccg
     3001 agtactgcca tcacgcgcca ttccggaatc gaaagaccga agcgctcgga ataatgccgc
     3061 gcgatggcat ccgacacgcg gttcgagagt accgacagct tgtacggaac gaagcgatcg
     3121 agatcgagac gcgcggactc ttctctttta accgccattc cctttttcgt catggccggg
     3181 cctgtcccgg c
//
LOCUS       DASZMC010000156         4756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3369703,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000156 DASZMC010000000
VERSION     DASZMC010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3369703"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="VG274_03070"
     CDS             complement(<1..1812)
                     /locus_tag="VG274_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWE05662.1"
                     /translation="MALPIDVLIPEILASLRRSPNLVIEAAPGAGKTTRVPPALLQLG
                     GGVLVLEPRRIAARMAARRVAAEMGEPVGETVGYQVRFEETAGPRTRLRFLTEGVLTR
                     RLIRDPELRGVNTVILDEFHERHLETDLALALLRRLQKTKRPDLRLVVMSATLDADPV
                     REFLGGCPRLESEGRLFDLTIDWTPYSAAPLENQVAAALESVIARNIPGDVLVFLPGA
                     AAIHRATRACDPIARRHNLLLTQLYGDLPPAEQDRALEPSAQRKVILSTNIAESSITI
                     DGVRIVIDSGLARIAADSPWTGLPSLEIGRISKASATQRAGRAGRTASGIVIRLYTAE
                     DFHRREDHDAPEIVRRELSQMCLQLESMGTHDPTAMEWLTPPPAASLQASENLLDRLH
                     ARGADAEKLAAMPLHPRLGRLMLDAERADIGDSGCRVAALLSSGQRVRSTDLFVALDE
                     ERDPRTRAVYEQLRRHVRPGKTKHNDEALAKAVLAAFPDRVGRRRPNNVVLLSNGSSA
                     TMSGMSGDIPQNGEFLVAIDAEANLLRLACRIEPEWLIDVFPGSLTERLQLEWNRQSE
                     RVESVSALLYDELVIEETRGGKIDDEEAAKLLATKASE"
     gene            1903..2745
                     /locus_tag="VG274_03075"
     CDS             1903..2745
                     /locus_tag="VG274_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWE05663.1"
                     /translation="MSLTRRTFLGSAVAAAGAPVVSAQSKSIPIGLELFSVRSALSAD
                     PTGTLKKVAAIGYQVVEFYAPYYAWTLDQAKDTKKLLDDLGIKCNSTHNNLVSFQPAN
                     LQKTIDLNHAIGGEFVVLASSSQTKDPDYWKKLALETLTPAADTLRKAGLRAGYHNHQ
                     YEFVPTNNNGPRAMEILAANTPKDFMLQLDVGTCVEVGGDPVAWVNANPGRINSMHLK
                     DWNKERGYRVLFGEGSVQWKPLLAAAEKTGGAEYLLIEQEGSDYSEFETAQRCLDNYR
                     KMVG"
     gene            2807..3388
                     /locus_tag="VG274_03080"
     CDS             2807..3388
                     /locus_tag="VG274_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05664.1"
                     /translation="MPLALSARSAFAQVIEYESNGEKYQTLTRAGLTVIFTHLPAQVA
                     GFGLLQVSIANGSQIHWTVKPEQFEYVRAGETIIATPADDVVNTMMARASTNDVIKLT
                     TSYERSLYAIPNMRSNNGYEQRRQNALAFGGSPKIKAAAAASAITLAQIRLAPGDSTD
                     GAVFLKLTKDLKTLTGGHVEIHVENETFQFNPD"
     gene            complement(3385..4152)
                     /locus_tag="VG274_03085"
     CDS             complement(3385..4152)
                     /locus_tag="VG274_03085"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWE05665.1"
                     /translation="MPNFKVLIVDDERLSRRRVRRLLSLEPDCEVSGECANGAEAVTS
                     LKQSRPDIIFLDVQMPEMDGFEVARAMADLKPLVIFTSAYDEYALRAFEVQAFDYLLK
                     PFDGRRFRESLQRAKVRVEIDRSGQHDRRPAPAEQPMPRVAPDRIAVRNNGRVVFVKL
                     TDIDWIEAADNYVCLHCGRETHVVRETMNELEARLDPAQFLRVHRSSIVNLDRVRELQ
                     PWFRGDYRVVLRDGTQLTLTKNHREKLESRLLLGVWH"
     gene            complement(4121..>4756)
                     /locus_tag="VG274_03090"
     CDS             complement(4121..>4756)
                     /locus_tag="VG274_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWE05666.1"
                     /translation="AQLQALKIQLHPHFLFNTLHSITALVHEDPEMAERTIARLSELL
                     RLFLANSAIHEIPLSEELRILDLYLEIERARFEDRLRVMYDVPSNLRDAMVPNLILQP
                     LVENSIRHGVGRRSEPGWIAVAVERYADTLIVRVTDNGAGFKEDPPVHTGMGLSITRG
                     RLESLYGSHQSLVLRNLPAGGVEARITMPFHVQTTSTGNEAHAELQSTDRG"
BASE COUNT          921 a         1267 c         1631 g          937 t
ORIGIN      
        1 ttcggacgct ttggtggcca ggagttttgc cgcttcttca tcatcgattt tgccgcctct
       61 ggtttcttcg atcacgagct cgtcgtacag gagcgcgctc acggattcga ctcgctcgga
      121 ttgacggttc cattcgagct gaaggcgttc cgtgaggctc ccggggaaaa catcgatgag
      181 ccattcgggt tcgatgcggc aggccaggcg gagcagattc gcttccgcgt caatcgcaac
      241 taagaattcg ccgttctgcg gtatatcgcc tgacatgccg gacatggtgg cggaactgcc
      301 gttcgagagc agaacgacat tgttgggtcg acggcgcccg acgcgatcgg gaaaggcagc
      361 cagcacggct ttggcgagag cttcgtcgtt gtgtttggtt ttacccgggc ggacatggcg
      421 gcggagttgc tcgtagacgg cacgtgtgcg cgggtcacgt tcttcatcga gggcgacgaa
      481 gaggtccgtg gatcgtacgc gctggccgct gctgagaagt gcggcgacgc ggcatccgga
      541 gtcgccgata tcggcgcgtt cggcatcgag catcaggcgg ccgagccttg gatgcagcgg
      601 catggcggcg agcttttccg cgtcggctcc gcgggcgtgg aggcgatcga gcaggttttc
      661 ggaagcctgt agtgaagcgg cgggcggagg cgtgagccat tccattgcgg tgggatcatg
      721 agtgcccatg gattcgagtt gcaggcacat ctgcgacagc tcgcggcgaa caatttccgg
      781 cgcatcgtga tcttcgcggc gatgaaaatc ttcggcggtg tagaggcgga tgacgattcc
      841 ggatgcggtg cgtccggcgc gtccggcgcg ttgtgttgcg gaggctttgc tgatgcggcc
      901 gatttcgagg gaggggaggc cggtccacgg cgaatcggcg gcgatgcgcg cgaggccgga
      961 atcgatgacg atgcgaacgc cgtcgatggt gatggagctt tcggcgatgt ttgtcgagag
     1021 aatcactttg cgctgagcgg atggttcgag ggcgcgatcc tgttcggcgg gtgggagatc
     1081 gccgtagagc tgcgtgagca gaagattgtg gcggcgcgcg atgggatcgc acgcgcgtgt
     1141 ggcgcgatgg attgcggctg cgcctggaag gaagacgagg acgtcaccgg ggatgttgcg
     1201 ggcgatcacg gattcgagcg cggcggcgac ctgattttcg agcggcgccg cggagtaggg
     1261 agtccaatcg atggtgagat cgaagaggcg gccttcggat tccaggcgcg ggcaaccgcc
     1321 gagaaattcg cgcacgggat cggcatcgag cgtggcggac atgacgacga ggcggaggtc
     1381 ggggcgtttg gttttctgaa ggcggcggag gagcgcgagc gcgagatcgg tttctagatg
     1441 gcgctcgtga aattcgtcga ggatgacagt gttgacgccg cgcagttcgg gatcgcgaat
     1501 gaggcggcga gtgagaacgc cttcggtgag gaagcggagg cgtgtgcggg ggccggcggt
     1561 ttcttcgaag cgcacctgat agccgacggt ttcgccgacc ggttcgccca tttcagcggc
     1621 gacgcggcga gcggccatgc gggcggcgat acgtcgtggt tcgagtacga gaacgccacc
     1681 gccgagttgg aggagagcgg gtggaacgcg ggttgttttg ccggcgccgg gggcggcttc
     1741 gatgaccaga ttcggcgagc gccgcaggct ggcgagaatc tccggaatta agacatcgat
     1801 cggcagcgcc acttcactta ttgtctacgt gtttatgttt cgttcatacg gtttcaattc
     1861 gctatcgcaa gggccgtaaa tgtgctattg ttgctttcga atatgtcact cacccgcaga
     1921 acatttctgg gcagcgcggt tgcggcggct ggcgcgccgg tggtttcggc acaaagcaag
     1981 tcgatcccga tcgggctgga gcttttctcg gtgcggtcgg cgctatcggc agatccgacg
     2041 ggcacactga aaaaagttgc ggccattgga tatcaggtgg tggaatttta cgcgccgtat
     2101 tacgcctgga cgctcgatca ggcgaaggat acgaaaaagc tgctcgacga tctgggcatc
     2161 aaatgcaact cgacgcacaa caacctcgtg agcttccagc cggcgaatct gcagaagacc
     2221 atcgatctga atcacgcgat cggcggagaa tttgtggttc tggcgagttc atcccagaca
     2281 aaggatccgg actactggaa aaaactggcg ctcgagacgc tgactccggc ggcggatact
     2341 ctgcggaagg ccggcctgcg cgccggttat cacaaccacc agtacgaatt cgttcctacc
     2401 aacaacaacg gcccccgcgc catggaaatc ctcgcagcca acacgccgaa ggatttcatg
     2461 ctgcagctcg acgtgggtac gtgcgtggag gtgggcggcg atccggtggc gtgggtgaac
     2521 gcgaatccgg ggcgcatcaa ttcgatgcac ctgaaggact ggaacaaaga gcgcggctat
     2581 cgcgtgctgt tcggcgaagg atctgtgcag tggaagccgc tgctggcggc tgccgagaaa
     2641 accggtggcg cggaatatct gctgatcgag caggaaggca gcgattactc ggagtttgag
     2701 acagcgcagc gctgcctgga caactatcga aagatggtgg gttagtccgc gcacgcgcgt
     2761 tcggagttgc atggcatcgc gaatttcccg tagaggcttc ctcgcagtgc cgcttgcgtt
     2821 gtccgcgcgc agtgcgttcg cgcaggtcat tgagtatgaa tcgaacggcg agaagtacca
     2881 gacgctgacg cgagccggcc tcacggtaat tttcacgcat ctgccggcgc aggtagcggg
     2941 cttcggactg ttgcaggttt cgattgccaa cggctcacag attcactgga cggtgaaacc
     3001 ggaacagttc gagtatgtgc gggcagggga aacgatcatc gcaactccgg cggacgatgt
     3061 ggtgaatacg atgatggcgc gcgcgtcgac taacgacgtg atcaagctga cgacctcgta
     3121 cgagagatcg ctttatgcga ttccgaacat gcgctcgaac aacggttacg agcagcggcg
     3181 gcaaaacgcg ctggcgttcg gaggatcgcc gaagatcaag gctgcagcgg cggcttccgc
     3241 aattaccctg gcgcagattc ggctcgcgcc cggtgactcg acggatggcg cggtgtttct
     3301 gaagctgacg aaggatttga aaacgctgac cggcgggcac gtggaaatac acgtggagaa
     3361 cgagactttc caattcaacc cggattagtg ccagacgccc agcagtaatc tcgattcgag
     3421 cttttcgcga tggttcttcg tcagcgtgag ttgcgtgcca tcgcgcagca cgacacgata
     3481 atcgccgcgg aaccatggct ggagctcacg gacgcggtcg agattcacga tggaggaccg
     3541 atgcacgcgg aggaactgtg cgggatcgag gcgcgcttcg agctcattca tggtttcgcg
     3601 cactacgtgg gtttcgcgtc cgcaatgcag gcaaacgtag ttatccgcag cttcaatcca
     3661 gtcgatatcg gtgagcttga caaaaacgac gcggccgttg ttgcgaacgg cgattctgtc
     3721 cggcgcaacg cggggcatcg gctgttcggc aggcgcgggg cggcggtcgt gctggcctga
     3781 acgatcgatt tcgacacgga ctttcgcgcg ctggagcgac tcgcgaaaac ggcggccatc
     3841 gaacggcttc agcagataat cgaacgcctg cacttcgaag gcgcggagag cgtattcgtc
     3901 gtacgcggaa gtgaaaatca cgagcggctt gaggtcggcc atggcgcgcg cgacttcgaa
     3961 gccatccatc tccggcatct ggacgtcgag gaagatgata tccggacgtg actgcttcag
     4021 cgaggtcacc gcttccgcac cgttggcgca ttcgccggag acttcgcaat ccggctcgag
     4081 gctcagcaga cggcgaacgc gccgccggga gagccgttca tcatccacga tcagtacttt
     4141 gaagttcggc atgtgcttca ttccccgtcg atgtcgtttg cacgtggaag ggcatcgtga
     4201 tgcgcgcttc cactccgccc gccggaaggt tccggagcac cagcgactgg tgcgatccat
     4261 agagcgattc cagccggccc cgcgtgatgc tcaggcccat accggtgtgg accggaggat
     4321 cttctttgaa tccggcgccg ttgtctgtta ctcgtacgat cagtgtatcg gcatagcgct
     4381 cgacggcgac agcgatccag ccgggttcgg agcggcggcc cacgccgtgg cgaatcgaat
     4441 tctcgaccag cggctgcagg atcaggttcg gcaccatggc gtcgcggagg ttcgacggca
     4501 cgtcatacat gacgcggaga cggtcttcaa aacgcgcgcg ctcgatttcc agataaagat
     4561 cgagaatgcg cagttcttcg ctgagcggga tttcgtgaat ggcgctgttg gcgagaaaga
     4621 gacgcagcag ttcgctcagc cgcgcaatgg tgcgctcggc catttccgga tcttcatgga
     4681 cgagcgcggt gatgctgtgc agcgtgttga agagaaagtg cggatgcaat tgtatcttga
     4741 gcgcctgcag ttgcgc
//
LOCUS       DASZMC010000157         4101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3369730,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000157 DASZMC010000000
VERSION     DASZMC010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3369730"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VG274_03095"
     CDS             <1..195
                     /locus_tag="VG274_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05667.1"
                     /translation="TESTHVTERYHRVDFGHLELAVTIDDPTSYTKPWSVTEHFDYLP
                     NTEIIEDVCENEQDVKHMPK"
     gene            complement(345..1454)
                     /locus_tag="VG274_03100"
     CDS             complement(345..1454)
                     /locus_tag="VG274_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWE05668.1"
                     /translation="MKRNSKQIGLAIVGAGRVGLFRGAVAARHGSVGWIGIAEKRPER
                     AEAVAKEIGADFVTSDHRELLSRPEVNAAIIATDEHLHVDPVMCGVERGIPLMIEKPL
                     ATKIAESAKVLEAIEKSGIDAVVGYTQRFRRRWLAAKEKVRTGALGDVTLVTSRAFMN
                     RLVAIDNYQRSDAPETISPLVISGTHALDIAMWYLEAKTPVEVYARSVDKVLGPLYKG
                     IDATAGMIMFSDGCVYHLSISWALPVTWPAAVYSLEVGIVGTTGVLTIDDTHRDIVLA
                     VSAPQSEGYAPDKSRLVDFMGSYPPGDMALGELRGPMREETESWLNRVALGVATPHAT
                     AREAHNRLMLTKALDLSARRKAPVELPLKAGDDLI"
     gene            1837..3102
                     /locus_tag="VG274_03105"
     CDS             1837..3102
                     /locus_tag="VG274_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE05669.1"
                     /translation="MRTRTNVRWWICFFLFLTWLVSYIDRSLMPMALPFIGQEFHLSP
                     TVMGAVISAFFVGYATMQIPGGMLADRIGPRNSITLGIAFWSGFSVLTGLAGNLASLL
                     AVRVGFGLAEGLHPAAAFKALSAWFPAHERSRANGLVMSSNALGPMIAPVLFASLMSA
                     FGWRVAFYIVSVPGLLVAVGAYWLLRDRPAQHPRVTPEELAEIGNDEESQPKPPFREL
                     LKYPVLWRLFAIYMLWDITWWGFQAWLPSYLFKVRGFSLAHTGLVTALPYAAGFVGVT
                     LAGWIADRTRKREAVLAVVLVGIATFMVLTATAPTAVTAVIFLTAAGFFLPAVHGPFW
                     SLLMEMLPSRVMGYSSGFINTGGQIAGILAPIVIGALIQWTTHYYAGFIFMAISATIS
                     ALLVATLPRTASSHPPKIAPVVRERVQSR"
     gene            complement(3215..3907)
                     /locus_tag="VG274_03110"
     CDS             complement(3215..3907)
                     /locus_tag="VG274_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWE05670.1"
                     /translation="MSDPGCVRPNNEDCYLLAPAMGLYVVADGMGGAQAGEHASHMAV
                     DTVKEIVAMSASRGPAVLQTAFAEANTRVSMAAEHDPELDGMGTTLIAVLDNTTELFV
                     ASVGDSRVYSFQDGALHTITEDQSWVNEVGRRLGLEETVLKTHPMRHVLTMAIGVSDQ
                     LRVQTYRVEPKPGMLVLLCSDGLHGVVPEENLAATMADGGTLEEKCRSLVAIAKEHGG
                     PDNITAVLLKVV"
BASE COUNT          758 a         1423 c         1086 g          834 t
ORIGIN      
        1 accgaatcaa cgcacgtgac cgagcgctat catcgcgtcg acttcggcca tctggaattg
       61 gcggtgacca tcgacgatcc cacgtcttat acgaaaccgt ggagcgtcac cgagcatttc
      121 gattatttgc caaacaccga aatcatcgaa gacgtgtgcg agaacgagca ggacgtcaag
      181 cacatgccga aatgacgacg tggcgcagac cgccaggtct gctgagtcgg cagtcgtgcc
      241 gacttttccg aagctaactt acaagggaac tgtcgagagg attctcgact cggcaggccc
      301 tgcgcgtata aaggccctgc gtgtagagac gtcgcccgcg caaatcaaat gagatcgtcg
      361 cccgccttca gcggcaactc caccggagcc ttccgcctcg ccgacaaatc caaagccttc
      421 gtcagcatca accgattatg cgcctcgcgc gccgtcgcgt gcggcgtcgc cacgcccagc
      481 gccacgcggt tcagccatga ctccgtctcc tcgcgcatcg gcccgcgcaa ctcgccgaga
      541 gccatatcgc ccggaggata actgcccata aaatccacca gccgagattt atcgggagca
      601 tacccttccg attgcggcgc gctcaccgcc agcacaatat cgcgatgcgt atcgtcgatc
      661 gtcaacacgc ccgtcgtgcc gacgatcccg acctccaaac tataaacagc ggcgggccac
      721 gtcaccggca gcgcccacga gatgctcaag tggtacacgc acccgtcgct gaacataatc
      781 atgccggccg tcgcgtcgat gcccttgtac agcggcccca gtaccttatc gacggagcgc
      841 gcatacacct ccactggcgt cttcgcctcc agataccaca tggcgatgtc gagagcgtgc
      901 gtgccggaaa tcaccagcgg cgaaatcgtc tccggcgcgt cgctgcgctg atagttgtcg
      961 atcgccacca gccgattcat aaacgcgcgc gaagtcacca gcgtcacatc gcccagcgcg
     1021 cctgtgcgaa ctttttcctt cgccgccagc caccgccgcc gaaaccgctg cgtatagccg
     1081 accaccgcat cgatgccaga cttctcgatc gcctccagca ccttcgccga ttcagcgatc
     1141 ttcgtcgcca gcggcttttc gatcatcagc gggatgccgc gctccacgcc gcacatcaca
     1201 ggatccacgt gcaaatgctc gtccgtggca atgatcgccg cgttcacttc cgggcggctt
     1261 agcagttcgc gatggtcgct cgttacgaaa tccgcgccga tttctttcgc aaccgcctcc
     1321 gcccgctcag gcctcttttc ggcaatgccg atccatccca ccgagccgtg ccgcgccgcc
     1381 accgctcccc gaaataatcc cacgcgcccc gcgcccacaa tcgccagccc gatctgcttt
     1441 gaatttcttt tcatttctta ttgaccaatc ctccatcttc gcgcgagcgg tgtcgaatag
     1501 gctagtgagc gggaagtacc taaagtcgac tgtggttaga atcttgttgt ggagcgagat
     1561 tctttaaagt ccaaatttac aaggcagcct ctcttagcga agctttttca tgagtgccgc
     1621 gactgtcatg tcgctgtcct taggcccggt gtattggcga ctaatctcgg cgactatggc
     1681 atgaggcgtg tactcgcgga cttccgagaa ttgcgccttt cacaagacgg actgtgtgca
     1741 acttgttcgg caaagcaaaa gatagacgat tagggcagtg caggtctctg gcggacgcgc
     1801 atcgttgcgc taccatgaaa ccctaacgcg aatcgcatgc gcactcgcac caacgtccgc
     1861 tggtggatct gcttcttcct tttcctcacc tggcttgtgt cctacattga ccggagcctc
     1921 atgcccatgg ccttgccgtt cattggccag gaatttcatc tcagccccac agtcatggga
     1981 gcggtaatca gcgcgttctt cgtcggctac gccaccatgc aaattcccgg aggcatgctg
     2041 gcagatcgca ttggcccgcg caacagcatc acgctcggca tcgcgttctg gtcgggcttc
     2101 agcgtgctca ccggcctcgc cggaaatctc gccagcctgc tcgccgtgcg cgtcggcttc
     2161 gggctcgccg aaggactcca tcccgccgcc gcattcaaag cgctatccgc gtggttccct
     2221 gcgcacgaac gttcgcgtgc caacggactc gtcatgtcct ccaacgcgct cggccccatg
     2281 atcgcgcccg tgctgttcgc ctcgctcatg tccgccttcg gatggcgcgt tgcattttac
     2341 atcgtctcgg tgcccggact cctcgtcgcg gtaggagcat attggctctt gcgcgatcgc
     2401 cccgcccaac acccgcgcgt aacgccggaa gaactcgccg aaatcggcaa cgacgaagag
     2461 tcgcagccca agcccccatt ccgggagctg ctgaaatatc ccgtgctgtg gcggctcttc
     2521 gccatctaca tgttgtggga catcacgtgg tggggattcc aagcctggct cccttcttac
     2581 ctatttaaag tgcgcggatt ttcgctggcg cacaccggat tagtgacggc gctgccctat
     2641 gctgcaggat tcgtcggcgt cactctcgca ggttggattg ccgatcgcac gcgcaagcgt
     2701 gaagccgttc tcgccgtagt gttggtcggc atcgcgacgt tcatggtgct gacagccacg
     2761 gcccccacgg ccgtaaccgc cgtgattttt ctcacggccg ccggattctt tctgcccgcc
     2821 gtgcatggcc ccttctggtc cctgctgatg gaaatgctgc cttcgcgcgt gatgggctat
     2881 tcgtcgggat tcatcaacac cggcggccag atcgccggca tcctcgcgcc catcgtgatc
     2941 ggcgcgctca ttcagtggac cacgcactac tacgctggat tcatcttcat ggcgatttcc
     3001 gccaccattt cggcgttgct cgtcgcgacc ttgccgcgaa cggcgtcctc acaccctccg
     3061 aaaatcgccc ccgtagttcg cgaacgcgtc caaagtcgat agccagaatc gcggttcgcg
     3121 caggggacgg cggagcgccc gctccgcgcc gaggcgcccg cctcggctct tgtccagcgc
     3181 cgcacgaaac cagcggcccg ccgcctggcg gttcctaaac caccttgagc aataccgccg
     3241 tgatattgtc cggcccgccg tgctcctttg cgatagccac caggctccgg catttctctt
     3301 ccagcgtgcc gccatcggcc atcgtcgccg ccagattctc ttccggcacc acgccgtgca
     3361 gcccatcgct gcataacagc accagcatgc cgggcttcgg ctccacgcga tacgtctgca
     3421 cgcgcagttg atcggaaacg ccgatcgcca tggtcagcac gtgccgcatg ggatgcgtct
     3481 tcagcaccgt ttcctccagc cccaggcggc ggcccacttc attcacccac gattgatctt
     3541 cggtaatggt gtgcagcgcg ccgtcctgga acgaatacac gcggctatcg cccacgctcg
     3601 ccacaaacag ctcggtagtg ttgtcgagca ctgcaatcag cgtcgtgccc atgccgtcca
     3661 gttccggatc gtgctcggcg gccatcgaaa cgcgcgtgtt ggcttcggcg aacgcggtct
     3721 gcaaaacggc gggtccgcgc gatgccgaca tcgcgacgat ttccttcacc gtatccacag
     3781 ccatatgcga cgcgtgctcg cccgcctgcg cgccgcccat gccgtcggcc acaacgtata
     3841 aacccatggc cggcgccagc aggtagcagt cctcattgtt cggacgaaca cagcccgggt
     3901 ccgacacgcc aaatgactca atcatgaact tcttaaatta tatcgatgtc cacacttctg
     3961 ccgtacgatg ccgcggagcg cccagcgttt ttcagcattt gcgcgattcc gccaatgctc
     4021 gccccgctct cagcctgcag cggcgtttca gcgcgcgaaa cacatccgca gcacaccgcg
     4081 ttcctctgtc accggtgcgc c
//
LOCUS       DASZMC010000158         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_60615,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000158 DASZMC010000000
VERSION     DASZMC010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60615"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..321
                     /locus_tag="VG274_03115"
     CDS             91..321
                     /locus_tag="VG274_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWE05671.1"
                     /translation="MEFDRITQTPDVMGGKACIRGMRVTVGMLVGQIGAGRSIEELVA
                     DYPYLERDDILQALKYAAWLAHNSGIVTTERP"
     gene            complement(302..1153)
                     /locus_tag="VG274_03120"
     CDS             complement(302..1153)
                     /locus_tag="VG274_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE05672.1"
                     /translation="MNTPPPKKKKKATRSRSGTISDRSAIESLVGPLVALEHSGPLEQ
                     AQDIMWDAFDAPDRRQRVALAKKALKLSPLCADAYVVLAQETAENVTEALEFYRNGVE
                     AGEKALGKAAFRDNVGHFWGILETRPYMRARRGLAQSLWEGAAREEAVAHFQDLLRLN
                     PGDNQGIRYILLDCLLTMGRDDEAGKLLRRYKNDGAAAWQWSQALATFRREGDSPQSR
                     KALALAVRSNGHVSSYLLENRKLPRGLPDLISTGGEDEAIAYVANATPAWRAAPGALA
                     WVKDVLS"
     gene            1380..>1789
                     /locus_tag="VG274_03125"
     CDS             1380..>1789
                     /locus_tag="VG274_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWE05673.1"
                     /translation="MATLRINGEERTFDAPPDMPLLWLLRDVLGLTGTKFGCGIAQCG
                     ACTVHMDGNAVRSCLLPIGSIGDRAITTIEAVGETPVGAKVQKAWLDVEVVQCGYCQP
                     GQIMSAAALLAATPHPDDSDIDAAMAGNICRCGT"
BASE COUNT          319 a          561 c          566 g          343 t
ORIGIN      
        1 gctacatcga tactcacgtt tcgaccgtag tcacggcagt tcggctgacc taaactggat
       61 tgcgatttgg cgcacaaccg ggagcagaac atggagtttg atcgcatcac gcaaaccccc
      121 gatgtcatgg gcggcaaggc ctgcattcgc ggcatgcgcg tgaccgtcgg catgctcgta
      181 gggcagatcg gcgccggccg cagcatcgag gagctcgtcg ccgactaccc gtatctggaa
      241 cgcgacgaca ttctgcaggc gctcaaatat gcagcctggc tcgcccataa ttcggggata
      301 gtcacgacag aacgtcctta acccacgcga gcgcgccggg cgctgcacgc cacgcaggcg
      361 tcgcattggc gacatacgcg attgcctcat cttctccgcc cgtgctgatg aggtcgggca
      421 gtccgcgcgg gagcttcctg ttttcgagca gataagatga gacatggccg ttgctgcgga
      481 ccgccagggc caatgccttt cgcgattgcg gactgtcgcc ctcgcgccgg aacgttgcca
      541 gggcttgcga ccactgccac gccgcagcgc cgtcgttttt gtagcgccgc agcagctttc
      601 ccgcctcgtc gtcgcggccc atggtcagga gacaatcgag caggatgtag cgaatgccct
      661 gattgtcacc cgggttgagc cgaagcaagt cctggaaatg ggcgacggct tcctcgcgcg
      721 cggcgccctc ccacagcgat tgagcgagac cccggcgggc gcgcatgtaa ggacgcgttt
      781 cgagaatgcc ccagaaatgc cccacattgt cgcggaacgc ggccttgccc agcgccttct
      841 ctccggcttc cacgccgttg cggtagaatt ctagcgcctc cgtcacattc tccgccgtct
      901 cctgcgccag cacgacgtag gcgtcggcgc acaatgggga gagtttcagc gcttttttcg
      961 cgagcgccac gcgctgccgg cgatctggcg catcgaaggc atcccacatg atgtcctggg
     1021 cttgttcgag cgggccggaa tgctccagcg cgaccaacgg ccccacgagc gattcaatgg
     1081 cgctgcggtc cgagatagtc cccgaacgcg agcgcgtggc ctttttcttc ttcttgggcg
     1141 gcggcgtgtt catgattccg cgatgtccat gcgaatggag cgggatcaca gcatattcgc
     1201 cgggccggcg ccatcgatcg ggacccggtg gccgtgtcaa tcgagctttc caacatccgg
     1261 ctgggttagc tggtgcggat tcggctggcg ctcgatcagg aacagtgcct atgaatccgt
     1321 aacaggatag gttcggcggg caggaggctc gttccgcagg agcaaaaggg gggaccaata
     1381 tggcgacact tcgtatcaat ggtgaggagc ggacgttcga tgcgccgccg gacatgccgc
     1441 tgctttggtt gctacgcgat gttcttggac tgaccggcac gaaattcggt tgcggcattg
     1501 cgcaatgcgg cgcgtgtacg gtgcacatgg acggcaacgc ggtgcgctcc tgccttcttc
     1561 ccataggctc gatcggcgac cgcgcgatca ccaccatcga agctgtcggc gaaacgcccg
     1621 tcggcgccaa ggtgcagaag gcgtggctcg atgtggaagt cgtgcaatgc ggctattgcc
     1681 agcccgggca gatcatgtcg gcggctgccc tgctcgcggc gacgccccat cccgatgatt
     1741 ccgatattga cgctgcgatg gcaggcaaca tctgccgctg cggcaccta
//
LOCUS       DASZMC010000159         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1978351,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000159 DASZMC010000000
VERSION     DASZMC010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978351"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VG274_03130"
     CDS             complement(<1..569)
                     /locus_tag="VG274_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05674.1"
                     /translation="MKKLAVLFASAATAMIAAVAVAQTTPPSPGAGAGSDLPPGLIRQ
                     GGIVMMQPLSDSGQRPSGATIFGGERRTALVGFLSASDHQIYSRSVDAAIRGDWAAAR
                     GLADQGRDPAARQIIEWAYLLDKNSGATFEQIGQFLRTNPAWPGRNKLLARAEEAILP
                     TMDPHAVIAWFGDRAPQSGPGKIRLGEAL"
     gene            743..1615
                     /gene="dapA"
                     /locus_tag="VG274_03135"
     CDS             743..1615
                     /gene="dapA"
                     /locus_tag="VG274_03135"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWE05675.1"
                     /translation="MPALITPFCDGCVDESALRRLVAWHIAEGSHGLVPAGTTGESAT
                     LSHEEHQRVVEICVEEASGRIPVIAGAGSNSTNEAIALTRHARAVGADAVLSVAPYYN
                     KPSQEGLYRHFAAIAEAVDIPIVVYNIPGRSVVEISLETMERLSRITNIVGVKDATAN
                     LARPSRERNACGESFRLLSGEDGTALGYMAHGGHGCISVTANVGPRLCSAFQEACLRG
                     DFGAALAMQDRLMPLHDALFCEPSPAPVKYAVSLLGFCSDAVRLPIVEASDTARARIR
                     KAMEAAGIPCRADA"
     gene            1627..2103
                     /gene="smpB"
                     /locus_tag="VG274_03140"
     CDS             1627..2103
                     /gene="smpB"
                     /locus_tag="VG274_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWE05676.1"
                     /translation="MARKKEEPERRVVADNRKARHAYAIESTLETGLALMGSEVKALR
                     SGRATIAESYAQAKDGEIFLINSYIPEYAMANRFNHEPRRPRKVLVHKKEGRRLAAAV
                     QREGMTLIPLRVYFNVKGVAKLELGVARGKKLHDKRETEKARDWARDKARLLRAKG"
     gene            complement(2131..2778)
                     /locus_tag="VG274_03145"
     CDS             complement(2131..2778)
                     /locus_tag="VG274_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWE05677.1"
                     /translation="MSTGESACEPPRDCPLCPRLAKFRNANRAAHAGYFNDPVPTFGD
                     SSGRLLIVGLAPGLHGANRTGRPFTGDHAGQLLYSSLLRHGLARGAYAARADDGLELV
                     DCAITNAVRCVPPDNKPLPAEIRTCRQFLLAQLAAMPKLRAALTLGRIAHESVLRALD
                     IPLREHPFAHGGQYASGKLILVASYHCSRYNTNTGVLTPKMFDDTLAAAKAAAVR"
     gene            complement(2805..3392)
                     /locus_tag="VG274_03150"
     CDS             complement(2805..3392)
                     /locus_tag="VG274_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWE05678.1"
                     /translation="MLFYPQEKLALFIDGANLYGAAKGLAFDIDYKRLLELFARKGVL
                     VRAFYYTAVAEDQEFSPLRPLVDWLDYNGFSVVTKPLKEFTDSQGRRRVKGNMDIELA
                     IDVMELAETVDHIVIFSGDGDFRRLVEAAQKKGRRVSVVSTIRSQPPMIADELRRQAD
                     NFIELEELKPLIMREGGPRAGQSVQQQVAQFAQTG"
     gene            3496..3984
                     /gene="folK"
                     /locus_tag="VG274_03155"
     CDS             3496..3984
                     /gene="folK"
                     /locus_tag="VG274_03155"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWE05679.1"
                     /translation="MILIALGANLPSPAGPPRATLEAALHELRRRNISVPARSNFYKS
                     RAWPDPREPCFINAVAEIEAKQTPRELLVLMREIEIMFGRTITRRNAPRTLDLDLLDY
                     NGLVEAGTPILPHPRLQERAFVLVPLSDVAPDWRHPVSGLGVRTLIAALPSEATALTK
                     LP"
     gene            4004..>4455
                     /locus_tag="VG274_03160"
     CDS             4004..>4455
                     /locus_tag="VG274_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05680.1"
                     /translation="MDWHALAPYLVPLLVAAIILRRVMRVQKARTIRVERLWIFPALL
                     LAATVLSLGRENFPGIGILALFLASAGLGAAIGWFRVHTLEFSLDAESGAVSARATRL
                     GALLIFGLIALRYGAELALKKLGLNAGANLVHATDAMLVFSTSMLVAR"
BASE COUNT          813 a         1364 c         1421 g          857 t
ORIGIN      
        1 atcaatgctt caccgagacg aatcttgcct ggcccgctct ggggcgcgcg atcgccgaac
       61 caggcgatca ccgcatgggg atccattgtg ggaagaattg cttcttcggc gcgcgccagc
      121 aatttgttgc ggccaggcca cgcgggattg gtcctgagga actggccaat ttgttcgaac
      181 gtcgcgccgc tgttcttgtc cagcaggtaa gcccattcaa tgatttgacg agccgccggg
      241 tcgcggccct gatccgcgag accgcgagcg gccgcccagt cgccccgtat cgccgcgtcg
      301 acggagcggc tgtaaatctg gtgatcggag gcgctcagaa agccgaccag ggccgttctg
      361 cgctcgccgc caaagatggt cgcgcccgaa ggtctctgac ccgaatccga gagaggttgc
      421 atcatcacga tgccgccctg ccggatcaaa cctggcggca ggtcggagcc tgcgccagcg
      481 ccgggtgagg gcggagtcgt ttgcgctacc gcaacggccg caatcatcgc ggtcgccgcc
      541 gacgcgaaca ggacagccaa cttcttcatc gtgcgcgcga agccgccgga ttttccgtga
      601 atcggaaccc tatgaggata gtggggcgtc ccccgatttg ccagcaggcc cgccttgccg
      661 ccgcggcgga gggggcgtta atttggcgca acattccgtg ggattcgtaa tgcgtgatgc
      721 gcgcgaacgc ctgagcggct cgatgcctgc tctgattacc ccgttttgcg acggttgcgt
      781 ggacgaatct gcacttcgcc gcctcgtcgc ctggcacatc gccgagggat cgcacggact
      841 tgtgccggcc ggcacgaccg gcgagtccgc gactttgagc cacgaggagc accaacgcgt
      901 cgtggagata tgcgtcgagg aggcgagcgg tcggattccc gtgattgccg gagccggctc
      961 caattcgacc aacgaagcga tcgctctgac gcgccacgcc cgggccgtgg gcgcggacgc
     1021 cgtgctgtcc gtcgcgccct actacaacaa gccgtcgcag gaaggtcttt accgccattt
     1081 tgccgccatt gccgaagcgg tcgatatacc catcgtcgtc tacaacattc cgggtcgcag
     1141 cgtcgtcgag atctctctgg agaccatgga gcgcctttcc cgaatcacaa atattgtcgg
     1201 cgtgaaggac gcgacagcca atcttgcccg cccaagccgc gagcgcaacg cgtgcggaga
     1261 atcgtttcgt ctgctgtctg gcgaagatgg gaccgccctg ggctacatgg cccatggagg
     1321 acatggctgc atatcggtta ccgcaaatgt tggaccgagg ctctgttccg cttttcagga
     1381 ggcctgcctg cgtggtgatt tcggggctgc gctcgcgatg caagatcggt tgatgcccct
     1441 gcatgatgcc ctgttctgcg agccgagccc cgcgcccgtg aaatatgccg tttctcttct
     1501 tggcttctgc agcgatgcgg tccgcctgcc gatcgtagag gcaagcgaca ccgcacgcgc
     1561 acgtatccgc aaggcaatgg aagccgcggg catcccttgc cgcgccgacg cgtgacgagc
     1621 tgaccggtgg cgcgcaagaa ggaagaaccc gagcggcgcg tcgtcgccga caatcgaaag
     1681 gcccgtcacg cctatgcgat cgagtccact ctggagacgg ggctggcgct gatgggttcg
     1741 gaggtgaagg cgcttcggag cggaagggcc acgatcgcgg aatcctatgc gcaggcgaag
     1801 gacggcgaga tattcctgat caattcctac attccggaat acgcgatggc gaatcgcttc
     1861 aatcatgagc cgcgccgccc gcgcaaagtt ctggtgcaca agaaggaggg gcgcagactc
     1921 gccgccgcgg tgcagcgcga gggcatgacg ctcattcccc tgcgggtgta tttcaacgtc
     1981 aagggcgtag caaagctgga gcttggagtt gcacgcggaa agaagctcca tgacaaacgc
     2041 gagaccgaaa aggcgcgaga ctgggcgcgc gacaaggccc ggctgctgcg ggcgaagggt
     2101 tagccagggg ccgcccgcct gtcgaacctc ctatcgaacg gccgcggctt tcgcagcggc
     2161 aagcgtgtcg tcgaacattt tcggcgtgag aactcccgtg ttcgtgttgt aacgcgagca
     2221 atggtagctc gcgacgagga tcagcttccc gctcgcgtac tggcctccat gtgcgaaggg
     2281 atgttcgcgc aatggaatgt cgagcgcccg gagcacgctc tcatgtgcaa tgcgtccgag
     2341 cgtcagcgcc gctcgcagtt tgggcatcgc ggcgagctgc gcgagcagga attgccggca
     2401 ggttctgatt tccgcgggca gcggcttgtt gtcgggaggt acgcaccgca ccgcattggt
     2461 gatcgcgcaa tcgacaagct caaggccatc gtccgcgcgc gcggcgtatg cgccgcgcgc
     2521 aagcccgtgc cggagcaggg acgagtaaag aagctggcct gcgtggtccc cggtgaacgg
     2581 ccggcccgtg cgattggcgc cgtgaaggcc gggagccagg ccgacaatca gcagccgccc
     2641 actcgaatcg ccgaacgtcg gtaccggatc gttgaaatag cctgcatgcg ccgcccggtt
     2701 ggcattgcga aattttgcca ggcgcgggca aagagggcag tcgcgcggcg gctcgcaggc
     2761 gctttcaccc gtcgacatga catcccggcg cgtgcagtag cggttcagcc ggtctgcgcg
     2821 aactgggcga cctgttgttg aaccgattgg ccggcgcgcg ggcctccctc gcgcatgatc
     2881 aacggcttca gctcctcgag ctcgatgaaa ttatcggcct ggcgccgcaa ctcgtcagcg
     2941 atcatgggcg gctgcgaccg gatcgtagac acaacgctga cgcggcgccc ttttttttgt
     3001 gcggcttcga cgaggcggcg aaaatcgccg tctccggaaa agatcacgat gtgatccacc
     3061 gtctcggcca attccatcac gtcgatggcg agctcgatat ccatgttgcc cttcacgcgc
     3121 cgccggccct ggctgtccgt gaattccttg agcggtttgg taacgaccga gaatccattg
     3181 tagtcgagcc agtcgacgag cgggcgcaac ggcgagaatt cctgatcttc cgcgacggcg
     3241 gtgtaataga aggcgcgcac gagcacgccc ttgcgggcaa acagctcaag caaacgcttg
     3301 tagtcgatgt caaaggcgag accttttgcg gcgccgtaga gattggcgcc gtcgataaat
     3361 aatgcgagtt tttcctgggg gtagaacaac atcccaattt ctcctgttga caatgagcca
     3421 agtcgcaatc aatcagcccc cggaaccgtt ctatgatatt cggcagccgg tgcaagtgct
     3481 gaacatcgaa ttcaaatgat tttgatagcg cttggcgcca atctcccatc gcctgccggt
     3541 cctccccgcg caactctgga ggcggcgttg catgaattgc ggcgcaggaa catttccgtt
     3601 cccgcgaggt cgaattttta caaaagccgc gcttggccgg atcctcgcga accctgcttc
     3661 atcaatgccg tcgcggagat agaggccaaa caaaccccga gggaactcct ggttctgatg
     3721 cgggagatcg aaatcatgtt cggccggacg atcacgcggc gaaacgcacc gcgaacgctt
     3781 gacctcgatc ttctggacta caatgggttg gtcgaggcgg gcacgccgat attgccgcat
     3841 ccccgtcttc aggagcgcgc cttcgttctc gtgccattat cggatgtcgc accggactgg
     3901 cgccatcccg tctcggggct cggcgtgagg acgctcattg ctgccttgcc cagcgaagca
     3961 acggcgctta cgaaactgcc ctgacttgcc ggttggatat gcgatggatt ggcatgcgct
     4021 cgcgccctac ctggtgccat tgctggtggc ggcaatcatc ttgcggcgtg tcatgcgcgt
     4081 tcaaaaggcg cgcaccattc gcgtcgaacg gctgtggatt tttccggcgc ttcttttggc
     4141 ggcgaccgtg ttgtcgctcg gacgggaaaa ttttcccggc attgggatcc tcgcgctgtt
     4201 cctggcctcg gctggtttgg gcgctgcgat cggctggttc cgggtccaca cgctcgaatt
     4261 cagtctggac gcggagagcg gcgctgtttc cgcgcgagcc acgcgcctcg gtgcgctgct
     4321 gatattcggt ctgatcgcat tgcggtacgg agccgagctt gcactcaaga agctcggatt
     4381 gaacgccggc gcgaacctcg ttcacgcgac ggatgccatg ctcgtctttt ccacctcgat
     4441 gcttgtggcg cgaag
//
LOCUS       DASZMC010000160         4976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3912570,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000160 DASZMC010000000
VERSION     DASZMC010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3912570"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..918
                     /gene="mutM"
                     /locus_tag="VG274_03165"
     CDS             43..918
                     /gene="mutM"
                     /locus_tag="VG274_03165"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWE05681.1"
                     /translation="MPELPEIETVRMGLAPALEHRRLRSVVTRRGDLRVPFPPRFAER
                     LAGREVKRLWRRAKYLLAELDDGETLVIHLGMSGRVAVYAESQEHRLGHYVYEPPPSR
                     AGQGKHDHVVIETDAPARIVFTDPRRFGLMLLIESAVLDDHPLFDGLGIEPLSPQFTA
                     ARLKRILEGRRAPIKAALLDQRTIAGLGNIYVCEALFRARIAPRRQSSTIAGGRARAL
                     AAAIRSVLRAAIKAGGSSLRDYAKTDGALGEFQHHFAVYDREGAPCPRARCKGKIKRI
                     VQSGRSTFYCPACQR"
     gene            complement(1086..1553)
                     /locus_tag="VG274_03170"
     CDS             complement(1086..1553)
                     /locus_tag="VG274_03170"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWE05682.1"
                     /translation="MNDVLDGGCACGAVRYRLNSAPMFVNCCHCRDCQRQTGSGFVIN
                     ALIETDRITLLASQPEPVPVPTQSGRPHDVYRCSNCRIAVWSDYGRRPGLRFVRVGTL
                     DRPDALTPDAHIFVRSKLPWVGLPDNVPAFDVYYDMKSLWPPESLERRRAISG"
     gene            1618..2043
                     /gene="arfB"
                     /locus_tag="VG274_03175"
     CDS             1618..2043
                     /gene="arfB"
                     /locus_tag="VG274_03175"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWE05683.1"
                     /translation="MAKIAITQQFSIDESEIEESFILAGGPGGQNVNKVASAVQLRFN
                     VRRSASLPENVRERLIRIAGGRITKSGELLLLGRRYRSQERNRADVRARLAALLRDAA
                     RPVEPRRKTKPPPASKAHRIENKLARGLVKRLRTRPHED"
     gene            2106..2882
                     /locus_tag="VG274_03180"
                     /pseudo
     CDS             2106..2882
                     /locus_tag="VG274_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
     gene            2958..3224
                     /gene="rpsT"
                     /locus_tag="VG274_03185"
     CDS             2958..3224
                     /gene="rpsT"
                     /locus_tag="VG274_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWE05684.1"
                     /translation="MPNIDSAKKRVRQIAKRTAVNHARKSRIRSITRKVEEAIATGDK
                     SAALAALKAAEPEIMRGASKGVLHRNTGSRKVSRLAKRTAKLPG"
     gene            3663..4112
                     /locus_tag="VG274_03190"
     CDS             3663..4112
                     /locus_tag="VG274_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWE05685.1"
                     /translation="MAQVDSAAGYAGDLLPDAAWALLERQPMAQLVDVRTTAEWNFVG
                     QPDLSSLGRTAHLVEWQNFPSMALNPEFVASAAHHLEASGAGKETPLVLLCRSGARSR
                     AAAIALTKAGYAQAFNIAGGFEGDANAAGHRGVTNGWKAAGLPWRQG"
     gene            4252..>4976
                     /locus_tag="VG274_03195"
     CDS             4252..>4976
                     /locus_tag="VG274_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HWE05686.1"
                     /translation="MTQALTSTPDWLAIWSAARGRLRRDLGVPVFEAWIGKLSLLDFH
                     RGDLKFGAPRPFVRNWVANNYVGRIEKALRAEGGIPTSITIVLAASDAPAIGGGLMSE
                     ETSSPAPLASVSVLSPHHAHAGRREDGLRASGGQALFFRAPDPALSFASFVEGQANAL
                     ALRASRAFADGNADDVSLLYIHGGFGFGKTHLLNATALESRRRGWRTLLLGSEDFMRQ
                     FLGALNRKETLGFKEELRAADMLL"
BASE COUNT         1019 a         1536 c         1549 g          872 t
ORIGIN      
        1 aattgaaata tcatctctcc ggcctccgat cgctgacatc ccatgcccga gcttcctgaa
       61 atcgaaacgg tgcgaatggg ccttgcgccc gcgcttgaac atcgccggct gagaagtgtg
      121 gtgacgcgcc gcggggatct ccgggttccc tttccgcccc gatttgccga acgtctcgca
      181 ggccgcgaag tcaaacgcct gtggcgacgc gccaaatatc tcctcgccga actcgatgac
      241 ggcgaaaccc ttgtgattca tcttggcatg tccgggcgtg tcgcggtcta tgcggagagc
      301 caggaacaca gactcggaca ttacgtatat gagccgccgc cctcccgcgc cggtcagggc
      361 aaacacgacc acgtggtcat tgaaaccgac gcgcctgctc gcatcgtgtt taccgatccc
      421 cgcagattcg gtctcatgct tctcatcgaa tcggcggttc tcgatgacca tccattattc
      481 gatggactcg gtatcgagcc cctgtccccg caatttacgg cggcccgtct caagcgcata
      541 ttggagggcc gtcgagctcc gatcaaagcc gctcttctcg accagcgcac gattgcgggg
      601 ctcggcaaca tctatgtctg cgaggccctg tttcgcgcgc gcatcgcgcc gcggcggcaa
      661 tcgtcgacca tcgccggcgg tcgcgcgcgc gcgcttgcgg cggcaatcag gagcgtattg
      721 cgcgcggcga tcaaggccgg cggatcaagc ctgcgcgatt acgcaaagac cgacggtgcg
      781 ctcggcgaat tccagcacca tttcgccgtc tacgaccgcg aaggggcgcc ctgcccgcgc
      841 gcccggtgca agggcaaaat caagcgcatc gtccagagcg gccgctccac cttctattgc
      901 cccgcatgcc agcgatagaa caagaatggg cacgcggagc cgcgggggaa cgcggaggta
      961 gcagattcac cacaaagagc accaaggtca cgatggaagg atcgcgcgcc gaaggcgcgc
     1021 aaaaacactt tgtgcgcttt gtgctctttg tggcgaacaa ttcccctgcg cgtcttccgc
     1081 acgggtcatc ccgagattgc gcggcggcgt tcgaggcttt ccggcggcca gagggacttc
     1141 atgtcgtaat agacgtcgaa ggcgggaaca ttgtccggca atccgaccca gggcagcttc
     1201 gaacggacga agatatgcgc gtcgggcgtc agcgcatcgg gccggtcgag cgtgccgaca
     1261 cggacgaaac gaaggcccgg ccggcggccg tagtcgctcc acacggcgat ccggcagttc
     1321 gaacaacgat acacgtcgtg cggtcgtccg ctctgcgtcg gcaccggcac gggttccggt
     1381 tgcgacgcga gaagcgtaat ccggtcggtc tcgatcagcg cgttgatgac gaaaccgctg
     1441 cctgtctgtc gctgacagtc gcggcaatgg caacaattca caaacatcgg tgcggagttg
     1501 agccggtagc gcaccgcgcc gcaggcgcaa ccgccgtcaa gaacgtcgtt catcatcctc
     1561 tcccgcaaga ccgaccgcca tttgattgca caaggaaagc catgcgaaaa gtgccgaatg
     1621 gcgaagatcg cgattacgca gcaattctcg atcgacgaga gcgaaatcga ggaaagcttc
     1681 attctggcgg gcggacccgg cggccagaat gtgaacaagg tcgcaagtgc ggtgcagctg
     1741 cgcttcaacg tgcggcgctc cgcgtccctg ccggagaacg tgcgcgagag actgatccgg
     1801 attgcgggcg gccggattac gaagtcaggc gaacttctgc ttctcgggcg gcgctatcgc
     1861 agccaggaac gcaaccgcgc cgatgttcgc gcgcggctcg ccgccctgct gcgggacgcg
     1921 gcccggcccg tcgagccgcg gcgcaagacg aagcctccgc cggcgagcaa agcgcaccgc
     1981 atcgagaaca agctggctcg cggcctcgtc aagcgattgc gcacgcgccc ccacgaagat
     2041 tgattggctc ttgcgttggc gcggcgcgcg cgcgagggat gcgcgagcag aagcggagtc
     2101 cgagcatgga atacgaaaac attctcctca aaactgaagg cagggtggcg atcgtcactc
     2161 tcaatcgccc caaggcgctc aacgcgctga attccgcgct tctgggcgag cttgtgcatg
     2221 cgctcgaaac catcgacgcc aacgacgggc tgcacgcgat cgttcttacc ggttcggacc
     2281 gcgcgttcgc cgccggggcc gacatcaagg aaatggcgcc gcaatcctac atggacatgt
     2341 tcaaggcgaa ttttttcgcg gacgcggcag atagcatcgc tgccattcgc aagccgatcg
     2401 tcgcggccgt ggccggatac gcgctgggcg ggggctgcga actcgcgatg ctgtgcgatt
     2461 tcatcattgc cgcggacaac gcgaaattcg gtcaaccgga gatcaatctg ggcgtcatgc
     2521 ccggcatcgg cggcacgcag cgcctgacgc gcttcgtcgg ctagtccaag gcgatggaca
     2581 tgtgcctgac agggcgcaac atggatgcgc aggaggccga gcgcgcaggg ctggtcagcc
     2641 gcgtcgtccc cctggccgag ctgctggcgg aagcgacgaa cgccgcgcac aagatcgcgg
     2701 agcaatcgct tcccatcgtg atgatgacca aggagacgat caatcgcgcc tatgagacga
     2761 cgcttgccga agggatccgt tttgagcggc gcgttttcca ctccatgttt gcgaccgacg
     2821 accagaagga aggcatggcg gccttcgcgg aaaggcgcaa gcccgcgttt cggaaccggt
     2881 aagcccgcac cgcgcattga ccgggccgcg gggcgcgggc taagaaccgc tccctcccag
     2941 tgagcgagag aacgcctatg cccaatatcg actctgccaa gaaacgggtg cgccagatcg
     3001 ccaagagaac agcggtcaat cacgcccgca agagccgaat ccgaagcatc acccgcaagg
     3061 tcgaggaagc cattgcaacg ggcgacaaat cggcagcgct tgctgcgctg aaagctgccg
     3121 aacctgaaat catgcgcgga gcctcgaagg gcgttctcca caggaacacg gggagccgaa
     3181 aagtgtcgcg cctcgccaaa cggacggcaa aactgcccgg ctaagcgcct gaaaacttgt
     3241 gttaaatttc tgtgacgccc caaaacgtct ttcagaacca cggttttgtc gctccgtaag
     3301 cggagttttc attttgggac cggctgaaaa aaaaacttca cgtgatgcgc tgcgactaac
     3361 cgtctgaaaa cgcccaattt ccaattttca gtatacgtcc gcgtgactta gcaaccgtca
     3421 cacaacccac atattcccga ttcgttcgcg ttgcaactcg ttaacgacgt gctatcgttt
     3481 gttggctggc gcgcagttac gccagaaaac agcaaataaa ataccaatct taaaaacgcg
     3541 ccgaagaaac aaaaagccgg aagggctagt ttcggagtcg cgaggggcag tctagtctca
     3601 aagttttact ttgatgtcgg tgccgcatgg gcgcttgggg gtgggctttg cctggagcaa
     3661 aaatggcgca ggtcgactca gcagccgggt atgccggcga tcttctcccc gatgcggcct
     3721 gggcgctgct cgagcgccag cccatggcac agctggtgga cgtgcgcacg acggccgaat
     3781 ggaacttcgt gggccagcca gacctctcca gcctgggccg aaccgcccat ctcgtcgagt
     3841 ggcagaattt cccgagcatg gccctcaacc ccgaattcgt cgcgtcggcg gcccaccatc
     3901 tggaagcaag cggcgccggg aaggagacgc ccctggtgct cctctgccgc tccggcgccc
     3961 gctctcgtgc cgcggcgatc gcactcacga aagcgggtta tgcccaggcg ttcaatatcg
     4021 caggcggctt cgaaggcgac gcgaatgcgg caggacaccg cggggtgaca aatgggtgga
     4081 aagcggcagg cctgccatgg cggcaggggt agtaaagcaa aggagcaatg gcggcaaaga
     4141 aaaatccgat cgtggaacga gatcaagcaa gaatagatgt gatccaataa tcccagagaa
     4201 gggggaccgc gagacttact tctccggtcg tagcataggg caaatgggac aatgacgcag
     4261 gctctgacaa gcactcctga ttggctcgct atctggtctg cagcgcgtgg caggcttcgc
     4321 cgcgatcttg gcgtgccggt attcgaggcg tggatcggca aattgagcct tctcgatttt
     4381 cacaggggcg atctcaaatt cggggccccg cggccgttcg tacgcaattg ggtggccaac
     4441 aattatgtcg gccggatcga aaaggcgctg cgggccgaag gggggattcc gacatccatc
     4501 accatcgttc tcgctgcgtc cgatgcgcca gccatcggcg gcggattgat gagcgaggaa
     4561 acgtcgtcgc ccgcaccgct ggcctcggtc tctgtgctgt ccccgcatca cgcacatgcc
     4621 ggccggcgcg aggacggtct gcgcgccagt ggcggccaag cgctcttttt ccgcgcgccg
     4681 gatcccgccc tgtccttcgc cagtttcgtc gagggtcaag caaacgcgtt ggcgctgcga
     4741 gcctcgcgcg cctttgccga tggcaacgct gatgacgttt cgcttttgta cattcatggc
     4801 ggcttcggct tcggcaagac gcatttgctg aatgcgacgg cgctcgaatc gaggcgcaga
     4861 ggttggcgta cgctcctgct cggctccgag gatttcatgc gccagttcct gggcgccctg
     4921 aaccgcaagg agacgctggg cttcaaagag gagctgcgag ccgccgacat gttgct
//
LOCUS       DASZMC010000161         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_882160,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000161 DASZMC010000000
VERSION     DASZMC010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882160"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1522
                     /gene="der"
                     /locus_tag="VG274_03200"
     CDS             155..1522
                     /gene="der"
                     /locus_tag="VG274_03200"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWE05687.1"
                     /translation="MPLKLAIVGRPNVGKSTLFNRLAGKKLALVHDTPGVTRDRREAD
                     VAQGGLSLKLIDTAGFEEGADGSLASRMTAQTMAAISDADVCLFVIDARDGVTAGDEI
                     IAETLRRSGKPVILAANKSEGRGHTEAIVEAYRLGFGEALALSAEHNLGTDDLLEALA
                     EFDEESDSDPAQDTDEPERELEEDRPLRLAIVGRPNVGKSSLFNHILGDERSLTGPEP
                     GLTRDSITAPWTAENGRAVLLHDTAGLRKSARAAGHTLEQLSIQSTLDAVRFADCCIV
                     VIDATQPFEKQDLTIADLIEKEGRAVVFAVNKWDLVEDRAGAIGKLRRQLDESLPQLT
                     GSPLIAISARSGEGVNRLLPAVLEADRAWNLHIATSALNRFLESALARHAPPAVKGRR
                     VRIRYMTQPKVRPPTFALFGNQLDALPEAYQRYLANGLRDAFHLAGTPIRLRMRGGKN
                     PFAND"
BASE COUNT          337 a          534 c          499 g          301 t
ORIGIN      
        1 acggctttgc ggaatccatt tccccctata ccggcaagct tctcgggcga atggaaatac
       61 cggcggccgc ctttatcgcc cccgtcgtcg ccaatgatac gctctacctc tggaccaacg
      121 atgcgcagct ggttgcgctg cgctgacgcg tgtcatgcca ctcaaacttg cgatcgttgg
      181 acgccccaat gttgggaagt ccacactgtt caaccggctc gccggcaaga agctcgcgct
      241 tgtgcacgat acacccggcg tcacgcgaga ccggcgtgaa gcggatgtcg cacaaggcgg
      301 actttcccta aagctgatcg acaccgcagg ctttgaggaa ggcgccgatg gcagccttgc
      361 ctcgcgcatg acggcacaga ccatggcggc gatctccgac gcggatgtgt gcctgtttgt
      421 gatcgatgcg cgggatggcg tgacggctgg cgacgagatc atcgccgaga cgctacgccg
      481 ttccggcaag cctgtgattc ttgcggccaa caagagcgaa ggacgcggcc acactgaagc
      541 gattgtggaa gcgtaccgtc tcggcttcgg cgaagcactc gcattgtcgg ccgagcacaa
      601 ccttggcacc gacgaccttc ttgaggcttt ggctgaattc gacgaggaat cggattcgga
      661 tccagcgcaa gacaccgacg aacccgaacg ggagttggaa gaagacaggc ctcttcggct
      721 cgccatcgtc ggccgcccga acgtcggcaa gtcgagcttg ttcaatcaca tcctgggaga
      781 cgagcgctca cttaccggtc cagagccagg cctgacgcgc gactccatca ccgcgccgtg
      841 gacagcggag aatgggcgcg cggttctgct gcacgacacg gcaggtctgc gcaaaagcgc
      901 gcgcgcggct ggccatacgc tggagcagct atcgatccag agcacgcttg acgccgtgcg
      961 ctttgccgat tgctgcattg tcgtgatcga cgcaacgcag cctttcgaaa aacaggattt
     1021 gaccatcgcg gacctcatcg aaaaagaagg ccgtgccgtc gtgttcgcgg tcaacaaatg
     1081 ggatctggtc gaagaccgcg cgggcgccat cggaaaactg cgccggcaac tcgacgagtc
     1141 cctgccgcag ttgacgggca gcccattgat cgcgatctcg gcacgctccg gtgaaggcgt
     1201 gaaccgtctg cttcccgccg tgctggaagc cgaccgtgcg tggaacctgc atatcgcaac
     1261 atcggcactg aaccgctttc tggagagcgc actggcgcgt cacgcaccgc ccgcagtcaa
     1321 aggccgccgc gtgcgcattc gctatatgac gcagcccaag gtgcggccac ctaccttcgc
     1381 gctattcgga aatcagctcg acgccctgcc cgaagcttat cagcgctatc tcgcaaatgg
     1441 cctgcgtgat gcgttccatc tcgcaggcac gccgatccgg cttcggatgc gcggcgggaa
     1501 aaatcctttc gccaacgact agcgcttgaa cacgacgatc tcgccgttct tttcattcga
     1561 tggcaataga aaatccacaa tcagcggcac cacaacttcg ggcggcgcga gtgactccgg
     1621 attttcaccc ggcattgcac gcgcgcgcat cgccgtgcgc aacgggccgg g
//
LOCUS       DASZMC010000162         6634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4456668,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000162 DASZMC010000000
VERSION     DASZMC010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4456668"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VG274_03205"
     CDS             complement(<1..274)
                     /locus_tag="VG274_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWE05688.1"
                     /translation="MSLNKGFHSLRIAEVRRETADAVSIRFDVPAELREAFAFQAGQH
                     LTLKAEIGGEDVRRTYSVCVSPAENEIRIAIKQMPAGKFSAWANADL"
     gene            complement(281..775)
                     /gene="paaD"
                     /locus_tag="VG274_03210"
     CDS             complement(281..775)
                     /gene="paaD"
                     /locus_tag="VG274_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HWE05689.1"
                     /translation="MKAAARAQSVEALKDRVFELLSHVPDPEIPCVSVVDLGIVRDVR
                     EGTLVITPTYSGCPATLAIASAIRAALDRAGLSRLRIETVLSPAWTTDWISDEGREKL
                     HAYGIAPPPHGAARYSLRDEQAACPRCGSLHTEEISRFGSTPCKSLNRCLACGEPFDR
                     FKCH"
     gene            complement(772..1521)
                     /gene="paaC"
                     /locus_tag="VG274_03215"
     CDS             complement(772..1521)
                     /gene="paaC"
                     /locus_tag="VG274_03215"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HWE05690.1"
                     /translation="MKPLVEYALRLGDDGLVLGQQLSEWCGHSPMLEIDLALANIGLD
                     LIGQSSHFLRLAGEAEGEGRDADVLAFHRDVLDFRNALLVEQPNGDFAQTVARQFLFS
                     NWQELLMRELVRSQDETLSAIAGKAVKEVVYHARFANEWMVRLGDGTEESRKRIIAGL
                     EWMWRFTDELFAVDATMEELIVRGVAVDPRPLRAEWDARIAQTLAEATLEAPKPRRAA
                     SGGRSGHHSEHLGHLLTEMQFLPRAYPGAVW"
     gene            complement(1521..1805)
                     /gene="paaB"
                     /locus_tag="VG274_03220"
     CDS             complement(1521..1805)
                     /gene="paaB"
                     /locus_tag="VG274_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HWE05691.1"
                     /translation="MNKEWPLWEVFVRARNGLAHKHAGSVHAADARMAIEAARDTYTR
                     RMEGVSLWVVPSASIVASDPGEAGSLFEPAADKVYRHPTFYSIPENIKNI"
     gene            complement(1812..2759)
                     /gene="paaA"
                     /locus_tag="VG274_03225"
     CDS             complement(1812..2759)
                     /gene="paaA"
                     /locus_tag="VG274_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HWE05692.1"
                     /translation="MANEDPVRLAAFEARVAADDFIEPKDWMPEAYRKTLIRQISQHA
                     HSEIVGMLPEGNWITRAPSLRRKAVLLAKVQDEGGHGLYLYAAAETLGTSRDEMIEAL
                     HSGRAKYSTIFNYPTLTWADIGAIGWLVDGAAIMNQVPLQRTSYGPYARAMVRICKEE
                     SFHQRQGYEITMTLAAGSPEQKRMAQDALDRWGWPSLMMFGPPDDNSPNSAQSIRWRI
                     KRETNDQLRQRFVDVTVPQAEAIGLRVPDPALKWNKARESYDFGELDWEEFYAVVRGE
                     GPVAKERMNARVMAWEDGAWVREAALAHSAKQNGRLAAE"
     gene            3005..3796
                     /gene="paaG"
                     /locus_tag="VG274_03230"
     CDS             3005..3796
                     /gene="paaG"
                     /locus_tag="VG274_03230"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HWE05693.1"
                     /translation="MAYDTILLDIAKGAARLTLNRPDRLNSFNVEMHREVAEALSRVE
                     ADPEIRALLITGAGRGFCAGQDLSDRAVAPGADGVDLGESLENRYNPLIRRLIGLPKP
                     VVCAVNGVAAGAGANIAFAADIVLAAKSAKFIQSFSNIGLIPDSGGTWILPRLAGQAR
                     ALGLALTGEPLGAEKAESWGLIWRVFDDDQLAGEAEKLVERLAVGPTRGFAAIKMAMR
                     KGWLAGLEEQLDLERDLQRDLGRSGDYREGVAAFMEKRRPNFSGQ"
     gene            3814..5838
                     /gene="paaZ"
                     /locus_tag="VG274_03235"
     CDS             3814..5838
                     /gene="paaZ"
                     /locus_tag="VG274_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HWE05694.1"
                     /translation="MKSLQSYALDRWVTPQGQLTDIRSALTGETVAQIGTTVRDFGAV
                     LAHARDTGGPALRKMTFHERARILKALAEAVIARKQELYELSFDTGATRPDGWIDIEG
                     GAGTLFAYASKGRRELPDDTIVTEGAVEGLSKRGTFIGQHVMTPLHGAALHINAFNFP
                     VWGMLEKLAPTLLAGVPAIVKPASASAYVAETAFRILIDTNLLPNGALQLIVGPIGDL
                     FDHLGGQDVVSFTGSATTAEKLQRHPVVARESVRFIAERDSLNAALLAPDAEPGSPEF
                     DLFIREIVREMTSKSGQKCTAIRRALVPSGVIDAAESALKKALADIVVGDPRAEGVKM
                     GPLVSLPQRDDVRGKITELTHEARIVFGDPMRVEVRGGDDVKGAFLSPVLLRCDAPAK
                     ASRVHDTEPFGPVATLMPYHDLAEAIALADRGKGSLVMSLFTYDPAAAREVIAGAGAF
                     HGRLLIVDRDCAKESTGHGSPLPALVHGGPGRAGGGEEMGGLRGVFHYMQRTALQGSP
                     RMLAGITKSWISGAPEERTGPHPFRLKFSEVEPGKTLVTEPRIVTLEDIEHFAHFTGD
                     TFYAHMDEAAAAANPFFGGRVAHGYLLLAFAAGLFVDPAPGPVLANYGLDNLRFLKPV
                     KPGDAVRVQLTVKSKTPRNAQYGEVRWAVTLTNQSGETVATYELLTMNAM"
     gene            complement(5850..>6634)
                     /locus_tag="VG274_03240"
     CDS             complement(5850..>6634)
                     /locus_tag="VG274_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWE05695.1"
                     /translation="VPVSGDRLSREDIEAEIARRATATQPAGVGVTIVPHNSMRRRIA
                     DHMAQSLRTAPHVTALFEADFSTIIAHRTAHKASFADQGANLTFTAYFIFASVAAMKA
                     VPAVNARWYDDRIEIVDDVNVGIGTALGDKGLIVPIIHRAQTLSLVEIAQRLTEITEK
                     ARSGGLSPADVRNGTFSISNHGVSGSLLAAPIIINQPQSAILGVGKLQKRAVVREHSG
                     HDNMEVRPMAYVTLTIDHRVLDAHQTNAWLTRFVETIENWPR"
BASE COUNT         1202 a         2111 c         2102 g         1219 t
ORIGIN      
        1 gcaggtccgc gttcgcccat gccgagaact ttccggccgg catctgcttg atcgcgatgc
       61 ggatttcgtt ttcggccggc gagacgcaga ccgaataggt gcgccgcacg tcctcgccgc
      121 cgatttccgc tttcagcgtc aggtgctggc cggcctgaaa agcaaaggct tcgcgcaatt
      181 cggcggggac atcgaagcga atggaaaccg catccgccgt ctcgcggcgc acctcggcga
      241 tcctgagcga atgaaagccc ttgttaagcg acatgcggac tcagtggcac ttgaaacggt
      301 cgaagggctc gccgcaggcc agacaacgat tgagcgattt gcagggcgtc gagccgaagc
      361 ggctgatttc ctccgtatgc agagaaccgc agcgcgggca cgccgcctgt tcgtcgcgca
      421 ggctgtacct tgccgcgccg tggggcggcg gcgcgatgcc ataggcgtgc agcttttcgc
      481 ggccctcgtc actgatccaa tccgttgtcc aggcaggcga aaggaccgtt tcgatccgca
      541 accggctcag tccggcgcga tcaagcgccg cccgaatggc cgacgcgatg gcaagcgtgg
      601 cagggcagcc cgaataggtc ggggtgatga ccagcgtgcc ctcgcgcacg tcgcgcacga
      661 tgccgagatc gacaaccgat acgcagggaa tttccggatc cggcacatgg ctcaagagct
      721 cgaacacgcg atccttgagc gcctcgaccg attgagcgcg ggcagcggcc ttcaccacac
      781 ggctcccgga taggcgcgag gcaggaactg catctccgta agaaggtggc ccagatgctc
      841 ggaatgatgg cccgatcggc cgcctgaagc cgcacggcgc ggtttcggcg cttcgagtgt
      901 tgcttcggca agcgtttggg cgatgcgggc atcccattcc gcgcgcagcg ggcgcgggtc
      961 cacggcgaca ccgcgcacga tcagttcttc catagtcgcg tcgacggcga agagttcatc
     1021 cgtaaaccgc cacatccatt cgagtccggc aatgatgcgt ttgcggcttt cctcggtgcc
     1081 gtcgccaagg cggaccatcc actcgttcgc aaagcgcgca tgatagacga cttccttcac
     1141 cgccttgccg gcgatggcag agagcgtttc gtcctgcgat ctcaccagtt cgcgcatcag
     1201 gagttcctgc cagttcgaaa acaggaactg ccgcgcgaca gtctgcgcaa aatcgccgtt
     1261 aggctgctcg acgagcaacg cattgcggaa atcgaggaca tcgcgatgaa aggcaagaac
     1321 gtcggcatcg cgtccctcgc cctccgcttc gcccgcgagt ctcaggaaat gcgatgactg
     1381 gccgatcaga tcgaggccga tattggcgag cgcgagatcg atttccagca tcggcgaatg
     1441 gccgcaccat tcggacaact gctggccgag cacgagaccg tcgtccccca gacgcaacgc
     1501 atattccacg agcggcttca tcagatgttc ttgatgttct cggggatcga atagaacgtc
     1561 gggtgacgat agactttatc cgccgcaggc tcgaacaacg agcctgcctc gcccggatcc
     1621 gacgccacga tggatgcgga aggcacgacc cacaggctga caccctccat gcgcctggta
     1681 taggtgtcgc gcgccgcctc gatcgccatg cgggcgtccg ctgcatggac gctgccggcg
     1741 tgcttgtgcg cgagcccgtt cctggcccgc acaaagactt cccatagcgg ccattccttg
     1801 ttcattcgtc ctcactccgc cgcaagccgg ccattttgct ttgccgaatg tgcaagcgcc
     1861 gcttcgcgca cccaggcgcc atcctcccac gccatgaccc gcgcgttcat gcgctccttt
     1921 gcgaccggac cttcgccacg caccaccgcg tagaattctt cccagtcgag ctcgccaaaa
     1981 tcgtacgact cgcgcgcttt gttccatttg agtgcgggat cggggacgcg cagcccgatc
     2041 gcttcggcct gcggcaccgt cacgtcgacg aatctctgac gcagctgatc gttggtctcc
     2101 cgcttgatgc gccaacggat cgattgcgcg gaattgggcg aattgtcgtc cggcggcccg
     2161 aacatcatca gcgagggcca cccccagcgg tcgagcgcgt cctgcgccat ccgcttctgt
     2221 tccggcgagc cggcggcgag cgtcatcgtg atctcatagc cctggcgctg atgaaagctt
     2281 tcctctttgc agatgcgcac catcgcgcgc gcataaggcc cgtaggacgt gcgctgcagc
     2341 ggcacctggt tcatgattgc cgcgccatcg accagccagc cgatcgcccc gatatcggcc
     2401 caggtgaggg tcggataatt gaagatcgtc gaatatttgg cgcggccgga atgcagtgct
     2461 tcgatcatct cgtcgcggct ggtgccgagg gtttcggccg ccgcatagag atagagaccg
     2521 tggccgccct cgtcctgcac cttggcgagc agcaccgctt tgcgacgaag actcggcgcg
     2581 cgcgtgatcc agttcccctc gggcagcatg cccacgatct cggaatgggc gtgctgggaa
     2641 atctggcgta tcagcgtctt gcggtaggcc tcgggcatcc agtcctttgg ctcgatgaaa
     2701 tcgtcggcgg caacccgcgc ctcgaacgcc gcgagccgca ccggatcttc attggccatt
     2761 gcagccgccg gctttttgtt tttgtcgctc agctcggtcg tatacatcgc ccgagaattc
     2821 cttcctgaat gcatcgtccg cagactacaa ttaatcgaac gagcggtcaa ttaattcgtt
     2881 ccgggcgcga tgcattgtgt tcttcatctg cgcgcgggcg caaaattgac cgttcgggcc
     2941 ttctatccta agcctgcccg ccaatggatg cgggccaacc cgcgcattcc cgggaggtgc
     3001 ggcgatggcg tatgacacga ttctgctcga tatcgcgaag ggcgccgcgc ggttgacgct
     3061 caaccggcct gaccgcctga acagcttcaa tgtcgaaatg catcgtgagg ttgcggaagc
     3121 gttgtcgcgc gtcgaggcag atccggaaat tcgcgccctt ttgatcaccg gcgccggacg
     3181 tggcttttgc gccgggcagg acctttcgga tcgcgcggtc gcgccgggcg ccgacggcgt
     3241 cgatctgggg gagtcgctcg aaaatcgcta caatccgctt atccggcggc tcatcgggct
     3301 gccgaagcct gtggtctgcg cggtgaatgg cgtggcggcg ggagccggcg ccaatatcgc
     3361 ctttgccgcc gacatcgtgc ttgcggcgaa gagtgcgaaa ttcatccagt ccttctcgaa
     3421 tatcgggctc attcccgatt ccggcggcac gtggattctt ccgcggctgg ccggacaggc
     3481 gcgtgcgctc ggcctggcgc tcaccggcga gccgctcggc gccgaaaagg ccgaaagttg
     3541 gggcctgatc tggcgcgtct tcgatgacga tcaactcgcg ggcgaagcgg agaaactcgt
     3601 cgaacgcctg gcggtcggac caacgcgagg ctttgccgcg atcaagatgg cgatgcgcaa
     3661 gggctggctc gcgggcctcg aagaacaact cgatctcgag cgcgacctgc aacgcgacct
     3721 tggacgctcg ggcgactatc gcgaaggcgt cgcggccttc atggaaaagc gcaggccgaa
     3781 cttcagcggc caataaacca gggaaggctc ccgatgaaat cccttcagag ctacgcgctc
     3841 gatcgctggg tgacgccgca gggacagttg acggatatcc gctccgcctt gaccggcgag
     3901 acggtagcgc agatcggaac cactgtccgc gatttcggcg cggtgcttgc ccatgcgcgc
     3961 gatacgggcg gaccggcact ccgcaaaatg acgttccacg agcgcgcgag gattctgaag
     4021 gctctcgccg aagccgtcat cgcgcggaag caggaactct acgagctctc cttcgacacg
     4081 ggcgcaacca ggcccgacgg ctggatcgac attgaaggcg gcgcgggaac gctgttcgcc
     4141 tatgcgtcga agggccgtcg cgagcttcct gacgacacga tcgtgacgga aggcgcagtg
     4201 gaagggcttt cgaaacgcgg caccttcatc ggccagcacg tgatgacgcc gctgcacggc
     4261 gcggccctgc acatcaatgc cttcaatttt ccggtatggg gcatgctgga aaagctggcc
     4321 cccacgctgc ttgcgggcgt gcccgccatc gttaaacccg caagcgccag cgcctacgtc
     4381 gcggaaaccg cgttccgcat cctgatcgac acaaatcttc tgccaaacgg cgcgctgcag
     4441 ctgattgtgg gaccaatcgg cgatttgttc gaccatctcg gcggacagga cgttgtctct
     4501 ttcactggct cagccacgac ggcggagaag cttcagcgcc acccggtcgt cgcacgcgaa
     4561 agtgtgcgct tcatcgccga gcgcgactca ctgaacgccg cgctgctcgc gcccgatgcg
     4621 gaacccggct ccccggaatt cgatcttttc attcgcgaaa tcgtgcgcga aatgacgtcg
     4681 aagtcgggcc agaaatgcac ggccatccgc cgcgcgctcg taccttccgg cgtcatcgat
     4741 gcagccgaga gcgcgctcaa gaaagcgctg gccgacatcg tggtcggtga tccgcgcgca
     4801 gagggcgtga agatggggcc gctggtcagc ctgccgcagc gcgacgacgt gcgcgggaag
     4861 atcacggagc tgacccacga agcgcgcatc gtgttcggcg atccgatgcg cgtcgaggtt
     4921 cgcggtggag acgatgtgaa aggcgccttt ctgtcgcccg tccttctgcg ctgcgacgcg
     4981 ccggcgaaag cgtcccgcgt gcacgacacc gagccgttcg gccccgtcgc gacgctgatg
     5041 ccctaccacg atcttgccga ggcgatcgcg cttgccgacc gcggcaaggg ttcgctggtc
     5101 atgtccttgt tcacttacga cccggcggcc gcgcgcgaag tcatcgcagg cgcgggagcg
     5161 ttccacggca ggttgctgat tgtcgatcgg gactgcgcga aagagtccac cggccatgga
     5221 tcgcctctgc cggccctcgt ccatggcggt ccgggccgtg cgggcggcgg cgaagaaatg
     5281 ggcgggttgc gtggcgtctt tcattacatg cagcggacgg cgttgcaggg ctctccgcgc
     5341 atgctcgccg gcatcacgaa gagctggatt tcgggcgcac ccgaagaacg caccggccca
     5401 catcccttca ggctgaaatt cagcgaggtc gaacccggca agacgctggt gacggaaccg
     5461 cgcatcgtga cgctcgaaga catcgaacat ttcgcccatt tcaccggcga tacgttctac
     5521 gcccacatgg atgaagcggc agcggcggca aatccgttct tcggcgggcg cgtggcacat
     5581 ggatatttgt tgttggcctt cgccgcgggt ctgttcgtcg accccgcgcc ggggcccgtc
     5641 ctggcaaatt atggcctgga caatctgcgt ttcctcaagc cggtgaagcc cggcgatgcc
     5701 gtccgcgtac agctgacggt gaaatcaaag accccgcgca acgcgcaata cggcgaggtc
     5761 cgctgggcag tgacgttgac caaccaatcc ggcgagacgg ttgcgaccta cgaattgctg
     5821 acgatgaacg cgatgtgacg tgtggccggc tatcgcggcc agttctcgat cgtctcgaca
     5881 aatcgcgtca gccacgcatt ggtctggtgc gcatccagga cacggtgatc gatggtgagg
     5941 gtgacatagg ccatcgggcg gacttccatg ttgtcgtgac cggaatgctc gcgcaccacc
     6001 gcgcgcttct gcaatttgcc gacgcccagg atcgccgatt gcggctggtt gatgatgatc
     6061 ggcgccgcga gaagcgaacc ggaaacgccg tgattggaaa tcgagaacgt cccattccgc
     6121 acatctgcgg gtgaaagacc gcccgagcgc gccttctccg ttatctccgt caaccgctgc
     6181 gcgatctcga cgagcgacag cgtttgggca cggtgaatga tcggcacgat aagacccttg
     6241 tcgccgagtg ccgtgccaat gccgacattg acgtcgtcga cgatttcgat gcggtcgtcg
     6301 taccagcggg cgttgaccgc gggaacggct ttcatcgctg caacagacgc gaaaataaaa
     6361 taggcggtga aggtgaggtt ggcgccctgg tccgcaaagg aagctttgtg cgcggtgcgg
     6421 tgcgcaatga tggtgctgaa atcggcttcg aacagcgcgg tcacgtgcgg cgcggtgcgc
     6481 agcgattgcg ccatatgatc ggcgatgcgc cgacgcatgg aattgtgcgg cacaatggtc
     6541 acgccgacgc cggccggctg tgtggccgtc gcgcggcgtg cgatttcggc ttcgatgtct
     6601 tcgcgcgaga gcctgtcgcc gctgaccggc accg
//
LOCUS       DASZMC010000163         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1173154,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000163 DASZMC010000000
VERSION     DASZMC010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173154"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VG274_03245"
     CDS             <1..313
                     /locus_tag="VG274_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HWE05696.1"
                     /translation="ITLAVAGFSPDEISITAEQNVVTIEGSKADKPGAEFLYRGISTR
                     QFKRQFSLADYVQVKGAAFDNGLLRIELLREIPEAMKPRRIAINGAKADNVHQIEAKA
                     A"
     gene            400..723
                     /locus_tag="VG274_03250"
     CDS             400..723
                     /locus_tag="VG274_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05697.1"
                     /translation="MRPMTATNDNVFDINALLHPGTTFEHPRDVVSHPGLSLAEKRAI
                     LASWASDASAIASCPALRAPDGLKAVVTIDEILEALYALDGGPRNPPGGKPMRLRSIA
                     RCAAA"
     gene            1736..>2004
                     /locus_tag="VG274_03255"
     CDS             1736..>2004
                     /locus_tag="VG274_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWE05698.1"
                     /translation="MTGDYLPFTSLDKASGKFRGFDVDVAEALGKALGVKVEFVQTAW
                     PQMMKDFEADNFDIVMGGVSITMDRQKKGFFSTAIMREGKTPIAR"
BASE COUNT          403 a          641 c          612 g          348 t
ORIGIN      
        1 gatcacgctg gcggtcgcag gattctcgcc cgacgagata tcgatcaccg ccgagcagaa
       61 cgtcgtcacc atcgaaggca gcaaggccga caagcccggt gccgagttcc tctatcgggg
      121 aatctcgacc aggcagttca agcggcagtt cagcctggcg gactacgtgc aggtcaaggg
      181 cgcggctttc gacaatggat tgctccggat cgaactgctg cgcgaaatcc ccgaagccat
      241 gaagccccgg cggatcgcca tcaacggcgc aaaagccgac aatgtccatc agatcgaagc
      301 caaggcggcc tgaacaaagc tgccggaaac gttcgtgccg cgccccatgg cgcggcacac
      361 cacgcacaga gaaaattcgt ccaacctttg gaggcgacca tgcggccgat gaccgccact
      421 aacgacaacg tattcgacat caacgctctt ctgcatcccg gcaccacgtt cgaacacccg
      481 cgggatgtgg tttcccaccc aggcctcagt ctcgccgaaa agcgtgcgat ccttgcctct
      541 tgggcctcgg acgcgtcggc gatagcgtcc tgtccagcct tgcgggctcc ggatggactg
      601 aaggcagtcg tcaccatcga cgaaattctg gaggcgctgt acgcactgga tggcggacca
      661 cggaatccgc cgggcggcaa gccgatgcgg cttcgctcga tcgcgcggtg cgctgcagct
      721 tgaacagcgt ctcgctcatg gagattgcag gatgagtgga gcgcagtcgc acggccctca
      781 tgccggctat ggctatgacg cgcgtgactc tacccatcct gcgagtgcga atttatccgg
      841 ccgcgccgct agggctagcg gcgcagatcc cgcgactaca ccacggcttc gtcaatccaa
      901 ttttctgccg gcgagcgcgt gttcctcgct tgacccaaaa ggcaaatcac cgacctatct
      961 ccctctgtcc cgcgccccca atgaggggcg tatcatgatc gtcacgaacg ttgggcgtgg
     1021 gatgcggtgg acgcggcagc gtcgtcgcgc gatgcaagcg cagggccggt tggaaagccg
     1081 gtgagcgcgc gcttcgcgcg tgaacgagcg gcgctgcttc cgttatcgcc aacgcctcgg
     1141 ttgatgtgca cttgctccga agtctcttgg cgaatgacag atcgcgtacg gcaaaaccgt
     1201 gtggtcctgg cacccgttgc tggtgtcaag ccggtggagg cgaagtcgac ccaaccgggt
     1261 tcgatcagcc atattcgccg gcgacggtga caagacgaat tcgtcgccgg ggagagcgcg
     1321 gcataagccg ttaaaaccat tgcgcaggga aatgccgggt gtccggagta ctgactaccg
     1381 tgtgcatttt tgctgcacac gggctgcggg tgccccgcgc acccggcatt ccctgcgccc
     1441 tcatccttcc gatggcgcca gcgacgacaa ataactcggg cctccatgcc gcgagagcgc
     1501 accggcatga ccggacgaaa atcgacggga aacccatgcg cggggcgcaa tagcgcggca
     1561 tatcgcatcg tgctaggcgt gcccgctcgt cccgcaacca agaagaagta caaaaattct
     1621 caaggccctt actgctctcg ccctcagcct tttcctcgtc gccaactccg cccacgcgca
     1681 acagcagccc tcgcgacttg acgacatcgt caagcgcggc acgctccgcg tcggcatgac
     1741 cggcgactat ctgccgttca cctcgctcga caaggccagc ggaaaatttc gtggcttcga
     1801 cgtcgacgtg gcggaggccc tgggcaaggc gctcggggtc aaggtcgagt tcgtgcagac
     1861 ggcgtggccg cagatgatga aggatttcga ggccgacaat ttcgacatcg tgatgggcgg
     1921 cgtctcgatc acgatggacc ggcaaaagaa gggattcttc tcgaccgcga tcatgcgcga
     1981 gggcaagacg ccgatcgcgc gctg
//
LOCUS       DASZMC010000164         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2546571,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000164 DASZMC010000000
VERSION     DASZMC010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2546571"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VG274_03260"
     CDS             <1..296
                     /locus_tag="VG274_03260"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWE05699.1"
                     /translation="KTRPTERIAQEVRGFFAACSAEGVYAGGVHLEMTGKNVTECTGG
                     ARVISEADLHDRYHTFCDPRLNAEQSVEMAFMVADLLKVQRLAAPARPVRAAE"
     gene            486..1592
                     /locus_tag="VG274_03265"
     CDS             486..1592
                     /locus_tag="VG274_03265"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWE05700.1"
                     /translation="MRLSEYAIGRDNNFTLLRFSAAMTVLFAHSVAVLGLPPEREFFF
                     NRLGFSLGEMGLDMLFVTSGFLVTASLVGRQDLIAYLWARILRVYPGLLIMLVLTVFV
                     LAPALTTLPSDAYYTSSLTWEYFRKCATLIGGVRYSLPGVFENVPLKGQFNGSLWTMP
                     VEVRMYLYLAAIWVALAVVPALRLKAMRVVLPISAAVLIAIVLSARLTGGAYNGANIR
                     VFMFLYGSSLFVWRDRIPVGPWLLAGILAALLLASFDKTVFFVVYAACLAPLVLHLVY
                     VPGGRIRAFNDWGDYSYGVYIYAFPVQQTLAFLFPAMSLGAMMASSAVVSVAIAIVSW
                     KLIEERALGLKGDCAEATWRAFDLGLAKIVGVVR"
     gene            complement(1745..>2006)
                     /locus_tag="VG274_03270"
     CDS             complement(1745..>2006)
                     /locus_tag="VG274_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05701.1"
                     /translation="FEAQSDCDAEIEKFDLTLAEIPKSAAFRAAQAFRLYRRGGGVRT
                     GALPDFFIGAHASVAAAPLLTRDVRRYRAYFPELTLVAPEGA"
BASE COUNT          316 a          615 c          631 g          444 t
ORIGIN      
        1 acaagacaag gccaaccgaa cgcatcgccc aggaagtgcg cggtttcttc gccgcctgca
       61 gcgccgaggg tgtctatgcc ggcggcgtcc atctcgaaat gacaggtaag aacgtcaccg
      121 agtgcaccgg cggagcgcgc gtcatctcgg aagcggacct gcatgaccgg tatcacacct
      181 tttgcgatcc gcgcctcaat gccgagcagt cggtcgagat ggctttcatg gtcgcggatc
      241 ttcttaaagt ccagcggctc gccgcgcccg cgcggcctgt gcgggcggcc gagtaagcct
      301 ttgtcagtga agccagagtc gtcattgcga gcggagcgaa gcaatccagg ggaacgaagg
      361 gcgccctacg gtcctctgga tcgtcacgtc gccatagccc gtcgaaaaga cgggcgtctc
      421 tcgacgccct acggctcctc gcgatgacga atccggccaa gagcaaccag ctctaggggc
      481 gcggggtgcg gctctccgaa tacgccatcg ggcgggacaa caatttcacg ctgctccgct
      541 tttctgcggc gatgacggtg ctgttcgcgc acagcgtcgc ggtgctgggg ctgccgccgg
      601 aacgcgaatt ctttttcaac cggctcgggt tttcgctcgg cgagatgggc ctcgacatgc
      661 tgttcgtgac gagcggcttc cttgtcaccg cgagcctggt cggacggcag gatctgatcg
      721 cctacctctg ggcgcgcatc ctcagggtct atcccggcct gttgatcatg ctcgtcctga
      781 ccgtgttcgt tcttgcgcct gcgctgacga cgctgccaag cgatgcgtat tatacgtcgt
      841 ctttgacgtg ggaatatttc cgcaaatgcg cgacgctcat cggcggcgtg cgctattcgt
      901 tgccgggcgt gttcgagaat gtgccgctca agggccagtt caacggatcg ttgtggacga
      961 tgccggtcga agtgaggatg tatctctatc tcgccgcgat ctgggttgcg ctcgcggtcg
     1021 tgcctgctct gcgtcttaag gccatgcggg ttgtcttgcc gatctcggcg gccgtgctga
     1081 tcgcgattgt gctgagcgcg cggttgaccg gcggcgcgta taacggcgcg aacattcgcg
     1141 tcttcatgtt cctctacgga tcgagcctct tcgtttggcg cgaccggatc ccggtcggtc
     1201 cctggctgct cgccgggatt ctcgcggcgc ttctcttggc gtctttcgac aagacggtct
     1261 tctttgtcgt ctacgcggcc tgcctcgcgc cgctcgtcct tcatctggtc tatgtgcccg
     1321 gcggccgcat ccgggcgttc aacgactggg gcgattattc ctacggcgtt tatatctacg
     1381 cgttccccgt ccagcagacg ctggcctttc tcttcccggc catgtcgctt ggagcgatga
     1441 tggcttcgtc cgcagtggtt tcggtcgcga tcgcgatcgt gtcgtggaag ctgatcgagg
     1501 agcgggcgct tggcctgaag ggcgactgcg ccgaggcgac gtggcgggcc ttcgatctgg
     1561 gtttggcgaa gatcgttggc gtcgtgcgct gaccccgccg ggcatggttc ggcgtcttga
     1621 gcggttctgg cgccgacaga gcgatttcaa ggcgttaggc gccaaaacct gtcccgtttg
     1681 cctttcgcgt ttgtgcaact cctccggcta aataacctaa ctctcacgta gataattccg
     1741 gagcctatgc cccttccggc gcgacaagcg tcagctccgg aaaataggcg cgatagcgcc
     1801 gcacgtcgcg cgtaagcaat ggagcggccg ctacgcttgc atgcgctccg atgaagaagt
     1861 cgggtaatgc gccggtccga actccgccgc cgcgccgata caatctgaaa gcttgcgcgg
     1921 cgcgaaaggc ggctgattta ggtatttccg ctagtgtcag atcgaatttc tcgatctccg
     1981 cgtcacaatc cgactgcgcc tcgaac
//
LOCUS       DASZMC010000165         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3087464,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000165 DASZMC010000000
VERSION     DASZMC010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3087464"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1834
                     /locus_tag="VG274_03275"
     CDS             <1..1834
                     /locus_tag="VG274_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWE05702.1"
                     /translation="DGIDIRGLLRAAWMNLRAGHVVAGGSTITQQTAKIVFLSPKRTF
                     VRKYEELLDAAALEKSLSKKQILELYLNRIYLGAGAYGVDGAAHVYFGKSARQLSLSE
                     AAMLATLTRAPSAFSPRRDLAAAQERAGLVLRAMLHTGAIARDAADEAIQHPAIISDR
                     ALADARNFFLDTAADQALKLIGSDGEGRDTDLIVHTTLEPRLQEAARHALAHTLNAHG
                     KRAHASEGAIVLMKPDGAVSALIGGRDYDSSVFNRATQAKRQPGSAFKPFVYLAALEN
                     GITPWDVREDAPVDIGGWTPTNYGGRSYGTVTLADALAHSINTVTAGLAQEVGITSVV
                     DAAHRCGISSPLAPNASLALGTSVVTPIELTAAYAVFASGGERVSPYLVTGIEDRSHR
                     NLYQRKPPAPDRIIASHVDRDLTGMLYGVVVEGTGRAAALPGREAAGKTGTTQDYRDA
                     WFVGFTPDYVAGVWVGNDDNSPMRNVTGGTLPAAIWKDVMIAAERGLPAKALEKSSEP
                     VAEDVSDTESASTDGDDSNADSSTASSDASTRQSGTDDEAAQQQQQQPPPAQNGTSSF
                     WDWLTGNGRDRSGEQPHASEEDSKEQPRASTATEPPPNRVEAAP"
BASE COUNT          378 a          653 c          602 g          301 t
ORIGIN      
        1 cgacgggatc gacattcgcg gactcctgcg cgccgcttgg atgaacctgc gcgccggcca
       61 tgtcgtggcg gggggatcga cgatcacgca gcagacggca aagatcgtgt tcctgtctcc
      121 caaacgcacc ttcgtgcgca aatatgaaga gttgctcgat gccgcggcgc tcgaaaaatc
      181 tctctccaag aagcaaattc tggagctcta tctcaatcgt atctatctgg gtgccggagc
      241 atacggagtc gacggcgcag cccacgtcta tttcggcaaa tcggcgcggc agctctcgct
      301 gtccgaagct gcgatgctgg ccactctcac ccgcgcgcca tccgcctttt cgccgcgacg
      361 cgaccttgcc gcagcgcagg aacgagccgg actcgtgctg cgtgcgatgc tgcatacggg
      421 cgcaatcgcg cgcgacgcgg ccgatgaggc aatccagcat cctgcgatca tcagcgaccg
      481 cgcgctcgcc gatgcgcgga atttctttct cgataccgcg gccgaccagg cgctcaagct
      541 catcggttcc gacggcgagg gccgcgatac cgatctgatc gtgcacacga ctctcgagcc
      601 gcggctgcag gaagcggcgc ggcatgcgct ggcgcacacc ctgaacgcgc atgggaagcg
      661 cgcgcatgcg agcgaaggcg caatcgttct gatgaagccg gacggagctg tttccgccct
      721 gatcggcggg cgcgattacg attcgagcgt gttcaaccgg gcgacgcaag ccaagcgcca
      781 gcccggttcc gccttcaagc cgttcgtcta tctcgcggcg ctcgagaacg gcatcacgcc
      841 ctgggacgtg cgcgaagacg cgcccgtgga catcggcggc tggacgccta ccaattacgg
      901 cggacgcagc tacggcaccg taacgctcgc cgacgcgctc gcccattcca tcaacacagt
      961 gaccgcgggc ctcgcccagg aggtcggcat cacaagcgtc gtcgacgccg cgcaccgctg
     1021 tggcatctct tctccgctcg cgccaaacgc ttcgctcgcg ctcggcactt cggtcgtaac
     1081 acccatcgaa ctgacggcgg cctatgccgt gtttgcgtcg ggcggggagc gcgtctcgcc
     1141 gtatcttgtc accggaatcg aagatcgttc gcaccggaac ctgtatcagc gcaaacctcc
     1201 ggcacccgat cggatcatcg cctcgcacgt cgatcgcgat ctcacgggga tgctatatgg
     1261 cgtcgttgtc gaaggaacgg gacgtgcagc cgcccttccc ggccgcgaag cagcgggaaa
     1321 aaccggaacg acccaggatt atcgcgatgc ctggttcgtc ggcttcacgc cggattatgt
     1381 ggcgggcgta tgggtcggca acgacgacaa ttcccccatg cgcaatgtga cgggcggaac
     1441 gttgcctgcc gccatctgga aagacgtcat gatcgccgcc gaacgcggac tgccggcgaa
     1501 ggcgctcgaa aagtccagcg agcctgtggc cgaggatgtg agcgatacag aaagcgccag
     1561 cacggacgga gatgattcga atgccgattc ctcgacggcg tcgagcgatg ccagcacgcg
     1621 ccaatccggt acggacgacg aagcggcgca acagcagcag cagcagcctc cgccagccca
     1681 gaacgggaca agctcattct gggactggtt gacgggcaat ggtcgcgaca ggtcgggcga
     1741 acagccacac gcctccgagg aggattccaa agagcaaccc cgcgccagca ctgcaaccga
     1801 accgccgccg aaccgcgtgg aagccgcgcc atagcgcagc gtcgagtcca cgccgcctcc
     1861 gcccggacca gacatcgagg attctccgcc agtgcgcgat cccggggacc gtgcaggccc
     1921 gccaccagca aacg
//
LOCUS       DASZMC010000166         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1982285,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000166 DASZMC010000000
VERSION     DASZMC010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982285"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1464)
                     /locus_tag="VG274_03280"
     CDS             complement(244..1464)
                     /locus_tag="VG274_03280"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWE05703.1"
                     /translation="MSAGVVIIGGGQAGVEAASALRALGYAEPVSLISDEPEPPYQRP
                     PLSKEYLLGKIDAARLPLRAESFYTKHRIDLLLSQRVVDIGVAMKRVKLASGAVIPYD
                     FAILATGARNRILTGSGAGRVLYLRTKSDSDAVRQRLAEVESVAVIGGGFIGLEVAAA
                     ARSLGKRVIVLEAAPRLMSRAASPLISDFFRDMHRAEGVEVLLGAAISEIERDAVRLA
                     DGTSVPAQAIFAGIGVTPNVEQARDAELAVDGGISVDACLRTSDPNIFAIGDCAEFPN
                     PFLDGGASARIRLESVQNAVDQARYVARCIANGGGSPEPFSEIPWFWTDQFDVRFQMA
                     GLCARHDQIVVRGATESRKFSAFYFRQGRLVSADSVNRFGDHIAVRKILRARASLTQE
                     QAADESFDLKQLVG"
     gene            complement(1510..1833)
                     /locus_tag="VG274_03285"
     CDS             complement(1510..1833)
                     /locus_tag="VG274_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWE05704.1"
                     /translation="MPKVTYIAHTGESHTVEVPAGYSVMEGAVRNGIDGIVAECGGAC
                     MCATCHVFVDEAFLDRLPPIGDEQEEMLNATAVDRRPNSRLSCQIPSIPELDGLIVRM
                     PERQT"
     gene            complement(1850..2245)
                     /locus_tag="VG274_03290"
     CDS             complement(1850..2245)
                     /locus_tag="VG274_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWE05705.1"
                     /translation="MATLGLVEYQNATPEVRAVYDDIMATRQTDWINNFWKALANDPP
                     TLRRTWESIKQIMAPGALDPLTKEMIYLAVSSSNQCPYCIASHTAGARGKGMTDAQFA
                     ELMAVVGMANETNRLVSGYQVEIDESFRT"
     gene            complement(2274..2777)
                     /locus_tag="VG274_03295"
     CDS             complement(2274..2777)
                     /locus_tag="VG274_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05706.1"
                     /translation="MQVLQAGPHKLLLLELDREFIENIARQAGFEPRLEEHDRRVLID
                     LNAESRQAPLLLFDAADPANLGWFSRCQFYVDGMSGAVLQTPIQLANQRDRQGKALPN
                     SIRLQINKELPASFRMPNKAAVTEQVVYSVLYNFLNALVNTGVGVCGGTVVQPLAGRI
                     GPQENRN"
     gene            2850..>3276
                     /locus_tag="VG274_03300"
     CDS             2850..>3276
                     /locus_tag="VG274_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWE05707.1"
                     /translation="MTAGLVLFDIDGTLLRQAGPHHREALIEAVRRASGLRTTLDGIP
                     VAGMLDRDIATSMLRAAGAKEAAIRRAMPQIVEEAQKIYLRTCPDLRLKVCPGVRGLL
                     YKLARRGVVTGLVTGNLTHIGWTKMERAGLRHYFRFGAFS"
BASE COUNT          651 a          949 c         1059 g          617 t
ORIGIN      
        1 agaccgtggt gaccgaagga cagctgaggc tggctccggg cagcaaagtg cttgtgcagg
       61 ataagacggg cgcggatggc ggcgttggaa cgggatcgct gacaggctcg caaggcggcg
      121 gaaagcccgc cggtcctcgc agctaacgtc cgcttcacgc tcacgttcac tctgcgaaag
      181 cagattcctt gatcgcttcg ctcctcagga tgacccgttt ttgcaacgat tttccggacg
      241 caactaccct accagttgtt ttagatcgaa ggattcgtcg gcggcttgtt cctgcgtgag
      301 gcttgcgcgg gcgcgcagta ttttgcgcac cgcgatatga tcgccgaagc gattgacgga
      361 atcagccgaa acgaggcgcc cctggcgaaa atagaacgcc gaaaacttgc ggctctccgt
      421 tgcgccgcgg accacgatct gatcgtgacg agcgcacaaa ccggccattt gaaagcgaac
      481 atcgaactgg tcggtccaga accagggaat ttcgctaaag ggctcaggcg agccgccgcc
      541 attggcgatg caacgcgcga cgtacctggc ctgatcgacc gcattctgca cggattcgag
      601 acgaatgcga gcgctggcgc cgccgtcgag gaacggattc gggaattccg cgcagtcgcc
      661 gatggcgaaa atgttcgggt ccgacgtgcg gaggcatgca tcgacagaga ttccgccatc
      721 gacggcgagc tcggcgtcgc gcgcctgctc cacattgggc gtgacgccga tacccgcgaa
      781 gattgcctga gcgggtacgc tagtgccgtc cgcgaggcga accgcgtcac gttcgatttc
      841 cgaaatggcg gcgcccagca gaacttcgac gccttcggcg cgatgcatgt cgcggaagaa
      901 atctgaaatc aggggagatg cggcgcgcga catcagccgc ggcgcggctt ccagcacaat
      961 gacgcgcttg ccgagagaac gcgcggcggc agcgacctcg agcccgatga accctccgcc
     1021 gatgacggcg acgctttcga cctcggccag acgttggcgc acggcgtcgg aatcggattt
     1081 agtgcgcaaa tagaggacgc gcccggcgcc gctgcccgtg agaatgcgat tgcgcgcgcc
     1141 cgtggcgaga atcgcgaaat cgtaagggat gacagcgccc gaggccagct tgacgcgctt
     1201 catcgccacg ccgatatcca cgacgcgctg gctcagcaac agatcgatgc ggtgcttggt
     1261 gtaaaacgat tcggcgcgca aaggaaggcg cgcggcgtcg atcttgccga gcagatattc
     1321 tttcgaaaga gggggccgct gatagggcgg ttcaggttcg tcgctaatca gggaaacggg
     1381 ttcggcatag ccaagagcac gaagcgcgga cgccgcctcg acaccagctt gcccaccgcc
     1441 gataatcact acccccgctg acatcggtcc tctgagagct tcccgctgcg ccgctgcaga
     1501 tgcgtttatt taggtttggc gttccggcat ccgcacgatg agcccgtcga gctccggaat
     1561 ggacggaatc tgacaactta agcggctgtt cggccggcga tcgaccgctg ttgcattgag
     1621 catttcttcc tgctcatcgc cgataggcgg caaacggtcc aggaacgcct catcgacgaa
     1681 cacgtggcag gtggcgcaca tacaggcgcc gccgcattcg gcgacgatgc cgtcgatgcc
     1741 gttgcgcacg gcgccttcca tgaccgaata tccggcggga acttccaccg tatgggattc
     1801 gcccgtgtgg gcgatataag taaccttggg catttctgaa aaatttcctt tacgtacgga
     1861 agctctcgtc gatttccacc tgatagccac tgacaagccg gttggtttcg ttggccatgc
     1921 cgacgacggc catcagctcc gcgaactgcg cgtccgtcat tcccttcccg cgcgcgccgg
     1981 cggtgtgcga ggcgatgcag tagggacact gattgctgga cgacacggcg aggtaaatca
     2041 tttccttggt aagcgggtcg agcgcgccgg gcgccatgat ctgcttgatg gattcccacg
     2101 tgcggcgcag cgtgggcgga tcgttcgcca gcgctttcca gaaattatta atccaatccg
     2161 tctggcgcgt ggccatgatg tcgtcgtaga cggcacgcac ttccggcgtg gcattttggt
     2221 attccaccag tcctaaagtt gccatgatcc gtgagcatcc cggcgcgcga gcgtcagttg
     2281 cgattctcct ggggaccgat gcgccccgcc agcggctgca cgaccgttcc tccgcagacg
     2341 ccgacgccgg tattcacgag cgcgttcaga aaattgtaga gcaccgagta gaccacctgc
     2401 tccgtgacgg cggctttgtt cggcatgcgg aaggaggcgg gcaattcctt gttgatctgc
     2461 aagcggatgg aattgggcag ggctttgccc tggcgatcgc gctgattggc cagctgaatg
     2521 ggagtctgca aaaccgcgcc cgacataccg tccacataaa actggcaccg cgaaaaccag
     2581 ccgaggttcg cgggatcggc ggcatcgaac aagagcagcg gggcttgccg gctttcggcg
     2641 ttcaggtcga tcagcacgcg gcgatcgtgt tcttcaagcc ggggctcgaa gccggcctga
     2701 cgggcgatgt tttcgataaa ctcgcggtca agctccaata ggagtaattt atgcggcccg
     2761 gcttgcaaaa cctgcatgca ttgtcatttt agcagtgcgt gcgcgataaa aacgaaaaga
     2821 tgcggcgaga aagggctatc gtaaagaaaa tgaccgcggg attggtgtta tttgacattg
     2881 acggaacgct gttgcggcaa gcagggccgc accacaggga ggcgctgatc gaagcggtgc
     2941 ggcgcgcgtc gggattgcgc acgacgctgg acgggattcc ggttgcggga atgctggatc
     3001 gcgatatcgc cacttcgatg ttgcgggcgg cgggcgccaa ggaggcggcg attcggcgcg
     3061 cgatgccgca gattgtcgaa gaagcgcaaa agatttatct gcgcacgtgc ccggatttgc
     3121 ggctgaaggt gtgtccaggc gtgcggggac tgctctacaa gctggctcgg cgcggtgtgg
     3181 tcacggggct ggtgacaggc aacctgacac acattggttg gaccaagatg gagcgcgcgg
     3241 ggctgcgcca ctactttcgc ttcggggcgt tctcgg
//
LOCUS       DASZMC010000167         9819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_65516,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000167 DASZMC010000000
VERSION     DASZMC010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65516"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1324)
                     /locus_tag="VG274_03305"
     CDS             complement(50..1324)
                     /locus_tag="VG274_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HWE05708.1"
                     /translation="MSRSLDDYVWRRMRITGGALVTYGSVEETIAAMKEIYFQPLDGL
                     REAIKARAGRFVSFANYDYLGLGDDPRVRQAAADAAISLGVGAEASRLVGGSRTVHDL
                     LEREIADFLGAEDAVTLVSGWMTNASLLGYLLTRSDLIVVDDLAHNSIVVGAEVSHAK
                     VLRFVHGDLDDLDALLAEHRPRAKRALIVVEGLYSMDGDMPDLHRVLELKHKHDAWLM
                     VDEAHSIGVLGATGRGITEHFGVDPREIDFTIGTLSKTFAASGGFIAGRKPVIEWLRF
                     TLPASMFSVGLSPVIAAAAREGLAILRSEPWRTARVQENSRLFLEEARARDLNAGAAA
                     GAGVISIQFPNNAVCMQASQALLQAGYYAPPIPIMAVPKNKPRIRFFVSAAHTPADIT
                     GVLDVLAAAMASSAHRAEGAHAEATAELNLGA"
     gene            complement(1806..1880)
                     /locus_tag="VG274_03310"
     tRNA            complement(1806..1880)
                     /locus_tag="VG274_03310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Lys,seq:ttt)
     gene            complement(2179..3291)
                     /gene="tgt"
                     /locus_tag="VG274_03315"
     CDS             complement(2179..3291)
                     /gene="tgt"
                     /locus_tag="VG274_03315"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWE05709.1"
                     /translation="MSGLRFEVQATCGAARLAILHTPRGDIATPAFMPVGTAASVKAM
                     LPEAVRSTGADIVLANTYHLMLRPGAERIARLGGLHRFMGWKGPILTDSGGYQVMSLA
                     QLRKISEEGVAFRSHIDGAEEFLSPERAMHIQRQLGSDIQMVLDECPAWPASETDIES
                     SLGLSLRWARRSKSAFGEQDGRGCFGIVQGGVFPHLRRRAAQELIAMDFDGHAIGGLA
                     VGEGQAAMFDVLEATEPHLPANRPRYLMGVGKPADIAGAVMRGIDMFDCVLPTRSGRN
                     GQAFTREGALNLRNARFADDEEPLDKACRCPACATFSRAYLHHVVKAGEIIASMLITW
                     HNLVHYQELMAGLRAAIAAGALPALVASLQTKDGRD"
     gene            complement(3288..4346)
                     /gene="queA"
                     /locus_tag="VG274_03320"
     CDS             complement(3288..4346)
                     /gene="queA"
                     /locus_tag="VG274_03320"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWE05710.1"
                     /translation="MDVSLFDFELPADRIALRPAEPRDSARMMVVRADGEVAHARIRD
                     LPNFLRRGDLVVVNDSKVVHARLRARRAARGAGEGAAIEILLHKRVAPDRFLAFARPA
                     RRLASGDTLDFHGTFAARVAAPRDGGEIELAFHIAGAELDQAISRHGEMPLPPYIASR
                     RAGDARDVIDYQTMFASHEGSVAAPTAGLHFTPALLTALDSAGIGVERITLHVGAGTF
                     LPVIADDTACHRMHGEWTRLSAQSAARICAARQAGGRIIAVGTTSLRTLETSASDDGA
                     VRGFEGETDLFVTPGYCFKAVDILLTNFHLPRSTLFMLACAFSGIERMKRAYALAIED
                     GYRFYSYGDACLLFRTTA"
     gene            complement(4374..5507)
                     /locus_tag="VG274_03325"
     CDS             complement(4374..5507)
                     /locus_tag="VG274_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HWE05711.1"
                     /translation="MPSIPFIDLQAQRRRLGAPLERAITAAVESGQWIMGPQVRALEE
                     QLAHFAGVKHAIACANGTDALLIILRAWEVGPGDAVFVPAFTFAASAEVVALVGATPV
                     FVDVLEDTFNMAPASLCAAIEMVRRDDDLRARVVMPVDLFGQAADYRALEPIAARGNL
                     LMLCDTAQGFGALLDGRRTGSIGDASSTSFFPAKPLGCYGDGGCSFTNDDGLAELLRS
                     IRVHGQGADKYDNVRIGVNSRLDTIQAAILLEKLKIFPEEIEMRERIAHRYSNAFTRS
                     NQIRIPRVIDGARSTWAQYTIQVPERDRLKADLDARGIPAQIYYLTPLSAQLGYRAFP
                     SAPLPVSTALGRTVISLPMHPYLDEATQDMIIEAVLESVGQTH"
     gene            complement(5623..6108)
                     /locus_tag="VG274_03330"
     CDS             complement(5623..6108)
                     /locus_tag="VG274_03330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWE05712.1"
                     /translation="MTDPDNMVVLTLKTGEVRIALRPDLAPLHVARIKELAGAKFYDG
                     HAFHRVIPEFMAQTGDPTGTGSGGSHLPRLKAEFSPERHLRGTLSMARAADPDSANSQ
                     FFICLADCPWLDRQYTVFGQVVDGMEHVDAIKKGGKHNNGAIAGEPDRIVTMRVGPGQ
                     S"
     gene            complement(6105..6620)
                     /gene="coaD"
                     /locus_tag="VG274_03335"
     CDS             complement(6105..6620)
                     /gene="coaD"
                     /locus_tag="VG274_03335"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWE05713.1"
                     /translation="MTRRHVGLYPGTFDPVTLGHLDIIQRAVKLVDHLVIGVATNPSK
                     SPMFTLAERVDMVRSETAPLGGSGRATISVETFDSLLMQFAEKVGASVIVRGLRAVSD
                     FEYEFQMVAMNQRLNPDIETVFLMADPRHQAIASKLVKEIAILGGNVAPFTTPHVAQL
                     LAERAQARKTP"
     gene            complement(6634..9348)
                     /gene="gyrA"
                     /locus_tag="VG274_03340"
     CDS             complement(6634..9348)
                     /gene="gyrA"
                     /locus_tag="VG274_03340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWE05714.1"
                     /translation="MSDIAAIGGPPGAGISHIAIEDELKRSYLDYAMSVIVARALPDA
                     RDGLKPVHRRILFSMHENGNDWNKPYRKSARIVGDVIGKYHPHGDQSVYDALVRMAQE
                     FSMRVPLIDGQGNFGSVDGDPPAAMRYTEVRRARIAHALTEDLEKDTVEFQDNYDATE
                     REPVVLPARFPNLLVNGGTGIAVGMATNIPPHNLGEVIDACLAWLDDPSISLDALTEI
                     VPGPDFPTGALIIGRLAARGALARGRGPILMRAKIHIEELRKDREAIIVTEIPYQVNK
                     ATMIERISELVRDKRVEGIADMRDESDRHGMRVVIELRRDASSEVVLNQLYRHSQLQT
                     SFNVNMLALNGGRPELMNLKGLIAAFLRFREEVITRRTRHELAKARERAHVLVGLAIA
                     VANIDEVIALIRGAADAATAKIQLMERGWPAKDVAPLVALVADPRHVVQPDGTIRLTD
                     EQAKAILEIRLQRLTALGRNELAEEVTKLGEAIKDYLDILRSRPRVLAIVRAELEEVR
                     KDFATPRVTALIDAEVEIEDEALIEREDVAVTVTHAGYIKRTPLAEYRVQGRGGKGRT
                     GMATREEDFVTHIFVASTHAPLLFFSSTGMAYKLKVWRLPEARIQGRGKAMVNLLPLA
                     EGERITTVLPLPEDEAQWEKLNVVFATRLGDVRRNELSDFVNVKANGKIAMKLESGDG
                     IVGVQTCTDADDLLLTTRAGKCIRFALTDVRIFKGRQSTGVRGIKLASGDEVVSLALL
                     HHVEAEIPETRAYLKQAGAVRRAATGEESEEVSEESEEGGEEAILTAERYAQFAAAEQ
                     FVLAVSATGFGKRTSSFEYRVTGRGGSGIVAIAMARKNNAIVAAFPVEESDDIMLITD
                     QGQTIRSPVASVRVAGRATQGVTLFRLETGERVVSVERIEETSAEAGA"
     gene            complement(9487..>9819)
                     /gene="ssb"
                     /locus_tag="VG274_03345"
     CDS             complement(9487..>9819)
                     /gene="ssb"
                     /locus_tag="VG274_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWE05715.1"
                     /translation="KQSGERRERTEWHRVVIFNEHLAKIAEQYLRKGSKIYLEGQIQT
                     RKWTDKDGAEKYTTEVVVPRFRGEITMLDSRADSGGGAAAAPSHSASAGDSPASFERN
                     EMDDEIPF"
BASE COUNT         1767 a         3173 c         3052 g         1827 t
ORIGIN      
        1 atgctggtct cctgcttcaa ggtaacccga tcggcgaggc cgcgacggct caggcgccga
       61 gattcagctc ggccgtcgct tccgcatgcg ccccctcggc gcggtgcgcg ctgcttgcca
      121 tcgcggccgc cagcacgtcg agcacgcccg taatatcggc cggtgtgtgg gctgcggaga
      181 cgaagaagcg gatgcggggc ttgttcttcg gaaccgccat gatcggtatc ggcggcgcgt
      241 aatagccggc ctgaagaagc gcctgggacg cctgcatgca gacggcgttg ttggggaact
      301 ggatcgaaat cacaccggcg ccggcggcag cgccggcatt cagatcgcgg gcgcgcgcct
      361 cctcgaggaa cagccgcgaa ttctcctgga cgcgcgcggt gcgccagggc tcgctgcgca
      421 ggatggcgag cccttcgcgc gccgcggccg cgatgacagg agaaagcccg acgctgaaca
      481 tgcttgccgg cagcgtgaag cgaagccatt cgatcacggg cttgcgcccg gcgatgaagc
      541 cgccgctggc cgcaaacgtc ttcgagagcg tgccgatcgt aaaatctatt tcgcgcgggt
      601 ccacgccgaa atgctccgtg atgccgcggc cggtcgcgcc cagcactccg atcgaatggg
      661 cttcgtcgac cataagccat gcatcgtgct tgtgcttcag ctcgaggaca cgatggagat
      721 ccggcatatc cccatccatg ctgtagaggc cctcgacgac gatcagcgcc cgcttcgcgc
      781 gcggccggtg ctcggcaaga agcgcatcca gatcatcgag atcgccgtga acaaagcgca
      841 gcaccttggc gtgtgaaact tcggcgccca cgacgatgct gttgtgggcg agatcgtcga
      901 ccacgatgag gtcgctgcgc gtaagcagat agccgagcag cgacgcattg gtcatccagc
      961 cgctgaccag cgtgacggca tcctccgcgc ccagaaaatc tgcgatctcg cgttcgagga
     1021 gatcgtgcac ggtccggctg ccgccgacga ggcgcgacgc ctccgcgccc acgccgagcg
     1081 atattgcggc gtccgcggcg gcctgccgga cgcgcgggtc atcgccgagc ccgagatagt
     1141 cgtaattggc gaagctcacg aaacgtccgg cacgcgcctt gatggcctcc ctcagaccgt
     1201 caagcggctg gaagtaaatc tccttcattg ccgcgatcgt ctcttcgacg gagccatagg
     1261 tcacgagcgc cccgcccgtg atccgcatcc tgcgccatac gtaatcgtcc aagctgcggc
     1321 tcatttctct cgtccctgaa cctcggctga cccggttatc cgcggcacca agctgtctgt
     1381 tctgcaaccg cgaccatgtt ccgccgtgcc catgcgaagc tccagaattc gtttcgcacc
     1441 cgcacccgac gggtcccggc attgtgggca catgccacat cggcaggctg gaacccgcta
     1501 cgggcgctca gtgcagccct ccattccggt atccctttga tactcggcag cagaagcgcc
     1561 tgcgaaattc cctttccgct tgcgccaccc gctaactgcc ggaagttagc cgctaatcgc
     1621 cgggggaggc aaccaaccgg ccaaagcctc gggacgccga aatcggccca ttggtttgtc
     1681 accggcggcc gaaaaacaat agggcggcgc cctacaagtt gcgaagaacc ttggggcttt
     1741 ttgcaatcct ggtgcatcgg gcgaagcgca gccggacggc cttcatcgtt gaatgcttga
     1801 ttttttggtg agcccggatg ggatcgaacc atcgaccctc tgattaaaag tcagatgctc
     1861 tacccctgag ctacgggctc gtctcgaaag gcggcggacc atagggggca ccccgccaac
     1921 ggtcaacacg cgggacgcgg taaacctact ggttcagatc atgacgattg aatcccaaaa
     1981 ctgtcatggc ccgcgcgtga agcttgtcct ccggccgcgc ttcgcgcgga tcggggggtg
     2041 ggccacccag acgagaccgc tccgagcgct ttttcagcgc gcggacgcgc gctggctggg
     2101 tggcctcccc cggtttaacc gggggaccag cgggggccat gacgattttg aggatacaaa
     2161 gggtttggat tcgatccatt agtcgcgccc gtctttcgtc tgaaggctcg cgaccagagc
     2221 aggcagagcc ccagctgcga tagcggcacg taatcccgcc atcaattcct gatagtgaac
     2281 gagattgtgc caggtgatca gcatgctggc gatgatctcg cccgccttga cgacatgatg
     2341 aaggtaggcg cgcgagaagg tcgcgcaggc ggggcatcgg cacgctttat cgagcggctc
     2401 ctcgtcgtcg gcaaagcgtg cattgcgcag attgagcgcg ccttcgcgtg taaaggcctg
     2461 gccattgcgg ccggaccgcg tggggagcac gcaatcgaac atatcgatcc cgcgcatgac
     2521 cgcgccggca atatcggcag gcttgccaac tcccatcagg taacgtgggc ggttcgcggg
     2581 caggtggggc tccgtcgctt cgagcacatc gaacattgcc gcctgcccct cgccgaccgc
     2641 caatcctccg atggcgtggc cgtcgaaatc catggcgatc agctcttgcg ccgcacgccg
     2701 gcgcagatga ggaaagactc cgccctgcac gatgccaaaa cagccgcggc cgtcctgttc
     2761 gccgaaggcc gatttgctgc gccgcgccca ccgcagcgaa agtcccagag aagactcgat
     2821 gtcggtttcg ctcgccggcc atgcgggaca ttcgtccagc accatctgga tatccgagcc
     2881 caactgccgc tggatatgca tcgcgcgctc gggagagaga aattcctccg caccatcgat
     2941 atgcgagcgg aaggcaacgc cctcctccga gatcttgcgc agctgcgcca gcgacatgac
     3001 ctgatatccg ccggaatcgg tgaggattgg ccccttccag cccatgaacc ggtgaagacc
     3061 gcccagacgc gcgatccgct ccgcacccgg gcgcaacatc aggtgatagg tgttggcgag
     3121 cacgatatct gcgcccgttg aacgaacggc ttcgggaagc atggccttca cgctggctgc
     3181 agttcccacc ggcatgaagg cgggggtggc gatgtcgccg cgcggtgtgt ggagaatggc
     3241 cagccgggcc gcgccgcagg ttgcctgtac ctcgaagcgc agcccgctca tgctgtcgtc
     3301 cgaaacagca ggcacgcatc cccgtaggag tagaagcgat acccatcctc aatcgcgagg
     3361 gcgtacgcgc gcttcatcct ctctatgcct gaaaacgcgc aggcgagcat gaacagcgtc
     3421 gagcgcggca gatgaaaatt cgtcagcaga atgtcgacag ccttgaagca atacccgggt
     3481 gttacaaaga ggtccgtttc gccttcgaat ccacgaacgg cgccgtcatc gcttgccgag
     3541 gtctccagcg tccgcaacga cgtcgtgccc acggcaatga tacgcccccc ggcctgccgg
     3601 gcggcacaaa tccgtgcagc ggactgcgcg ctcaggcgcg tccattcgcc gtgcatgcgg
     3661 tggcaggcgg tgtcgtccgc aataaccggg agaaacgttc ccgcgccaac atgcagcgtg
     3721 atgcgttcaa cgccaatccc ggctgaatcg agcgcagtca gcagcgccgg cgtgaaatgc
     3781 agacctgccg tcggcgcagc aacggagcct tcgtgagacg cgaacatcgt ctggtagtcg
     3841 atcacgtccc gcgcatcccc tgcccgccgg ctcgcgatgt agggcggcag aggcatttcg
     3901 ccatggcgcg agatcgcctg atcgagttcg gcgccggcaa tgtggaacgc aagttcgatc
     3961 tcgccgccgt cgcgtggtgc tgcaacgcgg gcggcgaatg ttccatgaaa atccagcgta
     4021 tcgcccgaag ccagccggcg cgctggtcgc gcgaaagcga gaaatcgatc cggcgcaacc
     4081 cgcttgtgca acaggatctc gatcgccgcg ccctctcccg cgccgcgcgc cgcccttcgc
     4141 gcccgcagcc gcgcgtgcac gaccttgctg tcattcacaa caaccagatc gccccgccgc
     4201 aggaaattcg gcaggtctcg aatccgggcg tgcgcgactt ctccatctgc acgcaccacc
     4261 atcattctgg cgctgtcgcg gggctccgcg gggcggagcg cgatacgatc cgccggcaat
     4321 tcaaagtcga acagggatac atccataaga gcgcgaatgt ccggaacggc ggctcaatgc
     4381 gtttgcccaa cgctttccag caccgcctcg ataatcatgt cttgagttgc ttcgtcgaga
     4441 tagggatgca tgggcaggct gatcacggtc cttccgagcg ccgtgctgac gggaagcggc
     4501 gccgaaggaa acgcgcgata tccgagttgc gccgagagtg gcgtcagata gtagatctgc
     4561 gccggaatgc cgcgagcgtc cagatcggct ttcagtctgt cgcgctcggg aacctgaatc
     4621 gtatattgcg cccatgtcga tcgcgcgcca tcgatgacac gagggatccg aatctgattc
     4681 gagcgcgtga aggcgttcga atagcggtgc gcgatacgtt cgcgcatctc gatctcctcc
     4741 ggaaatatct tgagcttctc gaggagtatc gcggcctgga tggtgtcgag ccttgagttc
     4801 acgccgatcc ggacgttgtc gtatttgtcg gcgccctggc cgtgaacgcg gatcgagcgc
     4861 aacagctcgg ccagtccatc gtcattcgtg aagctgcagc cgccgtcgcc atagcaacca
     4921 agtggtttgg cgggaaaaaa gctggtggag gacgcatcgc caatcgagcc cgtgcgcctg
     4981 ccgtcgagaa gggcgccgaa gccttgcgcg gtgtcgcaca gcatcaggag attcccgcgc
     5041 gccgcgatcg gctcgagcgc ccgataatcc gccgcctggc caaagagatc gaccggcatc
     5101 accacccgcg cacgcaaatc atcgtcccgc ctgaccattt cgatcgccgc gcagagactg
     5161 gccggcgcca tattgaaggt gtcctccagc acgtcgacga agacgggcgt tgctcccacg
     5221 agcgccacga cttccgcgga ggcagcaaac gtgaacgccg gcacaaacac cgcgtctccc
     5281 ggtcctacct cccaggccct gagaatgatg agaagcgcgt cggtgccgtt cgcgcaggcg
     5341 atcgcgtgtt tcacgccggc gaagtgggcg agctgctctt cgagtgcgcg cacctgcgga
     5401 cccatgatcc actggccgct ctcgaccgct gcggtgatgg cccgctccag cggcgctccg
     5461 agtcggcggc gctgcgcctg aaggtcgatg aatggaatcg agggcatgtg ccggcactcc
     5521 gcttctgttc tgcggcggtt ctagaaaacg gcgcctgtag cggcaatcgc gacaatgaca
     5581 cctaatattg cgaaatgcgt catcggctga agctgcggcg gttcaacttt ggcctggtcc
     5641 gacccgcatg gttacgatgc ggtccggctc accggcgatt gcgccattgt tgtgcttgcc
     5701 gcctttcttg atcgcatcga cgtgctccat gccgtccacc acctggccaa ataccgtgta
     5761 ctgacgatcg agccaggggc agtcggccag gcagatgaag aactggctgt tagcgctgtc
     5821 cggatcggcg gcgcgcgcca tcgagagtgt gcccctcaga tgacgttccg gcgaaaattc
     5881 cgccttcagc ctcggcaagt gcgagccgcc cgatccggtt ccggtcggat cgccggtctg
     5941 cgccatgaac tccgggatga cgcggtggaa cgcgtggccg tcgtagaatt ttgcgccggc
     6001 gagttccttg atacgcgcga catgcagagg cgcgagatcg ggcctgagcg ctattctcac
     6061 ctcgccggtt ttcaacgtca gcaccaccat gttgtcaggg tccgtcacgg ggttttcctt
     6121 gcttgtgccc gctccgcaag gagctgggcg acgtggggcg tcgtgaaggg cgcaacatta
     6181 ccccccagga tcgcgatttc cttgacgagc ttcgaggcaa tcgcctggtg gcgtggatca
     6241 gccatgagga acactgtctc gatatcggga ttgagccgct ggttcatcgc caccatctga
     6301 aactcgtatt cgaaatcgga caccgcgcgc aggccgcgca cgatcaccga tgcgccgacc
     6361 ttttcggcaa actgcatgag gagcgaatcg aaagtctcca cgctgatggt cgcgcgtccg
     6421 ctgccgccaa gcggcgccgt ctcgctgcgc accatgtcca cgcgctcggc cagcgtgaac
     6481 atcggggatt tggacgggtt cgtcgccacg ccgatgacga gatgatcgac gagcttgacg
     6541 gcgcgctgga tgatgtcgag atgcccgagc gtgacgggat cgaaggtgcc cggataaagc
     6601 ccaacgtggc gcctggtcat gcttgtgtcc ccttcacgcg cccgcttcgg ccgacgtttc
     6661 ctcaatacgc tccaccgaga ccacccgctc gcccgtctcg agccggaaca gggttacgcc
     6721 ctgcgtggcg cgtccggcga cgcgcacact cgcgaccggg ctgcggatcg tctggccctg
     6781 gtcggtgatg agcatgatgt cgtcgctttc ctcgaccgga aatgccgcca cgatggcgtt
     6841 gtttttgcgc gccatcgcta ttgcaactat gcccgagccg ccacggcccg tcacgcggta
     6901 ttcaaaggaa ctggtgcgtt tgccaaatcc ggttgccgaa accgcaagca cgaactgctc
     6961 cgccgcggcg aactgcgcat agcgttcggc ggtgaggatc gcttcctcgc cgccttcttc
     7021 ggactcttcg gaaacttctt cgctttcttc tccggtagct gcgcgccgca cggcacccgc
     7081 ctgcttgaga taggcccgtg tctcaggaat ttcggcttcg acatggtgaa gcagcgcgag
     7141 actgaccact tcgtcgcccg atgccagctt gatccccctg acgcccgtcg attgacggcc
     7201 cttgaatatc cgcacgtcgg tgagcgcgaa gcggatgcat ttgccggctc tcgtcgtcag
     7261 caacaagtcg tccgcatcgg tgcaggtctg cacgcccaca attccgtcgc cagactcaag
     7321 cttcatcgcg atcttcccgt tcgccttgac gttgacgaag tccgacagtt cgtttcggcg
     7381 cacatcgccc aatcgcgtcg cgaagaccac gttgagtttc tcccattgcg cctcatcctc
     7441 gggcaacggc agaactgtgg tgatgcgttc tccctcggca agcggcaaga gattgaccat
     7501 ggccttgccg cgcccctgaa tgcgcgcttc cggcaggcgc cagaccttga gcttgtaggc
     7561 catgccggtc gacgaaaaga acagaagcgg cgcgtgggtg gaggcgacga agatgtgggt
     7621 gacaaaatcc tcttcccgcg tcgccattcc ggtgcgaccc ttgccgccgc gtccctgcac
     7681 gcgatattcg gcgagcggcg tgcgcttgat atagcccgca tgggtgaccg tgacggccac
     7741 gtcctcgcgt tcgatcagcg cctcgtcttc gatctctacc tccgcgtcga tgagagcggt
     7801 gacgcggggc gtcgcgaaat cctttcttac ctcctcaagc tcggcgcgca cgatggccag
     7861 aacccgcggc cggctgcgca ggatgtcgag gtagtccttg attgcttcgc ccagtttcgt
     7921 cacctcctca gccaactcgt tgcggccaag ggcggtaagc ctttggaggc gaatttccag
     7981 aatggccttc gcctgttcgt cggtcagccg aatcgtgccg tcgggttgaa ccacatgcct
     8041 cggatcggca accagcgcca cgaggggcgc cacgtctttg gcgggccacc cacgctccat
     8101 caattgtatc tttgcggttg cggcgtcggc ggcgccacga atcagcgcaa tgacctcatc
     8161 gatgttggcg acagcgattg ccagtccgac gaggacgtgg gcgcgctccc gcgccttggc
     8221 aagttcgtgg cgcgtacggc gggtgatcac ttcctcgcgg aagcgcagga aggccgcaat
     8281 gaggcctttg agattcatca gctcgggccg cccgccattg agcgcgagca tgttgacgtt
     8341 gaaggacgtc tgcagctgcg agtggcgata gagctggttg agcacgacct cggatgatgc
     8401 gtcgcggcgc agttcgatga cgacgcgcat gccgtgacgg tcgctttcat cgcgcatgtc
     8461 ggcgatgccc tcgacgcgct tgtcgcgcac cagctccgag atgcgctcga tcatcgtcgc
     8521 cttgttcacc tgatagggga tctcggtgac gatgatggct tcgcggtcct tgcgcagttc
     8581 ttcgatgtgg attttcgccc gcatcaggat cgggccgcgc ccccgcgcca gcgccccgcg
     8641 cgcggccagc ctgccgatga tcagggcgcc ggtgggaaaa tcggggccgg gcacgatctc
     8701 ggtgagggcg tccaggctga tcgagggatc gtcgagccag gcgaggcagg catcgatcac
     8761 ttcgcccaga ttgtgcggcg gaatgttggt cgccattccc acagcaattc cggtgccgcc
     8821 attgacgaga aggttgggaa accgcgccgg cagaacgacg ggttcgcgct ccgtcgcatc
     8881 gtaattgtcc tgaaactcga cggtgtcttt ctcgaggtcc tcggtcagcg catgggcgat
     8941 acgagcgcgc cggacctcgg tgtaccgcat cgccgcgggc ggatcgccgt ccaccgagcc
     9001 gaaattgccc tgcccgtcga tgagcggcac gcgcatcgag aattcctgcg ccatgcgaac
     9061 cagcgcgtcg tagacggatt ggtcgccatg ggggtgatat ttgccgatga cgtcgccgac
     9121 gatccgcgcc gatttgcggt atggcttgtt ccagtcattg ccgttctcgt gcatcgaaaa
     9181 gaggatgcgc cgatgcacgg gcttgagccc gtcgcgggcg tcggggagag cccgcgcgac
     9241 aatcacgctc attgcgtaat cgaggtagga gcgtttcagc tcatcctcga tcgcaatatg
     9301 gcttatgcct gcgcccggcg gcccgccgat cgccgcgata tcgctcaaat tccgctcctc
     9361 aaggggccaa ttcaggccgc cgtatcaggg cccgaaggtg ggggcaaaac ctagcattcc
     9421 gcaccgcaaa tgtgccagcg aagaatgatc ctggcggtcc ctttcggaaa cctgaattat
     9481 ggcccttcag aacggaattt cgtcgtccat ttcgttccgc tcgaaactgg ccggcgagtc
     9541 gccggcgctc gcgctgtggg acggcgcggc cgccgcgccg ccgccgctgt cggcgcgcga
     9601 atcgagcatg gtgatttcgc cgcgaaagcg cggaacgacc acctcagtgg tatatttctc
     9661 cgcgccgtcc ttgtcggtcc attttcgggt ctgaatctgc ccttcgagat agatcttgga
     9721 gcccttgcgc agatattgct cggcgatctt tgcaaggtgc tcgttgaaga tcacgacccg
     9781 atgccattcc gtccgttccc ggcgctcgcc actctgctt
//
LOCUS       DASZMC010000168         3256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1984114,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000168 DASZMC010000000
VERSION     DASZMC010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984114"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VG274_03350"
     CDS             complement(<1..1159)
                     /locus_tag="VG274_03350"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HWE05716.1"
                     /translation="MEQEVYRHVPYDIDVEQALLGAIFVDNRAVERVSMLLRSEQFYD
                     PLHQRLFEGIVTSFERGGIVITPLTLHAQMKADPGLLEVGGLSYLANLAAAAPALPNV
                     RDYARILHDLWVRRALIHIGEDIVNTAYEAPLEKPPQAQIAEAEKALYGISETSRYGD
                     GPLDFAEALRRTVEVAEKAQQRGGRISGVSSGFADIDSLLGGLQPSDLLILAGRPGMG
                     KTALATNIAFNAARFHLQDEADGAPVSRGAPVLFFSLEMAAQQLSARILSEQTEIEMS
                     KIRSGRFAESEWERFVLKMQDLSTLPLYIDDSGGISIAQIAARARRFRREKQIGLVVI
                     DYLQLIEPTRRSDSRVQEVTEVTKGLKALAKELNVPVLACSQLSRGVDSRDD"
     gene            complement(1269..2513)
                     /locus_tag="VG274_03355"
     CDS             complement(1269..2513)
                     /locus_tag="VG274_03355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HWE05717.1"
                     /translation="MLGRLLASIVKKGSLTLIAPGGGPRTFGSGAPEVAMRLHDRRAA
                     VELALNPELKLGELYMDGRLTVENGDIADLLDLLMHNLSLASPRGVLKAERVWRWVTR
                     RFAQLNYSSRARRNVAHHYDLSGKLYDLFLDRDRQYSCAYFSAPGETLEEAQVGKKRH
                     IAAKLHLDTPDLRVLDIGSGWGGLALDLARDCGAKVLGVTLSQEQIALARSRAQNAGL
                     AERCRFELVDYRALSGTFDRIVSVGMFEHVGVPYYRAFFDKLRALLADDGVALLHTIG
                     RSDGPGVTSPWTAKYIFPGGYTPALSEVLPAIERAGLIVTDIEVLRLHYAETLKEWRR
                     RFKAHRSAVAELYDERFCRMWEFYLAGAEMAFRHDGQVVYQIQLTKRIDTLPLTRDYM
                     FESERTMRFAGTEAMPRQVRAA"
     gene            2690..>3256
                     /locus_tag="VG274_03360"
     CDS             2690..>3256
                     /locus_tag="VG274_03360"
                     /inference="COORDINATES: protein motif:HMM:NF021829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWE05718.1"
                     /translation="MVPKKASWTAELLRLAIRLFLKPGMHPGISIAARRQRTSACERW
                     VKWTPSGTVTADLALGGVPAVRVARPESQPGRHILFLHGGGYISGSAALYRHITWRLA
                     AAAQAQLIAIDYRLAPEHPFPAALDDATEAWYALLAEGADPERCSIMGDSAGGGLALA
                     LALRLRDEGGPLPAAVVALSPWTDLAITG"
BASE COUNT          600 a         1010 c         1056 g          590 t
ORIGIN      
        1 tgtcgtcgcg gctgtcgacg ccgcgtgaga gttgcgaaca ggcgagaacc ggtacgttca
       61 gctccttggc gagcgccttg agccctttcg ttacttcggt gacctcctgc accctgctgt
      121 cggagcggcg cgtcggttcg atgagctgga gatagtcgat aacgacgagg ccgatctgct
      181 tctcgcggcg gaagcggcgg gcgcgggcgg cgatctgcgc aatcgaaatg ccgcccgaat
      241 cgtcgatata gagcggcagc gtgctcagat cctgcatctt cagcacgaag cgctcccatt
      301 cggattcggc gaaccggccg gagcggatct tcgacatttc gatctcggtc tgctcggaga
      361 gtatgcgtgc ggagagctgt tgcgccgcca tttcgagcga aaagaagagc accggcgcgc
      421 cgcgcgagac cggggcgccg tccgcctcat cctggaggtg aaaacgcgcc gcgttgaacg
      481 cgatgttcgt cgccagtgcc gtcttcccca tgccggggcg ccccgcaagt atcagaagat
      541 ccgaaggctg caatccgccc agcagggaat caatgtctgc aaaaccgctc gagacgcccg
      601 aaatgcgtcc accgcgttgc tgcgccttct cggcgacctc gacggtgcgc cgcaacgcct
      661 cggcgaaatc gagcggaccg tcgccgtaac gggatgtttc cgaaatcccg tagagcgcct
      721 tctcggcttc cgcaatctgc gcctgcggcg gtttttcgag cggcgcctca taggccgtat
      781 tgacgatatc ctcgccgata tggatcagcg cccgccgcac ccagagatcg tgcaggatac
      841 gtgcgtaatc gcggacattg ggaagcgcgg gcgcggccgc cgcgagattg gcgagatagg
      901 aaaggccgcc cacttcgagc agccccggat cggccttcat ctgcgcgtgc agcgtcagcg
      961 gcgtaatcac gatcccgcca cgctcgaagc tcgttacaat tccttcgaac aggcgctggt
     1021 gcagcgggtc gtagaactgc tcggacctga gcagcatgga gacgcgctcg accgcccggt
     1081 tgtccacgaa aatcgcgcca agcagagcct gctcgacgtc gatgtcatag gggacgtggc
     1141 gatagacctc ttgttccatt ccgcgggcgc cagatttcgc aagtggaggg aaggcgaatc
     1201 aaacgagagc gcggggcttg cgcaaggccg gccgcgtcat tgcccacagc taaattgtcg
     1261 atatgccgct atgccgcgcg gacctggcgt ggcatcgcct cggttcccgc aaagcgcatg
     1321 gtgcgctcgg attcgaacat gtaatcgcgg gtcagcggta gcgtgtcgat gcgcttggtc
     1381 agctgaatct ggtagacgac ctggccgtcg tggcgaaacg ccatttctgc acccgcaaga
     1441 taaaactccc acatgcggca gaagcgctcg tcgtaaagtt ccgccactgc gcttcggtgc
     1501 gccttgaagc ggcggcgcca ttccttcagc gtctcggcat agtgcagcct gagcacttcg
     1561 atgtcggtga cgatcagtcc cgcgcgctcg atcgccggca gcacctcgct gagcgcaggc
     1621 gtatatccgc cgggaaaaat gtacttcgcc gtccacgggc tcgtgacgcc cggcccgtcg
     1681 ctgcggccga tcgtatgcag gagtgcgaca ccgtcatcgg cgagcagcgc gcgcagcttg
     1741 tcgaagaagg cgcgataata gggcacgccg acgtgctcga acatcccgac cgacacgatg
     1801 cggtcgaatg taccgctgag cgcccggtag tccacaagct cgaatcggca gcgttccgca
     1861 aggccggcat tctgggcccg gctccttgcg agcgcgatct gctcttgcga aagagtcacg
     1921 cccagcactt tggcgccgca gtctcgcgcg agatcgagcg ccagcccgcc ccagcccgaa
     1981 ccgatgtcga ggacccggag gtccggtgtg tcgaggtgga gcttggcggc aatgtgccgt
     2041 ttcttgccga cttgcgcttc ttcgagcgtt tcaccgggcg cggaaaaata tgcgcaggaa
     2101 tattgccggt cgcgatcgag gaaaagatcg tagagctttc ccgaaagatc gtaatgatgg
     2161 gcgacgttcc tgcgcgcgcg cgaggaataa ttgagttggg caaaccggcg ggtgacccag
     2221 cgccagactc tttcagcctt caggacgccg cgtggcgagg cgagcgagag attgtgcatc
     2281 aaaaggtcga gaaggtcggc gatgtcgccg ttttccacgg tgagccggcc atccatgtag
     2341 agctcgccca gtttcaactc gggattgagt gcgagttcca cggccgcgcg gcggtcatgc
     2401 aggcgcatcg ccacttccgg cgcaccgctg ccgaacgttc tgggtccgcc gcctggcgca
     2461 atcagcgtca ggctgccttt tttcacgatt gatgcgagga gccgaccgag cattccaagc
     2521 ttcccccttc cgcacaacct agcgtaccca atgaaagcgg ggaaagaaaa ttggttccag
     2581 cgggcagcga atacgtcgcc ccggaggggc ctggggaccg aatacggctt tgtaactggc
     2641 caatgctggt ccgatcccgt aagggctagc cggtcggagg gacaagtgaa tggtaccgaa
     2701 aaaggccagc tggacggccg agctgctgcg gcttgcgatt cgtctctttc tgaaacctgg
     2761 catgcatccc ggaatcagca ttgcggcgcg ccggcaaagg accagtgcct gcgagcgctg
     2821 ggttaagtgg acgccttcgg gaacggtaac agcggatttg gcgctgggcg gcgttccggc
     2881 ggttcgcgtg gcgcggcccg aatcacaacc cggccgccat atcctgtttc tgcatggcgg
     2941 cggctacatc agcggctcgg ctgcgttata ccgccacatt acctggcgct tggcggcggc
     3001 ggcccaggca cagctcatcg ccatcgatta tcggctggca ccggagcatc cgttcccggc
     3061 ggcgctcgat gacgcgaccg aggcttggta tgcgctgctt gccgaaggcg ccgacccgga
     3121 aagatgctcg atcatgggag actctgcggg aggaggtctc gcgctcgcgc tggcgctcag
     3181 actgcgcgac gagggaggtc cgctgccggc ggccgtggtc gcgctttcac catggactga
     3241 tctcgcaatc accggc
//
LOCUS       DASZMC010000169         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_352721,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000169 DASZMC010000000
VERSION     DASZMC010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352721"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="plsX"
                     /locus_tag="VG274_03365"
     CDS             complement(<1..1009)
                     /gene="plsX"
                     /locus_tag="VG274_03365"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWE05719.1"
                     /translation="MSIDAMGSDAGPGMVVAALARTVLRHPAARFILHGDETALKALL
                     AKRAKLAERVTIRHTAEEVRMDDKPSLVLRRGRNTSMWRAIESVKNKEAEVAISAGNT
                     GALMAMSMFQLGVLEGISRPAIAALWPTKRGQSVVLDVGANISCDAEQLVDFAVMGEA
                     FARAILGLERPTVGLLNVGSEDLKGHDAVKGAALILRNASLPMEYIGFVEGDDISDGT
                     ADVVVTDGFTGNIALKTAEGTAKMVIGFLRQALRGSLFGRIGGLIASGALKALRRKLD
                     PNSSNGGIFLGLNGIVVKSHGGADAMAFAGALDMAIDMADAGIIARIVADRANMLPPA
                     PE"
     gene            complement(1068..1247)
                     /gene="rpmF"
                     /locus_tag="VG274_03370"
     CDS             complement(1068..1247)
                     /gene="rpmF"
                     /locus_tag="VG274_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWE05720.1"
                     /translation="MAVPKRKTSPSKRNMRRSHHALAPAAHAECPNCGEQKRPHHVCA
                     KCGHYNEREVTKAKA"
     gene            complement(1400..>1581)
                     /locus_tag="VG274_03375"
     CDS             complement(1400..>1581)
                     /locus_tag="VG274_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05721.1"
                     /translation="GREEIESLRYDLAVPVLEEFALAIDPYPRAPGVEFDPPADEADS
                     PIHPFAALKGLKNQS"
BASE COUNT          299 a          519 c          469 g          294 t
ORIGIN      
        1 cctcgggcgc tggcggaagc atattggcgc gatctgcaac gatgcgcgca ataatgcccg
       61 catcggccat atcgatcgcc atgtccagcg caccggcaaa cgccatcgcg tcggcgcccc
      121 catggctctt caccacgatt ccgttcaggc ccaaaaagat accgccgttg gacgagttgg
      181 gatcgagctt gcggcgcagc gctttcaacg cgccgctcgc gataagccca ccgatgcgac
      241 cgaacagcga accgcgcagc gcctggcgca ggaaaccgat gaccatcttg gcggtgccct
      301 ccgccgtctt gagcgcgata ttgccggtga acccgtccgt gaccacgaca tccgctgtgc
      361 cgtcggaaat atcgtcgcct tcgacaaacc cgatatactc cataggcagc gacgcgttac
      421 gcagaataag cgcggcgcct ttcacggcgt cgtgaccttt cagatcttcc gatccgacat
      481 tgagcaaccc aacggtcgga cgttcgagcc caagaatagc acgggcgaac gcctcgccca
      541 tgacggcgaa atcgacgagt tgctccgcgt cgcaggaaat gttcgcgccg acgtcgagca
      601 cgacgctctg accgcgcttt gtcggccaca acgcggcgat cgcaggacgc gaaatgcctt
      661 cgagcacgcc gagttgaaac atcgacatcg ccatcagcgc gccagtgttg ccggccgaga
      721 tcgccacttc cgcttccttg ttcttcacgg attcaatcgc gcgccacatg ctggtgttgc
      781 ggccgcggcg cagcacgagg cttggcttgt cgtccatacg cacttcttcc gcggtatgtc
      841 ggatcgtgac gcgctcggcg agtttggcgc gcttcgccag cagcgccttt agcgccgtct
      901 catcgccatg caggatgaat ctcgcggccg gatgccgcag caccgtacgc gcgagcgcag
      961 cgacgaccat gccggggccg gcgtcgcttc ccatcgcatc gatagacact gtcagttcgc
     1021 gggccaattc gctcgagcct ttaagagacc gcaggccgcc gattatgtca ggcttttgcc
     1081 ttcgtcactt cacgttcgtt gtagtgaccg cattttgcac agacatggtg cgggcgtttc
     1141 tgctcgccac aattcgggca ctcggcatgg gctgccggag ccagagcgtg gtgcgaacgg
     1201 cgcatgttcc gcttcgacgg cgacgttttt cgtttaggca ccgccattgc ggaaactcct
     1261 taggaccccg ggacggaatc ggcacagggc gcatcgttgc gacgcgctcg gccatccggc
     1321 ggggcgggta aaacaaggcg cggagtgaac aggaaagcgt cgcaggattc aagccgcaaa
     1381 aaccccggtt ttctccggtt taggactggt ttttgagacc tttcagcgca gcaaaggggt
     1441 gaataggcga atccgcctca tccgcaggcg gatcgaactc cacccccggc gcccgcggat
     1501 acgggtcgat ggcgagcgcg aactcctcca gcaccggcac cgcgaggtcg tagcggaggc
     1561 tctcgatttc ctccctccca t
//
LOCUS       DASZMC010000170         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2269752,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000170 DASZMC010000000
VERSION     DASZMC010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269752"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VG274_03380"
     CDS             <1..305
                     /locus_tag="VG274_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HWE05722.1"
                     /translation="AAASGGAQADAWRKTAKDESAKQTKDRKKATENESDRDRLIAES
                     RTHEGRHHWLTGAATLFEIGIAMSTVAIITRRRWLSFGATAFGVGGILLLGVTYVV"
     gene            complement(383..1474)
                     /gene="rmuC"
                     /locus_tag="VG274_03385"
     CDS             complement(383..1474)
                     /gene="rmuC"
                     /locus_tag="VG274_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWE05723.1"
                     /translation="MASSLVVLSAALLLAAVAVFALVFVATRRGRGGDEALSPRMEAL
                     AAAQSEIAGRFAQALAGQTELQTVLAGRLDALDRRLGDSLKETASRTAETLGGIQTRL
                     SVIDEAQKNIAALSGHVVSLQEILANKQARGAFGQQQMEAIIADALPPSLYTFQATLS
                     NGSRPDCLIRLPNAAAAIIIDSKFPLESIAALRAAEESERKSALVAVRADMLRHVKDI
                     ASKYLIPGETQTPAIMFVPSESIYADLHEFCPEVIQKALREQVVVVSPSILMLAIVTV
                     QTVLKDARMREQASLIQKEVGLMMQDVKRLVERVGALTRHFTQSESDLREIAVSTEKI
                     VRRAGSIEAVDLAPTDVARLPDEAMSPFP"
     gene            1532..2050
                     /gene="def"
                     /locus_tag="VG274_03390"
     CDS             1532..2050
                     /gene="def"
                     /locus_tag="VG274_03390"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWE05724.1"
                     /translation="MPIRPILIAPDPRLKAVSLTVEAVDSGIRRLADDMFESMYAAEG
                     IGLAAIQIGVPKRLLVMDLAQKDGRREPRVFINPKILWTSDETTICEEGCLSVPDIWE
                     EVERPAQIRAEYLDRDGTRHTLEAEGLLATCLQHEIDHLEGVLFVDHLSRLKRSMALK
                     RLAKAQRLKQAG"
     gene            2060..2974
                     /gene="fmt"
                     /locus_tag="VG274_03395"
     CDS             2060..2974
                     /gene="fmt"
                     /locus_tag="VG274_03395"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWE05725.1"
                     /translation="MGLRLVFMGTPDFAVPTLAALIGHGHDIAAVYTKPSRPKGRGLV
                     AEMSPVAKLAAAHGVELRTPETLKDAPAEEAFAELGADAAVVVAYGLLLPKRVLDAPR
                     LGCFNLHASLLPRWRGAAPIQRAIMAGDAETGVMAMRMEEGLDTGPVLMAERVAIDRK
                     TYGELHDELAQLGADLIVRTLAALARGSISEHKQLEDGATYAKKISKDEGRIAWTRPA
                     RELDCLVRGLSPQPGAWCEARGERLKILYCDRMEGHGKPGQILDDRLTVACGDGAVRL
                     TCVQRAGRGAVDAAEFLRGFPLHRGEML"
     gene            2971..3342
                     /locus_tag="VG274_03400"
     CDS             2971..3342
                     /locus_tag="VG274_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05726.1"
                     /translation="MSGTLRPSLWRRMGNSWRARGRKPGAVDITPDGLVFTRGRNSKR
                     IRWEDVVRIDGGSRDSLSIDLFFVVIHARDTTMTIDEFDDGFRVLEYAILERWPQVRE
                     RFIALQCGAPRQPLYETLWQR"
     gene            3351..4097
                     /gene="truA"
                     /locus_tag="VG274_03405"
     CDS             3351..4097
                     /gene="truA"
                     /locus_tag="VG274_03405"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWE05727.1"
                     /translation="MPRYRLTLEYDGGPFAGWQRQENGPSVQAALERAIFSLSGEEVS
                     VTGAGRTDAGVHALGQVAHFDLEKTFAGDRLRDALNYHLRPDPIAVLGGQIAGSDFHA
                     RFSATARLYHYRIVTRRAPLALDRGRAWHIMRNLDADAMHEGARHLVGQHDFTTFRAA
                     ECQAQSPVKTLDSLSVRRAGEEIHIDAKARSFLHHQMRSIAGSLKVVGEGKWQPRDVA
                     NALAARDRSRCGPVAPPEGLYLVRVEYESR"
     gene            complement(4159..>5006)
                     /gene="dapE"
                     /locus_tag="VG274_03410"
     CDS             complement(4159..>5006)
                     /gene="dapE"
                     /locus_tag="VG274_03410"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF009557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HWE05728.1"
                     /translation="MKSAIAAFAAAAAQHSKAMSRGSISLLITGDEEGPAINGTAKVL
                     EWLAAKGERIDHCVVGEPTARTHAGDTIKIGRRGSLRAEITVRGVQGHTAYPQNALNP
                     IPVLAKIVCRLAGEPLDTGTAHFEPSTLVFTTFDVGNPASNVSPAVARAAASIRFNDL
                     QTPQMLASRIEERAAEATRAMGGEASVAIDVGALPFLTDPGAFTDLLARAIHSATGRA
                     PILSTTGGTSDARFIKNYCPVAEVGLAGATMHKTDECVPVAEIGNLTKIYTSILDAYF
                     AAPPE"
BASE COUNT          902 a         1597 c         1628 g          879 t
ORIGIN      
        1 tcgcggccgc ctctggcggt gcgcaggccg acgcctggcg caagaccgcg aaggacgaaa
       61 gcgccaaaca gacgaaagac cgcaagaaag cgacggaaaa cgagtccgac cgcgacaggc
      121 tgatcgccga aagccggacg catgagggcc gccatcattg gctgaccgga gcggccacct
      181 tgttcgagat cggcatcgcc atgtcgacgg tcgccatcat cacccgccgc cgctggctgt
      241 cgttcggcgc aacggcgttc ggcgtgggcg gcattctgct gctgggcgtt acgtatgtcg
      301 tgtagcgatc ccacctcccc ttgatggggg aggaaaggcg agcagccgac gaacgtgtca
      361 ccttatctta gcgcttatgc cgtcaaggga agggtgacat agcctcatcg ggcaatcgcg
      421 caacgtcagt tggcgcgaga tccaccgctt cgatcgagcc ggcgcgcctg acgatctttt
      481 ccgtcgagac cgcgatctcc ctcaaatccg actcactttg cgtgaaatgc cgcgtgagcg
      541 cgcccacgcg ctcgacgagg cgcttgacgt cctgcatcat caggcccact tccttctgga
      601 tcaggctcgc ctgctcgcgc atgcgcgcat ccttcagcac cgtctggacg gtgacgatgg
      661 cgagcatcag gatgctcgga gaaaccacga ccacctgctc gcgcaacgcc ttctgaatca
      721 cctccggaca gaattcgtgc agatcggcat agatggattc cgacggtaca aacatgatcg
      781 ccggcgtctg cgtttcgccg ggaatgagat atttcgaggc gatgtccttc acatgcctca
      841 gcatgtcggc gcgcaccgcc acgagagccg atttgcgctc cgactcttcg gccgcgcgaa
      901 gcgccgcgat gctctcgagc ggaaacttgg aatcgatgat gatggccgcc gcggcattcg
      961 gcagccggat caggcaatcg ggacggctac cgttggagag cgtcgcctgg aaggtgtaga
     1021 gcgacggcgg cagcgcgtcg gcgatgatcg cttccatctg ctgctggccg aatgcgccgc
     1081 gcgcctgttt gttggcgaga atttcctgca gacttacgac gtggccggac agcgcagcga
     1141 tgttcttctg cgcttcgtcg atgacgctta gccttgtctg aatcccaccc agcgtttcgg
     1201 ccgttcggct cgcggtctcc ttgagactgt cgccgagccg ccggtcgagc gcgtcaagcc
     1261 gccccgccag cacggtctga agctccgttt gccccgccag cgcctgggcg aatcggccgg
     1321 cgatctcgct ctgcgcggcg gccagggcct ccatccgcgg ggaaagcgcc tcatcgccgc
     1381 cgcggccgcg ccgtgtcgcc acgaagacga gggcgaaaac agcaaccgcc gcgagcagca
     1441 gcgccgcaga aagaacaacg agggaagatg ccatttatga gcccacgctc cctgccttga
     1501 ccccgcaatg ggcctcgcct atttcccgtt catgcccatc cggcccatcc ttatcgcgcc
     1561 cgatccccgc ctgaaagccg tctcgctgac ggtggaagcg gtcgattcgg gaattcgccg
     1621 gctggccgac gacatgttcg agagcatgta tgccgccgag ggaatcgggc tcgccgcgat
     1681 ccagatcggg gttcccaaac gcctgctcgt catggacctc gcgcagaagg atggccgccg
     1741 cgaaccgcgc gtgttcatca atccgaaaat tctctggacg tcggacgaga ccacgatttg
     1801 cgaggagggc tgtctctctg tccccgatat ctgggaagag gtcgagcggc cggcgcaaat
     1861 ccgcgccgaa tatctcgatc gcgatggcac gcggcacacg ctcgaagccg aaggcctgct
     1921 cgcgacctgc ctgcagcacg agatcgacca tctcgaaggc gtgctctttg tcgatcatct
     1981 gtccaggctc aagcgctcca tggcgctgaa gcggctcgcc aaggcccagc ggctgaaaca
     2041 ggcgggctga caggcgcgca tgggcttgcg cctcgtcttc atgggcacgc cggatttcgc
     2101 cgtgcccacg cttgccgcac tcatcggcca cggacacgac atcgccgccg tctatactaa
     2161 gcccagccgc ccgaaaggac gcggccttgt ggcggaaatg tcgcccgtcg caaagctggc
     2221 agcggcgcac ggcgtcgaat tgcgaacgcc cgaaacgctg aaagatgcgc cggccgagga
     2281 ggcgtttgca gagttgggtg cggatgcggc ggtcgtggtg gcctacgggc tccttttgcc
     2341 caaacgcgtt ctcgacgcgc cacggctcgg ctgcttcaat cttcacgctt cgcttttgcc
     2401 gcgctggcgc ggagcagcgc ccatccagcg cgcgatcatg gcgggcgatg ctgaaaccgg
     2461 cgtaatggcg atgcgcatgg aagaaggtct cgatacgggc cctgttctga tggcggagcg
     2521 cgtggcgatc gaccgcaaaa cttatggcga actgcacgac gaactggcgc agctcggcgc
     2581 agatttgatt gtccgcacgc tggccgcgct tgcgcgcgga agcatcagcg agcacaagca
     2641 gctcgaagac ggcgcgactt atgcgaagaa gatctccaag gacgaagggc ggatcgcctg
     2701 gacgagaccg gcccgggaac tcgactgtct ggtccgcggg ctttcgccac aaccgggcgc
     2761 ctggtgcgaa gcacgaggcg aaaggctgaa gatcctctat tgtgaccgga tggaaggtca
     2821 cggaaaaccg gggcagattc tcgacgaccg cttgacggtc gcctgtgggg acggcgccgt
     2881 gagacttaca tgcgtgcagc gcgcgggccg cggcgccgtg gacgccgccg aattcctgcg
     2941 cggcttcccg ctgcacaggg gagaaatgtt gtgagcggaa ccctgcggcc gtcgctgtgg
     3001 cggcggatgg ggaattcctg gcgcgcacgc ggcaggaagc ccggggccgt agatatcacg
     3061 ccggatggat tggtctttac caggggccgc aattcgaagc gcatccgctg ggaggacgtc
     3121 gtccggattg atggcgggtc gcgcgacagc ctttcgatcg atctcttctt cgtcgtgatc
     3181 cacgccaggg atacgacgat gacgatcgac gaattcgatg acggcttccg cgtgctggag
     3241 tacgcaatcc tcgaacgctg gccccaggtg cgcgagcgct tcatcgcgct ccaatgcggc
     3301 gcgccacgac aaccgctcta tgaaactctc tggcagcgat gacgggctga atgccccgtt
     3361 atcggctcac gctggaatat gacggcggcc cctttgcggg ctggcagagg caggagaacg
     3421 gtccgtcggt gcaggccgct ttggaacggg cgatattcag cctctccggc gaagaagtaa
     3481 gtgtgaccgg cgcgggccgc accgatgcgg gcgtgcacgc gctgggacag gtcgcgcatt
     3541 tcgatctcga aaagacgttt gccggagaca ggttgcgcga tgcgctgaac taccatctgc
     3601 ggccggatcc gatcgcggtg ctgggcgggc agattgcagg gtccgatttt cacgcgcgct
     3661 tttccgccac cgcgcgtctc tatcactacc ggatcgtcac cagacgggcg ccgcttgcgc
     3721 tcgatcgcgg ccgcgcctgg cacatcatgc gcaatctcga tgccgacgcg atgcatgagg
     3781 gcgcccgcca tctcgtgggg cagcacgatt tcaccacctt tcgcgccgcg gaatgccagg
     3841 cgcaatcgcc ggtgaagaca ctcgactcgc tcagcgtgcg ccgtgcgggc gaggagattc
     3901 acatcgacgc gaaagcgcgc tcgttcctgc accaccagat gcgctcgatc gccggctcgc
     3961 tgaaggtcgt cggcgaaggc aaatggcagc ctcgcgacgt cgcgaacgcg ctcgccgccc
     4021 gcgaccgctc acgttgcggc ccggtggcgc ctccagaagg gctttatctc gtgagagtcg
     4081 agtacgagag ccgttgaaac aaaacgtgtc attcccgcga aagcgggaat gacacgctat
     4141 tgttctccac gaatcgcatt actccggcgg cgcggcgaaa tacgcgtcca ggatggatgt
     4201 gtatatcttc gtgaggttac cgatctccgc gaccggcaca cattcatcgg tcttgtgcat
     4261 ggtcgcgccg gcgagaccca cttcggcgac gggacagtag ttcttgatga agcgcgcatc
     4321 cgatgtgccg ccggtggtcg aaagaatcgg cgcgcgaccc gtcgccgaat gaattgcgcg
     4381 cgcgagaaga tcggtgaacg cgccggggtc ggtcagaaag ggcagcgcgc cgacatcgat
     4441 ggcgacgctc gcttcgccgc ccattgcgcg cgtggcttcc gccgctctct cttcgatgcg
     4501 actcgcgagc atttgcggcg tctgcagatc gttgaagcgg atgctcgccg cagcgcgcgc
     4561 gacggcgggc gacacgttgg acgccggatt gccgacatcg aaagtcgtaa acacaagcgt
     4621 cgaaggctcg aaatgcgccg ttcccgtatc gagcggctcg cccgccaggc ggcagacgat
     4681 cttcgcgaga acgggtatcg gattgagcgc gttctgcggg taggcggtgt ggccctgcac
     4741 gccgcgcacc gtgatctcgg cgcgcaggct gccgcgtctt ccgatcttga tcgtatcgcc
     4801 ggcatgcgtg cgggccgtcg gctcgccgac gacacagtgg tcgatgcgct cgccttttgc
     4861 ggccaaccat tcgagcacct tcgcggtgcc gttgatcgcg gggccttcct cgtcgcccgt
     4921 gatgagcagg ctgatagagc cgcgcgacat cgctttggaa tgctgcgcgg ccgcagcggc
     4981 gaaagccgcg atcgcagatt tcatat
//
LOCUS       DASZMC010000171         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_889239,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000171 DASZMC010000000
VERSION     DASZMC010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889239"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..2432
                     /locus_tag="VG274_03415"
     CDS             162..2432
                     /locus_tag="VG274_03415"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HWE05729.1"
                     /translation="MATSNAIGPLGRAGLWQVIRAVSRPSRLAAGVGLLAVVSGVATY
                     ATVRGLVPYHPTSATLIVLLLLNLSLVLTLGALIVWRLTRLWIDRRSGIAGARLHVRL
                     VMLFSAIAVIPAILVAIFAVVTLDLGIEAWFSARVKSALGNAVHVAERYVQGNERLII
                     ADAYEIANTIEHDPELFDEHNRVRADILFTKLAILTKDRGLQAAYILDSHGHVLGSTR
                     QRFLPEIAPPKPGDIDQATHGMIVIDASSKVGVVRALIRMQALNDAYLLVVRTVDPQV
                     LGYYQRTVDAVSEYKRLDQNRSSVQLVFAGLYGAVSLLILLVAIWLGLWAANSLVLPV
                     SRLIRASERVSRGDLKAQVEIERSDDEMGMLARAFNRMTMELDTRRNELIHANQQLDE
                     RRRFTEAVLAGVSAGVIGLDGEGCVTIVNRAAARLINAEPEELEGRHYAEAVPELSAI
                     IRRALQEPLGHASGEATVRRGGTVRSLSVQVASEAGAGEAGFIVTFDDITDLVSAQRT
                     AAWADVARRIAHEIKNPLTPIQLSAERLKRKYAGEVSSDPEVFSQCTDTIIRQVGDIG
                     RMVDEFSSFARMPAPVMRRELAQELVQQAVFLQRAAHPQIDFEVAAGSPPIRFDCDGR
                     LVAQALTNVLKNASEAIEARTEAGEEERGRIAIAVAAERARVIFTVADNGIGLPHGQR
                     HRLTEPYVTTRAKGTGLGLAIVRKIVEEHGGEISLCDRENDEPGAEVRLIFPLKQKTA
                     RQTEAGDDKERIADRA"
     gene            2401..>2862
                     /locus_tag="VG274_03420"
     CDS             2401..>2862
                     /locus_tag="VG274_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE05730.1"
                     /translation="MTKNGSLIAPELLVVDDEEDIRELIAGILGDEGYTTRVAADSDS
                     ALAAVRQRRPQLVILDIWLQGSRLDGIQVLDVLKRDHPDLPVVIISGHGTIETAVASI
                     KKGAYDFVEKPFKADRLIHVAARALEAARLRREVQELKLKAGDDTDLVGASS"
BASE COUNT          490 a          944 c          946 g          482 t
ORIGIN      
        1 gcgcgcgcca cctccccaga agtggggagg aaagtctctg gcggcacttc cttccgcgtc
       61 tttccttccg cgtctttctt gcaacaggct gtggcttggt tacactggct ggaggagatg
      121 gggccggaat gtccggcggg cgggggcagc ggcaggcctg aatggcgaca tccaatgcga
      181 tcgggccgct ggggcgggcc ggtctgtggc aggtgattcg ggcggtctcc cggccctcgc
      241 ggctggcggc gggcgtgggc cttctcgcag tggtttccgg ggtcgcaacc tatgcgacgg
      301 tgaggggcct ggttccctac catccgacct ccgcgacgct gatcgtcctt ctgctcctca
      361 acctctcgct ggtgctgacg ctcggcgcgc tgatcgtctg gcggctgacg cggctgtgga
      421 tcgatcgccg ctccggcatc gccggcgcac ggctgcatgt gcgcctcgtc atgctgttca
      481 gcgcgatcgc ggtgatcccg gcgatcctgg tcgccatctt cgccgtggtc acgctcgatc
      541 tcggcatcga ggcgtggttc tcggcgcgag tgaagtcggc gctcggcaat gcggtccatg
      601 tcgcggaacg ctacgtgcag ggcaatgagc ggctgatcat cgccgacgcc tacgagatcg
      661 cgaacaccat cgaacacgat cccgagcttt tcgacgagca caaccgggtg cgcgccgaca
      721 ttctcttcac caagctcgcc attctcacca aggaccgcgg cctccaggcg gcctatattc
      781 tcgactcgca cggccatgtg ctcggcagca cgaggcagcg tttcctgccg gaaatcgcgc
      841 ccccgaagcc gggcgacatc gatcaggcca cgcacggcat gatcgttatc gacgccagtt
      901 caaaagtcgg cgttgtgcgc gcgctcatcc ggatgcaggc gcttaacgac gcctacctgc
      961 tcgtggtgcg caccgtcgat ccgcaggtgc tgggctatta ccagcgcacc gtcgacgcgg
     1021 tttcggaata caagcggctg gaccagaacc gctcctcggt gcagctcgtc tttgccggac
     1081 tctatggcgc cgtctctctt ctcatcctgc tcgtggcgat ctggctcggc ctgtgggcgg
     1141 caaacagcct ggtgcttccg gtgtcgcgac tcatccgcgc ctcggaacgc gtgtcgcgcg
     1201 gcgatctgaa ggcgcaggtc gagatcgagc gcagcgacga cgagatgggc atgctggcgc
     1261 gcgccttcaa tcgcatgacg atggagctcg acacgcggcg caacgaactg atccacgcca
     1321 accagcagct cgacgaacgc cggcgcttca ccgaggccgt gctcgcgggc gtcagcgccg
     1381 gtgtcatcgg gctcgacggc gaaggttgcg tcaccatcgt caatcgcgcc gcagcgcggc
     1441 ttatcaacgc ggagccggaa gaactggaag gccgccacta tgccgaagcg gtgcccgaac
     1501 tttccgcaat catccgccgc gcactgcagg agccgctcgg ccacgcgagc ggagaagcga
     1561 cggtccgccg cggcgggacc gtgcgcagcc tgagcgtgca ggtcgcgagc gaggcgggcg
     1621 cgggcgaagc gggcttcatc gtcaccttcg acgacatcac cgatctcgtt tccgcgcagc
     1681 gcacggcagc ctgggccgat gtggcgcggc gcatcgcgca cgagatcaag aatccgctga
     1741 cgccgatcca gctttcggca gagcgcctga aacgcaaata tgcgggcgaa gtgtcgagcg
     1801 atcccgaggt gttcagccaa tgcaccgata cgatcatccg ccaggtgggc gatatcgggc
     1861 gcatggtcga cgagttctcc tcctttgcgc gcatgcccgc gcccgtcatg cgccgcgaac
     1921 tcgcgcagga actggtgcaa caggcggtgt tcctacagcg tgccgcccat ccgcagatcg
     1981 atttcgaagt tgccgccgga tcgcccccga tccgattcga ttgcgacggc cgccttgtcg
     2041 cccaggcgct gaccaatgtg ctgaagaacg ccagcgaagc catcgaggcg cgcaccgaag
     2101 cgggtgagga ggagcgcggg cgcatcgcca tcgccgttgc ggcagagcgc gcgcgggtaa
     2161 ttttcaccgt cgccgacaac ggcatcggcc tgccgcacgg acagcggcac cgcctgaccg
     2221 agccttatgt gaccacgcgc gcgaaaggca ccggcctcgg cctcgccatc gtgcgcaaga
     2281 tcgtcgagga gcatggcggc gaaatctcgc tctgcgacag ggagaacgac gagccgggtg
     2341 cggaagtgcg cctgatcttc ccgctgaaac agaagaccgc acgccaaacg gaggctgggg
     2401 atgacaaaga acggatcgct gatcgcgcct gaactcctcg tcgtcgacga cgaagaggac
     2461 atccgtgaac tgatcgccgg gattctgggc gacgagggtt atacgacgcg ggtggccgcc
     2521 gacagcgatt ccgcgctcgc cgccgtgcgt cagcgccgtc cgcaactcgt catcctcgac
     2581 atctggcttc aggggagccg gctcgacggc atccaggttc tggatgtgct gaagcgcgat
     2641 catcccgacc ttcccgtggt catcatcagc gggcacggca cgatcgaaac cgccgtcgcg
     2701 tcgatcaaga agggtgccta tgatttcgtg gaaaagccgt tcaaggccga ccggctgatc
     2761 catgtcgcgg cgcgcgcgct cgaagcggcg cgactcaggc gcgaagtgca ggagctgaag
     2821 ctcaaggcgg gcgacgatac ggacctcgtc ggcgcatcct cc
//
LOCUS       DASZMC010000172         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5290510,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000172 DASZMC010000000
VERSION     DASZMC010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5290510"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..719
                     /locus_tag="VG274_03425"
     CDS             123..719
                     /locus_tag="VG274_03425"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWE05731.1"
                     /translation="MSRLDEEEKPKSPPPAEAQTSPVQQALFKARTVLIFGEIDMKLA
                     ERVTAQLLAHASEGDGDIRVVINSPGGHVESGDTIHDMIRFVGPSVKMIGTGWVASAG
                     AHIYIGAARENRFALPNTRFLLHQPLGGVAGPVSDISIEAQEILKMRERLNRTIAHET
                     GQSYEKVVTDTDRNFWMSAEEAMAYGLVARIVNRASEA"
     gene            716..2317
                     /locus_tag="VG274_03430"
     CDS             716..2317
                     /locus_tag="VG274_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWE05732.1"
                     /translation="MSEATPWWRGAVLYQIYVRSFADGNGDGIGDFAGLLSKLDYVAA
                     LGVDAVWLSPIHPTPNRDWGYDVSDYDRINPEFGNDADFESLLAQSHARGLKLLIDEV
                     LAHTSDEHPWFRASLEGGEQAGWYVWAPPREDGTAPNNWLSAFGGPAWSYHPARRQCF
                     HHKFLRQQPKLNWRNPGARAAALRVLDLWLDRGVDGFRLDVANAFVHDASLTDNPPVP
                     NELRTDLTWSQASYLQYHRHDSNLRENLEVLDEIRRRVERYRERFVMGEFSEEPDRSG
                     GLASSSQGLHSGYTFPLLKARNLGPAFVREHYEMLARYPDHWPSVAFSNHDVVRTVTR
                     FGGRDAPPALARLMLALLVALKGTPILYQGEELGLPETELRRDQLKDPVGDLYYPLAR
                     GRDGCRTPMPWDASALNLGFTTGTPWLPLSPSHRELAVSNQERDAGSTLSYARHVLAE
                     RKRIAALRLGEIQFLDASGPVLAFLRVHAVEQILCVFNMSNAEASFRDPRVVSGAPLA
                     LGCGAAIVSGSTLTIAPYAAWFARV"
BASE COUNT          439 a          759 c          715 g          459 t
ORIGIN      
        1 atctcgtttt ccagggcgaa atcggcccga cgctttccgc cgcggtagcc gcgctctgag
       61 tgggtggact tgagccggcc tcgccatata tgaccattgc cgtttctccc caggagcatc
      121 ccgtgtcccg ccttgatgaa gaagaaaaac ccaaatctcc ccctccggcc gaagcccaaa
      181 cgtcgccggt tcagcaagcc ctgttcaaag cacgaacggt gctcattttc ggcgagatcg
      241 acatgaaact cgcggagcgc gtgaccgcgc agcttctcgc ccacgcctcg gaaggggatg
      301 gagacatccg cgtcgtcatc aattcgccgg gcgggcatgt ggagtcgggt gatacgatcc
      361 acgacatgat ccgcttcgtc ggcccgagcg tgaagatgat cggcacggga tgggtcgcga
      421 gcgcgggggc gcacatctac atcggtgctg cacgcgagaa ccgcttcgcg ttgcccaaca
      481 cgcgattcct tttgcatcag ccgctgggcg gcgtcgccgg gcccgtctcg gacatttcca
      541 tcgaggcgca ggaaattctc aagatgcgcg agcgcctcaa ccgcacaatc gcgcacgaaa
      601 ccggtcaatc ctacgagaag gtcgtcaccg acaccgaccg gaatttctgg atgtcggcag
      661 aggaagccat ggcgtacggt cttgtcgcgc gcatcgtaaa ccgagcgagc gaagcgtgag
      721 cgaagccacg ccctggtggc gcggcgccgt cctctaccag atttacgtcc gcagtttcgc
      781 cgatgggaac ggagacggga tcggcgattt cgccgggctt ctgtcgaagc ttgattacgt
      841 cgcggcgctc ggcgtcgatg cagtgtggct ttcacccatt cacccgacgc cgaaccggga
      901 ttggggctac gacgtctccg attatgacag gatcaatccg gaattcggca acgatgccga
      961 tttcgaaagc ttgctcgccc aatcgcatgc acgcggattg aagcttctga tcgacgaagt
     1021 gcttgcgcac acctcggacg aacatccctg gtttcgcgcc agtctcgaag gcggcgagca
     1081 ggccggctgg tatgtttggg cgccgccgcg cgaagacggc acggcgccga acaattggct
     1141 gtcggctttc ggcggacctg cctggtcgta ccatcccgca cgccggcagt gctttcatca
     1201 caaattcctg cgccaacaac ccaagctcaa ttggcgcaac ccgggcgcgc gcgcggctgc
     1261 cctgcgtgtg ctcgacctat ggctcgatcg cggcgtcgac ggattccggc tcgatgttgc
     1321 aaatgccttt gtccacgacg cgtcgctgac cgataacccg cctgtaccca atgaactgcg
     1381 tacggatctg acctggagcc aagcctcgta cttgcaatac catcgccacg acagcaactt
     1441 acgggagaat cttgaggttc tggacgagat caggaggcgg gtcgagcggt atcgcgagcg
     1501 cttcgtgatg ggggaatttt cggaagaacc cgaccgcagc ggtggtctcg cgtcgtcctc
     1561 gcaagggctt cattccggat acacgtttcc gctgctcaag gcccggaacc tgggaccggc
     1621 gttcgtgcgc gagcactacg aaatgctcgc acgatatccc gatcattggc catcggtggc
     1681 attctccaac cacgacgtcg tgcgtaccgt gacgcgcttc ggcggcaggg acgcgccgcc
     1741 ggcgctcgcg cggctgatgc tggcgctgct tgtcgcgttg aaaggaacgc ccattctcta
     1801 tcagggcgag gagctcggcc tgccggaaac cgaattgcgg cgggatcagc tcaaggaccc
     1861 cgtgggcgat ctctactatc cgctcgctcg cgggcgcgat ggctgccgaa caccgatgcc
     1921 gtgggacgcg tcggccctca atcttggttt taccaccgga acgccctggc tgcccctctc
     1981 gccatcgcat cgcgaactcg ccgtctcgaa ccaggagcgc gacgccggct cgacgctctc
     2041 ctatgcgcgc catgtgcttg cggagcggaa acgcattgcc gcactgaggc tgggcgaaat
     2101 tcaattcctc gatgcatcag ggccggttct cgcatttttg cgcgtgcatg ccgttgagca
     2161 aattctctgc gtattcaaca tgagcaacgc cgaagcttcg tttcgggacc cgcgcgtcgt
     2221 gtccggcgca ccgctcgctc tgggctgcgg cgcggcaatc gtctctggca gcactctaac
     2281 catcgcgcct tatgcggcct ggttcgcccg cgtctaagcc gaatccacca aggtctcgtg
     2341 caaaaatagt ttgtgacgac ccggacgatt ta
//
LOCUS       DASZMC010000173         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3089684,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000173 DASZMC010000000
VERSION     DASZMC010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089684"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VG274_03435"
     CDS             complement(<1..1123)
                     /locus_tag="VG274_03435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF006585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWE05733.1"
                     /translation="MDDTQEPTLGFAPAPTKAEAYRVLARKYRPQDFTGLIGQQALVR
                     TLRNAFSTGRIAHAFMLTGVRGVGKTTTARIIARALNCVGPDGTRKEPTIQPCGVCEP
                     CVAIAESRHLDVQEMDGASNNGIEEVRQIIDSVRYAPASARYKVYIIDEVHMLSKQAF
                     NALLKTLEEPPAHVEFIFATTEIRKVPVTILSRCQRFDLRRIETGELVSHLSNIAAKE
                     NVEIEPAALSLIARAAEGSVRDALSLLDQAIAHEEGHAIGAGSVRAMLGLADRGRILD
                     LFEKTMRGEIAAALADFGDIYAGGADPLVVVQDLLEITHYLTRLKVAPAAEGFFDGGS
                     GEARRATEMAAKLPLSALARAWQMLLKGLIEVRDAVRPIA"
     gene            complement(1255..1345)
                     /gene="ffs"
                     /locus_tag="VG274_03440"
     ncRNA           complement(1255..1345)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VG274_03440"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1809..2513)
                     /locus_tag="VG274_03445"
     CDS             complement(1809..2513)
                     /locus_tag="VG274_03445"
                     /inference="COORDINATES: protein motif:HMM:NF045345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05734.1"
                     /translation="MSTIQSRADTNLIGKGLYTVPEASRITGVPAETFRRWVFGYARR
                     RNCARVEYAPLTAPEIGRIEGQCIVGFRDLLEARVVNAFREAGVSWHVIRQAARNTQT
                     IDRPWHPFLSRRFRTDGRTIFLEIAKQSDEPKLIDLARNQLAFHSVIAPSLFKQIVFG
                     DDDEPKLWYPAWPRKTIVIDPQRAFGRPLSGDVPADSLAAAACAENSAEIAACWYGVP
                     KEAVDAAVEWEKRLAA"
     gene            complement(2678..3418)
                     /locus_tag="VG274_03450"
     CDS             complement(2678..3418)
                     /locus_tag="VG274_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWE05735.1"
                     /translation="MIPPAVILSHPQLGENIGAAARAMANFGLSDLRLVAPRAGWPNA
                     KAAAMAAGAANVVDGALVFATVAEAIGELNLVFATTARERGIAREVLTPAEAARRLRV
                     ASMRGERTGILFGNERAGLDNDEISLCDAVITVPTAEFASLNLGQAVLVTSYEWFRAG
                     DATPPARLEHGPLQRKPTREEMLQLFEHLERELLASGFLYPPDKVDGMARAIRATLHR
                     ARLTYQEVQTLRGMIVALAKGKHRVRRS"
     gene            3500..>4165
                     /locus_tag="VG274_03455"
     CDS             3500..>4165
                     /locus_tag="VG274_03455"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWE05736.1"
                     /translation="MNKIKVANPVVELDGDEMTRIIWELIKRKLILPYLDIQLEYFDL
                     GIGHRDATGDQVTIDAAEAIKRTGVGVKCATITPDEARVAEFKLGKMWKSPNGTIRNI
                     LGGVIFREPIICRNVPRLVPGWTKPIIIGRHAYGDQYRATDFRVPGPGRLSLVFVGAD
                     GRKIEHEVFEFPGPGVTLAMYNLDASIRDFARASMNYALARQYPLYLSTKNTILKAYD
                     GRFK"
BASE COUNT          796 a         1283 c         1277 g          809 t
ORIGIN      
        1 ccgcgatcgg ccgcaccgca tcgcgcactt cgatgaggcc cttgagcagc atctgccagg
       61 cgcgcgcgag ggcggagagc ggcagcttcg cagccatctc cgtggcccgc cgcgcctcgc
      121 ctgatccgcc gtcgaagaaa ccctcggcgg cgggtgcgac tttcaaccgg gtcagatagt
      181 gggtgatctc cagcaggtcc tgcaccacga caagcggatc ggcgccgccg gcatagatgt
      241 cgccaaaatc ggccagcgcc gcggcgatct ctccgcgcat cgtcttttcg aacaggtcga
      301 ggatgcggcc gcggtcggcg aggcccagca tggcgcgcac gcttccggcg ccaatcgcat
      361 gaccttcctc atgcgcgatc gcctggtcga gaagcgacag cgcatcgcgc accgagccct
      421 ctgccgcccg cgcgatcagc gacagcgccg ccggctcgat ttcgacattc tccttcgccg
      481 cgatgtttga gagatgcgag acgagttcgc ccgtctcgat ccggcgcagg tcgaagcgct
      541 ggcagcggga caggatggtg acgggaacct tgcggatctc ggtggtggcg aagatgaatt
      601 cgacatgggc gggcggttcc tcgagcgtct tgaggagcgc gttgaacgcc tgcttcgaca
      661 acatgtgcac ttcgtcgatg atgtagactt tgtagcgggc gcttgcgggc gcgtagcgga
      721 cgctgtcgat gatctggcgg acttcctcga tgccgttgtt cgaggcgccg tccatttcct
      781 gcacgtcgag atggcgcgat tccgcgatcg ccacgcaggg ctcgcagacc ccgcagggtt
      841 gaatcgtcgg ttccttgcgc gtgccgtcgg ggccgacgca attgagcgcg cgcgcgatga
      901 tgcgcgccgt tgtcgtcttg ccgacaccgc gcacgccggt gagcatgaag gcgtgggcga
      961 tccggcccgt gctgaaggcg ttgcgcagcg tgcgcaccag cgcttgctgg ccgatcaggc
     1021 cggtgaaatc ctgcggccgg tatttgcgcg ccagcacgcg gtaagcctcg gccttcgtgg
     1081 gcgccggggc aaagccgagt gtgggttctt gcgtgtcgtc catggtttcg tgtcgggatt
     1141 ggccaacgcc actatagcaa agccgctatc gcaggcgagc cgaattcctc ggacggcggc
     1201 tctccggcgc atgcagggat tgcttgaaaa caaagcagct gaaaatcgtc aggtgagagc
     1261 ccggccgcga cccgatgcgg attcgttacg gctgcttcct tccggacctg accggattgg
     1321 cgagcgcgtc gtccgccgcc ggactcccga agtgaatata gtgccctagg tgctgagaag
     1381 cgagtcggac ttgacgaaga gtcatagacg aagctgctta agtctcttct tggacgaaaa
     1441 cccaaaaggc acctcgaacg ccgcaccgcc cgtgaccaat ttggcctgtt cgatgatttt
     1501 tggccaccag cgcaccatgg cggcggccta gttccagtga gactgcgaat tccaaactcg
     1561 tgacaacatg aatacgatca atccggatct tctcagggcc tcttgctcga tcggcctgcg
     1621 cgccatggca cgatcttgcg tgacgactgc ccagccggat tcggatgaaa gcttgtgtat
     1681 ccaagttatg tcgggagtat tgggcgcaaa tatctctctt aaatgtttaa cagaatgacc
     1741 gtcaggagag gaaagcgcat cgagtgcttt agcgagatgc ggcgagatat ttgcgtcaca
     1801 gaaaaatttc acgcagccag ccgcttttcc cattcaacag cagcatcgac ggcttctttc
     1861 ggcacgccgt accaacacgc tgcgatttcg gctgagtttt ctgcgcatgc cgcggcagca
     1921 aggctatcgg ccggcacgtc gcccgacagg gggcgcccaa atgcgcgctg gggatcaatc
     1981 acgattgtct tccggggcca tgccggatac cagagttttg gttcgtcatc gtctccgaag
     2041 acgatctgct tgaaaagcga cggtgcaatg acgctatgga aagcaagttg attgcgggcg
     2101 agatcaatca actttggctc gtcggattgc ttcgcaattt cgaggaaaat cgtgcgccca
     2161 tccgtacgga atcgtcttga aagaaatggg tgccaaggcc tatcgatcgt ctgggtgttc
     2221 cgggcagcct gccggatcac gtgccaggag acacctgcct ctcggaacgc attcacgacg
     2281 cgagcttcaa gcagatcgcg gaatcccaca atacactggc cctcgattct tccaatttca
     2341 ggtgctgtca acggcgcgta ttcaacgcgc gcgcaattgc gccgccgcgc gtagccgaag
     2401 acccatcgcc ggaacgtctc cgccggaact ccagtgatcc tggaagcttc tggaacggta
     2461 tagagacctt tcccaataag gttcgtgtcc gctcgggatt gaattgtgct cattaaagct
     2521 cctttgctga gccagaatca caaaactgcg tgttgataaa gagcgctttg cggacgcaat
     2581 gttatcatca aatcagcgag actcttcagt ggcttgccga ccaagtcgta gttcggcgga
     2641 gattgtcaac actggcgagc tgctcagaaa gtggcgttca gcttctccga acccggtgct
     2701 ttcctttggc gagcgccacg atcatgccgc gcagggtctg cacctcctga taggtcaggc
     2761 gggcacgatg cagcgtggcg cggatggcgc gggccatccc gtcgaccttg tcgggcgggt
     2821 agaggaagcc gctggcgagc agctcgcgct ccagatgttc gaacagctgc agcatctcct
     2881 cgcgcgtcgg cttgcgctgg agtggaccgt gctccagccg cgccggcgga gttgcatcac
     2941 ccgcgcggaa ccattcatag ctcgtcacga gcaccgcctg accgagattg agcgacgcaa
     3001 attcggccgt ggggacggta atcaccgcgt cgcagagcga gatttcgtca ttgtcgagcc
     3061 cggcgcgctc attgccgaac aggatgccgg ttcgctcgcc gcgcatggac gcaacccgca
     3121 gccttcttgc cgcctccgcc ggggtcagaa cctcccttgc gattccacgt tcccgcgctg
     3181 tcgttgcgaa tacgaggttc agctctccga tcgcttcggc gaccgtcgcg aacaccagcg
     3241 caccgtccac aacatttgcg gcgccggccg ccatcgctgc ggccttcgcg ttgggccatc
     3301 cggcacgcgg cgcgacgaga cgcagatccg acaatccgaa attcgccatt gcccgcgccg
     3361 cggcgccgat gttctcgccg agctggggat gagagagaat gacggccggc gggatcattt
     3421 cggttcgagt cgaaacaccg ttctttgacg caatccggcg acctcgctat agcgggcgcc
     3481 gcttcgcgcg aggatatcca tgaacaagat caaagtcgcc aatcccgtcg tcgagctcga
     3541 tggcgacgag atgacgagga tcatctggga gctgatcaag agaaagctca tcctgcctta
     3601 tctcgacatc cagctcgaat atttcgatct cggcatcggg caccgcgacg cgaccggcga
     3661 ccaggtgacg atcgacgccg cggaagccat caagcgcacg ggcgtgggcg tcaagtgcgc
     3721 gacgatcacg cccgacgagg cgcgcgtcgc cgagttcaag ctcgggaaga tgtggaaatc
     3781 gcccaacggc acgatccgca acattctggg cggggtgatt ttccgcgagc cgatcatctg
     3841 ccgcaacgtg ccgcggctgg tgccgggctg gaccaagccc atcatcatcg gccgccacgc
     3901 ctatggcgat cagtaccgcg cgacggattt tcgcgtaccg ggacctggtc gattgagcct
     3961 tgtcttcgtt ggcgcggatg gacggaaaat cgagcatgaa gtgttcgagt ttcccggccc
     4021 gggcgtcacc ctcgccatgt acaatctcga cgcttcgatc cgcgattttg cccgcgcgtc
     4081 gatgaactac gcgctcgcgc gccaataccc gctctacctc tcgaccaaga acacgattct
     4141 caaagcctat gatggccgtt tcaag
//
LOCUS       DASZMC010000174         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_70336,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000174 DASZMC010000000
VERSION     DASZMC010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70336"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VG274_03460"
     CDS             complement(<1..993)
                     /locus_tag="VG274_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWE05737.1"
                     /translation="MNRFAHFLALLAWLMTPRFAVASEYHGVVQFGGLPVPGATIAMS
                     RGDQKFTAVTDPQGAYVFADLADGTWMIEVVMPGFAAVHGDVNVAPGAAASAWELKML
                     PFAEVMKDIPATVAATPAPAAANATATASAAASSNNRNRKTARVATPQRPGGFQRADV
                     NASGSEAASAADAVPSNTGVPANTNDADLASRASDGFLINGSANNGAASSFSQSAAFG
                     NNRRGSGSLYNGSLGLIFDNSAFDARSYSLTGQDTPRPAYEQTKGVFTLGGPLSIPHL
                     LPTGPMFFVGYQWTRNRNANTQSALVPTLAEREGDAGGGVALPTAQINPQAQALL"
BASE COUNT          322 a          505 c          441 g          300 t
ORIGIN      
        1 cagcagagcc tgtgcctgcg ggttgatctg cgccgtggga agcgccacgc cgccgcccgc
       61 gtcgccttcg cgctcagcca gcgtcggtac cagcgccgat tgcgtgttgg cgttgcgatt
      121 gcgcgtccac tgatagccca cgaaaaacat cggcccagtg ggcagcagat gaggaattga
      181 cagcggtccg cccagtgtga acacgccctt cgtttgctcg taggcaggcc ggggcgtatc
      241 ctgccccgtc agcgaatagg agcgcgcatc gaacgcggaa ttatcgaaaa tcaaacccaa
      301 gcttccgtta tacaagctgc ccgagccccg ccggttgttg ccgaaggcag cggactgcga
      361 aaatgacgac gctgcgccat tgttcgcgct gccattgatc aggaatccat ccgaagctcg
      421 cgacgcaaga tcggcatcgt tcgtattcgc aggaaccccg gtgttcgatg gaaccgcgtc
      481 tgccgcggac gccgcctcgc ttccgcttgc gttcacatcg gcgcgctgaa atccgcccgg
      541 cctctgcggg gtcgcaacgc gcgctgtttt tcggttgcga ttattggacg atgccgcggc
      601 gcttgccgtc gccgtcgcat tcgctgctgc gggagcgggc gttgcggcaa ctgtcgccgg
      661 aatatccttc ataacctcgg cgaacggcag catcttcaat tcccatgccg acgccgccgc
      721 gcccggcgcg acattcacat cgccgtgcac cgccgcgaaa cctggcatca cgacctcgat
      781 catccatgtg ccatccgcga gatcggcaaa cacatacgcc ccttgcggat cggtaacggc
      841 cgtaaatttc tgatcgcctc gactcatcgc aatcgtcgca ccaggcacgg gcagccctcc
      901 gaattgcacg acgccgtgat actccgacgc cacggcgaag cgcggggtca tcagccaagc
      961 aagaagagcc aggaaatgag cgaatcgatt catctaaaaa aacccgagaa acccgccttt
     1021 agctttccga cggcctctcg acatggtcta acaccaggat atcggtcggc gcctccgtcg
     1081 attccagccg caagcccaac tgctgttgaa atgccgtgaa caaatccgag gcgccggcat
     1141 tgtacgttgc cggcggcgat tgaatccgcg tgcattgcca ggtgcccgtg agctcgtgag
     1201 ccgacagcgt tcgcgccgac catcatccac agagcgagtc ttttcatggc gccctcaacc
     1261 cacttcccat cgccccacta aaccgtcaca attctaaacg gacataagcc gcaaacgaag
     1321 ccttgtctgg ctggaaagca agtagacgaa atcggactgc cgttcgttcc atgtcacttg
     1381 gtttttcggc gaagtgtcga ggcaggcact gccggtttat cggaaggggc gggatgtagc
     1441 tcgcgagtaa ggcggatcga ggtatcgctc cgtgttgact cacagtccct gagccgaatc
     1501 aattctcaga cggcttctcg atatgatcaa taacgatcac atcgatcggc gccctggtcg
     1561 ataccagc
//
LOCUS       DASZMC010000175         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_70571,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000175 DASZMC010000000
VERSION     DASZMC010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70571"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..860)
                     /locus_tag="VG274_03465"
     CDS             complement(237..860)
                     /locus_tag="VG274_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWE05738.1"
                     /translation="MVDRLARLQLARAAREFATRTNSSLPGLVLLTDDEHMTDPLASI
                     SALPRGGLVICRSRDRARRIALARSISNLAHTRGLRWIIADDPVLAANLGAHGAHFPE
                     RKLTLAARWRVRRPDWMLTCAAHSLSACARAARIGADAVLLAPAFATLSHPNRSCIGP
                     LRLRLIATRLDVPIYALGGVDAHRAKRLAGARLAGLAAIGGLACSER"
     gene            909..1553
                     /locus_tag="VG274_03470"
     CDS             909..1553
                     /locus_tag="VG274_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWE05739.1"
                     /translation="MADILARIDLARKAALAPAPSTTLVAISKTFSPEQIRPVLAFGH
                     RVFGENRVQEAKAKWPGLKAEWPDVALHLVGPLQSNKVREAVSLFDAIHSVDRPKIAE
                     ALHDEIQRSGRRPSLFIEVNTGEETQKAGTSPHEAPRFIERCRDELRLPIVGLMCIPP
                     AGEAPAPYFALLRKIAQANGLDQLSMGMSSDFEIAIRFGATHVRVGTAIFGARE"
     gene            complement(1704..>2204)
                     /gene="ribA"
                     /locus_tag="VG274_03475"
     CDS             complement(1704..>2204)
                     /gene="ribA"
                     /locus_tag="VG274_03475"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HWE05740.1"
                     /translation="EPEHIAIVIGDPPRDAPVLARLHSECFTGDLLGSLRCDCGVQLR
                     EALAAIAKAGGGVLLYLAQEGRGIGLINKLRAYRLQDQGFDTVEANERLGFESDERLY
                     GIAARMLTLLGFKSVRLMTNNPHKVDALRQAGIDVIERVPHTFPPNVHNRFYLNTKAE
                     KAGHLI"
BASE COUNT          412 a          695 c          720 g          377 t
ORIGIN      
        1 cgcccgttgt tgccggcggc caaggccgcc cgaagatccc ccgcgaggcg gcttatacgg
       61 tcccgcccgg aagtctgcaa cggctcgcgg ccggacgagg tgggaaacgc ccgtgatctg
      121 atgcggccga atccggggtc catctcttca ggggccggtt acgcgagaga ccatagacgg
      181 cggcatgcac cgcctcggcg tcaccggctc tatgttcgcg gatcacgaaa acggctctag
      241 cgttcagaac acgcaaggcc gccgattgcc gcaaggccgg cgagtcgcgc acctgcgagc
      301 cgttttgcac ggtgggcatc aacgccgccg agcgcataga tcggcacgtc cagccgcgtt
      361 gcaatgagac gcagccgcag cggtccgatg cagctgcggt ttggatggct cagcgtggcg
      421 aacgcgggcg ctaacaagac cgcgtcagcg ccgatgcggg cggcgcgtgc gcacgcggag
      481 agagaatgcg cggcgcaggt gagcatccag tccgggcgcc ggacgcgcca acgggctgca
      541 agagtgagct tccgctcggg aaaatgcgcg ccgtgtgcac ccagattcgc ggcaagcacc
      601 gggtcgtcgg caatgatcca gcgcaggccg cgcgtgtggg cgaggttcga gatcgagcgc
      661 gccagtgcga tgcggcgagc gcggtcgcgc gaacgacata taacgaggcc tccgcgcggc
      721 aatgccgaga tcgaagccag cggatccgtc atgtgctcgt cgtcggtcaa aagaacgagg
      781 ccgggcagag aagaattcgt ccgcgtcgcg aactcgcgtg ccgcccttgc caattgcaac
      841 cgtgcgagcc tatccaccac gcgatgtcct cgccgcctgc ttcggccgat tcaccagccg
      901 ctcaaaacgt ggcggacatt cttgcgcgca tcgacttggc gcgaaaggct gcccttgctc
      961 ccgccccttc gacgacgctg gtcgcgattt caaagacctt ttctcccgaa cagatacgtc
     1021 cggtgctggc gttcggccac cgggtcttcg gcgagaatcg cgtccaggag gcgaaagcaa
     1081 agtggcccgg cctgaaagcc gagtggccgg atgtcgcgct gcatctcgtc gggccgttgc
     1141 agtccaacaa ggtccgcgaa gcggtctcgc tgttcgatgc catccattcc gtcgaccggc
     1201 cgaaaatcgc cgaggccctg cacgacgaaa ttcagagaag cgggcgaagg ccatccctct
     1261 tcatagaggt caacaccggc gaagaaacgc agaaggccgg cacttctccg cacgaagcgc
     1321 cacgcttcat cgagcggtgc cgcgacgagc tccggctgcc catcgtcggg ctgatgtgca
     1381 ttccgccggc cggcgaggcg ccggcgccgt attttgcact cctgagaaaa atcgcccagg
     1441 caaacgggct cgatcagctc agcatgggaa tgagttcgga tttcgagatt gccatccgct
     1501 tcggcgccac ccatgtccgc gtcggaacgg caatcttcgg cgctcgtgag taaatcaaat
     1561 cagaatttta acgcagagga cgcggaggat gcgcagtggg cgcagaagac aagggttgcg
     1621 cgccgaaggc gcgcgattct tcttcccccg cgtcctccgc gcatcctccg cgtactctgc
     1681 gttaaatctt tttggtgcgt gcgctatata aggtgtcccg ccttttctgc cttcgtgttc
     1741 aggtagaaac gattgtggac gtttggcggg aaagtatgtg gcacgcgctc gatgacgtcg
     1801 attccggcct gccgaagcgc gtcgaccttg tgcggattgt tggtcatcag gcgcaccgat
     1861 ttgaatccga gcagggtcag catgcgggct gcgatgccgt aaaggcgttc gtcgctttcg
     1921 aaaccgaggc gctcattggc ctccacggtg tcgaagccct gatcctgcag ccggtaggcg
     1981 cggagcttgt tgatgaggcc gatgccgcgg ccttcctgcg cgaggtacag aagaacgccg
     2041 ccgccggctt tggcaattgc ggcaagcgct tcgcgcagct gcacgccgca atcgcaacgg
     2101 agcgatccca acagatcgcc ggtgaagcat tccgaatgca gccgcgcgag aacgggggca
     2161 tcgcgcggcg gatcgccgat cacgatcgcg atgtgctcgg gctc
//
LOCUS       DASZMC010000176         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3377289,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000176 DASZMC010000000
VERSION     DASZMC010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377289"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            527..1294
                     /locus_tag="VG274_03480"
     CDS             527..1294
                     /locus_tag="VG274_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWE05741.1"
                     /translation="MERWRALGLDTSPHADLIAQRLSHFVELSGREIALLESLAQRTD
                     ESAAGSELIREGQLLDSPRLLMAGWACRYRTLPDGRRQIFKFILPGDLFGFCLRPKAV
                     ALAATIALTPVVTADASPLAAAISKDGASHPGLTQACYATVSLDEAYLLNQLVRVGRQ
                     TAYERTAHFILEIRERLSMVGLAGETSVAIPLTQEIMADALGLSVVHLNRTLQQLRRN
                     QLIEFKGGMVRLLQPQRLAELANFRAPHVTEAARAAA"
     gene            complement(1517..>3518)
                     /gene="malQ"
                     /locus_tag="VG274_03485"
     CDS             complement(1517..>3518)
                     /gene="malQ"
                     /locus_tag="VG274_03485"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWE05742.1"
                     /translation="TANDEAVIALAAEAGLDVRWTGADGKSCVVAIDTLASVLRALGL
                     PAGSHAEVRESRTRLRMENRGVPALIAARAGETVRLPKGSAGEINLASGARRSLRLRD
                     TGDGAVCFRAPSEVGYHRIELDGRDVTLAVAPGHCVRPDDIVAHSRLAGLSAQIYALR
                     GGTSGAFGDFAALGAFAKRAGRMGFEALMTSPVHAQFGVACSRFSPYSPSTRFFLNPL
                     FADATLEGGEPLEDTQRESCLIDWPEASSRKHQALRAAFARFRKRGNRSAFEHFCRDG
                     GERLLSHALFEALGRRFRSRGNYERCDWPAGFSAPGAPGVREFAHSAREDVEFQLFLQ
                     WITACSAAAAQKAARQTMAIGIVSDIAVGMDPQGSHAWSAPQELLQGLRVGAPPDAFA
                     SSGQDWGLTTLCPRTLRASAYAPFIATLRAAMHNAGGIRIDHALGLRRMWVLPAGASS
                     VDGVYLRYPQPELLRLAALESHLHRAIVIGEDLGTVPGGFRDDLADAGVLGTQVLWFE
                     RDRAGRFTSPRQWRRNAVATTTTHDLATIAGWWSENDIDWQVRIHRLDGAGGKMRDER
                     AADRGRLWSALKRAKCVTGSKPERNKPDAVVTGALAFVGRTRRTLALAPVEDIAGEVE
                     QANLPGTIDEHPNWRRRMKRGDFFRNKRARASLKAFVAARQT"
BASE COUNT          643 a         1160 c         1113 g          602 t
ORIGIN      
        1 acgatctgct cgagtttcat gtcggcatag cgtgtccccg cgacgagctt gttgagcgtc
       61 tcgacatagg tcttgtgatg cttgcggtag tggaaaccga cagtgcgcgc agaaatcgca
      121 ggtgcaagtg cgtcttcatc atacggcaga ggcgacagct caaacggact ggacatcgga
      181 gaactccatg ctgaaagacg gaaccgacgc gcaccgcggg agggtcgatt cggacctctc
      241 tttgccgttc tccggctctt tccaggcgat gcgcgccaca gattcaaagc ccgagaaaag
      301 cgatggttcc ccgctttccg cgggaacctg gcggcggccg gaaggcggcg aacgtgactg
      361 caaacacgca ggcgcgtgag gcgaaaccgc aacgaaaccc ggctttcgtt cgtttgcagc
      421 cttgagtctt atcgcaggct aagaaggggc ggcacgcgcg ctgggacgaa ccgcgagagg
      481 atcgtgcttg tcggtcaccg gaacacatgg catgcggaga acgaccatgg aacgctggcg
      541 cgccctgggg ctcgatacgt caccacatgc cgatctgatc gcccagcggc tgtcgcattt
      601 cgtggaactt tcgggccgcg agattgcgct tctcgagtcg ctcgctcagc gcaccgacga
      661 atcggcggca ggaagcgaac tgatccggga aggacaattg ctcgattccc cccggctcct
      721 gatggcgggc tgggcctgcc ggtatcgtac cctgcccgac gggcggcgcc agattttcaa
      781 attcattttg ccgggagacc tcttcggctt ctgtcttcgg ccgaaggcgg tggcgctcgc
      841 cgcgacgatc gcgctgacgc cggtagtaac tgccgacgcg tcgcccctgg ctgcggcgat
      901 ctccaaggat ggcgcatcgc atcccggtct gacccaggcc tgctacgcga ccgtcagcct
      961 cgacgaggcc tatcttctca atcagctcgt gcgtgtgggc cggcaaacgg cttatgagcg
     1021 aaccgcgcat tttatcctgg agatccgcga acggctttcc atggtggggc tggccggcga
     1081 aaccagcgtg gcgatcccgc tcacccagga gatcatggcc gacgcactcg ggctgagcgt
     1141 cgtgcatctc aatcgcacgc tgcagcagct gaggcgcaat cagctgatcg aattcaaggg
     1201 cgggatggtg cggctgctgc aaccgcaacg ccttgccgaa cttgccaatt tccgcgcccc
     1261 gcacgtaacc gaggcggcgc gcgcggcggc ataagccgtg caagtagaat tcacggacca
     1321 tgttttctct ccccacatgg gggagggcat ctgcacctca cggcattact ccaagattcg
     1381 tcatcgccgg gcccccgggc cgcgcttcgc gcggagcggg ggcccggcga tgatgtaatt
     1441 tggaggatgt gcagatacct tagccaacgt ggcgggaaga gagcgcgggt cgcattgcgc
     1501 ccaaacacga gcgcgctcac gtttgccttg ccgcaacaaa tgccttcaga ctcgcgcgag
     1561 ctctcttgtt gcgaaagaaa tcgccgcgct tcattcggcg ccgccaattg ggatgctcgt
     1621 cgatcgtgcc gggaaggttt gcctgttcca cctcgccggc aatgtcctcg acgggcgcaa
     1681 gcgcgagcgt gcggcgcgtg cggccaacga acgctagcgc gcctgtgacg accgcatcgg
     1741 gcttgttgcg ttccggtttg ctgcccgtca cgcatttcgc gcgcttgagc gccgaccaaa
     1801 gccgcccgcg atccgcggcc cgttcgtccc gcattttgcc cccggccccg tccaggcgat
     1861 gaatgcgtac ctgccaatcg atgtcattct cgctccacca gccggcaatc gtcgcgagat
     1921 cgtgcgtcgt ggtggtcgcg accgcattgc gccgccattg gcgcgggctg gtaaatcgcc
     1981 cagcacggtc gcgttcgaac cacagcactt gcgtgccgag gacgcctgcg tcggcgagat
     2041 cgtctcggaa tcccccgggc acggttccca aatcctcgcc gatgacaatc gcgcggtgaa
     2101 gatgcgattc gagagccgca aggcgcagca actccggctg cggatagcgc agatagacgc
     2161 catcgacgga tgaggcgcct gcggggagga cccacatcct ccgcaggccc agcgcatggt
     2221 cgatgcgaat gccgcccgca ttgtgcatgg cggcgcgcaa ggtggcgatg aaaggcgcat
     2281 aggccgacgc ccgcaatgta cgcggacaca acgtcgtcaa tccccagtcc tggccgctgg
     2341 acgcaaacgc gtcgggtggc gcgcccacac gcaggccctg cagcaattcc tgcggtgcgc
     2401 tccaggcgtg gcttccctgc gggtccattc cgacggcgat gtcggaaacg atgccgatcg
     2461 ccatggtctg ccgcgccgct ttttgggcgg cggcagcact gcacgcagtg atccattgca
     2521 ggaagagctg gaattccacg tcctcgcggg cagaatgcgc gaattctcgc acgccaggtg
     2581 cgccgggagc gctgaaacct gcaggccaat cgcaccgctc gtagttcccc cgggaacgaa
     2641 agcgccggcc gagcgcctcg aacagcgcat gcgacagcag gcgctcaccg ccgtcccggc
     2701 aaaaatgctc gaatgcggaa cggttgccgc gcttgcgaaa ccgcgcgaat gcagcgcgaa
     2761 gcgcctgatg tttcctcgaa ctcgcctcgg gccagtcgat cagacatgat tcccgttgcg
     2821 tgtcttcgag cggctcgccg ccctcgagcg tcgcatcggc gaacaatggg ttgagaaaga
     2881 accgcgttga cggcgaatag gggctaaagc gcgagcacgc aactccaaac tgcgcatgga
     2941 cgggactcgt catgagggct tcgaagccca tgcggccggc gcgtttcgca aaggcgccaa
     3001 gcgctgcaaa atcgccgaac gccccgctcg ttccaccacg cagcgcgtag atctgtgcgg
     3061 acaggccggc gagccggcta tgcgcaacga tgtcgtcggg acgcacgcaa tgcccgggcg
     3121 ccaccgccag agtgacatcc cggccgtcga gctcgatccg gtgatagccc acttcgctgg
     3181 gcgcgcggaa acagacagcg ccatcgcccg tgtcgcgcag tcgcaaactc ctacgcgcac
     3241 cgctcgcgag attgatttcg ccggcgctac ccttcggcaa acgcacagtc tcgcccgccc
     3301 gcgcggcaat cagcgccgga accccccggt tctccatgcg caggcgggta cggctttccc
     3361 tgacttcggc gtgcgaaccg gccggcaggc caagcgcccg taagacggac gcgagagtat
     3421 cgatggcaac gacgcagctc tttccatctg cgccggtcca tcgcacgtcc agtccggctt
     3481 ccgccgcgag cgcgatgacg gcttcgtcgt ttgccgtc
//
LOCUS       DASZMC010000177         4550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3092173,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000177 DASZMC010000000
VERSION     DASZMC010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3092173"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VG274_03490"
     CDS             complement(<1..560)
                     /locus_tag="VG274_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05743.1"
                     /translation="MSHSHAVARPIGTMTLPRRLRGLAGHVRLTVIVCLVLICGSFAS
                     AAMIQMRLDRTHALAQAAAFGERRAQEMATDLATALDRYRATGAAFASAEASAETSAA
                     LSEAGGAALRNIAVLDANGRIVSEMRGAPQGLLPLSMGALEQARGGRAIVASRDAGSI
                     AILFAQENRIVAVQIDSRRLLSPAGM"
     gene            929..1237
                     /locus_tag="VG274_03495"
     CDS             929..1237
                     /locus_tag="VG274_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05744.1"
                     /translation="MSEVSPGGLHMKYGIDFRYLPKGAADPLDNSYPVDVEVDESQFA
                     LIPALGDHVCIPGDNPGMRNVPLKGRVRSRLFHYRLGYCYVTIVVEDTDETWAGLGGK
                     "
     gene            complement(1241..3874)
                     /gene="pepN"
                     /locus_tag="VG274_03500"
     CDS             complement(1241..3874)
                     /gene="pepN"
                     /locus_tag="VG274_03500"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWE05745.1"
                     /translation="MRTEEPRAVRLKDYKPYDYRIREIALDFALAPEATRVVARMQVE
                     RITPEPAPLVLSGEHLKLLSLELDGRALAADEFALGAETLTIHAPPARFGLEIATEIA
                     PARNTALEGLYVAKGIFCTQCEAEGFRRITYFPDRPDVLSVYTTRIEAAKDELPVLLS
                     NGNPVEHGDLPGGRHFAVWHDPFPKPCYLFALVAGDLGHIADEFTTMSGRRVALHIYV
                     EHGNEPRALYAMDSLKRAMRWDEEAYGREYDLDIFMIVAVSAFNFGAMENKGLNIFND
                     KVLLASPETATDDDYARIEGVVAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQSFS
                     AAMRSPAVKRIDDVRVLRMRQFQEDAGPLAHPVQPQAYITIDNFYTATVYEKGAEVIG
                     MLKTLVDEEGYRKATDLYFARHDGHAATVEDWVRCFEDACGRNLGQFRLWYAQAGTPV
                     VDAEGVYDSEKKTYALKLTQNLAPTPGQTDKKPMHMPVRLGLAGQSGRALPLTLDGEN
                     ATGPEERVLELTGREQSFLFVDVPEPPLLSIGRRFSAPATFRTNTDRPGRATLMARDR
                     DAFNRWESGQALAREVLIEMAGAAPSGRVPIPDPILVDAMGEVLERAPEDRAFAALML
                     TPPLESEIALAMQPADPEAIHAARTDLIRAIAAAHRGAFERIHDARDAAEQFSPDAES
                     AGRRALGNACLRYLTAADDEAAARRGDAHYRGASNMTDMMAGLAQLSRMNSPLREIAF
                     AHFYDRFRDNALVLDKWLGLHASSPLPGTVHGVRALMKHPFFDIANPNRVRALVGAFS
                     GNHLRFHAADGQGYALVGEALRTLDAINPQVAARMAGAFENWRRYDAPRQALMRTELE
                     AMLAMPKRSSNLFEVATKMLG"
     gene            complement(3939..4472)
                     /locus_tag="VG274_03505"
     CDS             complement(3939..4472)
                     /locus_tag="VG274_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05746.1"
                     /translation="MAKANFHKNQRVYVKPVGTWALIERVVPHWAKGIDEPLRVYYDV
                     GLGREFAADELQAEQPLAENGHRGDENWRVTRARNKWQPSEDCARHPVPGTYPIVITG
                     QAEWGGWRVPGAEYDLDPLRIERQARLIASVPLLENFAQELVEWARKSGEEMPAALAD
                     LAHQAQDILSNVDKPQS"
BASE COUNT          784 a         1455 c         1497 g          814 t
ORIGIN      
        1 tgcatgccgg cgggcgagag cagccggcgc gaatcgatct gcactgcaac gatgcggttt
       61 tcctgcgcga agaggatcgc gatgctgcct gcgtcgcgtg aagcaacgat tgcgcggccg
      121 ccgcgcgcct gttcgagcgc gcccatcgaa agcggcagca agccttgcgg cgcgccccgc
      181 atttccgaaa cgatccggcc gttcgcgtcg agcaccgcta tgttcctgag cgcagcgccg
      241 cctgcttccg acaatgctgc ggacgtttcc gcgctcgctt cggcggaggc gaacgcggca
      301 ccggttgccc ggtagcggtc gagcgcggtc gcgagatcgg ttgccatttc ctgcgcgcgg
      361 cgctcgccga acgccgctgc ctgggcgagc gcatgcgtgc ggtcgagccg catctggatc
      421 atcgccgccg aggcaaagct gccgcagatg agaaccaggc agacgatcac ggtcaggcgt
      481 acatggccgg ccagtccacg cagcctgcgg ggaagagtca tggtcccgat cggcctggcg
      541 acggcatggc tgtgcgacat gaaccgggct cggcgcctca gtgattcgcg aatcaccgat
      601 tatgcggatt gccgactcgc tgtcgaatgc ggcggccctc gcccgcgact gagccgaatt
      661 ccgcgcacgt gttccccctc acccgcacct gagcagaact cattgcccgt atctgcattc
      721 ctctcccaca aggggagagg ggaaattgga aacctgcagc agcgttggaa aatcagcgcg
      781 tgcggccccc tctccccttg tgggagaggg ctcaagcaga gggcctccac aagcgtacag
      841 gcgcaggtga gggggaacac gtacgccgaa gtcggcgcga gcgggggagc cgtcttccaa
      901 aagcaccgcg aaactgcaac catggaacat gagcgaggta tcgcccggag gcttgcacat
      961 gaaatacggc atcgatttcc gctacctgcc caagggcgcg gccgacccgt tggacaattc
     1021 ctatcccgtt gatgtcgagg tcgacgagtc gcaattcgcg ctcatcccgg cgctcggcga
     1081 ccatgtctgc atccccggcg acaatccggg catgcgcaac gtgccgctca aaggccgggt
     1141 gcgcagccgt ctcttccact atcgcctcgg ttattgctac gtgacgatcg tcgtcgaaga
     1201 cactgacgaa acctgggccg ggctcggcgg aaaataatat tcagcccagc atcttggtcg
     1261 ccacctcgaa caggttcgag gagcgcttgg gcatggcgag catggcttcg agttccgtac
     1321 gcatcagcgc ctggcgcgga gcgtcgtagc ggcgccagtt ttcgaaggcg cccgccatgc
     1381 gcgcggcgac ctgcggattg atcgcgtcga gggtgcgcaa tgcctcgccg accagcgcat
     1441 agccttgccc gtccgcggcg tgaaagcgca gatgattgcc ggagaacgcg cccaccagcg
     1501 cgcgcacgcg attgggattc gcgatgtcga agaagggatg cttcatcagc gcacgcacgc
     1561 cgtgaaccgt tcccggaagc ggcgatgacg cgtgcaggcc gagccatttg tccagcacca
     1621 gcgcgttatc ccggaagcgg tcgtaaaaat gcgcgaaggc gatttccctg agcggggaat
     1681 tcatgcggga gagctgggca aggcccgcca tcatgtcggt catgttggac gcgccgcggt
     1741 aatgcgcatc gccgcgccgc gccgccgctt cgtcgtccgc ggcggtcaga tagcgcaggc
     1801 aggcattgcc gagcgcccga cggccggccg attcggcgtc gggactgaac tgttccgccg
     1861 catcgcgcgc gtcatggatt cgttcgaatg cgccgcggtg tgccgccgca atggcgcgga
     1921 tcaggtcggt gcgcgccgcg tggatcgctt cgggatccgc gggctgcatc gccagcgcga
     1981 tttcgctttc cagaggaggc gtgagcatca gcgcggcgaa ggcgcgatcc tctggcgcgc
     2041 gctcgagcac ttcgcccatg gcgtcgacga ggatcgggtc gggtatcggg acacggcctg
     2101 aaggcgcggc gccggccatt tcgatcagca cctcgcgggc aagcgcctgg cccgattccc
     2161 agcgattgaa cgcgtcacgg tcgcgcgcca tcagcgtggc gcgtccgggc ctgtcggtat
     2221 tcgtcctgaa cgtcgcgggc gccgagaacc tccgaccgat cgagagaagc ggaggctccg
     2281 gcacatcgac aaacaggaag ctctgttccc gtccggtgag ttcgaggacg cgttcctcgg
     2341 gaccggtcgc gttctcgcca tcgagcgtca ggggcaacgc gcgcccgctc tgtcccgcaa
     2401 ggccaagacg caccggcatg tgcatcggtt tcttgtcggt ctgacccggc gtcggagcga
     2461 gattctgcgt cagcttgagt gcgtaggttt tcttttcgct gtcgtacacg ccctcggcgt
     2521 ccacgaccgg cgtgcccgcc tgcgcatacc agagacgaaa ctggccgaga ttgcggccgc
     2581 acgcatcctc gaaacatctg acccaatctt ccacggttgc cgcgtggccg tcgtggcggg
     2641 cgaaatacag atcggtcgcc ttgcggtagc cttcctcgtc caccagcgtc ttcagcatgc
     2701 cgatcacttc ggcgcctttt tcgtagaccg tcgcggtgta gaaattgtcg atggtgatgt
     2761 aggcctgcgg ctgcacggga tgggccagcg gccccgcatc ctcctggaac tggcgcatgc
     2821 gcagcacgcg cacgtcatcg atgcgcttta ccgcgggcga gcgcatcgcg gccgaaaagc
     2881 tctggtcgcg gaagacggtc aggccctcct tcagcgacag ctggaaccag tcgcggcagg
     2941 tgacgcggtt gccggtccag ttgtggaaat attcgtgggc gaccacgcct tcgatgcgcg
     3001 cgtaatcgtc gtccgtcgcg gtttccggac tcgcgagcag gaccttgtcg ttgaaaatgt
     3061 tgaggccctt gttttccatg gcgccgaaat tgaacgccga cacggcaacg atcatgaaaa
     3121 tgtcgagatc gtattcgcgg ccgtaggctt cctcgtccca gcgcatcgcg cgcttcaggc
     3181 tgtccatcgc atatagcgcg cggggctcgt tgccgtgctc gacatagatg tgcagcgcga
     3241 cgcgccggcc cgacatggtc gtgaactcgt ccgcaatatg gccgagatcg cccgccacca
     3301 gcgcgaagag atagcagggc ttggggaagg gatcgtgcca gaccgcgaaa tgccgtcccc
     3361 cgggaagatc gccatgctcg acgggattgc cgttcgagag cagcaccggc agctcgtcct
     3421 tcgccgcctc gatgcgcgtc gtgtacacgc tgaggacgtc cggccggtcg ggaaaatagg
     3481 tgatgcggcg gaaaccttcg gcctcgcatt gcgtgcagaa gatgcccttg gcgacgtaaa
     3541 gcccttcaag cgccgtgttg cgcgcgggcg cgatctcggt cgcgatttcg agcccaaagc
     3601 gcgcgggtgg agcgtggatg gtgagcgttt cggcgccgag cgcaaattcg tcggctgcga
     3661 gtgcgcggcc gtccagctcg agcgacagga gtttcagatg ctcgccgctc aggacaagcg
     3721 gcgcaggctc gggcgttatg cgttcgacct gcatacgcgc caccacgcgc gtcgcctccg
     3781 gagcgagcgc gaagtcgagc gcgatttcgc ggattcgata gtcgtacggc ttgtaatcct
     3841 tgaggcggac ggcgcgcggt tcttcggtgc gcatgtgggc cctcgatgcg atgggccgcc
     3901 gtcttagggc atatttggca ggaacggaac cgccggaatc agctctgggg cttgtctacg
     3961 ttcgaaagga tgtcctgcgc ctgatgtgcg agatctgcga gtgcggccgg catctcctcg
     4021 ccggacttgc gcgcccattc gaccagttcc tgcgcgaagt tttcaagcag gggcacgctg
     4081 gcgatgaggc gggcctggcg ctcgatgcgg agaggatcga gatcgtactc cgcacccggg
     4141 acgcgccagc cgccccactc ggcctgcccc gtgataacga tcggataggt gcccgggacg
     4201 ggatggcgcg cgcaatcttc cgatggctgc catttgttcc gagcgcgcgt aacgcgccaa
     4261 ttctcgtcgc cgcgatggcc gttctcggca agcggctgtt cagcctgcag ttcgtccgcg
     4321 gcgaattcgc ggcccagccc cacatcgtaa tagacacgca ggggctcatc gattcccttc
     4381 gcccaatgcg gcaccacgcg ctcgatgagc gcccatgtgc ccaccggctt cacgtaaacg
     4441 cgctggttct tgtggaaatt cgccttggcc atccgagcca aaatcccttt tcgacccccc
     4501 taagataaag aggtgccctt aaatggtggt taaagcgatg cgcgcggttc
//
LOCUS       DASZMC010000178         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3634784,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000178 DASZMC010000000
VERSION     DASZMC010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3634784"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1252
                     /locus_tag="VG274_03510"
     CDS             53..1252
                     /locus_tag="VG274_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HWE05747.1"
                     /translation="MSYIVASVAGVGFFVMSVLLLAVWPGMVLEKQTQAMSPAHPLDL
                     TPSERHGREIYGREGCAYCHTQQVRYLHSDMTRFGAPTLAWETRFDYPQLWGTRRIGP
                     DLARESGSRSMDWQFAHLYSPRALVVDSVMPPFSAMFDSSPDRPKQDARDLVAYLETL
                     GRDRELAGPEGEAHAREACKCDNDEMTQMAFHAPELNANPRKTRRKGPVPSLLPGDLG
                     RGRALYARNCASCHGVKGEGDGPGSLGGLHPPPANLAEHFYRYNRLAFALVNGIDGTS
                     MPAWRDLSFIDLSALAETVRSFYIEKPEAPVSKEVLDLGERVYKENCVRCHGPEGAGD
                     GTAVSQLQIVPANLRDGRASLELTLRVLRNGVDGTQMAPWTPRLSDAEIEAVARYTRT
                     LLPRQLE"
     gene            1274..1468
                     /locus_tag="VG274_03515"
     CDS             1274..1468
                     /locus_tag="VG274_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05748.1"
                     /translation="MIDLIVFSSIGFLAVFFIAWMLRPDLRTWIEKPKHRFQANVQSY
                     DQEQKAAASGERPRPEATSR"
     gene            1465..>2084
                     /locus_tag="VG274_03520"
     CDS             1465..>2084
                     /locus_tag="VG274_03520"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWE05749.1"
                     /translation="MTGSEKSGLWYAIITCCAFGAVVIATVLVISQQNLLSPNSVKPP
                     SIATEAYGRHLVMQTAELLGPDVKDPNLRYNGSRLNCASCHLDVGREPGTLTLLLATE
                     HYPRFSGRVGAKTDIEDRINECMTRSMNGRAVPRDSPEMMAMAAYLRSLGGINQEMGE
                     SLRKPQEPPAFKTPNRAASPEAGQKVFAEHCAECHGMNGQGLLATA"
BASE COUNT          398 a          587 c          712 g          387 t
ORIGIN      
        1 gccgagtaag cccccggagg ggcgcagccc cgattcaggg aaggttctgc gcatgtccta
       61 tatcgtggcg agcgttgctg gcgtgggctt cttcgtcatg tccgtgctgc tgctggccgt
      121 gtggccgggc atggtgctgg aaaagcagac gcaggcgatg tcaccggcgc atcctttgga
      181 tcttacgccc agcgagcggc acgggcgcga gatctacgga cgcgaaggct gtgcgtattg
      241 ccacacgcag caggtgcgct acctgcattc cgacatgacg cgtttcggcg cgccgacgct
      301 ggcctgggaa acacgcttcg attatccgca gttgtggggc acgcgccgca tcggtccgga
      361 tctggcgcgc gagagcggct cgcgttccat ggattggcaa ttcgcgcact tgtattcgcc
      421 gcgggccctg gtcgtggatt cggtgatgcc gccgttttcg gcgatgttcg atagttcgcc
      481 ggatcgaccg aagcaggatg ctcgcgatct ggtggcgtat ttggagacac ttgggcgcga
      541 tcgggaactg gcgggtcctg agggcgaggc gcacgcgagg gaagcttgta agtgcgataa
      601 cgacgagatg acgcagatgg catttcatgc gccggagctg aatgcgaatc ctcgcaagac
      661 gcgccgcaag ggtccagtgc cgtcgctgtt gccgggcgat ctggggcgcg gaagggcgct
      721 ctacgcgcgc aactgcgcga gctgccatgg cgtgaagggc gagggcgacg gccctgggag
      781 tttaggagga ttacatccgc cgccggcgaa tctggccgaa catttttatc gctacaaccg
      841 gctggcgttc gcgctggtga acggcatcga cggcacgtcc atgcctgcgt ggcgagatct
      901 cagctttatc gatctttccg cgctggcgga aactgtgcgc tctttctaca tcgagaagcc
      961 ggaggcgccg gtgtcgaaag aggttctgga tttgggcgag cgcgtataca aagagaattg
     1021 cgtgcgatgc cacggaccgg aaggtgcggg cgacgggaca gcggtcagcc agcttcagat
     1081 tgtgccagcc aatttgcgag acggacgcgc cagcctggaa ctgacgctgc gcgtgctgcg
     1141 caacggcgtc gacgggacac agatggctcc ctggacgcct cggctgagcg acgcggagat
     1201 tgaagcggtg gcgcgctaca cgcgcacgct tctccccagg caactggaat aagaggatgg
     1261 cctcgggcat tagatgatcg acctcatcgt attctcatcg atcggattcc tggcggtatt
     1321 ttttatcgcg tggatgctgc gtcccgacct gcggacgtgg atcgaaaaac ccaagcaccg
     1381 ttttcaggcg aatgtgcaaa gctatgatca ggagcagaaa gcggcggcga gcggcgaaag
     1441 gccgcggcca gaagcgacaa gcagatgaca ggcagcgaga aaagcggact gtggtacgcc
     1501 atcattacgt gctgtgcgtt tggtgccgtc gtcatcgcca ccgtcctagt catctcgcag
     1561 cagaatcttc tctcgcctaa ctccgtgaag ccgccgtcga tcgcgacgga agcttatgga
     1621 cgccacttgg tcatgcaaac ggcggagctg ctcgggccgg acgtgaaaga tccgaacctg
     1681 cgctataacg gaagccggtt gaattgcgcg tcgtgccacc tggatgtggg ccgggagccc
     1741 ggcacactga cgctgctgct ggccaccgag cattatccgc gcttctccgg gcgcgtcggc
     1801 gctaagaccg atattgaaga tcgcatcaac gagtgtatga cgcgcagcat gaacgggcgc
     1861 gcggtgccgc gcgacagccc tgagatgatg gcgatggcgg cgtatcttcg ctcgctcggc
     1921 ggaatcaacc aggaaatggg tgaatcgctg cgcaagccgc aggagccccc tgcgttcaag
     1981 acgccgaatc gtgcggcgag tcccgaggct ggacagaaag tatttgccga gcattgcgcg
     2041 gaatgccatg gcatgaacgg ccaggggctg ctggcgactg caga
//
LOCUS       DASZMC010000179         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4740142,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000179 DASZMC010000000
VERSION     DASZMC010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4740142"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1269
                     /gene="dprA"
                     /locus_tag="VG274_03525"
     CDS             136..1269
                     /gene="dprA"
                     /locus_tag="VG274_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWE05750.1"
                     /translation="MRQELETSLKAHPLSDSERRAWLRLARAQNVGPVTFAALIERFH
                     SASAALRELPRLARRGGAAGDLRIPGEDEAERELETLHRAHGRLIASIEPDFPTGLAA
                     LEAPPPLISVLGRHEILAREMVAVVGARNASALGRKFATELASDLGRAGLGVVSGLAR
                     GIDTAAHEGALATGTVAVLAGGVDDIYPPENARLYARIVDTGAVVSEMPIGQKPQAKH
                     FPRRNRIISGLARGVVVVEAAEGSGSLITANFALEQGREVFAVPGSPLDPRAKGTNRL
                     LRDGAALTEGAGDVLDVLQPLIGHAFREPTDGSIAWAAVADDRGETKLREKIEELLGP
                     APVVVDELVRQCAAPAAAVLSILIEFELAGRLHRESGGRVSLG"
     gene            1482..>3917
                     /gene="topA"
                     /locus_tag="VG274_03530"
     CDS             1482..>3917
                     /gene="topA"
                     /locus_tag="VG274_03530"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWE05751.1"
                     /translation="MNILVVESPAKAKTINKYLGPGYRVLASFGHVRDLPAKDGSVKP
                     DQDFAMSWEVDARAQKRLNEIADAVKNADKLILATDPDREGEAISWHVLEFLKQKRAL
                     GHAHVERVVFNAVTRSAVLEAIAHPRNINEELVDAYLARRALDYLVGFTLSPILWRKL
                     PGARSAGRVQSVALRLVCERESEIEAFRPQEYWTIDADLRASSGSFAARLTLLDGKKL
                     EKLSLRTEADATRARDAIAAQHFAVGEIETKPLKRHPAPPFITSTLQQEASRKLGFAA
                     KRTMQIAQHLYEGVEVGGETVGLITYMRTDGVQMAGEAIGEARHVIGENYGARYVPEA
                     PRAYSSRAKNAQEAHEAIRPTSFSRRPGQLLRSLEPDHARLYELIWKRALASQMQSAE
                     LERTTIDAVSGDRKITLRATGTVTLFDGFLALYQEGKDDENDEDGARLPKLAKNDRLD
                     IEKISPAQHFTEPPPRYSEASLVRKLEELGIGRPSTYATILSVLRDRAYVRMDRGRFV
                     PEDKGRIVTSFLKSFFSRYVQYDFTADLEEKLDEVSAGALPWKQLLRDFWKDFSAAID
                     EAKDLRVSAVITELDEMLGPHIFPAAETGEDPRKCPSCGAGALSLKLGRFGAFIGCSN
                     YPECRYTRQLGQTAGEAANGQPQQLGEDPGTGEAITLRTGRFGPYVQRGNGEKPARGS
                     IPKGMDPASVDLEYALRLLSLPREVGKHPESGEAITANFGRYGPYVAHQGQYASLDSP
                     EDVFTVGINRAVSLLAEKKAKSKTFRGAEALKELGNAPGGAAVKLMRGRYGPYVTDGA
                     TNATVPRDVEPLSV"
BASE COUNT          749 a         1250 c         1250 g          668 t
ORIGIN      
        1 ggaaggacgc agaggaatat gaattgcgcg cctctggcgt gcgacctttt ccacggcgta
       61 ccctgcgcat cccctgcgca tcctctgcgc cctctgcgct aaaaactttc tttgtgcgct
      121 tcgtagtaat ttggcgtgag acaagaactg gagacatccc tgaaagcaca tccgctttcc
      181 gattccgaac ggcgcgcgtg gctgaggctt gcgcgcgcgc aaaatgtggg accggtcaca
      241 ttcgcggcgc tgatcgagcg atttcattcc gcgtctgcgg cgctcaggga attgccgcga
      301 ctggcaaggc gcggcggcgc ggcgggtgat ctcagaattc caggcgaaga tgaagcggag
      361 cgcgaactcg aaacgcttca tcgcgcccac ggccggctca tcgcctccat cgagccggat
      421 tttcccaccg ggcttgcagc actcgaagcg ccgccgccgt tgatttcagt gctcggacgc
      481 cacgagattc tggcgcgcga gatggtcgcg gtggtcggcg cgcgcaacgc atcggcgctc
      541 ggccgcaagt tcgcaacgga gctcgcgtcg gatctcggcc gggcaggtct tggcgtcgtt
      601 tcaggactcg cccgcggcat cgatacggcg gcgcacgagg gcgcgcttgc aacgggcaca
      661 gtcgcggtgc tcgcgggagg agtcgacgac atctatccgc ccgaaaacgc gcggctgtat
      721 gcgcgaattg tcgataccgg cgccgtggtg tccgaaatgc ccatcggcca gaagccgcag
      781 gcaaaacatt tcccgcgccg caaccggatc atttccggat tggcccgcgg tgtcgtggtg
      841 gtggaggcgg ccgagggttc gggctcgctg atcaccgcca attttgcgct cgagcagggc
      901 cgggaagtct tcgcggtgcc gggctcgccg ctcgatccgc gcgccaaggg cacaaaccgg
      961 cttctccgcg acggcgccgc gctcaccgaa ggagcgggcg acgtgctcga cgtattgcag
     1021 ccgctgatcg gacacgcatt tcgtgagccg accgatggtt cgatcgcgtg ggcggcggtc
     1081 gccgatgacc ggggcgagac caaattgcgc gaaaaaatcg aagagctcct cggtccggcg
     1141 cccgttgtgg tcgacgagct ggttcggcaa tgcgccgccc cggccgctgc cgtgctctcc
     1201 atcctgatcg aattcgagct cgccggccgg cttcaccggg aatccggagg ccgcgtttcg
     1261 ctcggatagc gggggcggag aaacgaccca gacttcccgc aaaaattgaa attcgcagca
     1321 gataaagtca aaaatacaac tacaagttat actccacgaa attccggcga gttgacaggc
     1381 cggagtccgg cttcctatgt ccgcgccgtc acgaatcgcg cttagtggtg ttacccagcg
     1441 atggcacctg tgcgcgccgg ccaaggcagt cctctatcag catgaacatt ctcgtcgtcg
     1501 aatcccccgc caaggccaag acgatcaaca agtatctggg ccctggctac agggttctgg
     1561 cgagcttcgg ccatgtccgc gaccttccgg cgaaggacgg ctcggtgaaa ccggaccagg
     1621 acttcgcaat gagctgggag gtggatgcgc gggcccagaa gcgcctgaac gagatcgcgg
     1681 acgccgtcaa gaacgcggac aagctcattc tcgcaaccga ccccgaccgc gagggcgagg
     1741 cgatatcctg gcacgtgctg gagttcctga agcagaagcg cgcgctcggc catgcccatg
     1801 tcgaacgcgt cgtcttcaac gccgtgacgc ggtccgccgt gctggaggcc attgcccatc
     1861 cacgtaacat caacgaggag ctggtcgacg cctatctcgc ccgtcgcgcg ctcgactacc
     1921 tggtcggctt cacgctttcg ccaattctct ggcgaaaact gccaggcgcg cgctcggcgg
     1981 gacgcgtcca gtccgtggcc ctcaggctcg tctgcgaacg cgaaagcgag atcgaagctt
     2041 tcaggccgca ggaatactgg acgatcgatg ccgatttgcg cgcgtcttcg gggtcgtttg
     2101 ccgcgcgcct gacccttctg gacggcaaga agctcgaaaa gctctccctc cgcacggaag
     2161 ccgatgcaac acgcgcgcgc gatgcgatcg ccgcgcagca tttcgccgtc ggcgaaatcg
     2221 agacgaagcc gctcaagcgc caccccgccc cgcccttcat cacctcgacc ctgcaacagg
     2281 aggcttcgag gaagctgggg tttgccgcca agcgcaccat gcagatcgcc cagcacctct
     2341 acgagggcgt cgaagtcggc ggcgaaacgg tcggactcat tacttatatg cgcaccgatg
     2401 gcgtgcagat ggcgggcgag gcgatcggcg aggcgcggca cgtgatcggc gagaattatg
     2461 gcgcgcgcta tgtgcccgag gcgccgcgcg cctattcgag ccgcgccaag aacgcgcagg
     2521 aagcccacga ggcgatccgt ccgacaagct tctcgcgccg gcccgggcag ctgttgcgca
     2581 gcctcgaacc cgatcatgcg cgcctttacg agctgatctg gaaacgcgca ctggccagcc
     2641 agatgcagag cgcggaactc gaacgcacga cgatcgacgc tgtttccggc gatcggaaga
     2701 tcacgctgcg cgctaccggc acggtgacgc tgttcgacgg atttctcgct ctctatcagg
     2761 agggcaagga cgacgaaaac gacgaggacg gcgccagact tcccaagctc gcaaaaaacg
     2821 accggctcga tatcgagaag atctcgccgg cgcagcactt caccgagccg ccgccgcgct
     2881 actcggaggc cagcctggtg cgcaagctcg aagagctggg catcggacgg ccttcgacct
     2941 acgccacgat cctttcggtg ctgcgcgatc gcgcctatgt gcggatggat cgcggacgtt
     3001 tcgtgcccga ggacaaaggc cgcatcgtca cgagcttcct caagagcttc ttcagccgct
     3061 acgtgcaata tgatttcacc gcggatctcg aggagaagct cgacgaagtg tccgccggcg
     3121 cgctcccctg gaagcagctc ctgcgcgatt tctggaagga tttttccgcg gcgatcgacg
     3181 aggccaaaga tcttcgtgta tcggcggtca tcaccgagct cgacgaaatg cttggcccgc
     3241 acatttttcc agctgccgag accggtgaag atccgcgcaa atgtccgagt tgcggcgccg
     3301 gcgcattgag cttgaaactc ggccgctttg gcgccttcat cggctgctcg aactatcccg
     3361 aatgccgcta tacgcgccag ctcggacaga cggcgggcga agctgcgaac ggacagccgc
     3421 agcagctggg cgaagatccc ggaacgggcg aggcgatcac gttgcgcacc gggcgtttcg
     3481 gaccttacgt gcagcgcggc aatggcgaaa agccggcgcg cggaagcatt cccaaaggga
     3541 tggacccggc atccgtcgat cttgaatacg ccctcaggct gctgtcgctt ccgcgcgagg
     3601 tgggaaaaca cccggagtcc ggcgaagcga tcaccgcgaa tttcggccgt tatggtccct
     3661 atgttgctca tcagggccag tacgcgtccc tggattcccc cgaagacgtg ttcacggtcg
     3721 gtatcaaccg cgccgtctcg ctgctggcgg agaagaaggc aaaatcgaaa acattccgtg
     3781 gtgcggaggc gttgaaggag ttgggcaacg cgccgggcgg cgccgcggtc aagctcatgc
     3841 gtggccgata cggaccgtat gtcaccgacg gcgcgacaaa tgcgacggtc ccgcgcgacg
     3901 tggagccttt gagcgta
//
LOCUS       DASZMC010000180         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2551349,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000180 DASZMC010000000
VERSION     DASZMC010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551349"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..897
                     /locus_tag="VG274_03535"
     CDS             277..897
                     /locus_tag="VG274_03535"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HWE05752.1"
                     /translation="MEFTGRYVIPATPDQVWAALNDPAVLQLCIPGCESLARDGDHHF
                     VATATLRIGPVKATFKGAIEQSELDPPRRCVLKGEGLGGVAGFARGAAEVVLDREAGS
                     TVLSYAARATIGGKLAQVGQRLIDGAAKQIADDFFARFSAAVATQFRSAPPVEEALLD
                     IPLSQPLDEPPIIADKPDREGLAPEIWVIGLIAIVVILLVLFGMAL"
     gene            968..1444
                     /locus_tag="VG274_03540"
     CDS             968..1444
                     /locus_tag="VG274_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWE05753.1"
                     /translation="MTVVNMTVNGSPVVGDVEDRTLLVYFLRETLRLTGTHIGCDTSQ
                     CGACVVHLDGRAVKSCTMFACQADGASVTTIEGIAHDGTLHPVQEAFREHHALQCGFC
                     TPGMIMTAIDMIRRRKAQDRKTIRRELEGNFCRCTGYHNIVDAIEAAAEHCASDRA"
     gene            1441..2268
                     /locus_tag="VG274_03545"
     CDS             1441..2268
                     /locus_tag="VG274_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWE05754.1"
                     /translation="MIPYQFGYHRPGSIAQAQALFSRLQDARFLAGGQTLIPVMKQRL
                     ANPAHLIDLSRIGELSFVAAEHGAIAIGGGVRHFQVESSPEIRRAIPALAGLAGTIGD
                     PAVRHLGTLGGSLANNDPAADYPAAVLGLGATIATTNNVISADKFFSGMFQTALEEGE
                     IIKTVSFPIPLRAGYAKFPNPASRYAIVGVFVAQTHAGVRVAVTGAAPCVFRAAEMER
                     ALSARFEPHSLSDVKVSSNGLNSDLHASADYRAHLVTVMAKRAVAAALDGPSSPTRE"
     gene            2272..2589
                     /locus_tag="VG274_03550"
     CDS             2272..2589
                     /locus_tag="VG274_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWE05755.1"
                     /translation="MDEADILEAAERWILDGHTVALATVVKTWGSAPRPAGSHIAVRA
                     DGLFEGSVSGGCVEGEIIEAARETIRDGEARYLRFGVSDEKAWSAGLACGGRIEIFVE
                     PIV"
     gene            2614..3348
                     /locus_tag="VG274_03555"
     CDS             2614..3348
                     /locus_tag="VG274_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWE05756.1"
                     /translation="MKRSTLHAVCDAARAGRAIVRAVDIVSGEERLIDIPSGDSQLER
                     EAADAARIDRSAMADADGRSWFLDVVNPPLHLAIVGAVHIAQILARMARLTGYGVRVI
                     DPRGAFASPDRFPDVDLSREWPDEALAKEPLGPRSALVALTHDPKLDDPALAAALRTN
                     CFYIGALGSKKTHAARLARLGAHGFGDPELARIHGPVGLAIGARSPAEIAISILAEMT
                     MKLRADDVWERSIVRATANTGLTPRS"
     gene            complement(3345..4217)
                     /locus_tag="VG274_03560"
     CDS             complement(3345..4217)
                     /locus_tag="VG274_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05757.1"
                     /translation="MKSAVRNTLLLCAALSVGTGVAVARASKAPAPAAQAKQGPDSQS
                     YASRGRFADRFLAEFDTNKDGKVTHDEFNRTLAHEFAVATNGAPAMTLDQFASTHLKD
                     LRQTAAIQFHQIDWNGDGKITLDEYMVPERDRFEFMDRDGSGVVSCGSSRGGGQFGGG
                     APAGRGSGSSSSRGFGSRGRSSLCFSADLDKDGKVTRAEFEKYTQQQFNAFAKGGSLN
                     MEQFYQLQAAQSRAISARVFQRLDRDRDGKLTLAEFAASQEKLFARLDKNNDGVITED
                     ELTSSRRSRVASRN"
     gene            4387..>4895
                     /locus_tag="VG274_03565"
     CDS             4387..>4895
                     /locus_tag="VG274_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE05758.1"
                     /translation="MDRGATMLKDEAKPHVLVVEDARDIREPLARYLREHGYRTTTAA
                     DAQVARKVMKGAAIDLVVLDIMMPGEDGLSLCRSIRESSQIPVVLLTARGEEVDRIIG
                     LEIGADDYIPKPFSPRELVARIGAVLRRTQALPPRQKPPVAEHIRFGDWTLDTGQREL
                     IGKDGMATAL"
BASE COUNT          909 a         1540 c         1519 g          927 t
ORIGIN      
        1 gtcttatgaa gccataaccc ttggcgacat cgaaccactt tatcgagccg gaaacttcga
       61 acgcctcggc tgggacgcca ggcggcgcca cgttcgattc cattccctac cccccgaacg
      121 ctattacctt gtaaatttga ccaaaccgta acactaactt cacgaagaga aaagcaggac
      181 tggcgaagtc ctgtcaaata ttgtccggcc ggcgcaagcg accggtaacc cactgttcca
      241 gaaattcaat atccgtgctg cgatggagct cgagccatgg agtttacggg ccgctacgtc
      301 attccggcca caccggatca ggtgtgggcg gccttgaacg acccggcggt cctgcaattg
      361 tgcattcccg gttgcgaaag cctcgcaaga gatggcgatc accactttgt cgccaccgcg
      421 acgcttcgca tcggaccggt caaggcgacg ttcaagggtg cgatcgagca gagcgagctc
      481 gatccgccgc ggcgctgcgt gctcaagggc gagggcctgg gtggagtcgc cggattcgcg
      541 cggggcgcgg cagaagtggt tctcgaccgg gaggccggct caacggttct ttcctacgct
      601 gcccgggcaa cgatcggcgg aaagctcgcc caggtcggtc aacggctgat cgacggcgcg
      661 gcaaaacaga tcgccgacga tttctttgcc cgcttttccg ccgcggtggc gacccagttc
      721 cggtcagccc ctccagtcga ggaggcgttg ctcgatatac ccctgagcca gccattggac
      781 gagccgccta tcatcgccga caagccggac cgcgaaggac tcgcgcccga gatttgggtg
      841 atcgggctca tcgccatcgt cgtcattctg ctcgtcctgt tcggcatggc gctgtaatgc
      901 gccttgaccg gactccggtc ctcacgaaag agtatcggat cgcaccgggc atcgagagga
      961 agcattcatg accgtcgtca acatgacggt gaacgggagc ccggtcgtgg gcgacgtcga
     1021 agaccggacg ctgctcgtct attttctccg cgagaccctg cgactcaccg gcacgcatat
     1081 cggctgcgac accagccagt gcggcgcctg tgtcgtccat ctcgacggcc gcgcggtgaa
     1141 gtcctgcacg atgtttgcct gtcaggcgga cggggcaagc gtcacgacga tcgagggcat
     1201 cgcgcatgac ggcacgctgc atccggtgca ggaagcattt cgcgaacatc acgcgctgca
     1261 atgcggattc tgcacgccgg gaatgatcat gaccgccatc gacatgatcc ggcgcagaaa
     1321 ggcgcaggac cggaagacga tccggcggga gctcgagggc aatttttgcc gttgcaccgg
     1381 ctaccacaat atcgtcgatg cgatagaggc cgccgcggaa cattgcgcga gtgatcgcgc
     1441 atgattccct accagttcgg gtaccatcgt cccggctcaa tcgctcaagc gcaggcgctg
     1501 ttttcgcgtt tgcaggacgc aagatttctt gccggcggcc aaacgctgat tcccgtcatg
     1561 aaacagcgcc ttgccaatcc tgcgcacctg atcgatctgt cgcgcatagg ggagctttcg
     1621 tttgtcgcgg ccgagcacgg cgccatcgcg attggcggcg gcgtgcgcca tttccaggtc
     1681 gaaagctcgc ctgaaatccg gcgcgccata ccggctttgg cagggcttgc gggcacaatc
     1741 ggcgatcctg ccgtccgtca tctgggaacg ttgggcggct cgctcgcaaa caacgatccc
     1801 gccgcggact atccggcggc ggtacttggc ctcggcgcga cgattgccac gacaaacaat
     1861 gttatttctg ccgacaaatt cttctccggc atgtttcaga cagctcttga agagggcgag
     1921 atcataaaga cagtttcctt tcccattccg ttgcgagccg gttacgcaaa gtttccaaac
     1981 cccgcgtcgc gctatgcaat agtcggcgtg ttcgtggctc aaacgcacgc cggggtgcgc
     2041 gtcgccgtca ccggtgcggc gccctgcgtc tttcgcgccg cagaaatgga acgggcgctg
     2101 tcagcccgat tcgagccgca ctcgctctcg gacgtgaaag tttcttcgaa cgggctcaat
     2161 tccgatctcc atgcttcggc ggactatcgt gcgcatctgg tgacggtgat ggcgaagcgc
     2221 gccgtcgctg cggctctgga tggcccgtca tcgccgacga gagaatgaac gatggacgaa
     2281 gccgatattc ttgaggccgc ggaacgctgg attctcgacg gccacactgt cgccctcgcc
     2341 acggtggtga aaacctgggg gtctgcgccc cggcccgcgg gcagccacat tgccgtgcgc
     2401 gccgacgggc tttttgaagg atcggtatcg ggcggttgcg tggagggaga gatcatcgag
     2461 gcggcgcggg aaacgatccg cgatggcgaa gcccgttatc tgcggtttgg cgtcagcgac
     2521 gagaaggcct ggtccgccgg tctggcttgc ggaggacgga tcgagatctt cgtcgagccg
     2581 attgtctgac gcctcgcgat cggggggtac cgcatgaagc gcagcacatt gcacgcggtt
     2641 tgcgacgccg ccagagccgg gcgcgcgatc gtgcgcgcgg tcgatatcgt gtcgggcgaa
     2701 gaacgcctga tcgatattcc atccggagac tcgcagctgg aacgggaggc agccgatgca
     2761 gcgcgaatcg atcgcagcgc catggcggat gccgacgggc gatcgtggtt cctcgatgta
     2821 gtcaatccac cgctccatct cgcgatcgtc ggagcggtgc acattgcgca gattctcgcc
     2881 cgcatggccc gtctcaccgg ctatggcgtg cgcgtcatcg atccacgcgg cgcctttgca
     2941 agtcccgacc ggtttcccga tgtcgatctg tcccgcgaat ggccggacga ggcactggcg
     3001 aaggaaccac ttgggccacg cagcgctctc gtcgcattga cccacgatcc caagcttgac
     3061 gatccggctc ttgcggcggc gctgcggaca aactgcttct acatcggcgc gcttggatcg
     3121 aaaaaaacgc atgctgcgag gctggcgcga ctgggcgccc acggcttcgg cgacccggaa
     3181 ttggccagaa tacatggtcc cgtcgggctc gccatcggag cgcgttcgcc ggcggaaatt
     3241 gcgatctcca ttctcgcgga aatgacgatg aagctgcgcg cggacgacgt ttgggagcga
     3301 tccatcgtcc gcgcgacagc caacacgggg cttacccccc gttcctagtt gcgactcgcc
     3361 acgcgactcc ggcgcgagga cgtgagttcg tcttcggtga tcacaccgtc attgttcttg
     3421 tccagccgcg cgaaaagctt ttcctgggaa gccgcaaatt cggcgagcgt gagcttgccg
     3481 tcgcgatccc ggtcgagccg ctgaaagaca cgcgccgaaa tggctcgcga ttgcgccgcc
     3541 tgaagttgat agaactgttc catgttcagg ctgccgcctt tggcgaatgc gttgaactgc
     3601 tgttgggtgt atttttcgaa ctcggcgcgc gtgaccttgc cgtccttgtc caggtcggcc
     3661 gagaaacaca ggcttgatcg gccgcgcgaa ccaaaccccc ggctgctcga actgccgctg
     3721 ccgcggccgg ccggcgcgcc tccgccaaac tgccctcccc cgcgcgacga tccgcaggaa
     3781 acgactccgc tgccatcgcg atccatgaac tcgaagcggt cgcgctcggg caccatgtac
     3841 tcgtcgagcg tgatcttgcc atcgccgttc cagtcgatct ggtgaaactg gattgctgcg
     3901 gtctgacgca gatccttcag atgcgtactt gcgaactgat ccagcgtcat ggccggcgca
     3961 ccgttggtcg ctacggcaaa ctcgtgcgca agcgtccggt tgaattcgtc atgggtgact
     4021 ttgccgtcct tgttggtgtc gaactcggca aggaaacgat cggcgaaacg gccgcgcgat
     4081 gcgtaggact gggaatcggg gccttgcttg gcctgtgcgg caggcgccgg agccttgctc
     4141 gctcgagcga cggccacgcc ggtacccacc gaaagcgcgg cgcacaacag cagggtattt
     4201 cgaactgcag atttcattga agtcatctcc tcatggcgct ccccaatggg cgatgtgata
     4261 tccgcccctc cgtcgcagaa ctttgtccaa tgacccccga actcgtcgca atgtgccccg
     4321 tccggctagg gagcggcgac agactgagac aattcccgat tgcgccggac ggccggaggc
     4381 tgcatcttgg atcgaggtgc aacgatgctc aaagacgaag caaaaccgca tgtgcttgtc
     4441 gtggaggatg cgcgcgatat tcgcgagccg ctggcgcgct atttgcgcga acacggttat
     4501 cgaacgacga cggccgcgga cgctcaggtt gcccgaaagg tcatgaaggg cgccgcgatc
     4561 gacctggtcg tgctcgatat catgatgccc ggcgaggacg gtctcagcct gtgccgttcg
     4621 attcgcgaat cctcgcaaat ccccgtcgta ttgctgaccg cgcgcggaga ggaagtcgat
     4681 cgcatcatcg gtctcgaaat cggcgccgac gactacattc caaagccgtt cagcccacgc
     4741 gagctcgtcg cccggatcgg cgcggtgctc cgccgcaccc aggccttgcc gccccggcaa
     4801 aaacctcccg ttgccgaaca catccgtttc ggcgactgga cgctcgatac cggacaacgc
     4861 gaactcatcg gcaaggatgg aatggctacg gcgct
//
LOCUS       DASZMC010000181         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_358997,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000181 DASZMC010000000
VERSION     DASZMC010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358997"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VG274_03570"
     CDS             complement(<1..935)
                     /locus_tag="VG274_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HWE05759.1"
                     /translation="MIVLAALGSHAVAAETAPLLSEKPPRSAATTEFQARTSTIQPSL
                     TIAYDVLADAANAAADRFSGPQSGKARIGCNQLAFGGATPSAPSVKVTLFKGCVDFDW
                     HVKAVRNGNITVKRDGSGVAVAVPVKFDGNGGFSGDLAKAIQIDNKKFSGTFVVSISG
                     MVRLDKSFCPKLDQAVTHFAWGTPPDIDVIGRSCLDAGNGLKACVGPWKFPAGAMMTD
                     QINRSLQSQVDEINGKIPCDKIRDQLKQVWKTWSLPLTLGNSPTFFVSVEPKSLSVPG
                     VQVGDDGIGLVARLEAATAVSATKPADTGPGELPE"
     gene            1171..2223
                     /locus_tag="VG274_03575"
     CDS             1171..2223
                     /locus_tag="VG274_03575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HWE05760.1"
                     /translation="MTRPEELLHPTPNGLYCPPGDFIIDPVRPAPRAVITHGHGDHAR
                     PGHRAVLATRETLAIMAARYGDGFAEAAQPVRYGETSSHNGVEVTLVPAGHVLGSAQV
                     VLRWKGLTAVVSGDYKRRRDPTCAAFEPVPCDVFVSEATFGLPVFRHPDDAGEIVKLL
                     KSVAQFPERAHLVGAYALGKAQRLICLLREAGYRKTIFVHGALATLNALYEAHGVSLG
                     PLAPATTEKREAFAGEIVLAPPDALADRWSRRFADPLPVFASGWMRVRARARQRQVEL
                     PLVISDHADWDELTQTFREVHPEQVWLTHGEDQALLRWASLNGITARTLSLVGHGEED
                     GEPARAKPELRSGEGR"
     gene            2224..>3345
                     /locus_tag="VG274_03580"
     CDS             2224..>3345
                     /locus_tag="VG274_03580"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HWE05761.1"
                     /translation="MRAFSRLLDRLAFTPARNAKLTLLRDYLRQSPDPERGWALAALT
                     GALGFDVAKPTAIRKAIESRMDAQLFALSYDYVGDLAETVALVWPKKTGTNPEAERAE
                     PQLSEVVDTLQSALRHDVQRLLEAWLDALDADGRWALLKLLTGGLRVGVSARLAKQAL
                     ATLGNVDVAEIEEIWHGQHPPYEDLFAWLEGRSGKPTRAAPGRFRPVMLAQALDETAD
                     LAKYSARDFAAEWKWDGIRVQAVSEAGVKRLFSRTGDEIGHSFPDVVAALRFEGIIDG
                     ELLVVRDSAIAPFSDLQQRLKRKMPDANAIAKFPAGIRAYDLLLAGESDLRSLPFRER
                     RARLESLVAHAKVPGLDLSPLIPFDDWLALAALRASPPAG"
BASE COUNT          556 a         1105 c         1072 g          612 t
ORIGIN      
        1 ttctccggca gctcgcctgg acccgtatcg gctggcttgg tggccgacac ggcggtcgcc
       61 gcttccagcc gcgcgacgag cccgatcccg tcatctccca cctgcacgcc cggaaccgaa
      121 agcgatttcg gctcgacact cacaaagaag gtcggcgagt tgccgagcgt gagcggaagc
      181 gaccaagtct tccaaacctg cttcaactgg tcgcggatct tgtcgcaggg aattttgcca
      241 ttgatctcgt cgacctggct ctgaagggaa cggttgatct ggtccgtcat catggctccg
      301 gcaggaaatt tccacggtcc gacgcatgcc ttgagcccgt tgccggcgtc gaggcagctc
      361 cgcccgatga cgtcgatgtc gggcggcgtg ccccaggcga aatgcgtgac cgcctggtcg
      421 agcttcgggc agaacgactt gtcgagccgc accattcccg aaatcgacac cacgaaggtg
      481 ccgctgaact tcttgttgtc gatctgtatg gccttggcga ggtcgccact gaaaccgcca
      541 ttgccgtcaa acttgacggg cacggcgacg gcgactccgg agccgtcccg cttgacggtg
      601 atgttgccgt tgcggacggc cttcacgtgc cagtcgaaat ccacgcagcc cttaaagaga
      661 gtaaccttta cggacggcgc cgaaggagtc gccccgccga acgcaagttg gttgcagccg
      721 attcgtgcct ttcccgactg cggcccggag aagcggtcgg ctgccgcgtt cgcagcatcg
      781 gccagcacgt cgtaggcgat ggtaaggctg ggttggatgg tgctggtgcg cgcctggaat
      841 tcggtcgtcg cggcgctgcg gggcggcttt tccgacagaa gcggggcggt ttcggctgca
      901 accgcgtgcg aaccgagagc cgccagaaca atcatggctg gcgccgccgc ggcggtcttc
      961 aaaattcgga gtccattccc cgtcctggcc atggtctggt ccctctcggt ttcggaatat
     1021 tcggagcacg cgtgccggct ccgcccttaa cgttcgagag gccgtttgcg tttgcgagtg
     1081 ccagatttgc atcggtctgc gcgatcgctg gacgggctca ccgttcgatc gcttgcctct
     1141 cgcaagctcg aatgaacagg tttaagagcg atgaccagac ccgaagagct gcttcacccg
     1201 acgccgaacg ggctttattg cccgccgggc gatttcatca tcgatcccgt tcgaccggcg
     1261 ccgcgcgcgg tgattacgca cggccacggc gatcacgcgc ggcccggtca tcgtgcggtg
     1321 ctggcgaccc gcgagacgct cgccatcatg gcggcgcgct acggagacgg gttcgcggaa
     1381 gccgcacagc ccgtccgcta cggcgagacc agttcgcaca atggcgtaga ggttaccctc
     1441 gtgcccgccg gccacgtgct cggctcggcg caggtcgtgc ttcgctggaa aggacttacc
     1501 gccgtcgttt cgggcgatta caaacgccgc cgcgacccga cctgtgccgc gttcgagccg
     1561 gtaccctgcg acgtcttcgt gtccgaggcg acatttgggc tgcccgtctt ccgccatccc
     1621 gacgatgcgg gcgagatcgt caagctgctc aaatccgtcg cgcagtttcc cgagcgcgcc
     1681 catctcgtcg gcgcctacgc gctcggcaag gcgcagcgcc tgatctgtct gctgcgcgaa
     1741 gcaggatacc ggaaaacaat atttgttcac ggcgcgcttg cgacactgaa cgcgctctat
     1801 gaagcacatg gcgtcagcct cggtccgctc gcgccggcca ccaccgaaaa gcgcgaagcg
     1861 tttgcgggcg agattgttct tgcgccgccc gacgcacttg ccgatcgctg gtcgcggcgt
     1921 ttcgccgatc cgctgcctgt tttcgcctcg ggctggatgc gcgtgcgggc gcgcgcgcgc
     1981 cagcggcagg tggaattgcc gcttgtcatc tccgatcacg ccgattggga cgaattgacg
     2041 caaacattcc gcgaggttca ccccgaacag gtgtggctca cccatggcga ggatcaggca
     2101 ctgctgcgct gggcttcact caacggcatc acggcgcgga cgctgtcgct cgtgggtcac
     2161 ggcgaagaag acggcgagcc tgcccgcgcg aagccggagc tccgctccgg cgaaggcagg
     2221 tagatgcgcg ccttctcgcg tctgctcgat cggctcgcct tcacccctgc acgcaacgcg
     2281 aaacttacac tgctgcgcga ctatctgcgc cagtcgcccg acccggagcg cggttgggcc
     2341 cttgcggcgc tcaccggcgc gctcggcttc gacgtggcga agcccaccgc catccgcaaa
     2401 gcgatcgaat cgcggatgga tgcgcaatta tttgcgctct cctatgatta cgtcggcgat
     2461 cttgcggaaa cagtagcgct cgtctggccg aagaagaccg ggacgaaccc tgaagcggag
     2521 cgggcggaac cgcaactctc cgaagtcgtc gacacgctgc aaagcgcttt gcgtcacgac
     2581 gtgcagcgcc tgcttgaggc ttggctggac gcactcgacg cggacgggcg atgggcattg
     2641 ctcaagctct tgaccggcgg gttgcgggtc ggcgtgtccg cgcggctcgc aaagcaggcg
     2701 ctcgccacgc tcggcaatgt ggacgtcgca gaaatcgagg aaatctggca cggccagcac
     2761 ccgccctacg aagacctttt cgcctggctt gaggggcgca gcggaaaacc cacccgcgcg
     2821 gcgcctggcc gcttccggcc ggtaatgctc gcgcaggcgc tggacgaaac cgccgatctc
     2881 gcgaaataca gcgcgcgcga ttttgccgcg gagtggaaat gggacggcat ccgcgtccag
     2941 gcggtgagcg aagcaggggt caagcgtctc ttcagccgca ccggcgacga gatcggtcat
     3001 tccttccccg atgtggtcgc ggcactccgc ttcgaaggca tcatcgacgg tgaattgctg
     3061 gtggttcgcg acagcgccat cgcgcccttc tccgacctgc agcagcgcct caagcgcaag
     3121 atgccggacg cgaatgcgat cgcaaagttt ccggccggca tccgcgccta tgatctgctg
     3181 cttgcaggcg aatcggattt gcgcagcttg cctttccgcg agcggcgggc gcggctcgaa
     3241 tcgcttgtcg cccacgcgaa agtgcccgga ctcgatctct cgcctctcat cccctttgat
     3301 gactggctgg cgctggcggc cttgcgcgca agcccgcccg cgggc
//
LOCUS       DASZMC010000182         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3919157,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000182 DASZMC010000000
VERSION     DASZMC010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3919157"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VG274_03585"
     CDS             <1..531
                     /locus_tag="VG274_03585"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWE05762.1"
                     /translation="MSSPFIVRISVLFLYIQVIYCNVYSMICGGSPLDLPAPPESSEV
                     STATPSAALDGDTALPYLLTMHTVVELPTFLRQAVKQGMTEGERETIVNFIAENPDAG
                     DPIQRSGGCRKLRFGGKGKGKRGGFRVITFYSGASVPVFLLTVYAKGSLSNLADAQVN
                     ILKALTKTLLEEYARR"
     gene            613..1011
                     /locus_tag="VG274_03590"
     CDS             613..1011
                     /locus_tag="VG274_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05763.1"
                     /translation="MGEAIGFARGELDPSTYRVHVPSDVDVKAVRQKTGLSQDVFAVT
                     YGLSAGTVRDWEQRRRAPDQAARMYLHAISKDPKGVYTIIAGDKRHTVTNRVSVAGKK
                     KHPVSGSVKRKKAPAHAAAKASASRATRSH"
     gene            1303..3084
                     /locus_tag="VG274_03595"
     CDS             1303..3084
                     /locus_tag="VG274_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWE05764.1"
                     /translation="MTYTAPIADMRFAATRLAGFAGIAGLPGGEELSEGLLDSIFEQG
                     GRFASGVVAPLNRVGDRQGSRLENGVVRTPDGWSEAYRAFVAAGWNAVPFDHDHGGQH
                     LPWLVAMALQEMWHSASMAFALCPMLTQAAVEAIDHHGSPALKRRFLPKMISGEWTGT
                     MNLTEPQAGSDLAAVRTRAVRDGDHYRLTGQKIFITYGDHELAENIVHLVLARLPDAP
                     EGVKGISLFVVPKYLVNEDGTPGQRNDLRCVSLEEKMGIHASPTAVLSYGDNGGAFGL
                     LVGEENQGLSCMFTTMNNARLSVGLEGIGIAERALQKAAQFARERVQGREPVNGEMKH
                     VAIIRHPDVRRMLMTMRALTEAARALAYFAAGKLDVSKRHPDPAVRRDAQAIVDLLVP
                     VVKGWGTEIGSEAASLGVQVHGGMGYIEETGAAQFMRDAKIAEIYEGTNGIQANDLIG
                     RKLTRDGGATAKSFIAAMCALDRDLAAAGAELAPLRKALSEGLSALETATDWILARDN
                     PRDVAAGAMSYLRLWGTVAGGWLMAKAALAAREDLAAGEGNPEFLRAKILTARFYGEQ
                     ILPRAASLKSAATAGAATLMEMADTAF"
BASE COUNT          637 a         1036 c         1045 g          551 t
ORIGIN      
        1 atgagttcac cctttatcgt caggatatcc gtgctttttc tctatataca ggtgatctac
       61 tgtaatgtct acagtatgat ttgtgggggg agcccactgg acctcccagc cccaccggaa
      121 tcgagcgaag taagtacggc aacgccgtcg gcggcacttg acggagatac ggcattgccg
      181 taccttctga ccatgcacac ggtagtcgaa ctgccgacct ttcttcgaca agccgtaaag
      241 caaggcatga cggaaggtga gcgtgagacg attgtaaact tcatcgcgga aaaccccgac
      301 gccggcgacc ccattcagag gtcgggcgga tgccgcaagc tccgcttcgg cggcaagggc
      361 aaaggcaagc gcggcgggtt tcgcgtgatc acgttctatt ccggcgcgag cgtgccggtg
      421 tttttgctga ccgtgtatgc gaagggaagt ctcagcaatc tggccgatgc gcaagtgaac
      481 attctaaagg cactcacaaa gacgcttttg gaggaatacg cgagacgata atcagggaga
      541 atataaccga tggcccggaa gcgacctgta acaaagagcc atgacgtaat cttcggagag
      601 atggtgaccg gattgggcga ggcgatcggc tttgcccgcg gtgaactcga tccttctacc
      661 taccgcgtgc atgtccccag cgatgtggac gtgaaagccg tccggcaaaa gaccggcctc
      721 tcgcaagatg tcttcgccgt gacctacggc ctgtcggcgg gcaccgtgcg cgattgggag
      781 caacgccggc gcgcaccgga ccaggccgcg cgcatgtatc tccatgcaat cagtaaggac
      841 ccaaaaggcg tttatacgat catcgccggg gacaagcgcc acactgtcac taacagggtg
      901 tctgttgccg gaaagaaaaa gcatcctgtt tccggaagcg tgaagcgcaa gaaggccccg
      961 gctcacgccg ccgcaaaagc ttctgcttcg cgcgccacgc ggtcccactg agcggcgctg
     1021 ttacgaccgt caagtaattt ctgcgcgcta acctccccct cgtggggagg tcgaaagacg
     1081 ctcttcgcgt tttccgggcg ggggtaacgg cgcaccgcac agacccccac cccaaatttg
     1141 ctcgcgatgc tcgcaaattt cgacctcccc acaaggggga ggttaaatca aagtgctgcc
     1201 cgctacgcca aaaggcagtc agtctcgaac cgcgtttcgc attcgatttg gtttcttgta
     1261 gaagtctcgg atcgcaaaaa gcccattcgc cgaagagaca aaatgaccta caccgcaccc
     1321 atcgccgaca tgcgctttgc cgccaccagg ctcgcgggct tcgccgggat cgccggcctg
     1381 ccgggcgggg aggagctgag cgaggggctg ctcgattcga ttttcgagca gggcggccgg
     1441 tttgcctcag gcgttgtcgc gccgctgaac cgcgtcggcg accggcaggg ttcgcggctg
     1501 gaaaacggcg tggtgcgcac gcccgatggc tggagcgagg cctatcgcgc cttcgtcgcc
     1561 gcgggctgga atgccgtgcc gttcgaccac gatcatggcg gccagcacct gccctggctg
     1621 gtggcgatgg cgctccagga aatgtggcac tcggccagca tggcgttcgc gctctgcccg
     1681 atgctgacgc aggccgcggt ggaggcgatc gatcatcacg gctcgcccgc gctcaagcgc
     1741 cgcttcctgc cgaagatgat ttcgggcgaa tggaccggca cgatgaacct gaccgaaccg
     1801 caggcgggtt ccgatctcgc cgccgtgcgc acgcgcgcgg ttcgcgacgg cgaccattac
     1861 cgcctcaccg gccagaagat cttcatcacc tatggcgacc acgaactcgc cgaaaacatc
     1921 gtccacctcg ttctggcgcg cctgcccgac gcgccggagg gcgtgaaagg catctcgctg
     1981 ttcgtggtgc cgaaatatct cgtgaatgag gacggcacgc ctgggcagcg caacgatctg
     2041 cgctgcgtgt cgctcgagga gaagatgggc atccatgcga gcccgacggc tgtcctgtcc
     2101 tatggcgaca atggcggcgc cttcgggctg ctggtcggcg aggagaacca gggactctcc
     2161 tgcatgttca cgacgatgaa caacgcccgt ctgtcggtcg gcctcgaggg catcggcata
     2221 gccgagcgcg ccttgcagaa ggccgcccaa ttcgcccgcg agcgcgtgca ggggcgcgag
     2281 cccgtgaacg gcgaaatgaa acacgtcgcg atcatcaggc atcccgatgt gcggcgcatg
     2341 ctcatgacca tgcgggcgct caccgaagcc gcccgcgcgc tcgcctattt cgcggccggc
     2401 aagctcgatg tgagcaagcg tcatcccgat cccgccgtcc gaagggacgc ccaagccatc
     2461 gtcgacctgc tggttcctgt ggtcaaaggc tggggcacgg aaatcggttc ggaagcggcg
     2521 tcgctgggcg tccaggtgca tggcggcatg ggctatatcg aggaaaccgg cgccgcgcag
     2581 ttcatgcgcg acgccaagat cgcggaaatc tacgaaggca cgaacggcat ccaagccaac
     2641 gatctgatcg gccgcaaact gacccgcgac ggcggcgcga cggcgaaatc cttcatcgcc
     2701 gcgatgtgcg cgctcgaccg cgacctggcc gcggcgggcg cggaactcgc accgcttcgc
     2761 aaggcgctga gcgaggggct cagcgcgctc gaaaccgcga cggactggat tctcgcgcgg
     2821 gacaacccgc gcgacgtggc cgccggcgcg atgtcctatc tgcgcctgtg gggaacggtc
     2881 gcgggcggct ggttgatggc gaaagccgcc ttggctgcca gagaggatct ggccgcgggc
     2941 gagggcaacc ccgaattcct gcgggccaaa atcctcaccg cgcgcttcta tggcgagcag
     3001 attctgccgc gtgcggcgag cctcaaatcc gccgccaccg ccggcgccgc cacgctcatg
     3061 gaaatggccg acaccgcgtt ttgatttccg aagtttgcga cggcagagga gaccgggcag
     3121 caccacgtgc cccctccgcc ccttcggagc accccgcaac aagtgcgggg caggctctcc
     3181 cccacgcgct gacgcgcgcg ggggaggaaa tacggctttt cctcccccgc acgtagcaaa
     3241 gcggagtgcg ggggaggtgc cccgaaggg
//
LOCUS       DASZMC010000183         9438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2552318,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000183 DASZMC010000000
VERSION     DASZMC010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2552318"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..543)
                     /locus_tag="VG274_03600"
     CDS             complement(1..543)
                     /locus_tag="VG274_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HWE05765.1"
                     /translation="MKQFLYLLPVLGFGALVYLFFHSLGGPPQDELPSALIDKPAPAT
                     TLPPLDSKATAFTSADLRAGQVTVLNVWASWCVPCRAEAPALAALSRVKGVALFGMVY
                     KDSATAARGYLKDVGNPFSRIDIDRDGRAGIEWGVYGVPETFVVDRAGVVRLRYAGPI
                     VGDALDRVILPAIERARAAS"
     gene            complement(540..695)
                     /gene="ccmD"
                     /locus_tag="VG274_03605"
     CDS             complement(540..695)
                     /gene="ccmD"
                     /locus_tag="VG274_03605"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HWE05766.1"
                     /translation="MRFLAMGGYAGFVWPAYAISLAGVAAAVALTLRSYARAKAMFAT
                     IESDKAR"
     gene            complement(695..1414)
                     /gene="ccmC"
                     /locus_tag="VG274_03610"
     CDS             complement(695..1414)
                     /gene="ccmC"
                     /locus_tag="VG274_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HWE05767.1"
                     /translation="MFELANPKRFMDVSRPLIPWLGAIAAISIGIGLYLALFVVPPER
                     QQGDTVRIMFLHVPAAWIAMMAYALMAVCSLIGLVNRHPVADVAAREAAPLGALFTAL
                     ALVTGSLWGKPMWGAWWVWDARLTSFLLLLFLYLGYIALWNAIENETRAAKAAAILAL
                     VGVVNLPVIEFSVQWWTTLHQGESIFRTGGPTIAPVFLWPLFIMAGGYTALFATLWII
                     RIRTAILQRSVRARLAAAIGG"
     gene            complement(1622..2284)
                     /gene="ccmB"
                     /locus_tag="VG274_03615"
     CDS             complement(1622..2284)
                     /gene="ccmB"
                     /locus_tag="VG274_03615"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWE05768.1"
                     /translation="MSFMALVARDMRLATRAGGSAFLALAFFAMTATLVPLGIGAETR
                     LLSAVAAGIVWICAVLAGLLSLDRLFQADFEDGSLDVLALGPLPLESIAAAKIAAHWL
                     VTGLPLTLVSPMIALLFSLPGRGYGALAAALAIGTPAVSAIGAIGASLTISIRRGGLI
                     LPFIVMPLLAPIVIFGAGAVRAAIDGLASGALLFLAAASLAAVVLSPFAAAAGLRLNL
                     GA"
     gene            complement(2281..2898)
                     /gene="ccmA"
                     /locus_tag="VG274_03620"
     CDS             complement(2281..2898)
                     /gene="ccmA"
                     /locus_tag="VG274_03620"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HWE05769.1"
                     /translation="MGAIAELDVRGIALVRGDRLVLRDLDFHLTAGETLAIGGRNGAG
                     KTSVLRALAGFLQPRSGSIVLRMADGAVVSQAEERRLHIGWLGHQDGVKSPLTPREQL
                     RTHSRFYQCKDDIDDALSRTGLAHLRDFPVQYLSAGQRRRLALARLILGARPLWLLDE
                     PFSALDREGKAIVRQLAESHCATGGMLIAATHEPLGLRGAAIELS"
     gene            complement(2903..3625)
                     /gene="phbB"
                     /locus_tag="VG274_03625"
     CDS             complement(2903..3625)
                     /gene="phbB"
                     /locus_tag="VG274_03625"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HWE05770.1"
                     /translation="MARVAIVTGGTRGIGEAISISLKNAGFKVAALYAGREDCARAFS
                     EKTGIPAYKSDVADFAQCSATVAKINAEIGAIDVLVNNAGITRDATMRRMNRVQWDEV
                     LDANLGGCFNMCKAVWDGMIERGFGRVVNIGSINGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGAAKGVTVNAIAPGYIDTDMVAAVPAPVLEKIVARIPVGRLGHAGEIARGVLFL
                     VADEAGFVTGSTLSINGGQHMY"
     gene            complement(3783..4955)
                     /locus_tag="VG274_03630"
     CDS             complement(3783..4955)
                     /locus_tag="VG274_03630"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWE05771.1"
                     /translation="MTEVVIVSGARTPVGSFNGAFASVPAHDLGKAAIQSALSRAKVA
                     PGEVSEVILGQILTAAQGQNPARQAAVNAGIPVETPGWIVNQLCGSGLRAVALGFQAI
                     VNGDSAIVVVGGQESMSQAPHAAWLRGGQKMGALEFTDTMLKDGLWDAFHGYHMGVTA
                     ENVAREWQITREAQDRFATASQNKAEAARKSGRFADEIVPVTVKERKGERVVDKDEFI
                     RDGVTYDAIASLKAAFQKDGTVTAANASGINDGAAALVLMKGDEARRRGLEPLARIVS
                     WAQAGVDPALMGSGPIPASRKALARAGWTARDLDLIEANEAFAAQACAVNKDMGWDAE
                     KVNVNGGAIAIGHPIGASGARVLVTLLYEMGKRGSRKGLATLCIGGGMGIAMCVEH"
     gene            5159..5761
                     /gene="phaR"
                     /locus_tag="VG274_03635"
     CDS             5159..5761
                     /gene="phaR"
                     /locus_tag="VG274_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HWE05772.1"
                     /translation="MTDSKPGTPDPVVIKKYANRRLYNTKTSSYVTLDHLCEMVKQGT
                     DFEVHDARSGEDITRSVLTQIIFEEESKGQNLLPIRFLRQLIRFYGDSLQAFIPGYLD
                     MSMESFTRQQEDMRHRIAETFGGGSQALEAMTRQNLAMFERTMRMFSPFGIQAAREAE
                     SRSSNGSAESKPASSDEVSALKSEIEAMRRQLAEIARDRK"
     gene            complement(5831..6046)
                     /locus_tag="VG274_03640"
     CDS             complement(5831..6046)
                     /locus_tag="VG274_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05773.1"
                     /translation="MTRQYDLLLSDAEPEGFEDAPERTYAANPDRVRAHRQQILGEAR
                     AADNLPWKPKTVALYRTIFPQMIHWLP"
     gene            complement(6233..7942)
                     /locus_tag="VG274_03645"
     CDS             complement(6233..7942)
                     /locus_tag="VG274_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWE05774.1"
                     /translation="MAFQTLPKRSVDGIRALTAIEDLERLRLAARGAGEAAFDWTIAD
                     DRVDWDGAIDLLALHSEPARMSRGAELRAWMSHAGRERIDAILRQASLEDNFFEIEFE
                     VSSAMGTVWLELRGVRIPGPAGQAERVAGVLRVITERKREAQRLTYLATRDELTGHLN
                     RTSLRSEIARAIEISKAGATNCAYLVAAIDRLAVINEAYGFGAADEVIVTVGERLASA
                     LRASDVIGRIAGNKLGIILGPCTEAEMALAAERMRDAVRGEVIATRRGPVSATISMGA
                     VWLPSDASSGQEAMLRAEEALDRARGAGRNGFWIYTKSPQREMARLRLMAVADEVAEA
                     LETKRLVFAYQPIVCARERRPVHHECLLRMVRADGSIATAGHFIPAAEQLGLVRQVDR
                     HALEMAIAELHAYPEISLAVNVSGTTATDPSWLTSFIHYVRANNGVARRIIVELTETA
                     ALNDFEESSRFISNLRELGCKVAIDDFGAGFTSFRNLQMLRVDIVKIDGAFVRGLSTS
                     PENQIFVRTLVDLAKNFRLKTVAEWVGSEEEAALLASFGVDYFQGYHFGEPTLAPPWK
                     SAG"
     gene            complement(8149..8886)
                     /locus_tag="VG274_03650"
     CDS             complement(8149..8886)
                     /locus_tag="VG274_03650"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWE05775.1"
                     /translation="MTRFATIVATALAATALAAGNSSMAGPKRQVVLELFTSQGCSSC
                     PPADALLGRLADRKDVLALSLPVTYWDMLGWKDTLASEANTRRQKAYAQAMGRGGIYT
                     PQMIVDGASDVVGSREAQVASAIAARTADMRAVPVTLSATPATLHIAIGAGAQRNGDE
                     ATIWIFHILSRTTVHIASGENEGRSIGYRNIVRDVKAIGVWKGQALSLDLPRSDMISA
                     PHDAEAVLVQDGGYGRIIGAGYIGLSQ"
     gene            complement(9026..>9438)
                     /locus_tag="VG274_03655"
     CDS             complement(9026..>9438)
                     /locus_tag="VG274_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05776.1"
                     /translation="KKDGTPLVILAGREYGTGSSRDWAAKGTMLLGVKAVIAESFERI
                     HRSNLIGMEVAPFVFADGKTRRDFGLSGREAIDIRGLSGRIAPAMRLIATIAYPDGRK
                     ADLPLILRIDTADEVAYFENGGILPFVLRNLLAA"
BASE COUNT         1772 a         3117 c         2891 g         1658 t
ORIGIN      
        1 ctagctcgcc gcgcgcgcac gctcgatcgc gggaagaatg acgcggtcca gcgcgtcacc
       61 gacgatgggg ccggcatagc gcaggcgcac cacgcccgct ctgtccacga cgaatgtttc
      121 cggcacgccg tagacacccc attcgattcc agcgcgccca tcccggtcga tgtcgatgcg
      181 gctgaaggga ttccccacat ccttgagata tccgcgcgcc gcagtcgcgc tgtccttgta
      241 gaccatcccg aaaagcgcaa cgcccttgac tctcgatagc gcggcgagtg cgggcgcttc
      301 ggccctgcag ggcacgcacc acgacgccca gacgttcagc acggtgactt ggcccgcgcg
      361 gagatccgcg gaagtgaagg cagtcgcctt gctgtcgagc ggcggaagcg tcgtcgctgg
      421 agcgggtttg tcgatcagcg ctgatggcaa ttcgtcctgc ggtggaccgc cgaggctgtg
      481 aaagaacaaa tagacgagcg ccccgaatcc caggacggga agcaggtaaa ggaactgctt
      541 catcgcgctt tatcgctttc gatcgtggcg aacatcgcct tggcccgggc gtaggaacgc
      601 agcgtcagcg cgacagccgc cgccactcct gcgagcgaga tggcgtatgc aggccagaca
      661 aaacccgcgt aaccccccat cgccagaaat cgcatcatcc gccgattgcc gcggcaaggc
      721 gggcgcgcac gctgcgctga aggatggctg ttcgtatacg gatgatccag agcgtcgcga
      781 aaagagccgt gtagcccccc gccatgatga aaagcggcca cagaaagacc ggcgcgatcg
      841 tggggccgcc ggtgcgaaat atgctctcgc cctggtgcaa ggtcgtccac cactgcacgg
      901 agaattcaat caccgggaga ttgaccacac cgaccagcgc gagaatggca gcggctttcg
      961 cagcccgcgt ctcattctcg atcgcgttcc acagcgcgat gtagccgaga taaagaaata
     1021 acagcagcag aaatgatgtc agccgcgcat cccacaccca ccacgctccc cacatgggtt
     1081 tcccccaaag cgagccggtg acgagcgcaa gcgcggtgaa cagcgccccc agcggtgcag
     1141 cttcgcgcgc cgcaacatcg gcgaccggat ggcggttgac gagcccgatg agcgaacaga
     1201 ccgccatcag cgcataagcc atcatcgcga tccatgccgc cggcacatga agaaacatga
     1261 tgcgaaccgt atcgccctgc tgccgttcgg gcggcacaac aaacaatgcg aggtagagcc
     1321 cgattccgat cgaaattgcg gcgatggccc caagccaagg aatcagtggc cggctcacgt
     1381 ccatgaaccg cttgggattg gcgagctcga acatcgccgc ccatctacgg cctcgcacct
     1441 ttcccggcaa ggcggtgacg cgcgaacctc aaggaaaaag acgaagattc agcgcagagt
     1501 acgcggtaga agcgcagtgg acgcagagta aatagcgcgg cgcgcctttc ggcgcgcaac
     1561 tcttcctctg cgtatccggc gtagcctctg cgtcctccgc gttcaactgc tttctggtag
     1621 cctatgcgcc gaggttgagg cgcaggcccg ccgccgcggc gaagggcgag aggacaactg
     1681 cagcgagaga cgccgcagcg agaaatagga gcgcgccgct cgccaatccg tcgatcgcgg
     1741 cgcgcaccgc cccggcaccg aagatgacga tgggcgccag gagcggcatc acgatgaagg
     1801 gcaggatgag gccgccgcgg cggatcgaga ttgtgaggct tgcgccgata gctccgatgg
     1861 cgctgacggc gggggtgccg atcgcaagtg ccgcggccag cgcgccatat cctcggccag
     1921 gcaatgaaaa aagcagcgcg atcatcggcg agacgagcgt caggggcaga cctgtgacga
     1981 gccaatgcgc ggcaattttc gcggccgcga tcgattccag cggcagtggc cccagtgcca
     2041 gcacgtcgag gctgccatct tcgaaatccg cctggaacag acggtcgagc gacagcagcc
     2101 cggcaagcac cgcgcatatc cagacgatgc cggcggctac cgcgctgaga agccttgtct
     2161 ccgcgccgat gccgagcgga acgagcgtgg ccgtcatggc gaaaaacgcc agcgccagaa
     2221 acgcgcttcc gcccgcgcgc gtggccaggc gcatgtcgcg cgcgacgagc gccatgaagc
     2281 tcatgaaagc tcgattgcag cgccgcgcag gccgagcggc tcgtgcgtgg ccgcaatgag
     2341 catcccccct gtcgcgcaat gggattcggc aagctgccgc acgatcgctt tgccttcccg
     2401 gtcaagcgcg gaaaacggtt cgtcgagcag ccagagaggt cttgcgccca ggatcaggcg
     2461 ggccagcgcg aggcgccgcc tctggccggc ggaaagatac tggaccggga aatcgcgcag
     2521 atgggcaaga ccggtgcgcg acagcgcgtc atcgatatcg tccttgcact gataaaagcg
     2581 cgaatgggtt cgtagctgct cgcgaggagt gagcggggat ttgacgccgt cctgatgccc
     2641 gagccacccg atatggaggc gacgctcctc ggcttgcgaa acgactgccc cgtccgccat
     2701 gcgaagcacg atactgccgg atcgaggctg cagaaacccg gccagggccc gcaggacaga
     2761 cgttttcccg gccccgttgc ggcctccgat cgcgagcgtc tcgccggccg tcaggtgaaa
     2821 atccagatcg cgcaggacga ggcgatcgcc ccgcaccagc gcgatccctc gcacgtccag
     2881 ctccgcgatt gcccccacgt cctcaataca tatgctggcc gccattgatc gaaagcgtcg
     2941 agccggtcac gaatcccgcc tcatcggcga caaggaacag cacgccgcgc gcgatctcgc
     3001 cggcgtggcc gagacggccg accggaatgc gcgccacgat cttctcgagc acgggagcgg
     3061 gcacggccgc gaccatgtcc gtatcgatgt agcctggcgc gatcgcgttg acggtcacgc
     3121 cctttgccgc gccttcctgc gccagcgcct tggtgaaacc gtggattccc gatttcgccg
     3181 ctgcgtagtt gacctggcca tactggcccg cctgcccgtt gatcgagccg atattgacca
     3241 cgcggccgaa gccgcgctcg atcatgccgt cccagaccgc cttgcacatg ttgaagcagc
     3301 cgccgagatt ggcatcgagc acttcgtccc actgcacccg gttcatccgg cgcatcgtcg
     3361 cgtcgcgcgt aatccccgca ttgttgacga gcacgtcgat ggcgccgatt tcggcgttga
     3421 ttttcgcgac cgtcgcggag cattgcgcaa aatcggccac gtcgctcttg taggcgggaa
     3481 tgcctgtttt ctcgctgaaa gcccgggcgc aatcctcgcg ccccgcataa agcgccgcga
     3541 ccttgaaacc ggcatttttc agcgagatcg aaattgcctc gccgatgccc ctggtgccgc
     3601 ccgtaacaat tgcaacgcgc gccattattt cctcccttga ctctctgtca tcgcttgcgc
     3661 gtctccatcc ttcgacaagc tcaggatgag gagtttcctc gccctcgccc tgtcgaaggg
     3721 cgacaagctc aggatgaagg cctcatggtg agcttgtcga accatgatac cgcctgcgcc
     3781 aatcagtgtt ccacgcacat ggcgatgccc atgcctccgc cgatgcaaag cgtcgccaaa
     3841 ccctttctcg atccccgctt tcccatctcg taaagcagcg tcaccagcac gcgggcgccc
     3901 gaggctccga tgggatggcc gatcgcaatg gcgccgccat tgacgttcac cttctccgca
     3961 tcccacccca tgtccttgtt gaccgcacag gcttgggccg caaaggcctc gttggcctcg
     4021 atgagatcga gatcgcgcgc cgtccagccc gcgcgcgcga gcgccttcct cgatgccgga
     4081 atggggcccg agcccatgag ggcgggatcg acgccggcct gcgcccacga gacgatccgg
     4141 gccaaaggct ccagtccgcg ccggcgtgct tcatcgcctt tcatgagcac aagcgcggcg
     4201 gcgccatcgt tgattcccga agcgttcgcg gcggtcaccg ttccgtcctt ctggaaggcg
     4261 gccttgagcg atgcaatggc gtcataggtc acgccgtcgc ggatgaattc gtccttgtcg
     4321 acgacccgct cgccctttcg ttccttgacc gtcaccggca cgatttcatc ggcgaagcgc
     4381 ccggatttgc gggccgcctc ggccttgttt tgcgaggcgg tggcgaagcg atcctgcgct
     4441 tcccgcgtga tttgccattc gcgcgccacg ttctcggcgg tgacgcccat gtgatagccg
     4501 tggaaggcat cccaaagccc gtccttcagc atcgtatcgg tgaattcaag cgcgcccatc
     4561 ttctggccgc cacggagcca tgccgcatgc ggcgcctggc tcatcgattc ctggccgcca
     4621 accacgacga ttgcggaatc gccgttcacg atcgcctgga accccagtgc gaccgcgcga
     4681 aggcccgaac cacacagctg attgacaatc cagcccggcg tttcgaccgg aattccggcg
     4741 ttgaccgccg cctgacgtgc cggattctgc ccttgggcag ccgtcaggat ctgtcccaga
     4801 atgacttccg acacctcccc gggcgcgact ttggcccggc tcagcgcgct ctgaatggcg
     4861 gcctttccca gatcgtgagc cggaacgctg gcaaacgcgc cgttgaacga accgaccggc
     4921 gtgcgtgctc cggacacgat gaccacttcc gtcatgaggt tgcatctttc caattgccag
     4981 accgcaaagt gccgcgattg ggcccgaacg acaagcgccg caatttggcc tcgcgcgcgc
     5041 gccggcgcgc gagctggcgg atcgcccggg ccgcgccggg ccactggaca gcgcaaagga
     5101 tgtaatgttg tgctaggcgg aggatcgggg tcgatccctt tcgcaagctg gtgggaccgt
     5161 gacagatagc aagccaggca cgccggaccc ggtcgtcatc aaaaaatacg caaaccggcg
     5221 gctctacaac acgaagacgt cgagctacgt caccctcgat catctctgcg agatggtgaa
     5281 gcaaggcacg gatttcgagg tgcatgacgc ccgctcgggt gaagacatca cgcgctcggt
     5341 gctgacgcag atcatcttcg aagaagagag caaggggcag aacctgctgc cgatccgctt
     5401 tctgcgccag ctcatccgct tctacggcga tagcctgcaa gccttcattc ccggctatct
     5461 cgacatgtcg atggagagct tcaccaggca gcaggaagac atgcgtcacc ggatcgcgga
     5521 aacattcggc ggcggcagcc aggcgctcga agccatgacc cgccagaacc tggcgatgtt
     5581 cgaacgcacg atgcggatgt tctcgccgtt cggcattcag gcggcacgcg aagccgagtc
     5641 ccgctcaagc aacggttctg cggaatccaa gcccgcctcg tcagacgaag tgagcgcgct
     5701 caagagcgag atcgaggcga tgcgccggca actcgccgaa attgcccgcg accgaaaata
     5761 gttggtatta cgcggcctcg agacgcttca gttccgcgtc gaactcaagg cacggttgct
     5821 ctgcctcttc tcacggaagc cagtggatca tctgcgggaa gatcgtgcgg tacaaagcga
     5881 ccgtcttggg tttccacggc agattgtctg ctgcgcgtgc ttcgccaaga atctgttgtc
     5941 ggtgcgccct taccctgtca ggatttgctg cataggtgcg ttcgggggcg tcttcgaaac
     6001 cctcaggttc cgcatcggac agaagcagat catattgacg cgtcatgatg cttggccgac
     6061 tcgctctcgc aatcatgcgc cgccaggccg ctccaagcga gagggcaatc ggtgaatccg
     6121 gggacatgac attccgttat ctgccgcaga aggctctcgg ctagagcgga cttctcatct
     6181 tgaaccaaac atgtcattcc cgccgaagtg ggaatgacat cggttggctg attcaacccg
     6241 cgctcttcca cggaggcgcg agcgtgggtt cgccgaaatg gtatccctgg aagtagtcga
     6301 ctccgaagct cgcgagaagc gccgcttcct cctcggaacc gacccattcc gccaccgtct
     6361 tgagccggaa attctttgcc aggtccacga gcgtgcgcac gaatatctgg ttctccggcg
     6421 acgtggagag gccgcgcaca aacgcgccgt cgatcttcac gatgtcgacg cgcagcatct
     6481 gcaggttgcg gaacgaggtg aagcccgcgc caaaatcgtc gattgcgacc ttgcagccca
     6541 gctcccgcag attcgaaatg aatcgcgagc tctcctcgaa atcattaagc gcggccgttt
     6601 ccgtaagctc gacgatgatg cgccgggcga cgccgttgtt ggcgcggacg tagtggatga
     6661 aggaggtgag ccacgaagga tcggtggcgg tcgtgcccga cacgttcacg gcgagcgaga
     6721 tttccggata ggcgtgcagc tcggcaatgg ccatttcaag cgcatggcgg tcgacctggc
     6781 gcacgagtcc cagctgctcg gctgccggaa tgaagtgccc agccgtggca atcgagccgt
     6841 cggcgcgcac catcctgagc agacattcgt gatgcaccgg ccggcgctcc cgcgcgcaga
     6901 caatcggctg atatgcgaac acgagcctct tggtctcgag cgcctcggcc acctcgtcgg
     6961 caaccgccat cagccgaagg cgcgccatct cgcgctgcgg cgatttcgta tagatccaga
     7021 agccgttccg gccggcaccg cgcgcgcggt cgagcgcttc ttccgcacgc agcatggctt
     7081 cctgacctga agacgcgtcg gaaggcaacc acacggcgcc catgctgatc gtcgccgaaa
     7141 ccgggccccg ccgtgtcgca atcacttcgc cgcgaaccgc gtcgcgcatg cgttctgcgg
     7201 ccaacgccat ctcggcttcc gtgcacgggc cgagaatgat tccaagcttg ttgccggcga
     7261 tccggccgat cacgtcgctc gcgcgcaggg cggacgcgag ccgctcgccg accgtgacga
     7321 tcacctcgtc cgcggcgcca aatccgtaag cttcattgat gactgcgagc ctgtcgatcg
     7381 cggcaaccag ataggcgcag ttcgtcgcgc cggcttttga aatctcgatt gcacgggcaa
     7441 tctccgacct caggctcgtg cggttgagat gaccggtgag ttcgtcgcgc gtggcaagat
     7501 aggtcagtct ctgcgcttcg cgcttgcgtt cggtgatgac gcgcaacacg ccggcaacgc
     7561 gctcggcctg gcccgccggt cccggtatgc gcacgccgcg cagttcgagc cacaccgtcc
     7621 ccatcgcgga cgaaacttcg aattcgattt cgaagaaatt atcttcgagg cttgcctgcc
     7681 gcaggatcgc atcgatgcgc tcacggccgg catggctcat ccaggcgcgc aattccgcgc
     7741 cgcggctcat gcgggccggt tccgaatgaa gcgccagcag atcgattgcg ccgtcccagt
     7801 cgacgcggtc gtcggcgatg gtccagtcga acgcggcttc gccggcgccg cgcgccgcaa
     7861 gcctcaggcg ttcgaggtct tcgatcgcgg tgagagcgcg aatcccgtcg acagatcttt
     7921 tgggaagtgt ctgaaacgcc acaatccacc gtccccggtc gttttcggcc tttggacgcg
     7981 catgatcggg gatgggtcgt taaaaaatgg cgaaattttc agtgttttcg ggacgatagt
     8041 gccggtcgtg cccaggtttc gaatgttgcg cctcgccctg tcatgttttc gccgtcacgc
     8101 gcgctgtttc attttggaac atcgcgcgac gccatgaccg atcggcattc actgtgacaa
     8161 gccgatatag cccgcaccga ttatgcggcc gtacccgccg tcctgcacca gaaccgcctc
     8221 cgcgtcgtgc ggcgcgctga tcatgtcgct gcggggcagg tcgagggaaa gtgcctggcc
     8281 cttccagacg ccgatcgctt tcacgtcgcg cacgatattc cgatagccga tactgcggcc
     8341 ttcgttctcg cccgaggcga tatgcacagt cgtcctgctc agaatatgga aaatccaaat
     8401 tgtcgcttcg tcgccgttgc gctgtgcgcc cgcaccgatc gcgatgtgga gcgtcgccgg
     8461 cgtggcgctc aaggttactg gcactgcgcg catgtcggcc gtgcgtgcgg caatcgccga
     8521 tgccacctgg gcttcgcggc tccccaccac gtcgctggca ccgtcgacga tcatctgcgg
     8581 cgtatagatg ccgccgcggc ccatcgcctg tgcgtaggct ttctgccggc gcgtgttcgc
     8641 ttcgctggcg agcgtgtctt tccagcccag catgtcccaa taggtcaccg ggagactgag
     8701 ggcgagcacg tccttgcggt ccgcgagccg gccgaggagc gcatctgccg gcgggcagga
     8761 actgcagccc tgactggtga acagctccag cacgacctgg cgctttggtc ccgccattga
     8821 cgaattgccg gccgcgagcg cggtcgccgc gagtgctgtc gctacaattg ttgcaaaccg
     8881 cgtcatcggc ccaacatagg gccccccgac tctgccctcc agtcacttcg gcttgagacc
     8941 gtgccgggac gacctcggcg caaattcgct ctgaaatcac gacgcgttcc gcacgaaagt
     9001 tgcggtaaca gcgtggtaac cagctctacg cggccagcag gttcctgaga acgaacggca
     9061 gtatcccgcc attctcgaaa taggccactt cgtcggcggt atcgatgcgc aggatcagcg
     9121 gcaggtcggc tttacggcca tcgggatagg cgatggtggc gatcagccgc atggcgggcg
     9181 caatccggcc ggacaatcca cgaatgtcga ttgcctcccg accgctcagt ccgaagtcgc
     9241 gccgggtttt cccgtccgca aacacgaacg gcgcaacctc catgccgatc aggttggagc
     9301 ggtggatgcg ctcgaagctt tccgcgatga cggccttcac acccaacagc atggtgcctt
     9361 tcgccgccca gtcgcgtgag gatcccgtgc cgtattcgcg gccggcgaga atgacgagcg
     9421 gcgtaccgtc cttcttgt
//
LOCUS       DASZMC010000184         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1186172,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000184 DASZMC010000000
VERSION     DASZMC010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186172"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VG274_03660"
     CDS             <1..500
                     /locus_tag="VG274_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWE05777.1"
                     /translation="AGLLKERGVPLLIHQPSYSMLNRWVEGGLLSALEETGTGCIAFS
                     PLAQGLLTDKYLGGIKEGTRAAENKYFAKELITDENLACVRALHGIAQARGQSLAQMA
                     IAWVLRNPGVTSALIGARNVAQLDNALDATKNLTFTAAELAEIDQHAREGGIDLWREV
                     SSFEG"
     gene            622..>1522
                     /locus_tag="VG274_03665"
     CDS             622..>1522
                     /locus_tag="VG274_03665"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWE05778.1"
                     /translation="MNAEPRDGQEPPYAVGATVSLQERQYRTLKNGDMFGLFDLSGNI
                     LPYEGGTEGLYNRDTRYLSVFELSIGGGRPVLLSSTLRDDNTALICDLSNPDLSRDGQ
                     LVIEHGVIHIRRTRFLINATLFERIQVRSFARDAQRVRLEFAFDSDFADLFEVRGTPR
                     TRHGTREAAVVEADRVTLGYLGLDGKRRSTVLRFSPLPSALDGSRAQFDLTLEPDRQS
                     ILYLEIACDGEAPPPVRDVREHYLASLVEARRWMRTRSARAAAMESSNELFNEAIRRA
                     VADLYMLITEKPTGLYPYAGIPWF"
BASE COUNT          267 a          556 c          452 g          247 t
ORIGIN      
        1 ccgctggcct gctgaaggaa cgcggcgtac cactgctcat ccaccagccc tcctattcca
       61 tgctcaaccg ctgggtcgaa ggcgggctgc tgtcagcgct ggaggagacc ggcaccggct
      121 gcatcgcctt ctcacccctc gcccaggggt tgctgaccga caaatatctc ggcggcatca
      181 aggaaggcac gcgcgcggcg gagaataagt atttcgccaa ggaactcatc accgacgaga
      241 acctcgcctg cgttcgcgcc ctccacggaa tcgcgcaggc gcgcggccag tccctcgccc
      301 agatggcgat cgcctgggtg ctccgcaacc ccggcgtcac cagtgcgctg atcggcgcgc
      361 gcaacgtcgc ccagctcgac aatgcgctgg acgcgaccaa gaacctcacc ttcacggcgg
      421 ctgaactggc cgagatcgac cagcatgcgc gcgaaggcgg catcgacctc tggcgcgagg
      481 tttcgtcctt cgagggctaa aatgacctat catccccggg agaacgccct tccccgggga
      541 tgacaaggcc cgatcacgga ccagatcctg gtccgcacgc cccaggccat cataccccag
      601 gccatacgga gatcgcccga cgtgaacgcc gaaccccgag acggccaaga accgccctat
      661 gccgtgggcg ccacggtgtc gctgcaggag cggcaatacc ggacgctcaa aaacggcgac
      721 atgttcggcc tcttcgatct ctcgggcaat atcctgccct atgagggcgg cacggaggga
      781 ctctacaacc gcgacacccg gtatctctcg gtcttcgagc tttccatcgg cggcggccgc
      841 ccggttctgc tcagctccac gctgcgcgac gacaataccg cgctgatctg cgatctctcc
      901 aatcccgacc tctcgcggga cggccagttg gtgatcgagc atggcgtgat ccacatccgc
      961 cgcacgcgtt tcctgatcaa tgcaaccttg ttcgagcgca tccaggtccg cagcttcgcg
     1021 cgagatgccc agcgcgtgcg cctggaattc gccttcgatt ccgatttcgc cgatctcttc
     1081 gaggtgcgcg gcacgccgcg cacgcggcac ggcacgcgcg aggcggccgt ggtcgaagcc
     1141 gaccgcgtca cgctgggcta cctcgggctc gacggcaagc gccgcagcac ggtgctccgc
     1201 ttcagcccgc ttccttcggc gctcgacggc agccgcgcgc agttcgacct gacgctggaa
     1261 cccgaccggc agtccatcct ctatctcgag atcgcctgcg atggcgaggc gcccccgccg
     1321 gtacgggatg tgcgcgagca ctacctcgct tccctggtcg aggcgcggcg ctggatgcgc
     1381 acccgctcgg cgcgggccgc cgccatggaa agctcgaacg aactcttcaa cgaggcaatc
     1441 cgccgcgcgg tcgccgatct ctatatgctg atcaccgaga agccgaccgg cctctacccc
     1501 tatgccggca ttccctggtt ca
//
LOCUS       DASZMC010000185         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4467222,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000185 DASZMC010000000
VERSION     DASZMC010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4467222"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VG274_03670"
     CDS             complement(<1..749)
                     /locus_tag="VG274_03670"
                     /inference="COORDINATES: protein motif:HMM:NF017931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HWE05779.1"
                     /translation="MIGLEEVYLFAGFVFAGFAVQSARDRSNPRRFGNAAFWGLFCAS
                     FVLGSHLPDAANGALALALVLIGGFRALGKGGEQTSSEADRRASATEYGNALFVPALI
                     IPTTALLGTLALKNTAPGGMPFIDAKQATLISLALGVVIAWIVALLWLRPRLLAPLDE
                     GRRLMDTIGWAAVLPQMLASLGAVFALAGVGRAVGLVFAHYIPFDHAFAAIAAYCIGM
                     AVFTAIMGNAFAAFPVMTAAIGLPLVVHKFGG"
     gene            complement(746..1435)
                     /locus_tag="VG274_03675"
     CDS             complement(746..1435)
                     /locus_tag="VG274_03675"
                     /inference="COORDINATES: protein motif:HMM:NF017916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HWE05780.1"
                     /translation="MRTLLGVGVVVLGFLIRINPLLVIAAAAFVTGWAAGFGPVAILA
                     AFGKAFNESRYVSLVFLVLPVIGLLERYGLQERARMLVARIAAATPGRLLVAYLALRQ
                     VFSAVGLVSIGGQAQMVRPLIAPMAEAAGEGRGAELSGRARFRIRAYAASADNVGAFF
                     GEDVFIAIGSILLIKGFLEQSGVVVQPIELSLWAIPTALTALAIHGTRLLLLDRWLTA
                     QAAVVPSESSP"
     gene            1687..2871
                     /locus_tag="VG274_03680"
     CDS             1687..2871
                     /locus_tag="VG274_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HWE05781.1"
                     /translation="MPMKLVETSSSLDPVALPLPTAVVARTAVATVRERLVSLDAMRG
                     FAVIGMIVVNALASSKDAYGFQPDYSFLSHSPWAGFTFADFVFPAFVFITGFSVAASL
                     RRARLDWQVLRRIGARTVGLMALGFLLTNITWFGNMDHGDWRLPGVLQRIGICYFATA
                     ILFVACGPRARLVLALLVLLVYWPLTLAPIPGHATNLLLPGANFASFIDRILLGSHAL
                     VTGPGGYDPEGLLSTLPAIAQCLLGALVGNWLLNNRAVERAPWKLAAAGVLTLALGLA
                     WSPFFPLVKNIWSSSYVLFSTGLAMLLLALSWWGIDRKRLNFWGVTFLEAFGVNALLA
                     YALQQTAQLLPAGDDMHAIGAASLKMEGSALIANLPILLFLVMLWVPLEFMRRRRWIV
                     RL"
     gene            complement(2876..4024)
                     /locus_tag="VG274_03685"
     CDS             complement(2876..4024)
                     /locus_tag="VG274_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05782.1"
                     /translation="MSNSGQSVQISRLDVEKSAADWLAVPFAVYANDPDWVPQLNLLE
                     KQRISKKHAPFFTFGEAAFFVASRSGKPVGRITAHVNRRHLERHRDNTGHFGFFDCAD
                     DREAARALVETAADWLKTQRLVRMMGPLSFSINEECGCLVSGFESPPAMLMPHGKPWM
                     GPLLEGAGLAKVIDLFAYRTTPRRLPPRIEQLAARAERFGSTQLRNFDMRRYRAEIDL
                     LIDIYNDAWSDAWGFVPFSTAEIDSLAKELRPFFRNEYGRFLMIDGREVGFAVALPDL
                     NGIIAPFRGRLFPFNWAKLIWTMKRETWRTARIPFLGIRRAWRTTPRGSALVILLVKS
                     LVIQASTRYQLDWAEYSWIRENDSRMVALGEAIAGPPAKTYRIYAKPI"
BASE COUNT          808 a         1322 c         1281 g          772 t
ORIGIN      
        1 ccgccgaatt tgtggacgac cagcggcaga ccaattgctg cggtcatcac cggaaacgca
       61 gcgaacgcat tgcccatgat cgcggtgaag accgccatgc cgatgcaata ggcggcgatg
      121 gcggcaaagg cgtgatcgaa agggatgtaa tgggcgaaca ccagtcccac ggcgcggccc
      181 acgcctgcca gcgcaaacac cgcgccgaga gaggccagca tctgcggcag aaccgcggcc
      241 cagccgatcg tgtccatcag gcgcctgccc tcgtcgagcg gcgccagaag tcgcggccga
      301 agccagagaa gcgcgacaat ccaggcgatc acgacaccaa gcgcgagcga gatcaacgtc
      361 gcctgctttg cgtcgatgaa tggcatgcct cccggagcgg tattcttcag cgccagcgtt
      421 ccaaggagtg ccgttgtggg aatgatgagc gcgggaacga acagcgcgtt gccgtactcc
      481 gtggcgctcg cgcggcgatc ggcttcgctt gaagtttgct cgccaccttt gccaagagca
      541 cggaaaccgc cgataagcac cagcgcaagg gcgagcgctc catttgcggc gtcgggaaga
      601 tgcgagccga ggacaaaact cgcgcagaac agcccccaga acgcggcgtt tccaaacctc
      661 ctgggattgc tgcggtcgcg cgcgctctgc accgcaaagc ccgcgaaaac aaagcccgcg
      721 aacagataaa cttcttccag gccgatcacg gcgagctttc cgacggcacg acggcagctt
      781 gcgccgtgag ccaacggtcg agaagcagca aacgcgtgcc gtggatcgcg agtgccgtga
      841 gcgccgtcgg aatggcccag agcgaaagct cgatcggctg aaccacgaca ccgctttgct
      901 cgagaaaacc cttgatcagg agaatagaac cgatggcgat aaaaacgtct tcgccgaaaa
      961 aagcgccgac attgtcggcg gaggcggcgt aggcacggat gcggaagcgt gccctgccgc
     1021 tcaattccgc cccgcgcccc tcacccgcag cttccgccat gggcgcgatc agcggccgga
     1081 ccatttgcgc ttgccctccg atcgagacga ggcccacggc agaaaacacc tgacgcagcg
     1141 ccagatatgc aaccagcagg cgtccgggcg tcgcggcagc gatgcgcgcg acgagcatgc
     1201 gcgcgcgctc ctgcaatccg tagcgctcga gcaatccgat cacgggcaga accagaaata
     1261 cgaggctgac atagcggctc tcgttgaacg ccttcccgaa ggccgccaga attgcgaccg
     1321 gaccaaaccc ggccgcccat ccggtcacaa atgccgccgc cgcgatgacc agcaacggat
     1381 tgatgcggat cagaaatccc aacaccacga caccgacgcc cagcagtgtc cgcatgagtc
     1441 accttcccgc ttcgaatcag aaactgccgc tgaaaaacga tcttttgcga ttgcatttcg
     1501 agttgcgcgc agacattgtg attcgctttc gtgccccgca agttctcatt tgttaaacaa
     1561 gtgcgcgcta ttgttccatg agcgtgtacg acgctgcccg acagcgcgag gcttatcgaa
     1621 ggcgctatcc ttcactgcgc tgcctctcgc cgcccgcggg ttgcacgccg aacggcaagc
     1681 agaggcatgc caatgaaatt ggtggaaacg agcagcagcc tcgatccggt cgcgctaccc
     1741 ctgccgacgg cggtcgtcgc gcggaccgca gttgcgaccg tgcgcgagcg gctcgtctcg
     1801 ctcgacgcga tgcgcggctt cgccgtcatc ggcatgatcg tcgtaaacgc gcttgcctct
     1861 tcgaaggacg cctacggctt tcaaccggac tattcgtttc tgtcgcactc gccctgggcg
     1921 ggattcactt ttgccgattt cgtttttccc gccttcgtct tcatcacggg attctccgtg
     1981 gccgcatcct tgcgccgcgc gcgcctcgat tggcaggtgc tgcgtcgaat cggagcgcgg
     2041 acggtggggt tgatggcgct cgggtttctg ctgaccaata tcacctggtt cggcaatatg
     2101 gatcacggtg actggcgttt gcctggtgtg ttgcaacgca tcggcatctg ctacttcgca
     2161 acagcgatcc tcttcgtcgc gtgcggacca cgcgcgcggc tcgtgctggc gttgctcgtt
     2221 ctgcttgtct attggccgct gacgctcgcc ccgattcccg gccacgccac aaaccttctc
     2281 cttcccggag caaacttcgc ctccttcatc gatcgcatac tccttggttc gcatgcgctc
     2341 gtgacgggcc ctggcggata cgatcccgaa ggtctgctga gcacgttgcc ggcaattgcc
     2401 caatgcctgc tcggtgcgct tgtcggcaat tggttgttga acaaccgtgc ggtcgaacgt
     2461 gcaccgtgga aactcgccgc ggccggcgtc ttaacgcttg ccctcggcct cgcctggagt
     2521 ccgttttttc cgctcgtcaa gaacatctgg tccagcagct atgttctctt ttccaccgga
     2581 cttgcgatgt tgcttcttgc cctgtcctgg tgggggattg accgcaaacg gctcaacttc
     2641 tggggcgtga cattcctcga agcgttcggc gtcaacgcgc tgctcgccta cgccctgcag
     2701 caaacggcgc agcttctgcc ggcgggggat gatatgcacg caatcggcgc tgcaagcctc
     2761 aagatggaag gatccgctct gatcgcaaac cttccgatat tgctcttcct cgtcatgctc
     2821 tgggtgcccc tcgagttcat gcgccggcgg cggtggatcg tcaggctctg acgcgtcaga
     2881 tcggttttgc gtagatgcgg taagtcttgg cgggcgggcc ggcaatcgct tcgccgagcg
     2941 cgaccatccg ggaatcattt tcacgaatcc aggaatactc ggcccagtcg agctggtagc
     3001 gcgtcgaagc ctggatcacg agcgatttta caagcaggat gacgagcgcg ctgccgcgcg
     3061 gcgtggtgcg ccaggcgcgc cgaatgccga ggaaggggat gcgtgcggtc cgccaggtct
     3121 cgcgcttcat cgtccagatc agcttcgccc aattgaaggg aaagaggcga ccacggaacg
     3181 gcgcgatgat gccgttgaga tccggcaggg ccacggcaaa gccgacctcc cggccatcga
     3241 tcatcaggaa gcggccatat tcgttgcgaa agaagggccg caattctttg gccagcgagt
     3301 cgatctccgc ggtgctgaag ggaacgaagc cccaggcgtc gctccacgcg tcgttgtaga
     3361 tgtcgatgag aagatcgatc tccgcgcgat agcggcgcat gtcgaaattg cgcaattgcg
     3421 tgctgccgaa tcgctcggcg cgcgccgcaa gctgctcgat gcgcggcggc aatcggcgcg
     3481 gcgtcgtgcg gtaggcgaaa agatcgatta ccttggcgag accggcacct tcaagcaacg
     3541 gccccatcca tggtttgccg tgaggcatga gcatggccgg cggcgattcg aagccggaga
     3601 cgaggcagcc gcattcttcg ttgatggaga aactgagcgg ccccatcatt cgtacgaggc
     3661 gctgcgtctt cagccagtcc gccgcggtct cgacgagcgc gcgcgccgct tcgcgatcgt
     3721 ctgcgcagtc gaaaaaacca aaatggccgg tgttgtcgcg atgacgctcg agatgacgac
     3781 gattgacatg cgcggtgatg cggccaaccg gcttgccgct ccgcgaagcg acaaagaaag
     3841 ccgcttcgcc gaaagtgaag aacggcgcat gcttcttcga aattcgctgc ttttcgagca
     3901 ggttgagctg gggaacccaa tcgggatcgt tcgcgtagac ggcaaaaggc acggcgagcc
     3961 agtcggcggc gcttttttca acatcgagcc gggaaatctg cacgctctgt ccggaattgc
     4021 tcatgtgcgg cctctccgcg tgcgctgaag aagcggatcg atcagcgacg gtggcaacgc
     4081 tgccgcaaat cttgtcagcg ccgcgagttg ccatgggaaa ctgatttccc gcttgccgcg
     4141 tccaacctcg gtgacgattc gcgccgcggc ccgctcggcg ctc
//
LOCUS       DASZMC010000186         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5296045,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000186 DASZMC010000000
VERSION     DASZMC010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5296045"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..717
                     /locus_tag="VG274_03690"
     CDS             52..717
                     /locus_tag="VG274_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HWE05783.1"
                     /translation="MDGDGAGEGRSGKIESRSGKNASTGGELDSSDGGRESSRGRNAD
                     RGGNKEKNGGGSKEKGPPPPLAGGGRGEGAVGSPVRPSILAHARSMRREQTPAERRLW
                     QGLRNHQLGDLKFRRQVPLGHFIADFYCPTAKLVVELDGVSHIDSQTDGARDAWMLSQ
                     GLRVLRFSNHEVLSNPEGVLLAMKQAALSAMPAQLQPPPPPNPLPQGEGESSRIPPFP
                     THV"
     gene            710..2056
                     /locus_tag="VG274_03695"
     CDS             710..2056
                     /locus_tag="VG274_03695"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HWE05784.1"
                     /translation="MSDRSSLFGEIRRMARTSGAVGGIAARVAGSRMFGIRTDRAAHA
                     EDLRVILGGLKGPLMKVAQFLSTVPDALPMEYAEELAQLQSNAPAMGWNFVRRRMASE
                     LGPDWQSRFTTFIQTAAAAASLGQVHRASLPDGTEVACKLQYPDMPSVVEADLRQLRL
                     AMAIYHRMDKAINQDEVYKELAERLREELDYEREAAHMRLYGLMLAEHPVVHVPVPFA
                     AFSTKRLLTMTWLDGRPLMQRLAEDPPQDERNRIAEALFQAWYVPFYRFGVIHGDPHL
                     GNYQVRPDASVNLLDYGAVRVFDAKFVRGVIDLFEAVRDGDDAKAHHAYETWGFTNLS
                     HETMDVLNLWARFLYEPLTDDRVRRIQDTDDPMFGRAVAEQVHRGLKRTGGVKPPREF
                     VLMDRSAIGLGGVFLRLKAELNWSRLFHELIEDFDSAALAERQARALAEARVPAAG"
BASE COUNT          388 a          638 c          710 g          345 t
ORIGIN      
        1 gatcgccggg ttcatcgatg agttggagga agcggggtga ggacggggga aatggacggg
       61 gatggggctg gcgagggtag gagcgggaag atagagtcca ggagcggaaa gaacgcgagc
      121 actggcggcg agctagatag cagtgatggc gggagggaga gcagccgcgg gaggaacgcg
      181 gacaggggcg ggaacaagga aaagaacggg ggcgggagca aggaaaaagg acctccccct
      241 ccccttgcgg gagggggccg gggggagggg gcggtcgggt cacccgtacg cccatcgatc
      301 ctcgcccacg cgcgcagcat gcgtcgcgag cagacacctg cggagcggcg tctctggcag
      361 ggcctgcgta accatcagtt gggcgatcta aagttccgac ggcaggtgcc gctcggccat
      421 ttcattgcgg atttctactg cccaacagcg aaactggtgg tcgaactgga cggcgtctcg
      481 catatcgatt cccaaactga tggcgctcgg gacgcgtgga tgctatcaca gggtcttcgc
      541 gtgctcaggt tctcgaacca cgaagtgctg agcaacccag aaggagtgct gctcgccatg
      601 aagcaagcgg cgctttctgc tatgccggct cagctccagc cgccccctcc ccccaacccc
      661 ctcccgcaag gggaggggga gtcgtcccgt atccccccgt tccccaccca tgtctgaccg
      721 cagcagcctg ttcggcgaaa tccgccgaat ggcccgcacc tccggcgccg tcggcggcat
      781 cgcggcgcgg gttgccgggt cacggatgtt cggcatcagg accgaccggg ccgcccatgc
      841 cgaggatctc agggtcatcc tgggcgggct gaaggggccg ctgatgaagg tggctcagtt
      901 cctgtccacc gtgcctgacg cgctgcccat ggaatacgcc gaggaattgg cgcaactcca
      961 gtccaacgcc cccgccatgg gctggaactt cgtccgccgc cgcatggcga gtgaactcgg
     1021 acctgactgg cagtcccgct tcaccacctt catccagacc gccgctgccg ccgcctcgct
     1081 cggccaggtc caccgcgcca gcctgcccga cggcaccgag gtggcctgca agcttcagta
     1141 cccggatatg cccagcgtgg tagaggccga tttgcgccaa ctgcggctcg cgatggcgat
     1201 ctatcaccgc atggacaagg cgatcaacca ggacgaggtc tataaagagc tcgccgaacg
     1261 tttgcgcgaa gaactggact acgaacgcga agcggcgcat atgcggttgt atggactgat
     1321 gctcgctgag catccggtgg tccacgtgcc ggtgccgttc gcggccttca gcaccaagcg
     1381 gcttttgacc atgacctggc tggatggccg cccgttgatg cagcggctgg ccgaggaccc
     1441 gccgcaagac gagcgcaacc gaatcgcaga agcattgttc caagcgtggt acgtgccctt
     1501 ctaccggttc ggcgtgatcc acggcgaccc tcatctgggc aattaccagg tgcgcccaga
     1561 cgcctcggtc aacctgctgg actacggcgc ggtccgtgtg ttcgacgcaa agttcgtccg
     1621 cggcgtgatc gatctgttcg aggcggtgcg cgatggcgac gatgccaagg cgcatcatgc
     1681 gtacgaaacc tggggcttta ccaacctgtc gcatgaaacg atggatgtgc tcaacctttg
     1741 ggcacgcttc ctgtatgagc cgctgaccga cgatcgggtg cggcggatcc aggacaccga
     1801 tgacccgatg ttcgggcgcg cggtggcgga gcaggtacat cgcggcctga agcgcaccgg
     1861 cggagtaaag ccgccgcgcg agttcgtcct catggaccgc tctgcgatcg gtctaggcgg
     1921 tgtattcctg cggttgaagg ctgagttgaa ctggagtcgg ctgttccacg agctgatcga
     1981 ggatttcgac tcggcagcgc tggcggagcg tcaagccaga gcattggccg aggcccgggt
     2041 accggcggcg gggtagagac ccattcgtcc caagatcgag g
//
LOCUS       DASZMC010000187         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2832987,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000187 DASZMC010000000
VERSION     DASZMC010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832987"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VG274_03700"
     CDS             <1..815
                     /locus_tag="VG274_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWE05785.1"
                     /translation="GGMIRPISMIPDQEDTVDLAIRSHVIINGLDAPGLIASVRQIPL
                     QQRVARTETLWEMADGTGGQFVHDTNDLFGGFERLAAAPEVTYLLGFSPQNLKLNGKF
                     HTLKVKVRPAGLSVQARTGYRAPTHLASAAETASEEIREAVFSRGEIKDIGIDIETEV
                     SRRIPAEATLSVVSRIDVSQLHYRKADGRNIDDIELVCALFDRNGVYIDSLIRTIRLR
                     LLDDTLRNRVGAGIPIRSDFKVPPGTYAIRVVARDSEGAMMAAQNGAAVIPR"
     gene            complement(837..>1502)
                     /locus_tag="VG274_03705"
     CDS             complement(837..>1502)
                     /locus_tag="VG274_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HWE05786.1"
                     /translation="KARERAGVAEDDLLLLATWAGEAVGHRPEETVYQACGQLRLQAA
                     QKYAEKHGQFNASRYEFLWVTNFPMFEWNVEDNRWVAAHHPFTSVNDEDLEKLESDPA
                     RCRARSYDLVLNGVELGSGSIRIHRRDVQAKVFAALGFSDEEARRRFGFLLDALEYGA
                     PPHGGIALGLDRLVMILAGESSIRDVIPFPKTAKGTDLMCEAPAPVPERQLRELGIAL
                     RKQ"
BASE COUNT          286 a          501 c          450 g          265 t
ORIGIN      
        1 cgggaggcat gatccggccg atttcgatga tccccgacca ggaggacact gtcgatctcg
       61 ccattcgctc tcatgtgatc atcaacggcc tcgacgcgcc ggggctcatc gccagcgtca
      121 gacagatacc gctgcaacaa agggttgccc ggaccgagac cctttgggag atggccgatg
      181 ggaccggcgg ccagtttgtt cacgatacca acgacctgtt cggcggcttc gagaggctcg
      241 ccgccgcgcc tgaggtcacc tacctgctcg gcttctcgcc gcagaacctt aagctgaacg
      301 gcaaattcca caccctcaag gtcaaagtga ggcccgccgg actgagtgtg caggcgcgca
      361 ccggatacag ggctcccacc catctcgcga gcgccgcgga aaccgccagc gaagagatcc
      421 gcgaagcggt attctcacgc ggagagatca aagacatcgg aatcgacatc gagaccgagg
      481 tttccagacg cattccggcg gaagccactt tgtcggtggt gtcgcgcatc gacgtcagcc
      541 agttgcatta ccgcaaggcg gatggccgga acatcgacga tatcgagctg gtctgcgcgc
      601 tgttcgaccg caacggcgtc tatatcgata gcctcattag aacgatcagg ctgcggctgc
      661 tcgacgacac gctgcggaac cgcgtgggcg ccggaattcc catccgcagc gatttcaaag
      721 tgccgccggg aacgtacgcc atccgcgtgg tggcgcgcga ttccgaaggt gcaatgatgg
      781 cggcgcaaaa cggcgcggcg gtgatccccc gttgacgggc ttctggcgca gccgtcttac
      841 tgcttccgca gcgcgatccc cagctcgcgc aactgccgct ccggaaccgg cgccggagct
      901 tcgcacatca ggtccgtgcc cttcgccgtt ttcgggaacg ggatcacgtc tcgaatggag
      961 ctttcgcccg cgaggatcat caccagccga tcgagaccca gcgcgatccc gccatgtgga
     1021 ggagcgccgt attcgagcgc gtccagcaga aatccgaacc gccggcgcgc ttcctcgtcg
     1081 ctgaacccga gggccgcgaa caccttcgcc tgcacgtcgc ggcggtgaat acgaatcgag
     1141 ccggacccga gttcgacgcc attcaggacg agatcgtagg aacgggcgcg gcatcgcgcg
     1201 gggtcgctct caagcttttc cagatcctcg tcgttcacgg aagtgaacgg gtgatgcgcg
     1261 gccacccaac gattatcctc gacgttccat tcaaacatcg gaaagttcgt tacccacagg
     1321 aattcgtagc ggctcgcgtt gaactgcccg tgcttctccg cgtatttttg cgcggcctgc
     1381 aggcgcaact gtccgcacgc ctgatacacc gtctcttccg gacgatgtcc cacggcttcg
     1441 ccggcccagg tcgccagcag cagcagatcg tcttcggcca cgcccgcgcg ctcccgcgcc
     1501 tt
//
LOCUS       DASZMC010000188         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_363656,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000188 DASZMC010000000
VERSION     DASZMC010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363656"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VG274_03710"
     CDS             complement(<1..1155)
                     /locus_tag="VG274_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05787.1"
                     /translation="MLKAAEANDPDARRALLNHTVDAARAAWAARFETDSGSEQVSNF
                     LGHPQLPLFAGFAAIAILAVASGGFSATRELAARLPYTDPGRLVVLAQGPPVFGMRLG
                     FREVETELFRRKSQALSGLAAYSWHNGTIVTLRGNRSIRLANVDPSFFEVLGVAAPTS
                     RLADDEFFVSRDFWRGALRADPNLKGRTYLVDGRPMRLAGVLPANFEFLSAPIAIWTS
                     RPEPAPSPLLPRRLWWVNLRGIVARLAPGVMPSTAVKELRDLQVRFSLARPNYIVQAT
                     PIEYLMYRNLQSYTWYLMSLLAALLLLAAARTVIDTRRGLPPRRSARYWGFFALKTVL
                     PLTALYFAILEFTPATELGLTGGDATRGGPMLLWTSFASVVVIAIWAWRDQ"
     gene            complement(1365..2144)
                     /locus_tag="VG274_03715"
     CDS             complement(1365..2144)
                     /locus_tag="VG274_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE05788.1"
                     /translation="MKPLTGKIAVITGSNRGIGRSIAQRLSREGANVVLAARDASVLS
                     EAADEIREAGGRAETIALDLRMPEACAQLVDFAVAKFGRIDIVVNNAGATKLGDFTEL
                     TDADWADGFALKFFAAVRVTRAAWPHLKASGGSVVNISGIGGRTPGPMFAIGGSVNAA
                     MNCLTKSLADTGVRDKIRVNGILPGYVRTDRLKHRLDAVVKDTGVALSQAEERLTADA
                     KTLQIGEPEDIASLVAFIVGPEGRFFHGALIDMDGGATKAV"
     gene            complement(2164..3027)
                     /locus_tag="VG274_03720"
     CDS             complement(2164..3027)
                     /locus_tag="VG274_03720"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWE05789.1"
                     /translation="MNWKPEPEFGGFYAAPYMQHGLNVDILPGGAGTPSVQMIGAGSA
                     EFGIVEADEIVVARARGNDVVALYAAFQHSPAALMVHASRKFADIGDIFKKGGTLAIQ
                     KGLPFARLLERQYGFDKVQVVPSPGGDISAFLRDPKFVQQVYMTSEYLTAKRQGSDAQ
                     VFAISAAGFDPYNTVMATSGDYLRRNPETAKAMVAAVREGWRAYLDDPQATNQRMNQL
                     NPSMSLAGFAETAQAQKPLIETDETRKNGLGCMTRERWLTLISQLEGLGDLPKVPAPE
                     DCFTTLGSTNS"
     gene            complement(3176..>3942)
                     /locus_tag="VG274_03725"
     CDS             complement(3176..>3942)
                     /locus_tag="VG274_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWE05790.1"
                     /translation="GVCVSRLKSIIPALIPFVVLTASVELAVRQGWVHSYLVPAPSQV
                     LRAMIDSRDELFTAVASTSASALLGFALSTIGGIAVAVFLSSSRPIQRAFYPYAVFFQ
                     TVPIIAIAPLLVIWFGYGMRTVVASAFVVSIFPVIANTLTGILSTEPPLRDLFRLYGA
                     STWTTLVKLRFPSALPQILTGLRVAAGLAVIGAIVGEFIGGQGLGSVVDVARTQQRVD
                     KVFAAVLLASVLGLALFGLINLVSYFTLRRWHASEQ"
BASE COUNT          815 a         1121 c         1362 g          644 t
ORIGIN      
        1 ctgatcccgc caggcccaga tggcaatcac gacgaccgag gcgaaggacg tccacagcag
       61 catgggaccg ccgcgcgtgg cgtcgccacc ggtgagcccg agctcggttg cgggggtgaa
      121 ttcgaggatc gcgaaataca acgcggtcag cggcagaacg gtcttaagcg caaaaaatcc
      181 ccagtagcgg gcggagcggc gcgggggcaa gccacggcgg gtgtcgatga cggtgcgcgc
      241 ggcggccagc aggagcagcg cggcgagcag cgacatcaag taccaggtat aggattgcag
      301 attgcgatac atcaggtatt cgatgggcgt ggcttgcacg atgtagttcg ggcgcgcgag
      361 gctgaaacgg acttgcagat cgcggagttc ctttacagca gtggacggca tgacacccgg
      421 cgcgaggcgc gcaacgatgc cgcgcaaatt cacccaccat aagcggcgcg gcagcagcgg
      481 gctgggcgcg ggctccggac gggaggtcca aatcgcgatg ggcgcggaca gaaattcgaa
      541 gtttgcggga aggactccgg caaggcgcat gggacggcca tcgaccagat aggtgcggcc
      601 cttgaggttt ggatcggcac gcaaggcgcc acgccagaaa tcgcggctga cgaaaaattc
      661 gtcgtcagca agacggcttg tgggagcggc aacgcccagc acttcaaaaa aagacggatc
      721 gacattcgcg aggcggatgg agcgattgcc gcgcagcgtg acgatcgtac cgttatgcca
      781 ggagtaggcg gccaggccgg aaagtgcctg tgatttgcga cgaaacagtt ccgtttccac
      841 ttcgcggaag ccgaggcgca tgccgaacac gggagggccc tgggccagca cgaccaggcg
      901 accgggatcg gtgtagggaa gccgcgcggc gagctcacgc gtggcggaga atccgccact
      961 ggcgacggca agaatcgcga tggccgcgaa tccggcgaaa agcggcagct ggggatgacc
     1021 caggaaattc gacacctgct cgctgccgga atcggtttcg aagcgagcgg cccaggcggc
     1081 gcgcgcggcg tctaccgtgt gattcagaag ggcgcggcgg gcgtccggat cattcgcctc
     1141 ggcggctttc agcatccagt gccaaaaatt tcccaaccat tcttcgcgcc actgggcgcg
     1201 gagttcctcg gggacaagcc gtgcagcgat tacaaggacg acacgggaga gcgagcgccc
     1261 gagggcaccc gggaatcgcg atgatgagac gatccaatac gcaagccgca tgcgtaaccg
     1321 cctttgattg gagggtagca cgccgcgcgc ccggccacac gcggctagac ggctttcgtg
     1381 gcgccgccgt ccatatcgat cagcgcgccg tggaagaagc ggccttcggg tcctacgatg
     1441 aacgccacca ggctcgcgat gtcttcgggc tcgccaattt gcagggtctt ggcgtcggcg
     1501 gtgaggcgct cttcggcctg gctgagcgcg acgccggtgt ctttcaccac cgcgtcgagg
     1561 cggtgcttca ggcggtccgt gcgaacgtag ccgggcagga tgccgttgac gcgaatttta
     1621 tcgcgcacgc cggtgtcggc cagagatttc gtaaggcaat tcatggcggc gtttaccgat
     1681 ccgccgatgg cgaacatggg tccgggtgtg cgtccgccga tgccggaaat attaacgacg
     1741 gagccgccag aggctttcag atgcggccac gcggcgcgcg taacgcgcac ggcggcaaaa
     1801 aacttgagcg cgaagccatc ggcccaatcg gcatcggtga gctccgtgaa atccccgagc
     1861 ttggtggctc cggcgttgtt cacgacgatg tcgatgcggc cgaatttggc gacggcgaaa
     1921 tcgacaagct gcgcgcaggc ctcgggcatg cggaggtcga gggcgatggt ttccgcgcgg
     1981 ccaccagcct cgcgaatttc atcggcggct tcggacaaaa cggacgcgtc gcgcgcggcg
     2041 aggacaacgt tggctccttc gcgggagagg cgctgggcga tgctccggcc gatgccgcgg
     2101 ttggagccgg taatgacagc gatttttcca gtgagtggct tcatagtaga aaggatttag
     2161 aatttaggaa ttcgttgacc cgagcgtcgt gaagcagtct tccggcgccg ggactttggg
     2221 aagatcgccc aagccttcga gctgtgagat caacgtgagc cacctctcgc gcgtcatgca
     2281 gccgaggccg ttcttgcgcg tctcgtccgt ttcaatcagc ggcttttgcg cttgtgccgt
     2341 ttccgcgaag cccgcgagcg acatcgaagg attcaactga ttcatgcgct ggttggtcgc
     2401 ctgaggatcg tcaaggtagg cgcgccagcc ttcgcgaacg gcagcgacca tcgctttcgc
     2461 ggtttccgga ttcctgcgca gatagtcgcc gctggtggcc atcacggtgt tgtaggggtc
     2521 gaaaccagcg gcggaaatgg cgaacacttg cgcgtcggaa ccctggcgct tcgcggtcaa
     2581 atactccgag gtcatgtaaa cctgctggac gaacttcggg tcgcgaagga acgcgctgat
     2641 atcgccgccg ggcgagggaa ccacctggac tttgtcgaaa ccgtactgcc gttccagaag
     2701 ccgtgcgaaa ggcaggccct tttgaatggc gagcgtgccg ccctttttga agatgtcgcc
     2761 gatatcggcg aacttgcgcg acgcatgaac catgagcgcg gcggggctgt gctgaaacgc
     2821 ggcgtagagt gcgaccacat cgttgccccg agcgcgcgcg acgacaatct catcggcttc
     2881 gacgatgccg aactccgccg atccggcgcc aatcatttgc acgctgggtg ttccggcacc
     2941 gccgggaaga atgtccacat tgaggccgtg ctgcatgtaa ggcgcggcat agaatccgcc
     3001 gaattccggc tcgggcttcc agttcaaggc aagcctgatt tttcccggcg tcttattaca
     3061 tgccgcggaa catgctgcga gcaaggcggg cgggatcagc gaaagaaaat gtcggcgatg
     3121 cacgctctca ttgtctccga tgttcgagag tgggatgtct gggagtgggg cgtgattatt
     3181 gttccgacgc atgccagcgg cgcagggtga aataactcac cagattgatc aggccgaaga
     3241 gcgccagacc gagcacggac gcgaggagaa ccgccgcgaa cactttatcc acgcgttgct
     3301 gcgtgcgcgc gacatccacc acggagccga ggccctggcc tccaatgaat tcgccgacaa
     3361 tcgctccgat cacggcgagt cccgcggcga cacgcaggcc ggtgagaatc tgcggcagcg
     3421 cggaaggaaa gcgcaatttg acgagcgtag tccacgtgga tgcgccgtac aggcggaaga
     3481 ggtcgcgcaa gggaggttcg gtggagagga ttccggtgag cgtgttggcg atgaccggaa
     3541 agatcgacac gacgaaggcc gaggctacca ccgtgcgcat gccatagccg aaccagatga
     3601 ccaggagcgg cgcgatagca atgatcggca cggtttggaa gaagacggcg taaggataga
     3661 aggcgcgctg gatgggtcgc gacgaagaaa gaaaaacggc gaccgcgatg ccgccgatgg
     3721 tggaaagcgc gaagccgagg agggcgctgg cggaagtgga ggcgactgcg gtgaacagct
     3781 cgtcacggct atcgatcatc gctctaagga cctggctggg ggcaggcacc aggtaggaat
     3841 gcacccagcc ctggcgcacg gcaagctcga cggaggccgt gaggacgacg aaggggatca
     3901 gcgcgggaat gatggatttc aggcgcgaca cgcagactcc gt
//
LOCUS       DASZMC010000189         7733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4743757,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000189 DASZMC010000000
VERSION     DASZMC010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4743757"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VG274_03730"
     CDS             complement(<1..368)
                     /locus_tag="VG274_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05791.1"
                     /translation="MNAVRSALDEIARAVDALRTGAFVRLVDGDGAHTTALAVETADE
                     ETIRRLETTGDFLLVLTPARAQTLKIRLYTRDAIAIRLQPPLNIARLRAIADPTFDLA
                     EPLKGPFETVRENLPGTFPAA"
     gene            476..1159
                     /locus_tag="VG274_03735"
     CDS             476..1159
                     /locus_tag="VG274_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWE05792.1"
                     /translation="MPSVKRVLLVDDDAMLRSSLAEQLEQEDGFSPVEAGNCAEAREL
                     AQKGLYEFMILDVGLPDGDGRALCRQFRSEGITCPIILLTAKDSDSDIVEGLKSGAND
                     HISKPFRFAVLAARIHAHLRSHEQSEEAVYRIGHYTFRPSAKLLLDPKGKKIRLTEKE
                     TNILKHLHRSGATVARETLLHEVWGYNPAVTTHTLETHIYRLRQKIESNPGEAQILVT
                     ESGGYRLEA"
     gene            1285..2214
                     /locus_tag="VG274_03740"
     CDS             1285..2214
                     /locus_tag="VG274_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05793.1"
                     /translation="MSSRGKSLLGGAAFAALLIWPACAANGDWVVWPTKSFDANAVKL
                     EGIVGSLTVNVRSRGPLTVDVAGSKPRVNGVRVTKGGNRVVIDGSGFSENNSVWDWKN
                     WFNFSADFSQRADNLTIKVTVPKGASVDVNDLVGDATIGDTQGALHFEAASTKARIGR
                     VARADVSLGGAGKLDIAQVSGPLHLEVGGSGKVTVGPTQSVHADIAGSGDAQFGAIAG
                     GLSLDIAGAGDVSAASVNGPTHIDIAGSGSVKIADGTANPLHVDIMGAGNFIFGGVAV
                     DPHIEAVGSGNVKLKSYRGHLDTEGMADVKIGE"
     gene            2635..4542
                     /locus_tag="VG274_03745"
     CDS             2635..4542
                     /locus_tag="VG274_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWE05794.1"
                     /translation="MTRPSVASCASRPRSTWPYALALLFAWILVIGAVVCFRWFSQLP
                     DTRDLLSRAPSADITLMDARGRLIARRGLIFGRIVDSRSLPSYVPNAFIAIEDKRFRA
                     HIGLDPIGLVRAAAENFIAGRVVQGGSTLTQQLAKNLFLESRRSMDRKFQEALLALYL
                     ESRFNKDQILTLYLNRVYFGAGVYGIEAAAERFFGRSATALSLPEAAMLAGSVKAPTR
                     FNPLADADASLARSVMVLRAMEDAGFISHATRLAAAAVRPRVQRASSTPDSGYFVDWV
                     VAQIPGFAGEANEPLIVETTFDVDAQRGAERAVETGLAREGAKLHVGEAALVAMTPDG
                     AVRAMVGGRSYVQSPFNRATDASRQPGSAFKPFVYLAAFEHGRKADDLMNDGPLDIHG
                     WRPGNYEGRYEGAITLTRAFAKSSNAVAAQLTEETGAAAVSAVAHRLGIVSPLAAVPA
                     LALGVSSVTPLELTGAYATFAGGGVGVAPYGIIRIRTKSGRLLYLHKPARIGRVMSPE
                     NAAAVTGLMVATVTSGTGRAARLDDRSDAGKTGTTENFRDAWFVGFTADLVCGVWTGN
                     DNNAPTRHATGGALPARIFRSFVEVAEQGRPARPLLSLPATLAQTAEQIAAPKQPDAF
                     QRLLDSLFNGT"
     gene            complement(4546..5298)
                     /locus_tag="VG274_03750"
     CDS             complement(4546..5298)
                     /locus_tag="VG274_03750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWE05795.1"
                     /translation="MPNSQTPARTRRHPRRELLRIDGRPVEITVRLNPRARRLIVKVH
                     PSTGEVSVVAPSRRALDHALDFARSESTWIAGRLAHVPEPVDLEPGARIPFRGEDHAI
                     VRAARGPAPVWIERGARTIRICGQNEHAPRRLLDFLKREARRVLDARALEFAARVGVK
                     PRRITVRDTASRWGSCSTTRALSFSWRLILAPPFVLDYVVAHEVAHLRHMNHGSRFWD
                     LVRELVGNVEKPQAWLSQNGPLLHRYAPRVTP"
     gene            5361..5783
                     /locus_tag="VG274_03755"
     CDS             5361..5783
                     /locus_tag="VG274_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE05796.1"
                     /translation="MTARPRFHLAFPVRDLAEARAFYGGLLGCREGRSADHWVDFDFF
                     GHQIVAHLSPAAAAHKSHNCVDGDAVPVRHFGVILDLPAWHDLAGKLKKKEVVFLIEP
                     TVRFQNQPGEQATMFFEDPSGNALEFKAFADDVMVFAK"
     gene            5780..6268
                     /locus_tag="VG274_03760"
     CDS             5780..6268
                     /locus_tag="VG274_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HWE05797.1"
                     /translation="MSRAQLIAVALGTASVAFLLAALGFQYLGRLPPCELCLWQRWPH
                     VGAAVVGLGGSGLIRIGLLERGAARGVAGLAAFLIAISGAVAVYHAGVEWHWWPGPQT
                     CTGDAFRYTGGRLDLNAPMVLCDIAAWRLFGLSLAGYNALFSLGIAGVAGVLLVRSGR
                     KG"
     gene            6265..6828
                     /locus_tag="VG274_03765"
     CDS             6265..6828
                     /locus_tag="VG274_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HWE05798.1"
                     /translation="MTAKRGRRPAAPGVLSIQDIAEMIRVDHAGEYGAKHIYAGQLAV
                     LGSGPRDRKGAAAVRRMAAQEQRHLERFEELIHERKVRPTILSPVWQVTGFALGAATA
                     FLGLKAAMACTASVEEVIDEHYARQIERLDGDPELKAAVTKFREDELAHRGEALAHGA
                     ADTAGYRLLSEAIKAGCRLAIRLSEKI"
     gene            complement(6999..7556)
                     /locus_tag="VG274_03770"
     CDS             complement(6999..7556)
                     /locus_tag="VG274_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWE05799.1"
                     /translation="MNAHVSPSGWPALVLNADFRPLSYYPLSLWSWQDAIKAVFLERV
                     NILAEYERTVRSPSFEMKLPSVVSLKTYVRPARHPAFTRFNVFLRDRFECQYCGSGED
                     LTFDHVVPRSRGGKTTWDNVVTACAHCNLLKGGRLAHHIGMHPRHHPRKPTINELRSN
                     GRAFPPNYLHESWADYLYWDSVLEP"
BASE COUNT         1357 a         2392 c         2557 g         1427 t
ORIGIN      
        1 gcggcgggaa aagttccggg caggttttcg cggacggttt cgaatggtcc cttcaacggc
       61 tccgcaagat cgaaagtggg atcggcgatc gcccgcaacc gcgcgatatt gagtggcggc
      121 tgcaagcgaa tggcgatggc gtcgcgcgtg tagagccgga ttttgagcgt ctgggcgcgc
      181 gcgggcgtca acaccagcag aaaatcgccg gttgtctcaa ggcgccggat tgtttcttcg
      241 tccgccgttt cgacggcaag cgcagtggta tgcgcgccat cgccgtccac aagacgcacg
      301 aaggccccgg tgcgcaatgc gtccaccgca cgggcgattt cgtcgagcgc cgagcgaacc
      361 gcgttcatgc gtttcgactc aagggttgga gccggccggg acaacatgcg ccccacatac
      421 accgggcagg caaatcgggg tagagtcgga tcaaagagcg agcaaggagt ggcaaatgcc
      481 gagtgtgaag cgggttctgc tggtcgacga cgacgcgatg ttgcgctcgt cgctcgccga
      541 gcagctggaa caggaggacg gcttcagtcc cgtcgaagcg ggcaactgcg ccgaggcgcg
      601 cgagcttgcg cagaaaggac tgtatgaatt catgatcctg gacgtgggcc tgccggatgg
      661 agacggacgc gcgctttgcc gccagttccg cagcgaagga atcacctgtc cgatcattct
      721 gctgaccgcg aaggactcgg acagcgacat cgtcgaaggt ctcaaatccg gcgccaacga
      781 ccacatctcc aaaccgttcc gctttgcggt gcttgcggcc cgcatccatg cccatctgcg
      841 aagccacgag cagagcgaag aggcggtcta ccgcatcggg cactacacgt tccggccgag
      901 cgcgaagctg ctcttggatc cgaaagggaa aaagatcagg cttacggaaa aggagaccaa
      961 catcctcaag cacctgcacc ggtccggcgc gacggttgcg cgcgagacgc ttctgcacga
     1021 agtctggggc tacaatccgg cggtcaccac ccacacgctc gagacccaca tctatcggct
     1081 gcgccagaag atcgagagca atccgggcga agcgcagatc ctggtcactg aaagcggcgg
     1141 ctacaggctg gaggcctgag acggaggcat ccgcgctagt ttgggcgtat gctgaccggg
     1201 catggcggcg ctgacttttt tgcgttctat acgcacgagt ttcgccggct tatctcggtt
     1261 ccggcaccaa ggaggtcagg gctcatgagc agcaggggca agtcgttatt gggcggagcc
     1321 gcgtttgctg cgctcctcat ctggcccgct tgcgccgcga atggagactg ggtcgtgtgg
     1381 ccgacaaaga gtttcgatgc caatgcggtg aaactcgaag gcatcgtcgg ctcgctgacg
     1441 gtgaatgtcc gcagcagagg ccccttgacg gtggacgttg cgggctcgaa gccacgcgtc
     1501 aacggggtca gggtgaccaa aggtggcaac agggtggtga tcgatggttc cggcttcagc
     1561 gaaaacaact cggtctggga ctggaagaac tggttcaatt tctccgcgga tttttcgcag
     1621 cgcgccgaca atctgacgat caaggtgaca gttcccaaag gcgccagcgt cgacgtgaac
     1681 gatctcgtcg gcgatgcaac gatcggcgac acgcaaggcg cgctccattt cgaagcggct
     1741 tcgaccaagg ccagaattgg ccgcgtcgcc cgcgccgatg tttcgctcgg cggggcaggc
     1801 aagctcgata tcgcccaggt gagcggaccc cttcatcttg aagtcggcgg atcggggaag
     1861 gtcacggtcg gaccgacgca gagcgtgcat gccgacatcg ccggatcggg cgatgcccag
     1921 ttcggcgcga tcgccggcgg cctgtcgctc gacatcgcgg gcgcgggcga cgtctcggcg
     1981 gcgagcgtca acggccccac ccatatcgac atcgcgggtt ccggctccgt gaagatcgcc
     2041 gacgggaccg ccaatccgct gcatgtcgat atcatgggtg ccggaaattt catcttcggc
     2101 ggcgtcgccg tcgatccgca catcgaggct gtcggctcgg gcaacgtgaa gctcaaatcc
     2161 tatcgcggcc atctcgacac cgagggcatg gccgacgtga agatcggcga gtgacgcgag
     2221 cgccctggcg ccgcgattcc aatccctcgc aagcaaaaaa ttgctgtggc ccgtttgcag
     2281 gaaagtgcgc tacgataacg gagctcgaga tgcccctcac ccgttttgga gcggttgcga
     2341 gcactcgaat gtttcccctc tcccacgagg ggagatgggg cttttccgct gattttctgc
     2401 gcttatgcag cactctgaat tcccctctcc ctctgtggga gagggaagca gatacgtgcg
     2461 tgcattcgtt ttgctcgggc gcgggtgagg ggtccctcgt actgcgttag ctggccaaat
     2521 atgtttaaca gccgttaacg atcatccggc ttaagtgctt cggctcagag ccggaaaacc
     2581 atggcgccga agccttcgtc ttcgcctcgc cttcgaaaac gtcccgatgc tgcaatgacg
     2641 cggccctcgg tcgcttcgtg cgcttcgcgt ccgcgctcca cctggcccta cgcactcgct
     2701 ctcctgttcg catggattct cgtcatcggc gcggttgttt gctttcgctg gttctcgcaa
     2761 cttcctgaca cgcgggatct gctgtcacgc gcgccgtccg ccgacatcac gctgatggat
     2821 gcgcgcggcc gccttatcgc gcgccgcggt ttgatcttcg ggcgcattgt cgattcgcga
     2881 agtcttccct cttacgtgcc caacgcgttc attgcgatcg aggacaagcg cttccgcgcc
     2941 catatcggtc tcgacccgat cgggctcgtc agggccgcag ccgagaattt catcgcgggc
     3001 cgcgtggtgc agggcggctc tacgctcaca cagcaactcg cgaaaaacct gttcctcgaa
     3061 tcccggcgaa gcatggaccg caaattccag gaggcgctcc tcgctctcta tctggaatcg
     3121 cgattcaaca aggatcagat tctcacgctc tatctcaatc gggtctattt cggagctggc
     3181 gtctatggca tcgaggcagc cgcagagcgc ttcttcggca ggtccgccac ggcgctgtcc
     3241 ctgcccgaag ccgcgatgct cgccggaagc gtgaaggcgc ccacgcgctt caatccgctc
     3301 gccgacgcgg atgcttcgct ggcccgttcg gtgatggtgc tgcgcgcgat ggaagacgcg
     3361 ggcttcatct ctcatgcgac ccgtctggcg gccgccgccg ttcggccccg cgtccagcgc
     3421 gcgtcctcca cgccggattc gggttatttc gtcgattggg tcgtagcgca gattcccggc
     3481 tttgccggcg aggcgaacga gcccctgatc gtcgagacga cgttcgacgt ggatgcgcag
     3541 cgcggggccg agcgcgcggt ggaaaccggc ctcgcgcgcg aaggtgccaa attgcacgtg
     3601 ggcgaggccg ctctggtggc gatgacgccg gatggagcgg tacgcgccat ggtcggcgga
     3661 cgctcctacg tacagagccc tttcaatcgt gcgaccgatg cctcacgcca gcccggttcc
     3721 gcattcaagc cgttcgtcta tctcgcggcg ttcgagcacg gccgcaaagc cgacgatctg
     3781 atgaacgatg gcccgctgga cattcacggc tggcggccag gcaactacga gggccgctac
     3841 gagggcgcga ttacgctgac gcgcgccttc gcgaaatcgt cgaacgccgt cgccgcacaa
     3901 ttgaccgagg agaccggcgc cgccgccgtc tctgccgtcg cccatcgtct gggcatcgtc
     3961 tctccgctcg cggcggttcc ggctcttgcg ctgggcgtct cgagcgtcac gccgctggag
     4021 ttgaccggag cctacgcaac tttcgcaggc ggcggcgtcg gtgtcgcacc gtacggaatc
     4081 attcgcatac gaaccaaatc cggacggctg ctttacttgc acaagcctgc gaggatcggg
     4141 cgcgtgatgt cgccggaaaa cgccgccgcc gtaaccgggc tgatggtcgc gacggtcacc
     4201 agcggcaccg gcagggcggc gaggctcgac gatcggtccg atgcgggcaa gacaggtacg
     4261 acggagaatt ttcgcgacgc ctggttcgtt ggattcacgg cggatcttgt ctgtggcgtc
     4321 tggactggaa acgataacaa cgcgcccacg aggcacgcga cgggtggcgc cttgccggcg
     4381 cgaatcttcc gttctttcgt ggaagttgca gagcagggca ggcccgctcg gccactgctt
     4441 tcccttcccg cgaccctcgc gcagacagcc gaacaaatcg cggcgcccaa gcagcctgac
     4501 gcctttcagc gccttctcga cagcctgttc aatggaacct gaatctcagg gcgttacgcg
     4561 cggagcgtag cgatgcagga gcggtccgtt ctggctgagc cacgcttggg gtttctcgac
     4621 gtttcccacc agttcgcgaa ccagatccca gaatcgcgaa ccgtgattca tgtgacgcag
     4681 gtgcgcgact tcgtgcgcca ccacgtagtc gagcacaaaa ggcggtgcga gaatgaggcg
     4741 ccacgaaaac gaaagcgcgc gcgttgtcga gcatgaaccc cagcggctcg ccgtatcccg
     4801 gacggtgatg cgcctgggtt tgacgcccac gcgggcagca aactcgagcg cgcgggcgtc
     4861 aagcaccctg cgcgcttcgc gcttcaggaa atcgagaagg cgtcgcggcg catgttcgtt
     4921 ctggccgcag atgcgaatgg tgcgcgcgcc gcgctcgatc cataccggcg cggggcccct
     4981 ggctgcacgc acgatcgcat gatcttcgcc gcgaaaggga atgcgcgccc ccggctccag
     5041 atcgacgggc tcgggaacat gtgcgaggcg tcccgcgatc caggtcgatt ccgagcgcgc
     5101 aaaatccagc gcatggtcga gcgcgcgccg cgatggtgcg acgacgctga cctcgcccgt
     5161 cgagggatgg accttgacga tcagacggcg cgcacgggga ttgaggcgca cggtgatttc
     5221 gacgggacgc ccatcgatgc gcagcagctc gcggcgtggg tggcggcgcg tgcgggcggg
     5281 cgtctgggaa ttcggcatgg gacttccttt taccggtctg acttgcttct ctcgcacatc
     5341 acccaaagag gccatttggc atgacagcgc ggccccgctt ccatctcgcc ttccccgtgc
     5401 gcgacctcgc cgaagcacgc gccttctacg gtgggctcct cggttgccgg gagggccgat
     5461 ccgcggacca ctgggtcgac ttcgactttt tcgggcacca aatcgtcgcg catttaagcc
     5521 ccgccgcggc cgctcacaag tcgcataatt gtgtggatgg cgatgcagtt cccgtgcgtc
     5581 actttggcgt catactggac ctcccggcgt ggcacgattt ggctggaaaa cttaagaaaa
     5641 aggaggtggt gttcctgatc gagccgacgg tgcgctttca aaaccagcca ggcgagcagg
     5701 cgacgatgtt tttcgaggat ccgagcggca atgccctgga gttcaaggca ttcgccgacg
     5761 acgtcatggt gttcgcgaag tgagccgcgc gcagcttatt gcggtcgcgc tgggaacggc
     5821 ctcggtagcc tttctcttgg ccgcgctcgg cttccagtat ctgggccgcc tgccgccctg
     5881 cgaattgtgt ctgtggcagc gctggccgca tgtgggcgcg gccgtggtcg gtcttggcgg
     5941 gtcggggctg atccgaatcg ggcttctgga gcggggcgcc gcccggggcg ttgccggact
     6001 tgcagcattc ctgatcgcga tttccggcgc ggtcgccgtc taccacgccg gggttgaatg
     6061 gcattggtgg ccgggtcctc agacctgcac gggagacgcg ttccgctaca cggggggccg
     6121 gctcgacctg aacgcgccga tggttctctg cgacatcgcg gcgtggcggc tcttcggcct
     6181 ctccctcgcg ggttacaatg cgctgttttc gctcggaatt gccggcgtgg ccggtgttct
     6241 gcttgtgcgc tcggggagga aggggtgact gcgaagcgcg gccggcgccc ggccgcgccc
     6301 ggcgtgcttt ccatccagga tatcgctgag atgatccgtg tggatcatgc cggcgagtac
     6361 ggcgccaagc acatctatgc ggggcagctc gccgttctgg gctcggggcc acgcgaccgc
     6421 aagggagctg ccgcagttcg gcggatggca gcgcaggagc agcggcatct cgagcggttc
     6481 gaagagctta tccatgagag aaaggtgcgg ccgacgatac tctcgcccgt atggcaggtg
     6541 accgggttcg cgctcggcgc tgccacggcc tttctcggcc tgaaggcggc gatggcttgc
     6601 acggcgtccg tggaggaagt catcgacgag cattatgcga ggcagatcga gcgtctcgac
     6661 ggcgatcccg agctgaaggc agccgtgacg aagtttcggg aagacgagct ggcgcaccgc
     6721 ggcgaggccc tggctcatgg cgccgccgac acggccggct accggctgct cagcgaagcc
     6781 atcaaggccg gttgccggct cgcaatcagg ctttcggaga agatatgaac gacggaccgc
     6841 gcaggggtga cgccgaccgc cgcacgtgtt ggccacgacc gcttcacgcc gccgggcttc
     6901 ggcttccgag acgcgcgacc tcacgggact ttttgccgca gcgttcttgg ccgatccggt
     6961 gatgggctgg acgccgccgc tgccggccct ccgggccgtc aaggctccag aacgctgtcc
     7021 cagtagagat agtccgccca ggattcgtgc agatagttcg gcggaaacgc gcgcccgttg
     7081 ctgcgcagct cgttgatcgt tggcttgcgg ggatggtgcc ggggatgcat gccgatgtgg
     7141 tgggccagcc ggccgccctt cagaagattg cagtgtgcgc aagcggtgac gacgttgtcc
     7201 caggtcgtct tgccgccgcg cgagcgggga acgacgtgat cgaaagtgag gtcttcgccg
     7261 gacccgcaat actggcactc gaagcgatcg cgcagaaata cgttgaaccg tgtgaatgcc
     7321 ggatggcggg cgggcctcac ataagtcttc agcgacacta ccgagggcag cttcatctcg
     7381 aagctgggag agcgcacagt gcgctcatat tcggcgagaa tattcacgcg ctcgagaaag
     7441 acggccttga tggcgtcctg ccatgaccac agcgacagtg ggtagtaact cagcggccga
     7501 aagtccgcgt tcagaaccag cgcgggccag ccagagggcg atacatgagc gttcaagctg
     7561 acgctcccac tcgagtcaat gccgcagtgc ggcgatggcc gacagtttat aggggggcgt
     7621 gacaggattg tgaagcctgt tccaggggtt atgcgacacc ccgatttatt ggcttttact
     7681 ggtagggttt gcggtggcaa tgcgctcgga ctacggcgaa ttgccgacga ggc
//
LOCUS       DASZMC010000190         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3638993,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000190 DASZMC010000000
VERSION     DASZMC010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638993"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1528
                     /locus_tag="VG274_03775"
     CDS             <1..>1528
                     /locus_tag="VG274_03775"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE05800.1"
                     /translation="AAAVAERQFLAAVKHSNIVGIYNFVQNGGEGFIVMEYVGGKTLK
                     QIRQERGPLPPAEAVAYIHRILSAFAYLHRTGLVYCDFKPDNVMVEKDDVKLIDMGGV
                     RRIDDLKGDIYGTVGYSAPEAAEGPTAASDLFTVGRTLAVLLTEIHGFSKEHLYTLPN
                     VQEAPVFANQESLYRVLLKATAQHSDDRFESADDMAEQLLGVLREIVSIDTGVPRPSP
                     SVFFGGDLLSFHTADGLEQIYADHRYLPVPSFDMTDPGAQAAAAGDALGDPFRRANAL
                     RVASAQFPKSREVPLRLAAALIDGGEFADAVAQLKRLAEQDAWDWRVLWFQARMKMTE
                     RNFAEARKLFDQVYFDLPGELAPKLALGVAAELSSEWSLAISMYDLVSRTDPGLVSAV
                     FGLARCHHAKGERQAAVTALHRIPPSSAMYTCSRVEAARLLIERNHTTPTAGELAEAS
                     AVTQSLALEGRSKHTLDSHIYRAALDLITSGAVKEDRAVAVLGGPLQEPALRRGLEIS
                     L"
BASE COUNT          277 a          556 c          422 g          273 t
ORIGIN      
        1 gcggccgccg tcgccgagcg ccagtttctg gctgcggtca aacactccaa catcgtcggc
       61 atctacaact tcgtacagaa cggcggcgaa ggcttcatcg taatggagta tgtgggcgga
      121 aaaacgctca aacagattcg tcaggaacgg ggtccgctgc ctccggccga agccgtcgcc
      181 tacatccacc gcatcctctc cgccttcgcg tatctccacc gcaccggcct ggtctattgc
      241 gacttcaagc ccgacaacgt catggtcgaa aaggacgacg tcaagctcat cgacatgggg
      301 ggcgtccgcc gcatcgacga tctcaaaggc gacatctacg gcaccgttgg ctacagtgcg
      361 cccgaagccg cggaaggtcc cacggccgcg tccgatctct tcaccgttgg ccgcactctg
      421 gcagtgctgc tcactgaaat tcacggcttc tcgaaagagc atctctatac tctgcctaac
      481 gtgcaggaag cgcccgtctt cgccaatcag gaatcgctct atcgcgtcct cttaaaggcc
      541 accgcgcaac actccgacga tcgcttcgaa agcgccgacg atatggcgga gcagctcctc
      601 ggcgtcctgc gcgaaatcgt ctccatcgac accggcgtcc cacgtccctc ccccagcgtc
      661 ttcttcggcg gcgacctgct tagcttccac accgccgatg gcctcgaaca gatctacgcc
      721 gatcatcgct acctgcccgt gccctcgttc gacatgacgg atccgggggc ccaagcggcc
      781 gccgcgggcg acgcgctggg cgatccgttc cgccgcgcca acgcgctccg cgtagcctcc
      841 gcgcaattcc ccaagtcccg cgaagtcccg cttcgtctgg ccgcggctct catcgatggg
      901 ggcgagttcg ccgatgccgt cgcgcagctc aaacgcctcg ccgaacaaga tgcatgggac
      961 tggcgcgtcc tctggtttca agcccgtatg aagatgaccg agcgcaattt cgcggaggcc
     1021 cgcaaactct tcgaccaggt ctatttcgat ctgcctggcg aactcgcgcc caaactcgcg
     1081 ctcggcgtag ccgcggagct ttcgagcgaa tggagtctcg ccatcagtat gtacgatctg
     1141 gtttcgcgca ccgatcccgg cttggtctcc gcggtcttcg gcctcgcgcg ctgccatcat
     1201 gcaaagggcg agcgccaggc cgcggtcacc gcgcttcatc gcatcccgcc gtcgtcggcc
     1261 atgtacacgt gctcgcgcgt cgaagccgcg cgcctgctca tcgaacgcaa tcacaccacg
     1321 cccaccgcgg gcgaactcgc cgaggcctcc gccgtcacac aatcgctggc gctcgaaggc
     1381 cgcagcaaac acacgctcga tagccacatc taccgcgctg cgctcgatct gatcacctcc
     1441 ggtgcagtaa aagaagatcg cgccgtcgcc gttctgggcg gccctctgca agagccagcg
     1501 ctgcgccgcg gcctcgagat ctcgctac
//
LOCUS       DASZMC010000191         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1195286,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000191 DASZMC010000000
VERSION     DASZMC010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195286"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /gene="bioD"
                     /locus_tag="VG274_03780"
     CDS             complement(<1..453)
                     /gene="bioD"
                     /locus_tag="VG274_03780"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HWE05801.1"
                     /translation="MSAYFVTATGTDIGKTYVTAGLIRHLRAGNAPVFALKPVVSGFD
                     MAEAQASDPGILLRALGESVTPQAIARTSPWRFSAPLSPDMAAAREGRTIPFHELVAF
                     CRDEIASTTGTLFIEGVGGVMVPLDAENTVLDWIAALHAPVILVAGTYL"
     gene            complement(450..1610)
                     /locus_tag="VG274_03785"
     CDS             complement(450..1610)
                     /locus_tag="VG274_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWE05802.1"
                     /translation="MSTLDAFAIAKLDQLTGQHLKREPHETWREDGIWVHRAGRRLLS
                     VSCNDYLNLTHHPDVKAAAVAAIQTYGAGAGASRLVTGNHPLLGNLEQRLALLKGTEA
                     ACVFGSGYLANAGIVPVVIAPDDLLLLDELSHACLFAGAQLSRARVEVFRHNDMSHLR
                     ALLSSHRANHPHALILTDGVFSMDGDLAPLQELSCVAGKHDSWLMTDDAHGFGVLGQG
                     RGSAFANGTKPHIDLQMGTLSKAIGSYGGYVCASQPVIDLIKTRARTLIYSTGLPPAS
                     AAAASAALDIIEADPALCARPLAKARAFTRTTGLPLAESPIVPIRLGEPELALEAQAI
                     LEREGFLAIAIRPPTVPAGTARLRLAFSAGHPDAEIERLAAIVRDRVLPMTA"
     gene            1691..2671
                     /gene="bioB"
                     /locus_tag="VG274_03790"
     CDS             1691..2671
                     /gene="bioB"
                     /locus_tag="VG274_03790"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWE05803.1"
                     /translation="MNAPLSSSALEHHPRHDWTREEIEALFTLPFPDLLYRAQTIHRA
                     NFDPRAIQLSTLLSIKTGGCPEDCGYCSQSAFHETGLKASKLMDADSVAETARRAKAS
                     GATRFCMGAAWRSPKEKDLDAVCNMVRDVRSLGLETCATLGMLTPAQALQLKEAGLDY
                     YNHNLDTSPGYYGKIITTRTWQDRLDTLAAVREAGIYVCCGGIVGMSESDSDRIGLIH
                     ALATLPAHPESVPINMLMRVKGTKLGDSEPLDPIEFVRMIAVARITMPKSVVRLSAGR
                     EYMSDETQALCFLAGANSIFVGEKLLTTPNPDQDRDRSLFGKLGLIPMPA"
     gene            2677..>2977
                     /locus_tag="VG274_03795"
     CDS             2677..>2977
                     /locus_tag="VG274_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWE05804.1"
                     /translation="MSRAQPAWYAKGLSHIWLPYTQLKTAPPPLAVARTQGSRIILAD
                     GRELVDGIASWWTVCHGYNHPHIREAVQRQLERLPHVMLGGLVHEQALSLAARLCA"
BASE COUNT          571 a          955 c          910 g          541 t
ORIGIN      
        1 gagataagtt ccggcaacca ggatcaccgg cgcatgcagc gcggcaatcc aatcaagaac
       61 ggtattctca gcatcaagcg gcaccatgac cccgcccacg ccttcgatga acaatgtccc
      121 tgtcgtggag gcaatctcat cgcgacagaa ggcgacgagc tcgtgaaatg ggatggtccg
      181 gccttcccgc gccgcagcca tgtccggcga aagcggcgca gaaaaccgcc agggcgaagt
      241 ccgtgcgatc gcttggggtg tcacagattc gcccagagcg cgcagcagga tgccgggatc
      301 gctcgcttgg gcttccgcca tgtcgaatcc gctcaccacg ggtttgagcg caaaaaccgg
      361 tgcgttgcct gcgcgcaaat gacggatcag acccgcggtg acgtaagtct tgccgatgtc
      421 cgttcccgtc gcggtgacga aatatgcgct catgcagtca tcggtaagac gcggtcgcgg
      481 acgatcgcgg ccagccgttc gatctctgca tccggatgtc cggccgaaaa ggcgagcctc
      541 agccgcgccg tgcccgccgg cacagtcggc gggcgaatgg caatcgcgag aaagccttcg
      601 cgctccagga tcgcctgtgc ttccagcgcc agttccggtt cgcccagccg gatggggaca
      661 atcgggctct ccgcgagcgg cagccccgtc gtccgtgtga acgcgcgtgc tttcgcgagc
      721 ggacgcgcgc aaagcgcagg atcggcttcg atgatgtcga gcgcggccga agcggcggcc
      781 gcgctcgccg gcggcaatcc ggtggaatag ataagcgtgc gcgctcgtgt cttgatcaga
      841 tcgatcaccg gctgcgaggc gcaaacatac ccgccatagg agccgatcgc tttggagagt
      901 gtgcccattt gcaggtcgat gtgcggcttt gttccgtttg cgaatgccga acctcggccc
      961 tgcccgagca cgccaaaccc atgggcatcg tcggtcatca gccagctgtc atgcttgccg
     1021 gcaacacaac tcagctcctg cagcggcgcc aaatcgccat ccattgaaaa caccccatcc
     1081 gtcagaatca gcgcatgcgg atggttggcg cgatgactgc tcagcaacgc acgcagatgg
     1141 ctcatgtcgt tgtgccggaa gacttccacg cgcgctcgcg acagctgcgc gccggcgaac
     1201 aggcacgcat gcgacaattc atcgaggagc aacaggtcat cgggtgcaat cacaacgggg
     1261 acgataccgg cattcgcgag ataccccgat ccgaacacgc atgccgcctc tgttcccttg
     1321 agcagggcga gccgctgctc aaggttcccg agaagcggat gattgccagt gacgaggcgt
     1381 gaagcgcctg cgccggctcc atatgtctgg atcgccgcta cggctgcggc ttttacgtcc
     1441 ggatgatggg tcagattcaa atagtcgttg cacgagacgg aaagcaatcg acgtcccgcc
     1501 cgatgcaccc atattccgtc ctcgcgccac gtttcgtgcg gttcgcgctt gaggtgctgc
     1561 cctgtcagct ggtcgagttt ggcgattgcg aaagcatcga gggtggacat ttctggttgc
     1621 gccgcttgca gctttgtgat ccgccgatat aagggcaccg tcgctctatc cacaacgctt
     1681 ggaattgcgc atgaatgccc ctctgtccag ttctgctttg gagcaccacc cccgtcacga
     1741 ctggacgcgc gaggagatcg aggcgctctt cacgcttcca ttccccgatc tgctctatcg
     1801 cgcgcagacg attcaccgcg cgaacttcga tccgcgcgcg atccagctct ccacgctcct
     1861 ttcgatcaag accggcgggt gtccggagga ttgcggctat tgctcgcaaa gcgcctttca
     1921 cgagacgggc ctcaaggcga gcaagctcat ggacgcagat tctgtcgccg agaccgcgcg
     1981 gcgtgcgaag gcgtcaggcg cgacgcgatt ctgcatgggt gcggcgtggc ggtcgccgaa
     2041 ggagaaggac ctcgatgccg tttgcaacat ggtgcgggat gtcagatcgt tgggcctcga
     2101 aacgtgcgca acgctcggca tgctcacgcc cgcgcaggct ctgcagctga aggaagccgg
     2161 actcgattac tacaaccaca acctcgacac ctcgcccgga tactacggaa aaatcatcac
     2221 cacacgcacc tggcaggacc ggctcgatac gctcgcagcc gtccgcgaag ccggcatcta
     2281 cgtttgttgc ggtggtattg tcggcatgag cgaatcggac tccgatcgca ttggccttat
     2341 ccacgcgctc gcgacgctgc cggcgcaccc cgagagcgtg cccatcaaca tgctgatgcg
     2401 cgtgaaggga acgaagcttg gcgatagcga gccgctcgat cccatcgaat tcgtgcgcat
     2461 gattgctgtc gcgcgcatca cgatgccgaa atccgtggtg cggctttccg caggccgcga
     2521 atatatgagc gacgagaccc aggcgctctg cttccttgcc ggcgcgaatt ccatcttcgt
     2581 cggcgagaag ctgctgacga cgcccaatcc ggatcaagac cgcgacagaa gtttgttcgg
     2641 caagctgggc ctgatcccga tgccagcctg aagccaatgt cgcgcgctca gcctgcgtgg
     2701 tacgccaaag gtctgtccca catctggttg ccctacaccc aactgaagac cgcaccgccg
     2761 ccgcttgcgg tggcgcgcac gcagggcagc cggatcattc tcgccgacgg ccgcgagctg
     2821 gtcgacggca tcgcctcgtg gtggacggtt tgtcatggat acaatcaccc tcacatccgc
     2881 gaggcggtgc aacgccagct ggagcgcctc ccgcatgtga tgctcggcgg acttgtccac
     2941 gaacaggcgt tgagtctggc ggcgcgactc tgcgccc
//
LOCUS       DASZMC010000192         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_368672,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000192 DASZMC010000000
VERSION     DASZMC010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368672"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VG274_03800"
     CDS             complement(<1..596)
                     /locus_tag="VG274_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05805.1"
                     /translation="MIWLIGWGLLVVLFLLVADAALREMHSSSLQSAIFSKQASGLTY
                     TIKSGANGRIRFPGNGPYDTRLGYSRLPGFISKLEAKHFVVTRQAVQSPDMRAVAEAQ
                     GYAIYREKQHAGLMLRDREGSPLYEASYPEHSYDKFESVPPLVVRTLLFIEDRDLLET
                     RYPHYNPAVEWKRFLQASAGWMAGWLSPHLRRGGASTLA"
     gene            complement(949..1599)
                     /locus_tag="VG274_03805"
     CDS             complement(949..1599)
                     /locus_tag="VG274_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE05806.1"
                     /translation="MAISWDSREVGVTAPDDAHPAPTLETRRLCLRVHAVSDFEDCAA
                     MWSDPQVMRYLSGRPFTREESWARLLRYVGHWQLLGFGFWAVREKTTGRFVGEVGLAD
                     LRRDLQPSIDGAPEIGWVLSPWAHGKGFATEAARAVLNWADRCFGPVRTVCIIHPGNS
                     ASLRVACKCAYGEFARTTYKDSPVILLERVPEGQNGCQNPDDAHHELRPDGDRFTN"
     gene            complement(1574..3274)
                     /locus_tag="VG274_03810"
     CDS             complement(1574..3274)
                     /locus_tag="VG274_03810"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWE05807.1"
                     /translation="MNDVTPAGRSKTDWEIAQEATLLPIQDIAGKLGIPPESVEPFGH
                     HKAKIAMDFVERRFAGGPDGKLILVTSINPTPAGEGKTTTTVGLGDALNRKGKRTAIC
                     LREPSLGPCFGMKGGAAGGGYAQVVPMDELNLHFTGDFHAIGVANNLLAALIDNHIYW
                     GNPLDIDERRITWRRCLDMNDRALRSIVSSLGGVSNGFPREDGFDITVASEVMAILCL
                     CTSLQDLERRLSNIIVASTRERAPVRASDLKAQGAMTATLKDAIKPNLVQTLENNPVF
                     VHGGPFANIAHGCNSVLATRLGLKLADYVVTEAGFGADLGAEKFFDIKCRKAGLSPSL
                     AVVVVTVRALKFHGGVPRKDLGAENVTAVVKGLANLARHLENVRKFGVPAVVAVNHFA
                     SDTKPELDAVVEGCRKLGTRAVVCRHWAEGGAGAEALADVVIAAIDEGAANFHTLYPD
                     SMKPLQKIETIAFQIYHAGEVVPSKQASDRLAELERFGFGNLPVCIAKTQYSNSADPD
                     AYGAPTGHVLPLREVRLCAGAEFIVAICGDIMTMPGLPRQPSAESIMVDRHGHIVGLS
                     "
     gene            complement(3267..>3843)
                     /locus_tag="VG274_03815"
     CDS             complement(3267..>3843)
                     /locus_tag="VG274_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05808.1"
                     /translation="TAPDLVGAAAAFAMWSLVPVRTPASTAIVATVLCVMVVLIRLEP
                     FEFRAEAGTFGWLPFRSYLSGSLDVAIVSFLEKFFLYGSLIWIGSGAGLRLGVSTVVV
                     AALLFATSLAEVHLPGRSAEITDAAMAIFIGTAFMAMTASARASGNHRMETADLAQHN
                     SPPFKKSAGRVPTGIAATIAGSETERSIHHE"
BASE COUNT          766 a         1188 c         1177 g          712 t
ORIGIN      
        1 gccagcgtgc tggcgccgcc acgcctcaga tgcgggctaa gccagccggc catccagccg
       61 gcggacgcct ggagaaagcg cttccactcg acggcgggat tgtagtgcgg atagcgtgtt
      121 tcgagcaggt ccctgtcttc gatgaaaagc agcgttcgga cgacgagcgg aggaaccgat
      181 tcaaatttgt cgtacgaatg ctcggggtaa ctcgcctcat acaggggtga gccctcgcgg
      241 tcgcgcaaca tcagacctgc gtgctgtttc tctcgataga ttgcgtagcc ctgcgcctct
      301 gcaaccgcgc gcatatcggg ggattggacc gcctggcgcg taacgacaaa gtgcttcgcc
      361 tcgagtttcg aaatgaagcc gggtagccgc gaataaccga ggcgcgtatc gtatgggccg
      421 ttgcccggga atcgaattcg accgtttgct ccgctcttga tcgtgtaggt caggccgctg
      481 gcctgtttgg agaagatggc cgattggagg gaagagctgt gcatctcgcg aagggcggca
      541 tctgcaacca gcagaaacag cacaaccaga agtccccagc cgataagcca gatcaaggtt
      601 ccaatcgatg gccgcgcggg ccagattgcg tcccgcatgt ggcggcgagt tttgtacgct
      661 gctgccagga tgtgctcagc cactgtgggt tcctctccgt cccactatgt ctaagcattg
      721 ccggctgacc gggttccctc gcaccgcgga ttgtcgggcc agagcggatt cacgaaacgg
      781 caataatggg ctgtggtcgc gatgtgcgaa accgaccggg aattacaatg aaaagatgtg
      841 gtgcgcggtc atctggagaa ttaaagcaaa gcgcgaaaat ccacgcggca acgcatggac
      901 ggttttccgg aataaagacg catcgatccg cattcgattc actgatattc agtttgtgaa
      961 acgatcgcca tctggccgca gctcgtgatg cgcgtcgtcc gggttctggc aaccgttctg
     1021 cccttccggt acgcgctcca acaggatgac cggcgagtcc ttgtaggtcg ttcgcgcgaa
     1081 ttcgccatag gcgcacttgc atgccacacg cagggacgcg gaattcccgg gatggatgat
     1141 gcacaccgtt cttacgggac cgaagcatcg gtctgcccag ttcagcactg cccgcgcagc
     1201 ctcggttgca aatcccttgc cgtgtgccca tggcgaaagc acccagccga tctccggcgc
     1261 gccgtcgatc gacggctgga ggtcgcggcg caaatcggca agaccgactt cgccgacaaa
     1321 acgtccggtt gtcttttcgc gcaccgccca aaagccaaac cccagcaatt gccaatgccc
     1381 gacgtagcga aggagccgcg cccacgactc ttccctcgtg aacggcctgc cgctcagata
     1441 gcgcataacc tgcgggtcgc tccacatcgc cgcgcagtcc tcgaaatcgg acacggcatg
     1501 gacacgcagg cacaagcggc gggtttcgag cgtcggcgca gggtgcgcgt cgtccggcgc
     1561 ggtcacgccg acctcacgag agtcccacga tatggccatg tcggtcgacc atgatcgatt
     1621 ctgcggaggg ctgccggggc aggccaggca tcgtcatgat gtccccacag attgcgacaa
     1681 tgaactccgc gcctgcgcaa agccgcactt cgcgcaatgg cagcacatgt cccgtgggcg
     1741 cgccataggc gtccggatcc gcggaattgg aatactgggt cttggcgatg cagaccggca
     1801 gattcccgaa accgaacctc tcgagttccg ccagacggtc tgaggcctgc ttcgacggca
     1861 ccacctcgcc cgcgtggtag atctgaaacg cgatcgtttc gattttctgc aatggtttca
     1921 tggagtcggg atatagcgtg tggaagtttg cagcgccctc gtcgattgct gcgatcacca
     1981 cgtcggcgag agcctcggcg ccggcgccgc cctccgccca atgtcggcag acgacagccc
     2041 gcgtaccaag cttgcggcag ccttccacga ccgcgtcgag ttccggcttc gtgtcagatg
     2101 caaaatggtt caccgctacc accgccggca cgccaaactt ccggacgttc tcgagatggc
     2161 gcgcaagatt ggcgaggcct ttgacgaccg cggtaacgtt ttccgcgccc aaatcctttc
     2221 ggggaacacc gccatggaat ttcagcgccc ggaccgttac gacgaccacg gcgagcgaag
     2281 gcgacagtcc cgccttgcgg catttgatgt cgaagaattt ctccgctccg agatccgcgc
     2341 cgaacccggc ctcggtcacc acataatccg caagtttcag gccgagcctg gtcgccagga
     2401 cggaattgca gccgtgagcg atgttggcga acggcccgcc atgcacgaag accggattgt
     2461 tctccagcgt ctgcacgaga ttgggcttga tcgcgtcctt gagtgttgcg gtcatcgcgc
     2521 cctgcgcctt caggtcgctc gcccggaccg gggcacgttc ccgtgtcgaa gccacgatga
     2581 tgttcgacag gcgtcgctca agatcctgga gcgaggtgca aagacaaagt atcgccatca
     2641 cctccgaggc gacggtgatg tcgaatccat cctcgcgcgg gaagccgttg gaaacaccgc
     2701 caagcgagga gacgatcgag cgcagggcgc ggtcgttcat atcgagacag cgccgccacg
     2761 tgatccgccg ctcgtcgatg tcgagcggat tgccccaata gatgtgattg tcgatcagcg
     2821 cagcaagcag attgttggcg acgccgatgg catggaaatc tcccgtgaag tgcagattga
     2881 gctcgtccat cgggacaacc tgggcataac cgccgcccgc cgcgccaccc ttcatgccga
     2941 aacagggccc aagactcggt tcgcgcagac aaatcgccgt ccgcttgcct ttgcgattga
     3001 gggcgtcgcc gaggccgaca gtggtggtcg ttttgccctc gccagccggc gtcgggttga
     3061 tcgacgtaac cagaatgagc ttgccgtccg gcccaccggc gaagcggcgc tcgacgaaat
     3121 ccatggcgat cttcgccttg tgatgcccga agggttcgac gctttcgggc ggaatgccga
     3181 gtttgcccgc gatgtcctgg atcgggagca gggtcgcttc ctgagcaatt tcccagtccg
     3241 tttttgagcg gccagcggga gtgacgtcat tcatggtgga tgcttctttc cgtttccgag
     3301 ccggcgatcg tcgccgcgat gcctgttggt acccgcccgg cgcttttttt aaaaggcggc
     3361 gaattatgct gcgccagatc ggcggtctcc atgcgatggt tcccgctcgc tcgcgcggaa
     3421 gccgtcattg ccataaaggc ggtgccgata aagatggcca ttgcggcgtc ggtgatttct
     3481 gcggaccggc caggcaaatg gacttccgcg aggctggttg caaagagcag cgcggccacc
     3541 acgaccgtcg atacgccaag tcgcaatccg gctccgctgc cgatccagat caggctgccg
     3601 taaagaaaga atttctccag aaacgacacg atggcgacgt cgagcgatcc gctcaaataa
     3661 ctgcgaaacg gcagccaccc gaacgtgcca gcctccgcgc ggaactcgaa gggctcgaga
     3721 cgaatcaaaa ccaccatgac gcacaagacg gtggcgacaa tcgcagtcga tgccggggtt
     3781 cgaacaggaa cgagggacca cattgcgaag gcggccgcgg cgccgaccag gtcaggcgcc
     3841 gtc
//
LOCUS       DASZMC010000193         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4747458,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000193 DASZMC010000000
VERSION     DASZMC010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4747458"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VG274_03820"
     CDS             complement(<1..598)
                     /locus_tag="VG274_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWE05809.1"
                     /translation="MNRRAFLETATALPALAQATRAETGIKLGFDTYSLRAFGWKDKQ
                     LIDFAARMKCDTLQISDSADYSSTDAAHLREVRDYAAKAGIQIDAGIGCICPLSKSWK
                     PNQGTPEQVIADGLRVAHTVGATSMRCFVGTMDDRRGSRPIEEYMDAAIKILRRVKSR
                     AQDANVKIAIENHAGDMQAREVRTMIEEAGKDFVASCLD"
     gene            677..>2195
                     /locus_tag="VG274_03825"
     CDS             677..>2195
                     /locus_tag="VG274_03825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWE05810.1"
                     /translation="MAPPTDASAALVVVDPNGRRHRAALASFPFRIGRAADNNLVLRD
                     SRVSRNHAQILFDGCQFVVEDLGSLHGVWVNGERVGVRRPLDGSESIEFGVPDGYRLH
                     FTRSRDELQKLLARPLPSDTSRSGGANLEKLRAVLEVARSLQSSFSVDDVLNTLVDAA
                     LAVTGAERGFLLLFDAAHELQTRSARSRGGRDLPASDLRVPCRLIQQALEGRRDLFYM
                     SFDPAALECAPGNTISVLELRSVACIPLVRVNLRRADTQPLPQSAANLGVLYMDSRGG
                     AVDLAGGNRELLQTLAIEASTVLENARLLEEERVRQRVEEEMDLARRIQQSLLPTALP
                     NTGWFRVCGSSEASHQVGGDYFDVAQKSAGCWSLVVADVAGKGVGSALMASFLQGAFL
                     SASGAEPIPELMARINAFLSERAEYGKYATMFYATLDRAGALTYANAAQCTPYLLRKD
                     AAPKDSKIEPLESTCRPLGLLPEGDFPAEQRHLRAGDRLIVYSDGVTEARNDAGEF"
BASE COUNT          355 a          799 c          630 g          411 t
ORIGIN      
        1 tatcgaggca cgacgccaca aaatctttgc ccgcctcctc gatcatggtc cgcacctcgc
       61 gcgcctgcat gtcgcccgcg tggttctcga tcgcgatctt cacgttcgcg tcctgcgccc
      121 gcgatttcac ccgacgcaga attttgatcg ccgcgtccat gtattcctcg atcggccgcg
      181 agccgcgccg gtcgtccatc gtccccacaa agcagcgcat ggacgtcgcc cccaccgtat
      241 gcgccacccg cagcccgtcc gcgatcacct gctccggcgt cccctggttc ggcttccagc
      301 ttttcgacaa aggacagatg cacccgatcc ctgcgtcgat ctgaatcccg gccttcgccg
      361 catagtcgcg cacctcgcgc agatgcgccg cgtccgtgct cgaataatcg gccgaatccg
      421 aaatctgcag cgtgtcgcat ttcatccgcg cggcgaaatc gatcagttgc ttgtccttcc
      481 acccgaaggc gcgcagcgaa taggtgtcga accccagctt gatgcccgtc tccgcccgcg
      541 tggcttgcgc aagcgccggc agcgcggtgg cggtttcgag gaacgcgcgg cgattcatag
      601 ctgcatttgt agcacgatgc attggtagaa gataacgcca cgctcttcga agctaaactg
      661 aacggatact cttgccatgg ctcctccaac ggacgcaagc gccgcgctcg tcgtcgtcga
      721 tcccaatggc cgccgtcacc gcgccgctct cgcctcattc ccctttcgca tcgggcgcgc
      781 cgcggataac aatctggtgc tgcgcgacag ccgcgtctcg cgtaatcacg cccagatcct
      841 tttcgatggc tgccagttcg tcgtcgaaga tctgggcagc ctgcacggcg tgtgggtaaa
      901 cggcgagcgc gtcggcgtgc gccgtccgct cgatggttcg gaatccatcg agttcggcgt
      961 tccggacggt taccgtctcc acttcacacg ctcccgcgat gagctgcaga agcttctcgc
     1021 gcgtccgctg ccttccgata cttcgcgttc cggcggagcc aatctggaaa agctgcgcgc
     1081 tgtcctcgag gtcgcccgct cgctccaaag ctcgttctcg gtcgacgacg tcctcaatac
     1141 tctggtcgat gccgcccttg ctgtcaccgg cgccgaacgc ggattcctcc tgctcttcga
     1201 tgcggcgcat gagcttcaga cgcgcagcgc ccgctcgcgc ggcgggcgcg acctgccggc
     1261 cagcgatctc cgcgtcccct gccgcctcat ccagcaggcg ctcgaaggtc gtcgcgatct
     1321 cttctatatg tcgttcgatc cggcggcgct cgaatgcgct cccggcaata ccatctccgt
     1381 gctcgagctg cgcagcgtcg cctgtatccc gctcgtgcgc gtcaatctcc gcagggcgga
     1441 tacgcagccg ctgccccaaa gcgcggccaa cctgggcgtc ctctatatgg attcgcgcgg
     1501 cggcgctgtg gatctggccg gtggcaatcg cgagctgctg caaaccctcg ccattgaagc
     1561 ctccaccgtc ctcgaaaatg cccgcctgct cgaagaagag cgggttcgcc agcgcgtcga
     1621 agaggagatg gatctcgccc ggcgcatcca gcaaagcctc cttcccaccg ccctgcccaa
     1681 caccggatgg ttccgcgtct gcggatcgag cgaagcttcg catcaggtgg gcggcgacta
     1741 tttcgatgtc gcgcaaaaga gcgccggctg ctggtccctc gtggtggcgg acgtggccgg
     1801 caaaggcgtc ggctccgctc tgatggccag ctttctccag ggcgctttcc tgagcgcttc
     1861 gggcgccgag cccatcccgg agctgatggc gcgcatcaac gcctttctct ccgagcgcgc
     1921 cgaatacggc aaatacgcga ccatgttcta cgccacgctc gaccgcgcgg gagccctcac
     1981 gtacgccaac gccgcacagt gcaccccgta tctgctgcgc aaagacgccg cgcccaaaga
     2041 cagcaaaatc gagccgctcg aatccacctg ccgccccctc ggcctgctgc cggaaggcga
     2101 tttccccgcc gagcagcggc atctgcgcgc gggcgaccgg ctcatcgtct acagcgacgg
     2161 cgtaaccgag gcccgcaacg acgccggcga attct
//
LOCUS       DASZMC010000194         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2559953,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000194 DASZMC010000000
VERSION     DASZMC010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2559953"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VG274_03830"
     CDS             complement(<1..190)
                     /locus_tag="VG274_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05811.1"
                     /translation="MRRYGAWLWLATLVLTAAWLILRLSSGVSLQSNILALLPPTERD
                     ATAQSIQDRIAKAFSARVI"
     gene            complement(187..756)
                     /locus_tag="VG274_03835"
     CDS             complement(187..756)
                     /locus_tag="VG274_03835"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HWE05812.1"
                     /translation="MGNAGAAGPQLVKTGQILRGRFTLNRQLAGFAKPLRSEGSFSLI
                     PGRGLIWHAQTPFENVTVITSDGVSTRVNGQETMRLPAARMPGIGHLYEVLGGAVSGN
                     VALLQQDFSVNRTTDAAGWHLVLTPLHPDTLTISQIGSLAVAGHRFVESIEIDKAGGD
                     VDRMSFVGQTVETALPTAEERALLESLHK"
     gene            complement(954..>2916)
                     /locus_tag="VG274_03840"
     CDS             complement(954..>2916)
                     /locus_tag="VG274_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HWE05813.1"
                     /translation="TTCAPRCAAFAKVRLTVFFVAMFVSGVTCAAEPGWPFYGGDEGG
                     QRFSAAAQITPANVGGLQQAWSYSTRDLATKGAAIRHASFEVTPILDDGRLYLCSPFD
                     EVSALDPGTGHEIWRFDPKIDPRLRYPNSFICRGVAWWRDPKPKRGFCTARIFLATND
                     RRLIALDAASGKPCMGFGRGGTVAIEPAPAMIYKSEVQITSAPVVTQGLVIVGSSIDD
                     NQRVDAPRGTVHAYDARSGTLRWSFDPIPRDAVAPNAASWTSAGAARTGAANVWAPMS
                     VDEARGLVFVPTTSPSPDFYGGARPGDNRYADSVVALRVANGRVAWSFQVTHHDLWDY
                     DVPAQPTLGVVTWQDKTVPAVIQPTKQGLVFTLDRLSGEPLIPVEERRVPRGGAPGEQ
                     LSPTEPFPLAPKPLAPTRVKPDDAWGLTWYDRGQCRNKIAAARAQGLYTPPSLRGTIL
                     YPFTGGGTNWGGLAFDAAHDMVYVNTSSLMHLVTLIPKSELGAAERASPDAEISPQSG
                     TPFGMKREIILSPFGLPCNPPPWGMLHAIDMHDGRVKWEVPLGTTEDLLPLSQYFLGK
                     IGVPNLGGPIATAGGLVFIAATMDDYLRAFDARTGAELWRGRLPAGGQATPMTYVWKG
                     RQYVVIAAGGHEKLNTKRGDSVIAFALAR"
BASE COUNT          513 a          945 c          984 g          474 t
ORIGIN      
        1 agattacgcg cgcggaaaac gccttggcga tccggtcctg gatgctttgc gcggtggcat
       61 cgcgttcggt gggcggcagc agggccagaa tattgctctg aagcgaaacg ccgctggaca
      121 acctgaggat cagccaggcc gcggtcagca cgagtgtggc gagccagagc cacgctccgt
      181 accgcctcat ttgtgcaagc tctcgagcag cgccctctct tccgccgtcg gcaaagccgt
      241 ctccacggtc tggccgacga agctcattcg gtcgacgtcc ccaccggcct tgtcgatttc
      301 aatgctttcc acgaagcgat ggcccgcgac ggcgagcgat ccgatttgcg atatggtgag
      361 agtgtcggga tgcagcggcg tgagcacgag atgccatcca gccgcatccg tggtccgatt
      421 gaccgaaaag tcctgctgca acagcgcgac gttgcccgag acggcgccac ccagaacttc
      481 atacaggtgt ccgatcccgg gcatgcgcgc cgcgggcaga cgcatcgtct cctgtccgtt
      541 gacgcgcgtc gatacgccgt ccgaggtgat cacggtgacg ttttcgaagg gcgtttgtgc
      601 gtgccagatc aggccgcggc ctggaatcag cgaaaagctc ccttcgctgc gaaggggttt
      661 ggcgaaaccc gccaactggc gattcaaagt gaaacggccg cgcagtatct gtccggtctt
      721 gacgagctga ggcccggcag cacccgcgtt gcccaagccc ggggcgccga tgaaaaaggg
      781 aagggccgcg aacgcggcga gccatgcagc aatcgcgcgg gaatgcatcc gggtacgggc
      841 cgaagtgatt gctgttccgg cgcgattatg ggatggggcg ataaagcgat gcaacccgat
      901 ccgatggccc gcgcgacgcg cggccgcacg acaagctgaa gtgcgaagag ccgctacctt
      961 gccagcgcga acgcgatcac gctgtcgccg cgcttggtgt tgagcttttc gtggccgccg
     1021 gcggcgatga cgacatattg gcggcctttc cacacatagg tcatgggcgt cgcctggccg
     1081 ccggcgggca ggcggccgcg ccagagctcc gcgcccgtcc tggcgtcgaa ggcgcgcagg
     1141 taatcgtcca tcgtggcggc gatgaaaacg aggccgcccg cagtcgcgat cggtccgccg
     1201 agattgggca cgccgatctt tcccagaaaa tattgcgaaa gcggcagcag atcttccgtc
     1261 gtgcccagcg gcacttccca cttcacgcga ccgtcatgca tgtcgatggc gtgcagcatg
     1321 ccccagggcg gcggattgca gggcagcccg aagggcgaga ggatgatttc gcgcttcatg
     1381 ccgaagggcg tgccgctctg cggcgagatc tccgcatcgg gcgatgcgcg ttcggccgcg
     1441 ccgagttccg atttcgggat cagcgtgacc agatgcatca ggctcgacgt gttcacatag
     1501 accatgtcgt gcgcggcatc gaaggcgagc ccgccccagt tcgtgccgcc gccggtgaag
     1561 ggatagagaa tcgtgccgcg caacgacggc ggcgtgtaga gcccctgcgc gcgcgccgcc
     1621 gcgatcttgt tgcggcactg gccgcggtcg taccaggtga gcccccaggc gtcgtcgggc
     1681 ttgacgcgcg tgggcgcgag cggcttcggc gccagcggaa agggctcggt gggcgacaat
     1741 tgctcgcccg gcgcgccgcc acgcggcacg cgccgctctt ccaccggaat gagcggctcg
     1801 ccgctcagcc ggtcgagcgt gaagacgagc ccctgcttgg tgggctggat gacggccggc
     1861 acggtcttgt cctgccaggt gacgacgccg agcgtcggct gcgccggcac gtcgtaatcc
     1921 cacaaatcgt gatgggtgac ctgaaaggac caggcgacgc gcccattcgc gacgcgaagc
     1981 gcaacaacgg aatccgcgta acggttgtcg cccgggcgcg cgccgccata gaaatccgga
     2041 ctcgggctcg tggtcggcac gaagacgagc ccgcgcgctt cgtcgaccga catcggcgcc
     2101 cacacattcg ccgccccggt gcgcgccgcg ccggcgctcg tccagctggc cgcgttgggc
     2161 gccaccgcat cgcgcgggat cggatcgaag ctccagcgca gcgtgccgct tctcgcgtca
     2221 tacgcgtgca ccgtgccgcg cggtgcatcc acgcgctggt tgtcgtcgat cgaggagccg
     2281 acaatgacga ggccctgcgt caccaccggc gcggaggtga tctggacttc gcttttgtag
     2341 atcatcgccg gggcgggttc gatcgccacc gtgccgccgc ggccaaatcc catgcatggt
     2401 ttgccggaag ccgcgtcgag cgcgatcagg cggcgatcat tggtcgccag gaagatgcgc
     2461 gccgtacaga atccgcgctt cggtttcgga tcgcgccacc acgcgacgcc gcggcagatg
     2521 aaactgttgg gatagcgcag gcgcggatcg attttgggat cgaaccgcca gatttcgtgg
     2581 cccgttcccg gatcgagcgc gctgacttcg tcgaaaggcg agcagagata gagacgtccg
     2641 tcgtcgagga tcggcgtgac ctcgaaagag gcgtgccgaa tcgccgcgcc cttcgtcgcg
     2701 agatcgcgcg tggagtagct ccacgcctgc tgcagcccgc cgacattggc cggcgtgatc
     2761 tgcgcggcgg cggaaaagcg ctgcccaccc tcgtcgccgc cataaaaagg ccagcccggc
     2821 tccgcggcac acgtaacacc tgagacgaac attgccacga agaagacagt caatcgcact
     2881 ttcgcaaatg ctgcgcatct tggagcgcag gttgta
//
LOCUS       DASZMC010000195         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2560743,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000195 DASZMC010000000
VERSION     DASZMC010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560743"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..647
                     /locus_tag="VG274_03845"
     CDS             102..647
                     /locus_tag="VG274_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HWE05814.1"
                     /translation="MTRKPMLEALKLRGERKRIAECLCARISERAREPVFFRSFAVAD
                     TIDGRFDLVVLHAWLVLEVLEARREAALAQALVDALFARFDDALREQGAGDMGMNRRI
                     RKMAGAFYGRLRAYREAGSESALAAAISRNLYRGETSQVELAGMLAKYALDARAGLAS
                     LCLDRGHLDFGPLPAPAGTKA"
     gene            644..1174
                     /locus_tag="VG274_03850"
     CDS             644..1174
                     /locus_tag="VG274_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05815.1"
                     /translation="MTITAPLERFRDLADLPHAGYEGAIVASADELRRLAEWAGVDEV
                     TRLVGHVDIRPQSRTRFLLETEFEADIVQKSVVALKPLRSTIARSFARTLQLSPGLQR
                     HADKGGLVTSALADDEAPEEIDSTRFDLAGPLREELLLAIDPYPRAPGETFDLPGDAG
                     SPESPFAALEKLKRER"
     gene            1291..1512
                     /locus_tag="VG274_03855"
     CDS             1291..1512
                     /locus_tag="VG274_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05816.1"
                     /translation="MPVRFRIGTAAIFGAVLGIFLAFAGRLENGGKFLPSAGWWSGFV
                     IGGMVAGAVLFVTLAVIYNLVRAARSGSD"
     gene            1566..2105
                     /locus_tag="VG274_03860"
     CDS             1566..2105
                     /locus_tag="VG274_03860"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWE05817.1"
                     /translation="MNELPLPNVELDRTTDWPAVLSSVRDYWNGKRGSRAMPSRSDIS
                     PAQLKSFLPYILLADVVDGGKDFRYRVVGTQLREFFYFEPTGKLMSEALAPFGRETVQ
                     ATLAGYRAVIERRAPMRLTGSGSWFGQNPKYFDAMLAPMSDDGEAVNMILGTFIFVWD
                     RDHQFRAPFDPRFGGHVLA"
     gene            2264..2443
                     /gene="rpmF"
                     /locus_tag="VG274_03865"
     CDS             2264..2443
                     /gene="rpmF"
                     /locus_tag="VG274_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWE05818.1"
                     /translation="MAVPKRKTSPSRRNMRRSHHRLVPNAHIECPQCGEQKRPHHVCA
                     KCGHYGGREVVKVEA"
     gene            2496..>3081
                     /locus_tag="VG274_03870"
     CDS             2496..>3081
                     /locus_tag="VG274_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWE05819.1"
                     /translation="MSPQLTVSIDAMGGDAGPGVVLAALSRSLVRHPGVRFLLHGDEA
                     LLKPLLARRAKLAARAAVRHSAEHVRMEEKPGQALRRGRNTSMWRAIESVAKKEAEVA
                     VSAGNTGALMAMSMFQLGLLDGIDRPAIAVLWPTKRGQTVVLDVGANIATDSQQLVDF
                     AVMGEAFARAILGLERPIVGLLNVGAEDVKGNDAV"
BASE COUNT          528 a          957 c         1034 g          562 t
ORIGIN      
        1 gcaagaaccg gacgccgcat cgaagacacc tcgcacctga ccctgcgccc cgcctcccat
       61 gtgctagcgt cgagccggcg gcgttgcaag aggcggcgcc ggtgacgagg aagccgatgc
      121 tcgaggcgct caagctgcgc ggcgaacgca agcgtattgc ggaatgcctc tgtgcgcgga
      181 tttcggagcg cgcgcgcgag ccggtgtttt tcaggagttt tgcggttgcc gacacgatcg
      241 acggccggtt cgacctggtc gtgctgcacg cctggctcgt actcgaagtt ttggaggcca
      301 ggagggaggc ggcgctcgcc caggcgctcg tcgatgcgct tttcgcccgt ttcgacgacg
      361 ccttgcgcga gcaaggcgca ggcgacatgg gcatgaaccg ccgcatacgg aagatggcgg
      421 gcgccttcta tgggcgtctg cgcgcctacc gcgaagcggg cagcgaaagc gcgcttgccg
      481 ctgcgatctc ccgcaacctc taccgcggcg agacttcgca ggttgaactg gcgggaatgc
      541 ttgccaaata tgcgctcgac gcgcgcgcag gcctcgcgag tctctgcctc gatcggggac
      601 acttggattt tggtcccctg cctgcgccgg ccggaacgaa ggcatgacca tcacggcccc
      661 cctggaacgg tttcgcgatc tggccgatct gccgcatgcg ggttacgaag gtgcgatcgt
      721 cgcgagcgcg gacgaactcc gccgcctcgc ggaatgggct ggcgtcgacg aggtcacgcg
      781 cctcgtcgga catgtcgata tccggccaca atcgcggacg cgctttctcc tcgagaccga
      841 attcgaggcc gatatcgtgc agaaaagcgt ggtcgcgctg aaacccctgc gttcgacgat
      901 tgccagaagc ttcgcgcgca ctcttcagct gtcgcccgga ctgcagcgcc atgccgacaa
      961 gggcggactt gtgacatcgg ctttggccga cgacgaggcg ccggaagaga tcgatagcac
     1021 tcgcttcgat ctcgcgggcc ccctgcggga ggaattattg ctggcgatcg acccctatcc
     1081 gcgcgcgccc ggcgagacgt tcgatctgcc cggagatgcc ggttcgcccg agagcccgtt
     1141 tgcggcgctc gaaaagctca agcgcgagcg ctgacatgcg acatcgggcg cgcggggagg
     1201 cggcttccgt ttcatttacc aattcgtgaa acaaatcgaa ttatggggac ggaattcagg
     1261 cagccggatt cgaactgaga gcaggaagaa atgccggtca ggtttcgtat cgggacggct
     1321 gccatcttcg gagcagtgct ggggattttt ctggccttcg ccgggcgcct ggaaaatggg
     1381 ggaaaattcc tgccgagcgc cggctggtgg agcggattcg tgatcggcgg catggtcgcg
     1441 ggcgcggttc tgttcgtgac gctggccgtc atctacaatc tggttcgggc agcgcggtcg
     1501 gggtcggatt gagtggggtg ggctcggcgg ctttcgctcc ggcacgtgga actgagagag
     1561 catttttgaa cgaattgcca ttgccgaatg tcgaattaga ccgcacgacg gattggcccg
     1621 cggtgctttc ctcggtgcgc gattactgga acggaaagcg cggatcacgg gcgatgccca
     1681 gccggagcga tatttcgccc gcgcaactca aatcgttcct gccctacatt cttctggccg
     1741 acgtcgtcga cgggggaaag gactttcgct atcgcgtggt cggcacgcag ctgcgcgaat
     1801 tcttctattt cgagccgacc ggaaaattga tgagcgaggc cctcgcgccg ttcggccgcg
     1861 agacggttca ggccacgctc gccgggtatc gcgcggtcat cgaacgccga gcgcccatgc
     1921 ggctcaccgg ttcgggctca tggttcggac agaatcccaa atatttcgac gcgatgctgg
     1981 cgccgatgtc ggacgacggc gaggccgtga acatgatcct cggcaccttc attttcgtct
     2041 gggatcgcga ccatcagttc cgcgcgccgt tcgatccccg gtttggagga catgtcttgg
     2101 cttagccgga cagccccgcc cgataatccg gccgccttga atcccgggcg ggtttcctgt
     2161 tcactccgcg gctcgttttg cccgcccggt ccgccagtgg cagaagcgcc gaaagacgcc
     2221 gccccattcg gattcggctt cagtttgaag gagttgcacc gccatggcgg tacccaagag
     2281 aaaaacatcg ccgtcgcggc gcaatatgcg ccggtctcat caccggctgg tcccgaacgc
     2341 ccacatcgaa tgcccgcaat gcggcgagca gaagcggcct catcatgtct gcgccaaatg
     2401 cggccattat ggcggccgcg aagtggtgaa ggtcgaagcc tgacacgcac cgcccgcgcg
     2461 gaccgcgcgc gggggatggt cgcagcgagg cgagggtgag cccccaattg acggtttcga
     2521 tcgatgcaat gggcggcgat gccggtccag gcgtggtttt ggctgcgctc tcgcgctcgc
     2581 tcgtgcgcca tccaggcgtg cgttttctgc tgcatggcga cgaagccctc ctgaagcctc
     2641 ttctcgccag gcgcgcgaaa ctggccgcgc gcgccgcggt tcgccacagc gcggaacatg
     2701 tgcgtatgga ggagaaaccg ggtcaggccc tcaggcgcgg gcgcaacacc agcatgtggc
     2761 gggccatcga gtccgtcgcc aagaaggaag cggaagttgc cgtctcggcc ggcaatacgg
     2821 gtgcgctgat ggcgatgtcg atgttccagc tcggactgct ggacggaatc gaccggccgg
     2881 cgatcgcagt cttgtggccg accaagcgcg gacagaccgt ggtgctcgac gtcggcgcca
     2941 acatcgccac cgactcgcag cagcttgtcg actttgcggt gatgggcgag gccttcgcgc
     3001 gcgccatcct gggactcgag cgtccgatcg tcggcctgct caacgtcggc gccgaggacg
     3061 tgaagggcaa cgatgccgtg c
//
LOCUS       DASZMC010000196         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_78566,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000196 DASZMC010000000
VERSION     DASZMC010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78566"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1084)
                     /gene="istA"
                     /locus_tag="VG274_03875"
     CDS             complement(59..1084)
                     /gene="istA"
                     /locus_tag="VG274_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042551150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HWE05820.1"
                     /translation="MLKAEEQMELAVLKKHGASIRGLSRSTGRSRNTVRRYLRGGDAV
                     AKRKPAPKRAEKLDPFKAYIVGRMKAALPDRIPATVLFREIKERGYQGGETRVKLFVR
                     GLTPLPAAAPAVRFETTPGHQMQADWATVGRGADKLSLFIATLGWSRQAYVEFCDDER
                     VETLIGCHETAFLTFGGVPVEVLYDNMKTVVIERNTYGLGVHRFHAGFLDYARHAGFL
                     PRLCQPYRAQTKGKVERFIGYLKRSFWVPFVASMRQAGLKPDKHAANGAVGRWLREVA
                     NARVHATTNAVPAERLVIEQAALQALPAPYRGRSPRSLLTPVRKAVVGYQHPLAVYED
                     LMPGGAA"
     gene            complement(1544..>1803)
                     /locus_tag="VG274_03880"
     CDS             complement(1544..>1803)
                     /locus_tag="VG274_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWE05821.1"
                     /translation="LQEHLVKRERLRAVANQFGVDIRTAALQFSAAPDVAVSLIVGAH
                     SEAQALANASSMKVTIPQAFWAELKRQNLIEQSAPVPEGGA"
BASE COUNT          336 a          619 c          534 g          314 t
ORIGIN      
        1 agtgccgtca gccgcaggtc ctgcgccagg accgtgatgc gctcgtgctg gagattgttc
       61 atgccgcgcc tcccggcatc agatcttcgt aaaccgcgag cggatgctga tagccaacga
      121 cagctttgcg cactggcgtt agaagactgc gcggcgaacg gccgcgatag ggcgcgggaa
      181 gtgcctgcag cgccgcctgc tcgatcacga gccgttcggc gggcaccgcg ttggtcgtcg
      241 cgtgcacgcg cgcgttcgcg acctcgcgaa gccaccgccc aactgctcca ttcgccgcgt
      301 gcttgtcagg tttcaggcca gcctgtcgca tcgacgcaac aaacggaacc cagaagctgc
      361 gcttcagata gccgatgaag cgttcgacct tgcccttcgt ctgcgcgcga tacggctgac
      421 acagtcgcgg caggaatcct gcgtgccgcg cataatcgag gaaccccgca tggaagcgat
      481 gcacgccaag cccataggtg ttccgctcga taacgacagt tttcatgttg tcgtaaagca
      541 cctcgaccgg cacgccccca aaggtcagga atgcagtctc gtgacagccg atcagcgtct
      601 cgacccgctc gtcgtcgcag aactctacat aggcctggcg gctccacccc agcgtcgcaa
      661 tgaagagcga tagcttgtct gcgccgcgcc caaccgtcgc ccagtccgcc tgcatctgat
      721 gaccaggcgt ggtctcgaag cgaactgcag gcgctgcggc tggcagcggc gttaatccac
      781 gcacgaacag cttcacacgc gtctcgccac cctgatagcc acgctctttg atctctcgaa
      841 ataacactgt cgccggaatg cggtcaggca gcgcggcctt catccgaccg acgatgtaag
      901 ccttgaacgg atcgagcttc tcggcccgtt tcggcgcagg cttacgcttg gccaccgcat
      961 cgccaccgcg caaataccgc cgaaccgtgt tgcgagaccg tcccgtcgac cgcgacagac
     1021 cccggatgct cgccccgtgc ttcttcaaaa ccgccaattc catctgctcc tccgccttca
     1081 gcatcggccc ctccctcacg gaggaaccac ctaatgcgat gggtcagatt cagggtgccg
     1141 ggcgggtcag tttcccactg ccgatgacac gatcgggcgc cgcgcgcggc agccacgcgc
     1201 caagggataa agagcaccat cccgtcagcc tgaaaatcca gagtgagcac acggcgtaga
     1261 gccctcaccg cggggttgcg atcggagtgt cggcgacctt gacccggtag ctccgaggcc
     1321 ggcgccgcca accgaattcc ccaaatagga atcggcggcg ccatcgccgc cagctcgggc
     1381 atgatcgggc gacgcccctc accgcggggt tgcgatcgcg gtgtcggcga ccttgacccg
     1441 gtagctccga ggccggcacc gccaaccgcg cgtgcgcgag accacagctc gcgcgcgcgt
     1501 gagagcagcg ggtagggtca acgctgatcg gcacggcctt cggctaggct ccgccttccg
     1561 ggacaggcgc gctctgctct attaggttct gccgtttaag ctcggcccag aaggcctgcg
     1621 gaatggtgac cttcatcgaa cttgcgttcg ccagcgcctg cgcctcgcta tgcgctccga
     1681 cgattagtga aaccgcgaca tctggcgctg cggaaaattg caatgctgct gttctgatat
     1741 caacaccgaa ctggttggcc acagcccgca gccgttcgcg cttgacgaga tgctcctgca
     1801 aga
//
LOCUS       DASZMC010000197         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1735256,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000197 DASZMC010000000
VERSION     DASZMC010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735256"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VG274_03885"
     CDS             <1..699
                     /locus_tag="VG274_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWE05822.1"
                     /translation="IRERIIRGDIAIGQVISRRKIAADLGVSFLPVSEAFLRLEFEGL
                     LESRPRAGTRVRIPTRADIEGHYVVREGLELQAARLFAERATPEARIELLKLAQRVDA
                     LAAQPDTDRFVHLSTHEKLHRRIAEGAQCPILSDTIDKTCALSSTWLCSGREMQGNMP
                     RSSHTYLAETLVKGDPEAAIVAMREHIRISKESAMERLKPYFELAERQTQRYSRTPSV
                     RPPLAPTRPDARVS"
     gene            complement(721..1944)
                     /locus_tag="VG274_03890"
     CDS             complement(721..1944)
                     /locus_tag="VG274_03890"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 family protein"
                     /protein_id="HWE05823.1"
                     /translation="MCRNIRPLFNFDPPSTEEEVHAAALQFVRKISGFTKPSQANEDP
                     FIAAVEDITAVSRRLIAALETNSPPKNREGEAAKAKARNAQRFGVISAVALAMLAAPH
                     AYGQLRDVTVAAIPGVIAQGAKWSVAWQGTDNADGIVGTPDGDLLFAQEQPNRISKLD
                     QSAHATPFLEDTHGAGAISIGKKGQIFAVERTCTDPGRKGPECNEPTAIAMFAPQRKK
                     LADNIDGKSLGRLNDLIVSKRGDVYFTSGGLFRADPAGKVTAIGMSLRTNGIMLSRDE
                     KILYVTNGGEVVAFDIQPDGSAANQRAFGKLEAGGNGDAMAIDAEGRLYVTSNPGVQV
                     FATDGKYLGVIPTPRPVISVAFSGARKKSMYVVGAGALAPGGGEFQTPEGVRNNAKTI
                     FKIETIAQGFKGRAK"
BASE COUNT          461 a          725 c          730 g          516 t
ORIGIN      
        1 atccgcgagc gaatcatccg cggcgatatc gccataggcc aggttatttc gcgccgcaaa
       61 atcgcggccg atctaggcgt gagctttttg cccgtttccg aggcattcct ccgtctggaa
      121 ttcgaaggat tgttggaaag ccggccgcgg gcgggcacgc gtgtccgcat cccgacgcgg
      181 gccgatatcg aaggccatta cgtagttcgt gaaggcctgg aactgcaagc agcgcggctg
      241 ttcgccgaaa gagcgacgcc cgaagcccgg atcgagctct taaagctggc gcagcgcgtg
      301 gatgcgctgg cggcgcagcc tgacaccgat cgcttcgtgc atttgagcac gcatgagaaa
      361 ttgcaccggc gcatcgccga aggcgcgcag tgccccatcc tcagcgatac catcgacaag
      421 acgtgtgcgc tgtcgtcaac gtggctgtgc tcgggacgtg agatgcaggg caacatgcct
      481 agaagcagcc atacgtattt ggccgaaaca ctggtcaagg gcgatccaga ggcggcaatc
      541 gtcgccatgc gcgagcacat ccgcatcagc aaggaatccg cgatggagcg cctgaagccg
      601 tacttcgaac tggccgagcg gcagacgcag cgctattcgc gcacacccag cgtacgtccg
      661 ccactggctc ccacaaggcc ggacgcgcgg gtttcgtagc gcgacgcctg attgaacctg
      721 ttacttcgcg cggcctttga atccctgtgc aatggtttcg attttgaaaa tcgtcttcgc
      781 gttgttgcgc acaccttcgg gcgtttggaa ttccccgccg cctggcgcaa gcgctccggc
      841 gccgacaacg tacatgcttt tcttcctggc ccccgaaaaa gccacgctga tcacaggccg
      901 cggcgtcgga atcacgccga gatattttcc atccgttgcg aatacctgca cgccgggatt
      961 cgacgtgaca tagaggcgtc cttccgcgtc gatcgccata gcgtcgccat tgcctccggc
     1021 ttccagtttc ccgaatgcgc gctgattcgc agccgagccg tcaggttgaa tgtcgaacgc
     1081 gacgacttcg ccgccattgg ttacataaag gatcttttca tcgcggctta gcatgatgcc
     1141 gttggtgcgc aagctcatgc cgatcgccgt cacttttccc gcaggatcag cgcggaaaag
     1201 tccgccggag gtaaagtaga catcaccacg tttgctcacg atcaaatcgt tcaagcgccc
     1261 gagcgatttg ccgtcgatgt tgtcggccag ctttttacgc tgtggcgcga acatcgcgat
     1321 ggcggtgggc tcgttgcact cgggtccttt acggcccgga tcggtgcagg tgcgctcgac
     1381 ggcgaatatc tgtccttttt tgccgatact gatggcaccc gcgccgtgcg tgtcttcgag
     1441 aaacggcgtc gcgtgagcgc tctggtcgag cttactgatg cgattgggct gctcctgcgc
     1501 gaacaacaaa tcgccgtcgg gcgtgccgac gataccatcg gcgttatcgg tgccctgcca
     1561 cgcaacggac cacttcgcgc cctgcgcgat cacgccggga atcgccgcga cggtgacatc
     1621 gcgcaattgc ccgtaagcat ggggagcggc gagcatcgca agcgcgactg cggaaatgac
     1681 accgaatcgc tgcgcgttgc gagccttggc cttggcggct tctccttcgc gattcttcgg
     1741 cggcgaattc gtctcgagtg ccgcgatcaa gcggcgcgag acagcagtga tatcttccac
     1801 cgccgcaatg aacgggtctt cattggcctg cgatggttta gtgaagccgc ttatcttacg
     1861 gacgaattgc agagcggcgg cgtggacttc ttcttctgtc gatggcggat cgaaattgaa
     1921 tagcggacgg atgttgcggc acataaagat gctctcgaag ttaccagaat gatccgcgca
     1981 ggccggtggc gtcaaatccg atcggcggtg actggatttt aggagcgcgt gcaggggaca
     2041 ctgcggatcg ggcgagtggc tccacacgca cgcgcgggtc ttttcaccgc tccgtggaaa
     2101 gggccgattt tctgaaaaac cgaaatgccc attgcggggc cgctaggggg cctgcacgcg
     2161 atcgaagctc gtcccctagc ttcggtattc cccggaccct atcaatcgcg tggcgcgtgt
     2221 ttctgcgcgg tatttttttc taaacgttta tgtgtctgat ttaagtcgat ttgttgtcgc
     2281 gcgcgccgtg ccgatggcac attcttcacg atttgcaatc acgcaagcgg gaaaagtttt
     2341 tctcacttcg cgacaatcat ttcgcgcgtg ccctcgtggt gcaaacccag aatgcgtgca
     2401 tcgagtgtcg tccgaagacg ccgcaacgcg cg
//
LOCUS       DASZMC010000198         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5026068,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000198 DASZMC010000000
VERSION     DASZMC010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5026068"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /gene="solA"
                     /locus_tag="VG274_03895"
     CDS             <1..1191
                     /gene="solA"
                     /locus_tag="VG274_03895"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWE05824.1"
                     /translation="CRPAACPTRPMKVFDALVLGLGALGSAVLLQLARRGVRVLGLDR
                     LAPPHTRGSSHGETRITRCAVGEGEHYSPLALRSHVLWREIESETGADLLTTQGVLVL
                     SSPARTSFTHVENFFANTVAAARKYHIAHEILVAEAIRRRFGQFRIRDDEIGYFEPGG
                     GLLRPEVCLSAQLELARRHGAEIHVNERVLSFAPRRADVVVTTDRDTYVAGRLVVAAG
                     AWLPELLDANLARHFRIYRQAQFWFADPAGLFGAGRFPVFIWELSGRRQSIYGFPDID
                     GGGVKIATEQFASTTTADVPESEVGASESAAMHATYVAPFLPQLSATCTRASTCLYTV
                     TADFGFVVDQHPQSDRVILASCCSGHGFKHSPALGEAIAELIVNGRSQTDLGSFKLAR
                     LSAH"
     gene            1310..2746
                     /locus_tag="VG274_03900"
     CDS             1310..2746
                     /locus_tag="VG274_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWE05825.1"
                     /translation="MSFRAPVPKPQTSRPGFLRLLAILTDNPLEAWTAEHFEKPLVHD
                     RLPFMPAVVVSDPSAVQRILLDNAQNYRKDKLLHRILSPTLSNGLLLVDGEQWRKQRR
                     AVAPMFARKSVASYAAAMKDAADAVVGRWLRNPDESIVDVAQEAAEATLDVLQRTIFS
                     QGIGRSTRDFRLHMRRYFDAIGRLDPCDVLALPAFLPRWTKWRARDSMRFFDAAVDAV
                     IRQRRTLASQQDDERLNDILNLLLEARDPETGEGLNEEELRANIVTLIAAGHETTANA
                     LTWSLFLLSQDKVWQDRVAAEAQRTVAGAAEWDPDGLASTRAVVEEALRLYPPIAAIS
                     RVAIAADELSGQPIRAGTMTIVAPYVIHRHRRLWERPDAFDPNRFLGENRMRVNRYAY
                     LPFGAGPRICLGASFALQEATILLASIAAHFSFEMAPGTEIEPLLRITLRPRNGLPMV
                     LTRRPTANFRATVCRPARGQRQRIPMAS"
     gene            complement(2691..3101)
                     /locus_tag="VG274_03905"
     CDS             complement(2691..3101)
                     /locus_tag="VG274_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWE05826.1"
                     /translation="MCAAARNVTSDDVLKERDKLSLALKDRVDQVTEPWGVEIQSVEM
                     RNVEIPATMQRAMAQEAEALREKRARIIKAEAELEAARKLREAADEIMKTPASLELRR
                     MQMLTEVGAEQNSMTIVMMPSEFVAAARALADKP"
     gene            3215..>3439
                     /locus_tag="VG274_03910"
     CDS             3215..>3439
                     /locus_tag="VG274_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWE05827.1"
                     /translation="MLELYFWPTPNGKKVTILLEELELPYALKPTHIGRGDQFRPEFL
                     RISPNNRMPALVDTEPKGGGNPLAIFESAAI"
BASE COUNT          614 a         1128 c         1029 g          668 t
ORIGIN      
        1 tgccgtccag cagcctgccc aactcgcccg atgaaagtgt tcgacgccct ggttctcggc
       61 cttggggcgc tgggaagcgc cgtgctgctc cagcttgccc gccgcggcgt ccgcgtgctc
      121 ggcctcgacc gcctggcccc gccgcacacg cgcggctcgt ctcacggcga gacccgtatt
      181 acgcgatgcg ccgtcggcga gggtgagcat tattcgccgc tggcgctgcg ttcgcacgtt
      241 ctgtggcgcg agatcgagag cgagaccggc gccgacctgt taacaaccca gggtgttctt
      301 gttctctcaa gcccggccag gacaagcttc acccatgtcg agaacttttt cgcgaacacg
      361 gtagcggccg cccggaaata tcacattgcc catgagatat tggtcgcgga ggcaattcgc
      421 cggcgcttcg gccagtttcg aattcgcgat gacgagatcg gctacttcga gccgggtgga
      481 gggttgctgc ggcctgaagt ctgcctttcc gcacagctcg aactggctcg acgccatggc
      541 gcggagattc atgtcaacga acgcgttctg agcttcgcgc cgcgccgggc cgatgtcgtc
      601 gtcacgaccg acagagacac ctatgtcgca ggacgcctcg tggtggcggc aggggcgtgg
      661 ttgccggagc tgctcgatgc caacctcgcg cggcacttcc gaatctatcg ccaggcccag
      721 ttctggttcg cggaccccgc agggttgttc ggcgcgggcc ggtttcctgt cttcatctgg
      781 gaattgagcg gccgcagaca atccatctat ggatttccgg acatcgacgg cggcggcgtc
      841 aagattgcga ccgaacaatt cgcatcgact acgacggcag atgtccccga aagcgaggtt
      901 ggcgcgagcg aatctgctgc catgcacgca acctatgtcg cacccttcct gccgcaattg
      961 agcgcaacct gcacgcgtgc atcaacctgt ctatacaccg tcacggcgga tttcggattc
     1021 gtggtcgacc aacatccgca gagcgaccgg gtgatcctcg cctcctgctg ctccggccac
     1081 ggcttcaagc attctccggc gctgggtgag gcgatagccg aactcatcgt aaacggccgc
     1141 agccaaaccg atctcggatc gttcaaactc gcacgattat ccgcacactg acagtaggcg
     1201 attcctcgcc gccgctccgg caggaacgat gcccgcggcg tgccgttatc aatctggcct
     1261 gcaaccctga gcccgtgcag ccggaaatgc aaaacgcgac cgccacacaa tgagtttccg
     1321 cgcacccgtg cccaaaccgc agacgtcacg gccaggcttc ttgcggttgc tagcaattct
     1381 tacggacaac ccgctcgaag cctggacggc agagcatttc gaaaagcccc tggtgcacga
     1441 ccgccttccg ttcatgcccg ccgtcgtcgt cagcgatcct tcggccgttc aacggatact
     1501 gctcgacaac gcgcagaact accgcaagga caagcttctt caccgcatcc tttcgccgac
     1561 gttgagcaat ggacttctgc tggtggatgg cgagcaatgg cggaagcaac ggcgcgccgt
     1621 tgcgcccatg ttcgcgcgca aatcggttgc gagctatgcg gcggcaatga aggacgccgc
     1681 cgatgctgtc gtcggccgct ggcttcgcaa ccccgacgaa agcatcgtcg atgtggcgca
     1741 agaggcggcc gaagcaacgt tggacgtgct ccagcggacc atattttctc agggtatcgg
     1801 ccgcagcacg cgggacttcc gcctccatat gcggcgctat ttcgatgcga tcggaaggct
     1861 cgatccgtgc gacgttctgg cgttgccggc gttcctgccg cgctggacga aatggcgcgc
     1921 gcgcgattcg atgcgcttct tcgatgcggc ggtggacgcg gtcatccggc aaaggcggac
     1981 gctcgcctca caacaggatg atgaacgtct caacgacata ctgaatctgc ttctcgaagc
     2041 gcgtgatccc gaaacgggtg agggattgaa cgaagaggaa ctgcgcgcca acattgttac
     2101 gctcattgcg gcgggccatg aaaccaccgc aaatgctctc acctggtcgc tcttcctgct
     2161 gagccaggac aaggtctggc aggatcgcgt tgctgctgaa gcgcagcgga cagtcgcagg
     2221 cgccgcggaa tgggatcccg atggcctcgc ctcgacgcgc gccgtagttg aggaagcgct
     2281 ccggctatac ccgcccatcg ccgccatcag ccgcgtcgcc atcgccgcgg atgaattgtc
     2341 cggtcagccg attcgcgccg gcacgatgac gatcgttgcg ccttacgtga ttcaccgcca
     2401 tcggcgcctt tgggagcgtc ccgacgcgtt cgaccccaat cgcttcctcg gcgagaatcg
     2461 gatgcgcgtg aatcgatatg cctatctgcc atttggcgcc ggcccgcgaa tctgcctcgg
     2521 cgcatcgttc gcgcttcagg aggccacgat tcttctcgca tcgatcgccg cccatttctc
     2581 gtttgagatg gcccccggaa ccgagatcga gccgcttctg cgcatcacgc tgcggccgcg
     2641 caacggtttg ccgatggtgc tgacgcgccg gcctacggcg aatttccgcg ctacggtttg
     2701 tcggccagcg cgcgggcagc ggcaacgaat tccgatggca tcatgacgat cgtcatgctg
     2761 ttctgctccg cacccacttc ggtcagcatc tgcatgcgcc gcagttcaag cgaggcgggc
     2821 gtcttcatga tctcgtcggc ggcttcgcgc aatttgcgcg ccgcttcgag ttcggcttcc
     2881 gccttgatga tgcgcgcgcg cttttcgcgc aacgcctcgg cttcctgagc catcgcccgc
     2941 tgcatggtgg ccgggatctc cacattgcgc atctcgacgc tctgaatctc gacgccccaa
     3001 ggctcggtta cctggtcgac gcggtccttg agtgcaaggc tcagcttgtc gcgttccttg
     3061 agcacgtcgt ccgaagtgac gttccttgcc gcggcgcaca catcgatgtg acgccaaaac
     3121 gcatggcccc ctcttgtgca agcgggaatg aatcgcgaga ttcggacgtc caggggatag
     3181 gaacggggcg ccgcagcttt gcaggagcag cgcgatgctt gaactttact tctggccgac
     3241 tcccaacgga aagaaggtga cgatcctcct tgaggaactg gagcttccat atgcgctcaa
     3301 gcccacccat atcggacgcg gcgaccagtt tcgtcccgaa ttcctcagaa tctcgcccaa
     3361 caaccgcatg ccggcgctcg tggataccga accgaagggc ggcggcaatc cgctcgcgat
     3421 cttcgaatcg gccgcgatc
//
LOCUS       DASZMC010000199         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4479207,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000199 DASZMC010000000
VERSION     DASZMC010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4479207"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..856)
                     /locus_tag="VG274_03915"
     CDS             complement(251..856)
                     /locus_tag="VG274_03915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWE05828.1"
                     /translation="MKFVPFAAALVLACSTAQLADAQEVHMQTSLGEIDIALDAQHAP
                     KTVAIFLRYVREGHFDGTMIYRVVPGFVLQMGSYDTKGNPRPTHDPIPLESGNGLLNA
                     RGTLSMARSDDATSATAEFFINLSDNGDLDPKEGKAPNTTGYAVFGKVVEGMGVVDTI
                     AAVPLKGGQGPFPDNDPAQPVVIKSMTVVGDPPRAAAPAKH"
     gene            1181..1600
                     /locus_tag="VG274_03920"
     CDS             1181..1600
                     /locus_tag="VG274_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05829.1"
                     /translation="MNRVRSFYLGASALVALAASSAMTVDAAAPAMDNPFAQPSTLPL
                     HAPPFDKIKDTDYQPAMEEGMKQQLAEIDAIANNKAKPTFENTIIAMEKSGRMLDRVT
                     SAFFGVVQANTNDTLDKVQADEAPKLSDHNDAINLND"
BASE COUNT          327 a          498 c          509 g          335 t
ORIGIN      
        1 gtccggggta taggcgtggc agatgccggc gccggtggtc gaaccgagcc ccttccggtc
       61 gcgtgagttg cgcttggccg cgccgctgga ggcgcaagag gcgtcgtatt tcgcggcgtc
      121 ggcgaggatt tccagctttt ccagcaagga catcggcatg gctcgagcat agttcttctt
      181 ttgttctttt caagcacatc gttcgtccgg tggcgaacct tgcgtttgcg ctgtcagcga
      241 aatttagagc tcagtgcttt gcgggcgctg cggcgcgcgg cggatcgccg acgaccgtca
      301 tgctttttat cacgacgggc tgtgcgggat cgttatccgg gaacggtccc tgaccgcctt
      361 tcagcggaac cgctgcgatg gtgtcgacga cgcccatgcc ttcgacaact ttgccgaaca
      421 cggcgtagcc ggtggtgttg ggcgccttcc cttctttcgg atcgagatcg ccgttgtcgg
      481 agaggttgat gaagaactcg gcggtcgcgc ttgtcgcatc gtcgctgcgc gccatcgaca
      541 acgtcccgcg cgcattcagc aggccgttgc cggactcaag cggaatggga tcatgcgtcg
      601 ggcgcggatt gcccttcgtg tcataacttc ccatctgcag cacgaagccg ggcacgacgc
      661 gatagatcat cgtgccgtcg aaatgccctt cacgcacata gcgcaggaaa atcgccaccg
      721 tcttcggcgc gtgctgcgca tcgagcgcga tatcgatctc gcccagcgag gtctgcatgt
      781 gcacctcctg cgcgtcggcc agttgtgcgg tcgagcaggc gagaaccagt gcggcggcaa
      841 acgggacgaa cttcatcgag gaatttcctt atgtgatgac cggcatctat gcgggggatt
      901 ttggcgatcc tgtggccact agtcgggatt tggaatagcg aatcctggtt agcgcggaca
      961 taaagaacga tacagagccc gtgtgccgca agagaaatgc gcgcatgact gcgggttaaa
     1021 cccggcagca acactgtcac ccaaaagtga cttttgcgcg ccttgcagac ccgcatcccg
     1081 cgccttattt cggcttggct ggcgtgaaac agaaccgtta cagtccgcca aatttttttg
     1141 cgcggcgcgc aatttttcat ccttccaagg ggagttttcc atgaatcgag tacgcagctt
     1201 ttatctgggc gcaagcgcgc tggtcgcact cgcggcgagt tccgcgatga cggtcgatgc
     1261 cgcagcgcca gcgatggaca atccgttcgc acagccgagc accttgccgc tgcacgcgcc
     1321 gcccttcgac aagatcaagg acacggacta ccagcccgcg atggaagagg gcatgaagca
     1381 gcagctcgcc gagatcgatg cgatcgccaa caacaaggcg aagccgacct tcgagaacac
     1441 gatcatcgcg atggaaaagt ccggccgcat gctggaccgc gtgacgtccg ctttctttgg
     1501 cgtcgtgcag gccaacacca acgatacgct cgacaaggtt caggccgacg aagcgccgaa
     1561 gctgtccgac cacaacgacg cgatcaacct gaacgactag ctctttcagc gcgtgaaggc
     1621 cgtctacgat gcgcgcgaca cgctgaagct cgatccggaa tcgatgcag
//
LOCUS       DASZMC010000200         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4217148,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000200 DASZMC010000000
VERSION     DASZMC010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4217148"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..>1672
                     /locus_tag="VG274_03925"
     CDS             402..>1672
                     /locus_tag="VG274_03925"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HWE05830.1"
                     /translation="MHGSAVPLTAPRAAPAQQPKEQLASLAPGGSQGQSRVVIESVTP
                     WIDCGRFPAKGSVGEAVRVEADIFVDGHDRISAAIKHRITQRDTWHETDMTPGVNDRW
                     TAEFEVHAVGWHEFTVLAWVDRFATWRSDLQKRVAAAQNVSVDLIIGAELIDEAASAI
                     DGEVSRSLANFAGLLRSDDSGEAIEVALGGSLQTLMRRHAPRKFLTEWPTPFKLWVDR
                     PQARFSTWYELFPRSASPDPTRHGTLRDVQDRLDSIRTMGFDVLYLPPVHPIGRAFRK
                     GRNNSPAAEPGDVGSPWAIGGPEGGHDAIHPQLGTMEDFDGLVSAANERGIEIAMDLA
                     FQCSPDHPYVREHPEWFRHRPDGSIQYAENPPKKYQDIYPFDFECQDWQALWNELLRV
                     TLFWREHGVRIFRVDNPHTKPFPFWEWLIAQV"
BASE COUNT          319 a          525 c          517 g          311 t
ORIGIN      
        1 ggcgacctgt atgatttctc cgggcgtgaa cgtaacctgc gcgaacttca gacgcggcac
       61 ggcagagtcg taaaactctc gaatctcgcg tgacgcgggc tggatacgct ttcgcccatg
      121 gtgtaattcc ccgagatcag caaacagatc acatcgtatg tgactcgaca tgctgccgcg
      181 cggatcgttg ggtaaccacc acccgtaacc agtccagacc aagtggtatg cgatgacgag
      241 ggggagcgcc atggcgggaa gcttaactgg aggttcctga ggcggcgaga tccttcgttc
      301 gcgaaaccgc aagcggcggc ttcgggcggc acttagggga tggagcatca cataattccg
      361 acgctcccag ttgcagggga ccgtcctata ctgccgcgca tatgcacggg tccgccgtac
      421 cattgaccgc gccgcgcgct gcgccggcac agcagcccaa agagcaactg gcttcgctcg
      481 caccgggcgg atcgcagggc cagtcccgcg tcgtcattga aagcgttaca ccgtggatcg
      541 attgcggccg attcccggcc aagggttcgg tcggcgaagc cgtccgcgtt gaggcggaca
      601 ttttcgtgga tggccacgac cggatcagcg ccgcaatcaa acaccgcatc actcagcgcg
      661 acacctggca cgaaaccgac atgacgcccg gcgtcaacga ccgctggaca gccgagttcg
      721 aagtccatgc cgtcggctgg catgagttca cggtcctggc ttgggtcgat cgctttgcca
      781 cctggcgatc cgatctgcag aagcgcgtgg cggctgcgca aaacgtgtcg gttgatctga
      841 tcatcggggc cgagttgatc gacgaagctg catcggcgat cgatggcgag gtcagccgtt
      901 cgctggcgaa cttcgccggc ctgcttcgat ccgatgacag cggcgaagcg atcgaagtgg
      961 cgctgggcgg atcgcttcag accctgatgc gccgccacgc cccgcgcaaa ttcctgaccg
     1021 agtggcctac tcccttcaag ctgtgggtgg atcgtcccca ggcgcgtttc agcacctggt
     1081 atgaactctt cccacgctcg gcctctcccg acccgacgcg gcacggcacc ttgcgggatg
     1141 tgcaggaccg gctcgattca atccggacga tgggcttcga cgtgctttac ttgccgccgg
     1201 tgcatccgat cgggcgcgct ttccgaaagg gacgcaacaa ctcgcccgct gccgagccgg
     1261 gtgacgtcgg cagcccgtgg gccatcggtg gtcccgaggg cggtcacgat gccattcatc
     1321 cacaactcgg cacgatggag gatttcgatg gcctcgtcag cgcagcgaac gagcgcggga
     1381 tcgagatcgc aatggatctg gcgttccagt gctcgccgga tcatccgtat gtgcgcgagc
     1441 accccgagtg gtttcgccat cggcccgatg gctcaatcca atacgccgag aacccgccga
     1501 agaaatatca ggacatttat ccattcgact tcgaatgcca ggactggcag gcgctgtgga
     1561 acgaattgct ccgcgtgacg ctcttctggc gcgaacatgg tgttcgcatt ttccgcgtgg
     1621 acaacccgca caccaagccg ttcccattct gggaatggct catcgcgcag gt
//
LOCUS       DASZMC010000201         8033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_903516,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000201 DASZMC010000000
VERSION     DASZMC010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903516"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VG274_03930"
     CDS             <1..365
                     /locus_tag="VG274_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05831.1"
                     /translation="GPSLPFAYTDWGEMLLAKGDYNAAIDKFRDADLKGPHFADPLEM
                     WGEALMAKNRSDLALAKFEEANKYAPNWGRLHLKWGEALMYVGKRDEAKDQFAAAGRL
                     DLSSTDTAELARVKAPHG"
     gene            358..2325
                     /locus_tag="VG274_03935"
     CDS             358..2325
                     /locus_tag="VG274_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05832.1"
                     /translation="MAEDFESEEAGIRPAGRASDSLAAHLAIKTAGESSEEAREFLRS
                     QCRLADLQTENLRLQNENLGKQDEYELSHLRWRRFNDQMRGAMHMVLAALGALAVIGI
                     CAAVWNASRAQGFVIEAFSVPPGYAQRGMAGDVVAGDITDKIASIRQFANLNSFSRSK
                     EVSEDRKENVRVEIPETAISVAEAWRYLRNWLGDERHLTGSLRDTGAGQATLTLSAPG
                     AANVSVSGAAADLARLEAQAAEQMFGRIDPVNYVNYLTAKGRHREALAAAKHYASIAV
                     GIPERSDSFALWSYTTFEATGDFALAIQRARIGIGIDPKLAATRVQLMNEYFYLGRYE
                     DALREARPVLTLNESDQLAAHRSGGFAAMLVLSAGRAAYMLGDFADAASSRCGLGCAY
                     PEQALSRATNLAREHDVSNSRDLIASALAAGASDGGALGQARYYADAAADDWQAAVAE
                     AKAAAAAYAEPKGNLSGKYIATVEATRFAPPLAVAEAHTGDFATAHSVVDATPADCYE
                     CALARGNIDAIEKQSGAASSWFARTIALGPSIPFAYADWGEMLLADGNYDAAIAKFRL
                     ANQKGPHFADPLEMWGEALMAKNRSDLALAKFEEANKYAPNWGRLHLKWGEALFYAGQ
                     KTEAEKQFAVASQLDLSQPDKAVLAKLVNHG"
     gene            2318..4246
                     /locus_tag="VG274_03940"
     CDS             2318..4246
                     /locus_tag="VG274_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05833.1"
                     /translation="MAEELVRESEHSPRQRSDAVAWNILGEASREKADQFLDRQGALS
                     QLQMENLQLENKRLNAQHAFEVSHLRFRRFSDYARAALEGAGFLVILLLVCGLGTMVW
                     SATQDHDLVVDAFLVSPDVAQSGMTGSVLAGRVLDAFGRMQSSIGISVVQGAGSYHAG
                     YSEEVRVEIPQTGISIGELNRYLREWLGHETHVTGDLVHAAKGYALTTRASGQPGVTI
                     ESADMDALVRKAAEHMFAVVRPLRYLDYLEANNRVREALAAVVPLTKAGDAHDRAVAY
                     AAWAGLLSDSHDPDSALAKARMAASIDPQNPTAMGWLASIERTLGHDEAAWSLSSRNI
                     GLWHGAEVADLDPGLVATAPAFFTYRRDASIGDWAAVLTAEADFADGSGYSTPANRAF
                     EAAHDHDVALALSYVKSIPLRANSVKQNADIDEAQIEIAMMRGDWNAATAAARRAESI
                     LSSDPARLSLLRLIVWPRLALTLALSGRFTEAESIIAKTPPDCDFCLRMRGNIAAAKH
                     DWAGAARMFSIVSARAPHIPFADTDWGAMLLRKGDFDGAIAKFESAHRKGPHFADPLE
                     MWGEALMAKNRSDRALAKFEEANKYAPNWGRLHLKWGEALYYAGKHDEAKKQFALAFA
                     LDLSAPEKSELLKISRRT"
     gene            4243..6174
                     /locus_tag="VG274_03945"
     CDS             4243..6174
                     /locus_tag="VG274_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05834.1"
                     /translation="MNEQQENSTDRGPAAWTALGAASREKADAFLAEQTVLARLQADD
                     LRREDRLRHWSLRVRHISDVMKLTFEVTAALIALAVIVIIAAAIWSAAHDDSVVIDAF
                     RTPADMAQRGMSGDVVASQLLDRLAQLQAQTDSSRAPGTYASDWGSEIKVEIPNTGVS
                     IGEAYRYLASWLGHQTHISGEVWRTETGISLAVRTTGTSTVEFEGRERDLGALLKRAA
                     ESVYGQTQPYRYAIYLASHGRNAEEEKILRGLALNGPTSEKPWAYTVWAYSALIADNV
                     PEALRRSRKAVELEPDLPLAQNNLAFFEANVGHDEQELRASQTTRRALDGNGGHLIIS
                     RAATAVGIETDANIAEELGDFRGAVAQYDRLAGVADFEGSQWLSGRMGAADAAIDHDV
                     AGSRRLLGTGRDANLVASSITGFGWQLPNFRFAQFEQFVAVEDWKSARTDIEAALATP
                     AAGTRAGRAFLRAVAWPWLALAEAKTGDLNAAWREIGGTPLDWYLCLRVRGQIDSLQN
                     NWNGAAYWFARAAAVAPSPPFAYADWAATLLRKGDADAAIAMLKVAHERGPHFADPLE
                     MWGEALMAKNRSDFALAKFGEANKYAPSWGRLHLKWGEALFYTDMKDEAQKQFVIASH
                     LDLSQGDSASLSQWMAHHG"
     gene            6167..>8033
                     /locus_tag="VG274_03950"
     CDS             6167..>8033
                     /locus_tag="VG274_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05835.1"
                     /translation="MAEEEARESTSSANPSDPVAWAALSVASRVKADAFLDDQSALVR
                     LQAKELAHELNLRHWSLRVRHLSDILRLTFEFGVALIILLLVALCVAAVWNASRADGI
                     VVDTFSVPPRFAQAGMAGDVIADDLTNRVAAVRDFANAHSIAHSKGVRNERDEEIKVE
                     IPDTGVSLAEVSRYLRSWLGHERHLGGNVRIRDDGKIALTTALDGTNATTFVGSAGDL
                     DRFEQQAAEHVFQGVDPSNYVLYLYGKKRPAEAEMAIQHLVRVADSPGMLSDGYSLWG
                     NWTRNYTADLSLALKRCRIAADTDPKAVPPHMEMMAVFEDMGHDEEALREAREIPGFR
                     QEEQYAWREGPGFGQVVEIALLEIHTTMGDFGHAEIDACGWCSRPEMFLGRAEYAARM
                     HDIAHSRALIAAAGSMDQLDPALLNRATWFADAAAGDWQKAVADARGYGATATSTNPR
                     LQAIDLRTGITPLLAVALAHAGKFTQAHAEIDSTPGDCVLCERARGEIDGLENNFGGA
                     AWRFARAARDAPSIPFATADWGAMLVRKGAYDAAIAKFQQANKIGPHFADPLEMWGEA
                     LMLKSRSDLALAKFEEANKYAPSWGRLHLKWGEALMYVGKKEEARNQLAIATHFDL"
BASE COUNT         1587 a         2446 c         2564 g         1436 t
ORIGIN      
        1 aggggccatc gcttccgttc gcttatacgg actggggcga gatgctgctc gcgaaaggtg
       61 attacaacgc ggcaatcgat aaattccgcg acgccgatct caaaggtccg cacttcgccg
      121 atccgctgga gatgtggggc gaggcactga tggcgaaaaa ccgctccgat ctcgcgctgg
      181 cgaagttcga ggaagcaaac aaatacgcgc ccaactgggg ccgcctgcat ctgaaatggg
      241 gcgaggcact gatgtatgtg ggcaaacgcg acgaagcaaa ggatcagttc gcggctgctg
      301 ggcgactcga tctgtcgtca accgacaccg ccgaactcgc ccgcgtgaag gcaccgcatg
      361 gctgaggatt tcgagagcga ggaagcagga attcgcccgg ccggacgcgc ttccgactcg
      421 cttgccgccc atctcgcaat aaaaacagcc ggagaatcgt cagaagaagc gcgcgaattc
      481 ctgcgcagcc agtgtcgcct tgccgacctg cagacggaga atctgcgcct ccagaacgag
      541 aacctgggaa agcaggacga atacgaattg tctcatctca ggtggcggcg cttcaacgat
      601 cagatgcgcg gcgcgatgca catggtgctg gcggcgcttg gcgccctggc cgtaatcggc
      661 atctgcgctg cggtgtggaa cgcgagccgc gcacagggtt ttgtcatcga ggcattctcg
      721 gttccgcccg gctatgcgca acgcggcatg gcgggcgacg tcgtcgccgg cgacatcacc
      781 gacaaaatcg cgtcgatccg tcagttcgcg aatctgaatt ccttcagccg gtcgaaggaa
      841 gtgagcgagg accgcaagga aaatgttaga gtggaaattc ccgagacggc aatctcggtc
      901 gccgaagcct ggcgctatct gcgcaactgg ctcggcgacg agcgccatct cacgggaagc
      961 ctgcgcgaca cgggcgcagg acaggcgact ctcacgctca gtgcgccggg tgcagccaac
     1021 gtatccgtct cgggcgctgc ggccgatctc gctcggctgg aggcgcaggc ggccgaacag
     1081 atgttcggcc gtatcgatcc cgtcaactat gtgaattatc tcaccgccaa agggaggcat
     1141 cgcgaagcgc ttgccgcagc gaagcactac gcctccattg ccgtaggcat tcccgagcgt
     1201 agcgattcct ttgccctgtg gagttatacg acgttcgagg cgacgggcga ttttgcactc
     1261 gcgatccagc gcgcaaggat cggcatcggc atcgatccga agctggctgc gacgcgcgtg
     1321 cagttgatga acgaatattt ctatctcggc cggtatgaag acgcgctgcg cgaggcacgc
     1381 cctgtcctca ccctgaacga atcggaccag ctggctgcgc atcgcagcgg cgggtttgca
     1441 gccatgctcg ttctgtccgc cgggcgcgcg gcctatatgc tcggagattt cgccgacgcg
     1501 gccagctcaa gatgcggact cggttgcgcc tatccggagc aagcgctctc tcgcgccaca
     1561 aacctcgcgc gcgagcatga cgtttcgaac agtcgcgatc tgatcgccag tgcgctggcg
     1621 gcgggcgcca gcgacggcgg cgcccttggt caggcccgtt actacgcgga tgccgcggcg
     1681 gacgactggc aggctgccgt cgcggaggcc aaggcagccg cagccgccta cgcggaacca
     1741 aagggcaatc tcagcgggaa gtatattgcg accgtggaag ccacacgttt cgcgccaccg
     1801 ctggcggttg ccgaggcgca taccggcgat ttcgcaacag cgcacagcgt cgtcgacgcg
     1861 acaccggccg attgctacga gtgcgctctc gcccgcggca acatcgatgc catcgagaaa
     1921 caatcgggcg ccgcttccag ttggttcgcg cgcacaattg ccctgggtcc ctcgatcccc
     1981 ttcgcttacg ccgattgggg ggagatgctg cttgcggatg gcaactacga cgcagccatc
     2041 gcgaagttca ggcttgcaaa ccagaaaggt ccgcacttcg ccgatccgct ggaaatgtgg
     2101 ggcgaggcgc tgatggcgaa gaaccgctcc gacctcgcgc tggcgaaatt cgaggaagca
     2161 aacaaatatg cgcccaactg gggccgcctg catctcaaat ggggcgaggc gctgttttac
     2221 gcagggcaaa agaccgaagc ggaaaagcaa ttcgcagtgg cgtcgcaact tgatctctca
     2281 cagccggaca aggctgtgct tgcgaaattg gtgaaccatg gctgaggaac tcgtgcgcga
     2341 atccgagcat tcgccccggc agcggtcgga tgctgtggca tggaacatcc ttggcgaagc
     2401 cagccgcgag aaggctgacc aattcctcga tcgccagggc gcgctatccc agctgcaaat
     2461 ggaaaatctg cagctggaga acaagaggct caatgcgcag cacgcgttcg aggtctcaca
     2521 tctgcgcttc cggcgtttca gcgactacgc acgtgcggcg ctcgagggtg cggggtttct
     2581 cgtcattctc ctcctcgtct gcggactcgg cacgatggtg tggagcgcaa cacaggatca
     2641 cgacctcgtg gtggacgcat tcctggtttc tcccgacgtc gcgcaatccg gcatgacggg
     2701 cagcgttctg gcaggccgcg tgctcgatgc cttcggccgg atgcaatcgt cgatcggaat
     2761 ctccgttgtc cagggcgcgg gctcctatca cgccggatat tccgaagagg ttcgcgtgga
     2821 aatcccgcaa accggaattt cgatcggaga gctcaatcgc tatctgcgcg aatggctcgg
     2881 tcacgaaacc catgtcaccg gagatctggt acacgccgct aagggctatg cgcttaccac
     2941 ccgtgcgagt ggtcaaccgg gtgtcaccat cgaaagcgcg gatatggacg cactggtccg
     3001 aaaggcagcc gagcacatgt tcgcggtcgt tcgtcctctg cgttatctcg attatctcga
     3061 ggcgaacaat cgcgtccgcg aagctcttgc agcggtcgtt cccctgacca aagccggcga
     3121 tgcgcatgat cgtgcggttg cctacgcggc atgggcaggc ctgttgtcgg actcccacga
     3181 tcccgattcc gcgctcgcca aggcccgcat ggcagcctcc atcgatccgc aaaacccgac
     3241 tgcgatgggc tggctcgcga gcatcgaacg gaccctgggt cacgacgaag cggcctggag
     3301 tctctcttcg cgcaacatag gtctctggca cggagcggag gttgcagatc tcgatcccgg
     3361 gcttgtcgcg actgctcccg ccttcttcac gtacagaagg gacgcgtcca tcggagattg
     3421 ggcggcggtc ctgacggcgg aagccgattt cgcggacggc agcggatatt ccacgcctgc
     3481 caatcgcgcc ttcgaagcag cccacgatca cgatgtagcg ttggcgcttt cctatgtgaa
     3541 atccatcccg ctaagggcca attcggtaaa gcagaacgcc gatattgatg aggcgcagat
     3601 cgaaatcgcg atgatgcggg gcgactggaa tgcggcgacc gctgccgcgc ggcgggcgga
     3661 atcgattctc tcttccgatc cggcgcgact gagcctgctg cgcctcatcg tctggccgag
     3721 actggcgctc acgctcgcac tctcggggcg cttcacagaa gccgaaagca tcatcgccaa
     3781 gacgccgccc gattgcgatt tctgtttgcg gatgcgcgga aacatcgccg cggcaaagca
     3841 cgactgggcc ggtgccgcgc gcatgttttc gatcgtctcc gcgcgcgctc cgcatatccc
     3901 cttcgccgat accgattggg gcgcgatgct tctgcgcaag ggcgattttg atggggcgat
     3961 tgcgaaattc gagtctgcgc atcggaaggg gccgcacttc gccgatccgc tggaaatgtg
     4021 gggcgaggcg ctgatggcga agaaccgctc cgatcgcgcg ctggcgaaat tcgaggaagc
     4081 aaacaaatac gcgcccaact ggggccgcct gcatctcaaa tggggcgagg cgctgtacta
     4141 cgcaggcaag cacgacgaag cgaagaagca attcgcgctg gcatttgcgc tcgatctctc
     4201 agccccggaa aaatccgaac tattgaaaat ctcgcgccgg acatgaacga gcaacaagaa
     4261 aattccaccg atcgcggacc ggctgcctgg acggcactcg gcgccgccag ccgcgaaaag
     4321 gccgatgctt tcctcgccga acaaaccgtg ctggcgcgat tgcaggcgga cgacctgcgc
     4381 cgcgaagaca ggcttcgcca ctggtcgctg cgcgtgcgtc acatcagcga cgtgatgaag
     4441 ttgacgtttg aagtgacggc cgcgctgatc gcgctcgcgg tcatcgtcat catcgcggcg
     4501 gcgatctggt ctgcggccca tgacgacagt gtcgtgatcg atgcgttccg gacgccggcc
     4561 gacatggcgc aacgcggaat gagcggcgac gtcgttgcaa gccagctgct cgaccggctt
     4621 gcgcagctgc aggcgcagac cgattccagc cgtgcgcccg gtacttacgc cagcgactgg
     4681 ggcagcgaga tcaaggtcga gattcccaat accggcgtct ccatcggcga agcctatcgc
     4741 tatctcgcaa gctggctcgg ccatcagacg catatttcgg gcgaagtctg gcgcacggaa
     4801 acaggtattt cgctcgcggt tcgcacaacc ggcacgtcca ctgtggagtt cgaggggcgg
     4861 gagcgcgatc tgggcgcgct tctcaagcgc gcggcggaaa gcgtttatgg ccagacgcag
     4921 ccataccgct acgcgatcta tctggccagt catggacgca acgcggagga ggagaaaatc
     4981 ctgcgcggtc tcgcactcaa tggtcccact tccgaaaaac cctgggccta cacggtgtgg
     5041 gcctattccg cgctgatcgc ggacaacgtg ccggaggcgc ttcggcgttc gcgcaaggcc
     5101 gtcgaactcg aacccgatct tccgctcgcg cagaacaatc ttgcattttt cgaggccaat
     5161 gtcggacacg atgaacagga gctccgggcc tcccagacca cgcggcgggc gctggacggg
     5221 aacggcgggc atctcatcat ttcccgcgcc gccaccgccg tcggaatcga gaccgatgca
     5281 aacattgccg aagagctcgg agattttcgc ggcgccgtcg cgcaatatga caggctcgcc
     5341 ggcgttgcgg atttcgaggg ctcgcaatgg ctgtcgggcc gcatgggcgc ggcggacgcg
     5401 gcaatcgatc acgacgtcgc cgggtcgcgc cggctgttgg ggaccggtcg cgacgcgaat
     5461 ctcgtcgcca gttccatcac cggtttcggc tggcagctgc cgaatttccg tttcgcgcag
     5521 ttcgagcagt tcgtggctgt cgaggattgg aaatcagcgc gcacggatat cgaagcagcc
     5581 ctggcgacgc cggcggccgg gacacgtgca ggacgcgcgt tcctgcgcgc agtggcctgg
     5641 ccgtggctgg cgcttgcaga agcaaaaacc ggcgacctga acgccgcttg gcgcgaaatt
     5701 ggcgggacgc cgcttgattg gtatctctgc ctgcgcgtgc gcggacagat cgactcgctt
     5761 cagaataact ggaacggcgc ggcgtactgg ttcgcacgtg cagccgcggt agcgccctcc
     5821 cctccctttg cctatgcgga ttgggcggcg acgttgctac gcaaaggcga cgcggatgct
     5881 gcaatcgcca tgctaaaggt cgcgcacgag cgcggcccgc acttcgccga tccgctggag
     5941 atgtggggcg aggcactgat ggcgaagaac cgctccgact tcgcgctggc gaaattcggg
     6001 gaagccaaca aatacgcgcc cagctgggga cggctgcatc tcaaatgggg tgaagcgttg
     6061 ttctacacgg acatgaagga cgaagcgcag aaacaattcg tcatcgcttc gcatctcgat
     6121 ctctcacaag gcgacagcgc gtccctctcg cagtggatgg cgcaccatgg ctgaagaaga
     6181 agcccgcgaa tccacctcgt cggcgaatcc ttccgatcct gttgcctggg cagcgctcag
     6241 tgttgcgagc cgcgtgaagg ccgatgcgtt tctggacgat cagtcggccc tcgtgcgtct
     6301 tcaggcgaag gagctcgcgc atgaattgaa tctgcgccat tggtcgctgc gcgtgcggca
     6361 tttgagcgac atactcaggc tcaccttcga gttcggcgtg gccctcatca tcctgcttct
     6421 cgtcgcgctt tgcgtggccg cggtatggaa cgcgagccgc gccgacggca tcgtcgtcga
     6481 caccttctcg gtgcctccgc gattcgccca agcgggaatg gcgggcgatg tgatcgctga
     6541 cgacctgacg aaccgggtcg cagccgtgcg cgacttcgcg aacgcgcatt cgatcgcaca
     6601 ctccaagggt gtgcggaacg aacgcgacga agagatcaag gtcgaaattc ccgataccgg
     6661 cgtctcgctc gcggaggttt cgcgctatct gcgatcatgg ttgggacacg aacggcatct
     6721 gggcggcaat gtgcgcatca gagatgacgg gaaaatagcg ctcaccactg cactggacgg
     6781 caccaatgca acaacctttg ttggttccgc cggcgatctt gaccgtttcg aacagcaggc
     6841 ggcggagcat gtgttccagg gcgtcgatcc ctcgaactac gtactctatc tctatggaaa
     6901 aaaacgcccg gcggaagctg agatggccat tcagcatctc gtccgcgtag ccgacagtcc
     6961 gggaatgctt tcggatggat attccctgtg gggcaactgg acaaggaatt acaccgccga
     7021 tttgtcgctc gcactgaagc gctgtcgaat agcggcggat accgatccaa aggcggtccc
     7081 gccgcatatg gagatgatgg ccgtgtttga ggatatgggg cacgacgagg aggcgttgcg
     7141 agaggcccgg gaaattccag gtttcaggca ggaggaacaa tatgcctggc gagagggccc
     7201 cggcttcgga caggttgtcg aaatcgcgct cctggaaatc cacaccacga tgggtgattt
     7261 cggccatgcg gagatcgatg cctgcggatg gtgcagccgc cccgagatgt ttctcggccg
     7321 ggcggaatat gccgcgcgca tgcacgatat cgcgcatagc cgcgcgctga ttgcagcggc
     7381 cggttcgatg gaccagctgg atcccgcgct cctcaacaga gcaacctggt ttgcggacgc
     7441 tgcagccggc gactggcaga aggcagtcgc cgacgcgcgt ggctacggcg cgactgcgac
     7501 cagcacgaat ccgcgtctgc aggcgatcga cctgcgcacc gggatcactc cattgcttgc
     7561 ggttgctctg gcgcacgcgg gaaaattcac acaggcgcac gccgagatcg acagtactcc
     7621 cggtgactgc gtgctttgcg aaagggcgcg cggcgaaatc gacgggctgg aaaacaattt
     7681 tggtggcgca gcctggcggt ttgcgcgcgc cgcccgcgac gcgccctcaa ttccgttcgc
     7741 cactgcagac tggggcgcga tgctggtgcg caagggcgcc tacgatgcag cgatcgcgaa
     7801 gtttcaacaa gcgaacaaga tcggcccgca ttttgccgac ccgctggaga tgtggggcga
     7861 agcgctgatg ctgaagagcc gctccgacct tgcattggcg aaattcgaag aagctaacaa
     7921 atacgcgccc agctggggcc ggctgcatct gaaatggggc gaggcgctga tgtacgtggg
     7981 caagaaagaa gaagcgcgaa atcagcttgc catcgctacg catttcgatc tgt
//
LOCUS       DASZMC010000202         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1471898,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000202 DASZMC010000000
VERSION     DASZMC010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471898"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VG274_03955"
     CDS             <1..372
                     /locus_tag="VG274_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase domain-containing
                     protein"
                     /protein_id="HWE05836.1"
                     /translation="RLLSRMGDIYLAPLFQQLLVQIDAKMRNAAHVRSAFDDLEAVLG
                     TMTNFVEGPDYAIGERLSLADCALAPFFFFIDIFLARTFDLPSPLRGSMKLYYDNSQK
                     DPHIARAIREMNAALDSRLAR"
     gene            complement(401..1492)
                     /locus_tag="VG274_03960"
     CDS             complement(401..1492)
                     /locus_tag="VG274_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWE05837.1"
                     /translation="MRAADDRQEKFSGTKEVAEAYRFDENRLAQYLCARIEGFATPLR
                     VRQFKGGQSNPTYQLVTPNRTCVLRRKPPGKLLPSAHAVEREYRIIAALHPTGFPVAR
                     PYVLCEDESVIGTPFYVMEYVEGRVFWGPLLPRLAPEQRSSIYDAMNATLARLHMLDY
                     EKLGLADFGKPGNYIARQIARWTKQYVASQTESIAEMEKLIDWLPEHLPLEERSCIVH
                     GDFRLDNMILHPDRSEILVVLDWELSTIGDPLADFTYHLMQWQMPPDIATSGTSSLLD
                     VDLASLDIPTMDAYVEMYCRRTGRKTTPNMNYYAAYNFFRLAAILQGIVGRVRDGTAA
                     NENAAQNAAGVRPLAERGWHFAKLAGATD"
     gene            complement(1489..2754)
                     /gene="purD"
                     /locus_tag="VG274_03965"
     CDS             complement(1489..2754)
                     /gene="purD"
                     /locus_tag="VG274_03965"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWE05838.1"
                     /translation="MKFLLIGSGGREHALAWALAASPIVSKLYCAPGNAGIAEVAECV
                     PVPVNDLDGLVAFAKAKGIDFVVIGPEAPLVAGLWDRLEAAGIKALGPSARGAMLEGS
                     KGFLKELCRENAIPTAGFARFHERDTALAYAAGHRHPLVIKADGLAQGKGVIVAETGA
                     ESSAAISEIWSNYGPGLVIEEFLDGEEASLFALTDGEHIIPLAGAEDHKRAFDGDRGP
                     NTGGMGAVSPSSLLAPQVIKKTLSQIIKPTIAAMAARGTPYMGVLYAGLMIRDGEPSL
                     VEYNCRFGDPECQVLMMRLKSDLATALLAARDGEIAHLDLRWKDDAALAVVMAACGYP
                     GNYSRGSEIRGVQDAAKIKGVQIFHAATEKRDGKLCATGGRVLNVAALGSNVEEARSR
                     AYEAVHRIDWPEGFFRSDIGTRGLARNAR"
     gene            2805..>3718
                     /gene="xseA"
                     /locus_tag="VG274_03970"
     CDS             2805..>3718
                     /gene="xseA"
                     /locus_tag="VG274_03970"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWE05839.1"
                     /translation="MSEPERRANLPEFSVSEIAAALKRTVEGAFPFVRVRGEISGLKF
                     HSSGHVYFDLKDDKAVLNAVVWRGTAQTLRLRPEQGLEVVCTGRVSTYAGSSRYQLIV
                     EQMELAGLGALMAMIEERKKRLAAEGLFAEARKKSLPFLPTIIGVITSPTGAVIRDIM
                     HRLDARFPSRVLLWPVAVQGERAAADVAAAIHGFNALAPGGVVPRPDLLIVARGGGSI
                     EDLVAFNEEIVVRAAAESAIPLISAVGHETDTTLIDLAADRRAPTPSAAAEMAVPVRT
                     ELLAQTLDLERRNLRCFTRGLDDRRRLL"
BASE COUNT          665 a         1168 c         1133 g          752 t
ORIGIN      
        1 cgtctgctct cgcgcatggg cgacatctat ctggcgcctc tgttccagca actgctcgtt
       61 caaatcgatg ccaagatgcg caacgcggcg catgtcagat cggcctttga cgaccttgag
      121 gccgttctgg gcacgatgac gaatttcgtc gagggccccg actacgccat cggcgaaaga
      181 ctgagcctcg ccgattgcgc cttggcccca ttcttctttt tcatcgacat ttttctggcg
      241 cgaacctttg acctgccgtc cccgctcagg ggctcgatga aactctacta cgacaattcg
      301 cagaaggacc cgcacatcgc cagagccatc cgcgagatga acgcggcgct cgattcgcgg
      361 ctggcacgtt gacgattgct gcaatcggcc tgtcggttgg tcagtccgtc gcgcccgcaa
      421 gctttgcgaa atgccagccg cgttcggcaa gcgggcggac tccggccgcg ttctgtgcgg
      481 cgttctcgtt cgcggcggtt ccgtcgcgca cccgcccgac aatgccctga aggatcgcgg
      541 ccagccggaa gaaattgtag gccgcgtaat aattcatgtt tggcgtggtt ttgcggcccg
      601 tccgccggca atacatctcc acataggcat ccatcgttgg aatatccaga cttgccagat
      661 cgacgtccag aagactgctt gtgccgctcg tcgcgatgtc gggcggcatc tgccattgca
      721 tcaggtggta ggtgaagtcg gcgagcggat cgccgatcgt cgacagctcc caatcaagca
      781 cgaccagtat ctcggatcgg tcaggatgca ggatcatgtt gtcgagacga aaatcgccgt
      841 gaacgatgca actgcgttct tcgagcggga ggtgttcggg cagccagtct attagcttct
      901 ccatttcggc tatcgattct gtttgcgagg cgacatactg cttggtccag cgtgcaatct
      961 ggcgggcgat ataatttccg ggctttccaa aatcggccag gccgagtttt tcgtaatcga
     1021 gcatgtgcag ccgcgcgagc gtcgcgttca tggcatcgta gatcgaagag cgttgctccg
     1081 gtgcaaggcg gggcagtagc gggccccaaa agacgcgtcc ctcaacatac tccatcacgt
     1141 agaaaggcgt gccgatgacc gactcgtcct cgcagagcac atagggtctg gcgaccggaa
     1201 agccggtcgg gtgaagcgcg gcaatgatgc gatactcgcg ttcgacggca tgcgcggacg
     1261 gcagcaattt tcccggcggt tttcgccgca ggacacaggt gcgattcggc gtgacaagct
     1321 ggtaggtggg attggactgt ccgcccttga actggcgtac gcgcagcggc gtcgcgaaac
     1381 cctcgatgcg cgcgcagaga tactgcgcaa gtctgttctc gtcgaagcga tacgcctcgg
     1441 ccacttcctt ggtgccggaa aatttctcct ggcggtcgtc cgctgctctc atcgcgcgtt
     1501 cctcgcgagc ccccgcgttc cgatgtcgct gcggaaaaat ccctccggcc agtcgatgcg
     1561 gtgcaccgct tcatacgcgc ggcttcgcgc ttcctccaca ttgctcccca gggcggcgac
     1621 attcaggacc cgcccgccgg tggcacaaag ctttccgtcg cgcttttccg tcgctgcgtg
     1681 aaatatctgc acgcctttga ttttcgcggc atcctgcacg ccacggattt ctgaacctct
     1741 tgagtaattg ccgggatatc cgcacgcggc catcaccacc gcgagcgcag catcgtcttt
     1801 ccaacgcaga tcgagatggg cgatttcgcc gtcacgcgcc gcgaggagcg cggtcgcaag
     1861 gtccgatttg aggcgcatca tcaatacctg gcattcggga tcgccaaatc tgcagttgta
     1921 ttcaaccagg ctgggctctc cgtctctgat catcaatccc gcgtagagaa cgcccatata
     1981 gggcgtgccg cgcgccgcca tggccgcaat cgtcggcttg atgatctgcg acaatgtttt
     2041 cttgatgacc tgcggcgcca gcagcgacga cgggctcacg gctcccatgc cgccggtgtt
     2101 gggaccccgg tcgccatcga aggcgcgctt atggtcctcg gcgcccgcaa gcgggatgat
     2161 gtgctcgccg tccgtcagag cgaagaggct cgcttcctcg ccgtcgagaa attcttcgat
     2221 gacgagtcct gggccgtaat tcgaccaaat ctcggaaatt gccgcggatg attcagcacc
     2281 tgtctccgcg acgatgacgc ctttgccctg cgccaggccg tccgctttga tgaccagcgg
     2341 atggcggtga ccggcggcgt aggcgagggc ggtgtctcgc tcgtgaaatc gcgcgaaccc
     2401 ggcggtcggt atggcgttct cgcgacagag ttctttcaga aaacctttcg agccctcaag
     2461 catcgcccct cgcgcgctcg gacccagggc tttgatgccg gcggcttcga gccggtccca
     2521 gagtccggct accagcggcg cctcgggccc gatgacgacg aaatcgattc cctttgcctt
     2581 ggcgaacgcg accaaaccgt caagatcgtt gacgggaaca gggacgcatt ctgccacctc
     2641 tgcgatgccc gcatttcccg gcgcgcaata gagcttcgag acgatcggac tcgctgccaa
     2701 cgcccatgcc agcgcatgtt cgcgcccgcc cgaaccaatg agaagaaatt tcacggcact
     2761 ccgccaattc aatcctggcc gcatctccta tatcattccg attcatgtcg gaaccggagc
     2821 gacgggcaaa tctgcccgaa ttcagcgtca gtgagatcgc agccgcgctc aagcggacgg
     2881 ttgagggcgc gtttccgttc gtgcgggtgc ggggcgagat cagcggcctc aaattccatt
     2941 cctcgggaca cgtttatttc gacctcaagg acgacaaggc ggtgctcaac gccgtcgtct
     3001 ggcgcgggac ggcccagacc ctgagattga ggccggagca ggggttggaa gtcgtttgca
     3061 ccgggcgggt ttcaacctat gccggctcgt cgcgctacca gctcatcgtc gagcagatgg
     3121 aactcgccgg gctcggcgcc ttgatggcga tgatcgagga gcgaaagaag cggctggctg
     3181 cggagggcct gtttgccgag gcgcgaaaga agagccttcc gttcctgcca acaataatcg
     3241 gggtcatcac ctcgccgacc ggcgccgtta ttcgcgacat catgcaccgt ctcgacgccc
     3301 gttttccgag ccgggtgctt ttgtggcccg tcgccgtgca gggcgagcgc gcggccgctg
     3361 acgtcgctgc ggcaattcac ggcttcaatg cacttgcgcc cggaggcgtt gttccgagac
     3421 ccgatcttct gattgtcgcg cgcggcggcg gctccatcga ggacctcgtg gcgttcaatg
     3481 aggagatcgt ggtgcgggcg gcggcggaga gcgccatccc gttgatctcg gctgtcggcc
     3541 acgaaacgga cacgacgctc atcgacctgg ctgccgaccg tcgcgctccc acgccgagcg
     3601 cggcggccga gatggcagtg cccgtgcgca ccgagcttct tgcccagacg ctggatctgg
     3661 agaggcgcaa tctcagatgc ttcacacgcg gattggacga ccggcgcagg cttttgca
//
LOCUS       DASZMC010000203         1864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1472805,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000203 DASZMC010000000
VERSION     DASZMC010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472805"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="tilS"
                     /locus_tag="VG274_03975"
     CDS             complement(<1..1080)
                     /gene="tilS"
                     /locus_tag="VG274_03975"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWE05840.1"
                     /translation="MTKNRRLALTRSLMAAGERVCVAVSGGADSVALLLALVEANRAK
                     EPLGVVLSAVHVHHGLRGADADADEAFVRDLCEQLGAPLIVERVDVAGRQAAEREGLE
                     EAARELRYQAFRKLIKSGKADLVATAHTRDDQAETVVMKLLRGAWTEGIAGIAPVVES
                     DGDATRGRIVRPLLNTRRAEVEAYLHQRGQTWREDATNRDVSLTRNRVRHELMPMLRQ
                     FNPAIDLALARLAEIARDEEAFWQAEVARVLPQVLLPGRPVRGGGRAVSTVTGSSSCA
                     VEVERLRSMSPALRRRVLRTAVRSLGRRISADETAKLLALAGLAEVEPPVAARPGVRL
                     ELAGGLRAERTAREIQLRVDAAPFVA"
     gene            1052..>1864
                     /locus_tag="VG274_03980"
     CDS             1052..>1864
                     /locus_tag="VG274_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HWE05841.1"
                     /translation="MSASLLFFVIVSTFERFHLAFERFLSARYHHLTEPLLTMNNTYA
                     RRFRPAFKSSLKSSVLTIALFSLTAACRAGAAQDVHVKVDPHGSGSTASTTDYTSIQQ
                     ALDHAPQAAAPYRTIIEIVPGTYHERINVTRNRPRITLLGLGKSPEDVVITYSMNAKQ
                     AGGTFFTETAQINGDQFEADKVTFENSAGPTGQAVAAAVRSDRSIFKHCRFLGDQDTL
                     FADYGRQYYVDSFIAGGVDFIFGNATAVFDHDTISEIRNGQITAQSRTSPEQT"
BASE COUNT          340 a          700 c          482 g          342 t
ORIGIN      
        1 tgcaacgaaa ggagcagcat caacccgcag ctgaatctct cttgccgtgc gctccgcccg
       61 cagccctcca gccagctcca ggcgcactcc ggggcgcgcc gccacgggtg gctctacctc
      121 cgccaaaccc gccagcgcca gcaacttggc cgtctcatcg gcggagattc tgcggcccag
      181 ggagcgcacg gctgtacgta gcactcgcct gcgaagcgcc ggagacatcg agcgcagcct
      241 ttcgacctcc acggcacagc tgctgctgcc agtaacggta ctcacggcac gtcctccgcc
      301 ccgcaccggc cggccgggga gcagaacctg cggcagcacc cgcgccacct ctgcctgcca
      361 gaatgcctcc tcgtctcttg caatctccgc cagccgagct aacgcgagat cgattgccgg
      421 gttgaactgc ctgagcattg gcatcaactc atgtcgcacg cgatttctcg tgagcgacac
      481 atcgcggttc gtcgcatcct ctcgccaggt ttgaccacgc tgatgtagat acgcctctac
      541 ttccgcccgt ctggtgttga gcagcggccg cacaatccgc cctcgcgttg catctccatc
      601 gctctcaacg acaggggcga tcccggcgat cccctctgtc cacgcgccac gcagcagctt
      661 cattacgact gtctcggcct gatcatcgcg cgtgtgcgcc gtcgcgacga ggtcggcctt
      721 tcccgacttg atcagcttgc ggaacgcctg atatcgcagc tcacgcgcgg cttcttctaa
      781 tccctcgcgc tcagccgcct gcctgcccgc gacatctacg cgctccacga tcagcggcgc
      841 gcctaactgc tcgcaaagat cacggacaaa cgcctcatct gcatcggcat cggcgccacg
      901 cagaccgtgg tgcacgtgca ccgcgcttag caccacgccc aacggctcct tcgcgcggtt
      961 cgcctctacc agcgccagca gcagcgccac agaatcggcc ccgcccgaca ccgccacgca
     1021 gacccgctcg cccgcggcca tcagcgagcg agtgagcgcg agtcttctgt tcttcgtcat
     1081 cgtctctacc ttcgagcgct ttcacttggc cttcgagcgc tttctctcgg cgcgttacca
     1141 tcaccttaca gagcctttac tcacgatgaa caatacctac gctcgtcgat tccgccccgc
     1201 cttcaagtct tcgcttaaat cttcggttct caccattgcg ctcttctcat taacagccgc
     1261 gtgccgggca ggcgcagcgc aggacgtcca tgtcaaagtc gacccgcacg gttcgggctc
     1321 gaccgccagc accaccgatt acacctccat ccagcaggcg ctcgaccatg cgccacaggc
     1381 tgccgcaccg tatcgcacca tcatcgagat cgttcccggc acctatcatg agcgcatcaa
     1441 cgtcacgcgc aaccgcccgc gcatcacgct gctggggctt ggcaaatcgc ctgaggatgt
     1501 cgtgattacc tactcgatga atgcgaagca agccggcggc accttcttca ccgaaaccgc
     1561 acagatcaac ggcgatcagt tcgaggcgga caaggtgacg ttcgagaatt ctgccggtcc
     1621 cacgggccag gccgttgcgg ccgccgtgcg ctccgatcgc tccatcttta agcactgccg
     1681 ctttctcggc gaccaggaca cactcttcgc cgactatgga cggcaatact acgtggactc
     1741 attcatcgca ggcggcgtcg acttcatctt cggtaacgca accgctgtct tcgaccacga
     1801 caccatcagc gagatccgca acggacagat caccgcgcag tcgcgcacct cgcccgagca
     1861 gacg
//
LOCUS       DASZMC010000204         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3389527,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000204 DASZMC010000000
VERSION     DASZMC010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3389527"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..781
                     /locus_tag="VG274_03985"
     CDS             71..781
                     /locus_tag="VG274_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWE05842.1"
                     /translation="MIDQHDLPMNPRLLGKPGVGKTTLAYAAAERLRREVYIMQATVD
                     TRPEDLLVTPVIEGEGRLRYVASPLVTAMLRGGIVIVDEGNRMSEKSWASLAPLLDGR
                     RYVESIVAGIKIKAHPLFRLVATMNDDASTFDLPEYIHSRLQPQILIDFPERHEEYAI
                     LKENLPFGDDYVLNYVTDFLQQAHAADERYTARDGINIGRYAMKLAQQNSGAARDMLR
                     DAIVQTLGEEALRYVPRS"
     gene            765..>2376
                     /locus_tag="VG274_03990"
     CDS             765..>2376
                     /locus_tag="VG274_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05843.1"
                     /translation="MSLEAEIKSGALERGRIRYFPVVPGRLEFTIELRRRLLADKPAI
                     VAVELPGFLESTYRRALARLPEMSVMLYHRDGTRASSKESSDDEDQAIFIPVEPTDPF
                     VEAVRTAIEIEAQIIFLEPDTAERPHLHSAYPDTYAIRKIGIDKYVEAYRVYPQMRTD
                     EVTAHASAMAWKLQGADPLAGMFVVTALNMLDPLLDAMESPQPAPSRGKRYDIDLLNP
                     HPDCLAEITVEYPYLQERYEFFRLDMKGEEALDRPKVQHDLLREAEGKYQTSTGDKLV
                     HWQRRMMARYTRNLAHISGDLTCSVYDIAVAARSVVDDNYGWEVWSMANRYLAQQEVS
                     ELDTVRLSASEIWINTKKLRIRRRLPRLKQRMKPTGLKPRKREATPGEWARQTTGDAI
                     CSYPPEDLVIEEYGRFVKQKAKAILSEERVRVEPFTTSILDGVDIRETIRNWHQRKIF
                     VRKADRLAGEVGAVVIIFDEDHEDRYTYLTTWLGEHQNESDMAFYSTAPFDHMIGPGI
                     GRAEYGGFLMSLPARRMYDVWADPDYDFA"
BASE COUNT          496 a          751 c          689 g          440 t
ORIGIN      
        1 ccccgaccag ctgaatatta catgggtcgg tcaggacgaa gtgatgaggc agctgctggc
       61 cgcctggctc gtgatcgatc agcacgacct cccgatgaac cctcgcctgc ttggtaaacc
      121 gggagttggt aaaacaacgc tcgcatacgc tgccgccgag cggctgaggc gcgaagtcta
      181 catcatgcag gccaccgtcg acacgcgtcc cgaagactta ctggtcacgc ccgtgattga
      241 aggcgaagga cgcctgcgtt acgttgcgtc gcccttagta accgcgatgt tgcgcggtgg
      301 catcgtgatt gtggacgaag gcaatcgcat gagcgaaaaa agctgggcga gccttgcccc
      361 tctgctcgac gggcgccgct acgtggagtc catcgtcgcc ggcatcaaga tcaaagcgca
      421 tccgttgttc cgcctggtgg cgaccatgaa cgacgacgcc agcaccttcg atctgcccga
      481 atacattcac tcgcgcctgc agccgcagat cctcatcgat tttccggagc gccacgaaga
      541 gtacgcgatt ctcaaagaga acctgccctt cggcgatgac tacgtgctga actacgttac
      601 ggattttttg caacaggcgc acgcggccga tgagcgttac acggcgcgcg acggcatcaa
      661 catcggccgc tacgccatga agttggcgca gcaaaactcc ggcgccgcgc gcgacatgct
      721 gcgcgatgcc attgtgcaaa cgctgggcga agaagcgttg cgctatgtcc ctcgaagctg
      781 aaatcaaatc cggcgcgttg gagcggggcc gcatccgcta tttcccggtc gtccccggac
      841 gcctggaatt caccatcgaa ctgcgccgcc gcttgcttgc cgataagccc gcgatcgtcg
      901 cggtggagct gcccggcttc cttgaatcca cttatcgccg cgcgctcgcg cgcctgcctg
      961 agatgtccgt gatgctttat catcgcgacg gtacgcgcgc gtcctcgaaa gaatcatccg
     1021 acgacgaaga tcaagccatc ttcattcccg ttgagcccac cgatccgttc gtcgaagccg
     1081 tgcgcaccgc catcgaaatc gaggcgcaga tcatttttct ggagcccgat accgccgagc
     1141 ggccgcatct gcacagcgcc tatcccgata cctacgcgat ccgcaaaatc ggcatcgaca
     1201 aatacgtcga ggcctatcgc gtgtatccgc aaatgcgaac cgacgaagta acggcgcacg
     1261 cctcggcaat ggcctggaag ctgcagggcg ccgatccgct ggccggtatg ttcgtggtca
     1321 ccgcgcttaa tatgttggat ccgctgctgg acgcaatgga gagtccgcag cccgcgccgt
     1381 ctcgcggcaa gcgttacgat atcgatctcc tcaatccgca tcccgattgc ctcgcagaga
     1441 tcacggtcga atatccctat ttgcaggagc gctacgaatt cttccgtctc gatatgaaag
     1501 gcgaagaggc gctcgaccga cccaaggtgc agcacgattt attgcgtgaa gccgaaggca
     1561 agtatcaaac ctccacgggc gacaagctgg tgcactggca gcgccgcatg atggcccgct
     1621 acacgcgcaa cctcgcgcat atcagcggcg acctgacgtg cagcgtgtac gatatcgcgg
     1681 tggccgcgcg ttccgtcgtc gacgataact acggctggga agtgtggtcg atggcgaatc
     1741 gctacctcgc gcaacaggag gtgtcggagc tcgacacggt gcgcctcagc gcgagtgaga
     1801 tttggatcaa cacgaagaag ctgcgcattc gccgccgcct tcctcgtctg aagcagcgca
     1861 tgaagcccac gggattgaag ccgcgcaaac gcgaagcgac tcccggcgag tgggcgcgcc
     1921 agaccacggg cgacgccatc tgctcctacc cgccggaaga tttggtgatc gaagaatacg
     1981 gccgcttcgt caagcaaaaa gccaaagcga ttctttccga ggagcgcgtg cgcgtggagc
     2041 cgtttactac ctcgatcctc gatggcgtcg atatccgcga aaccatccgc aactggcacc
     2101 agcgcaaaat ctttgtgcgc aaggccgacc ggctcgcggg agaagtgggc gccgtggtca
     2161 tcattttcga cgaggatcac gaagaccgct atacatacct caccacctgg ctgggcgaac
     2221 atcagaacga atccgacatg gcgttctatt ccacggcgcc gttcgatcat atgatcggcc
     2281 ccggcatcgg ccgcgccgaa tacggcggct ttctcatgag tctgcccgcg cgccgcatgt
     2341 atgacgtctg ggcagacccc gattacgatt tcgcgg
//
LOCUS       DASZMC010000205         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2005982,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000205 DASZMC010000000
VERSION     DASZMC010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005982"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VG274_03995"
     CDS             <1..286
                     /locus_tag="VG274_03995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE05844.1"
                     /translation="SRPKLAHTGDIGAMYVRDSARGTGLADALMTALLRHASTVVEQV
                     QLTVNAENTRAIKFYLRHGFRQIGIIPRSLHVGDRYYDDMLMFRCVSSGA"
     gene            complement(275..649)
                     /locus_tag="VG274_04000"
     CDS             complement(275..649)
                     /locus_tag="VG274_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05845.1"
                     /translation="MRVSFFTAGLFTIAALCAASAVAVIWRETTESGLPKPATITAYF
                     VAQCQMEKPECADMVTGAMRWDVNAKGLSRTCLAKRPSARTMARDTVIWLNAHTELHP
                     LPVEQGIAKAAEAIWPCKKSGA"
     gene            complement(654..1385)
                     /locus_tag="VG274_04005"
     CDS             complement(654..1385)
                     /locus_tag="VG274_04005"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002343.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HWE05846.1"
                     /translation="MTNQRDARVPQFFLTPGGACPYLPGRTERKVFARLTGALAQPLN
                     EALTHSGFRRSQSIAYRPACEGCSACVSVRIIADEMEFSRGQKRILKRNNDLVRTEVE
                     AEATREQFALLRTYLDSRHAGGGMSDMGLFDYVAMVEETPVNTHIIEYRRRGEDGQPG
                     ALVACALSDVLRDGLSMVYSFFHPGEDARSLGTHMILDHVRAAQMRGLKHVYLGYWVQ
                     GSEKMDYKARFRPMEALGREGWERL"
     gene            complement(1452..2387)
                     /locus_tag="VG274_04010"
     CDS             complement(1452..2387)
                     /locus_tag="VG274_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWE05847.1"
                     /translation="MAGENAVIIKRVKKGGHGGHHGGAWKVAYADFVTAMMAFFLLMW
                     LINTTTPEQKRGIADYFAPQSIAQTLSGSGGVLGGQVMGQESARAGGAASVFQKNSPA
                     SPNTKSKSTQAQTSQGGAANSEGQSSDMQSGSGNANDRSTSSSQDGDFAHAAEAIHQA
                     IQDNPDLAQMSKQVIMDQTPEGLRIQIVDQDGRPMFQQGTSEPMPYTKRLIAEVGKII
                     DRLPNRVSISGHTEATPFAGPGGKTNWELSADRANAGRALLAAGGLNSDRIYEVSGKA
                     GSEPLLPEDPNGSANRRLSITLLREAPPVAPGHQL"
     gene            2518..2994
                     /locus_tag="VG274_04015"
     CDS             2518..2994
                     /locus_tag="VG274_04015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWE05848.1"
                     /translation="MSFDTKAFRHALGSFPTGVAVVTAVVGAEPVGMTVTSFTSVSLD
                     PPLVLWCLKKDSHRYKVFAEAESFTINVLGTEHENVSARLARQGEHRLTDIDRLETEL
                     GPPALADAHAVFECARESVHDAGDHLILVGRVLRFARRDDGAPLVFYRGRYGALSG"
     gene            complement(2973..>4488)
                     /locus_tag="VG274_04020"
     CDS             complement(2973..>4488)
                     /locus_tag="VG274_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05849.1"
                     /translation="WSENDEKDYRDFIVGIGQSDCSTLDACLHSTANPFRDTDEPGVM
                     FQSDCAELPYILRFYFAWKRGLPFSFESAVSARSGATRDLRYSRNGNKVTERFDVPNG
                     ADGYRIIERLRGEISSASYRVHPDLESPLEPDLYAPAITPASIRPGTIVYDPAGHVGV
                     VFDIDAKGRVHFFDAHTDFSLTQMTYDLRFTRMKPSVGAGFKNWRPTRLVGAKRRADG
                     VLVGGHIDVASNKDIADFSDEQFFGNGKRPDDANWKSGTFTLNGETLDYYDYVRAQLA
                     GGKLTFEPVAEIGDMARSNCLDLRYRAQAVALAVAKGIQNQLEPRRLPDNIYGADGDW
                     EMYSTPSRDARLKTAFKYLRDMAQRFVEMGARHDAHLFYNGDNLPTDLLRAYDQATRY
                     CRVTYIASDGSRVTLGYDDARQRLFAMSFDPYQCVELRWGDPSASGCADGSVKRAWYE
                     AEQSLRNQLERTYDARMDFTLDDLQARSPGVTSSPDTDVRAYLLSVSAQPDKAP"
BASE COUNT          791 a         1377 c         1408 g          912 t
ORIGIN      
        1 gtcgcgtccg aaactcgcgc ataccggcga tatcggcgcg atgtatgtgc gcgatagtgc
       61 acgcggcacc ggcctcgccg atgcgttgat gacagcactt ctgaggcacg catcgacagt
      121 tgtcgagcaa gtccagctca ccgtgaatgc cgagaacacg cgcgcgataa aattctatct
      181 gcgtcacggt ttcaggcaga tcggcatcat tccgcgctcg cttcacgtcg gcgaccgcta
      241 ttacgacgat atgctgatgt tcaggtgtgt gtcgtcaggc gcctgatttt ttgcaaggcc
      301 agatcgcttc ggctgctttc gcgatgcctt gctctaccgg caacggatgc agttccgtgt
      361 gtgcgttcag ccagatcacg gtgtcgcgcg ccatcgtgcg tgcgctcggc cgtttggcga
      421 ggcaggttcg tgaaaggcct ttcgcgttca cgtcccaacg catcgcgccg gtgaccatgt
      481 cggcacattc aggcttttcc atctgacact gcgcaacgaa ataggcggtg attgtggccg
      541 gtttggggag gccgctttcc gtcgtctcgc gccagatgac ggccacggca gaggccgcgc
      601 agagtgctgc tatcgtgaac agtcccgccg tgaagaacga gacgcgcatt gcgctatagc
      661 cgctcccagc cctcgcggcc gagtgcctcc atcgggcgga agcgggcttt gtagtccatc
      721 ttttcgctgc cctggaccca gtagccgaga tagacgtgct tcaggccgcg catctgcgcc
      781 gcgcgcacgt ggtcgaggat catgtgtgtg ccgaggctgc gcgcgtcctc gccgggatgg
      841 aagaagctgt agaccattga aagcccatcg cgcaggacat cgcttaatgc acaggcgaca
      901 agtgcgcccg gctgaccgtc ttcgccgcgg cggcggtatt cgatgatatg cgtgttgact
      961 ggcgtctcct cgaccatggc gacgtagtcg aacaatccca tgtccgacat cccaccgccg
     1021 gcatggcgtg aatcgagata ggtgcgcagc agcgcgaatt gctcgcgcgt cgcctcggct
     1081 tcgacttcgg tgcgcacgag atcgttgttg cgtttgagga tgcgcttctg cccgcgcgag
     1141 aattccatct cgtcggcgat gatgcgcacg gagacacagg ccgaacatcc ttcgcaagcc
     1201 gggcgataag cgatgctctg gctgcggcgg aaaccggaat gggtcagcgc ttcgttcagc
     1261 ggctgggcca gcgcgccggt gagccgcgcg aacaccttgc gctccgtccg ccccggcaaa
     1321 taaggacatg ccccccccgg tgtcaggaag aattgcggaa cgcgggcatc gcgctggttg
     1381 gtcacgtcta tctcactctt gaacggtgat tatgaccaag aattcgtatg tcacgccagt
     1441 ggcttgacag attagagttg atggccaggc gccaccggcg gcgcctcgcg cagcagcgtg
     1501 atgctgagcc gccggttggc cgagccgttc ggatcctcgg gcagtaacgg ttccgatccc
     1561 gccttgccgg aaacttcgta aatgcggtcg gaattcaaac cacccgccgc gagaagcgca
     1621 cgccccgcat tcgcgcggtc cgcggacagt tcccagttgg tcttgccgcc cggaccggcg
     1681 aacggagtcg cctcggtgtg gccgctgatc gagacccggt tgggcaggcg gtcgatgatc
     1741 ttccctacct ctgcgatgag acgcttggtg tacggcatcg gctcggacgt gccttgctgg
     1801 aacatcgggc gtccgtcctg atccacgatc tggatgcgca ggccttccgg tgtctggtcc
     1861 atgatgacct gcttggacat ctgcgcgaga tcgggattgt cctggatcgc ctgatggatc
     1921 gcttccgcgg cgtgcgcgaa gtcgccgtcc tgcgaggacg atgtcgaacg atcgttggcg
     1981 ttgccactgc ccgactgcat gtcggacgac tgaccttcgc tgttggccgc accgccctga
     2041 ctggtctgcg cctgcgtcga tttggatttg gtgttgggcg aggcgggact gttcttctgg
     2101 aacacggacg cggcaccacc ggcgcgcgcg ctttcctgcc ccatgacctg accgccgagc
     2161 acgccgcccg atccgctcaa tgtctgggcg atgctctgcg gcgcgaaata atcggcgatg
     2221 ccgcgcttct gttccggcgt cgtggtgttg atcagccaca tcagcaggaa gaacgccatc
     2281 atcgcggtca cgaagtccgc ataggcaacc ttccacgcgc cgccgtgatg gccgccgtgc
     2341 ccgccttttt ttacgcgttt gataatgacg gcgttttcgc cagccatgct tttatcccac
     2401 cgaatgaccc gttgcccggc tggaaagcgg tctttgccgc ccttgcctcg tccgggttga
     2461 tggggacaat gcccgatacc ctgttaagca agcgttgaaa cccggcgaaa cgacgaaatg
     2521 agcttcgata ccaaggcctt ccgccatgcg ctgggcagct ttccgaccgg cgtggcggtc
     2581 gtcacggcgg tggttggggc cgagcctgtc ggcatgacag tcacctcctt cacctcggtc
     2641 tcgctcgacc cgccgctggt cctgtggtgc ctgaagaagg attcccaccg ttacaaagtc
     2701 ttcgccgagg cggagagctt cacgatcaat gtgctcggca ccgaacacga aaacgtgtcc
     2761 gcgcgcctcg ccagacaggg cgagcatcgt ttgaccgata tcgatcggct ggagaccgaa
     2821 ctcgggccac ccgcgctcgc cgacgcgcat gcggttttcg aatgcgcgcg cgaatccgtc
     2881 cacgacgctg gcgatcatct gatcctggtt ggccgtgtgc tgcggtttgc ccgtcgcgac
     2941 gatggcgcgc cgctggtttt ctatcgcggc cgctatggcg ccttgtcggg ctgagcggag
     3001 acgctcaaca gataggcgcg gacatccgta tcgggtgacg atgtcacgcc cggactgcgc
     3061 gcttgaagat cgtccagcgt gaaatccatg cgcgcgtcat aggtgcgctc gagttgattg
     3121 cgcagcgatt gctccgcttc ataccaggcg cgtttgaccg atccgtctgc gcagcccgaa
     3181 gcgctgggat cgccccagcg cagctccacg cactgatagg ggtcgaacga cattgcgaac
     3241 aggcgctggc gcgcatcgtc gtagcccagc gtaacgcgac tgccgtcgct cgctatgtag
     3301 gtcacgcggc aatagcgcgt cgcctggtcg taggcgcgca gcagatcggt ggggagattg
     3361 tcgccgttgt aaaagagatg cgcatcgtgg cgcgcgccca tctcgacgaa acgctgcgcc
     3421 atatcgcgca gatatttgaa ggcggtcttc aatcgagcgt ctcgcgacgg cgtcgaatac
     3481 atttcccaat cgccatcggc gccgtagata ttgtcgggaa gccgccgcgg ctcgagctgg
     3541 ttctggatgc ccttcgcgac tgcgagtgca actgcttgcg cgcgatagcg gaggtcgagg
     3601 caattggagc gcgccatgtc gccgatttcc gccaccggct cgaaggtgag ctttccaccg
     3661 gcgagttggg cgcgcacata gtcatagtaa tcgagcgtct cgccgttcag cgtgaaggtg
     3721 ccgcttttcc agttcgcatc gtctggccgt ttgccgttgc caaagaactg ttcgtcggag
     3781 aaatccgcga tgtccttgtt cgacgcgacg tcgatatggc cgccgacgag tacgccgtcg
     3841 gcgcgccgtt tggcgccgac caggcgggtg ggccgccagt tcttgaagcc ggccccgacg
     3901 gacggtttca tgcgcgtaaa gcgcagatcg tacgtcatct gggtcaatga aaaatcggta
     3961 tgcgcgtcga agaaatggac gcggcccttg gcatcgatat cgaagacgac accaacatgc
     4021 cctgccggat cgtaaacgat cgtgccgggc ctgatcgaag cgggcgtgat cgccggtgca
     4081 tagagatcgg gctccagcgg cgattcaaga tcgggatgga cccgataaga tgccgaagaa
     4141 atctcgccgc gcagccgttc gatgatcctg tagccgtccg caccgttcgg cacatcgaaa
     4201 cgttcggtga ctttgttgcc gttgcggctg tagcgaaggt cgcgcgttgc gccgctacgc
     4261 gcgcttaccg cgctttcaaa cgagaatggc aagccgcgtt tccaggcgaa atagaagcgc
     4321 aggatataag gaagctccgc gcaatctgac tggaacatca cgcccggctc gtcggtatcc
     4381 cggaaaggat ttgccgtgct gtgcaaacaa gcgtcgagcg tactgcaatc cgattggccg
     4441 atccccacga tgaagtcgcg gtaatccttt tcgtcgtttt cactccag
//
LOCUS       DASZMC010000206         5472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_372416,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000206 DASZMC010000000
VERSION     DASZMC010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372416"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VG274_04025"
     CDS             <1..417
                     /locus_tag="VG274_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05850.1"
                     /translation="ATAFVPQLENRPSRTQWPDRALAGTFESIWQVETHRPLRIVAAD
                     GWLGGLIAMRSEPRPSVWTDASYAKSPWITPQAVTRDGALIVWRVKDDPQPPAAFQHL
                     PGLEVLGVKSFAWPRTPNAPPLRIGFGIIAPDPSRK"
     gene            547..2067
                     /locus_tag="VG274_04030"
     CDS             547..2067
                     /locus_tag="VG274_04030"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWE05851.1"
                     /translation="MSAAPVETEIKLLLKSGARAEIERHPAFAKALRADPPRHELTTY
                     FDSDDLALRRRGYSLRIRDLGGDRVQTLKKVPADGAEAHQRNEWEWKLPGNRLALRPL
                     GEVLDAEAELAGKYRRARSKFVTEISRRTYEILCEGAQIEAVIDEGVVRAGDSEEEVS
                     EIELEIKNGPPGPAYRLALDLLEHEPLSLGAESKADRGYRLLTGNETPAGKSRALALP
                     RGATLEAALKLTAESALRDFIANMPAAHGGDPEGVHQMRVALRRLRTMLVLFGPFLEP
                     CAKERFSDAVRDIAAVLGGARDWDVFADETLRAARQAGIHAEWIGAFEEQAAKRREYA
                     HREVRALIDGKGPAELVLGIEAWLSDAGWTAKLPSSAGTPVVKPMRDLLDRVARKVAR
                     RGRDAAALPPQELHPLRKSIKKLRYSSESAGAMYSDRRAKDYVKRCKKLQTILGAIND
                     AQVTLRLVQKMAPAHSAAGAPAAGAFHKWNLARQNEARKKLAKAWRKFERAEPFWQ"
     gene            complement(2112..2480)
                     /locus_tag="VG274_04035"
     CDS             complement(2112..2480)
                     /locus_tag="VG274_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05852.1"
                     /translation="MHRNRFAFALAATMLTGNMTSSAAQTASGGLVQPTRLAQGQSGG
                     AATSGGVASGGAAAETPAPPAAAPELAPGPPAGEHRAMFLSTDTLVAGGIVAAAVIVC
                     AITCFGNSTSTTTSTTVIHK"
     gene            2592..3554
                     /locus_tag="VG274_04040"
     CDS             2592..3554
                     /locus_tag="VG274_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HWE05853.1"
                     /translation="MTSVKSDQLLSLEAVREAAARIAPYVGLTPVIAMTPTGLRLKAE
                     NLHPVGAFKLRGAFNAILSLSQAERRRGVVAHSSGNHAQAVAYAAHKLGIKAAVVMPE
                     GASPAKLEATRHWGAEIHLVDAAGLDRAPTCAALAREHGYAIIEPYNSLAIMAGTGTI
                     ALEILEQCPGTRVVTVPVSGGGLIGGVAAAAAQSDSSIRIIGVEPELAADASDSFRAG
                     RIVRIDPQLAMRTMADGLRVPQLGALPFAQIRAFVHDIMTVSEDAIAEAMRRIAREAR
                     LVAEPSGAVAAAGALAAGLDPGTTVAILSGGNTSLDRYAAVLSA"
     gene            3620..4222
                     /locus_tag="VG274_04045"
     CDS             3620..4222
                     /locus_tag="VG274_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWE05854.1"
                     /translation="MTRLHRRGAAGFCAAILLAFGLSGCGVNAVPQQDEQVKAAWSEV
                     LNQYQRRTDLIPNLVATVKGYANQERTVLTQVTEARAHATQMTIPADILSNPQAFHQF
                     EQNQAQVGYALGRLLAVSERYPDLKSNENFLALQSQLEGTENRIAVARRDYIDAVQTY
                     NTTLRTIPSRWVAAILYPDAKVKQTFTISEQAQQAPKVQF"
     gene            4230..4979
                     /locus_tag="VG274_04050"
     CDS             4230..4979
                     /locus_tag="VG274_04050"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWE05855.1"
                     /translation="MQRIFLRSIFCGLAFLLLVAQGAPAAPVFPPLTGRVVDEAGILS
                     PSTQNNITAILAQEEKRTGDQIVVATLKSLQGYTIEQYGYQLGRAWGIGQKGKNNGAL
                     IIVVPSTHDVRIEVGYGLEGELTDAQSKIIIDEFMLPAFRRGDYDAGVTAGTQAVIKV
                     LGGVALQPNDDNAPDRERRSSGSGGIPWFLIVFLIWIVFGRFLWPLLFLGGSRGFGGG
                     FGGGFGGGGFGGGGFSGGGGSFGGGGASGRW"
     gene            4979..>5472
                     /locus_tag="VG274_04055"
     CDS             4979..>5472
                     /locus_tag="VG274_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05856.1"
                     /translation="MRRPLSTDEKGRILAAVAAAEARTQVHVAVSIVPASDRYLLYPL
                     IWAAVAALVAGGVLAFAQPHLALGEGFAIEALVFVALSFLLDWWPLRLLLVPRHVRDR
                     HAQSLAHREFAARVLASRERKGGVLFFVSLGERYAEILADRDTHERVGAAQWNRIVAD
                     YVDSA"
BASE COUNT         1018 a         1721 c         1771 g          962 t
ORIGIN      
        1 gccactgcct ttgttccgca attggagaac aggccgtccc gcactcagtg gccggaccgt
       61 gcgctggccg gaacatttga atccatctgg caagtcgaga cgcatcgtcc gcttcgaatc
      121 gtcgccgccg atggctggct cggaggtctc atcgccatgc gctccgaacc gcggccgtcg
      181 gtctggaccg acgccagcta cgcgaagtcc ccctggatca cgccgcaggc ggtgacgcgc
      241 gacggagcgc tcattgtgtg gcgcgttaag gacgatccgc aaccgccggc agcgttccaa
      301 catttgccgg gactggaggt tctcggcgtg aaatcatttg cgtggccgag aaccccgaac
      361 gcgccgccgc tccggattgg attcggcatt atcgcgcccg acccatcgcg caagtagcaa
      421 tcggcattgc acctcagccg ccatgatgtt gatgctcaaa tccatgggcg ggcgccgctg
      481 aggaacaagc ctcgttgctc ggagttcgaa tccagtcggc cgtggcaatg cgacggatga
      541 agtcagatga gtgcggctcc agtcgaaacc gaaatcaagc tcctgctcaa gagcggagca
      601 cgcgcggaaa tcgaacgcca ccccgcgttc gcaaaggcgt tgcgcgccga cccgccgcgc
      661 cacgaactga cgacctattt cgactcggac gatcttgccc tgcgccggcg cggctacagt
      721 ctgcgcattc gcgatctggg cggagatcgc gtgcagacgc tgaagaaagt accggccgac
      781 ggggccgagg cccatcagcg caacgaatgg gaatggaagc ttcccggcaa ccgtctggcc
      841 ttgcgcccgc ttggcgaggt tctcgacgcc gaggcggaac ttgccggcaa gtatcgccgc
      901 gccaggtcga aattcgtcac cgaaatcagc cgccgcacct acgaaattct gtgtgaaggc
      961 gcgcagatcg aagccgttat cgacgaaggt gtggtcagag cgggcgacag cgaagaagag
     1021 gtcagcgaaa tcgaactgga gatcaagaat ggtcctccgg gacccgccta tcggcttgca
     1081 ctggatctgc tcgaacacga acccctgagt ctgggcgccg agagcaaggc cgatcgcggc
     1141 tatcggcttt tgaccggaaa cgaaactccg gccggcaaat cgcgcgcatt ggctttgccg
     1201 cgcggagcca cgctcgaggc agctctcaaa ttgaccgccg aatcggcgct tcgcgatttc
     1261 atcgccaaca tgccggcggc gcacggggga gatcccgagg gcgtccacca gatgcgcgtt
     1321 gcgctgcggc gcctgcgcac gatgctcgtg ctctttggcc cctttctcga gccttgtgcg
     1381 aaggaacgct tcagcgacgc ggtgcgcgac atcgccgcgg tcttgggcgg cgcccgggac
     1441 tgggacgtgt ttgcggatga aacgctgcgg gcggcaaggc aggccggcat tcacgccgag
     1501 tggatcgggg cattcgagga acaggcagcg aagcgccgcg aatacgcgca tcgcgaggtg
     1561 cgtgcgctca tcgacggaaa aggtccggcg gagctggtgc tcggcatcga ggcgtggctc
     1621 agcgatgccg gctggacggc gaaattgccg tcaagcgcag gaacgcccgt cgtcaaaccc
     1681 atgcgcgacc tccttgaccg ggtggcacgc aaggtcgcgc ggcgcggacg cgatgcggcc
     1741 gctctcccgc ctcaggagct tcatcccttg cgcaaatcga tcaagaagct gcgctattca
     1801 tcggagagcg caggggcgat gtattccgac aggcgcgcga aggactacgt gaaacgctgc
     1861 aagaaattgc aaacgattct tggagcgatc aacgatgcgc aggtgacctt acgtcttgtg
     1921 cagaagatgg cgcccgcgca ttccgcggca ggggcgccgg ccgcgggcgc ctttcacaaa
     1981 tggaatctgg cgcggcagaa cgaggcgaga aagaagctgg ccaaggcctg gcgaaaattc
     2041 gaacgggccg agccgttctg gcaataggcc tggcatattt cgcccgttgc agcgcgcgac
     2101 cgccgcagaa actacttgtg gatcaccgtc gtcgacgtcg tcgtgctggt cgagttgccg
     2161 aagcaggtta tcgcgcacac gattacagcg gcggcgacga tgccgccagc gaccagcgta
     2221 tccgtggaca gaaacatcgc ccggtgctcg ccagctggcg gaccaggcgc cagttccgga
     2281 gcggcagccg gaggcgctgg agtctcggcg gcggccccgc ccgaagcaac gccccccgac
     2341 gtagcggccc cgccgctttg gccctgcgcc agacgtgtcg gctgaacgag cccaccagat
     2401 gccgtctgtg ctgccgacga tgtcatgttc ccggtcagca tcgtagctgc gagtgcaaat
     2461 gcgaaccggt tcctgtgcat cggaatcctc aaattcccgg accgccgatc ctctaccagc
     2521 caaaggttga aacgccccaa gagacgcaaa gctgcaaatg gacatcaaat catgctagtc
     2581 cctcgtgtgt gatgaccagc gtcaaatccg accagctttt gtctctcgaa gctgttcgcg
     2641 aagccgcagc ccggatagca ccctatgtgg ggctaacgcc cgtcatcgcg atgaccccga
     2701 ccgggttgcg cctcaaggca gaaaatctgc atcccgttgg ggcattcaag ttgcgcggcg
     2761 ccttcaacgc catcctgtcc ctttcgcaag cagagcgccg ccgcggcgtc gtcgcacatt
     2821 cgagcggcaa tcatgcccaa gcggtcgctt acgcggctca taagctgggc ataaaggccg
     2881 cggtcgtcat gccggagggc gcctcgccgg ccaagctcga agcgacgagg cactggggcg
     2941 ccgaaattca tctggtcgat gcggcagggc tggaccgcgc acccacctgc gcggcgctcg
     3001 cacgcgagca cggctacgca attatcgagc cttacaattc gctggcgatc atggcgggga
     3061 caggcacgat tgctcttgaa atcctggaac agtgccccgg cacgcgggtc gtcaccgttc
     3121 ccgtctcggg tggcggattg atcggaggcg tggcagcggc ggcggcacag tccgattcct
     3181 cgattcgcat aatcggcgtg gagccggaac tggccgcaga tgcaagcgac agcttccgcg
     3241 caggccgaat tgtgcgcatc gacccgcaac tcgccatgcg caccatggcg gatggacttc
     3301 gcgtccctca actcggagcc ctgcccttcg cgcagatccg cgcctttgtg cacgacataa
     3361 tgactgtgtc cgaagacgcc atcgccgaag cgatgcggcg gattgcgcgc gaagcgcggc
     3421 tcgttgccga accaagcggt gcggtggccg ccgcaggcgc tctggcggct gggctcgacc
     3481 cggggacgac cgtcgcgatc ctttccggcg gaaatacgag cctggaccgc tatgccgccg
     3541 tcctcagcgc ctagccttgg cacgggagac cccctgccta tatgagaatg cgggaacggg
     3601 tgtatcggga agggctctca tgactcgact acataggcgc ggcgcggccg gcttttgcgc
     3661 ggcaattctc ctggcctttg gcctttcggg ttgcggcgtg aatgccgtgc cgcagcagga
     3721 cgagcaggtc aaggcggcgt ggagcgaagt actgaatcag tatcagcgcc gcaccgatct
     3781 cattcccaat ctggtggcga cggtgaaggg ctatgccaat caggagcgga ccgtgctcac
     3841 tcaggtcacc gaggcacgcg cccacgcaac gcagatgacc attccggcgg acatcctgtc
     3901 caatccccag gccttccatc aattcgaaca gaaccaggcg caggtcggat acgcgctcgg
     3961 ccggcttttg gcggtgagcg agcgctatcc cgatctcaaa tcgaacgaga atttcctggc
     4021 gctgcaatcg caactggagg gtaccgagaa ccgcattgcg gtggcccggc gcgactacat
     4081 cgacgccgtg cagacctaca acacgacgtt gcgcaccatt ccgagccgat gggttgcggc
     4141 aatcctctat ccggacgcga aggtgaagca gaccttcacc atcagcgagc aggcccagca
     4201 ggcgcccaag gttcaattct gaccctctga tgcagcgaat cttcctgcgc tcgattttct
     4261 gcggactggc cttcctcctg ctcgtggcgc agggcgcgcc ggccgcgcct gtctttcctc
     4321 cgcttaccgg gcgcgtggtc gatgaggccg ggattctttc gcccagcacg cagaacaaca
     4381 tcacggcgat tctcgcgcag gaagagaagc ggacaggcga ccagatcgtc gtggccacgc
     4441 tcaaatcgct ccagggctac acgatcgagc aatatggcta tcagctcgga cgcgcctggg
     4501 gcatcgggca gaaggggaag aacaacggcg cgctgatcat cgtcgtgccg agcacgcatg
     4561 acgtccgaat cgaggtggga tatggcctcg aaggcgagct caccgacgcg caaagcaaaa
     4621 tcatcatcga tgaattcatg ctgcccgcct ttcggcgcgg cgattacgat gccggagtga
     4681 cagcgggaac gcaggcggtc atcaaggtgc ttggcggcgt cgccctgcag ccgaacgacg
     4741 acaacgcgcc ggatcgggag cggaggagca gcggcagcgg cggcataccc tggttcctca
     4801 tcgtgttcct gatctggatc gtcttcggcc gctttctatg gccgcttctc ttcctcggcg
     4861 gttcgcgcgg cttcggtgga ggtttcgggg gtggtttcgg cggaggcgga tttggcggcg
     4921 gaggattctc cggtggcgga ggttcgttcg gcggtggcgg cgcatcggga cggtggtgat
     4981 gcgcagaccg ctttccaccg acgagaaagg gcgcatcctt gctgcggttg ccgccgcgga
     5041 agcgcgcacg caggtgcacg ttgccgtatc gatcgtgcct gcgagcgatc gctatctgct
     5101 ttatccactc atctgggctg cggtcgccgc tttggtcgca ggcggcgtgc tggcattcgc
     5161 gcagccgcat cttgcgcttg gcgaaggttt cgcgatcgaa gcgcttgtct tcgtagcgct
     5221 cagttttttg ctagactggt ggccgcttcg tcttctgctc gtgccgcggc acgttcgcga
     5281 ccggcatgcg caatcgttag cgcatcgcga attcgcagcg cgtgttcttg caagccgcga
     5341 gcgcaagggc ggcgtgcttt tctttgtctc gctgggcgag cggtacgcgg aaatcctggc
     5401 cgatcgcgac acccatgaac gggttggcgc cgcgcagtgg aaccgcatcg ttgccgacta
     5461 tgtggactcc gc
//
LOCUS       DASZMC010000207         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5303273,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000207 DASZMC010000000
VERSION     DASZMC010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5303273"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VG274_04060"
     CDS             complement(<1..233)
                     /locus_tag="VG274_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05857.1"
                     /translation="MGYIEQSLGASETLVARARFHWIYKLQAWLTLVFLGIFIIGIWI
                     FFKMKIRMWTTEITVTTHRFIEKTGWFTLRTNE"
     gene            complement(258..533)
                     /locus_tag="VG274_04065"
     CDS             complement(258..533)
                     /locus_tag="VG274_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWE05858.1"
                     /translation="MSRSKFRPALLLADAGRGDWIACQITSNPYADERAIVLANDAFA
                     EGSLQRVSYARPGKLFTANESLLAARVGRLKPSELTQVREAVVALFR"
     gene            complement(560..769)
                     /locus_tag="VG274_04070"
     CDS             complement(560..769)
                     /locus_tag="VG274_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05859.1"
                     /translation="MLQTIEVEIDATGRVRALEPVGKLPVGRALLTLLPSSEDDASRV
                     AELSLAEDWLRPEEDAAWAHLQPGK"
     gene            complement(890..3283)
                     /locus_tag="VG274_04075"
     CDS             complement(890..3283)
                     /locus_tag="VG274_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWE05860.1"
                     /translation="MSRGRIIGAYQPRTRRAALADTLDDVRRALLHFVGTAVLRSAGA
                     LLFLIAAAGMLALATYSPGDGSLNNATSRDPSNLLGGLGATAADLLLQTFGLAALAAL
                     APPAIWGALGLAGKNIRHAGWRALAWPLGTALAAGGCGAFPPPVIMPAGAGGLIGIAV
                     FGLARHAAEASHQPWVVQAVPILLLLVGLPLAFLATGLRFQPIWRGARAAGSLFLRLG
                     GMLVRPRSQRAALPATTDDEWEEEDTDDGALDIAPEPAAARSRESERQTRVKRDDPRK
                     PLARPKPARQPALNLGESQYQLPPLGLLAEPVRIHDRAGLSDEALEENARMLEAVLGD
                     FGVRGRIVAVRPGPVVTLYEFEPAAGVKSSRVVSLSDDVARSMSAVAARIAVIPGRNV
                     LGIELPNLHRETVYLREMIGSGEYEKARAPLVLALGKTIGGEAVMADLAKMPHLLIAG
                     TTGSGKSVALNTMILSLLYRLPPDQARMILIDPKMLELSVYEGIPHLLAPVVTDPRKA
                     VVALKWAVREMEERYRKMSKLGVRGVEAFNERVVKAKEKGETLKRTVQTGFDRESGKP
                     VYEDETLELEPMPFIVVVVDEMADLMMISGKEIEGAVQRLAQMARAAGIHLIAATQRP
                     SVDVITGTIKANFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLYMMTGGRIRRVH
                     GPFVTDREVEEVVAFLKTQGTPEYLDAVTEEQDEDGDDPYGVFGSGGESGDDLYDKAL
                     AIVARERKATTSYIQRRLEIGYNRAARLVERMEEEGVISRPNHQGKREVLLPDHDER"
     gene            complement(3280..>4202)
                     /locus_tag="VG274_04080"
     CDS             complement(3280..>4202)
                     /locus_tag="VG274_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWE05861.1"
                     /translation="NGTREGLFLATFVTTPETKSGARPVILLPNPFYQCYAASILAAG
                     AEPVFVPALAENQFLPDYAALPEAILCRTSSAFLCSPSNPEGACASEEYWRTLFALAD
                     RYDFTVFADECYADIYFNEPPACALPVRFAQSGGFARLLTFHSLSKRSALPGLRSGIV
                     AGDPALIAKFRAFRNYAGPQVPMPIMAASAAAWNDEAHVAANRAAYAERFRLAGRVLG
                     NRFNLRLPAGGFFLWLDVGDGEVVALRLWREAGIRALPGAYMGRETEPGNPRSNPGFR
                     YIRVALVHELSTIEATLDRLADLVGEREMA"
BASE COUNT          751 a         1377 c         1350 g          724 t
ORIGIN      
        1 atctcgttgg tcctgagcgt gaaccagcct gtcttctcga tgaagcggtg cgtggtcacc
       61 gtgatctccg tcgtccacat gcggatcttc attttgaaaa agatccagat gccgatgatg
      121 aagatgccca gaaagaccag cgtgagccac gcctgcagct tgtagatcca gtgaaaccgc
      181 gcccgcgcga ccagcgtctc gctcgcccca agcgattgtt cgatgtagcc catattttcc
      241 tcccctcaag acgaacgtta tcgaaagagc gcaacaaccg cttcgcgcac ttgtgtaagc
      301 tccgacggct tcaaccgtcc cacacgtgcg gcgagaagcg attcgtttgc cgtaaacaac
      361 tttccgggac gggcgtaact cacgcgttgc aagctgccct ctgcaaatgc atcattcgcg
      421 agcacgatcg cccgctcgtc ggcataggga ttgctggtaa tctgacacgc gatccagtcg
      481 cccctgccgg catcggcgag caacagggct gggcgaaact tgctgcgact caaatccgaa
      541 aaagggaatg gcagtagaac tacttgcccg gctgcaaatg cgcccatgcc gcgtcctctt
      601 cgggtctgag ccagtcttcc gccagagaaa gctcggcaac gcgggaggcg tcatcttcac
      661 tcgacggcag caaagtcaga agtgcccgtc ccacgggcag cttgccgacc ggctcgagcg
      721 cccgaacgcg acctgttgcg tcgatctcaa cttcgattgt ctgcagcatc atcgcacctt
      781 tggcacgcat attaacacgc gaccgagctt accaaagtgc gtgatggcct gccaatccgg
      841 cccgcgcaag ggcgccggtt tggaataccg tcgataacag caagcgtatt caccgctcgt
      901 cgtggtccgg cagcagcacc tcccgcttcc cctggtggtt ggggcgggag atgacgcctt
      961 cctcttccat ccgctcgacc aggcgcgcgg cgcggttgta gccgatctcg aggcggcgct
     1021 ggatgtagct cgtcgtcgcc ttgcgttccc gcgccacgat cgccagcgcc ttgtcgtaga
     1081 gatcgtcgcc ggattcgccg ccgctcccga aaactccata gggatcgtcg ccatcctcgt
     1141 cctgctcctc ggtgacggcg tcgagatatt cgggcgtgcc ctgcgtcttg aggaaggcga
     1201 ccacttcctc gacttcgcgg tcggtgacga agggcccgtg cacgcggcgg atgcgcccgc
     1261 ccgtcatcat atagagcatg tcgccctggc cgagcagctg ctccgcgccc tgctcgccca
     1321 gaatcgtgcg gctgtcgatt ttcgaggtga cctggaagct gatgcgggtg gggaaattcg
     1381 ccttgatcgt gccggtgatc acgtccaccg acggacgctg cgtcgccgca atcagatgga
     1441 tgccggccgc gcgcgccatc tgcgccaggc gctgcaccgc gccctcgatc tccttaccgg
     1501 agatcatcat caggtcggcc atctcgtcga ccaccacgac gataaacggc atgggctcca
     1561 actcgagcgt ctcgtcctcg tagacgggct ttcccgactc gcggtcgaag cccgtctgca
     1621 ccgtgcgctt gagcgtttcg cccttctctt tcgctttcac gacgcgttcg ttgaaggctt
     1681 cgacgccgcg cacgccgagc ttcgacatct tgcgatagcg ctcttccatt tcgcgcaccg
     1741 cccatttgag cgccaccacg gccttgcgcg gatcggtgac gacgggagcg agcagatggg
     1801 gaattccttc gtacacggac agttccagca tcttgggatc gatgaggatc atccgcgcct
     1861 ggtcgggcgg caggcggtaa agcagggaca ggatcatggt gttgagcgcg acggatttgc
     1921 ccgagcccgt agtgccggcg atgagcaggt ggggcatctt ggcgaggtcg gccatcaccg
     1981 cctcgccacc gatggtcttg ccgagcgcca ggacgagcgg cgcgcgcgcc ttttcgtatt
     2041 cgccgctgcc gatcatctcg cgcaaataca ccgtctcgcg atgcaggttc ggcagctcga
     2101 tgccgagcac gttgcgcccc gggatgaccg caatgcgcgc ggcgaccgcg ctcatcgaac
     2161 gcgccacgtc gtccgacagg ctgaccacgc gggaggattt gacgccggcc gccggctcga
     2221 attcgtacag tgtcaccacc ggcccgggcc tgacggcgac gatgcgcccg cgcacgccga
     2281 aatcgccgag cacggcttcg agcatgcggg cgttctcttc cagcgcttca tcgctcaagc
     2341 cggcgcgatc gtgaatccgc accggttccg cgagcagccc gagcggcggc agctggtatt
     2401 ggctctcgcc gagattcagg gcgggctggc gcgcaggctt cggccgcgcg agcggtttgc
     2461 gcggatcatc gcgcttcacg cgggtctggc gttcgctttc gcggctgcgc gcggcggcag
     2521 gttccggcgc gatgtcaagt gcgccgtcat ccgtgtcttc ctcttcccat tcatcgtcgg
     2581 tggtagcggg cagtgcagct cgctgcgatc gcggacgcac aagcattccg cctaggcgca
     2641 ggaaaaggga gcccgccgcg cgggcgcccc gccagatcgg ctggaatcgg agtccggtcg
     2701 cgagaaaggc gagaggaagg cccaccagca aaagcaagat cggaacggct tgaaccaccc
     2761 agggctggtg cgaggcttcg gctgcgtgtc gggcgagacc aaagacggcg atgccgatca
     2821 gaccgcccgc gcccgccggc atgatgaccg gcggaggaaa ggcgccgcac ccgcccgcgg
     2881 cgagagcggt cccgagcggc caggcgagcg cccgccatcc ggcatggcga atgttttttc
     2941 cggcgaggcc gagcgcgccc cagatggccg gaggcgcgag cgccgcaagg gccgcgagac
     3001 cgaaagtctg cagcagcaga tccgcggccg tggcgccgag gccgccgagc agattgctcg
     3061 gatcgcgcga cgtcgcgttg ttcaggctgc cgtcgcccgg actgtaggtc gcaagcgcca
     3121 gcatgcccgc agctgctatg aggaagagaa gcgcgcccgc gcttcggagc acggcagtgc
     3181 caacgaaatg cagcaacgcc cggcgcacat cgtcgagcgt atcggccagc gctgcgcgcc
     3241 gtgtccgcgg ctggtaggcg ccgatgatgc gtccccggct catgccatct cccgttcgcc
     3301 gacgagatcg gcgagccgat ccagtgtggc ctcaatggtt gacaattcgt gaacgagagc
     3361 cacccgaata tagcgaaatc ccggattcga gcgcggattc ccgggttcgg tctcgcggcc
     3421 catataggcg ccggggagag cgcggatacc cgcttcgcgc cagagcctga gcgccaccac
     3481 ttccccgtcg ccgacatcga gccagagaaa gaatccgccg gcgggcaggc gcagattgaa
     3541 gcggttaccg agaacccgtc ccgccagccg gaaccgttcc gcataggcgg cgcgattggc
     3601 tgcgacatgg gcttcgtcgt tccatgccgc cgccgacgcc gccatgatcg gcatcggcac
     3661 ctgcggtccc gcatagttcc ggaaagcccg gaacttggcg atcagcgcgg ggtcgccggc
     3721 gacgattccc gatcggaggc ccggaagcgc cgagcgtttc gacagggagt gaaacgtcag
     3781 cagacgcgcg aacccgccgc tctgcgcaaa ccggacgggc agcgcgcagg cgggcggctc
     3841 gttgaaataa atgtcggcgt aacattcgtc cgcgaacaca gtaaagtcgt accgatcggc
     3901 cagcgcgaac agtgttcgcc aatactcttc gctggcgcag gcgccttcgg gattggaagg
     3961 tgaacagagg aacgcgctgc tcgtccggca gaggatcgct tcgggcaacg cggcgtagtc
     4021 gggaaggaat tggttttccg cgagggctgg cacgaaaacc ggctctgcgc ccgccgccag
     4081 gatcgatgcg gcgtagcatt gatagaacgg attgggcagc aggatgacgg ggcgggcgcc
     4141 ggacttcgtt tccggtgtcg tcacgaatgt cgcaagaaag agtccctcgc gcgttccgtt
     4201 ca
//
LOCUS       DASZMC010000208         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1204898,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000208 DASZMC010000000
VERSION     DASZMC010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204898"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..785)
                     /locus_tag="VG274_04085"
     CDS             complement(231..785)
                     /locus_tag="VG274_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWE05862.1"
                     /translation="MLIAFGDSISAGYGVATGRSFPDDLQRKLDAAGYAWRVVNMGVS
                     GDTTQGGVARIKTAIAVKPSLVLLELGGNDGLRGLPLAAARANLDQMIAAFEHNGAKV
                     VLAGMKIPPNYGPDYTTEFEKMYSDLAAKYKLTFIPFIMSDMMSPDLHYFQPDHIHPT
                     AEGAEIVANTVLAAIKPLLGAARH"
     gene            1070..1696
                     /locus_tag="VG274_04090"
     CDS             1070..1696
                     /locus_tag="VG274_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE05863.1"
                     /translation="MDLEVAAGQFVAIMGASGSGKSTFLGLLAGLDTPTSGRVVIDGV
                     DITGLNEDALALVRGRKIGFVFQSYQLIPTLTAEENVLLPHELTGGDVAAGIPRARDL
                     LTSVGLANRMDHYPVQLSGGEQQRVALARAFMVKPPILMADEPTGNLDSVNGAHVLDL
                     LIELNRREATTLILVTHDQTLAARAGRIVTLSDGLVTSDVIREQAVVK"
     gene            1764..>2050
                     /locus_tag="VG274_04095"
     CDS             1764..>2050
                     /locus_tag="VG274_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05864.1"
                     /translation="MAKFLFVVLAVAAGVGALAGVRGFSQTFRATLKDESRTVMAADL
                     TARQFAPASDAQAAKLDALAHRGVDHTLITETISMASSGIAAPGTDAATPAL"
BASE COUNT          377 a          638 c          621 g          414 t
ORIGIN      
        1 gtttcgtacg tgcccaggcg cggagccttg cccgtagggt tgttgcgacg tttgccgccg
       61 ttccttttag ttactgctta gtcactgcct ttgcgacgcc cgagctcaaa ccaaaactga
      121 aagctacgaa tccacacacg ccgagcgcgc tcgctcgtag cattttagaa aattgcatgg
      181 gattggcctc cggaaaatcg atcttgcgat ccagaataca ccgcgcggcg ttagtgtcgc
      241 gcagcgccca gcagcggctt gatggccgcc agcaccgtgt ttgcgacgat ctccgcgcct
      301 tcggccgttg ggtgaatgtg gtccggctgg aagtagtgca ggtccggcga catcatatca
      361 ctcatgatga acgggatgaa cgtcagcttg tacttcgcgg cgagatcgga atacatcttt
      421 tcgaattcgg ttgtgtaatc cgggccgtag ttgggcggaa tcttcattcc ggccagcacg
      481 accttcgctc cattgtgctc gaatgccgcg atcatctgat ccagattggc gcgcgctgct
      541 gccagcggca gtcctcgcag cccgtcattg ccgccgagtt ccaacagcac cagcgaaggc
      601 ttcaccgcga tcgccgtctt gatgcgcgcc acgccgccct gggtggtatc gccactgacg
      661 cccatgttga ccacgcgcca cgcgtagccg gcggcatcca gcttgcgctg caaatcgtcg
      721 ggaaagctgc gcccggtcgc gacgccgtag cccgcgctga tactatcgcc gaacgcgatc
      781 agcactttcc tcgtatcttc ttttgaacgt gcttccgttg gaagcacttc ttttgagcgc
      841 acctctgaag gaagcgcttc ttttgaacgc gcctgcgccg cgggtgtctg tgcgaacgca
      901 ccgggcgcca gcgaggcgat cgaaaggaaa ttccaaagta cgacagtgag agacagcagg
      961 cgatgcatct gctgccatca taataaatat agagattgcc attcttcagg cgaccggcat
     1021 taccaaatcc atcgacacgg gccgccaccg cgtcgaaatt ctgcgcggca tcgatcttga
     1081 agtcgccgca gggcaattcg tggcgatcat gggagcctcc ggcagcggca agagcacgtt
     1141 ccttggtttg ctggcgggac tcgacacgcc cacctcgggc cgcgtcgtca tcgacggcgt
     1201 ggatatcacg ggcttgaacg aagacgcgct cgcgctggtg cgcggccgca agatcggatt
     1261 tgttttccag tcctatcaat tgatcccgac gctcacggcc gaggagaacg tgctgcttcc
     1321 tcacgagctc accggtggcg atgtcgccgc gggcattccg cgcgcgcgcg acctgctcac
     1381 cagcgtcggc ctggccaatc gcatggatca ctatcccgtg caactatccg gcggcgagca
     1441 gcagcgcgtg gcgctcgccc gtgcatttat ggtgaagccg ccgatcctga tggccgacga
     1501 gcccaccggc aatctcgaca gcgtgaacgg ggcgcacgtc cttgatttac tgatcgagtt
     1561 gaatcggcgc gaagccacga cgttgattct tgtcacgcac gatcagacgc ttgcggcgcg
     1621 cgcgggccgc atcgtcacat tgagcgacgg cctcgtcacc agcgacgtca tccgcgagca
     1681 ggcggttgtg aagtgagact ccctgcatga ggctttctct tcaaaccgcg ggccggattg
     1741 cgtggcgtga aacacgatcg tccatggcaa agtttttatt tgtggtgctg gccgtggccg
     1801 ccggtgttgg cgcgctggcc ggcgtgagag gcttcagtca aacgtttcga gcgacgctga
     1861 aggatgagtc gcgcacggtg atggcggccg atctcacagc gcggcaattc gcgcctgcca
     1921 gcgacgcgca ggcggcgaag ctcgacgcgc tggcgcatcg cggcgtggat cacacgctga
     1981 tcacggaaac gatttccatg gcatcctccg gcatcgccgc gccgggtacg gatgccgcca
     2041 cgcccgcgct
//
LOCUS       DASZMC010000209         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3392502,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000209 DASZMC010000000
VERSION     DASZMC010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3392502"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..872)
                     /gene="hisF"
                     /locus_tag="VG274_04100"
     CDS             complement(93..872)
                     /gene="hisF"
                     /locus_tag="VG274_04100"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWE05865.1"
                     /translation="MLTKRIIACLDVRAGRVVKGIQFVDIVDAGDPAELAHRHAAAGA
                     DEIVLLDITATHEGRGTLLETVKRTAANLFVPFTVGGGIRSAEEAAAVFDAGADKVSI
                     NSAAIARPELIGEIGSSFGAQAVIVAIDARRATGSSDPVGEAEVYVSGGRKPTGRRVI
                     EWAREAEQRGAGEILLTSMDCDGMRDGFDCELTRRVSEAVQIPVIASGGAGNAQHFAE
                     VFEAGKADAALAASIFHFGITDSRALKAEVAAVGVPMRLPC"
     gene            complement(866..1501)
                     /gene="hisH"
                     /locus_tag="VG274_04105"
     CDS             complement(866..1501)
                     /gene="hisH"
                     /locus_tag="VG274_04105"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWE05866.1"
                     /translation="MIAVIDYKAGNLTSVVKALRFLGGSDIVVTQSPDDVLRANKIVL
                     PGVGHFRATQLLDDLQLTKAMREAVAKGAPFLGICVGLQWLYEGSTEAPETRGLAHFA
                     GMCERFPAIYNDEELKSPHVGWNSLENIRPDSRLLAGIEAGSFVYFTHSWRAPLSADT
                     AATANYGGPFTAAVERGNVMGVQFHPEKSAETGLRVLKNFLALQRVEGNLC"
BASE COUNT          347 a          601 c          433 g          292 t
ORIGIN      
        1 cgcagtcgct tctacacaag gtttcttccg agtcgccatg cggttgctcc gcatgattgg
       61 atgcaaccca agctgtgcaa aaatcccgac gcctagcaag gtagccgcat cggcaccccg
      121 acggccgcaa cctccgcctt cagcgcgcgc gaatcggtga taccaaaatg aaagatgctt
      181 gccgccagcg cggcatccgc cttgcccgcc tcgaacacct cggcaaagtg ctgcgcattc
      241 ccggcgccgc ctgaggcaat caccggaatc tgtacagcct ccgagacgcg tcgagtcagc
      301 tcacagtcaa acccgtcgcg catcccgtcg caatccattg acgtcaatag aatttctccg
      361 gcgccacgct gctcggcctc gcgggcccac tcaatgacgc ggcgcccagt tggtttacga
      421 ccgcccgaga cataaacctc cgcctcaccg acaggatcac tcgagcccgt cgctcgccgc
      481 gcatcgattg ccacaatcac cgcctgcgca ccgaagctcg atccaatctc accgatcagc
      541 tccggccgcg caatcgccgc cgagttaatg gagaccttgt ccgcacccgc atcgaagacc
      601 gccgcagctt cttccgctga ccgaatgccg ccaccgaccg tgaacgggac gaacagattc
      661 gccgccgtgc gcttcaccgt ctcgagcaga gtcccgcgcc cctcatgcgt cgccgtaata
      721 tcgagcagca caatctcatc cgctcccgcg gccgcgtgac gatgcgccag ctccgccgga
      781 tcgccggcgt caacgatatc gacgaattgg attcctttga cgacgcgacc cgcgcgtacg
      841 tccaagcagg caatgatgcg tttcgtcagc acaaattccc ttccacgcgc tggagagcaa
      901 gaaagttttt cagcacacgc agtcccgtct cggccgactt ctccggatga aactgcacgc
      961 ccatcacgtt gccccgttcg acggccgcag tgaacggtcc accgtagttg gccgtggccg
     1021 cggtgtccgc cgagagcggc gcgcgccacg aatgcgtgaa gtagacaaag ctgccggcct
     1081 caatccccgc aagcagccgt gagtccggac gaatattctc cagcgagttc cagcccacat
     1141 gcggcgattt caattcctca tcgttgtaga tggcaggaaa gcgctcacac atacctgcaa
     1201 aatgcgccag cccgcgcgtc tccggcgcct ctgtcgaccc ctcatacagc cactgcaatc
     1261 caacacaaat ccccaggaaa ggtgcgcctt tcgcaaccgc ttcgcgcatg gctttcgtaa
     1321 gctgaagatc atccagcaac tgcgtcgccc gaaagtgtcc gacgcccggc agcacaatct
     1381 tgttcgcgcg cagaacatcg tccggcgact gcgtgacgac gatgtcgctt ccgccaagaa
     1441 accggagcgc cttcacgacg ctggtcagat tgcctgcctt gtagtcgatc accgcgatca
     1501 tcggcgcgct cgcttcgctt cggctcgcgg ccaatactgg aactcgtcct catacagctc
     1561 gccagaaaga tccgagacac gcaccgcaag ccgcgtctgc gcatctgcaa cagccttgtt
     1621 gtagatcgcc gggccaacct cctcgagaaa gaagttcagc agcaggccgg cgg
//
LOCUS       DASZMC010000210         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1206572,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000210 DASZMC010000000
VERSION     DASZMC010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206572"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..814)
                     /locus_tag="VG274_04110"
     CDS             complement(173..814)
                     /locus_tag="VG274_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWE05867.1"
                     /translation="MPERCTCGAVLPEDARFCHKCGKPQRDEPLIAEQQEETPAAPPV
                     VVAPPSVAAFESAAISFHNAPAVRIALIAGLAAFFCSVIAGQFALPQEFAFVWLAASG
                     FFAVYLYRRRTGQRLSMMNGARLGWICGLFGFIVVTLALCATAILLSDPQLVSAMRDQ
                     LRTHGLTEANADQMIQLFRSPSGILAALSVSFVLFSALPAFGGAIGAKLLDRE"
     gene            895..>1862
                     /locus_tag="VG274_04115"
     CDS             895..>1862
                     /locus_tag="VG274_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWE05868.1"
                     /translation="MTTHEPALAPTFNDVQAAARRIAHIAQHTPVMTSRSFNAASGVE
                     AFFKCENLQTGGAFKIRGASNFIYSIPEADRARGVVAFSSGNHAQAVAIAARSLGMQA
                     TIAMPNDAPKAKLAATKGYGAHVVFYDRHKDDRVAFGKKLAEESGATLVPPYDHPWIL
                     AGQGTAAMELIQDTMIDSAPSLDALVVCVGGGGLMSGSSISAKAMNPGIRCFGVEPVD
                     ANDTFLSLAAGKRVSIDPPATIADGLRSQIPGELTFPILQKNLEGVILVTEDEIRETF
                     RFLLTRMKILVEPSGCVSAAAVLFKKLPPGIKRAGVILSGGNVDYE"
BASE COUNT          376 a          604 c          550 g          332 t
ORIGIN      
        1 tgaaatgatc gcgatgctgg taatattcgt gggtgtcgcg ctggtgaagg gattcggaca
       61 gaagaggaaa aagaagaagt cagcagtcag gacctaggag tcagaataag caccgtggcg
      121 cacgcactcg tgcgtgccgc gtcgagactc ctctcgacgc ctccctgccc ttttattcgc
      181 gatccagcag cttcgcgcca atcgctccac cgaacgccgg cagcgccgaa aacagcacga
      241 acgataccga cagtgccgcc agaattcccg agggcgaccg gaaaagctga atcatttgat
      301 ctgcgttcgc ttccgtcaat ccgtgcgtgc gcaactggtc ccgcatggcg ctcaccagtt
      361 gcgggtccga taacaaaatc gccgtggcgc acaacgccag cgtcaccacg ataaagccga
      421 acaggccgca gatccatccc agacgtgcgc cgttcatcat cgaaagccgc tgccccgtgc
      481 gcctgcgata gagatacacc gcgaagaatc cgctggcggc cagccacacg aacgcgaact
      541 cctgcggcag cgcgaattgc ccggcgatca cggagcagaa aaacgcggca agtccagcga
      601 tcagcgcgat gcgcaccgcc ggggcattgt ggaaactgat cgctgcggac tcgaacgccg
      661 ccaccgatgg aggcgcgacc acgacgggcg gcgcggcagg cgtttcctcc tgttgctccg
      721 cgatcagcgg ttcgtccctc tgcggcttgc cgcacttgtg gcaaaaccgg gcatcttccg
      781 gtaggaccgc tccgcatgta caccgttcag gcacgcttcc agtgtgccac gttattctga
      841 agcatggcgc acaggcgtca gtccgttgtg cgcccgaaat tgccgccaac cgccatgacc
      901 actcacgaac cggctcttgc ccccaccttc aacgatgttc aggccgcggc ccgccgcatc
      961 gcccacatcg cccaacacac tcctgtaatg acgtcgcgca gcttcaacgc ggcgtctgga
     1021 gtcgaggctt ttttcaaatg cgaaaatctg caaaccggcg gcgccttcaa gatccgcggc
     1081 gcctcgaact tcatctactc catcccggag gcggatcgcg cgcgcggcgt cgtggcgttt
     1141 tcctctggca accatgcgca ggccgtggcc atcgcggcac gctccctggg catgcaggcg
     1201 accatcgcca tgcctaacga tgcgcctaaa gcgaagcttg cggcgacgaa aggctacggc
     1261 gcgcacgtcg tgttttacga tcgccacaag gacgatcgcg tagcgttcgg caagaagctg
     1321 gccgaagaat ccggcgcgac gctcgtcccg ccctacgatc atccgtggat cctcgccggg
     1381 caaggcacgg ccgcgatgga gctgattcag gacaccatga tcgacagtgc tccgtcgctc
     1441 gatgcgctgg tggtttgcgt gggcggcggc ggcttgatgt ccggctcgtc gatctcggcc
     1501 aaagcgatga atccgggcat ccgctgcttc ggcgtcgagc ccgtggatgc caacgatacg
     1561 tttctgtcgc tggctgccgg gaaacgcgtc tcgatcgatc ctcccgcgac catcgccgac
     1621 ggattgcgct cgcagattcc cggcgagctg acgtttccga ttttgcaaaa gaatctggaa
     1681 ggcgtgattc tggtgaccga ggacgaaatc cgcgagacct tccgcttcct gctcacacga
     1741 atgaagattc tggtggagcc cagcggctgc gtatcggcgg cggccgtgct gttcaaaaaa
     1801 ttaccgccgg gaatcaaacg cgcgggcgtc atcctctccg gcggtaacgt ggattacgaa
     1861 at
//
LOCUS       DASZMC010000211         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1739281,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000211 DASZMC010000000
VERSION     DASZMC010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739281"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..625)
                     /locus_tag="VG274_04120"
     CDS             complement(200..625)
                     /locus_tag="VG274_04120"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWE05869.1"
                     /translation="MQRTATAKWQGDLRGGAGSLTTASGVLSDTAYSFHSRFEQGKGT
                     NPEELLAAAHAGCFTMALSNQLGVAGLKAESLETKCTVTIDKQESGFAITSSHLELSA
                     RIPGASKEAFEKATNEAKAGCPVSRLFNTKITLDARLES"
     gene            complement(678..1601)
                     /locus_tag="VG274_04125"
     CDS             complement(678..1601)
                     /locus_tag="VG274_04125"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWE05870.1"
                     /translation="MTNPAASFEPYRRRLLGLAYRMLGSMADAEDAVQETYLRWHATD
                     RDKVSDPRAFLMTTTTRICLDMLTSARARREEYVGPWLPEPVVDTASLAPDSRTELAE
                     DLSIALLLTLDRLSPLERAAFLLHDVFDFSFSEVASALERSEAACRQLAGRARAHVRE
                     VRPRGAAPLAGSEEIDAKHTQLMSAFVAASRSGDLNALMRMLASDVRVVTDGGGKTAA
                     ALNVLDGADRAARFLVGATRKGWREDFTFRFASINGLPGVIVDGPEGPVQSAAFEMRG
                     DLIGALYVVRNPDKLRHLATAPRSHEGGPGV"
     gene            complement(1616..2194)
                     /locus_tag="VG274_04130"
     CDS             complement(1616..2194)
                     /locus_tag="VG274_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05871.1"
                     /translation="MIRWALRKAIGKFERDWNYDASYMREIIEASPRAAWLFSRAAAL
                     GRFRRDIPLEAWCAAGITAVRHEDCGPCTQLAVTMAERAGVKPAVLRAVLADNPEAMP
                     PDVALAWKFTRATLAHDAAADEYRDAIVKRWGRRALVSLAFAITTARIYPTVKYALGH
                     GKACMRVVTGGTPVMLDHGRVPAPAEVAAHTP"
     gene            complement(2307..>2576)
                     /locus_tag="VG274_04135"
     CDS             complement(2307..>2576)
                     /locus_tag="VG274_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWE05872.1"
                     /translation="TLNPYDWEAIRRSVQKTSRVMVAYEDNLSFGYGAEIVARIADEL
                     FSYLDAPVKRVAALDTWVGYHPDLEAAILPQTQDLVKTMNETLAY"
BASE COUNT          465 a          863 c          758 g          490 t
ORIGIN      
        1 cacaggtgtg gtggctcccg tggtgaaacc ggactggatt tcgaccattt cctggtagat
       61 atcgtgcgcc agatggcgat cggcttcagg cggcagcgat gccgcaatgg cgtttgccca
      121 aggcacgtga accgtgatca gaaccgcggc cagcgagtag aagaaacggc gcatgcgcca
      181 ttttcacacg ctttgccggt tacgattcga ggcgcgcgtc gagtgtgatc ttcgtgttga
      241 agagcctcga taccgggcac ccggctttgg cctcattcgt cgctttctcg aaagcttcct
      301 tgctggctcc cggaatgcgc gcactcagct ccagatggct cgatgtgatc gcgaagccgc
      361 tttcctgctt atcgatcgtc accgtgcact tcgtttcgag gctttccgct ttcaacccag
      421 ccaccccaag ctggttcgaa agagccatgg tgaagcaccc ggcgtgcgcc gcggccagaa
      481 gctcttccgg attggtccct ttgccctgct caaagcggct gtgaaacgaa taggccgtgt
      541 cggaaagaac gccactcgcg gtggtaagcg agcccgcgcc gcccctcaaa tctccttgcc
      601 attttgctgt tgcggttctt tgcatgggcg cattttatag cgcggaagaa ccgcatccgc
      661 aaaactggcg gatcttttca tacgcccgga ccgccctcgt gcgaccgtgg cgccgtcgcc
      721 aaatgccgca gcttgtccgg gttccgcacc acatacagcg ccccaatcaa gtcacccctc
      781 atctcgaacg ccgcggactg tacgggcccc tcgggtccgt ccacaattac gccgggcagg
      841 ccattgatgc tcgcaaagcg gaacgtgaaa tcttcgcgcc accctttgcg cgtggctcca
      901 accaggaacc gcgccgcgcg atcggctccg tccagcacgt ttagcgccgc cgccgtctta
      961 ccgccgccat ctgtcaccac acgcacgtcg ctcgcgagca tccgcatcag cgcgttgaga
     1021 tcgcccgagc gcgatgcagc gacgaatgcg gacatgagct gcgtgtgttt cgcgtcgatt
     1081 tcttccgaac ccgcaagcgg cgcggcgcct cgtggccgta cctcgcgcac atgcgcgcgt
     1141 gctcgaccgg caagctggcg gcacgcagct tcgctccgct cgagcgccga ggccacttca
     1201 ctgaacgaga aatcgaatac atcgtgcagc aggaaggcag cccgctcgag tggtgacagc
     1261 cggtccagcg tcagcagcaa cgcaatggat aaatcctcgg ccagttccgt gcggctgtca
     1321 ggcgcaagcg acgccgtgtc gactaccggc tccggcagcc aggggccgac gtactcctcg
     1381 cgccgcgcgc gcgctgatgt cagcatatcg agacaaatcc gcgtcgttgt ggtcattaga
     1441 aatgccctcg gatccgaaac tttgtcgcgg tcggtcgcat gccagcgcag gtatgtctcc
     1501 tgcactgcat cctcggcatc ggccatcgac ccgagcatgc gatatgcgag accaagcagg
     1561 cgccggcggt acggctcaaa gcttgcggcc ggattcgtca ttgtgcccgc ggaactcacg
     1621 gcgtgtgcgc cgccacctcc gccggcgcag gcacccggcc gtgatcgagc atcaccggcg
     1681 tgccgccagt gacaacgcgc atgcaggcct tgccatgccc aagcgcgtat ttgactgtcg
     1741 gatagatccg cgccgtcgtg attgcgaaag ccagactgac cagtgcgcgc cttccccagc
     1801 gcttcacgat cgcatcgcga tactcatcgg cggcggcatc gtgcgcgagc gtggcgcgcg
     1861 tgaatttcca cgccagcgca acatccggcg gcatggcctc cggattatcg gccagcaccg
     1921 cgcgcagaac cgcaggtttt acgccggccc gctccgccat cgtgacggcg agctgggtgc
     1981 aagggccgca gtcctcgtgg cgcacggcag taattcccgc tgcacaccac gcctcgagcg
     2041 ggatatcacg ccggaatcgt ccgagagcgg cggcacggga aaacagccat gcggcgcgcg
     2101 gactcgcctc gattatctcg cgcatatagc tggcgtcgta gttccagtcc cgctcgaact
     2161 tgccaatcgc ttttcgcagt gcccatcgga tcatgtcgtt tctcctgcct ccaactgtga
     2221 gacgggacaa ggctccggag tgtgacatga gtgctgcggc cggctgcagg agggcgttcc
     2281 gcctccagca gacgcaactt tacgccttaa tacgccagag tttcgttcat ggtcttcacc
     2341 agatcctgcg tttggggaag aatcgcggct tcaaggtccg gatggtagcc cacccacgta
     2401 tccagtgccg ccacgcgttt caccggcgcg tccagatatg agaacagctc gtccgcaatg
     2461 cgcgccacga tctcggcgcc gtatccgaaa ctcagattgt cctcgtaggc gaccatcacg
     2521 cggctcgtct tctgcacgct gcgccggatc gcctcccaat cgtagggatt gagcgt
//
LOCUS       DASZMC010000212         8578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4756549,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000212 DASZMC010000000
VERSION     DASZMC010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4756549"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VG274_04140"
     CDS             <1..909
                     /locus_tag="VG274_04140"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWE05873.1"
                     /translation="IEADESKLADVTTKFDCVVEKLFVSTTGTNVHAGEPLARVWIQT
                     PDTMMQIGPDVVTREIDYVVALQDKNPAEITAAERVLREYGLPNSTIAEIRRTGHATR
                     SVTITAPRDGIVLEKSAVEGMHFNTGDPLFKIADLSSVWLMADVEEQDLGNIRQDETA
                     RAALVAYPGRTFTGKVDFIYPTLMSNTRTGRVRIVLSNPDGSLRESMYANVAIDTPVS
                     SGAQVVVVPDSAVIDSGTRQVVLVERGQGRFEPRTVQVGAHGEGYAQILGGVKPGDQV
                     VVSANFLIDAESNLRAALSGFGGGNP"
     gene            906..4028
                     /locus_tag="VG274_04145"
     CDS             906..4028
                     /locus_tag="VG274_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWE05874.1"
                     /translation="MIAAIIRWSSRNVALVLMAAAAIVAAGIYAVWNTPLDAIPDLSD
                     PQVIVYTEYPGQAPQVVEDQVTYPLTTALLAVPRSQVVRGFSFFGVSFVYVVFQDGTD
                     IYWARSRVLEYLNFAQKRLPEGITPSLGPDATGVGWVFEYAVIGAQRTLADLRSIQDW
                     LVRYQLTKAEGVAEVASVGGFVKQYQAVVDPDKLRAYGIPLSKVVDAIRASNMDVGGR
                     TIEMTEREFMVRGHGYLRGTKDLEQIVLKNENGAPVLLRDVARVEIGPDERRGLTELN
                     GQGEVAAGIVLQRSGENALNVIKSVKEKVADIAASLPQGVQILPVYDRSNLIYRAIRT
                     LTRTLLEESIIVALVCIVFLMHARSALVAIIMLPIGVLISLIVMHAFGVTSNIMSLGG
                     IAIAIGAMVDAAIVMIENAHKRLERAPQGEARADTLVRAATEVGPALFFSLLVITVSF
                     VPVFTLEAQEGRLFKPLVYTYTFSLAGAALLSVTLVPVLMKLFVRGRIMPEQRNPLNR
                     FLIAIYRPVIDVVLRWRVGTMVIAAAILAVSLYPVLHTGSEFMPTLNEGTLFYMPVSL
                     PSMSITKAAQLIQVQDRIIKSFPEVESVFGKAGRADTATDPAPLEMFETVINLKPEDQ
                     WPKGMTVDQLTSEMNAALQFPGVSNAWTMPIKARIDMLSTGIRTPVGVKVFGNDLSEI
                     DKIARRIEAVVRKVPGTTSAFAERVTGGYYLNIEPDREKLARYGLSIADVQDVIKTAL
                     GGEMVTTTVEGRERYGVIARYPRDYRDDPLAIAHDVLVNTSDGANIPLGEVASVTVGP
                     GPPSIRTENAQLVGYIFVDMQGRDLGGYVEDAQRAVQDQVKLPPGYHVEWSGQFEYLE
                     RAKARLATVIPLTLFLIFLLLYFNFQRFTESAIVMLSVPFALTGGFWLVWLMGFNISV
                     AVAVGFIALAGVAAETGVVMLIYLDNALRAIMEKRKEQGRALTKDDLYHAIIEGAVER
                     VRPKMMTVMAIMAGLLPILWSTGTGSEVMQRIAVPMVGGMISSTLLTLLVIPAIYALV
                     KGRHLATAQA"
     gene            4048..4662
                     /locus_tag="VG274_04150"
     CDS             4048..4662
                     /locus_tag="VG274_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWE05875.1"
                     /translation="MGLYRKFVLPRLINLAMHNKELRPFRERVIGAAEGRVLEIGAGS
                     GLNLQFYGPGASEVMALEPDPKLMAMARHHAAEATCPVKFLEASAEEIPLEDQSIDSV
                     ITTWTLCTIPDAGRALREMRRVLKPVGRLLFVEHGFAPEPGVQKWQDRLTPIWKRIGG
                     GCHLNRPISALIERAGFRIDRLDTGYIHGPKPMTFMYEGAARPC"
     gene            complement(4671..5849)
                     /locus_tag="VG274_04155"
     CDS             complement(4671..5849)
                     /locus_tag="VG274_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWE05876.1"
                     /translation="MMRAIAHALELAAAMGWEILWPLILGFALSACVQAVVSHEEMSR
                     LLPDDRPKSILRALALGAASSSCSYAAVALARSIFRKGADFTAAMAFEMASTNLVVEL
                     SVVLVVLMGWWFAAAEFVGAPIMVAILVLLFRRFLSKRAVETAREQAEKGLAGRMEGH
                     AGMDMAVRDRGSILERLASREGFTATSHYFVMDWASVWIDIALGLLIAGALAAWVPPQ
                     FWQAFFLVHHPLLARIWGPLVGPLVSVISFVCSVGNIPLAAVLWNGGISFGGVIAFIF
                     ADLIVLPVLDIYRKYYGWRMMGFLLVTFYAAMAGAAFAVEALFGALGLIPKEHHALVV
                     EAAIRWNYTTWLNLLFLGVAAVLLWRFFRTGGPKMLRMMNAPGMTHSHAHGDHGSHSV
                     "
     gene            complement(6159..6515)
                     /locus_tag="VG274_04160"
     CDS             complement(6159..6515)
                     /locus_tag="VG274_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05877.1"
                     /translation="MAHSLPLRYDYQVSGNRDRLLELARRYVWWSPPDRFVSENRSRI
                     IAQVMEIGTWEDAHALLNEVGRSAFVNVLRDPPPGGLSTRSQAFWHHRLGLGGAPGPK
                     AARRIGPERGVDAVHG"
     gene            6582..7820
                     /locus_tag="VG274_04165"
     CDS             6582..7820
                     /locus_tag="VG274_04165"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HWE05878.1"
                     /translation="MPLNFPKGFLWGTATAAHQVEGGNVASDLWLMENVPQSLFAEPS
                     GDACDHYHRYPQDIALLKGLGFGAYRFSIEWARIEPEPGFYSRAALDHYRRMIAACRA
                     IGIVPMVTFHHFTAPLWFTRDGGWTEPGSIDHFARYCETAGRALGDLMLYACTINEAN
                     VPMMITAQREADRGARGESPERFAKIARRCGGEARHWAPFLHCDGRAASLQLIAAHRK
                     GYEALKSAGLSCPVGITLALLDMQAERGGEANRDAAERLIQGQFLESTRGDDFVGVQT
                     YTRIRFGPKGHLPPPRDAERTQMGYEFYPEALDATVRTAARVSGCPVLVTENGIGTED
                     DSRRIAYAKRAIEGLHAAIADGVDVRGYFHWSLLDNYEWLEGYRPKFGLVAVDRTTQA
                     RRPKPSGYWLGEVARNNALI"
     gene            complement(7827..8195)
                     /locus_tag="VG274_04170"
     CDS             complement(7827..8195)
                     /locus_tag="VG274_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE05879.1"
                     /translation="MTRLAETRKEKALVVEDEALIRMSVCDMLAEMGLETIDTGNGHE
                     GLTLLAEDPSIGMLIADLGLPDLSGEELVRRARGLRPELKIIISTGHSAARAQPAFAG
                     VAFLAKPFDISGLRRAVEGV"
     gene            8320..>8578
                     /locus_tag="VG274_04175"
     CDS             8320..>8578
                     /locus_tag="VG274_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE05880.1"
                     /translation="MKDFAGRSAVITGGGTGMGRQLALQLAAEGCSVALCDVCAETMA
                     ETKRLALSNAPQGTRVSTFIADVSIEEQVLAFAASVARDLDT"
BASE COUNT         1676 a         2740 c         2639 g         1523 t
ORIGIN      
        1 atcgaggcag acgaaagcaa actcgccgac gtcaccacga aattcgattg cgtcgtcgag
       61 aaactctttg tctcgaccac tggcaccaac gtgcatgccg gcgagccgct ggcgcgcgtc
      121 tggattcaga cgcccgacac gatgatgcag atcggccccg atgtcgtcac ccgcgaaatc
      181 gattacgtcg tggcgttaca ggacaagaac cccgctgaga ttaccgcggc cgagcgtgtg
      241 ctgcgtgaat acgggttgcc caattccacg atcgccgaaa tccgccgcac cggccacgcg
      301 acgcggtccg tcaccatcac cgcgccgcgc gatgggatcg tgctcgaaaa atccgccgtc
      361 gaaggcatgc acttcaacac cggcgacccg ctcttcaaga tcgccgatct ctctagcgta
      421 tggctgatgg ccgatgtgga ggaacaggat ctcggcaata tccgccagga cgagaccgcc
      481 agagccgcgc tggttgccta tcccggccgc acattcaccg gcaaggtcga cttcatctat
      541 cccacactca tgtcgaacac gcggaccgga cgcgtgcgca tcgtattgtc caatcccgac
      601 ggatccttgc gcgaatcaat gtacgcgaac gtcgcgatcg acacgcccgt atcatcgggc
      661 gcgcaggtgg tagtcgtccc cgactccgct gtcatcgaca gcggaacccg ccaggtcgtt
      721 ctggtggaac gcggtcaagg ccgcttcgag ccccgcaccg tgcaggtcgg cgcgcatgga
      781 gagggctacg cgcaaatcct gggtggcgtg aaaccgggtg accaggtggt cgtcagcgcg
      841 aatttcctta tcgatgccga aagcaatctg cgcgcggctc tctcgggctt cggcggcggc
      901 aatccatgat cgcggcgatc atccgctggt cgagccgcaa tgtcgcgctc gtcctgatgg
      961 cggcggctgc gatcgtggcc gccgggatat atgcagtctg gaacacgccg ctcgatgcga
     1021 ttcccgatct ctccgatccg caggtcatcg tctacaccga atatccgggc caggcgccgc
     1081 aggtggtcga ggaccaggtc acctacccgc tgaccaccgc gcttctcgcg gtgccacgct
     1141 cccaggtggt gcgtggtttt tcgttcttcg gtgtttcctt cgtctacgtg gtcttccagg
     1201 acggcaccga catctattgg gcgcgcagcc gcgtgctcga atatctgaac ttcgcgcaaa
     1261 aacggttgcc tgaaggtata acaccttctc ttgggccgga cgcaaccggc gtcgggtggg
     1321 tgttcgaata cgccgtcatt ggagcgcagc gcacactcgc cgacctgcgc agcatccagg
     1381 actggctggt ccgttatcag ctcaccaagg ccgaaggcgt ggccgaagtt gcgagcgtcg
     1441 gcggcttcgt caagcagtat caggcggtcg tcgatccaga caaattgcgc gcctacggca
     1501 ttcccctttc gaaggtcgtc gacgcgatcc gcgccagcaa catggatgtc ggcggccgca
     1561 ccatcgaaat gaccgaacgc gaattcatgg tgcgcggcca cggctatctg cgcggcacaa
     1621 aagacctcga gcagatcgtg ctcaagaatg aaaacggcgc tcccgtcctg ctgcgcgatg
     1681 tcgcgcgcgt ggaaatcggt ccggacgaac gccgcggact gacggagctg aacggtcagg
     1741 gcgaggtcgc ggccggcatc gttcttcaac gctcgggcga gaacgccctc aacgtcatca
     1801 agagcgtcaa ggaaaaggtc gccgacatcg cagcaagcct gccccaaggc gtgcagattc
     1861 tgcccgtcta tgaccggtcg aacctgatct atcgggcaat ccgtacgctg acacgcacgc
     1921 ttctggaaga aagcatcatc gtggcgctcg tctgcatcgt cttcctgatg catgcgcgga
     1981 gcgccctcgt cgccatcatc atgctgccca tcggggtgct gatctcgctc atcgtcatgc
     2041 acgcgttcgg cgtcacctcg aatatcatga gcctcggcgg catcgccatt gccatcggtg
     2101 cgatggtgga tgccgcgatc gtgatgatcg aaaacgctca caagcgcctc gaacgcgccc
     2161 cgcaaggcga agcgcgggcg gatactctgg ttcgcgcagc caccgaagtg gggccggcgc
     2221 tgttcttcag cctgctcgtg atcacggtct cgttcgtgcc ggtgttcact ctggaagcgc
     2281 aggagggccg cctcttcaag ccgctcgtct acacctacac gttctcgctc gcgggcgcgg
     2341 cgctactttc cgtcacgctc gtgccggtgc tgatgaagct ctttgtgcgc gggcgtatca
     2401 tgccggagca gaggaatccg ctgaaccggt tcctgatcgc gatctaccgc cccgtgatcg
     2461 acgttgtgct gcgctggcgc gtgggcacga tggtcatcgc ggctgccatt ctggcggttt
     2521 ctctctaccc ggtcctgcac acgggcagcg agttcatgcc aacgctcaac gaaggcacgc
     2581 tgttctacat gccggtgagc ctgcccagca tgtccatcac caaggccgcg cagctcatcc
     2641 aggtgcaaga ccgcatcatc aaatcgtttc cggaagtcga atccgtgttc ggcaaggccg
     2701 gccgcgccga cacggcgacc gatcccgcgc cgctggaaat gttcgagacc gtcatcaatc
     2761 tcaagcccga ggatcagtgg cccaaaggca tgaccgtcga ccagctaacc tccgaaatga
     2821 acgcagcgtt gcagttcccc ggcgtcagca acgcctggac catgccgatc aaggcacgca
     2881 tcgacatgct gtcgaccggt atccgcacgc ccgtcggcgt caaggtgttt ggcaacgacc
     2941 tgagcgagat cgacaaaatc gcaaggcgga tcgaagccgt ggtgcgcaag gttccgggga
     3001 caaccagcgc gttcgccgag cgggtgacgg gcggttatta cctcaacatc gagcctgacc
     3061 gcgaaaagct cgcccgctac ggcctcagca tcgccgacgt gcaggacgtg atcaaaaccg
     3121 cgctcggcgg cgagatggtg acgacaaccg tcgaaggccg ggagcgctat ggcgtcatcg
     3181 cccgctatcc ccgcgactac cgcgacgatc cgctggccat cgcccacgac gttttggtca
     3241 acaccagcga tggcgccaac atcccgctcg gcgaggtcgc atccgtcacg gtgggcccag
     3301 ggccgccatc catccgaacc gaaaatgccc agctcgtcgg ctacatcttt gtggacatgc
     3361 agggccgcga tctcggcggc tatgtcgaag acgcgcagcg cgccgtgcag gaccaagtga
     3421 agcttccgcc cggctatcac gtcgaatgga gcggtcaatt cgaatatctg gagcgcgcca
     3481 aggcgcggct tgcaaccgtc atccccctga cactctttct gattttcctt cttctctact
     3541 tcaatttcca gcgcttcacc gagagtgcta tcgtcatgct ctccgtgccc ttcgcgttga
     3601 ccggcggatt ctggctcgtc tggctgatgg gcttcaacat cagcgtcgcc gtggcggtcg
     3661 gtttcatcgc gctggccggc gtcgcggcag aaactggagt ggtgatgctg atctatctcg
     3721 acaatgcatt gcgcgcgatt atggaaaagc ggaaagagca agggcgggca ttgacgaagg
     3781 atgacctcta tcacgccatc atcgaaggcg cggtcgagcg cgtgcgcccg aagatgatga
     3841 cggtgatggc gatcatggcg gggctgcttc ccatcctctg gagcacaggc acggggtcgg
     3901 aggtgatgca gcgcattgcc gtgcccatgg tgggcgggat gatctcgtcg acacttctga
     3961 cgttgcttgt aattcccgcg atctatgcgc tggtgaaggg gcggcatctg gcgactgcgc
     4021 aggcgtaaac ggcgaaagga caaggcaatg ggcctctatc gaaaattcgt cctgccgcga
     4081 ctgatcaacc ttgcgatgca caacaaggag ctgcggccat tccgcgagcg cgtaatcggt
     4141 gcagccgaag gccgcgtgct cgaaatcgga gcgggctccg gattgaacct gcaattctac
     4201 ggacccggcg cgagcgaggt gatggcgttg gagcccgatc cgaaactcat ggcgatggca
     4261 cggcaccacg ccgcggaagc aacatgtccg gtcaaattcc tcgaagcgtc agccgaagag
     4321 attccactcg aagaccaaag catcgattcg gtcatcacga catggacgct ctgcacgatc
     4381 cccgatgccg gtcgcgcgct ccgggagatg cgccgggtgt tgaaaccggt cggacggctt
     4441 ctgtttgtgg aacatgggtt cgcacccgag ccaggcgtgc agaagtggca ggatcggctt
     4501 acgcccatct ggaagaggat cggcggcggg tgccacctga accgcccgat cagcgcgttg
     4561 atcgaacgcg cgggcttcag gatcgacagg ctcgacacag gctacatcca cggccccaag
     4621 ccgatgacgt ttatgtacga gggcgcggcg agaccttgtt gatcgtcctt tcagactgaa
     4681 tggctgccgt gatccccgtg ggcgtgagaa tgagtcatac ccggcgcatt catcatccgc
     4741 agcatcttcg gtccgccggt gcggaaaaat cgccacagca ggacggctgc gacgccgagg
     4801 aacaggagat tgagccatgt cgtgtaattc cagcggattg ccgcttcgac gaccaaagca
     4861 tgatgctcct tgggaatgag gcccagcgcg ccgaacaagg cctcgacggc gaaggctgcg
     4921 cccgccatcg ccgcataaaa cgtcaccagc aagaatccca tcatcctcca gccgtaatat
     4981 ttgcggtaga tgtcgagcac ggggagcacg atcaaatcgg cgaagataaa cgcgatgacg
     5041 ccgccgaagc tgatgccgcc gttccagagc accgccgcca gcggaatgtt tccgacggag
     5101 cagacgaacg agatcacgct gacgagcggt ccgaccaatg gtccccagat tctcgccagc
     5161 agcggatgat ggacgagaaa gaacgcctgc cagaattgcg gtggtaccca tgcggcaagc
     5221 gcgcctgcga tcaggagacc cagcgcgata tcgatccaca cggacgccca gtccatgacg
     5281 aagtaatggc tcgtcgcagt aaatccctcg cgcgaagcga gccgctccag aattgaaccg
     5341 cggtcgcgca cggccatgtc catgccggcg tggccttcca tccggcccgc cagtcctttc
     5401 tcggcctgct ccctcgcggt ttcgaccgca cgcttcgaca ggaagcggcg gaacagcagg
     5461 acgagaatgg cgaccatgat gggtgcaccc acgaactccg cggcggcgaa ccaccatccc
     5521 atcaggacaa cgagaacgac actgagttcg acgacgagat tggtcgacgc catctcgaat
     5581 gccatggccg cggtgaaatc cgcacccttg cgaaagatgg agcgcgcgag cgcgacagcc
     5641 gcgtaggagc acgacgacga tgcggcgccg agcgccagcg cgcgcagaat cgatttgggc
     5701 cgatcgtcag gcagaagcct gctcatctct tcgtgggaga cgacggcctg cacacaggcc
     5761 gagagcgcga atcccaggat cagcggccag aggatttccc atcccatggc cgcggccaat
     5821 tcgagcgcgt gagcgatggc tctcatcatc ggggtgcaca ttctgctaca cccgacgaga
     5881 tctccgacaa gcgctcgccc tcatgcgcca tgcagcagcg gccgcatttt aaacgaggtc
     5941 ggtggccaac agcggctcgg gagaaatgaa ttcgcccagc tcttgcacgg caacgatgag
     6001 caaatcgcga tcggcttgat tcacgcgcca accttccgaa agatccgaga acccccagcc
     6061 ggagggcgat cgccgtgccg ccgtaaagaa caaatggccg cggcacgcgc gcgagctcgg
     6121 gccacaggag gcgttgcggc tccggcagaa tcgaaaggtc agccgtgaac ggcatccact
     6181 cctcgctcgg gcccgatccg gcgcgccgcc ttcggtccgg gcgcaccgcc cagcccgagg
     6241 cgatgatgcc agaacgcctg cgatctcgtc gaaagaccgc cagggggcgg atcgcgcagc
     6301 acgtttacga aagcgctgcg cccgacctcg ttcaggagtg cgtgggcgtc ctcccaggtg
     6361 ccgatctcca tcacctgcgc gatgatgcgc gaacggttct cggaaacaaa tcggtcggga
     6421 gggctccacc acacatagcg gcgcgccagc tcgagcaatc gatcgcggtt cccactcacc
     6481 tggtaatcat agcgcagcgg caaactatgt gccacgtttg tctgttttct ttgttgcctg
     6541 cgcatgcgcc ggcgtgaaat cctttgtgcc gcggagggaa aatgcccttg aattttccca
     6601 aaggattcct ctggggcacg gcgacggccg cccatcaggt ggaaggtgga aacgtcgcga
     6661 gcgatctgtg gctcatggag aatgtaccgc agagcctgtt cgccgagccc tcgggcgacg
     6721 cctgcgatca ttatcatcgc tatccgcagg atattgccct gctgaaagga ttgggatttg
     6781 gcgcctatcg cttttcgatc gaatgggcgc ggatcgaacc ggaaccaggt ttctattccc
     6841 gcgcggcgct cgaccattat cggcgcatga tcgccgcatg ccgcgccatc ggcatcgtgc
     6901 ccatggttac ctttcatcat tttaccgcgc ccttgtggtt cacgcgcgac ggcggctgga
     6961 ctgagcccgg atccatcgac cactttgcgc gctattgcga gaccgcgggc cgcgcgctgg
     7021 gcgatctcat gctctatgcc tgcacaatca acgaggccaa cgttcccatg atgatcaccg
     7081 cgcagcgcga ggcggatcgc ggggcccgcg gcgagtcgcc ggagcgtttc gcgaagatcg
     7141 cgcggcgatg tggcggcgag gccaggcact gggcgccctt tcttcactgt gacggtcgcg
     7201 cggcgtcgct ccagctcatc gcggcccacc gcaagggcta cgaagcgcta aaatccgcag
     7261 ggctttcctg ccccgtcgga attaccctgg cgctgctcga catgcaggcc gaacgcggcg
     7321 gcgaagcaaa tcgcgacgcg gcggagcgtt tgattcaggg acagtttctc gaaagcacga
     7381 ggggcgacga tttcgtcggc gtgcagacct acacgcgaat ccgcttcgga ccgaaaggcc
     7441 atttgccgcc gcccagggac gcggagcgca cgcagatggg atacgaattc tatcccgaag
     7501 ctctcgatgc cacggtccgc acggcggcgc gcgtctcggg atgccccgtc ctcgtgaccg
     7561 agaacggcat cgggaccgaa gacgattccc gacgcattgc ttacgcaaaa cgcgccatcg
     7621 agggtttgca cgccgcgatc gcggatggtg tcgatgtccg gggctacttc cactggtcgc
     7681 tgctcgacaa ttacgagtgg ctcgaaggtt atcggccgaa attcggcctc gttgcggtcg
     7741 accgcaccac ccaggcgcgc aggcccaaac cgtccgggta ctggttgggg gaggtggcgc
     7801 gcaacaacgc gctcatctga cgtgcgtcat acaccctcga cggcgcggcg gagtccggag
     7861 atgtcgaagg gctttgccag aaacgccacg ccggcaaatg ccggctgcgc gcgcgcggcc
     7921 gaatggccgg tcgagatgat gatcttgagc tcgggacgca ggcctcgcgc ccggcgcaca
     7981 agctcctccc cgctcagatc cggcaggcca agatcggcga tcagcatgcc gatcgacggg
     8041 tcttcggcga gaagggtcag cccttcgtgc ccgttcccgg tgtcgatcgt ctccagcccc
     8101 atttccgcca gcatgtcgca gacggacatg cggatgaggg cttcatcctc cacgacgagc
     8161 gccttttcct tccgcgtctc agccaacctg gtcacctgcc gctgcctctg ggactgcacc
     8221 ctacaagaat ggctcgcgcc gaaccagaag ggatttccct gtctcgcgat tgcgttgaat
     8281 gcgctaatac ggaatcaagc atgcgacaaa ggggcagtca tgaaggattt tgcaggcaga
     8341 agcgccgtta tcaccggcgg cggcacgggc atggggcggc agctcgccct gcagcttgcc
     8401 gcggagggct gcagcgtcgc attgtgcgat gtttgcgccg agacgatggc ggagacaaag
     8461 cgcctcgccc tgtcgaatgc accccagggc acgcgggtct cgaccttcat tgccgatgtt
     8521 tcgatcgagg aacaggtgct cgccttcgcc gcgtccgtgg cgcgcgatct cgacaccg
//
LOCUS       DASZMC010000213         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5030030,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000213 DASZMC010000000
VERSION     DASZMC010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5030030"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /gene="raiA"
                     /locus_tag="VG274_04180"
     CDS             <1..391
                     /gene="raiA"
                     /locus_tag="VG274_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWE05881.1"
                     /translation="PPSQPGARSRSDGVDVHVEPMKVRVSGKQIEIGTALPDLVRQRV
                     EAALGKHFNGGAEANIVFSHEGFGYRADCTAHLDSGVVLKSQGEAVDAQHAFDAALDR
                     LEKQIRRYKRKLRNHHEKAKLPKDAGA"
     gene            411..890
                     /gene="ptsN"
                     /locus_tag="VG274_04185"
     CDS             411..890
                     /gene="ptsN"
                     /locus_tag="VG274_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HWE05882.1"
                     /translation="MEIVDLLAPEAVIAALKAQSKKQLLQELAQHAARITQIPERRIF
                     ETLIERERLGSTGMGQGIAIPHGRLAGLGRICGLFARLETPIAYDAVDDQPVDLVFLL
                     LAPEGAGADHLKALARVSRLLRNQATCEKLRAATRPEVLYALLTEPTNGGAHSSQAA"
     gene            complement(1096..1347)
                     /locus_tag="VG274_04190"
     CDS             complement(1096..1347)
                     /locus_tag="VG274_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HWE05883.1"
                     /translation="MSGYPDIDAAAAGAFGARIAYIKPTPAIEARRMGILPPGIRVPD
                     NVKLYVLHASDGSVLGFTDAYESAYGAAIENELTPLSVH"
     gene            complement(1349..1807)
                     /locus_tag="VG274_04195"
     CDS             complement(1349..1807)
                     /locus_tag="VG274_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HWE05884.1"
                     /translation="MNRNSAFGSPFLLGFDHLEQLIERTAKSAGESYPPCNIETLAAG
                     KLRITLAVAGFGGDELSVTLEDRQLIVRGRQSESGERTFMYRGIAARPFERVFVLAEG
                     IEVTGATLEHGMLFIELARPNLESVVRKIDIKCNADTKNAERDRKETRYG"
     gene            1967..>3514
                     /locus_tag="VG274_04200"
     CDS             1967..>3514
                     /locus_tag="VG274_04200"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005698.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HWE05885.1"
                     /translation="MSTPQIVLDDPRSGGERIYAGAFEVVRADAIGQVDAAFAKLDEA
                     LAAGRHAAGYFSYELGYALERRLAPLMPSSRNVPLLWFGIFADREFGGRCADESAPSA
                     HAGSLVHEWDEARHAAAFERIRALIAAGDLYQANLSYRARFAFVGAPYAFYRELRSRA
                     RARHCAFVDDGERQIVSISPELFFDISPAGAVRTRPMKGTAARGTTAEEDIQARARLA
                     ASEKERAENLMIVDLVRNDLSRIAIPGSVEVENLFEVETYPTVHQLVSTIAARLAPRM
                     GVNTVVRALFPCGSVTGAPKIRAMEVIREIETSPRGVYCGAIGYFAPDGSAAFNVAIR
                     TLTIAGGRGELGIGGAVVFDSRARAEYEECRVKARFYEAARHPLQLIETLRYEPGVGF
                     LRLGRHLARITSSAAALGFEFDAAGAGHELQRCVAGRFETLRVRLTLHEDGMMCAEAE
                     PFVATPVPWRFAVSPVRVSSRDPLLRHKTNRRVLHDSEHARLAASLGCDEVLFLNERG
                     ELTEGSRT"
BASE COUNT          606 a         1128 c         1134 g          646 t
ORIGIN      
        1 gcctccgtct caaccgggcg cgagatccag atcggacggg gttgacgtgc atgtggagcc
       61 catgaaggtt cgggtttcgg gcaagcagat cgaaatcggc acggcgctgc ccgatcttgt
      121 acggcagcgg gtcgaagcgg ctctcggcaa gcatttcaac ggcggcgccg aagccaatat
      181 cgtgttctcg catgagggtt tcggctaccg cgcggactgc accgcacatc tcgactcggg
      241 cgtcgtcctg aaatcgcagg gcgaggccgt cgatgcgcag catgcgttcg atgcggctct
      301 cgaccgtctg gagaaacaga ttcgccgtta caagcgcaag ctcagaaatc accacgaaaa
      361 ggcgaaactg cccaaagacg caggcgcctg accgcagcca ggaaggcatc atggaaattg
      421 tcgatctgct cgcgcccgaa gccgtgattg cggcgctgaa ggcgcagagc aagaagcagc
      481 tccttcagga gctggcccag catgccgcgc ggatcaccca gattcccgag cggcgaatct
      541 tcgaaaccct gatcgaacgc gagcgcctgg gcagcacggg catgggacag ggcatcgcca
      601 ttccgcatgg gcggcttgcg ggcctcggcc gcatctgcgg attgttcgca cggctggaga
      661 caccgatcgc ctacgacgcc gtcgacgacc agccggtcga tctcgtcttt ctgctgcttg
      721 cgccggaagg cgcgggcgcg gatcatctga aggcgctggc gcgcgtgtcg cggctcctgc
      781 gcaaccaggc cacctgcgag aagcttcgcg cggcgacgcg gcccgaagtt ctctatgcgc
      841 tgctcaccga acccaccaac ggcggcgccc actcgtcgca ggcggcgtaa taagtatagt
      901 gtctctaaaa cactaagcgg cacacttgtc ggcgagccaa aaaatcttaa cgcagtggtc
      961 gccgaggatg cgcagtgcgc gcagaaatca aatacgcgcg aagcgcgcag aaatcccctg
     1021 cgttcctctg cgcatcctcc gcggccgctg cgttaaatct gtcttcctgt cagaggagac
     1081 ccggaaccgc gcgcctcaat gcacgctcag cggcgtcagt tcgttctcga tggcggcgcc
     1141 gtaggcggat tcgtatgcgt cggtgaaacc gagaacgctt ccatcgctcg cgtgcagcac
     1201 atagagcttc acgttgtcgg ggaccctgat gcccggcggc aatattccca tgcgccgcgc
     1261 ttcgatcgca ggcgtgggtt tgatgtaggc gatacgtgcg ccgaacgcgc ctgccgccgc
     1321 cgcatcgatg tccggataac cgctcatgct acccataacg cgtctccttc cggtcacgtt
     1381 ccgcgttctt cgtgtccgcg ttgcatttga tgtcgatctt gcgcaccacg ctttcaaggt
     1441 ttggccgggc aagttcgatg aacaacattc cgtgttcgag cgtggcgccc gtcacttcga
     1501 ttccttccgc cagcacgaag acgcgctcga acgggcgcgc ggcaatgccg cgatacatga
     1561 acgtgcgctc gccgctctcc gactggcgcc cgcgtacgat cagctgccgg tcctcaagtg
     1621 tgacgctcag ctcgtcgccg ccgaatccgg ccaccgcgag cgtgatgcgc agcttgccgg
     1681 ccgccagagt ttcgatattg cagggcggat aggattcgcc tgcgcttttc gcagtgcgtt
     1741 cgataagctg ttcgagatgg tcgaagccca gaagaaacgg gctgccgaaa gctgaattgc
     1801 gattcatgtc caaagccctc cttgagcggc tttgccggct ccgccattgc ggccgccgat
     1861 tgtggcaggt ttgcggcccg caaacggcgc cgcgcctggg taggatgtgg ggagtgtgcg
     1921 gcacggatca agaacccgat gtgcctgcga ttccggagca tggccgatgt cgacgcccca
     1981 aatcgtactt gacgatccgc gaagcggcgg cgagcggatc tatgccggtg cgttcgaagt
     2041 ggttcgcgcc gacgcaatcg ggcaggtgga cgccgctttt gcgaagctcg acgaagcgct
     2101 cgctgcgggc cggcacgcgg caggatattt ctcgtatgaa ctcggttacg cgctcgagcg
     2161 gcgccttgct cctctcatgc cgtcctcccg caacgttccg ctgttgtggt tcggtatctt
     2221 cgccgaccgg gagtttggcg gccgatgcgc ggacgagtct gcgccgagcg cccatgcggg
     2281 aagccttgtg catgaatggg acgaagcgcg ccacgccgcg gcattcgaac gcatccgcgc
     2341 gctcatcgcc gccggcgatc tctatcaggc caatctgagc tatcgcgcgc gattcgcgtt
     2401 tgtgggcgcg ccgtatgcgt tttatcgcga actccgcagt cgcgcgcgtg cacgtcattg
     2461 cgccttcgtc gacgatggcg agcggcagat cgtgagcatc tctccggagc tgttcttcga
     2521 catttcgccc gcgggcgcag tgcggacgcg gccgatgaag ggcacggctg cgcgcggcac
     2581 gaccgcggag gaggacatcc aggcgcgcgc gcggcttgcg gcgagcgaga aggagcgcgc
     2641 cgagaatctg atgatcgtcg atcttgtgcg caacgatctc tcccgcatcg caattccggg
     2701 aagcgtcgaa gtggagaatc ttttcgaagt ggaaacttat ccgaccgtcc accagctcgt
     2761 gtcgaccatc gcggcgcgcc ttgcgccgcg gatgggcgtg aacaccgttg tgcgggcgct
     2821 ttttccctgc ggctccgtca ccggcgcgcc caagattcgc gccatggagg tgatccgcga
     2881 aatcgagacg agcccgcgcg gcgtctattg cggcgccatc ggatatttcg cgcccgacgg
     2941 ctcggccgcc ttcaacgtcg ccatccgtac actgacgatt gccggcggac gtggcgagct
     3001 cggcatcggc ggcgccgtcg tattcgattc gcgggcgcgg gccgaatatg aggagtgccg
     3061 cgtcaaggcg cgcttctacg aggcggcgcg ccatcctttg cagctgatcg aaaccctgcg
     3121 ctacgaaccg ggcgtgggat ttctccgcct cggccggcat ctcgcgcgca tcacgtcgtc
     3181 ggctgcggcg ctcggtttcg agttcgacgc ggcgggcgcc gggcacgaat tgcaacgatg
     3241 cgtagcgggc cggttcgaaa ccctgcgcgt gcgcctcacg cttcatgagg acggcatgat
     3301 gtgcgccgag gccgaaccgt tcgttgctac tccggtgccg tggcgattcg cggtttcgcc
     3361 cgtgcgcgtg tcgagccgcg accccctcct gcgccacaag acaaaccggc gcgtgcttca
     3421 cgacagcgaa catgcacggc tcgccgcgtc gctgggttgc gacgaggttc tcttcctcaa
     3481 cgagcgcggc gaactgaccg aaggcagccg cacg
//
LOCUS       DASZMC010000214         5698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1209226,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000214 DASZMC010000000
VERSION     DASZMC010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209226"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /gene="argS"
                     /locus_tag="VG274_04205"
     CDS             complement(<1..1585)
                     /gene="argS"
                     /locus_tag="VG274_04205"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWE05886.1"
                     /translation="MTPLTAELSAIAGRAFATEGFDSALGAVRASDRGDLAQFQCNGA
                     MAAGKLAKSPPRAIADRIAARLRSEELFGKVEVAGPGFLNLDVTDDALARRVAAITAE
                     ERLGAPDTGRNKTLVIDFGGPNIAKPMHVGHLRTSIIGDSLQRLFRANGWRVLSDVHL
                     GDWGLPMGQLIAEIGRRGIAPIYFDPKFTGPYPNDSPVTMEQLEELYPAAASACKSDP
                     ARLEEARAATAELQAGRPGYRALWRHFFDVSERGLKREYASLGVVFDLWNGEADVDPL
                     IAPMIEDLKARHLAELSEGALIIPVSEPGDRKEIPPLILLKSDGAVLYGTTDLATIVD
                     RVRSHDPDLILYVVDQRQHMHFEQVFRAARKGGLNGRAELEHAGFGTVNGPDGKPFRT
                     RAGGVMKLYELIAMATEEAALRLQEQGLAAEYPPEERAEIAKAVGLAAIKFADLSNFR
                     TSDYIFDLARFTRFEGKTGPYLQYAAVRIQSILRKAEAEGYARAAPAVRSAEERRLLL
                     VLASLTDAMANGETRRAPNV"
     gene            complement(1582..2118)
                     /locus_tag="VG274_04210"
     CDS             complement(1582..2118)
                     /locus_tag="VG274_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05887.1"
                     /translation="MHISNLRRVLAILVGLVAAGCSSDKTGDCPTITGITDAAIQTVF
                     RPGTTQDPSNVLYTAEIIGVTGTCDIDTKEHTADSRLDVSFRVTRAPSGGAAHYTIPY
                     FVAVTVGSERILARRNYSVAIVFEPGQTTATASEHVESATLHTDKDKQPYDYQVLVGL
                     QLTREQLKYNRQSGHYGS"
     gene            2435..3394
                     /gene="thrB"
                     /locus_tag="VG274_04215"
     CDS             2435..3394
                     /gene="thrB"
                     /locus_tag="VG274_04215"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWE05888.1"
                     /translation="MAVYTDIAFDDLGIMLAEYDIGAPRRFKGIAEGIENSNFLLQTD
                     RATYILTLYEKRVSAKDLPFFLELLEHLAAHGIACPLPVRSRNGAQSTTIKGRPAALL
                     TFLDGLSLSRPRADHCAAASTALAALHRAGADFARTRSNNFSLHGWRRLADAILPRAD
                     RAEKGLAQLIETNLAAVEEMWPSDLPSGVIHADLFPDNVLFLHDRISGLIDFYFACHD
                     FLAYDVAVMLNAWCFDSDGSFNRTKSRSVISAYANARPLTPCETAALPVLARGAALRF
                     LLTRLQDWLDRDPRMLARVKDPLEFANYLRFHAKVVSPAEYGA"
     gene            3391..3840
                     /gene="rnhA"
                     /locus_tag="VG274_04220"
     CDS             3391..3840
                     /gene="rnhA"
                     /locus_tag="VG274_04220"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HWE05889.1"
                     /translation="MNAPIVDIFTDGACSGNPGPGGWAAILRSGRREKAISGGERATT
                     NNRMEMTAAIRGLETLKLPSTVTIYTDSRYLMDGATLWMKRWKLNGWRTADKKAVKND
                     DLWRALDAAGARHHLTWKWVAGHAGHPENERADALARAAIPSAIGRS"
     gene            complement(3835..4215)
                     /locus_tag="VG274_04225"
                     /pseudo
     CDS             complement(3835..4215)
                     /locus_tag="VG274_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
     gene            complement(4299..4781)
                     /locus_tag="VG274_04230"
     CDS             complement(4299..4781)
                     /locus_tag="VG274_04230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWE05890.1"
                     /translation="MTIKPGDRIPSATLMQMKGGAPQPVKTDDLFRGKKIAIFALPGA
                     FTPTCSAKHLPGYLQHADDLRNKGVDAIACVSVNDAFVMGAWGESQNAGDKVMMLADG
                     NGDFTRALGLEMDATKYGMGKRSQRFSMLVDDGVLKTLNVEEPGAFSVSSAEHMLQKL
                     "
     gene            4923..5516
                     /locus_tag="VG274_04235"
     CDS             4923..5516
                     /locus_tag="VG274_04235"
                     /inference="COORDINATES: protein motif:HMM:NF001268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HWE05891.1"
                     /translation="MASTKESRATSGENFLEGKLLIAMPGMTDPHFEKSVIFMCAHSA
                     EGAMGIMINKPVAGLSFHDLIGKLELKVTPETPNFPVLYGGPVETGRGFVLHSGDYES
                     SEATLPVSENVSLTATLDILRAMADGRGPHHAIFALGYAGWQAGQIEDEIRRNGWIHC
                     DSDPEILFDHELNAKWATALRKLGINASGLSAHTGHA"
     gene            complement(5484..>5698)
                     /locus_tag="VG274_04240"
     CDS             complement(5484..>5698)
                     /locus_tag="VG274_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05892.1"
                     /translation="LGRVVVLEGVETERDIQLLREIGCEYAQGFYFGDSLARVDVPVF
                     VAAHRPAANMGSAEFPSGVSGVSGKA"
BASE COUNT         1128 a         1809 c         1662 g         1099 t
ORIGIN      
        1 gcacattcgg cgcacgccgg gtttccccat ttgccatcgc gtcggtcaaa gaggcgagca
       61 cgagcagcaa tcggcgctct tccgccgagc gtaccgccgg cgccgcgcga gcgtaacctt
      121 cagcctccgc cttacgcaga atcgactgaa tccggacggc tgcgtattgc agatagggcc
      181 cggtcttgcc ctcgaaccgg gtgaagcgcg ccagatcgaa aatgtagtcg gacgttcgaa
      241 aattcgagag gtccgcaaac ttgatggccg caagcccaac agctttggcg atctccgcac
      301 gctcctcggg cggatattcg gcggcgagcc cctgttcctg aaggcgcagt gcggcttcct
      361 cagtcgccat ggcgatcagc tcgtagagct tcatcacgcc gccggcgcgt gtcctgaacg
      421 gcttgccgtc aggaccattc acggtgccga aaccggcatg ttcgagttcc gccctgccat
      481 tcagaccacc tttgcgtgcc gcccggaaca cctgctcgaa atgcatgtgc tgccgctggt
      541 ccaccacgta gagaatgaga tccggatcgt gcgagcgcac acgatcgacg atggtagcca
      601 gatccgtagt cccgtagagc accgcgccat cggatttcag cagaattagc ggcggtattt
      661 ctttccggtc gccaggctcg ctcaccggaa tgatgagcgc gccctcgctc agctccgcga
      721 gatggcgcgc cttgagatct tcgatcattg gcgcaatcag cggatcgacg tcggcctcgc
      781 cgttccagag atcgaatacg accccgaggc ttgcatattc gcgcttcaga cctcgttcgg
      841 agacatcaaa gaagtggcgc cacaatgcgc ggtagcctgg ccgccccgcc tgcagctctg
      901 ccgtcgccgc acgcgcctct tcgaggcgcg caggatcgga tttgcaggct gaggctgccg
      961 cgggataaag ctcttcgagc tgttccatcg tgacgggcga atcgttcgga tagggccctg
     1021 tgaatttcgg atcgaagtag atgggcgcaa tgcctcttcg cccgatttcg gcgatgagct
     1081 gtcccatcgg caaaccccag tcgccgagat gcacgtcgct cagtacgcgc cagccattgg
     1141 cgcggaaaag acgctgcagg gaatcgccaa tgatcgacgt tcgcagatgg cccacatgca
     1201 tgggcttggc gatattgggt ccgccaaaat cgatcacgag agtcttgttc cggcccgtgt
     1261 ctggtgcgcc gagccgctcc tccgccgtaa tcgcagccac acgacgtgca agcgcatcgt
     1321 ccgtcacgtc aaggttgagg aatcccggtc cggcgacttc aactttccca aaaagctctt
     1381 cactgcgcag acgcgcggca atgcgatcgg cgatggcacg cggaggtgac tttgcgagct
     1441 tccccgcggc catcgcgcca ttgcactgaa actgcgccag atctccacga tccgaggccc
     1501 gcaccgcgcc aagcgccgaa tcgaatcctt ccgtggcgaa cgcccgcccc gcgatcgccg
     1561 acaattccgc cgtcagcggc gtcatgatcc gtagtgcccg ctttgccggt tatacttgag
     1621 ctgctccctg gtgagctgca agccgacgag cacttgataa tcgtagggct gcttgtcctt
     1681 gtcggtgtgc aaggttgccg actcgacgtg ttcgctcgcg gtcgccgtcg tctggcctgg
     1741 ttcgaagacg atggcgacgg aataattccg ccgcgccaga atacgttcgc tgccaacggt
     1801 aacggcaacg aagtatggaa tggtgtaatg tgccgctccg ccgctcggcg cacgcgtgac
     1861 acggaaggag acgtccagtc tggagtccgc ggtgtgttcc ttcgtgtcaa tgtcgcaagt
     1921 gccggtgaca ccgatgatct ccgccgtgta tagcacgttc gaaggatctt gcgtggtccc
     1981 ggggcgaaac accgtctgga ttgccgcatc ggtgatgccg gtgatcgtgg ggcagtcgcc
     2041 ggtcttgtcg ctcgagcagc ccgcggcgac cagtccaaca agaattgcca aaacacggcg
     2101 aaggttggag atgtgcatgt gaacagcgat caatctggat tgcgagggcg gtcgcgcaaa
     2161 taatggtcca tggacttttc gacggcaccc cacgaacggg atttcgatgc cgccgtttcg
     2221 cccgcgaagg agatgccctg agccgtagtc aaccccgtac ctctgcttga catttccgat
     2281 gccgaagtcg acttctcggt cgagctttcc gaaaacagcg cggcaatcta gcgaagcaaa
     2341 tcgaacatgc aaaggcgaaa ccgcgacggc gcccgtttga gttccgaacg cccaaacatc
     2401 cgcctgacgc gcagcttgcg gatttgaggc cgccatggcc gtctatacgg acatcgcgtt
     2461 cgacgacctt ggtatcatgc tcgccgaata cgacatcggg gcgccccgac gcttcaaggg
     2521 catcgccgaa ggtatcgaga attccaattt tctgctgcag accgaccgcg ccacctacat
     2581 tctcacgctg tacgagaaac gtgtcagcgc aaaggatctc cctttttttc tggaactgct
     2641 cgagcatctg gccgcgcacg gcatcgcctg tcccctgccc gtgcgttcgc gcaacggtgc
     2701 gcaatcgacg acgatcaaag gtcgaccggc cgctcttctg acgttcctcg acggattgtc
     2761 gctcagccgc ccacgtgcag atcactgcgc agccgcgagc acggctctgg ccgccttgca
     2821 ccgcgcgggc gccgatttcg cacggacgcg cagcaataat ttctccctcc acggatggcg
     2881 tcggctcgcg gacgccattc ttccgcgtgc cgacagggct gaaaagggac tcgcgcaact
     2941 gatcgaaacc aatcttgcgg cggtggaaga aatgtggccg agcgatctac cctccggcgt
     3001 catccacgcc gatctgtttc cggacaatgt gctgttcctt catgacagga tttcgggcct
     3061 gattgatttc tatttcgcct gccatgattt ccttgcctac gatgtcgcag taatgctcaa
     3121 cgcctggtgc ttcgattccg acggcagctt caaccgcacg aaatcccgga gcgtgatctc
     3181 ggcgtacgca aacgcgcgcc ctcttacgcc ctgcgagacc gcggcacttc ccgtcctggc
     3241 gcggggtgcg gcattgcgat tcctgctcac ccgcctgcaa gactggctcg atcgcgaccc
     3301 gcgaatgctt gcgcgcgtaa aggatccgct cgaattcgca aattacctgc gctttcacgc
     3361 caaggttgtt tcgccggcgg agtatggtgc gtgaacgccc caatcgtgga tatcttcacg
     3421 gacggcgcgt gctcgggaaa tcccggtccg ggcggctggg ccgcgatctt gcgctcggga
     3481 cggcgcgaga aggcgatttc gggcggcgaa cgagcaacga ccaacaaccg catggaaatg
     3541 accgccgcga tccgggggct cgagacgctc aagctgccgt ctaccgtcac catctatacg
     3601 gattcgcgct atctgatgga tggtgcgacg ctatggatga agcgctggaa actgaacggc
     3661 tggaggaccg ccgacaagaa ggcggtcaaa aacgacgatc tttggcgtgc actcgatgcc
     3721 gcaggtgcac gccatcacct tacctggaaa tgggtagccg gccatgccgg ccatcctgaa
     3781 aacgagcgag ccgacgccct cgcgcgcgcc gcaattccat ctgcgatcgg acggtcatga
     3841 tgcggaagtg ttggccgcat gttcgtcgat caaagcgccg tcgcggccgg cgatataggc
     3901 tggagtgccg ggcgccaatt tgacccatgg gtagcgcgag cgttcgaaaa ccgagcgcgc
     3961 gggtcccgga aagtccaaac tcgcgaacgc gcccatggca atcgcgattc tgccgggatg
     4021 ccgttcggtt tcccaccata ccgtcgaacc gcaagttggg cagaagtgaa acgcaagccg
     4081 cctgccctag tcgctgacgc gtgcgtagat ccggctcgga tcggatacac gcaccgcatt
     4141 cgcctcgaaa aacgcgctcg cgccgaacgc agaaccggtg cgccgctgac attcgaggca
     4201 gtggcaaacg accacgacaa ccggctcgcc ctttcgtttc cacagtgagc gcgccgcagc
     4261 aacaacaggc cgttcttgtc aatttttcag tgcctgactc agagtttctg aagcatgtgc
     4321 tcggcgctgg agaccgaaaa cgcgccaggc tcctcgacat taagcgtctt taacacgccg
     4381 tcgtcgacaa gcatcgaaaa cctctggctg cgctttccca ttccgtattt tgtggcgtcc
     4441 atttcgaggc cgagggcgcg ggtgaagtcg ccattgccgt ccgccagcat catgaccttg
     4501 tcgccagcgt tctggctttc gccccaggcg cccatgacga acgcgtcatt gaccgacacg
     4561 caggcaatcg cgtcgacgcc tttgttccgc aggtcgtctg catgctgcaa ataaccgggc
     4621 agatgctttg ccgaacaggt cggcgtgaaa gcacccggaa gtgcgaagat cgcaatcttc
     4681 ttgccgcgaa acagatcatc ggtcttcacc ggttgcggtg cacctccctt catctgcatc
     4741 agcgtcgcgg atggaatcct gtcgcctggc ttgatcgtca tgcttcgtct ccctgttgcc
     4801 ggtgggcgcc gcttttagcc caccctttgt gcaaggcaaa gggcatgaag gccacacaac
     4861 cccggccccg gatatgcatg gccggttgtg gcacgcggct cccggcgtgg taagctcgca
     4921 tcatggcaag cacgaaagag tcgcgcgcaa caagcggcga aaactttctc gaaggcaagc
     4981 tcctgatcgc aatgccgggc atgacggatc cgcatttcga aaagagcgtg atcttcatgt
     5041 gcgcccactc cgccgaaggc gcgatgggaa tcatgatcaa caaacccgtt gccggactgt
     5101 cctttcacga cctgatcggc aaactcgaac tgaaagtcac tccggagacg cccaactttc
     5161 cggtcctcta tggcggaccg gtggaaacgg gacgcggatt cgtcctccac agcggcgact
     5221 acgaaagctc cgaagccact ctacctgttt cggagaatgt atcgctcact gcgacattgg
     5281 acattctgag ggcgatggcg gatggccgcg gaccccatca tgccatattc gcgcttgggt
     5341 acgccggctg gcaagctggc cagatcgagg acgagattcg ccgcaatggc tggatccatt
     5401 gcgacagcga tcccgaaatt ctgttcgatc acgagctgaa tgcgaaatgg gcgacggcgc
     5461 tgcgcaagct cggcatcaac gcttcaggcc tttccgctca caccggacac gcctgacggg
     5521 aactccgcgg aacccatgtt tgcagcggga cgatgcgcgg cgacgaatac gggcacatcg
     5581 acgcgcgcaa gcgaatcgcc aaagtagaag ccctgcgcat attcgcagcc gatttccctc
     5641 agcagctgaa tatccctttc cgtctccacg ccctccaaca cgacgacgcg ccccaatt
//
LOCUS       DASZMC010000215         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2567800,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000215 DASZMC010000000
VERSION     DASZMC010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567800"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VG274_04245"
     CDS             <1..542
                     /locus_tag="VG274_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWE05893.1"
                     /translation="LLEREEIAFARFDGSLGGADKDAAIADFRDCVPVLLSTESGGEG
                     RNIQFCNTLINFDVPWNPMAIEQRIGRIDRIGQAREVFVFNLVSRGTLEEQVLALLDE
                     KISMFELVVGEAGAILGSIEEEKDFSELVLDAWLETTEDARAGAFDDIGRRLVEARGQ
                     HESAKALDEALFGEDFETA"
     gene            551..1723
                     /locus_tag="VG274_04250"
     CDS             551..1723
                     /locus_tag="VG274_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05894.1"
                     /translation="MGGLREFVGGIVERHGAVVEMCEPDGLDVLSPRPLQDVMGWREL
                     ERLDFGAERPQSHAEGLHRIIRIGLEGDWLGRFGALLGDRGHWTERQLALGDAPPAPS
                     DPQRMLDRALDLSNAVWRLQGHRAAIARCLVLAFRYAAVSDEKREGLIWLGFNQDTGA
                     AIDSIAARLLSGRLRESAWQAPDAVARGAAGPRWDSALLEARLRPLLDHRVRQEIDPF
                     LRAMQRRLERDRARVHAFHDDLWISSQKRLSALANAPGERADADRRREQLRAAAIERE
                     YGSKLGDLRHNYALRVTLEWVQALELYLPVERFDLLVRRRKGERRILLDWHPLVRMAE
                     PPCCDWGLGLDSVRLVCDEKLHLTEPAGQAPCAACGKGWCRACHPAACSRCAKAGG"
     gene            1856..>2780
                     /locus_tag="VG274_04255"
     CDS             1856..>2780
                     /locus_tag="VG274_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWE05895.1"
                     /translation="MNIFVTGASGFVGGAFVKHVAGRHAITAMSRSEASDAKIRAAGA
                     GPVRCSLDDVSAAHVKGAEAVVHCAAHVEDWGPWREYWRFNVEGTKRMLAVAKDAGVR
                     RFVHIGTEAALLHGQDLRDADETYPLALHSPFPYSRTKAHAEKLVREANRPGFETIVV
                     RPRFVWGPGDQTILPAIREMAARGQFAWIGGGRNRTSTSYIGNLSYAIELALTKGRSG
                     EAYFVLDGPPVEFRDFLTRMAQATGIVLPDREIPGWLARTIGYVGETAWRTLPLKGRP
                     PLTRFAANIMSRDCILVDAKARREMGYAPPFT"
BASE COUNT          454 a          861 c          967 g          498 t
ORIGIN      
        1 agctgctgga acgcgaggaa attgcctttg cgcgctttga cggcagcctt ggcggagccg
       61 acaaggatgc ggccatcgcc gatttccggg attgcgtccc ggtgctgctt tccacggagt
      121 cgggcggcga gggccgcaac atccagtttt gcaacacact gatcaatttc gacgttccat
      181 ggaatccgat ggcgatcgag cagcgcatag gccgcatcga ccgcatcggt caggcgcgcg
      241 aagtattcgt cttcaatctc gtgagccgcg gcacgctcga ggagcaggtg ctcgcgcttc
      301 tcgacgagaa gatttccatg ttcgagctgg tggtcggaga agccggcgcg attctcggca
      361 gcatcgagga agaaaaggac ttctccgaac tcgtgctcga tgcctggctg gagaccaccg
      421 aagatgcgcg tgccggcgcc ttcgacgaca tcggacgccg cctcgtcgag gcgcgcgggc
      481 agcatgaaag cgccaaggcg ctggacgaag cgctgttcgg cgaggacttc gaaaccgcgt
      541 aagggcgacg atgggcggat tgcgggaatt cgtaggcgga attgtggagc gccatggcgc
      601 agtcgtggag atgtgcgagc cggacggact cgacgtgctt tcgcctcgtc cgctgcagga
      661 cgtcatgggc tggcgggagc tggaacggct cgacttcggg gcggaacgcc cgcaatctca
      721 tgcggagggt ctgcaccgca tcatccgcat cgggcttgag ggcgattggc tcgggaggtt
      781 tggtgcgctg ctcggcgatc ggggacattg gaccgaacgc cagctcgctc ttggagatgc
      841 gccgccggcg ccaagcgacc cccagcgcat gttggaccgc gcgctcgatt tgtccaatgc
      901 ggtctggcgc cttcaaggcc atcgggcggc aatcgcgcgc tgcctggttc tggctttccg
      961 ctacgccgcc gtgtcggacg agaagcgcga aggactgatc tggctcgggt tcaaccagga
     1021 tacgggcgcg gcgatcgatt ccatcgcggc gcgtttgctg tcaggccggc tgcgcgaaag
     1081 cgcgtggcaa gcgcccgacg cggtggcgcg cggagcggcc gggccacgct gggattctgc
     1141 cctgctggag gcgcgcctgc gtccgcttct cgatcatcgc gtgcggcagg agatcgatcc
     1201 tttcttgcgc gcgatgcaac gccggctcga gcgcgaccgt gcccgcgtgc atgcttttca
     1261 cgacgatctc tggatatcgt cccagaagcg gctctccgca cttgccaacg ctccggggga
     1321 aagggcagat gccgaccggc gccgcgagca attgcgtgcc gccgccatcg agcgcgaata
     1381 cgggagcaag cttggcgacc ttcggcacaa ttacgcgctg cgcgtgacgc tcgaatgggt
     1441 gcaggcactg gaactgtatc tgccggtcga gcgcttcgat ctgctcgtcc gccgccgcaa
     1501 gggagagcgg cgaatcctgc tcgactggca cccgctcgtc cggatggcgg aaccaccctg
     1561 ctgcgattgg gggctggggc tcgattccgt gcggctcgtc tgcgacgaga agctccacct
     1621 gaccgagccg gccggacagg cgccttgcgc ggcctgcggc aagggctggt gccgcgcctg
     1681 ccatccggcc gcgtgctcgc gctgcgcgaa ggcgggtggt tgacgttcgg ttgggatggc
     1741 gtcgataccg actacgatct cgagtttctg gcccggacaa tggagcgcga actggcgcac
     1801 atcccgcaga tgaaggcggc ttgagttatg ccgcccatga cgcggtgaga tacgtatgaa
     1861 catcttcgtc accggagcat cgggtttcgt cggcggcgcc ttcgtgaagc acgtagccgg
     1921 tcgccatgcg atcacggcga tgtcgcgttc cgaggcaagc gacgcgaaaa ttcgcgcggc
     1981 gggcgccggg cccgtgcggt gctcgctcga cgatgtttct gcggcgcatg tgaagggagc
     2041 ggaggcggtc gtccattgcg cggcgcatgt cgaggattgg gggccgtggc gggagtattg
     2101 gcgcttcaac gtcgaaggca cgaagcggat gctcgccgtg gcgaaggacg cgggcgtcag
     2161 gcgcttcgtg catatcggca ccgaagccgc gctgcttcac gggcaggatt tgcgcgatgc
     2221 ggacgagacc tacccgctcg cccttcattc gcccttccct tattcgcgca ccaaagccca
     2281 tgccgagaaa ttggtgcgcg aagccaatag gcccggcttc gaaacgatcg tcgtgcggcc
     2341 gcgcttcgtg tggggacctg gcgaccagac gatcttgccc gcgatccgcg agatggcggc
     2401 gcgcggccag ttcgcctgga tcggcggcgg gcgcaaccgg acctcgacga gctatatcgg
     2461 caatctttcc tacgcgatcg agcttgcctt gacgaagggg cgctccggcg aagcctattt
     2521 cgtgctcgac ggaccgccgg tggaattccg cgattttctc acccgcatgg cgcaggcgac
     2581 ggggatcgtt ttgcccgatc gcgaaattcc cggctggctt gcgcgcacca tcggttatgt
     2641 gggcgagact gcctggcgca cgctgccgct aaagggcaga ccgccgctca cccgcttcgc
     2701 cgccaacatc atgtcgcgcg attgcatcct ggtcgacgcg aaggcccgcc gcgagatggg
     2761 ctacgcgccg ccttttacga
//
LOCUS       DASZMC010000216         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_85066,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000216 DASZMC010000000
VERSION     DASZMC010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85066"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VG274_04260"
     CDS             <1..1056
                     /locus_tag="VG274_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HWE05896.1"
                     /translation="KQKAVLYVNSDTNHRGTLSVEGSQDFEGLADAVSRSVIDPEKNV
                     SVGERMRAHLRLASFDSHGEDKDHVKAVAAFAADPARDLPIDAIGSGSDYGSFVNHLG
                     IPVLNLGYSDEGKSSGVYHSRYDTFEHHSRFVDPGFVYDSLLAKTIGRAVLMAADSDL
                     PLQRPSDFAAEIAHDLKEVKKLAEDGRDKARKQAAMLRDNAFTLDADPTKPFGAPTTL
                     KPVPVFDFSPLDLAVARLTKSAKTYDDALAQHGAALSASGLAQLQSLMQNIDQTLTAD
                     VGLPGRPWFRNLIYAPGTLTGYGVKTLPGVREGIEQERFDDAVRYTTLTAGVLNAYCD
                     RLDQASALLRETQQAAR"
     gene            complement(1306..1698)
                     /locus_tag="VG274_04265"
     CDS             complement(1306..1698)
                     /locus_tag="VG274_04265"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWE05897.1"
                     /translation="MDRVTGGCLCGKIRIVASGLPYRVGLCHCLDCRKHHGALFHASA
                     VFPQDAVTIEGETRDYAGRFFCPRCGSSVFGRSADEIEVNLGSLDAPDQLVPTYESWI
                     VRRESWLPPFPLTRRYDRDRDATGRLEE"
     gene            complement(1947..>2627)
                     /locus_tag="VG274_04270"
     CDS             complement(1947..>2627)
                     /locus_tag="VG274_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWE05898.1"
                     /translation="LMAAGRVRSATLLANGRAFDEAVSRIGDFPHCSFGVHLNVATRM
                     PVGPASELAPILGADGQFRVAAVRRLFVPQPVRNAVLREWISQVAKVMSAGVAVSHID
                     SHHHVHIIPGLFGTLKRLQRECGISRVRLAQTRRGDRSLAAVPFRLGRAIWNRALRMD
                     GTRTTDDFCSLSTFRTLSKETYAGSRTIEIMVHPGSAARDEENRLLASEWWTHVMRDN
                     EMTSYNDL"
BASE COUNT          514 a          862 c          801 g          450 t
ORIGIN      
        1 aagcagaagg ccgtgcttta cgtcaactcc gacaccaatc atcgcggcac tctttcggtt
       61 gaaggcagcc aggatttcga ggggctggcc gatgcggttt ccaggagcgt gatcgatccc
      121 gagaagaacg tttccgtggg cgagcgcatg cgcgcccatt tgcggctcgc ctccttcgat
      181 tcccacggcg aggacaagga tcacgtgaag gccgtggcgg cgtttgcggc cgatcccgcg
      241 cgcgatctgc ccatcgacgc aataggctcg ggctccgact atggcagctt cgtcaaccat
      301 ctgggcattc ccgtgctcaa tctcggatat tccgacgagg gcaagtcgag cggcgtctac
      361 cattcgcgct acgacacgtt cgagcatcac agccgcttcg tcgatccggg cttcgtctac
      421 gattcgctcc tggccaaaac gatcgggcgc gccgtgctca tggcggccga ttccgatctg
      481 ccgctgcaac gcccttccga cttcgcggcc gagatcgcgc acgatctcaa ggaggtgaag
      541 aagctcgccg aggacgggcg cgacaaggcc cggaaacagg cggccatgct ccgcgataat
      601 gcgttcacgc tcgacgccga tccgacgaag cccttcggcg cgcccaccac gctcaagcct
      661 gtaccggtct tcgatttctc cccgctggat ctcgccgtcg cgcgactaac aaagagcgcg
      721 aagacatatg acgacgcgct cgctcagcac ggcgccgcac tttccgccag cggactggcg
      781 cagcttcagt ctctgatgca gaacatcgat cagacgctga ccgccgatgt ggggctgccg
      841 gggcggccat ggttccgaaa cctcatctac gcgccgggaa cgctgacggg ctacggcgtg
      901 aagacgctgc ccggcgtgcg cgagggcatc gagcaggagc gcttcgacga tgccgtgcgc
      961 tacaccacgc tcacggccgg cgtgctcaac gcctattgcg acaggctgga ccaggcgagc
     1021 gcccttctcc gcgaaacgca acaggcggcg cgctagcgga cccaatctga cacttcgtac
     1081 cctaaaattg tcatggcccg gtttaaccgg gccacccagc cggcgcgcgt ctacgcgcgg
     1141 aacacaatgc tctgagcggt ctcgaccggg tggcccgctt gcgcgggcca tgacattttt
     1201 ttgggtgcaa agtgttggaa tcgcatcgct agcggtccag ttccgacaga tgaatcccaa
     1261 aaaaccttgt catcgcccgc caccccgatc cgcgcgagag cgcacctact cctccaagcg
     1321 gcccgtggcg tcacgatcgc ggtcgtatcg tctggtgagc ggaaacggcg gcaaccagga
     1381 ctcgcgacgg acgatccagc tttcgtaggt tggcaccagc tggtcggggg catccaggga
     1441 tcccaaattc acttcgattt cgtcggcgct gcgtccaaaa acggacgagc cgcagcgggg
     1501 acagaaaaac cgtccggcgt agtcgcgtgt ttcgccctcg atcgtcaccg catcctgggg
     1561 gaacaccgcg gaagcgtgaa aaagggcccc gtgatgcttg cggcagtcga gacagtgaca
     1621 aaggccgacc cgatatggca gtcccgacgc cacaattcgg atcttgccgc acaggcaacc
     1681 gcccgtgact cgatccatgg ctcgcgcctc ctctacaatc aagcggtcgg aatgcttacg
     1741 acgaaccaag tcgagcgtcg atgcgccgcc cgcaagcgca ggcaggcggc ggcgttgacg
     1801 gccatggcgt aaattcgaaa accacaccgc agccgccggc gccgctgcac gagacaccgc
     1861 cgccctgata ttccgctcgc gccagcgaat tcccgggaat caatcattcg caattgcctg
     1921 tccggagcgg ccaagggggc gggcgctcat agatcattgt agctggtcat ctcgttgtcg
     1981 cgcatgacgt gtgtccacca ttccgaagca agcagtctgt tttcctcgtc ccgggcagcc
     2041 gatccgggat ggaccatgat ctcgatcgtt cgcgagcccg cgtacgtttc tttggacagt
     2101 gttctgaacg tcgaaagact gcaaaaatcg tcggtcgtcc ttgttccgtc catgcgcagc
     2161 gcgcggttcc agatcgccct gccaagacga aaaggaaccg ccgcgagcga acggtctcca
     2221 cgccgggttt gcgccaggcg cacgcgggag attccgcatt ccctctgcag gcgcttgagc
     2281 gtgccaaaca gacccggaat gatgtgcaca tggtgatgcg aatcgatatg agacacggcg
     2341 acgccggcgc tcatcacctt ggcgacctgg ctgatccact cgcgcaatac cgcgttgcgt
     2401 acaggctgcg ggacgaacag cctgcggacg gctgccaccc gaaactgccc gtcggcgccc
     2461 agaatcggcg cgagttcgga cgcgggcccg acgggcatgc gcgtggcgac gttgagatga
     2521 acgccgaagg agcaatgggg aaaatccccg atccttgaaa cggcttcgtc gaaggcgcgg
     2581 ccgttcgcca acagcgtcgc ggagcgaacc cggcctgcgg ccatcag
//
LOCUS       DASZMC010000217         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2290286,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000217 DASZMC010000000
VERSION     DASZMC010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2290286"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..907
                     /locus_tag="VG274_04275"
     CDS             416..907
                     /locus_tag="VG274_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWE05899.1"
                     /translation="MDEAELERSFEAHAAALVLYARQWLDRSGAEDAVQEAFVRLLLQ
                     PTPPRQVKAWLYRAVRNEAITQWRSARSRGRRERRAALPETLFDYDTTAAGDVEALTT
                     AIARLPPTQREILVLRVWGELTLAEISELIEAPVSTVFQNYRTALGTMRKWLEEPCQK
                     KTH"
     gene            886..1365
                     /locus_tag="VG274_04280"
     CDS             886..1365
                     /locus_tag="VG274_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05900.1"
                     /translation="MPEEDALNTEERETLAAVARFKPATIRLNPFRIRSEAQRRRAKR
                     QVWLWRGIAAALAAGLIVSWIWRPGSAIREHVVYVRLPAPAIAPETALPRSATTEIEP
                     RMTPPLGDDYLAVRDRVLAFGIDALRPHGPAVRHYNPLPIPDASPWHAMDILGGGSL"
     gene            1392..2762
                     /locus_tag="VG274_04285"
     CDS             1392..2762
                     /locus_tag="VG274_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05901.1"
                     /translation="MAIVVQIGRAQAQATTQPEPIELRVRPMALAEPALKDRLLPPYS
                     EQTPGNAATLYLTAFSLVQSNSDSWDQKRSDEFDELLDASIDDLDAEKARRFLVPNVF
                     DLLEIAGRRSRCEWDTSMREQGYRALLPYLNQARTAANLLSIQIKLDIKQGDFDHAIR
                     MLRSGFAMANHLETEPILVQCLVAAGIRDVMLSDVRALIQRPNAPNLYWPLADLYGSR
                     DLWPRIMESEREALFYTIPRLRHPEDLTAEQARKVIEEINGYIEPSRGSDLQEALGRD
                     AATLLQIIRAYPLAREALIRRGTPAAQVDAIPANAATLAWDVDGFQRRADAIERWTCL
                     APWQAFAGLRREALRHREDDDLNPLNRLIPRVSLAYLTLARGDRERALMQMVEAVRGY
                     AAAHGGSAPASLDDLSPDTPAPPDPLLGKGFDYSVEHGIVTLHAAAVPGDGQISEAVF
                     HIQIER"
     gene            2791..>3695
                     /locus_tag="VG274_04290"
     CDS             2791..>3695
                     /locus_tag="VG274_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05902.1"
                     /translation="MERLTTILISSMALLLVPNACMATPGGNDTAAVAPFVGNHTWVV
                     ARADLAAIDLDAVPQWMVQLADLPALKQFGADPRAHLDAMTDRWRKWAAQFKKAGGRT
                     LWVVIGIETDEPLPTIVLVVPLSPGSDELALTQLLSAGDHMNVSHEKGALIARSPRTR
                     AFPTRPRRRLARPELAAALEATGEAPLRIAIIPPADGRRVLESMLPPLPDGQPATVVM
                     RGILHASIAIEPPPKAAGHLVLQSEDAASAQALQKFMTSLEGLGSPDTRRWDAVPNFS
                     QRALAAFVADLGRGSRVRDNQVVVDV"
BASE COUNT          689 a         1176 c         1168 g          662 t
ORIGIN      
        1 cttggatctg gggcaatggc ttgccaaagc agcgcaatcg ggaaccgcgg agcacgtggc
       61 ggaacgacat gtctttgggc agatgttggc catagggccg cgacgtcctc cggcacgatc
      121 ccaacgacgt gacggccctg accagcctcg tcgatgaact ggccaacagc cgaaggctcg
      181 tctcttgctc gcgcctcccg ctatgaatgc gaaacggacc tccggtccga ccggggcgac
      241 acgccccggc tctgaaaaaa gcgatcacga tacgaatcga aaagcgctat aagtaatcgc
      301 ggcaagcgag gccttgcgcc tggccacaga atatggactc ttcgggcgtc tggcgcccgc
      361 aaggattttt tcctggaaaa catatgcccg gctgcgtcta agcgatggtc ggatgatgga
      421 tgaggcggaa ctggaacggt cgttcgaggc acacgcggcg gcgttggtgc tgtacgcgcg
      481 gcaatggctc gatcggtccg gggccgagga cgcggtgcag gaggcgttcg tccggctgct
      541 gctgcagccg accccgccgc gtcaggtgaa agcgtggctt taccgcgcgg tacgaaacga
      601 ggcgatcacg caatggcgtt cggcgcgcag ccgtggacga cgcgagcgcc gagccgcttt
      661 gcctgaaacg ttgttcgatt atgacacgac ggcagccggg gacgtggaag cgctgacgac
      721 ggccatcgcg aggttgccgc ctacccagcg agaaatcctc gtcctgcggg tgtggggcga
      781 gctaacgctc gcggagatct ccgaactgat cgaggcgcca gtctcgaccg tgtttcagaa
      841 ttaccggacg gcattaggaa cgatgagaaa gtggttggaa gaaccatgcc agaagaagac
      901 gcattgaaca ccgaagagcg cgaaactctc gcggctgtcg cccgattcaa gcccgccacg
      961 attcggctca acccctttcg gatccgatcg gaagcgcagc ggcgccgggc aaagcgccag
     1021 gtatggctat ggcgcggcat cgcggcggca ctcgcggcgg gcctgattgt ctcgtggatt
     1081 tggcggccgg gcagcgctat ccgcgagcat gtggtctatg tacgcctacc ggccccggca
     1141 atcgctcccg aaacggcatt gccccgatcc gccacgaccg agatcgagcc acgaatgacg
     1201 ccgcccctcg gcgatgatta tctcgcggtc cgcgaccgcg tgctcgcctt tggtattgac
     1261 gccttgcggc cgcatgggcc cgcagtgcgg cactacaacc cgctgccgat cccggacgcg
     1321 tccccctggc acgccatgga tatcctgggc ggaggttcac tatgagatcc cgaatttgcg
     1381 ttctcacgat cgtggcaatc gtcgtccaga tcggccgtgc gcaagcgcag gcgaccacgc
     1441 aacccgagcc gatcgagctg cgcgtccgtc ccatggcttt ggccgagcct gcgctcaagg
     1501 accggctgct accgccgtac tctgagcaaa cgccgggcaa cgcggcaacg ctctatctca
     1561 ccgccttttc attggtgcag tcgaattctg attcatggga ccaaaagcgg tccgacgagt
     1621 tcgatgagct gttggacgca tccattgatg atctggacgc agaaaaagcc aggcgcttcc
     1681 tggtgccgaa cgtatttgat ctgctcgaaa ttgccggccg tcgatcgcgc tgcgagtggg
     1741 acacgtctat gcgggagcaa ggataccgcg cgctgctgcc ttacctgaac caggcccgca
     1801 cggcagccaa cttgctctcg atccagatca agctcgacat caagcagggc gatttcgatc
     1861 atgcgattcg catgctgcga tccggttttg cgatggccaa ccaccttgaa accgagccaa
     1921 tcctggttca gtgtctggtc gccgcgggca ttcgcgacgt tatgttgagc gacgttcgag
     1981 cgctgatcca gcggcccaac gcccccaacc tgtactggcc cctggccgac ctgtacggct
     2041 cgcgcgattt gtggccgcgc atcatggaat ccgagcggga ggcattgttt tacaccattc
     2101 cacgacttcg ccacccagag gatctgaccg ccgaacaggc ccgcaaagta atcgaagaga
     2161 tcaatgggta catcgagcca tcgagaggat cagacctgca ggaggcgctc ggccgagacg
     2221 ccgcgactct tctgcaaatc attcgtgctt acccgctagc cagggaagct ttgatccggc
     2281 gtgggacgcc ggccgcgcag gttgacgcca tcccggccaa cgctgccacg ctggcatggg
     2341 atgtggacgg ttttcaacgg cgcgctgatg caatcgagcg atggacctgc ctcgcgccgt
     2401 ggcaggcttt tgccgggctc aggcgagagg cccttcgcca ccgcgaagac gacgacctca
     2461 accctctcaa ccgactgatt ccccgtgtgt ctctcgctta tttgacattg gcgcggggcg
     2521 atcgcgagcg cgcgctaatg cagatggtcg aagccgttcg cggttatgcc gccgcccatg
     2581 gcgggtccgc cccggcgtca ttggatgacc tttcgccgga cacgcccgcg ccgcccgatc
     2641 cacttttggg aaagggcttc gactacagcg tcgagcacgg gatcgtcacg cttcacgctg
     2701 ccgcggttcc cggtgacggc cagatatctg aggctgtctt ccatatccag atcgaacggt
     2761 agtcacgcgc cgggcgcaag aggaagcatt atggaaaggc tgacaacaat tctgatctca
     2821 tcgatggcgc tgctgctggt cccaaacgcc tgcatggcca cgccgggtgg taatgacacg
     2881 gccgctgtag cgccgttcgt cgggaatcac acatgggtgg tcgcccgggc cgacctggcc
     2941 gcgatcgatc tcgacgccgt gccccaatgg atggtgcaac tggcggatct accggcgttg
     3001 aagcagttcg gcgctgatcc ccgggcacat ctcgacgcga tgacggaccg ctggcggaaa
     3061 tgggccgcgc agttcaagaa ggcgggcggc aggaccctct gggtcgtcat tggcatcgag
     3121 acggacgaac cgctgccaac gatcgtcctg gtcgtgcctc tgtcgcctgg atcagacgag
     3181 cttgcgctga cacagttatt gagcgcgggc gaccacatga atgtcagcca cgagaaaggc
     3241 gccctgatcg cccgttctcc gcgcacccgg gcgttcccca cccggccacg ccgccggctg
     3301 gctagaccgg agttggccgc ggctctcgag gccacggggg aagcgccgct gagaattgcg
     3361 atcattcccc ccgctgacgg ccggcgcgtc ttggagtcga tgctcccgcc gttgcccgat
     3421 ggccagccgg ccacagtggt gatgcgcggt attttgcatg cgtccattgc gatcgagccg
     3481 cccccgaagg cagcgggaca cctggtcctc caatccgagg acgccgcttc ggcgcaagcc
     3541 ctgcaaaaat tcatgacctc gcttgagggc ttgggatccc cggacacccg caggtgggac
     3601 gcggttccga acttttcgca gagggcactc gccgccttcg tcgctgacct gggccgcggc
     3661 agccgcgtgc gggacaacca ggtggtcgtt gacgt
//
LOCUS       DASZMC010000218         4990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4484090,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000218 DASZMC010000000
VERSION     DASZMC010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4484090"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="VG274_04295"
     CDS             complement(<1..1486)
                     /locus_tag="VG274_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWE05903.1"
                     /translation="MDTGTLAFKSHPDRAVDHRSPSTTKIALFRSLFRGREDVYARRF
                     ESRKSGKSGYQPDCANEWIKGVCEKPRIKCTDCLHQRFLPVTDAVIRSHLQGRDNAGT
                     PFVMGIYPMLQDETCFFLAVDFDKGSWQADASAFLETCRRLKIPATLERSRSGNGAHV
                     WFFFEQAISASLARSLGSYLLTDTMERRPDLGLRSYDRFFPNQDTLPRGGFGNLIALP
                     LQAGPRSSGNSVFLDEKLSPLSDQWSFLSTIQKIDQPTIESLLHTPEASDRIVGVQVV
                     LDTEDEDSPWVAPPSRRSKERSLEGALPKVLELTLANEVYISKESLPPALRNRLVRLA
                     AFQNPEFYRAQAMRLPVYDKPRIIGCAEDHPAYIALPRGCLEDVRKLCSDLKISVNVR
                     DERHAGLPLEAEFRGELRSDQHVAARAMLAHDTGVLAATTAFGKTVVGAWLIAKRRVN
                     TLVLVHRRQLLDQWIERLQSFLELPEKLIGRIGGGRRQLGGKLDV"
     gene            1733..2719
                     /gene="dusA"
                     /locus_tag="VG274_04300"
     CDS             1733..2719
                     /gene="dusA"
                     /locus_tag="VG274_04300"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HWE05904.1"
                     /translation="MNSLTHTFSVAPMMEWTDRHCRYLHRLLTRHARLYTEMITAEAV
                     VRGDRARLIGFDPFEHPVALQLGGAEPARLAEASRIAEDFGYDEINLNIGCPSDRVQS
                     GRFGACLMREPALVAKCAAAMREAVSIPVTVKCRLGVDEQEPEESLRALIAASVSEGV
                     STFIVHARKAWLKGLSPKENRDVPPLDHELVHRVKRENPNLTIILNGGIATLDDAARH
                     LEFVDGVMMGRAAYQSPAILAGADARFFVGPARDLDEAVEDYILYVERRLGEGVPLNA
                     MTRHMLGLFNGRPGARLFRRHLSEHATRAGAHVAVLRAALAHVSPQPLSVAA"
     gene            2723..3559
                     /locus_tag="VG274_04305"
     CDS             2723..3559
                     /locus_tag="VG274_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWE05905.1"
                     /translation="MDILHTTSSGDLGVLALGLVIAGVVGGLAAGMLGVGGGIVVVPV
                     LYHVMATLGVDQSVRMHVAIGTSLAAIIPASLVSVRAHHNTGGVDATLVHRWAVPLLA
                     GTVIGSVVFASAGERTLAIIFALLALPVALFLALRGRDRRVAAHLSERIHGFVLPVLI
                     GGASAATGMGGATLASTSLISFGARAEATVGNASALGAIVALPAALGAVVAGLHAPAL
                     PPWSLGYVSLPGLVLIAPVLLVAEPAGAALAHMIDMKRLHLVFAALVAIITARMLWDA
                     LS"
     gene            complement(3578..>4990)
                     /locus_tag="VG274_04310"
     CDS             complement(3578..>4990)
                     /locus_tag="VG274_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWE05906.1"
                     /translation="YDARWLFGREINLLGVEALGLGLGTYFYERGVVPRVAVGHDYRS
                     YSLSIKQALTIGLMRAGCDVVDIGMVTTPVAYFAQFALDAPCVAMVTASHNENGWTGV
                     KMGAERPLTFGPDEMLRLKDIVLGGNGLPRAGGGFSRASGIRARYLDAVAEGHKLSRP
                     MRVAVACGNGTAGAYAPEALRRIGAEVIEVDCALDYTFPNYNPNTEDMKMLHAMADAV
                     RAHRAEICLGFDGDGDRCGVVDDRGEEIFADKVGVLLARDLSARHEGAKFVVDVKSTS
                     LFETDPVLKARGVITDYWKTGHSYIKRRTAELHAVAGFEKSGHYFFNPPVGHGYDDGI
                     VAAIAVLEMLDRAGGKKMSELVAGLPKTWGTPTMAPHCPDEKKYAVVAALTAEYEGLK
                     ARGEKLLGQNIARIVTVNGVRVSLEDGTWGLVRASSNKPSLVVVAESPTSEANMRAMF
                     HDLDTRLSRHPEVGAYDQKI"
BASE COUNT          896 a         1559 c         1550 g          985 t
ORIGIN      
        1 cgacatccag ctttccgcca agctgccttc gtcctccccc aattcgaccg attaacttct
       61 cgggcaattc cagaaaggac tgcaatcgct cgatccattg atcaagcaat tgccgtcgat
      121 gcaccagcac gagagtgttg acgcgtcgtt ttgcgatcaa ccatgcgcca acgaccgttt
      181 ttccgaatgc agttgtggcc gcgagtacgc cggtgtcatg ggccaacatc gcccgcgcgg
      241 cgacatgctg atcggaccgc aattctccgc gaaactcggc ctcaagcggc agtccagcgt
      301 gtctttcatc tctcacgttt acggagatct tcaagtcgga acaaagcttc cgtacgtcct
      361 cgagacagcc gcgcggtagt gcgatgtacg cgggatgatc ttccgcacaa ccaatgatcc
      421 tcggcttgtc ataaaccggc agccgcatcg cctgggcgcg atagaattcc ggattctgaa
      481 aagccgccaa gcgaacaagc cgattgcgaa gcgctggtgg caacgattct ttcgaaatgt
      541 atacctcgtt ggcgagagtc agttctaaaa ccttcggcag ggcgccctca agtgaacgct
      601 ccttcgagcg acgagatggc ggtgctaccc acgggctatc ttcgtcctcc gtgtccagaa
      661 cgacttgtac gccaacgatc cggtccgacg cttccggcgt atggagaagg ctttcaatcg
      721 ttggctgatc aatcttctga attgtcgaga ggaaggacca ttggtccgaa aggggcgaga
      781 gcttctcatc aaggaagacg gagtttcccg agcttcgcgg tcccgcctgc agcggtaaag
      841 cgattagatt tccgaagccg ccccggggga gcgtatcctg attggggaaa aaacggtcat
      901 aggacctgag gccgaggtct ggacgacgtt ccatcgtgtc ggtcagaaga tatgaaccga
      961 gactccgggc caaggacgcc gatattgcct gctcgaagaa gaaccagaca tgagcaccgt
     1021 tgccggatcg agatcgctcc aacgtcgccg gaatcttcaa tctgcggcaa gtttcgagaa
     1081 atgcgcttgc atccgcttgc cagctacctt tgtcgaagtc gaccgcgaga aagaagcagg
     1141 tttcgtcttg gagcatcgga taaatgccca tcacgaacgg tgttcccgca ttatcgcggc
     1201 cctgcaggtg cgatcgaatg accgcatcgg tcactgggag aaatcgttgg tgcaggcaat
     1261 cggtgcattt gattctgggt ttttcgcaga cgcccttgat ccattcgttt gcacagtccg
     1321 gctgatagcc cgacttgccc gatttccggc tttcgaagcg acgtgcatag acatcctctc
     1381 gcccgcgaaa tagcgagcgg aaaagcgcga tcttggtggt cgagggagat cgatgatcga
     1441 ctgcgcgatc aggatggctc ttgaaagcga gggttccggt gtccactact tatgtaccct
     1501 gcgtcggaca gactggcgtc gttcccatct tggagtagac agacggtccg gctgcgccaa
     1561 ctgcttgacc tttctcgtgc acgaatacga gcacaaatcc atcacactca gaatgccatc
     1621 tcggctccaa ggtcggtcgc gtggcgtaaa atcggatagg caaagcaagc acttgctagc
     1681 ggcgcgtcga ttttcgttga agattcggcc aaaatgaaat ttattcaata caatgaatag
     1741 cttaactcac acattttctg tcgcgccgat gatggagtgg acggaccggc attgccgcta
     1801 tctccaccgc cttttgacgc gccacgcgcg gctctacacg gaaatgatca ccgccgaggc
     1861 ggtcgtccgc ggggatcgcg cgcggctgat cggcttcgac ccgttcgagc atccggtagc
     1921 gctgcagctt ggcggcgccg aacccgcgcg gctggccgaa gcgtcgcgga tcgccgaaga
     1981 ttttggctat gacgagatca acctgaatat cggctgcccc tcggatcgcg tgcagtcggg
     2041 gcgcttcggc gcttgtctca tgcgcgaacc cgcgcttgtt gccaaatgtg ccgcagccat
     2101 gcgcgaagcg gtaagcattc cggtgactgt caaatgccgg ctcggcgtgg atgagcagga
     2161 gcccgaggaa agcctgcgcg cgctgatcgc agcgtcagtc tccgaaggcg tgtcgacctt
     2221 catcgttcat gcgcgcaagg cgtggctcaa gggattgtcg ccgaaggaga accgcgatgt
     2281 cccgccgctc gaccacgaac tcgtccatcg ggtgaaacgc gagaatccga atctgacgat
     2341 catcctcaat ggcggcatcg cgacgctcga cgacgcagcg cggcatctgg aattcgtgga
     2401 cggagtcatg atgggccgcg cagcctatca atcgccggcg atactcgcgg gcgccgatgc
     2461 gcgattcttc gtgggtccgg cgcgcgatct ggatgaagcg gtggaggact atattcttta
     2521 cgtcgagcgg cggctgggtg agggcgtgcc gctcaacgca atgacccgcc acatgctggg
     2581 gcttttcaac ggcaggcccg gggcgcgcct gttccggcgc catctcagcg aacacgcgac
     2641 gcgtgcgggc gcccacgttg ccgtgctgcg cgcggctctg gcgcatgtct cgcctcagcc
     2701 attgagcgtg gccgcgtagc ccgtggacat cctgcacacg acgtcttcgg gcgatctcgg
     2761 cgtgcttgcg ctcggactgg tcattgcggg cgtggtgggc ggactcgccg ccggcatgct
     2821 gggcgtcggc ggcggcatcg tggtggttcc ggtactctac cacgtcatgg cgacgctcgg
     2881 tgtcgaccaa agcgtgcgca tgcacgtcgc gatcgggacg tcgctggccg cgatcattcc
     2941 cgcttctctc gtatccgtac gcgcccacca caacactggt ggagtcgatg caacgctcgt
     3001 gcaccgctgg gccgtgccgc tgcttgcggg gacggtgatc ggaagcgtgg tgttcgcgag
     3061 cgcaggcgaa cggacgcttg caatcatctt cgcgcttctc gcgctgccag tcgcgctgtt
     3121 tcttgcactg cgtggccgcg atcgccgcgt cgcggcgcat ctttccgagc gcatccacgg
     3181 attcgtgctg cccgttttga tcggcggcgc atcggctgcg accggaatgg gcggcgcgac
     3241 gctcgcttca acatctctca tctcgttcgg cgcgcgggcc gaagcgaccg tgggcaatgc
     3301 gtcggcattg ggcgccatcg tcgcgctccc cgctgcgctc ggcgcagttg ttgcggggct
     3361 gcacgcgcct gccctgccgc cctggtcgct cggctatgtc agcctgcccg ggctggtgct
     3421 gatcgcgccg gtactcctcg tggcggaacc ggccggcgcc gctctcgcgc acatgatcga
     3481 catgaaacgc ctgcaccttg tctttgcggc cctggttgcg atcatcaccg cccgcatgct
     3541 gtgggacgcg ctcagctgaa gaattgcggc ccgccgctca tatcttctgg tcgtacgcgc
     3601 cgacctcggg gtgccgggaa aggcgggtgt cgagatcgtg gaacatggcg cgcatgttgg
     3661 cttccgatgt cgggctttcc gcgacgacga ccagagaggg cttgttggag gacgcccgga
     3721 cgagtcccca ggtgccgtct tcgagcgaca cgcgaacgcc attgaccgtc acgatgcggg
     3781 caatgttctg gccgagcagt ttttcgccgc gcgccttcag cccttcatat tccgcggtca
     3841 gcgcagcgac caccgcatac tttttctcgt ccggacaatg gggcgccatg gtgggcgtgc
     3901 cccaggtctt gggcaggccg gcgaccagtt ccgacatctt cttgccgccg gcgcggtcga
     3961 gcatttcgag caccgcgatc gccgccacga tcccgtcgtc gtagccgtgg ccgaccggcg
     4021 gattgaagaa ataatggccg ctcttctcga agcctgccac ggcgtgcagt tccgcggtgc
     4081 gccgcttgat gtaggaatga cccgtcttcc aatagtcggt gatgacgccg cgcgccttca
     4141 gcacgggatc ggtttcgaac aggcttgtgg acttcacgtc gaccacaaac tttgcgccct
     4201 cgtgccgcgc ggagagatcg cgcgcgagca gaacgccgac cttgtcggcg aagatttcct
     4261 cgcccctgtc gtcgacgaca ccgcagcggt cgccgtcgcc gtcgaagccg aggcatatct
     4321 ctgcacggtg cgcgcgcacc gcgtcggcca tcgcatgcag catcttcatg tcttcggtgt
     4381 tgggattgta gttggggaag gtgtagtcaa gagcgcaatc gacttcgatc acctccgcgc
     4441 cgatgcggcg cagcgcctcc ggcgcgtagg cgccggccgt gccgttgccg caggcgaccg
     4501 cgacgcgcat cggccgcgac agcttgtgtc cctccgccac cgcatcgaga tagcgcgcgc
     4561 ggatgccgct tgcgcgggag aagccgccgc cggcgcgcgg caggccattg ccgcccagca
     4621 cgatatcctt cagacgcagc atctcgtcgg gtccaaaggt gagcggacgc tcggctccca
     4681 tcttgacgcc ggtccagccg ttctcgttgt ggctcgccgt caccatggca acgcagggcg
     4741 cgtcgagcgc gaattgcgcg aaataggcga cgggtgtcgt caccatgccg atatcgacga
     4801 cgtcgcagcc ggcgcgcatc agtccgatgg tgagcgcctg cttgatcgaa agagaatacg
     4861 accggtagtc gtgcccgacc gcgactctgg gaaccacgcc gcgctcgtaa aaataggtgc
     4921 cgagccccag acccagcgcc tcgaccccca gaaggttgat ctccctgccg aagagccagc
     4981 gggcgtcgta
//
LOCUS       DASZMC010000219         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3941123,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000219 DASZMC010000000
VERSION     DASZMC010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3941123"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..>2071)
                     /locus_tag="VG274_04315"
     CDS             complement(126..>2071)
                     /locus_tag="VG274_04315"
                     /inference="COORDINATES: protein motif:HMM:NF009379.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWE05907.1"
                     /translation="ITGAIQRKGSVPQRNTVGDSSPEARARQMSVEVNCATTKYLDES
                     FTFLDCPGSIEFLQDTLNVLPGVDAAVVVCEPDPAKVQMLKPFLKRLSDLSIPHFLFI
                     NKIDKAQGALRDLLAGLQEASDKPLLLRQIPIWEDGIVTGFVDLALERAYVYREHDES
                     KIVDLGGDVKEEEKQARFSMLEKLADYDEHLMEELLSDVEPPRDEVFGDLSKELAEGL
                     VVPALIGSAEKDNGIRRLLKALRHEVPELTATAARLGVRTDGEAVAQILKTYNGAHGK
                     LSLVRVFAGPLKDGAVLHASDGRDARVAGIFALKGDAQTKLTEAKAGETVAVARLEGM
                     VTGDTLSGAKSPAANGAIEALEPVYALAIETTDRKDEVKLTAAIHKIQEEDPSLHFRQ
                     NAELQEMSLAGQGEIHLKVAAEKLASKFGLKITTHQPKIPYKESIRKPGRQHGRHKRQ
                     SGGHGQFGDVHIEIRPLPRGTGFVFEDLIKGGVVPRQFIPSVEKGVVDFLKQGPLGFP
                     VVDVGVALVDGSYHTVDSSDAAFQTAARIAMTEGMPNCAPVLLEPIMRVKIHIPNDAT
                     SKVNQVVSGRRGQLMGYDARPGWKGWDTVDAQMPLAELHGLIIDLRSLTQGVGTFEME
                     FDHLAELTGKLADHVVASHKAAA"
BASE COUNT          363 a          673 c          612 g          423 t
ORIGIN      
        1 gcagggcgaa aatttcgctt cgtcgtttcc cgctttgagt taagcgggag acacccaagc
       61 aacgtaagcc ggggcgttca ttcgccacgg ctcctttttc atctccgtca cagaaccggg
      121 cgctctcagg ccgccgcctt gtgcgacgcc acaacatggt cggcgagctt gcccgtaagt
      181 tccgccaaat ggtcgaattc catctcgaac gtgcccacgc cctgcgtcag cgaccgcaga
      241 tcgatgatga gaccgtgcag ttcggccaga ggcatctgtg cgtccaccgt atcccagccc
      301 ttccagcccg gccgcgcgtc gtaacccatc agctgtccgc gccgcccgct caccacctga
      361 ttgaccttcg acgtggcgtc gttgggaata tgaatcttca cccgcatgat tggctcgagc
      421 agaacaggtg cgcagttcgg catgccttcc gtcatggcga tgcgcgccgc cgtctggaag
      481 gcggcgtcgg acgaatcgac ggtgtgatag gaaccgtcga ccagcgcgac gcctacatcg
      541 accacaggaa aaccaagcgg accttgcttc agaaaatcga cgaccccctt ctcgaccgaa
      601 gggatgaact gcctggggac cacgccgccc ttgatcagat cctcgaatac gaatcccgtg
      661 ccgcgcggca gcggcctgat ctcgatatgc acgtcgccga actggccgtg cccgcccgat
      721 tggcgcttat ggcggccatg ctgccgtccg ggtttccgga tcgattcctt gtagggaatc
      781 ttcggctgat gcgtcgtgat cttgagcccg aatttgctcg cgagcttctc ggcggcgact
      841 ttcagatgaa tctcgccctg gcccgccagc gacatttcct gcaactccgc attctgccgg
      901 aaatgcagcg acgggtcttc ctcctgtatc ttgtggatcg cggcggtaag cttgacctcg
      961 tccttgcgat ccgtcgtttc gatggcgagc gcgtaaaccg gttcgagcgc ctcgatggcg
     1021 ccattcgcag ccggcgattt tgcgccagag agagtgtcgc cggtcaccat gccttcgaga
     1081 cgcgcgacgg cgacggtttc tccggccttt gcctcggtga gctttgtctg cgcgtcgccc
     1141 ttcaaggcga agatgcccgc gacccgcgcg tcgcgaccgt cgcttgcgtg caacacagcg
     1201 ccatccttga gcgggcccgc gaaaacgcgc acgaggctca gctttccgtg ggcgccatta
     1261 taggtcttca atatctgcgc caccgcttcg ccatctgtcc tgacgccgag gcgcgctgcg
     1321 gtggccgtca gctccggcac ttcgtgcctc agggctttga ggagccggcg gatgccgttg
     1381 tccttctctg cggagccgat cagcgcagga accacgagcc cttcggcaag ctccttcgaa
     1441 aggtcgccga agacttcatc gcgaggcggc tcgacgtcgg aaagcagctc ttccatcaga
     1501 tgctcgtcgt agtcggcgag cttttcgagc atcgagaacc gcgcctgctt ctcctcttcc
     1561 ttcacgtcgc cgcccagatc gacgatcttc gattcgtcgt gctcgcgata cacgtaggcg
     1621 cgttcgagcg cgagatcgac gaaacccgtc acgatcccgt cttcccagat cggtatctgc
     1681 cgcagcagca gtggcttgtc gctcgcctcc tgcagcccgg cgagcagatc gcgcaacgca
     1741 ccctgcgcct tgtcgatttt gttgatgaag aggaaatggg gaatggaaag atcggagagg
     1801 cgcttgagga acggtttcag catctgcacc tttgccgggt cgggctcgca gacgacgaca
     1861 gccgcatcga cacccggcaa gacattgagc gtatcctgaa ggaattcgat cgagcctggg
     1921 caatcgagaa aggtgaaact ctcgtcgaga tatttcgtcg tcgcgcagtt gacctcgacg
     1981 ctcatctgcc gcgcccgggc ttcgggcgac gagtccccaa cggtgttgcg ctgcggtacg
     2041 gagcccttgc gctggatcgc gccggtgatg g
//
LOCUS       DASZMC010000220        10123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1211584,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000220 DASZMC010000000
VERSION     DASZMC010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211584"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..449)
                     /locus_tag="VG274_04320"
     CDS             complement(45..449)
                     /locus_tag="VG274_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWE05908.1"
                     /translation="MLHISAETLPAALYRDPILYETERRRIFARSWQLVGHDAQFAAR
                     GSWLAVTVAGFPLLLVRGDDGRIRAFHNVCRHRAGPLAPDGQGICDGTLVCRYHGWRY
                     ALDGRLAAARDFGPAEGFDVREYGLFELACEG"
     gene            540..1397
                     /locus_tag="VG274_04325"
     CDS             540..1397
                     /locus_tag="VG274_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002049937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWE05909.1"
                     /translation="MTPRRFEGTDRYIATDDLRIAVNASVTLERPLLIKGEPGTGKTQ
                     LAYEVAEALAAPLITWHVKSTTKAQQGLYEYDAVTRLRDSQLGDARVKDVRNYIKRGK
                     LWDAFDMGERPVLLIDEIDKADIEFPNDLLLELDRMEFFVYETGETIRARLRPVVMIT
                     SNNEKELPDAFLRRCFFHYIKFPDRETMEKIVDVHFPDIKQRLVSEALNVFYRIREVP
                     GLKKKPSTSELLDWLKLLLNEDIPPESLRERDPAKLIPPLHGALLKNEQDVHLFERLA
                     FLARREARG"
     gene            1541..2095
                     /locus_tag="VG274_04330"
     CDS             1541..2095
                     /locus_tag="VG274_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05910.1"
                     /translation="MTIILVCANANAQTVAGDTFADGKDGAKVHTASGFVCPAKIGLF
                     ERDAVGESNPETGEDFCAYGALDGVYGTIKLTPLHGAYNAEQSLAGEFAEQEGTVGKR
                     IAQKDVPVGSAPNAPPLVVFTRTYEAAKLEDLHYRVQFAGAAVNNWAVEATIEFAEPR
                     DTPLAQDFFHAVYAAAQTEIGAAH"
     gene            2111..3286
                     /locus_tag="VG274_04335"
     CDS             2111..3286
                     /locus_tag="VG274_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWE05911.1"
                     /translation="MFFQFFDELRSARLPVTLKEYLALLDALSSGVIDMKTDEFYYLS
                     RAALVKDERNLDKFDRVFSHVFKGVETLDATEVAQLPEEWLRALTEKFLTEEEKKQIE
                     ALGGWEKLMETLKKRLEEQKKRHEGGSKMIGTAGTSPFGAMGYNPEGVRIGQDRGRHG
                     SAVKVWDRREYRNLDDSVELGTRNIKIALRRLRKWARTGAEVELDLPGTIKETANRGY
                     LDLKLIPERHNTVKVLLFFDIGGSMDAHIRLCEELFSAAKSEFKHMEFFYFHNCLYEA
                     VWKDNRRRHSEKSATWDVLRKFPHDYKVIFVGDATMSPYEITYPGGSVEHWNEEAGAV
                     WLDRVAQIYGHTVWLNPVPVKHWEYTPSVGLIRQLLSDRMYPLTLAGLDHAMRELNR"
     gene            3330..3974
                     /locus_tag="VG274_04340"
     CDS             3330..3974
                     /locus_tag="VG274_04340"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWE05912.1"
                     /translation="MLTVHHLNNSRSQRILWLLEELDVPYEIAKYQRMSPIPLAPPEL
                     KQVHPLGKSPVITDGSHTIAESGAIIEYILERYGRGRMRPDAKSDDHFRYLEWMHYAE
                     GSAMVPLLLHLYAGFLGEGAAPLRPRIDSEIASHLSYIGDALDGREFLVGHSLTGADI
                     QIAFVLEAAQSSGFLKDRPAMEQYLARLQTRPAYKRAIDRGGPYDLAANRRPNS"
     gene            complement(3984..4442)
                     /locus_tag="VG274_04345"
     CDS             complement(3984..4442)
                     /locus_tag="VG274_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWE05913.1"
                     /translation="MQARMRNIAIVIPEIVPTTQALAACAEKGGLPARTVGLVHLRAS
                     QINGCSVCVDMHPKMMKKAGETDQRLFAVAAWRDASYFTDAERAALALTEAVTRLSDR
                     ADPVPDEIWNEAARHYDETGLAALLIQIAVINVWNRLNVATRQVAGQWGG"
     gene            4553..5428
                     /locus_tag="VG274_04350"
     CDS             4553..5428
                     /locus_tag="VG274_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWE05914.1"
                     /translation="MNEQILLADQSGANRPRLRAVAYRMLGSLGEADDAVQETWLRLA
                     RSDSDEIGNLGGWLTTVLARVCLDMLRSRKSRREEPLESSAVEPGRHVESTINPEQES
                     MLADSVGIALLVVLQTLDPAERVAFVLHDMFDLPFEEIAAILGRSPVAARQLASRARR
                     RVRKPAQIPGEELARQRKVVDAFLAASHDGDLNALLAVLDPDVVFRADDAAAKAGARS
                     EIRGAAEVARTFKGRAQGARPALVNGSIGLLVVRSGRLFVVLVPRIAEGKIVEIDAVA
                     DSGHLRRLELSVLSD"
     gene            complement(5464..5850)
                     /locus_tag="VG274_04355"
     CDS             complement(5464..5850)
                     /locus_tag="VG274_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HWE05915.1"
                     /translation="MTDWIWIDPDVLLAAHDEQLEEHGGASGIRDRGLFESALARPKN
                     TAAYGEPDAAELAAAYGFGLAKNHAFVDGNKRIAFVALELILLLNGFELHADDVQCVV
                     VMLSMASGAFSEAELTEWVRRNMKPA"
     gene            complement(5847..6092)
                     /locus_tag="VG274_04360"
     CDS             complement(5847..6092)
                     /locus_tag="VG274_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWE05916.1"
                     /translation="MLERTPMYYTVKVIQIGNSLGVTLPKEALAALKVDKGDALTLTP
                     APDGFRVASYDAERIVQLEAARKIMKKRRHALRELAK"
     gene            complement(6142..6699)
                     /locus_tag="VG274_04365"
     CDS             complement(6142..6699)
                     /locus_tag="VG274_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05917.1"
                     /translation="MTGAALRRLGSASAVLLLHFALLTAFIFAMNSRLPGRNPAREIV
                     VSFPAVIRKPPAPVVLRPDLVAPVMPLQGPTSPELSMPELTPAPPGPGSISGVGRALF
                     GCDPLKIDTLPPEERAACLPPARPREKSVRFGPPPDSNSPWAKVIAERFREAKPINHP
                     CSLGSYNDIHGLPCLFGSDDPPPHQ"
     gene            complement(6715..6921)
                     /locus_tag="VG274_04370"
     CDS             complement(6715..6921)
                     /locus_tag="VG274_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWE05918.1"
                     /translation="MIVVNLDVMLARRKMRSKELAELIGITEANLSLLKSGKVKGVRF
                     STLEKICVELACQPGDLLEYRADA"
     gene            complement(6921..7466)
                     /locus_tag="VG274_04375"
     CDS             complement(6921..7466)
                     /locus_tag="VG274_04375"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HWE05919.1"
                     /translation="MIVSKGLARASRIMAWFSAAAVFIVPLGTAFMFLAPQWARPLGL
                     DMDRHGLSHLTAAVPLLDRALALACAMIPATIATFGLVALMRLLVLFNRGQVFGSDAL
                     RAFGRITAALFWNVLAAFVMEAPITYFLTRDHPPGQHGELQLTIGSDDVQVLFLAGVA
                     WVVSRVMAEARRVADENAGFV"
     gene            7665..7835
                     /locus_tag="VG274_04380"
     CDS             7665..7835
                     /locus_tag="VG274_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05920.1"
                     /translation="MSRLNFNVNKYDNSPRRRSYNPRGKVALRTPLHLSGVYLFAVAT
                     AMLVSFAIVWYA"
     gene            complement(7852..8160)
                     /locus_tag="VG274_04385"
     CDS             complement(7852..8160)
                     /locus_tag="VG274_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWE05921.1"
                     /translation="MKIVATDRNGREHVLDGRDGWSVMEILRDAGLPVAAECGGACAC
                     ATCHVYIDGEWYAKLAPPSAAEVDMLDMALAVEPTSRLSCQIVCTPALDGIKLTIAPE
                     "
     gene            complement(8157..9179)
                     /locus_tag="VG274_04390"
     CDS             complement(8157..9179)
                     /locus_tag="VG274_04390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWE05922.1"
                     /translation="MAIETDVAILGAGPIGLFAVFELGLLDLKAELVDILDRPGGQCT
                     ELYPEKPIYDIPGLPVVSGQELTDRLMDQIKPFHAGFHLSQMASGLERLADGRWKLST
                     DAGAEIVAPVVVIAAGAGSFVPKRPPIAGIDAYEGKSVHYAVRKMESLRGRNIVIAGG
                     GDSALDWTINLAPLAGSLTLVHHRDGFRAAQHSVNRMRELAAEKRIVFHVASVKALHG
                     AGGQLEAVTLSGADKKEWTHECDTFLPFFGLTIKLGPIAEFGLNLHENLVPVDTAKFE
                     SSTPGIFAIGDINAYAGKLKLILCGFHEAALMAQAAFHICRPNEKLRFQYTTSSSSLQ
                     KKLGVA"
     gene            complement(9272..9622)
                     /locus_tag="VG274_04395"
     CDS             complement(9272..9622)
                     /locus_tag="VG274_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWE05923.1"
                     /translation="MSSEPQLIDLAHLARYTGGEARLDAEVLELFSAQTAAMVERLET
                     ALERSDVTAWRELTHSLKGAANGIGAFALGEAAADAESVDPATDPRRAVEAFHAMKRC
                     ADVVSSFIDAYLGR"
     gene            complement(9693..>10123)
                     /locus_tag="VG274_04400"
     CDS             complement(9693..>10123)
                     /locus_tag="VG274_04400"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HWE05924.1"
                     /translation="PSPTRVQVGGAAATQSDAISGVLRITRHPFLWGVVIWSGFHVAA
                     NGDEASVIFFGTFFLLSLAGTFSIDAKRKRKLGAAWDAFASRTSNIPFAAVIAGRGQL
                     HLGESFGWRFWVAALLFLATLFAHARIFGVSPFPGGWVPF"
BASE COUNT         2034 a         3180 c         3090 g         1819 t
ORIGIN      
        1 agcgaaggcg ccgtcgcatc gatgttgacg aagacgaatc cgcgtcagcc ttcgcaggcg
       61 agctcgaaca ggccatattc acggacgtcg aaaccctccg ccgggccaaa atcgcgtgcg
      121 gccgcaagcc ttccatccag cgcgtagcgc cagccgtgat agcggcagac aagcgtgcca
      181 tcgcaaatgc cctgtccatc cggcgccagg gggccggcgc ggtgacggca gacattgtgg
      241 aaagcgcgga ttcgtccatc gtcgccgcgg acgagaagca gcggaaagcc ggcgaccgtt
      301 acggcgagcc acgagccgcg tgcggcgaac tgcgcgtcat gcccgacgag ctgccacgac
      361 cgcgcaaaga ttcgccgacg ctccgtctcg taaagtatgg gatcgcggta gagagcggcg
      421 ggaagcgttt cggcggaaat gtgaagcatg ggtgaaatcc tatcaggtgg attgccggct
      481 tgcgaaccgg gaggtgcgcc cctaggctcg accgaacgct atccctgaac gagaaaacga
      541 tgacgccgcg ccgtttcgaa ggaaccgatc gatatatcgc gaccgacgat ctgcgcattg
      601 cggtcaacgc gtccgtcacg ctcgaacggc cgctcctgat caagggcgag cccggcaccg
      661 gcaagacgca gcttgcatat gaagtggccg aggcgctcgc ggcgcccctc attacctggc
      721 atgtgaagtc tacgaccaag gcgcagcagg gtctttacga atacgatgcc gtgacgcggt
      781 tgcgcgattc ccagctcggc gatgcgcgcg tcaaggatgt ccgcaactac atcaagcggg
      841 gcaaattgtg ggacgcattc gacatgggcg aacgcccggt cttgctcatc gacgagatcg
      901 acaaggccga tatcgaattt ccgaacgacc ttctcctcga actcgatcgc atggaattct
      961 tcgtgtacga gacgggcgaa acgatcaggg cgcgtctgcg gccggtcgtg atgatcacgt
     1021 ccaacaacga gaaggagctg cccgacgcct tcctgcgccg ctgcttcttc cactacatca
     1081 aatttcccga ccgcgagacg atggaaaaaa ttgtcgacgt gcactttccc gacatcaagc
     1141 agagactcgt gtcggaagcg ctgaacgtct tctaccggat tcgcgaagtt cccggactga
     1201 aaaagaagcc gtcgacatcg gagcttctcg actggctgaa gcttctcttg aacgaggaca
     1261 ttccgcccga atcgctccgc gagcgcgacc ccgccaagct cattccccct ctccacggcg
     1321 cgctgctcaa aaacgaacag gacgtgcacc tcttcgagcg cctggcgttt ctggcgcgga
     1381 gggaggcgag gggatgatgc gcggtcgctc cctccgcaat tcttgcgtgt catccccgcg
     1441 caagcgggga cccagtaccg tgtcgctgaa gggttttctg tgcgcgcgga cgcgcgctag
     1501 ctgggttctc ccccggttaa acggggggat catcgcggga atgacaatca tattggtatg
     1561 cgcaaatgcc aatgcccaaa ccgtcgctgg cgacaccttt gcggacggca aggatggcgc
     1621 gaaggtgcat acggcgtcgg ggttcgtgtg tcctgcaaag atcgggctct tcgaacgcga
     1681 cgcggtcggc gaatccaatc cggaaaccgg cgaggatttc tgcgcctatg gggcgctcga
     1741 cggcgtctat gggacaatca agctcactcc gctgcacggc gcgtataacg cggagcaatc
     1801 cctggccggc gaattcgccg aacaggaagg aacggtcggc aaacgcatcg cgcagaagga
     1861 cgtgcccgtg ggatcggcgc ccaatgcgcc cccgctcgtc gtttttaccc gcacctacga
     1921 agccgcaaag ctggaagatc tgcactaccg cgtacaattc gccggcgccg cggtgaacaa
     1981 ctgggccgtc gaggccacaa tcgaattcgc cgaaccgcgc gacactccac tggcccagga
     2041 ttttttccac gccgtctacg ccgccgcgca gaccgagatc ggcgctgcgc attgagagaa
     2101 ccgaccggcg atgttcttcc agttcttcga tgagcttcgc tcggccaggc taccggtcac
     2161 gctgaaggaa tatctcgcac tgctggacgc gctttcgagc ggcgtcatcg acatgaagac
     2221 ggacgaattc tactacctct cgcgcgcggc gctggtgaag gacgaacgca acctcgacaa
     2281 gttcgatcgc gtgttctccc atgtgttcaa gggcgtggaa acgctcgacg caaccgaagt
     2341 cgcgcagctc cccgaggaat ggctgcgcgc gctgacggaa aaattcctca ccgaggagga
     2401 gaagaagcag atcgaggctc tgggcggctg ggaaaagctc atggagacgc tgaagaagcg
     2461 tctcgaggag cagaagaagc gtcatgaagg cggctcgaag atgatcggca cggccggcac
     2521 ctcgccgttc ggcgcgatgg gctacaatcc cgaaggcgtg cgcatcggcc aggacagggg
     2581 ccgccacgga agcgcggtga aagtctggga ccggcgcgaa tacaggaacc tcgacgattc
     2641 cgtcgaactc ggcacgcgca acatcaagat tgcgctgcga cgcctgcgca aatgggcgcg
     2701 gacgggcgcc gaagtggagc tggatcttcc ggggaccatc aaggaaacgg cgaacagggg
     2761 ttacctcgat ctcaagctga tacccgagcg ccacaacacg gtgaaggtgc tcctgttctt
     2821 cgacatcggc ggatcgatgg acgcgcacat caggctttgc gaggagctgt tcagcgcggc
     2881 gaagtccgaa ttcaagcaca tggagttctt ttacttccac aactgcctct acgaagcggt
     2941 gtggaaagac aatcgccgcc gccacagcga gaaatccgcg acctgggacg tgctccgcaa
     3001 attcccgcac gactacaagg tgatcttcgt cggcgatgcg acgatgagcc cctacgagat
     3061 cacctatccg ggcggctcgg tggagcactg gaacgaagaa gcgggcgcgg tgtggctcga
     3121 ccgcgtggcg cagatctacg gacacacggt gtggctcaat cccgtgcccg tgaagcattg
     3181 ggaatacacg ccctcggtgg ggctgatccg gcagcttctc tcggatcgca tgtatccgct
     3241 tactctcgcc ggcctcgacc acgcgatgcg ggaactgaac cgctgaccgg ttcgcgcgtt
     3301 ctggtcacct tgcaacacgg agatctgtca tgctgacggt gcaccatctg aacaattccc
     3361 gctcgcagcg gattctctgg ctgctcgaag agctggatgt cccctatgag atcgcgaaat
     3421 accagcgcat gtcgccgatt ccactcgcgc cgccggagtt gaagcaggtg catccgctag
     3481 gcaagtctcc ggtaatcacc gatggcagcc acacgatcgc cgagtccggc gcgatcatcg
     3541 aatatattct cgagagatac ggccgcggcc gcatgcggcc ggatgcaaag tccgacgatc
     3601 atttccgcta tctcgaatgg atgcattatg cggaaggatc ggcgatggtg ccattgctgc
     3661 tgcacctcta tgcgggcttt ctcggcgagg gcgccgcacc tttgcggccg cgaatcgaca
     3721 gcgagatcgc aagccatctt tcctacatcg gcgatgcgct tgacgggcgc gaattccttg
     3781 tcggacattc gctcaccggc gccgacattc aaatcgcttt cgtgctcgag gctgcgcagt
     3841 cgagcggctt cctgaaagat cggccagcga tggagcagta cctcgctcgc ctgcagaccc
     3901 gtcccgccta caaacgcgcc atcgacagag gcggacccta cgatctcgcc gcaaaccgcc
     3961 gcccgaacag ttagcagcct gtcttagccg ccccactggc ctgcgacttg ccgcgtggcg
     4021 acgttgaggc ggttccagac attgatcacc gcgatctgga tgaggagcgc cgcaagaccc
     4081 gtctcgtcgt aatgccgcgc ggcttcattc catatctcat cgggaaccgg gtccgcgcgg
     4141 tcgctgaggc gggtgacggc ctccgtcagc gccagcgccg ctcgttcggc gtcggtgaag
     4201 taggacgcgt cgcgccaggc cgcgacggcg aagagacgtt gatccgtttc cccggccttt
     4261 ttcatcatct tggggtgcat gtcgacgcag acgctgcagc cattgatctg gctggcgcga
     4321 agatggacga ggcccacggt ccgcgcgggc aaaccgcctt tttccgcgca ggctgccaag
     4381 gcctgggtgg tcggcacgat ttcgggaatg acgattgcga tgttgcgcat ccttgcctgc
     4441 atggttttct ccttcgctga gctgcccctg cccgtcggac aacaagctct gtcacacggg
     4501 ccgggtttca ttcgtcatct gcatgacgga ccggcttcca aagatgtgac cgatgaacga
     4561 acaaatttta ctcgcagatc aatccggggc caaccggcca cgcctgcgcg ctgtcgccta
     4621 tcgcatgctc ggctcgctcg gcgaggccga cgatgccgtt caggaaacgt ggctgcgcct
     4681 cgcccgctcc gactccgacg agatcgggaa tctgggcggc tggctgacga cggtgctggc
     4741 gcgggtctgt ctcgacatgc tgcgttcgcg caaatcgcgg cgcgaggaac cgcttgagtc
     4801 cagcgccgtc gaacccgggc gacacgtcga aagcacaatc aatcccgagc aggagtcgat
     4861 gctggccgac tccgtcggga tagcgctgct tgtcgtgctg caaacacttg atcccgccga
     4921 gcgcgtcgcg tttgtcctgc acgacatgtt cgacctgccg ttcgaggaga tcgccgccat
     4981 cctgggccgt tcgcctgtgg ccgcgaggca acttgccagc cgcgcccgcc gtcgcgttcg
     5041 caagccggcc cagatccccg gcgaggaact tgcgcgtcag agaaaggtcg tggatgcatt
     5101 cctggccgcg tcgcacgacg gcgatctgaa cgcgcttctc gccgtgctcg atccggacgt
     5161 cgtattccgt gccgatgatg ccgcggcgaa ggcgggcgcc aggagtgaaa tccgtggtgc
     5221 ggccgaagtg gcaagaacat tcaaaggccg tgcgcagggc gcacgcccgg cgctggtgaa
     5281 cggatcgatt ggtcttctcg tggtgcggag cggacgtctc ttcgtcgtgc ttgtgccaag
     5341 aatcgcagaa ggaaagatcg tcgaaatcga cgcagtcgcc gattccgggc acctgagacg
     5401 gctcgaactc tcggtgctga gcgattgact cgggcggtgc atcttcccga ttctgcggaa
     5461 tgctcacgca ggcttcatgt tcctgcgaac ccactctgtc agctcggctt cggagaaagc
     5521 gcccgaggcc atagagagca tgacgacaac gcactgcacg tcgtccgcat gaagctcgaa
     5581 tccattcaga agaagaataa gctcaagcgc cacgaaggcg atacgtttgt tgccgtccac
     5641 gaacgcatgg ttttttgcaa gcccgaatcc ataagcagca gccagttcgg ccgcgtccgg
     5701 ctcgccataa gccgcagtgt ttttcgggcg ggcaagggcg gactcgaaca gtccgcggtc
     5761 acgaatgccg ctcgctccgc cgtgttcctc cagctgctcg tcatgagctg cgagaagcac
     5821 atccggatcg atccatatcc agtcggtcac ttggcgagtt cgcgtagcgc atgccgacgc
     5881 tttttcataa tctttcgagc ggcctcaagc tgcactattc gttccgcgtc gtaggacgcg
     5941 acccgaaagc cgtccggcgc cggcgtgagc gttagtgcat cccccttgtc gaccttcaat
     6001 gccgccagcg cctccttggg caaagtcacg cccaaggaat tcccgatctg gatgaccttc
     6061 accgtataat acatgggggt tcgttccaac attcgttata acgctacatt agttcaggcg
     6121 ggcggctcgt gcaacctgcc ctcactggtg cggcggcggg tcgtccgagc caaaaagaca
     6181 gggcaatcca tgaatgtcat tgtagctccc cagcgaacag ggatgattga tcggcttggc
     6241 ctctctgaac cgttcggcga tcaccttggc ccagggcgaa ttggaatcgg gcggcggtcc
     6301 aaagcggacg cttttttcgc ggggcctggc aggcggcaga cacgccgcgc gttcctcggg
     6361 cggcaacgta tcgatcttca ggggatcgca gccgaacagc gcgcggccga cgccggagat
     6421 tgatcccggg cccggcggtg ctggcgtgag ttcgggcatg gaaagctcgg gggatgtcgg
     6481 cccctgcaaa ggcatgaccg gcgcaaccag atcgggacgc aacacgaccg gcgccggcgg
     6541 cttgcggatg acagccggga acgacaccac gatttctcga gccggattcc ttcccggcag
     6601 gcgtgaattc atcgcgaaga tgaacgccgt caaaagagcg aaatgcagga gaagcactgc
     6661 actcgctgaa cccagtctgc gcaaggcggc tcctgtcatc gcgcgttgcg ccgcttatgc
     6721 gtctgcccga tattccagaa ggtctccggg ctggcacgca agctctacgc agattttctc
     6781 cagcgtcgag aaccggacac cctttacctt gcccgatttc aacagcgaga gattggcctc
     6841 ggtgataccg atcagttcgg ccagttcctt cgaccgcatc ttccgcctgg ccagcatcac
     6901 gtcaagattg acgacgatca tcagacgaac cccgcattct cgtccgcgac gcgcctcgcc
     6961 tcagccatca cgcgtgagac aacccaggcg acgccggcga gaaaaagcac ctgcacgtcg
     7021 tccgaaccga tggttagttg cagttcgcca tgctgaccgg gcggatgatc gcgcgtcagg
     7081 aaatatgtga ttggcgcctc cataacaaac gcagcgagga cgttccagaa cagcgcggcc
     7141 gtgatccgac cgaacgcgcg cagagcgtcg gagccgaaaa cctggcctcg attgaagagg
     7201 acaagaaggc gcatcagcgc aacgagcccg aacgtggcaa tggtcgccgg gatcatcgcg
     7261 caggcgagcg caagtgcacg gtcgagcagc ggcactgccg cagtcagatg cgaaaggccg
     7321 tgccgatcca tgtcgagacc gagcggccgc gcccattggg gcgcaagaaa catgaatgcc
     7381 gttcccagtg gaacgatgaa aaccgccgcg gccgagaacc acgccatgat gcggctcgcc
     7441 cgcgcgagac ccttggacac gatcattgcc agcctccttc tgaacaggat tctgattgta
     7501 ttgtatcgtt ttattatcgc aatgcaataa ttcccaaaaa tcctcaacct atgctctgcg
     7561 ctctggcccg cgcattgcac ccgttcagca cgagccagat ttctggcgtc ctgaatcgtc
     7621 tcatttcgct ccaacttctc cggagcaatt cgcgggtgaa tgtcatgagt cggctgaatt
     7681 tcaatgtgaa caagtacgac aacagccctc gccgacgcag ctacaatccc cgcggcaagg
     7741 tcgcgttgag aaccccatta catctgtcgg gagtgtacct gttcgctgtc gcgaccgcga
     7801 tgttggtatc gttcgccatc gtctggtacg cgtagaacgc accagcgtcc gctattccgg
     7861 cgcgatggtg agtttgatgc cgtcgagggc cggcgtgcag acgatctggc acgagaggcg
     7921 cgaggtcggc tcgacggcca gcgccatgtc gagcatgtcc acttcagccg ccgaaggcgg
     7981 cgcgagcttc gcgtaccatt cgccatcgat gtaaacgtgg caggtcgcgc acgcgcaggc
     8041 gccgccgcat tcggcggcga ccggaagccc cgcatcgcgc aatatttcca tgacgctcca
     8101 gccgtcgcgg ccatcgagca cgtgttcccg cccgttgcgg tcggtcgcaa cgatcttcat
     8161 gccacgccca gcttcttctg caggctggag gaggaggtcg tgtactggaa acgcagcttc
     8221 tcgttcggcc ggcagatgtg aaacgccgcc tgcgccatca gggctgcttc gtggaacccg
     8281 caaagaatga gtttgagttt gcccgcatag gcgttgatgt cgccgattgc gaagatgccg
     8341 ggcgttgagc tttcgaactt cgccgtatcc acgggaacga gattctcgtg caggttcagt
     8401 ccgaattccg caatcggacc gagcttgatc gtcaggccaa agaagggaag gaacgtatcg
     8461 cattcatgcg tccactcctt cttgtcggca ccggaaagcg tcacggcttc caattggccg
     8521 cccgcgccgt ggagcgcctt cacgctcgca acgtgaaaca cgatcctttt ttcggctgcg
     8581 agttcgcgca tgcggttgac gctgtgctgc gcggcgcgga aaccgtcgcg gtggtgcacg
     8641 agcgtgagac tgccggcgag cggcgccaga ttgatcgtcc agtcgagggc cgaatcgcct
     8701 ccgcccgcga tcacgatgtt ccgtccacgg agcgattcca tcttgcgcac cgcgtagtgg
     8761 acgctcttgc cttcgtaggc gtcgatgccg gcgatcggcg gccgcttcgg cacgaagctg
     8821 cccgcccccg ccgcgatgac gacgaccggc gccacgattt cggcgcccgc gtcggtcgac
     8881 agcttccagc gtccgtcggc caacctctca agaccgctcg ccatctggct cagatggaat
     8941 ccggcgtgaa acggctttat ctggtccatg agccggtcgg tcagttcctg acccgatacg
     9001 accggaaggc cgggaatgtc gtagatcggc ttttcgggat agagctcggt gcattgcccg
     9061 cccggccggt cgagaatatc gaccagctcg gccttcagat ccagcagccc cagctcgaac
     9121 acggcgaaga gaccaatcgg ccctgcgccg aggattgcaa catcggtttc gatagccatt
     9181 ccacaactgc ttccgtgcgc gcggcttata agtggagatc gccggccgcg gcaagccgcc
     9241 cggcggcgcg ccgccttgcc gatcccggct cctaccggcc cagataagcg tcgataaacg
     9301 aactcacaac gtcggcgcag cgtttcattg cgtgaaaagc ctccaccgct cgccggggat
     9361 cggttgcggg atcgaccgat tccgcatcgg ccgcggcctc gccaagcgca aaagcgccta
     9421 taccgttcgc ggcgcccttc agcgaatgcg tgagctcgcg ccaggcggtg acgtcggacc
     9481 gttcgagcgc cgtttcgagg cgttccacca ttgccgcagt ctgagccgaa aaaagttcga
     9541 gcacttcggc atcgagccgg gcttcgccgc cagtgtaacg ggcgagatgg gcaagatcga
     9601 tcaattgcgg ttccgaagac atgcgactcg cctgggcgac acctcataat gagcatagcc
     9661 ggccaaaatg gcgttaacgg cgcctcgatg gttcagaacg gcacccatcc acccgggaac
     9721 ggcgaaacgc cgaatatccg cgcgtgggcg aacagggtcg ccagaaacaa aagtgcggcg
     9781 acccagaacc gccagccgaa actctcgccg aggtgcaact gaccccgacc ggcaattact
     9841 gccgcgaaag gaatgtttga tgttctggac gcgaacgcat cccacgccgc gccgagcttt
     9901 cgcttgcgct tggcgtcgat ggagaacgtg cccgccagcg acagcaagaa aaacgtgccg
     9961 aagaaaatca ccgacgcttc gtcaccattc gccgcaacgt gaaaacccga ccagatcact
    10021 acgccccaga gaaagggatg acgcgtgatg cgcaggacgc ccgaaatcgc gtcggattgc
    10081 gtggcggccg cgccacccac ctgcacgcgc gtcggactgg gca
//
LOCUS       DASZMC010000221         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1742981,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000221 DASZMC010000000
VERSION     DASZMC010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742981"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VG274_04405"
     CDS             <1..1201
                     /locus_tag="VG274_04405"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HWE05925.1"
                     /translation="ENKLPPVIHIAGTNGKGSTCAFSRAMLEAQGLKVHVYTSPHLVH
                     FHERIRVAGKIISEDELSATLDDCERANDGTPITFFEITTAAAFLAFSRHPADALVLE
                     VGLGGKYDATNVIAKPAMTIITPVGLDHQEFLGSQLTGIAAEKAGIIKPGVPVVVGPQ
                     EDTARDVIMRRADALSAPAFFAGQDFFAHAEHGRMVYQDNDVLMDLPLPRLTGPHQIE
                     NAATAIAGLRHANRGWGREDAIEYGLRHVEWPARLQRLSKGPLVGLAPQGSEVWLDGG
                     HNPHAAHALARALADLEERSPKPLFLICGMLKTKDAEGYFSAFRGLARHVTTLAIPGE
                     EASRGAGELYDAARAAGLNASPADDLDDAMMQVEAWSRTQRDESPPRIVICGSLYLAG
                     KVLAENS"
BASE COUNT          347 a          542 c          528 g          333 t
ORIGIN      
        1 cgaaaacaaa ctgccgccgg tcattcacat cgccggaacc aacggcaagg gttcgacctg
       61 tgcgttcagc cgcgcgatgc tggaagcaca agggttgaag gttcacgtct atacctcgcc
      121 gcatctggtt catttccacg agcgcatccg cgtcgcgggc aagatcatct ccgaggacga
      181 actctccgcg acgctcgacg attgcgagcg ggccaatgat ggtacgccga tcacgttctt
      241 cgagatcacc accgctgccg cgttcctcgc gttctcgcgt catcccgccg atgcgctggt
      301 tctcgaagtc ggacttggcg gcaaatacga cgcgacgaat gtgatcgcga agcctgccat
      361 gacgatcatc acgccggtcg gcctcgacca tcaggaattt ctgggcagcc agctcaccgg
      421 catcgcggcg gagaaggccg gcatcatcaa gccgggcgtt cccgttgtgg tcggtccgca
      481 ggaagatacc gcgcgcgatg tcatcatgcg ccgcgccgat gcgctgagtg ctcccgcgtt
      541 tttcgccggc caggatttct tcgcgcatgc cgaacatggc cgcatggtct atcaggacaa
      601 tgacgtgctg atggatctgc cattgccgcg cctcaccgga ccgcaccaga tcgaaaacgc
      661 cgccaccgcg atcgcggggc tgcgccatgc gaaccgaggc tggggccgag aagacgccat
      721 cgaatacgga ttgcgccatg tcgaatggcc ggcgcgtttg cagcgtttga gcaaaggccc
      781 gttggtcggg ctggcgccgc aaggatcgga agtgtggctc gatggcgggc acaatccgca
      841 cgccgcgcat gcgctcgccc gtgcgctcgc cgatctggaa gagcgttcgc ccaagccgct
      901 gtttctgatc tgcggcatgt tgaagacgaa ggacgccgaa gggtatttct cggcgttccg
      961 cggcctggcg cgccatgtga cgacgctcgc cattccgggc gaagaggcgt cgcgcggagc
     1021 aggggagttg tacgacgccg cgcgcgccgc cggcctcaac gccagccccg ccgacgatct
     1081 cgacgatgcg atgatgcaag tcgaagcgtg gtcgcgcacg cagcgcgatg aatcgccgcc
     1141 gcgcatcgtg atctgcggct cgctgtatct ggcgggaaaa gttctcgccg agaacagctg
     1201 agcgttcttc gactccataa aagaagggct ccggcgcaat tagcaccgaa gcccaacgat
     1261 gtttcgctac gcagcaggcc cgccgaaggc tgtctttcaa gccgacaccg cggagcactt
     1321 tcgcctccac cacggtttgc atgcggagcg cttcccgacg cgttcggatc tctcacctcc
     1381 gaagccggaa cacgctgtcc gcgcggggtc cacgcaagcc ttggcccctt acctgtctgt
     1441 tcttgcggtg aatctagttt ttcatctcgg ctctgcgctc cgtgccacgc agaacaaata
     1501 tagaacacaa aggggaataa caagcccgat ataggtattt aatctaaagg caacattcgg
     1561 gaaagatttg cgatgatgcg tgaaatcggg tcgctggaat acggcgacag gatacctatt
     1621 ccctaatgag gaagaggtgc cgcaagtttt gccgcaatag gtatcctgtc gccgtatttc
     1681 tcgggccgat gcaacgattc agcatcgatc tcgacgcgat tcagcgctcg gattcatgtc
     1741 gattgagacg
//
LOCUS       DASZMC010000222         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_377753,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000222 DASZMC010000000
VERSION     DASZMC010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377753"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..1328
                     /locus_tag="VG274_04410"
     CDS             456..1328
                     /locus_tag="VG274_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWE05926.1"
                     /translation="MKRAILTAAATLSIGLLSFGLAATSASAQETKCEPDKLATKYPS
                     LVGKTINLAQDGESPPFSFRDPKDFDHIVGMDTELAEAVFKCAGVPIKIVTAGWSGLL
                     PSVISGQTDVMWDILYYTPERAKKVDFVTYMVAATGALVAKGNPQNIHSMADACGKRA
                     TAGLATVEEAAFRDQAAKCIAAGKPDISIVTYPDIPGGTRLIQNDRADVMLSGLSVVN
                     ALVAQNPNAFELGYTIVTDYKVSAAVTRGNKDLAKLIYDGLKIAMADGSYKTIIEKYG
                     FSMSLALKPEILTQ"
     gene            1561..>1790
                     /locus_tag="VG274_04415"
     CDS             1561..>1790
                     /locus_tag="VG274_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWE05927.1"
                     /translation="MAAGALMGLGIALMRMSRFALVRAIAWFYIWFVRGTPQLLQLVF
                     LYDALPPLGIKLNAFVTAVVGFALNEAAFSAE"
BASE COUNT          342 a          545 c          529 g          374 t
ORIGIN      
        1 tcgcggccct ggcctgcgtg gccgcgcatc agatgcgcaa attattggcg cccggtcgta
       61 aagcgccaac tttacgagat atcgcaaagt gacgcatccc gtcgacgcgc aaacgcccgc
      121 tggcgccgcg cttattcggc actcacatgg cgttgggact ggtatcgtat tcggcgccga
      181 actgggagca gttccagcat agatctgaaa tcgcaattta ttttggccca gatctccgcc
      241 ggggcacgtc cctgatcgcg cgatgtcatt cttctgtagc ggccggcaca agcctatcgc
      301 acctccgggt ctcggcaata ggaatacgct ggcacatagt atgcagcgcc cacggcgatg
      361 tcgcgcagcg atatcgatcg atgtgtcctc ttcagttgcg tcatgacgcg atggttgagt
      421 gacagatccg catgtcgtcg caaaaggaaa tcggcatgaa aagagcgatt ttgactgcgg
      481 ccgccacctt gtccatcggc ttgttgagct tcgggctggc ggcgacgagc gcctccgccc
      541 aggaaacgaa gtgcgaaccg gataaactcg cgacaaaata tccaagcctc gtcggcaaga
      601 ctatcaacct ggcgcaggat ggcgagagcc ctcccttcag tttccgcgac cccaaggatt
      661 tcgaccacat cgttggaatg gacaccgaac tggccgaagc ggtgttcaaa tgcgccggtg
      721 ttcccatcaa gatcgtgact gcgggatggt ccggtctcct gccttccgtc atctccggcc
      781 agaccgatgt catgtgggat attctctatt acactccgga acgcgcaaag aaggtcgatt
      841 tcgtgactta tatggtggcg gcgaccgggg cgttggttgc gaagggcaat ccccagaaca
      901 ttcattcgat ggccgacgct tgcggcaagc gcgcgacggc gggcctcgct accgtggagg
      961 aggccgcgtt tcgcgaccaa gccgcgaaat gcatcgcggc gggcaagccc gatatctcga
     1021 ttgtcactta tcccgacatt cccggcggca cgcgtctcat tcagaacgat cgcgcggacg
     1081 tgatgctgtc cggtctcagc gtcgtcaacg cgcttgtcgc gcaaaacccg aacgcttttg
     1141 aactcggcta tacgatcgtt acggactata aggtctccgc cgcagtcacc agaggcaaca
     1201 aggatctagc gaagctgatc tacgacggtc tcaaaatcgc catggcggat ggttcctaca
     1261 aaacgatcat tgagaaatac ggcttcagca tgagcctcgc gctcaagccc gagatcttga
     1321 cccagtaagc cggccgagaa aatgcgtctg attgggtcgg ccatccacgc gtcttctggg
     1381 gtagtcggtg gtctggcggt catgggagcg gcagcggggg gggcggccgc tgcggaactc
     1441 gctcctgcgc cgcaattcgc ggctcccgcg acggaatggc tgtctcagat cctctcttac
     1501 gcggtcgcgc cgttcttgct tcgcggcgct cttctagcga tcgaaatcgc tgctctggcc
     1561 atggccgccg gcgcgctcat ggggcttggc atagccttga tgcggatgtc gcggttcgcg
     1621 ctcgttcgcg cgatcgcgtg gttctacatc tggttcgtgc ggggcacacc tcagcttctg
     1681 caactggtct ttctctacga cgcgctgccg ccgctcggca tcaagctgaa cgccttcgtc
     1741 accgccgtcg tcggcttcgc cctcaacgaa gccgccttta gcgcggagat
//
LOCUS       DASZMC010000223         3962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5311115,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000223 DASZMC010000000
VERSION     DASZMC010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5311115"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VG274_04420"
     CDS             complement(<1..365)
                     /locus_tag="VG274_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HWE05928.1"
                     /translation="MKNITCIEDLRRIARRKVPRAFFDYVEAGSYSEDTLRANRADLE
                     NIKLRQHVMVGVAERDTSTTILGQKVAMPLALAPVGMSGLQWGNGEILACRAARAAGV
                     PYTLSTLSISSLEHVAEAAG"
     gene            complement(382..795)
                     /locus_tag="VG274_04425"
     CDS             complement(382..795)
                     /locus_tag="VG274_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05929.1"
                     /translation="MPFVADASVAACWAFDDEAHPTAAFALERIRSDEARVPCLWWFE
                     VRNVLIVNERRRRISAADVSGFLRTLSRLAITVDHAPDETEILALARRRRLSVYDAAY
                     LELAKRESLVLATLDRELARAAGAEGVSLLKAREI"
     gene            complement(795..1040)
                     /locus_tag="VG274_04430"
     CDS             complement(795..1040)
                     /locus_tag="VG274_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWE05930.1"
                     /translation="MRTVQASAAKTHLPELLDDVERGETVIITRHGRAIARIVPEEAR
                     RQEEIDRAIETIKALRERTGKIALGDLLSARHEGHKY"
     gene            complement(1123..1512)
                     /locus_tag="VG274_04435"
     CDS             complement(1123..1512)
                     /locus_tag="VG274_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE05931.1"
                     /translation="MQIKLAAVTVENQEAALQFYTGRLGFTKKADISMGPYRWLTVSS
                     PDGLEGVELLLEAAAFPPSHVYQKARYDAGIPSTAFFTSDIASEFKRLKDAGVSFRSE
                     PRNTGPAISAVFDDTCGNLINLVQPPA"
     gene            complement(1666..2094)
                     /locus_tag="VG274_04440"
     CDS             complement(1666..2094)
                     /locus_tag="VG274_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05932.1"
                     /translation="MVGLNPTTWRALDFVDFVLLVVILFPGEPALSQAGGWWLPLVRM
                     RPMHRDYTREALLAAAESPHDPYCLTKKHHVPTDGLCTCHIGKARFALEIGSMSFAEN
                     AQLVADELLPAEKSGPVEGYDVAYRRALVKRTKKMAIKLD"
     gene            complement(2192..2905)
                     /locus_tag="VG274_04445"
     CDS             complement(2192..2905)
                     /locus_tag="VG274_04445"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWE05933.1"
                     /translation="MRSIIAVAVCALFASPAFGDDRALITTQMQEMADAVAPGNVAVW
                     DKYLDASVIYAEEDDSWKGKAGMLKEIRPLPKGLGGTIRIELLSYHEDGDVAVALFRQ
                     NETEHYFGQTIYAKYLTNTTWRKRAGGWKLIAAQVLAEKTDPPAIALPAGRLAQYAGT
                     YRLKDSEPTTTLVLKDGALIGTRNRRQPATWNAEAPDVFFVKGDPRIRQIFQRDAAGK
                     ITGFVERRESWDIVWIRIK"
     gene            complement(3014..3433)
                     /locus_tag="VG274_04450"
     CDS             complement(3014..3433)
                     /locus_tag="VG274_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05934.1"
                     /translation="MTLAVVDASVALKLFIAEVGSAEAEEIQRTHDLIAPDLVLPELL
                     NALWKARRLGLVGAEQARQIAAAIAQPFFAFVPSEELLDRAWAIAVALDHPAYDAFYI
                     ALAERERCVLFTADERLTRKTRRTSFTKLVKMLKTRR"
     gene            complement(3430..3666)
                     /locus_tag="VG274_04455"
     CDS             complement(3430..3666)
                     /locus_tag="VG274_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05935.1"
                     /translation="MGQVIIRNLEDGVLARLKKRAAGQGLSLEESLRRALAALAGPSK
                     DEVTAQLRRIRAMASVRTKKPFAEDLVREGRNER"
     gene            complement(3727..>3962)
                     /locus_tag="VG274_04460"
     CDS             complement(3727..>3962)
                     /locus_tag="VG274_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05936.1"
                     /translation="DRHIRKMRIWIDIPDRIANPLTEIGARQMRSRASVVREALNEYL
                     SRHGGSDPHAAFGLWGKRGRDGLVRQRQLRAEC"
BASE COUNT          709 a         1254 c         1227 g          772 t
ORIGIN      
        1 ccagcagctt ccgccacgtg ctcgagcgag gagatggaga gcgtgctcag cgtgtaggga
       61 acgcctgcgg cgcgggcggc gcggcaggca aggatttcgc cgttgcccca ttgcagtccg
      121 ctcatcccga ccggcgcgag cgcgagcggc atagccacct tctggcccag aatcgttgtc
      181 gaggtgtcgc gctcggcgac gcccaccatc acatgctggc gaagcttgat gttttccagg
      241 tccgcgcggt tggcgcgcaa cgtgtcctcg gaataggagc ccgcctcgac gtaatcgaag
      301 aaggcgcgcg gcaccttgcg ccgcgcaatc cgccgcagat cctcgatgca ggtgatgttc
      361 ttcatgtgcg ccaccgcgcg atcatatttc ccgcgccttc agtaatgaaa cgccttcagc
      421 acctgccgcc cgggcaagct cgcggtcgag ggttgcaagc acgaggcttt cgcgtttcgc
      481 cagctccagg taagcggcat cgtaaacgct gaggcggcgg cgtcgggcaa gcgcgagaat
      541 ttccgtttca tcgggcgcat gatcgaccgt tatggccaga cgggacagcg tgcgcaggaa
      601 cccggaaaca tccgctgcgg atattcgtcg tcggcgctca ttcacgatca gcacgttgcg
      661 cacctcgaac caccacaggc aaggcacgcg ggcctcatcg gagcggatgc gctccagcgc
      721 gaatgcggcg gtcggatggg cctcgtcgtc gaatgcccaa caggcggcga ccgaggcgtc
      781 agcgacaaag ggcatcagta tttgtggccc tcatgtcgcg cggacagcag atcgccgagc
      841 gcgatcttgc ccgtacgctc gcgcagcgcc ttgatggttt cgatggcgcg gtcgatctct
      901 tcctgccggc gcgcctcttc gggcacgatg cgggcgatgg cccggccgtg gcgtgtaatg
      961 atcactgtct ctccgcgctc tacgtcgtcg agcagctcgg gaagatgggt cttggcagca
     1021 gaagcctgaa ctgtgcgcat atttcgactg gtcgattcga ctggtcgtat catgcagcag
     1081 ggcgaccgtc cgtgcaagcc cgaaacgcgc gatggacgcg gatcaggccg ggggttgaac
     1141 caggttgatg aggtttccgc acgtatcgtc gaaaaccgcc gaaatggcag ggcccgtgtt
     1201 ccgcggctcg ctacggaagc tgacgccggc gtccttcagc cgcttgaact cgctcgcgat
     1261 atcgctggtg aagaaagcgg tggagggaat tccggcatcg tagcgggcct tctggtagac
     1321 gtgcgaaggc ggaaacgccg cagcctccag cagaagctcc acgccttcca gaccgtccgg
     1381 cgaactcacg gtcagccagc gatacgggcc catcgaaatg tcggccttct tcgtgaagcc
     1441 gagcctgccc gtatagaatt gcagcgcggc ttcctggttt tcgacggtca cggcagcgag
     1501 cttgatctgc atggtgtctc tccgaaggtt tgcgagccat cctgcaatcg tacggctctg
     1561 ctatcaagac gaacatcacg tcctcgaacc gacacggctc aggtggaatt tgctgccggt
     1621 ggaagtggcg aagagcacaa ccaacccccg ccgggacact cggccctagt ccagcttgat
     1681 tgccattttt ttagtgcgtt tgacgagtgc ccgccggtac gcaacatcat aaccttcaac
     1741 cggtcccgat ttctcggccg ggagcaattc gtcggcgacg agctgcgcat tttcggcgaa
     1801 cgacatgctg ccaatttcca gcgcgaatcg cgccttgccg atatggcagg tacaaagccc
     1861 atcggtggga acatgatgtt ttttggtgag gcaatatgga tcgtgcggac tctccgccgc
     1921 cgccagcagt gcctctcttg tgtagtcgcg atgcatcggg cgcattctta ccagcggcag
     1981 ccaccatccg ccagcttgtg aaagagcggg ctcgccgggg aaaaggatca ccacaaggag
     2041 cacaaagtcc acaaagtcca atgcgcgcca cgtggtcggg tttaaaccga ccattccgcg
     2101 ctactcatct tttgcgcctt ctttgtgctc ttcgtgatct tcgtggttat aactttcctt
     2161 cggcgcctcc gcgcctccgc gaggcgctgc attacttgat cctgatccac acaatatccc
     2221 agctttcgcg ccgctcgacg aagccggtga tcttgcccgc ggcgtcgcgc tggaagatct
     2281 ggcggatgcg cggatcgccc ttgacgaaga acacgtcggg cgcttccgca ttccatgtcg
     2341 ccggctggcg gcggttgcgc gtgccgatga gcgctccatc cttaagcaca agcgtcgtgg
     2401 tcggctccga atccttgagc cggtaggtgc ccgcatattg cgcaagccgg cccgcgggga
     2461 gcgcaatcgc ggggggatct gtcttctcgg ccagcacctg cgccgcaatc agtttccagc
     2521 cgccggcacg ctttctccac gtcgtgttgg tgagatattt tgcataaatg gtctggccga
     2581 aataatgctc ggtctcgttc tggcggaaga gcgccacggc cacgtcgccg tcttcgtggt
     2641 aggacaaaag ctcgatcctg atcgtgccgc caagcccctt cggcagcggc ctgatctcct
     2701 tgagcatccc cgccttgccc ttccaggaat cgtcctcctc ggcatagatc acgctggcgt
     2761 cgagatattt gtcccacacc gccacgttgc cgggcgcgac cgcatcggcc atctcctgca
     2821 tctgcgtggt gataagcgcg cggtcgtcgc cgaacgccgg cgacgcgaac aaggcgcaga
     2881 cggccaccgc gattatcgaa cgcatcaaat cccccgcccg caacggcgcg ctcgggtcag
     2941 attacgcgat tgccttgcca tgacagttca agtccgcgtg atcggtgcgg tgactcggca
     3001 acaggacgat ggatcaacgc cgagttttca gcatcttcac cagcttcgtg aaactggtgc
     3061 ggcgcgtttt gcgggtcagc cgctcgtcgg cggtgaacag gacgcatcgt tctctttcgg
     3121 ccagcgcgat ataaaaggcg tcgtaggcgg gatggtcgag tgcgacggca atcgcccagg
     3181 cccgatcgag aagttcctcc gacgggacga acgcaaagaa gggctgtgcg atggcggccg
     3241 cgatctgcct cgcctgttcc gctccgacga ggccaagccg ccgcgccttc cagagggcgt
     3301 tgagaagttc cgggagcacg aggtctggtg cgatgaggtc gtgcgtgcgc tggatttcct
     3361 cggcttccgc actccctact tcggcaatga acagcttcaa tgcgacgctt gcatccacga
     3421 ccgcaagcgt caccgttcgt tgcgcccctc gcgcacgagg tcctcggcga agggcttttt
     3481 cgtccgcacg gacgccatcg cccgaatgcg gcgcagctgc gcggtgacct catccttgga
     3541 aggcccggca agggcggcaa gggcgcgccg cagcgattcc tcgagcgaca ggccctgccc
     3601 ggcggcacgc ttcttgagcc gcgccagaac accgtcttca agattgcgta tgatgacctg
     3661 gcccatgggc gggactccgc atgctatcat gacgatagca gaagggaggg cctgtgtcac
     3721 ggcagttcaa cattcggcgc ggagctggcg ctgccggacg aggccgtcgc gcccgcgctt
     3781 gccccacagg ccgaaggccg cgtgcgggtc gcttccgccg tgccgcgaaa gatattcgtt
     3841 caaggcttcc cgcacgacgc ttgcgcgcga tctcatttgc ctcgcgccta tctcggtcaa
     3901 cgggttggcg attcgatcgg gaatgtcgat ccaaatgcgc atctttcgga tatgtcggtc
     3961 ag
//
LOCUS       DASZMC010000224         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3399927,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000224 DASZMC010000000
VERSION     DASZMC010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3399927"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1033
                     /locus_tag="VG274_04465"
     CDS             32..1033
                     /locus_tag="VG274_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWE05937.1"
                     /translation="MGASRGDGGRRAFLARGVAALLAAALPAHADANSNFPDRPVQVY
                     VPFAAGSASDVITRILLGRMTTSLGQNFVVENRPGAGGNTGTAAAAHAAPDGYTLVMS
                     TSGPLAANKALFRELGYDPQNDFAPICLFATLPNIVVINAKLPPKTLRELIDYAKQHP
                     KQLNYGSVGVGSSQHLAGAYFEQLTGTQFVHVPYRNIGSYAPDFISGQVPVGFQLLPN
                     VLGLIKNGDARPLAVAASKRMAALPDVPTAAEAGLPGYESAAWLALLAPANTDKAIVD
                     RLYAAAEEATKDPKLRALFTEQGAEPADLGPDDLKKFMASEISKWSSVIGKLGIAPM"
     gene            1152..>1511
                     /locus_tag="VG274_04470"
     CDS             1152..>1511
                     /locus_tag="VG274_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05938.1"
                     /translation="MPKFLRIGVHQSNVSGYTSKAWCVRRVGSTIFLKWGAVEVHGVG
                     DGRKVYWTLPPRDKAIRCGTVQRAQDYAKAAIARRRNHRYELLTGSIATRRRTADRDA
                     ELEQALATILIIDIVRST"
BASE COUNT          295 a          477 c          463 g          276 t
ORIGIN      
        1 cgacgcgatt ctccgggcat aggtagtcgc gatgggtgca tctcgcgggg acggcggccg
       61 cagggcattt ctggcgcgcg gtgtggctgc gctgttggcg gccgcgctgc cggcccacgc
      121 cgacgcgaac tcgaattttc ccgaccggcc tgtccaggtc tatgtgccat tcgccgccgg
      181 cagcgccagc gacgtcatca cgcgaattct gctcggccgg atgacgacat cgctgggcca
      241 gaatttcgtg gtcgaaaatc gccccggcgc gggaggcaac accggcacgg cagccgccgc
      301 gcacgccgcg ccggacggct acacgctggt gatgagtacg tccgggccgc tggctgcgaa
      361 caaggcgcta ttcagggaac tcggttacga tccgcaaaac gacttcgccc cgatctgttt
      421 gttcgccacg cttcccaata tcgtcgtgat caacgcgaaa cttccgccca aaacactgcg
      481 cgagctgatc gattatgcga agcaacatcc gaagcagctc aattacggct cggtcggcgt
      541 gggctcctcg caacatctgg ccggcgccta tttcgaacag ctcaccggaa cgcagttcgt
      601 ccacgtcccc tacaggaata tcgggagcta tgcgcccgac ttcatctccg gccaggtgcc
      661 ggtcggattt caactgctgc cgaacgttct gggcctgatc aagaacgggg atgcgcgccc
      721 gcttgcggtg gccgccagca agcgtatggc ggcattgccc gacgtgccga cggcggccga
      781 ggccggcctg ccgggttatg agagtgctgc atggcttgcg ttgctggcgc ccgccaacac
      841 cgacaaggct atcgtcgata gattgtacgc cgccgccgag gaggccacga aagatccgaa
      901 attgcgtgcg ctgtttacag agcagggcgc cgaaccggcg gacctcggtc cggacgacct
      961 gaaaaaattc atggcatcgg agatttcaaa atggtcctcc gtcattggaa aattgggcat
     1021 tgctccgatg taggcacaac agccccgtcg gctccgcccc gccttacaag ctcgctgttg
     1081 ccaacgcagg agtgtgtgac tacgctttac cccaaatagg ccaaaacggt ccctggatcg
     1141 gccgcacgtt catgccaaaa ttcctgcgca tcggagtcca tcagtcgaac gtttccggat
     1201 acacgtcaaa ggcatggtgc gttcgacggg ttggctcgac gattttcctg aagtggggcg
     1261 ccgtggaggt ccatggcgtc ggagatgggc gaaaggtcta ctggacgctt ccaccgaggg
     1321 ataaagcaat tcgttgcggt acggtgcagc gtgcccagga ctatgcgaag gccgcgatcg
     1381 cacggcgacg caaccatcgc tatgaactgc tgactggaag cattgcgacc cggcgccgaa
     1441 ccgccgatcg cgacgccgag ctcgaacaag cgctcgccac gatcctcatt atcgacattg
     1501 tccggtccac c
//
LOCUS       DASZMC010000225         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2294141,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000225 DASZMC010000000
VERSION     DASZMC010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2294141"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VG274_04475"
     CDS             <1..456
                     /locus_tag="VG274_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05939.1"
                     /translation="TNAPQVNSVQYLDTGVILQVTPRVNRGGQVMMDIDQEVAEPTST
                     TSSSINSPTIQQRKIVSSVSVLDGETIALGGLFSNQVSKTKGGIPFLQDIPYLGHLFT
                     NTGDSVAKTELMVLITPHVVTDPKRALSVTDELRRKLPDVQPLFLQQGK"
     gene            470..1357
                     /locus_tag="VG274_04480"
     CDS             470..1357
                     /locus_tag="VG274_04480"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05940.1"
                     /translation="MTRARERGWALISVLWTVAMLAMMAAAAQELTLTSVRTERRALV
                     RAHLDADLDAAIVRALLALQDSRQADRWRVDGYPYKFSYEGVDMTVSVQDEEGKFDLN
                     ETDGEDLLPLFESVGLDSRTASVITDRIVEWRTEPGSDDAHTLHGGTDQDYAAAGRPW
                     RPRHGDFQTVSELNLVLGMTPQLFAKVRSALTVYSRNDSPDPNVAPRVVLAALNPGNP
                     GQVDKIMAQRSGAFTQVGLSTTQSTSGGATIAPGSDLGGRMFEIDVDASYAGRHATRR
                     VVLVMTGDTARPYLVQAWE"
     gene            1443..1799
                     /locus_tag="VG274_04485"
     CDS             1443..1799
                     /locus_tag="VG274_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05941.1"
                     /translation="MTEAAWPRVSARVAAIALLAALAAACGPQNNRNEKLAHEGRHGH
                     GLRVACADDIEKYCANADRRRRCLKQNIDKVSDACKTALAQRRNKGGNDEGANDKTNN
                     NKTNNTNNNSDDDDNN"
     gene            complement(1963..2535)
                     /locus_tag="VG274_04490"
     CDS             complement(1963..2535)
                     /locus_tag="VG274_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HWE05942.1"
                     /translation="MMNFLRAFRPVGEDFLSTIVFVAVYVAAGNIYAGIGAGMATGFV
                     QIAALKWRGRRIDLMQWASVALVVVLGSATLLTHDPRFFMVKPTIGAFAIATVMLRPN
                     WMARYMPPAVTANISPRTPLIWGYVWSVTIFALGIANLFVAFVYGPNIWAWFTAFVPI
                     SVQLSLFLAQYALLRHAVISTIRSRASAAA"
     gene            complement(2532..>3361)
                     /locus_tag="VG274_04495"
     CDS             complement(2532..>3361)
                     /locus_tag="VG274_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWE05943.1"
                     /translation="DQIAYGVVAADGTISRLDMFAAPYASMIHDFFVTQSHVAFPVLP
                     LAGNLERAMNGGPPFAWEPERGSHIGLMRRDAVVETMRWFTTDPCYVFHPMNMWEDGD
                     RLYADVMQYENPPLFPTADGSPGKKATARLARWTFDLAGRTDTIAREYIDDMEGEFPR
                     FDGRRAGLSYRHGYFAASARGATDISFDSIAHIDVRSGRRQVYTFPSGDSPGEPVFVP
                     RSDAEGDGWLLAVVYRGGENRSDIAVFDAMDVARGPVGTACLPRRVPFGFHGNWVPR"
BASE COUNT          682 a         1094 c         1060 g          525 t
ORIGIN      
        1 accaacgcgc cgcaggtgaa cagcgtccaa tatctcgaca cgggcgtgat cctgcaggtg
       61 acgccgcgcg tcaaccgcgg cggccaggtg atgatggata tcgaccagga agtggccgag
      121 ccgacctcga ccacaagctc gagcatcaac tcgcccacca tccagcaacg caagatcgtc
      181 agctcggttt ccgtgctcga cggcgagacg attgcgctgg gcggcctgtt cagcaatcag
      241 gtcagcaaga cgaagggcgg catcccgttc ctgcaggaca ttccctatct cggtcacctg
      301 ttcaccaaca ccggcgacag cgtcgccaag accgagctga tggtattgat cacaccgcat
      361 gtcgtgacag acccgaagcg ggcgctctcg gtgaccgacg agcttcgccg caagcttccc
      421 gatgtgcagc cgcttttcct gcagcaggga aaatgataac gggatgcgca tgacacgcgc
      481 tcgcgaacgc ggctgggcgc tcatctcggt tctgtggaca gtggcgatgc tcgccatgat
      541 ggccgccgcc gcccaggagc tgacgctcac ctctgtgcgc accgagcgca gggcgctcgt
      601 ccgcgcccac ctcgacgccg atctcgacgc cgccattgtg cgcgcgctgc tcgcgctcca
      661 ggattcgcgt caggccgatc gctggcgcgt cgacggatac ccctacaaat tctcctacga
      721 gggcgtcgac atgaccgtct cggtgcagga cgaggagggg aagttcgatc tcaacgagac
      781 cgacggggaa gatttgctgc cgctcttcga atcggtcggc ctcgattcca ggacagccag
      841 cgtgatcacc gatcgcatcg tcgagtggcg cacggagccc ggaagcgacg acgcccacac
      901 gctccacggc ggcaccgacc aggattatgc cgccgccggc cgcccctggc gcccccggca
      961 cggcgatttc cagaccgtct cggaactcaa tctcgtcctt ggcatgacgc cgcagctgtt
     1021 cgcgaaagtg cgctccgcgc tcacagtgta ttcgcgcaac gacagccccg atcccaatgt
     1081 ggcgccgcgc gtggtcctcg ccgcgctcaa tcccggcaat ccggggcagg tggacaagat
     1141 catggcccag cgctccggcg cgttcacgca ggttggtctg tcgacgaccc agagcacgtc
     1201 cggcggcgcc acgatcgcgc cgggatcgga tcttggcgga cgcatgttcg aaatcgacgt
     1261 cgacgcaagc tacgccggcc ggcacgcgac ccgtcgggtc gttctggtga tgacgggcga
     1321 caccgcacgg ccctacctcg ttcaggcgtg ggagtaggcc gcgggttgac gccagaacgg
     1381 ttctgcggtt gcatcgcgcg caccgtgctt cgaaggctca atccacgagg acgaaaatgc
     1441 gcatgacgga agcggcatgg ccgcgcgtgt cggcacgagt cgctgcgatt gccctgcttg
     1501 ccgccctggc cgcggcctgt gggccgcaga acaaccgcaa cgagaaactc gcgcatgagg
     1561 gccgccacgg gcacgggttg cgcgtggcgt gcgccgacga tatcgagaaa tattgcgcca
     1621 acgccgacag gagacggcgg tgtctgaagc aaaacatcga caaggtcagc gacgcctgca
     1681 agaccgctct cgcccaacgc cgcaacaagg gcggcaacga cgagggcgcc aacgacaaga
     1741 ctaacaacaa caagacgaac aacacgaaca acaacagcga cgacgatgat aacaattagg
     1801 gcgcgttgaa gcggaaagcc cgcgccgccg gacgttggcc gcctgccata atcaaattat
     1861 gtcattcccg ctccgccgcc agcgcattgg ttgatgtgca gataccttag cgcttcggcg
     1921 ggaatgacat gcctggtcgc gggagaacaa ttggtctcga ccctacgccg ccgcgcttgc
     1981 gcgggagcgg atcgtactta tgaccgcgtg gcgaagcagc gcgtattgcg ccagaaacag
     2041 cgacagctgc accgagatgg gcacgaaggc ggtaaaccac gcccagatgt tcggaccgta
     2101 gacgaaggcg acgaacagat tcgcaatgcc cagcgcgaag atcgtgaccg accacacgta
     2161 gccccagatg agcggcgtac gcggggaaat gtttgctgtc accgcaggtg gcatatagcg
     2221 cgccatccag ttgggccgca gcatgacggt tgcgatggcg aatgcgccga tggtcggctt
     2281 gaccatgaag aagcgcggat cgtgcgtcag caatgtcgcc gaaccgagca ccacgacgag
     2341 agccacgctc gcccattgca tgagatctat tcggcgcccg cgccatttga gcgcggcgat
     2401 ctgcacgaag cctgtcgcca tgccggcgcc gatgccggca tagatgtttc ccgcggcgac
     2461 gtagaccgcg acgaaaacga tcgtggagag aaaatcctcc ccgacgggcc gaaacgccct
     2521 caggaaattc atcatcgcgg cacccagttg ccgtgaaatc cgaacggcac gcgccgcggc
     2581 agacacgcgg tgccgacagg ccctctcgcc acgtccatgg cgtcgaagac cgcgatgtcg
     2641 cttcggtttt cgccgccgcg atagacgacg gcaagcagcc agccatcgcc ctccgcatcg
     2701 ctgcgcggaa caaacacggg ttcgcccgga gaatctcccg aaggaaacgt atagacctgc
     2761 ctgcgcccgg aacgcacatc gatatgggcg atggaatcga agctgatatc ggttgcgccg
     2821 cgcgcgctgg ccgcgaaata gccgtgcctg tagctgaggc cggcgcggcg accgtcgaag
     2881 cgcggaaact cgccctccat gtcgtcgatg tattcgcgcg cgatcgtgtc ggtccggccc
     2941 gcgagatcga aggtccagcg cgcgagccgg gcggttgcct tcttgcccgg cgatccatcc
     3001 gcagtgggaa acagcggcgg gttttcgtac tgcatgacgt cggcgtagag cctgtcgccg
     3061 tcctcccaca tgttcatcgg atggaacacg tagcagggat cggtggtgaa ccagcgcatc
     3121 gtttcaacta cggcgtcccg gcgcatgagg ccgatatgcg agcccctttc gggctcccag
     3181 gcgaaggggg gcccgccgtt catcgcgcgc tcgaggtttc ccgcgagcgg cagcaccgga
     3241 aaagcgacat ggctctgcgt cacgaaaaaa tcgtggatca tgctcgcata gggtgcggca
     3301 aacatgtcga gacgcgaaat cgtgccgtcg gctgcgacga cgccataggc aatctggtcc
     3361 g
//
LOCUS       DASZMC010000226         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2849111,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000226 DASZMC010000000
VERSION     DASZMC010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2849111"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VG274_04500"
     CDS             <1..572
                     /locus_tag="VG274_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWE05944.1"
                     /translation="TFLFDGTIRENVMFSRPHATEAEVLNACRIARVDEFAEVLEQGY
                     DTVVGERGVKLSGGQRQRLSIARAILADPRILILDEATSSLDSESEAMIQQGLAHLMK
                     GRTTFVIAHRLSTIRRADQILVIEGGRIVERGTHSELYALRGRYWDMYTRQHALDENL
                     FLAPGEGDAVPEPVASGPVREGPLLPRAL"
     gene            925..1716
                     /locus_tag="VG274_04505"
     CDS             925..1716
                     /locus_tag="VG274_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE05945.1"
                     /translation="MKKPLLATIFAVSLAVSPLLAQLPAPNAAGTSAGHDIFSFHDID
                     AANRFWNALGGQPAELGPLKMTKYPGVLFLMRKGDPKGGTEGSTIDYIGFKVRDLKGT
                     LARLAEAGSMPTPGGSATQVFVMGPDDVKVRITEDRSLATPIAADMIRMMIPDVEAAQ
                     AWYEKWFGAKPVKHGNETVSEIPGANILFAKATGPVAGTKGRAFDRIGLEVRNVEQKC
                     KDLEAGGIKLDAGYRKAQNMNLAVCVLTDPWGTYIEISEGLAAVK"
     gene            complement(1723..>2070)
                     /gene="aceE"
                     /locus_tag="VG274_04510"
     CDS             complement(1723..>2070)
                     /gene="aceE"
                     /locus_tag="VG274_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWE05946.1"
                     /translation="RWNRLHPAEPAKVPYIQQVLEGVEGPIVAASDSMKVVQDQIAPW
                     LPGRYVALGTDGFGRSDNRAHLRKHFEVDAASIAAAALSRLARDGKFDAKRAAKAIGE
                     LGIDPEKVDSAVA"
BASE COUNT          400 a          642 c          645 g          383 t
ORIGIN      
        1 agacgttcct tttcgacggc acgatccgcg agaatgtgat gttttcgcgt ccgcatgcta
       61 ccgaagccga ggtgctcaac gcgtgccgca tcgcgcgcgt cgacgagttc gccgaagtgc
      121 tggagcaggg ctacgacacc gtggtcggcg agcgtggcgt caagctctcc ggcggccagc
      181 ggcagaggct ttcgatcgcg cgcgccatcc tcgccgatcc gcgcattttg attctggatg
      241 aagcgacgtc gagcctcgat tcggagtccg aagccatgat ccagcagggt ctggcgcatc
      301 tcatgaaagg gcggaccacc ttcgtgatcg cccaccggct ttccacgatc cggcgcgccg
      361 atcagatcct ggtgatcgaa ggcggccgca ttgtcgagcg cggcacacac agcgagctct
      421 acgcgctgcg cggccgctac tgggacatgt atacccggca gcatgcgctc gacgagaatc
      481 tgttcctcgc tcccggcgaa ggagacgcgg tcccggagcc ggtcgcgtcg ggaccggtcc
      541 gcgagggccc cctgctgcca cgcgcgctgt gacatgtggg gccaggttgg taacctgcgg
      601 ccgattgtca atctaatgcc atgtagtttt ttgggcccgg tgtctgagat gcagaggctg
      661 tcagaaaaaa cgatgcaaag tacgatggtg gaacaggctt cagcctgtgt tttcaatgcg
      721 cggcgaccgg ctgaagcctg tcccaccatc gtgcaggcct gcagcatccc gaagaatctg
      781 aaccatatcc ggcgggtgca taaaacaatt caatcggcct ggacgtttgc aaatcacagc
      841 accagcgcga ttaccaaatg tcggtagtgg tgcagttcga atcagcggcc gccggcccgt
      901 cgaggtgata tcatcgcccg aagtatgaaa aagccgcttc tcgcgaccat cttcgcagtc
      961 tccctcgcag tctctccttt gctcgcgcag ctcccggcgc ccaatgcagc gggcacatcg
     1021 gccggtcatg atatcttctc cttccacgac attgacgccg cgaacaggtt ctggaacgcg
     1081 ctgggcggtc agccggcgga gcttggtccg ctgaagatga cgaagtatcc aggcgtgctg
     1141 ttcctgatgc gcaagggcga tccgaaaggc ggcaccgaag gatcgacgat cgactacatc
     1201 gggttcaagg tgcgcgattt gaagggcacg ctcgcgcggc tggcggaagc gggaagcatg
     1261 ccaacgcctg gcggctccgc gacacaggtg ttcgtgatgg gtccggacga tgtgaaggtc
     1321 cgcatcacgg aagaccgctc gctcgcgacg cccattgcgg cggacatgat caggatgatg
     1381 attcccgacg tcgaagccgc gcaggcgtgg tacgaaaaat ggttcggcgc gaagcccgtg
     1441 aagcacggca acgagacggt tagcgagatt ccgggcgcca atattctgtt cgctaaagcg
     1501 acgggtccag tggcaggcac gaaagggcgc gccttcgacc gcatcggcct tgaagtgagg
     1561 aacgtcgagc agaagtgcaa ggatctcgaa gcgggcggta tcaagctcga tgccggctat
     1621 cgcaaggcgc agaatatgaa tctcgcggtc tgcgtattga ccgatccctg gggcacgtat
     1681 atcgagatca gcgagggtct cgccgccgtg aagtgagcgc gatcaggcga ccgcggaatc
     1741 cactttctcg ggatcgattc ccaattcccc gatcgctttc gccgctctct tcgcgtcgaa
     1801 cttcccatcg cgcgccagtc gcgacagcgc cgccgccgca attgacgccg catccacctc
     1861 gaagtgcttc ctcagatgcg cgcgattatc gctgcgcccg aagccgtcgg tgccaagcgc
     1921 cacgtagcgc ccaggaagcc atggcgcgat ctgatcctgc actactttca tcgagtccga
     1981 cgccgcgacg atcggtccct cgacgccttc cagcacctgc tggatgtacg ggaccttcgc
     2041 cggttccgcc ggatgcagcc ggttccaccg
//
LOCUS       DASZMC010000227         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1482087,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000227 DASZMC010000000
VERSION     DASZMC010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482087"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="hutI"
                     /locus_tag="VG274_04515"
     CDS             complement(<1..812)
                     /gene="hutI"
                     /locus_tag="VG274_04515"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HWE05947.1"
                     /translation="MRCDTLWHNARLLTLAPGRPGLGAVERGEIAATDGRIVFAGASD
                     DAPGFDAARRIDCAGRWISPGLIDCHTHLVYGGDRAREFEQRLAGASYEEIARAGGGI
                     ISTVKATREASEDELLDSALPRIDALIAEGVTTIEIKSGYGLTFDDELKQLAVAKRIA
                     EARRMDVAATYLAAHALPPEAQDNHDAFVDHVSREWIPRIARTGLADAVDGFCETIAF
                     SPEQIARVFAAAAAHGLRVKLHADQLSDLGGAALAARFHALSADHLEWSSEAG"
     gene            1098..1907
                     /gene="hutF"
                     /locus_tag="VG274_04520"
     CDS             1098..1907
                     /gene="hutF"
                     /locus_tag="VG274_04520"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050416 - formimidoylglutamate deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HWE05948.1"
                     /translation="MLWFETALLPQGWAKGVRVETQDGRIARVETDAQPNPGDERHAI
                     AIPGLANVHSHAFQRGMAGLAETRGPAHDDFWTWREIMYGFLDRLTPEDVEAIAALAY
                     VEMLERGFTRVGEFHYLHHDPSGVPYAELGEMASRIALAALETGIGLTLLAVFYAHGN
                     FGGAPASRGQRRFLNDVESFARLVEASRRAIARLPDANLGVAPHSLRAVAPDELREIV
                     PFARGGPVHIHAAEQTKEVEDCLAWCGARPVEWLLDHADVDRSWCLVHART"
     gene            1904..2443
                     /locus_tag="VG274_04525"
     CDS             1904..2443
                     /locus_tag="VG274_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE05949.1"
                     /translation="MTAQETARLAASGAVAGLCPVTEANLGDGVFPAREYLDAHGGFG
                     IGTDSNVYISAPEELRMLEYAQRLTRRARNLWPDAQGRSTGRALFDGALAGGAQALGQ
                     AIAGIREGASADLVSLDANDPALAGRIGDSILDSRIFAGAAIDCVWRHGEKRVSGGRH
                     HTRDEISARYRRTLERLLA"
     gene            complement(2490..2807)
                     /locus_tag="VG274_04530"
     CDS             complement(2490..2807)
                     /locus_tag="VG274_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HWE05950.1"
                     /translation="MNLRQFDVFPNPSSRTRRSRPYFVVLQSDQLHQLDTRIVAPLVA
                     PAKILHFEPLMPEVRVRDRPYVILAQELGAFPVQLAARAVANLESERYRIVSALDLLF
                     TGI"
     gene            complement(2804..3106)
                     /locus_tag="VG274_04535"
     CDS             complement(2804..3106)
                     /locus_tag="VG274_04535"
                     /inference="COORDINATES: protein motif:HMM:NF019009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HWE05951.1"
                     /translation="MPGHVSDPPKPFRSSARTRAERTGAHAARRRSANVSIDADVLRE
                     AKAMKINLSRTLEDALRGLLREEQLRRFDEDHREAFQSYNEFIAENGLWGEEYRKW"
     gene            3272..>4176
                     /locus_tag="VG274_04540"
     CDS             3272..>4176
                     /locus_tag="VG274_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWE05952.1"
                     /translation="MRNLAPAVKIASPAALRLERSGRGARLVLGGDWIVAEAARVDKE
                     LRALSFGGANELAIDGAAIELLDSAGAWLILRTKRDLEAKHATVQGPALPERYRALFD
                     KMERESLAPPVTHPHPLNFEEFLARIGRGTMHVLRQGYDMVGFLGRIAVETSEAVVAP
                     RRELPFPAFIQQIEETGLTALPIVGMLAFLLGVVLAYQGADQLKRFGAQIYTIDFLGV
                     GFLRELGGLVTAIIIAGRSGSAFTAQIGTMRVNEETDAMQTIGLNIAEILVLPRVLGL
                     VVALPLLTFFADVVGIVGGMVMTYF"
BASE COUNT          697 a         1369 c         1375 g          735 t
ORIGIN      
        1 cccgcttcgc tgctccattc gagatgatcg gcggacagcg catgaaagcg cgccgccagc
       61 gccgcgccgc ccagatcgga gagctgatcg gcgtggagtt tcacgcgcag cccatgcgcc
      121 gcggccgcgg cgaacacgcg cgcaatctgc tccggcgaaa aggcgatggt ttcgcagaat
      181 ccgtccaccg cgtcggcgag gccggtgcgc gcaatccggg gaatccattc ccggctcaca
      241 tgatcgacga acgcgtcgtg gttgtcctgc gcttcgggcg gcagcgcgtg cgcggcaaga
      301 taggtcgccg cgacatccat gcggcgcgcc tccgcgatcc gtttcgccac cgcgagctgc
      361 ttgagctcat cgtcgaaggt gaggccgtag ccggatttga tctcgatggt ggtgacgcct
      421 tccgcgatca gcgcgtcgat ccgcggcagc gccgaatcga gcagctcgtc ctcgctcgcc
      481 tcgcgcgtcg ccttgacggt ggaaattatt cccccgccgg cgcgcgcgat ctcctcgtag
      541 ctcgcgcccg cgagcctttg ctcgaattcc cgcgcccggt cgccgccgta gacgagatgg
      601 gtgtggcaat cgatcaggcc cggtgagatc cagcgcccgg cgcaatcgat gcgccgcgcc
      661 gcgtcgaacc ccggtgcgtc gtcgctggcg cccgcgaaca cgatgcggcc atccgttgcg
      721 gcgatctcgc cgcgttcgac cgcgccgagg ccgggccgtc cgggcgccag cgtcaacagc
      781 cgcgcattgt gccaaagcgt gtcgcagcgc atcgcacctc gaggacccgt ttgagggacc
      841 cgcttggcca agcgggtggg tacttcttct tacagcaatc actgcagatc ttaacccaat
      901 ttgttgtcat ggcccgcgca agcgggccac ccagccgtgt cctgacgacg cttggtaatg
      961 gaaaaaggaa ataacgcgat acttgcagca gtcgattctt ttcaactggg cggcccgctc
     1021 caggcgggcc gtgacaaaat tgggaaatgg gaacgttggt gtaaggtccg cagaagaaac
     1081 tttgcaacgg gacctgaatg ctctggtttg agaccgctct tctgccccag ggttgggcga
     1141 agggggtgcg tgttgaaacg caggacgggc gcatcgcgcg cgtcgaaacc gatgcacagc
     1201 cgaaccccgg agacgagcgt catgccatcg cgattccggg ccttgccaat gtgcactctc
     1261 acgccttcca gcgcggcatg gcggggctcg ccgaaacgcg cggtcccgct cacgacgatt
     1321 tctggacctg gcgcgaaatc atgtacggct tcctcgaccg gctcacgccc gaggatgtcg
     1381 aggcgattgc ggcgctcgcc tatgtcgaga tgctggagcg cggcttcacc cgcgtcggcg
     1441 aattccacta tctgcaccac gatccatcgg gcgtgcccta cgccgagctc ggcgagatgg
     1501 cttcgcgcat tgcgctagcg gcgctggaga cgggcatcgg actgaccctg cttgccgttt
     1561 tctacgcgca tgggaatttc ggcggcgcgc cggcgagccg gggccagcgg cgattcctga
     1621 acgacgtcga atccttcgcg cgtctcgtgg aagcgagccg ccgcgcgatc gcgcgattgc
     1681 cggacgccaa tctcggcgtg gcgccgcaca gtctccgcgc ggtggcgccg gacgaactgc
     1741 gtgaaatcgt tccgttcgcc aggggcggcc cggttcacat ccacgctgcc gaacagacga
     1801 aggaggtgga agattgcctc gcctggtgtg gcgcccgccc cgtcgaatgg ctgctcgatc
     1861 acgccgatgt cgatcggtcg tggtgcctgg tacatgcacg cacatgactg cgcaggaaac
     1921 cgcacgcctc gccgcgagcg gcgcagttgc ggggctctgt cccgtgaccg aagccaatct
     1981 cggggacggc gtatttccgg cgagagaata tctcgatgcg catggcggtt tcggaattgg
     2041 caccgattcg aatgtgtaca tctcggcgcc cgaagagctg cgcatgctcg aatatgcgca
     2101 gcgtctcacg cgccgggcgc gcaatctctg gccggatgcg cagggccgat cgacgggccg
     2161 cgcgctgttc gacggcgcac tcgctggcgg cgcgcaggcg ctcgggcagg cgatcgcggg
     2221 cattcgcgag ggcgcaagtg ccgatcttgt cagccttgat gcgaacgatc ccgcgcttgc
     2281 cggccgcatc ggcgattcca ttctcgatag ccggatcttc gccggcgcgg cgatcgattg
     2341 tgtgtggcgg catggagaga agcgtgtgag cggggggcgg catcatacgc gcgacgaaat
     2401 atccgcccgc tatcgccgta cgctggagcg gttgcttgcc tagaattcct ggagccgccg
     2461 gccgcgtata gcctttgcga gctgcaggtt caaatgccgg tgaacaggag atcgagcgcg
     2521 gagacgatcc gatagcgctc cgattccaga tttgcgacgg cgcgggccgc gagttgaaca
     2581 gggaaggcgc cgagttcctg cgccagaatc acatagggcc ggtcacgaac tctgacttcg
     2641 ggcatcagag gttcgaagtg gagaatcttc gcgggcgcaa caagcggcgc cacgatccgc
     2701 gtatcgagct ggtgcagctg atcggattgg aggacgacga aatacggccg cgatcggcgg
     2761 gtccttgagc ttgggttggg aaacacgtcg aactgccgca agttcaccac ttgcggtatt
     2821 cctctcccca caggccgttt tccgcgatga attcattata agattggaac gcctcgcggt
     2881 ggtcctcgtc gaaacggcga agctgttcct cccggagcag cccccgcagc gcgtcctcca
     2941 gcgtccggga tagattgatc ttcatcgcct ttgcttcgcg caaaacgtcc gcatcgatcg
     3001 acacgttggc ggatcgcctg cgcgccgcat gggcgccggt gcgttcagcc ctggtgcgcg
     3061 cgctgctgcg aaacggtttc ggcggatcgg agacgtgacc gggcatgcgc acactctatg
     3121 cgcatgatgc atgcgcggca agtgggacat gcttcacgcg cggaccatga caaagagggg
     3181 atcggagcgg tctcagcacg tccgcccacc tttccctgcg gatccgccgc cgggtggacg
     3241 ggcgcgccaa cgcccgtgct aacgtgacgc catgaggaac ctcgcgccgg ccgtcaagat
     3301 cgcctcgccg gccgcgctgc ggcttgagcg gagcgggcgc ggcgcgcggc tggtactggg
     3361 cggcgactgg atcgtcgccg aagccgcccg cgtcgacaag gagctgcgcg cgctgagctt
     3421 cggcggagcg aacgaactcg ccatcgacgg cgcagccatc gaactcctcg acagcgcggg
     3481 cgcctggctg atcctgcgca ccaagcgaga cctcgaagca aaacacgcca ccgtccaggg
     3541 tccggcgctt cccgagcgct atcgcgcgct gttcgacaag atggaacgcg agagcctcgc
     3601 gccgccggtc acccatcccc atccgctcaa cttcgaagaa ttcctcgcgc gcatcgggcg
     3661 cggcaccatg catgtgctgc gccagggtta cgacatggtg ggcttcctcg ggcggattgc
     3721 cgtcgagacc agcgaagcgg tcgtcgcgcc gcgccgcgag ctgccttttc ccgccttcat
     3781 tcagcagatc gaagagaccg ggctcaccgc gctgccgatc gtcggcatgc tcgccttcct
     3841 gctcggcgtc gtgctcgcct accagggcgc cgaccagctg aagcgcttcg gcgcgcagat
     3901 ctacaccatc gatttcctcg gcgtcggatt cctgcgcgag ctgggcggtc tcgtcaccgc
     3961 gatcatcatc gccgggcgct cgggttcggc gttcaccgcg cagatcggca ccatgcgcgt
     4021 caacgaagaa accgacgcga tgcagacgat cgggctcaac atcgccgaaa ttctcgttct
     4081 gccgcgcgtg ctcggcctgg tggtggcgtt gcctttgctc accttcttcg ccgacgtcgt
     4141 cggcatcgtc ggcggcatgg tgatgaccta tttcga
//
LOCUS       DASZMC010000228         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4488321,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000228 DASZMC010000000
VERSION     DASZMC010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4488321"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..646
                     /locus_tag="VG274_04545"
     CDS             137..646
                     /locus_tag="VG274_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWE05953.1"
                     /translation="MTALARPQAAAALEGAQSLGKHYLETLNLVERLHRQLLDVIKDE
                     LDRRDEREINSVQALLLFNVGDQELTAGELRTRGHYLGSNVSYNLKKLVEAGYIHHAR
                     SEADRRSVLVRLTPKGEVVREMLGELFDRHLSSLQPVGNVEERDLEGLNTSLRRLERF
                     WIDQVRFRL"
     gene            695..1336
                     /locus_tag="VG274_04550"
     CDS             695..1336
                     /locus_tag="VG274_04550"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HWE05954.1"
                     /translation="MPFARSHLTHHWRFYACFLFGATVYLVLGVLPPPVRLLAGGDAF
                     FLSYIAALGFLALHITPKKLDKKADVEDEGILLVILFALVMIGFCCFAIVTLLHQKHG
                     VDPFAIALAVAGAPLGWAMLHMMMAFHYAYLFYSEPGKKAGSGLIFPGTDEPGAADFL
                     YYAFVVGMTFQVSDVQVTSTRLRRMTLGHSIVSFFFNTCLIAMAVNAVVAIAA"
     gene            1444..2661
                     /gene="hemA"
                     /locus_tag="VG274_04555"
     CDS             1444..2661
                     /gene="hemA"
                     /locus_tag="VG274_04555"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HWE05955.1"
                     /translation="MTFAYLNRFRDALAGLRREGRYRVFTDIVRERGAYPNADFHTQG
                     AKRPVVVWCSNDYLGMGQHPKVLAAMHEAIDTTGAGTGGTRNISGTTHYHVELEAELA
                     DLHGKDGALLFTSGFVSNDATLATLAKILPGLIVFSDVFNHASMIEGMRNGGVEKRIF
                     GHNDMADLERLLAEADPDAPKLIAFESVYSMEGDFGKIEGICDLAEKYGALTYLDEVH
                     GVGLYGPRGAGVAARDNQMHRIDIIEGTLAKAFGVMGGYIAASNDLIDCIRSFAPGFI
                     FTTSLAPAIAAGVLASVRHLKESDVERETLQARAALLKKLFTEAGLPMMDSPSHIVPV
                     FVGDATLCKAVSDSLLYDHGIYVQPVNYPTVPRGAERLRFTPSPAHTEAMLHDLVHAL
                     DQVWTAHRLSRAA"
     gene            2658..2855
                     /locus_tag="VG274_04560"
     CDS             2658..2855
                     /locus_tag="VG274_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05956.1"
                     /translation="MNTPPKGGEVFIEIVIQGNVAKATAIDSVSGIEASIVGPANASR
                     AVLTDAARRKLEYLIRKKTQS"
     gene            complement(2865..3152)
                     /locus_tag="VG274_04565"
     CDS             complement(2865..3152)
                     /locus_tag="VG274_04565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWE05957.1"
                     /translation="MVGGLKTIVVKEGHEQEFEALFVALRAEIAAHEPGCVYYSLLRS
                     RTNPRAYIVEEQYQDEAAWEAHQSSTYGKTYFPKMRAIIDINVEYFDVVVS"
BASE COUNT          589 a          973 c          972 g          690 t
ORIGIN      
        1 caggccggaa tggatttctc cggtatcatc cagatgctac gcggggttat ctgataattt
       61 ttacgcccct ttaatcaatc gcccctattg tgatcgcata acaaggtggg ccctgcgaaa
      121 aagtggggaa aaagcgatga cagcattggc caggccgcag gcagctgcgg cgcttgaggg
      181 cgcacaatcg ctcggcaagc actatctcga aacactcaat cttgttgagc gtctgcatcg
      241 gcaattgctc gatgtgatca aggacgagct cgaccgccgc gacgagcgcg agatcaacag
      301 cgtccaggcg ctgcttctgt tcaacgtcgg cgatcaggag ctcaccgcgg gcgagttgcg
      361 cacacgtggg cattaccttg gttcgaacgt ctcctacaat ctcaagaagc tcgtcgaagc
      421 cggttatatc catcacgccc gttccgaggc cgaccgccgc tccgtgctgg tgcgtctgac
      481 gccgaagggc gaagtggtgc gcgagatgct gggcgaattg ttcgatcgcc atctgagttc
      541 gctacagcct gtaggcaatg tcgaggaacg tgatctcgaa ggcctgaaca cgagcttgcg
      601 ccgccttgag cgcttctgga tcgatcaggt ccgcttccgg ctgtagaagc ttcgcggctt
      661 tcgtgtcaca caggcgtctc cgccggaggc gccgttgccg tttgcacgat ctcatctgac
      721 gcaccattgg cgcttctacg cctgcttcct ctttggcgcg actgtgtatc ttgtgctggg
      781 agttttgccg ccacccgtgc gtctgctcgc gggcggcgat gcgtttttcc tgtcctatat
      841 cgcggcgctg gggttcctcg cgctgcacat cacgccaaaa aagctcgaca agaaggcgga
      901 tgtcgaggac gagggcatcc tcctcgtcat cctgttcgcg ctcgtgatga tcgggttctg
      961 ctgtttcgcg atcgtcacct tgctgcacca gaagcacggc gtagatccgt tcgcgatcgc
     1021 gctcgccgtc gccggtgcgc cgctcggctg ggcgatgctg catatgatga tggcgttcca
     1081 ctacgcctat ctcttctatt cggaacccgg aaagaaggcg ggcagcggat tgatctttcc
     1141 gggaaccgac gaaccgggcg ccgcggattt cctctattac gccttcgtgg tgggcatgac
     1201 gttccaggtc tccgatgtgc aggtgacctc gactcggctc cgccggatga ccctcgggca
     1261 ctccatcgtc tcgtttttct tcaacacctg cctgatcgcc atggcggtga acgcggtcgt
     1321 ggccatcgct gcctaggcga tatgcggcgt ttttagccaa gatatgcgac aggatgccta
     1381 ggatcggggc ccgctgctgg agtaatctcc cgcccggccg gactgccggc cgcgagggtt
     1441 atgatgacgt tcgcctacct gaacaggttc cgggacgctt tggccggtct gcgccgcgaa
     1501 gggcgctacc gtgtcttcac cgatatcgtg cgcgaacgtg gcgcctatcc caacgccgat
     1561 ttccacaccc agggcgccaa acggccggtg gtcgtgtggt gcagcaacga ctatctcggc
     1621 atgggccagc atcccaaggt tctggcggcg atgcatgagg caatcgacac gaccggcgcc
     1681 ggcaccggcg gcacccgcaa tatctccggc acgacgcact atcatgtcga gttggaggcc
     1741 gagcttgccg atcttcacgg caaggacggc gcgctgctgt tcacgtcggg cttcgtctcc
     1801 aacgatgcaa cactcgcgac tctggcgaaa attctccccg gcctgatcgt tttctccgac
     1861 gtgttcaacc acgcctcgat gatcgagggc atgcgcaatg gcggcgtcga gaagcgtatc
     1921 ttcgggcaca acgatatggc ggatctggag cggcttctcg ccgaagccga tccggatgcg
     1981 ccgaagctga tcgcattcga atccgtctat tcgatggaag gggatttcgg gaagatcgaa
     2041 gggatttgcg atctcgctga gaaatacggc gcgctcacct atctcgatga agtgcacggc
     2101 gtcggtcttt atggtccgcg cggcgccggt gtcgcggcgc gcgacaacca gatgcaccgc
     2161 atcgatatca tcgaaggcac gctggccaaa gcgttcggcg tcatgggcgg ctacatcgcc
     2221 gcgtcgaacg acctgatcga ttgcatccgc tctttcgcgc cgggcttcat cttcacgaca
     2281 tcgcttgcgc ccgccatcgc ggccggtgtg ctcgcgagcg tgcggcatct gaaggaaagc
     2341 gacgtcgaac gcgagacgtt gcaggcgcgc gcggcgttgc tgaaaaagct tttcaccgag
     2401 gccgggctgc cgatgatgga ttcgcccagc catatcgtgc cggttttcgt cggtgatgcg
     2461 acgttgtgca aggccgtctc ggattcgctg ctttacgatc acggcatcta tgtgcagccg
     2521 gtgaattatc ccaccgtgcc gcgcggcgcg gaacgcctgc gcttcacgcc gtcgcccgcg
     2581 cataccgaag cgatgctgca cgatctcgtc cacgcgctcg accaggtgtg gacagcacac
     2641 cgcctgagcc gcgccgcgtg aacacgcctc ccaaaggcgg cgaagtcttc atcgaaatcg
     2701 tgattcaggg aaacgttgcc aaggcgaccg cgatcgattc cgtgtccggc atcgaggcta
     2761 gtatcgtcgg tccggcgaac gcgtcgcgcg ctgtgctcac cgatgcagcg cggcggaagc
     2821 tggaatatct gatcagaaaa aaaactcagt cttagctggg aactctagga tacgaccaca
     2881 tcgaaatatt cgacattgat gtcgatgatg gcgcgcattt ttggaaagta tgtttttcca
     2941 taggtcgagg actgatgcgc ttcccacgcc gcttcatctt ggtactgctc ctcgacgata
     3001 taggctcgcg gattggtgcg tgatctgagt agagagtagt agacgcagcc cggctcatgc
     3061 gccgcaattt ccgcccgcag tgccacaaag agcgcttcga actcctgttc gtgcccttcc
     3121 ttcacaacaa tggttttcag gccgccgacc atttagcgcc tgctcttctt ccgcggcggg
     3181 gtggaggagc ggccgaggcc gatctttttg gcgaattcgg agcg
//
LOCUS       DASZMC010000229         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2572816,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000229 DASZMC010000000
VERSION     DASZMC010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572816"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..953
                     /locus_tag="VG274_04570"
     CDS             339..953
                     /locus_tag="VG274_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HWE05958.1"
                     /translation="MTEIDARAQRITSLSADFEQKKFTALLRRPLVSGGRVRVKGATM
                     RWDTQRPEPNVMLVTDKEFQLYYPAQKTVEVYPLDQKLAELAASPLPRLAVLHDRFSF
                     SRIPVAAMDAKADAEKFLALKLTPIPAELREHVQEVRVLLDVAAGNIVQAELTDADGD
                     RTVIRFSNAQVNVDVGELELKLPPGTKVTRPLEGLRGAPPGRSK"
     gene            977..>2011
                     /locus_tag="VG274_04575"
     CDS             977..>2011
                     /locus_tag="VG274_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWE05959.1"
                     /translation="MKSAASPTLPQEFVATTPRMALSAAKAAAPVVSQPVAPKAVGGI
                     GRVFLATRRWMNKASADFWLSFFFWHARRQPWFARGTKPFFLYFVWNYSAHLYGGTMA
                     NAARILGPSSTLVEREALARRIIGNFYDFVCDVGLCLGQSPEEMAQRIDRVEGHELYD
                     SIRHSGKTAGKGAIIATAHMGSFEVGAAALMQHEKRLHVLFRRDKLDLFEQIRSTLRK
                     KIGVVEVPLDQGWTIWMRLRDALANDEVVLIQGDRVMPGQKGQPVKFFDGHILMPTGP
                     ARLAMVSGSPIIPVFSVRTPKGKIRLFIEEPILVGDMPGGVSLDEAMNKLAAVLEKYV
                     RKYPDQWLENR"
BASE COUNT          382 a          595 c          645 g          389 t
ORIGIN      
        1 tttctcaaac aatacgtctc cgagccggat cgctcggcgc gcaagcgttc gctcgaggaa
       61 gtcgcactgc gaaggcaggc gcggcatctg tcgtgaggct ctgggtgttt cggcattggt
      121 atgattgacg ccggggaccc ggatcgggtg tccgacgcgg atccgggtcc tcgctttcag
      181 ctcgtccccg gcgtgaatcg tgctcgcggg atgtcacagt tatgttgcga ttcaagacct
      241 ttctcccggc attgctcctc gcgacgatcg cgtgcggcgc tcccccgacg acgcaacctg
      301 catccgcgcc atccgtcgat cctgccctct gggcgaagat gacggagatc gacgcccgcg
      361 cccagcgcat tacttccctc tccgctgatt tcgagcaaaa aaaattcacc gcgctgttgc
      421 gccggccgct ggtttccggc ggccgggtgc gggtgaaagg cgcgactatg cgctgggaca
      481 cgcagcggcc cgagcccaac gtcatgctcg tgacggacaa ggaatttcag ctctattatc
      541 cagcacagaa aaccgtggaa gtgtatccgc tcgaccagaa gctcgccgag cttgcggcgt
      601 cgcccttgcc gcggctggcg gtgcttcacg accgtttttc attttcgcgg attcctgtag
      661 cggcgatgga cgccaaggct gatgcggaaa aattcctggc gctgaagctg acgccgatcc
      721 cggcggagct gcgcgagcat gtgcaggaag tgcgggtatt gctggacgtg gcggcgggca
      781 atatagttca agccgagttg accgacgccg acggtgatcg caccgtaatt cggttcagca
      841 atgctcaggt caacgtggat gtgggtgaac ttgaattgaa attgccgccg ggcacgaagg
      901 tgacccgtcc gcttgaaggg ctgcgcggag cgccacccgg gcgatcgaag tgagaccgtc
      961 agccgtaata ccaaacgtga agagcgccgc cagtcccaca ttgccgcaag agtttgttgc
     1021 gacgacgccg cgcatggcgc tgtcggccgc gaaggcggcg gcgccggttg tcagtcaacc
     1081 tgtcgcgccg aaagccgtcg gtgggatcgg ccgagtcttc ctcgcaaccc ggcggtggat
     1141 gaacaaggca tcggcggatt tctggctcag tttcttcttc tggcacgccc gacggcaacc
     1201 ctggtttgcc cgtggaacca agccgttctt cctctacttc gtctggaatt attccgcgca
     1261 cctgtacggc gggacgatgg cgaacgccgc gcgaatcctc ggcccgtctt ccacgctcgt
     1321 ggagcgcgaa gcacttgccc ggcggatcat cggcaacttt tacgatttcg tctgcgacgt
     1381 ggggctgtgc ctcggacaat cgccagaaga gatggcgcag cgcatcgatc gcgtcgaggg
     1441 gcatgagctt tacgattcca ttcgtcacag cggaaaaacc gccggcaagg gcgccattat
     1501 cgcgacggcg catatggggt cattcgaggt cggagcggcg gcgctgatgc agcacgagaa
     1561 gcgcctgcac gttctcttcc gccgcgacaa gctcgatttg ttcgagcaga tccgctcgac
     1621 cttgcgaaag aagatcggcg tggttgaagt gccgctggac cagggatgga cgatctggat
     1681 gcggctgcgc gatgcgctgg cgaatgatga agtggtgctg atccagggag accgggtgat
     1741 gcccgggcaa aagggccagc ccgtaaaatt cttcgacgga catatcctca tgccaacggg
     1801 gccggcgcgc ctggcgatgg tcagcgggtc cccgatcatc ccggtattct cggtgcgaac
     1861 gcccaaaggc aaaattcggc tattcatcga agagccgatc ctcgtgggcg acatgcccgg
     1921 cggggtctcg ctggatgaag caatgaacaa actggcggcg gtgctggaaa agtacgtccg
     1981 caaatatcct gatcaatggc tggaaaaccg t
//
LOCUS       DASZMC010000230         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1749714,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000230 DASZMC010000000
VERSION     DASZMC010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749714"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..606)
                     /locus_tag="VG274_04580"
     CDS             complement(1..606)
                     /locus_tag="VG274_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HWE05960.1"
                     /translation="MLLVVDLLGTYVFAVEGAMTAIRANLDLLGLLVLSFATALGGGI
                     IRDMLIGAVPPNSIRDWRYGATAFAGGGTVFFFYQFFQSVPQPLMITLDAAGLALFAV
                     AGAEKALEFGINPLIAVLMGGVTGVGGGTVRDVLLTQVPGVLRTDIYAAAALAGAAVV
                     VVGIRMKMPRTLAMSLGGIACFLLRMVAVWQHWNLPKVMVH"
     gene            complement(720..1727)
                     /locus_tag="VG274_04585"
     CDS             complement(720..1727)
                     /locus_tag="VG274_04585"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWE05961.1"
                     /translation="MSEIHGLAVHAAGAHLLPYKYDPGELQANEVEIKISHCGVCHSD
                     IHLIDNDWGFSKYPFIPGHEIVGTVVAVGSSVADRKLGDRVGVGWQADSCGVCEWCRQ
                     GDEHLCAKSQPTCVGRNGGYADRIRVNSHFAIPVPAVLESENVAPLLCAGITVYSPLR
                     NHGVRPSSRVGVIGIGGLGHLGIQFAKAFGAEVTAFSTSKDKEAEARSLGAHHFLNTQ
                     DTGALKKVAGSFDFLLSTVSADQDWQGYVNALRPKGMLCVVGASPSPIQINAFSLIAA
                     QRAISGSPSGSPRDLHEMLDVAARHGVKAITERFAMSKANDAVAKVKKNQVRYRAVLA
                     N"
     gene            complement(1871..>2598)
                     /gene="hemC"
                     /locus_tag="VG274_04590"
     CDS             complement(1871..>2598)
                     /gene="hemC"
                     /locus_tag="VG274_04590"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWE05962.1"
                     /translation="RIDLAVHSLKDLPTQLAPQFTLAAIPKRADARDVWVCEPYWSLN
                     TLPMGGRIGTTSPRRRAQLLALRPDVQFVDIRGNIDTRLKKLAAGQCDALVLAAAGLD
                     RLKRAESVHHRFSPEELCPAPGQGALAIETRSANHIIDEDRDAYIRQAVAFFNHPETR
                     FCVEAERVALDALGGGCSIPIGVHCVPEYEKWRMFAQVLAPDGEAMIQIDTEATLDTT
                     PECLGHWVADDLKSKGALSLLTS"
BASE COUNT          524 a          811 c          781 g          482 t
ORIGIN      
        1 tcaatgcacc atcaccttcg gcaggttcca atgctgccac acggcgacca tgcgcagtag
       61 gaaacaggct atcccgccaa gactcatcgc cagggtacga ggcatcttca tccttattcc
      121 cacaactaca accgctgcac ccgccagcgc ggccgccgca tagatatccg tccttaatac
      181 gcccggaacc tgcgtcaaca gcacatcgcg caccgtgccg ccgcccactc cggtcacgcc
      241 tcccatcagc accgcaatca gcggattgat gccgaactcc agcgccttct ccgctccggc
      301 gacagcgaac aacgccagcc cggcagcatc gagggtaatc atcaaaggct gcgggacgct
      361 ttgaaaaaac tggtagaaaa agaagaccgt gccgccgccg gcaaacgccg tcgcgccgta
      421 gcgccagtcg cgaatgctgt tgggcggtac agcgccgatc agcatgtctc gaatgattcc
      481 tccgcccagc gcggtggcga acgacaggac cagaaggccc agaaggtcga ggtttgcgcg
      541 gatcgcggtc atggctcctt ccaccgcgaa cacgtaggtg cccagaagat cgacgacgag
      601 cagcaaaacg tcaggattgt atcgggcaaa tctgcgtctc tctgccgcca tcgacccccc
      661 tccgaaaaga aaggcgaaag ccgaagctcc cgcctttttc ttatctcacc atggccacgt
      721 tagttggcca gaaccgccct gtatcgcacc tgatttttct tgaccttggc cacagcatcg
      781 tttgccttgc tcatggcaaa gcgctcggtg atggccttga cgccatgccg cgctgcaaca
      841 tcgagcatct cgtgcagatc gcgcgggctg ccggaggggc tgcccgaaat cgctctctgc
      901 gctgcaatca aagagaacgc attgatctgg ataggagagg gggaagcgcc taccacgcag
      961 agcattccct tggggcgcag agcattcaca tatccctgcc agtcctggtc ggcgcttacg
     1021 gtcgagagca gaaaatcgaa cgatcccgcc accttcttta atgctccggt gtcctgggtg
     1081 ttcaaaaaat gatgcgcgcc cagcgagcgg gcttcggcct ctttgtcctt cgaggtcgaa
     1141 aacgcggtca cttctgcgcc gaacgccttg gcgaactgga ttcccagatg cccgagaccg
     1201 ccgatgccga tcacgcccac acgcgatgaa ggacgaacgc catgattgcg caggggacta
     1261 tagaccgtaa ttccggcgca cagcagaggg gccacgtttt cgctttcgag caccgcgggc
     1321 actggaatgg caaagtgaga attgacgcga atcctgtccg catagccgcc gtttcgcccg
     1381 acgcaggtcg gctgcgactt ggcgcagaga tgctcgtccc cctgacggca ccactcgcag
     1441 acgccgcaac tatccgcctg ccagccaacg ccgacgcggt cgccaagctt gcggtctgcc
     1501 acgctgctgc ccacggctac aaccgttccg acgatctcgt ggccggggat gaagggatat
     1561 ttgctgaatc cccaatcgtt gtcgatcagg tgaatgtcgc tgtggcacac cccacagtgc
     1621 gagatcttga tctcgacctc attcgcctgc aactcgccgg gatcgtactt atagggcagc
     1681 aaatgcgcac cggctgcgtg cacagccaaa ccatgtatct cactcatttg aagtctttcc
     1741 tttagacgcc tgatgtactc gtccgcgcct gctgtctgat gctacaacat cgccatcgac
     1801 aaatatttta gccttgcagt ggttcggcat cgagcctcca gaatcgaagc ggcaagttgg
     1861 ccctggaaca ttaggaagtc agcaagctga gggcgccctt cgacttgagg tcgtcagcga
     1921 cccaatgacc caggcactcg ggagtcgtgt cgagggtggc ttcggtatcg atctggatca
     1981 tggcttctcc atccggggcg agcacctggg cgaacatgcg ccacttttcg tactccggaa
     2041 cgcagtgaac gccgatcgga atggagcagc ctccgccgag tgcgtcgagc gcgacccgtt
     2101 cggcttcaac gcagaagcgc gtctcagggt ggttgaagaa ggcgacagcc tgacgaatgt
     2161 aggcgtcgcg gtcctcgtcg atgatgtggt tggcgctgcg ggtttcgatg gccagcgcgc
     2221 cttgcccggg agcgggacaa agctcttccg gcgagaagcg gtgatggacg gactcggctc
     2281 gtttgagacg gtcgagtccg gcggcggcga gaaccagagc gtcgcactgg ccggcggcca
     2341 gcttcttgag gcgggtgtcg atgttaccgc ggatgtcgac gaactgcacg tcagggcgga
     2401 gcgccagaag ctgggcgcgt cggcgcgggc tggtcgtgcc gattcgtccg cccatcggca
     2461 gcgtgttgag cgaccagtac ggctcgcaga cccagacgtc acgggcgtcg gcgcgtttgg
     2521 gaatggcggc gagggtgaac tgcggcgcga gctgcgtggg caggtctttg aggctgtgga
     2581 cggcgaggtc gatgcggc
//
LOCUS       DASZMC010000231         5954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3946435,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000231 DASZMC010000000
VERSION     DASZMC010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946435"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2219
                     /gene="pheT"
                     /locus_tag="VG274_04595"
     CDS             <1..2219
                     /gene="pheT"
                     /locus_tag="VG274_04595"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWE05963.1"
                     /translation="ITMKTAECEGVENVGALLSGAVPARVESAEPVPGSNHLSKAVVD
                     AGHYGRRTVVCGAPNCRPGIETVYVPLGRKTIAGVVSDGMLASAAELEINKDHGGIVE
                     LEGSAELPASDSVIEIDNKSITHRPDLWGHYGMAREVAAITGKTLRDCVNMSLLPAPG
                     GPINVQIEDVTLCRRYSALVFENVTVKPSPLWLQFRLTSIGLNPINNIVDLTNYLMAE
                     LAQPMHAFDREKLAGDTIWARPARAGERILALNKEQYELAPSNLVIADASGPIAIAGV
                     IGGNDSAISETTTTIVLESANFNASSVRKTSAALKLRTDASMRFEKAQDPHNTVRALA
                     RAIELLPLISPGIRLAGGLADRSVELKPPPPVPLNLDWLARKLGRALDLTEVRRILES
                     LEFGCTENGPNALTVSIPSWRGTKDVALPDDLVEEVGRMIGYDTIPPVPPLVPCKPSY
                     DPPEREFLRNVRRALADQGFTEVSNYSFISEEEARRFGFALEDHIRVLNPIAAGQELL
                     RTSLLPGIYRNILENAKHFDEFRLFEIGNEIHNDAVHRNAPHAMAALCTKHGDGRAAL
                     LELKRTAECLARGLHVRPAQPHVWEHPTRCAEIWWNGREIGRLSELLPTLVESARAAV
                     LDLDLTLLRELAPDRAKYNAVHRFPTSAFDLSILAGARELAGDLELEIRKFAGDLLEG
                     VEYVREYEGPPLPEGKKSVSFRLVVGGTKTLSSDEIAAIRASIIAGLQGVGYELRV"
     gene            2226..3530
                     /locus_tag="VG274_04600"
     CDS             2226..3530
                     /locus_tag="VG274_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWE05964.1"
                     /translation="MTQRSPTFRLLTGLVITLLAVGIYSGYTIRQLRSLRTLQTETID
                     RNRTDTLLLLRIQNGLNSLALTMRDMLDEQEPYPLTAWAPQFRRIRSDLEDAFGREEK
                     FSLADRSPNQRRYLADSFRQFWDGLDRIFDQARAGDEREARTRIRLSLQARQAALSTA
                     VSRLLIQNNESEQTAAARTQEIYAGVERNVYIFLSAMLVVILLTSLYLVHHIRRLFAQ
                     VDALSTRRGELAQQLISMQESTFRSISRDLHDDFGQILTAIGVMLQRADRRPGDLLEV
                     RQIVQDTLEKVRALSHALHPVALDEVGLESALDSYLSGFERQTGIAITYEKHGEAREL
                     DRETAVHLYRVTQEALNNVAKHARVKQALVRLTFTPAEAVLEIQDFGVGITRNPDRGL
                     GLVSMRERTEMMKGKLEIARGETGGTLVRVVVPVSAMEGVHA"
     gene            3523..4146
                     /locus_tag="VG274_04605"
     CDS             3523..4146
                     /locus_tag="VG274_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWE05965.1"
                     /translation="MPEPRIKVFLVDDHALVRRGFRRMLEDDPEIEVVGEASDGHEAV
                     EAVRALKPRVVVMDFALPSMNGAVATRRIGEACPDTAVLMLSMHEETTYVRTCLEAGA
                     RGYLLKNAMDLELVSAVKKIAAGEQVLDPRIGRLAQEPEPALTTRELEVLQLIVHGKS
                     NKDIAVVLGLSANTIAVHRANIMQTLGIHNTAELVVHAIRRGLVSIP"
     gene            4143..5795
                     /locus_tag="VG274_04610"
     CDS             4143..5795
                     /locus_tag="VG274_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HWE05966.1"
                     /translation="MNRRTFLFGSAPLLMAAGSGDALRLSDVTSSAGIAFHHNSGAFG
                     AKYLPETLGPGCAFLDYYRDGWLDILLVNGCDWPGHTRTRSTLALYRNNRNGTFTDVT
                     ARAGLAVEMYGMGVAIADYNNDGFPDILITAVGQNRLFRNTGKGAFVDVTEKSGLGGR
                     NGFSTSALWFDYDRDGLLDLFVCNYVQWSPEHDVFCSVDGKNKSYCTPEAYHGSTCWL
                     FRNRGNGTFEDVTAKSGVFDTTSKSLGVALLDHDRDGWLDLFVANDTQPNKLYRNNRN
                     GTFEDIAVHAGVAFSEDGKARAGMGVDVADFDNSGAAGIAITNFDEEMMAIYRPARGG
                     SYADAALKSGVGQASRLRLGFGCAFLDIDLDGHLDLAAVNGHIDETVRNISGNHGYAQ
                     PPHLFLNDGRGNFHDIAAQTGGAFAAPKVGRGLAYGDFDRDGDLDLLVTTNHGPAYLY
                     RNDLANGNRSLRLELQGVKSNRDAIGAVVRVTTPDGAQLRMVKTGSSYLSQSELTLTF
                     GLGRRDRADRITIEWPSGATQEFRAIAAGSWRCVEGQNLTAA"
BASE COUNT         1172 a         2086 c         1620 g         1076 t
ORIGIN      
        1 tcatcaccat gaaaaccgcc gagtgcgaag gcgtcgaaaa cgtcggcgcg ctgctctccg
       61 gagccgtgcc cgcccgcgtt gaatccgccg agcccgtgcc cggcagtaat catctttcga
      121 aagcggtagt cgacgccggc cactacggcc gcagaaccgt ggtctgcggc gcgccgaatt
      181 gccgccccgg aatcgaaacg gtatacgtcc cccttggccg gaagacaatt gcgggcgtgg
      241 tcagcgacgg catgctcgcg agcgcggccg agctcgaaat caacaaagat cacggcggaa
      301 tcgtggaact ggaaggctcc gccgaactgc cggcgtccga cagcgtcatc gaaatcgaca
      361 acaaaagcat cacgcatcgt cccgatctgt ggggccacta tggcatggcc cgcgaagtcg
      421 ccgccatcac cggcaagact ctgcgcgatt gcgtcaatat gtcgctgctg ccggctccgg
      481 gcggtcccat caacgtccag atcgaagatg tgacgctttg ccggcgttat tccgcgctcg
      541 tcttcgagaa cgtaaccgta aaaccctcgc cgctctggct gcagttccgc ctcacttcaa
      601 tcggcctgaa cccgatcaac aacatcgtcg atctcacgaa ctacctgatg gccgaactgg
      661 ctcagccgat gcacgcgttc gaccgtgaaa agctcgccgg ggacaccatc tgggcccgcc
      721 ccgcccgcgc cggagaacgc atcctcgcgc tcaacaaaga gcaatacgag ctcgcgccct
      781 cgaacctggt tatcgccgac gcctccggtc cgatcgcgat cgccggcgtt atcggcggta
      841 acgacagcgc aatttcggaa accaccacca ccatcgttct cgagagcgcc aatttcaatg
      901 cttcgagcgt gcgcaaaaca tccgccgctc tcaagctgcg caccgacgcc tccatgcgct
      961 tcgaaaaagc gcaggacccg cacaacaccg tgcgcgccct ggcccgcgcc atcgaacttc
     1021 tgcccctgat ctcgcccggc atccggctcg ccggaggcct cgccgatcgg agcgtcgaac
     1081 tgaaacctcc gccgccggtt ccgctgaacc tcgattggct tgcccgcaaa ctgggtcgcg
     1141 cgctcgatct gacggaagtt cgccgcatcc tcgaaagcct tgaattcggt tgcacggaaa
     1201 acggcccgaa cgctctgacc gtaagcatcc cgagctggcg cggcacgaaa gacgtcgccc
     1261 tgcccgacga tctcgtggaa gaagtcggcc gcatgatcgg ctacgacacg attccgcccg
     1321 tcccgccgct cgttccctgc aagccgtctt acgatccgcc cgagcgcgag ttcctccgca
     1381 acgtgcgccg cgcccttgcg gaccagggct tcaccgaagt ctccaactat tccttcatca
     1441 gcgaagaaga ggcccgacgc ttcgggttcg ccctcgaaga ccacattcgc gttctgaatc
     1501 ccattgccgc cggacaggag ttgctccgaa cctcacttct ccccggcatc tatcgcaaca
     1561 tcctcgaaaa cgccaaacat ttcgacgagt ttcgcctctt cgagatcggc aacgagatcc
     1621 acaacgacgc ggttcaccgg aacgcgccgc acgccatggc cgcgctctgc accaaacacg
     1681 gcgatggccg cgccgcgctc ctcgaactga agcgaaccgc cgaatgcctc gcccgcggcc
     1741 tgcatgttcg tcccgcgcaa ccgcatgtct gggaacaccc cacccgctgc gccgagatat
     1801 ggtggaacgg ccgcgaaatc ggccgtctca gtgaacttct tcccacgctt gtcgaatcgg
     1861 cccgcgccgc cgtgctcgat ctcgatctga cgctgctacg cgaactcgcg cccgatcgcg
     1921 ccaaatacaa cgccgtccac cgcttcccca ccagcgcttt cgatctttcc atcctcgccg
     1981 gcgcccgtga gttggccggt gacctcgaac tcgaaatccg caagttcgcc ggtgacctgc
     2041 tcgaaggcgt tgaatacgtc cgtgaatacg aagggccgcc gctgccggaa ggaaagaaga
     2101 gcgtctcctt ccggctggtg gtgggcggca cgaaaactct atcttccgac gagatcgccg
     2161 ccattcgcgc ctccatcatc gccgggcttc agggcgtagg ctacgagttg cgggtttaaa
     2221 ctgttgtgac gcagcgatct cccacattcc gcctgctcac gggactcgtc atcactctcc
     2281 tcgctgtcgg catctactcc ggctacacga tccgccagct ccgtagtttg cgaacgctgc
     2341 agaccgaaac catcgaccgc aaccgcaccg acaccctact cctcctccgc attcagaacg
     2401 gcctcaattc cctcgcgctc accatgcgcg acatgctgga tgaacaagag ccctatcccc
     2461 tcaccgcctg ggccccgcag ttccggcgca tccgttccga cctcgaagac gctttcgggc
     2521 gtgaagaaaa attttctctg gcggaccgtt ctccgaacca gcgtcgatat ctcgccgatt
     2581 ctttccggca gttctgggac ggtctcgatc gcatcttcga tcaggcgcgc gccggcgacg
     2641 aacgcgaagc ccgcacacgc atccgcctct cccttcaggc tcgccaggcc gcgctcagca
     2701 ccgccgtttc ccgcctgcta atccagaaca acgagagcga acaaaccgcc gccgcccgaa
     2761 cgcaggagat ctacgccggg gtcgagcgca acgtctacat ctttctttcg gcgatgttgg
     2821 tcgtaattct gctgaccagt ctttatctcg tccatcacat acgccgtctc ttcgctcagg
     2881 tcgacgctct ctccacacgc cgcggagaat tggcccagca gttgatttcc atgcaggaga
     2941 gcaccttccg ctccatctcg cgcgatctgc acgatgactt cggccagatc ttaaccgcca
     3001 tcggcgtcat gctgcagcgc gccgaccgcc gccccggcga cctgctcgaa gtacgccaga
     3061 tcgtgcagga cacgctcgaa aaagttcgcg ctctctcgca cgcgctgcat ccggtcgcgc
     3121 tggatgaagt cggcctcgag agcgccctcg attcttatct gtccggcttc gaacgtcaga
     3181 ccggcattgc gataacgtac gagaaacacg gcgaagcccg cgagctcgat cgcgaaaccg
     3241 ccgtgcatct ttaccgcgtg acacaggaag cgctcaataa cgtcgccaaa cacgcccgcg
     3301 tgaagcaggc cttggtgcgc ctcacgttca cgcccgccga agccgtgctg gagatccagg
     3361 attttggcgt cggcatcacg aggaatcccg accgcgggct gggcctcgtg tccatgcgag
     3421 aacgtaccga aatgatgaaa ggcaaactgg aaatcgcacg cggagaaacc ggcggaacgc
     3481 tcgtgcgcgt cgtcgttccc gtctccgcca tggagggtgt acatgcctga gccgcgcatc
     3541 aaagtgtttc tggtcgacga ccacgcgctc gtgcgccgcg gatttcgccg catgctcgaa
     3601 gacgatcccg agatcgaagt ggtcggcgag gcgagcgatg gccatgaagc ggtggaggcc
     3661 gtgcgcgctc tcaaaccccg cgtcgtcgta atggatttcg cgctgccaag catgaatggc
     3721 gccgtcgcca caaggcgcat tggcgaggcc tgccccgata ccgctgtcct catgctcagc
     3781 atgcatgagg agaccaccta cgtgcgcacc tgcctggaag cgggagcccg cggctacctc
     3841 ctgaaaaatg ccatggacct cgagctcgtt tcggccgtca aaaaaatcgc ggcaggcgag
     3901 caggtcctcg acccgcgcat cggccgtctc gctcaggagc ccgagcctgc gcttaccacc
     3961 cgcgagctcg aagtcctgca gttgatcgtc cacgggaaat ccaacaagga catcgcggtc
     4021 gtacttgggc tcagcgccaa caccatcgct gtccaccgcg ccaacatcat gcagacgctc
     4081 ggaattcaca atacggcgga actcgttgtc cacgccattc gacgagggct ggtcagcatc
     4141 ccgtgaaccg ccggactttc ctgttcggaa gcgcgccgct cctgatggcg gccggcagcg
     4201 gcgacgcctt acgcctgtcc gatgtcacct cttcggcggg aatcgccttc catcacaaca
     4261 gcggcgcctt cggcgccaaa tacctgcccg aaacgttagg gcccggctgc gcctttctcg
     4321 attactatcg cgatggctgg ctcgacatcc tcctcgtcaa cggttgcgac tggcccggcc
     4381 acacacgcac acgctccaca ctcgcgctct accgcaacaa tcgcaacggc accttcaccg
     4441 atgtcacggc gcgagccggc ctcgcggtcg aaatgtacgg catgggcgtc gcgatcgccg
     4501 attacaacaa cgacggtttt cccgatatcc tcatcacggc cgtcggccag aatcgcctct
     4561 tccggaacac tggcaaaggc gcgttcgtcg acgtcaccga aaaatccgga ctcggcggac
     4621 gcaacggttt cagcacctcc gcgctctggt tcgattacga tcgcgacggc ctgctcgatc
     4681 tcttcgtgtg caattacgtg cagtggtcgc ccgagcacga tgtcttctgc agcgtcgacg
     4741 gaaaaaataa atcctactgc acccccgagg cctatcacgg ctccacctgt tggctcttcc
     4801 gcaatcgtgg caacggcacg tttgaggatg tcaccgcgaa aagcggcgtc ttcgacacca
     4861 cttcgaaatc ccttggcgtc gctctgctcg atcacgaccg cgatggctgg ctcgatctgt
     4921 tcgtcgccaa cgacacgcag cccaacaagc tctatcgcaa caatcgcaac ggaaccttcg
     4981 aagatatcgc cgtgcacgcg ggcgttgcgt tcagcgaaga cggcaaggcc cgcgccggca
     5041 tgggcgtcga tgtggccgat ttcgataact ccggcgcagc cggcatcgcg atcacgaact
     5101 tcgatgagga aatgatggcg atctatcgcc cggcgcgcgg cggatcgtat gccgacgccg
     5161 ctttaaaatc aggcgtcggt caggcatccc gcctgcgcct cggcttcggc tgcgcttttc
     5221 tcgatatcga tctcgacggc catctggatc tcgccgccgt caacggccat atcgacgaaa
     5281 ctgtgcgcaa catcagtggc aatcacggct acgcccagcc gccgcatctc tttctgaatg
     5341 acggccgcgg caatttccat gacatcgcgg cccaaacagg cggcgctttc gcggcgccca
     5401 aagttggacg cggcctcgca tacggagatt tcgatcgtga cggagatctc gatctcctcg
     5461 tcaccaccaa ccacggcccc gcctatctct atcgcaacga tctcgccaac ggcaatcgtt
     5521 cgctgcgtct tgaactgcaa ggcgtgaaat cgaatcgcga cgccatcggc gcggtcgtgc
     5581 gcgtcacaac tcccgatggc gcgcagttgc gcatggtgaa aacaggttca agctatcttt
     5641 cgcaatccga gctcaccctc accttcgggc tcggacgccg cgacagagcc gaccggataa
     5701 ccatcgaatg gccatccgga gcgactcagg aattccgcgc catcgcggcg ggttcatggc
     5761 gctgcgtcga aggtcagaac ctcactgccg cctaacatcc cgcagcccga taggcggaaa
     5821 atacgcgccc ttcaactctc gcttccacca attcccggtt cgccgcagtt catcgatgct
     5881 cgtgaaccgg tagtcataaa ccatcgcgcg gatctgcttc ggcggcgcgc cgccgaacgg
     5941 gtcgcgttcg agca
//
LOCUS       DASZMC010000232         5155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_657447,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000232 DASZMC010000000
VERSION     DASZMC010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657447"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VG274_04615"
     CDS             <1..1147
                     /locus_tag="VG274_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWE05967.1"
                     /translation="NNALRIRVHRLAQAAPVNQDPDRTISDTLAQALAPEVDATLVGI
                     FKELDMALIKIPAHDLPALKFADYRKLRQGQVVFAFGSRQGLGNSMSMGVVSSVARQP
                     DQDSPFVYIQTDAPINPGDSGGPLVNTAGEIVGMDTFILSQSGGSEGIGFAIPSTLIE
                     LSADQLKKYGHMHRQLIGIGVQTITPPLADALKLPRDSGVLVSDVLPKGPAEEAGVKL
                     NDIVTAVDGRRIENVPMFTTALLSHPAGEHVKLEVMRGKETIPLDIEAVEESHKSDRL
                     SDLIDPDKNRISQLGIVGIAIVPELAEMLQGLRGQYGVIVAALSASSSASMTGLQVGD
                     VIHEVNGTAVESVDQLRSTMQKFKRNDSVALFIERDGKLQYLAFEVE"
     gene            1183..2307
                     /locus_tag="VG274_04620"
     CDS             1183..2307
                     /locus_tag="VG274_04620"
                     /EC_number="4.2.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF043002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901605 - alpha-amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cis-3-hydroxy-L-proline dehydratase"
                     /protein_id="HWE05968.1"
                     /translation="MKITGIAAYRVELPLHEGSYKWSGGNSVDVFDSTVVEISTDAGI
                     TGYGEICPLGPAYLPAYAAGARVGIAEIAPQLLGLDPTELQVMNRRMDQTLRGHPYVK
                     SAIDMACWDILGKASNQSVATLLGGYYEKDFPLYRAISQDTPKAMAKSVGHYRSEGYT
                     KFQLKVGGNPDLDMERIHAAAAQLKAGDVLIADANTGWTQHQALRVADAVRGVDVYIE
                     QPCYSYEECLAVRRHTDRPFVLDEVIDGVQMVLRGHAEQAMDVINLKISKVGGLTKAR
                     QIRDLCVSLGIAMTIEDTWGGDIVTAAIAHMAHSTPPELLFTATDFNSYVTVSIAEGA
                     PQRREGRMQASREPGLGVRPRMEVLGKPVLHFAADSTSMK"
     gene            complement(2274..2840)
                     /locus_tag="VG274_04625"
     CDS             complement(2274..2840)
                     /locus_tag="VG274_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWE05969.1"
                     /translation="MTFALDYKRDLLEAIERIDTEAVHRAIEWFRETRDSGRAVFVCG
                     NGGSASTASHFVCDMVKGASMNRASRFRVMALCDSLPTLTAYSNDVSYDVVFAEPLRN
                     FAQPGDLFMAISGSGNSPNVLRAMQVADEVGCRTIALTGRDGGKLGPMAQLNIQVGIP
                     HMGRIEDAHMIVCHMICYYFMDVESAAK"
     gene            complement(2837..3685)
                     /locus_tag="VG274_04630"
     CDS             complement(2837..3685)
                     /locus_tag="VG274_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWE05970.1"
                     /translation="MHVLAIDIGGTKFSVAAFDGDQMVRRVSQPTDRAGSRDWMLARI
                     AEIHSEWKRDFAFERCGVGFGGPVNFASQRVMLSTHVGGWRDFDLPAALREILGVPVI
                     MDNDANVGALGEAVHGAGRGCSPLFYMTLSTGIGGGIYDNGQIWRGADSYGGEIGHLT
                     IRPDGPECLCGSFGCFERMCCGLWLERDHGKPAKDLMQDPEFVRRYVVDLALGLKACI
                     MLLNPARIVIGGGISKAGGLLFDPLRAELRRQITSWSAARIDVVPAQLADDSVLYGAL
                     ALVNQL"
     gene            complement(3667..4653)
                     /locus_tag="VG274_04635"
     CDS             complement(3667..4653)
                     /locus_tag="VG274_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWE05971.1"
                     /translation="MTPHEILAAIPKLSALVVGDVCLDRWCTYDPAVSEPSRETGIPR
                     LGVVATEVTAGAAGTVANNLAALGCNRVSVLGVIGEDGFGFELSRALDSRGIESRFCV
                     RTSAMQTFTYTKLINRQAGVEDQPRVDFINIVPLDSSVEREVIAKLHQAIADFDVVII
                     SDQAETGQGGVVTPAVRDALCELARLHPHKTFIVDSRARITEFRNVIAKPNQHEGEAA
                     CGALFGRVDYAALRAHLSTDLLMITHGGDGVLLVHPDREEWVRTQPVADPVDICGAGD
                     SFSAGSALALAAGASAAEAASFGNLAASIAIMQKGTGVASREEIAQRCTSSQ"
     gene            complement(4650..>5155)
                     /locus_tag="VG274_04640"
     CDS             complement(4650..>5155)
                     /locus_tag="VG274_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05972.1"
                     /translation="SSGFPNIELSVLAAAWRLNIPVTVHAGIGYDIIHEHPNCDGAAV
                     GDASYRDFLIFASAVERLESGVLLCFGSAVMGPEVYLKALSMARNVAHQQGREIRHFA
                     TAVFDLMPLAGDIHKERSKTEAAYYFRPYKTILVRTVADGGESFYFCGDHRATLPALR
                     RAALDRL"
BASE COUNT         1024 a         1382 c         1857 g          892 t
ORIGIN      
        1 gaacaacgcg ctgcgaattc gtgtgcatcg gctggcgcag gccgcgccgg tgaaccagga
       61 tcccgaccgc accatctctg acacgctcgc gcaagcattg gcgccggagg tggatgcgac
      121 gctggtcggc atattcaaag agctcgacat ggcgctgatc aagatcccgg cccacgattt
      181 gcccgcactg aagttcgcag actataggaa gctgcggcag ggacaagtgg tgttcgcctt
      241 cggaagccgg cagggcttgg gaaactcgat gagcatgggc gtggtgagtt ctgtcgcgcg
      301 gcaaccggac caggacagcc ccttcgtcta catacagact gatgcgccga tcaatccggg
      361 cgatagcggc ggcccgctgg tgaacacggc gggagagatc gtcggcatgg acacgtttat
      421 cctgagccaa tcgggcggca gcgagggcat cggattcgct attccgagca cgctgatcga
      481 gctttcggcg gatcagttga agaagtacgg ccacatgcac cgacagttga tcggcattgg
      541 cgtacagacg attaccccgc cgctggccga cgcgctgaaa ctgccgcgcg actcgggcgt
      601 gctggtgtct gacgtcttac cgaagggacc tgccgaggag gcgggcgtaa agttgaacga
      661 catcgtgacc gcggtggacg ggcggcgcat tgagaatgtg ccgatgttta cgactgcgtt
      721 gctgtcacat cccgcgggcg agcacgtaaa gctggaagtg atgcgcggaa aggagacgat
      781 tccgctggat atcgaggcgg tcgaggagtc gcacaagtcg gaccggttga gcgatttgat
      841 cgacccggat aagaaccgca tctcgcaact tggaatcgtc ggtatcgcga ttgtgccgga
      901 gcttgcggag atgctgcaag gactgcgtgg gcagtatggc gtgattgtgg ccgcgctttc
      961 ggcgtcgtct tcggcgagca tgacgggctt gcaggtgggc gacgtgatcc atgaagtgaa
     1021 cggcacggcg gtcgagagcg tagatcaact gcgcagcacg atgcagaagt tcaaacggaa
     1081 cgattcagtt gcgctgttca tcgagcgcga cggcaagctg cagtatctcg ccttcgaagt
     1141 ggagtgacgc gcgcgcagtc agcaggcaat aatggtggtc tcatgaagat cactggaatt
     1201 gcggcttatc gagtggagct gccgttgcac gagggcagct ataaatggtc gggcggcaac
     1261 tccgtcgatg tgttcgattc gacggtggtg gagatttcga ccgacgcggg aatcacaggg
     1321 tacggagaga tctgtccgct ggggccggct tacttgccgg catacgcggc gggggcgcgc
     1381 gtgggcatcg cggagattgc gccgcagttg ttggggctgg accccacgga actgcaggta
     1441 atgaaccggc ggatggacca gacgctgcgc ggacacccgt acgtgaaatc ggcaattgac
     1501 atggcgtgct gggacattct cggcaaggcc agcaatcagt ctgtcgcaac gctgttgggc
     1561 ggctattacg agaaggactt tccgctctat cgcgcgattt cgcaggacac gccgaaggcg
     1621 atggcgaaga gcgtggggca ctatcgctcc gaaggctaca cgaagtttca gctcaaggtg
     1681 ggcggcaatc ccgatctcga tatggagcgg atacacgcgg cggcggcgca gttgaaggcg
     1741 ggcgatgtgc tgatcgcgga cgccaacacc gggtggacgc agcaccaagc gctgcgtgtg
     1801 gcggatgccg tgagaggcgt ggacgtttac atcgagcagc cctgctacag ctacgaagag
     1861 tgcctggcgg tgcggcggca cacggaccgg ccgttcgtgc tggatgaggt gatcgacggc
     1921 gtgcagatgg tgctgcgcgg gcacgcggag caggcgatgg atgtgatcaa cctgaagatc
     1981 agcaaagtcg gtgggttgac gaaggcgcgt cagatccgcg acttgtgcgt gtcgctggga
     2041 atcgcgatga cgatcgagga cacgtggggc ggcgacattg ttaccgcagc gattgcgcac
     2101 atggcgcaca gcacgccgcc ggagctgctc ttcacggcga ctgacttcaa cagctacgtg
     2161 acggtgagca tcgcggaggg cgcgccgcaa cggcgcgaag gacgcatgca ggcatcgcgc
     2221 gaacctggct taggcgtgcg gccgcgcatg gaagttctgg gcaagcccgt gcttcacttc
     2281 gcggcggatt cgacgtccat gaagtagtaa cagatcatgt ggcagacgat catgtgggcg
     2341 tcttcgatgc ggcccatatg agggatgccg acctgtatgt tgagctgcgc cataggcccg
     2401 agcttgccgc catcgcggcc ggtgagggcg atggtgcggc agccgacttc gtcggcgacc
     2461 tgcatggcgc gaaggacgtt gggcgaattg ccggagccgc tgatagccat gaagagatcg
     2521 ccgggctgcg cgaagttgcg gagcggctcg gcgaagacga catcgtagct gacatcgttt
     2581 gaatacgcag tgagggtggg cagcgaatcg cagagggcca tcacgcggaa gcgcgaggcg
     2641 cgattcatgc tggcgccttt caccatgtcg cagacgaagt gggacgcggt gctggcgctg
     2701 ccgccattgc cgcagacgaa gacggcgcgg ccggagtcgc gggtttcgcg gaaccactca
     2761 atggcacggt gcacggcctc cgtgtcgata cgctcgatgg cttcgagcag gtcacgtttg
     2821 tagtcgagag caaaagtcac agttggttca ccagggcgag cgcgccatag agcacgctgt
     2881 catcggccaa ttgggccgga actacgtcga tgcgagcggc cgaccacgaa gtgatttgcc
     2941 ggcgcagttc ggcgcgcagc gggtcgaaga gcaggccgcc agctttactg atccccccgc
     3001 cgatcacgat tcgggcgggg ttcaataaca tgatacaagc cttgagaccg agcgcgagat
     3061 cgacgacgta gcggcggacg aattcgggat cctgcatgag gtctttggcg ggcttgccgt
     3121 ggtcgcgttc gagccagagg ccgcagcaca tgcgctcgaa gcagccgaag ctgccgcaga
     3181 gacattcggg gccatcgggg cggatggtca agtggccgat ttcgccgccg taggaatcgg
     3241 cgccgcgcca gatttggcca ttgtcataga tgccgccacc gatgccggtg gagagcgtca
     3301 tgtagaagag cggagagcag ccgcggccgg cgccgtgtac ggcttcgccg agcgcgccga
     3361 cgttagcgtc gttatccata atgacgggga cgccgagaat ttcgcgtaga gcggcgggga
     3421 gatcgaaatc gcgccagccg ccgacgtgcg tggagagcat cacgcgctgc gaggcgaagt
     3481 tgacgggacc accgaaacct acgccgcagc gttcgaaggc gaaatcgcgc ttccactcgg
     3541 agtgaatttc ggcgatgcgg gcgagcatcc aatcgcggct accagcgcgg tcggtgggct
     3601 gcgagacgcg gcgaaccatt tggtcgccat cgaaggcggc gacggagaat ttagtgccgc
     3661 caatgtctat tgcgaggacg tgcatcgctg cgcgatctcc tcacggctgg ctacgccggt
     3721 gcctttctgc atgatggcga tggacgcggc gagattgccg aaggacgcag cttcggcggc
     3781 cgaggcgcca gccgcgagcg caagcgcgga gcccgcggag aagctatcgc cggcgccgca
     3841 gatatcgacg ggatcggcga caggttgcgt gcggacccac tcttcgcgat cggggtgaac
     3901 gagcaggacg ccatcgccgc cgtgggtaat catgagcaga tcggtggaga ggtgggcgcg
     3961 gagtgcggcg taatcgacgc ggccaaagag agcgccgcag gcggcttcgc cttcatgctg
     4021 attgggtttg gcgataacgt tgcgaaattc ggtgatgcgg gcgcgcgaat cgacgataaa
     4081 ggttttatga ggatggaggc gcgcgagttc gcaaagggcg tcgcgaaccg cgggcgtgac
     4141 taccccgccc tgccccgttt ccgcttgatc ggagatgatg acgacgtcga aatcggcgat
     4201 ggcctggtgg agcttggcga tgacttcgcg ctcgacactg gaatcgagcg gaacgatgtt
     4261 gatgaaatcg acgcgcggct gatcttcgac gccggcttgg cgattaatga gcttggtgta
     4321 ggtgaaggtc tgcatcgcgg aggtgcggac acagaagcgg gattcgatgc cgcgcgaatc
     4381 gagcgcgcgc gaaagctcaa atccaaaccc atcttcgccg ataacgccga gtacggagac
     4441 gcggttgcag ccgagagcag cgaggttgtt ggcgacggtg ccggcggcgc ccgcggtgac
     4501 ttcggttgcg actacgccga ggcgcgggat gccggtttcg cgcgagggtt cggatacggc
     4561 tggatcgtag gtgcaccagc gatcgagaca gacatcgccg acgacgagcg cggagagctt
     4621 gggaatggcc gcgaggattt cgtgaggcgt cataggcgat cgagcgcggc gcggcgcaac
     4681 gccggcagag tggcgcgatg gtcgccgcag aagtagaagc tttcgccacc atcggcgacg
     4741 gtgcggacga ggatggtttt gtagggtcgg aagtaatagg cggcttcggt cttggaacgc
     4801 tctttgtgga tgtcgccagc gagcggcatc agatcgaaga cggcggtggc gaaatgacgg
     4861 atttcgcggc cttgctggtg cgcgacgttg cgcgccatgg agagcgcttt caaataaact
     4921 tcggggccca tgacggcgga gccgaagcag agcaggacgc cggactcgag gcgttcgacg
     4981 gcgctggcga agatgaggaa atcgcggtag ctggcatcgc cgacggcggc gccgtcgcaa
     5041 ttgggatgtt cgtgaatgat gtcgtaaccg atgccggcgt gaacggtgac ggggatattg
     5101 aggcgccagg cagccgcgag aacgctgagt tcgatattgg gaaagccgct ggatg
//
LOCUS       DASZMC010000233         6591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4770810,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000233 DASZMC010000000
VERSION     DASZMC010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4770810"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1348
                     /gene="tolB"
                     /locus_tag="VG274_04645"
     CDS             38..1348
                     /gene="tolB"
                     /locus_tag="VG274_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HWE05973.1"
                     /translation="MAIVAAWVTVVVFLTVSGNPAGAALRVDVNQGNIQPLPIAIPDF
                     LGAQPGDATAGQQIAGVVRADLDRSGIFRPLDPRSFIEPIKDINVPPAFGNWRVINAQ
                     ALVTGMVAAQPDGRLRVDFRLWDVFGESQMLGLQYFTAPANWRRIAHMISDAIYQRIT
                     GEKGYFDTRIVFVSESGSLINRKKRLAVMDEDGANPIFLTRGDYLVLTPRFNPTAQMI
                     AYMSYVSSRPRVYLFDLETGRQQMLGDFPSMTFSPRFSPDGNKVAMTLEQRGNSDIYV
                     MDLRTRAVTRLTTDPAIDTSPCYSPDGSQMVFESDRGGSQQVYVMSAAGGNVHRISFG
                     GGKNGTPVWSPRGDLIAFTKQEGGTFRIGVMRPDGSGERMITDGWEDEGPTWAPNGRV
                     LMYTRTLRGGRGSQIWSVDVTGRNERRVQTPGDASDPAWSPLIQ"
     gene            1486..2013
                     /gene="pal"
                     /locus_tag="VG274_04650"
     CDS             1486..2013
                     /gene="pal"
                     /locus_tag="VG274_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWE05974.1"
                     /translation="MLSKSNPLKIAALAFVLVLAACTTKPKTAEAPPPEGPPPASTQN
                     QVESTVVPGSVQDFRVNVGDTVHFEYDKYDIKGQDRDLLQRQAQWLQKYAQVRVTVEG
                     HCDERGTREYNLALGARRANAVKEYLVSLGVPSARVDTISYGKERPICTESDESCWAQ
                     NRRGVTTITSGATTS"
     gene            2260..3219
                     /gene="ybgF"
                     /locus_tag="VG274_04655"
     CDS             2260..3219
                     /gene="ybgF"
                     /locus_tag="VG274_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HWE05975.1"
                     /translation="MSQDEFSLRILAARADASLPEGKAARLTARTGEMKVAQLFGESD
                     EEKAARQQHEQTQDSSIAALNQRLQELSDVEDSLRRLTGQVEQLDHRLTELNDRITRV
                     QKEFDYKLCALAAQQLGASSDSGDQTALPCNGVGQQTGTPPPGGGGAPPPASGGPIHL
                     APPPGVLGTIPRSDLGNLPQGPQSSQNQLASIDTRPQFESALNMLAKAQYDEARAAFR
                     GFADTYPRDDLAPQAVYWVGDIAYVQKDYAGAARAFAEELKKYPASPRAPESMLKLGQ
                     SLIAMNQKKEGCTALGALASRYPTASKSVADQALTARKAAGCR"
     gene            3254..4270
                     /gene="tilS"
                     /locus_tag="VG274_04660"
     CDS             3254..4270
                     /gene="tilS"
                     /locus_tag="VG274_04660"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWE05976.1"
                     /translation="MEAVERLGASWPAAAAVSGGGDSLALLLLLEEWARGGSRPAPIV
                     LIVDHRLKPHSGRDALKVASGAVARGLEAHVLSWRGRKPAANIESSAREARYRLMGDW
                     CQANGIACLFVAHSINDQAETFVLRLSRGSGVDGLAAMAPVSPFPLPGFEELRVARPL
                     LGESRSRLRAFLKLRGETWLEDEMNSDPRFARARLRAAWPALEQLGLSATRVAAAARH
                     LARARTALDLDAEELLARASRKMGEYVLADAVRLGGAAEEIGLRSLARLLMQVSGRHQ
                     RPRFESLESLFAAIRSGALGGGRTLHGCRIGRAPKRRALFGPDTLLISREAKRARGPG
                     GERN"
     gene            4438..6357
                     /gene="ftsH"
                     /locus_tag="VG274_04665"
     CDS             4438..6357
                     /gene="ftsH"
                     /locus_tag="VG274_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWE05977.1"
                     /translation="MNNLRNLALWIIIALLLVALFNMFQGTANRPASTTTNFTEFNQQ
                     VVQGAVKKVTIQGDQIKYELSNGQTYTTYAPNDPGLVSRMLEHNVGINVQPGDDGMPT
                     LMSVFINWFPMLLLIGVWIFFLRQMQAGGGKAMGFGKSRAKLLTERTGRVTFEDVAGV
                     DEAKEELKEIVEFLRDPQKFQRLGGRIPKGVLLVGSPGTGKTLLARAIAGEANVPFFT
                     ISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVGRHRGAGLGGGNDEREQ
                     TLNQLLVEMDGFESTEGVILIAATNRPDVLDPALLRPGRFDRHVVVPNPDLSGREKIL
                     RVHMRKVPLAPDVDPRVIARGTPGFSGADLANLVNEAALLAARRGRRVVTMAELEDAK
                     DKVLMGAERRSLAMTDEEKKLTAYHEGGHAIVALSVKGSDPIHKATIIPRGRALGMVM
                     RLPERDQLSITREKMLADICVAFGGRIAEELIFGHEKVTTGAMSDIEMATRMARAMVT
                     RFGMSDELGPLAYAENQDEVFLGHTIARQQNLSEATAQKIDQEIRTIIDNCYRRAHEI
                     LDQRVADLHTLAKGLLEYETLTGDEIVALLKGIPPVRAPYEEPEPQRGPGPSVPQAGR
                     PRGIAPVAPEPQPGR"
     gene            6354..>6591
                     /locus_tag="VG274_04670"
     CDS             6354..>6591
                     /locus_tag="VG274_04670"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWE05978.1"
                     /translation="MNGRPTLLGIVNVTEDSFSDGGRYLERGAALAHARAMAAQGADI
                     IDLGAAASNPDARPVSPELEIERLAPLVEILVRDG"
BASE COUNT         1210 a         2021 c         2161 g         1199 t
ORIGIN      
        1 gaaagacatg atgggagact agcttttgaa aaatggcatg gcgatcgttg ctgcgtgggt
       61 gaccgttgtg gtgttcctta ccgtgtcggg caacccggcc ggcgcggcgc tgcgggtcga
      121 tgtcaaccag ggcaacatcc agcctctgcc gatcgcaatt ccggatttcc tcggcgcgca
      181 gccgggcgat gcgacagccg gccagcagat cgcaggagtc gttcgcgccg acctcgaccg
      241 ctccggcatt ttcaggcccc tcgatccgcg ctcgttcatc gagccgatca aggacatcaa
      301 cgtgccgccg gccttcggca actggcgggt gatcaacgcg caggcgctgg tgacgggcat
      361 ggtggcggcg cagcccgacg ggcgtttgcg ggtggatttc cggctgtggg atgttttcgg
      421 cgagagccag atgctgggcc tgcaatattt caccgcgccc gcgaactggc ggcgcatcgc
      481 ccacatgatt tccgacgcca tctaccagcg catcaccggc gaaaaaggct atttcgatac
      541 gcgcattgtt ttcgtttcgg aaagcggttc gctcatcaac cgcaaaaagc ggctcgccgt
      601 catggacgag gacggcgcca atccgatctt cctgacgcgc ggcgactatc tggttctcac
      661 gccgcgtttc aatcccacgg cgcagatgat cgcctatatg tcctatgttt cgagccggcc
      721 gcgcgtctac cttttcgatc ttgagacggg ccgccagcag atgctggggg attttccgag
      781 catgacgttt tcaccccgct tctcgcccga cggcaacaag gtggccatga ctctcgagca
      841 gcgcggcaat tccgacatct acgtcatgga tctgcggacg cgggcggtga cacggctgac
      901 gacggaccct gcgatcgaca cctcgccctg ctattcgccg gacggcagcc agatggtgtt
      961 cgaatccgat cgcggcgggt cgcagcaggt ctatgtgatg agcgccgccg gcggcaatgt
     1021 tcatcgcatt agcttcggcg gtggaaagaa cggaacgccg gtctggagtc cgcggggcga
     1081 tctcatcgcg ttcacgaagc aggagggcgg cacattccgg atcggcgtga tgcggcccga
     1141 cgggtcgggc gagcgcatga tcaccgatgg atgggaggat gaagggccca cttgggcgcc
     1201 gaacggccgc gtcctgatgt acacgcgcac gctgcgcggc gggcgaggtt cgcaaatatg
     1261 gtccgtcgat gtcaccggcc gcaacgaacg ccgcgtccag acgccgggcg acgcatccga
     1321 tccggcatgg tcgcccttga tccagtaggt gttaactttt tagtatcggc ttaggacatt
     1381 ggggccaatc gggcccgcaa acgttgtagc gacggcgcgc gcgttcgtgc tgattgcgca
     1441 atctccgcgc atccggggat gcgccccttc cggagacggg gtcacatgct gagcaagtcc
     1501 aatccactga agatcgcggc cttggcgttt gtgctggttc tcgcagcatg cacgacgaag
     1561 cccaaaactg ccgaagctcc gccgccggaa gggccgccgc ccgcgtccac ccaaaatcag
     1621 gtggaatcga ccgttgtgcc ggggagcgtg caggatttcc gcgtgaatgt gggcgacacg
     1681 gtccatttcg agtacgacaa atatgacata aagggccagg atcgcgacct tctccagcgt
     1741 caggcccagt ggctgcagaa atacgcgcag gtgcgggtta cggtggaggg acactgcgac
     1801 gaacgcggca cgcgggagta caatctggca ttgggcgccc gccgggcgaa tgctgtgaag
     1861 gaatatctgg tcagcctggg cgtcccctcg gctcgcgtcg atacgatttc ctatggcaaa
     1921 gagcgtccga tctgcacgga gtccgatgaa tcctgctggg cccaaaatcg tcgcggcgta
     1981 acgacgatca cgagcggggc caccacttcg taggcttcga tcggccgccg gcgctgcgca
     2041 gaagtgctcg aaagcgtttt ctttcgcgcg gggatttccg gttcgcccta atttcggctt
     2101 agtattcaag tcccgttgga gagggccgcg tgaaacgcgc gatgggagcg ggcgccgatg
     2161 aggatgcgcg tatttcgttt tgggcgatgg tggccggcat tcgccggtat ggccattttg
     2221 tgcgctggca taagcgctga cgccggaact tcgcaaccgg tgtcgcagga cgaattcagc
     2281 ttgcgcatac tggctgcgcg ggcggacgcc agcctcccgg agggaaaagc tgcgcgcctg
     2341 acggcgcgga ccggcgagat gaaagtcgct cagctcttcg gcgaaagcga tgaggagaag
     2401 gccgctcgcc agcaacacga acagactcag gattcgagca tcgctgcgct caaccagcgt
     2461 ttgcaggaac tctccgatgt tgaggactcg cttcgccggc tgaccggcca agtcgagcag
     2521 ctcgaccacc ggctgacgga attgaacgac cgcatcacac gcgtgcagaa ggagtttgac
     2581 tacaaattat gcgcgctcgc ggcgcaacag ctcggcgcat cgagtgattc gggcgaccag
     2641 accgcactcc catgcaatgg cgtgggccag cagaccggta cgccgccgcc cggcggcggt
     2701 ggggcgccgc ctcctgcgtc tggaggaccg attcatctgg cgcctccacc cggggtattg
     2761 ggcacgatac cgcgcagcga cctgggaaat cttccgcaag ggccgcaatc gtctcagaat
     2821 cagctcgcgt ccatcgacac aaggccgcaa ttcgaatcgg cgctgaatat gcttgccaaa
     2881 gcgcaatatg acgaagcccg cgcggccttt cgcggtttcg ccgataccta tccgcgcgac
     2941 gacctggcgc cccaggcggt ctactgggtt ggcgacatcg cctatgttca gaaggattat
     3001 gccggagcgg cgcgcgcgtt tgcggaggaa ttgaagaaat atcccgccag cccccgcgcg
     3061 cccgagagca tgctcaagct cgggcagtcg ctgatcgcga tgaatcagaa gaaggaaggt
     3121 tgtaccgccc ttggcgcgct cgccagccga tatcccacgg cctcaaaatc cgtcgccgac
     3181 caggcgctca ctgcgcggaa ggcagccggc tgccgctgaa aagccgattt ccgcggcgga
     3241 attcgccgct ctcatggaag cggtggagcg gctgggagct tcgtggccgg cagccgctgc
     3301 ggtttcgggc ggtggcgatt cgctcgctct attgctgctt ctcgaagaat gggcgcgcgg
     3361 gggctcgcgt ccggccccca tcgtcctcat cgtcgatcat cgtttgaagc cacactccgg
     3421 acgtgacgcc cttaaggtcg cgtccggtgc cgtcgcccgc ggactggaag cccatgtcct
     3481 ttcgtggcgt gggcgcaaac ccgcggcgaa tatcgaaagc tccgcaaggg aggcgcgcta
     3541 ccggttaatg ggcgactggt gccaggctaa cggcattgcg tgtctttttg tggcgcactc
     3601 gatcaacgac caggcggaaa ccttcgttct ccgcctttcg agggggagcg gcgtagacgg
     3661 acttgccgcc atggcgccgg tttcgccttt ccctttgccc ggattcgaag agctgcgggt
     3721 cgcgaggccg cttctgggtg aatctcgcag cagattgcgc gcattcctga agcttcgcgg
     3781 cgaaacctgg ctcgaagacg aaatgaacag cgatccgcgg ttcgcccgcg cccgccttcg
     3841 tgcggcctgg ccggcactcg agcagctcgg cctctcggcg acgcgggtcg ccgccgcggc
     3901 gcggcacctc gcccgtgccc ggacggcgct ggatctggat gcggaggagt tgctcgcgcg
     3961 ggcatcgcgg aagatggggg aatatgttct ggcggatgcc gttcgactcg gcggcgcagc
     4021 ggaggagatc gggctgagat cgctcgcacg gcttctgatg caggtctccg gccgccatca
     4081 gcggccgcgc ttcgaaagcc tcgaaagcct cttcgccgcc atccgctcgg gagccctcgg
     4141 agggggccgg acgttgcacg ggtgtcgcat tgggcgggcg ccgaagcggc gtgccctttt
     4201 tgggccggac accttgctga tctcacgcga ggccaagcgt gcgcgtgggc cgggcggtga
     4261 gcgaaattga gccggtgctc cgcaaaccgc ctgccgttaa ttgcgatgca aatcagcgat
     4321 tgccgcatcc cggcaaagcc cttaacgttt tgatggctgg caaggcgcta tgaagttcct
     4381 atctaggtgg ataggttccc ccgcctggtg ccggatgggc cgcaggaaag gatggccgtg
     4441 aacaatctca gaaatctcgc gctttggatc attattgcgc tgctgctcgt tgccttgttc
     4501 aacatgtttc agggcacagc gaatcgtccg gcgtccacga ccacgaattt taccgaattc
     4561 aatcagcagg tcgtccaggg cgcggtcaag aaggtcacga ttcaaggcga ccagataaaa
     4621 tacgaactct cgaacggcca gacctacacg acatacgcgc cgaatgaccc cgggctcgtg
     4681 tcgcgcatgc tcgaacacaa tgtcggcatc aacgttcagc ctggcgacga cggcatgccc
     4741 accttgatga gcgtgttcat caactggttc ccgatgctgc tgctgatcgg agtgtggatt
     4801 ttcttccttc ggcagatgca ggcgggcggc ggcaaggcga tgggattcgg caagagccgc
     4861 gccaagctgc tcaccgaaag aaccgggcgc gtgacgttcg aggacgtggc aggcgttgac
     4921 gaggccaagg aggagctcaa ggaaatcgtc gagttccttc gcgatcccca gaagtttcag
     4981 cggctgggcg gccgcattcc gaagggcgtg ctgctggtgg gctcgcccgg aacaggcaag
     5041 acgcttctcg cccgtgccat tgccggagaa gccaatgttc cgttcttcac gatttccggc
     5101 tcggacttcg tggagatgtt cgtgggcgtc ggcgccagcc gggtgcgcga catgttcgag
     5161 caggcgaaga agaatgcgcc ctgcatcgta ttcatcgacg aaatcgatgc cgtcggccgc
     5221 catcgcggcg cgggtctcgg cggcggcaat gacgagcgtg agcagacgct caatcagctg
     5281 ctggtcgaaa tggacggttt cgaatccacc gaaggcgtga tcctgattgc ggcaaccaat
     5341 cgtcccgatg tgctcgatcc ggcgctgttg cggccgggca ggttcgatcg tcacgtcgtc
     5401 gtgcccaatc ccgatctttc cggccgcgaa aagattttgc gcgtgcatat gcgcaaggtg
     5461 ccgctggcac ccgatgttga tccgcgcgtc atcgcacgcg gcacgccggg tttttcaggc
     5521 gccgaccttg ccaatctcgt caacgaagct gcccttctgg cggcgcggcg cgggagacgc
     5581 gtcgtcacga tggccgagct cgaagacgcg aaggacaagg tgcttatggg cgccgagcgc
     5641 cggtcgctcg cgatgaccga cgaggagaag aagctcacgg cctaccacga aggcggccac
     5701 gcgatcgtcg cgctgagcgt gaaaggctcc gatcccattc acaaggccac gatcattccc
     5761 cgcggccggg cattggggat ggtcatgcga ctgcccgaac gcgatcagct ctccatcacg
     5821 cgggaaaaga tgctcgccga tatttgcgtg gcgttcggag gccgcatcgc cgaagagctc
     5881 atcttcggtc acgagaaggt cacgacgggc gccatgtcgg acatcgaaat ggccacgcgc
     5941 atggcgcgcg cgatggtcac ccggttcggg atgtcggacg aattgggacc gctcgcctat
     6001 gcggagaatc aggacgaggt atttctcggc cacaccattg cgcggcagca gaacctctcg
     6061 gaagcgaccg ctcagaagat cgaccaggaa atccggacca tcatcgataa ttgctaccgg
     6121 cgtgctcatg agattctcga ccagcgggtg gccgatcttc acacgctcgc aaagggactg
     6181 ctcgaatacg aaacgctgac gggcgacgaa atcgtggcgc tgctcaaggg tatcccgccg
     6241 gttcgtgcgc cgtatgaaga gccggagccg cagcgggggc cggggccgag cgtgccgcag
     6301 gccggccgtc cgcgcgggat cgctcccgtc gcgccggagc cgcagccggg ccggtgaacg
     6361 ggaggcccac gcttctcggc atcgtaaacg tcaccgaaga ttccttttcg gacggtggac
     6421 ggtatttgga acgcggcgct gcgctggcgc acgcgcgcgc tatggccgcg caaggggccg
     6481 atatcatcga tctcggcgct gcagcctcca atcccgatgc gagaccggtc tccccggagc
     6541 tcgagatcga acgcctcgcg ccgctcgtcg aaattctcgt gcgcgacggg c
//
LOCUS       DASZMC010000234         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4770965,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000234 DASZMC010000000
VERSION     DASZMC010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4770965"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..2431
                     /locus_tag="VG274_04675"
     CDS             17..2431
                     /locus_tag="VG274_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HWE05979.1"
                     /translation="MLAAADFNGDGHQDLAVAELGPSDAGLLLGNGDGTFQADQENPA
                     GLPAASIAAGDLNGDGNPDLVVGSYLPGGTPAQFSTLINSAAPTTSTLSDPTFAAATG
                     GNVDIPFNSEQVLQEPSGKILTVGHQGSVAGDTSQGVIEQLNADGTADNTFGAASLVT
                     TPMSSNDAFYSAALQPNGQIIAVGSSGADFLVARYNADGSLDTTFGSSGFATADFGAD
                     GAAVASSVAIEPDGSIVVGGQAGSVFALARFTAAGALDSQFNPAGADDATFPAGGERT
                     ISTGSAGSISQILIEGDGSILGVGSAGTTSAGGSFFLSIAVLRLNADGSNDPTFGTGG
                     ILTIAQLHSQDSTGAAITPAIGLQPGGSILVAGEAGTQLGFAAVRLSSSGTLDENFGG
                     PFQNDRANAVAGTSFGGANDDVNSIQVNPTDGEFVLTGTTNAGGIPQIAVAAFNPDGT
                     PNTAAFPPNGQTTMSVPSLLGESLVKAPGTGGSKIVESVRPFAFHPQNTDFSFATFQN
                     DGKLLVGASGASDSTLRRIQPFTATVTPIGAFGKIGRKNVKLPAFTLADETSVTIILT
                     GGSGTASHVGNQVQLTLSGSVNVTIRTKGGSNSLSLSGVSETGNLRSFNAPTATLAGT
                     LSTSGTIGQLTLAAISGVVISAGGISNLKAGNISGSVSCAGVLAIAKVTVLSGTIAAA
                     QIRNLTAGNLSHATILAGANLGSDGQLGGAGAAADTYGAGLIQSLHVTGTIVDSFIGA
                     GVDPVDGIFGNGNDVLVGTKSLVRALSVKLSVDSLTRFEAGGFPKIVHIRGKVITASD
                     PRFVVL"
     gene            complement(2943..>3791)
                     /locus_tag="VG274_04680"
     CDS             complement(2943..>3791)
                     /locus_tag="VG274_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05980.1"
                     /translation="MILQPEHFAAVRINWDDKAANLRAIARELNVGIGSIALLDDNPV
                     ECEWIREQIPEVTVIELPAEPSLYAEIVRNAAVFERLQLTEEDQARGKFYADQRQRAA
                     LEQSATSLEDFYRTLQMRARVELVNERTLARAAQLTQKTNQFNLTTRRYSEQQMAEMI
                     ASPTWRAFTIEVTDRFGDNGIVGVAILRFADDACEIDTFLLSCRVIGRTIETAFLSTI
                     AADAARQGAKKLTGWFIPTKKNAPSAGFYQQHGFTKTQTSEGAALWELVLAQKSIFCP
                     LWIIVE"
BASE COUNT          733 a         1207 c         1105 g          746 t
ORIGIN      
        1 gggcgcaact cccttcatgc tggcggcggc ggatttcaac ggcgatggcc atcaggacct
       61 ggccgtcgcg gaactcgggc ccagcgacgc cggcctgctc cttggcaacg gcgacgggac
      121 gttccaggcc gaccaggaaa atcccgccgg ccttcccgcg gcttctatcg ccgccggtga
      181 tctcaatggc gatggcaatc ccgacctcgt cgtcggcagc tatctgcccg gcggaacgcc
      241 tgcgcagttt tcgacgctca tcaattcagc ggcgcccacg acgtccacgc tctccgatcc
      301 gaccttcgcc gccgccaccg gtggcaatgt ggacatcccc ttcaactccg aacaagtgtt
      361 gcaagagccc agcgggaaga tcctgactgt cgggcaccag ggaagcgtcg ccggcgatac
      421 ttcgcaaggg gtgatcgaac agctcaatgc cgatggaacc gctgataaca cattcggcgc
      481 cgccagcctg gtgacgaccc cgatgtccag caatgatgcc ttctatagcg ccgcgcttca
      541 gcccaatggg cagattatcg cggtcgggag cagcggggcg gatttcctgg tggcgcgata
      601 caacgccgat gggtcgctcg ataccacgtt tggcagcagc ggttttgcaa ccgccgactt
      661 cggcgccgac ggcgcggcgg tggcatcgtc agtcgcaatc gagccggatg gatcgattgt
      721 ggtcgggggc caggcgggga gtgttttcgc tttggcgcga ttcaccgccg ccggcgccct
      781 ggacagtcaa ttcaacccgg cgggcgcgga tgatgcgaca tttcccgccg gcggagagcg
      841 caccatttcc accggctccg cagggagcat ctcgcagatt ctgatcgaag gagacggcag
      901 catcctcggc gtcggctcag ccgggacgac ctcggcgggg ggatctttct tcttgagcat
      961 tgcagtcttg cggctcaatg ccgatggaag caatgacccg acatttggca cgggcggaat
     1021 cttgacgatc gctcagcttc actcacagga ttccaccggc gccgcgatca cgccggcaat
     1081 cgggcttcag ccgggtggca gtattctggt ggctggtgaa gcgggcacgc agctgggatt
     1141 tgccgcagta cgactgagct ccagcggcac gctcgatgag aatttcggag gcccttttca
     1201 gaacgacagg gccaatgccg tcgccggcac cagtttcggc ggggccaacg acgacgtgaa
     1261 ctccatccag gtcaatccca ccgatggcga attcgtgctc accggcacca cgaatgccgg
     1321 cggcatccca caaatcgccg tcgcggcgtt caaccccgac ggcacgccca acaccgccgc
     1381 gtttccccct aacggccaaa cgacaatgtc cgtcccgagc ttgctcggcg agtcgctggt
     1441 taaagctccg ggaacgggcg gatcgaaaat cgtcgaatcc gtccgcccgt tcgcgttcca
     1501 cccgcaaaat accgattttt ctttcgccac tttccagaac gacggcaagc tcctggttgg
     1561 cgccagcggc gcatcggatt cgacgctccg gcgaatccag ccgttcaccg cgaccgtgac
     1621 gccgatcggc gccttcggaa aaatcggccg caagaatgta aaactgcccg cctttaccct
     1681 tgccgacgaa acatccgtca ccatcatcct caccggcggg tcgggcacag cctcccacgt
     1741 cggcaaccaa gtccagttga ccctcagcgg ctcagtcaat gtcaccattc gcaccaaggg
     1801 cggcagcaat tccctgagcc tctccggcgt cagcgaaacc ggaaatctgc gatccttcaa
     1861 cgcaccaacc gccacgctgg ccggcacgct gtccacctcc gggaccatcg gccagttgac
     1921 cctcgccgcc atcagcggcg tcgtcatctc cgccggcgga atcagcaatc tcaaagccgg
     1981 aaatatcagc ggctccgtct cgtgcgccgg tgtcctggca atcgcgaaag taaccgttct
     2041 gagcggaacc atcgccgcgg ctcagatccg taacctgacc gctggcaacc tgagtcacgc
     2101 cacaattctc gccggcgcca acctgggatc ggatggacaa ctcggcggcg cgggcgccgc
     2161 cgccgacacc tacggcgcgg gcctgatcca atccctgcac gtgactggca cgatcgtcga
     2221 ttcgttcatc ggcgccggcg tggatcccgt cgatggaatt ttcggaaatg gcaatgacgt
     2281 gctggtcggg acaaagagtt tggttcgtgc tctgtcagtc aaactcagcg tggattcact
     2341 cacgcggttc gaagccggag ggttcccgaa gatcgtgcat atccggggca aggtaattac
     2401 agcaagtgat ccgcggtttg tggtcctcta gtctagacta gagcagttat caacttgcta
     2461 cctcgattag cgcgtttgaa aaaccgcgag agacacgact atccaaagta gcgcactctt
     2521 ggcggacgag ttcgcggagc gcttggacgg cgaacccgtt tctctcgagc ctgctccccg
     2581 aaacgcgtcc gccaaggcgg accctacggg aacgttttca gagcaggagg ctttgtcgac
     2641 gaagcatctt gccgtggact attgctgttg aaccccgcgg gaagattctt cgtcggcaaa
     2701 gccttctcag aataacgcgc acaaactccg gtaacaaaac cgcactcgtt tcgtcagagc
     2761 ggggttggtg taagagccgt tcgtcgagct gcgcccctcg cgagaatggc ccctcacccc
     2821 aaccctctcc cccgagtacg ggggagaggg ggccggagtt tatcaccaca atcatcattg
     2881 atcatgggtg tgactttgcg tgaacgaacg ccataacgac cgcggactgg ttcattcacg
     2941 cttcactcaa cgatgatcca cagcgggcaa aaaatcgact tctgggcaag caccagttcc
     3001 caaagggctg cgccctcgct tgtctgcgtt ttcgtgaatc cgtgctgttg atagaacccc
     3061 gccgagggcg catttttttt tgtcgggata aaccatcctg tcagtttctt cgccccttgg
     3121 cgtgctgcgt cggcggcgat tgtcgagaga aaagcggttt cgatcgttcg gccgattacc
     3181 cggcaactga gcaggaatgt atcaatctca catgcgtcat cggcgaaccg caggattgcg
     3241 acgccgacga ttccgttgtc gccgaagcga tccgttacct cgatcgtgaa ggcccgccat
     3301 gttggcgacg cgatcatctc cgccatctgc tgctcgctgt atcggcgggt ggtgagattg
     3361 aactgattcg tcttctgtgt cagttgcgcc gcgcgggcca atgttcgctc gttgaccaat
     3421 tcgactcttg cgcgcatctg aagcgtgcga tagaaatctt caagagatgt cgcgctttgc
     3481 tccagcgcag cgcgctgccg ttggtcggcg tagaactttc cgcgggcctg gtcttcttcg
     3541 gtaagctgca atcgctcgaa aaccgcggcg ttccgcacga tctcggcata cagcgacggt
     3601 tctgccggca attcaatcac tgtcacttcg gggatctgtt cgcgaatcca ctcgcactcc
     3661 accgggttgt catcgagcaa tgcgatggag ccgatgccga cgttcaattc cctggcaatc
     3721 gcccggagat tagcggcttt gtcgtcccag ttgatgcgga cggcggcgaa gtgttcgggc
     3781 tgcaggatca t
//
LOCUS       DASZMC010000235         5793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5315570,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000235 DASZMC010000000
VERSION     DASZMC010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5315570"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..946)
                     /locus_tag="VG274_04685"
     CDS             complement(23..946)
                     /locus_tag="VG274_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWE05981.1"
                     /translation="MPKRARSSKPSQSASNDNVTRSGRPAWQGHLRLSLVSCPVALIN
                     ATTRASDISFHLINPDTNNRIRMVSTDPDTGPVNRSDLVKGYEVSKGEYVLLTPEDLE
                     SVKLETTRTLDIEHFVNADDIDRLYWEAPYYLVPDGAEGIEAYSVIHEAMLRSNQLAL
                     GRVVMHTRERLLAIEPRGKGMVAYTLRMANEVRDPDEAFRNIPDRKPDKQMTDIALKI
                     ISQQEGAFEPGKFVDRYEEALKALIREKQKGHKPVRAPEPEDTNVIDLMAALKKSLGQ
                     EPPAKPKQSASPAKPKRGAATQAAKTGRKRA"
     gene            1049..1336
                     /locus_tag="VG274_04690"
     CDS             1049..1336
                     /locus_tag="VG274_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05982.1"
                     /translation="MRQGVTFQLEGGIGMGKVTDLDSVRHHGKPSLIRTPSVPLGTDV
                     RKARAHSYRLRGEELRVIAEDVISPDTARTLLNLAETYDQMASTLDNMQTP"
     gene            complement(1396..1929)
                     /locus_tag="VG274_04695"
     CDS             complement(1396..1929)
                     /locus_tag="VG274_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWE05983.1"
                     /translation="MNFRIACAALALAFVPPAALAAQGADDFRHKAMASDAFEIASSK
                     IALGQSRNAGVKAFARMMIHDHSLTIKHLLARSRMSMEDVDAKIAPGPDGKHKASDLV
                     DQSHADTLDKLGAEKGKDFDSDYMSDQVSGHKDAVSLFEDYARSGDDRSLKLWARKTL
                     KTIKMHLAKAEALDRRM"
     gene            2229..2702
                     /locus_tag="VG274_04700"
     CDS             2229..2702
                     /locus_tag="VG274_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05984.1"
                     /translation="MRCNKEQSGPSGGTAGPSASVAGENTLASELDQLFRTDRYVADP
                     TIEYHRSEAARIPLRSSSRSGVPAEDRAYLATLVRTHTGIDAGQADERVNRLIAQSKD
                     ELHRARMAAVMQAFLIAAALLAGAAAAWFSAAEGGRDRERGTIPVWNWSLRSPRA"
     gene            complement(2796..3032)
                     /locus_tag="VG274_04705"
     CDS             complement(2796..3032)
                     /locus_tag="VG274_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05985.1"
                     /translation="MDVIGRTDGRETSIKPTPALHSRRQREARARGYRVRAEELLAIS
                     EDVLRDETRTTLRGIAENYEFMASALENLQGLDI"
     gene            3374..4351
                     /locus_tag="VG274_04710"
     CDS             3374..4351
                     /locus_tag="VG274_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HWE05986.1"
                     /translation="MPPIIAVGASQGGVNAVRGLTSGLPADFPAAILIVVHIGRGPST
                     LPSILNRAGRLRASHARDGDRIEPGHILVAPPNRHLLLADGKVELSHGPRENWARPAI
                     DPLFRSAAEAYGQGVIGVVLSGGLNDGTVGLYEIKRRGGIAVVQDPSDAEAPSMPRSA
                     LDNVAVDYCVPLTEIPGLLSSLAGDLKKRPRSHTESGVHVMEHAGRKLQHPVAQTCPD
                     CGGAMREERLGTLTQFRCHIGHVMTAETLAAVQVEILERDVSAVLRTLNERAELCLAL
                     ARAYGKKGDAETRNLWTNAAEEAKNREDAIRAMARAEWIRPESAEPAAG"
     gene            complement(4373..4690)
                     /locus_tag="VG274_04715"
     CDS             complement(4373..4690)
                     /locus_tag="VG274_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05987.1"
                     /translation="MIVALDIKDSLEDGGAEIIGPSYTPSDALDAATHAEIAAAVLDL
                     RLGRESVGPVARVLAQRNIPFVFYSGQPAGDPVRAEWPDVRLISKPATPRDLVEAVAA
                     LLR"
     gene            complement(4782..>5793)
                     /locus_tag="VG274_04720"
     CDS             complement(4782..>5793)
                     /locus_tag="VG274_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HWE05988.1"
                     /translation="IDGVVVTFVDISQRRRAEDALRDSEQHLRQQLRLIELSHAPIFI
                     WDFEKGIRQWNRGSEDLYGFTKEEAVGNDKEVLLKTQVPGSSFDALKKELLEKGTWNG
                     ELVQTSKAGRPLRVESQVELITVGERRYVLESARDVTQKKAWEAHQKLLLGELTHRVK
                     NILAVVQGMVHQTWRTGSREQFMDRLDGRLAGLSRSHSLLVESDWRGADMRKLVESQL
                     APYAGDSGRVRMVGEPIALPADIATPFGLVLHELATNAVKYGALSGSRGRIDLNWEVN
                     GNEAPRLRFTWQERDGPRVKEPTANGFGSQLIKKGLAGAKVNYEFPVQGIECTIELPL
                     PG"
BASE COUNT         1060 a         1851 c         1716 g         1166 t
ORIGIN      
        1 tgccggacga gcaaccacca gcctatgctc gcttgcgtcc cgtcttggcc gcttgagtag
       61 cggcaccgcg cttcggcttc gcaggtgatg ctgactgctt tggcttcgcc ggtggctctt
      121 gtcccaggct cttcttcagc gccgccatca gatcgatgac gttggtatct tccggttccg
      181 gggcgcgcac gggcttgtgt cccttctgct tttcgcggat cagggctttg agcgcttctt
      241 catagcggtc gacgaatttg cccggctcga acgcgccttc ctgctggctg atgatcttga
      301 gcgcgatgtc ggtcatctgc ttgtcgggct tcctgtcggg aatgttcctg aaagcttcgt
      361 cgggatcgcg cacctcgttg gccatgcgca acgtataggc gaccatcccc ttcccccgcg
      421 gctcgatggc gagcagcctc tcgcgcgtgt gcatgacgac acgcccgagc gccagctgat
      481 tggagcgcag catcgcctcg tgaatcaccg agtaggcttc gatgccttcc gcgccgtccg
      541 ggacgagata ataaggcgcc tcccaataga ggcggtcgat gtcgtccgcg ttgacaaagt
      601 gttcgatatc gagcgtgcgc gtcgtctcca gcttcacgct ctccagatcc tccggggtga
      661 gcagcacata ttctcccttc gaaacctcgt agcccttcac cagatcggag cggttgaccg
      721 ggcccgtgtc gggatcggtc gagaccatcc ggattcggtt gttggtatcg gggtttatga
      781 ggtggaagga aatgtcgctc gcccgcgtcg tcgcgttgat cagggccacc gggcacgaaa
      841 ccagcgacag cctcagatgc ccctgccagg ccggccggcc gctccgggtg acattatcat
      901 ttgacgcgct ttgcgagggc tttgatgagc gggctctctt cggcatggca ggcctccctt
      961 gatccgggat tcaaactcgc cgccccgcgg ttcgttcaaa acggctcttc gcctcttgcg
     1021 gcgtgtccgg tttggggaga gtgttggcat gagacagggc gtcacatttc aactcgaagg
     1081 cggcattggc atgggcaagg tcaccgacct cgacagcgtc aggcatcacg gcaaaccatc
     1141 cctgatccga acgccgagcg tgcccctcgg cacagacgtg cggaaagcgc gcgcgcatag
     1201 ttatcgtctc cgcggtgagg aattgcgcgt aattgccgaa gatgtgattt caccggacac
     1261 cgcgcgcaca ctcctcaatc ttgccgagac ctacgaccag atggcgtcga cgctggacaa
     1321 tatgcaaacg ccgtaaactg ccgtgcgcgt cttgcaattc gcgtggccgg ttatgcgaag
     1381 tgcattccgt cggcctcaca tcctcctgtc gagcgcttct gccttcgcga ggtgcatctt
     1441 gatcgtcttc agcgtcttgc gagcccagag tttcagactc ctgtcatcac cactcctggc
     1501 ataatcctcg aataacgaga ccgcgtcctt gtggccactg acctggtcgc tcatataatc
     1561 ggaatcgaaa tcctttccct tttcggcgcc gagcttgtcg agcgtatcgg catgcgattg
     1621 atcgacaaga tcgctcgctt tgtgcttgcc atcgggaccg ggtgcgattt tcgcatcgac
     1681 gtcttccatc gacatgcgcg agcgggccag caagtgtttg atcgtcagcg aatggtcatg
     1741 gatcatcatt cttgcgaacg ccttgacccc cgcattccgg ctctggccaa gcgcgatctt
     1801 cgatgacgcg atctcgaagg cgtcgctcgc catggcctta tgccggaaat catcagcgcc
     1861 ctgcgccgcc agggctgcag gcggcacaaa cgcaagcgca agcgctgcgc atgcaatgcg
     1921 gaaattcatg gattcctccc gttatggcgt acctcatgtg aacgccggac gccaaaacag
     1981 agttgcgggg cgtaccggct tttcaacgac tttcgggcgc gatcgcggaa aggcgggtcg
     2041 cgaatacccc cgatcccgag cagctatgag aggcgctggg ctctgcaatc caccgatcgg
     2101 catggcgcgg acggagcgat ccgtatttga tcccctatca tatacaattc ggtaaaccgg
     2161 ccccgcaatt gctcttgcgc caccgcaacc gtgtttgaca tcgcgccact cgccttcgcc
     2221 ttcccgttgt gcgctgcaac aaggaacaat cagggccatc gggaggaacg gccggcccat
     2281 cggcgtccgt cgccggcgag aatacgcttg cctccgaact cgatcagttg ttccgcaccg
     2341 accgctacgt cgccgatcca accatcgaat atcaccgttc cgaggcggcg cgcatccctc
     2401 tcaggagttc gagccgcagc ggagtgcctg ccgaggaccg cgcctatctg gcaacgcttg
     2461 ttcgcaccca caccggcatc gatgcgggcc aggctgatga acgcgtcaac cgtctcatcg
     2521 cgcagtcgaa ggatgaattg caccgggcgc gcatggcggc cgtgatgcag gcgttcctga
     2581 tcgccgctgc tcttttggcg ggagctgcgg ccgcctggtt ttctgccgct gagggcgggc
     2641 gagaccgcga gcgaggcacg attccggtct ggaactggtc gctgcgcagc ccgcgtgcct
     2701 gagtggcgcg ttcgcaaata ttgcgataat ccgccagccc ggctctgctc aggatgccga
     2761 tcacacttcg cagaagtccc gaaatcgtga tcggatcaga tgtccagtcc ttgcaagttc
     2821 tcgagcgcgg acgccatgaa ttcatagttc tcggcgatgc cgcgcagggt cgtccgggtt
     2881 tcatcgcgca gcacgtcttc cgagatcgcc aatagttctt ccgcgcgcac gcgatagccc
     2941 cgggcgcgcg cctcgcgctg tcttctgctg tgcagagcag gcgtcggttt gatcgaagtt
     3001 tcgcgtccgt cggtccggcc gattacatcc atgttgtcct ccaaagacag acaagggacc
     3061 ccccacggca gcttacaatc ccaaactttg cgacaaacat caacaggcgc cgcaataccg
     3121 gcactcatgg gtccgacaaa ctcgccatca gagttcgaca cgccgaagct cgcgcgcgcg
     3181 gcgcaacagg cacgctcaca atgcggatgc aaattgccgg atgtctttcg acatctcgtt
     3241 aacgcaatcc gcgcgcgaaa agcggcgacg cgactccgca cggcggcgac tcaccgcgga
     3301 agcggcacga gcgaaccgag gcagccaatc ggcgccggga tcgttgtcta tcggcgcggc
     3361 gggagcaacg taaatgccgc ctatcattgc agttggggca tcgcagggcg gggtcaacgc
     3421 cgttcggggg ctgacatccg gtctgccggc cgattttccg gcagctattc tgatcgtcgt
     3481 acatatcggc agaggtccga gcacgctgcc gtcgatcctg aaccgcgccg gcaggttgcg
     3541 cgcgtctcac gcgcgagacg gggaccgtat cgaacccggc cacattctcg tcgcgcctcc
     3601 gaatcgccac ctcctgctgg ccgacgggaa ggtggagctg tcgcacggcc cgcgcgagaa
     3661 ctgggcccgt ccggcgatcg atcctctctt cagaagtgca gcggaagcct atggacaggg
     3721 tgtcatcggc gtcgttctct cgggcggcct caacgacgga acggtcggtc tgtatgagat
     3781 caagcgccgc ggcgggattg cggtggtgca ggacccgtcg gacgccgaag ccccttcgat
     3841 gccgcgcagc gcgctcgaca acgttgcggt cgattactgc gttcccctta cggaaattcc
     3901 cggcctcttg agcagcctcg ccggagatct gaaaaaacgt ccgcgcagtc acacggaatc
     3961 cggagtacat gtcatggaac atgcgggccg caaactgcag cacccggttg cgcagacctg
     4021 tcccgattgc ggcggcgcca tgcgcgaaga gcgactgggg accctgactc aattcagatg
     4081 ccacatcgga catgtgatga cggctgaaac tctggcggcc gttcaagtcg agatacttga
     4141 gcgcgatgtc tcggcagtgc tgcggacact gaatgaaaga gcagagcttt gtctggcgtt
     4201 ggcccgcgcc tacgggaaaa agggtgacgc cgaaacccga aatctctgga cgaacgcggc
     4261 ggaggaagca aagaaccgcg aagatgccat tcgggcgatg gcaagggcgg aatggatacg
     4321 gcccgaatct gccgaacccg ccgccggatg accggcgagc ttttcgttcc tgctacctca
     4381 acagggctgc gactgcttct accaaatccc gcggcgtagc aggcttggag atcagccgca
     4441 catcaggcca ttcggcgcga accggatcgc cagcaggctg tccgctgtaa aagacgaagg
     4501 ggatattgcg ctgcgccagg acgcgcgcga cgggtccgac agattcccgg ccgaggcgga
     4561 gatcgagaac cgctgcagcg atttcagcgt gggttgccgc atccagtgca tcagaaggag
     4621 tgtaggaagg tccgatgatt tccgcgcctc cgtcttcgag gctgtccttg atgtccagag
     4681 cgacgatcac ctcgtcttcc acgataagga tacgggcgcc aaccaaaccg tccctccgag
     4741 cagaatccga ttcggcccgc gcagtcgctg ccgccgggga atcatcccgg gagcggcaac
     4801 tcgatggtgc attcgatgcc ctggacagga aattcataat tgacctttgc ccccgccagc
     4861 ccctttttga tcagctgact gccaaacccg tttgcggttg gttccttcac tctcggtcca
     4921 tctcgctctt gccaagtgaa cctgagccgc ggcgcttcgt tcccgttgac ctcccagttg
     4981 aggtcgatgc ggcctctcga tccggaaagc gcgccatatt tgaccgcgtt cgtcgccagt
     5041 tcgtgcagca ccagtccgaa gggcgtcgct atatcggctg gcagcgcaat gggctcgccc
     5101 accatgcgga cccgacccga atcgccggcg tagggggcca gctgactttc caccagcttg
     5161 cgcatgtcag ctccgcgcca gtccgattcg accagcaaac tgtgtgaccg cgacagacct
     5221 gcaagccggc cgtccagacg atccatgaat tgctctcgcg agcccgtgcg ccatgtctgg
     5281 tgaaccatcc cctggacaac ggcgagaatg ttcttgacgc ggtgcgtcaa ctcgccgagc
     5341 aggagcttct ggtgcgcttc ccaggctttt ttttgggtga cgtcccgcgc gctttcgagg
     5401 acatagcggc gttcgcccac cgtgatgagc tcgacctggc tctccaccct gagcggtcgc
     5461 cccgctttgg atgtctgaac cagctcgccg ttccaggtgc ctttttcgag aagttccttt
     5521 ttcagcgcgt cgaacgaaga accgggaacc tgagttttca gcagtacttc cttgtcgttg
     5581 ccgacagctt cctccttcgt gaaaccgtac agatcctcgc ttcctctgtt ccattgccga
     5641 atccccttct cgaaatccca gatgaagatc ggcgcgtgcg acagctcgat caggcgcaat
     5701 tgctgccgca ggtgctgctc gctgtcacgc agggcgtcct cggcgcgccg ccgctggctg
     5761 atatcgacga acgtcaccac caccccgtcg atc
//
LOCUS       DASZMC010000236         6531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3948708,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000236 DASZMC010000000
VERSION     DASZMC010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3948708"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1158)
                     /gene="pheS"
                     /locus_tag="VG274_04725"
     CDS             complement(82..1158)
                     /gene="pheS"
                     /locus_tag="VG274_04725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWE05989.1"
                     /translation="MSDIDSLERRLLDAIGNAGDEAALETVRVSALGKKGSISELLKS
                     LGAMSPEERREKGPLFNGLRDRAAAAIAERKTVLARSKLDARLAFERIDVTLPPRPEP
                     RGTIHPVSQVQDEITAIFADLGFAVAEGPDVEFDDYNFTKLNIPPDHPARQMHDTFYV
                     ARQTDGARHVLRTHTSPVQARVMLSQKPPIRVIVPGRTYRRDSDATHTPMFHQVEALV
                     IDEETHLGHLRWVVEEFCSAFFEVERIELRARPSYFPFTEPSVEWDMRCDRSGGQIKF
                     GTGTDWLELGGSGMVHPHVLEMCGIDPERYQGFAFGFGIDRMAMLKYGAPDLRAFFES
                     DLRWLRHFGFAALDLPTLDGGLSR"
     gene            complement(1221..1568)
                     /gene="rplT"
                     /locus_tag="VG274_04730"
     CDS             complement(1221..1568)
                     /gene="rplT"
                     /locus_tag="VG274_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWE05990.1"
                     /translation="MSRAPRAVPSHLRRKRVLKQAKGFRGRRKNNITTANAAVDRSLQ
                     HAYIGRKEKKRNFRALWIQRINAACRLNGLTYSRFISGLARAGIELDRKILADLAVRE
                     PDAFKALAEQARA"
     gene            complement(1583..1783)
                     /gene="rpmI"
                     /locus_tag="VG274_04735"
     CDS             complement(1583..1783)
                     /gene="rpmI"
                     /locus_tag="VG274_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWE05991.1"
                     /translation="MPKLKTKSGAKKRFKFTAKGKLKHGQAGKRHGMIKRSNEQIREL
                     RGTTVMSASETQRVRRWMPYSA"
     gene            1983..2594
                     /locus_tag="VG274_04740"
     CDS             1983..2594
                     /locus_tag="VG274_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE05992.1"
                     /translation="MKMLNEFRTFGARLSVRTAVLVAVLVFVCASPGAARSAPASDDV
                     QGVANGFYAVYGTFRPSDGIPDAKGRAKYEPFISPALDRLLMEGEAAERRFVNATKHM
                     SPPLIEGDLFTSNFEGATSWSVGPCETAAATAHCKVALGYRGGPGEDSKPVNWTDTIY
                     LVRNGAGWRVDDIAYGAPWAFGNKGRMTQTLQSAIRDGNNATQ"
     gene            2630..3037
                     /gene="msrB"
                     /locus_tag="VG274_04745"
     CDS             2630..3037
                     /gene="msrB"
                     /locus_tag="VG274_04745"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWE05993.1"
                     /translation="MNRNEFAESRSDAEWRARLTDDQYRVLREHGTEPAGSSPLNAEK
                     RTGVFRCAGCGMELFESDTKYESGSGWPSFTAPRDQAVGTSRDASHLMERTEVHCARC
                     GGHLGHVFEDGPRPTGQRYCMNGAALTFEPAKE"
     gene            3074..3751
                     /locus_tag="VG274_04750"
     CDS             3074..3751
                     /locus_tag="VG274_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWE05994.1"
                     /translation="MADPLQTAEQIAQVAIPIIVAITLHEAAHGYAARHFGDDTAARA
                     GRISLNPLRHIDLFGTILLPGLLLLSHAGFVFGYAKPVPVNFAALRNPKRDMVWVAAA
                     GPGMNVLLALLSGLALFTMLQFGGTAHKMALEFFWTSVEINLVLAVFNLLPVPPLDGG
                     RVAVGLLPQPLSLALAQLNRFGMLLLIAIFILLPLIGINLFHYLVQIPVEFLERPLLS
                     LLGIPAQ"
     gene            complement(3791..4186)
                     /locus_tag="VG274_04755"
     CDS             complement(3791..4186)
                     /locus_tag="VG274_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05995.1"
                     /translation="MIGLDTNVLVRYLAQDDPVQSPKAVDLIERRLSEENPGFVSVVS
                     MAETAWVLERAYDLADEEVAACIERILQTAVLVVESEQQVFTAMIALKDGRGAFSDAL
                     ILALGDKAGCARTVTFDRKALRLPGFALP"
     gene            4581..4823
                     /locus_tag="VG274_04760"
     CDS             4581..4823
                     /locus_tag="VG274_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWE05996.1"
                     /translation="MRSVNLAEAKAHLSELVERAAAGEVVCIMRRGKPVAKLTGLGTK
                     RKRVDAAALRTLTAPMPKQKQTAGELVRRMRDKDRY"
     gene            4820..5095
                     /locus_tag="VG274_04765"
     CDS             4820..5095
                     /locus_tag="VG274_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE05997.1"
                     /translation="MIHYLDTSLLVAAFTNETRTQEMQSWLGRQTPGSLAISEGVVTE
                     FSAALSIKLWTSQIAAAHRAGALSMFGRTADESFVLLPNPVESSPSL"
     gene            complement(5488..>6531)
                     /locus_tag="VG274_04770"
     CDS             complement(5488..>6531)
                     /locus_tag="VG274_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWE05998.1"
                     /translation="KGFGSYGFPESHAASFAHLVYISAWIKRFHPAAFACALLNSQPM
                     GFYAAAEIVRDAREHDVETRHVDVNFSAWDNTLEHRAGGTLALRLGLRQIDGFREDWA
                     TTLIASRGNGYADVDGLARRTHLPKRALILLAEADSFQSLAVDRRESLWAVRRLADSD
                     SLPLFQTQYVEEHTAEEIAPLPLMPLGEHVLADYRMLRLSLRAHLMQFLRALFQGEGV
                     MSCAEISKAADGARARCAGIVLTRQMPGDAGVVFITLSDETSVANVVVWPRLVETFRR
                     AIMGGRLLLVDGKIQRSPEGVVHLVAERIFDRTEELDRISEDETEPPIRAPDSTPRHP
                     RNVRIVPRSRDFH"
BASE COUNT         1157 a         1991 c         2139 g         1244 t
ORIGIN      
        1 agtttatcct tcggccgcgc ttcgcgcgga ccggggggct cggcacctcc cccacgatgg
       61 gggaggaaag aggaagcgcg ctcatcggct cagccctccg tccaatgtcg gaagatcgag
      121 cgcggcgaag ccgaaatgcc ggagccagcg cagatcggat tcgaagaagg cacgcagatc
      181 cggcgcgcca tatttcagca tcgccatgcg gtcgatgccg aagccgaagg cgaatccctg
      241 ataacgttcg ggatcgatgc cgcacatttc gagcacgtgg ggatggacca tgcccgagcc
      301 gccgagttcg agccagtcgg tgccggtgcc gaatttgatc tgcccgcccg agcggtcgca
      361 gcgcatgtcc cattcgacgc tgggttcggt gaagggaaag taggatggcc gcgcgcgcaa
      421 ttcgatgcgc tcgacctcga agaaggcact gcagaattct tccacgaccc agcgcagatg
      481 gccgagatgg gtttcctcgt cgatgacgag cgcttccacc tggtgaaaca tcggcgtgtg
      541 cgtggcatcg gaatcgcggc gataggtgcg ccccggcacg atgacgcgga tcggcggttt
      601 ctgcgacagc atcacccgcg cctggaccgg gcttgtatgg gtgcgcaaca cgtgtcgcgc
      661 gccatccgtc tggcgtgcga catagaacgt gtcgtgcatc tgccgcgccg gatgatcggg
      721 tggaatattc agcttggtga aattgtaatc gtcgaactcg acatccgggc cttcggcgac
      781 cgcaaagccc agatcggcaa agatcgcggt gatctcgtcc tgcacctggc tgaccggatg
      841 aatggtgccg cgcggctcgg gccgcggcgg aagcgtcaca tcgatgcgct cgaaggccag
      901 acgcgcgtcg agctttgatc gtgcgagcac agtcttgcgt tcggcgatcg ccgccgcagc
      961 acgatcgcgc agtccgttaa acagcgggcc cttctcccgg cgctcctccg ggctcatcgc
     1021 gcccagcgat ttcagaagct cgctgatcga gcctttcttg ccgagcgccg acacccgcac
     1081 cgtctccagc gcggcttcgt cccccgcgtt gccgatcgcg tcgaggagtc gccgttcgag
     1141 ggagtcgatg tcgctcatgc gtgatgcacg cttgcgcttt ggggcagtga aggaatgcta
     1201 actgacgagg cgcagaacgg ctacgcccgc gcctgctccg ccagcgcctt gaaggcatcg
     1261 ggctcgcgca ccgccagatc ggccaggatt ttgcggtcga gttcgatgcc ggcgcgggcg
     1321 agtccgctga taaagcggct gtaggtcaga ccgttaagac ggcaggcggc gttgatgcgc
     1381 tggatccaga gcgcgcggaa attccgcttc ttctccttgc ggccgatata ggcgtgctgg
     1441 agcgagcggt cgaccgcggc attggccgtg gtgatgttgt tctttcgccg gccgcggaat
     1501 cctttggcct gcttcaggac gcgcttgcgg cgcagatgcg agggaacggc gcgaggggca
     1561 cgggacatgg tcgggcctcc ctttacgccg agtacggcat ccagcggcgg acgcgctggg
     1621 tttccgaggc cgacatcact gtcgtgcccc gaagttcgcg aatctgctcg ttggagcgct
     1681 tgatcatgcc gtggcgcttg cccgcctggc cgtgcttgag cttgcccttg gcggtgaatt
     1741 tgaagcgctt cttggcgccc gatttcgtct tcagtttggg cattttgcgg tctcctggga
     1801 tggatcaccg gctcaaggcc ggtcacggac gcgttcgagg ccgccacggc atgccctgta
     1861 cgccgggcgg cccgaaagag gcggggtctt agacggaaaa gaggccggat ggcaagccgc
     1921 agcccgcctt gttgcggcat atgacccagc ctagtttcgc agacgacgaa ggaacggccg
     1981 agatgaagat gttgaacgaa ttccgcacgt tcggcgcgcg cttgagcgtg cggacggcgg
     2041 ttctggtcgc agtcctggtc tttgtgtgcg cgtcgcccgg cgcggcccgg tctgccccgg
     2101 ctagcgacga tgtccaggga gtcgcgaatg gtttctacgc ggtctacggc acattccgtc
     2161 cctccgacgg cattcccgac gcgaaggggc gggcgaaata cgaacctttt atctcgcccg
     2221 cgctcgatcg cctgttgatg gaaggcgaag ctgccgaacg gcgcttcgtg aacgcgacga
     2281 agcacatgtc gcccccgctg atcgaaggcg atttgttcac gtcgaatttc gaaggcgcaa
     2341 cctcgtggag cgtcgggccc tgcgagaccg cagccgcaac ggcgcactgc aaggtcgccc
     2401 tcgggtatcg cggcggtccc ggggaagatt caaagccggt caactggaca gatactatat
     2461 atcttgtgcg gaacggcgcc ggctggcgcg tcgacgacat cgcctatggt gcgccctggg
     2521 cttttggaaa caagggccga atgacgcaga cgctccaaag cgcgatccgc gacggcaaca
     2581 acgcaacgca atgatggttg tcggcaaata gtgaaggatg caaccggaaa tgaacaggaa
     2641 cgagtttgcc gagagcagga gcgacgcgga gtggcgcgcc aggctcaccg acgatcagta
     2701 tcgcgtgttg cgcgagcatg ggaccgagcc cgcgggatcg agcccgctca acgcggagaa
     2761 gcgcactggc gtgttccgct gcgccggctg cgggatggag ctgttcgaat cggacacgaa
     2821 atacgaaagc ggcagcggct ggccgagctt caccgcgccg cgcgaccaag ccgtcggcac
     2881 atcgcgcgat gccagccatc tgatggagcg cacggaagtg cattgcgcgc gctgcggcgg
     2941 gcatctgggc cacgtcttcg aagacgggcc gaggcccacc ggccagcgct attgcatgaa
     3001 cggtgcggct ctgacattcg aacctgcgaa agagtagggc cgggcgctgc ctccgcggcg
     3061 cggaaggggc gcgttggccg atccgctgca gactgctgaa caaatcgcgc aggtcgcgat
     3121 cccgatcatc gtcgccatca cgctgcacga agcggcgcat ggctatgcgg cgcgccattt
     3181 cggcgacgac accgccgcgc gcgccggccg catctcgctc aatccgctgc gccacattga
     3241 tctgttcgga acgatcctgc tcccgggact tctgctgctc tcgcacgcgg gttttgtctt
     3301 cggctacgcc aaacctgtgc cggtgaattt cgcggcgctc cgcaatccca agcgcgacat
     3361 ggtgtgggtc gctgcggcgg ggccgggcat gaacgtcctg ctcgcgctcc tctccgggct
     3421 cgcgttgttc acgatgctgc aattcggcgg aacggcccat aagatggcgc tcgaattctt
     3481 ctggacctcg gtcgaaatca atctcgtgct ggcggtgttc aacctcctgc ccgtgccgcc
     3541 gctcgacggc gggcgcgtcg cggtagggct tctccctcag ccgctttcgc tggcactggc
     3601 gcagctcaac cgcttcggca tgttgctgct gatcgcgatt ttcattctgc tgccgctgat
     3661 cgggatcaat ctcttccact acctcgtgca gatcccggtc gagttcctcg aacgcccgct
     3721 actgtcgctc ctcggaattc cggcgcagta acggccgcga gtagccggca aattcccggc
     3781 ttttatcgaa ttacggaagt gcaaagcctg gaagccgcaa tgccttgcga tcaaaggtga
     3841 ccgttcttgc acagcccgcc ttgtcaccga gtgcgaggat aagggcatcg gagaaggcgc
     3901 cgcggccatc cttgagcgcg atcatggcgg tgaaaacctg ttgctcgctt tctacgacga
     3961 gaacggccgt ttgcagtatg cgctcgatgc atgcggcgac ctcttcgtcg gcgagatcat
     4021 aggcccgttc cagaacccaa gcggtctcgg ccatggagac gacgctgacg aagccggggt
     4081 tctcctcgct cagccgccgt tcgatgaggt cgacggcttt tggtgactgg acggggtcgt
     4141 cctgcgcgag atagcgaacc aggacgttgg tatcgagtcc gatcatcgcc gcctgcggcg
     4201 cgatgttgcg ccagccgcga ctgcttcatt catcgcttcg actgttacgg gccgtcgccg
     4261 ggccttgagg atcccgcgta gcgccgtagg gggaagcttt ggcaagagga ccacgcttcc
     4321 gtcgggatgg agaaaaaatt tgagtctgtc gccgggcttg agcccgagat gctcccggac
     4381 cgccttcggt atcgttgcct ggcctttgac ggtaattgcg gactccattt cggcctccat
     4441 tacatttcgt ggaatgtaat acaacccggg gatcacggtt tcaagccccg gcggtcgggt
     4501 tgccgcgaga tggcctgtga tggaaggcct tcctcagtag atatggtata gttgaccaat
     4561 taggtggtca ggagtctgcg atgcggagcg tgaaccttgc ggaggcgaag gcgcatctaa
     4621 gtgagttggt cgaacgtgcg gcggcgggag aggtggtgtg catcatgcgg cgcggcaagc
     4681 ctgtggcgaa gttgacgggc ctgggtacga agcgcaaacg cgtcgatgcg gcggcactgc
     4741 gcacattgac ggctccgatg ccgaaacaga agcagaccgc aggtgagctt gtgcggagga
     4801 tgcgcgacaa ggatcggtat tgatacacta tctcgacacg tctcttcttg ttgcggcatt
     4861 taccaatgag accagaacgc aagagatgca gagctggctc ggccggcaga cgccgggctc
     4921 gctcgcaata agcgaagggg tcgtaacgga gttttccgcg gcactgtcga tcaagctctg
     4981 gaccagtcag atcgccgccg cccatcgcgc tggcgctctg tcaatgttcg ggcggacggc
     5041 cgacgagagt ttcgtgttgc tgcccaaccc cgtggaatcg tcaccgagcc tttgatagtc
     5101 ggtgcggatt ccattttgtt ttacttgtct tcggctggtt gccggcgacg gccgcctcca
     5161 tctccttcag ggatttggga cgcccggcat aggccagcga accgaagaca tcttccggtc
     5221 gcgtgggcgc gaaaagcggt gcggccttca ggactacgcc gtcggaagtg ctttcgacgg
     5281 tgaggcgtgt gcctgcgtgc cagcggcgct tttgtttatg cttttgggta gagtgatctg
     5341 acctgcgttt gacacggtcg tagtgagttt tcgagtgttt gccatgcgtc gtgctcttga
     5401 gccgattcgg aacggatatg cttccaggaa gaccgctccg ccagtgcgga gataaagcgt
     5461 catgcgcagc gtccaattca agccgcctca atgaaaatcc cgcgaccgcg ggacgatgcg
     5521 gacgttgcgc ggatggcggg gcgtgctgtc gggcgcgcgg atgggtggtt cggtttcgtc
     5581 ttcggagatg cggtcgagtt cctccgtgcg gtcgaagatc cgctccgcca ccagatgcac
     5641 cacgccttcg ggactgcgct ggattttgcc gtcgacgagc aacaacctcc cgcccatgat
     5701 cgcgcgccgg aatgtttcca caagcctcgg ccagaccacc acattggcga cgctggtctc
     5761 gtcgctcagc gtgatgaaga cgacgcctgc atcgcccggc atctggcgcg tcagcacgat
     5821 gccggcgcaa cgtgcccgtg cgccgtccgc agctttcgaa atctccgcgc agctcatcac
     5881 gccttcgcct tgaaacagcg cacgcaggaa ttgcatcaga tgcgcccgga gcgacaggcg
     5941 cagcatgcga tagtcggcga gcacatgctc gcccagcggc atgagcggca gaggggcaat
     6001 ctcctccgcg gtgtgttcct ccacatattg cgtctggaag agaggaagag aatcgctgtc
     6061 cgcgaggcgc cgaaccgccc acaggctctc gcggcgatcg accgccaacg attgaaagct
     6121 gtcggcttcc gccagaagga tcagcgcccg tttgggcaga tgcgtgcgcc gcgcgaggcc
     6181 gtccacatcg gcataaccgt tgccgcgcga cgcgatcagc gtcgttgccc agtcttcgcg
     6241 gaagccgtcg atctgccgca atcccagccg cagcgcgagc gtgccgccgg cgcgatgttc
     6301 cagcgtgttg tcccaggcgc tgaaattcac atcgacgtgg cgcgtctcga cgtcgtgctc
     6361 gcgtgcgtcg cgcacgatct cggcagccgc atagaatccc atcggctggg aattgagcag
     6421 cgcgcaggcg aaagccgccg gatgaaatct cttgatccag gccgagatgt agacgagatg
     6481 cgcgaaactc gcggcatggc tctccggaaa gccgtagctg ccgaacccct t
//
LOCUS       DASZMC010000237         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3406776,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000237 DASZMC010000000
VERSION     DASZMC010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3406776"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VG274_04775"
     CDS             complement(<1..684)
                     /locus_tag="VG274_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWE05999.1"
                     /translation="MKSRWSDSEAQDFIARYASRHIGEDIALRVYTTRLLGSDPRLVL
                     HGGGNTSVKTQMQDLLGDRLDVICVKGSGWDMGNIEPAGLPAVKLASLRRLRALDRLS
                     DEDMVNYQRVNLLDASAPNPSVETLLHAFLPHKFVDHTHSTAVLALTDQPDGESIARD
                     VYGDRVAYVPYTIPGFALAKSVADIFDRHRDVEGLVLLQHGIFTVGKNARETYERMIE
                     LVTLAEERLK"
     gene            complement(681..1814)
                     /gene="mtnA"
                     /locus_tag="VG274_04780"
     CDS             complement(681..1814)
                     /gene="mtnA"
                     /locus_tag="VG274_04780"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HWE06000.1"
                     /translation="MASQLGFQNVKIAGRDYRTIWPVGDDNVEVIDQTRLPHEFATVR
                     LARAEDAARAITTMVVRGAPLIGATAAYGVALAMRADASDENLDRVHDLLFAARPTAV
                     NLRWALARMRAALRNQPRGERAGLAWREAAAICDEDVETCRRIGEHGLKILRDAAAKK
                     NGERLNLLTHCNAGWLACVDWGTALAPVYMAHDAGIPLHVWVDETRPRNQGASLTAFE
                     LGGHGVPHTIIADNAGGHYMQRGDVDLCIVGTDRVAANGDVANKIGTYLKALAAGDNN
                     VPFYVALPGSTIDWTIARGGDIPIEERSSDEVLKMTGRLEGGGLATVEIGAPGSAAAN
                     PGFDITPARLVSGFITERGTCAASRDGLLSLFPERRAETQDRK"
     gene            complement(1916..2443)
                     /locus_tag="VG274_04785"
     CDS             complement(1916..2443)
                     /locus_tag="VG274_04785"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWE06001.1"
                     /translation="MDFRSVIRSIPDYPKTGIIFRDITTLLGHPRAFRRAVDELVQPY
                     AGVRIDKVAGIEARGFILGGAVAHQLSVGFVPVRKKGKLPWQVMGEDYALEYGTDRVE
                     IHTDAVKHGDHILLVDDLIATGGTCFAAIKLLERAGGKVIGCSFVIDLPDLGGAEKIR
                     VLGKEVKTLVSFEGH"
     gene            complement(2449..3327)
                     /locus_tag="VG274_04790"
     CDS             complement(2449..3327)
                     /locus_tag="VG274_04790"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWE06002.1"
                     /translation="MTKTVLGVIGGSGVYDIAGLENARWERVGSPWGEPSDSLLAGRL
                     SGVDMLFLPRHGRGHVHSPTSINYRANIDALKRAGATDIISVSACGSLRETLPPGMFV
                     MADQFIDRTHARERSFFGRGFVAHVSLAHPVCPRLADALDEACAEEGIAHARGGIYLA
                     MEGPQFSTLAESNLYRSWGCDVIGMTAMPEARLAREAELPYAIAAMVTDFDCWHPDHD
                     HVTVDQVVAVLSQNAEKARRLIARVAPKLGPERTPSPLGIEHVLDTAFITAPEKCDPE
                     LFAKLDAVAGRMLRKG"
     gene            complement(3520..>4028)
                     /locus_tag="VG274_04795"
     CDS             complement(3520..>4028)
                     /locus_tag="VG274_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HWE06003.1"
                     /translation="AEPNDKGELFDSSGNRLTRPGIVADHFPPPFANEQAARAANGGA
                     LPPDLSLIEKARAGGAAYVYSIVTGFHEKPPAWFKVLPNKYYNPYFQDWNISMPPPLA
                     DGSVTYSDGTSSKVDQEAHDVVTFLAWASEPSMEERKRLGLGVMAFLIVFSGLLFLSY
                     RRVWRDAH"
BASE COUNT          735 a         1308 c         1268 g          717 t
ORIGIN      
        1 cttgagccgc tcttccgcga gcgtgaccag ttcgatcatg cgttcatagg tttcccgtgc
       61 gttctttccg accgtgaaga tgccgtgctg caggagaacg aggccctcga cgtcgcgatg
      121 gcggtcgaaa atgtcggcca cgctctttgc gagggcaaac ccgggaatcg tatagggcac
      181 ataggcaacg cggtcgccat agacgtcgcg cgcgatgctc tcgccgtcgg gctgatcggt
      241 cagggccagc acggcggtcg agtgggtgtg atcgacgaat ttgtgcggca ggaaggcgtg
      301 cagcaacgtc tcgacggacg gattgggcgc gctcgcgtcc agcagattga cgcgctgata
      361 attgaccatg tcctcgtcgg agaggcgatc gagggcgcgc aggcggcgca gggacgcgag
      421 cttcaccgcc ggcaaacccg cgggttcgat gtttcccatg tcccagcccg agcccttgac
      481 gcagatcaca tcgagcctgt cgccgagcag gtcctgcatt tgcgtcttca cggacgtatt
      541 gccgccgcca tgcagcacca gacgcggatc gctgcccaaa agccgcgtcg tgtaaacgcg
      601 cagcgcgata tcctcgccga tgtgccggga ggcgtagcgg gcgatgaaat cctgggcttc
      661 ggaatccgac cagcggcttt tcatttccgg tcctgcgttt ctgcgcggcg ttcgggaaaa
      721 agggagagaa gtccatcgcg cgaggcggcg caagttccgc gttcggtaat gaacccgctc
      781 acgagacgtg ccggcgtgat gtcgaagccg ggattcgccg ccgcgcttcc cggcgcgccg
      841 atttcgaccg tcgccagccc gcctccttcc agccgccccg tcattttcag cacctcgtcg
      901 ctggagcgtt cctcaatcgg aatatcgccg ccgcgtgcga tggtccaatc gatcgtagaa
      961 cccggcagcg ccacatagaa cggcacgttg ttgtcgccgg cggccagcgc cttcagatag
     1021 gtgccgatct tgttcgcgac gtcgccattg gcggcgacgc gatccgtgcc cacgatgcag
     1081 agatcgacat cgccccgctg catgtaatga ccgccggcat tgtccgcgat gatcgtgtgg
     1141 ggcacgccat ggccgcccaa ttcgaacgcc gtgagagagg cgccctggtt gcgcgggcga
     1201 gtttcgtcga cccacacatg cagcggaatc cccgcatcat gggccatata gaccggggca
     1261 agcgccgttc cccaatcgac gcaggccagc cagcccgcgt tgcaatgggt gagaaggttg
     1321 aggcgctccc cattcttctt cgccgcggcg tcacggagga tcttcagacc gtgctcgccg
     1381 atgcgccggc acgtctcgac gtcttcatcg cagatcgcgg cagcttcgcg ccacgccaga
     1441 cctgcgcgct cgccgcgcgg ttgattgcgc agcgcagccc gcatgcgcgc cagcgcccag
     1501 cggagattga ctgcggtcgg ccgggccgcg aacaggagat catgcacgcg gtcgagattc
     1561 tcgtccgagg cgtcggcgcg catcgccagc gcgacgccat aagcggccgt cgctccgatg
     1621 agcggcgccc cgcgcacgac catcgtcgtg atggcacgcg ccgcatcctc cgcccgcgcg
     1681 agccgcaccg tcgcaaactc gtgcggcagc ctcgtctgat cgatcacctc gacattatcg
     1741 tcgccgaccg gccagatcgt ccgatagtcg cgtcctgcga tcttcacgtt ctgaaatcca
     1801 agctgactcg ccaagaagat tcacagggag acagtgaggc agtgagaacg attgcgcgct
     1861 ttgcgcgcat tttttcctct caccgtctca ctgactccct gtgaatgttc ttttctcaat
     1921 gcccttcgaa cgaaacgagg gtcttcactt ccttgccgag gacgcggatc ttctccgcgc
     1981 cgccgaggtc ggggagatcg atgacgaagg agcagccgat gacctttccg cccgcccgct
     2041 cgagaagctt gatcgcggcg aagcaggtcc cccctgtcgc aatgagatcg tcgacaagca
     2101 ggatatggtc gccgtgcttc accgcgtcgg tgtgtatctc gaccctgtcg gttccgtatt
     2161 cgagcgcgta atcctcgccc atcacctgcc agggcagctt gcctttcttg cgcaccggca
     2221 cgaagccgac cgagagctga tgcgcgaccg cgccgcccag aatgaaccca cgcgcctcga
     2281 tacccgccac cttgtcgatg cgcacgccgg catagggttg cacgagttcg tccactgcac
     2341 gccggaatgc ccgcggatgc cccagcaatg ttgtgatgtc gcgaaagatg atgccggtct
     2401 tcggatagtc cggaatcgag cgaatgaccg atctgaaatc catgtgcgtc agcccttcct
     2461 gagcatccgg cccgccaccg cgtcgagctt ggcgaacaac tccggatcgc atttctccgg
     2521 cgccgttatg aacgcggtat cgagcacatg ctcgataccg agcggcgaag gcgtacgctc
     2581 cggcccaagc tttggcgcca cgcgtgcgat caggcggcgc gccttctcgg cattttgcga
     2641 cagcacggca acaacctgat ccaccgtgac gtgatcatga tcgggatgcc agcagtcgaa
     2701 atccgtcacc atcgccgcaa tcgcgtaggg aagctcggcc tcgcgcgcca gtctcgcctc
     2761 gggcatggct gtcatgccga tcacgtcaca gccccaggag cggtagagat tcgattcggc
     2821 aagcgtggag aactgtgggc cttccatggc gagatagatg ccgcccctgg cgtgcgcaat
     2881 gccttcctcg gcgcacgcct cgtcaagcgc gtcggcgagc ctggggcata cgggatgggc
     2941 gagagacaca tgcgcgacaa aaccgcggcc gaagaagctc ctttcgcggg catgggtgcg
     3001 gtcgatgaac tgatcggcca tcacgaacat gccgggtggc agagtctcgc gcagtgagcc
     3061 gcacgcggaa acggaaataa tgtcggtggc gccggcgcgc ttcagcgcat cgatattggc
     3121 gcgatagttg atcgaagtcg gcgaatggac atggccgcgg ccatggcgcg gcaggaacag
     3181 catgtccacg cctgagagcc ggcccgccag caaggagtcc gaaggctcgc cccagggact
     3241 tccgacccgt tcccagcgcg cgttctcaag gcccgcgatg tcgtagacgc cgctgccgcc
     3301 gatcacgcca agaacggttt tcgtcatccg ctgacgagct ctccacttgt catggcctgc
     3361 gcgtgaagct tggcctccgg ccgcgcttcg cgcggaccgc ggggcgggcc acccagacga
     3421 agccgctccg aagattcatt cagcacgcgg acgcgcgctg gctgggtgac ccggttaaac
     3481 cgggccatga caattaatag ctggctctga gcacctcgat caatgcgcgt cgcgccagac
     3541 gcggcgatag gagagaaaga gcaggccgga aaagacgatc aggaacgcca tcacgcccag
     3601 gccgagccgc ttgcgttcct ccatgctggg ctccgacgcc caggcgagga aggtgacgac
     3661 gtcatgcgct tcctgatcga ccttgctgga agtgccgtcg gaataggtga cggagccatc
     3721 ggcgagcggc ggcggcatgg aaatattcca gtcctggaaa tagggattgt agtatttgtt
     3781 cggcagcacc ttgaaccagg ccggcggctt ttcgtgaaag ccggtcacga tcgaatagac
     3841 ataggccgcc ccgcccgccc gcgccttttc gataagcgaa agatcgggcg ggagcgcgcc
     3901 gccgttcgca gctcgcgccg cctgctcatt tgcgaatggc ggcgggaaat gatcggccac
     3961 gatgccggga cgcgtcagac gatttccgct gctgtcgaag agctcgccct tgtcgttcgg
     4021 ctcggcgg
//
LOCUS       DASZMC010000238         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4229177,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000238 DASZMC010000000
VERSION     DASZMC010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4229177"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2266)
                     /locus_tag="VG274_04800"
     CDS             complement(<1..2266)
                     /locus_tag="VG274_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HWE06004.1"
                     /translation="MRKRPSSGRRCYRGLIFAATFVPVPAYASATRIAFEVAPLIAGG
                     ALALGLAAALWASALASVVRRLRRELREVGAFTRSAVGARDALIAASRDAVVAWGGDE
                     SLPRSYGAADRIVESCLAGPDAPLLAEALDALSDRGAPFLLSIRDERERPVTLRGRAV
                     GAMAAIWVEASPATGTTDFRAVLDAIPIPVWLRDPTRALAWGNRAWLAATGQPDTETA
                     IAAQASLEKSDRDLASAARSEGRALEAKRYAVVAGQRRTLALTYVPLADSSVAGSATD
                     VTDLSNAEARLQQHIDAHADTLDKLATAVAIFDRQRKLTFYNGAFVRLWNLPEAWLDI
                     HPTDEEILDRLRETRKLPEQRDYQAWKRQRLALYAKAGDYVPDDLWYLPGGKTLRVVA
                     QPHPFGGLTFLYEDVTEKLALESSYNTLIKVQSATLDTLSEAVAVFGPDAKLKLHNAA
                     FARLWELRESDVVGEPHVQKLAEACIAHFGDAAIWQNLAVSIASGAERSRNWGQFERS
                     DKTVLALAFAPLPDGATLITFANVTDRFRIESALRDRAEALEAADRLKSDFVHHASFL
                     FRDPLNAVHGFASLLVSGAAGALTSKQEEYVNDILAASDNLADVTSDILDLALIDSGA
                     MRIELARVDLHVLLSRVAETLRKHAESLDIEFRRDWDEHIGEIMADGRRLRQVVFNLL
                     SNAFKYTPREGTITLSAAIMGDDVQISVADSGPGIAPEVKANVFDRFSARGRGGQPAG
                     AGLGLALVNRFIELH"
BASE COUNT          414 a          777 c          814 g          396 t
ORIGIN      
        1 catgcagctc gatgaaacgg ttgacgagcg cgaggccaag ccctgcgccc gcgggctggc
       61 cgccgcggcc tcgggcggag aagcggtcga agacgttcgc cttgacctcc ggcgcgatcc
      121 ccggaccgga atcggcaacg cttatctgca cgtcgtcgcc catgattgca gccgaaaggg
      181 tgatcgtgcc ctcgcgcggc gtatatttga acgcgttcga cagcagattg aacacgacct
      241 gccgcaggcg ccgcccgtcc gccatgatct ctccgatgtg ctcgtcccaa tcgcggcgga
      301 actcgatgtc gagactctcc gcgtgcttgc gcagcgtttc ggctacgcgc gacagcagga
      361 cgtggagatc gacgcgcgcg agttcgatcc gcatcgcgcc cgaatcgatc agcgccaggt
      421 cgaggatgtc gctcgtcacg tcggcgagat tgtctgaggc ggcgagaata tcgttcacat
      481 attcttcttg tttcgatgtc agcgctccgg ctgcgccaga cacgagcagg ctggcaaaac
      541 cgtgcaccgc gttcagcggg tcgcggaaga gaaacgacgc gtgatgcacg aaatcggatt
      601 tcagccggtc ggccgcttcg agcgcctccg cgcggtcgcg caacgcgctt tcgatgcgga
      661 agcggtcggt gacgttggcg aatgtaatga gcgtcgcccc gtcgggaagc ggtgcgaaag
      721 caagcgcaag cacggttttg tcggagcgtt cgaattgccc ccagttgcgg ctgcgttcgg
      781 cgcccgaggc gatgctgacc gcgagattct gccagatcgc cgcgtcgccg aagtgcgcga
      841 tacaggcttc ggcgagtttc tgcacatgcg gctcgcccac gacatcggat tcccgaagct
      901 cccacagccg ggcgaaagcc gcgttgtgca gcttcagctt tgcgtcggga ccgaacacgg
      961 ccaccgcctc gctgagcgta tcgagggttg ccgactgcac cttgatgagg gtgttatagg
     1021 aactctcgag tgcgagcttc tcggtcacgt cctcgtagag gaatgtcagg ccgccgaaag
     1081 gatggggctg cgccacgacg cgcaacgtct tgccgccggg caggtaccac agatcgtccg
     1141 gcacgtagtc gcccgccttt gcatagagcg cgagtctctg gcgtttccac gcctggtagt
     1201 cgcgctgttc gggaagtttg cgcgtctcgc gcaggcggtc gagaatctct tcgtccgtgg
     1261 gatggatgtc gagccaggct tccggcagat tccacagccg cacgaacgca ccgttgtaga
     1321 aggtgagctt gcgctggcgg tcgaagatcg cgacggccgt ggcaagcttg tcgagcgtgt
     1381 cggcatgcgc gtcgatgtgc tgctgcagcc gcgcctcggc gttcgacaga tcggtcacgt
     1441 cggttgccga acccgccacg ctgctgtccg cgagcggcac ataggtgagc gcaagcgtgc
     1501 ggcgctgtcc ggccacgacc gcgtagcgct tggcttcgag cgcgcgtcct tccgaacgcg
     1561 cggcggatgc gagatcgcgg tccgattttt cgagcgaggc ctgcgctgcg atggcggttt
     1621 ccgtgtcggg ctgaccggtg gcggccagcc aggcccggtt gccccaggcg agcgcccgcg
     1681 tcggatccct gagccacacg ggaatcggaa tcgcatcgag gacggcgcgg aagtcggtcg
     1741 tccccgtcgc cgggctcgcc tcgacccaga tcgcggccat cgcgccgacc gcgcgcccgc
     1801 gcaatgtcac cgggcgttcc cgctcgtcgc ggatcgacag caggaatgga gcgccgcggt
     1861 cgctgagcgc gtccagcgcc tccgcgagca ggggcgcatc gggtccggcg aggcaggatt
     1921 cgacgatccg gtcggcggcg ccgtaactgc gcgggagcga ctcgtcaccg ccccaagcaa
     1981 ccaccgcgtc gcggctggca gcgatcagag cgtcgcgcgc gccgacggcg gagcgggtga
     2041 acgcgcccac ctcgcgcaac tcgcgccgga gacgccgaac cacgctcgct agagctgatg
     2101 cccagagagc ggcggcgaga ccgagcgcca atgcgccgcc ggcgatcagt ggcgccactt
     2161 cgaacgcgat gcgagttgcc gatgcgtaag ccggcacggg aacgaaagtg gccgcgaaga
     2221 tgaggcccct gtagcagcgc cttcccgaag acggccgttt ccgcactccg cccaatccca
     2281 agatgggagg cagcgtctgg caacctccca cgcagccccg caagggggcg aatcactttg
     2341 gaatcaatat gatatgcacg agccgcgagt ccgcataggc gatgtggtta aaaaatcgta
     2401 a
//
LOCUS       DASZMC010000239         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1218792,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000239 DASZMC010000000
VERSION     DASZMC010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218792"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VG274_04805"
     CDS             <1..382
                     /locus_tag="VG274_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWE06005.1"
                     /translation="DGDGARFILDGVARSLRVVRRGPELTVIIAGQNYLLIQEDPLAP
                     PPTETAGDERVTAPISGRVARVLALPGDVVEKNAPLVVIEAMKMELTLRAPRSGKIAK
                     VFHDVDEMVEEGTEIVTFMTEEGA"
     gene            379..1293
                     /locus_tag="VG274_04810"
     CDS             379..1293
                     /locus_tag="VG274_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004782490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HWE06006.1"
                     /translation="MTLASRVRIVEVGPRDGLQNEPNTVTLEAKVALIEALAGAGLKT
                     IEAGSFVSAKWVPQMADTAEVLARLDLESGIHYPVLVPNLQGLDRALSADVREVAVFA
                     SASESFSQRNINCSIEESFDRFAPVVEKARGHGLKVRGYVSCVLGCPYEGDVVPQTVA
                     KVASRLFALGCYEISLGDTIGTGTPIKARKLIERVGAQIPVERLALHFHDTYGQALAN
                     ILACIELGVSAIDSAVAGLGGCPYAEGASGNVATEDVVYMLRGMDVETGVDLDALAAA
                     GRTISRLLDREPASKVARALAGRAKGRQ"
     gene            1355..3049
                     /locus_tag="VG274_04815"
     CDS             1355..3049
                     /locus_tag="VG274_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWE06007.1"
                     /translation="MNEIAESYVSGVADVPLLGQTIGAALERAATQWADRPALISPTQ
                     QIHWTWRELRDRADALAAGLLALGLRRGDRVGIWSLNRAEWTLTQFAAARAGFILVTI
                     NPAYRLSELEFALNKVGCSALVTATAFKSSDYLGMLKTLAPELDVSKPGELSAARLPH
                     LRFVMQIGGPQVPGTIAFDEVAAQGGASQFAELRASTRERQFDDAINIQFTSGTTGSP
                     KGVTLTHHNILNNGYFVGRAMRLTAEDRICIPVPLYHCFGMVMGNLAAVTHGCAMVYP
                     GEGFDPLATLEAVQTQNCTALYGVPTMFIAELDHPEFARFDLGSLRTGIMAGAPCPIE
                     VMRRVVDRMNLREVTIAYGMTETSPVSFQSAVDDPLERRVSTVGQIQPHLEAKIVDSD
                     GRIVPRGQSGELCTRGYSVMLGYWDDREKTNEVIEEAGWMHTGDLATLDADGYCNIVG
                     RIKDMVIRGGENLYPREIEEFLFRHPQIQEVQVFGVADDKYGEELCAWVKLRDGETMT
                     EEEMREFCRGQIAHQKVPRYVEFVDSFPMTVTGKAQKFLMRKNVEDRLGLKAVETA"
BASE COUNT          647 a          960 c          982 g          597 t
ORIGIN      
        1 tgatggcgac ggggcgcggt ttatactcga tggcgtggcc cggagcttgc gggtcgttcg
       61 ccgcgggccc gagctgacgg tgatcattgc tggacagaac tatctcctga tccaggaaga
      121 tccactcgcg cctccgccga cggagactgc tggcgatgaa cgcgtcactg ctccgatctc
      181 gggccgtgtt gcgcgggtgt tggctctgcc aggcgacgtc gtcgagaaga acgcgccctt
      241 ggttgtgatc gaggcaatga agatggaatt gacgttgcgc gctccgcgga gcggaaaaat
      301 cgcgaaggtt ttccacgacg tagatgagat ggtcgaagag ggaaccgaga tcgtcacgtt
      361 tatgaccgag gagggcgcat gactctcgct tcaagagtgc gcatagttga agtcggaccg
      421 cgcgacgggc tccagaacga gcccaacacc gtcacgctcg aagcgaaagt ggcgctgatc
      481 gaggcccttg ccggggcggg acttaagaca atcgaggctg gcagcttcgt ctctgcaaaa
      541 tgggtcccgc agatggccga caccgcggaa gtgttggcgc gcctcgatct cgagtccggc
      601 atccactacc ccgtccttgt cccgaatctt caagggctcg atcgcgcgct cagtgcggac
      661 gtacgcgagg ttgcggtgtt cgcctcggcc tccgagagct tctctcaacg caatatcaat
      721 tgttcgatcg aagaaagttt cgaccgcttc gcgccggttg ttgagaaggc gcgcggccac
      781 ggtcttaagg tgcgcggcta tgtctcatgc gtgctcggct gcccttacga aggcgacgtg
      841 gttccgcaga ccgtggccaa ggtcgcctct cggctcttcg cgttgggctg ttacgagatt
      901 tctctcggcg acacaatcgg aaccggtacg ccgattaaag cgcgaaaatt gatcgagcgc
      961 gtcggagcgc agattcctgt cgagcgtctc gcactgcact tccacgatac ctatggccag
     1021 gccctggcca atatcctcgc ctgcatcgaa cttggcgttt ccgccattga cagcgcggtc
     1081 gcgggtctgg gcggatgtcc ttacgccgag ggcgcaagcg gcaatgtcgc aacagaggac
     1141 gtcgtttata tgctgcgcgg catggacgtc gagaccggcg tagatctcga cgcgctcgcg
     1201 gcggcgggcc gaaccatatc gcggctgctc gatcgcgaac ccgcctcgaa ggtcgcgcgt
     1261 gcgctcgccg ggcgggcgaa aggccgccag tgaggcaagc gtcaggcact tcatccgaaa
     1321 ggccgtcgat gcagccgatc gcaaggaggt ttgcatgaac gaaatcgcag aaagctacgt
     1381 ttccggagtg gcggatgtac cgcttctcgg gcaaacgatc ggcgccgcgc tcgaacgcgc
     1441 cgcgacgcaa tgggccgatc ggcccgcctt gatctcccct acgcaacaga tccactggac
     1501 ctggcgcgag ttgagggatc gcgccgacgc cctagccgcc ggactcctgg cgctagggct
     1561 gcgccgcggc gaccgcgtcg gaatctggtc tctcaatcgc gccgaatgga cgttgacgca
     1621 gttcgccgcc gcgcgcgcgg gcttcattct cgtgacaatc aatccagcgt accggctgtc
     1681 cgagctcgaa tttgcgctta acaaggtagg ttgctccgcc ctggtgacgg cgaccgcttt
     1741 caagagcagc gactatctcg gcatgttgaa aacattggcg cccgagctcg acgtctcgaa
     1801 gcccggcgaa ctgtccgccg cgcgcttgcc ccacctacgc ttcgtcatgc agatcggcgg
     1861 cccgcaagtt cccggaacaa ttgccttcga cgaagttgcc gcgcagggcg gtgcatcgca
     1921 gttcgccgaa ttacgcgcct caacgcgcga gcgtcagttc gacgacgcga tcaacattca
     1981 gttcaccagc ggcaccaccg gttccccgaa gggagttacg ctgacgcatc acaacatcct
     2041 caataacggc tatttcgtcg gccgcgccat gcgactgacc gcggaagacc gcatctgcat
     2101 tcccgtgccg ctctatcatt gcttcggcat ggtgatgggc aacctcgccg ccgtgaccca
     2161 tggctgcgcc atggtctatc ctggcgaagg gttcgatccg ctggcgacgc tggaggcggt
     2221 gcagacgcag aattgcaccg cgctctacgg cgtgccaacg atgtttatcg ccgagctcga
     2281 tcatccggaa tttgcgcgtt tcgacctcgg ttctttgcgc accggaatca tggcgggcgc
     2341 accctgtccg atcgaagtaa tgcggcgggt ggtcgaccgc atgaatctgc gcgaagtcac
     2401 tatcgcctat ggcatgacgg aaacgagccc ggtgagtttc cagagcgccg tcgacgatcc
     2461 gcttgagcgc cgagtctcaa ccgtaggaca aattcaaccg caccttgaag cgaagatcgt
     2521 cgatagcgac ggccgcatcg ttccgcgcgg ccagtccggc gaactctgca cccgcggcta
     2581 ctccgtcatg ctgggctatt gggacgatcg ggaaaagacc aacgaggtca tcgaggaagc
     2641 gggctggatg cacacgggcg acctcgccac cctcgacgcg gacgggtatt gcaacatcgt
     2701 cggacgaatc aaagacatgg taattcgggg tggagaaaat ctttatccgc gcgaaatcga
     2761 ggagttcctt ttccgtcatc cgcaaattca ggaggtccag gtgttcggag tcgccgacga
     2821 caaatacggc gaagaactct gcgcctgggt caaattgcgg gacggcgaga cgatgacgga
     2881 ggaggaaatg cgagaattct gccgtgggca gatcgctcac caaaaagttc cacgctacgt
     2941 cgaattcgtc gattccttcc cgatgaccgt cactggcaaa gcgcaaaaat tcctaatgcg
     3001 caagaacgtc gaagatcgtc tcgggctgaa ggcggttgag accgcctaaa cccgcaacaa
     3061 attcagaacc tcaagtatcc ggatacgaaa tgaaggcgaa gtcaggctca ctcgcaatgg
     3121 acgcaagctt cgacaaacgg cagcgtcgca gtctggcgtg catttagaaa ctgcgcgaaa
     3181 agccca
//
LOCUS       DASZMC010000240         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_660067,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000240 DASZMC010000000
VERSION     DASZMC010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660067"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VG274_04820"
     CDS             complement(<1..494)
                     /locus_tag="VG274_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HWE06008.1"
                     /translation="MRHNTGLQEEQILAHDAPQSGKNTMSPAEETAMEQQTAATPRAS
                     GLRLYPGYLDRASQMALVAALERIFAVAPLFTPRMPKSGRPMSVRMSNCGPLGWITDE
                     AGYRYEPNHPVTKQPWPPMPEVLIAMWNELGGYRCLPQACLINFYGATAKMGLHQDRD
                     EEDF"
     gene            895..1203
                     /locus_tag="VG274_04825"
     CDS             895..1203
                     /locus_tag="VG274_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06009.1"
                     /translation="MRSSIVKNYGRRLINEYADDLLAAVAFWRQVGAWRSKLRKKVSP
                     APAPSTPADPIASDNRNVIALAEALAERRRASVVQTILHSDEKSESEDSLDRPMTAAR
                     "
     gene            complement(1263..>2666)
                     /locus_tag="VG274_04830"
     CDS             complement(1263..>2666)
                     /locus_tag="VG274_04830"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWE06010.1"
                     /translation="VYVGGISRTVDYKGFLFDIGGHRFFSKSKEVVALWQELLPDDFI
                     TRPRLSRIYYNGKFFSYPLKAFEALLKLGVFTSAACLASYARAKLRPLAEPRTFHQWV
                     RNQFGERLFQIFFKTYTEKVWGMSCDEISADWAAQRIKGLDLATAVVNALTRSLRLRR
                     KAGPVGDTVKTLIESFQYPRKGPGMMWQAAAHKIRALGGRIMMGRELATLGYDADRQL
                     WHIEIAVAGGGRETYTARHVVSSAPLRELVERIKPTPISLLHARALRYRDFLTVALMV
                     RKGETFPDNWIYIHDPSVKVGRVQNFRSWSPEMVPEGMSCLGLEYFCFEGDGVWNAPD
                     SELIALAKTEIAQIGLATPDDVVDACVVRQAKAYPVYDEDYADNVAIIRRDLEGRYPT
                     LHMVGRNGMHRYNNQDHAMMTAMLTARNIIAGARLYDIWNVNEDAEYHEAGESGVQDA
                     LESVRRVPEKVAPDRAA"
BASE COUNT          484 a          786 c          852 g          544 t
ORIGIN      
        1 tcgaaatcct cctcgtcgcg atcctggtgc agtcccatct ttgccgtcgc gccatagaaa
       61 ttgatcagac acgcctgcgg caggcagcgg taaccgccga gctcgttcca catcgcaatc
      121 agcacctccg gcatcggcgg ccaaggctgt ttggtgaccg gatggttcgg ctcgtaccga
      181 tatcccgcct catcggtgat ccagccgagc ggaccgcaat tggacatgcg cactgacatc
      241 ggccggcccg atttcggcat gcggggagta aacaagggtg ccaccgcaaa tattcgctcc
      301 agtgcggcga ccaaggccat ctgcgaggcg cggtcaagat agcccggata gagccgcagg
      361 ccgctcgcgc gcggagttgc ggcggtttgc tgctccatcg ccgtttcttc tgccgggctc
      421 atcgtgtttt taccactttg cggggcatcg tgcgctaata tctgctcttc ctgcaggccg
      481 gtattgtggc gcaatccggt cgttcggaca aaacgccgac cgagctttgt gggccaaact
      541 ggcaagcgtc tcggcgcttt cctcgcgctg atccaggtct cctatttaat ttatgcggcg
      601 gtaatgccaa cccgatcact gggtgctcat caggagtcgt agctcgcttg tacggcacgc
      661 ctttctgggc tgcgcgatca gcaaagtctg tggatagcgc gcgaacatgg cttgtttgca
      721 gtgcgcggct caggcgcaat gaccgtggaa gagtggtgtg gggtggcgtc catggttgcg
      781 gagagttccg gtgcgcgacg caccagggtt cgggagtgcg tctgttgcgg agcggctacc
      841 gcaacgccaa gccttccagc atgctgggat gattggaatt tgctccccga ggatttgcgc
      901 tcgtcaatcg tcaaaaacta cgggcgccgt ctgatcaacg aatacgctga cgatctgctg
      961 gccgccgtgg ctttttggcg ccaagtcggc gcgtggcgca gcaagctccg taagaaggtt
     1021 tctcctgcac ccgcgccaag cactcccgcc gatccgattg cgagcgacaa ccgcaatgtc
     1081 attgcacttg cggaagccct tgcggaacgg cgacgagcct ccgtggtgca gacgattctg
     1141 cattcggatg agaagtcgga gtcggaggat tcgctggacc ggccgatgac cgcagcgcga
     1201 tagagtaccg cagcttgcac tttgctgccg atgatcgagc gggctggcga ttcgcgcaag
     1261 agctaggcgg cccgatccgg ggcgactttt tcgggaacgc gccgcacgct ttcgagcgcg
     1321 tcctggacac cggattctcc ggcctcgtga tattcggcgt cctcgttgac gttccagatg
     1381 tcgtaaagcc gcgcgccggc gatgatgttg cgtgcggtca gcatcgccgt catcatcgca
     1441 tggtcctgat tgttgtagcg gtgcatgccg ttacggccga ccatgtggag tgtcggatat
     1501 ctgccttcga ggtcgcgccg gatgatggcg acgttgtcgg cgtaatcttc gtcgtagacc
     1561 ggataggcct tggcctgacg caccacgcag gcatccacca catcgtcggg tgtagcaagc
     1621 ccgatctgtg cgatttccgt cttggcgagc gcaatgagtt cactgtccgg cgcgttccac
     1681 acgccatcgc cttcgaagca gaaatattca aggccgaggc agctcatgcc ctcgggcacc
     1741 atttcgggcg accaggaacg gaaattctgg acgcggccga ccttgacgct cggatcgtgg
     1801 atgtaaatcc agttgtcggg aaatgtttcg cccttgcgca ccatcagcgc aactgtgagg
     1861 aaatcacggt agcgcagcgc gcgtgcgtgc agcagcgaaa ttggggtcgg cttgattcgc
     1921 tcgaccagtt cgcgcagcgg cgccgagctg acgacgtggc gcgccgtgta ggtttcgcgc
     1981 ccgccgcctg caacggcgat ttcgatgtgc cagagctgtc tgtcggcgtc gtagccgagc
     2041 gtcgccagtt cgcgacccat catgatgcgg ccgccgagcg cgcggatctt gtgcgccgcc
     2101 gcctgccaca tcatgccggg gcccttgcgc ggatattgga agctttcgat cagcgtcttg
     2161 acggtgtcgc cgacagggcc ggctttgcgg cgcagccgca gcgagcgcgt cagcgcgttg
     2221 acgacggcgg tggcgagatc gaggcccttg atgcgttgcg ccgcccaatc ggcggaaatc
     2281 tcatcgcacg acatgcccca gactttctcg gtgtaggttt tgaagaagat ctggaacaga
     2341 cgctcgccga attgattgcg cacccactga tggaacgtgc gcggctccgc caggggtctg
     2401 agcttggcgc gggcgtagga ggcaaggcac gcagcgctgg tgaagacgcc gagcttgagg
     2461 agagcttcga acgccttgag cgggtaggag aagaatttgc cgttgtagta gatgcgagac
     2521 agccgcggcc tggtaataaa atcgtcaggc aacagctcct gccagagcgc aacgacttcc
     2581 ttcgatttgg agaagaagcg atggccgccg atatcgaaca gaaagccttt gtagtcgacc
     2641 gtgcggctga tgccgcccac atagac
//
LOCUS       DASZMC010000241        12834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2299072,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000241 DASZMC010000000
VERSION     DASZMC010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2299072"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2360)
                     /locus_tag="VG274_04835"
     CDS             complement(<1..2360)
                     /locus_tag="VG274_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HWE06011.1"
                     /translation="MSGGVDEQRIAASASEQGEHRTTIVGVGASAGGIKALQEFFEEL
                     PAEPGVAFVVIVHLDPKSRSELPEILAARCKMPAQQVTKTSRLKPNCIYVIPPDRQLK
                     ISDDEIAAIPFQEPRGQRAPIDLFFRSLAQQHGDAYAIILTGAGSDGAVGVKAIKESG
                     GIVLVQDPEEAEYPSMPLASIATEAADFVLPIRPLANRLLELLRARRQTSSGLRDSSE
                     EEILRRILSHLRVRTGHDFSHYKRSTVVRRIVRRTQVARRDTLEQYFAYLRENPDEAQ
                     LLLTDLLISVTTFFRDARSFEKLAKLVIPRLFDAKDATIPIRIWVVGCATGEEAYSIG
                     MLMLEEAARHDLRPDIQIFATDLDTRALAIAREGRYPATIETDVSEDRLRRFFQRDGD
                     HFRVKRELRDMVLFASHSLLKDPPFSHLDLVSCRNLLIYLNRELQEQVVSTLHYALSP
                     TGFLFLGSSESADHTEHLFQPVDRESRLYQSRGLSDRKPPLPPGLLGVTHIGERISPE
                     ASGGARGLRAVHRDALESLAPPSAVVDNNCRVVHLSESAGRFLHISGGVLSSEITDLV
                     REEMRQDLRAALHRAFEHDESTLSPAMLVQFNGTPHRVYIQVRPMPGRENDSHARRAL
                     VLFIEGEGGTHAALPDAVPGPPKSDTVRRLQEELELAHLHLRTKRDESEAATEELRAA
                     NEELQSINEEYRSTAEELETSKEELQSINEELQTVNSELKLKLDTVSRSNSDLQNLMA
                     ATDFATLFLDPQLVIKRFTPRTTDLFNLTANDIGRPITDFTHRLKYA"
     gene            complement(2628..3386)
                     /locus_tag="VG274_04840"
     CDS             complement(2628..3386)
                     /locus_tag="VG274_04840"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HWE06012.1"
                     /translation="MHEMPDLLLIGDGRLARHLARYFAQLGIRHAAWSRRMYEEGGCP
                     ALATLVRPRTHVLLAISDSAIGSFVCGHPELEPTVRVHFSGGLATSLAIGAHPLFSFA
                     NALYERELYERIPFVIDEGAPRLSALIPGLPNPSFFIRPDQRARYHALCVLAGNFTTL
                     LWRKLFFELDREYGIPREQALPYLESVARGLAETGAPLSGPLSRGDAATIARNLEALK
                     GDPFAQVYRAFVEAYEQQQELAAAGTEKNWLAPH"
     gene            3893..6661
                     /locus_tag="VG274_04845"
     CDS             3893..6661
                     /locus_tag="VG274_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWE06013.1"
                     /translation="MILARAVAATIAIIVACLIVLGLASSFLVDWAWFSSVGYLAVFW
                     TMISVKSALFSAVFLASAAAIWLSGTLAVRLAAPRASRRPANMPWESLGSEQLLAVIE
                     RFMRRLPWRPAVAIAAIVLATLVALGWTDNWNLALNYIFQAPYGRSDPLFGNDFSFYL
                     FALPLFVALKDWLLVVLVLSALLAGIVYWACGEIAFNARRRFVSAAAAAHGSVLLGFF
                     FAIEAWSFDLDRYLLLYDDNGVVVGASYTDIHVRLPILTALTALCCAAAIASFANVRL
                     RSARIPIVSAALILGTSLVLSPLAAALFQRAYVKPNELQLEQPYIGRNIALTREAYNL
                     RNIVVKPFTADQSLTSQSLEDNRATIDNIRLWDRQPLLDTYAQLQEIRTYYKFNDAGI
                     DRYVLGGAYQQVMLSARELESTLLPPNAQTWVNLHVLFTHGNGAVMSPVTRTSPEGLP
                     IFYLRDIPPVAAGGPPVAEPRIYFGEGDAPYVIVKGSTPEFDYPKGNDNVYRAYDGAD
                     GVSIGGIARRALFAWYFGDPNILLSQYVTAESRVMFRRNIQERVSAIAPFLGLDRDPY
                     LVVSGGRLYWIQDAYTTSSWFPYAQHMEGDPTNYIRNSVKIVIDAYNGSVDFYVADPT
                     DPIVETWRRIFPTLFKPFAAMPADLQNHTRYPEDLFLIQAQIYRAYHMNRPEVFYNRE
                     DLWQFPRQPNGNEDAADSKMSPYYINMRLPGETRTEFVLMLPMAPSQRENMIAWLAAR
                     CDRPDYGKLIVYEFPKDKLVYGPFQIEALINQNTEVSQQITLWNQNGSRVIRGNLLVV
                     PIGNSILYVTPLYLRAQSGQLPELKRVIAVYGDRVVMEETLAGALAALFKPTPTIPAA
                     RTPAASSAAGVAVGPPAERAREALAHYDRAIAKLKAGDWSGFGAELNALGPLLEQLNQ
                     PASGQPQR"
     gene            complement(6768..7979)
                     /locus_tag="VG274_04850"
     CDS             complement(6768..7979)
                     /locus_tag="VG274_04850"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWE06014.1"
                     /translation="MPLIRHAKPVEDDFMRVPDGAEISAQGPVLLQAAQFMTRAGELR
                     GSRVGVLWPNDRNVAELVPHLGQLALVALDFPSFRDGRAYSQARLLRERHFYRGELRA
                     TGDVLSDQLVFMLRAGFDSFELRKDTDVTRFHEAVGRYRDFYQPASDARDPIARLRLK
                     VCRPQGEGIQLPRPDAAALEALLTNAAPHEIVGRAIRAVRRGKLAVVSSFGIESAALL
                     KIVSDVDRSLPVIFLDTGWLFQDTLDYRDALIAHLDLSDVRTIRPAPAAIEKSDADYS
                     LWARDSVACCNLRKVAPLAAALAPFDAWITGRKRYEGGQRALLPIVEEDGPRLKFNPL
                     ARVSPKTVAAIFRTLPRHPLAAAGYTSIGCVPCSSKAREQEGVRGGRWRETGRTECGI
                     HTEALASATAM"
     gene            complement(7966..9621)
                     /locus_tag="VG274_04855"
     CDS             complement(7966..9621)
                     /locus_tag="VG274_04855"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWE06015.1"
                     /translation="MYFYDDFDRTFLTERVAEFRGQVARRLSGDLTEDEFKPLRLMNG
                     VYLQLHAYMLRIGIPYGTLSSDQLRAIADVARRHDRGYGHFTTRQNIQFNWIRLEELP
                     DAMAELARAGLHGIQTSGNVVRNITTDQWAGVAPGEVEDPRIWAEILRQHTTLHPEFS
                     FLPRKFKIAITASDEDRAAVRVHDIGLRLHRNDAGEAGFEVMVGGGLGRAPFLARTIR
                     PFLAKRDFLTYIEAILRVYNQYGRRDNIYKARIKILVHELGAEVFARDVEAEWLAIKD
                     GPLALDPSIVEEIASRFRYPDYAHVETAPTRDAVFAGDPAFGMWRANSVARHKVPGYS
                     TVTLSLKPEGGPPGDATADQMDAIADLADRYSYGEIRVGHDQNLVLPHVAEGDLRDLW
                     RALDELGVATPNIGLASDIISCPGLDYCSLANTRSIPVAQELTRRFRDLARTRDTGRL
                     HINIWGCINACGHHHVGHIGLLGVEKNGEEFFQVTLGGRSDAKAALGTLVGPAIPYAE
                     VADVVEGIVDVYLGLRAGPEELFVDAVKRVGLEPFKERIYAAH"
     gene            complement(9608..9949)
                     /locus_tag="VG274_04860"
     CDS             complement(9608..9949)
                     /locus_tag="VG274_04860"
                     /inference="COORDINATES: protein motif:HMM:NF022464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HWE06016.1"
                     /translation="MTAPQQQKLKVSGPVIVTANRLGDGAVVYRTSDNSWSANLQSAR
                     VVTTAADATNLLREALSDEQRVVGPYVASVRIDSSAGLSPANLRERIRSEGPTIALPT
                     ARERPRRHVLL"
     gene            complement(9946..11346)
                     /gene="cysG"
                     /locus_tag="VG274_04865"
     CDS             complement(9946..11346)
                     /gene="cysG"
                     /locus_tag="VG274_04865"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HWE06017.1"
                     /translation="MRYLPVVLDLNRGFVAVVGATPPALGKLRILRDLGASVRWFPCD
                     GAATIRPVPDGGGPLEIIEGEPAAQDIANVIAVISAAGGGTDERIARIAREFRTPINV
                     VDRQDLSSFIFPAIVDRGDVVVAISTGGAAPMLARRLRAQIETLLPKRIGQLAGFLGR
                     WRAKFKQRGGENLASGRVFWEKIVDGPIAVRVLEGREREADDLMSALGKTGVESKGSV
                     AIVGAGPGDPDLLTLKALHALQNADIIFYDDLVTPEILARSRREAVKVFVGKRKGRPG
                     QTQSEINARLVATARAGNRVVRLKGGDPFIFGRGGEEIEALREAGVAFTVVPGITAAL
                     GCAAEAELPLTFRNEAASLVFVTAHRSQDAPPIDWASAGGADATLVVYMGQSCAAKVR
                     DGVIAAGRDPATPACVLARGTRAHARAQVGRLDELPLLAARAGDGPAILVIGNAVAHS
                     RPWSDLALQSDIPAVA"
     gene            complement(11736..12188)
                     /locus_tag="VG274_04870"
     CDS             complement(11736..12188)
                     /locus_tag="VG274_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWE06018.1"
                     /translation="MDVIDRRIVAALQEDASLSFAEVGKRVGLSPTPCWKRIQRLEED
                     GVILRRVAVIDPDKVGLGTTVFVSIETGEHSKEWLEHFAEIVCAMPEVMEFYRMAGDV
                     DYMLRVVTTDIHGYDAFYKRLIEAVPLKNVTSRFAMERIKSTTVLPLT"
     gene            12298..12549
                     /locus_tag="VG274_04875"
     CDS             12298..12549
                     /locus_tag="VG274_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWE06019.1"
                     /translation="MTIASACGVDSPDPKHRILKSVFGFDVFRPGQETVIDALLEGRH
                     VLTVMPTGSGKSLCFQVPALLRRLPERSSTLARSRANGN"
BASE COUNT         2362 a         4070 c         4056 g         2346 t
ORIGIN      
        1 gcgtatttca gccggtgcgt gaaatcggtg atcgggcgcc cgatgtcgtt tgcggtgagg
       61 ttgaagagat cggtggtgcg cggcgtgaag cgcttgatga cgagctgcgg atcgaggaac
      121 aatgtggcga aatccgtcgc cgccatcagg ttctggagat cgctgttcga gcgcgagacg
      181 gtgtcgagct tgagcttcag ttcgctgttg acggtttgca gctcttcgtt gatggactgc
      241 aattcctcct tgctggtttc gagctcctcc gcggttgagc ggtattcctc gttgatggac
      301 tgcagttcct cgttggcggc gcgcaactcc tccgtcgcgg cttccgattc gtcgcgcttg
      361 gtccgaaggt ggagatgggc cagctccagt tcctcctgca ggcggcgaac cgtgtcgctt
      421 ttcggcggac cgggaacggc atccggcagg gctgcgtgtg ttcctccttc accttcgatg
      481 aaaagaacca gcgcgcgcct ggcatgggaa tcattctccc gcccgggcat cggcctgacc
      541 tgaatataga cgcgatgcgg cgtgccattg aattgcacga gcatggccgg gcttagcgtg
      601 ctttcgtcgt gctcgaacgc gcgatgcagc gccgcgcgca aatcctgtct catttcttcc
      661 cgcacgagat cggttatttc ggagctcagc acgccccccg aaatatgcag aaagcggccg
      721 gcactttcgg agagatggac gaccctgcaa ttgttatcca cgacggcgct tggaggggcc
      781 agcgattcca gggcgtcgcg atgcaccgcg cggaggccgc gcgcaccgcc agatgcctcg
      841 ggcgaaatcc gctcgccgat atgggtcacg cccagaaggc cgggaggaag aggcggcttt
      901 ctgtcggaga gtcctctcga ctggtaaaga cgcgattccc gatcgacggg ctgaaacaga
      961 tgttccgtgt gatcggcgct ctccgacgac ccgaggaaga gaaatcccgt cgggctgagt
     1021 gcataatgca gcgtcgagac aacctgctcc tgcaattccc ggttcagata gatgagaaga
     1081 ttgcggcagg aaacgaggtc cagatgggag aacggcgggt ccttgagcag gctgtggctg
     1141 gcgaaaagga ccatgtcgcg cagttcgcgc ttgacgcgga aatgatcgcc gtcgcgctgg
     1201 aagaaccggc gaagacggtc ctcgctcaca tcggtctcga tggttgcggg gtagcggccc
     1261 tcgcgcgcga tcgcgagcgc gcgggtgtcc aggtcggtcg cgaaaatctg tatgtcgggg
     1321 cgaagatcgt ggcgcgccgc ctcctcgagc atcagcattc caatgctgta cgcttcttcc
     1381 ccggtagcgc agcccaccac ccatatgcgg atcgggatcg ttgcgtcctt ggcgtcgaaa
     1441 agcctgggga tcacgagctt tgcgagtttt tcgaacgatc gggcgtcgcg gaagaaggtt
     1501 gtcacggaaa tgagcaggtc cgtgagcagc aactgggctt cgtccggatt ttcccgcaga
     1561 taggcgaagt actgctcgag cgtgtcgcgg cgcgccacct gcgtgcgccg gacgatccgc
     1621 cgcaccaccg tggaccgctt gtaatgcgag aagtcgtgtc ccgtgcggac gcgcaggtga
     1681 gagagaatgc ggcgaagaat ctcctcctcg ctgctgtcgc gcaacccgga gcttgtctgg
     1741 cgccgcgcac gaagcagttc cagaagacgg ttggcgagcg gccggatcgg caggacgaaa
     1801 tccgccgcct cggtcgcaat cgacgcgagc ggcatcgagg gatattccgc ctcttccgga
     1861 tcctggacga ggacgatgcc gcctgactcc tttatcgcct tgacgccgac ggcgccgtcg
     1921 gagcccgcgc cggtcaggat gatcgcgtat gcgtcgccgt gctgttgcgc gagcgagcgg
     1981 aagaaaagat cgatcggcgc gcgctgtccg cgcggctcct gaaacggtat ggcggcaatc
     2041 tcatcgtcgg aaatcttcag ctgacgatcc ggcggaatga cgtagatgca attgggtttc
     2101 agccgcgacg ttttcgtaac ttgctgcgcc ggcattttgc agcgggcagc gagtatctcg
     2161 gggagttcgc tgcggctctt gggatccaga tggacgatga cgacgaaggc cacgcccggt
     2221 tcggccggca gttcctcgaa aaattcctgg agcgctttga tgccgcccgc cgatgctccg
     2281 acgccgacga tcgtggtgcg gtgctcgccc tgttcggacg cgctggccgc gatacgttgt
     2341 tcgtccaccc cacccgacat ctggcgatgc cttccttgat ccaccgacag tagcagcgtc
     2401 cgttccctgc taccagcggc ggaattgcct tgcgcctctt atagggaacg cctggctggc
     2461 ggctgtcggg tgcgaaacct gcccgaattt ctcccgccgc gtcggcagcg caaggtccaa
     2521 aatcgtgacg gccaaacatt tgatgggaaa ttgcctcaat ttcctgggag ggcggttcag
     2581 gcgcagtgcg gcctttcccc atcccggttg aacatcgttg ttggctgtca gtgcggcgcg
     2641 agccagtttt tttccgtgcc cgccgctgcg agttcctgtt gctgttcata cgcttcgacg
     2701 aaagcgcggt agacctgcgc gaacggatct cccttcagag cttcgagatt gcgcgcgatg
     2761 gtcgccgcat cgccgcggct cagcggaccc gaaagcggcg cgcccgtttc cgcgagaccg
     2821 cgcgccacgg attcgagata gggcagtgcc tgttcgcgcg ggatgccata ttcgcgatcg
     2881 agctcgaaaa acagctttct ccacagaagc gtcgtgaagt ttcccgcgag cacgcaaagc
     2941 gcatggtagc gtgcgcgctg gtctggcctg atgaaaaagg acggattcgg cagccccgga
     3001 atcagcgcgg aaagccgggg agcgccctca tcgatgacga agggaatccg ctcgtagagt
     3061 tcgcgctcgt aaagcgcatt ggcgaagctg aagaggggat gtgcgccgat ggcgagcgaa
     3121 gtcgccaggc cgccggagaa atggacgcga accgtcggct ccagctcggg atgtccgcaa
     3181 acgaaagacc cgatcgcgct gtcgctgatc gccagaagca catgcgtccg cgggcggacg
     3241 agcgtggcca gcgcgggaca tcccccttcc tcgtacatgc ggcgcgacca ggcggcatgg
     3301 cggatgccaa gctgcgcgaa gtagcgcgcg aggtggcggg caagccggcc gtcgccgatc
     3361 agaagcaggt caggcatctc gtgcatggat tcgatctcgc cccgtcatgc tgatcgtaat
     3421 cgacatcctt tatgtcaacc acctggaaaa agagagcgtg gacacgtaga gccgggaccc
     3481 tggcacctcc ttttgatctt tagtgacttg tgtgtcggca gatttttaac gccgagtacg
     3541 cggaggatgc gcggagggcg cagggtaagg atagcgcgcc tgcggcgcgc caactctttt
     3601 ctgcgcccac cgcgcatcct ccgcgacctc tgcgttaaat ctgtcttagt atcgctcgtc
     3661 ataaattccg gcaatttgcc cgctcgacat gacgcgccca gccgccaaat tgcgacacca
     3721 cctgtgaatt tgccgcggcg gcgggcgcga aagcgccgga cggggcaggt ttgccgaggt
     3781 ggaaggtatt cggccgatcg cgccgcgcac ccatgacctc acccgcgccg gctttcgctg
     3841 tcgggccggg gaccaacgcg ctattgttca tgcggatgca ggggatgcgt gcatgattct
     3901 ggccagagcc gttgcggcaa caatcgcgat catcgtcgcg tgcctcatcg tgctggggct
     3961 cgcgagcagc ttcctcgtcg actgggcgtg gttctcctcc gttggctatc tcgcggtgtt
     4021 ctggacgatg atcagcgtga aaagcgctct gttcagcgcc gtcttcctgg ccagcgccgc
     4081 ggctatctgg ctgagcggaa cgcttgcggt gcgcctggcg gcgccgcgcg cctcccggcg
     4141 tccggcgaac atgccctggg aatcgctggg cagcgagcag ttgctggcgg tgatcgagcg
     4201 cttcatgcgc cgcctgccct ggcgcccggc cgtcgcaatt gcagccatcg tcctggcgac
     4261 gctggtcgcg ctcggctgga ccgacaactg gaacctggcg ctcaactata tcttccaggc
     4321 gccgtatgga cgcagcgacc cgctcttcgg caatgatttc agcttctatc tgttcgcgct
     4381 tcccctgttc gtcgcgctga aggactggct tctggtcgtg ctcgtgttga gcgcgcttct
     4441 cgccggcatc gtctattggg cctgcggcga gatcgcgttc aatgcgcgcc gccgcttcgt
     4501 ctcggctgcc gccgccgcgc acggctccgt cctgctcggg ttcttcttcg ccatcgaagc
     4561 atggtcgttc gatctcgacc gctacctgct tctttatgac gacaacggag tcgtcgtggg
     4621 cgcgagctat accgacatcc acgtccgctt gccgatcctg acggcgctca ccgcgctctg
     4681 ctgcgctgcc gccatcgcct cgttcgcgaa tgtgcggctg cgctcggcca ggataccgat
     4741 cgtgtccgcc gcgctgatcc tcggaacgtc actggtactt tcgccactgg ccgcggcgct
     4801 gtttcagcgc gcctatgtga aacccaacga gctgcagctc gagcaaccct atatcgggcg
     4861 caacatcgcg ctgacgcgcg aggcctacaa tctcaggaac atcgtcgtca aacccttcac
     4921 cgcggaccag tcgctcacca gccagtcgct ggaggacaat cgtgcgacga tcgacaacat
     4981 caggctgtgg gaccggcaac cgctgctcga cacctacgcc cagctccagg aaatccgcac
     5041 ctactacaaa ttcaacgatg cgggtatcga tcgctatgtg ctcggcggcg cgtatcagca
     5101 ggtgatgctg tcggcgcgcg aactggaatc cacgctcctt ccgcccaacg cccagacctg
     5161 ggtcaacctc cacgtgctgt tcacgcatgg caacggcgcc gtgatgtcgc cggtgacgcg
     5221 cacctcgccg gaaggtctgc cgatcttcta tctgcgggac attccgccgg tcgccgccgg
     5281 cgggccgccg gtcgccgagc cgcgaatcta tttcggcgag ggcgacgcgc cttacgtgat
     5341 cgtcaaaggc agcacgccgg agttcgacta tccgaaggga aacgacaatg tctaccgcgc
     5401 ttacgatggc gcggacggcg tctccatcgg cggcatcgca cggcgcgctc tgttcgcctg
     5461 gtatttcggc gatccgaaca ttctgctcag ccagtacgtc acggccgaaa gccgggtcat
     5521 gttccgccgc aacatccagg agcgcgtgag cgccatcgca cccttcctcg gcctcgatcg
     5581 cgatccctac ctcgtcgtca gcggcggccg gctctactgg atccaggatg cctacaccac
     5641 gagcagctgg tttccctacg cccagcacat ggagggcgat ccgacgaact acatcagaaa
     5701 ttccgtcaag atcgtcatcg atgcctacaa tggcagcgtg gatttctatg tagccgatcc
     5761 caccgatccg atcgtcgaga cctggcggcg catatttccg acgctgttca agccgtttgc
     5821 ggcgatgcct gccgatctgc agaaccacac ccgctatccc gaggatctct ttctcatcca
     5881 ggcgcagatc tatcgcgcct atcacatgaa caggcccgag gttttctaca accgcgaaga
     5941 cctttggcag tttccccgtc agccaaacgg caacgaggac gcggccgaca gcaaaatgtc
     6001 gccgtattac atcaacatgc gcctgcccgg cgagacacgc accgaattcg tcctcatgct
     6061 tcccatggcg ccgagccagc gcgagaacat gattgcctgg ctcgcagcgc gctgcgatcg
     6121 gcccgactac ggcaagctga tcgtctatga gtttccgaag gacaagctcg tctacgggcc
     6181 gttccagatc gaagcgctga tcaatcagaa taccgaggtg tcgcagcaga tcacgctgtg
     6241 gaatcaaaac ggctcacgcg tcattcgcgg caatctgctc gtcgtcccca tcgggaattc
     6301 gattctttac gtcacgccgc tgtatctgcg cgcccagtcc ggccagctgc ccgaactcaa
     6361 gcgcgtgatc gcggtttacg gcgaccgcgt ggtgatggaa gaaaccctcg ccggcgcgct
     6421 ggctgcgctc ttcaagccga cccctacgat ccccgctgcg cgcacgcccg cagcaagttc
     6481 cgccgcaggc gttgcggtcg ggcccccggc ggagcgtgca cgcgaggcgc tcgcccatta
     6541 cgaccgcgcg atcgccaaat tgaaggcggg agactggagc ggcttcggcg ccgagctgaa
     6601 tgcgctcggc ccgctgctgg aacagttgaa ccaacccgcc agcggccaac cgcagcggtg
     6661 acggcttcgt caccatgagt gccaagcgtt tcatgcggct cgcttgcgcg cctttgcggg
     6721 ccggacaagc tgaaaggatt cgcccgtcgc agcgtgccgc aggaaggtta cattgcggta
     6781 gctgaagcca gggcttccgt atgaataccg cattccgtgc ggcctgtttc tcgccagcgg
     6841 ccgccgcgga cgccttcctg ttcccgcgcc ttcgacgaac acggcacgca tccgatggag
     6901 gtgtatcccg ctgccgccag aggatggcgc gggagagtgc ggaagatcgc tgcgacggtt
     6961 ttcggcgaga cgcgcgccag cggattgaat ttgaggcgcg ggccgtcctc ctccacgatc
     7021 ggcaacaagg cccgctggcc gccttcatag cgcttgcgcc cggtgatcca cgcatcgaac
     7081 ggagcgaggg ccgcagccag gggtgcaacc ttgcgcaggt tgcagcaggc cacggaatcg
     7141 cgcgcccaca ggctgtagtc cgcatcggac ttttcgatcg cagccggcgc cggcctgatc
     7201 gtgcgcacgt cggacaagtc gagatgtgcg atcagtgcgt cgcgataatc gagcgtgtcc
     7261 tggaaaagcc agccggtatc gagaaagatg acaggcaggg acctgtcgac gtcagagacg
     7321 attttcagga gcgcggccga ttcaattccg aacgaggaaa ccaccgccag ttttccgcgc
     7381 cgaacggcgc gaatggcccg cccgacgatc tcgtgcggcg cggcgttcgt cagcaacgct
     7441 tcgagcgccg cggcgtcggg cctcggtaac tggattccct cgccctgcgg ccggcagacc
     7501 ttcaggcgca accgcgcgat gggatcgcgc gcatcgcttg cgggttgata gaagtcgcgg
     7561 tagcggccga cggcttcgtg gaagcgcgta acgtcggtgt ccttgcgcaa ctcaaaggaa
     7621 tcgaaccccg cgcgaagcat gaatacaagc tggtcgctca gcacgtcgcc ggtggcgcgc
     7681 aattcgccgc gatagaagtg gcgctcgcgc aacagcctcg cctggctgta ggcccttccg
     7741 tcgcgaaagc tgggaaagtc gagagcgacg agcgcaagct ggccgaggtg ggggacaagc
     7801 tccgccacat tgcgatcgtt cggccataac acgccgacgc ggctacctct caactcgccg
     7861 gcgcgcgtca tgaactgcgc cgcctgcagc agaaccggac cctgcgcaga tatttccgca
     7921 ccatccggaa ctcgcatgaa atcgtcttca acgggtttcg cgtgcctaat gagcggcata
     7981 gatgcgctcc ttgaaaggtt cgagaccgac gcgcttgacg gcatcgacga agagttcctc
     8041 ggggcctgcg cgcaggccga gatagacatc gacgatgcct tcgaccacgt ctgccacttc
     8101 ggcataggga atcgccgggc ccacgagcgt ccccagcgcg gctttcgcgt ccgaacggcc
     8161 gccgagcgtg acctggaaga actcctcgcc attcttctcc accccgagca atccgatatg
     8221 gccgacatgg tggtgaccac aggcattgat gcagccccag atgttgatat gcagacggcc
     8281 ggtgtcgcgc gtccgcgcaa gatcgcgaaa ccggcgcgtc agctcctgcg caaccgggat
     8341 cgagcgggta ttggcgagac tgcaataatc gagaccggga catgagatga tgtcggaagc
     8401 gaggccgata ttcggcgtcg cgacgccaag ctcgtcgagc gcgcgccaga gatcgcgcag
     8461 atcgccctca gccacatgcg gcaggacgag attctggtcg tggccaacgc ggatttcgcc
     8521 gtaactatag cggtccgcca ggtcggcgat cgcgtccatc tggtccgcgg tggcatctcc
     8581 gggcggtccg ccttccggct tgagcgacag cgtgacggtg gaatagcccg gcaccttgtg
     8641 ccgcgcgacg gaattggcgc gccacatccc aaatgcgggg tccccggcaa acacagcatc
     8701 ccgtgtcggc gccgtttcga cgtgcgcata gtcgggatag cggaagcggg aggcaatttc
     8761 ctcgacgatc gacggatcga gcgccagcgg gccatccttg atcgcgagcc actcggcctc
     8821 gacatcgcgc gcaaacacct cggcgcccag ttcgtgcacc aggatcttga tgcgcgcctt
     8881 gtagatattg tcgcgccgtc catactggtt gtagacgcgc aggatggctt cgatataggt
     8941 gaggaaatcg cgcttggcca aaaacggcct gatcgtcctg gcgaggaagg gcgcgcgacc
     9001 caagccgccg cccaccatca cttcaaatcc cgcttcgccc gcatcgttgc gatgcagtct
     9061 caggccgatg tcgtgaaccc gcaccgccgc gcgatcctca tcggaggcgg tgatcgcgat
     9121 cttgaacttg cgcggcagga acgagaattc gggatgaagg gtcgtgtgct ggcgcaggat
     9181 ttcggcccag atgcgcgggt cttcgacctc gcccggcgca acgccggccc attgatcggt
     9241 ggtgatgttg cgcaccacat tgccactcgt ctggatgccg tgcaggccgg cgcgcgcgag
     9301 ctcggccatg gcgtcgggca attcctcgag cctgatccag ttgaactgga tgttctggcg
     9361 cgtggtgaaa tggccatagc cgcggtcatg gcgccgcgcg acatcggcaa tcgcgcgcaa
     9421 ttggtcggac gacagcgtgc cgtagggaat gccgatgcgg agcatgtagg cgtgaagctg
     9481 gagatagacg ccgttcatga gccgcaacgg cttgaactcg tcttccgtca aatcgcccga
     9541 aagccgccgc gcgacctgac cgcgaaactc cgccacgcgc tcggtcagaa acgtgcggtc
     9601 gaaatcgtca tagaagtaca tggcgacgag gcctttcccg cgcagtggga agcgcgatcg
     9661 tcggaccctc gctgcggatg cgctcgcgca ggttggccgg cgaaagaccc gcgctcgaat
     9721 caatccgcac ggatgcgacg taggggccga caacgcgctg ttcgtcggat agcgcttcgc
     9781 gaagcagatt cgttgcgtcc gccgctgtgg tcacaacccg ggcagattga agatttgcac
     9841 tccagctgtt gtcgctcgta cgatagacga cggcgccgtc gccaagccgg ttcgccgtca
     9901 cgatcaccgg accgctaacc ttcagcttct gttgctgggg cgccgtcatg ccaccgccgg
     9961 aatgtcggat tgcagcgcca ggtcgctcca tggccgggaa tgggcgaccg cgttgccgat
    10021 caccagaatg gcggggccgt cacccgcacg cgcggcgaga agcggcaatt cgtcgagcct
    10081 tccaacctgc gcccgcgcgt gcgcgcgtgt gccgcgcgcc agcacgcagg ccggcgtggc
    10141 gggatcgcgt ccggccgcga tgacaccatc gcgcaccttc gcggcgcagc tttgccccat
    10201 atagacgacg agcgtcgcat ccgccccgcc ggcgctcgcc cagtcgatgg gcggcgcgtc
    10261 ttgcgaacga tgggcggtga cgaacacgag gctcgcggct tcgttgcgga acgtgagagg
    10321 aagttcggct tcggcggcgc agccaagcgc ggccgtgatg ccaggtacga cggtgaacgc
    10381 aacgccggct tcgcgtaaag cttcgatttc ctcgccgccg cggccgaaga tgaacggatc
    10441 gccgcccttc agccgcacca cgcgattgcc ggcgcgcgcc gtggcgacaa ggcgcgcgtt
    10501 gatctcgctt tgggtctggc ccggccggcc tttgcgcttg ccgacaaaga ccttcacggc
    10561 ctcgcggcgc gaacgcgcca ggatttcggg cgtgaccaga tcgtcgtaga aaatgatgtc
    10621 cgcattctgc agcgcgtgca gcgctttcag cgtgagcagg tccggatcgc ccgggcctgc
    10681 gccgacaatg gcgaccgagc ccttcgattc gacgccggtt ttccccagcg ccgacatcag
    10741 atcgtctgcc tccctctcgc ggccttcaag aacccggaca gcaatcggac catcgacgat
    10801 cttttcccag aacacacggc cggatgcgag attctcaccg cccctttgtt tgaacttcgc
    10861 tcgccagcgc ccgagaaaac ccgcaagctg cccgatgcgc ttcggcagca gcgtttcgat
    10921 ctgcgcccgc agccgccgtg ccagcatggg cgccgcgcct ccggtgctga tggcaaccac
    10981 gacatcgccg cggtcgacaa tggcgggaaa gatgaaggag gaaaggtcct ggcgatccac
    11041 gacgttgatc ggcgtgcgga attcacgcgc aatccgggcg atacgctcgt cggtcccgcc
    11101 gcccgcggcc gaaatgacgg caatgacgtt cgcaatatcc tgcgcggcgg gctcgccttc
    11161 gatgatttcg agagggccgc cgccatccgg cacagggcga atggttgctg caccgtcaca
    11221 aggaaaccag cgaacgctgg cgccaagatc gcgcaggatc cgcaacttgc cgagcgcggg
    11281 cggagttgcg cccacgaccg cgacgaaacc gcggttcaag tccagaacga ccggcagata
    11341 tcgcatgaaa ccctcggcaa tccgttgcgg agggtcattg ttataagcac gattttcgcg
    11401 ccgtaaagaa gaagaaattt ctgctgtttt gcggatccgt gatggaagtt cgtgctgcgg
    11461 gcgaacacgc aggcagaacg tccatcacgc cgaaaaccct gccccgctca cggcgcgcgc
    11521 aaaatcgcgt tccgtcgtca aaacacgatg atctggacgc tcccttttcc tcccccaacg
    11581 tggaggaggt gccgagcccc ccggtccgcg cgaagcgcgg ccagaggata aactccgcga
    11641 ggcggagggg ggcgtcgtac gcaaccaacc acgtccccct ccggcccttc gggccacctc
    11701 ccccacgttg ggggaggaac gaagcagcac ggagttcaag ttagcggaag aacggtggtg
    11761 gatttgatgc gctccatggc gaagcgcgac gtgacgttct tgagcggcac cgcctcgatc
    11821 agccgtttgt agaacgcgtc atagccatgg atatcggtcg tcacgacgcg gagcatatag
    11881 tcgacgtcgc cggccatcct gtagaactcc atgacctcag gcatggcgca gacgatctcg
    11941 gcgaaatgtt cgagccactc cttcgaatgt tccccggttt cgatggaaac gaaaaccgtg
    12001 gtgcccagtc cgaccttgtc gggatcgatc accgcgacgc gacggaggat gacgccgtct
    12061 tcctcgaggc gctggatgcg tttccagcac ggcgtgggcg aaaggccgac gcgcttgccg
    12121 acttcggcga aggagaggga ggcatcctcc tgcagggcgg cgacgatgcg ccggtcgatg
    12181 acatccatga ttgtgcttgt ccggttgtga cgcgctcggg aactcatccg aatagcaaaa
    12241 ttctttgaat ccaggcaaag gtcgaaaggc tgtaaaaata tcgccgcttg acaagcgatg
    12301 acgatcgcat ccgcatgtgg ggtggattcg cccgacccca aacaccgcat tctgaaatcc
    12361 gtcttcggct tcgacgtgtt tcgtcccggc caggagacgg tgatcgacgc gttgctggaa
    12421 ggccggcacg tgctcaccgt catgccgacg ggatcgggga aatcgctctg cttccaggtg
    12481 ccggcgctcc tgcggcgtct ccctgaacga agttcaacgc tggctcggtc acgcgcgaat
    12541 ggaaactaac gcgatctacg ccggagcgct gggcgaagag aaacgcacct tggcttggcg
    12601 tgggcggaaa tcgctggaag caactatccg cacactatga agcgcaattg aaggtccaga
    12661 aatgcgacga tcgtcttcgt cgcgctcaca ggaagcacat taatcgcaaa cgccagaatt
    12721 cagattgggg cggggacgtt cgacgacgcc ggccggctta ggatgccttc gcgcgacgcc
    12781 gaacgacaat ctcgtgggcc agatcgacga aggcgcgcaa gctcgcggcc atgt
//
LOCUS       DASZMC010000242         8366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3658908,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000242 DASZMC010000000
VERSION     DASZMC010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3658908"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..176)
                     /locus_tag="VG274_04880"
     CDS             complement(15..176)
                     /locus_tag="VG274_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06020.1"
                     /translation="MRPESGVNDSRHSADAPVAFGTTADGLVALGLGYPSVAITAPVV
                     VWSSTTGPL"
     gene            371..1582
                     /gene="coaBC"
                     /locus_tag="VG274_04885"
     CDS             371..1582
                     /gene="coaBC"
                     /locus_tag="VG274_04885"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWE06021.1"
                     /translation="MSLRGKSVLLIIGGGIAAYKSLELVRRLRERGVRVRAILTRAGA
                     EFVTALSVSSLTGEKTWQDLFSLNDEAEMGHIQLSRAADLLVVAPATADLMAKLAQGH
                     ANDLASTALLATDKPILLAPAMNVRMWNHAATQRNLARLTADGVISIGPNEGEMACGE
                     FGPGRMAEPEEIVEAIETLLARRVTLAGRKALVTAGPTREPLDPVRYLANHSSGKQGY
                     AIAAALAEAGADTTLVSGPVDLAPPPDVTLLKVETACEMLDACELVLPVDVAVCAAAV
                     ADWRPVSRAEGKLKKLDDDAAPLIRLAANPDILATISRSGKRPALVIGFAAETENVVA
                     NGEEKRRRKGCDWILANDVSAESGVMGGDSNRVHLISAAGVEDWPLMDKREVGRRLAQ
                     RIGAALKGAAE"
     gene            1579..2034
                     /gene="dut"
                     /locus_tag="VG274_04890"
     CDS             1579..2034
                     /gene="dut"
                     /locus_tag="VG274_04890"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWE06022.1"
                     /translation="MKIEILRLPHARDLALPAYATNGAAGLDLLAAVDTEIELRPGAR
                     IAVPCGIALALPESVEAQVRPRSGLALHHGVTVLNAPGTIDSDYRGEVMAILINLGEN
                     PFRIARGTKIAQLIISLHARAELVECSELPPSARGASGFGSTGSGRTLL"
     gene            complement(2305..3231)
                     /locus_tag="VG274_04895"
     CDS             complement(2305..3231)
                     /locus_tag="VG274_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWE06023.1"
                     /translation="MPALRIAHRGGAWLRPENTLCAFANAIALGADGAELDVQLTRDG
                     DVVVFHDFRLSPDLCRDADGKWLVPPTPRIVDLTAGEFALYDVGRARPESAYAREHPA
                     MIACDGARIPALAEVVALARRAPKLFRLFIELKTSLADPTPSASPERLAEATLAVLRN
                     SGYVDRSIIVGFDWRGLRRAKSVEPAVECWYSTRPQSWFRDGEPPPQDDPPPQPALQM
                     LRHWACTGTSPWAAGFDAVNHGSSILEAITAAGGDGWFPYAADATADAIAQAHRLGLK
                     VGAWTVDDPAQTRTLDAAGIDAICTDRPDLML"
     gene            3366..4616
                     /locus_tag="VG274_04900"
     CDS             3366..4616
                     /locus_tag="VG274_04900"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HWE06024.1"
                     /translation="MRLAREAAIAAFFLLAANAAAGAATAPPAPKKVGPDVLFEQGLF
                     DAAEAGYQAVSKTSPGYEGALRQLGAIALYRNHPQEAELRLRNALAHNPADARCAGLL
                     AETLNREGKFAEMAQLLRQIGRPERAAEFELFARIQPYRLRGRAAPVTIPFEQTNPLP
                     AVRAKVNDLEGLFLIDTGGAEILLDPEFAREAHVATTANAQGPSSAGRPAAPSFGRIA
                     QFALPGLQTDDVPAMLLSTKAFSSMARGKKVAGIIGTEYLSQFRATLDYVRDRLILEP
                     REAPMRSGARTAEIPFWMVGDHFLLAQGRLDRGPKQLLLVDTGFGRLAFTAPESTLRD
                     AGITMPAPQAGPSGRPPIAKFPIAQLSLGSLEEKNISGIYGAFPPGLENGLGVHVGGI
                     VSHEYFLPYAVTFDFVRMKIGIRK"
     gene            4976..6382
                     /locus_tag="VG274_04905"
     CDS             4976..6382
                     /locus_tag="VG274_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWE06025.1"
                     /translation="MSDIAEFDLAHAGRPVTGRGLDAWIAEARELAKLAAPLVLTQLA
                     QMAILTTDVVMLGRLGKTALASAALGNTVFFFTWLIGCGPVAAVAPMVAHILGARPGN
                     RAGVRNAVRMGFWAMLLLSAPLIVFLLFTQPILLALGQRPELAAGAGRFTGMLCWGLP
                     FSLGYQVLRNFSTALSRPNASLWAMGLGIFFNAVGDYALIFGHFGLPRLGLVGAGIAS
                     ACSYAFAFFMMLAVVRLMPALHKFRIFRRFHRLHWDKLVEVFRLGMPIGLTMIFEAML
                     FNAATLIMGTFGTASVAAHQIALNVPSITFMVPLGIGMAATVRVGLAAGAGDGDAVRR
                     AGYSALLMGAGFMSMTAIVLWMFPSAIANLYFADDAANADVIVLAVVFLRVAAAFQVC
                     DGLQVVGALSLRGLKDAHMPMWIAGLSYWLGGFPVCLWLAFGWHLKGLGIWIGLATGL
                     FVAAALMCWRFWFLSKAR"
     gene            6576..6992
                     /locus_tag="VG274_04910"
     CDS             6576..6992
                     /locus_tag="VG274_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06026.1"
                     /translation="MRKVAGVFAAIALGALLPLWTANAQLPAAGAPLGSKSETPGAFP
                     DVIPGDNILIACDAVEKRAADSDVRVVLTISAMPGESTTGYAKVLATDELLLKDAVRV
                     RIPLIPNLEQHTVGLDVYVVGSANAEHCDAGHMKIS"
     gene            complement(7030..7524)
                     /locus_tag="VG274_04915"
     CDS             complement(7030..7524)
                     /locus_tag="VG274_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06027.1"
                     /translation="MARAKQRGRYTGEESGNMGEEFEMKNEESEGAMPTGQTRQSLLD
                     AFDAFGGPMARVMEHNRTMLQKMMHAVQEESLKFVNRRLEHTGHAIESSRDCQGVSGL
                     LAIQQEWMLDFARDYAEHTRRFAELMGELVEDGSTSLTAASLSAIKETRARTEAANGR
                     RTGA"
     gene            complement(7656..>8366)
                     /locus_tag="VG274_04920"
     CDS             complement(7656..>8366)
                     /locus_tag="VG274_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWE06028.1"
                     /translation="FFHADMHQGNLFVDAESRLVCVDFGIMGRLDEPMRRFMAETLGG
                     FLARDYERVANVHYDAGFVPRTHPAPLFAQALRAIGEPVFGRRASCVSMAKLLQQLFD
                     TTRRFDMELQPQLVLLQKTMVVVEGVARALDPEFDIWEASRPVIEEWMIERIGPEARL
                     RDAAEGVSALGRLARDFPHLLRNADIISTMLADGGLKLHPDSVRQIAQAQVARTRHVR
                     VALWIAAGALAILAIGVL"
BASE COUNT         1445 a         2688 c         2675 g         1558 t
ORIGIN      
        1 cactacatcg gctcctacag cggccccgtc gtcgacgacc agaccaccac cggcgcggtg
       61 atcgcgacgc tggggtatcc gagccccagc gcgacgagcc cgtcggctgt cgttccgaac
      121 gcaacaggcg cgtctgccga gtgcctgcta tcgttgacac ctgattcggg cctcatcctt
      181 cgacaagctc aggatgaggc cctcatggtg agcctgtcga accatgaggg ccggcggggc
      241 ttaagacgct tctccgaatc cgggaccagg cgtgaaattc atgcttgctg gccccgggtt
      301 cttcaaaagc tacacttcgc aggcatcgtt cgctatatgc tgcagcgagc caagggagtt
      361 ctcgttaccc atgtcgttgc gcggaaaatc ggtcctgctc atcatcggcg gcggcatcgc
      421 ggcctacaag tcgctcgagc tggtgcgccg gttgcgcgag cgcggcgtgc gcgtgcgcgc
      481 gatcctcacc agggccggcg cggagttcgt gaccgccctc tccgtgtcgt cgctcacggg
      541 cgaaaaaacc tggcaggatt tgttcagcct gaacgacgaa gcggagatgg ggcatattca
      601 actgtcccgc gcggccgatc tgcttgtcgt cgcgcccgcg accgccgatc tgatggcgaa
      661 actggcgcag ggacacgcga acgatcttgc atcgaccgcg ttgctcgcga ccgacaaacc
      721 gatcctgctg gcgcccgcga tgaatgtgcg gatgtggaac cacgcggcga cgcaacgcaa
      781 cctcgcccgg ctgaccgccg acggcgtgat ctccatcgga cccaacgagg gcgagatggc
      841 ctgcggcgaa ttcggccccg gccgcatggc ggagccggag gagatcgtcg aggcgatcga
      901 gacattgctg gcgcggagag tcacgctggc ggggcgcaag gcgctggtga cggcgggtcc
      961 gacgcgcgaa cctctcgatc ccgtgcgcta cctcgcgaac cattcttccg gcaagcaggg
     1021 ttacgcgatc gccgcggcgc tcgccgaagc cggcgccgat actacgcttg tttccgggcc
     1081 ggtcgacctc gcgccgccgc ccgatgtgac gctgctgaaa gtcgagacgg cctgcgaaat
     1141 gctcgatgcg tgcgagttgg tcctgccagt cgatgtcgcc gtctgtgcgg ccgctgtggc
     1201 cgattggcgg ccggtctcgc gggcggaggg caaactcaag aaactcgacg acgatgccgc
     1261 gccgctgatc cggctggcgg ccaatcccga catcctcgcc acgatctcgc gctccgggaa
     1321 gcgccctgcc ctcgtcatcg ggttcgcggc cgaaacggaa aatgtcgtgg caaatggcga
     1381 agagaagcgc cggcgcaagg gatgcgactg gattctcgcc aatgacgtga gcgcggaaag
     1441 cggcgtcatg ggcggcgaca gcaatcgcgt gcatctgatc tccgccgccg gcgtcgagga
     1501 ctggccgctc atggacaaga gggaagtcgg tcgccgcctc gcccagcgca tcggcgcggc
     1561 gctcaagggc gccgccgaat gaagatcgag attctgcgtc ttcctcacgc gcgcgatctt
     1621 gccctgccag cttacgcaac gaacggtgca gcaggcctcg atctcctcgc cgccgtggat
     1681 accgaaatcg agcttcggcc gggcgcgcgc attgccgtcc cctgcggcat cgctctcgcg
     1741 cttcccgaat ctgtcgaggc gcaggtgcga ccgcgctccg gcctcgcact gcatcacggc
     1801 gtcacggtgc tcaatgcgcc gggaaccatc gacagcgact atcgcggcga ggtgatggcg
     1861 atcctcatca atctgggcga gaatccgttt cgcatcgcgc gcggaacgaa gatcgcgcag
     1921 ctgattattt cgctccacgc gcgcgccgag cttgtggaat gttcggaatt gccgcccagc
     1981 gcccgcggcg cgtccggatt cggctccacc ggctccggcc gcacactgct ttagtgtatc
     2041 gattcagaca ccaggcaccg cacctgactg gccgggcgcg ccgcacgaag ccggtgaacc
     2101 gccaaggcgc caagccgcca aggtgcgcgt gaagcgatga acaatcacaa tgaggcgggg
     2161 agacagcaag gagtctttgt gcgcctgcgg cgcgctaggt tctctcaccg cctcactgtc
     2221 tcattgtgaa tatcttggcg tcttggcggt ctacaaattt ggcgcttcgg gactcgtccg
     2281 ccagaatttg accactcgtt caactcataa catcagatca ggccggtcgg tgcagatggc
     2341 gtcgatgccg gccgcgtcga gcgttctcgt ttgcgccgga tcgtcgaccg tccacgcgcc
     2401 cactttgagg ccaaggcgat gggcttgtgc gatggcgtcc gccgtcgcat cggcggcata
     2461 ggggaaccag ccgtcgccgc ctgccgccgt aatcgcttcc agaatcgagc taccatgatt
     2521 gacggcatcg aagcctgcgg cccaggggga cgtgccggtg catgcccaat ggcgcagcat
     2581 ctggagcgcg ggttgtggcg gcggatcgtc ttgcggaggc ggctcgccgt cgcgaaacca
     2641 gctttgcggc cgcgtcgagt accagcattc caccgcgggt tcgacggatt tcgcgcggcg
     2701 caaaccgcgc cagtcgaatc ccacgatgat cgaacgatcg acgtagccag agttgcgcaa
     2761 tacggcgagc gtcgcttcgg cgaggcgttc gggcgaagct gacggtgtgg gatctgcgag
     2821 cgaggttttc agttcgatga atagccggaa aagcttcggg gcgcgtcgcg cgagcgcgac
     2881 cacctcggcg agcgcgggaa tgcgcgcgcc atcgcaggca atcattgccg gatgttcgcg
     2941 cgcataagcg ctttccggac gcgcccggcc gacgtcatag agcgcaaatt cgccggcggt
     3001 gagatccacg atccttggcg tggggggcac gagccatttg ccgtccgcat cccggcacaa
     3061 atccggggaa agacggaaat cgtgaaacac gacgacatcg ccgtcgcgcg tcagctggac
     3121 atcgagttcg gcgccgtccg ctccgagcgc gatcgcattc gcgaacgcac agagtgtgtt
     3181 ctccggcctc agccaggccc ccccacgatg ggcgattcga agcgccggca tcagaacgct
     3241 gaccgctggc gatccgcgtc acgaaccaaa accggagaat ccatggctgg cgtccttgga
     3301 ttggcggcgc tgcgactgta acgtccggcc cgccagaggc gagacaagag aggctggaga
     3361 cgaacatgag attagcgcgt gaagccgcga tcgcggcctt ttttttgctt gccgcgaacg
     3421 cggccgccgg cgcggccacc gcgccgcccg cacccaaaaa agtcggacca gacgttttgt
     3481 tcgaacaagg gttgttcgac gcggcagaag cgggctacca ggcggtttca aagacgagcc
     3541 cgggctatga gggcgcactg cggcaactgg gcgccatcgc gctttaccgc aaccatcccc
     3601 aggaagcgga attgaggctg cgcaacgcgc ttgcccacaa tcccgcggac gcgcgctgtg
     3661 ctggtctcct cgccgagacg ttgaatcgcg agggcaagtt tgccgagatg gcgcaattgt
     3721 tgcgtcagat cggcaggccg gagcgggcgg cggaattcga gctgttcgcc agaatccagc
     3781 cctatcgcct gcgcggacga gccgcgcctg tcacgatccc cttcgagcag accaatcccc
     3841 tgcctgccgt ccgggcaaag gtgaacgatc tcgaaggttt gttcctgatc gatacgggcg
     3901 gcgccgagat tcttctcgac cccgaattcg ccagggaagc gcatgtggca acgacggcaa
     3961 atgcgcaggg cccgtcttcc gccggaaggc ccgccgcgcc gtcgttcgga cgcatcgcgc
     4021 aatttgcgct gccgggcctc cagaccgacg atgtccccgc aatgctgcta agcacgaagg
     4081 cattctccag catggcgcgg ggcaaaaaag tggcgggcat catcggaacc gaatacctgt
     4141 cgcagttccg cgcgacgctc gattatgtgc gggatcgact gatcctcgag ccgcgggagg
     4201 cgcctatgcg ctccggggcg cgcaccgccg agattccatt ctggatggtg ggagatcatt
     4261 tcctgctggc gcaaggccgc ctcgatcggg gaccaaagca attgctgctc gtcgatacgg
     4321 gatttggacg gcttgccttc accgcaccag aatcgacact gcgagatgcg ggaatcacca
     4381 tgcccgcgcc acaggcggga ccgtcgggac ggccgccgat tgcaaaattt ccgatcgcgc
     4441 aactatcgct cggaagcctg gaggaaaaga acatcagcgg tatttacggg gcttttccgc
     4501 caggtctcga aaacgggctg ggcgtccatg tgggcgggat cgtcagccat gaatattttc
     4561 ttccctacgc ggtcacgttc gatttcgtcc gaatgaaaat cgggattcgc aagtaggtgc
     4621 cgctcatcct gtgaccgcgc agccacgcgc gcggagaatg cgcaagacgt cctggccctg
     4681 tgttgacaga gccaggcctc cagcgtagct acccagccga tccgcaggcg tcgtgtctgc
     4741 ggacgtttcg agcgagctgt tcaatggaaa tctgcaattg ccagatttgc atgccgccga
     4801 cgccgttcga ccttgtggtc ggcgtcaccg ctgcggcggc tcgtgaggag aacgtggcct
     4861 agtttctgcg caacgttcaa acgtttccct cccgagcccg gaaggcgctt cggaggtttt
     4921 tttgtcactt ccgaaattgc cgtccgggcg cgggcctgaa agacaagacc gtgccatgtc
     4981 cgatattgca gaattcgatc ttgcgcatgc cggccggccc gtaacgggcc gcgggctcga
     5041 cgcgtggatc gccgaagcgc gcgagctcgc gaaactcgca gcgcctctgg tgctgaccca
     5101 gcttgcccag atggcaatcc tgacgacgga tgtcgtcatg ctggggcggc tcggcaagac
     5161 ggcgctcgct tcggcggcac tcggcaatac ggtgttcttc ttcacctggc tcatcggctg
     5221 cgggccggtg gcagccgtag cgccgatggt cgctcatatt cttggagcgc gacccgggaa
     5281 tcgcgccggc gtgcgcaacg cggtgcgcat gggcttctgg gcgatgctgc ttttgtccgc
     5341 gccgttgatc gtgtttttgc tgttcacgca gcccatcctg ctcgcgctcg gccagagacc
     5401 cgagcttgcg gcgggcgcgg gccgcttcac cggtatgctg tgctggggcc tgccgttctc
     5461 gctcggctat caggtgttgc gcaatttctc gaccgcgctc tcgcggccga acgcgtcgct
     5521 atgggcgatg ggactcggca tcttcttcaa tgcggtgggc gattacgcct tgatcttcgg
     5581 ccatttcggc ctgccgagac tcggactggt cggtgccggc atcgcgagcg cgtgctccta
     5641 cgccttcgcg ttcttcatga tgctcgccgt cgtccggctg atgccggcct tgcacaagtt
     5701 ccgcatcttc cggcgctttc atcgcctgca ttgggacaag cttgtggagg tgttccgcct
     5761 cggcatgccg atagggctga ccatgatctt cgaggcgatg ctgttcaacg cagcaactct
     5821 gatcatgggc acgttcggca cagcgtcggt ggccgcgcac cagatcgccc tcaacgtgcc
     5881 ttcgatcacc ttcatggtgc cgctcggcat cggaatggcg gcgacggtgc gcgtcggact
     5941 tgcggcgggc gcgggcgacg gcgatgccgt tcgccgcgcc ggatacagcg cgctgctcat
     6001 gggcgccgga ttcatgagca tgacagcgat cgtgctgtgg atgttcccct ctgcgatcgc
     6061 caatctttat tttgccgacg acgccgccaa cgccgacgtg atcgtgcttg ccgtcgtgtt
     6121 cctgcgtgtc gcggcggcgt tccaggtctg cgacggactg caggtcgtgg gtgcgctcag
     6181 tctgcgcggc ctgaaggacg cacacatgcc gatgtggatc gccgggctga gctattggct
     6241 cgggggattt cccgtttgcc tgtggctcgc cttcggctgg catctgaagg gtcttggcat
     6301 ctggatcggt cttgccacag gcctgttcgt cgcggcggcg ctcatgtgct ggcgcttttg
     6361 gtttctgtcg aaggcccggt aaacggggtg cattgaccat tcacgatcgc gtgagacggg
     6421 cttgccggcc cggcgggaac gccgtctgga gccggaatcc cggacaaacg gccttccaat
     6481 atccccgggc cctgttggga attgtcccca atagggatta ctattgcttc gagctcggag
     6541 attccgcagt ttagatgcgg ggaggagctg tcaagatgcg caaggttgcg ggggtttttg
     6601 cggcgatcgc tttgggggca ttgcttcctc tgtggacggc caacgcgcaa ctgcccgcgg
     6661 cgggcgcccc cctcggatct aagagcgaaa ctcccggcgc ctttcccgac gtgatacccg
     6721 gcgacaatat cctgatcgcc tgcgacgcgg ttgagaagcg cgccgcagat tcggacgtgc
     6781 gggtcgtgct taccatttcc gcgatgccgg gcgaatcgac aaccggatac gccaaggttc
     6841 tggcgaccga cgaactgctg ctgaaagacg cagtccgggt gcgcattcct ctgataccga
     6901 acctcgaaca acacacggta gggctcgacg tgtatgtcgt cggttcggcc aacgccgaac
     6961 attgcgacgc cggccacatg aagatctcgt agagctaccc cggcgcagta ccgaactccc
     7021 gctccgcagt cacgcgcctg tgcggcgtcc gttggccgcc tcggtgcggg cgcgcgtttc
     7081 tttgatcgcc gacagcgagg ccgccgtgag actcgtcgat ccgtcctcca ccagctcgcc
     7141 catcagctct gcgaaacgcc tggtgtgctc ggcatagtcg cgcgcgaagt cgagcatcca
     7201 ctcctgctga atcgccagca ggcccgatac gccctggcaa tcgcggctgc tttcgatggc
     7261 gtggccggta tgctcgagac gccggttgac gaatttcagg gattcttcct gcaccgcatg
     7321 catcatcttt tgaagcatcg tgcggttatg ttccatcacg cgcgccatcg gaccgccgaa
     7381 cgcgtcgaat gcgtccaaca ggctctgccg ggtctggccg gtcggcatcg cgccttcgct
     7441 ttcctcgttc ttcatctcaa attcctcacc catgttcccg ctctcttcgc ccgtgtaacg
     7501 gcccctctgc ttggctctcg ccatggcttt gactcctgtt cgcggatgat cacgcccttc
     7561 gattgctgct gcaacgtcgc gggcgtgcat ccgttccgca gtggcaggat caaattcgcc
     7621 aggcctgcaa tccgttcgga cggtcacggg cgaaatcaga gaacgccgat ggcgagtatc
     7681 gccagcgcac ccgctgcgat ccacaacgcg acgcggacgt gacgcgtgcg tgcaacctgg
     7741 gcttgcgcga tctggcggac agaatcggga tggagtttca atccgccgtc ggccagcatc
     7801 gtcgaaatga tgtccgcatt gcgcaacaga tgcggaaaat cgcgcgccag ccggccaagc
     7861 gcgcttacgc cttccgcggc atcgcgcagg cgcgcctccg gtccgatgcg ctcgatcatc
     7921 cactcctcga tgacggggcg cgaagcttcc cagatgtcga attccggatc gagagcacgc
     7981 gcaacgccct ccaccacaac catcgtcttc tgcaggagca caagctgggg ctgcagttcc
     8041 atgtcgaaac gccgcgtggt gtcaaacaat tgttgcagaa gcttcgccat cgacacgcag
     8101 cttgcccgcc ggccgaacac cggctcgccg atcgcgcgca gagcctgcgc aaagagcggc
     8161 gcaggatggg tgcgggggac gaagccggcg tcgtaatgga cgttggcgac tcgctcatag
     8221 tcgcgcgcca gaaagccgcc aagcgtctcc gccatgaaac gtcgcatcgg ctcgtcgagc
     8281 cgccccatga tgccgaaatc gacgcagacc aaccggctct cggcatccac gaagagattc
     8341 ccctggtgca tgtcggcgtg aaagaa
//
LOCUS       DASZMC010000243         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5038295,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000243 DASZMC010000000
VERSION     DASZMC010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5038295"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VG274_04925"
     CDS             complement(<1..348)
                     /locus_tag="VG274_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06029.1"
                     /translation="MAQPRRRPTKPSARRSVFLNCPFDDEFKELYRAIVFAIVASGYT
                     PRCALERLDGGEYRLDKIAGMIGACDWGIHDISRVEVDPKNPAPRFNMPMELGIHLGA
                     RLLGQGRHRRKRAL"
     gene            complement(583..1860)
                     /gene="ccrA"
                     /locus_tag="VG274_04930"
     CDS             complement(583..1860)
                     /gene="ccrA"
                     /locus_tag="VG274_04930"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HWE06030.1"
                     /translation="MSAEARRTLEVYKDLYDIGEIPPLGHVPRQMHAWTIRKDRHGAP
                     EQSMRIETVPTWPLDSHDVLILVMAAGVNYNGVWAALGTPISPIDAHGHPFHVAGSDA
                     AGIVWAVGSKVKRWKVGDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSFA
                     QFARVQDRQLMERPRHLTWEESACYTLTLATTYRMFFGHRPHVLRPGHNVLIWGSSGG
                     LGSFAVQLCAVSGANAIGVISDESKRDFVLSLGAKGLINRSEFHCWGAMPRVGTPEYD
                     AWTKEARRFGKAIWAITGKGRDVDMVFEHPGEQTFPVSCLVVKRGGMVVFCAGTSGYN
                     ITFDARYVWMRQKRIQGSHFANLNQASQANRLMLERRLDPCMSEVFPWEDIPRAHTKM
                     WKNEHLPGNMAVLVNARRPGLRTFEDVIEASRG"
     gene            complement(2091..3308)
                     /locus_tag="VG274_04935"
     CDS             complement(2091..3308)
                     /locus_tag="VG274_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HWE06031.1"
                     /translation="MIDTQAIRQRWEFVGSKFDERGRRVFAAGEARAAGWGGVAAVSA
                     ITGLARSTIGRGLKDLEAAPMPKGRERRKGGGRRRLSSQDATLIEDLRRVIEPATLGD
                     PMRPLLWVSKSHDKVADALQKQGHEISASSVKRLLPTLGYSRQSNRKADEGSKHPDRN
                     AQFEHINAKAIAAQAAGQPIISVDTKKKELIGNYKNGGTDYCPKGDPRRVKVHDFEDK
                     ELGKVVPYGVYDVGANAGWVNVGITSDTAQFAVASIRRWLDAMGRRRYPKARELTITA
                     DGGGSNGSRVRLWKVELQKFADETGLVLNIHHYPPGTSKWNKIEHRLFCHITQTWRGR
                     PLVDRMTVVELIAATTTKAGLKVESALDTATYQKGIKVSNAQMKTLDIQGDTFHPEWN
                     YTIRPRSAANRSG"
     gene            complement(3360..>3631)
                     /locus_tag="VG274_04940"
     CDS             complement(3360..>3631)
                     /locus_tag="VG274_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06032.1"
                     /translation="PYATPPRKPPRRQGAKTRDSPQRHGRHGKKKESRALAREFSSVL
                     SVVSAVAIFFPPLAALASWRFNLSFLLRTSVTTNESDRARRPVVI"
BASE COUNT          623 a         1185 c         1104 g          719 t
ORIGIN      
        1 gagcgcacgt ttgcgccggt gtcgtccctg ccccagtagc cgcgcgccca gatgaatccc
       61 gagttccatc ggcatgttga agcgcggcgc cgggttcttc ggatcgacct cgacgcggga
      121 aatatcatgg atgccccagt cgcaggcgcc gatcatcccg gcaatcttat cgagacggta
      181 ctcgccacca tcgagccgtt ccagggcgca gcgtggggtg tatccggagg cgacgatcgc
      241 aaaaacgatc gcgcgataaa gttctttgaa ctcgtcgtcg aaggggcaat tgagaaagac
      301 ggagcgcctg gcgctcggct tagtcggccg acgcctgggc tgggccacgc tggatcacct
      361 tgcgcattgc ttcgccaatt tctttcttgc gcttggaatt cgtccaggtg cgcgtaatcc
      421 gaacgcctcg atagacgaag ctgtctggcc cggcactgcc gaaggccttt gcacgcttct
      481 tggcggctgc tctcagtgcc atatcgacaa tgtacgggcg aggtacgctt ttcgcaatga
      541 atttatcagg cgcttcgggg acgagcgccc cgaaaggccg tactaccccc ggcttgcttc
      601 gatcacatcc tcgaacgtcc gcagccccgg gcggcgcgcg ttgacaagca ccgccatatt
      661 ccccggcaga tgctcgttct tccacatctt ggtgtgggcg cgggggatgt cttcccaggg
      721 gaaaacttcc gacatgcagg gatcgaggcg gcgttcgagc atcaggcggt tcgcctggct
      781 cgcctggttg aggttggcga aatgggagcc ctggatgcgc ttctgccgca tccacacata
      841 gcgcgcgtcg aaggtgatgt tgtagccgct ggtgccggcg cagaacacga ccatgccgcc
      901 gcgcttcacc acgaggcagg acacgggaaa ggtctgctcg ccgggatgct cgaacaccat
      961 gtcgacgtcg cggcccttgc cggtgatcgc ccagatcgcc ttgccgaagc ggcgcgcttc
     1021 cttcgtccat gcatcgtatt ccggcgtgcc cactctcggc atcgcacccc agcaatggaa
     1081 ttcgctgcgg ttgatcaggc ccttcgcgcc gagcgaaagc acgaagtcac gcttggattc
     1141 atcggagatg acgccgatcg cattggcgcc cgagaccgcg cagagctgaa ccgcgaacga
     1201 gccgagcccg ccgctcgagc cccagatgag cacattgtgt cccggccgca gcacgtgcgg
     1261 ccggtggccg aagaacatcc ggtaggtggt ggcgagcgtc agcgtgtagc aggccgattc
     1321 ttcccaggtg agatgccgcg gccgttccat gagctggcga tcctgcacgc gggcgaactg
     1381 cgcaaaggaa ccgtcgggcg tttcgtagcc ccagatgcgc tgcgagggcg agaacatcgg
     1441 atcgccgcca ttgcactcct cgtcgtcgcc atcgtcctga ttgcaatgaa cgacaacctc
     1501 gtcaccgacc ttccagcgct tcactttcga acccaccgcc caaacgatgc ccgcggcgtc
     1561 cgaacccgcg acgtgaaacg ggtggccgtg cgcatcgatg ggcgagatcg gcgtgccaag
     1621 agccgcccag acgccattgt agttcacgcc cgcggccatc acgagaatca gcacgtcgtg
     1681 cgaatcgagc ggccaggtgg gcaccgtctc gatcctcatc gactgttcgg gcgcgccgtg
     1741 ccggtccttg cggatggtcc acgcatgcat ctgtcgcggg acgtgcccaa gcgggggaat
     1801 ctcgccgata tcgtaaaggt ccttgtagac ctcgagcgtg cgcctggcct ctgccgacat
     1861 ggttccctct ctgccccgct caccaaacgc gcgaaatgta tatttcgccc gcacttgccc
     1921 cctattgcat tgcagcatcc aggaggaaaa gcaaaaagat tctagcgcag aggacgcaga
     1981 ggatgcgcgg tggacgcaga agagctttgc gcgcctctgg cgcgcggttc ttcttccacc
     2041 gcgtcctctg cgcatcctca gcgtcctctg cgctaaggac acccgtcaat ttaaccgctc
     2101 cgattagcgg ctgaacgcgg gcggatagtg tagttccact cggggtggaa tgtgtcgccc
     2161 tggatgtcga gcgtcttcat ctgggcattg ctgaccttga tgcccttttg gtaggtcgcg
     2221 gtatcgagcg cgctctcgac tttcaggccg gctttggtcg tcgtcgcggc aatcagctcg
     2281 acgacggtca tgcgatcgac gagcggtcgc ccgcgccagg tttgcgtgat gtggcagaac
     2341 aggcggtgct cgatcttgtt ccacttcgac gtgcccggcg gatagtgatg gatgttgagc
     2401 acgagaccgg tctcgtcggc aaacttttgc agctcgacct tccacagccg gacacgcgat
     2461 ccattcgagc cgccgccatc ggccgtgatc gtcaactcgc gggctttcgg gtagcgccgg
     2521 cgccccatcg cgtcgagcca gcggcggatg gaagcgacgg caaactgagc cgtgtcgctt
     2581 gtgatcccga cgttcaccca cccggcattg gcgccaacgt cgtacacgcc ataaggcacg
     2641 accttgccga gctccttgtc ctcgaagtcg tgcaccttca cgcgccgcgg atcgcctttc
     2701 gggcaatagt cggtgccgcc gttcttgtag ttgccgatca gctctttctt cttggtgtcg
     2761 accgagatga tgggctgccc cgcggcctgt gcggcgatcg ccttggcgtt gatgtgctcg
     2821 aattgcgcat tgcggtcggg atgcttcgag ccctcgtcgg ccttgcggtt cgactgccgg
     2881 ctgtagccga gcgtcggcaa cagtcgtttc acgctactgg cgctgatctc atggccctgc
     2941 ttctgcaacg cgtccgccac cttgtcgtgg cttttcgata cccacaacag cgggcgcatg
     3001 ggatcgccga gcgtggccgg ctcgatgacc ctgcgcagat cctcgatcag cgtcgcgtcc
     3061 tggctcgaca gacgccgccg cccgccaccc ttgcgacgct cccggccttt cggcatcggc
     3121 gcggcctcga ggtctttcag tccccgaccg atggtcgagc gcgccagccc cgtaatcgcg
     3181 gagactgccg ccactccgcc ccatcctgcc gcccgcgcct cgccggctgc gaacacccgt
     3241 cgtccgcgct cgtcgaactt cgagccgacg aactcccatc tttgccgaat cgcttgcgtg
     3301 tcgatcatgc cgacccgcat accgccccag tgccatcccg agaatcccgc cgacccgagt
     3361 caaatcacca caggccgccg agcacgatcc gattcatttg ttgtgacgga tgtccttagt
     3421 agaaaagaaa gattgaaccg ccaggacgcc agggcggcaa ggggcggaaa gaaaattgcc
     3481 accgcagaga caacggagag cacagaggaa aattcgcgcg ccaaggcgcg cgattccttt
     3541 ttctttccgt gtcttccgtg cctttgtggt gaatctcttg tcttggcgcc ttggcgtctt
     3601 ggcggttttc ttggcggcgt cgcgtacggc g
//
LOCUS       DASZMC010000244         4040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2854561,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000244 DASZMC010000000
VERSION     DASZMC010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854561"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..1085)
                     /locus_tag="VG274_04945"
     CDS             complement(381..1085)
                     /locus_tag="VG274_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06033.1"
                     /translation="MRMSNGIGRREEIVSVCRKLSNRQYVSRRRIPLKGCRVLKRLFI
                     GWSYCAGQLSTGHGSMNMSSIAAVAALGIAVSGCATIVTGTSQSVAITTPPTTGAECV
                     LSGKEGSRTVVSPGATRVEKSKEDIAVHCSKPGWQDAEATIPSHFQGWTFGNIAIGGL
                     IGAGVDAATGAMHKYPRAFEVPMMPNHDGNSSPGYNSPPPSGAQTSANDVGGAGLQRF
                     DQWQGQRAQTSAVSPK"
     gene            1284..2552
                     /locus_tag="VG274_04950"
     CDS             1284..2552
                     /locus_tag="VG274_04950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWE06034.1"
                     /translation="MAKADRRMVDRMRMTTDVAVIGAGPSGSVAAAYLAARGYSVEVV
                     ERQHFPRFSIGESLLPQAMVFLEEAGLLDCVVAAKFQYKNGAVFRRGDEEQSLDFRDK
                     SAPGWGTTFQVRRDLFDHVLANAAAEKGARIAFGEEVIRFAPGDDRVWMLVRNESGSE
                     REIAARFALDASGFGRVLARLLSLDRPANFPVRKAVFSHVRDHVSDVTFDRDKILVSV
                     HPQNSDVWYWLIPLADGLSSIGVVGTEADLSAAGADNQAQLVNLLKSAERMSQVLENA
                     EQIRPAGTITGYACNVDRLSGPGFALLGNAAEFLDPVFSSGVTIALKSAILAGRILDR
                     KFRGEIVDWENEFERPLKVGIETFRAYVEGWYDASLQDVIFLQPKRRTDVTRKIISVL
                     AGYAWDLENPFVREPRRHLAAVHQLVAARA"
     gene            2574..3722
                     /locus_tag="VG274_04955"
     CDS             2574..3722
                     /locus_tag="VG274_04955"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HWE06035.1"
                     /translation="MKPIYIQEFAIACPLGEDAQTVRTNLLSSAPTAIHDRADLTDGR
                     SVPAGVLRFDLSGRESRCNLLADRCLDALNGAVARQMEQTGPERFAVIIGTSTSGIRE
                     GGAAFEAFQQSGRWPDGFRFSGQELGDTAHYVARRIGARGPVYGISTACTSGAKALAS
                     AARLIEAGLCDTAVAGGIDSLCDLTLNGFAALEALSEAVCNPFSKNRRGLNLGEGGAL
                     FLLSAAPSPVRFAGWGESADAHHISAPEPEGKGVAAAMERALMRASLAPQAIGYLNLH
                     GTATRLNDLMEARAVMQVFGGALPCSSTKPMTGHMLGAAGACEAAFSIMALQAGRLPA
                     HIWDGESDPGLAPIRLVSRNGEVSDARRAMSCSYAFGGNNIALILEAE"
     gene            3719..>4040
                     /locus_tag="VG274_04960"
     CDS             3719..>4040
                     /locus_tag="VG274_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06036.1"
                     /translation="MTDTTTSIDELMVHRLPMRLLDRIVACSECEVVAEACVRADNLF
                     FEPGRGLPPYVGLEMMAQAIAAIDGKKRKSSGKPAKIGFLLGCRCFASRVPCFAEGTR
                     LTVLA"
BASE COUNT          738 a         1247 c         1252 g          803 t
ORIGIN      
        1 tggaagtcag cgggcctgct aaaacccctg ctatcaagcc catcttcgca acgctcgaac
       61 gatcccttgt catccgtcgg ctcggcgctc tcgttctcgc cgacggcgaa gctctccgcc
      121 aagccattgc gcagacgttc gggtgattgc aacgcgcccg gttcagccgc cccaaatgct
      181 ggcgcattgc gattccgacc cccaaatccg ccctttgatt cagattcgcg ctttccgcgg
      241 aatccccttc atatcccacg agtcaactcg ccgcatgggg gtgagcaagt accaaaggcc
      301 cggcaaccca cggactttgt cttgtagatt ggttgagcgg atgggctcgg gcaaaaagtc
      361 cgcgagggca agcaagctac ctacttggga ctgaccgcac tcgtctgtgc gcgctgtcct
      421 tgccattgat cgaatcgctg caggccggcg ccgccgacat cgttcgcaga cgtctgcgcg
      481 cctgaaggag gaggtgaatt atatccgggc gaggaattgc cgtcatggtt tggcatcatc
      541 ggcacttcga atgcgcgtgg atacttgtgc attgcgccgg tcgctgcgtc cacgccagcg
      601 ccgatcaagc cgccgatcgc aatgttgccg aaggtccaac cctgaaaatg agacggaatt
      661 gttgcttcag cgtcttgcca acctggcttg ctgcagtgga ccgcaatgtc ttccttgctt
      721 ttctctactc gggttgctcc cggcgaaacg acggtccggc ttccctcctt gcccgacaaa
      781 acgcattctg caccagtggt cggaggtgtc gtgattgcga ccgattgcga tgttcccgta
      841 acgatggttg cgcagccaga aactgcgata ccaagcgcgg caacggccgc gatcgaagac
      901 atgttcattg agccgtgccc cgttgaaagc tggcccgcac aatatgacca accaataaac
      961 aggcgcttaa gaacacggca gcctttgagg ggaatacggc gacgggatac atattgccga
     1021 ttggaaagct tcctgcacac agagacaatt tcctcccgtc ggccaatccc gttgctcatt
     1081 ctcatgtcaa attatgaaaa cgagtcctac cgccggattc taggtcccct ctgcacatcg
     1141 aaagcgtggg tctgctgcga aatcccgttg cctgcttcca attggcatgg ttggacaggc
     1201 tcaccgtgag ggccttatcc ttagcctgct cgcctgcgct aaagcttcgg tgggcctgta
     1261 cttttctcat atccgccgaa gccttggcga aggcggatcg aaggatggtc gatcgcatgc
     1321 gcatgaccac cgacgtcgcc gtgatcggcg cgggcccatc gggcagcgtt gcggccgcct
     1381 atctggcggc gcgcggttat tcggtggagg tcgtggagcg gcagcatttt ccgcgcttct
     1441 ccatcggcga aagcctgctg ccgcaggcga tggtctttct cgaggaggcc ggcctgctcg
     1501 actgcgtcgt ggcggccaaa tttcaataca agaatggcgc cgtattccgg cgcggcgatg
     1561 aagaacaatc cctcgatttc cgggacaaat ccgcgccggg atggggcacg acgttccagg
     1621 tgcgccgcga tctcttcgat catgtcttgg cgaacgcggc ggcggaaaaa ggcgcgcgga
     1681 tagccttcgg cgaggaagtg atccgtttcg cgcccggcga tgaccgcgtg tggatgctgg
     1741 tccgcaacga gagcgggagc gagcgcgaaa tcgcagcgcg cttcgcgctc gacgccagcg
     1801 gcttcggccg cgtgctcgcg cggctgctgt cgctcgaccg tcctgccaat ttcccggtcc
     1861 gcaaggccgt gttcagtcat gtgcgcgatc atgtttccga tgtcactttc gaccgcgaca
     1921 agattctggt cagcgtgcat ccgcaaaaca gcgatgtctg gtactggctg attccgctgg
     1981 cggacggcct gtcgtcgatc ggcgtggtcg ggacggaggc ggacctcagc gccgccggcg
     2041 ccgacaatca ggcccagctg gtcaatctcc tcaaatcggc cgaacggatg agccaagtgc
     2101 tcgaaaacgc cgaacagatc aggccggccg gaacgatcac gggctacgcc tgcaatgtgg
     2161 atcgtctttc gggtccgggt tttgccctgc tcggcaacgc ggcggaattt ctcgatccgg
     2221 tcttctcgtc gggcgtcacc atcgcgctca aatcggcgat cctcgccggc cgcattctcg
     2281 accggaaatt caggggcgaa atcgtcgatt gggagaacga gttcgagcgg ccgctgaagg
     2341 tcggcatcga aacctttcga gcctatgtcg aaggctggta cgacgcctcg ctgcaggacg
     2401 taatcttcct gcagccgaag cgccggaccg atgtgacgcg caagatcatc tccgtgcttg
     2461 cgggttacgc gtgggatctg gaaaacccgt tcgtgcgcga gcccagacgc catcttgccg
     2521 ctgtgcacca actggttgcg gcgcgggcgt gacggcgcag ataaaagcgg atcatgaagc
     2581 cgatctacat ccaggagttc gccatagctt gcccgctcgg cgaagacgcg cagaccgtgc
     2641 ggaccaattt gctttcatct gctcccactg cgattcacga ccgcgcggat ctcaccgatg
     2701 gccgttccgt tccagcggga gttctgcgct tcgacctgtc cggccgcgaa agccgttgca
     2761 accttctcgc cgatcgctgc ctggatgcgc tgaatggagc cgtcgcacgc cagatggagc
     2821 agacgggccc cgagcgattt gccgtcatca tcggcacgtc caccagcgga atccgcgaag
     2881 gcggagcagc tttcgaagct ttccagcaga gcgggcgttg gccggatgga ttccggttct
     2941 ctgggcagga actcggagat acggcccatt atgtggcccg ccgcatcggc gcgcgcggac
     3001 cggtgtatgg aatctcgacc gcctgcacct cgggtgcgaa agctttggcg agtgcagccc
     3061 gcctcattga ggccgggctc tgcgacacgg ctgtcgcagg cggcatcgat tcgttgtgcg
     3121 acctgacgct caacgggttc gcggcactgg aagccctgtc ggaagcggtc tgcaatccgt
     3181 tcagcaaaaa ccgccgcgga ctcaatctgg gcgaaggcgg cgccctgttc ctgctctccg
     3241 ccgcgccgtc gccggttcgc tttgcgggct ggggcgaaag tgccgatgcc catcacatca
     3301 gcgctccaga gcccgaaggc aagggcgtcg cggccgcgat ggagcgcgca ctcatgcgcg
     3361 cgtcgcttgc cccgcaggca atcggttatc tcaatcttca tggcacggcc accagactga
     3421 acgacctgat ggaagcgcgc gccgtgatgc aggtgtttgg cggggcactg ccgtgcagtt
     3481 cgacgaaacc gatgacgggt catatgctgg gcgccgccgg cgcgtgcgag gccgccttct
     3541 ccatcatggc gctgcaggca ggccgccttc ccgcccatat ctgggatgga gaatccgacc
     3601 cggggcttgc gccgatccgg cttgtgtcac gaaatggcga ggtgagcgat gcgcgccgcg
     3661 cgatgagctg ttcctacgca tttggcggca ataacatcgc gctgattctg gaagcggaat
     3721 gacggatacc acaacctcca tcgacgaact gatggtgcat cggctgccga tgcgcctgct
     3781 ggatcggatt gtcgcgtgca gcgaatgcga ggttgtagcc gaagcgtgtg tgcgcgcgga
     3841 caacctgttt ttcgaacctg gccgcggcct tccgccctat gtgggtctcg agatgatggc
     3901 ccaggcgatc gcggccatcg atggcaagaa gcgaaaaagc agcggcaaac ctgccaagat
     3961 cggtttcctg ctcggttgcc ggtgctttgc atcgcgcgtc ccctgctttg ccgaagggac
     4021 acggttgacg gtattggcga
//
LOCUS       DASZMC010000245         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1219966,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000245 DASZMC010000000
VERSION     DASZMC010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219966"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VG274_04965"
     CDS             <1..330
                     /locus_tag="VG274_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWE06037.1"
                     /translation="RSWVRKGLEAWFTVLMETFWPKRRILIVYLNIVDWGHGNFGAEA
                     AARAYFHKSADQLTVSQAARLAAILPDPGDWRAARPGPYVKHRSATLLSRMAAVRRDN
                     LESCLRR"
     gene            599..1243
                     /locus_tag="VG274_04970"
     CDS             599..1243
                     /locus_tag="VG274_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE06038.1"
                     /translation="MRRLVQLLLAPPSRFARLLVAEKRLTCDCVAPEDLAEHLPVFVD
                     LDGTRCEGLWAIVDHLEGVYPDHPLAPEDDEARRESLRLLDWAMGPFLESVTKRIVYE
                     RASQRYTGAASRRAPDMDNVRSGREALRAALTTLGASAERHGYLTTRDCTLGDLAVAA
                     HLSAIDYFGEVPWTEFPAAAEWYMRMKSRPSFRNLLADRVPGQPPVSHYADLDA"
     gene            1224..2369
                     /gene="queG"
                     /locus_tag="VG274_04975"
     CDS             1224..2369
                     /gene="queG"
                     /locus_tag="VG274_04975"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWE06039.1"
                     /translation="MRTSTPDHGTRETIRARAGELGFDVVRFARAAAPEGAGKHLAQF
                     LVEGRHGTMDWLAKNADRRARPSALWPEARSIVVLGTNYGPVDDPLAQLGDSARGAIS
                     VYAQGEDYHDVLKSKLKALGEFIAEQFAADVKIFVDTAPVMEKPLAQAAGLGWQGKHT
                     NLVSREFGSWLFIGSIFTTIEIESDESETDHCGSCRRCLDICPTQAFTAPYQIDARRC
                     ISYLTIEHKGHIAREFRAAMGNRIYGCDDCLAVCPWNRFASVAREAKFHARAELASPS
                     LADLSRLDDAAFRSLFRNSPVKRTGRDRFIRNVLIAIGNSSDAALAPEAQRLLSDASP
                     LVRAMAVWALGRLLDPPAFTALRETHSSREPDSDVRHEWEDALAADK"
     gene            complement(2603..3406)
                     /locus_tag="VG274_04980"
     CDS             complement(2603..3406)
                     /locus_tag="VG274_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE06040.1"
                     /translation="MRVLSRFIKVACLAAAALIAGACDAFAAKASPLDDPALGGRNRY
                     DRCLVLVKRDAARAETEAAGWSAAGGGAAALHCEALALVELKRFAEAAQKLEDAANQS
                     AAAPSLRAELLDQAGNAWLLAGAAGKAESALSAALALEPKSEDVLADRARERGAAKNW
                     TGAVGDLTSVLALDPDRADIYVLRASALHAQGRKAEAGADIGRALGIYPDYPEALVER
                     GVMRLETGDSAGARADWLKTAQEAPDSDAASAALAHLKMLGAATSPAKR"
BASE COUNT          575 a         1229 c         1120 g          646 t
ORIGIN      
        1 cgaagctggg tgcgaaaggg actcgaggcc tggttcaccg ttctgatgga gacgttctgg
       61 ccgaaacgcc gcatcctcat cgtctatttg aacatcgtcg attggggcca tggcaatttc
      121 ggcgccgagg ccgcggcgcg cgcctatttc cacaaatccg cggaccagct gaccgtctcc
      181 caggccgccc gcctggcggc cattctcccc gatcccggcg actggcgcgc cgcccgcccc
      241 ggcccctacg tcaaacaccg ctccgcgacg ctgctctccc gcatggccgc cgtccgccgc
      301 gacaacctcg aatcctgcct gcgccggtag tgctccaaaa gccagcccgc gcgtggcctt
      361 gaaccgccaa gccgccaagg gacacatacc aaactgacga ttcacaagga gacagtgagg
      421 cggggaggag gaatggcgcg cgaaggcgcg caacaattct caccgtctca ctgtctcctt
      481 gtgaatcttc ggcacggcac gggcgttttg gcggcttggc ggttcaagat ccggtcaagg
      541 agccatgcgc cggaatttga ccctaggagc gacccgcccg cttcattaga ttgcgcggat
      601 gcgccgcctg gttcagttgc tgcttgcgcc gccgtcgcgg ttcgcccggc tgctcgttgc
      661 cgagaagcgg ctcacctgcg actgcgtggc gccggaagac cttgccgagc atctgccggt
      721 gttcgtagat ctggacggca cgcggtgcga aggattgtgg gcgatcgtcg atcacctcga
      781 aggtgtctat cccgatcacc cgctggcgcc ggaggatgac gaggcacgac gggaatcgct
      841 gaggttgctc gactgggcga tggggccgtt tctggagagc gtgaccaagc gcatcgtcta
      901 tgaacgggcg tcgcaacgtt ataccggcgc cgcatcccgc agggcgcccg acatggacaa
      961 tgtccgcagc ggacgggaag ccttgcgggc ggcgctcacc acgcttggcg ccagcgccga
     1021 gcgccatggc tatctcacca cgcgcgattg cacgctcggc gatctcgcgg tcgccgcgca
     1081 tctgtccgcg atcgattatt tcggcgaagt tccgtggacg gagtttcccg ccgccgccga
     1141 atggtacatg cggatgaaat cgcggccctc cttccgcaac ctgctcgccg accgcgtgcc
     1201 cggccagccg ccggtttcgc attatgcgga cctcgacgcc tgaccacggc acgcgcgaaa
     1261 ccatccgcgc gcgcgccgga gaattggggt tcgacgtggt gcgcttcgcc cgtgcggcgg
     1321 cgcccgaagg cgccggcaaa catctcgccc agttcctcgt ggaaggccgg cacggcacca
     1381 tggactggct ggcgaagaac gcggaccgcc gcgcacgccc atctgcgctg tggcccgagg
     1441 cgcgctccat cgtggtgctg ggaacgaatt acggacccgt tgacgatccg ctcgcgcagc
     1501 tcggcgattc cgcgcgtggc gcgatctccg tctacgcgca gggcgaggac taccacgatg
     1561 tgctcaaatc gaagctgaag gccctcggcg agttcatcgc cgaacaattc gcggccgacg
     1621 tgaagatttt cgtcgacacc gcgccggtga tggaaaagcc gctggcacaa gcggcgggcc
     1681 tcggctggca gggcaagcac accaacctcg tctcgcgcga gttcggctcc tggctgttca
     1741 tcggttcgat cttcaccacg atcgagatcg agtccgacga atccgaaacc gatcattgcg
     1801 gttcgtgccg gcgctgcctc gacatttgcc cgacgcaggc cttcaccgcg ccctaccaga
     1861 tcgatgcaag gcgctgcatt tcctatctca ccatcgaaca caagggacat attgcgcgcg
     1921 aattccgcgc cgcgatgggc aaccgcatct atggctgcga cgattgcctg gcggtctgtc
     1981 cgtggaaccg ctttgcctcc gtggcgcgcg aggcgaagtt tcacgcccgc gcggaacttg
     2041 cttcgccgag cctcgcagat ttgtcgcgcc tcgacgatgc cgccttccgc agtctgttcc
     2101 gcaattctcc ggtcaagcgc accggacgcg accgcttcat ccgcaacgtg ctgatcgcca
     2161 tcggcaattc cagcgatgcg gccctcgcgc ccgaggccca gcgtctcctc tccgacgctt
     2221 cgcccctcgt ccgcgccatg gccgtctggg ccctcggccg cctcctcgac ccgcccgcat
     2281 tcacggcgct gcgcgaaact cactcaagcc gcgagcccga ttccgacgtg cgtcacgaat
     2341 gggaagatgc gttggccgca gataagtaag tttgcacgcg gagccgcgga ggcgcggagc
     2401 aagatttaac cgccaaggcg ccaagacgca cgtgcagcat aaaatctcca caatgaggtg
     2461 gcggggcagt gggaattttt gcgcgctctg gcacgcgatc ctttttctct ctgcctcact
     2521 gtttccctgt gaatttttct tggcgtcttg gcgtcttggc agttcgtctt ttctgttctc
     2581 cgggctccgc gtggacctct ttctatcttt ttgcgggcga tgttgctgca ccgagcatct
     2641 tgagatgtgc gagcgcggcg ctggcggcgt cgctgtcggg cgcttcctgt gcagttttca
     2701 gccaatcggc gcgcgcgcct gcgctgtcgc cggtctcgag tctcattacg ccacgctcga
     2761 ccagcgcttc gggatagtcc ggatagatgc cgagcgcgcg cccgatgtcg gcgccggctt
     2821 ccgccttgcg gccctgcgca tgcagcgcgc tcgcgcgcag cacatagata tcggcgcggt
     2881 cgggatcgag cgcgagaacg gaggtgagat cgccgaccgc gcccgtccag ttcttcgccg
     2941 cgccgcgctc ccgcgcgcgg tcggccagca cgtcctcgct ctttggctcg agcgcgagcg
     3001 ccgcgctcag cgcgctctct gccttgcccg cagcgcccgc gagcagccag gcgttccccg
     3061 cctggtcgag caattccgcg cgcagcgacg gcgcagccgc cgactggttc gccgcgtcct
     3121 ctagtttctg tgccgcttcg gcaaagcgtt tcagctcgac aagcgccagc gcctcgcaat
     3181 gcagcgcggc cgcgccgccg cctgcggcgg accatccggc cgcttccgtt tcggcgcgcg
     3241 ccgcgtcgcg cttcaccagc acgagacagc ggtcatagcg gttgcggccg cccagcgcgg
     3301 gatcgtcgag cggcgaggct ttcgcggcga acgcatcgca cgcgcccgcg atgagcgccg
     3361 cggccgccag acaagcgact ttgataaacc gggacaaaac ccgcatggtt cgcacctgct
     3421 tgatggaatg cgacgagcga gaatagtacg cggaattccg gaagtgtatg tcacccgccg
     3481 cggccctcat ggttcgacag gctcaccatg aggacctcat cctgagcctg tcgaaggatg
     3541 aggcccgaat catgtgtcag cgatagtggg
//
LOCUS       DASZMC010000246         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2302540,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000246 DASZMC010000000
VERSION     DASZMC010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302540"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VG274_04985"
     CDS             <1..222
                     /locus_tag="VG274_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06041.1"
                     /translation="CITPSVKLNFKKIYPQVTVERSYSAGFAFWAYWLDTTNNDFSCA
                     ANQLEVITDTLDGGSVEESNLIAFSLTIQ"
     gene            355..813
                     /locus_tag="VG274_04990"
     CDS             355..813
                     /locus_tag="VG274_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HWE06042.1"
                     /translation="MTVLNWTDDRVEQLKNLWTEGLSASQIARALGGVTRNAVIGKVH
                     RLGLAGRASPSRSERPRLPMAPKISVRSHIPPAPIVEEDPLVLGDGNHATVLTINDRM
                     CRWPIGDPSENEFHFCGHNPKSGSPYCEAHARKAYQPQQARRPDKGRMAG"
     gene            810..1289
                     /locus_tag="VG274_04995"
     CDS             810..1289
                     /locus_tag="VG274_04995"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HWE06043.1"
                     /translation="MSSVYDYTAQTLTGKDVSLADYRGQVLLIVNTASKCGFTPQYEG
                     LEKLYEDLHPKGLTILGFPCNQFGSQEPGGADEIGQFCQVNYGVSFPMFAKIDVNGAH
                     AHPLYKFLTYEKRGVLGTSNIKWNFTKFLIDRAGNVVARYGSLTKPEQIKSAIEKLL"
     gene            1370..>1567
                     /locus_tag="VG274_05000"
     CDS             1370..>1567
                     /locus_tag="VG274_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWE06044.1"
                     /translation="MSDWLHVSSLDWMLVVRLPIAALLGSLIGADRERLAWAAGLRTH
                     MLVCVGACLFMIVSAFGFADVL"
BASE COUNT          314 a          478 c          470 g          305 t
ORIGIN      
        1 tgcatcacgc catcggtaaa attgaacttc aagaagatct atccgcaggt gaccgttgag
       61 cgcagctatt ccgccggctt tgcgttctgg gcgtattggt tggacacgac caacaacgac
      121 ttcagttgcg cggcaaatca gctggaggtc atcacggaca cgctggatgg agggtccgtc
      181 gaggaaagca acctgatcgc cttcagtctc accattcaat gatttggggc gccggggcac
      241 gattcgcacg aatcggtatg ttgtggacaa catgtcgtgc ccccacctca ccccttgtga
      301 ctcccgattc gcttgatacg atatcttgtg gctggagcct caggggagcc caagatgacg
      361 gtactgaact ggactgacga ccgcgtagag cagcttaaga acctttggac cgaaggcctg
      421 tcggcgagcc agatcgcgcg cgcccttggc ggcgtcacgc gcaatgccgt gatcggcaag
      481 gtgcatcgcc tcggtctcgc gggccgcgca agcccgtcgc gcagcgaacg tccgcgcctg
      541 ccgatggcgc cgaagatctc cgtgcgctcg cacattccgc ccgcgccgat cgtggaagag
      601 gatccgctgg tactcggcga cggcaatcac gcgaccgtgc tgacgatcaa cgaccgcatg
      661 tgccgctggc cgatcggcga tcccagcgag aacgaattcc atttctgcgg ccacaatccg
      721 aagagtggtt cgccctactg cgaagcgcac gcgcgcaagg cctaccagcc acaacaggcg
      781 cgccgtccgg acaagggacg gatggctggg tgagttcggt ctacgactac accgcacaga
      841 cgctgaccgg aaaagacgtg agcttggccg actatcgcgg ccaggttctg ctcatcgtca
      901 acaccgcaag caagtgcggg ttcacgccgc aatatgaagg actggaaaag ctctatgagg
      961 atttgcatcc gaaggggctg accattctcg gctttccctg caaccagttc ggtagccagg
     1021 agcccggcgg cgcggatgag atcggccagt tctgccaggt gaattacggc gtcagctttc
     1081 cgatgttcgc gaagatcgac gtgaacggcg cgcatgcaca tccgctctac aaattcctca
     1141 cctatgaaaa gcgcggcgtg ctcggcacat cgaacatcaa atggaatttc accaaattcc
     1201 tgatcgaccg cgcggggaac gttgtggcgc gctacggctc gctgacgaag cccgagcaga
     1261 tcaaatccgc aatagagaag ctgctgtaaa ctcgctacgg cttcccgcag actaggcaac
     1321 gcaaccggcc accgcgcgtt tctcggatgg cgctaaggag ctgtctggca tgtccgattg
     1381 gctgcatgtt tcgtcgctcg actggatgct tgtcgtccgt cttcccatag ccgcgcttct
     1441 cggcagcctg atcggcgcgg accgcgagcg cctcgcctgg gcggcgggat tgcgcacgca
     1501 catgctcgta tgcgtcggcg cgtgcctctt tatgatcgtg tcggcgttcg ggtttgccga
     1561 tgttctc
//
LOCUS       DASZMC010000247         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2302772,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000247 DASZMC010000000
VERSION     DASZMC010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302772"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VG274_05005"
     CDS             complement(<1..261)
                     /locus_tag="VG274_05005"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWE06045.1"
                     /translation="MLATTADFPAGLIAKLQELENAVGAAILGKRDVVRMSLAALLAR
                     GHLLIEDVPGVGKTTLAQALARSVSCRFHRLQFTSDMLPSDVL"
     gene            475..1263
                     /locus_tag="VG274_05010"
     CDS             475..1263
                     /locus_tag="VG274_05010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE06046.1"
                     /translation="MTPSLNLRLDGKAAVVTGGASGIGLAIATAFASAGAAVHILDLS
                     AERAQAAVDSIAASGGRASAHPCDLSEEASASETLSAIVAKERLHILVNNVGMANIGT
                     VESTSAADFDRVMRVNVNSYFYCAKAVVGHMRESGGGVILNMASIAGSCGLPDRFAYS
                     TSKGAVVAMTFSVARDYMKHNVRCNCISPARVHTPFVDGYLRTNYPGREEEMYKVLSS
                     AQPIGRMGKPEEVAQLALYLCSDAASFITGCDYPLDGGWTNLRG"
     gene            1282..2130
                     /locus_tag="VG274_05015"
     CDS             1282..2130
                     /locus_tag="VG274_05015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE06047.1"
                     /translation="MTIDSHQHFWRFDPVRDAWITPKMAVLRRDYMPADLAPELAAQS
                     IDATIAVQADQSENETQFLLDLAALHPFIAGVVGWVDLLSPELPRRLEHFSQFKKLCG
                     FRHIAQSEPDDFLLRDDFQRGVARLADFGFTYDVLIYARQLPAAAEFVRRFPHQPFVI
                     DHIAKPDVRAREIDRWESHMRTIAAHPLTMCKLSGMITEADWRAWTPETFRPYLDIVW
                     DAFGPDRLMFGSDWPVCLLAGTYAQVKNLMEDFLRARPAAQREAIFGANAARFYSLKT
                     QHGFTA"
     gene            2114..>2612
                     /locus_tag="VG274_05020"
     CDS             2114..>2612
                     /locus_tag="VG274_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE06048.1"
                     /translation="MDLQLRNKVIIVTGGASGIGAAIVAGASREGAVAVIVDRNVENA
                     LQVECDLRKSGAQAHALFADLTSAENCRRVIDETLERCGRIDALVNNAGFNDAVGLEN
                     GNPDEYIKSLERNLFHYYNLAHFALPSLKQARGAIVNIASKTAVTGQGGTSGYASSKG
                     AILALT"
BASE COUNT          490 a          924 c          687 g          511 t
ORIGIN      
        1 cagaacatcg ctcggcaaca tgtcgcttgt gaactgcaaa cggtgaaacc ggcatgagac
       61 cgagcgcgct agcgcctgcg ccagcgtggt cttccccacg cccggcacgt cttcaatcag
      121 caggtgtccg cgcgccagca acgccgccag cgacatccgc accacgtccc gcttgccgag
      181 aatcgccgct cccaccgcgt tctctaactc ttgtaacttg gcgatcaggc ccgccgggaa
      241 gtcggccgtc gtggcgagca tcgattcccg ctgaggcatt cattttatga tactagacgc
      301 caaattccag cccgatcccg cgtagcgcgc ctcgtatagg ctccgtccgg tttttacccg
      361 ctcattcgct cagccatttc gttacaaaaa ttgcaggact gcccattctt ttacgctaaa
      421 gtgcatttga cagcgctttc agccgccgtc tatagtgaga cagtccattc atccatgacc
      481 ccttcactga acctacggtt ggacggcaaa gccgccgtcg tcacgggcgg cgcgagcggc
      541 atcgggctcg ccatcgctac cgcattcgcc tccgccggcg ccgccgttca tattcttgat
      601 ctcagtgcgg aacgcgccca ggccgcagtc gattcgatcg cagcatccgg aggccgcgcc
      661 tccgcccacc cctgcgatct ctccgaggaa gcgagcgcca gcgagaccct cagcgccatc
      721 gtcgccaagg agcgcctgca cattctggtc aacaacgtcg gcatggccaa catcggcacc
      781 gtcgaaagca cctcggccgc cgatttcgat cgcgtcatgc gcgtcaacgt gaacagttat
      841 ttctattgcg ccaaggccgt cgtcggccac atgcgcgaat cgggcggcgg cgtcatcctg
      901 aacatggcgt ctatcgccgg ttcctgcgga ttgcccgacc gcttcgccta ttccaccagc
      961 aaaggcgcgg ttgtcgccat gacattctct gtcgcacgcg actacatgaa acataacgtg
     1021 cgctgcaact gcatctcgcc cgcgcgcgtc cacacgccct tcgtggatgg ctatctgcgc
     1081 accaactatc ccggccgcga ggaggaaatg tataaggtgc tttcgtcggc gcagcccatc
     1141 ggccgcatgg gcaagcctga agaggtggcg caactcgccc tctacctctg ttccgatgcc
     1201 gcatccttca tcaccggctg cgattatccg ctcgatggcg gttggaccaa tctccggggc
     1261 tgatcacatg agggcgcgcg cgtgaccatc gattcgcatc aacatttctg gcgcttcgat
     1321 cccgtgcgcg acgcctggat cacgcccaaa atggcggtgc tgcgccgcga ctacatgccc
     1381 gccgatctcg cgcccgaact cgccgcccaa tccatcgacg ccaccattgc cgtgcaggcc
     1441 gatcaatccg agaacgaaac gcaattcctg ctcgatctcg ccgcgcttca tccattcatc
     1501 gcgggcgtcg tcggatgggt cgatctgctt tccccagagc ttccgcgccg cctcgaacat
     1561 ttctcccaat tcaaaaagct gtgcggcttc cgccacatcg cacagtctga gcccgacgac
     1621 tttctactgc gcgacgactt tcaacgcggc gttgcccgcc tcgccgattt cggcttcacc
     1681 tacgacgtcc tcatctacgc ccgtcaattg cccgccgccg ctgaattcgt ccgccgcttc
     1741 ccccaccagc cgttcgtgat cgatcacatt gccaagcccg atgtgcgcgc gcgcgaaatc
     1801 gatcgctggg aatcgcatat gcgcaccatc gccgcgcacc cgctcaccat gtgcaaactt
     1861 tccggcatga tcaccgaagc cgattggcgc gcgtggacgc ccgaaacctt ccgtccctat
     1921 ctggacatcg tctgggatgc ctttggtccc gaccgcctga tgttcggttc cgattggccc
     1981 gtttgcctgc tggccggcac ttatgctcaa gtgaagaatc tgatggagga cttccttcgg
     2041 gcgcgccccg ccgcgcaacg agaagccatt ttcggcgcca acgccgctcg cttttatagc
     2101 ctgaaaacac agcatggatt tacagcttag aaacaaggtc atcatcgtca ccggcggcgc
     2161 cagcggcatc ggcgcggcga ttgttgcagg agcctcccgc gaaggcgcag tcgccgtcat
     2221 cgtcgatagg aacgtcgaaa acgcgcttca ggtggagtgc gaccttcgca agtccggcgc
     2281 gcaggctcac gccctcttcg ccgatctcac gtccgccgaa aactgccgcc gcgtaattga
     2341 cgaaacgctc gaacgctgcg gccgcatcga cgcgctggta aacaatgcag gcttcaacga
     2401 tgccgtcggc ctggaaaacg gcaaccccga cgaatacatc aagtcgctcg agcgcaacct
     2461 gttccactat tacaatctcg cccacttcgc gctgccctcg ctgaagcagg cgcgcggcgc
     2521 catcgtcaac atcgcgtcca aaaccgctgt caccgggcag ggtggcacgt ccggctacgc
     2581 ttcctccaag ggcgccatcc tggcccttac ac
//
LOCUS       DASZMC010000248         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_388068,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000248 DASZMC010000000
VERSION     DASZMC010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388068"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1313)
                     /locus_tag="VG274_05025"
     CDS             complement(255..1313)
                     /locus_tag="VG274_05025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWE06049.1"
                     /translation="MATRVERLTYAAAQTARVAFYGAHYLVARVVAHDAFAEADAPRR
                     KFPSLRATLKGMRALFAADLNNAEQGLYPMPLDLADEMRRARASLLYLADIPRVAERR
                     RRHGHDEVDADADRLPRYYRQNFHFQTDGYLSDDSARLYDFQVEALFAGTADAMRRRA
                     YVPIARFIDGRDPGRMTLLDIGAGTGRFLSFVKKAQPRLKAVALDLSEPYLERARRAL
                     SKHRSVKFLAASAEDVPLDDASVDAAVSIFVFHELPPRVRTRVAREIARVLKPGGIFV
                     LADTIQYGDISDCDGLIDAFPTLLHEPYYESFARSDLGALFGAAGLKLIDTDIAYLTK
                     IWVFEKKAGRKPQGRKRS"
     gene            complement(1315..>1718)
                     /locus_tag="VG274_05030"
     CDS             complement(1315..>1718)
                     /locus_tag="VG274_05030"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06050.1"
                     /translation="TFTLPLAGQARVHAVDSDAAAIAALLAAARKTQKLKPVTAEVRD
                     LFKHPLSASELDRYDAAVLDPPRAGAAALAKTLAASKLTRAAYVSCNPESFARDACIL
                     GGSGWRIESVQPVDQFLWSSHIELVARLGRH"
BASE COUNT          338 a          530 c          543 g          307 t
ORIGIN      
        1 cgtgccaatc gcccgcaacg acaatcgccg cccaatcgga gaaaccgact tgcgccagaa
       61 cgggctgcgc aagcagtgcg aacagaatga cgaatgcggc gcagcagacc tttcctcccg
      121 cgcgcacgac tagcgtgaac acaaatgttt tcataaatgc ggccgacgaa gtcggcacaa
      181 ttgtcgaggt gagcggcgtg cactcggatt tcctccccga tcgtggggga ggaaaatgga
      241 gcgtgagctt acgttcaact tcgtttcctg ccttgcggtt tccgtcctgc tttcttttcg
      301 aacacccaaa tcttggtcaa atacgcgatg tcggtgtcga tcagcttcaa tcctgccgcg
      361 ccgaacaggg cgccgaggtc gctgcgtgcg aagctctcgt agtatggctc gtgcagaaga
      421 gtcgggaatg cgtcgatcaa tccgtcgcaa tcggagatgt ctccgtactg gatcgtgtcg
      481 gccaaaacga aaataccgcc tggtttcagc acgcgcgcga tctcgcgcgc cacacgggtt
      541 cgaacccgag gcggcagctc gtgaaacacg aagatgctga cggcggcatc cacgctcgcg
      601 tcatccaacg gcacatcttc ggcggaggcc gcgaggaatt tcacgctgcg atgtttcgaa
      661 agcgcgcggc gcgcccgttc aagatagggt tccgacagat ccagtgcgac cgccttcaac
      721 cgcggttgcg cttttttcac gaatgacaga aatctcccgg tccccgcgcc gatgtcgagc
      781 agcgtcattc gaccaggatc gcggccgtcg ataaaccttg caatcggcac ataggcacgc
      841 cgccgcatcg catcggcggt gccggcaaac agcgcctcga cctggaaatc gtagagtcgc
      901 gccgaatcat cgctcaaata tccgtccgtc tggaagtgaa aattctggcg gtagtagcgc
      961 ggcagacgat cggcatccgc atcgacctca tcatggccgt ggcggcggcg tctctcggcg
     1021 acgcggggaa tatctgcgag atagaggagg ctcgcccggg cgcgacgcat ctcgtcggcc
     1081 agatcgagcg gcatcggata gagaccctgc tcggcattgt tcagatccgc ggcgaagagc
     1141 gcccgcatgc ccttgagggt tgcgcggagc gacgggaatt tgcggcgcgg cgcatcggct
     1201 tcggcgaaag cgtcgtgggc gacgacgcgc gccaccagat agtgcgcgcc atagaaggcc
     1261 acacgcgccg tctgcgcggc cgcgtaggtc agtctctcca cccttgtcgc catctcagtg
     1321 ccgcccgagc cgggccacga gctcaatatg ggatgaccac aggaattggt ccaccggttg
     1381 aaccgattcg atccgccagc cactcccgcc gagaatgcag gcgtcgcgcg caaaactctc
     1441 cggattgcag gacacatagg cggcccgggt cagtttcgac gcggcgagcg ttttggcaag
     1501 cgcggccgct ccggcacgcg gcgggtcgag gacggccgca tcgtagcgat caagctcgct
     1561 ggcggagaga gggtgcttga atagatcgcg gacctcggcg gtcaccggct tcagcttttg
     1621 tgttttgcgc gctgcggcca atagcgccgc aatggcggcg gcgtcggaat cgacggcatg
     1681 gacgcgcgcc tgtccggcga gcggcagtgt gaatgttc
//
LOCUS       DASZMC010000249         3611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4775789,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000249 DASZMC010000000
VERSION     DASZMC010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4775789"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VG274_05035"
     CDS             complement(<1..329)
                     /locus_tag="VG274_05035"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWE06051.1"
                     /translation="MKTTYEIDPAHSSAQFVVRHMMITNVRGGFSSVKGTVVIDSDDP
                     SASTVEATIDVSSIATLDTQRDTHLKSADFLDAEHYPTITFKSKKVEKAGDDEYKLTG
                     DLTIHGVT"
     gene            537..1568
                     /locus_tag="VG274_05040"
     CDS             537..1568
                     /locus_tag="VG274_05040"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWE06052.1"
                     /translation="MAGLPGMPPVEDPKDIYAADHAGNLADAVKNFPARVYVPNSGSN
                     TVDVIDPATFKIVEHFPVGRQPQHVVPSWDLKTLWVLNDLGDSVTKIDPATGKKGETL
                     PVRDPYNMYFTPDGKYAIVVAEAKQALDFRDAQTMKLVDTINVDCKGIDHMDFTTDGR
                     FLIASCEFAGQALKVDVANRKIVGTLKLGAGGMPQDVKTAPDGKTFYVADMMANGIYL
                     IEPDSFKKIGFIATGKGTHGLYVSRDSKVLYISNRGEGSVTLLDLATQKIMTKWQIPH
                     GGSPDMGGVSADGKVLWLSGRYNAEVYAFDTTDGHLLARIKVGKGPHGLCVYPQPGRY
                     SLGHTGVFR"
     gene            1604..2359
                     /locus_tag="VG274_05045"
     CDS             1604..2359
                     /locus_tag="VG274_05045"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWE06053.1"
                     /translation="MKLTRRSFGRAASALAASIMARAQTPPDAAPNRLTATPAAGGSD
                     LKSGMLPLGFRTERDALLYVPESSLKLDKAALVISLHGASRSSDRGIEVLRAQADEFG
                     FLLLAPASSRVTWDIINGAGGLDAKFINRAMARVYEMRTIDPARVAMAGFSDGASASL
                     SLGLANGDLFAAVFGFSPGFIVDLPRAGKPSVFISHGTIDNVLPIDECSRRIVPQLRQ
                     QGYQVTYREFEGKHTLPPEIASEAMRWFMGLHA"
     gene            2335..3609
                     /locus_tag="VG274_05050"
     CDS             2335..3609
                     /locus_tag="VG274_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE06054.1"
                     /translation="MVHGTARVTEQKSSFRWLTALTTNERRVFTACIGGWALDAMNVQ
                     LYSFAIPALIAVWGLTRSQAGVLGTAALLISAAGGWVAGWFADRYGRVLTLQIAILTF
                     AVFTFLSGLAQNYTQLFIARALLGFGFGGEWAAGAVLLGEVIRAEHRGKALGIMQAGW
                     AIGWAAAALLYALFFSIMPAATAWRALFLVGLAPALLVFYIRRYVDEPEVYLTAKARI
                     ANTGDKPSLLEIFRAPLARVTFLGGVLGTGAQGGYYAVTTWLPTYLRTERGLSVLDSA
                     GYLVVLIAGSFAGYLSGGFLADRIGRRLGFLVFAIGAGAVVITYTMIPFGNTAMLALG
                     FPLGFFASGVFSGMGAFLTEHFPTRVRGVGQGFTYNIGRAAGALFPTLVGVLSTRMPL
                     GEAIGLFAGIAYATMAVAAFLLPETKGKVLAA"
BASE COUNT          680 a         1143 c         1042 g          746 t
ORIGIN      
        1 gtgacgccgt gaatcgtgag atcgccggtt agcttgtact cgtcgtcacc cgccttctca
       61 acctttttgc tcttgaatgt gatcgtggga tagtgctccg cgtccagaaa atcggcgctc
      121 ttgaggtgag tatcgcgctg cgtatcgagc gtcgcgatgc tgctcacatc gatggtcgct
      181 tctactgtcg atgcactcgg atcatccgag tcgatcacaa ccgtaccttt cacgctgcta
      241 aacccgccgc gcacgttcgt aatcatcatg tggcgcacca caaactgcgc gctcgagtgc
      301 gctggatcga tttcgtaagt agtcttcatg gctctcctta tcagtggatc ggactctcaa
      361 ttaaatacca cgcccaggat tctggtcatt ggcgatacta aaatcaaatg aacagaagtg
      421 cttttttcgt cataacgacg gctttatcat tccttactct ctcagggtgc tcgggtcaga
      481 aaacagacag tgaaacgaag gcgcaagcgg cccccgccca agccgcggga actcaggtgg
      541 ccggtcttcc cggcatgccc cctgtcgaag accccaaaga tatttacgcg gcggatcacg
      601 ccggcaatct tgcagacgcc gtcaaaaatt tccctgcccg cgtttacgtt ccgaacagcg
      661 gcagcaacac cgtcgacgtg attgatcccg cgacgttcaa aatcgtggag catttccccg
      721 tcggcaggca accgcagcac gtcgtgcctt catgggatct caagacgctt tgggtattga
      781 acgatctcgg cgacagtgtc acgaaaatcg accccgccac cggcaaaaaa ggcgagacgc
      841 tgcccgtgcg cgatccctac aacatgtatt tcacgccgga tggcaagtac gcgatcgtcg
      901 tggcggaagc gaagcaggcc ctcgatttcc gcgatgcgca aacgatgaag ctggtggaca
      961 cgatcaacgt ggattgcaag ggcatcgacc acatggattt cacgacggat ggccgcttcc
     1021 tcatcgcaag ctgtgaattc gccggacagg cgctgaaggt cgatgtggcg aatcgcaaga
     1081 tcgtcggaac cctcaagctc ggcgcaggcg gcatgcctca ggatgttaag acggccccgg
     1141 acggcaagac gttttacgtc gctgacatga tggccaatgg catttacctg atcgagcccg
     1201 atagcttcaa aaagatcggc ttcatcgcca caggcaaagg aacgcacggc ctctacgtca
     1261 gccgcgattc gaaggttctc tatatctcaa accgtggcga aggcagcgtc acgctgctgg
     1321 atctcgccac tcagaagatc atgacgaagt ggcagatacc gcatggcggc agccccgata
     1381 tgggcggcgt ctcggccgat ggcaaggtgc tttggctctc gggccgctat aacgcggaag
     1441 tgtacgcctt cgacacgaca gacggccacc tgctcgcgcg catcaaggtc ggcaagggtc
     1501 cgcacgggtt gtgcgtctat ccgcagccgg gccgctactc cctgggccac acaggcgtct
     1561 tccggtaacg gccttccggt cggttccggt aacatggttc gacatgaagc tcacgcgccg
     1621 ttcgttcggc agagccgcgt cggccctcgc agccagcatc atggcgcgcg cccagacacc
     1681 acccgacgcg gcgcccaatc gcctgacggc aacacctgcc gccggcggga gcgatctcaa
     1741 atccggcatg cttccgcttg gctttcgcac cgagcgcgac gcgctgctct atgtcccaga
     1801 gtcttccctg aagctcgaca aagcggcgct cgtgatctcg cttcatggcg ccagccgcag
     1861 ttccgaccgc ggcattgaag tgctccgcgc gcaagcggac gaattcggct tcctgttgct
     1921 cgcgcccgcg tcctcacgcg tcacatggga catcatcaac ggcgcaggcg gtctcgacgc
     1981 aaagttcatc aaccgcgcca tggcacgcgt ctatgaaatg cgcaccattg atccagcgcg
     2041 cgtcgccatg gccggattct cagacggcgc gtccgcatcg ctttcgctcg gactcgcgaa
     2101 cggcgatctc ttcgccgccg tcttcggctt ctctccagga tttatcgtcg atctcccgcg
     2161 cgccggcaag ccatctgtgt tcatctcgca cgggaccatt gataacgtgc tgccaatcga
     2221 cgaatgcagc cgccgcatcg tgccgcagct tcggcagcaa ggctaccagg tcacataccg
     2281 cgaattcgag ggcaaacaca cactgccacc ggaaatcgcg tccgaggcga tgcgatggtt
     2341 catgggactg cacgcgtaac ggagcagaaa tcatcgttcc gctggctgac cgcgctcacc
     2401 acaaacgagc gccgcgtctt tacggcttgc atcggcggct gggctctcga tgcgatgaac
     2461 gttcagctct acagcttcgc gatccctgca ctgatcgcgg tctggggctt gacgcgcagc
     2521 caggcaggcg tgctcggcac cgctgcattg ctgatttcgg ccgcgggcgg atgggttgca
     2581 ggatggtttg cggaccgata cggccgcgtc ctgacgttac aaatcgcgat cctcaccttc
     2641 gctgtattta cattcctctc agggctcgcg cagaactaca cgcagctctt catcgcacgc
     2701 gcgttgctcg gtttcggatt cggcggcgaa tgggccgcgg gagccgtgct gctcggcgaa
     2761 gtcattcgcg cggaacatcg cggcaaagcg ctgggcatca tgcaggcagg ttgggcaatc
     2821 ggatgggccg cggccgcgct cctctacgct ttgttcttct cgatcatgcc cgcagccaca
     2881 gcgtggcgcg cgctctttct tgtcggactc gcgccggcgc tactggtctt ctatatcagg
     2941 cgctacgtag acgaacccga ggtgtacctc accgcgaagg cgcgtatcgc aaacaccgga
     3001 gacaagccgt cgctgctcga aatcttccgc gcgccgctgg ccagggtgac ctttctgggc
     3061 ggcgttctcg gaaccggcgc gcaaggcgga tactacgccg ttaccacctg gctcccaacc
     3121 tacctgcgga ccgaacgcgg actttccgtt ctcgattccg ccggttatct cgtcgtattg
     3181 atcgcaggtt ctttcgccgg ctatctctcg ggaggctttc tcgcggaccg tattgggcgc
     3241 cggctcggct ttctggtatt tgccatcgga gcgggcgctg tcgtcatcac ctacaccatg
     3301 attcccttcg gaaacaccgc catgcttgcg ctcggatttc cgctgggctt cttcgcctcg
     3361 ggcgtgttca gtggcatggg cgcgtttctt acagagcact tccctacccg ggttcgcggt
     3421 gtcggtcagg gtttcacata caacatcggc cgcgcggcag gcgcgctgtt cccgactctg
     3481 gttggagtgc tgtcaacgcg catgccgctc ggtgaagcga tcggcctgtt tgccggcatc
     3541 gcgtatgcca cgatggcggt agcggcattc ctgctgccgg aaacgaaggg gaaagtactc
     3601 gcggcatgaa g
//
LOCUS       DASZMC010000250         6442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_920337,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000250 DASZMC010000000
VERSION     DASZMC010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920337"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1768)
                     /locus_tag="VG274_05055"
     CDS             complement(<1..1768)
                     /locus_tag="VG274_05055"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HWE06055.1"
                     /translation="MKSVRLARIGATLLCATLSSSFSASAPGAKHDPFKMLAAIDGTS
                     IANPPPGDWLSNGRDYREQRFSPLAAINTANVKDLGVAWEFRTHSVRALEATPIVANG
                     VMFVSSAWSKVFALDAKTGALLWSYDPQVPGAWARYACCDVGNRGVALWKGAVYVGTL
                     DGRLVKLDTATGKPVWDVSTIDRSKAYTITGAPRIVKGLVIIGNGGAEYDTRGYVGAY
                     DAQSGKLRWRFYVVPGDPARPPENPAMAAALKTWSRGKFEWWKMGGGGAPWDSMAFDP
                     TLDLLYVGTGNGAPWNRNMRSPAGGDNLYLSSILALRPETGQLVWYYQTTPGDTWDFD
                     STSHMILADLPIGGVTRKVIIQAPKNGFFYVLDRETGKLISAKPYAAVNWAKGIDQTT
                     GRPIENPAARYGIGTAVVMPSETGGHNWQPMAFDPATGLVYIPAMDGTALFVPQKPFV
                     YEPRAWNTGNDFAAITDAILGAVKTGKPPPPALGYIKAWDPVAQKEVWHVPMAGSWNG
                     GLLATAGGLVFGGGADGNFSAYDARSGAKLWSIDLTTGILAPPMSYAIDGEQYIAVLA
                     GWGGAGGLSAFKDPHAALSKYGI"
     gene            2054..3295
                     /locus_tag="VG274_05060"
     CDS             2054..3295
                     /locus_tag="VG274_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE06056.1"
                     /translation="MNRPAFLSVRHGAEETAFAILFAVSFAHLLNDMMQALLPAIYPT
                     LKTDYHLDYAEIGLVTLAFQLTASLLQPLVGLVADRRPMPYSLATGMVFTLAGLLMLS
                     VAHSYAMILFSAACVGLGSSVFHPESSRVARMAAGRRYGLAQSIFQLGGNIGSALGPF
                     TAAFIVLAWGQRSIAWYSLAALAAMFILWNVGVWYKTHGLTRLKAARHAAQHATLSPG
                     EVRKSIAILLMLIFSKYIYLTSINSYFTFYLMQKFHVSVKSAQLHLFIFLAAVAAGTF
                     LGGPMGDRLGRKYVIWFSILGALPFTLALPFANLFWTTALSIAIGIILASAFSAIVVY
                     GQELVPGRVGMVAGRFFGFSFGMAAIGAAVLGEIADLTSITFIYRVCAFLPLLGLFAM
                     FLPNVETSAQKKLPRDEIIPE"
     gene            complement(3454..3789)
                     /gene="grxD"
                     /locus_tag="VG274_05065"
     CDS             complement(3454..3789)
                     /gene="grxD"
                     /locus_tag="VG274_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HWE06057.1"
                     /translation="MTGTVSERIASDVGSNDVVLFMKGTPVFPQCGFSAAVVQILTDL
                     GVKFKSFDVLKDPELRQGVKEFSNWPTVPQLYVKGEFVGGCDIIREMHESNELEALLK
                     QHGVALANA"
     gene            complement(3855..4088)
                     /locus_tag="VG274_05070"
     CDS             complement(3855..4088)
                     /locus_tag="VG274_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HWE06058.1"
                     /translation="MAMSASEIEKLIREAFPDAVVTITDLAGDNDHYAAMVITSAFKG
                     KTRVQQHQMVYDALKGRMGGVLHALSLQTVAKD"
     gene            complement(4100..6298)
                     /gene="purL"
                     /locus_tag="VG274_05075"
     CDS             complement(4100..6298)
                     /gene="purL"
                     /locus_tag="VG274_05075"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HWE06059.1"
                     /translation="MTEITPQLVREHGLSPAEYARIVELLGRAPNFVELGIFSVMWSE
                     HCSYKSTRAHLRRLPTKVPWVLQGPGENAGVIDIGDGDVAVFKMESHNHPSFIEPYQG
                     AATGVGGIMRDVFTMGARPVAMMNALRFGAPEHEKTRHLVSGVVAGIGGYGNCMGVPT
                     VGGEVNFHSSYNGNILVNAMCVGIARADKIFKSAAKGAGNPVVYIGSKTGRDGIHGAT
                     MASTEFSADSQEKRPTVQVGDPFTEKLLLEACLELMATDAIIAIQDMGAAGLTSSSAE
                     MASKGGSGIVLDLDRVPQREEKMTAYEMLLSESQERMLAVLKPGREEEAERIFRKWEL
                     DFAVIGTTTDTSRFVVRHEGRTVADIPAQALSEEAPVYQRPFEKRSAQVQEPSFDRAN
                     PVIDSLVKLIGSPDLCSRRWIWEQYDWSVMADTVQKPGGDAAVVRVHGADKALAITTD
                     VTPRYCRADPFEGAKQAVAEAWRNLSAVGAKPLAVTDNLNFGNPEKPDIMGEIASCID
                     GIAEACRALDFPVVSGNCSLYNETNGEAILPTPAIGAVGLINDVRRMATPAFKRENDA
                     IVLIGETKGHLGQSLYLREIEGKEIGGAPGVDPAAERRHGDFVRGLIESGKLDTVHDV
                     SDGGLLIALAEMAMAGGIGARIASSERSHAIPFCFGEDQGRYLVALPDADPLLQEAAN
                     VGIPAVLLGQTGGDRIAVAGVGEVGLAALKRAHEAWFPGYMSNAEPPPTN"
BASE COUNT         1042 a         2135 c         2030 g         1235 t
ORIGIN      
        1 tgatcccgta tttgctgagc gcagcatggg gatccttgaa cgcggaaagt ccgcccgcac
       61 cgccccagcc tgcaagcacc gcaatatatt gttcgccgtc gatggcgtag ctcatcggcg
      121 gcgccagtat tccggtcgtc agatcgatcg accacaattt cgcgccggac cttgcatcgt
      181 aggcggagaa attgccgtcc gcgccgccgc cgaacacgag accgcccgcc gtcgccagaa
      241 gcccgccgtt ccagctgccc gccatcggca cgtgccacac ttccttctgc gccacgggat
      301 cccatgcctt gatgtagcca agcgcgggcg gcggcggctt gccggtcttg accgctccca
      361 gaatggcgtc ggtgatggcg gcgaagtcgt tgccggtgtt ccaggcgcgc ggttcgtaga
      421 cgaaaggctt ttgcggcacg aagagtgcag ttccgtccat cgccggaatg tagacaaggc
      481 cggtcgcggg atcgaaggcc atgggctgcc agttgtggcc ccccgtttcg gacggcatca
      541 cgaccgccgt cccgatgccg taacgcgcag cgggattttc gatcgggcgc cccgtcgtct
      601 gatcgatgcc cttcgcccag ttcaccgccg cgtaaggctt cgccgaaatg agctttccgg
      661 tttcgcggtc gagcacgtag aagaaaccgt ttttcggcgc ctggatgatg accttgcgcg
      721 tcacgccgcc gatcggaaga tctgcgagga tcatgtgcga ggtagaatcg aaatcccagg
      781 tgtcgccggg cgtcgtctgg tagtaccaaa cgagttggcc cgtctccggc cgcagcgcca
      841 ggatcgagga gagatagaga ttgtcgcccc cggccggcga acgcatgttg cggttccagg
      901 gcgcgccgtt gcccgtgccg acatagagca gatcgagcgt gggatcgaag gccatcgaat
      961 cccacggcgc gccgccgccg cccatcttcc accactcgaa tttgccgcgc gaccaggttt
     1021 tcagcgccgc cgccatcgcc ggattttcgg gcggacgcgc aggatctccg ggcacgacgt
     1081 aaaagcgcca gcgcagtttt ccgctctgcg cgtcataggc gccgacatag ccgcgcgtgt
     1141 cgtattccgc gccgccattg ccgatgatga cgaggccttt cacgatccgc ggcgcgccgg
     1201 tgatggtgta ggccttgctg cggtcgattg tgctgacgtc ccagaccggc ttgccggtgg
     1261 cggtatcgag cttcaccagc ctgccatcga gcgtgcccac atagaccgcg cccttccaga
     1321 gcgcgacgcc gcggttgccc acgtcgcagc aggcgtagcg cgcccaggcg cctggaacct
     1381 gcgggtcgta gctccacagc agcgcgcccg tcttcgcatc gagcgcgaac accttgctcc
     1441 acgcagagga cacgaacatc acgccgttgg ccacgatcgg ggtcgcttcg agggcgcgga
     1501 cggaatgggt gcggaactcc catgcgacgc cgaggtcctt cacattggct gtgttgatcg
     1561 cggcgagcgg agagaagcgc tgctcgcgat agtcgcggcc gttcgacagc cagtcgccgg
     1621 ggggcggatt cgcgatgctc gtgccgtcga ttgcagcaag cattttgaat ggatcgtgct
     1681 tggcgcctgg cgcagacgct gagaaactcg atgagagtgt agcgcaaagc agcgtcgcgc
     1741 cgatccgcgc aagtcgaaca cttttcatgt ttcctgcccc gccgaatccg acgcgatcag
     1801 gttagggcgg ttttgtccgc ggcgacaaca cgcgcgttga attccgatcc gcggcgttcc
     1861 ccctccgcct cttcggggca cccccgcgcg ctcgtcgcgc gtgggggtgg cgcgaagcgc
     1921 cggagggtgc agggcggtcc agcgcgaagg cacttcgatc ctggctgtca tgcgtcggac
     1981 tcgtgcagcg gatgtggcgt gacgggtccc ggccatgtat gccggtccct cacatgtgcg
     2041 gcgcaactag cagatgaacc ggcccgcttt cctgtccgtc cggcacggcg cggaagagac
     2101 ggcgttcgcc attctctttg cggtcagctt cgcgcatctg ctcaacgaca tgatgcaggc
     2161 gctattgccg gcgatctatc cgacgctcaa gaccgattac catctcgact atgcggaaat
     2221 cggcttggtg acgctcgcct tccagctcac ggcctcgctc ctgcagccgc ttgtggggct
     2281 cgtcgcggac cgacggccga tgccctattc gctcgccacc ggcatggtgt tcacgctcgc
     2341 cggactgctg atgctctcgg tcgcgcactc ctacgcgatg atccttttct ccgcggcgtg
     2401 cgtggggctc ggctcgtcgg tgttccatcc cgaatcctcg cgcgtcgcgc gcatggcggc
     2461 gggccgcaga tatggcctgg cgcaatcgat ctttcagctc ggtggcaata tcggctcggc
     2521 gctcggcccc ttcaccgccg ccttcatcgt actcgcctgg ggccagcgga gcatcgcgtg
     2581 gtattcgctg gccgcgctcg ccgcgatgtt catcctctgg aatgtgggtg tgtggtacaa
     2641 gacgcacggc ctcacgcggc tcaaagccgc gcggcatgcg gcgcagcacg cgacgctgtc
     2701 gccgggcgag gtgcgaaagt cgatcgcgat cctgctcatg ctgatcttct cgaaatacat
     2761 ctacctgacc agcatcaaca gctatttcac cttctatctg atgcagaaat tccacgtctc
     2821 ggtgaagagc gcgcagttgc acttgttcat cttcctcgcg gcggtcgcgg ccggcacgtt
     2881 tctgggcggt ccgatgggag accgattggg acgcaaatac gtcatctggt tctcgatcct
     2941 gggcgcgctg ccgttcacgc tggcgctccc cttcgccaat ttgttctgga cgacggcgct
     3001 gagcatcgcc attgggatca tcctcgcctc cgccttttcg gcgatcgtcg tctacgggca
     3061 ggagctggtg cccgggcgcg tcggcatggt cgcgggcagg ttcttcggat tttcgttcgg
     3121 catggcggcg atcggcgccg cggtgctggg cgagatcgcc gacctgacca gcatcacctt
     3181 catctaccgc gtctgcgcct tcctgcccct gctcggcctc ttcgcgatgt tcctgccgaa
     3241 cgtcgaaacc agcgcgcaga agaagctccc ccgcgacgag attattccgg agtaagcgcg
     3301 gcggccccct ccggcccttt gggccacctc ccccaacgct tcgcgtcggg ggaggaaagc
     3361 gtgacccgat gcgctccact ttcctccccc gcgcgcttgc gcgtggggga ggtgccgacc
     3421 ccggatcaaa tccgggggag gcggagggga actttacgcg ttcgccagcg cgacgccgtg
     3481 ttgtttgagc agggcttcga gctcgttcga ttcgtgcatc tcgcggatga tgtcgcagcc
     3541 gccgacgaat tcgcctttga cgtagagctg cggcacggtc ggccagttgg agaattcctt
     3601 gaccccctgc cggagttcgg gatccttgag cacgtcgaag cttttgaact tcacgcccag
     3661 atcggtgagg atctgcacga ccgcggcgga gaacccgcat tgcggaaaca ccggcgtgcc
     3721 cttcatgaac agcacgacgt cgtttgatcc gacatcgctt gcgattcgtt ccgagacggt
     3781 tccggtcatg gctacctctg gctggcgatg ctcgaggact tagggtccgg caccgcgatg
     3841 gtcaagggag gcgctcaatc cttggcgacg gtctgcagcg acagggcgtg aagcacgccg
     3901 cccatccggc ccttcagcgc atcgtacacc atttgatgct gctggactcg cgtcttgccc
     3961 ttgaaggcgc tggtgatgac catggccgca taatgatcgt tgtcgccggc gagatcggtg
     4021 atcgtcacca ccgcatccgg aaaagcctcc cggatcagtt tctcgatctc gctcgcggac
     4081 atcgccatca ggaattccct cagttcgtcg gcggcggttc ggcattggac atgtagccgg
     4141 ggaaccaggc ttcgtgcgcg cgcttgaggg cggccaagcc cacttcgccc acccccgcga
     4201 cggcaattct atctcccccg gtctgtccca gcaaaacagc gggaatcccg acattcgctg
     4261 cctcttgcag caggggatcc gcgtcaggca gggcaacgag ataccggccc tgatcctcgc
     4321 caaagcagaa tggaatcgcg tggcttcttt cgctgctcgc aatccgcgcg ccgattccgc
     4381 cggccatcgc catctccgcc agcgcgatca gcagtccgcc gtccgaaaca tcgtggacgg
     4441 tgtccagctt tccggattcg atcagtccac ggacgaaatc tccatggcgt ctctccgcag
     4501 cgggatcgac cccaggcgcg ccgccaattt cttttccctc gatctcgcgc agatagagcg
     4561 attggccgag atgccctttc gtttctccga tcaggacaat cgcgtcgttc tcgcgcttga
     4621 acgccggcgt tgccatgcga cggacgtcgt tgatgaggcc gacggcgccg atcgcgggcg
     4681 tcggcaatat cgcctcgcca ttggtctcgt tgtagagcga gcagtttccc gacacgacgg
     4741 ggaagtcgag tgcccggcag gcttccgcga tgccgtcgat gcatgatgcg atctcgccca
     4801 tgatgtcggg cttctcggga ttgccgaaat tcagattgtc ggtgacggcg agcggcttcg
     4861 cgccaacggc gctcaggttg cgccacgctt cggcgaccgc ctgcttcgcg ccttcgaagg
     4921 gatcggcgcg gcaatagcgc ggcgtcacat ccgtggtgat ggcgagcgcc ttgtcggcgc
     4981 catgcacgcg caccaccgcg gcatcgcctc ccggcttctg caccgtatcc gccatcacgc
     5041 tccaatcgta ttgctcccag atccagcgcc gcgagcagag atcgggcgaa ccgatgagct
     5101 tcacgagcga atcgatgacg gggttcgcgc gatcgaagga gggttcttgc acttgcgccg
     5161 agcgcttctc gaacggccgc tggtagaccg gcgcttcctc ggagagcgct tgcgcgggga
     5221 tgtcggcgac ggtccggcct tcgtgacgca ccacaaaccg gctggtatcc gttgtcgtgc
     5281 cgatgacggc gaaatcgagc tcccatttcc tgaaaatgcg ctccgcctct tcctcccgcc
     5341 ccggcttcag caccgcaagc atgcgttcct gagactccga gagcagcatc tcgtaggccg
     5401 tcatcttctc ttcgcgctgc ggaacgcgat cgagatcgag cacgatgccc gagccgccct
     5461 tcgacgccat ctcggccgac gacgaggtga ggcccgccgc gcccatgtcc tggatggcga
     5521 taatcgcatc cgtcgccatc agttcgaggc aggcttcgag cagcagcttc tcggtgaagg
     5581 gatcgcccac ctgcacggtc gggcgcttct cctgcgagtc cgcgctgaat tccgtggacg
     5641 ccatggtggc gccgtggatg ccgtcgcgtc ccgtcttgga gccgatatag acgacgggat
     5701 tgcccgcgcc tttcgccgcc gatttgaaga ttttgtcagc gcgcgcgatt cccacgcaca
     5761 tcgcgttgac gagaatgttg ccgttgtacg agctgtggaa attcacctcg ccgcccaccg
     5821 tcggcacgcc catgcaattg ccgtaaccgc cgatgccggc aacgacgccg gagacgaggt
     5881 ggcgcgtctt ctcgtgctcc ggcgcgccga agcgcagcgc gttcatcatc gccacgggcc
     5941 gcgcccccat ggtgaagacg tcgcgcatga tgccgcccac gccggtggcc gcgccctgat
     6001 agggctcgat gaaggaggga tggttgtggc tttccatctt gaacaccgcc acgtcgccat
     6061 cgccgatatc gatgacaccg gcattctcgc ccggcccctg cagcacccag ggcaccttcg
     6121 tcggcaggcg gcgcaaatgc gcgcgcgtcg atttgtagga acaatgctcg ctccacatca
     6181 ccgagaagat gccaagctcg acgaaattgg gcgcgcggcc gagcagctcg acgatgcggg
     6241 cgtattcggc gggcgaaagg ccgtgctcgc ggacgagttg gggcgtgatc tcggtcaaga
     6301 gtcccctccg cccgcttcac gggcgcctcc cccacgcgca agtgcgcggg ggaggaaata
     6361 ggagagctcg tgtctcgcaa ttcgctttcc tccctcgcgc gaagcgcggg ggaggtgccc
     6421 cgaaggggcg gagggggccg gc
//
LOCUS       DASZMC010000251         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1756494,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000251 DASZMC010000000
VERSION     DASZMC010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756494"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VG274_05080"
     CDS             <1..709
                     /locus_tag="VG274_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWE06060.1"
                     /translation="AALAFAHLRAEDLEEPVGAPAIEIGLELTSPRVEPTDLPPGPDT
                     EASVASPAIAEQKAEVKETELPKDVPSETDNPDRAVTPNDSSKPREDDAKPATVQTQA
                     STESEAVEATATPSSEDIPQAARSVAPAQGTGKSAQRLRATWQKELMAHLDRHKRYPA
                     QRSERSAEILVSFALDRKGHVLSTSIVKGSGDTAFDEAALAMLRRSDPVPAPPPAVAD
                     EGLSFTLPVIFRVKGHG"
     gene            717..839
                     /locus_tag="VG274_05085"
     CDS             717..839
                     /locus_tag="VG274_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06061.1"
                     /translation="MARLASWFHKSNFFQVFDAALVISVEFATDAEKLNDDIPQ"
     gene            820..1362
                     /locus_tag="VG274_05090"
     CDS             820..1362
                     /locus_tag="VG274_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06062.1"
                     /translation="MTISHSELISVLHAEKPAPDRAEKLQLYGRFIGDWDAKIITHAP
                     DGAAHERSGEIHFGWILEGRAIQDVWMTPRLAERPKAPPLPVAGNWFGTTIRVYDSMI
                     DAWHIYWIDPATNSYRQQIGRPRGHDIVQEGKTDSGALSRWSFTRITATSFHWLGESS
                     PASDGPWQLLVEVLAQRKSA"
     gene            1582..>2287
                     /locus_tag="VG274_05095"
     CDS             1582..>2287
                     /locus_tag="VG274_05095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE06063.1"
                     /translation="MAVLDFFVVNVAIPDLQRDLNASSAQIQPIVAGYALAYGSVLIV
                     GSRLGDIYGRRRMFVSGLALFTISSAACGLAPNAAVVVFARGAQGVSAALLSPQILAI
                     LSSAYQATAKARALNAYGLCMGLASVFGQVFGGLLIHVDLFGGDWRSCFLINLPIGLV
                     AIALALRIVPESSAPMRPRLDLTGMFVISLALLAATMPLIEGREQGWPFWSWLSLAAA
                     AILLVLFVVHENRVKIA"
BASE COUNT          411 a          678 c          752 g          446 t
ORIGIN      
        1 cgccgcgctg gcgtttgcgc atctgcgagc cgaggatctc gaggaaccgg tcggcgcgcc
       61 ggcgatcgag atcggcctgg aattgacgtc gccgcgcgtt gagccgaccg atttgccacc
      121 gggtccggac accgaggctt ccgtcgcttc gcccgcgatt gcggagcaaa aggcggaggt
      181 caaggaaacc gagctgccga aggacgtgcc gagcgaaacc gacaatcccg atcgggcggt
      241 gacaccgaac gattcgagca agcccaggga agacgacgcg aaacccgcca cggtgcaaac
      301 ccaggcctcg acggaatccg aagcggtaga ggcgaccgca acgcccagtt ccgaagacat
      361 cccgcaagcc gctcgctcgg tcgcgcccgc ccagggcacc gggaaaagcg cgcagcggct
      421 gcgtgcgacg tggcagaagg agctgatggc gcatctggac cggcacaagc gctatccggc
      481 gcagcgctcg gagaggagcg cggagattct ggtcagcttt gcgctcgacc ggaagggaca
      541 tgtgctctcg accagcatcg tcaagggatc gggcgacacg gcgttcgatg aggccgcgct
      601 ggcgatgctg cggcgatccg atccggtgcc agcgccgccg cctgccgtcg ccgacgaggg
      661 cttgagtttt accttgccgg tgattttccg ggtcaaggga cacggttgag ctttcgatgg
      721 cgcgtctagc gtcatggttt cacaaaagca atttcttcca agttttcgac gcggcacttg
      781 tgatctcggt cgagttcgca acagacgcgg agaagctcaa tgacgatatc ccacagtgaa
      841 ttgatttcgg ttctgcacgc ggaaaagccc gcaccggacc gggccgaaaa attgcagctt
      901 tatggaaggt tcatcggcga ttgggatgca aagatcataa ctcatgcgcc cgacggcgct
      961 gcgcatgaac gttcggggga gatccacttt ggctggatcc tcgaaggccg cgcgatccag
     1021 gacgtctgga tgacgcctcg actggcggaa cgcccgaagg cgccgcctct tcctgtcgcc
     1081 ggcaattggt ttggaacgac catcagggtc tacgactcca tgatcgatgc ctggcacatc
     1141 tattggatcg accccgcaac caattcctat cggcagcaaa tcggccgccc gcgcgggcac
     1201 gatatcgtgc aggagggcaa gaccgactcg ggcgcgctgt cgcgatggag ttttaccagg
     1261 atcacggcaa cttcgtttca ctggttggga gaatcgtccc cggcgagcga cggcccatgg
     1321 caattgctcg tggaggtgct ggcgcagcgc aagtcggctt aagggcggca tttttccgcg
     1381 atctcgagcg cgctggcggc cgttgcggaa atcttgctgc ggcgccgatt ccgttccata
     1441 cggcgctgaa aggaactggt cgtgagacag gccgaatcca tttgctcgaa acaatcttgc
     1501 ggttcgcact cgcggtccca ccgctcctcg acggataggc ccgattggct ggtcgtcgga
     1561 gttttgctga ccggcacgtt catggcggtg ctcgattttt tcgtcgtgaa cgtcgcaatt
     1621 ccggatttgc agcgcgacct gaatgccagc tccgcgcaaa tccagccgat cgttgcgggt
     1681 tacgcgctgg catatggatc ggtgctcatt gtcggaagcc gcctgggcga tatctacggg
     1741 cgtcggcgaa tgttcgtatc ggggctggcg ctgtttacga tatcctccgc cgcgtgtggc
     1801 ctggcgccga atgcagcggt ggtcgtgttc gcacggggcg cgcagggcgt atcggccgcg
     1861 ctgctgtctc cgcagatact tgccatcctc agttcggcat accaggcaac ggcaaaggcg
     1921 cgggcgctga atgcctacgg cctctgtatg gggctggcat ccgtgttcgg tcaggtcttt
     1981 ggtggccttc tcatccatgt cgacctgttc ggcggggact ggcggagctg cttcctgata
     2041 aatctgccga ttggcctggt cgcgattgcc cttgcattac ggatagtccc ggaatcatcg
     2101 gcgccgatgc gacctcggct cgacctgacg ggcatgttcg tgatttcgct ggcgctgctc
     2161 gcagcgacaa tgccgctgat cgaggggcgc gagcagggat ggccgttttg gtcatggctg
     2221 tcgctcgcgg cggctgcgat cctgctcgtg ctgttcgtgg tccacgaaaa ccgggtcaag
     2281 atagcgg
//
LOCUS       DASZMC010000252         6852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_97176,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000252 DASZMC010000000
VERSION     DASZMC010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97176"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VG274_05100"
     CDS             <1..307
                     /locus_tag="VG274_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06064.1"
                     /translation="PVSRQKESIMRLVPVTISAVALCASIAFGEATASADEARPSSCP
                     KLASEVNDALASNAQSANYEAAVKEKRYGLEFCANGLYRNGAEHYAEALKLLGASKG"
     gene            complement(279..923)
                     /gene="metW"
                     /locus_tag="VG274_05105"
     CDS             complement(279..923)
                     /gene="metW"
                     /locus_tag="VG274_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HWE06065.1"
                     /translation="MKILRPDLAAIAALIPPRARVLDVGCGEGDLLEHLAVGKQVDGR
                     GIELSQQNVNACVERGLSVVQGDADTDLADYPSAVFDIVILSQTIQAMRSPRAVLAQL
                     LRIGRHTVVSFPNFGHWRVRLELMFVGRMPQTPALGYSWHETPNIHLCTIADFIALAD
                     EVGARIEQAFALSGKGRTRPMRPDSWGPNFLAEGAILMLTAAAPALSPSTHPAI"
     gene            complement(1063..2238)
                     /locus_tag="VG274_05110"
     CDS             complement(1063..2238)
                     /locus_tag="VG274_05110"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWE06066.1"
                     /translation="MAEVANPRFLRPLAAPGEDAGQAIGFGSSQPLMLDAGRALSPFT
                     VAFKTYGTLNDDRSNLVLICHALTGDQFAASRHPVTGKPGWWDIMIGPGKPIDTSRYF
                     VLCPNVIGGCMGTTGPAETNPSTGKPYGLSFPLITVRDMVRAQAMLLDALGIEKILCV
                     LGGSMGGMQVLQWAASFPARVVSAVPIAAAARHSAQNIAFHEVGRQAIMADPDWKNGD
                     YALHGTRPAKGLAVARMAAHITYLSETALQNKFGRALQDRDSFSFAFGPDFQIESYLH
                     HQGITFVDRFDANSYLYITRAMDYFDLAAEYGGVLAEAFRGSQTRFCIISFTSDWLFP
                     TTENKKVAHALNAAAANVSFVEIATDRGHDSFLLDEPEMFAAVRGFLNSVAAHHGVA"
     gene            2523..3611
                     /gene="hisC"
                     /locus_tag="VG274_05115"
     CDS             2523..3611
                     /gene="hisC"
                     /locus_tag="VG274_05115"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWE06067.1"
                     /translation="MNLKPKPGILDIAPYVAGRAEAPDGAPAVKLSSNESALGLSPLA
                     RAAYEEARGNLSFYPEGSARLLRDALGELHHLDPDRIVCGNGSDELLSLLPRIYASGG
                     DEVVFTEHAFLVYRIGALASGAVPVAVPETDLRADVEKILAAVTPRTRLVFLANPNNP
                     TGSCLSGAEVRRLHAGLRPSVLLVIDAAYAEYVQRRDYEAGDALVKQFDNVVMTRTFS
                     KIYGLAGLRVGWAFCPASVADALNRVRGPFNVSVHAQRAAVAALRDREHVARSIAHNE
                     TWKRWLIESIRTSGLRVDDSEGNFVLVHFPPGAYSAGAADAFLTARGFILRPVTNYGL
                     PHCLRLTIGSEDANRGVAAAIADFMRPV"
     gene            3611..4549
                     /locus_tag="VG274_05120"
     CDS             3611..4549
                     /locus_tag="VG274_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HWE06068.1"
                     /translation="MTIDSKPLFGKVALIGIGLIGSSLAHAMRRAGLAAHIAGYSHRS
                     ETVEKARAIGFADSFHTALAPAVRSADLVVLAAPVGAFGELARELGPHLKRGAILSDV
                     GSVKMAVLRDVGPHVPDGVHFIPAHPIAGTEQSGPESGFAELFDGRWCILTPLPGADD
                     GAVATLKSFWQRCGSQVQIMDPKHHDLVLAITSHVPHLIAYNIVGTAADLETVTEGEV
                     IKYSAGGFRDFTRIAASDPVMWRDVFLNNREAVLEMLGRFNEDLSALQRAIRWGDGEM
                     LFNLFTRTRAIRRSIIDAGQDIGEPNFGRPRRKNPT"
     gene            complement(4830..5009)
                     /locus_tag="VG274_05125"
     CDS             complement(4830..5009)
                     /locus_tag="VG274_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06069.1"
                     /translation="MPNYEIRYLNSEGELTLIHKTQHVDDEAVDLAALRMLMGSGLAD
                     YEILRDDDATPTIKR"
     gene            complement(5154..6182)
                     /locus_tag="VG274_05130"
     CDS             complement(5154..6182)
                     /locus_tag="VG274_05130"
                     /inference="COORDINATES: protein motif:HMM:NF021423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HWE06070.1"
                     /translation="MSMRYSSRLFLYLPFVLLIVLAIAAMLRWWVVAGTFERWLLHAN
                     GREIAPGIRLRFASEEMGGFPFRVDTVLSGLALEANCARASVSWRAERFAIHSLIYGR
                     AQKIFEAAGAQTLSWTMPEGGARRLVFTPGSLRASAILSEGVLARFDLDLAGFGSREV
                     SGARAQLHLRRAPSRDTIDIAISAEALRLAPELQAEFGPEIPHMVLVGRIAPAAPFSP
                     FLSGRGPWCAALDKWRGSAGSFRLDRLDMAWGQTSAEGSGRLTLDDLHRPQGALQLKV
                     AQTDQTSTPPAREARLSRALGRLARATRSTGEVPLEVSAAFNNGAVTLRHAHQPYAES
                     AGSFDALY"
     gene            complement(6262..>6852)
                     /locus_tag="VG274_05135"
     CDS             complement(6262..>6852)
                     /locus_tag="VG274_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06071.1"
                     /translation="RGAVLEVKKAAKPVVDDGDSAPEARPETLPGRTYKLHWPESDHA
                     IYITVNDILVDDPRGGTRRRPFEVFINSKNMEAYAWTLALTRMISAVFRRGGDVSFVV
                     DELKAVFDPRGGQWMGGQYVPSLLAAIGDVIERHLVATGFMLPRDRLARPETAESIAV
                     AAGERRGRTCPRCGSPSFIRQENCDVCIACGFSRCG"
BASE COUNT         1264 a         2123 c         2216 g         1249 t
ORIGIN      
        1 gccggtgtca cgacaaaagg agtcgatcat gcgtcttgtg ccggtcacga tctcagcagt
       61 agctttgtgc gcgagcatcg cctttgggga ggcgacggcg agcgccgacg aagcccggcc
      121 gtcgagctgt ccgaagctcg cgagcgaagt caacgatgcc ctggcgagca atgcccaatc
      181 cgcgaactat gaggcggccg taaaggaaaa gcgctatggc ctcgagttct gcgccaacgg
      241 actctaccgg aatggcgccg agcactacgc ggaggcgctc aaattgctgg gtgcgtcgaa
      301 gggctgagcg caggcgccgc cgcggtcaac atcaagatcg cgccttcagc gagaaaattc
      361 ggaccccagg aatcgggccg catggggcgg gtgcggccct ttccgcttag cgcgaaggcc
      421 tgttcgattc gggcgccgac ttcgtcggcg agtgcgatga aatcggcgat cgtgcagaga
      481 tgaatatttg gcgtttcgtg ccagctgtat ccgagcgccg gtgtttgcgg catccggcct
      541 acgaacatca gttccaggcg gacgcgccaa tggccgaaat tcggaaacga gacgacggtg
      601 tggcggccga tgcgcaggag ctgtgcaaga accgcgcgag gcgagcgcat cgcctggatc
      661 gtctggctga ggattacgat gtcgaaaacc gctgaaggat agtcggcgag gtcggtgtcg
      721 gcatcgccct gcacgaccga caggcctcgc tcgacgcagg cattgacgtt ctgctgcgac
      781 agctcgattc cccttccatc gacctgtttc ccgaccgcga gatgctcaag cagatcgcct
      841 tcgccacaac ccacatcgag cacgcgcgcg cgcggcggaa tcagcgccgc gatggccgcc
      901 aagtcgggcc gcaggatttt catctgaaaa acccaggttt tttaacgcag aggacgcgga
      961 ggaagcgcgg aggacgtaga ggaagtaatt gcgcgcttcg cgcgggattt ttctgctgcg
     1021 tcctctgcga aacctccgcg ccctctgcgt tcaaagcgtt tttcacgcca cgccatggtg
     1081 agccgcgacc gaatttaaga aacctcgcac cgccgcaaac atttccggct cgtcgagaag
     1141 gaaggaatcg tggccgcggt cggtcgcgat ttcgacgaag ctgacattgg ccgcggcggc
     1201 gttcagcgcg tgcgcgacct tcttgttttc agtggtcgga aacagccagt cgctggtgaa
     1261 ggagatgatg cagaacctgg tctgcgatcc gcgaaacgct tccgcgagca cgccgccata
     1321 ttcggcggca aggtcgaaat aatccatcgc gcgggtgatg tagagataag agtttgcgtc
     1381 gaagcggtcg acgaaagtga tcccctggtg atggagatag ctttcgatct ggaagtccgg
     1441 tccgaaagcg aaggagaacg agtcgcgatc ctgcagggcg cggccgaatt tgttttgcag
     1501 cgcggtttcg gagaggtagg tgatatgggc ggccatgcgc gcgacggcga gtcccttggc
     1561 cgggcgcgtt ccgtggagtg catagtcccc attcttccag tccggatcgg ccatgatcgc
     1621 ctgtcgcccc acttcatgga acgcgatgtt ctgcgcggag tggcgggccg ccgcggcgat
     1681 cgggacggcg gagaccacgc gcgcgggaaa gctcgccgcc cattgcagca cctgcatgcc
     1741 gcccatcgaa ccgccgagca cgcagagaat tttctcgatg ccgagcgcgt cgagcagcat
     1801 cgcctgcgcg cgtaccatgt cgcgcacggt gatgagcgga aaggacaggc cgtagggttt
     1861 cccggtcgag ggattggtct ctgccggtcc cgttgttccc atgcagccgc cgatgacatt
     1921 gggacaaaga acgaagtagc ggctggtgtc gatcggcttc ccgggaccga tcatgatgtc
     1981 ccaccatccg ggtttgccgg tcacgggatg gcggcttgcc gcaaactggt cgccggtcag
     2041 cgcatggcag atcagcacca gattggatcg gtcgtcatta agcgtgccat aggtcttgaa
     2101 cgcgaccgta aagggcgaaa gcgcgcgtcc ggcgtcgagc atcagcggct gcgacgaccc
     2161 gaaaccgatc gcctgtcccg catcctcgcc cggcgccgcg agcggtcgca ggaaacgcgg
     2221 gttcgccact tccgccatga acggctccca tccttcgatc cgccttcgcc aaggcttcgg
     2281 cggacatgag agaagaacaa acccgccgga gcttcagcgt aggcgggcag gctcaggatg
     2341 aggccctcat ggtgagcttg tcgaaccatg tgggccattc ggcggatcac actcctgcca
     2401 tcccgctact aaggcgcggt ctgtctgtgt gtcaacgcga gaatgcggaa cgtgtttttg
     2461 atttcgcggc gcgtgccctc tatcaatgca ggttcgctgc cggctcacca aggcaccatg
     2521 acatgaacct caaaccgaag ccgggaatac tcgacattgc accgtatgtc gcggggcgcg
     2581 ccgaggcgcc ggacggcgct ccggccgtca agctttcgtc gaacgaatcg gctctgggac
     2641 tgagtccgct ggcacgcgca gcctatgaag aggcgcgggg gaatctgtcg ttctatccgg
     2701 aaggaagcgc gcgcctgctt cgcgacgctc tcggagagct tcatcatctc gatcccgatc
     2761 gaatcgtgtg cggcaacggt tccgacgaac tcctctccct gctcccgcgc atctacgctt
     2821 cgggcggcga cgaggtggtg ttcaccgagc acgcctttct tgtctaccgg atcggcgcgc
     2881 tcgccagcgg cgcggttcca gttgcggttc cggaaacaga cttgcgcgcg gacgtggaga
     2941 agattctcgc cgccgtcacg ccgcgaacac ggctcgtttt cctcgccaat ccgaacaatc
     3001 cgaccggcag ctgcctttcc ggtgcggaag ttcgccgcct ccatgccggc ttgcggcctt
     3061 cggtcctgct ggtgatcgat gccgcgtacg cggaatatgt gcagcggagg gattacgaag
     3121 ccggcgacgc gctggtgaag cagttcgaca acgtggtcat gacgcgcacc ttctcaaaaa
     3181 tttacggcct tgcgggtctg cgcgtcggtt gggcgttctg ccccgcatcc gtggcggacg
     3241 cgctcaatcg cgtgcgcggt cctttcaacg tatcggttca cgcacagcgc gctgcggttg
     3301 ccgccttgcg cgaccgcgaa catgtcgcgc gctccatcgc ccacaatgag acgtggaagc
     3361 gctggctcat cgaaagcatc cgtacgtcgg ggctccgtgt cgacgacagc gaaggaaact
     3421 tcgtgctcgt ccatttcccg cctggtgcat acagcgctgg cgccgccgac gcattcctca
     3481 ccgcgcgcgg gttcattctg cggccggtga cgaattacgg attgccgcat tgcctgcggc
     3541 tgaccatcgg cagcgaagat gccaatcgtg gcgtcgcggc ggcgatcgcg gatttcatgc
     3601 ggccggtgtg atgacgatcg attcgaaacc cctgttcggc aaggtggcgc tgatcggcat
     3661 cgggctgatc ggctcctcgc tcgcccatgc gatgcgccgt gcgggcctcg ccgcgcacat
     3721 cgccggatat tcccaccggt ccgagacggt cgaaaaagcc cgggcgatcg ggtttgcgga
     3781 ctcgttccac accgcgctcg cgccggcagt gcgtagcgcc gacctcgtcg tgctggcggc
     3841 gcccgtgggg gcgttcggag aactcgctcg cgaacttggc ccgcacctga aacggggcgc
     3901 aatcctgagc gatgtcggct ccgtgaagat ggcggtcctg cgcgatgtag ggccgcacgt
     3961 gcccgatggc gtgcatttca ttcccgctca tccgattgcg gggaccgagc agtcgggtcc
     4021 ggaatcgggc ttcgccgaat tgttcgacgg acgctggtgc atattgacgc cgctgcccgg
     4081 cgcggatgat ggggcggtcg ccacgctcaa atcattctgg cagcgctgcg gcagccaggt
     4141 ccagatcatg gatccgaagc atcacgatct ggtgctcgcc atcaccagcc acgtgcccca
     4201 tttgatcgcc tacaacatcg tgggcaccgc tgccgatctg gagaccgtga cggaaggcga
     4261 ggtgatcaaa tattcggccg gcggctttcg ggatttcacc cgcatcgccg cgtccgatcc
     4321 cgtcatgtgg cgcgatgtct ttcttaacaa tcgcgaggcg gtgctggaga tgctgggccg
     4381 cttcaacgag gatctgagcg cgcttcagcg cgcgatccgc tggggcgacg gcgagatgct
     4441 gttcaatctc ttcacgcgca cgcgcgccat ccgacgatcc atcatcgatg cgggccagga
     4501 catcggcgag cccaatttcg gccgtccgcg tagaaagaac ccaacgtagc gagactcctc
     4561 gcgacccctc acccacgcct ttacagcatg aatcgtttcc tcctgcatcc ctctccggca
     4621 aggggagagg ggaattcgga gtgctgcatc agcaacgtaa aatcagcgcg ccgattccct
     4681 ctcccgttgc ccgccttcgc caaggctacg gcgggtctga tgactctgct tttccgccga
     4741 agctttagcg tcggcagatg ggagaggggt cgagaacgag gaccgtccca gcgcgggtga
     4801 ggggtgccgc cgcgcaaaac gcgccgcact taccgcttga tggtcggtgt ggcgtcgtcg
     4861 tcgcgcagga tttcgtagtc ggcgaggccg gaacccatca gcatgcgcag tgcagcgagg
     4921 tccacggctt cgtcgtcgac gtgttgcgtc ttgtggatga gcgtcagctc accttcgctg
     4981 ttcagatagc ggatctcgta gttcggcacg gtgtccctcg gtgcaaaagt gcacccctcg
     5041 cttgcggacg ccacagatct aggaaggctt cgtgttgaga atgtggcgcc agacggttcg
     5101 aattgtcgca gagtgtgtca cgacattgtt acgagagtct ttctgaaggc attttagtaa
     5161 agcgcgtcga acgagcccgc actttccgcg taaggctggt gagcgtggcg tagcgtgacg
     5221 gctccattat tgaaggctgc ggaaacttcg agcggcacct cgccggtcga acgcgtggca
     5281 cgggccaggc ggccgagagc ccttgagagg cgcgcctccc gggcgggagg agtggaagtt
     5341 tggtccgttt gcgcaacttt tagttggaga gctccctggg ggcgatggag atcgtcgaga
     5401 gtcaaccggc ccgagccttc cgcgctggtt tggccccacg ccatgtcaag ccggtccagg
     5461 cgaaacgatc cggcactgcc gcgccatttg tccaatgcag cacaccaggg gccgcgtccc
     5521 gacaggaatg gagagaaagg cgccgcaggc gcgattcgtc ctacaaggac catgtgcggg
     5581 atttcgggac cgaattcggc ctgcaattcc ggcgcgagac gtagcgcctc ggcactgatc
     5641 gcgatatcga tcgtgtcgcg tgagggcgca cgccgaagat ggagttgtgc gcgcgcaccc
     5701 gaaacttccc gtgagccgaa accggctaaa tccagatcga agcgcgccag cacgccctcc
     5761 gagaggatcg cgcttgcccg cagcgaacca ggcgtgaaca ccaagcggcg cgcgccaccc
     5821 tccggcatcg tccacgacaa agtctgcgcg cctgcggctt cgaatatctt ttgcgcgcgc
     5881 ccgtagatca ggctgtggat tgcgaaacgt tcggcgcgcc aggagaccga tgcgcgcgcg
     5941 caattcgcct caagcgcaag gccgctcagt acggtgtcga cacgaaaggg aaagccgccc
     6001 atttcttcgg aggcaaagcg cagacggatg ccgggcgcga tttcacgtcc gttcgcgtga
     6061 agaagccagc gctcgaatgt accggcgaca acccaccagc gcagcatcgc cgcaatggcg
     6121 agcacgatga gcagcacaaa cggcagatag agaaagagac ggcttgaata gcgcatgctc
     6181 atgcttcgac aggctcagca tgaggaaatc tcctcaccct gagcttgtcg aagggtgagg
     6241 ccgccggccg agcgcagata tttacccgca gcgcgaaaag ccgcaggcga tgcagacgtc
     6301 gcagttctcc tgccggatga aggatgggct gccgcagcgt gggcaggtgc ggccgcggcg
     6361 ttcgcccgcg gccaccgcga ttgactccgc cgtttcgggc cgcgcgaggc gatcgcgcgg
     6421 cagcatgaag cccgtggcga cgaggtgccg ttcgatcaca tcgccgatcg ccgcgagaag
     6481 cgaaggcacg tactgcccgc ccatccattg tcccccgcgc ggatcgaaca ccgctttgag
     6541 ctcgtcgacc acgaaggaga cgtccccgcc gcggcggaac accgcgctga tcatgcgcgt
     6601 gagcgcgagc gtccatgcat aggcctccat gttcttggag ttgatgaaca cttcgaaagg
     6661 ccgccgccgc gtgccgccgc gcggatcgtc gaccaggata tcgttgaccg tgatgtagat
     6721 cgcgtgatcg ctttccggcc aatgcagctt gtaagtgcgg cccggcagcg tctcgggacg
     6781 cgcttcaggc gcactgtcgc cgtcatcgac cacgggcttt gcggctttct tcacctcgag
     6841 cacggcgccg cg
//
LOCUS       DASZMC010000253         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2580027,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000253 DASZMC010000000
VERSION     DASZMC010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580027"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..590
                     /locus_tag="VG274_05140"
     CDS             288..590
                     /locus_tag="VG274_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06072.1"
                     /translation="MKIATLALASLFALSSSFAFAQSGGATGGTSSSGMSTSGNAVTA
                     GPNTGMPNGMTTGSAAGNHNTDSSINPLGGPAAPHDSADNPALSPSPSAPASPTNK"
     gene            complement(824..994)
                     /locus_tag="VG274_05145"
     CDS             complement(824..994)
                     /locus_tag="VG274_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06073.1"
                     /translation="MPDTWSWEYQIGRHIKSGRLKAPSRKVALRSIYQKIDRDLREIH
                     LKAGSRYHQQSC"
     gene            984..1481
                     /locus_tag="VG274_05150"
     CDS             984..1481
                     /locus_tag="VG274_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06074.1"
                     /translation="MSGIAVCSKLNWIPLWIIRVAPRAAWRRLLDKPKRFGRIGGEPK
                     SAFGTRRDIVIAGRRVLVPVVFANRAVFSVNLIRRVLRKMLPLLPPYDSSALEFGISV
                     SGDSMKPIDDRPRAADERMFYDDARHTGGERDARQQAKERQDQSHAALESWIAAAIAE
                     LAVER"
BASE COUNT          299 a          465 c          473 g          305 t
ORIGIN      
        1 cggcgagcat ggcggtggcc tccatcaaat ccaacggtgg cggcgtcgcg ccacgctggc
       61 gagacaattc cggcaagcct gctcatagat cagattgatg atgagtgccc acatggccga
      121 accccgttgt tgaccgaccc gatgaaggcc tctctttgtt tcaggacgat ttcgtcggcc
      181 cggcaatctg gtttcgtcgg gggagtgaag cgcttaggga caaccggccg cgacaggaac
      241 gaaacgggat gtttccgatt ggttgaatct caactgagga aaccaccatg aaaatcgcaa
      301 cccttgcact agcctccctg ttcgcgctct caagctcgtt cgccttcgca caatccggtg
      361 gcgccactgg cggaacctcg tcgtcgggca tgtcgacatc cggaaatgct gtcacggctg
      421 gcccaaatac gggaatgccg aacggcatga cgaccggcag tgcggcgggc aatcacaaca
      481 ccgacagttc gatcaatccg ctcgggggcc ctgcggcgcc ccacgacagc gccgacaatc
      541 cggcgctttc gccgtcgcca tcggctcccg cttcgccgac gaacaagtga gataaaaaga
      601 aagccccgtg ctggagtgag cggggctttc ttcggcgtca agcatcctgg gatgccgcgt
      661 tgaaaacgcg gatcgccgaa aaaggttcat tgcgctgtta tcggcggatc gccttcagtt
      721 cgacccgccg aacgcggccg atgaacgggg ttcatcggac ctgaatctgg cgggacgttc
      781 cggcgcttgt tcggtgatcc cgcaagcgcg gaacgagccg cgttcagcac gattgctgat
      841 ggtacctgct gcccgccttc aaatggatct cccgtagatc gcggtcgatc ttttgataga
      901 tgctgcgcag cgccaccttg cgcgagggcg ccttcagccg cccgctcttg atgtgccgtc
      961 cgatttgata ttcccagctc caggtgtccg gcatcgccgt ttgctcaaag ctgaattgga
     1021 ttcccttgtg gatcattcgc gttgccccgc gtgcggcatg gcgccgcttg ctcgataagc
     1081 caaagcgatt cgggcgcatc ggtggcgagc ccaagtcagc attcggcacg cggagggata
     1141 ttgtaattgc gggacggcgc gtgctcgtgc cggttgtatt cgcaaatcgt gctgtgtttt
     1201 cagtaaactt aattcgtcgc gttttgcgca aaatgctgcc gctattgccg ccctacgata
     1261 gcagcgccct agaatttggt atcagcgttt cgggggacag catgaagccg atcgacgatc
     1321 gcccgcgcgc agcggacgaa cgcatgtttt acgatgatgc acgtcatacg ggaggcgagc
     1381 gcgacgctcg ccagcaggcg aaggagaggc aggatcagtc ccacgccgcg ctggagagct
     1441 ggatcgccgc cgcgatcgcc gaactcgctg tcgaacgcta gcggccttcg gccggcggcg
     1501 aattgggccc gagcagcctg aagtcgacat tgccgaaacc gg
//
LOCUS       DASZMC010000254         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_393140,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000254 DASZMC010000000
VERSION     DASZMC010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393140"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VG274_05155"
     CDS             complement(<1..439)
                     /locus_tag="VG274_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06075.1"
                     /translation="MTVVSALARAPALESEPVLPRSIDDAAITRLVCAPLMRSAGRTW
                     WIAFLVCSAGTLAPLYGIFTLFTRGVGIWGINTSVVWQLRDRKLCVVDWDWQRWHADL
                     LAAPAHAAVLARLDQPVCGGDDAFRGGDRGPVSDLPSRAPALFL"
     gene            complement(436..>1670)
                     /locus_tag="VG274_05160"
     CDS             complement(436..>1670)
                     /locus_tag="VG274_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWE06076.1"
                     /translation="DPSVFDGRFANNAWLQECPQPFTKAVWGNAIEMSPEDAAREEIA
                     EGDEAAIVADGRTVFGPVRLSNGLTPGVLRLSLGYGRQRAGAIGDGVGFNASILRNSA
                     SPWTTRGATLRRAGRDGKLPPSTAGLFALPGEVQKLAPLLRAGEAIPAPAPQPSFNSP
                     PPPLAGDPYAWAMVIDTGACIGCNACVVACQSENNVPSIGPDEIRMGRVMHWLRIDSY
                     EHGPAPNPEPVFQPIPCMHCEAAPCEPVCPVEASVHDHEGLNVQVYNRCVGTRFCESN
                     CPYKVRRFNVFGYADGQEFRNLGDPLVKAANNPDVSVRARGVMEKCTYCVQRISGARR
                     LAERESRLIEDGEVVTACQAACPTQAIRFGNLADPNSAVSRLRLDPRHYTLLEELHTR
                     PRTTYLARIADSESASKT"
BASE COUNT          303 a          528 c          569 g          270 t
ORIGIN      
        1 gtagaaatag agcgggcgcc cgagatggaa gatcggaaac aggcccgcga tcgccaccgc
       61 gaaaagcgtc atcgcctccg caaaccggct gatcgaggcg cgccaggact gccgcgtgag
      121 caggagcagc gaggagatca gcgtgccagc gttgccaatc ccaatccacc acacataatt
      181 tgcgatcgcg tagctgccag accacgctgg tgttgatgcc ccagatgccc acgccgcggg
      241 tgaagagcgt gaaaattccg tatagcggtg ccagcgttcc cgccgaacag acgagaaagg
      301 cgatccacca cgtccgtcct gccgagcgca tgagcggcgc gcagacgagg cgagtaatcg
      361 cggcgtcgtc gatcgagcgt ggaagaaccg gttcgctttc aagcgcaggc gcgcgggcga
      421 gcgcggagac gacggtcacg ttttcgacgc cgattcggag tcggcgattc gcgcgagata
      481 ggtcgttctc ggcctagtgt ggagttcctc cagcagcgtg taatgtcgcg gatcgagccg
      541 caggcgcgac acggcggaat tcgggtcggc gagattgccg aaccggatcg cttgcgttgg
      601 gcacgcggcc tgacaggcgg tgacgacctc gccgtcttcg atgagccggg actccctctc
      661 tgcgagccgc cgcgcgccgc tgatgcgctg gacgcaatag gtgcatttct ccatcacgcc
      721 gcgggcgcgc acgctgacgt ccggattgtt ggcggctttg accagcggat cgccgaggtt
      781 tcggaactcc tgtccgtccg cgtagccgaa gacgttgaag cggcgcacct tgtaggggca
      841 attggattcg caaaaccggg tgccgacgca gcgattgtag acctgtacgt tgaggccttc
      901 atggtcgtgc acggaagctt cgactgggca gacgggctcg cagggcgccg cctcgcaatg
      961 catgcacggg atcggctgga agacgggctc cggattcgga gccggcccat gctcgtaaga
     1021 atcgattctg agccaatgca tgacgcgacc catgcggatt tcatccggtc cgatcgaagg
     1081 gacgttgttt tccgactggc aggcgacgac gcaggcgttg cagccgatgc aggcacccgt
     1141 atcgatcacc atcgcccagg cataagggtc gcccgcgagc ggagggggcg gcgagttgaa
     1201 gctcggctgc ggtgcgggcg ccgggatggc ttctcctgcc cggagaagcg gcgcgagctt
     1261 ttgcacctct cctgggagcg cgaacaggcc ggcggtggaa ggcgggagct ttccgtcgcg
     1321 ccccgcgcgg cgcagcgtcg cgccgcgcgt ggtccagggc gaggcgctgt tccgcagaat
     1381 cgaagcatta aagccgacgc cgtcgccgat cgcgcccgcg cgctgtcggc catagccgag
     1441 cgacagccgt aacaccccgg gcgtgagccc gttcgaaaga cgtaccggcc cgaacacggt
     1501 ccggccgtcc gcaacgatcg ccgcctcgtc gccttccgcg atctcctcgc gggcggcatc
     1561 ctcgggcgac atctcgatcg cgttgcccca taccgccttc gtaaagggct gcggacattc
     1621 ctgaagccag gcgttgttcg cgaaccgccc gtcgaacaca gatggatcgg
//
LOCUS       DASZMC010000255         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5325949,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000255 DASZMC010000000
VERSION     DASZMC010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5325949"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VG274_05165"
     CDS             complement(<1..450)
                     /locus_tag="VG274_05165"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE06077.1"
                     /translation="MQKIRPCLWFDGKAEDAAKFYTSVFPNSKITDRMVHNEAGPGPK
                     GSVLTVTFELDGQEYVALNGGPHYTFTPAISFFVTCETQKEVDDYWDKLLAGGAPLQC
                     GWLTDKFGVSWQIVPRVLGEMLQDKDSARAARVMRAMFKMVKLDIAAL"
     gene            complement(680..1933)
                     /locus_tag="VG274_05170"
     CDS             complement(680..1933)
                     /locus_tag="VG274_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWE06078.1"
                     /translation="MTEIAWIHAALTAARPQAIGALLRYFRDLDTAEEAFQDACLRAL
                     KNWPQNGPPRDPTAWLILVGRNAALDGVRRRSRSSPLPPDELISDLDDAEATLVERLD
                     GSEYRDDVLRLLFICCHPDLPNTQQIALALRIVSGLSVKQIAHAFLVGESAMEQRITR
                     AKARIATAEVPFEAPGAVERAERLGAVAAMIYLVFNEGYSAGAGPARLQLCEEAIRLA
                     RLLLRLFQTEPEIMGLTALMLLQHARKDARFDARGEIILLEDQDRGRWDEKLIAEGQA
                     LIDKAMRHREPGPYQIQAAIAALHGRADRAQDTDWAQIDALYATLERVQPSPVVTLNR
                     AVAVSKLRGPAAALAMIEPLQPRLSGYFYFFGTKGALLLQLARHEEARTAFDHAIALA
                     NTPAEAAHIRQHLDRLQRESAPAAR"
     gene            complement(1930..2310)
                     /locus_tag="VG274_05175"
     CDS             complement(1930..2310)
                     /locus_tag="VG274_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWE06079.1"
                     /translation="MLYAILCYNDEDVVWSWSKEQDQAVMARIGVVNERLTASGKLGP
                     SLRLLPTTSATTLRKTQEPPLVIDGPFAETKEQLLGFYIIDVAGLDEALEIARELAQA
                     NPGGAYELRPVALYNPDRRAGAHG"
     gene            2585..2815
                     /locus_tag="VG274_05180"
     CDS             2585..2815
                     /locus_tag="VG274_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06080.1"
                     /translation="MRTTLAIDDDVLAAAKGLAARQRKSVGEVISALARTALRRTSYG
                     KEKRNGIPLLVKRRGAAPVTPELVRRLSDELP"
     gene            2812..3246
                     /locus_tag="VG274_05185"
     CDS             2812..3246
                     /locus_tag="VG274_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HWE06081.1"
                     /translation="MTVFLLDVNVLVALIDPSHIHHDGAHEWFAAEGQSAWATCPLVE
                     NGVLRVVGHPRYPNSPGSPAAVAQMLRIMCELPGHVFWPDDVSVLDAAALDTGRLLAS
                     SQVTDTYLLALARAHNGKLASFDRRLVVDAITGGARYLHAIP"
BASE COUNT          568 a         1057 c         1064 g          627 t
ORIGIN      
        1 cagcgcggcg atgtcgagct tgaccatctt gaacatggcc cgcatcaccc gggcggcgcg
       61 agccgaatcc ttgtcctgca gcatttctcc cagcactctg gggacgatct gccagctgac
      121 gccgaatttg tcggtgagcc atccgcattg cagcggcgcg ccgccggcca gcagtttgtc
      181 ccaatagtca tcgacctcct tctgcgtttc gcaggtcacg aagaaggaga ttgccggcgt
      241 gaaggtgtaa tgcggaccgc cattgagggc cacgtattcc tggccgtcga gctcgaaggt
      301 cactgtgaga actgaacctt tcggtccggg gccggcttca ttgtggacca tgcgatcggt
      361 gatcttcgaa ttcgggaaca ccgaagtgta gaacttcgct gcgtcctcgg ctttgccgtc
      421 gaaccacagg catggtcgaa tcttttgcat cggacaatct ccctgtttga gttccatact
      481 gatacccgct tttggacgta accaattcgt ggaacaacaa gattctaacg cagtggacgc
      541 agaggttgcg cagaggacgc agaggaaaac ctggcgcgcc aggtgttcgg gttaaacccg
      601 acctttccgc gcccatgttc ttcacgcgtc caccgcgcaa cctctgcgtc cactgcgctg
      661 aatgttttat gcgtatcact catctggctg ccggtgcgct ttcgcgctgc agacgatcca
      721 gatgctgacg gatatgggcg gcctcggcgg gggtgttggc cagcgcgatc gcatggtcga
      781 acgcggtgcg cgcttcctcg tggcgggcga gttgcaggag cagcgcgcct ttggtgccga
      841 agaagtaaaa atagcccgag aggcggggct gcagcggctc gatcatcgcc agcgcggctg
      901 cggggccgcg aagcttgctc accgcgaccg cacgattgag cgtcacgacc ggcgagggct
      961 gcacgcgttc gagcgtcgca tagagcgcgt cgatctgcgc ccagtcggtg tcctgcgcac
     1021 ggtcggcgcg cccatgcagc gcggcgatgg ccgcctggat ctggtagggc ccgggctcgc
     1081 gatgccgcat cgccttgtcg atcagcgcct gcccttcggc aatcagtttt tcgtcccagc
     1141 ggccgcggtc ctggtcctcg agaagaatga tttcgccccg cgcatcgaag cgggcatcct
     1201 tgcgcgcatg ctgcagcagc atcagcgcgg tcaatcccat gatttccggt tccgtctgga
     1261 acaatctgag cagcagccgc gccagccgga tcgcttcttc gcagagctgc aggcgcgccg
     1321 gaccggcgcc cgccgaatag ccttcgttga agacgagata gatcatcgcc gcgacggcgc
     1381 cgagccgctc cgcacgttcg acggcgcccg gcgcttcgaa cggcacttcg gccgtcgcaa
     1441 tgcgcgcctt ggcgcgggtg atgcgctgtt ccatcgcgct ctcgccgaca agaaaggcgt
     1501 gcgcgatctg cttcaccgac aatccggaga cgatgcgcag cgccagcgcg atctgctgcg
     1561 tgttcggcag atccggatga cagcagatga acagcaggcg caggacatcg tcgcgatatt
     1621 ccgagccgtc gaggcgttcg accagagtcg cctcggcatc gtcgagatcc gagatcagct
     1681 cgtccggcgg cagcgggctg cttcggctcc tgcggcgaac gccgtcgagc gccgcgttgc
     1741 ggccgaccag gatcagccag gcggtcggat cgcgcggcgg gccgttctgc ggccaattct
     1801 tgagcgcccg gaggcaggcg tcctggaatg cttcctcggc ggtgtcgaga tcgcggaaat
     1861 agcgcagcag cgccccgatc gcctggggcc gcgcagcggt cagcgcggcg tgaatccagg
     1921 cgatctcggt catccgtgcg cgcccgcgcg ccggtcggga ttgtagaggg ctaccgggcg
     1981 cagctcgtag gcgccgccgg gattggcctg cgccagttcg cgggcgattt cgagcgcctc
     2041 gtccagcccc gcgacatcga tgatgtaaaa gccgagcagc tgttccttgg tctcggcgaa
     2101 cggcccgtcg atgacgagcg gcggttcctg cgtcttgcgc agggtggtgg cggaggtggt
     2161 cggcaacaga cgcagcgacg gacccagttt gcccgaggcg gtcagccgct cgttcaccac
     2221 gccgatccgc gccatgacgg cctggtcctg ttccttgctc caggaccaga cgacgtcctc
     2281 gtcattgtag caaaggatgg catagagcat gcgtgaggct cccgcgtctg tcattaagac
     2341 gcgcgactat gcccgttccc gacagcccaa ggcaaaattc gtgaggccgc gggcttacgg
     2401 gttcctcccc gcgcgcagcg cgggggagga accagtcccc actcactctc gcgtcgttga
     2461 acactttccc gtcgtgcatg acgcattgaa ctttctgcag ctcacccaca tcacccgttg
     2521 acttcagcgg tggcgagacc ccatgatgtt cgatgcgtga tgattgatga atcgcgaaaa
     2581 aacgatgcgc accaccctgg ccattgacga cgatgttctc gctgccgcca aagggctggc
     2641 ggccagacag cggaaaagtg tgggtgaagt catttccgca ctggcgcgta cggcgctgcg
     2701 cagaacctcg tacggcaagg aaaagcggaa cggcattccc ttgctggtca agcggcgcgg
     2761 cgccgcgccc gtgacacccg aacttgtgcg gcgattgtcc gacgagttgc cgtgacggtt
     2821 tttctgctcg acgtaaacgt gctcgtcgca ctcatcgacc cctctcacat ccatcacgac
     2881 ggcgcgcacg aatggttcgc ggcggagggc cagtctgcct gggcgacatg tccgctcgtc
     2941 gagaacgggg tgctgcgtgt ggtcggacac ccgcgttatc cgaactcgcc tggttcgccc
     3001 gcggccgtcg cgcaaatgct gcgaatcatg tgcgagttgc cgggacatgt cttttggccg
     3061 gacgatgtga gcgtgctcga tgctgccgcg ctcgataccg ggcgtctcct ggcatcgagt
     3121 caggttacag atacgtatct gctcgcgctc gcccgcgcgc ataacggcaa attggccagc
     3181 ttcgaccgcc gcctggtggt cgacgccatt acgggcggag cgcgatactt gcacgcaatt
     3241 ccgtagcgga cgaatactca gaccgcgaga acgcctgcgt gcgcccactt ttcctccccc
     3301 gtcgtgggct atggga
//
LOCUS       DASZMC010000256         5468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4238489,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000256 DASZMC010000000
VERSION     DASZMC010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4238489"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VG274_05190"
     CDS             complement(<1..460)
                     /locus_tag="VG274_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWE06082.1"
                     /translation="MPSRAPLKVVRNWVCDSRQWDGYKAREGDVIIATAPKAGTTWTQ
                     HIVNLLVFQSSEPRPLFDLSPWIDCRFMMPIEAMLPMIEAQQHRRFMKSHLPFDALPI
                     HDEVRYIHVARAGLDACMSFLNHYNSFLPELLERLDAVGMADATIGRPFPR"
     gene            complement(835..2352)
                     /locus_tag="VG274_05195"
     CDS             complement(835..2352)
                     /locus_tag="VG274_05195"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HWE06083.1"
                     /translation="MLALVKETDLDWRVCSRARLSRDPRFDGKFFIAVRGSRVYCRSI
                     CPAPTANEENCRYFATAAAAAEAGYRPCLRCRPESSPGTPAWAGTSATVSRALRLIDE
                     MGSEDGGMETLAERLGVGSRHLRRLFLRHLGATPKSVVQTRRMDFAKKLIDETNLPMT
                     ELAIAAGFGSVRRFNAGIRKTYHRTPTQIRTIARQRVALPENEYEFRLRFRPPYSWKG
                     MLAFLAESATPGVEDIGSGAYRRTIALNGTDGSFEVSLDESRLALRARIRFGDPRALY
                     FIVERIRALFDLNADWAKIARSLVADPMLSKRLKADPGLRVPGCWNGFELAVRAIIGR
                     DVGSQDARDRAARMARAFGRPFPSDNGVTHLFPEPGTLAGADLAGIGLPKARADAIRA
                     LARAVCDGDIAFEGVVDSDAFLRRLRKVPGIDAWTAQYVAMRALDEPDAFPFEREDLM
                     RALGLNDPAALDLRTERWRPWRAYAAMYLWAAADLDLARTEAKKTPHAPLRRRSA"
     gene            2585..3796
                     /locus_tag="VG274_05200"
     CDS             2585..3796
                     /locus_tag="VG274_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06084.1"
                     /translation="MKRAILTCTTAMVSLGMAAMPAHAAPGNTATLKPTAVPTAQVVY
                     DVARLGNPLGGSYGQGSSITNRGEVAGFDALPGNTVMHAVRWRNGYAPQDLGTLGGPN
                     SAIAWPNRNESGAMAGVAETSTPQPRGETWSCAAAVFYLAPPTGDVCLGFKDQNGAMS
                     ALPTLGGDNGFATGINGSGQIVGWAENTVQDPTCNAPQVLQFEAVVWGPKGQVTQLPA
                     IENDPDSAATAINDQGQVVGISGICSNAIGGLSAAHMVLWQNGAATNLGSLGGIAWNT
                     PMDISQKGHVTGFSDLPGDGGNSPNFHAFFWAPKGGIQNLNTLPGDAISEGLGINDKD
                     QVVGASYPSSHAFIWQNGVMTDLNNLVAPGTTLVLIDAQEINDAGAITGEAQDPNTGA
                     ISAFVATPKPK"
     gene            4261..>5468
                     /locus_tag="VG274_05205"
     CDS             4261..>5468
                     /locus_tag="VG274_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06085.1"
                     /translation="MNLRNPFLQLVAATAIAGAAWGAAELAPNSPDPWLWLTDIHGAR
                     PLAWAKAQNAKSFAALQADPEYRRDHDAILKLLNAEDRIPMPEIENGVVFNFWQDAAH
                     VRGLWRRTTAAGYAQAQPHWDVLLDVDGLDATERKPWVWQGAQCAPNMKRCLVRLSPG
                     GGDASAVREYDVVKKAFVIGGFSLPVAKSDTAWVDDDTILVGSDFGPGSMTTSGYPRF
                     VRMWHRGQALSAAKTIFAGKTSDVASRPTTFHGPYGIVPLVERGLTFFTSEFYFVTPD
                     GRTVQLPLPPGANVKGVTGGNLIFTLRDDWTPKGGRPIAKGSLVAFPVVAFVKGNASP
                     AYTVLATPDARGAIEDVAPGRDAIYASIFENVTGKIHEFRPGAGGAWTHTILDLPLGG
                     STHIADADAWT"
BASE COUNT         1041 a         1762 c         1729 g          936 t
ORIGIN      
        1 cacgggggaa aggccgccca atggttgcgt ccgccatgcc aacggcgtcc aggcgctcca
       61 gcaactcggg aagaaagctg ttgtagtgat tgaggaacga catgcacgca tcgaggccgg
      121 cgcgcgccac gtggatgtag cggacttcgt catggatcgg cagcgcgtcg aatgggaggt
      181 gggatttcat gaagcgccga tgctgctgcg cctcgatcat gggaagcatc gcctcgatcg
      241 gcatcatgaa ccggcaatcg atccatggcg acaagtcgaa cagcggacgg ggttcggacg
      301 actggaagac cagcaggttg acgatatgct gggtccaggt ggtgccggcc tttggcgccg
      361 tggcgatgat gacatcgccc tcgcgtgcct tgtagccgtc ccactgccgg ctgtcgcaga
      421 cccagttcct gacgaccttc aaaggcgcgc ggctcggcat ggctacgctc ctattgccgc
      481 ccggcgattt gcgccttcgg cgttagcacg gtggcgaaga acgccacgaa ggtcagcgaa
      541 ttgaagatca tcccggcgcc cctggccgag aacggactcg gtccccgttc acatcctgtc
      601 aggagaggac taatggttca atgggcgatg cgctggcgcc gcgcgcgctc actcgttcaa
      661 aagtaatact gtcatggtcc gcgcaagcgg gccacccagc tgaaacctgc aaagtcgtgg
      721 gtttgtaagc gcgagttctt gttgtcctgt ggtccggtcg tcgctccaat ctgggtggcc
      781 cgcttgcgcg ggccatgaca aggtaagttt acgtcttttg acttcgataa tgccctacgc
      841 gctgcggcgg cgaaggggtg catgcggcgt ctttttcgcc tcggtgcgtg caaggtccaa
      901 gtcggcggcg gcccagagat acatggccgc ataggcccgc catggccgcc acctctcggt
      961 gcgcagatcc aatgcggcgg gatcgttcaa cccgagcgca cgcatgaggt cttcgcgctc
     1021 gaacggaaac gcatcgggct catccagtgc gcgcatcgcg acatattgcg ccgtccaggc
     1081 atcgatgccg ggaactttgc gcaaacgtct gaggaacgca tcggaatcga cgacgccctc
     1141 gaatgcgatg tctccgtcgc acaccgcccg cgccagcgca cgaatcgcat cggcgcgcgc
     1201 tttcggcaac ccgatcccgg ccagatcggc gcccgcgaga gtcccaggct ccggaaagag
     1261 atgcgtcacg ccgttgtccg atggaaacgg ccgcccgaac gctcgcgcca tgcgggctgc
     1321 gcgatcgcgc gcatcctggc tgccgacatc gcgcccgatg atcgcgcgca cggcaagctc
     1381 gaacccgttc cagcaaccgg gaacacgcaa gcccggatcc gctttcagcc gtttggacaa
     1441 catcggatcg gcgacaagac tcctggcgat tttcgcccag tccgcattga gatcaaacag
     1501 cgcgcgaatg cgctcgacga tgaaataaag cgcccgcgga tcgccgaacc ggatgcgcgc
     1561 gcggagcgca agtctgcttt catcgagcga cacctcgaac gaaccgtccg tgccgttcaa
     1621 cgcaatcgtg cggcggtagg cgccgctgcc gatatcctcg acgcccggcg tcgcgctctc
     1681 cgcaaggaat gccagcatgc ctttccagct gtaaggcgga cggaagcgca gccggaattc
     1741 atactcattc tcgggaagcg ccacgcgctg acgcgcgatg gtgcggatct gcgtgggcgt
     1801 gcggtggtag gttttgcgga tgccggcatt gaagcgccgc acggacccaa atcccgccgc
     1861 aatcgccagt tcggtcattg gcagattcgt ctcgtcgatc agctttttcg cgaaatccat
     1921 ccgccgcgtc tgcacgaccg atttgggcgt agcgcccaga tgccggagga acaggcgccg
     1981 caggtggcgc gagccaacgc ccaggcgctc ggcgagcgtt tccattcccc catcttccga
     2041 acccatttcg tcgatcaggc gcaatgcgcg cgacaccgtc gccgaagtcc ccgcccatgc
     2101 gggcgttccg ggcgaagatt ccggccggca gcgaaggcat ggacgatagc cggcttccgc
     2161 tgccgcggcg gcagtcgcga agtaacggca attctcctca ttcgcggtcg gcgccggaca
     2221 gatggaccgg caatagacgc ggctgccgcg cacggcaatg aagaactttc cgtcgaagcg
     2281 cggatcgcgc gacagccgcg cccgcgaaca gacgcgccag tcgagatccg tttccttcac
     2341 gagcgccagc acgttcgtgt cgcctcaccc aactcgtcgg ttccgcgaaa gcccaagaat
     2401 gacacggttt gtcgagcgca ttcgcgtgag aaccgagcat acgcgatttc gaggcgtcac
     2461 atggacgttt tcggacctcg ctgtgtccac atcgatgtcc gcaaacgtcc aaccctcttg
     2521 tgcaattgga cctatcgtct tggcgatgcg aggagcagcg ccaaaagcca aagaggagaa
     2581 cacgatgaaa cgcgcaatat tgacctgcac gacggcgatg gtgtcgctgg gcatggccgc
     2641 gatgccggcc catgcagcac ctggaaacac ggcgacgctg aaacccacag cagtgccgac
     2701 agcccaggtc gtgtacgacg tcgccagact gggcaacccg cttggtggaa gttacggcca
     2761 gggttcgagc atcaccaatc gcggcgaggt ggcgggattc gatgcgctgc cgggaaacac
     2821 ggtcatgcac gccgtgcgct ggcgcaacgg ttacgcgccg caggatctcg gtacgctcgg
     2881 cggacccaac agcgcaatcg cgtggccgaa ccgcaacgaa tccggcgcga tggcgggcgt
     2941 cgccgagacg tccacgccgc agccgcgcgg cgaaacctgg agctgcgcgg ctgcagtttt
     3001 ctacctcgcg ccgcctaccg gtgatgtctg cctcggcttc aaggaccaga acggcgccat
     3061 gtctgcgctt cccacgttgg gcggcgacaa cggatttgcg accggcatca atggcagcgg
     3121 acagatcgtc ggctgggcgg agaatacggt tcaggatccg acctgcaacg cgccgcaggt
     3181 gctgcagttc gaagcggtgg tgtggggccc gaaaggccag gtgacgcagc ttccggcgat
     3241 cgagaacgat cccgacagcg cagcaaccgc gatcaatgac cagggccagg tggtcggcat
     3301 ttcgggcatt tgctcgaacg ccatcggcgg cttgagcgcg gcgcacatgg tgctgtggca
     3361 gaacggcgcc gcgacgaatc tcggcagtct cggcggcatc gcgtggaaca cgccgatgga
     3421 catttcccag aagggccacg tcaccggatt ttccgacctg cccggcgatg gcggcaactc
     3481 gccgaacttt cacgctttct tctgggctcc caaaggcggc atccagaacc tgaatacgct
     3541 tcccggcgat gcaatcagcg aaggtctcgg catcaacgac aaggaccagg tcgtaggcgc
     3601 gtcctatccg tccagccacg cattcatctg gcagaacggc gtgatgacgg acctgaacaa
     3661 cctcgttgcg ccgggcacga cgctggtgct gatcgacgcc caggagatca acgacgcggg
     3721 cgcgatcacc ggcgaagcgc aggaccccaa caccggcgca atctccgcct ttgtagcaac
     3781 gcccaagcca aaataaccgc agccttgttt cctcccccaa cgttgggggg gcgcgtatct
     3841 gaatctcaaa agaaacttga tccgcgcatt ctttttcctc ccccacgctt tggcgtgggg
     3901 gaggtgcctc ccaccggtcc gcgcgaagcg cggccggagg acaacctccg gggcggaggg
     3961 ggcaagcggc ggccacgcca cttaccccct ccgacgcttc ccccggctca aggccggggt
     4021 cgcgccacct cccccgcgaa agtgcgggcg aggaaaccag actagcgacg gcggtggttg
     4081 agcaacgcgg tgatgttcct cgatatctga atcaacgcag cgatgttggc gttcgcaacg
     4141 cagaatctcc ggtgatcgct cgcgccaatg tgatcgggcc ggattattcg gcagcgctat
     4201 cccacgccgc cctgccgtgg aatattccgc tcgaacagcc gagcggggac gaaaccggtt
     4261 atgaatctcc gaaatccttt cctgcaactc gtggcggcga ccgccatcgc cggcgctgcc
     4321 tggggcgccg cggaacttgc gcccaattcc ccggatccgt ggctctggct gaccgacatt
     4381 cacggcgcca gaccgcttgc ctgggcgaag gcgcagaacg cgaagtcctt tgcggccctt
     4441 caggccgatc cggaataccg cagggaccat gacgcgatcc tgaaactgct caatgcggaa
     4501 gaccgcatcc cgatgccgga gatcgagaac ggcgtcgtct tcaatttctg gcaggatgcc
     4561 gcgcatgtgc gcggactgtg gcggcgcacg accgctgccg gttacgcgca ggcgcaaccc
     4621 cactgggacg tgctgcttga cgtcgacggg ctcgacgcca cggaacgcaa accctgggtc
     4681 tggcagggcg cgcaatgcgc gccgaacatg aagcgctgtc tcgttcgtct gtctcccggc
     4741 ggcggcgatg cctcggcggt tcgcgaatat gacgtcgtca aaaaggcgtt tgtcatcggc
     4801 ggattctcac tgccggttgc caaatcggac accgcatggg tcgacgacga cacgatcctg
     4861 gtcggatcgg attttggtcc gggttcgatg acgacgtccg gctatcccag gttcgtaagg
     4921 atgtggcatc gcggccaagc gctatcggcc gcaaaaacga tcttcgccgg caaaacgagc
     4981 gatgtcgcgt cgcggcccac gacctttcac gggccatatg gcatcgtgcc gctggtggag
     5041 cgcggcctca ctttcttcac gtcggaattc tattttgtga cgccggacgg caggacagtg
     5101 caactgccgc tgccgcccgg cgcgaatgtg aaaggcgtca ccggcggcaa tttgatcttc
     5161 accctccgcg acgactggac gcccaaaggc ggcaggccaa tcgcgaaagg ctcgctcgtg
     5221 gcattccccg ttgtggcttt cgtgaaaggg aacgcgtcgc ctgcctacac cgtgctcgcc
     5281 acgcccgatg cgcgtggcgc gatcgaggat gtcgcgccgg ggcgagacgc gatctacgcg
     5341 tcgatcttcg agaatgtcac tggcaagatc cacgaatttc gtccgggcgc gggtggtgca
     5401 tggacccaca cgattctcga tctgccgctg ggcggctcga cgcacatcgc ggacgcggat
     5461 gcctggac
//
LOCUS       DASZMC010000257         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4778934,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000257 DASZMC010000000
VERSION     DASZMC010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4778934"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VG274_05210"
     CDS             complement(<1..697)
                     /locus_tag="VG274_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06086.1"
                     /translation="MSVPASASPDTVRSFWHGAPLNPYLLLCLHSFARTGCRVEIFTY
                     GRALRFPDWIAQREAADILPAKEVLVYRNGLGIGSPALHSNLFRFAMLHQVGGWWIDT
                     DVALLRGPLPAAPCFFSVEENHFGNCVLKFPRGHQLTAEGAERCRALGADARWGAAGP
                     MLFTALVHGHGLAELAAPAESSRPFIWSDVAAMFDPHRCAEMMQRSSAATFMHLCWEA
                     WRRAGVPADLGPPV"
     gene            952..>1640
                     /locus_tag="VG274_05215"
     CDS             952..>1640
                     /locus_tag="VG274_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06087.1"
                     /translation="MTAADRAQLDRELAQTRGFPVLFKLSDPEAPNYRDENTRYIFQQ
                     ADAPGVHYLSIYDPEGPLQAFVASLRRSKVLRLPFPYDATREVERGFGERRRRVFLSG
                     TQSRIGYPLRTALHRQRIFNPMLRFAVSELPHPGYADGGERLRHTLIFKAFVAHAAQF
                     SHFFLCGSRYRVELMKFVECAYAGSVPIGEPPKSLEQAVGDCFIRYSGRTGELLQALM
                     GDRSEMEVRAAA"
BASE COUNT          298 a          529 c          515 g          298 t
ORIGIN      
        1 ccacgggcgg gccaaggtcg gccgggacgc cggcgcgccg ccaggcctcc cagcaaagat
       61 gcatgaaggt agccgcgctc gatcgctgca tcatttccgc acagcgatgg ggatcgaaca
      121 tcgcggctac gtcggaccaa atgaacgggc gggagctttc cgccggcgcg gcaagctccg
      181 cgagtccgtg cccatggacc agtgcggtga acaacattgg gccggcagcg ccccagcgcg
      241 catcggctcc aagcgcgcgg cacctctccg cgccttcggc tgtcaactgg tggccgcgcg
      301 gaaatttcag cacgcagttg ccgaaatgat tctcctcgac ggagaagaag cacggcgcgg
      361 ccggcagtgg cccacgcagc agggccacat ccgtgtcgat ccaccaaccg cccacctgat
      421 ggagcattgc aaaccggaac aagttggaat gaagcgccgg gctaccgatt ccgagtccgt
      481 tgcggtagac gagaacctcc ttggcgggca ggatgtcggc ggcttcgcgc tgcgcaatcc
      541 agtccggaaa gcgaagcgca cgaccgtagg tgaagatttc cacccgacag cctgtacgcg
      601 cgaacgagtg caggcacagc aataggtaag gattgagcgg cgcgccgtgc caaaagctgc
      661 gcaccgtatc aggactcgcg cttgccggaa cgctcatgcg cccgtcctgc agggcgattt
      721 cgcgatgatt gccaatctct gacgcaggcg ctccgattcg tgataaatat gcttgagtgg
      781 gcagagaaca gcgcggtgcg aatcgtctac tttctttcgc cgacgttcga tttcacgacg
      841 ttgatctatc cgggggtcgg gcccctctat gcgcagagct ttggctggcc gaccgcacca
      901 atcgatgcgc tgtcgcgcac accctgtgac gtcgccatca tcgacaaccg cttgaccgcg
      961 gccgaccggg cgcagctcga tcgcgagctc gcgcagactc gcgggtttcc ggtgctgttc
     1021 aagctgtctg atcccgaagc cccgaactat cgcgacgaga acacccgcta catcttccag
     1081 caggccgacg cgcccggtgt tcattacctt tcgatctacg atccggaagg cccgctgcag
     1141 gcattcgttg cgtcgctgcg tcggtcgaag gtattgcgcc tgccgtttcc ctatgacgcc
     1201 acacgcgagg tcgagcgcgg gtttggcgag cgccggcgcc gggtttttct ctctggcacc
     1261 cagagccgca taggctatcc cttgcgtact gcgctgcatc gccagcggat ctttaatccg
     1321 atgctgcgat tcgcggtcag tgagctcccg caccccggct acgcggatgg aggcgagcgc
     1381 ctgcgtcaca cgctgatatt caaggccttc gtcgcccatg cggcgcagtt ctcgcacttc
     1441 ttcctgtgcg gctcgcgcta tcgcgtcgaa ctcatgaagt tcgtcgagtg cgcgtacgcg
     1501 ggcagcgtgc cgattggcga gcccccgaaa tcgcttgagc aagcagtcgg cgactgcttt
     1561 atccgctatt cggggcgaac gggcgagttg ttgcaagcgc tcatggggga tcggagcgaa
     1621 atggaagtgc gcgccgccgc
//
LOCUS       DASZMC010000258         8728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3957393,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000258 DASZMC010000000
VERSION     DASZMC010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3957393"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /gene="glyA"
                     /locus_tag="VG274_05220"
     CDS             <1..892
                     /gene="glyA"
                     /locus_tag="VG274_05220"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWE06088.1"
                     /translation="SPVNQSGKWFRPVAYGVRQQDQLIDMDAIAALASEHRPKLILAG
                     GSAYSRVIDFARFREIADSVGAFFMVDMAHFAGLVAGGVHPNPLDHAHVVTTTTHKTL
                     RGPRGGMILAQDAQLGKKINSAVFPGLQGGPLMHVIAAKAVALGEALKPDFRLYAHAV
                     VENAKALAQTLQTLGLDIVSGGTDTHLMLVDLRPKGATGREVEKALDRASITCNKNAI
                     PFDSEKPAITSGIRLGTPAGTTRGFGTAEFREIGQWIVDIVDAVAQGESDAGAEGRVR
                     SRVEDLCRRFPIYMHSRPTQ"
     gene            909..1370
                     /gene="nrdR"
                     /locus_tag="VG274_05225"
     CDS             909..1370
                     /gene="nrdR"
                     /locus_tag="VG274_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWE06089.1"
                     /translation="MRCPFCGHDDSQVKDSRPSEDNSAIRRRRHCPACGGRFTTFERV
                     QLRELIVVKKNGRREPFERDKLERSLTHALRKRPVESERVDRMVTGIVRRLESMGENE
                     IPSSVIGELVMQALASLDKVAYVRFASVYRNFRETKDFEALIGEMEGGDRE"
     gene            1384..2472
                     /gene="ribD"
                     /locus_tag="VG274_05230"
     CDS             1384..2472
                     /gene="ribD"
                     /locus_tag="VG274_05230"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWE06090.1"
                     /translation="MNDIRHMRHALVLAARGLGRVAPNPAVGCVIVSPNGVIVGRGWT
                     GRGGRPHAETIALAQADEAARGATAYITLEPCAHRGETPPCANALVEAGVARVVAPIE
                     DPDPRVSGRGFEKLRAAGIDVAMGILAQEAAELNAGFFLRLRERRPFVTLKIAASFDG
                     RTTDSGGDSRWITGEESRRFGHLLRARHDAILVGAETAIADDPLLTCRIAGLEDRSPI
                     RVVLDTRLRLDESANLAKTACEVPTWVFGASEGGGALRARGVSVISVAKDARGRPDIG
                     AVLNELAARGVTRLLVEGGAAVLATFLDRRLADRVELFRAPIALGAAGHASVEALAAL
                     GLDEAPRFSSVGTQRLGPDLLESFTVRA"
     gene            2481..3080
                     /locus_tag="VG274_05235"
     CDS             2481..3080
                     /locus_tag="VG274_05235"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWE06091.1"
                     /translation="MFTGIVSDVGQVRHVEKRGDTHVVIATRFDVSALGIGASIACAG
                     VCMTVVDRGGATDCWFAVTASGETLSKTTVADWQVGHSVNLERPLRVGDELGGHIVTG
                     HVDGTAEIVAAAPEGESMRLTFEAPQALARFIAPKGSIALDGVSLTVNDVDGVRFGVN
                     LIPHTLAATTFSGARKGSRVNLEIDVLARYVARLAKGLA"
     gene            3080..4207
                     /gene="ribB"
                     /locus_tag="VG274_05240"
     CDS             3080..4207
                     /gene="ribB"
                     /locus_tag="VG274_05240"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWE06092.1"
                     /translation="MFRDYLSSMEELIEDARNGRMFILVDAEDRENEGDLVIPAQMCT
                     PQAVNFMAKHGRGLICLALTQERANALRIPMMTQFNGTPQKTAFTVSIEAKEGISTGI
                     SAHDRAHTISVAIDATKGPDDITMPGHVFPLVARHGGSLVRAGHTEAAVDIARLAGLY
                     PAGVICEIMNDDGTMARLPDLVQFAQLHGLKIGTIAELIAYRRRYDHFVQRTIESDFH
                     SHFGGLWKLMVYVNTIEYAEHIALVKGDLSGPKPVLVRMHALNIFTDMLGYEPYEKEI
                     LAESMRIVAGQGRGVVVVLRQTRPTFVSDLLSRRISDDIDRRRVKEYGVGAQILLDLG
                     ISNMILLTDTAEKKIVALEGYGLNIVGTHAIRGNRQPGGKS"
     gene            4204..4653
                     /gene="ribH"
                     /locus_tag="VG274_05245"
     CDS             4204..4653
                     /gene="ribH"
                     /locus_tag="VG274_05245"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWE06093.1"
                     /translation="MTPNILIVEARFYSNISDALLDGATAALDRAGAWSERIGVPGAL
                     EIPPAILFAAGVGEGGGKNYDGYIALGCVIRGDTYHFEIVANQSARGLMELGLRHGLC
                     IGNGILTCDTEEQAIARASRGELDKGGDAARACLALIETRMRLGATR"
     gene            4650..5168
                     /gene="nusB"
                     /locus_tag="VG274_05250"
     CDS             4650..5168
                     /gene="nusB"
                     /locus_tag="VG274_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWE06094.1"
                     /translation="MISPAPRNEIASGARAERRSARLAAVQALYQMELGGGDAETVAQ
                     EFLDHRFQREPELAEAGAPDEALFRALLAGVPHHQAEIDRAIASCLTASWRLERIDSI
                     LRAILRAAACELIVRHDVPARVVIDEYLEIAHGFCGEEERTFVNAVLDRLARRKRPAE
                     FGEAPPDEEPGV"
     gene            5149..6159
                     /gene="thiL"
                     /locus_tag="VG274_05255"
     CDS             5149..6159
                     /gene="thiL"
                     /locus_tag="VG274_05255"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWE06095.1"
                     /translation="MKNPEFEAKVRPGEFELIAKLFAPLSRGVSGAFALTDDVAVIEP
                     KPGCEFVLKTDSLIESVHFLRSDPAGTVAQKALRRALSDLAAKGAGPSVYLLALALPS
                     WPELNWLEAFAAGLAEDQAKFECALAGGETNATPGPVTVTVTAVGYVPQGALIRRNGA
                     TPGDSVFVSGTIGDAGAGLDLLANGDIPASAAARDFLVSRYRLPIPRLELGRELRGLA
                     SAALDVSDGLLADLGHIAEASQARIEIDAARVPMSDALRELRGEGISVRVGAAIAGDD
                     YEIAFTAPRSAADTILDVARRTGTQVTEIGRVVSGGGVALLDGSGNPIQMPRLGYTHF
                     RA"
     gene            6334..8460
                     /locus_tag="VG274_05260"
     CDS             6334..8460
                     /locus_tag="VG274_05260"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001951.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWE06096.1"
                     /translation="MGLQLELILASGVLALLYGAWTSRSVMAMSPGNARMQEIAAAIQ
                     EGAAAYLNRQYTTIAIAGVVILIIAFAIFGWQVALGYLIGASFSGVAGYVGMNVSVRA
                     NVRTAEASRHGLAAGLSIAFRSGAVTGLLVAGLGLIGVAGYYTFLRYGLHLDINDEAQ
                     GRLISNALVALGFGASLISIFARLGGGIFTKGADVGGDMVGKVEAGIPEDDPRNPATI
                     ADNVGDNVGDCAGMAADLFETYAVTTVATMVLASIYFTGGLRSALMLLPLGIAAMCIV
                     TSIIGTFFVQLGKNGSIMGALYKGVLATGALSLIGLWPLTDWLVGLDTVLGHDVTGRS
                     LFFCGAVGLAVTGAIIWITEYYTGSGFRPVRVIAEASRTGHGTNVIQGLAISMESTAL
                     PALVICVGIVVANWLAGLFGIAIAVSTMLSLAGMIVALDAFGPVTDNAGGIAEMAGLE
                     GDVRKTTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYTQDLKYFIAHSDVYGYFR
                     GLALPTFSLYDPWVVVGLFIGGLLPYLFGGIALTAVGRAAGAVVEEVRRQFREMPGIM
                     QGTQKPDYGRAVDMLTKAAIREMVIPSLLPVLSPIVLYVIVDNIAGKAQAFAALGAML
                     LGVIVTGLFVAISMTSGGGAWDNAKKFIEDGHFGGKGSDAHKAAVTGDTVGDPYKDTA
                     GPAVNPMIKITNIVALLLLATLAHGI"
BASE COUNT         1500 a         2754 c         2880 g         1594 t
ORIGIN      
        1 ctcgccggtg aaccagtccg gcaaatggtt tcggccggtg gcctatggcg tgcgacagca
       61 ggatcagctc atcgacatgg acgccatcgc cgctctcgcc agcgagcatc gtcccaagct
      121 catcctcgcc ggcggctcgg cctattcgcg cgtcatcgat ttcgcgcgct ttcgcgagat
      181 cgcagattcc gtcggcgcct tcttcatggt cgacatggcg cattttgcgg gccttgtcgc
      241 cggcggcgtg catcccaatc cgctcgatca cgcccatgtg gtgacgacca cgacgcacaa
      301 gacgctgcgc gggccgcgag gcggaatgat ccttgcgcag gacgcgcaac ttggaaagaa
      361 aatcaactcc gccgtgtttc ccggtcttca gggcggacct ctcatgcatg tgatcgcggc
      421 caaggctgta gcgctcggcg aagcgctgaa gccggacttc agactttatg cgcacgcagt
      481 cgtggaaaat gccaaggcgc tggcgcaaac gctgcaaacg ctcggactgg atatcgtatc
      541 gggaggcacg gacacccatc tcatgctggt cgatctgcgt ccgaaggggg cgacagggcg
      601 cgaggtggag aaggcgctcg atcgcgcatc cataacctgc aacaagaatg cgatcccctt
      661 cgatagcgaa aaaccggcga tcacgtcggg cattcggctc ggcaccccgg ctggaaccac
      721 gcgcgggttc ggaacggcgg agttcaggga aatcggccag tggatcgtcg acatcgtcga
      781 tgccgtcgcg cagggcgaaa gtgatgcggg cgccgaaggc cgtgtgcgtt cacgcgtcga
      841 agatctgtgc aggagattcc cgatctacat gcattcccga cccacccaat aaaggagagc
      901 acgcgcccat gcgctgcccg ttctgcggac atgacgattc tcaggtcaag gacagccggc
      961 caagcgaaga caattccgcc atccgccgcc gccggcattg tccggcctgc ggcggacgct
     1021 tcacgacttt cgaacgtgtc cagttgcgcg aactgatcgt cgtgaagaag aacggccgcc
     1081 gcgagccctt cgaacgcgac aagctcgaac ggtctctgac ccacgcactg cgcaagcggc
     1141 cggtggaatc cgagcgtgtc gaccgcatgg tcaccggaat tgttcgccgc ctcgaaagta
     1201 tgggcgaaaa cgagatcccg tcgagcgtca tcggcgagct ggtgatgcag gcgctcgcga
     1261 gcctcgacaa ggtggcttat gtgcgctttg catccgtcta tcgaaatttc cgcgaaacca
     1321 aggatttcga ggcgctgatc ggcgaaatgg agggcggcga ccgggagtga gctcttggag
     1381 cccttgaacg acattcgtca tatgcggcat gcgctcgtcc tggcggcgcg cggtttgggg
     1441 cgtgttgcgc ccaatcccgc tgttggctgc gtgatcgttt cgccaaacgg cgtgattgtg
     1501 gggcgcggct ggaccggaag aggcggccgg cctcatgccg aaacgatcgc tctcgcccag
     1561 gcggacgaag ccgcgcgcgg cgcgacggcg tacataacgc tcgagccctg cgcgcatcgt
     1621 ggcgaaacac cgccttgtgc gaatgccctc gttgaagccg gcgtcgcgcg cgtggtcgcg
     1681 ccgatcgagg accccgatcc gcgcgtcagt gggcgcgggt tcgagaaatt gcgcgcggcc
     1741 ggaatcgatg tcgcgatggg catcctggcg caagaagccg ccgaattgaa tgcgggattc
     1801 ttcttgcgcc tgcgcgagag gcggccattc gtcacgctca agatcgcggc cagtttcgac
     1861 ggacgcacca ccgattcggg aggcgacagc cgatggatca cgggcgagga atcccgccgc
     1921 ttcggccatc tgctgcgggc cagacacgat gcaattctcg ttggggcgga gacggcgatc
     1981 gctgacgatc cgctcctgac ttgccggatc gccgggctgg aggatcgttc ccccattcgt
     2041 gtggtgctcg acacgcggct gcggcttgac gagtctgcaa atctcgcaaa aacggcgtgc
     2101 gaagttccga cctgggtatt tggcgcgtcg gaaggcggcg gcgcgctgag ggcgcgcggg
     2161 gtgagtgtta tttcggttgc gaaggatgcg cgcggccgtc cggatatcgg cgcggttctg
     2221 aacgaacttg cggcgcgcgg cgtgacgcgc cttctggtcg aaggcggcgc ggccgtgctc
     2281 gcaactttcc tcgatcggcg cctcgccgac cgcgtcgagc tgttccgcgc gcccattgcg
     2341 ctcggggccg ccggtcatgc ctccgtcgag gcgctcgccg cattgggact cgacgaggcg
     2401 ccgcgctttt cgagcgtcgg aacgcagcgg ctcggaccgg acctgctgga aagcttcacg
     2461 gtcagggcct aaaagaaccc atgttcacgg gaattgtcag cgatgtcggc caggtgcgcc
     2521 atgtcgagaa gcgcggtgac acgcatgtcg taatcgccac gcgtttcgat gtgtccgcgc
     2581 tcggtatcgg cgcctcgatc gcctgtgccg gcgtctgcat gaccgtggtc gaccggggcg
     2641 gcgcgacgga ctgctggttt gcggtcacgg cttcgggcga aactctgtcg aaaacgaccg
     2701 ttgcggactg gcaggttggc cactccgtca atctcgaacg gccgctgcgg gtcggcgacg
     2761 agctcggagg ccacatcgtg accggccacg tggacgggac ggcggaaatc gtcgccgcgg
     2821 cgcccgaagg cgaatcgatg cgcctgacct tcgaagctcc gcaggcgctc gcgcgcttca
     2881 tcgctccgaa gggttcgatc gcgctcgacg gcgtttcgct caccgtcaat gatgtcgacg
     2941 gcgtccgctt cggggtcaat ctcatcccgc acacgctcgc cgcaaccact ttcagcggcg
     3001 cgcgcaaggg ctctcgcgtc aatctcgaga tcgacgtgct ggcgcgctac gtggcgcggc
     3061 tggcgaaggg cctggcatga tgttccgcga ttacctttct tcgatggagg aattgatcga
     3121 ggacgcccgc aacgggcgca tgttcatcct ggtcgatgcg gaggaccgcg agaacgaagg
     3181 cgacctggtg attccggcgc agatgtgcac accgcaggcg gtgaacttca tggccaagca
     3241 cggccgcggg ctcatctgcc tcgcactcac ccaggagcgc gcgaacgcgc tgcgcattcc
     3301 gatgatgacg cagttcaatg gcacccccca aaaaacggcg ttcaccgtgt cgatagaggc
     3361 gaaggaagga atttcgaccg gcatatcggc ccacgaccgc gcccatacca tctcggtggc
     3421 gatcgacgcg accaagggtc ccgacgacat caccatgccc ggacacgtct ttcccctcgt
     3481 ggcacgccat ggcggttcgc tcgtgcgcgc ggggcacacg gaagccgccg tcgatatcgc
     3541 ccggctcgcg ggcctctacc cggcgggagt gatctgcgaa atcatgaacg acgacggcac
     3601 catggcgcgg ctgcccgatc tcgtgcagtt cgcgcaattg catggactga agatcggcac
     3661 gatcgccgaa ctcatcgcct atcgccgccg ctacgatcat ttcgtccagc gcacgatcga
     3721 aagcgatttt cacagccatt tcggcggact gtggaagttg atggtgtatg tcaacaccat
     3781 cgaatatgcg gagcacatcg cgctggtgaa gggcgatctc tccgggccga aaccggtgct
     3841 cgtgcgcatg catgcgctca acatcttcac cgacatgctg ggctacgagc cttatgagaa
     3901 ggaaatcctc gccgaatcca tgcgcatcgt ggcggggcag ggacgcggtg tcgtggtggt
     3961 tctcaggcag acgcgcccga ctttcgtgtc cgatctcctg tcgcggcgca tctccgacga
     4021 catcgaccgc cggcgggtaa aggaatatgg cgtcggggcg cagatccttc tcgatctggg
     4081 catatccaac atgatcctgc tgaccgacac ggcggagaaa aagatcgtgg cgctcgaggg
     4141 ttacggcctc aacatcgtcg gcacgcacgc gatccgcgga aacaggcagc ctggcgggaa
     4201 atcgtgacgc ccaacattct catcgtcgag gcgcgcttct attcgaatat ctccgacgcg
     4261 ctcctcgacg gcgcgacggc ggcgctcgat cgggcgggcg cctggtccga gcgcatcggc
     4321 gtgcccggag cactcgaaat tccgccggca atcctttttg cagccggggt gggcgaaggc
     4381 ggcggcaaga attacgacgg ctacatcgca ttgggctgcg tcatccgcgg cgatacctat
     4441 catttcgaga tcgtggcgaa ccagtctgcc cgcgggctga tggagctggg actgcgccac
     4501 ggcttgtgca tcggcaacgg catcctgact tgcgacacgg aagagcaggc catcgcccgc
     4561 gcttcgcgcg gcgaactcga caagggcgga gacgcggccc gcgcctgcct tgcgctcatc
     4621 gagacgcgca tgcgtctggg agcgacacga tgatctcgcc cgcgccacgc aacgaaatcg
     4681 cgagcggcgc acgcgccgag cgacgctcgg cgcggctggc ggcggttcag gcgctctacc
     4741 agatggaact gggcggcggc gacgcggaaa cggtggcgca ggaattcctc gatcaccgct
     4801 ttcagcgcga gcccgagctg gcagaagcgg gagcgcctga tgaggcgctg ttccgcgcgc
     4861 tccttgcggg cgtgccgcac caccaggcgg agatcgatcg cgccatcgcc tcgtgcctga
     4921 ctgcgagctg gcggctggag cgaatcgact ccattcttcg cgcgattttg cgtgcagcgg
     4981 cctgcgagct gatcgtccgg cacgacgttc cggcgcgtgt ggtgatcgac gaatatctcg
     5041 aaatcgcaca cggcttctgc ggcgaagagg aacgcacctt cgtgaatgca gttctcgatc
     5101 gcctggcacg ccgcaaacgc ccggccgagt tcggagaggc acctcccgat gaagaacccg
     5161 gagtttgaag cgaaggttcg tccgggcgaa ttcgagctga tcgcgaagct ctttgcaccc
     5221 ttatcgcgcg gcgtatccgg cgcgttcgcc ctcaccgacg atgtggcggt gatcgagcca
     5281 aagccaggtt gcgaatttgt tctcaaaacg gattcgctga tcgagagcgt gcattttctg
     5341 cgcagtgacc cagcaggaac ggtggcacag aaggcgttgc ggcgggcgct gtcggacctt
     5401 gccgcaaagg gcgcgggacc gtccgtctat cttcttgccc tcgctctccc atcgtggccc
     5461 gaattgaact ggcttgaagc tttcgcggcc ggtttggccg aggaccaggc gaaattcgaa
     5521 tgcgcacttg cgggcggtga gaccaacgca acgccgggtc ccgtgacggt caccgtcacg
     5581 gcggtgggat acgtgccgca aggggcgctg atccggcgca atggcgcgac gccgggcgac
     5641 agcgtgtttg tgagcggcac gatcggcgac gcaggcgcgg gacttgatct tctcgcgaac
     5701 ggcgacattc ccgcgagcgc ggcggcgcgg gatttcctcg tctcgcgcta tcggttgccc
     5761 atccccaggc tggagctcgg ccgcgaattg cgcggactcg cgagcgcggc acttgacgtc
     5821 tcggacggct tgctggcgga tctcggacat atcgccgaag cctcgcaggc gcgcatcgaa
     5881 atcgacgcgg cgcgcgtgcc gatgtcggat gccttgcgcg aattgagggg cgaggggata
     5941 tcagtgcgcg tgggcgccgc gatcgcggga gacgattacg agatcgcgtt tacggcgccg
     6001 cgctctgccg cggacacgat cctcgacgtt gcgcgccgaa cggggaccca ggtgacggaa
     6061 atcgggcgcg tggtttccgg cggcggcgtt gcgcttctcg atggaagcgg caatcccatt
     6121 caaatgccgc gcctgggtta cacgcatttc cgagcctaaa cgccggacac gggcctggtg
     6181 tcgcatgggc caacgcgccg ttgcctcccc aacgtccttt tggcatgttc ccccgctttt
     6241 cgcgccggca ctggccggcg cgctggcagg ggcgtccggc gccggccccg gcgacggtct
     6301 gcatagtttt ccggttacgc aaaggaacga acgatgggcc tgcagcttga actcatcctg
     6361 gccagtggtg tgctggcgct cctctatggc gcctggacga gccgctcggt catggccatg
     6421 agccctggca acgcgcgcat gcaggaaatc gccgcagcga tacaggaggg cgcggcggcc
     6481 tacctcaatc gtcagtacac gaccatcgcc atcgcgggcg tcgtgatcct catcattgcc
     6541 tttgcgatct tcggctggca ggtggcgctc ggctatctga tcggcgcgtc gttctcgggc
     6601 gttgcgggct atgtcggcat gaacgtgtcg gtgcgcgcca atgtgcgcac cgcggaggca
     6661 tcgcggcacg ggcttgctgc cggcctttcg atcgccttcc gctcgggtgc ggtgaccggg
     6721 ctgcttgtcg ccggactggg cctcatcggc gtcgccggct attacacgtt tcttcgctac
     6781 ggcctccatc tcgacatcaa tgacgaggca caagggcgtt tgatttccaa cgctctcgtc
     6841 gccctcggtt tcggcgcctc gctcatctct atcttcgcgc ggctcggcgg cggaatcttc
     6901 accaagggcg ccgatgtcgg tggcgacatg gtcggcaagg tcgaagcagg aattcccgag
     6961 gacgatccgc gcaatcccgc caccatcgcc gacaatgtcg gcgacaatgt cggggattgc
     7021 gccggcatgg cggcggacct gttcgagacc tatgcggtga cgaccgtcgc tacgatggtg
     7081 ctggcgtcga tctatttcac cgggggcttg cgcagcgcgc tcatgctgct gccgctcggc
     7141 atcgcagcca tgtgcatcgt tacatccatc atcggcacgt ttttcgtgca gctcggcaag
     7201 aacggctcga tcatgggcgc gctctacaag ggcgtgcttg cgacgggcgc gctgtcgctc
     7261 atcggcttgt ggccgctcac cgactggctc gtcggactcg acacggtgct cggccatgac
     7321 gtcaccggcc gttcgctgtt cttctgcggc gcggtcgggt tggccgtgac cggcgccatc
     7381 atctggatca cggagtatta caccggcagt gggttccgcc cggtaagggt gatcgccgag
     7441 gcgtcgcgta cgggtcacgg caccaatgtc atccagggtc tcgcgatctc gatggaatcg
     7501 acggcgctgc cggcgctcgt catctgcgtg ggcatcgtcg tcgcgaactg gctcgccggt
     7561 ctcttcggca tcgccatcgc ggtatccacc atgctgtcgc tcgccggcat gatcgtagcg
     7621 ctcgatgcgt tcggtccggt gacggacaat gccggcggca tcgcggaaat ggcgggtctc
     7681 gaaggcgatg tgcgcaagac gaccgatgcg ctcgatgcgg tcggcaatac caccaaggcc
     7741 gtcaccaagg gctacgcgat cggctcggcc gggctcggcg cgctggtgct cttcgccgcc
     7801 tacacccagg atctcaaata tttcatcgcc cattccgacg tatacggata tttccgcggc
     7861 ctcgccctgc cgactttctc gctctacgac ccgtgggtgg tggtgggact cttcatcggc
     7921 gggcttctac cctatctgtt cggcggcatc gccttgacgg cggtcggccg cgccgcgggc
     7981 gcggtggtgg aagaagtgcg caggcagttt cgcgagatgc cgggcatcat gcagggcacg
     8041 cagaagcccg attacggccg cgctgtcgac atgctgacga aggctgcaat ccgcgagatg
     8101 gtgattccct cgctcctgcc ggttctgtcg ccgatcgtgc tctatgtaat cgtcgacaac
     8161 atcgcgggaa aagcccaggc ttttgcagct ttgggggcga tgttgctggg cgtgatcgtc
     8221 accggcctgt tcgtggcgat ctcgatgacc tcaggcggcg gcgcctggga caacgccaag
     8281 aagttcatcg aggacggaca tttcggcggc aagggctccg acgcccacaa ggcggcggtc
     8341 accggcgaca cggtcggcga tccctacaag gacacggcgg gtccggcggt caatccgatg
     8401 atcaagatca ccaacatcgt ggcgcttctc ctgctcgcca cgctcgcgca cgggatttga
     8461 aaagcgggtt cacccctcac ccacctgagg agtggttgct cacccctcac ccgcgcttgg
     8521 gtggatttca ctacacgtat ctgcacgcct ctcccacaag gggagaggga attcggaatc
     8581 ctgcatcaac gatgttcgat cagcgcgcgg gattccctct ccccttgtgg gagagggact
     8641 acgcacaagc acgatcaacc gccgcaacgc gggtgaaggg tgagcaacca ctcaggcgga
     8701 ggtaaggcgc atgctgtcac ggataggg
//
LOCUS       DASZMC010000259         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_672681,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000259 DASZMC010000000
VERSION     DASZMC010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672681"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VG274_05265"
     CDS             complement(<1..555)
                     /locus_tag="VG274_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HWE06097.1"
                     /translation="MLGLAAAAPWLIGSPLLAQENPSRNRHAADFPPVPPNKKSLARV
                     ERVNHLAAECVRLDNEARDAFAQQHPGVQPELKLRLPKSTIHAFDWCNLNKVSEAHRQ
                     RTGDCWANSATESLECGFLIRDNLQVVLSTQPVLDHLKLHAKAISGSATMAFAYFLRI
                     GTALLQHYPYDGKPKEPLNVKLPYR"
     gene            1001..>2023
                     /locus_tag="VG274_05270"
     CDS             1001..>2023
                     /locus_tag="VG274_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWE06098.1"
                     /translation="MSAPTATRPRRFCLVLLKPSHYDDDGYVIQWLRSAIPSNSLAVL
                     YGLALGCAEERALGDDMEIEIHAMDETNTRIHPGRVASLVEGADAGVVMLVGVQSNQF
                     PRALDICAALRQRGIAVVIGGFHVSGTIAMLKERDPDVQRALDMGVSLFAGEAEGRLA
                     QVLRDARDQRLEPLYNFMQDLPDLTNAPLPILPADRVNRTAGSVTSLDAGRGCPYQCS
                     FCTIINVQGRKSRRRSPDDIEQIVRANIAQGLDSFFITDDNFARNRDWEAILDRLIRL
                     REVDKLKMSFIIQVDTQCHRIANFIDKAARAGVKRVFIGLENINPDNLFGAKKGQNKI
                     TEYRKML"
BASE COUNT          374 a          625 c          628 g          396 t
ORIGIN      
        1 ccgatagggc aactttacgt tcagcggttc tttgggcttc ccatcgtagg gataatgctg
       61 gagcagggcg gtgccgatgc gcagaaaata ggcgaaggcc atcgtcgcgc tgccgctaat
      121 tgccttggcg tgcagcttga gatggtcgag caccggctgg gtcgagagaa cgacttgcag
      181 attgtcgcgg atcaagaaac cgcattcgag cgattcggta gccgagttgg cccaacaatc
      241 gcccgtgcgt tgccgatggg cctccgaaac cttattcaaa ttgcaccagt cgaacgcatg
      301 gatcgtcgac ttcggcagcc gcagcttgag ctccggctgt acgccgggat gctgctgggc
      361 gaacgcgtcg cgggcttcgt tgtcgagccg aacgcactcg gccgccagat ggttgacgcg
      421 ctcgacccga gccagcgatt tcttgttggg cggaacaggc gggaagtcgg ccgcgtgccg
      481 gtttcgtgaa gggttctcct gcgccagcaa cgggctgccg atgagccacg gcgcggcagc
      541 cgccaggccc agcacccggc ggcgcgagca ccgtcggccg cacgcctcct caagccaagc
      601 gtgctgcatc tcgatcgtgg aacgcctcgt tcgattcgtc atgcgattta ccccgaaaac
      661 cgcccgttgg cgctgatttt gggccgcggg ccgatcccct cgcggacagg tcgctatttt
      721 agcagccgcc acggctagca gcacggtgcg tgcagatttc atccgttcgc cgcggcagta
      781 aagtccattc gtcgaacagc caggcgctct ccgctgcctg cgagtggacg ttttgtcgcg
      841 atgctagcag gtctttttgt tcgcaaaggt ctaggactgc cacgagttta ggcgtatctt
      901 ctgcgggctt tggtgcggca ggatgcgcgc gccaagcaag tttggccgtg atccgtccgg
      961 gtcttgaatg gattcagcag ctcggcgagg ttcaaaagca atgtctgctc ccaccgcaac
     1021 gcggccgcga cgattctgcc tggtgctgct caagccgtcg cattacgacg acgacggcta
     1081 tgtgattcag tggctgcgat cggcgatccc ctcgaattcg ctcgccgtgc tgtatggcct
     1141 ggcgctcggg tgcgccgaag agcgtgcgct gggcgacgac atggagatcg aaatccatgc
     1201 catggacgaa acgaacacgc gcatccatcc agggcgggtc gcgtcgctgg tggaaggcgc
     1261 cgacgctgga gtggtgatgc tcgtcggcgt gcagtcgaat cagtttccgc gagcgctgga
     1321 tatttgtgcc gcgctgcgcc aacgcggcat tgcagtcgtc atcggcggct tccacgtgtc
     1381 cggcacgatc gccatgctca aagagcgcga tcccgacgtg caacgcgcgc tggacatggg
     1441 cgtgtcgctg tttgccggcg aagccgaagg ccggctggct caggtgctgc gcgacgcgcg
     1501 cgaccagcgg ctcgagccgc tttacaattt catgcaggat ttgccggatc tcactaacgc
     1561 gccactaccg attctgccgg ccgatcgcgt caaccgcacc gccggctcgg tgacgagcct
     1621 cgacgccggc cgcggctgtc cttaccaatg ttccttctgc acgatcatca atgtgcaagg
     1681 ccgcaagtcg cgccgccgct cgcccgacga tatcgaacag atcgtgcgcg cgaacatcgc
     1741 acaaggcctc gattcgttct tcatcaccga cgataacttt gcccgcaacc gcgattggga
     1801 ggccattctc gatcggctca ttcggctgcg cgaagtcgac aagcttaaga tgagcttcat
     1861 catccaggtc gacacgcagt gtcatcgcat tgcaaacttc atcgacaaag ccgcccgtgc
     1921 cggcgtcaag cgggttttca tcgggctgga aaacatcaat cccgacaacc tgttcggcgc
     1981 caagaagggc cagaacaaga tcaccgaata ccgcaagatg ctg
//
LOCUS       DASZMC010000260         6783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4781008,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000260 DASZMC010000000
VERSION     DASZMC010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781008"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1161)
                     /locus_tag="VG274_05275"
     CDS             complement(55..1161)
                     /locus_tag="VG274_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWE06099.1"
                     /translation="MQSFWRTAAMTLSLVPGPGSAVSAAEMPRYAHIFVIIGENKGFD
                     QMMRDRTLTPQLHRLAGEYGQATQFFAEDHPSEGNYVAMVGGDTFGIKDDDAFFCKPG
                     LKNEFCEKSGRADYVDHSITARSLPDQLAEKGLSWKAYLEDIPAAGALVPRWPTRDYA
                     IRGLPLELYAAKHNGFVNFKSVREEPYPELMTRFVGFAQLDADLASGDMPNYAHIVPD
                     LCNDMHGLSRREDPNVPEDCGTDTGLIRRGDAEIGELVAKFLRSKVWSAPGNAAIVVT
                     FDENDDEERQTGPQGCCGTDPKSVANFGGGRIPTLVITNHGPRHFVDPTPYNHYSLLR
                     TTEEAFGIHEYLAHAADRAKGVVTMAPLFAVTAE"
     gene            complement(1380..1934)
                     /locus_tag="VG274_05280"
     CDS             complement(1380..1934)
                     /locus_tag="VG274_05280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWE06100.1"
                     /translation="MPLSQLDPVAAQIVIDMQKGIVALPTVHPIGGVIANVAKLAGAF
                     RGRNLPVVLVNVTGRAPGRAETAFNFSPPPDWAELIPELDVQTSDYRVTKENVGAFYG
                     TALERILRRRGVTQIFLTGVATGSGVEATARAAYDRGYNVVPVTDAMTDLDPDMHRHA
                     VEKVFPKIGERTTTADALAMLKSG"
     gene            complement(2187..2381)
                     /locus_tag="VG274_05285"
     CDS             complement(2187..2381)
                     /locus_tag="VG274_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06101.1"
                     /translation="MHGVARALLGALSPSLVGVRYPVQMLPLLLFELMWKSIRLLAIA
                     LRLRLAHRIDRIRRTRCSPA"
     gene            2403..2756
                     /locus_tag="VG274_05290"
     CDS             2403..2756
                     /locus_tag="VG274_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWE06102.1"
                     /translation="MYDGWPDFGRQNFVFRYRKFLKPGGVFAATDLGPWAQNVVLALW
                     SSIARSNGVVIPVPGRIDGFVDFLKRRMEADQFRAIIDRKYPLAAIADAYRYVETGQK
                     AGIVVIDVAVADESL"
     gene            2778..3446
                     /locus_tag="VG274_05295"
     CDS             2778..3446
                     /locus_tag="VG274_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06103.1"
                     /translation="MLAPKAFVSWSSGKDSAFALYEVRRLGIAQIVGVLTTINEVYDR
                     VAMHGVRSALLDRQIAALGLPAIKVPLPSPCPNEIYEARMADAFERIRSQGVRHMIFG
                     DLFLEDIRAYREKQLAPAGMQGLFPLWQRDTRELAEQMISVGIEAHLVCVDPRHLHRR
                     YAGRKFDGDLLRDLPEGVDPCGENGEFHTVVSAGPMFSAPIPVCVGEIVERDGFVFAD
                     VIPG"
     gene            3592..4059
                     /locus_tag="VG274_05300"
     CDS             3592..4059
                     /locus_tag="VG274_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06104.1"
                     /translation="MRKMISLIFAAGLLASGFAAAAETIGTVHVGGRHGGGGGRSGNG
                     HVVRGGGGGNRAGHLRNGGGIRYGVGVGLGVPYYAGDPGVDSYGGYADSGDNAAPADP
                     DPGAYGSDDAPADAPVSPVLYYCDNPQGYYPDVTTCNDDWQPVQAAPPADSGN"
     gene            complement(4282..>6783)
                     /locus_tag="VG274_05305"
     CDS             complement(4282..>6783)
                     /locus_tag="VG274_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWE06105.1"
                     /translation="MFTDLHVKTIVFLQFSVGDTAVYTARLTTKHAIFPGQFWSGDAF
                     PSEIAYDDVQETLTAPGNLALHVENHGVGFEKSQKDGQTVYRWHYNAPRSAATEPSVF
                     ALAQLPHFFVSTFADYPALARAYAEAAAPETAVTPAIKALADNITKGVHGRREEVRAL
                     YQWVAQHIRYVAVELGKGTLIPHAADTVLSNGYGDCKDHVALLGALLAARGIANEAVL
                     LNATAIYRLFDVATFAGLNHVITYVPALNLFLDSTPGVAPFGVLPFSEYGKPAVYASE
                     TAPHLGSIPVLTAGLAELTTKTVAHLGKDGVLSGTTTTTATGPYSVQLRLVGLGIQAL
                     GPEASAKRLLSALGYGGSASGTLEAPSPAEVSDTYVVNGSFTNPAWSSQLAGTEHFYL
                     PGGMRLLGASGDGLMGSFASANAKPDTEIPCYSGQASEDLSLETPPGAQFSNVPADTH
                     VNTANIRFDAHWTLTNGTLSVHRHFTSLIDRPLCTVAIRAANAEALKTISDSYNVQLS
                     FESPGRFAQQPLHASDIANPPNDPKLAAMLEDAFAAMQHHKDDDAIAKFSTVLAQPDV
                     PIAASYPSHYNLAVLHTRRWNYDAALADLNAGLTAMPGDSQMLVTRAHVYFSSADFSR
                     ALADCESELGQHPNDAAALQLRANIAMETGRYQDAIRDYTGELQLVRNPNDLVLRAVA
                     YHRMERESDAAADIAQAAQLGDAAAKADYDEITGATRSAGMAGDATGLATAVSETAWR
                     SAAPGLSAPTPSNAHTSRYPPLSRRLGEMGRVTVEFVIGLDGSAADPKVEKSSGFPAL
                     DASALESMKAWRYHPALDKGNPVPTRSHVQVGWVL"
BASE COUNT         1191 a         2063 c         2217 g         1312 t
ORIGIN      
        1 gggcgattga tgatacgccc aacatggtat acatgccaac cgcattggtg atacttactc
       61 cgccgtcacc gcaaacagcg gcgccatcgt caccaccccc ttcgcgcggt cggcggcatg
      121 ggcgaggtat tcgtggatgc cgaatgcctc ctcggtcgtg cgcagcagag aatagtggtt
      181 gtacggcgtc gggtcgacga agtgccgcgg cccgtgattg gtgatgacca gcgtcggaat
      241 gcgtccgccg ccgaaattgg cgacgctttt gggatcggtg ccgcaacagc cctgcggccc
      301 ggtctgccgc tcttcgtcgt cgttctcgtc gaaggtgacg acgatcgccg cgttgccggg
      361 tgcgctccac actttcgagc gcaggaactt cgcgaccagc tcgccgatct ccgcatcgcc
      421 gcgccggatg agaccggtgt ccgtgccgca atcctcggga acattgggat cctcgcgcct
      481 cgagagcccg tgcatgtcgt tgcacaggtc gggaacgatg tgggcgtaat tcggcatgtc
      541 gccggaggcg agatccgcat caagctgcgc gaaacccacg aagcgagtca tcagttccgg
      601 atagggctct tcgcgcacgg atttgaaatt gacgaagcca ttgtgcttgg ccgcatagag
      661 ctccagcggc aggcccctga ttgcatagtc gcgtgtcggc cagcgcggca cgagcgcgcc
      721 cgcggcggga atgtcttcga gataggcctt ccagctgagc cccttctccg ccagctgatc
      781 gggcaggctg cgcgcggtga tgctgtggtc cacataatcc gcgcggccgc tcttctcgca
      841 gaattcgttc ttcagccccg gcttgcagaa gaaggcgtcg tcgtccttga tgccgaacgt
      901 gtcgccgccc accatggcga cgtagttgcc ttcgctggga tgatcctcgg cgaagaactg
      961 cgtggcctgg ccgtattcgc cggccagccg gtgcagctgc ggcgtcagcg tccggtcgcg
     1021 catcatctga tcgaaaccct tgttttcccc gatgatgacg aagatatgcg cgtagcgcgg
     1081 catctccgcc gcactcaccg cactaccagg tccaggaacg agcgacagcg tcattgcggc
     1141 cgtacgccag aaagattgca tcggcagttc tcctctagtg ccaagattca aagacaagga
     1201 cagatttaac gcagaggtcg cagaggatac gcagaggacg cagaggaatt tcttgcgcgc
     1261 ttcgcgcgcg atcgtcttcc tgcgtccact gcgctacccc tgcgtcctct gcgttaaaaa
     1321 tctgtcggtg cacgattcgc ccggcttgtt aggaatcaac tcggcaaccg gtcggtgctt
     1381 caccctgatt tcaacatcgc gagcgcgtcc gcggtagtgg tgcgttcgcc gattttcggg
     1441 aacaccttct cgaccgcgtg gcggtgcata tcgggatcga ggtccgtcat cgcgtcggtg
     1501 accgggacga cattgtatcc ccggtcgtag gcagcccgcg ccgttgcctc gacgccgctg
     1561 ccggtcgcga cgcccgtgag gaagatttgc gtcacgccgc gccggcgcag gatccgctcc
     1621 aatgccgttc cgtaaaaggc gccgacattc tccttggtga ccctgtaatc gctcgtctgc
     1681 acgtcgagtt cggggatgag ctcggcccag tcgggcggcg gcgagaagtt gaatgccgtc
     1741 tcggcgcggc ccggcgctct gcccgtcacg ttgaccagaa ccaccggcag gttgcgcccg
     1801 cggaacgcgc ccgcgagctt cgcgacattg gcgatgacgc cgccaatggg atgcaccgtc
     1861 ggcagagcga cgatgccctt ctgcatgtcg atgacgatct gggcggcgac gggatcaagc
     1921 tgggagagcg gcatgtgcgg tcctttgctg ctggtgcgac ggcagtatgg ccgaaatcgc
     1981 gcgcagaggc gcggaacgct gcaataatgc aatccgtact ccccgggacc gcgggcctct
     2041 ggcctgcatt cgcagcggat cgcgcgggcg ggtgcgcgcg ggacgcgcgg tcccagtggc
     2101 tcaccgccag cggtctccgg gctttctgat gtggttcgcg aacacgtaac gccgggggac
     2161 aacagtcgga accaggacga ctcccgtcag gcgggtgaac accgcgtccg ccgtatccgg
     2221 tcgatccggt gggcgagccg cagccgcagc gcgattgcga gcagccggat cgatttccac
     2281 atgagctcga agagcagcag aggcagcatc tgtactggat atcgaactcc cacaagtgaa
     2341 ggcgacaacg cgcccagcag ggcgcgggct acgccgtgca tcacgcctgt gacgggtgcg
     2401 tggtgtatga tggctggcca gatttcggtc ggcaaaactt cgttttccgg taccggaaat
     2461 tcttgaagcc tggcggcgtc tttgctgcga cggatctcgg accctgggcg cagaatgtgg
     2521 tgctcgcgct gtggtcgtcg atcgcacgga gcaacggggt cgtcattccg gtgccggggc
     2581 gcattgacgg gtttgtcgat ttcttgaagc gccgcatgga ggcagatcaa tttcgtgcga
     2641 tcatcgatcg gaaatatccc ctcgcggcaa ttgcggacgc ctaccgttat gtcgagacag
     2701 ggcaaaaggc cggcattgtc gtgatcgatg tcgcggtggc cgacgagagc ttgtaactgc
     2761 gaaagagagc aaatcgcttg ctggcgccga aggcatttgt ttcctggagt tcgggcaagg
     2821 acagcgcctt cgcgctctac gaagtgcggc gccttggcat cgcgcaaatt gtcggcgtcc
     2881 tgacgacgat caacgaagtc tacgaccggg tcgcgatgca cggcgtgcgc agcgcgctgc
     2941 tcgaccggca aatcgcagca ctcggcctgc ccgccatcaa ggtgccattg ccgagcccat
     3001 gtcccaatga aatctatgag gcgaggatgg ctgacgcctt cgagcgcatc aggtcgcagg
     3061 gagttcgcca catgattttc ggcgacctgt tcctcgagga tatccgcgcg tatcgggaaa
     3121 agcagctcgc accagcggga atgcaggggt tgttcccgct ctggcagcgc gatacgcgcg
     3181 agctggcgga gcagatgatt tccgttggca tcgaggcgca tctcgtatgc gtcgaccccc
     3241 ggcatctcca ccgccgctac gcggggcgga aattcgacgg cgatcttttg cgcgaccttc
     3301 ccgaaggcgt cgatccgtgc ggcgagaacg gagagtttca taccgtcgtg tcggccggtc
     3361 ccatgttttc cgcaccaatt cccgtgtgcg tcggcgaaat tgtcgagcgt gacggcttcg
     3421 tctttgcgga cgtcattccc ggataggcag cgcccgcgct tgcctccctt cttcacatca
     3481 tgtcgcgaat tgtcgccgtt caccgcaaaa actggccgta cgcattgtaa aggccgagcg
     3541 gcggcatagc ttgataacgg gaacattgag ttctgtgaac gaggccgatc aatgcgaaag
     3601 atgatctcgc tgattttcgc cgccgggctg cttgcttccg gttttgccgc agccgcggag
     3661 acgattggca ctgtacatgt cggcggccgg cacggcggcg gcggtggacg ttccggtaac
     3721 ggccacgtcg tgcgcggcgg cggcggcggg aaccgcgcgg gacatttgag aaacggcggc
     3781 ggaattcgct atggcgttgg tgtcgggctc ggtgtgccgt actacgccgg cgatcccggt
     3841 gttgactcct atggcggata tgccgattcc ggcgataatg ctgcgccagc cgatccggat
     3901 ccgggagctt acggctcaga cgatgcgccc gccgatgcgc ccgtgtcgcc ggtattgtat
     3961 tactgcgaca atccgcaggg ctactacccc gatgtcacga cctgcaacga cgattggcag
     4021 cccgtccagg ccgccccgcc ggcggattcc ggtaactaaa gccgtttcag attgaatttg
     4081 cgcaagcaat attcccgcgc gtgttgcggt gattatcggg ttgattctcc ttacgcccaa
     4141 aacgttttcc tcccccacga tgggggagga aaatcaagcg cgcagcattg ctgggattcc
     4201 aggccgaagg gctcgggcgc attcgcacgc gaccggcgca gtacatcgag ttcttgtcgc
     4261 cggcgctgtc tccattcgcg gctacagcac ccacccgact tgaacgtggg agcgcgtggg
     4321 gaccgggttg cccttgtcga gcgccgggtg gtagcgccac gccttcatcg attcgagcgc
     4381 ggaagcatcg agcgccggaa agccgctcga tttttcgacc tttggatcgg ccgcgctgcc
     4441 gtcgagaccg attacgaatt ccaccgtcac acgtcccatt tctcccagtc gccgcgagag
     4501 cggcggatag cgcgatgtgt gcgcgttgga tggcgttggt gcgctgagcc ccggcgcagc
     4561 agaacgccag gccgtctccg aaaccgctgt ggctaatccg gttgcgtctc cggccattcc
     4621 cgcggatcgg gtggctccgg tgatctcgtc gtaatccgcc ttcgccgcgg cgtcgccgag
     4681 ctgcgcggcc tgcgcgatat cggcagcggc atcgctctcg cgctccatcc gatgataggc
     4741 gactgcgcgc agcaccagat cgtttgggtt gcggacgagc tgcaattcgc cggtgtagtc
     4801 tcgaattgcg tcctggtatc tcccggtttc catcgcgatg ttcgcgcgga gctggagagc
     4861 agccgcgtcg ttcggatgct gacccagctc gctctcgcaa tcggcaagcg cgcgggagaa
     4921 gtcagcgctg gagaaataga catgggcccg cgtgaccagc atctgcgagt cccccggcat
     4981 tgccgtcagc ccggcgttga ggtccgccag cgccgcgtcg taattccaac gacgcgtatg
     5041 gagcactgcg aggttgtagt gcgagggata ggacgccgcg atcggaacat ctggctgcgc
     5101 gagaacggtc gagaacttcg caatggcgtc gtcgtcctta tggtgctgca tggctgcgaa
     5161 cgcgtcttcc agcatggcgg caagcttcgg atcgtttggc gggttggcga tgtcggatgc
     5221 atgcaagggc tgctgcgcga accttcccgg cgattcgaag gaaagctgaa cattgtagct
     5281 gtcggagatc gtcttcagcg cctcggcgtt cgcggcgcga atggccaccg tgcaaagcgg
     5341 ccggtcgatc aggcttgtaa agtgccgatg tacgctcaag gtcccgttcg tgagagtcca
     5401 atgcgcgtcg aagcggatgt tcgccgtatt gacgtgcgtg tccgccggca cgttcgagaa
     5461 ctgcgcgccc ggcggcgtct cgagtgagag atcctcgctc gcctggccac tgtagcaggg
     5521 aatctcggtg tcgggtttcg cattggcgct ggcgaacgat cccatcaatc cgtcgcccga
     5581 ggcacccaga aggcgcatgc cgccaggaag atagaaatgc tccgtcccgg cgagctggct
     5641 tgaccaggcg gggttggtga acgagccgtt caccacatag gtatccgaaa cctcggccgg
     5701 tgaaggggct tccagcgtac cgctcgcgct gccgccatat ccaagggctg acagaaggcg
     5761 cttggcggag gcttccgggc cgagcgcctg gattcccagg cctaccagcc gcagctgaac
     5821 ggaatagggt cccgtcgccg tcgttgtcgt cgtgccggac agcacgccgt ccttgccgag
     5881 atgtgcgaca gtcttggttg tcaactcggc gagacccgct gtcagcacgg gaatcgaccc
     5941 caggtgcggc gccgtttcgg acgcatagac ggcgggcttg ccatattcgg aaaacggcag
     6001 cacgccgaac ggcgccacgc cgggcgtgct gtcgaggaag agattgagcg ccggcacgta
     6061 ggtgatgaca tggttgagtc cggcgaaggt cgccacgtca aacaggcggt aaatcgcggt
     6121 cgcgttcagc agcaccgcct cgttcgcgat gcctctggcc gcgagaagag ccccgagcag
     6181 cgcgacgtga tccttgcagt cgccataacc gtttgacagc acggtgtccg cggcatgagg
     6241 gatgagcgtg cctttgccga gttcgacggc aacatagcgg atatgctggg cgacccattg
     6301 gtaaagcgcg cggacttctt cgcggcggcc atgcacgcct ttggtgatgt tatcggcaag
     6361 cgccttgatt gcgggtgtca cggccgtctc gggcgccgcc gcctctgcat aggcgcgcgc
     6421 cagcgcggga tagtccgcga aggttgaaac gaagaagtgc ggcagttgtg cgagcgcaaa
     6481 gacggaaggc tccgtcgcgg cggaacgggg cgcgttgtaa tgccagcgat agaccgtctg
     6541 cccgtccttc tgcgatttct cgaagcccac gccgtgattt tcgacatgga gcgcgagatt
     6601 gcccggcgcc gtgagcgttt cctgcacgtc gtcgtacgcg atctcgctgg ggaacgcgtc
     6661 gccgctccag aactgcccgg gaaagatcgc atgtttcgtt gtcaggcgcg ctgtgtagac
     6721 ggcggtatcg cccaccgaga attgcagaaa gacgatggtc ttcacatgaa ggtcggtaaa
     6781 cat
//
LOCUS       DASZMC010000261         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2027500,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000261 DASZMC010000000
VERSION     DASZMC010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027500"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VG274_05310"
     CDS             complement(<1..302)
                     /locus_tag="VG274_05310"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HWE06106.1"
                     /translation="MSRIHTTFFIAALAAVSLDAAPITPPSGFSQADRADLERLSNAL
                     NAIHTLEGTFVQIGPEGQVDQGKFFIQKPGRMRFEYTPPNPTLVVSDGRWVAVENT"
     gene            complement(312..932)
                     /locus_tag="VG274_05315"
     CDS             complement(312..932)
                     /locus_tag="VG274_05315"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWE06107.1"
                     /translation="MAEKNTIRCQIVEAAKKRFSHFGYAKTTMAEVAADCSMSPGNLY
                     RFFPGKLDIAESIATEDYEKHLEALRKLAVAPNRTARERLHDLLFEELRRTYHKLEKD
                     PRAYEMATVIAHERPQYANWMLANERKILVQLFDEAEQRGEFAIDDKEITAEMIQSAT
                     MKFRYPQLWSKLTLPRLERELDGVLKLLVNGLCPGRTSKAGSEAAA"
     gene            1046..2053
                     /locus_tag="VG274_05320"
     CDS             1046..2053
                     /locus_tag="VG274_05320"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWE06108.1"
                     /translation="MTKNGQDAEPYRHIGILGAGAWGTALAVVAARAGRSVTLWARDP
                     TLAESMALQRENARFLSGVELPSGIELTIERGAVSEVDAVLVVVPAQQTREVLTGFRP
                     RSAAPLVLCMKGIERGSGKLMTEILAETVPGAEPAILSGPSFARDVAQGLPTAVTIAA
                     HAPVARRLQASLGQMAFRPYASEDLVGVALGGAAKNVYAIGCGIVAGLGLGESARAAM
                     LARGFAELRRLGRALGGRAETLMGLSGLGDLVLTATSPTSRNFSFGLALGRGKTLSEL
                     RAPGAQLAEGVETAPALVGRARRLAIELPIAQTLADILDGEIAPGDALPRLMSRPLRM
                     E"
     gene            2060..2353
                     /locus_tag="VG274_05325"
     CDS             2060..2353
                     /locus_tag="VG274_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWE06109.1"
                     /translation="MLFVITAMDKEDSLALRMATRAAHFDYATATGVVKLGGPFLDAK
                     GDMIGSLIIIEAADMGQARSWQENDPYAKAGLFSHVELRPWKATANHCKADFR"
     gene            2350..2772
                     /locus_tag="VG274_05330"
     CDS             2350..2772
                     /locus_tag="VG274_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWE06110.1"
                     /translation="MKYWLFKSEPESWSWDRQKAKGAKGEPWSGVRNHQAANNMKAMK
                     NGDRGFFYHSGEERRIVGIVEVIGEYRPDPTDEGGRFGLVDVKAVADLPKPVTLEQAK
                     HDPKLADMVLVKFSRLSVQPVGNAEWKYICRLGGLKNH"
BASE COUNT          512 a          881 c          947 g          579 t
ORIGIN      
        1 ttcgtatttt cgacggcgac ccatcttccg tcgctgacga ccagcgtcgg attggggggc
       61 gtgtattcga atcgcatccg tccgggcttc tggatgaaga atttgccctg atcgacctgc
      121 ccttccgggc cgatctgcac aaacgttcct tcgagggtgt gaatcgcgtt gagcgcattg
      181 ctgagccgct cgagatcggc ccggtctgcc tggctgaatc cgctgggcgg cgtgatcggc
      241 gcagcatcca gggaaacggc cgccagggcc gctataaaaa acgttgtgtg aatacgactc
      301 atgcgaacgg ttcaggccgc cgcttcgctg ccggccttgg atgtccggcc ggggcacaga
      361 ccattgacca atagcttgag cacgccgtcg agttcgcgct cgagcctggg aagcgtgagc
      421 ttcgaccaca gctgcggata acggaacttc atcgtcgcag actggatcat ttcggcggta
      481 atttccttgt cgtcgatcgc aaattcgccg cgctgctcgg cctcgtcgaa cagttggacc
      541 agtatctttc gctcgttcgc cagcatccag ttggcatatt gcggccgctc gtgcgcgatc
      601 accgtcgcca tctcgtaggc tcgcgggtct ttttccagct tgtgataggt acggcgcaat
      661 tcctcgaaca gaagatcgtg caggcgctcg cgggcggtgc ggttcggcgc gacggcgagt
      721 tttcgcaacg cctccagatg tttttcgtag tcttcggtgg cgatcgattc ggcgatgtcg
      781 agctttcccg gaaagaagcg gtagagattg cccggcgaca tgctgcaatc cgccgcaact
      841 tccgccatgg tggttttcgc gtacccgaaa tgcgaaaacc gctttttcgc cgcctcgaca
      901 atctggcagc gtatggtgtt tttttcggcc atggctcgtt ccgcttcccg gacgtttttt
      961 tcttgttcca tccaaacata gtggcatttt ctgaataatc aacaatccgt tcaatcttcg
     1021 caacccggcg gcgtcaagcg atcgaatgac caaaaacggc caggacgcag agccttaccg
     1081 gcatatcggc atcctcggcg ccggcgcctg gggtacggcc cttgcggtgg tcgcggcccg
     1141 cgcggggcgc tccgtcactc tgtgggcgcg cgacccaacg cttgccgaat ccatggcatt
     1201 gcaacgcgag aacgcgcgct tcctttccgg ggtcgaactg ccttccggca tcgaattgac
     1261 catcgagcgc ggcgcggtct cggaagtgga tgcggttctt gtggtggttc cggctcaaca
     1321 gacgcgcgaa gtccttaccg ggtttcgtcc gcgctccgcc gcgccgctcg ttttgtgcat
     1381 gaaaggcatc gagcgcggaa gcggcaaact catgaccgaa atattggccg aaacggttcc
     1441 cggcgcggag ccggcaattc tttccggccc atccttcgcg cgtgacgtgg cccagggatt
     1501 gccgacggcg gtgacgatcg cggcgcatgc gccggtggcg cgccgcctgc aggcgagcct
     1561 gggccagatg gcctttcgcc cttatgccag cgaggatttg gtcggcgtgg cgctgggcgg
     1621 agcggcgaaa aacgtttacg cgatcggatg cgggatcgtc gccggacttg ggctcggcga
     1681 aagtgcgcgg gccgcgatgc tggcgcgcgg ttttgcggaa ttgcgccggc tcggccgcgc
     1741 cctcggcggc cgggcggaga ccttgatggg cctttcgggt ctgggggatc tggtgctcac
     1801 cgcaaccagt ccgacctcac gcaatttctc gttcggcctc gcgctcgggc gcgggaaaac
     1861 tctttccgag ttgcgcgctc cgggggccca gctggccgaa ggcgtcgaga cggcgccggc
     1921 cctcgtcggg cgggcccggc gtctcgcgat cgaattgccc attgcccaga cgctcgccga
     1981 tattctcgat ggagagatcg cgccgggcga tgcactgccg cggctgatgt cgcgtccgct
     2041 caggatggag tgaattcgca tgctgttcgt catcacggcc atggacaagg aagactcgct
     2101 cgcattgcgc atggcgacgc gtgcggcaca tttcgattac gcgacggcga ccggcgtggt
     2161 gaaactgggc ggcccgttcc tcgatgcgaa gggggacatg attggcagtc tcatcatcat
     2221 cgaagcggcg gacatgggcc aggctcgaag ctggcaggaa aacgatccct acgccaaagc
     2281 cgggctgttc tcgcacgtcg aactccgccc ctggaaagcc acggccaatc actgcaaggc
     2341 ggatttccga tgaagtactg gctgttcaag agcgagcccg agagctggtc gtgggaccgg
     2401 cagaaggcaa agggcgcgaa gggcgaaccg tggagcgggg tgcgcaatca ccaggcggcc
     2461 aacaacatga aagcgatgaa gaatggcgac cgggggttct tctatcactc cggcgaggag
     2521 cgccgcatcg tcggcattgt cgaagtgatc ggcgagtatc gccccgatcc caccgatgaa
     2581 ggtggccgct tcggattggt ggacgtgaag gctgtcgccg atctgcccaa gcccgtcacg
     2641 ctggaacagg caaagcacga tcccaaactc gcggacatgg tgctggtgaa gttttcgagg
     2701 ctttccgttc agcccgtcgg caatgcggaa tggaagtaca tctgccggct gggcggattg
     2761 aagaatcact gaggtccgcg cgtggagcgc ggcgatgagc gcggctcgct gaccaacacg
     2821 gccggcgcgc tctactactt cgccaaccag gcggccggcg tgaccaacga gctggtttcg
     2881 gtaaacacga cgtggcggac ctatctgacc ccctacggc
//
LOCUS       DASZMC010000262         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1496628,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000262 DASZMC010000000
VERSION     DASZMC010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496628"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VG274_05335"
     CDS             <1..580
                     /locus_tag="VG274_05335"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWE06111.1"
                     /translation="LGGGSTDAAAALRLLAMHNRIALDDDRLIAAARATGADVPVCLD
                     PQPRLMRGVGELLSEPLRLPGLPALLINPRVSVATKDVFTALRAPPLAQSKPSASADA
                     GWAGGGRRELIDFVARHGNDLEPPAIALHPAIAQALAALRALAGCRLARMSGSGASCF
                     GLFDSNRAAAAAARALRVEHPQWWIRPTVLGG"
     gene            718..802
                     /locus_tag="VG274_05340"
     tRNA            718..802
                     /locus_tag="VG274_05340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:752..754,aa:Leu,seq:caa)
     gene            1304..>1583
                     /locus_tag="VG274_05345"
     CDS             1304..>1583
                     /locus_tag="VG274_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWE06112.1"
                     /translation="MMHGHEKSDSVIVARKSPNKTGQPAAEVMERRTEAKENAKQQRM
                     RRAQNRESVKQALDRVRNAARQRKKERFTTLFHHLTIDMLRLSFLALKR"
BASE COUNT          299 a          492 c          543 g          249 t
ORIGIN      
        1 tctgggcggc ggctcgaccg atgcggccgc ggcgctgcgg ctcctcgcca tgcacaaccg
       61 catcgcgctc gacgatgatc gcctgatcgc ggcggcacgc gcgaccggcg ccgatgtccc
      121 ggtgtgcctc gatccgcagc cgcggctgat gcgcggggtc ggcgaactgc tgtcggagcc
      181 gttgcggctt cccggcctgc cggcgctcct gatcaatccg cgcgtgagcg tcgccaccaa
      241 ggacgtgttc acggcgctgc gcgcgccgcc gctcgcgcaa tccaagccgt ccgcctcggc
      301 ggacgccgga tgggccggag gcggcaggcg cgagctgatc gacttcgtgg cgcgccacgg
      361 caacgatctc gagccgcccg cgattgcgct ccatcccgcc atcgcgcaag cgctggcggc
      421 gctgcgcgcg ctcgccggct gccggctcgc ccgcatgtcc ggctcgggcg ccagctgctt
      481 cggcttgttt gattccaatc gcgccgccgc tgccgccgcg cgcgctttgc gcgtggagca
      541 tccgcaatgg tggatcaggc cgacggtgct gggcggatag gcaccggctc cgggcgtgag
      601 acgtgtttgg agcgaaaccg tccgtgaaaa ttgggctcac ggcagacgcg cccgactcaa
      661 aagacaggct tgggttgggg ggatgatccc taaccgcgat ctcgccagca ggcgcgtgcc
      721 cgtgtggcgg aactggtaga cgcgcgcgat tcaaaatcgc gttctgcaag gagtgggggt
      781 tcgattccct ccacgggcac caccaaggcc ttccatcgtt gttcgccgtg caccatttca
      841 gaatcaaatc aatcgcttaa ccgttgcgac tcacaagagg gccgcgcaag cgatcctctc
      901 tttctgcata tcggctcagc aacgatttcg acataatggg gtttatggtg cgtcaggaga
      961 agctggcgcg ttccagccgg tgaaagaccg gcacgggtag ggcagtagcg cgccgcccgg
     1021 tagcgagtgt tgcgtggtcg tcggcgacgg cggctgcgaa gcgtacacag cgatctgctg
     1081 gggtgtggga ttgagcttcg aaagccataa aatcgcagag gccgaggcag tcataagtgc
     1141 cgaaggcaac atgtgcggca ccgccaagcg aggtgctgac gctctgccgg agtcggagac
     1201 cacatcacgg cggaaaggaa cgcgtcggaa cctgagaggt ctcacgtctg gccaccggca
     1261 agagccgagt ggtccgcatc gggaaggtga ggaaccgaag ccgatgatgc acgggcatga
     1321 gaaatcagac tccgtcatag tagcgaggaa gtcgccgaac aaaactgggc aaccggcagc
     1381 ggaggtgatg gagcgaagga cggaggccaa ggagaacgcg aaacagcaac gcatgcgccg
     1441 ggctcagaac cgggaaagcg tgaaacaggc gctggatcgc gtacggaacg ccgcaaggca
     1501 acggaagaag gaacggttca ccacgctctt ccaccacctc acgatcgaca tgctgcggct
     1561 atcgtttctc gcgcttaagc ggg
//
LOCUS       DASZMC010000263         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1762720,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000263 DASZMC010000000
VERSION     DASZMC010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762720"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VG274_05350"
     CDS             complement(<1..813)
                     /locus_tag="VG274_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HWE06113.1"
                     /translation="MTIPFRDNDSAIQAAIASAHYPSLAAALVHLTGDASLIRGGIKP
                     VYEFFGDGQGGLSDHQKSEIAEAAVKAIGALRDGRLARTTSPPVSTIRETMNFVAGAK
                     IPDRYLPFLVEELALEGQDRIGDRLEDIEADRKQAFRVLIIGAGMSGILAAIRLGQAG
                     IPYTIVEKNDEVGGTWFENAYPGCRVDNPNHMYSYSFAPNHDWPHHYSTQDALFAYFR
                     DVAERYGVRARVRFRTEVIGSAYDERRNIWVSRARSADGREETIESNAVITAV"
     gene            1004..>2345
                     /locus_tag="VG274_05355"
     CDS             1004..>2345
                     /locus_tag="VG274_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HWE06114.1"
                     /translation="MIAFLRWLRDIALGAVNGVVRLAVLIVVLAAVLIVIGLIEGDGM
                     PPRMVLNLDLRSSPPDSSNATSLFFDRRTPPVMDTVLALDRAGRDSRVKGAFVELGSG
                     GLSVAQGEEIGAALIRFRATGRFVIAYAQGFNSTGLGDYLTAASADQIWMEPRSPFSA
                     AGSGAGAIFLRGLFDKIQAVPQIVKRADYKSAADMYMQKGYTQPDREQTTAFLQSWYN
                     SAVAAVSAERKLDRRAVIASFQASPQFAEDAQRARLIDRLGYDDQAKDAARARANRAK
                     LVAIGEYARATESEGAVGSGPHVALIEAAGEIVDGTADHGALGGGTVIAGDDYADAFR
                     RATRDRSIKAILFRIDSPGGSVTASDQILEALREAKAAGKPVIVSMGPLAASGGYYVS
                     CAADQIVAEPATLTGSIGVLTGKVAMGKSLGMIGVGADEIGVGKNALFDSGIEPY"
BASE COUNT          417 a          715 c          769 g          444 t
ORIGIN      
        1 gacagcggtg atgacggcgt tgctctcgat tgtctcttcg cgaccgtccg ccgaccgcgc
       61 gcgcgaaacc cagatattgc ggcgctcatc atatgccgac ccgatgacct cggtgcggaa
      121 gcggactcgt gcgcgcacac cgtatcgctc ggcgacatcc cggaaatagg cgaacagcgc
      181 atcctgggtt gaataatggt gcggccagtc gtgattggga gcgaaggagt aggaatacat
      241 gtgattggga ttgtcgacgc ggcaaccggg ataggcgttc tcgaaccatg tccctccgac
      301 ttcgtcattc ttctcgacga tcgtgtaggg gatgccagcc tggcccaaac gtatcgctgc
      361 aagaatgccc gacatgcccg ccccaatgat caacacgcgg aacgcctgct ttcggtctgc
      421 ttctatgtct tccagcctgt ctccgattct gtcctggcct tcgagcgcca gttcttcgac
      481 gagaaacggt aggtagcggt cgggaatttt cgccccggcg acgaaattca tcgtctcgcg
      541 aatcgtggag acgggagggg aggtggtgcg ggcgaggcgg ccatccctga gtgctccgat
      601 cgccttcact gcggcttcgg cgatttcgga tttttgatgg tcgctcaggc cgccctgccc
      661 atccccgaaa aactcataaa ccggcttgat gccgccccgg ataaggctgg cgtcgcccgt
      721 gagatgaaca agtgccgcgg cgagagaggg atagtgggcg gaggcgatgg ccgcctggat
      781 tgcactgtca ttgtcgcgaa agggaatcgt cattggcgct cccgcctgaa aacccgactg
      841 gcgacatcgg aaatcgacgg cgatactgtc ccccggtcgc cgggagagaa tcaagccgtc
      901 gacaggggcg gttcatgcaa ttcggccggc gcgggcttgg ccgtcgtcaa gccgcttccc
      961 atataggaag cccgccttgg ctccaatcga gaggacatcc cgaatgatcg ctttccttcg
     1021 ctggctgcgg gatattgcac tcggcgccgt gaacggcgtc gtcaggctgg cggtcctcat
     1081 cgtcgttctg gccgccgtcc tgattgtgat cggattgatc gagggcgatg gtatgccgcc
     1141 gcggatggtt ctcaatctcg atcttcggag ttcgcctcca gattcttcga atgcaacgag
     1201 cctgttcttc gatcgacgca ccccgcctgt catggacacg gttttggcgc tcgaccgcgc
     1261 cggccgcgat tcccgggtga agggcgcttt cgtggagctc ggcagtgggg gcctctccgt
     1321 cgcacagggc gaggagatcg gcgccgcgct catccgcttt cgcgccaccg gccggttcgt
     1381 cattgcttac gcccaggggt tcaacagcac cggactgggg gattatctga cggcggccag
     1441 cgccgaccag atctggatgg agccgaggag cccgttcagt gcggcgggct ccggcgccgg
     1501 cgctattttt ctgcggggat tgttcgacaa gattcaggcg gtgccgcaga tcgtcaaacg
     1561 cgccgactac aagagcgcgg ccgatatgta catgcagaag ggctatacgc agcccgatcg
     1621 cgagcagacg accgcatttc tgcaatcctg gtacaacagc gccgtggcgg ccgtctcggc
     1681 cgagcgcaag ctcgacaggc gagcggtcat cgcttctttt caggccagcc cgcaattcgc
     1741 cgaagacgcc cagcgcgcca gattgatcga ccggctcggc tatgacgatc aggcaaagga
     1801 cgcggcccgc gcgcgcgcca accgcgccaa gctcgtcgct atcggcgaat atgcgcgagc
     1861 gaccgagagc gagggcgcgg ttggctcggg gccgcatgtg gctctcatcg aagcggcggg
     1921 cgaaatcgtc gacggcacgg ccgatcacgg cgccctcggc ggcggcacgg tgatcgcggg
     1981 cgatgattat gcggatgcgt ttcgccgggc gacgcgggac cggagcatca aagccattct
     2041 tttccgcatc gattcgccgg gtgggtcggt cactgcgtcg gatcagattc tcgaagcgct
     2101 cagggaagcg aaagccgccg gcaagccggt gatcgtcagc atgggtccgc ttgcggcgtc
     2161 gggaggctac tacgtttcct gtgcggccga ccagatcgtc gcagagccgg cgacgctgac
     2221 gggctccatc ggcgttctta ccggcaaggt cgccatggga aagtcgctcg gaatgatcgg
     2281 cgtcggcgcc gacgagatcg gcgtgggcaa gaacgcactg ttcgactccg gcatcgagcc
     2341 ctata
//
LOCUS       DASZMC010000264         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4246511,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000264 DASZMC010000000
VERSION     DASZMC010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4246511"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VG274_05360"
     CDS             complement(<1..1102)
                     /locus_tag="VG274_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWE06115.1"
                     /translation="MPPRAPLSNKPSSPPIDRALRAPQLLAAGVGALLVLLTVVPLAL
                     LLVGSFRPDGLPLAPGWTFAHYIDVWGSSYDWWVVVNTLVFAGGSTCLAVGIATALSW
                     LFERTDLPGRGLFGAMILMPMATPPLLLAIGWALVLAPRIGIVSVALQPLIGPIDRWF
                     DIYSMPGAIFVQTLAYVPTSVLMLSPAIRALDPALEEAAMVAGASRWQIIWRVGVPLL
                     RPALLSAVTILMIVGMLAFDVPAVIAIPGHVNLMSIEIFRLMTPPSSFPDYGAAAAMN
                     TVLFIFLIAGLVFYRRTIRHAARFATVSGKGYRPARARLGALRGLAVALVFLYFVLAI
                     LLPFVALLWASLIPYFAGFSLHMLQRASFSAYV"
     gene            complement(1123..>1702)
                     /locus_tag="VG274_05365"
     CDS             complement(1123..>1702)
                     /locus_tag="VG274_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HWE06116.1"
                     /translation="GGSVPGETYMGALGNPLAYSLCFPENEERSPWVPLHVQKGFKPT
                     DSTVSVFVGNRGIQEGFGPRDTWEAKFRRCLTATSHIPPCVIVDPIVARLFAARGIKS
                     KDDLINWCVRNARVTARDYWDDQWIQTLVRPRAVAGVEPYATRLKAAPDDIIEMYDAS
                     QINAVVVGGETQGAWKIFSAHYVTTVSVDAWR"
BASE COUNT          384 a          526 c          539 g          253 t
ORIGIN      
        1 ttacataggc ggaaaatgac gcccgctgca gcatgtgcaa gctgaagccg gcaaaatacg
       61 gaatgagact cgcccacaac aaggcaacga acggcagcag gatagcaagc acgaaataaa
      121 gaaaaaccag cgccaccgcc aaaccacgca gcgcgccaag ccgagcgcgc gcgggcctgt
      181 atcccttgcc gctgacggtc gcgaaacggg cagcgtgccg gatcgtccgg cgatagaaca
      241 caaggccggc gatcagaaat ataaacagga ccgtgttcat ggcggcggcc gcgccgtaat
      301 ccggaaagct cgatggcggg gtcatcagtc gaaaaatctc gatgctcatt agattgacgt
      361 gtccgggaat cgcgatcacc gcgggaacat cgaacgccag catgccgacg atcatcagaa
      421 tggtgacggc ggagaggagg gcgggccgca aaagtggcac gcccacgcgc catatgattt
      481 gccagcggct tgcgccggcc accatggcgg cttcttccag cgccggatcc aacgcccgga
      541 tcgccggcga cagcatgagg acggacgtcg gtacataggc cagcgtttgc acaaagatgg
      601 cgccaggcat gctgtagatg tcgaaccagc ggtcgatggg gccgatgagt ggttgcaagg
      661 cgacactgac gatgccgatg cgcggggcga gtaccagagc ccacccgatc gccagcaaca
      721 gcggcggtgt cgccatgggc atgagaatca ttgcgccaaa caggccgcgg cccggaagat
      781 cggtccgctc gaacagccaa ctcaacgcgg tggcgatgcc cacggccaaa caagtgctgc
      841 cgccggcgaa gaccagcgta ttgacgacaa cccaccaatc gtacgacgag ccccaaacat
      901 caatgtaatg cgcaaacgtc cagcccggcg cgagcggaag tccatcgggg cgaaagctgc
      961 cgacaagcaa cagggcgagc ggtaccaccg tgagcagaac gagcagcgcg cctacacccg
     1021 cggcgaggag ctgtggtgcg cgcagcgcac gatcgatcgg aggtgagctt ggcttattcg
     1081 agagaggcgc gcgcggtggc aagaaaatct cagcccatcc actcaacgcc acgcgtcgac
     1141 cgacaccgtc gtcacataat gggcgctgaa gatcttccag gcgccctgcg tttcaccgcc
     1201 gacgaccacg gcgttgattt ggctcgcgtc atacatctcg atgatgtcgt ccggcgcggc
     1261 cttaagccgt gtcgcgtatg gctcgacgcc tgcgaccgcg cgcggccgaa ccagcgtctg
     1321 aatccactga tcgtcccaat agtcgcgcgc cgtgacgcgg gcgttccgca cgcaccaatt
     1381 gatcagatca tccttcgact tgatgccgcg ggcggcgaac aggcgcgcga cgatcggatc
     1441 gacaataacg caaggtggga tgtgcgatgt ggcggtgagg cagcggcgga atttcgcttc
     1501 ccaggtgtcg cgcggcccga agccctcctg gattccgcga ttaccgacga aaacgctgac
     1561 cgtgctgtcc gttggtttga atcccttttg gacgtgcagc ggtacccatg ggctgcgctc
     1621 ctcgttctcg ggaaagcaca ggctgtaggc caggggattg ccgagcgcgc ccatataggt
     1681 ttcgccgggc accgagccgc cc
//
LOCUS       DASZMC010000265         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1237202,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000265 DASZMC010000000
VERSION     DASZMC010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237202"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..484
                     /locus_tag="VG274_05370"
     CDS             290..484
                     /locus_tag="VG274_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06117.1"
                     /translation="MEFSKIATPATSATQQAASLVSIGYGVSSLPLLGMTCAQAIERA
                     TSAATAYYNPSLQLQLPWQH"
     gene            complement(432..644)
                     /locus_tag="VG274_05375"
     CDS             complement(432..644)
                     /locus_tag="VG274_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06118.1"
                     /translation="MSLQSRGRIDIGGRTGFVGKLHEIDIFGMQPAATIGEMIHREIG
                     PGGWERSAAINVATAVEVGATDYSRL"
     gene            579..1730
                     /gene="lysA"
                     /locus_tag="VG274_05380"
                     /pseudo
     CDS             579..1730
                     /gene="lysA"
                     /locus_tag="VG274_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
BASE COUNT          322 a          563 c          525 g          320 t
ORIGIN      
        1 ccgaggccga tcaataaggc ggcggcgaga agagccttgc gcgtcatttg attctgcatc
       61 tccttagaga tgtgcattcc tgatttagcg caaccgttcc aaaaaatgcg cggcgcacga
      121 ctggttgact actgaacagc actcaacgcg aagagcagac tatggcaaag cagagcctcg
      181 gtaaagttca gaaacgagta gtttcaaaca tcgttcttga ctcgattctc attggtgcca
      241 cattctttgt cgccattcgt atggcgccat tacgctgggg gaatatttca tggaattcag
      301 caagatagcg acgcccgcca catccgcgac tcaacaggcc gcaagcctcg tttccatcgg
      361 ctacggcgtc tcttccctgc cgctcctggg catgacttgc gcacaggcga tcgagcgtgc
      421 cacaagcgcg gctacagcct actataatcc gtcgctccaa cttcaactgc cgtggcaaca
      481 ttgatggcgg cggatctctc ccagccgccg ggcccgatct cgcgatgaat catttcgcct
      541 atcgtggcgg ccggctgcat gccgaagatg tcgatctcat gcagcttgcc gacgaagccg
      601 gtacgcccgc ctatgtctat tcgaccgcga ctctggagcg acactaccgc gtcttcgcgc
      661 gcgcctttcc tgcggacgcg ctgatcgcct tttccgtcaa ggcgaacggc aatctcgcgg
      721 tgctgaagac cttggcgcgg ttcggcgcag gcgccgacgt ggtttcgggc ggcgagctgg
      781 agaaagcgct gagggccgga atcgcgcccg ccaggatcgt cttctccggc gtcggcaaga
      841 cgaagcccga gatggcgcgc gctggaggcg ggaattcatc agttcaatgt ggaaagcgag
      901 ccggagctcg aagcgctgga ccaggtcgcg cggagcctcg gacgaaccgc gcccgtcacc
      961 atccgcgtca atcccgacat cgacgcgcgc acccacgcca agatcgccac cggcacggcg
     1021 gagacgaagt tcggcgtacc ttggcgccgc gcccgcagcg cctatcgcct cgccgcctcg
     1081 ctcgcctcgc tcaaggtggt gggcgtcgac gtgcatatcg gcagccagat tgccgagctt
     1141 cagccgttcg aagaagcttt cgcgaagctc gcgcaactca ttgccgatct gcgcgccgac
     1201 gggcacgaga tcgcgcgcgc cgatctgggc ggcggtctcg gcgtgcccta tcacgccagc
     1261 gacaaggcgc cgcccgatcc gcaagcctat ggggcgatga tcgcgcgcgt ttcggagaaa
     1321 ctgggcgtgc gtctcattct cgaaccgggg cggctgattg cggccaatgc cggaattctg
     1381 ctttcccgga tcatctttgt gaagcagggc gaagcgcgcc aattcctgat catcgacgcg
     1441 gcgatgaacg acctcatccg cccggcgctc tacgacgccc atcacgaaat cgtgccggtg
     1501 cggcagccgg atgcgacgga cggcaccgaa cgctacgaca ttgccggtcc ggtctgcgaa
     1561 accgcagatc ttttcgcccg cggccgcgag ctgccgcatc tggaaagcgg cgacctcgtg
     1621 gccatcatga cggcgggcgc ctatggcgcg gtcatgtctt ccgcctacaa tgcccgccct
     1681 cctgcctgcg aggttctggt gaaagacgcg gcgtggagca ttgtgcggcc
//
LOCUS       DASZMC010000266         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2315577,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000266 DASZMC010000000
VERSION     DASZMC010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315577"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VG274_05385"
     CDS             <1..237
                     /locus_tag="VG274_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWE06119.1"
                     /translation="ATLTGVRSVNDPTATNVVQIILSGAHRQISDAHTTMPAFGSAYS
                     DQEIASLANYVTARFGAQPSAVTARTIETLRAQD"
     gene            complement(318..1124)
                     /locus_tag="VG274_05390"
     CDS             complement(318..1124)
                     /locus_tag="VG274_05390"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HWE06120.1"
                     /translation="MNAIKRLPTLFIPHGGGPCFFVEPPASSPHMWDGLAAYLRGIDA
                     SLGVRPKAVLVISGHWENPLPTLNISAKPGLLFDYYGFPDHTYKLKYPAAGAPDLAER
                     VRKLLGDAGFDSDVETKRGLDHGVFVPFLLIYPNADVPILQLSLQASLDPALHLELGR
                     ALAPLRDQGVLIVGSGMSYHNLADLFSGRGAASAAAFDAWLGDAVRDPATRHELLTQW
                     HEAPGGAASHPREEHLIPLMVAAGAAQGERGFRSFGESIGGKQISGFQFG"
     gene            complement(1188..>1523)
                     /locus_tag="VG274_05395"
     CDS             complement(1188..>1523)
                     /locus_tag="VG274_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06121.1"
                     /translation="GTAAAAPSSGNASASTGAGQYQGSLASSLQTLIQQVGSTGATTP
                     AVSNLESSFNTLMQGLGGSTASTASAAGGTGSAQSSTASLQSFLNNLLQNLQTNGLQL
                     SGSSVNVKA"
BASE COUNT          288 a          483 c          490 g          262 t
ORIGIN      
        1 gcgacgctca ccggcgtgcg ctcggtgaac gatcccacgg cgaccaatgt ggtgcagatc
       61 attctgtccg gagcacatcg gcaaatctcg gatgcgcaca ccaccatgcc ggccttcggc
      121 agcgcttatt ccgaccagga aatcgccagt cttgcgaatt acgtcaccgc gcgattcggc
      181 gctcagccct cggcggtcac cgcgcgaacc atcgaaaccc tgcgcgctca ggactagctt
      241 gggtgtctgc gctccgccca tcgcgagtga actcgcatcg ggaccgggcg ccagcgctca
      301 gagctggcgg cctgcgttca gccgaactga aagcccgaga tttgcttgcc gccgatggat
      361 tcgccgaagc tgcgaaatcc gcgctcgcct tgcgcggcac cggcggcaac catcagagga
      421 atgagatgtt cctcgcgcgg atgcgaggcc gcgccgcccg gcgcctcgtg ccactgggtg
      481 agcagctcgt ggcgggtcgc agggtctctc acggcatcgc ccagccaggc atcgaaggcg
      541 gcggccgacg ccgcgccacg gcccgagaat aggtcggcca gattgtgata actcatgccg
      601 ctgcccacga tcagaacacc ctgatcgcgc aacggcgcca aggccctgcc cagttcgagg
      661 tgcaaagccg gatcgagact ggcttgcagc gatagctgca gaatgggcac gtccgcattc
      721 ggatagatga gcagaaaggg cacgaacacc ccgtggtcca ggccccgctt ggtttccacg
      781 tcgctgtcga acccggcatc gcccaggagc ttgcgcaccc gctcggcgag atcaggcgcg
      841 ccggccgccg gatatttcaa cttgtaggtg tgatccggga agccgtagta atcgaacagc
      901 aaacccggct tggcggagat attgagcgtg ggcagcgggt tctcccaatg ccccgaaatc
      961 accagcaccg ccttgggcct gacgcccaga gaggcgtcga tgcctcgcag ataagcggcc
     1021 aagccgtccc acatatgagg cgacgatgcc ggcggttcca cgaaaaagca tgggccgccg
     1081 ccatggggaa tgaacaatgt cggcagtctc ttgatggcgt tcacatggaa ttttacaggc
     1141 ggccccgagc ctagcctggc gacctgcgaa cgtcaattgg cggactgcta ggctttgacg
     1201 ttgacgctcg atccggataa ctgcagtccg ttggtttgca ggttttgcag caaattattc
     1261 aagaagcttt gcagcgatgc cgtcgaggac tgagccgacc ccgtgccgcc ggcggccgag
     1321 gccgtcgatg cggtgctgcc gcccaatccc tgcatcaagg tgttgaacga gctctcgaga
     1381 ttggagacgg cgggagtcgt tgctccggtc gagccgactt gttgaatcag agtctgcagt
     1441 gaagaggcga ggcttccctg gtattggcct gcgcccgtgg acgccgaggc gttgccgctg
     1501 gacggagccg cggcggcggt gcc
//
LOCUS       DASZMC010000267         9124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1498406,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000267 DASZMC010000000
VERSION     DASZMC010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498406"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VG274_05400"
     CDS             <1..626
                     /locus_tag="VG274_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008303469.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWE06122.1"
                     /translation="AVVNEWRAAWDSLDANSRAAKMARQGVRYHTLEALCIMDPETME
                     PVPADGQSLGEVMFRGNIVMKGYLKNAPATREAFAGGWFHSGDLGVLHADGYIQLKDR
                     SKDIIISGGENISSIEVEDALLKHPSVLHAAVVARPDEKWGETPCAFVERKKGHDRTT
                     ERELLAWCRDMLAHYKCPRHIVFCELPKTSTGKIQKFKLRDRAKDVE"
     gene            complement(1001..1327)
                     /locus_tag="VG274_05405"
     CDS             complement(1001..1327)
                     /locus_tag="VG274_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06123.1"
                     /translation="MKTIAKIAVSAAMLAGAALATTAPASAGVSVGVSVGAPGVVVGD
                     GNPCNRPFRFRPAYCGYPVYGRPLYVDGVSYRGPVYVRTVGAERFFWMHNRWERDRRE
                     WHRDVR"
     gene            complement(1608..1763)
                     /locus_tag="VG274_05410"
     CDS             complement(1608..1763)
                     /locus_tag="VG274_05410"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HWE06124.1"
                     /translation="MQELSEKHKKLQELIESEMAHPDWDEVRVAALKKEKLRVKDEIE
                     RLRSCVH"
     gene            1965..2171
                     /locus_tag="VG274_05415"
     CDS             1965..2171
                     /locus_tag="VG274_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HWE06125.1"
                     /translation="MTEAEEQSLRAALAALIEEHRDLDSAILALVSTGSGDQLQLTRL
                     KKRKLQLKDQIARLENELLPNIIA"
     gene            complement(2172..2879)
                     /locus_tag="VG274_05420"
     CDS             complement(2172..2879)
                     /locus_tag="VG274_05420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWE06126.1"
                     /translation="MKIARLLRSVTSTTPDPPPPGSSGTRRESPDPSTASSFLGISSS
                     ELTPGVRRVVTALLGEARALHHELGRTRALLEEAEEVADRDHLLPILNRRAFIRALDR
                     EIASIARYATPSTLVYFDLDDFKRINDANGHACGDAVLAHFVSLLLSHIRGSDVAGRL
                     GGDEFGLILIHAKREQARRKSASLAGLLDSAPASFAGNPLPFSFSWGVLELTGAMSAE
                     AAIALADAEMYRRKRER"
     gene            2975..3562
                     /gene="purE"
                     /locus_tag="VG274_05425"
     CDS             2975..3562
                     /gene="purE"
                     /locus_tag="VG274_05425"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWE06127.1"
                     /translation="MVPFLAGRGPYNAALLGFGAACPEQTGSMTVSSPLVGLIMGSQS
                     DWPTLRAAAEVLDALAIPHEARIISAHRTPDRLYAYAKKAAERGLRVIIAAAGGAAHL
                     PGMTASMTCLPVLGVPIETSALKGMDSLLSIVQMPGGVPVATFAIGEGGARNAALHAA
                     AILALSDDALASRLQAWRTRQTEAVAEHPVEPSGR"
     gene            3559..4674
                     /locus_tag="VG274_05430"
     CDS             3559..4674
                     /locus_tag="VG274_05430"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWE06128.1"
                     /translation="MTGSAQTPLPVGPGGMIGILGGGQLGRMLSLAAARLGLRTHVYS
                     DEADAVALDVATLRTIASFDDKAALTEFARSCDAITFEFENVPSAAVAFLAQEKPTHP
                     DARALAITQDRFAEKTFLSDLGIGTAEFERADTFAEASAALSRTGAPAILKTRRLGYD
                     GKGQAMIATDGDLASALQSLRSAPAILERLVDFAFECSVVAARAIDGSFAPYDPPENL
                     HGDHILRRSRVPARLTPAQAAQAQATAKQIADALDYVGVLAVEFFLTRSSALLVNEIA
                     PRVHNSGHWTIEACVVSQFEQHIRAVAGWPLGDPSRHSDAVMDNVIGAEAENWRSLAR
                     GTGALHLYGKRDIRPGRKMGHVTRLSPLGAAKPIPPD"
     gene            complement(4625..5332)
                     /locus_tag="VG274_05435"
     CDS             complement(4625..5332)
                     /locus_tag="VG274_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HWE06129.1"
                     /translation="MTAKAEKAREPRTRRGAKKDPKLAVLSAALAHVPFDGFTDKVLE
                     QAAREAGVPADTLARLFPEGPLSLVEAFSDQVDAEMTQKLSKVKLSNLKVRERIATAV
                     KLRLVSLRPHKEAARRAAAFLTLPPHATTGMKLLYRTVDAIWRGIGDTSTDFNFYTKR
                     AILAAVYSTTLMRWFSDTGEDEHVTDEFLRHRIDDVMRFEKFKSQLRERASGWPSISE
                     FLNPAGSASRRPGARGA"
     gene            complement(5329..5883)
                     /gene="def"
                     /locus_tag="VG274_05440"
     CDS             complement(5329..5883)
                     /gene="def"
                     /locus_tag="VG274_05440"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWE06130.1"
                     /translation="MSILKIARMGHPVLAQIAEPVADPAAPQIRRLVADMVQTMIDAN
                     GAGLAAPQVHVPLRVLVFQAPKERSQGQLSEEERFDHTAPVTVLINPQIEILGSETEG
                     GWEGCLSIPGLRGFVERPAHIRYRGLDGTGREIVRTAKGFHARVVQHECDHLDGILYP
                     QRMSDLKSLIFETESRHWAPREAI"
     gene            6018..6230
                     /gene="rpsU"
                     /locus_tag="VG274_05445"
     CDS             6018..6230
                     /gene="rpsU"
                     /locus_tag="VG274_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWE06131.1"
                     /translation="MQIIVRDNNIDQALKALKKKMQREGIFREMKLRGHYEKPSEKRA
                     RERAEAIRRYRKLQRKRLQREGVLPR"
     gene            complement(6384..7301)
                     /locus_tag="VG274_05450"
     CDS             complement(6384..7301)
                     /locus_tag="VG274_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWE06132.1"
                     /translation="MTDTHEHGLWTHDHHFLGHGHERAERRARVAASITAVFMAVEIT
                     AGIVFRSMALLADGVHMATHVGALGLAAGAYWLARRHTANARFTFGSGKFGDLAAFAS
                     AIVLGVIAAGVAVESVARLLFPVVVDYRNALIIAAVGLAVNITCAIVLSDEHDHGHAR
                     DHNIRAAYIHVLADAATSVLALGALAGGLFFGVRWLDAAVGCIGAGVIASWAFGLVRD
                     SGLVLLDAEADPDVARQIRSMIEGELGARVADLHLWRLGPGHHGLIVSLVSPGPTTAE
                     TVKAALRDRHPDLSHVTVEVVVCADCATG"
     gene            complement(7367..8761)
                     /locus_tag="VG274_05455"
     CDS             complement(7367..8761)
                     /locus_tag="VG274_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HWE06133.1"
                     /translation="MKADIAAVAYLVSGVLFILALRGLSSPETSRTGNARGMAGMALA
                     IVTTLWVAGVSDKLTWGLIAGGIAVGGTIGAVVARRIAMTQMPQLVAAFHSLVGLAAV
                     LVAAAAMYSPQAYGIGTEGAIRMQSLIEMSLGVAIGAITFAGSLIAFAKLNGNMSGAP
                     ILLPARHALNLLIAAGIVALIIWFVMAQPHWSFWAVVGLAFVFGITLIIPIGGADMPV
                     VVSMLNSYSGWAAAGIGFTLENTALIITGALVGSSGAILSYIMCKGMNRSFVSVIMGG
                     FGGEVAAAAAAETRSVKQGAAEDAAFIMKNAGSVIIVPGYGMAVAQAQHAVREMADIL
                     KKEGVKVSYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSQFATTDVAYVIGAN
                     DVTNPAARNNPQSPIFGMPILDVEKAKTVLFVKRGMGSGYAGIENELFFGDNTMMLFG
                     DAKKMTEGIVKALG"
     gene            complement(8758..9066)
                     /locus_tag="VG274_05460"
     CDS             complement(8758..9066)
                     /locus_tag="VG274_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004664154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HWE06134.1"
                     /translation="MEAIDPFVFRLSIFVLAIFVGYYVVWSVTPALHTPLMSVTNAIS
                     SVIVVGALIAVSVAAFGGTSWASKILGFFALVMACVNIFGGFLVTGRMLAMYKKKEKR
                     "
BASE COUNT         1802 a         2908 c         2800 g         1614 t
ORIGIN      
        1 cggcagtcgt caatgaatgg cgggccgcgt gggacagtct cgacgcgaat tcgcgcgcgg
       61 cgaaaatggc ccgccagggc gtgcgctatc acacgctcga ggcgctttgc atcatggatc
      121 ccgagaccat ggaacccgtc cccgccgacg gccagtcgct cggagaggtg atgtttcgcg
      181 gcaatatcgt catgaagggt tacctgaaaa acgcgccggc gacgcgcgaa gcgttcgccg
      241 gcggctggtt ccattccggc gatctcggcg tcctccatgc cgacggctac attcagctca
      301 aggaccgctc gaaggacatc atcatttcgg gcggcgagaa catctcgtcg atcgaagtgg
      361 aggacgcgct tctgaagcat ccctcggtgc tgcacgccgc agtcgtggcc cgccccgacg
      421 aaaaatgggg cgaaacgccc tgcgcctttg tggaaaggaa aaagggacac gacaggacga
      481 ccgaacgcga actcctggcc tggtgccgtg acatgctcgc gcactacaaa tgccctcgcc
      541 acattgtatt ctgcgaacta cccaagacat cgacgggcaa gatccagaaa ttcaagctgc
      601 gcgaccgcgc aaaagatgtg gaataatccg gcggcgtctc ttttctctgc gatttcagga
      661 attcacgaca gacaacgagc aggaagactc agcgcagagg acgcagagga tgtgcagggg
      721 agacggggca aaagaattca caatgagaca gtgaggaagt aggatcgcgc gccgacccgc
      781 ctacgccatg gcttcggagc agcgtaggag gaaggcgcgc aacaagtctc tcgctgtcct
      841 cactgtctca ttgtgaatct tccccttctc tgcgtcccct gcgcatcctc tgcgtccact
      901 gcgttaaatt ttctcccttc gagctctttg tgcctttgtg atgaggaacg ccggtcccgg
      961 cgccgcggta cgggccggga ccggacgaaa cgcagagcgc ttagcgaacg tcgcggtgcc
     1021 attccctgcg gtcgcgttcc cagcggttgt gcatccagaa gaaccgctca gccccgactg
     1081 tgcgcacata gacgggcccg cggtacgaga cgccgtccac gtagagcgga cggccataga
     1141 ccggataacc gcaataggcc ggccgaaagc ggaagggtcg gttgcaggga tttccgtcgc
     1201 cgacgactac gcccggcgcg ccaaccgaca cgccgacgct gacacccgcg cttgccggag
     1261 ccgtcgtggc cagggccgcg ccagcgagca tggctgcgct cactgcaatc ttcgcgatcg
     1321 ttttcatctt cagtctcctt tgcgccggcg cctgaatggc gaaccgacat gcgcgcaaac
     1381 atggttccat tgctttgaac tcgcgctgaa gttgcggttc agacggcatt catcgctgca
     1441 agaaggattg ccgacggacg tggatggcga cactccgtta caggaatcgc cacgcctcag
     1501 gaccggggct caaagaagat ccgctttgcg cgctccgctg cgattcgtcg aggcacgcaa
     1561 ccggacgccg gcaaaggcga ccggccggaa cgcggcgctt gagcgcgtca gtggacgcag
     1621 ctgcgcaggc gctcgatttc gtcttttact ctcaactttt cttttttgag ggcggcgacc
     1681 cggacttcgt cccaatcggg gtgggccatc tcggattcga tcagttcctg gagttttttg
     1741 tgcttttcgg acagttcctg catgtgtcca tctagtgcca ttgttcgcac ctcattcaca
     1801 aaagaggcac aattaaatca ccatcggcgc ttcctgtcca cgatccgaga ctggcttggc
     1861 ttccggcccg caaacggccg gcgcgtttcc cgagtggggg acaatctccc cgatgaaacc
     1921 ttgcggaagg gccaacgcgg gccaactggg gtatgatccc tcggatgacc gaagcagaag
     1981 aacagagcct gcgcgctgcg ctggcggcgc tgatcgagga acaccgcgac ctcgattcgg
     2041 ccatcctggc gttggtctcg acagggtcgg gcgaccagct tcagcttacc cggctcaaga
     2101 agcgcaaact gcagctgaag gaccagattg cccgcctgga aaacgagctc ttgccgaaca
     2161 tcatcgcctg atcatcgctc gcgcttgcgc cggtacattt cagcgtccgc cagcgcgatc
     2221 gccgcctctg cgctcattgc gccggtcagt tccagaacac cccaggagaa ggaaaaggga
     2281 agcggattcc ccgcgaaact cgccggcgcc gagtcaagaa ggccggcgag gctcgccgac
     2341 tttctccgcg cctgttcccg cttcgcatga atcagaatga ggccgaattc atcgccgccg
     2401 agccggcccg cgacgtcgct cccacggata tgagaaagca gaagcgagac aaaatgggcg
     2461 agcaccgcat cgccacaggc gtgcccgttg gcgtcgttga tcctcttgaa atcgtcgaga
     2521 tcgaaataga cgagagtcga aggcgttgcg tagcgcgcga ttgacgcgat ttcccgatcg
     2581 agtgcacgta tgaaggcacg gcgattgagg atcggcagga gatggtcgcg atcggccact
     2641 tcttcggctt cctcgagaag tgcgcgggtg cggccaagct cgtggtgaag cgcacgcgct
     2701 tcacccaaaa gggcagttac cacgcggcgc acgccgggcg tgagctcgct gctggaaatg
     2761 ccgaggaagc tcgacgcggt ggacggatca ggggattcgc gccgcgtgcc gctggagcct
     2821 ggtggaggtg ggtctggggt tgtcgaagtt accgaacgaa ggagccgcgc gatcttcatg
     2881 acgtccgccc ttcgtcgatt cgatccgttc aagacgatcc atgccaacag gttaacgaaa
     2941 catttatcct gtcccgcgca acggcgcacg gcagatggta cctttccttg cggggcgcgg
     3001 cccgtataat gcggcgcttc tcggcttcgg ggcggcgtgc cccgaacaga caggaagcat
     3061 gaccgtctcc tccccgcttg tcggcctcat catgggcagc cagtccgact ggccaaccct
     3121 gcgcgcggca gcggaggttc tcgacgctct ggcaatcccc cacgaagccc gaatcatttc
     3181 ggcccaccgc acgccggacc gcctctacgc ctatgccaaa aaggcggcag aacgcgggtt
     3241 gcgggtgatc attgcggcgg caggcggcgc cgcccacctg cccggcatga cggcctccat
     3301 gacctgcctt cccgtgctcg gcgtgccgat cgaaacctcg gcgctcaagg gcatggacag
     3361 tctcctgtcc attgtacaaa tgcccggcgg cgtgccggtc gcaactttcg cgatcggcga
     3421 gggcggcgca cgcaacgcag cccttcatgc ggcggcgatt ctcgcgcttt ccgacgacgc
     3481 gcttgcctct cgtcttcagg catggcgcac ccgccagact gaggcggttg ccgaacatcc
     3541 ggtagaaccc tccggacgat gacaggttca gcgcagactc ctctccccgt cggtcccggg
     3601 ggcatgatcg gcattctggg tggcgggcag ttgggacgga tgctttcgct tgctgcggcc
     3661 agactcggtc tgaggacaca cgtctactcg gacgaggcag acgcggtggc gctcgatgtc
     3721 gccacccttc gcaccatcgc gtctttcgac gacaaagccg ccctcacgga attcgcgcga
     3781 tcttgcgatg ccatcacctt cgaattcgag aatgtgccaa gtgccgctgt cgcgtttctg
     3841 gcgcaggaga agccaaccca tcctgacgcg cgcgccctcg ccataaccca ggaccggttc
     3901 gcggaaaaaa ccttcctgtc cgatctcgga attggaactg cggaatttga gcgcgccgac
     3961 acgtttgccg aggcgagcgc ggctttgtcg cgcaccggtg cgcctgccat cctcaagacg
     4021 cggcggctcg gttatgacgg caaaggtcag gcaatgatcg caaccgacgg cgatcttgcc
     4081 tccgccctgc agtcattgcg gtcagcaccc gcgatactgg aaaggctggt ggacttcgct
     4141 ttcgagtgct cggttgttgc tgcgcgcgcg atcgatggtt cgtttgcccc ctatgacccg
     4201 ccggagaatc tgcacggcga tcacatcctg cgtcgttcgc gggttcccgc acggcttacg
     4261 cctgcgcaag cagcgcaggc ccaggcaacc gcaaagcaga ttgccgatgc tctcgactat
     4321 gtcggtgtac tggcggtcga gttcttcctg accagaagtt cggcactgct cgtcaacgag
     4381 atcgcgccgc gcgtgcacaa ttccggccac tggacgatcg aggcatgcgt cgtcagccag
     4441 ttcgaacagc acatccgcgc cgtcgctggc tggcctctcg gcgatccctc gcgacattcc
     4501 gacgccgtga tggacaacgt catcggggcg gaagcggaaa actggcggtc tctcgcccgc
     4561 gggaccgggg cgcttcatct ctacggcaaa cgggacatcc gtcccggccg caagatgggg
     4621 cacgttacgc gcctctcgcc cctgggcgcc gcgaagccga tcccgccgga ttgagaaact
     4681 ccgagatcga cggccagccg ctcgcccgct cgcgcaactg cgacttgaac ttttcgaaac
     4741 gcatgacgtc atcgatgcgg tgccgcagga actcgtcggt gacgtgttcg tcctcgcccg
     4801 tatcggagaa ccagcgcatc agcgttgtgg aatagacggc agcgagaatc gcgcgtttgg
     4861 tgtagaagtt gaaatcggtc gaagtatcgc cgataccgcg ccagatcgcg tcgacagtgc
     4921 ggtagagaag cttcatgccc gtcgtcgcgt gcggcggaag cgtgagaaac gccgcggctc
     4981 ggcgcgccgc ctccttgtgc ggccggagag acaccagacg aagcttgacg gccgtcgcga
     5041 tccgctcgcg caccttgaga ttcgagagct ttaccttcga aagcttttga gtcatctccg
     5101 catccacctg atcggagaat gcttcgacga gactgagagg accttcggga aaaaggcggg
     5161 cgagcgtgtc agcgggaacc cccgcttcac gcgccgcctg ttcaaggact ttatcggtga
     5221 agccgtcgaa gggaacatga gcgagtgcgg ccgaaaggac ggcgagcttc ggatccttct
     5281 tcgcgccgcg tcgggtgcgg ggctcgcgcg ctttctctgc tttggctgtc atatcgcctc
     5341 cctgggcgcc caatgacgcg attcggtttc gaaaatgaga cttttcaggt ccgacattcg
     5401 ctgcggatag agaatgccgt ccagatgatc gcattcgtgc tgcaccacgc gggcatgaaa
     5461 gcctttcgcg gttcgcacaa tttcccgccc cgttccgtcg agcccgcgat agcggatatg
     5521 cgcgggtcgc tcgacgaatc ccctcaggcc tggaattgac aggcagcctt cccagccgcc
     5581 ctccgtttcg gagcccagga tctcgatctg cggattgatc aataccgtca ctggcgcggt
     5641 gtgatcgaat ctttcctcct cggacaattg accttgcgaa cgctccttgg gcgcctggaa
     5701 aacgaggacg cgcagcggca catgaacctg cggcgccgca agcccggccc cgttggcgtc
     5761 gatcatggtc tgaaccatgt cggcgaccag gcggcgaatc tgcggcgccg cgggatccgc
     5821 aacgggctcc gctatttgcg ccagaacggg atggcccatg cgggcaatct tgaggatgga
     5881 catggcctga atatgggatt gacgccgccg cccgtcaaag ccgccccttc cagggtattc
     5941 ttcccgggat agacagttgt acgcccgtct gatatctagc gccgccctta acgaaaccag
     6001 ggaaaccgga gccgcctttg cagatcatcg ttcgcgacaa caatatcgac caggcgctga
     6061 aggcgctgaa gaagaagatg cagcgggaag gcatcttccg cgagatgaaa ctccggggcc
     6121 attacgaaaa accttccgaa aaacgtgccc gtgagcgcgc ggaagcgatc cgccgctatc
     6181 gcaagcttca gagaaagcgc ttacagcgcg agggcgtcct tccccgctag gttttgaaaa
     6241 agggcgcatg ctcagcccta ccggcgtttc ccgcgaggaa atcgagagga gcggccgcac
     6301 gctcctgcgc cgcgcaaacc catgaagcgc cgccacgggc tgagaaccgc caggcaggcg
     6361 ctcgatttcg tggcgaagga aggttagccg gtcgcgcagt cggcgcagac caccacctcg
     6421 accgtcacat ggctgagatc gggatggcgg tcgcgcaggg cggccttgac cgtttcggct
     6481 gtcgtcgggc cgggactcac cagtgagaca atcagcccat gatgccctgg gccaagccgc
     6541 cacagatgga gatcggcgac acgggcgccc aactcgcctt cgatcatcga cctgatttgt
     6601 cgcgcaacat ccggatccgc ctccgcgtcg agcagcacca gaccgctgtc gcgcaccaga
     6661 ccaaaagccc atgacgcaat cacgcccgcg ccgatgcagc cgaccgccgc atccagccag
     6721 cgcacgccga agaaaaggcc gccggccagc gccccaaggg cgagcaccga ggttgcggcg
     6781 tcggcaagga catggatata ggctgcgcgg atgttgtgat cgcgcgcatg tccgtgatcg
     6841 tgttcgtcgg acaggacgat tgcgcaagtg atgttcaccg ccaatccgac cgcggcaatg
     6901 atcagcgcgt tgcggtagtc gacgacgacg gggaataaca gtcgcgcgac ggattcaacg
     6961 gctactcccg ctgcaatcac gcccagaacg attgcgctgg cgaacgccgc caggtcgccg
     7021 aactttcccg agccaaatgt gaaccgcgca ttcgccgtgt gccgcctcgc cagccagtag
     7081 gcgccggccg ccagtcccaa tgcgcccaca tgcgtcgcca tgtgcacgcc gtccgccagc
     7141 agcgccatcg agcggaaaac aattcccgcc gtgatctcca ccgccatgaa aacggcggta
     7201 atcgacgccg caactcgcgc gcgcctctcc gcgcgttcgt gcccgtggcc gagaaaatga
     7261 tggtcgtgcg tccacaggcc gtgttcatgg gtatcggtca tggcggcaga atagtatgac
     7321 ggcgcgcgcg aaaccaatgg aaagacggtt tgtgcagccg ttccattcac ccgagtgctt
     7381 tcacgatccc ttcggtcatc ttcttcgcat cgccgaagag catcatggtg ttgtcgccga
     7441 agaacagctc gttctcgatt ccggcatagc ccgagcccat gccgcgcttg acgaacagca
     7501 ccgtcttcgc cttttcgacg tcgagaatgg gcatgccgaa gatcggcgat tgcggattgt
     7561 tcctggcggc gggattggtg acgtcgttgg ccccgatgac ataggccaca tcggtggtgg
     7621 cgaactggct gttgatgtct tcgagttcga agacttcgtc atagggcacg ttcgcttcgg
     7681 cgagcagtac gttcatgtga ccgggcatgc ggccggcgac ggggtgaatg gcgtaggaga
     7741 ctttcacccc ttccttcttg agaatgtccg ccatttcgcg cacggcatgc tgcgcctggg
     7801 cgaccgccat gccgtagccc ggcacgatga tcaccgagcc agcattcttc atgatgaacg
     7861 ccgcgtcttc ggccgcaccc tgctttacgg atcgcgtctc cgccgcagcg gccgcggcca
     7921 cttcgccgcc aaacccgccc atgatcaccg aaacgaagga gcgattcatc cccttgcaca
     7981 tgatgtagga gaggatcgcg cccgacgaac cgacaagcgc gcccgtgatg atgagcgcgg
     8041 tgttctcgag ggtgaagccg atgccggcgg cggcccaacc ggaataggag ttcagcatcg
     8101 acacgaccac gggcatgtcg gcgccgccga tcggaatgat gagcgtgatg ccgaagacaa
     8161 aggcgagccc caccacagcc cagaaactcc aatgtggctg ggccatcacg aaccagatga
     8221 tgagcgccac gatgcccgcg gcaatcagca ggttgagcgc atggcgcgcc ggaagcagaa
     8281 tgggcgcgcc gctcatattg ccgttgagct tcgcaaaggc gatgagcgag ccggcgaagg
     8341 tgattgcgcc gatggcgacg ccgaggctca tctcgatgag gctctgcatg cggatcgcgc
     8401 cttccgtgcc tatgccatag gcttgcgggc tgtacatggc agcggccgcc accagcactg
     8461 cggcgagacc gacaagactg tgaaatgccg cgacgagctg aggcatctgg gtcatggcga
     8521 tgcgccgcgc gaccaccgct ccaattgtgc cgccgaccgc gatgccgcct gcgataagtc
     8581 cccaggtaag cttatcgctg acgcccgcga cccagagcgt ggtgacgatg gcgagcgcca
     8641 tgcccgccat cccgcgcgca ttccccgtgc gcgaagtctc cgggctcgac agcccgcgca
     8701 aggccagaat gaagagaacg cccgaaacca gataggcgac ggctgcgata tccgccttca
     8761 tcgcttctct ttcttcttgt acatggcgag catgcgcccg gtgaccagaa agccgccgaa
     8821 tatgttcacg caggccatga cgagcgcgaa gaagcccaga atcttcgacg cccaggacgt
     8881 gccgccgaac gccgcaaccg aaacggcgat gagcgcgccc acaacgatca ccgaagagat
     8941 tgcgttggtg acgctcatga gcggggtgtg cagcgcgggc gtcacgctcc agacgacgta
     9001 atagcccacg aagatcgcca gcacgaaaat cgaaagccga aaaacaaagg gatcaatcgc
     9061 ttccatgagg ctcacccaat tctttcctcc cccatcgtgg gggaggtggt ctgcgcagcg
     9121 aagc
//
LOCUS       DASZMC010000268         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_680563,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000268 DASZMC010000000
VERSION     DASZMC010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680563"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VG274_05465"
     CDS             complement(<1..233)
                     /locus_tag="VG274_05465"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 family protein"
                     /protein_id="HWE06135.1"
                     /translation="MSFSIYDASIPPTLRMLDSLSKILDKAVKHAEESKTPLSDLLDA
                     RFASDMFPLTRQVQVVSDAAKGLAARLAGSDPP"
     gene            503..1372
                     /locus_tag="VG274_05470"
     CDS             503..1372
                     /locus_tag="VG274_05470"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWE06136.1"
                     /translation="MNLHNAPLWKASVVVAKQRAGDVAALFEITPPTPQAVLIDEEPF
                     GRNATVEALYAQMPDGDLLSRLAELSIHVGLVPDQDWIRLSQEGLPPVRAGRFFVHGA
                     HDKGMVPNGVIALRIEAGLAFGTGHHESTALCLALLSDTARRRRFGRALDLGCGTGVL
                     AIGLARLWKRRVVAADIDPIAIAVARENASLNSVGPLVGTHVADGVPNATRGRRFDLI
                     AANILAGPLTRLAPQIARAVGPRGVLILSGLLRWQENLVLGFYRPHGFVLRERRRDGN
                     WSALLLERPQRAG"
     gene            1382..>2152
                     /locus_tag="VG274_05475"
     CDS             1382..>2152
                     /locus_tag="VG274_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HWE06137.1"
                     /translation="MDKISSFQSFDDQSDAATCAPRLAALRAELARRGLDGFLVPRAD
                     EHQGEYVPKRAERLAWLTAFTGSAGAAVVLKDKAAIFVDGRYTLQVRAQTDTRLFEPR
                     DLVAEGPAVWLGSNAPEGARLGYDPWLHTASGVEGLRVAAAHAGAELAACESNPIDAV
                     WSDQPEPPLVRAVPHALECAGESAEAKRMRIAEDLKKRGADAGVITLPDSICWLLNIR
                     GGDIPHTPFVLSFAILNADGSTDLFMDERKSSAELTQHL"
BASE COUNT          348 a          687 c          744 g          373 t
ORIGIN      
        1 ctcggcgggt cgctgccggc aagccgcgcc gctaggccct tggccgcgtc gctgacgacc
       61 tggacctggc gggtgagcgg aaacatgtcg gaggcgaagc gggcgtcgag cagatcggaa
      121 agcggggttt tgctctcttc ggcgtgcttg accgccttgt cgagaatctt cgaaagactg
      181 tcgagcatgc gcaatgtagg cgggatggac gcgtcataga tggagaacga catcggtttc
      241 accggttcga gtttgcgagg gcgcacagga aggccaaacg cgaccgggat caaggggccg
      301 cgcgcgcaac ccccatctgc gcgtggggag gagtcgaacg gtctaccccg gcgtgtcatt
      361 cccgcgaaag cgggaagcca gcgcgtaaag cgccgatgcg gttctctctg ggtccccgct
      421 ttcgcgggga tgacacgctt atattgcggg gacatgatat cgctcgtgtg acggtgtgtg
      481 ttactcaatt gagggaaatc gcgtgaactt gcacaatgca cccctgtgga aggcgagcgt
      541 cgtggtggcg aagcagcggg cgggcgatgt ggccgcgctg ttcgagatta cgccacccac
      601 gccgcaggcg gtgctgatcg acgaggagcc gttcggccgc aacgcgacgg ttgaagcgct
      661 ctatgcgcag atgcccgacg gcgatctgct ctcgcgactt gcggaattgt caatccatgt
      721 gggactcgtt cccgaccagg actggatccg gctttcgcag gaaggccttc cgcccgttcg
      781 cgcgggacgc ttcttcgtcc atggcgcaca tgacaaaggc atggtgccga acggagtcat
      841 tgcgctgcgc atcgaggcgg ggctcgcctt cggcaccggg caccatgaat cgacggcgtt
      901 gtgccttgca ctgctgagcg acacggccag gcgccggcgc ttcggccgcg cgctcgatct
      961 gggctgcggc accggcgtgc tcgccatcgg cttggcgcgg ctttggaagc ggcgcgtcgt
     1021 ggcggcggac atcgatccga tagccatcgc cgttgcgcgc gagaacgcgt cgctcaactc
     1081 cgtgggcccg ctggtgggca cgcatgttgc ggacggcgtg ccgaacgcaa ctcgtgggcg
     1141 caggttcgat ctcatcgccg ccaatatcct ggccggtccg ctgacgcggc ttgcgccgca
     1201 gatcgcccgc gcggttggcc cacgcggcgt gctgattctc tcggggctcc tgcgctggca
     1261 ggagaatctc gtgctcgggt tttatcggcc gcacgggttc gtgctgcgcg agcgccggcg
     1321 cgacggaaac tggtcggcgc tgctgcttga gaggccgcag cgggcgggct aacgtcgcgg
     1381 catggacaag atctcctcct tccaaagttt cgatgaccag tccgacgccg caacctgcgc
     1441 gccgagactg gccgcccttc gtgccgaact cgccagacgc gggctcgacg gatttctcgt
     1501 accacgcgcc gacgagcatc agggcgagta cgtacccaag cgcgccgagc ggcttgcgtg
     1561 gctgaccgcc ttcaccggct ccgccggcgc ggcggtggtg ctgaaggaca aggccgcgat
     1621 cttcgtcgat ggccgctaca cgctgcaggt ccgcgcgcag accgatacac ggctgttcga
     1681 accgcgcgat ttggtcgcgg aggggcctgc cgtctggctc ggttcgaatg cgcctgaagg
     1741 ggcgcgcctc gggtacgacc catggctgca cacggcaagc ggcgtggaag gcctgcgcgt
     1801 ggcggctgcg catgcgggcg ccgaacttgc cgcctgtgaa tccaatccga tcgatgcagt
     1861 gtggagcgac cagccggaac cgccgctcgt cagggccgtg ccgcacgcgc tcgaatgcgc
     1921 gggcgaatct gccgaagcca agcgcatgcg catcgccgag gatctgaaga aacggggcgc
     1981 cgatgcgggc gtcatcacgc tccccgattc catctgctgg ctgctcaaca tacgcggcgg
     2041 cgacatcccg cacacgcctt tcgtgctctc cttcgccatc ctcaatgccg acggttcgac
     2101 cgacctgttc atggacgagc gcaagagctc ggcggaactg acccagcatc tc
//
LOCUS       DASZMC010000269         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3670327,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000269 DASZMC010000000
VERSION     DASZMC010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3670327"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            742..1290
                     /locus_tag="VG274_05480"
     CDS             742..1290
                     /locus_tag="VG274_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWE06138.1"
                     /translation="MKARKRTVDVESTTDSETAQIDYGFLWQRVGYVLLKIRAQSFRV
                     FSDLFVGEGLAPGQFHILYLIARNPGLKQIAIGNAAGIDRSTMAPIVDRFEKMGWVQR
                     TRRPDNRRAYSLELTRAGRDLLQKAMPLVDRHERELIGSLTKPEQREVLHLLTKMYQG
                     IIVQDQDSTKPQAVRPDRPSWV"
     gene            1565..2074
                     /locus_tag="VG274_05485"
     CDS             1565..2074
                     /locus_tag="VG274_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWE06139.1"
                     /translation="MSAYIANETAIKRFLGRFLKNVQDIDDTAQEVFMRAFVADAIEP
                     VVTPRPYLFRVAKNLALNHLAKLSNLLTDRIEDVSDTSALPTDHQVSIEDEVDAGQRF
                     GNVVRAIGALPPRCAKVFVLRKIHGFSQKEIALQLCISESTVEKHIALGLVRCGEYLR
                     QQGGALNWE"
     gene            complement(2106..>2932)
                     /locus_tag="VG274_05490"
     CDS             complement(2106..>2932)
                     /locus_tag="VG274_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HWE06140.1"
                     /translation="QRWAIKAGRSDTGTIGCKIGTYWPHNLERGLEAHASTVLLLDPQ
                     TGHPLAVIRASYLTALRTAAADAIGVRHLARPDASRLALVGSGHQAWFDLQAIGLVRA
                     LQDIRVWNRNPERAREFAARACAAGYPARPLALQNALDGADIVVTATAAIGPLFAPEW
                     VSPGAHISAMGADAPGKQELPTELLARARVFVDVIAQSVTIGECQSAYRAGLLDCARL
                     TTLGAVIRGEAPGRSSPDELTVFDSSGTAIQDLSVCARAVAGLQASRNQRELERFD"
BASE COUNT          584 a          862 c          954 g          532 t
ORIGIN      
        1 ggaagggtcg ttcaggcgca tgcgaaccgc gcttcaaggg gggatgaact cgcgaggcgg
       61 ctggtacctg tagacggggc tggcggaatc tgttggcgcc gggtagcttc gagtcgacaa
      121 cctgccaacc aacggaccga caggactgag gagctggagc ctactggacc ggagcgtgat
      181 gaatcaagca gctagcgcag gctccagccg cgaaaatcct attgggaaca ccccgatgcg
      241 gttgaatggg aaacgttttg ggaaaaacga atgagggtgg catctgcagc gatcagtgcg
      301 tctcttaagg cgacctgccg gccgagacgc agtatgtact ctggatcccg gccgattcgg
      361 tctcccttct ccctcgcgag cataagcgat ctatacgtcg atggaagcgt atcgccaatg
      421 gcggcggcgt attccgcaat cgtgtcatat ctgccgccgg ggcccaggcg gcttgtgcgt
      481 gctcgagcgc aagggtcggc aatgacggag ttgattgccg cagcgcgctg atcgtatgcg
      541 tcgacgctcc cgcgatgcgc gatcggacct ggcctttccg gcgctctcat cgacagaggc
      601 atatcgcttt gtgaggcgcg gcgaggccgg cgggaaagcg ctctgacgtg aaggtcgcgg
      661 gcgtccgtgc taggattgag acagttggcg atgggatctg tttgcatcac agccgaaaga
      721 gaaagcgcga gggctccaca ggtgaaggcg agaaagagaa ccgtggacgt ggagtcgacc
      781 acggacagcg agactgctca gatcgactac gggtttcttt ggcaacgggt gggctatgtc
      841 ctgctcaaga tccgcgcgca gtcattccgt gtcttcagcg atctgttcgt tggggagggg
      901 ctcgcgccgg gacagttcca catcctgtat ctgattgccc gcaatcccgg cctgaagcag
      961 atagccatcg ggaatgcagc tgggatagat cgctccacga tggcccctat cgtcgaccgg
     1021 ttcgagaaga tggggtgggt gcaacgcacg cgtcgtccgg acaaccggcg ggcgtactct
     1081 ctcgaattga cccgggcggg cagggatctc ttgcagaaag cgatgccgtt ggtcgaccgc
     1141 cacgagcggg agttgatcgg ctccctgacg aagcccgagc agcgcgaggt gctgcatctc
     1201 ctgacaaaga tgtaccaggg gatcatcgtg caagaccagg acagcacgaa gccgcaggcg
     1261 gtacgtcccg atcgaccgag ctgggtatga gagaggcagg ggccgagccg gcgcggtgac
     1321 atgctcgccg ggcggcgccg gtggccgaga ccggcaagca cggccgcatc cagcggccga
     1381 tccttgcgat agcgcacgtc gtccccgccg attttgcgct aacagtacgt attacagtca
     1441 atttgcatga cgcctcattt gatatataat caatttccac gacgaccgaa gacccgcacg
     1501 atcggctgga cgctgcgtcg agtgcggagc agagaaagcg atgccacagg cagagtctca
     1561 aatattgagc gcgtacattg ccaacgagac ggccatcaag cgcttcctgg gccgctttct
     1621 gaagaacgtc caggatatag acgacactgc gcaagaggtg ttcatgcggg cgttcgtggc
     1681 cgatgccatc gagccggtcg tcactccgcg accttaccta ttccgcgtcg ctaagaacct
     1741 ggcgctcaat caccttgcca agctttccaa tctgctgacc gaccggatag aggatgtttc
     1801 cgacacatcg gcgttgccta ctgaccatca ggtgagcatc gaggatgagg tggatgccgg
     1861 ccagcgcttc gggaatgtcg ttcgcgcaat tggcgcattg ccgccaagat gcgcaaaggt
     1921 tttcgtcctg cggaaaatcc acgggttctc gcagaaggag attgccttgc agctgtgcat
     1981 ctccgaaagc accgtcgaga agcatattgc gctgggcttg gtgcgatgtg gcgaatactt
     2041 gcgacagcag ggcggggcgc tgaattggga atgagcagcg tgcgggcgac ggagagcagc
     2101 tgggctcagt cgaatcgttc gagctcgcgc tgattcctgg atgcttgcaa gccggcgacc
     2161 gcacgcgcgc ataccgaaag atcctgaatc gccgttccag agctgtcgaa caccgtgagc
     2221 tcgtccgggg acgaacggcc cggcgcctcg ccgcgtatca cggctcccag cgtcgtcagc
     2281 cgcgcacagt cgagcaggcc ggcgcggtac gcgctctggc actcgccgat cgtcacgctc
     2341 tgtgcgatca catccacgaa gactcgtgcc cgcgcaagca attcagtcgg taattcctgc
     2401 tttccaggcg catcggcacc catggccgat atgtgtgccc caggggaaac ccattccggc
     2461 gcgaacaagg gcccgatagc cgccgtggcg gtgacgacga tgtcggcgcc atcgagcgca
     2521 ttctgcaatg ccaacggacg ggccgggtag cctgccgcac atgcgcgggc cgcaaattcc
     2581 cgggcgcgct cgggattgcg gttccacact cgaatgtctt gcagcgcccg cacgaggccg
     2641 atcgcctgca ggtcgaacca cgcctgatga ccggaaccaa ccagcgcgag ccgcgaggcg
     2701 tccgggcgag caaggtgcct gacgccaatt gcgtccgcgg ctgccgtgcg cagtgccgtc
     2761 aaatatgaag cgcgaatgac tgccagggga tgacctgtct gcggatcgag tagaagcacc
     2821 gtggacgcgt gcgcttcgag tccgcgctcg agattgtgcg gccagtaggt tccaatcttg
     2881 catccaatcg ttccggtgtc gctgcgtccg gccttgatcg cccaccgctg ct
//
LOCUS       DASZMC010000270         3740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_681290,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000270 DASZMC010000000
VERSION     DASZMC010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681290"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1323
                     /locus_tag="VG274_05495"
     CDS             385..1323
                     /locus_tag="VG274_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWE06141.1"
                     /translation="MVTMAPKTVMPGTVVSRNDSQLASEVEGRIASVAEVGTVVPKGG
                     VVARVDSNLAGMQLASDKASVARLDAQLRFDRNQAQRLDSLFVQNAIAKSTRDQAEST
                     RDMDAAALAQAEAAARKSQYQFDHSDIRAPFPGRVVARLINPGEYATAGKPIVRLVDT
                     GSIEASVQIPIETASYLRERMPVTVEIESRRATAVVRAIVPVGDIASRTIEVRLTLPP
                     GTGFVGDAAKVFIPTAQPRDVVAVPRDALVLREDNTYVFKVGRKGVAERVAVETGSED
                     GGLVEIKGPVSPGERVIVRGAERLEAGQKVRPILAS"
     gene            1736..3604
                     /locus_tag="VG274_05500"
     CDS             1736..3604
                     /locus_tag="VG274_05500"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWE06142.1"
                     /translation="MPNIRKLPSAVWQGIILISLPALILVALQIYSAARIVPGSWRSQ
                     QLVAHTLHVIDAARALDQSMSETERGEFGYIVTGDRTYLDIYQGGARRTPQNLQRLRQ
                     LTQDNPDQTRRISLLATAVDLKLTELQSAVEARQKKGFIAAYRLIEANLKPDTRRVIT
                     GLVELTITAEDKLLAAREAKLAMLENSAENASAIGIAMTVSLMLLGALMLARALRHDA
                     RRLARLRESEERFRLLVSGVKDYAIFMLDPEGRVVSWNEGARRIKGYSPVEAIGLHMS
                     AFYPPEIGNRRGVVDEELAVAAREGSFEAEGWRVRKDGSRFYANAVITALRADDGALR
                     GFAKVTRDVTEKRSHEAAIEESRSALVQAQKMEALGQLTGGLAHDFNNLLSVIIGGID
                     LAQRDMDPPDPARNARMLDAAKQAAQQGAALVRRMLAFSRRQKLAPQIVDINSSVRSM
                     AELIRRTLGEPVKLECILEPALWRTRADANQLESALLNLCVNARDAMPQGGTLAIVTN
                     NFFQSARAQFDTPPGEYVVLSVSDTGEGMAPEIVAHAFEPLYTTKPEGKGSGLGLSQV
                     YAFAKQSGGRATIESAIGRGTTVKIFLPRFVEEAMDAELTNQTDTGRSPNVRTESG"
BASE COUNT          744 a         1139 c         1202 g          655 t
ORIGIN      
        1 cgttgacgca accgcctttg tgcctggcag gaatcgtatt gatcttacct cccccttgcg
       61 gggaggtcga aatttgcgaa gcaaattttg ggtgggggtc tcggcctctc catggatggg
      121 cggcgacccc catccgaaaa acgcgaagag cgtttttcga cctccccaca agggggaggt
      181 taagcgtatc ggcgagcgca aacgatttga tttgaacgca ttgcgctccg gtttggcgtt
      241 tgcgggaggt tcgccgccgt gattggaagg aaatcaggaa gtaccttcgc aatcgcgtgc
      301 ctgtcagcgg gcctcgcact atcgccgcaa gcgctggcgc agcaacgccc cgatccgcct
      361 tcggttggcg taattcccgc gcggatggtg acgatggcgc cgaagacggt gatgccgggc
      421 acggtcgtct cgcgcaatga ttcccagctg gcatccgaag tcgagggccg catcgcttcg
      481 gtcgccgagg ttggaacggt agtgcccaag ggtggcgtcg tggcgcgggt ggacagcaat
      541 ctggcgggca tgcagcttgc ctccgacaag gcgagcgttg cgcggctgga tgcgcagctt
      601 cgtttcgacc gcaaccaggc gcagcggctc gacagtctct tcgtgcagaa tgcaattgca
      661 aagagtacgc gcgatcaggc cgagtcgaca cgggacatgg acgcagcggc gctggcgcag
      721 gcggaagccg ccgcgcgcaa gagccagtac cagttcgatc acagcgacat ccgcgctcca
      781 tttcccggcc gcgtcgtggc gcggctgatc aatcctggcg aatatgcgac ggccggcaag
      841 ccgatcgtcc gtcttgtcga cacaggctcg atcgaagcga gcgtccagat accgatcgaa
      901 acggccagct atctgcgcga gcgaatgccc gtgacggtcg aaatcgagag caggcgcgcg
      961 acggcggttg tccgcgcaat cgtaccggtt ggggacatcg ccagccgtac gatcgaagtc
     1021 cggctgacgc ttccgcccgg cacgggtttc gtcggcgacg ccgcaaaggt cttcattccc
     1081 acggcacagc ctcgcgacgt ggtggccgtg ccgcgcgatg cgctcgtgct gcgcgaggac
     1141 aacacctacg tcttcaaggt gggtcgcaag ggcgtggcgg agcgggtggc cgtcgaaacc
     1201 ggatcggaag atggcggact ggtcgaaatc aagggaccgg tctcccccgg cgagcgtgtc
     1261 atcgtgcgcg gcgccgagcg gctcgaagcg ggtcaaaaag tgcggccgat cctcgcttcg
     1321 taacgcgaag tgttgggggg aaaaaggcgt tcctggtgga ccgattctga cacttggtac
     1381 ccaaaatttg tcatggcccg gtttaaccgg gccacccagc cagcgcgcgt cggcgcgctg
     1441 attagacgtg ccgagtggtt tcgtctgggc ggcccgctcg gagtttacac tccgaccgcg
     1501 cactcgcgcg gatcggggtg gcgggccatg acaatttata gggattcaac tgtcggacct
     1561 gcaccactct catggttcgg caagctcacg atgagggcct catcctgagc ctgtcgaagg
     1621 atgcgcccgc ttgcccctct aatgcatccc gttatgctca atccgggctg agctgcccgg
     1681 tcgaagacgc gcgaacggac acgcaagggg tccacgcgcc catgagtgtg gggctgtgcc
     1741 gaacattcgc aaactgccca gtgcggtgtg gcaggggatc attctgatct ccctgccggc
     1801 gctgatactg gttgcccttc agatctacag cgcggccagg atcgtccccg gctcgtggcg
     1861 cagccagcaa ttggtcgcac ataccttgca cgtcatcgac gcggcgcggg ctctcgacca
     1921 gtcgatgagc gagaccgaaa ggggcgagtt cggatacatc gtcaccggcg accggaccta
     1981 tctcgatatc tatcagggcg gagcgcgccg aacgccgcag aaccttcaac gcctgcgcca
     2041 gctcacgcag gacaaccctg accagacgcg gcgcatctcg ctcctggcaa ccgcggtcga
     2101 tctcaaactc accgaactgc aaagcgccgt cgaagcacgc cagaaaaagg ggttcatcgc
     2161 ggcgtatcga ctgatcgagg cgaacctgaa gccggacacg cgccgggtca tcaccgggct
     2221 ggtcgaactc accatcaccg cagaagacaa gctgctcgcc gcccgcgagg caaaactcgc
     2281 gatgctcgaa aacagcgccg agaatgcgag cgccattggc atcgcgatga cggtgtcgtt
     2341 gatgctgctc ggcgccctca tgcttgctcg cgccctgcgc catgacgccc ggagactcgc
     2401 ccgtcttcgc gagagcgaag agcgtttccg tctccttgta tccggggtca aggactacgc
     2461 catattcatg ctcgatcctg aaggccgcgt ggtcagctgg aatgagggcg cgagacgcat
     2521 caagggctac agccccgtgg aggcgatcgg attgcacatg tccgccttct atccccccga
     2581 aatcggcaac cgccgcggtg tcgtcgacga agagctcgcc gttgcagccc gcgagggaag
     2641 tttcgaggcg gagggctggc gcgtccgcaa ggatggatcc cgcttttacg caaacgcggt
     2701 aatcaccgcg cttcgcgcag atgatggagc gttgcgcgga ttcgcgaagg tcacgcgcga
     2761 cgtgacggaa aaacgctcgc acgaggccgc gatcgaagaa agccgctccg cgctggtgca
     2821 ggcgcagaag atggaagccc tggggcagct gaccggcggc ctcgcacatg atttcaacaa
     2881 cctgctgagt gtcatcatcg gaggaatcga tctcgcacaa cgggatatgg atcccccgga
     2941 tccagcccgc aacgcgcgga tgctcgatgc ggcaaagcag gctgcgcagc agggcgcggc
     3001 gctcgtgcgc cggatgctgg cattttcccg gcgccagaag cttgcgccgc agatcgtcga
     3061 catcaattca tcggttcgca gcatggcgga actgatccgg cgcacgcttg gcgaacctgt
     3121 gaagctggaa tgcattctgg aacctgcctt gtggcggacg cgcgcggatg ccaatcagct
     3181 cgaaagcgcg ctcctgaacc tgtgcgtgaa cgccagggat gcgatgccgc agggcggcac
     3241 gcttgcgatc gtcacgaaca atttcttcca gagtgcaaga gcccagttcg atactccgcc
     3301 cggagaatat gtcgtgctat cggtgagcga taccggcgaa ggcatggcgc cggagattgt
     3361 cgcgcacgcc ttcgagccct tgtacacgac caaacccgaa ggcaagggat cgggcctcgg
     3421 actgagccag gtgtacgctt ttgccaagca atccgggggc agagccacca tcgaaagcgc
     3481 aatcggccgc ggcacgacgg tcaagatatt ccttccgcga tttgttgagg aagcaatgga
     3541 tgcggaattg accaaccaaa cagatacggg gagatcgcct aacgtacgga cagaatcggg
     3601 ctgattctag agcgtgttcc gttcccatta aaccaaacat gtcattcccg ccgaagcggg
     3661 aatccagtgg caaccgcacg tcgctttgcc ctggattccc gcttcggcgg gaatgacaag
     3721 tgcggttatg gagctaacga
//
LOCUS       DASZMC010000271         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_681324,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000271 DASZMC010000000
VERSION     DASZMC010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681324"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..>2718)
                     /locus_tag="VG274_05505"
     CDS             complement(133..>2718)
                     /locus_tag="VG274_05505"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWE06143.1"
                     /translation="GAFRAGSRPDAEPYCIMIPPPNVTGRLHIGHALNNTLQDILARF
                     ERMRGRDVLWQPGTDHAGISTQLVVERNLAERQQSRLALGREKFLAEVWKWKEESGGA
                     IVQQLRRLGASCDWSRERFTLDEGLSRAVAKVFVQLYREGLIYKDKRLVNWDPRLQTA
                     VSDLEVENIEVKGHLWYIKYPIDGEPGRFITIATTRPETMLADAAVAVHPDDERYRGV
                     IGKTALLPLANRRIPIIADSYSDPEKGTGAVKITPAHDFNDFEVGRRHGLPLIDILLR
                     DGRLNDAVPAAYQGLDRFEARKAVVADLEALELIERVEPIVHAVPHDEKTKTIALEPY
                     LTEQWYLDVRPLADKAIDAVERGQTRFVPDQWSNVYFNWMRAIRPWCISRQLWWGHQL
                     PVWYDKSGNVYVAETEAEAVAEAHRINGAATELERDPDVLDTWFSSALWPFSTLGWPD
                     ATPELARYYPTNVLVTSFDIIFFWVARMMMMGLHFRHDVPFRTVFIHTRVLDEKGQKM
                     SKTKGNVVDPLVLIDEFGADALRFTLALAAGQGRDMRIGPSRVESSRNFSTKLWNAAR
                     FCEMNGCETREDFAPENAASTLNKWIVAETAAAVRRVTQALEELRFNDAANLAYHFVW
                     SRFCDWYLELVKTVFSAGDAAETAETRATAAWVRDQILGLLHPFMPFITEELWGRTAE
                     RGREFDLLLIRTAWPQPAAQPDAEACGEIEWLIELVSGVRSVRMEANVPAAAKIGLVL
                     RGANAVTRSRLERNRDAVTALARLSSVSFADAIPRDSAQFVIREAVAALPLGDVIDLE
                     KERARLRKELQRAELEIARFDTKLGNADFLARAPEDVVDEQKERRADAEALASRLRDA
                     VTHLG"
BASE COUNT          484 a          872 c          845 g          517 t
ORIGIN      
        1 tcgttagctc cataaccgca cttgtcattc ccgccgaagc gggaatccag ggcaaagcga
       61 cgtgcggttg ccactggatt cccgcttcgg cgggaatgac aagtgcggtt atggagctaa
      121 cgaaactcgg ccctacccca ggtgggtcac cgcgtcgcgc aggcgcgatg ccagggcttc
      181 tgcgtccgcc cgccgctcct tctgctcgtc gacgacatcc tcgggcgcgc gggcgaggaa
      241 atcggcattg ccgagcttgg tatcaaacct cgcgatttcc aattcggcgc gttgcagctc
      301 cttgcgcagc cgggcccgct ccttttccag atcgatgacg tcgccaagag gtagcgccgc
      361 caccgcttcc cggatgacga attgcgccga gtcgcgcgga atcgcatcgg caaacgacac
      421 tgaggagaga cgcgccagcg cggtgactgc gtcccgattg cgttcaagcc gggagcgcgt
      481 caccgcgttg gcaccgcgta gcacaagccc gatcttcgcg gccgcgggca cgttcgcctc
      541 catgcgcacc gagcgcacgc ccgatacgag ctcgatgagc cattcaatct cgccgcatgc
      601 ttccgcatcg ggttgcgcgg cgggctgcgg ccatgcggtc cggatcagca gcaaatcgaa
      661 ctccctgccc cgctcggccg tgcggcccca aagctcctcg gtaatgaacg gcatgaaagg
      721 atgcagaagc ccgagaatct gatcgcgcac ccaggcggcg gtggcgcgcg tttcggccgt
      781 ctcggctgca tcgccggcgc tgaaaaccgt tttgaccagc tcgagatacc agtcgcaaaa
      841 ccgcgaccag acaaaatggt aggcaagatt tgccgcatcg ttgaagcgca gttcttccag
      901 ggcctgggtg actctccgta ccgctgcggc agtttcggcg acgatccatt tgttgagggt
      961 cgatgccgcg ttttctggag cgaagtcctc gcgcgtttcg cacccgttca tttcgcagaa
     1021 gcgcgcggca ttccacagct tggtcgagaa attgcggctg gattcgaccc gtgatggtcc
     1081 gatgcgcata tcccgcccct gcccggccgc cagcgccagc gtaaagcgca gcgcgtcggc
     1141 gccgaattcg tcgatgagaa ccagcggatc cacgacgttg ccctttgtct tcgacatctt
     1201 ctgccccttc tcgtcgagca cgcgcgtgtg gatgaacacg gtgcggaagg gaacgtcgtg
     1261 gcggaaatgc agacccatca tcatcatccg cgcgacccag aagaagatga tgtcgaagct
     1321 cgtaaccagc acattcgtcg gatagtagcg cgccagttcg ggtgtcgcgt cgggccagcc
     1381 caaagtggag aacggccaca gggccgaaga gaaccaggta tcgagcacgt cgggatcgcg
     1441 ctcgagttcc gtcgccgctc cattgatccg gtgcgcttcg gcgacggctt cggcttccgt
     1501 ctccgcgacg tagacgttgc cggatttgtc gtaccacacg ggcagctgat gcccccacca
     1561 gagctggcgc gagatgcacc aggggcggat ggcgcgcatc cagttgaaat aaacgttgga
     1621 ccattggtcg ggcacgaagc gcgtttgtcc ccgctcgacg gcgtcaatcg ctttgtcggc
     1681 cagcggcctc acatcgagat accattgctc cgtgagataa ggctcgagcg cgattgtctt
     1741 cgtcttctcg tcatgcggca ccgcatgcac gatcggctcg acccgctcga tcagttcgag
     1801 cgcctccaga tctgcgacga cggctttgcg cgcttcgaaa cgatcgagcc cctgataggc
     1861 ggccgggacc gcgtcgttca atctgccgtc ccgcagcagg atgtcgatca gcggcaaccc
     1921 gtgccgccgg ccgacttcga aatcgttgaa atcgtgcgcg ggcgtgatct tgaccgcgcc
     1981 tgtgcctttt tccggatcgg aataggaatc ggcaatgatc ggaatccggc ggttggcgag
     2041 cggcagcagc gcagtcttgc cgatgactcc gcggtagcgc tcgtcgtccg gatgcacggc
     2101 gacggctgca tcggcgagca ttgtttcggg gcgtgtcgtg gcgatcgtga tgaagcggcc
     2161 gggctcgccg tcgatcggat atttgatgta ccagagatgg cccttgacct cgatgttctc
     2221 gacttcaagg tcggagaccg cggtctgcag gcgcggatcc caattgacga ggcgcttgtc
     2281 cttgtagatc agcccttcgc gatagagctg aacgaaaacc ttcgccaccg cgcgcgacag
     2341 gccttcatcc agcgtgaatc gttcgcgcga ccagtcgcat gaggcgccca gccttcgcag
     2401 ctgttgaacg attgcgccgc ccgactcctc cttccatttc cagacctcgg ccagaaactt
     2461 ctcgcgaccg agggcgaggc ggctctgctg ccgctccgcc agatttcgct ccacgaccag
     2521 ctgggtggaa ataccggcat ggtcggtgcc cggctgccag agcacgtccc tgccccgcat
     2581 gcgctcgaag cgcgcgagga tatcctggag cgtgttgttg agcgcgtgcc cgatatgcag
     2641 ccgcccggtg acgttgggtg gcggaatcat gatgcagtat ggttccgcgt ccggtctcga
     2701 tccggcacgg aacgctcc
//
LOCUS       DASZMC010000272        14705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5336695,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000272 DASZMC010000000
VERSION     DASZMC010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 14705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14705
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5336695"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..939
                     /locus_tag="VG274_05510"
     CDS             94..939
                     /locus_tag="VG274_05510"
                     /inference="COORDINATES: protein motif:HMM:NF019947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWE06144.1"
                     /translation="MLGTEWIGSVLTDLAASSLFVAGGFVVGRLQERRRLTGRPLHDY
                     DFYPYAATRENFAEFSLKDFRLAVHHFLRNTDSRAARQLIFIGEQNNVKDQLNASDLR
                     AYERLLAKYHGAGVTDDTREFLENYHNIARLLGRTFPNMGIEVLVHDLSNPSHSITTI
                     ESGEVTGRVVGMGTTALLVDLKRRVNLGQDKLNYELNIGARRFKCTTIPIVRKEFGVV
                     GAICINIDINFIRDEVQPSPERIVEFFRNYCRVDMKLEENILSKEEYRKALAGKKQFR
                     DFTAA"
     gene            1161..1718
                     /locus_tag="VG274_05515"
     CDS             1161..1718
                     /locus_tag="VG274_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HWE06145.1"
                     /translation="MTRFSIEELLETGLFKSRWILAPFYLGLILALVMLLVAFINELV
                     HTLPNVFTLDVEQIILSVLTLIDLSLAGNLVLIVIFSGYENFVSKIDTHGSEDRPAWM
                     GTLDFSGLKMKLIGSIVAISAISLLRAFMSVLEPEVGLDERRLVWLVVLHFTFLMSGV
                     LFAFMDWLAAVTEKHVRKEVRVHGS"
     gene            complement(2017..3597)
                     /locus_tag="VG274_05520"
     CDS             complement(2017..3597)
                     /locus_tag="VG274_05520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWE06146.1"
                     /translation="MTTDTNAGAQAGVEEATAATSEILPNSETDFPGFEHLGLSPSVL
                     KAVAESGYTKPTPIQARGIPLVLKHRDLIGIAQTGTGKTASFTLPMIEILARGRAKAR
                     MPRSLILEPTRELAAQVAESFERYGKYNKLSMALLIGGVSFDDQDKKLDRGVDVLIAT
                     PGRLLDHFERGKLMLSQVQVLVVDEADRMLDMGFIPDVERICKLIPFTRQTLFYSATM
                     PPEIKRLTDQFLHNPEQVEVARPATTVASLAQEIVEVPANDWAKREALRRLVRENPVN
                     NAIVFCNRKRDVDVVARSLQKHGFSVAPLHGDLDQAIRTKTLDSFRNGEIQLLVASDV
                     AARGLDIPAVSHIFNFDVPFHPDDYVHRVGRTGRAGRQGHALMLATPRDAKLVDAIEQ
                     LIGQPIGSRKMENLEVREDLGHSRGRHQGERSAHGRRPSRAPRGQSHAPASAKGHHVP
                     AASEHAAKPHQQPPTAKTSESPPPRREQPPTPPRLAREHDAPDLNQLPAFLLRPVKLP
                     PSEKSAARKPKKVAEATP"
     gene            3803..4183
                     /locus_tag="VG274_05525"
     CDS             3803..4183
                     /locus_tag="VG274_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE06147.1"
                     /translation="MVKLDHLTLAVGDWRVSRDWYVGHLGFKVEFEVPHGGSSGLGAV
                     AIQDDSGFTVFLEQFDKPVLSGQSSCTIQVDEVDALFQRLKGSVKVIAEPGRQFWGYG
                     AVIADADRHLLHLYDTTSMNEKDG"
     gene            4257..6587
                     /locus_tag="VG274_05530"
     CDS             4257..6587
                     /locus_tag="VG274_05530"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWE06148.1"
                     /translation="MIDNPSRAMGQLNTPKTGLLRLFITIALLAVAASFWFLQIYGSA
                     RPQVAPLDASPRAFSAARAEHVLAQLLEPEIPHPVSSDENANVRARIVQELSVLRVPA
                     FVLRSFACHAMHSYGVLTCATVNDILAVVKPGQGRAIVLLAHYDSVPAGPGAADDESG
                     VATVIETIRALLTRGLKSRHPILAVLTDGEEAGLLGAAAFLHEPALKARVGAVVNVEA
                     RGNQGPSLLFQTSPGDGPLIDLYANNVPDYATSSVYAEIYRFLPNDTDLTLFIRDGFP
                     SFNFAFAGNIADYHTALDRRVNLDPVSLQEQGDNMLGVASGLEQADFAKLRGSDDIYL
                     DILGRVLPRVPAAWDLPLSLAAFVLLLAATLLARREPISWRRWLCAFAITPALVLVAA
                     GSGFALQAIAAFVAGTPDPSYAHPAALRIALAFGLAGATLLVSRLTLPGYAPTAVWLW
                     IAALGIVVAIFLPGLSPYFLIPASAAAILLLATACIPGGWDSAAGAIAVFAAALVALL
                     VWSSISASGESVMGLKLHPLFTVPFAIGLTTLVPLFMRYAMPRRLWATATTTLFVAAL
                     CGAVVQGLEPAYSSTAAQRLNVTYVEDRHRALWAVDTLAPVPGEMREIVSFASEPQRL
                     SRAMPLAYVAPAGAPRFPLPTATIASDRYVGAGRSLTLVLHGSRKAAQMILVIPRSAM
                     LKAIDLSGRHFDAPPSWANEDSVALACMSRDCASTTVTLTLASRSTQALELYEHRFGL
                     PDFAKHLVAARPPTAVQSQNGDGVVLMNKILVPALR"
     gene            complement(6728..8701)
                     /gene="parE"
                     /locus_tag="VG274_05535"
     CDS             complement(6728..8701)
                     /gene="parE"
                     /locus_tag="VG274_05535"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HWE06149.1"
                     /translation="MSKSPDLFEGAPAPSKAYTAKDIEVLEGLEPVRRRPGMYIGGTD
                     EHALHHLAAEILDNAMDEAVAGHATRIEIDLKPDDVLTMRDNGRGIPVDPHPKFKNKS
                     ALEVIMTTLHAGGKFGGKVYDTSGGLHGVGASVVNALSEWMEVEVCRERHAFRMRFAR
                     GHAVGKLKDAGAVNRRGTVVSFKPDEQIFGKSVHFSPRALYRMARSKAYLFRGVEIRW
                     SCAPELLKGDGGTPAEETLKFPGGIVDFLNSEIEGRAAVTSRNFYGRSENRDGKGAVE
                     WAVSWVPEADPFIHSYCNTIPTTQGGTHEQGFRNALTKALRAYGERANNRKAALIAPD
                     DVMETACAVLSIFIREPEFQGQTKEKLSTPDAQRLVENVVRDHFDHWLAEHPPEADKL
                     LAFIVDQAEERLRRKQEREVSRKTATRKLRLPGKLADCSRDNASGTEIFLVEGDSAGG
                     SAKQARDRATQAVLPLRGKILNVASAATGKLEQNQELANLVQALGCGTGARYRDEELR
                     YERVIIMTDADVDGAHIASLLLTFFYREMPGLIANGHLHLALPPLYRLTQGGKIVYAR
                     DDAHREQLLETTFKPGAKIEVSRFKGLGEMMPNQLKETTMKPGMRTLERVEIADGERE
                     ETATLVERLMGKRPELRFQFIQENARFVGEVDL"
     gene            complement(8889..10298)
                     /gene="glnA"
                     /locus_tag="VG274_05540"
     CDS             complement(8889..10298)
                     /gene="glnA"
                     /locus_tag="VG274_05540"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HWE06150.1"
                     /translation="MANASSVLKLIKEKEVKFVDLRFTDPRGKWQHVTYDLSLVDDDF
                     LTNGTMFDGSSIAGWKAINESDMTLLPDCDTAIIDPFFAQTTLAMFCDIIEPTTGQRY
                     ARCPRSIAKAAEKFMASSGVGDTAYFGPEAEFFIFDDVRFDSTMNKGFYYIDSAEGAY
                     NSGTEFEGGNLGHRPAVKGGYFPVPPVDSAQDIRSEMLAVMGDLGIEPEKHHHEVACA
                     QHELGFKFKTLTRCGDAMQIFKYVVHQVAAAYGKTATFMPKPVYGDNGSGMHVHQSIW
                     KGGKPLFAGSGYADLSDTCLFYIGGIIRHAKAVNAFTNPLTNSYKRLIPGFEAPVLLA
                     YSSRNRSASCRIPFSASPNGKRIEVRFPDPGANPYLAYAAMLMAGLDGIENKIHPGDP
                     MDKDLYALPPEELRDVPTVCGSLRQALECVDAGRAFLKKGGVFSDDFIDSYIELKMGE
                     VHAFEHTPHPIEFKMYYSV"
     gene            complement(10341..10679)
                     /locus_tag="VG274_05545"
     CDS             complement(10341..10679)
                     /locus_tag="VG274_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWE06151.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEIGVQGITVVEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVPADRLEAALEAIQKAARTGRIGDGKIFVFDIEQAIRI
                     RTGETGADAI"
     gene            10920..12140
                     /locus_tag="VG274_05550"
     CDS             10920..12140
                     /locus_tag="VG274_05550"
                     /inference="COORDINATES: protein motif:HMM:NF006488.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HWE06152.1"
                     /translation="MSLSKQDGMPKGFGMSGMRFNPVFSGLPTTIFTVMSALAVEHKA
                     INLGQGFPDEDGPLAIREAAAAALREGPNQYPPTRGLPALRGAIAAHAKRFYGFDFDP
                     ERNILVTSGGTEALTASILALAGEGGEVVLFEPAYDSYRPIALAAGALVRSVKLAPPG
                     WRIEEQALAAAITSKTRIVMLNTPLNPIGRVFDGDELAMVARVLAHSHAVVICDEVYE
                     HLVFDGRPHLPLIGFPGMEERVVRVGSAGKMFSLTGWKVGWVTGAAPLIDVIAKAHQF
                     ITFTTPPALQRGTTYALSNEMDFTLMLTRTLQDNRDLLAGRLARMGFDVLPCEGTYFL
                     TAGIGALTNERDDDFCRRLVREAGVALIPLSAFFETGEPGHLVRFAFCKKRALLEEAM
                     NRLERHFAGQERVR"
     gene            12196..13686
                     /locus_tag="VG274_05555"
     CDS             12196..13686
                     /locus_tag="VG274_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWE06153.1"
                     /translation="MSQEILTVAEMYAADRYAGENGVPSLTLMENAGRAIAMEITRRW
                     SARPTFVLCGPGNNGGDGFVVARHLAERGWEARVALLGDRDRLMGDAAAMATRWAGEV
                     LPLSHDLVSDASLVVDAMFGTGLARPLEGVVGETALALNASDAVVVAVDVPSGVQGDL
                     ARPIDGPKGVAVEADLTVTFFRKKPAHVLMPGRLRCGEVTVADIGIPPAALETIRPRM
                     FENAPALWGTGYPWPQPLAHKYARGHALVVSGPAHATGAARLAARAALRAGAGLVSVA
                     SPLDAIAVNAASLTAVMVKPFSGVAGLADLLKDARFNAIVIGPGCGVGRTTQEMVAAV
                     LASNAAVALDADALTSFAGEPDTLFRQLRDPAVLTPHAGEFARVFPDVLKRTPTRIEA
                     AREAAAVARCTVLLKGPDTVIAAADGRIAVTTNAPPTLATAGAGDVLSGIITGLLAQG
                     MDSFSAAAAGAWLHGEAASRSGYGLIAEDLAEILPAVLSSLRDTPA"
     gene            13683..14207
                     /locus_tag="VG274_05560"
     CDS             13683..14207
                     /locus_tag="VG274_05560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE06154.1"
                     /translation="MSTPQVRRALASDMSALLGIYNHYVFHTHITFDIEPRTLEQRLE
                     WFAGFSSAGRHQCFVAVEGGMPIGWASSSPFKERAAYDTSVETSVYLAPDATGRGIGR
                     KLYETLFEALQHADAHRAYGGIAQPNEASVRLHERMGFSCMGTYHEVGRKFGRFWDVA
                     VYERPLGREPLVTP"
     gene            14263..14347
                     /locus_tag="VG274_05565"
     tRNA            14263..14347
                     /locus_tag="VG274_05565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14297..14299,aa:Leu,seq:tag)
     gene            14384..>14705
                     /locus_tag="VG274_05570"
     CDS             14384..>14705
                     /locus_tag="VG274_05570"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HWE06155.1"
                     /translation="MQITETVSEGLRREFKVVIGAGDLDARLTGRLEEMKSRVHLKGF
                     RPGKAPVSYLKKTYGKSMMGEIVEQAVSESSQKTGEDHALKLAFPPQVDLVSPIEEVV
                     GGKSD"
BASE COUNT         2587 a         4457 c         4687 g         2974 t
ORIGIN      
        1 agatcacctt acgggccggc gccagccggc ggaagcggtg cgccaggtac ggaagttgct
       61 aagataggtt cgaggcggca ggagagggcg tccatgctcg gaacggaatg gatcggatcg
      121 gtgctgaccg atttggctgc gtcgtcgcta ttcgtggcgg gagggttcgt cgtcggtcgc
      181 ctgcaggaaa ggcggcgcct gaccggacgg ccgctccacg actacgattt ctatccctat
      241 gcggcgacgc gggagaattt tgccgaattc agcctcaagg atttccggct ggccgtgcat
      301 cacttcctgc gaaacactga tagccgcgca gcacggcagc tcatcttcat cggcgagcag
      361 aacaacgtga aggatcagct gaacgcgagc gaccttcgcg cctatgaacg cctgctggcg
      421 aagtatcacg gcgccggcgt taccgacgac acgcgcgagt tcctcgagaa ctaccacaat
      481 atcgcacgcc ttctcggccg cacctttccc aacatgggca tcgaggtgct ggttcacgat
      541 ctgtcgaatc cttcccattc catcaccacg atcgaaagcg gagaggtaac cggacgcgtt
      601 gtcggcatgg gcaccacggc tttgctcgtc gacctcaagc gccgcgtcaa tctgggacag
      661 gacaagctga actacgagct gaacatcggc gcgcgccggt tcaaatgcac cacgattccg
      721 atcgtgcgga aggaattcgg cgtggtcggc gcgatctgca tcaacattga catcaatttc
      781 atccgcgacg aagtacagcc gtcgccggaa cgaatcgtgg agttcttccg caactactgc
      841 cgcgtcgaca tgaagctgga ggagaacatc ttgagcaaag aggagtatcg caaggcgctc
      901 gcggggaaga agcaattccg cgattttacg gcggcgtgac gcccgccaaa caggcaagca
      961 ataccttgat ccacgctttt ttcctccccg cgcgcaaagc gcgcggggca ggtgccccga
     1021 atggtcggat ttcacccgac cacgaagcgc cggaggggga aagtgggggc ggcgggctct
     1081 ccccgcgaaa ggcggcggag gaaaggtgac ggcagggctg cgtcgtgatg gaatctgggg
     1141 ccgattcgcg gtggacggtc atgaccaggt tttccatcga agaactcctc gagaccggac
     1201 ttttcaaatc gcgatggata ctggcgccgt tttatctcgg cttgatcctc gcgctcgtga
     1261 tgctgcttgt cgcgtttatc aatgaactcg tgcacacgct tccgaacgtc ttcacgctcg
     1321 atgttgagca gatcattctt tccgtgctga ccctgatcga tctgtcgctt gcgggcaacc
     1381 tggtcctcat cgtgattttc tcgggctacg agaacttcgt ttctaagatc gatacgcatg
     1441 gcagcgagga tcggcccgcc tggatgggca cgctcgattt ctccgggctg aagatgaagc
     1501 tgatcggctc gatcgtcgcg atttccgcga tttcgctcct gcgtgccttc atgagcgtgc
     1561 tggagcccga agtggggctc gatgagcgca ggctggtctg gctcgtcgtg cttcatttca
     1621 cattcctcat gtcgggcgtc ctgttcgcct tcatggactg gctcgccgcg gtgaccgaaa
     1681 agcacgtgcg gaaggaagtg cgggtgcacg gatcgtagag atcgccttta cgcactgggc
     1741 cgcaagaaag agcgtcatgg cctgcgcgtg aagcttgtcc tccggccgcg cttcgcgcgg
     1801 accgggggga gcgcctagat ggaagcggtg cggcgccggg aggactacaa gaacatgcac
     1861 gcaatcggcg ccgtccaaat tacagctggg tggcccgcat ggagtttaca ctccgaccgc
     1921 gctctcgcgc gcatcggggt ggcggcccat gacaaattat ggaatccggc cacttacgca
     1981 ctgcgcgcga tgcgagtttg gggccgccaa tgacgcttac ggcgtcgctt cggcaacttt
     2041 cttcggcttg cgcgcggcgg atttctcgct cggcggtagc ttgaccggcc tcaacagaaa
     2101 cgccggaagc tggttgagat ccggcgcgtc gtgctcgcgc gccagccgtg gcggcgtggg
     2161 cggttgctct cgccggggcg gaggtgattc ggaagtcttg gcagttggcg gttgctgatg
     2221 tggtttcgcc gcgtgctcgc ttgcagccgg cacgtggtgg cccttggcgc tcgccggcgc
     2281 atggctctgg ccgcgcggcg cgcgagaggg cctgcgcccg tgcgcgctac gctcgccttg
     2341 atgacggccg cgcgaatgtc ccaaatcctc gcggacctca agattttcca tctttcggct
     2401 gccgatcggc tggccgatca gttgctcgat cgcgtccacg agtttcgcgt ctcgcggtgt
     2461 cgccagcatg agtgcatggc cctggcgtcc cgcccggccg gtgcgtccga cccggtgcac
     2521 gtaatcatcc gggtgaaaag ggacgtcaaa attgaaaatg tgcgagacgg cgggaatgtc
     2581 gagaccacgg gccgccacgt cgctcgcaac cagaagctgg atttccccgt tgcggaacga
     2641 atcgagcgtc ttcgtgcgga tggcctgatc gagatcaccg tgcaacggcg ccaccgaaaa
     2701 tccgtgcttc tgcagcgagc gggccaccac gtcgacgtcg cgtttgcggt tgcagaagac
     2761 gatggcattg ttcaccggat tctcgcgcac cagtcgccgc agcgcttcgc gcttggccca
     2821 atcgttggcg ggcacttcga cgatctcctg cgcgaggctg gccaccgtgg tcgccggccg
     2881 cgccacttcg acctgctcgg gattgtgcag gaactggtcg gtgaggcgct tgatctcggg
     2941 cggcatggtc gccgaataga acagcgtctg ccgcgtgaac gggatcagct tgcagatgcg
     3001 ctcgacgtcg gggataaacc ccatgtcgag catgcggtcg gcctcgtcga cgaccagcac
     3061 ctgcacttgg ctcagcatca gcttgccgcg ctcgaaatga tcgagcaggc ggccgggcgt
     3121 ggcgatcagc acgtccacgc cgcgatcgag ctttttgtcc tggtcatcga aagagacgcc
     3181 accgatcagc agtgccatcg aaagcttgtt gtatttgccg tagcgctcga agctctcggc
     3241 gacctgcgcc gccaattcgc gcgtcggttc gaggatcagc gagcgcggca tgcgcgcctt
     3301 ggcgcggccc cgcgccagga tctcgatcat cggcagggtg aaggacgcgg tttttccggt
     3361 gcccgtctga gcgatgccga tcagatcgcg gtgcttgagc accagcggaa tgccgcgcgc
     3421 ctgaatcggc gtcggcttcg tgtaaccgct ttcggcgacg gccttcagta ccgaaggcga
     3481 aagccccaaa tgctcgaaac cggggaaatc cgtttcggaa ttcggaagaa tttcggaagt
     3541 tgctgctgtg gcttcttcca cgccggcttg cgcgccggca ttcgtgtctg tcgtcatgcg
     3601 ctcctgtaag gggcgcattc cgcgcccaag cagcacgccc aaatcggccg ctgcaacatc
     3661 atcggaccgc aacatagtaa gaatatttca aaagtcaagg acgtgctagc gacaggacgg
     3721 gcttgctgga cgcccttcct ggggcgagct tgctgcctgt ggccttgctg ccgcatcgcg
     3781 gtggatatag ccgtgcatcc cgatggtgaa gctcgatcac cttacgcttg ccgttgggga
     3841 ctggcgcgtg tcccgcgact ggtatgtcgg tcatctcggc ttcaaggtcg agttcgaagt
     3901 tccgcacggc ggcagttctg gattgggcgc cgtggcgatc caggacgatt cgggatttac
     3961 agtgtttctg gagcaattcg acaaaccggt tctgagcggc cagtcctcct gcacgattca
     4021 ggtcgacgaa gtggacgcgt tgttccaacg cctgaagggt tccgtcaaag taattgccga
     4081 acccgggagg cagttctggg gatatggcgc ggtgatcgcc gatgccgacc gccatctgct
     4141 gcatctctat gatacgacat caatgaacga aaaagacgga taggacacct cgccggcctt
     4201 tgcccgtgcc caaggctgtg caaaacaccg aagcacgagg gcggcagggt tgcggcatga
     4261 tcgataatcc gagccgggca atggggcagt tgaacacccc aaaaacaggc ctgttgcggc
     4321 tgtttatcac gatcgcgctg ctcgcggtcg cggcgtcgtt ctggtttctg cagatctatg
     4381 gttcggcccg gccacaagtt gcgccgctcg atgcgtcgcc gcgggcattc tccgcggcgc
     4441 gcgccgagca cgtgctcgcg cagctgttgg agccggagat tccgcatccg gtctcgagcg
     4501 acgaaaatgc caacgtacga gcgcgcatcg tgcaggaact gtctgtgctg cgcgtgccgg
     4561 ccttcgtcct tcgcagcttc gcctgccatg ccatgcacag ttacggagtg ctcacgtgcg
     4621 cgacggtgaa cgacatcctc gcggtggtga aaccggggca gggcagggcg atcgtgctgc
     4681 tcgcgcatta cgactccgtt ccggccggcc ccggcgcggc cgacgatgaa tccggcgtcg
     4741 cgaccgtcat cgagacgata cgcgcactgc tgacgcgcgg tcttaagagc cggcatccga
     4801 tcctggccgt gctgacggac ggcgaggaag caggtctcct gggcgccgcg gcgttcctgc
     4861 atgagccggc gctgaaagcg cgcgtcggcg cggtcgttaa cgtagaggca cggggcaatc
     4921 aggggccgag cctgctgttc cagaccagtc ccggcgacgg gccgctgatc gatctctacg
     4981 cgaacaacgt gcccgattac gccacaagtt cggtatatgc cgaaatctat cggttcctgc
     5041 cgaacgatac cgacctcacg ctgttcatcc gcgacggatt cccatcgttc aatttcgcct
     5101 ttgcaggaaa tatcgccgac taccacaccg cgctcgacag gcgcgtcaat ctcgatcccg
     5161 tctcgctgca ggagcagggc gacaacatgc tgggcgtggc gagtggactc gaacaggcgg
     5221 atttcgcgaa gcttcgcggc agcgacgaca tttatctcga cattctcggc cgcgtgcttc
     5281 cgcgcgtgcc tgcagcttgg gatttgccgc tttctcttgc tgcattcgta ttgctgcttg
     5341 ctgcaacact gcttgcgaga cgcgaaccga tttcctggcg gcgatggctt tgtgcgtttg
     5401 caatcacgcc cgcgcttgtt ctcgtcgcag cgggaagcgg cttcgcgctt caagccattg
     5461 ctgcctttgt ggccggcacc ccggacccgt cttatgcgca tcccgcggcg ttacgcatcg
     5521 cgcttgcgtt cggcctcgcc ggcgcgactt tgcttgtctc gcgacttacc cttccaggct
     5581 atgcaccaac cgctgtgtgg ctgtggattg cggcgctcgg catcgtggtc gcgatctttc
     5641 ttcccggcct gtcgccgtac ttcctgatcc cggcgagcgc ggcggcgatt ctcctccttg
     5701 caacggcatg cattccggga ggttgggaca gcgccgccgg tgcgatcgca gtcttcgccg
     5761 cggcgcttgt agcgcttctc gtgtggagtt cgatcagcgc gtctggcgag tcggtcatgg
     5821 ggctcaagct ccatccgcta tttaccgttc ctttcgcaat cggtcttacg acgctcgtcc
     5881 cgcttttcat gcgttacgcg atgccgcgcc ggttatgggc aactgcgacg acgacgttgt
     5941 ttgtcgccgc cctttgcggc gcagtcgtgc agggcctgga acccgcatac agctcgacgg
     6001 ccgcgcagcg cttgaatgtc acctatgtcg aggatcgcca tcgcgctctg tgggctgttg
     6061 acaccttggc gcctgttccg ggcgaaatgc gggagattgt ttcattcgcc tccgaacctc
     6121 aacgactgtc gcgcgcgatg ccgcttgctt acgtggcgcc ggctggcgcg ccacgcttcc
     6181 cgctgccgac tgcaaccatc gcgtcggata gatacgtcgg agcaggccgc agcctgactc
     6241 ttgtgctaca cggttcccgc aaggcggcac aaatgatttt ggtaattccg agatccgcga
     6301 tgctgaaagc catcgatctc agtggtcggc attttgacgc gccgccttca tgggcaaatg
     6361 aggattcggt cgctcttgcc tgcatgagcc gcgactgcgc ctccaccacc gtgaccctca
     6421 ccctggcttc acgaagcacg caagcgctcg aactgtacga acatcgcttc gggttgccgg
     6481 attttgccaa acacctggtc gccgcgcgtc cgccgaccgc ggtgcaatcg cagaacggcg
     6541 atggagttgt gctgatgaac aagattctcg tgccggcgct taggtagagc gagtcggatc
     6601 cgctatacgc aatttgattt gtcgtggccc gcgaaagaag aggttgcggg gaaccatggc
     6661 aggcacgggt ttcatgggtg gccgggacaa cggcccggcc atgcggattg gctcttggac
     6721 gttcgattca cagatcgacc tctccgacga accgggcgtt ttcctggatg aactggaagc
     6781 gcaactcggg tcgcttgccc atcagccgct ccacgagggt tgcggtttct tcgcgctcgc
     6841 catctgcgat ctccacccgc tcgagcgtgc gcatgccggg cttcatcgtc gtttccttga
     6901 gctggttggg catcatttcg ccgagcccct tgaagcgcga gacctcgatc ttcgcacccg
     6961 gcttgaacgt ggtttccagc agctgctcgc gatgggcgtc gtcccgcgca tagacgatct
     7021 tgccgccctg ggtcagcctg tagagaggag gcagggccag atgaaggtgc ccgtttgcga
     7081 tgaggccagg catttcgcgg taaaaaaaag tcagcaggag cgaggcgatg tgtgcgccgt
     7141 ctacgtcggc atcggtcatg atgatcacgc gctcgtagcg cagttcctcg tcgcgatagc
     7201 gcgcgcccgt gccgcagccc agtgcctgca ccagattggc gagttcctgg ttctgctcga
     7261 gcttcccggt tgccgcgctg gcgacattga ggattttgcc acgcaggggc aacacggcct
     7321 gagtggcgcg gtcgcgcgcc tgcttcgcgg agccgccggc agagtcgcct tcgacgagga
     7381 agatttcggt cccgctcgca ttgtcgcggc tgcaatccgc gagtttaccc ggtaagcgca
     7441 atttccgtgt cgccgtcttg cgcgagacct cgcgttcctg cttccggcgg agccgttctt
     7501 ccgcttggtc gacgatgaag gcgaggagtt tgtcggcttc gggcggatgt tcggcgagcc
     7561 agtggtcgaa atgatcgcgc accacattct cgaccagacg ctgcgcgtcg ggcgtcgaca
     7621 gtttctcttt ggtctggccc tggaattcgg gctcgcggat gaagatggaa agtaccgcgc
     7681 acgccgtttc catcacatcg tcgggtgcga tgagcgcggc cttgcggttg ttcgcccgtt
     7741 cgccataggc ccgcaacgct ttggtcaacg cgttgcggaa accctgctcg tgtgtgccgc
     7801 cttgcgtagt gggaatggtg ttgcagtagg agtggataaa gggatcggcc tccgggaccc
     7861 aggagaccgc ccattcgacg gcgccttttc cgtcgcggtt ctcgctgcgg ccgtaaaaat
     7921 tccgcgaggt gacggcagcg cggccttcga tctccgaatt gaggaaatca acaatgccgc
     7981 cgggaaattt gagcgtctct tcggctggcg tgcctccatc gcccttcagg agctctggcg
     8041 cacagctcca gcgaatctcg acgccgcgga agagataggc cttcgaacgc gccatgcgat
     8101 aaagcgcccg gggcgaaaaa tggacagact tgccgaagat ctgctcgtcg ggtttgaacg
     8161 acaccacggt gccgcggcgg ttgaccgcgc ccgcatcctt cagttttccc accgcgtgac
     8221 cgcgcgcaaa gcgcatgcga aaggcgtggc gctcgcggca gacctcgact tccatccatt
     8281 ccgacagcgc attcaccacc gacgcgccga cgccgtgaag tccacccgag gtgtcgtaga
     8341 ccttgccccc gaactttccg ccggcgtgca gcgtggtcat gatcacttcg agcgcggatt
     8401 tgttcttgaa tttgggatgc ggatcgacgg gaatgccgcg gccattgtcg cgcatggtga
     8461 gcacgtcgtc gggtttgagg tcgatctcga tgcgcgtcgc atggcctgcg accgcctcgt
     8521 ccatggcgtt gtcgagaatc tcggcggcca ggtggtgcag cgcatgctcg tctgtcccgc
     8581 cgatatacat gcccggccgg cgccgcaccg gctcgagacc ttccagcacc tcgatatcct
     8641 tggccgtgta ggctttggac ggagcaggtg cgccctcaaa cagatcggga gatttcgaca
     8701 ttcaggatcg cgtgcgaatc gtcgatgtca cgttagacga cagccgcatc acccgcaccc
     8761 tgcgcttttt ttttgcgcag aggacgcgga ggatgcacgg tggacacggg ggaagaagaa
     8821 ccgcgcgcct tcggcgcgca actattctac tgcgcatcct ccgcgtcctc tgcgttaaaa
     8881 cttcctcctt acacgctgta gtacattttg aattcgatcg ggtggggcgt gtgttcgaag
     8941 gcgtggactt cgcccatctt gagctcgatg taggagtcga tgaaatcgtc ggagaacacg
     9001 ccgcccttct tgaggaaggc gcggccggca tcgacgcatt cgagcgcctg gcggagagaa
     9061 ccacacactg tcggcacgtc tctgagctct tccggcggca gcgcatagag atccttgtcc
     9121 atcggatcgc ctggatgaat cttgttctcg atgccgtcga gccccgccat cagcatggcc
     9181 gcataggcga gatagggatt tgcgccggga tcgggaaacc gcacttcgat ccgttttcca
     9241 ttggggcttg cggaaaaagg aatccggcat gatgccgagc ggttgcgcga cgaataagcg
     9301 agcagcaccg gtgcttcgaa accgggaatg agtcgcttgt aggagttggt aagcgggttg
     9361 gtgaacgcgt tcacggcctt cgcatgccgg atgatcccgc cgatgtaaaa gagacacgtg
     9421 tcggaaagat ccgcgtagcc ggagcctgca aacagcggct tgcccccctt ccagatcgac
     9481 tggtggacat gcatgcccga gccattgtcg ccgtagacgg gcttgggcat gaaggtggcc
     9541 gtcttgccgt aagctgcggc aacctggtgc accacgtatt tgaagatttg catcgcgtcg
     9601 ccgcagcgcg tgagcgtttt gaatttgaag cccagctcgt gctgcgcgca ggcgacttca
     9661 tggtgatgtt tctcgggctc gatgccgaga tcgcccatca cggcgagcat ttcgctacgg
     9721 atgtcctggg cggaatcgac gggcggtacg gggaaatagc cccccttcac ggcgggacgg
     9781 tgcccaaggt tgccgccttc gaactccgtg ccggaattgt aggcgccttc ggcagagtcg
     9841 atatagtaga agcccttgtt catggtgctg tcaaagcgaa cgtcgtcgaa gataaagaat
     9901 tccgcctcgg ggccgaaata ggccgtgtcg ccgacgcccg acgacgccat gaatttttct
     9961 gctgccttcg cgatcgagcg cgggcaccgc gcatagcgct ggccggttgt cggctcaatg
    10021 atgtcacaga acatcgcaag cgtggtctgc gcgaagaagg gatcgatgat tgcggtgtcg
    10081 caatcgggaa gcagcgtcat gtcggattca ttgatcgcct tccagccggc gatcgacgag
    10141 ccgtcgaaca tcgtcccgtt ggtcaggaaa tcgtcgtcga ccagtgacag gtcgtaagtg
    10201 acgtgctgcc acttgccgcg cggatcggtg aagcggaggt cgacgaattt gacctccttc
    10261 tccttgatga gtttcagcac tgaacttgcg ttggccatga ctttccctcg acgttgcagc
    10321 gggaatcccg cagttaattc tcagattgcg tccgcgccgg tctctccggt gcgaatgcgg
    10381 atggcttgct cgatgtcgaa cacgaatatc ttgccgtcgc cgatgcggcc ggtgcgcgcg
    10441 gctttctgaa ttgcttcgag tgctgcttcg agccgatcgg cagggaccac cacctcgatt
    10501 ttcacctttg gcaggaaatc gacgacgtat tcggcgccgc gataaagctc ggtgtggccc
    10561 ttctgacggc cgaaaccctt ggcttcgaca acggtgattc cctgcacgcc gatttcctgc
    10621 agggcttcct tcacctcgtc cagcttgaac ggcttgatga tcgcttcgat cttcttcatg
    10681 gccgactccg ccgtatcgac aggctccctt aaccaacaag cagtcttcgt gccagacgaa
    10741 acggggttat ccacatcgga ttcaaaagct tgccggaacg tcaaatgaca tttgcacaag
    10801 aaatttgcgg tcagcctctt ttgcgggcag caccgcccgt gtgggctcac ccttcgacag
    10861 actcagggtg aggggccttc cctcatgctg agcctgtcga agcaagagcc tgccgaaaca
    10921 tgagcttgtc gaagcaggac ggcatgccta aagggttcgg gatgagcggg atgcgattca
    10981 atccggtatt ttctggcctt ccgacaacga tttttacggt catgtccgcg ctcgcggtcg
    11041 agcacaaagc catcaatctg gggcagggct ttccggacga agacggcccg ctcgcgatcc
    11101 gcgaagcggc ggcggcggct ttgcgcgaag gccccaatca atatccgccg acacgcggac
    11161 ttccggcgct tcgtggggcc attgccgccc acgcgaagcg cttttatggc ttcgattttg
    11221 atccggagcg caacatactg gtcacctcgg gtggaaccga ggcgctgacg gcgagcattc
    11281 tggcgttggc gggtgagggc ggcgaggtgg ttcttttcga gccggcctat gattcgtatc
    11341 gtcccatcgc gctagcggca ggagccctgg tgcgcagcgt caagctcgcg ccgccgggct
    11401 ggcggatcga ggagcaggcg ctcgccgcgg ccattacgtc gaaaacgcga attgtcatgc
    11461 tgaacacgcc gctcaatccg atcgggcgcg tcttcgacgg ggacgagctg gccatggtgg
    11521 cgcgagtgct cgcgcacagc cacgcggtcg taatctgcga cgaagtctac gagcacctgg
    11581 tgttcgacgg gcgcccccac ctgcccctga tcggttttcc aggcatggaa gagcgcgtcg
    11641 tccgcgtcgg ttcggccggc aagatgttct cgcttacggg ctggaaagtt ggttgggtga
    11701 ccggagctgc gccgctgatt gatgtgatcg ccaaagcgca ccagttcata acattcacca
    11761 cgccgcctgc gcttcagcgc ggcacaactt atgcactttc caacgaaatg gacttcacgc
    11821 tcatgcttac gcggacgctg caggacaatc gcgacctcct tgcgggccgc ctcgcgcgca
    11881 tgggattcga cgtcctgccc tgcgagggaa cctattttct gaccgccgga atcggcgccc
    11941 tcaccaatga gcgcgacgat gatttctgcc gacggttggt gcgcgaggcg ggcgtagcgc
    12001 tgatcccatt gagcgcattc ttcgaaacgg gcgaacccgg ccatctggtg cggttcgcct
    12061 tctgcaagaa gcgcgcgctg ctcgaagaag cgatgaatag gcttgagcga cactttgcgg
    12121 ggcaggagcg agttcgatga gcaggtgtct cgggcgtgct ccattttctt tccccacacg
    12181 tgggggtgga aaaccgtgag tcaggaaatt ctcactgtcg ccgagatgta tgccgcggac
    12241 cgatatgcgg gcgagaatgg cgtgccgtcg ctgacactca tggagaacgc ggggcgcgcg
    12301 atagcgatgg agattacgcg acgctggtcg gcccggccga cattcgtgct ttgtggaccg
    12361 ggtaacaatg gcggcgacgg ctttgtcgtc gcgcgccatc tggccgagcg cggctgggag
    12421 gcacgcgtgg cgttgctggg cgatcgcgac cgtctcatgg gcgacgctgc tgccatggct
    12481 acgcgctggg cgggcgaggt attgccgttg tctcacgatc tcgtgagcga tgcctctctc
    12541 gtcgtggatg cgatgttcgg gaccgggctc gcgcggccac tcgagggagt cgtgggcgag
    12601 accgccctgg cgctcaacgc gtcagatgcc gtagttgtgg ccgtcgatgt gccgagcggc
    12661 gtgcagggcg atctggcacg cccgatcgac ggccccaagg gagtagcggt cgaagcggac
    12721 ctgaccgtca ccttcttccg caagaagcct gcgcacgtgc tgatgccggg acggctgcgc
    12781 tgcggagagg tcacggtcgc cgacatcggc attccgccgg cggcgcttga aactatccgt
    12841 ccacggatgt tcgagaacgc ccccgcactg tggggcacgg gctatccatg gccgcaaccg
    12901 ctcgcgcaca aatatgcgcg cggacacgcg ctcgtcgtta gcgggcctgc gcatgcgacg
    12961 ggcgccgccc ggcttgcggc gcgtgcggcg ctgcgtgcag gcgccggcct tgttagtgtg
    13021 gcaagtccgc tcgatgccat tgcggtcaac gccgcttcac tcaccgctgt gatggtcaag
    13081 ccgttctcag gcgtcgcagg tctcgcagac cttctgaagg acgcgcgctt caacgccatc
    13141 gtcatcggac cgggatgcgg cgttgggcgc acgacgcagg aaatggttgc ggccgttctg
    13201 gcgagcaacg cggccgtcgc cctcgatgcc gatgcgctta cctcctttgc cggcgagccg
    13261 gacacgctgt tccgccaatt gcgcgatccg gcggtgctga caccacacgc cggagaattc
    13321 gcgcgtgtct ttccggacgt gctgaagcgc actccgacgc gcatcgaagc ggcgcgagaa
    13381 gcggccgcgg tcgcgcggtg cacggtgctg ctcaagggcc ccgataccgt gattgcggcg
    13441 gcggatgggc gaatcgccgt cacgacaaat gcgccgccga cgcttgcaac ggcaggcgcc
    13501 ggcgatgtgc tttcaggcat tattaccggc ttgctggcac agggaatgga ctccttctct
    13561 gcggccgccg ccggcgcgtg gctgcacggc gaggccgcgt cgcgctccgg ttacggactg
    13621 atcgcggagg atttggcgga gattctgccg gcagttctct cctctctgcg ggacacaccg
    13681 gcgtgagcac accccaagtg cggcgcgccc tcgcttcgga catgtccgca cttctcggca
    13741 tctacaatca ctatgtcttt catacccaca tcaccttcga catcgagccg cgcacactcg
    13801 aacagcggct cgaatggttt gccgggtttt cgtcagccgg ccgccatcaa tgcttcgtcg
    13861 cggtagaggg cggcatgccc atcggatggg catcgtcgag cccgttcaag gaacgcgcgg
    13921 cctacgacac ttccgtcgag acaagtgttt atctggcgcc ggatgcgacc ggcagaggca
    13981 tcgggcggaa gctctacgaa acccttttcg aggcactgca acacgcagat gcgcatcgcg
    14041 cctatggcgg aattgcccag cccaatgaag cgtcggtcag gcttcacgaa aggatgggat
    14101 ttagctgcat gggcacctac cacgaggtgg gccgcaaatt cggccgcttc tgggacgttg
    14161 ccgtgtatga acggcctctg ggtcgagaac ccctagtcac cccctgacgc gcctgataat
    14221 aggggcgcgc ttcggcggtt ggcgccgccg gaatgcccaa gcgcgggcgt ggtggaatcg
    14281 gcagacacgc gagatttagg ttctcgtgac gaaagtcgtg ggggttcaag tccctccgcc
    14341 cgcaccagcg ccgaggccgg gcacagcgca gggatttgtc caaatgcaga tcacagaaac
    14401 cgtttccgag ggcctgcgcc gcgagttcaa ggtggtgatc ggcgcgggcg accttgatgc
    14461 gcgcctcacg ggccgcctcg aggagatgaa gtcgcgcgtt catctcaagg gttttcggcc
    14521 tggaaaagcg ccggtttcct atctgaaaaa gacgtatggc aaatcgatga tgggggagat
    14581 cgtcgaacag gcggtatcgg aatcgagcca gaagacagga gaggaccacg cattgaagct
    14641 tgcgtttccg ccgcaggtcg atctggtaag cccgatcgaa gaagttgtcg gcggcaagtc
    14701 cgatc
//
LOCUS       DASZMC010000273         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1239220,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000273 DASZMC010000000
VERSION     DASZMC010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239220"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..1083
                     /locus_tag="VG274_05575"
     CDS             493..1083
                     /locus_tag="VG274_05575"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWE06156.1"
                     /translation="MRRRLLADPPELPLAPRRSDYDLNPRAQRPRLRELAPAAVLFPI
                     VRRAAPTVLFTRRTAHLARHAGQVSFPGGRLDENDPSLVDTALRETREETGIGPSFIS
                     VAGFLDAYETGTGFAILPVVGLLAEGFSLVPDPHEVAEIFEVPLAFLLDAANCERQIG
                     EWQGATREFYSFRYDRHHIWGATAAIVVNLRERLCQ"
     gene            1080..1346
                     /locus_tag="VG274_05580"
     CDS             1080..1346
                     /locus_tag="VG274_05580"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HWE06157.1"
                     /translation="MIRLILTRTLLFALPFALYGAWLLLMRLRAKTPGPHTPWTVLIV
                     AGLALVAASFIYVGLTEGESTSGVYVPPHVANGKIVPGHVEKVR"
     gene            1343..2590
                     /locus_tag="VG274_05585"
     CDS             1343..2590
                     /locus_tag="VG274_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HWE06158.1"
                     /translation="MTRRLDPSRDSWMRAGETRAVMGALLASGGEARFVGGAVRNALL
                     NREIGDIDIATPLLPAEVTRRLESAGITALPTGIEHGTITAITGGKAFEVTTLRRDVS
                     TDGRRATVEFSNDWAEDAGRRDFTINALYASLDGEVFDFTGGLADLEAGHVRFIGEAS
                     ARIREDYLRILRLFRFHAWYGKGEIDAESLRAAAAERSGLQKLSGERIQKEVLRLLEA
                     ENPVPSLRAMAATGILAEIVPDGARLDRLERLTAIDVANFFAADPDLRLAALLPDDPA
                     AAKALAERLRLSNENRERIEDLAGAGEKLAPYLSVREVRRLLYRLGARRLRDRVLLRW
                     SEDGKDSNTIAWRALLALADAWTRPRFPLTGRDVMAAGVPEGPRVGCVLAEVEAWWID
                     SDFTEDRLSLAERLKAIVQAGTF"
     gene            complement(2595..3245)
                     /locus_tag="VG274_05590"
     CDS             complement(2595..3245)
                     /locus_tag="VG274_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HWE06159.1"
                     /translation="MPTLHILPPALAALLQVILIDITLAGDNAVVIGMAAARVPAAER
                     RRVMFWGLVAAVLLRLAVAYVVLYFLLHTSFFLVLAGGFLLLWVAWRLYREIRLGAED
                     AVGMTTISDLRFGDVQAGLPARTPDVRRAIWQIAIADLSMSLDNVLAVAGAAMNHVWV
                     LAVGLLLSIALMGLAASLIAKLLQKMPWISYAGLIIVLYVALRMIWFGGMEIYYAT"
     gene            complement(3266..4132)
                     /gene="hemF"
                     /locus_tag="VG274_05595"
     CDS             complement(3266..4132)
                     /gene="hemF"
                     /locus_tag="VG274_05595"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HWE06160.1"
                     /translation="MSPAALEQHKAKASAWFEHLRDSICETCEAIEKEYTGPPRELSV
                     GRFEQQSWTRAGDAADGGGGVISIMRGRVFEKIGVNVSTVHGRLSPEFAREIEGAEND
                     PRFWASGISLVAHPRNPHVPTVHLNTRFLVTTRAWFGGGTDLTPMLPAEEDARDFHAA
                     LKHVCDRHHPDYHARYSKWCDEYFFLPHRGEHRGAGGIFFDQLASGDWGGDFAFTRDV
                     GLAFLDIYPQIVRRRMNEPWTEAQRAQLLVRRGRYVEFNLLHDRGTQFGLKTGGNVEA
                     ILMSLPPEARWP"
     gene            complement(4129..>4420)
                     /locus_tag="VG274_05600"
     CDS             complement(4129..>4420)
                     /locus_tag="VG274_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HWE06161.1"
                     /translation="AEIVRHASWQAYHTNLSGRLIVLTTRAQRSYTAFMFGKDDTLML
                     GRESAGVPDYVHETAHARLRVPMQSGQRALNVAQAGAMVLGEALRQTNSFPQ"
BASE COUNT          777 a         1421 c         1420 g          802 t
ORIGIN      
        1 ggagcgtgac aagcgtctga tcgatgacct cgcgctgacc gaaaatgacg cgtgccgcgg
       61 cagcgcgcac cttgccgaaa agctccgcgt acatttccgc ctgcgccacg ggcgaatcgt
      121 cggtcgtaag tgtgtcgtcg atcgatcttg tgtcgttcat ctgctgcaat atcttgtctc
      181 aggcgctgcg ggcgccgcca tgttccgccg ctgcttcaat tgcggccggc cgcgccgctt
      241 cctatcctcg gacacggcct ccaggagggc gtttatgttg cattccggct ttccccgcgc
      301 aaccgcgctc tgccccatcc agggcccgga atccgcacaa cgccgtgagt gcgcggcgcc
      361 gcgaatgcaa ccgttttggg aagcggttgc ttgggcgcaa gcgccggcgc gtacggattg
      421 tccttgacgg agtgcattca gccacggccg tccccgctcg tgcagcgtat tgccgaggag
      481 gggcttcaac ctttgcgccg gaggcttctc gcggatccgc ccgagctgcc gctcgcgcca
      541 cggcgcagcg actatgatct caatcctcgc gcgcagcggc cacggctgcg cgagcttgcg
      601 cccgcggcgg tgctgtttcc catcgtcagg cgagccgcgc cgaccgtgct ttttacgcgc
      661 cgcaccgcgc atctcgcacg gcacgcgggc caggtgagct ttcccggcgg acgactggat
      721 gagaacgatc cctccctcgt cgatacggca ttgcgcgaga ccagagagga aaccggaatc
      781 gggccaagct tcatttcggt tgccggtttt ctcgacgcct atgagacggg aaccggcttt
      841 gcgatcctgc cggtggtcgg ccttctcgcc gagggatttt cgctggtacc cgacccgcac
      901 gaagtcgcgg aaatcttcga agtcccgctt gcgttcctgc tggacgccgc gaattgcgag
      961 cgccagattg gcgagtggca gggcgcaaca cgcgaattct attctttccg ctatgaccgc
     1021 catcacatct ggggcgccac cgccgccatc gtggtgaatc tgcgggagag gctttgccaa
     1081 tgatccgcct gattctgacg cggacgctgc tttttgcact gcccttcgcg ctttatgggg
     1141 cgtggcttct gctgatgcgg ctgcgcgcga agacgccggg gccgcacacg ccatggacgg
     1201 ttctgatcgt cgcgggcctc gctctggttg cggcaagttt catttatgtc ggcctgaccg
     1261 agggcgaatc gaccagcggc gtgtacgtgc cgccgcatgt cgcgaatggc aagatcgtgc
     1321 cgggccatgt cgagaaggtg cgatgacgag acggcttgac ccgtcccgcg acagctggat
     1381 gcgcgccggc gagacgcgcg cggtgatggg tgctctcctg gcatcgggcg gcgaggcgcg
     1441 attcgtgggc ggcgcggtgc gcaacgccct gctgaatcgc gagatcggcg atatcgacat
     1501 cgcgacgccg cttcttcccg ccgaagtgac gcgccgcctg gagagcgcag gaataaccgc
     1561 gctgccgacc ggcatcgagc acggcaccat taccgcgatc accggcggca aggccttcga
     1621 ggtgacgacg ctgcgccgcg acgtgagcac ggatggacgg cgcgcgacgg tcgaattttc
     1681 gaacgattgg gccgaagacg caggccgtcg cgatttcacc atcaacgcgc tctatgcctc
     1741 gctcgacggg gaggtgttcg atttcaccgg cggtctcgcc gatctcgagg cgggccacgt
     1801 gcgcttcatc ggcgaagcgt cggcgcgaat ccgcgaggac tatctgcgga ttctgaggct
     1861 cttccgcttc cacgcctggt acggcaaggg cgagatcgat gcggagagtc tgcgcgccgc
     1921 ggccgccgaa cgcagcggat tgcaaaagct ttccggcgaa cgcatccaga aggaagtgct
     1981 gcgcctgctc gaagcagaaa atccggttcc ttcacttcgc gcgatggcgg cgaccggaat
     2041 tcttgccgag atcgtcccgg acggcgcacg gctcgatcgc ctcgagcggc tgactgcgat
     2101 cgacgtggcg aatttcttcg cagccgatcc tgacctgcga ctcgccgcgc ttctacccga
     2161 cgatccggct gccgccaaag cgctcgccga gcgtctgcga ctctcgaacg aaaaccgcga
     2221 acgcatcgag gatctggccg gcgcaggcga aaaactcgca ccctatctgt cggtgcgcga
     2281 ggttcgccgc ttgctctatc ggctgggcgc ccgacgtctc agggaccgcg tgcttctgcg
     2341 ctggtcggaa gacggcaagg attccaacac gatcgcctgg cgcgcccttc tcgcgctcgc
     2401 cgacgcctgg acacgccccc gctttccgct cactggccgc gacgtgatgg ctgccggcgt
     2461 tcccgaaggg ccgcgcgtcg ggtgcgtgct cgccgaagtc gaggcatggt ggatcgacag
     2521 tgacttcacc gaagaccgcc tctccctcgc cgaacgcctg aaggccatcg tgcaggcggg
     2581 tacgttttga cgcgctacgt cgcgtaataa atttccatgc cgccgaacca gatcatgcgc
     2641 agggcgacgt agagaacgat gatgagtccc gcatagctga tccacggcat cttctgcaga
     2701 agttttgcga tcagcgacgc ggcaagcccc atcagcgcga tcgacagcag aagtccgacg
     2761 gcgagcaccc agacgtgatt catggcggcg ccggctaccg cgagtacatt gtcgagcgac
     2821 atggaaagat ctgcaatcgc gatttgccag atcgcgcggc gaacatcggg agtgcgcgcg
     2881 ggcagccccg cctgcacgtc gccgaacctc aagtccgaaa ttgttgtcat cccgacggcg
     2941 tcttcggcgc ccaggcgaat ttcccgataa agccgccatg ccacccagag cagaaggaat
     3001 ccgccggcga ggacgagaaa gaaggacgtg tggaggagaa aatacagaac tacgtaggcc
     3061 acggcgagac ggagaagcac ggccgccaca agcccccaga acatcacgcg ccgccgctcg
     3121 gctgcgggaa cgcgtgccgc ggccatgccg atgacaactg cattgtctcc tgccagcgtg
     3181 atgtcgatga ggatgacctg caaaagcgcg gccagggcgg gaggcagaat atgcagtgtg
     3241 ggcacgtgtc gtgcgggcgg gcccttcagg gccagcgcgc ctccggcggc aacgacatca
     3301 ggatggcttc cacatttccc cccgttttca gcccgaactg tgtgccgcga tcatgcagca
     3361 gattgaactc cacgtaacgt ccccggcgca ccagcagctg cgcccgttgg gcctcggtcc
     3421 acggctcgtt catgcgcctt cgcacgatct ggggatagat atccagaaat gcgaggccga
     3481 cgtccctggt gaaggcgaag tcgccccccc agtcgccgct tgccagctgg tcgaaaaaga
     3541 tcccgcccgc ccctctgtgc tcaccgcgat gcggcaggaa gaaatattcg tcgcaccatt
     3601 tgctgtagcg cgcatggtag tccgggtggt gccggtcgca gacgtgtttg agcgcggcat
     3661 ggaagtcgcg cgcgtcttcc tcggccggca acatcggagt gagatcggtg ccgccgccga
     3721 accaggcccg ggtcgtgacc aggaacctgg tgttcaggtg aaccgtggga acgtgcgggt
     3781 tgcgggggtg ggcgacaagc gaaatgcctg acgcccagaa gcgcgggtca ttttcggcgc
     3841 cttcgatttc gcgcgcgaat tcgggcgaaa ggcgcccgtg aacggtcgaa acattgacgc
     3901 caattttttc gaacacgcgc ccacgcatga tcgaaataac gcccccacca ccatctgcgg
     3961 catcgccggc gcgcgtccag gattgctgtt cgaagcgccc tacggaaagt tcgcgcggcg
     4021 gaccggtgta ttctttttcg atcgcttcgc aggtttcgca aatactgtcc cggagatgct
     4081 cgaaccaggc gctcgccttc gctttgtgtt gttcgagcgc cgcagggctc attgcgggaa
     4141 gctgttcgtc tggcgcagcg cttctcccag caccatggcg ccggcctggg caacgttgag
     4201 tgcgcgctga ccggattgca tgggaacgcg aagtcgcgca tgcgcggttt catgcacgta
     4261 gtcgggcacg ccggcgctct cccggcccaa catcagcgta tcgtccttcc cgaacatgaa
     4321 ggcggtatag gagcgttgcg ccctggtggt caaaacaatg agccgccctg aaaggttcgt
     4381 gtgataagct tgccatgaag cgtgccggac tatctcggcg
//
LOCUS       DASZMC010000274         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4785091,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000274 DASZMC010000000
VERSION     DASZMC010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4785091"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(838..>1591)
                     /locus_tag="VG274_05605"
     CDS             complement(838..>1591)
                     /locus_tag="VG274_05605"
                     /inference="COORDINATES: protein motif:HMM:NF015457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06162.1"
                     /translation="FIFGSGTSFYRQIFPDGALSLGFSATAMHWLSARPCMIADHVQA
                     VGAAPAERERFRAQSLNDWETILLHRARELRAGGRLVFANFCVDEAGRYLGGTRGANM
                     FDEFARHWRELRAAGRITEAEYVDATFQQFYKTRQEFAAPFRDATSPVSKAGLRLEHI
                     STIVTPCPYAEEFGVHRDAAAFAKAYMPTLRSWSETVFAGALDPTRPPEERAAILDEF
                     YGAYEAEAAREPERHRMDYVHCVMEISKAGVG"
BASE COUNT          306 a          492 c          532 g          261 t
ORIGIN      
        1 gatcgcggct gagggctcga gcgcgtcgaa tgacgagtga gtgccggcca tggtgtcgaa
       61 tcgggcgaag cgggaagccg ggcgcggcgc aagccgggaa gcgcgggggc cgaacgggga
      121 tcacgtcgta acgctcgcga cgaccgcggt cggaatattt cttgcttatt tctttctgga
      181 aaaacgaacc tgtggtacgg tcttcggata ggcccgcatc ctgtccgcgg gtcgccggag
      241 ccggaacatc atcacaggcg tggaacgtga catttcccag tccgaccgcg agcgcgcgtg
      301 cgcgcgctcg ctcagaagcg gtgggccgcg aaggcggcac ggcgcgaaac caaggcgacg
      361 gaccagctcc ggctcgatgc acccgatcgc tgcatcagcc ccaaccctcg agacggcgcc
      421 gggcgcggcg acgatgcgct ggagcgtgcc gatctcagga tccgttgctg gagcaggtcg
      481 gcgcgatcga gcgccatcac ggaccgacgg ttggggcgca tcgcgatatc gaccgttgca
      541 attgtcgtcg atcgagcgag gcggaaaatt tttctgcgcg caaagccttg aaaatactca
      601 gaaccggaaa atccacgagg cacggggcgc agtcgacgat gcgctggagc gtgccgatct
      661 cgacaccgcg gatccgccgc tcgaagggtc ggcgcgatcc agcgacatca gggaccaacg
      721 gttgcggcgt cgtcgccata tcgaccgtcg caattatcgt ccctcgagcg aggcggaaat
      781 tttcctggcc gcaaagcctt gaaaatccac aaaaccgcgc aaaaatctcg atttgactca
      841 ccccactccg gccttcgaga tttccatcac gcaatggaca tagtccatgc gatggcgttc
      901 gggttcgcgc gccgcttcgg cttcataggc gccgtagaat tcatcgagga tcgcggcccg
      961 ctcctctggc ggacgggtgg ggtcgagcgc gccggcgaag accgtttccg accaggagcg
     1021 caaggttggc atataggctt tggcgaacgc tgcggcgtcg cgatgaacgc cgaattcctc
     1081 ggcgtagggg caaggcgtca cgatggtcga aatatgctcc agccgcagtc ctgccttgga
     1141 gacgggggaa gtcgcgtcgc ggaagggcgc ggcgaattct tgcctggtct tgtagaactg
     1201 ctggaacgtc gcatcgacat attcggcttc cgtgatccgc ccggccgcgc gcagctcgcg
     1261 ccaatggcgg gcgaactcgt cgaacatgtt tgcgccgcgc gtgccaccca gatagcggcc
     1321 ggcttcgtcg acgcagaaat tggcgaacac cagccggccg cccgcacgca attcgcgcgc
     1381 gcgatgcagc aggatcgtct cccaatcgtt caggctctgg gcgcggaatc gttcgcgctc
     1441 ggcgggcgcg gcgccgacgg cctggacgtg atccgctatc atgcacggcc gcgcgctcag
     1501 ccaatgcatc gcggtggcgg agaagcccag cgacagcgcg ccatcgggaa agatctgccg
     1561 atagaaactc gtgccgctgc cgaagatgaa g
//
LOCUS       DASZMC010000275         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2317450,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000275 DASZMC010000000
VERSION     DASZMC010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317450"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VG274_05610"
     CDS             complement(<1..344)
                     /locus_tag="VG274_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06163.1"
                     /translation="MAAAPDFGGSLESFRAFPHSGTMAGEQVGPRGMDLPPLPEAAQR
                     AFFDQVLAQALAAEQVAGIFEIDIALAGTTIRLSFAGAGMRDALLPALAHLQVERRGA
                     TDAVFHVWESEA"
     gene            393..1418
                     /locus_tag="VG274_05615"
     CDS             393..1418
                     /locus_tag="VG274_05615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWE06164.1"
                     /translation="MVSISVAMATYNGARFLRAQLESLAAQTHPPFELQVGDDGSSDR
                     TLEILEDFKRTAPFPIVVHRNASKLDITENFLRTAGRCTGEWIAFCDQDDVWLPRKLA
                     RCARGIERGPADLKLVAHNATIIFEGESRRYLKTFWRGADYRPRLGLPPHWSCAGFTQ
                     VFRRDLLTILPDPAARALRAKGVRDGHDAWIPTLAAATGSILLIGEALALYRRHGNNV
                     TIAVEQEPRSRPRKIWRALRANGQAHSDEADVASRIAMLLDEASAAAVDARSRAQLTD
                     AAMRLRAFSGANARRARLYSDRNPLRRIGVLKQLLLDGAYRSSPAPSFGPRAFVKDLA
                     YALFRPN"
     gene            complement(1394..2491)
                     /locus_tag="VG274_05620"
     CDS             complement(1394..2491)
                     /locus_tag="VG274_05620"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWE06165.1"
                     /translation="MPARRDALLPSLAQELLLRAALARDGEEACALFEQWLAVEPFDG
                     PHDEGSFRLLPLVWKRLSQASVPHPLLPRLKGVFRSNWVKMTRRIQGAAAILAALEAE
                     GIGTMIGKGLPLALAYYPAPALRPMSDVDIIVPFEDAPRASALLERDGFSVRRQSWAT
                     ERRLHHALMHVRADGTEVDLHWHVLYDCPNGAADSYFWSRAVPIDVGSARSLRPAASD
                     LLLQTIVHGLRSNPSPPFRWVADAAMILGSNEAIDWAAIEAFAEEQGLTKRLQIGLAY
                     LRAFALPIPPALIERLAARSSWLEPFELAAMRRELRSPAWRHARRAAYLLRLLKWDPH
                     RGTLLLALMQALQARRTSAAGLQVQLGRNSA"
     gene            complement(2475..3173)
                     /locus_tag="VG274_05625"
     CDS             complement(2475..3173)
                     /locus_tag="VG274_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWE06166.1"
                     /translation="MGTLSLILAVRNGANYLGEALASAVMQSRRPDEIIVVDDGSTDA
                     TGTIARGFGDIVCLRQEPRGLAAAQNVGISRAKGTFICFLDHDDLLPPDSIALRLALL
                     SKRDELAYVYGTVEQFISPDLGPEQKSRLPARLQTLTGRMTGASLFRRSAFERVGAFA
                     STLRMGYMMEWVSRAEAAGMNAASIQDVVLRRRVHATNSVHDTQTLETEYLRALRMAL
                     RHKRADRRADARPA"
     gene            complement(3197..3703)
                     /locus_tag="VG274_05630"
     CDS             complement(3197..3703)
                     /locus_tag="VG274_05630"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HWE06167.1"
                     /translation="MGRLACADSSPSLPIGPMGAENSIEQAFEVNAPDVAGEIVDGEA
                     IIMHLKRGYYYSLEGSGALIWGGVENRMKASAIADALASHFGLEAAHARQTVGALFEE
                     LLSHELVRAAAPPGQDADLSAIPLPACLYAEPRLEIFTDMQDLLLLDPIHDVDEAGWP
                     VAPRQARV"
     gene            3747..>4088
                     /locus_tag="VG274_05635"
     CDS             3747..>4088
                     /locus_tag="VG274_05635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWE06168.1"
                     /translation="MNEGAAIVETSVIIAVRNGERFLGEAIRSVLAQVSATDELIVVD
                     DGSTDRSAQIAASTADPRVQLIQQDRRGVSAARNRGVSAANGCFLAFLDHDDLWPSER
                     HGILIEALYQNR"
BASE COUNT          734 a         1365 c         1316 g          673 t
ORIGIN      
        1 ctcgcttcgc tttcccagac atggaagacc gcatccgttg cgccgcggcg ctccacctgc
       61 agatgcgcga gtgcgggtaa cagcgcgtcg cgcatgccgg ccccggcaaa cgacaggcga
      121 atcgtggtgc ccgcgagcgc gatgtcgatc tcaaagattc cggccacctg ctccgccgcg
      181 agcgcttggg ccagaacctg gtcgaagaat gcgcgctgcg ccgcctccgg gagcggcggc
      241 agatccatac cgcgaggacc aacctgctca cctgccatcg tgccgctgtg cggaaacgcg
      301 cgaaagcttt caagactgcc gccgaaatca ggagctgccg ccacggtcca gcagttgcgg
      361 cgccgccacg cgcgtgcgag gtgcgtcgac gcatggtttc gatttccgtg gcgatggcaa
      421 cctataacgg cgcccgcttc ctccgcgcgc agctggaaag ccttgccgcc cagacacatc
      481 cgccattcga gctgcaggtt ggggacgacg gctcctccga ccggacgctt gagatacttg
      541 aggatttcaa acgaacggca ccgtttccaa ttgtcgtcca tcgcaatgct tcgaagctcg
      601 atatcaccga aaatttcctg aggacggccg ggcgttgcac gggcgaatgg atcgcatttt
      661 gcgatcagga cgatgtgtgg ctgccgcgaa agctggcgcg ctgcgcgaga ggaatcgaac
      721 gcggcccagc cgatctcaaa ctggttgccc acaatgcgac catcattttc gagggcgaat
      781 cgcgacgcta tctgaagacg ttctggcgag gtgccgacta caggccgcgg ctgggcttgc
      841 cgccgcactg gagctgcgcc ggtttcaccc aggtcttccg ccgcgacctg ctgacaatcc
      901 ttcccgatcc ggcggcgcgc gcgcttcggg caaagggggt gcgggacggg cacgatgcct
      961 ggattccgac gcttgcggcg gccacaggct cgatcctgtt gatcggcgag gcgctggcgc
     1021 tctaccgcag gcacgggaac aatgtgacga tcgcggtcga gcaggagccg cgcagccggc
     1081 cgaggaagat ctggcgtgcc ttgcgggcca acggacaagc ccattcggat gaagcggatg
     1141 tcgcttcgcg catcgccatg ctgctcgacg aagcttcggc tgccgccgtc gatgcgagat
     1201 cgcgggcgca gctgacggat gcggccatgc gcctccgcgc cttctcggga gcaaatgcgc
     1261 ggcgtgcgcg gctttattca gaccggaatc ctctccggcg catcggcgtg ctgaagcagc
     1321 ttctgctgga tggcgcctat cggagcagcc ccgcgccgag tttcggtccg cgagcgttcg
     1381 tcaaggacct cgcctacgcg ctgttccgtc ccaactgaac ctgtaagccg gccgcggacg
     1441 tgcggcgggc ttgcagcgcc tgcatcagcg ccagcaacag ggtcccgcga tgcggatccc
     1501 atttcagcaa gcgcagaaga taggcggcac ggcgcgcgtg tcgccaggcg ggagaccgga
     1561 gctcgcgccg catcgccgcc agctcgaacg gctcgagcca gctcgatctc gctgccagtc
     1621 gctcgatcaa agccggcggg atcggaaggg cgaaagcgcg taggtaagcg aggccgatct
     1681 gcagccgctt cgtgaggccc tgttcctccg cgaacgcctc gatcgccgcc caatcgatag
     1741 cctcgttcga acccaggatc atcgcggcgt cggccaccca ccggaacggc ggtgaaggat
     1801 tggacctcaa gccatgaacg atggtctgga gaaggaggtc gctcgcggcc ggacggagcg
     1861 agcgggcact tcccacgtcg atcggcaccg cacgcgacca gaaatagctg tcggcggctc
     1921 cgttggggca atcgtacaag acgtgccagt ggaggtcgac ctcggtgccg tcggcgcgca
     1981 cgtgcatcag cgcatgatga agacggcgct cggtcgccca gctttgacgc ctgacggaaa
     2041 agccatctct ctcgagcaat gcgctcgctc ttggcgcgtc ctcgaacggc acgatgatgt
     2101 cgacgtcgct catcgggcgc agcgccggtg cgggataata agcgagcgcg agcggcaggc
     2161 ccttgccgat catcgtcccg atgccctcgg cttccagcgc ggcaaggatc gcagcggcgc
     2221 cctggatgcg ccgcgtcatc ttcacccaat tgctgcggaa tacccccttg aggcgcggca
     2281 ggagaggatg cgggacggag gcctgcgaaa gccgtttcca gacgagcggc agcagccgaa
     2341 acgacccttc gtcatgaggc ccgtcgaacg gctcgaccgc aagccattgc tcgaacagcg
     2401 cgcatgcctc ctcgccgtca cgagcaagcg cggcccggag aagaagctcc tgcgcgagcg
     2461 agggaagcag cgcatcacgc cgggcgggca tcggctcggc ggtccgcgcg tttgtgacgg
     2521 agagccatgc ggagtgcccg cagatactcg gtttcgagcg tctgcgtatc gtgcaccgag
     2581 ttcgtcgcat gcacgcgccg tcgcagcacg acatcctgta tcgaagccgc gttcattccg
     2641 gcggcctccg cgcggctgac ccactccatc atatagccca tgcgcaatgt cgacgcgaag
     2701 gcgccgacgc gctcgaatgc cgaacgccgg aacaagcttg cgccggtcat ccggccggtg
     2761 agcgtctgca gccgcgccgg aagccgggat ttctgctccg gaccaagatc ggggctgatg
     2821 aactgctcga ccgtcccgta cacataagcg agctcgtcgc gtttcgagag caaggcgagt
     2881 cgcagcgcga tgctgtccgg ggggagcagg tcgtcatgat ccagaaagca gatgaacgtg
     2941 cctttggcgc ggcttatccc gacattctgc gctgccgcca ggccccgtgg ttcctggcgc
     3001 agacagacta tgtcgccgaa cccgcgcgcg atcgttccgg tcgcgtcggt cgagccgtcg
     3061 tcgaccacga tgatctcgtc gggccgccgg ctctgcatga ccgccgacgc gagagcttcg
     3121 ccaagatagt ttgcgccatt ccggaccgcc aggatgagag ataatgttcc cattccggtc
     3181 ggccgcaccg gccgcctcag acccgggcct gtcgcggagc gaccggccag cccgcctcgt
     3241 cgacgtcgtg gatcggatcg agcaggagca gatcctgcat gtcggtgaag atttccagcc
     3301 gaggctccgc atataggcag gccggcagcg ggatagccga aagatccgca tcctgccccg
     3361 gaggagccgc cgcccgcacc agctcgtggc tcagcagctc ttcgaacagc gccccgaccg
     3421 tctgccgcgc atgggccgcc tcaagtccga aatgcgatgc gagcgcatcg gcgatcgcgc
     3481 ttgccttcat tcgattttcg acaccgcccc agatgagcgc accgcttcct tccaggctat
     3541 aataatagcc ccgcttgaga tgcatgatga tggcctcgcc gtcgacgatt tctcccgcca
     3601 cgtccggcgc gttgacctcg aaggcttgct caatgctgtt ctcggcgccc atcggcccaa
     3661 tgggtagcga cggactggaa tccgcgcaag caaggcgccc cacggcgatt gattccgcgc
     3721 agacgcgggg atagcgcatc ccagaattga acgagggcgc ggcgatcgtc gaaaccagtg
     3781 tcatcatcgc cgtccgcaac ggcgaacgct tcctcggtga agcgatccgg tcggtcctcg
     3841 cgcaggtctc cgcgaccgac gagctgatcg tggtggacga cggctcgacc gaccgatcgg
     3901 cgcaaatcgc cgcctcgacc gccgatccgc gtgtgcaact gatccaacag gaccgacgcg
     3961 gcgtttccgc cgcccgcaat cgcggcgtct cggcggcgaa cggctgcttc ctcgcattcc
     4021 tcgatcatga cgatctctgg ccttcggaac ggcacgggat cttgatcgaa gcgctttacc
     4081 aaaacagg
//
LOCUS       DASZMC010000276         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4249575,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000276 DASZMC010000000
VERSION     DASZMC010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4249575"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VG274_05640"
     CDS             <1..359
                     /locus_tag="VG274_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06169.1"
                     /translation="LTKALTEENAKSPAAAPADVRSTVCDLAWPDTEGDKRQDLELSA
                     KTPGSAGIEVAGESLEDFPSAQADNPSDAYGEEIEPIPETYFRQEAEWCESHASRSDE
                     PEWAHETVEISELEWT"
     gene            complement(1096..1254)
                     /locus_tag="VG274_05645"
     CDS             complement(1096..1254)
                     /locus_tag="VG274_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWE06170.1"
                     /translation="MSLRELAAQAGVSGMAPYRHFEDKAALMQAAAQRGFALFAQEPR
                     GWPGQARP"
     gene            1283..2494
                     /locus_tag="VG274_05650"
     CDS             1283..2494
                     /locus_tag="VG274_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HWE06171.1"
                     /translation="MRLPLKNDFLAAQWLRTAGHAAAGGADLAECFLAAERVRETDPE
                     SWRSAWFGVADRVAGEAAKSAAIGCDVSAHGSWLRAANYYRAAGLFLLQRNEASRLRQ
                     VLRRQREAFASAMAARPGWAEAFEIPYQGRTLHGLFFRADGPGPRPTLIMTGGYDSTA
                     EEAYFFSGAAALARGYHFVSYDGPGQGRALEEGLVFRPDWEVVMSAVVAAVEARPEVQ
                     SNRIVSMGLSFGGYLAPRAATGCPTLAALLADPGQLSLLDEARTRLPGPLARALPDGN
                     RLWLGALERILEWRRKDLIKGWALRRGMFVHGVETPIAYLKILAEYTSEGRLGRIRCP
                     VFVASAEADEIGATAKRLFNEIETRKSFQPFRVEEAAGEHCESGARSLFNQRAFDWLA
                     ETFAAARERGA"
     gene            2667..>3097
                     /locus_tag="VG274_05655"
     CDS             2667..>3097
                     /locus_tag="VG274_05655"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWE06172.1"
                     /translation="MAEHQRKSSPPPRLPGARFLVVEARYYDAVGQMQLAGVRAAFEA
                     AGASFDLISAPGALEIPAAAAIALDAAADKGVTYEGLVALGCVIRGETYHFEIVAGES
                     ARALMDMSVARKLPLGNGILTVETLPQAEERADPARGDKGG"
BASE COUNT          539 a          986 c         1004 g          568 t
ORIGIN      
        1 cgctgacaaa agcccttacc gaggagaacg ccaaaagtcc ggctgcggcg ccagcggacg
       61 ttcgatcgac ggtctgcgat cttgcttggc cggacaccga aggagataag cgacaggatc
      121 tggagctctc tgcgaaaact ccgggctcgg caggcattga ggtcgccggc gagagtctcg
      181 aagattttcc gtcggcccaa gccgacaatc cgtcggatgc ttatggcgag gagatcgaac
      241 ccatccctga aacctatttc aggcaggaag cggagtggtg tgaatcgcac gcttccagat
      301 ctgacgagcc ggaatgggca cacgaaacgg tggagatttc agagttggaa tggacctaag
      361 ccgttgcaca ccaatcccct caggttgtgt caggcgcgag gcgctctgcg ggctcgtatc
      421 cgcagatgtt gaagcctttc gcgcattgcc tgccgaggcg agccagtaca tggctcttag
      481 agcctgtttg gctccggaaa tcattgggaa tttcgcactt ttgcgatgcg tgtcacaagc
      541 aaaatttgcg ggcggtgatc gtcagcatcg ggtttccctt tgaattcagt actacagtgg
      601 aattcaggcc gatccgccgt caaaccttct tctcgaaacc ccagcgtttt gcggagtcaa
      661 acaggctctg agcgacctgg cccatttcga cgatttcgac cgtgtctact gcggattctt
      721 ttcagatccc ctgccctgtc gcacgacagt tcaggccgga ctggatggca ttctcgtgga
      781 aatagacgcc ctcgcctatc tcacgccgga gccgggcgct gccaaatcgg attgagcttg
      841 gtgcggggcg gtctccataa ccgcagctta aggttcgttg ttcaggaggc gagtcaaaac
      901 cctgtccgtc accgccgaag cagttggacg ccgctcaagc gcaaggccga gggttcgcca
      961 gacataccta tccagagcga accgcctggc ggggaggcga gatcatggct tcgtcgttgc
     1021 gaaaatctga tgcgcccgaa ctgagcgcct gataccaagc cgcgcaatat ccaacctgtc
     1081 aagaacgcct gtccgtcatg gccgggcttg tcccggccat ccacgcggct cctgtgcgaa
     1141 cagcgcgaag cctcgctgcg ctgccgcttg catcagcgcc gccttgtcct cgaagtggcg
     1201 atacggcgcc atgcccgaca ctccggcttg cgccgccagc tctcgcagcg acaagctgcc
     1261 tattcatggc aaggaaaacc tcatgcggct gcccctcaag aacgacttcc tcgccgcgca
     1321 atggctgcgc acggcggggc acgcagcggc cggcggcgcg gatctcgccg agtgcttcct
     1381 cgcggcggag cgggtgcgcg agaccgatcc ggaaagctgg cgaagcgcat ggttcggcgt
     1441 cgccgatcgt gtcgccggcg aggcggcgaa aagcgcggct atcggatgcg acgtcagcgc
     1501 gcacggctcc tggttgcgcg ccgcgaatta ttaccgcgct gcaggcctgt tcctgctgca
     1561 gcggaatgag gcttcgagat tgcgacaggt tctgcgccgg cagcgcgagg cgttcgcctc
     1621 ggccatggcg gcaaggccgg gttgggccga ggcgtttgaa attccttacc agggccgcac
     1681 gctgcatgga cttttctttc gcgccgacgg cccggggccg cgcccgacgc tgatcatgac
     1741 gggcggctac gacagcaccg cggaagaagc ttatttcttc agcggcgccg ccgcgctggc
     1801 gcgaggctat catttcgtca gttacgatgg gccgggccaa ggccgcgcgc ttgaggaagg
     1861 gctggttttt cgacccgact gggaagtcgt gatgagtgcg gttgtcgcgg cggtcgaggc
     1921 gcggccggag gtccaaagca accgcatcgt ttccatgggt ttgagtttcg gcggctacct
     1981 cgcgccgcgg gccgcgacgg gctgcccgac gctcgccgcg ctcttggcgg accccgggca
     2041 attgagcctg ctcgatgaag ctcggacgcg cttgccgggt ccgctcgccc gggcgctgcc
     2101 ggacgggaat cggctgtggc tcggcgcgct cgaacgtatt ctcgagtggc ggcgcaagga
     2161 tttgatcaag gggtgggcgc tgcggcgcgg gatgtttgtc cacggagtcg agaccccgat
     2221 cgcctatctg aagatcctgg ccgaatacac gtccgaaggc cgtctcggtc gcatccgctg
     2281 ccccgtgttc gtcgcctcgg cggaagccga cgaaatcggc gccaccgcca aacgtttgtt
     2341 caatgaaatc gagacgcgca agagcttcca accctttcgc gtcgaagagg cggcgggaga
     2401 gcattgcgag tcgggcgctc gatctttatt taaccagcgg gcgttcgatt ggctggccga
     2461 gacttttgca gccgcacgcg agcggggggc gtgatcgccc gtgagatcgg cctcgggagc
     2521 atgcctggcg gtgatcgcgt gtcttgtcga aatggctttt gtgacaagtg agaaatccaa
     2581 acggaaacat tgcccggcgg cgcctcggct tgcgtcgccg cgcccggccc tctaggagtg
     2641 ccccccttcc tcgaccctag cgagccatgg ccgaacacca gcgcaaatcc tcccctccgc
     2701 cgcgcctgcc cggcgcgcgc ttcctcgtcg tcgaggcgcg ctattacgac gccgtcggcc
     2761 agatgcagct tgccggcgtg cgcgccgcgt tcgaagccgc gggcgcatcg ttcgatctca
     2821 tctcggcgcc cggcgcgctc gagatccccg ccgccgcagc catcgcgctc gacgccgccg
     2881 cggacaaagg cgtcacttac gagggcctcg tcgcgctcgg ctgcgtgatc cgcggcgaga
     2941 cctaccattt cgaaatcgtc gccggcgaat ccgcgcgcgc gctgatggac atgagcgtcg
     3001 cccgcaagct gccgctcggc aatgggattc tcaccgtcga gaccctgccg caggccgagg
     3061 agcgcgccga tcccgcgcgc ggcgacaagg gcggcga
//
LOCUS       DASZMC010000277         7806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_682762,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000277 DASZMC010000000
VERSION     DASZMC010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682762"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VG274_05660"
     CDS             complement(<1..387)
                     /locus_tag="VG274_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06173.1"
                     /translation="MLLVRRFDDTDYSGDFRGVVRTPTRAGDLAGSIGFGHASIAHRP
                     RISDVATGNLFLAIAILTLLRFILAASLPLSFDESYYWVWSKHLALGYYEHPALIALA
                     IRAGTMIFGDTEFGVRFIPLSTSLVAS"
     gene            577..2088
                     /locus_tag="VG274_05665"
     CDS             577..2088
                     /locus_tag="VG274_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWE06174.1"
                     /translation="MPRSVLLPISRLFRTASFKLAAAYVLLFGASVAVLAAIVYLTAT
                     AALDRQERTRLHAEATALRAEYRRGGFEKLMEEIHSRQRAELVGGLDYTVVAASGARL
                     FGNIPALKLRSGWAKVKGPPDGDEPPGHRERLLVYSVQLSPGLWLQVGDDIGRQVLVG
                     KAIMATSGWVLLVIVTLAIAGGIVLSASVLSRIDGITRTAEAIIEGDIGRRVPMRGVD
                     DDIDRLSATLNRMLDRISSLMGALRQVSRHIAHDLRTPLGRLRQGLDEARGNATTLGE
                     YERAVESAVGETDQILETCAALLRIAEIESGTRRAGFRAVDLSALALQIGQTYVPAAE
                     DAGKQLSLAIAPGVSVDGDRELLAQLLVNLIENALRHTQDAAGILVELAAGRGAPVLA
                     VSDNGPGIPGEERDRVIGRFYRLERSRATEGSGLGLSLVAAVADLHRAELVFTDRAPG
                     LSVSIAFSERAGTARPLTEMRAGALHRVPMLASLASLLGSGRPTAPAGGKPGI"
     gene            2085..2678
                     /locus_tag="VG274_05670"
     CDS             2085..2678
                     /locus_tag="VG274_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HWE06175.1"
                     /translation="MSLPLANTSARYGGVAMTLHWLIAGALIVNIGLGLYMSEVLSNG
                     DASRLAILPLHKSVGLTVLLLSVVRLSWRLVNPVPPLPESMPPVLRIAARASHVFLYF
                     LIVAIPLTGWALVSASRTAAPTLYFGVFHWPNMSFLAQLPRAEKIPLHRGFGLVHITL
                     AWSAILLVPLHVFGALYHHWRGDDVLRRMLPGTGIAG"
     gene            2668..3495
                     /locus_tag="VG274_05675"
     CDS             2668..3495
                     /locus_tag="VG274_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06176.1"
                     /translation="MRDRSRDAHCNGSGWGLRLDPKPFRLAAAALVPFVLSGPPAIAQ
                     TDEIQVYNAEIAAPGVFNLTLHDNYTPDGRTVPAFPGGLAPNHTLNGVPEWAYGVTDW
                     FEAGLYLPLYSISSNEGAVLNGFKLRALFVSPDAADRSFFYGINFEFSYNARHWDPNR
                     YTSEIRPILGWRFGPVDLIFNPILDNSYKGVSRLDFAPATRLAYNFSKAWAVAAEEYD
                     DFGPLRGFLPASQQSHQLFAVADWSGEALSVEGGIGFGLTSATDHLVLKLILSKDLD"
     gene            complement(3885..4700)
                     /locus_tag="VG274_05680"
     CDS             complement(3885..4700)
                     /locus_tag="VG274_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWE06177.1"
                     /translation="MTGGKTTVEFGWRVFGLGVMALALVSLAWGNFDTGQPVPKDFPD
                     RTALAYAAAAFMFVGGAAVEWRRTAAWGAAALTAYYTLIVVVLMNGRIVLRHYAEFGA
                     YDGAAEQLAIAAAALIVYAANANIDAALAARLTRLGQVTFGVCAVLFGGAHFFYMNLT
                     IPLVPKWLPPSQVFWAYATGIGHIAAGLAILTGVQARLAAILLTVMYASFTPLVHVPI
                     LLADHSKYFTWTENALNLSLTGAAWVVADSLARPRRRRSDAIARLVTHGSTTC"
     gene            4897..5931
                     /locus_tag="VG274_05685"
     CDS             4897..5931
                     /locus_tag="VG274_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06178.1"
                     /translation="MKSNPSLSARMLKSSLLAIGAAALACALAGTAAARIVTIDPPNS
                     IATYAWSLNDKGEITGFYFDGTSGSTKGFVRKADGVITTFEHPTSAVTAAYGIDSRGR
                     ATGYWSTDDGNFPGFVRTRKGTLKPFSVSEAVTGTFPVSMNANSSIAGAWYDAYSVPH
                     GFLRTSDGTITTFDVSGDAYGTFPTGINAGNAIAGHYGDSNGFNHGFLRAQDGTIAAF
                     DGAKNASQTFALGINTTGTIAGYFQDSSGVTHGFARGSDGTISSFDLKGSTGTFAESI
                     NDNGDITGYYEDSGGLAHGFVRSGDGSFASFDPQGSTGTFANYINGNGHIVGFYTDAT
                     GDYHGFLRIP"
     gene            complement(6272..6520)
                     /locus_tag="VG274_05690"
     CDS             complement(6272..6520)
                     /locus_tag="VG274_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06179.1"
                     /translation="MSDEDLVVIPLPTNKGTVTWAVARLGSEGDRAFAEAIVAIPETD
                     FAPPRRIALSPYSLARIRPGSPHRPNLYLYEGMIMPPL"
     gene            6930..>7806
                     /locus_tag="VG274_05695"
     CDS             6930..>7806
                     /locus_tag="VG274_05695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWE06180.1"
                     /translation="MSAGATGALWRARAPFCAAIAGCIALFACAPAVVQTKPAAVAMV
                     PAPPTIWPTRAWPTSTPEAQGIDSGKLADALEQIRARKIPVHSLLIERHGHIVLDSYF
                     YPFADNEAHNAYSVTKSVTASLIGIALREHRLGDLNAPVLSFFPDRKNDDPRKAQITL
                     AQLLSMTSGLDCRSQGGRNLLQQMLASPDWTTFMLKRDVVAAPGSTFGYCGGNAELVS
                     AVLTERTGTSARDFARRELFAPLGITDAGWPTSACRVSHGWGDLELQPRDMAKLGYLW
                     LHDGSWEGREIIPADY"
BASE COUNT         1449 a         2427 c         2439 g         1491 t
ORIGIN      
        1 gcttgctacg agcgaagtcg acagcggaat gaaacgcacg ccgaattcgg tgtcgccgaa
       61 aatcatcgtt cccgcgcgaa ttgccagtgc aatcagcgcc ggatgctcgt agtagccgag
      121 agcgaggtgc ttcgaccaca cccagtagta ggattcgtcg aacgagagcg gcagcgatgc
      181 ggcaaggatg aagcgcagga gggtgaggat cgcgattgcg aggaacagat tgcccgttgc
      241 gacgtccgaa atgcgcggcc gatgtgcaat cgatgcatga ccgaatccga tcgagccggc
      301 gaggtcgccc gcgcgcgtgg gagttcggac aacgccgcgg aaatcacccg aataatcggt
      361 gtcgtcgaat cgccggacca atagcataca ctctgcgcat gcagccggcg cctccggctg
      421 cccgaacaaa cgaaatcccc gatctgatca tgcctggtcc gacattgcag gacgcgttca
      481 cgaatgttgg gaatttgtat gggaatgcgg acgggggatc tgcgccgcgc aattctttcg
      541 ccaaagggta atccccgtta tgctcgataa tgcgacgtgc cgcggagcgt tcttttgccg
      601 atctcccgtc tgttcagaac agcaagcttc aagctcgctg ccgcttatgt gctgctgttc
      661 ggcgcgtcgg tcgcggttct cgccgccatc gtctatctta ctgcgaccgc tgcgctggac
      721 cgccaggagc ggacgcgcct ccatgccgag gcaacggcgc tgcgcgccga ataccgccgt
      781 ggcggtttcg aaaagctgat ggaggaaatt cattcccgtc agcgcgcgga gctggtcgga
      841 ggcctcgact acacggtggt tgcagcatcg ggagcacgat tattcggcaa tattccggcg
      901 ctgaagcttc gaagcggatg ggcgaaagtg aaaggtcctc ccgacggaga cgaacccccc
      961 ggtcatcgcg agcggcttct ggtctactcc gtgcaactat cgcccggtct gtggcttcag
     1021 gttggcgacg acatcggcag gcaggttctg gtcggcaaag ccatcatggc gacatccggc
     1081 tgggttcttc tcgtcatcgt cacgctcgcc attgcgggcg gcatcgtgtt gagcgcgagc
     1141 gtgctctccc gcatagacgg aatcacgcga acggcggaag ccatcataga gggtgacatc
     1201 gggcgccggg tgccgatgcg gggcgtcgat gacgacatcg accggctttc ggccacgctc
     1261 aatcgcatgc tcgaccggat ttcatccttg atgggcgcgc tgcgccaggt gtcgagacat
     1321 atcgcccatg atttgcgcac gccgcttgga cgactgaggc aggggctcga cgaagcgcgc
     1381 ggcaatgcga caacacttgg cgaatacgaa cgcgcagtcg agagcgccgt cggagagacg
     1441 gatcagattc tggaaacctg tgcggccctg ttgcgcatcg cggaaatcga gtcgggaacg
     1501 cggcgcgccg ggtttcgcgc cgtggatctc agcgcgctcg cgcttcagat cggccagacc
     1561 tatgttccgg cagccgagga tgcaggcaag cagctgagtc tcgcgatcgc tcccggcgtg
     1621 tctgtggacg gcgaccgcga gttgctcgcg caattgcttg tcaatctcat cgagaacgcg
     1681 cttcgtcaca cgcaggatgc cgccgggatt ctcgtcgagc tcgcggccgg gcgcggcgca
     1741 cccgtgctgg ccgtgtcgga taacggaccg gggattcccg gcgaagagcg cgatcgcgtc
     1801 atcggaaggt tctatcgcct cgaacggagc agggcgaccg aaggaagcgg acttggcctg
     1861 agcctcgtcg ccgcggtggc cgatctccat cgcgcggaac tcgtttttac cgatcgggcg
     1921 cccggccttt cagtgagcat cgccttttcg gaacgggccg gaaccgcgcg gcccttgacg
     1981 gaaatgcggg ccggcgcatt gcatcgcgtc cccatgcttg caagccttgc atcgctgctc
     2041 ggatcgggcc gccccaccgc gccggccggc gggaagccgg gaatttgagt ttgccgctcg
     2101 ccaacacctc cgcacgctac gggggcgttg cgatgaccct tcattggctg atcgcgggcg
     2161 cgctgatcgt caatatcggg ctcggtctct acatgtccga ggtgctgtcc aatggcgacg
     2221 cttcgcgcct cgcgattctt ccgctgcaca aatccgtggg cctgaccgtg ctcctgctga
     2281 gcgtggtgcg attgtcatgg cgtctggtta atcccgttcc accccttccc gaatcgatgc
     2341 cgccagtcct caggattgcg gcgcgcgcat cccacgtctt tctctatttc ctgatcgttg
     2401 ccatccccct gacgggatgg gcgctcgtct ccgcgtcgcg gaccgctgcg ccaaccttat
     2461 actttggcgt tttccactgg ccgaacatgt cgtttctggc ccagttgccg cgggccgaga
     2521 aaattccgct gcaccgcggc ttcggtcttg tgcacattac gcttgcctgg tcggcgatcc
     2581 tgcttgtgcc gttgcatgtc ttcggcgccc tgtatcatca ttggcgcggc gacgacgtgc
     2641 tgcggcgcat gctgccgggg accgggattg cgggatagat ccagagacgc gcattgcaac
     2701 gggagcggat ggggccttcg cctcgatccg aagccgttca ggctcgcagc ggcggcgctg
     2761 gtcccttttg tattgtcggg gccgccggcg atcgcgcaga ccgatgagat ccaggtctac
     2821 aacgcggaga tcgcggcgcc gggcgtgttc aatctcacgc ttcacgacaa ttacacgccg
     2881 gacgggcgca cggtgccggc gttcccgggc ggactggcgc ccaaccacac gctgaacggc
     2941 gtgcccgaat gggcgtatgg cgtgaccgac tggttcgagg ccggtctcta cctgccgctc
     3001 tattccatct cgagcaacga aggcgctgtg ttgaacggat tcaaattgcg cgcgctgttc
     3061 gtgtcgccgg atgcagccga ccgcagcttc ttctacggca tcaatttcga attcagctac
     3121 aatgcgaggc actgggatcc caaccgctac acctccgaaa tccgccccat cctgggctgg
     3181 cgcttcggac cggtcgatct cattttcaat ccgatcctcg acaattcgta caagggcgtg
     3241 tcgcgtctcg atttcgcgcc ggcaacgcgg ctggcttaca atttttcgaa agcgtgggcg
     3301 gtggccgcgg aagagtatga cgatttcggc ccattgcgcg gatttcttcc tgccagccag
     3361 cagagccatc agctcttcgc cgtcgccgac tggagcggag aagctctcag cgttgaaggc
     3421 ggcatcggct tcggcctcac ctccgccacc gatcatctgg tgctgaaact catcctttca
     3481 aaagatctcg attaaggaag caaggaatca gcgcagagga cgcagagggt gcgcggtgga
     3541 cgcggggaag aaagaatcac gcgccttcct cctacgctgc tccgcagctt cggaggactg
     3601 ccactctcgc ccgccggagc cttggcgaag gcgggtcggc gcgcaacgtc aacctctgcg
     3661 tgccttgcgc taaaatcttt ttgcgttcgt caatacatga cattcggtgt catgtttggt
     3721 acgctgtgtc tcttgcccgc aatccggaga atcaacggag acagtggagg acggtgagta
     3781 tctgttgcgc gcgcgtggcg cgccgttctc ctctcactgt cctcactgtc tccctgtgaa
     3841 tccttccttc ggcgcggcct ccgcgtcccg gtgtgggcgg tggatcagca ggttgttgag
     3901 ccgtgcgtca ccagtcgcgc tatggcgtcg ctccgccggc gcctcggccg cgccagcgag
     3961 tccgccacca cccaggcggc gccggtgagg gagagattca gggcgttctc ggtccacgta
     4021 aaatatttgg agtgatcggc caggaggatc ggcacgtgca ccagcggcgt gaacgacgcg
     4081 tacatgacgg tcagcaggat cgcggcgagg cgggcctgca cgcccgtcag gatcgcgagg
     4141 ccggccgcga tatggccaat cccggtcgca taagcccaga acacctgact cggcggcaac
     4201 catttgggga ccagcggaat agtgaggttc atatagaaaa aatgcgcgcc gccgaacaac
     4261 accgcgcaaa ccccgaacgt cacttggccc aggcgtgtga ggcgcgcggc cagcgccgca
     4321 tcgatgttgg cattggcggc gtagacgatc agcgccgccg ccgcgatcgc gagctgctcg
     4381 gcagcgccgt cataggcgcc gaactctgcg taatggcgca gcacgatacg gccgttcatc
     4441 aggacaacga caatgagcgt gtagtaggcg gtgagcgccg cggcgcccca ggccgcggtg
     4501 cggcgccact cgacggccgc gccgccgacg aacatgaacg cagccgcggc ataggcgagc
     4561 gcggtgcggt cgggaaaatc cttgggcacc ggctgccccg tgtcgaagtt gccccatgcg
     4621 aggctgacca atgccagcgc catcacgccc aggccgaaga cgcgccagcc gaattccacc
     4681 gtcgtttttc ccccggtcac ggtttttccc cttccaatgc aaacgcgcgc agagcgatga
     4741 tcgggccgta caataccatc aagctcggcg accgattgcg tattgcgcgt cgcccgcttc
     4801 tcacatctgc gttacggcat aaaccggctg cgaatcgccc ggcttcgtgc tagaattgct
     4861 ccgcctgatg ccgataacca gaaaagcgga aacaacatga aatccaaccc gtcgctttca
     4921 gcccgtatgt tgaaatcttc gctcctggcg atcggtgcag ccgctttggc ctgcgctctg
     4981 gcgggcacgg ccgcggcaag aatcgtcacc atcgatccgc cgaattcgat cgccacctat
     5041 gcatggtcgc tcaacgacaa gggtgaaatc accggctttt atttcgatgg aaccagcggt
     5101 tctacaaagg gttttgtgcg caaggcggac ggcgtcatca ccacttttga gcatcccacg
     5161 tcggcagtga cggccgctta cggcatcgac agcagggggc gcgccaccgg atattggagc
     5221 accgatgacg ggaattttcc ggggttcgtc cgcacacgga aaggaacctt gaagccattc
     5281 agcgtgagcg aggcagtcac cggtacgttt ccggtgagca tgaacgccaa cagctccatc
     5341 gccggggcct ggtacgatgc ctattccgtg ccccacggat tcctgagaac ctccgatgga
     5401 acaatcacca ccttcgacgt atcgggtgac gcctacggca ccttcccgac cgggatcaac
     5461 gccggaaacg ccatcgccgg tcactatggc gacagcaacg gattcaatca tgggtttctg
     5521 cgcgcacagg acggcacgat agcggccttc gatggcgcca aaaacgccag ccaaaccttt
     5581 gctctgggga tcaataccac gggaacgatc gccggttatt ttcaggattc gagcggcgtc
     5641 acgcacggat ttgcgcgggg ctccgacggc acgatttcga gtttcgatct gaagggttcg
     5701 accggaacct ttgctgaaag catcaacgac aatggcgaca tcaccggtta ttacgaggac
     5761 agcggcggcc tcgcgcacgg ctttgtacgg tcgggcgatg gaagctttgc ctcattcgac
     5821 ccgcaaggct cgacaggaac cttcgccaac tacatcaacg gcaacggcca tatcgtcggc
     5881 ttttataccg atgcaactgg cgactatcac ggcttcctgc gcatcccctg aggcgggtcg
     5941 cagcaatacg gggacatgtc atgcattgcc tgtcgccatt ttccgccggc cgagcgaccc
     6001 ctcacccatt tcgaagcggt agcgaggact ggaatgtttc ccctctccca caaggggaga
     6061 ggggagtctg cgtgctgatt ttccggcatt gatgcaagac tcgggattcc cctctcccct
     6121 tgtgggagag ggatgcagat acgtgcaatg atttctgctc aggcacgggt gagggggctt
     6181 cgtgagcgaa ttccgcaaca gataatgcgc tacctgtcgt ctgtacccac cggatagcag
     6241 ctatttttta gggtcaaagg ccgcatcggc gttacagcgg cggcatgatc attccctcgt
     6301 agaggtacaa attcggccga tggggagagc cgggccggat gcgagccaag ctgtaaggag
     6361 atagcgcgat gcggcgcggg ggtgcgaagt ctgtctcggg gatcgccacg atcgcctcgg
     6421 cgaaggcgcg atcgccttcg ctgcccaacc gggccaccgc ccaggtcacg gtgcctttgt
     6481 ttgttgggag cgggatgaca accagatctt cgtcggacaa ttcattctcc tgatcgggtg
     6541 aagatgaggg gacgcttcgt gaggaccatg ggagcatcgc gcgcgccgcc ccaactatca
     6601 aatgatgatc gtcgtgctaa atcgttacaa atgcggcgaa atcggatttt tccattgacc
     6661 ttttgccaat cgctccgcta cgaaaaccgc gctcgatgat gatcgcgatt cgccaacgca
     6721 agagtggctc gccgatcgct cgcagccttc gggctgggta cgcaaacacg aaactgaatt
     6781 cgcggcgcgg gccgccggat tacatcggga ggtcgaccct ccctggcgcg cgcgcgcagg
     6841 ttttcgaagc gctttcccgc tgatcgggaa agtgctggcg caggcgcccg atctggcgaa
     6901 agcgtcgcga aagagtcgga ggggagttca tgtcggcagg cgcaaccggc gcgttgtggc
     6961 gcgcgcgtgc accattctgc gccgcgatcg ccggctgcat cgccctcttc gcctgcgcgc
     7021 ccgcggtggt ccagacaaaa cctgcggccg ttgcgatggt gcccgccccg ccgacgatct
     7081 ggccgacgcg agcctggccc acctcgacgc cggaagccca gggcatcgac tccggcaagc
     7141 tggccgacgc gctcgaacaa attcgcgcga ggaagattcc ggttcacagc ctgctgatcg
     7201 agcgtcacgg tcatattgtc ctcgacagct atttctatcc ctttgccgac aacgaggcgc
     7261 acaacgccta ttcggtgacc aagagcgtga ctgccagcct gatcggcatc gctcttcgcg
     7321 agcaccggct cggcgatctg aatgcgccgg tcctgagctt tttccccgac cgcaagaatg
     7381 acgacccacg caaggcgcag atcacgcttg cgcagctgct cagcatgacg tcggggctgg
     7441 actgccgttc gcagggcgga cgcaatctgc tgcagcagat gctggcgagc cccgactgga
     7501 ccacattcat gctcaagcgc gatgtcgtgg ctgcgcccgg cagcaccttc ggctattgcg
     7561 gaggaaatgc ggaactggtc tcggccgtgc tgaccgaacg gacaggcacg agcgcgcggg
     7621 atttcgcgcg gcgcgagctt tttgcgcccc tcgggatcac ggatgcggga tggccgacaa
     7681 gcgcgtgccg cgtttcccac ggctggggcg atctcgagtt gcagccgcgc gacatggcca
     7741 agctcggcta tctgtggctg catgacggca gctgggaggg ccgcgagatc attcccgcag
     7801 attatc
//
LOCUS       DASZMC010000278         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2867103,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000278 DASZMC010000000
VERSION     DASZMC010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2867103"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..568)
                     /locus_tag="VG274_05700"
     CDS             complement(119..568)
                     /locus_tag="VG274_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06181.1"
                     /translation="MSSPDLAETRRALRREWLIARIVVIGGFVIAIAAGGWFGVIVPE
                     IIKPRQEHTALVNRITNLLNAEAQICTMALGSAKNFGIVPQYGQLASAKLALTRVQGR
                     YLCIATTSAAKYVLAVDLVCRELKNPRCTSLYNVSQADGTVLYQRQR"
     gene            complement(590..2089)
                     /locus_tag="VG274_05705"
     CDS             complement(590..2089)
                     /locus_tag="VG274_05705"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWE06182.1"
                     /translation="MRELAHFIGGRFAKGRSGRFGDIYNPATGEVQARVPLASRSEVR
                     AAVETAAKAFREWSGVNPQRRARVMFDFKALVERNIDELAHLLSSEHGKVIADSRGDI
                     QRGLEVIEFACGIPHLMKGEYTEGAGPGIDIYSMRQPLGVVAGIAPFNFPAMIPMWMF
                     GVAIACGNTFICKPSEKDPSVPLRLAELFMEAGAPAGVLNVVHGDKEAVDAILEDPDL
                     QAVSFVGSSAVAEYVYSHGTARGKRVQAMGGAKNHAIIMPDADLDQVTNELIGAGYGS
                     AGERCMAISVAVPVTDKLADALVERLKPRVECLKVAPPTDPDADYGPMVTAEHRQKVL
                     SYIELGVKEGADLVVDGRGFSLQGYENGFFLGGSLFDRVTPGMKTYRDEIFGPVLQVA
                     RAPDFDAALALPSNHQYGNGVAIFTRDGDCAREFASRVKVGMIGINIPIPVPLAYHSF
                     GGWKRSAFGDVNQHGPEGVRFFTRIKTVTQRWPRGGLRERPDFSIPTMK"
     gene            complement(2226..2576)
                     /locus_tag="VG274_05710"
     CDS             complement(2226..2576)
                     /locus_tag="VG274_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HWE06183.1"
                     /translation="MTRLSALAIGAVLAFSTVALAHADQTIAGVWKLSVGENDAPCIL
                     TLAPDQTADSAGIATPSPDCAGGLNAIGRWKATPTGLQLFSPSGDMVAWLKSKDGAYE
                     GSRLSDGRKLALNR"
     gene            3090..>3935
                     /locus_tag="VG274_05715"
     CDS             3090..>3935
                     /locus_tag="VG274_05715"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWE06184.1"
                     /translation="MTSVSHVAGDAAPGGIWRWAALALFLIALAAAGLAVATAGASGW
                     SGVALLAGIGSIAGLFLAVIWPRANVAAADARRVAEAAAKANVAWAITNRDGVVIHSN
                     EAYRRLAGAREREAPPPPELALQGESAASVLYRLSRKARAGQSHEEILQAASGLELLV
                     AVRPLSDGRAAWWFTPRVAAREAASIATAPAGAGSASGLSGGAAMFRDAPIGIAIAEP
                     DGTLVETNALFQQFFGAAGDLGGRGLADLAAPPDRADIAETFVRAAAGESAAFGLRAA
                     SGVESL"
BASE COUNT          684 a         1266 c         1280 g          705 t
ORIGIN      
        1 gaatggcgat gcgggataat ggcgtccgct tttgtcactc cgacgcggaa attctcgcat
       61 cggaggggcc cgcacatcgc gttgggtcca ccgggacgac cggtccggcg cagtgcgatc
      121 agcgctgccg ctggtagaga accgtgccgt ccgcctgact cacattgtag agcgacgtgc
      181 agcgcgggtt cttcagctcg cggcagacca gatccaccgc caggacgtat ttggccgcgc
      241 tggtcgtggc gatgcagaga taccggccct gcacgcgcgt cagggcgagc ttggcgctgg
      301 cgagctgccc gtattgcgga acgatgccga agtttttcgc gctgccgagc gccatggtgc
      361 aaatctgcgc ctcggcgttg agaagattcg tgatgcggtt cacaagcgcc gtgtgctcct
      421 gccgcggttt gatgatttcg ggaacgatca cgccgaacca gccccctgcc gcgatggcga
      481 tgacgaatcc gccgatgacg acgattcggg cgatcagcca ttcgcgccgc aaggcgcgcc
      541 tggtctcggc aaggtcaggt gacgacattc gctgcggtcc gttcgcgggt catttcatgg
      601 tcgggatgct gaaatcggga cgctcgcgca agccgccccg gggccagcgc tgggtgacgg
      661 tcttgatgcg ggtgaagaat cgcacgccct ccggtccatg ctggttcacg tcgccgaatg
      721 ccgaccgctt ccagccgccg aaactgtgat aggcgagcgg caccggaatg ggaatgttga
      781 ttccgatcat tccgactttc accctgctcg cgaactcgcg ggcgcaatcg ccgtcgcggg
      841 tgaagatcgc gactccgttg ccgtattggt gattggaggg aagcgcgagc gccgcatcga
      901 aatcgggcgc ccgcgccacc tgcagtaccg gtccgaaaat ctcgtcccga taggtcttca
      961 tccccggcgt gacccgatcg aacagcgaac ccccgagaaa gaaaccgttc tcgtaaccct
     1021 ggaggctgaa gccccgtccg tcgaccacga ggtctgcgcc ctccttcacg ccaagctcga
     1081 tgtaggagag cactttctgg cgatgctctg cggtcaccat gggtccataa tccgcatcgg
     1141 gatcggtggg cggcgcgacc ttgagacatt cgacgcgcgg cttcaggcgc tccacgagcg
     1201 cgtcggcgag tttgtcggtg accgggacgg cgaccgaaat cgccatgcag cgttcgccgg
     1261 ccgaaccgta acccgcaccg atcaattcgt tcgtcacctg gtcgagatcg gcgtccggca
     1321 tgatgatggc gtgattcttc gcgccgccca tcgcctgcac gcgcttcccg cgcgccgttc
     1381 cgtgtgagta cacatattcg gcgacggccg acgaaccgac gaaactcacc gcctggagat
     1441 cggggtcttc gagaatggcg tcgacggctt ccttgtcgcc gtgcacgaca ttgagcacgc
     1501 ctgccggcgc accggcctcc atgaacagct ccgccaggcg cagcggaaca ctcggatcct
     1561 tttcgctcgg cttgcagatg aaggtgttgc cgcaggcgat ggcgacgccg aacatccaca
     1621 tcgggatcat ggcggggaag ttgaacgggg caatgccggc aacgacgccc aaaggctgcc
     1681 gcatcgagta gatgtcgatg ccgggacccg cgccctcggt gtactcgccc ttcatgagat
     1741 gaggaattcc gcacgcgaac tcgatgactt cgagcccgcg ctgaatgtcg ccgcgcgaat
     1801 ccgcgatgac cttgccgtgc tcgctcgaca gcagatgcgc gagctcgtcg atgttccttt
     1861 cgacgagcgc cttgaagtcg aacatgacgc gggcgcgccg ttggggattg acccccgacc
     1921 attcgcggaa tgccttcgcg gcagtctcca ccgcggcgcg cacttcgctg cggctcgcga
     1981 gaggcaccct cgcctgcacc tcgccggtgg cgggattgta gatgtcgcca aagcggcccg
     2041 agcggccttt ggcaaaccgt cccccgatga aatgcgcgag ctctctcatg gaaacctggc
     2101 gtctctgaaa ccgtgcggtt gggctagata gcgacggcaa ggaaacgcgg caagcatgga
     2161 cgccgcgcaa aaaaagaggc cgcacccgac ggatgcggcc ccaggggagt cgaattgcag
     2221 cccgatcagc ggttcagcgc aagcttccgc ccatcgctga ggcgcgagcc ttcataggcg
     2281 ccgtccttcg atttcagcca ggcgaccata tcgcccgagg gagagaagag ctgcagtccg
     2341 gtaggcgtgg ccttccagcg gccgatcgcg ttcagcccgc cggcgcaatc cggggacggt
     2401 gttgcgatcc ccgcgctgtc ggccgtctgg tccggcgcga gcgtaaggat gcagggtgca
     2461 tcgttttcgc cgacactgag cttccagaca cccgctatgg tctggtctgc atgggcgagg
     2521 gcaaccgtcg aaaatgccag aacggcccct atggccagag cgctgagcct cgtcatatga
     2581 atatctcctt catcgtttag cgttcagaca atagtaatca cgatggcagt tgagaagagc
     2641 gctctgcgca tgcccttgat gaatttttgt ggggactgaa cggtacccgg agacaattcc
     2701 attgcaaatt caatgggttg aaatggagcc tcggaaaaca tgctaaaaaa gagcgacgag
     2761 tttaccaaat tggagatatc ggccggctgc ctcgtcgcca tccggaatcg cccgggcgat
     2821 aaccccatcg gcataagata aggtgatacg accgttgggt gcctgctttt cctcccccac
     2881 gatggggaag gaaaacgccg catggtctgg ctgaggcgcg gtccactttg ccctcgcacg
     2941 cgctggcgaa tcaggcacaa tacgaggcgc tgttggcgtt agtgaggtgg caacatgcgt
     3001 tcgaggattt ggacgatttg ccgccgggcg ctctggccgg atgccggccg cccatccgcg
     3061 aacccgcgtc gcaccctcct ccgaacgata tgacctctgt ctcccatgtt gccggcgacg
     3121 ctgcgccggg aggaatctgg cggtgggcgg cactggcgct cttcctgatc gcgctcgcag
     3181 cggccggctt ggcggtggca accgccggcg cgtccgggtg gtcgggggtg gcgctgctgg
     3241 ccggcattgg atcgatcgcc ggattgtttc ttgccgtcat ctggccgcgc gccaacgttg
     3301 ccgccgccga tgcccgccgc gttgccgaag ccgcggcgaa ggcgaatgtc gcctgggcga
     3361 tcacgaatcg cgacggcgtg gtgatccatt ccaacgaagc ctaccggcgc ctcgccggcg
     3421 cccgcgaacg tgaagcgccg ccgccgcccg aactggcgct gcagggcgag tccgcggcct
     3481 cggtgctgta ccgcctgagc cgcaaggcgc gcgccggaca gtcccatgaa gaaatattgc
     3541 aggccgcgtc cggactcgaa cttctcgttg ccgtgcgtcc cttgtccgat ggccgcgccg
     3601 cctggtggtt cacgccacgc gtcgcggcgc gggaggccgc ttccattgcg acggcgccgg
     3661 ctggcgccgg atccgcgtcc ggcctttcag gcggcgccgc gatgttccgc gacgcgccga
     3721 tcggcatcgc cattgcggag cccgatggca cgctggtcga aacgaacgcg ctctttcagc
     3781 aattcttcgg tgcagcgggc gatctcggcg ggcgcgggct cgccgatctc gcggcgcccc
     3841 ccgacagagc cgatatcgcc gaaacttttg tgcgcgccgc ggcaggagag agcgctgcgt
     3901 tcggcctgcg cgccgccagc ggcgtcgaaa gcctg
//
LOCUS       DASZMC010000279         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_406692,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000279 DASZMC010000000
VERSION     DASZMC010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406692"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1982)
                     /locus_tag="VG274_05720"
     CDS             complement(<1..1982)
                     /locus_tag="VG274_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWE06185.1"
                     /translation="MRLRGWFAFGIGIAAMIAVWGHDGEAREQMTANDPWLWLEDVHG
                     ARPLAWVRQENARTLAVLAADPDYRRDYDAALSILDAEDRIPFGDIDRQYVFNFWQDR
                     KNPKGVWRRTTIADYETASPHWETLLDVDNLAEAEHENWVFKGATCSPALLHCLVSLS
                     RGGGDARVVREFDLAAKKFSANGFSLAEAKSYAAWLDEDTILFGTDFGKDSLTSSGYP
                     RIVKVWKRGEPIASAREAFAGKPEDVSVSPTALRAPTGSLGVIIRSTTFFETEYYLPA
                     ASGEWKRLPVPLSAVLQGMTGGQLIFTLRGDWATPEGRKIEKGTLLAIPVKPFLAGGK
                     LPPPVVLYAPRARISVEQVSCGRDAVYAAVYDNVVGSIHAFGLDPGKNAWRETKLDLP
                     GGGATHIVTTNDYGPEAQFTFESFLTPTTLFADEGIDHPHAIKSIPARFDASGLSMTQ
                     HEATSRDGTKIPYFVVAPSGAQGPRPTILYGYGGFEISQTPWYWASAGKVWLTKGGVI
                     AVANIRGGGEFGPAWHDAAVKIHRQRAFDDFAAVAADLSARRITTPRQLGIMGGSNGG
                     LLVSTVMAQHPELFGAVVCQVPLTDMLRYARIGAGASWIAEYGDPENPEMRGAILKYS
                     PYQNVRPGVKYPPVLFVTATSDDRVTPVHARKMAAK"
     gene            2083..2922
                     /gene="dapF"
                     /locus_tag="VG274_05725"
     CDS             2083..2922
                     /gene="dapF"
                     /locus_tag="VG274_05725"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWE06186.1"
                     /translation="MTPFYKMQGLGNDFVVFDARNHGPALDEAAARAIADRRLGVGCD
                     QVIVIEQPRARGDAMMRIFNADGDEVEACGNAARCVARLLMEEKDETTVRIDTLGGLV
                     VCSDAGAGAVTADMGAPRLQWHDIPLADSIDTRAFALPVDGELLTATAVSMGNPHCVI
                     FVDNAERTPVADLGARIEKHPLFPERTNVEFVSVPEPGRLRMRVWERGAGITRACGTG
                     ACAAAVAAHRRELGPRAWEVVLDGGMLSIDWREADDHVLMTGGATLTFAGQIDLAMLR
                     ARQ"
     gene            2919..4175
                     /gene="mtaB"
                     /locus_tag="VG274_05730"
     CDS             2919..4175
                     /gene="mtaB"
                     /locus_tag="VG274_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HWE06187.1"
                     /translation="MSRPEVITFGCRLNAYESEAIRARAAEAGLHDVTIFNTCAVTAE
                     AVRQSRQAIRRKRRGNAQTKIIVTGCAAQTEPATYAAMPEVDLVLGNREKLETASYAD
                     FGVAAAERVRVNDIMSVKETAAQFVDSFHGRARAFLQIQNGCDHRCTFCIIPFGRGNS
                     RSVPMGAVVEEARRLCAHGYPEIVLTGVELTAYGADLPGSPSFGTLVRKLLQMMPELP
                     RLRLSSIDSIEADPELMRAIAEEERLMPHFHLSAQSGDDLILKRMKRRHSRRDTIAFC
                     EQVRRLRPDAAFGADLIAGFPTESEEMFGNTLKLVDDAGFTFLHVFPYSPRHGTPAAR
                     MPQLARATVRERASRLRAHGAHKLAIRLNDLVGGEQSVLVEEAGRGRTPCFVSARFSQ
                     QAEPGTLMRVRIISAAADHVLAEALA"
     gene            4172..5110
                     /gene="ftsY"
                     /locus_tag="VG274_05735"
     CDS             4172..5110
                     /gene="ftsY"
                     /locus_tag="VG274_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWE06188.1"
                     /translation="MNIEASAAPGLLGRLKTGLSRSANTLAAGLTGLFTKKKLDAETI
                     VELEEALIRADIGLIRASNIAAAVARGRYDSEISEGELRSVLAAEIAAHLKSVEVPFE
                     IDRGHKPFVVLIVGVNGTGKTTTIGKLARKLTAKRQKVVLAAGDTFRAAAIEQLQIWG
                     TRTGAEVFARGAGADAAGLAFDALAHARANGADVLLVDTAGRLQNKAGLMAELTKIVR
                     VLKKLDPDAPHSVLLVLDATTGQNAISQVEAFKATVPLTGLIMTKLDGTAKGGILVAL
                     ADRFELPVPYIGVGEGEDDLQAFQAAAFARALTGAS"
     gene            5107..5676
                     /locus_tag="VG274_05740"
     CDS             5107..5676
                     /locus_tag="VG274_05740"
                     /inference="COORDINATES: protein motif:HMM:NF001323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HWE06189.1"
                     /translation="MSRAGKSSHLVRIALDLGPLVIFFGSYQYFGIFTATACFMAAVL
                     FALGAGYIRDRRLPPMPLFTAVIVLIFGGLTLYLRNDVFIKMKPTVLYALFGFLLVGG
                     LAFNQLLIKHVFSHAFVLSDKGWRGLTWRWAVFFLGLAMLNEIVWRNFSTSTWVYFKV
                     WAVIPLIFLFALAQTPLILKHEIRADAQE"
     gene            5962..6471
                     /locus_tag="VG274_05745"
     CDS             5962..6471
                     /locus_tag="VG274_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWE06190.1"
                     /translation="MSKGQRKANGSVELAETPSHLMRRCHQFYGDLYARESGDRNLTK
                     QQYLVLCALEQHDGVSQTSLVETTGIDRSTLAEMIRRMLERGVVSRKRADEDARANTV
                     VITQAGKRVLRAARLAAEKAERALLEPLPAPERLRFVKLLAMIAAAADQQPAIGSGKA
                     HRKPASRRA"
BASE COUNT         1236 a         2091 c         2071 g         1221 t
ORIGIN      
        1 atcttcgccg ccatcttgcg ggcatggacg ggcgtgacgc gatcatccga cgtcgcggtc
       61 acgaaaagca ccggcgggta tttcacgccg ggccgtacgt tctgataggg cgagtatttg
      121 aggatggccc cgcgcatctc cggattttcc ggatctccat attcggcgat ccatgacgcc
      181 cccgcgccga tcctggcgta gcggagcatg tcggtcagcg gcacctggca gacgacggcg
      241 ccgaacagtt cgggatgctg cgccatgacc gtgctgacca ggagcccgcc attcgagccg
      301 cccatgatcc cgagttggcg cggcgtggtg atccggcgcg ccgagagatc ggctgcgacc
      361 gccgcaaaat cgtcgaaagc gcgctgacga tgaatcttga ccgccgcatc atgccaggcc
      421 ggcccgaact cgccgccccc gcggatattg gcgacggcga tgaccccgcc ctttgttagc
      481 caaacctttc ccgcactcgc ccaataccag ggcgtctgcg aaatttcgaa cccgccatag
      541 ccgtagagga tcgtgggtcg tggtccttgg gcgcccgatg gcgccaccac gaaataggga
      601 atcttcgtgc cgtccctcga cgtcgcctca tgttgtgtca tggagagccc cgacgcgtcg
      661 aagcgcgcgg gaatcgattt gatggcgtgc gggtgatcga tcccctcgtc ggcaaagagt
      721 gtcgtgggcg taagaaagct ctcgaacgtg aactgggctt cagggccgta atcgttggtc
      781 gtcacgatat gggtggctcc gccgcccggc aggtcgagct ttgtctcgcg ccaggcgttc
      841 tttcctggat cgagtccgaa agcgtgaata ctgcccacca cattgtcgta aacggccgcg
      901 tacaccgcgt cgcgcccgca cgagacttgt tcgaccgaga tgcgtgcacg aggtgcgtag
      961 agaacgaccg gcggcggaag cttgccgccg gccaggaacg gtttcacggg aattgcgagg
     1021 agtgtgccct tttcgatttt ccgcccctcg ggcgtcgccc agtccccacg caaggtgaaa
     1081 atgagctggc cgccggtcat gccctgcagc acagccgaaa gcggcaccgg cagccttttc
     1141 cattcgccgc tcgccgccgg aaggtaatac tcagtctcga aaaacgtcgt ggagcgaatg
     1201 atgacgccga gagagcccgt gggcgcgcgc aacgcggtgg gtgaaaccga aacatcttcg
     1261 ggctttcccg cgaacgcctc tcttgcgctc gcgatcggct cgccgcgctt ccagactttc
     1321 acgattcgcg gataacccga actcgtgagc gagtccttgc cgaaatccgt gccgaacaga
     1381 atcgtgtctt catcgagcca tgcggcgtag gacttcgctt ccgcgagcga aaagccgttc
     1441 gcggagaatt tcttcgcggc gagatcgaat tcgcgcacca cccttgcgtc accgccgccg
     1501 cgggagagcg agacaaggca gtgcaggagc gccggcgaac aggtggcgcc cttgaagacc
     1561 cagttctcat gttcggcctc cgcgagatta tcgacgtcga gaagcgtctc ccaatgcgga
     1621 cttgcagttt cgtaatcggc gattgtggtt cgccgccaaa cgcctttcgg atttttccga
     1681 tcctgccaga aattgaaaac atattgccgg tcgatgtcgc cgaaggggat gcggtcttcg
     1741 gcgtcgagaa tggagagcgc ggcatcgtaa tcgcgccggt agtcggggtc cgcggccagc
     1801 actgcgagcg ttcgtgcgtt ctcctgcctc acccaggcaa gcgggcgcgc gccgtgcacg
     1861 tcttcaagcc agagccacgg atcgttggcc gtcatctgct cccgtgcctc gccgtcatga
     1921 ccccacacgg caatcatggc ggctattcca attccgaatg cgaaccagcc gcgcagtcgc
     1981 atgcctgcct ctctctcggg ccgaaacggt attgcgaaca ccgcggcaaa acgtcaatgc
     2041 gctggctggc tctccaaact tccacagcct ataagccccg ccatgacacc gttctacaaa
     2101 atgcagggtc tcgggaacga ctttgtcgtt ttcgacgcgc gcaatcatgg gcctgcgctg
     2161 gacgaagcgg cggcgcgcgc cattgcggac cggcggctcg gcgtgggttg cgatcaggtg
     2221 atcgtgatcg agcaaccccg cgccaggggc gacgccatga tgcggatttt caacgccgac
     2281 ggcgacgagg tcgaagcttg cggcaatgcc gcgcgctgcg tggcacgcct cctgatggag
     2341 gaaaaggacg aaaccacggt gcgcatcgac acgctgggcg gactggtcgt atgcagcgac
     2401 gcgggggcgg gcgcggtgac cgccgatatg ggcgctccga ggttgcaatg gcacgacata
     2461 ccgctcgcag acagcatcga cacgcgcgcc ttcgcgctgc cggtggacgg cgaactcctg
     2521 acggcgacgg ctgtatccat gggcaatccc cattgcgtca tcttcgttga caacgcggag
     2581 cgcacgccgg tcgcagatct gggcgccagg atcgaaaaac atcccctgtt tccggagcgg
     2641 accaacgtcg aatttgtaag cgtgcctgag cccggccgtt tgcgcatgcg ggtctgggag
     2701 cgcggggcag gcatcacgcg ggcgtgcgga acgggggcct gcgcggctgc agtcgccgcg
     2761 catcggcgcg aacttggccc gcgcgcctgg gaggtggtac tcgatggcgg catgctgagc
     2821 atcgattggc gcgaagctga cgatcacgtc ctgatgaccg gcggcgcaac cctgaccttc
     2881 gccgggcaga tcgacctcgc aatgttgcgt gcgcgccaat gagccgcccg gaggtgatca
     2941 ccttcggctg ccggctgaac gcctacgaat ccgaagccat cagggcgcgg gccgccgagg
     3001 ccggattgca tgacgtcacg atcttcaaca cctgtgcggt caccgccgag gccgtgcgac
     3061 agtcgcgcca ggcgatccgc aggaaacggc gcggcaacgc acagacgaaa atcatcgtca
     3121 cgggatgcgc cgcccagacg gaacccgcga cctatgcggc catgccggaa gtggatctgg
     3181 tgctcggcaa tcgcgagaag ctcgaaactg cgagctacgc ggatttcggc gtcgccgcag
     3241 ccgaacgggt gcgagtcaac gacatcatgt cggtcaagga aaccgcggcg caatttgtgg
     3301 attcgtttca cggccgcgcc cgcgccttcc tgcaaattca aaatggctgc gaccaccgct
     3361 gcaccttttg catcatccct ttcgggcggg gaaattcgcg cagcgtgccg atgggcgcag
     3421 ttgtcgagga agcgcggcgt ctctgcgcgc acggctatcc ggaaatcgtg ctcaccggcg
     3481 tggagctgac cgcctatggc gccgatcttc ccggctcgcc ttcgttcggc acgttggtcc
     3541 gcaagctttt gcagatgatg cctgaactcc cgcggctgcg tctctcatcg atcgattcca
     3601 tagaggccga ccccgaactc atgcgcgcga tcgccgagga agaacggctg atgccgcatt
     3661 tccatctctc ggcgcaatcg ggcgacgacc tgatcctgaa gcgcatgaaa aggcggcatt
     3721 cgcggcgcga cacgattgcc ttctgcgagc aagtgcggcg attgcggccc gatgccgcct
     3781 tcggggcgga tctgattgcc ggctttccca ccgaaagtga ggagatgttc gggaacacgc
     3841 taaagcttgt ggacgacgcc ggctttacat tcctgcacgt gtttccctac agccccagac
     3901 acggaacgcc cgcggcgcga atgccccagc tcgcacgggc gacggtcagg gaacgcgcca
     3961 gccgcctgcg cgcgcatggc gcacacaaac ttgccatccg gctgaatgat ctcgtgggcg
     4021 gcgagcaatc ggtgctggtt gaagaggccg gccgcggccg aaccccctgc ttcgtctcgg
     4081 cgcggtttag tcagcaggct gagccgggaa cattgatgcg cgtgcgcatc atttctgctg
     4141 ccgccgatca cgtcttggcg gaagcactcg catgaacatc gaagcttccg cggcgccggg
     4201 ccttctggga cggctcaaga cgggactttc gcgcagcgcc aacacgctgg cggctggcct
     4261 caccgggctg ttcaccaaga aaaaactgga cgccgaaacc atagtcgaac tcgaagaagc
     4321 gttgattcgt gcagacatcg gcctcatccg cgcttccaat atcgccgccg ccgtcgcgag
     4381 aggccgctac gattcagaaa tttcggaagg cgagctgcgc tcggtgctcg ccgctgaaat
     4441 tgctgctcat ctcaaatccg tggaagtccc ctttgaaatc gatcgtggcc acaagccgtt
     4501 cgtcgtgctg atcgtgggcg tgaacgggac gggaaagacg acaacgatcg ggaagctggc
     4561 gcggaaattg acggccaagc gccagaaagt cgtgctcgcg gcaggcgata cattccgtgc
     4621 tgcggcgatc gagcagctgc agatttgggg cacgcgcacg ggcgccgagg tcttcgcgcg
     4681 cggcgccggc gccgacgcgg cgggcctcgc tttcgacgca ctcgcccatg cgcgcgcaaa
     4741 cggcgccgac gtgctgctcg tcgatacggc gggccgtctc cagaacaagg caggcctgat
     4801 ggccgaactg acgaaaatcg tacgcgtact gaaaaagctc gaccccgatg cgcctcattc
     4861 ggtcctgctg gttctcgacg ctacgactgg gcagaatgcg atttcgcagg tcgaagcgtt
     4921 caaggccacg gtgcctttga ccgggctgat catgaccaaa ctcgacggca ccgcgaaagg
     4981 tggaattctt gtcgccctcg ccgaccggtt cgaattgccc gtgccctata ttggcgtggg
     5041 agaaggcgaa gatgatctcc aggcttttca ggctgccgct ttcgccagag cgctgacggg
     5101 ggcatcatga gccgtgcggg caaatcatcg catctggtcc ggatcgcgct cgatctggga
     5161 ccgctcgtca tattcttcgg gtcgtaccag tatttcggca tattcaccgc gaccgcgtgt
     5221 ttcatggcgg ccgttctgtt cgcgctggga gcaggataca ttcgcgatcg gcgcctgccg
     5281 cccatgcctc tctttaccgc cgtcatcgtg ctgatcttcg gcggactcac gctctatctg
     5341 cgcaacgatg ttttcatcaa aatgaaaccc accgtgctct acgcgttgtt cgggttcctg
     5401 ctggttggcg ggctcgcttt caaccagctg ctcatcaagc atgtattctc ccatgccttt
     5461 gttctcagcg acaaaggctg gcgcggcctg acgtggcgtt gggcagtgtt ttttcttggc
     5521 ctggcgatgc ttaacgaaat cgtatggcga aatttttcca cttcgacctg ggtctatttc
     5581 aaggtctggg ccgtgatccc gctgattttc ctcttcgcac ttgcacagac gcctcttatc
     5641 cttaagcacg agatccgcgc ggatgctcag gaatagccag cttcgcttgc aaaccgattt
     5701 ttccaactct tggtcgcgcg aaacgggcgg aagaatcttg cggcgcggac gcggcggccg
     5761 gcgcagcgtc gaccggccgg ctttgcgaca acgcgacacg cgcaaataat tgcgcaatgg
     5821 aacgcttgtc gtgcgcattc ttcgcgacta agcttcgcgc atctccgcgg aggaggcgac
     5881 gtccacgaat gcttcgcccg gcgttgtgcg ggtcggtctc cgcaaagagc aaatgcattt
     5941 tcggtcttgg gggcttcacg aatgtcgaag ggccagcgaa aggcaaacgg aagcgttgag
     6001 cttgcggaaa cgccgtcgca tctcatgcga cgatgtcatc agttttacgg cgacctttat
     6061 gcgcgcgagt ccggcgacag aaatctgacg aagcagcaat acctcgttct ctgtgcactc
     6121 gagcagcacg atggcgtcag ccagacgtcg ctcgtggaga cgacgggcat tgatcgttcg
     6181 actcttgcgg agatgatacg gcgcatgctc gagcgcggcg tcgtctcgcg caagcgcgcc
     6241 gatgaagacg cccgggcgaa taccgtcgtg atcacccagg ccggcaagcg cgtgctaagg
     6301 gctgcgcgcc tcgccgcaga aaaggccgaa cgagccctgc tggagccgct cccggcgccg
     6361 gagcgcctcc gcttcgtcaa gcttctcgca atgatagccg cggccgccga ccaacagccc
     6421 gcaatcggca gcggaaaagc gcatcgaaag cccgcctcaa gacgggcgta aacaacgcct
     6481 agctcgccgc gcgcgcacgc tcgatcgcgg gaagaatgac gcggtccagc gcgtcaccga
     6541 cgatggggcc ggcatagcgc aggcgcacca cgcccgctct gtccacgacg aatgtttccg
     6601 gcacgccgta gacacccca
//
LOCUS       DASZMC010000280         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3673497,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000280 DASZMC010000000
VERSION     DASZMC010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3673497"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VG274_05750"
     CDS             <1..903
                     /locus_tag="VG274_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWE06191.1"
                     /translation="MFAIITPALITGAFANRVTFKAYMAFLTLWLLFVYFPFVHMLWG
                     GGLLQRWGVLDFAGGIVVHNTAGLAALASVLYVGRRHVVDTGAHSVPLVALGTGLLWF
                     GWYGFNAGSEFQVDSVTAVAFLNTDVAASFAAITWMAVGWWLEKRPTFVGLLTGAVAG
                     LATITPAAGYVSPLSAVIIGIVSGGVCYFAVALKNRLRWDDALDVWGVHGVGGALGIV
                     LLGVFATTAFNPHGADGLMTGNSAFFGKQVAAIALSSVWAFGFTYAMLWLIDRVTPVK
                     VDQAAEVSGLDSALHGETAYLGET"
     gene            complement(921..1337)
                     /gene="dksA"
                     /locus_tag="VG274_05755"
     CDS             complement(921..1337)
                     /gene="dksA"
                     /locus_tag="VG274_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HWE06192.1"
                     /translation="MGIELPPGYKPSESESFMNERQREYFRRKLLRWKDEILRESRGT
                     IQNLQAETVSNADLADRAATESERQLELRTRDRQRKLIAKIDAAIRRLEDGSYGFCEE
                     TGEPISLKRLDARPIATLSIEAQERHERRERVYRDD"
     gene            complement(1454..>1859)
                     /locus_tag="VG274_05760"
     CDS             complement(1454..>1859)
                     /locus_tag="VG274_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWE06193.1"
                     /translation="SRVIGRRQGINSLAVEEVAVSKFREARARIAEEGEKLAGRIPAG
                     AHIIALDARGKGMTSEAFAEMLGAMRDAGARDLAFLIGGPDGLELGPGVKPGRSLAFG
                     PQTWPHLLVRAMLAEQIYRALTILGGHPYHRA"
BASE COUNT          274 a          576 c          618 g          391 t
ORIGIN      
        1 atgttcgcga tcatcacgcc ggcgctgatc acgggagcct tcgccaatcg ggtgacgttc
       61 aaagcgtata tggcttttct caccttgtgg ctgctgttcg tctatttccc cttcgtgcac
      121 atgctgtggg gcggcggcct gttgcaacgc tggggcgtgc tcgattttgc cggcggcatc
      181 gtggtgcaca atacggcggg actcgcagcc ctcgcctccg ttctctatgt gggccgccgc
      241 catgttgttg atacgggcgc gcatagcgtt ccgctcgtcg cgctgggcac gggactgctg
      301 tggttcggct ggtatggttt caatgcgggc agcgagttcc aggtcgactc cgtcaccgcc
      361 gtcgcgttcc tgaacaccga cgtcgcggct tcgtttgcgg cgatcacgtg gatggctgtc
      421 ggctggtggc tcgagaaacg gcccaccttc gtcggcctcc tgaccggcgc cgttgcgggc
      481 ctcgccacga tcacgcccgc agcgggctat gtctcaccct tgtcggcagt gatcatcgga
      541 atcgtatcgg gcggcgtctg ctattttgcg gtggcgttga agaaccggct gcgttgggac
      601 gacgcgctcg acgtgtgggg cgtgcacggt gttggcggcg cgctcggcat cgttctcctt
      661 ggcgtctttg cgaccaccgc tttcaatccg catggcgcag acggtctcat gaccggcaat
      721 tctgcctttt tcggcaagca ggtcgccgcg atcgcgctgt cgtccgtctg ggcctttgga
      781 ttcacctacg cgatgctgtg gctgatcgac agggttacgc cggtgaaagt cgaccaggcc
      841 gcggaggtca gcggcctcga ttctgcgcta catggcgaaa ccgcctatct cggcgagacg
      901 tgaagcccaa agcgcgtgcg tcaatcgtcg cggtaaaccc gctcacggcg ctcgtggcgt
      961 tcctgtgcct cgatggaaag ggtggcgatc gggcgcgcgt cgaggcgctt gagcgaaatc
     1021 ggctctccgg tttcttcgca aaatccgtac gagccgtctt cgagacgacg gatcgccgcg
     1081 tcgatcttgg cgatcagctt gcgctgccgg tcgcgagtgc gcagttcgag ctggcgttcg
     1141 gattcggtgg cggcgcgatc cgccagatcg gcatttgata ctgtctcggc ctgaaggttc
     1201 tggatcgtgc cgcggctctc gcggaggatc tcgtctttcc agcgcagcaa tttgcggcgg
     1261 aaatactcgc gctgccgctc gttcatgaac gactcgctct ccgacggctt atagccgggc
     1321 ggaagttcga tacccatgga tactcccctg aaagcgagtg gcccatcgac gagcgggcgg
     1381 gtttatacag gccgggttcc cgggcagcaa atggaattcg cgaacgtctt tggacgattg
     1441 gcgggtcgcg gagtcaggcg cggtgatagg gatggccgcc gagaatggtc agggcgcgat
     1501 agatctgctc cgccagcatt gcccgcacca gaagatgagg ccaggtttgc ggaccgaagg
     1561 cgaggcttcg tcccggcttc actcccggcc cgagctcgag cccgtccggg ccgccgatca
     1621 gaaatgcgag atcgcgtgcg ccggcgtcgc gcatcgcgcc cagcatctcg gcgaaggctt
     1681 cgctggtcat gcccttgccg cgcgcgtcga gcgcaatgat gtgggcgccg gcgggaatgc
     1741 gccccgcgag cttctcgcct tcctcggcga tgcgggcgcg cgcttccctg aacttcgaaa
     1801 ccgcgacttc ttcgacggcg agcgaattga tgccctgacg gcggccgatc acccgcgac
//
LOCUS       DASZMC010000281         5357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4512083,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000281 DASZMC010000000
VERSION     DASZMC010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4512083"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VG274_05765"
     CDS             <1..216
                     /locus_tag="VG274_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06194.1"
                     /translation="IMLNGGYFDLATPYYEGWYEMHHLQIPQNLQANISYRYYDSGHM
                     VYAHGPSLKELHDNVAAFIRQTDNVQK"
     gene            581..1516
                     /locus_tag="VG274_05770"
     CDS             581..1516
                     /locus_tag="VG274_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWE06195.1"
                     /translation="MPGSQDYAADPRNQTVLVWLNGTLVPRDEAKVSLFDAGFVLGDG
                     VWEGLRLHKGGLLFLDVHLDRLYAGARAIALDIGLARTDIVAALRATLSANAMQHGVH
                     IRLMVTRGEKKTPNQDPRNALGRPTVAIVAEYKEPGATVRAQGLNLFTSTIRCTPADM
                     FDMRLNSHSRLSLIIALNQAIAAGADEALMLDPNGFVSSCNATNFFFVSGHEVCTSTG
                     AYCFNGVTRANMIALCQRHDIPFRLGDFTLHDVYAAGEAFVTGTFGGVIPVRAIDGRA
                     LPVAPPDGTTRRIEGLYERLKDEEAAADIGRATPQ"
     gene            1513..2313
                     /locus_tag="VG274_05775"
     CDS             1513..2313
                     /locus_tag="VG274_05775"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06196.1"
                     /translation="MSEEPIRIAMWSGPRNISTAMMRAFENREDAAVIDEPFYAAYLK
                     LTGTEHPLRETVLEAHESDWRKIIEILQGPVPGGRHVFYQKHMTHHMAPAIPRDWMLS
                     CRNAFLIRSPEAVLASYSAKRSQFLPADIGFEIQREMFEREADRLGAAPPVIDADDVL
                     RGPRGILHALCARLGIPFSDRMLRWPAGRRPTDGAWAPAWYDAVEKSTGFGSPRPLPA
                     EPLPDHLKPVADASRPHYALLARFKLRSDAVGYAGEVPWNPESRETPE"
     gene            complement(2411..2704)
                     /locus_tag="VG274_05780"
     CDS             complement(2411..2704)
                     /locus_tag="VG274_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWE06197.1"
                     /translation="MSKTYYVYILASARNGTLYIGVTNDLARRCYEHREGLMPGFTRR
                     YDVNRLVHVEPFSDIGFAIQREKRLKKWNRRWKLELIEATNPQWLDLYQTLNG"
     gene            complement(2935..4074)
                     /locus_tag="VG274_05785"
     CDS             complement(2935..4074)
                     /locus_tag="VG274_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HWE06198.1"
                     /translation="MNDKQSLANHPDTIAAQALGWVDRETRAVVLPLHLSSTFERDAD
                     NLYRSGRNYARADNPSFDQPEAVLAKLENGAAAMLFASGMAAATCAFQTLSPGDHVVA
                     PKVMYWALRHWLANQSHLGAEFIDMSDSGALARALIPGKTKMVWVETPANPLWDITDI
                     EAAVALAHRAGARVAVDSTVATPVFTRPLELGADLVMHSATKYLNGHSDVVAGALVTR
                     EIDAAWQKLKSLRVHLGGIPGSFESYLLTRGMRTLFPRVQLAAANARDLADRLVAHPK
                     ISHVLYPGLPDFRGHAIAARQMKGGFGAMLSIRVAGGEGAAIETAGRLRVWKRATSLG
                     GVESLVEHRASIEGPDTPCPRDLLRLSVGIENIEDLHADLAEALM"
     gene            complement(4168..5187)
                     /locus_tag="VG274_05790"
     CDS             complement(4168..5187)
                     /locus_tag="VG274_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWE06199.1"
                     /translation="MSLWPLVSRPYRFGIRFRPPEPRAESRRRRVDSFHWENVGVDPR
                     ARLRCGTFAASDGAEVPYRLWLARQPRAAVLLLHGAFDYAAAFEEIGPRLARRGFTSL
                     AFDQRGFGATASRGAWTGPARMAADMIEAAGFVQGRVPANAPVFAIGESLGGAVAIHA
                     AADGMAPRLKGLVLAAPGALASPFRQRLLAWFAASLRAMGGDAELVFERLSGWELTPA
                     AAIRLLGDPLVMRRIPPEMLCGMASLALASVSEAKRVNVPVLTMVGARDELVRQSCIR
                     QLFDNLDGPKSWRVVPDAPHLLLHWRRSAEVLREATRWMCAQLSPASPEAVGAKTPGD
                     TGLAA"
     gene            complement(5184..>5357)
                     /locus_tag="VG274_05795"
     CDS             complement(5184..>5357)
                     /locus_tag="VG274_05795"
                     /inference="COORDINATES: protein motif:HMM:NF010229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HWE06200.1"
                     /translation="RLAVLFLILGLVAAVLGFGGIAGLSFAIAKLLFVIFLVLCLVFL
                     VLGLRVGRGIARR"
BASE COUNT         1039 a         1689 c         1682 g          947 t
ORIGIN      
        1 atcatgctca atggcggata tttcgacctg gcgacgccct actacgaggg ctggtacgaa
       61 atgcaccatt tgcagatccc gcagaacctg caggcgaaca tctcctaccg gtattacgat
      121 tcaggccaca tggtctacgc gcacgggccg tcgctcaagg agctgcacga caatgttgcg
      181 gccttcatcc ggcagacaga caatgttcaa aaataagcca gccagccgga cttagagcgc
      241 tgttcacccg gggaccggct caggatgagg ccctcatggt gagcttgtcg aaccatgagg
      301 gccggcggca cgctgcgcgc aatcactgcg cgatgaacgg gcgatgaatg ggagatgaac
      361 ggcgtcgcca taactcgttc aggagacttc gatcaatatc cgcgtcactg gtccggtttg
      421 ctgcaagccc ctccctgcag accgggcagc gaatagcggg agcccgcatc ggcgcagtcc
      481 gatgcgggct tccgtttttt cgccaccgcc gatcgggacg cggctttctc ccgcgcgcga
      541 atccgaggca atctccgccg gtccatggag tcttcgcgcg atgccgggta gccaggatta
      601 cgccgcagat ccccgcaacc agacggtcct cgtgtggctt aatggcacgc tcgtaccgcg
      661 cgacgaggca aaagtctcgc tgttcgatgc gggcttcgtt ctcggcgacg gcgtctggga
      721 ggggttgcgg ctgcacaagg gcgggctcct ttttctcgac gtccatctcg atcgtctgta
      781 tgcgggcgcg cgcgccatcg cgctcgatat cggactcgcg cgtacggata ttgtcgccgc
      841 gctgcgagcc acgctttccg ccaacgccat gcagcatggc gtccacatcc ggctcatggt
      901 cacgcggggc gagaagaaga cgccgaatca ggatccgcga aatgcgcttg gcagacccac
      961 cgtcgcgatc gtcgccgaat acaaggaacc gggcgccacc gtgcgcgcgc agggtctgaa
     1021 cctcttcaca tccaccatcc gctgcacgcc ggccgacatg ttcgacatgc ggctgaattc
     1081 gcacagccgg ctcagtctca tcatcgccct gaaccaggcg atcgccgctg gcgccgacga
     1141 agcgctgatg ctcgatccga acggatttgt ctcaagctgc aacgcgacaa atttcttctt
     1201 tgtttccgga cacgaggtgt gcacgtcgac aggcgcctac tgcttcaatg gcgtcacccg
     1261 cgccaacatg attgcgttgt gccagcgcca tgacattcct tttcgcctcg gcgatttcac
     1321 gctgcacgac gtctatgcgg ccggggaagc attcgtgacc ggcactttcg gcggcgtgat
     1381 ccccgtgcgc gccatcgacg gacgggcgct tcccgttgcg ccgcccgatg gaacgacgcg
     1441 ccgtatcgag gggctctatg agcgcctcaa agacgaagag gcagcggcag atatcgggcg
     1501 agccacgccg cagtgagcga ggagcccatc cgcattgcga tgtggtcggg gccgcgcaac
     1561 atctcgaccg ccatgatgcg cgccttcgag aaccgcgaag atgcggcggt gatcgacgag
     1621 ccgttttatg cggcatacct gaaactgacc ggaaccgaac atccgttgcg cgagacagtg
     1681 ctcgaagcgc acgaaagcga ttggcgaaaa attatcgaga tccttcaagg gccggtgccc
     1741 ggcggtcgcc acgtcttcta tcaaaagcac atgacccatc acatggcgcc tgcaattccg
     1801 cgcgactgga tgctgtcgtg ccgcaatgcc tttctcatac gttcgccgga agccgtgctt
     1861 gcgtcctaca gcgcaaagcg atcgcaattt cttccggcgg atatcggttt tgagattcag
     1921 cgcgaaatgt tcgagcgcga agccgatcga ctcggcgccg caccgccggt aatcgacgcg
     1981 gacgacgtgc ttcgcggtcc gcgcggaatt ctgcacgccc tttgcgccag gttgggcatt
     2041 ccgttctccg atcgcatgct tcggtggccg gccggccgac ggcctactga cggcgcctgg
     2101 gcgccggcct ggtacgacgc ggtcgaaaaa tcgactggct tcggttctcc gcgcccgctc
     2161 cccgccgaac ctttgccaga ccatctcaaa ccggtggccg acgcaagcag gccacactac
     2221 gccctgctgg cgcggttcaa gcttcgatcg gacgcagtgg gatacgcggg cgaagtgccc
     2281 tggaatcccg agagtcgtga gactcctgaa tgagttggtg cgtaatagag cgccgtctga
     2341 tcaaattcaa tctcactctc accttgtcat tcccgcagga gcgggaatcc agagccaacg
     2401 cacggcaccg tcagccgttg agcgtctgat acagatccag ccattgggga ttggtcgctt
     2461 caatcagttc gagtttccag cggcggttcc atttcttgag tcgtttctcg cgctggattg
     2521 caaagccaat gtcgctgaac ggctcgacgt gaacgaggcg attgacatcg taccttctgg
     2581 taaagccggg cataagcccc tcccggtgct catagcatcg ccgcgcgaga tcgttggtga
     2641 cgccgatgta gagggttccg tttctcgcgc ttgcaagtat gtacacatag taggtcttcg
     2701 acatttccgg tcatccgcaa tcgccagcaa ctgtgtgaat gaagacagat gttattgctt
     2761 gccgaatatg tcgcgtaggt tgcttttgag acagattgcg acggcgcttc gcgctaaagc
     2821 aacgcacgtc gcatgcccct ggagtcccgc ttcagcggga atgacatgtt tggtccaatt
     2881 tgaacagaac gggcactacg ctggcattct gccaaagcaa gtcgtgaatg taggctacat
     2941 gagcgcctcg gcgaggtcgg cgtggagatc ttcgatattc tcgatgccca ccgacagacg
     3001 caacagatcg cgcgggcagg gcgtatcggg gccctcgatg gacgcgcgat gttcgacgag
     3061 gctttcgacg ccgccaagag aggttgcacg cttccacacg cgcaggcggc ctgccgtttc
     3121 aatcgccgcg ccttcgccgc cggcaacgcg gatcgacagc atggcgccga agccgccctt
     3181 catctgacgc gccgcgatgg catggccccg aaaatcgggc aggccgggat aaagcacgtg
     3241 cgatatcttc gggtgcgcca cgagccggtc ggccagatcg cgcgcattgg cggcggcgag
     3301 ctgcacgcgg ggaaacagcg tgcgcatccc gcgcgtcagc aggtacgatt cgaaggaacc
     3361 cggaatgccg ccgagatgca cgcggagcga tttgagtttt tgccaggccg cgtcgatctc
     3421 gcgcgtcacg agcgcgcccg ccacgacatc cgaatggccg ttgagatatt tggtcgccga
     3481 gtgcatgacg agatcggcgc caagctcgag aggccgggtg aacaccggcg tcgccacggt
     3541 cgaatcgacc gcaacgcgcg cgccggcacg atgcgcaagc gcaacggccg cttcgatgtc
     3601 cgtgatgtcc cagaggggat tcgccggcgt ttcgacccag accattttcg tcttgccggg
     3661 aatgagcgcg cgggcgagcg cgccgctgtc gctcatgtcg atgaattctg cgccgaggtg
     3721 cgattggttg gcgagccagt ggcgcagcgc ccagtacatc accttgggcg ctacgacatg
     3781 gtcgcccggc gaaagggttt gaaaggcgca agttgccgcc gccatcccgg atgcaaacag
     3841 catcgcagcg gcgccgttct ccaactttgc cagcacggct tccggctgat cgaacgacgg
     3901 attgtcggcc cgcgcgtagt ttcggccgct gcggtagaga ttgtcggcgt cgcgctcgaa
     3961 ggtcgaagag aggtgaaggg gcagcacgac ggcgcgcgtt tcccgatcga cccagccgag
     4021 agcctgggcg gcgatcgtgt cggggtgatt cgcaaggctt tgtttgtcgt tcatgggttt
     4081 ctcggtcgga agatatccag gatgtcctgc ggcgagcgta ttccgccgcc tggcatttga
     4141 acatcgcacg ccaaagcgcg actggcgtca ggcggcgagg ccggtgtcgc cgggcgtctt
     4201 cgcgccgacg gcctcgggac ttgcgggact gagttgcgcg cacatccagc gcgtcgcttc
     4261 gcgcaacacc tctgcgctgc gtctccagtg caaaagcagg tgcggcgcgt cgggtacgac
     4321 acgccacgac tttggcccgt caagattgtc gaacagctgg cggatgcaac tctggcgcac
     4381 gagttcatcg cgggcgccga ccatggtgag aacgggaacg ttgacccgct ttgcttcgct
     4441 cacggacgcg agagcaagcg aggccatgcc gcaaagcatt tccggcggga tgcggcgcat
     4501 caccaatgga tcgccgagca accggatcgc ggcggccggc gtcaattccc agccgctcaa
     4561 gcgttcgaag accagttcgg cgtcgccccc cattgcccgc agggatgcag caaaccaggc
     4621 gagcaaccgc tgtcgaaaag gcgacgccag cgcgccggga gcggcaagca cgagtccttt
     4681 cagccgcggc gccatgccgt cggcggcggc atgaatcgcg actgcgccac ccagactctc
     4741 gccgatcgca aagactggcg catttgcggg cacacgtccc tgtacgaacc cggccgcctc
     4801 gatcatgtcg gcagccatgc gtgcagggcc tgtccaggcg ccgcgggagg cggtcgcacc
     4861 gaaaccgcgc tggtcgaagg cgagagacgt gaaaccgcgc ctcgcgagcc tcggtccgat
     4921 ctcctcgaat gccgcggcat agtcgaacgc gccgtgaagc aggagaaccg cggcgcgtgg
     4981 ttgccgcgcg agccagagac ggtagggcac ctccgctccg tccgatgccg cgaacgtgcc
     5041 acaccgcagg cgcgccctgg gatcgactcc cacgttttcc caatgaaacg agtcgacacg
     5101 cctgcgccgc gattcggcgc gcggctcagg aggcctgaag cggatgccga atcgataggg
     5161 gcgcgaaacg agaggccaca aactcatcgt cgtgcaatgc cgcgtccgac tctcaacccc
     5221 agcacaagaa agaccagaca gagcaccagg aagatcacaa acaggagttt ggcgatcgcg
     5281 aaggagaggc cggcgatacc gccgaagcca aggaccgcgg ctacaagccc cagaatcagg
     5341 aaaagaacgg caaggcg
//
LOCUS       DASZMC010000282         5321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3137614,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000282 DASZMC010000000
VERSION     DASZMC010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3137614"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VG274_05800"
     CDS             <1..851
                     /locus_tag="VG274_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE06201.1"
                     /translation="RILVTGGGTGLGEVMAEAYAKLGAAVYICGRRARVVEATAKRIS
                     DETGSQVKGLACDIRVPEAVDEMIAQIWSDGGALTGLVNNAAGNFISPTKDLSARGFN
                     AISDIVFRGTFYVTLASGKRWIEGGTHASVISILATWIWNGGPYTVPSAMSKAGLNIM
                     TKSLAVEWAPHGIRLNAIAPGPFPTKGAWERLNPDVAARGDSSGINPMGRVGQMPELA
                     NLAIFLMADGCDYLTGQTVAIDGGLHLATGGNFASLAKLGDSEWEHIRTAIRSANEKD
                     KAQRGV"
     gene            complement(1176..1937)
                     /locus_tag="VG274_05805"
     CDS             complement(1176..1937)
                     /locus_tag="VG274_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWE06202.1"
                     /translation="MTSFARSPDGLRIAYESAGEGEPVLLVHGFASSRAQNWRATGWY
                     EALTSAGRRVIAMDCRGHGESDKPTDPALYDYMLMAGDLISVLNAEGVGSSDIMGYSM
                     GGHLGLIILMERPEVLRRLIVGGVGETYLHGAFRSRFAIADALVEPDASRVTDPVQKT
                     FRAFAGQPGKDRNALAACMRGERKAYSPAELSLSTRPSLIVCGENDTISGPPGPLAAA
                     LHDGRAVSIPGRDHMSAVGDKRTKEAALAFIAERN"
     gene            2135..3526
                     /locus_tag="VG274_05810"
     CDS             2135..3526
                     /locus_tag="VG274_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWE06203.1"
                     /translation="MSLASDSPLPPRRRKSGPFSGMVQFLSGSDGYLRNILDNLPAAL
                     YTTDPEGRITYYNEAAAELWGYRPALGTSTWCGSWKLYWADGRPMRHDECPMAVAIRE
                     RRPVRGSEAIAERPDGSRVPFIPYPTPLYDSTGIFIGAVNLLVDAGDRKRADESAKRL
                     SAIVESSDDAIISKDLDGAITSWNSGAERLFGYTAEEAVGKPVTILIPEDRIDEEPEI
                     IERIRHGERIDHYETVRRHKHGSLIDVSLSVSPVRNADGRIVGASKIARDITERRRTQ
                     QQQSLLLNEMKHRVKNTLATVQALAMQTLHGASGAELAAFGARLSALGSAHDLLTLES
                     WNRLSLGDVVAAALKPFDEQKQERVVASGFGDAWLDGTKSLLLAMVLHELATNAVKYG
                     ALSNESGRVQLDWELDDRSRSVRLCWRESGGPPVTPPERKGFGSKLIEKSLGHGRFEY
                     RPEGLICTWEIAL"
     gene            3632..4000
                     /locus_tag="VG274_05815"
     CDS             3632..4000
                     /locus_tag="VG274_05815"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWE06204.1"
                     /translation="MTTASGHTTAILASKVKGTSVYNPKGDKIGHVEDVVLDKQTDRI
                     MFAALGFGGLLGMGEKYYPVPWSMLDYSEDRDGYVVPISKDVLDKAPSCSLDDLTKHD
                     GRFGEIRQRSYTYYKVPLDS"
     gene            complement(4202..4447)
                     /locus_tag="VG274_05820"
     CDS             complement(4202..4447)
                     /locus_tag="VG274_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06205.1"
                     /translation="MGGVGQSEGQLKQSIKTPPAPRDPEQRKALARGYRLRAEVSRLI
                     SEEVVLEETRTTLRGLADNYDFMASTLEKMNTSDGRE"
     gene            4638..>5321
                     /locus_tag="VG274_05825"
     CDS             4638..>5321
                     /locus_tag="VG274_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWE06206.1"
                     /translation="MPEDSIRGDMVNRLLASLSSTDFGLLEPHLEAVDLPVRKQLQTH
                     NRPIDHVYFIEHGIASVITRNSGKQGIEVGIIGREGLTGLAVIMETDRSPNDTFMQMA
                     GDGRRIGAGKLRASMEQSTTLRRALLRYGHSFIIQTAHTALANGRNKLLERLARWLLM
                     AHDRVDGDDIHLTHEFLSVMLGVRRTGITVALNQWARAGLVETRRGVITILDRRGLEE
                     ISNGSYGAPE"
BASE COUNT         1077 a         1652 c         1647 g          945 t
ORIGIN      
        1 agcgcattct cgtcaccggc ggcggaacgg ggctgggcga agtcatggct gaggcttacg
       61 cgaagctcgg cgcggccgtg tacatctgcg ggcggcgcgc gcgcgtggtc gaggcgaccg
      121 cgaaacggat ttcagatgaa acaggctcgc aggtgaaggg gctcgcctgc gacatccgcg
      181 tccccgaagc ggtggacgag atgatcgcgc aaatctggtc ggatggcggc gcgctgaccg
      241 gactcgtcaa caacgcggcg ggcaatttca tcagtccgac gaaagacctc agtgcgcgcg
      301 gcttcaatgc catctccgac atcgtcttcc gcggaacgtt ctatgtgacg ctcgccagcg
      361 gcaagcgctg gatcgaaggc ggcacgcacg ccagcgtcat ctcgatcctg gccacctgga
      421 tctggaacgg cggcccctac acggtgccgt ccgccatgtc gaaagccggg ctcaacatca
      481 tgacgaagtc gctggcggtc gaatgggcgc cgcacggaat caggctcaat gcgatcgcgc
      541 caggcccgtt tccaaccaag ggcgcctggg agcggctcaa tccggatgta gcagcccgcg
      601 gcgacagcag cggcatcaat ccgatgggcc gcgtcggcca gatgcccgag cttgccaatc
      661 tcgccatctt cctgatggcg gatggctgcg actatctcac cggccagacg gtcgccatcg
      721 acggcggtct gcatctcgcg acgggcggca atttcgcgtc gcttgcaaag ctcggcgatt
      781 ccgaatggga acacatccgc accgcaatcc gctcggccaa cgaaaaagac aaggcgcaac
      841 ggggcgtata gagaaaggca cgttcccgcc gaaccggttg ccccctccgc cccttcgggg
      901 cacctccccc gcgcgaaaag cgtgggggag gaaaatcatg atcgggtcag gtcgatcttt
      961 tcctcccccg cgcttggcgc gcgggggagg aaaagcgcga aaagcggggg gaggaaaagg
     1021 tcgaaatgcg cgggggagga aaaagccgag cgaacacgat cacgtctttc ctccctcgcg
     1081 catcgcgcgg gggaggtgcc cgcgaagcgg gcggaggggg cactttgcgc cgaacacata
     1141 tgattccagc cgatcgaacc gcacttcgga ggcgatcagt ttcgctccgc aatgaacgcg
     1201 agcgcggcct ccttcgttcg cttgtcgcct acagcgctca tgtggtcgcg gcccgggatc
     1261 gatacggcgc ggccatcgtg cagcgccgcg gcgaggggac cgggagggcc gctgatcgtg
     1321 tcgttctctc cgcacacgat caaactgggc cgcgtcgaca gtgacaactc cgccggcgaa
     1381 taagcttttc gctcgccgcg catgcaggcc gccagcgcgt tgcggtcctt gcccggctga
     1441 ccggcgaagg cgcggaaggt tttttgtacg ggatcggtaa cgcgggatgc atcgggctcg
     1501 acaagcgcgt cggcaatcgc gaaacgggag cgaaaagcac cgtgaagata ggtctcgcca
     1561 acacccccga cgatcaatcg gcgaagaacc tccggacgct ccatcagaat gatgaggccg
     1621 agatgtcccc ccatggagta acccatgata tccgaactgc ccacgccttc ggcgttgagc
     1681 accgaaatca gatcacccgc catcagcata taatcgtaga gcgccggatc ggtcggcttg
     1741 tcgctctcgc cgtgcccgcg gcaatccatg gcgatcaccc tccggcccgc actggtcagc
     1801 gcctcgtacc aaccggtcgc tctccagttc tgagcccggc tcgaggcaaa gccatgaacc
     1861 agcaacacgg gctcgccctc gccggcagat tcataggcga tgcgcaggcc gtcagggctg
     1921 cgcgcaaaag acgtcacgtg ccctttactc cccggaggtg gcctcccccg aattttctgt
     1981 cccggtgatc caatacgcaa cttctgcgtt acttgcgagg acaagctcgg gccatgaggg
     2041 cgtggatgac ggagttcgcg acagacgccg aagggggctg cgcgcgcagg agggcaacgg
     2101 ccaacgttcg cctcccggat cacatggtgg acacatgagt ctcgcgtccg attcccctct
     2161 tcccccccgg agacgcaaaa gcggaccgtt ctccgggatg gtccagttcc tgtccggcag
     2221 cgacggttat ctccgcaaca tcctcgataa ccttccggct gctctttata ccaccgaccc
     2281 cgagggccgg atcacctact acaatgaagc tgccgcagaa ctctggggat accgtcctgc
     2341 actgggcacg agtacatggt gcgggtcgtg gaagctgtac tgggcggacg gacgcccgat
     2401 gcgccacgac gaatgcccaa tggcggttgc gatccgggag cggcgaccgg ttcgcggctc
     2461 cgaagccatt gccgaacgac ccgatggcag ccgcgttccg ttcatccctt acccgacgcc
     2521 tctctacgat tccaccggca tcttcattgg cgcagtgaac ctgcttgtcg atgcgggaga
     2581 ccgcaaacgc gcggacgaat ccgccaaacg actctcggcc atcgtagaat cttccgacga
     2641 cgcgatcatc agcaaagacc tcgatggcgc gatcacgagc tggaactccg gcgcagaacg
     2701 actcttcggc tacacggccg aggaggcagt gggcaaaccg gttacaattc tgattcccga
     2761 agaccgcatc gacgaggagc ccgaaatcat cgagcgcatt cgccatggcg aacgtatcga
     2821 ccactacgaa acggttcgaa ggcacaagca tggtagtctg atcgacgtgt cgctctctgt
     2881 ttctccggta aggaatgccg atgggcgaat cgtgggcgca tcgaagattg cgcgcgacat
     2941 cacggagcgc aggcgcacgc aacagcagca gagcctgctt ctcaacgaga tgaagcaccg
     3001 cgtcaagaat acgctcgcga cggtgcaggc ccttgcgatg cagacgctcc atggcgcatc
     3061 gggggcagag cttgccgcgt tcggcgcgcg tctcagcgca ctcggaagtg cgcatgacct
     3121 gttgactctg gaaagctgga accggctgtc gcttggcgac gtggtagcag ctgcgctcaa
     3181 gccgttcgac gaacaaaaac aggagcgcgt cgtcgcttcg ggtttcggcg atgcgtggct
     3241 cgacggcacc aaatcgctcc tgttggcgat ggtgctgcac gaactcgcga cgaatgcggt
     3301 gaaatatggc gctttgtcga atgaaagcgg ccgggttcag cttgactggg aattggacga
     3361 ccgctcccgt agcgtgaggc tctgttggcg cgaaagcggc ggcccgcccg tcacgccgcc
     3421 agagcggaag ggattcggtt cgaagctgat cgaaaaatcg ctcggccacg ggcggttcga
     3481 ataccgtccg gaaggcctga tctgcacctg ggagatcgcg ctgtagcagc tgtccgtgaa
     3541 cgcgtatcca taaatcatcg cgatcggttg agacaaagga acgcacgcgt tgacacggcg
     3601 tttccctaca caacgtgatg ggagctcctc aatgacaaca gcaagtggac acaccactgc
     3661 catcctggcc tccaaggtca aaggcacctc ggtgtacaat ccgaaaggcg ataaaatcgg
     3721 ccacgtcgaa gacgtggttc tggacaagca gaccgatcga atcatgttcg cggcgctggg
     3781 attcggcggt ctgctcggca tgggcgaaaa gtactacccg gtgccgtggt cgatgctgga
     3841 ctacagcgaa gatcgcgacg gctacgtcgt tcccatatca aaagacgtgc tcgacaaagc
     3901 gccaagctgc agcctggacg acctgacaaa gcacgacggt cggtttggcg agatccgtca
     3961 gcgatcgtat acctattata aggtaccgct cgactcgtaa cctcggacca atcaacgcaa
     4021 aatgcggcga aggcgcctgc caacgtgggc gcctttgctg cgtccgccag ctacgcaggg
     4081 aatcctgatc aatacgcggt gttcttgcgt gacggcgcgg ctattggaac cgcgcaaata
     4141 ttatcacagc ctggcagatc ccatcgcccg tttcgcagac gctcgttcgg gagtttgtgt
     4201 gtcactcccg gccatctgac gtgttcatct tctccaatgt cgatgccata aagtcataat
     4261 tgtccgcgag gccccgcaat gttgtgcgcg tttcctccag caccacctcc tctgatatca
     4321 gccgtgatac ttcggcgcga agacggtacc cgcgcgccag cgctttgcgt tgttcaggat
     4381 cgcggggcgc ggggggagtc ttgatggatt gcttcagctg tccctctgac tgacctacgc
     4441 cgcccacgtc gtacctctca tggtgaaggg gacacgtcag ttctacagtc ttcccggcgg
     4501 gtatgcgtgg caagggggtc cgttctgcgt tttatgtacg aaaccgcaca gagcgggtat
     4561 ttgaaacgga caaagctctg cggcatcgtc tccctggaga ccgggcggcg gcgcccttcg
     4621 tgccagggga aaaggcgatg ccggaagatt ccatccgcgg cgacatggta aaccgcctgc
     4681 tcgcgagcct ctcgtcgacg gatttcggct tgctggaacc tcacctggag gccgtcgatc
     4741 ttcccgtccg caagcagttg cagacgcaca accggccgat cgaccatgtc tatttcatcg
     4801 agcacggcat cgcttccgtg atcacgagga actccggcaa gcagggaatc gaggttggga
     4861 tcatcggtcg ggaaggcttg acgggtttgg cggtcatcat ggagacggac cgttccccga
     4921 atgacacgtt catgcaaatg gccggcgatg gccggcgcat cggcgccggc aagctgcgcg
     4981 cgagcatgga acagagcacg accttgcgcc gtgcactgct gcgttacggg cattccttca
     5041 ttatccagac ggcgcatacc gccctggcca acggacgcaa caagctcctg gagcgcctgg
     5101 ctcgctggct gctgatggcg catgaccgcg tagatggaga cgacatccac ctcacgcacg
     5161 agtttctttc cgtcatgctt ggtgtgcgcc gcaccggtat caccgtcgcg ctcaaccagt
     5221 gggccagagc cggtcttgtc gaaacaaggc gcggcgtcat caccatcctc gaccgccggg
     5281 gattggagga aatttcgaat ggatcctatg gcgcgccgga g
//
LOCUS       DASZMC010000283         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3426525,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000283 DASZMC010000000
VERSION     DASZMC010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3426525"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VG274_05830"
     CDS             complement(<1..739)
                     /locus_tag="VG274_05830"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWE06207.1"
                     /translation="MLNPPLPFDELAQAIQRRRSTYGHISGLGLDRAAPGEAWSSLPY
                     RPAFVGDAESGVLHGGVVTAMLDESCGMAVQLALDGTRAIATLDLRIDYQKPATPGLA
                     IKAHSLCTRVTRSIAFVRSTAYQESEDDPVATATACFMIGANRANMLADSRIGDGVPP
                     MLDAPDDPSGPFANSPFARFLGIRVGEDGGLVMPFSPKIIGNPMLPAIHGGMTGAFLE
                     TAAIVGVTRDLGASAPPKPIGLTINYLR"
     gene            1074..>1700
                     /locus_tag="VG274_05835"
     CDS             1074..>1700
                     /locus_tag="VG274_05835"
                     /inference="COORDINATES: protein motif:HMM:NF044407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06208.1"
                     /translation="MNIALPVQSAASTVELVASAPSITNQPTTQDSDSQSAVTQIDGK
                     PEKSASTSPEPKRAYPAPASTPTQGSANGVRFDFNDGARIVCPQGEQPWQIRIRDLDT
                     GNVLFETEFAGGYVNSSKRYYVRFGIEVLQQGERVVDHQYSAKDRDVLIQFPVGTLGD
                     PMRWFPYAVKFKEKHGCKLSCAMGAPLIALFRDAYPDIEFVTHEEVKPE"
BASE COUNT          341 a          491 c          545 g          323 t
ORIGIN      
        1 agcgcaaata attgatggtg aggccgatcg gcttgggcgg cgcggaggcg ccgaggtcgc
       61 gggtcacgcc gacgatcgcc gccgtttcga ggaaggcgcc ggtcatgccg ccgtggatgg
      121 cgggcagcat cgggttgccg atgatttttg gcgagaacgg catcacgagc ccgccatcct
      181 cgccgacccg gatgccgaga aagcgcgcga acgggctgtt cgcgaacggg ccggacggat
      241 cgtccggcgc gtccagcatg ggcggcacgc cgtcaccgat ccggctgtcg gcgagcatgt
      301 tggcgcggtt cgcgccgatc atgaagcagg cggtcgcggt ggcgacggga tcgtcctcgg
      361 attcctggta ggcggtcgag cgcacaaagg cgatcgagcg ggtgacgcgg gtgcacagcg
      421 aatgcgcctt gatggcgagg ccgggcgtgg cgggcttctg gtaatcgatg cgcaggtcca
      481 acgttgcgat cgcgcgcgtg ccgtcgagcg cgagttgaac ggccatgccg cagctctcat
      541 ccagcatcgc ggtgacgacg ccgccgtgca ggacgccgct ctcggcgtcc ccgacgaagg
      601 cgggacggta gggcaggctt gaccaggctt cgccgggcgc ggcgcgatcg agcccgagtc
      661 cgctgatgtg tccataggtg gagcggcgcc gctggatcgc ctgtgccagt tcatcgaacg
      721 gcagcggcgg attgagcatc gatggttcgg acatgccgaa attgtaaacc accgttcgca
      781 ggggcaaaag gttaacggcg agggctaccc gccactcgct cgcaatagcg gctgacgaat
      841 attagcgatg atcaacataa accccccggt gttacccatt cggggtatgc gccggcgtgg
      901 cgggtcgctt attttcccct gcgtgcttct caaaagttaa ttttttgcag cgcaacgagg
      961 gctcaattat ccaaacttcg caaaacggct tcatgaaact ttcggagctg ttgactcggt
     1021 tgaaaagtta aaagaatgtt aatcgaaatc aatattttac tggttcttga aacatgaata
     1081 ttgcgctccc ggtgcagagt gccgcttcga ccgttgaact ggttgcaagc gcgccgtcga
     1141 tcaccaatca accgactacg caagattccg attctcaatc tgccgtgacg cagatcgatg
     1201 gcaaaccaga aaagtccgcg tcgacatcgc ccgagccgaa gcgcgcctat ccggcgcccg
     1261 catcaacgcc cacgcagggc agtgccaatg gggtgcgctt cgatttcaac gacggcgccc
     1321 gcatcgtttg tcctcagggc gaacaaccct ggcagatcag aatccgtgat ctcgataccg
     1381 gcaacgtact tttcgaaacc gagtttgccg gcggttatgt caacagcagc aagcgttact
     1441 acgttcgctt cggcatcgag gtgttgcagc agggcgagcg cgtcgtcgat caccaatatt
     1501 cggcaaagga ccgcgacgtc ctgattcaat ttcccgtcgg cacgctcggc gatccgatga
     1561 ggtggtttcc ttacgcggtg aagttcaagg agaaacacgg ctgcaagctc agctgtgcca
     1621 tgggcgcgcc gttgatcgcg ctgttccgtg acgcctatcc ggacatcgaa ttcgtaaccc
     1681 atgaagaggt gaagccggag
//
LOCUS       DASZMC010000284         7513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_409590,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000284 DASZMC010000000
VERSION     DASZMC010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409590"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..683)
                     /locus_tag="VG274_05840"
     CDS             complement(102..683)
                     /locus_tag="VG274_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWE06209.1"
                     /translation="MNAPARRYHHGDLHNALRDAARSILEKEGLSALSLRAVARQAGV
                     SHAAPYRHFASHEALLVELAREGFDELRHELVAAVSRPGLEDDRIASIGAAYMRFVAR
                     RPALVRLMFGPQLPNRDAFPELAAAADAVGAEIGTALADPALGLAVWAAVHGLAMLIL
                     ENVVDLGQRRSGLHVLPSRAEILLRSLFAGAQR"
     gene            complement(680..1567)
                     /locus_tag="VG274_05845"
     CDS             complement(680..1567)
                     /locus_tag="VG274_05845"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE06210.1"
                     /translation="MAQRVAFIGLGAMGYPMAGHLARAGHAVTVFNRTAARAQRWASE
                     HGGQTAPSPAAAAENADIVLACVGRDSDIDAVARGENGAFAAMKSGAVFVDHTTASAA
                     IARELAAAARALDLGFVDAPVSGGEQGARNGQLTIMCGGSEADFARIEPVIRLYARHS
                     QRMGEVGAGQLTKMVNQICIAGIVQGLSEGLSFARAAGLDPQVVVEVIAKGAAQSWQM
                     ENRHKSMLAGHFEHGFAVEWMRKDLAICTDEARRNGASLAMAALIDQFYGEVERMGGR
                     RWDTSSLFVRTEALRQGRQ"
     gene            complement(1787..3337)
                     /locus_tag="VG274_05850"
     CDS             complement(1787..3337)
                     /locus_tag="VG274_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWE06211.1"
                     /translation="MRAAGLTHEAYTTKLELRIVMLCSMAGTLMQALDSTIANVALPY
                     MQGSLAASRDQITWVLTSYIVAAAIMTAPVGWIAARFGRKNFAIVSLTGFTIASMMCG
                     AAQTLDQMIIFRLIQGAFGAALAPLSQSIMLDLYPPAKRGVVMAIWGMGVMVGPILGP
                     TLGGYLTDAYDWRWVFYVNLPFGIAAVTGISLFLRDTHRDESLKFDWFGFAVLGLGLG
                     ALQLMLDRGTTKDWFSSSEIIVYTVLACVGLYLFVVHILTTQKSFMPREMFKDRNFVT
                     ALFLMLIVSLIMLASSALLPPYLENLGGYSVTQTGLLMAPRGVGTMISMVFAGRLAMR
                     LDARHVMAAGTAFLLWSLWEMSHWTPDVAPWWLCFVSFVQGIGMGLIFVPMNLIAFAT
                     LSPVLRTDGSAMFNLIRNVGSAIGVSITTTMLANSIQTVHAQLAGNVNPFNRALSVNA
                     PSMMWNPHLPFGVAQLDSIVQYNAQIIAYANDFLFMFFISLPALFVLFLMRRPALLQR
                     PPEIEVME"
     gene            complement(3504..4643)
                     /locus_tag="VG274_05855"
     CDS             complement(3504..4643)
                     /locus_tag="VG274_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWE06212.1"
                     /translation="MAAIQTDALVSPYVAAGRAPSAPGLIARLKADRTLVRRVLMIGG
                     VAIVAAASLAFWLMGDRYIGTDDSYVHAAQLMVSTDVSGLVTDVNVHQGQHVRKGQVL
                     FRIDPRQFRIALDNAKANLGQTQLTLLSMKDDYARMLNDIAAQQSQVALDQVTFSRYV
                     ALLRVNSIAQAQYDQAHFTLATAQSTLASLKEQAQTQLAKLGGNIATPVERLPQYLAA
                     QSQVDEAQRQLDHTVVRAPFDGIVTEVDSLQPGTLVVSALSAFTTTSAVGLVSTSNTW
                     VEANMKETDLTHVHRGDPVDVTVDTYPGRTWTGEVDSISPATGSDFSVLPAENASGNW
                     VKVTQRVTVKVRLDLRPGDPPLRAGMSTYVTIDTGHRRWFRMLYG"
     gene            complement(4648..5100)
                     /locus_tag="VG274_05860"
     CDS             complement(4648..5100)
                     /locus_tag="VG274_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWE06213.1"
                     /translation="MQQFDRDLMLLLHDVARLLRTRFDQRARARGMTRAQWVILLRLN
                     AQPGLSQIEMASLLEVEPISVGRLIDRLEARGLVERRPDPSDRRVWRLHLLPSAQPIL
                     DEISRTREVLGAELFDGVDATARATMIDALLRMKNNLTAEPAPIAVGE"
     gene            complement(5233..5724)
                     /locus_tag="VG274_05865"
     CDS             complement(5233..5724)
                     /locus_tag="VG274_05865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWE06214.1"
                     /translation="MIFDTRAFRTALGCFPTGVAVVTAESRDLHPKGITVNSFTSVSL
                     DPPLVLWCVDRGSDRCETFITACGFTVSILDAAHRGLSARLARRGEHRLDGITLLPTE
                     LGAPALAEALAVFECARESVHQGGDHMILLGRVLRFSFRKAGAPLVFYRGRYVGLADR
                     EAD"
     gene            complement(5748..6872)
                     /locus_tag="VG274_05870"
     CDS             complement(5748..6872)
                     /locus_tag="VG274_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWE06215.1"
                     /translation="MSPGAFLIAEREFRTYVATLSFWLALAAGPLLTAAVFALAPAAG
                     SSATVAVAIHAEDPALARSALAALSDVGRIESRHFRFVSKNERASLALRNSDNAVTMR
                     FSSRFPLSTEGRVLVARTLERDLAQGRASAGRSRALSVQVREISDTALPTREADAAGS
                     RFVLVAILWLTLTGSLGMLLQAVVRERANRALEGLLSAVRPWEIMTGKLVGVGAVSAL
                     VFVVWLGCVAALPALAPHGEDAASNVLAHLASPESLLRTAAIYLLAWLFYGTVTFGLG
                     AAARDVAAAQNMSRPMFAVLLAAFFVALACAFGARDSWLLYIPPFTPFLLLMHAPTEL
                     SLVSQLEAVGLVLLATAFAGWLAIGRLTVQNVWAARNAQA"
     gene            complement(7061..>7513)
                     /locus_tag="VG274_05875"
     CDS             complement(7061..>7513)
                     /locus_tag="VG274_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWE06216.1"
                     /translation="LIRRDATVFTLRPFDWAIALGGTALALFVRPGGHPFLPSFVGTA
                     FMSAGLLLAIISKLTLRRSFGVAAANRGIVRAGPYNLVRHPMYAGYILVYAGFLLNNP
                     LWWNLGVYFATMLCLIARILAEEKVLAKDADYADFMTRVRYRLAPGLF"
BASE COUNT         1486 a         2383 c         2440 g         1204 t
ORIGIN      
        1 ggttcgacag gctcaccatg agggttcgac aggctcgccg aggacctcgt ccagagcctg
       61 gcgaggacct catcctgagc ctgtcgaagg atggcgaccg ctcagcgttg cgcgccagca
      121 aacaggctgc gcaggagaat ctcggcgcgt gaaggcagca cgtgaagtcc ggagcgccgc
      181 tgtccgagat cgacgacgtt ttcgagaatc agcatggcga ggccgtggac cgccgcccac
      241 accgcgagcc ccaaagccgg gtcggcgaga gcggtgccga tctccgctcc gacggcgtcg
      301 gcggcggcgg ccaattcggg aaacgcatcg cggttgggca attggggtcc gaacatcagc
      361 cgcaccagcg cgggccgccg cgcgacgaag cgcatatagg cagcgccaat gctcgcgatg
      421 cggtcgtctt caagacctgg ccgggagacg gcggcgacaa gctcgtggcg gagctcgtcg
      481 aagccttccc tggccaattc gacaagaagc gcctcatggc tcgcgaagtg ccggtacggg
      541 gccgcgtggc tgacgcccgc ctgccgcgct actgcgcgca ggctgagggc ggaaagcccc
      601 tccttttcaa gaatcgagcg tgcggcatcg cgcaacgcat tgtgcaggtc gccgtggtga
      661 tagcggcgcg ccggcgcgtt cactgacgcc cctgccggag cgcttcggta cgcacgaaga
      721 ggctggacgt gtcccagcgc cttcccccca ttctctccac ctccccgtaa aactggtcga
      781 tcagagctgc catcgcgagc gaagcgccgt tgcgccgcgc ttcgtcggtg cagatggcca
      841 gatccttgcg catccactcc accgcaaatc cgtgctcgaa atggccggcc agcatgctct
      901 tgtgccggtt ctccatctgc caggactgcg ccgcgccttt tgcgatcacc tcgacgacaa
      961 cttgcggatc gaggcccgcc gcacgggcga agctcaatcc ttccgacagg ccctgcacga
     1021 ttcccgcgat gcagatctgg ttgaccatct tggtcagctg ccccgcgcca acctcgccca
     1081 ttcgctggct gtgccgcgca tacaggcgga tcaccggttc gatcctcgcg aaatcggctt
     1141 cggatccacc gcacatgatg gtgagttgac cgttgcgggc gccctgctcg ccgcccgaga
     1201 cgggcgcgtc gacgaagccg agatcaaggg cgcgcgccgc ggccgcaagt tcccgggcaa
     1261 tcgcagcgga cgcggtcgtg tgatcgacaa ataccgcgcc cgatttcatc gcggcaaacg
     1321 cgccgttttc gccgcgggcg acggcgtcga tatcgctgtc gcgcccgacg caggcgagca
     1381 cgatgtcggc attctcggca gccgctgccg gtgacggggc ggtttgcccg ccatgctcgc
     1441 tcgcccagcg ctgcgccctc gcagccgtgc ggttgaacac ggtcaccgcg tgacccgcgc
     1501 gggcgagatg gcccgccatg ggatagccca tcgcacccag gccaatgaaa gcgacgcgct
     1561 gcgccatcgt gaatctccgc ctttcaacca gggagacagt taaccgccaa gacgccaaga
     1621 cgccaagacg cccgtagcgc gacgaagatt cacaatgggg cagtgagaat tgttgcgcgc
     1681 ctgcggcgcg ccgcctctct cctcaccgtc tcactgcctc attgtgattc ttcttcttgg
     1741 cggcttggcg gttacgttcg cggcagaaaa atcggatccc gccgcgctac tccatcactt
     1801 cgatctcggg cgggcgttgc aggagcgcgg ggcggcgcat caggaacagc acgaataacg
     1861 cgggaagcga gatgaagaac atgaacagga agtcgttcgc ataggcgatg atctgcgcgt
     1921 tgtactgcac gatcgaatcg agctgggcca ctccaaacgg cagatgcgga ttccacatca
     1981 tgctgggcgc gttcacggac agagcgcggt tgaagggatt gacgttgccg gcgagctgcg
     2041 cgtgcacggt ctgaatgctg ttggcaagca tggtcgtggt gatcgaaacg ccgatggcgc
     2101 tgccgacatt gcggatgaga ttgaacatcg ccgagccgtc ggtgcgcagt acgggcgaca
     2161 gcgtcgcgaa ggcgatcagg ttcatcggca cgaagatcag gcccattccg atgccctgaa
     2221 cgaacgacac gaagcagagc caccatggcg cgacatccgg cgtccaatgg gacatttccc
     2281 atagactcca caggaggaag gcggtgcccg ccgccatcac gtgccgcgcg tcgagccgca
     2341 tcgcgaggcg gcctgcgaaa accatcgaga tcatggtgcc gacgccgcgc ggcgccatca
     2401 gcagtccggt ctgggtgacc gaatacccgc cgagattctc gagatagggc ggaagcagcg
     2461 ccgaactcgc gagcatgatg aggctcacga tcagcatcag gaacagcgcg gtgacgaaat
     2521 tgcggtcctt gaacatctcg cgcggcatga aggatttctg cgtcgtcagg atgtgcacga
     2581 cgaagagata gaggccgacg caggcgagca cggtgtagac gatgatctcg ctcgaggaga
     2641 accagtcctt tgtcgtgccc cggtccagca tcagctgcag cgcgccgagg ccgaggccca
     2701 gcacggcaaa gccgaaccag tcgaatttga gactctcgtc gcggtgcgtg tcgcgcagga
     2761 acagcgagat gcccgtcacg gcggcgatgc cgaacggcag attgacgtag aacacccagc
     2821 gccaatcgta ggcatcggtc agatatccgc cgagtgtcgg tccgaggatc ggccccacca
     2881 tcacgcccat gccccagatc gccatcacca cgccgcgctt tgccggcgga tagaggtcga
     2941 gcatgatcga ctgggagagg ggcgccagag ctgcaccgaa cgcgccctgg atcagacgga
     3001 agatgatcat ctgatcgaga gtctgcgccg cgccgcacat catcgaggcg atggtgaacc
     3061 ccgtcagact cacgatggcg aaattcttgc gcccgaaccg cgccgcgatc cagccgacgg
     3121 gagcggtcat gatcgccgca gcaacgatgt aggaggtgag gacccaggtg atctggtcgc
     3181 gcgaggcggc aaggctgccc tgcatgtaag gcagagcgac attggcgatc gtgctgtcga
     3241 gcgcctgcat gagcgtgccc gccatcgagc acagcatcac gatgcgaagc tcgagcttcg
     3301 tcgtgtaggc ttcgtgcgta agtcccgcag cgcgcatcaa acaaagcggt gccggttgcg
     3361 tggccgaaga agactcttaa cgcggaggac gcagaggttt cgcggaggac gcgggagaag
     3421 aaggatggcg cgccgaaggc gcgcaatttt cgctgcgcac ctctgcgcat cctctgcgtc
     3481 ctctgcgtta aaaatctttc gcctcatccg tagagcatgc ggaaccagcg gcggtggccg
     3541 gtgtcgatgg tgacgtaggt gctcatgccg gcgcgcagcg gcggatcgcc gggcctcaga
     3601 tcgagtctga ccttcaccgt aacgcgctgg gtaaccttga cccagttgcc gctcgcgttt
     3661 tcggccggca gcacggaaaa atccgatccc gttgcggggc tgatggaatc cacctcgccc
     3721 gtccacgttc ttcccggata ggtatcgacg gtgacgtcga ccggatcgcc gcggtgtaca
     3781 tgggtcagat cggtctcctt catgttcgct tcgacccatg tgttgctggt ggagacgagc
     3841 ccgactgcgc tcgtcgtcgt gaaggcggag agcgcagaaa cgaccagcgt tccgggctgg
     3901 agcgaatcca cttcggtcac gatgccgtcg aacggcgcac gcacgacggt atgatcgagc
     3961 tgccgctgcg cctcatcgac ctgcgactgc gcggcgagat attgcggaag ccgttcgacc
     4021 ggcgtggcga tgttgccgcc gagcttggcg agctgtgtct gcgcctgctc cttcaacgaa
     4081 gccagcgtgc tctgtgccgt ggcgagcgtg aaatgtgcct ggtcgtattg cgcctgcgcg
     4141 atactgttga cgcgcagcag cgccacgtag cggctgaagg taacctgatc gagcgcgacc
     4201 tgggactgtt gcgccgcgat atcgttaagc atgcgtgcgt aatcgtcctt catggagagc
     4261 agcgtcagct gcgtctggcc gagattggct ttcgcgttgt cgagcgcgat gcggaattgt
     4321 ctggggtcga tgcggaacag tacctgccct ttcctgacgt gctggccctg gtgcacgttc
     4381 acgtcggtga cgaggccgga gacatcggtc gacaccatca gctgcgcggc gtgcacgtag
     4441 gaatcgtcgg ttccgatata gcggtcgccc atcagccaga aggccagcga ggccgcagcc
     4501 actatggcga cgccgccgat catcagcacg cggcggacca gcgtgcggtc ggccttcagg
     4561 cgcgcaatca ggcccggcgc agagggggca cgacctgccg ccacataggg cgagacgaga
     4621 gcgtcagttt gaatcgcagc cacgttccta ttctcccact gcaatcggcg caggctccgc
     4681 cgtcagattg ttcttcatgc gcaacagcgc atcgatcatg gtcgcgcgcg cggttgcatc
     4741 gacgccgtcg aaaagttcgg cgccgagcac ttcgcgcgtc ctggaaatct cgtcgaggat
     4801 cggttgcgcg gatggcagga ggtgcagccg ccagacccgg cggtctgaag gatcgggccg
     4861 gcgctcgacc agtccgcggg cttccagcct gtcgatcaac cggccgacgc tgatcggctc
     4921 gacctccagc agcgaggcca tctcgatctg cgacagaccc ggctgggcat tcaggcgcag
     4981 caaaatcacc cattgggcgc gggtcatgcc gcgggcgcgg gcgcgctggt cgaaccgcgt
     5041 gcgcagaagc cgcgccacgt cgtgaaggag aagcatgagg tcgcgatcga attgttgcac
     5101 tgcagtatcc tctgagcagc gagaatataa gcagcgctat tataacgcac aagatgtatt
     5161 atcggttaat cgcctggccg gtagcacagt tctgagaatt gaatcccaaa aattgtcatg
     5221 gaatatcgca attcaatccg cttcccggtc cgcaagcccc acgtagcggc cccggtaaaa
     5281 gaccaggggc gcgccagcct tgcggaagga aaaccgcagc acgcgtccca gcagaatcat
     5341 atggtcgccg ccctgatgga cggattcgcg cgcgcactcg aagaccgcga gggcctcggc
     5401 cagcgccgga gcgccaagct ccgttggcag gagcgtaatc ccgtcgaggc ggtgctcccc
     5461 tcgcctcgcg agcctggccg aaaggccgcg atgtgcggca tcgaggatgc tgaccgtgaa
     5521 tccgcacgca gtaatgaaag tctcgcagcg gtcggaaccc cggtcgacgc accacaacac
     5581 cagaggggga tcgagggaga ccgaggtgaa ggaattgacg gtgatccctt tgggatgcag
     5641 atcgcgactc tcggcggtca ccaccgcgac gccggtggga aagcagccca aggcagtgcg
     5701 gaacgcgcgc gtgtcgaaga tcatgaaaac cctgcccggg cgacgaatca ggcctgcgcg
     5761 ttgcgggccg cccagacatt ctggaccgtc aggcggccaa tcgccagcca gccggcgaaa
     5821 gccgtggcca gaagcaccaa accaaccgcc tccagctgcg acactagaga caactcggtg
     5881 ggcgcgtgca tgagaagcag gaagggcgtg aacggcggaa tgtagagcag ccagctgtcc
     5941 cgggcgccga atgcgcacgc aagggcgacg aagaacgccg ccagcagaac cgcgaacatg
     6001 gggcgtgaca tattctgcgc ggcggccacg tcgcgcgccg ccgctcccag cccgaatgtg
     6061 accgttccgt agaagagcca ggcgagcaga taaatcgccg cggtgcgcag cagcgattcg
     6121 ggcgatgcga ggtgggcgag cacgttcgac gcggcgtcct ctccgtgcgg cgccagcgcc
     6181 ggcaaagccg ccacgcaacc cagccacacc acaaagacca gcgccgaaac ggcgccgact
     6241 cccaccagtt ttccggtcat gatctcccag ggccgcaccg ccgacaacaa tccttccagc
     6301 gcccgattgg cgcgttcgcg cacaacggcc tgcaagagca tgccaagaga tcccgtcagc
     6361 gtcagccaca ggatggccac caacacgaag cgtgaccccg cggcgtcagc ctcgcgggtc
     6421 ggcaatgcgg tgtcggagat ttcgcgcacc tgcacggaga gcgcgcggct gcggcccgcg
     6481 gatgcccggc cttgcgccag atcgcgctcc agcgtgcggg cgaccagcac acgtccctcg
     6541 gtactgagtg ggaaccggct gctgaagcgc atggtgactg cgttgtcgct gttccggagc
     6601 gccagcgagg cacgctcgtt cttcgagacg aaacggaaat gcctgctctc gatccggccg
     6661 acgtcggaga gcgcggcgag ggcggatcgt gcaagtgccg gatcttcagc atgaattgcg
     6721 acggccaccg tcgcagagct gccggccgcc ggcgcaagcg cgaaaacggc cgccgtcaga
     6781 agcggacctg cggccagcgc gagccagaaa ctcagcgtgg cgacgtaagt gcggaattcg
     6841 cgttcggcga tcagaaatgc acccgggctc atctagtggc ccagttctaa caattgaatc
     6901 ccaaaaaaat tgtcatggcc ccgtttggcg gggccaccca gcaagcgcgc gtccgcgcgc
     6961 tgaatatgcg ctctgagcgg tttcacctgg gtggcccgct ctgcggcggg ccatgacaaa
     7021 ttttgggtgt gaagtgttgg aatcggaccg ctagcctcca tcaaaaaagg cccggcgcga
     7081 gccggtagcg cacgcgggtc atgaagtccg cgtagtcggc atctttcgcc agaacctttt
     7141 cttcggcgag gatgcgcgca atcaggcata acatggtcgc gaaatagact ccgagattcc
     7201 accacagcgg attgttcaac aggaaccccg catagacgag aatgtagccg gcatacatgg
     7261 gatgcctgac aagattgtac ggtcccgccc ggacgatccc gcggtttgcg gccgcgacgc
     7321 cgaaactgcg ccgcaacgtc aatttggaga tgatcgcaag cagcaggccg gccgacatga
     7381 aagctgtccc cacaaaggag gggaggaagg gatgcccgcc cggccttacg aaaagcgcca
     7441 acgccgttcc gccaagcgcg atcgcccagt cgaacggacg cagtgtaaac accgtcgcgt
     7501 cgcgccgtat gag
//
LOCUS       DASZMC010000285         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2320289,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000285 DASZMC010000000
VERSION     DASZMC010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320289"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VG274_05880"
     CDS             <1..897
                     /locus_tag="VG274_05880"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HWE06217.1"
                     /translation="RTDGLFRALVDCEGQPLDEPATLYDQAFALLAMASLHKLPGAPP
                     GFDADAGKLLAAIEGSMRHEAGGFREQANSPFQSNPHMHLLEAALGWSEAGGGTAWDA
                     LADEIVALCLKRFIDPDEGFVREHFTAGWQPALGDLGRLVEPGHQFEWATLLARWARL
                     RREPAAELAARRLFEAGVRGIDTVRGVAIDALNEDMSARSSRARLWPQTERLKAALLF
                     AGAADGAANTYAEHAVAAAQSLWKYLDVPTRGLWRDKLTPTNRFVEEPAPASSLYHIV
                     GAVAALKHSLAAREPAISLAAQ"
     gene            972..2258
                     /locus_tag="VG274_05885"
     CDS             972..2258
                     /locus_tag="VG274_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWE06218.1"
                     /translation="MKDDSGIPIPARFAEALVDPSAYADGRIHETWRWLRANNPLGIA
                     QAEGFDPFWVATKHADILAVGRQNDLFHNGDRPTTLTGQAGDRRVREITGGSPHLVYS
                     LVQMDPPDHPKYRALTQAWFMPPNLKKLEERIRAIAKRTVDAMCAKPGRCDFVTDVAA
                     GYPLHVIMEIMGVPEEDEPRMLRLTQELFGPQDPDTARALSALSPEQLTALIQATIED
                     FSSYFRRLSAERRARPREDLATLIANAKIDGEPISDIGAMGYYIIVATAGHDTTSSST
                     AGAVWALAENPGEFAKVKGEPSLIPGLVDEAIRWTTPVKHFMRSATADTQLAGRAIAK
                     GDWLMLCYASGNRDEEVFEDPDVFRVDRKPNRHLSFGYGAHLCLGQHLAKMEMRILFE
                     ELLPRLKSLALDGEPKLTQALFVNGPKTVPIKFALA"
     gene            complement(2467..2874)
                     /locus_tag="VG274_05890"
     CDS             complement(2467..2874)
                     /locus_tag="VG274_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE06219.1"
                     /translation="MNQVQFRTFSPPGGIPTTVGGCPLRTAVGAIGGRWKPLVLHYLL
                     QQPRGFGELSRVLECVANKTLATQLRELERDGLISRTVNSDARRSVCYAPTPDGIAMQ
                     PVIRALVEWGNRRLEAARAARTALAETNSPGGG"
     gene            2927..>3410
                     /locus_tag="VG274_05895"
     CDS             2927..>3410
                     /locus_tag="VG274_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06220.1"
                     /translation="MVSRRAIAFITAGLLAASPLIAEEAPPPAVPAPQLRDGSHDFDF
                     ERGAWKLHISRLVHPLAGSTEWTEYDGAKTDIPIWSGKANLAEVNAEGPKGHLQFLAL
                     RLYNPAAHQWGLYFASANSGVFGTPLVGEFRNGRAEFIGPDTYNGRSILVRFVMQSDG
                     P"
BASE COUNT          612 a         1138 c         1074 g          586 t
ORIGIN      
        1 cgaacggatg gcctgttccg cgccctcgtc gattgcgaag gccaaccgct cgacgaaccg
       61 gccacgctct acgaccaggc tttcgccctg ctggcgatgg cgtcgcttca caaattgccc
      121 ggcgcgccgc caggattcga cgcggacgcc gggaagctgc tcgccgcgat cgaaggctca
      181 atgcgtcacg aagcgggcgg cttccgcgag caggcgaaca gcccatttca gtccaatccc
      241 cacatgcatc ttctggaagc ggcgctcggc tggtcggagg ccggcggcgg cacggcgtgg
      301 gacgcgctcg ccgacgaaat cgtggcgctc tgcctcaaac gtttcatcga tcccgacgaa
      361 gggtttgtgc gcgagcattt caccgcaggc tggcagcccg ctttgggaga tctcggccgc
      421 ctcgtcgaac cgggacatca gtttgaatgg gccacgctgc ttgcgcgctg ggcgcggttg
      481 cgcagggagc ccgcggccga gcttgcggcg cggcgtctgt tcgaggcagg tgtgcgcggc
      541 atcgatacgg tgcgcggggt cgccatcgat gcgctcaacg aagacatgtc ggcgcgatcg
      601 agccgcgcgc ggctctggcc gcagacggag cggctcaagg cggcgctgct gttcgccggc
      661 gcggcggatg gggccgccaa tacctatgcc gagcacgcgg tcgccgccgc gcaatcgctg
      721 tggaaatatc tcgacgtgcc gacgcgcggg ctctggcgcg acaagctgac gccgaccaac
      781 cgcttcgtcg aggagcccgc gccggcaagc tcgctctatc acattgtcgg cgccgtcgcg
      841 gcgcttaaac attcgctcgc cgcgcgcgag cccgccattt ctctcgccgc gcagtgatgg
      901 ttcgacaggc tcaccatgag gacctcatcc tgagcttgtc gaaggatgat tgatggaggg
      961 tatgggcggc gatgaaggat gacagcggca tcccgattcc cgcgcgcttc gccgaagctc
     1021 tggtcgatcc ctccgcctat gccgacggac gcatacacga aacctggcgc tggctgcgcg
     1081 ccaacaatcc gctcgggatt gcccaagcgg aaggattcga tcccttctgg gtggcgacga
     1141 agcacgccga tattctcgcc gtcggccggc agaacgatct cttccacaat ggcgaccgtc
     1201 cgacgacgct gaccggccag gcgggcgacc gccgcgtgcg cgaaatcacc ggcggcagcc
     1261 cgcatctcgt ctattcgctt gtacagatgg atccgcccga ccatccgaaa taccgcgccc
     1321 tgacgcaggc gtggttcatg ccgcccaatc tcaaaaagct cgaagagcgc atccgcgcga
     1381 tcgcaaaacg caccgtcgat gcgatgtgcg caaaaccggg ccgttgcgat ttcgtgacgg
     1441 atgtcgcggc gggttacccg ctgcatgtga tcatggagat catgggcgta ccggaagaag
     1501 acgaaccgcg catgctccgc ctcacccagg agttgtttgg tccgcaggac cccgatacgg
     1561 ccagggcctt gagcgcgctg agcccggagc agctgacagc gctgatccag gcgaccatcg
     1621 aggatttcag ttcctatttc cgccggctga gtgcggagcg ccgcgcccgt ccgcgcgaag
     1681 acctcgcaac gctgatcgcc aatgcaaaga tcgacggcga gccgatctcc gatatcggcg
     1741 cgatgggcta ttacatcatt gtcgccaccg cgggacatga tacgacgtcg tcatcgacgg
     1801 ccggcgccgt gtgggcgctc gccgaaaatc ccggcgagtt cgcgaaagtg aaaggcgaac
     1861 cgtctctgat tccggggctt gtcgacgaag cgatccgctg gacgactccg gtcaagcact
     1921 tcatgcgttc cgccacggcg gatacgcaac tcgcaggccg cgcgatcgcg aagggtgact
     1981 ggctgatgct ctgctacgcg tcgggcaatc gcgacgagga ggtgttcgag gatcccgatg
     2041 tcttccgcgt cgaccgcaag cccaaccggc acctgtcgtt cggctacggc gcgcatctct
     2101 gtctcggcca gcacctcgcc aaaatggaaa tgcgcattct attcgaagag ctgctgccgc
     2161 gcttgaaatc cctcgcactc gacggcgagc cgaagctcac tcaggccctc ttcgtcaacg
     2221 gccccaagac cgtgccgata aagttcgcgc tggcgtgaag gggcccctca cccgcgccga
     2281 agcagaattc gacgcacgta tctgcatccc tctcccacga ggggagaggg gaatctgaat
     2341 ctcgcatcaa gggcggaaaa tcagcaggta acgttccctc tccccttgtg ggagagggcc
     2401 taagtacgac acctctctac cacttcagcg cgggtgaggg ggaacacggg cgcttatttc
     2461 gaagcttcac ccacccccgg gggaattcgt ttccgccagc gccgtcctcg ccgcacgtgc
     2521 cgcttccagc cgcctattgc cccattccac cagcgcgcgg atgacgggct gcatcgcgat
     2581 accgtctggc gtcggcgcgt aacagacgct gcgccgcgcg tcgctgttca ccgtgcggga
     2641 gatcagcccg tcgcgctcga gctcacgcaa ttgtgtggcc agcgtcttgt tcgccacgca
     2701 ttcgagcacg cgggaaagtt cgccgaaacc gcgcggctgc tgaagcaggt aatgcagcac
     2761 cagcggcttc cagcgcccgc cgatggcgcc gaccgcggtg cgcaacgggc agccgccgac
     2821 ggtggtggga atgccacctg gtggcgaaaa ggtacggaat tgaacctggt tcatcgcgcg
     2881 ccctaactct gtgtcggcgc cgcatcataa cagcgaggga gtttccatgg tatcgcgcag
     2941 agccattgcg ttcatcactg ctggcctgct tgccgcatcg ccgctcatcg cggaagaggc
     3001 gccaccgccc gccgttccgg ccccgcagct tcgcgacggc agccatgatt tcgatttcga
     3061 gcgtggcgca tggaagcttc acatcagccg cctcgtgcat ccgctcgcgg gttcgaccga
     3121 atggacagag tacgacggcg ccaagaccga catccccatc tggagcggca aggccaatct
     3181 cgcggaagtg aatgcggagg ggccaaaggg ccatctccaa tttctcgcgc tgcgcctcta
     3241 caaccccgcg gcgcatcaat ggggccttta tttcgcgagc gccaattcgg gcgtgttcgg
     3301 cacgcccctt gtcggcgaat tccgcaacgg ccgcgcggag ttcatcgggc ccgacacgta
     3361 taatggccgc tccattctcg tccgcttcgt gatgcagagc gacgggcccg
//
LOCUS       DASZMC010000286         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3674662,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000286 DASZMC010000000
VERSION     DASZMC010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3674662"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VG274_05900"
     CDS             complement(<1..1055)
                     /locus_tag="VG274_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE06221.1"
                     /translation="MSVAAESLPRQSASVSIGATVVPLLAIAIFINYVDRGNLATAAP
                     LMNNELELSATQLGLLLSMFFWSYTPCQILAGWLAEKITATRVLAIGLAIWSLATAAT
                     GLVSGFAALLVLRLLLGVGESAAFPCSSKLLARHLPPHRLGSANGAIAVGLALGPAFG
                     TFVGGMLMARVGWRPVFLLFGLASLTWLLPWLWATRDPSLGHDETRDAPAPGFLDILR
                     HREAWGACLGHFTGNYTFYFVISWLPLYLVHERGFTVAEMSVLGGMIYLVYAASASVA
                     GWGSDRWMQSGASANRVRKTVIVAGGVFAAAAMLGCAFGNAAVSVASLFLAAFSFGLG
                     TPTIYAIGQTLAGPRAAG"
     gene            complement(1081..1278)
                     /locus_tag="VG274_05905"
     CDS             complement(1081..1278)
                     /locus_tag="VG274_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06222.1"
                     /translation="MEIGALGHVLGRVTDSVAPHPVDHEVTKSLYFADSDGNGVEQYI
                     DASDVWRREPQRVAYAAPMGS"
     gene            1727..>2242
                     /locus_tag="VG274_05910"
     CDS             1727..>2242
                     /locus_tag="VG274_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWE06223.1"
                     /translation="MFRQKLGWTLAFSTAVIIAAMALDYVISIVILKNAAGYTPYITL
                     TISTLVAFPVTFAIVSAWINMRHARDELSVAHRAADQARAIALQALRDVDVARQLADS
                     ERTAALEASRAKSEFLANMSHELRTPLNAILGFSEMLKSGMRPGKTEEYSEFIHRSGR
                     HLLSLINDILDL"
BASE COUNT          440 a          753 c          675 g          374 t
ORIGIN      
        1 ccggcggcac gcgggcccgc gagcgtctgt ccgatggcat agatcgtcgg cgtgccgaga
       61 ccgaacgaaa acgccgccag aaaaaggctg gccacggata ctgccgcgtt gccgaaggcg
      121 cagcccagca tggcggccgc cgcgaaaacg ccgccggcga cgatcaccgt tttgcgcaca
      181 cggttcgcgc tggcgccgct ctgcatccag cggtccgacc cccaacccgc gactgacgcg
      241 ctcgcggcat agacgagata gatcatcccg ccgagcaccg acatctcggc cactgtgaaa
      301 ccccgttcat gcacgagata gagcggcagc cacgagatca cgaaatagaa cgtgtaattg
      361 ccggtaaaat gaccgaggca tgcgccccat gcctcgcgat ggcgcagaat atcgaggaaa
      421 cccggcgccg gcgcgtcccg cgtttcatca tggccgagag atggatcccg cgtcgcccac
      481 agccacggca agagccatgt cagcgaggcg aggccgaaca gcaggaagac cgggcgccat
      541 ccgactcgcg ccatcaacat tccgccgacg aaggtcccga acgcaggccc gagcgccagg
      601 cccacggcga tcgcaccatt ggcgctgccg agccgatgcg gaggcaggtg ccgggcgagc
      661 agtttcgagc tgcagggaaa cgccgcgctt tcgcccacgc cgagcagcag cctgagcacg
      721 aggagcgcgg cgaatccgga caccagcccc gtcgctgcgg tggcgagcga ccagatggcg
      781 aggccgatcg ccagaacgcg cgtcgcggtg atcttctctg ccagccagcc ggccaggatc
      841 tggcagggcg tatagctcca gaagaacatc gagagcagca gcccgagctg cgtggcggag
      901 agctccagct cgttgttcat caacggcgcc gccgtcgcca gattgccgcg gtcgacgtag
      961 ttgatgaaga tggcgatcgc cagcagcggc acgacggttg ccccgatgct tacgcttgcg
     1021 ctttggcgcg gcaagctctc cgcagcgaca ctcatgcgcg ttcgctccga gcagaaattt
     1081 ctacgacccc atcggtgccg cgtaggctac gcgctgcggt tcccggcgcc atacgtcgga
     1141 cgcatcgatg tactgctcca cgccattgcc gtcggagtcc gcgaaataga gcgacttggt
     1201 gacttcgtga tccacaggat gtggcgcgac gctgtccgtc acccggccca acacgtggcc
     1261 taaagccccg atctccatgt gagttgtttc cccttcgaaa tcaaccttgg aatgtatcat
     1321 catcttcaaa ccggcaacat gcgtggccag ccgtgttcat ccccatcccg gacgaatcct
     1381 tcagtaagct tggcagaaac ttgatctgcg ccaccttttc ctcccccaac gtgggggacg
     1441 tggcccgaag ggccggaggg ggaagtgaat gggcacgacg tccccctccg cctcgtggag
     1501 tttatcctcc ggccgcgctt cgcgcggacc ggggggctcg gcaccttccc cacgttcggg
     1561 gagaacgcgt aaagccttgc ccgcgtcctt tcttgcgtgt ttccgcgcgg ctggatcctc
     1621 ccatcatcgc agtatggcta acgtattctg aaaatgcgca ttctcgcatt tacgcgccgg
     1681 taaattgcga ggcgtagact tcccggcgat gggcggacag atcgggatgt ttcgtcagaa
     1741 gctggggtgg actctggctt tttcgaccgc ggtaatcatc gcggcgatgg cgctcgacta
     1801 cgtcatctcc atcgtgatcc tgaaaaatgc cgcgggctat acgccctaca tcacactgac
     1861 gatcagcacc ctcgtggcct ttcccgtcac cttcgccatc gtcagcgcct ggataaacat
     1921 gcggcacgcg cgcgacgagc tctccgtcgc ccatcgcgcg gcagaccaag cccgcgccat
     1981 cgcgctgcag gcattgcggg acgtcgacgt cgcgcggcaa ctcgccgatt cggagcgcac
     2041 cgcggcgctg gaagcgagcc gggcaaaaag cgaattcctc gccaacatga gtcacgagct
     2101 gcgaacgccc ctgaacgcca tcctcggatt ctcggagatg ctgaaaagcg gcatgcgtcc
     2161 cggcaagacc gaggaatatt cggagttcat tcaccgatcc ggccgccatc tcctcagcct
     2221 gatcaacgac attctcgacc tg
//
LOCUS       DASZMC010000287         4512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4252710,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000287 DASZMC010000000
VERSION     DASZMC010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252710"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3132
                     /locus_tag="VG274_05915"
     CDS             <1..3132
                     /locus_tag="VG274_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWE06224.1"
                     /translation="DAKVNLLGFQPVEIIRLLLVFFLAGYFANSWDVLRHARETRASL
                     ARLRIKADELSARSLAPLRERLQGRASEPLVTADQFRQLKPLLSVRTPARFRSSFLLW
                     CALFFAAFFAAHVFLSLRGFAADESLLPATFVLCGIGLILMVSLRDPLRDNLLFADFA
                     QGAVIGCVLLAALATLDFYALFGKLSFVPLIASFLLSVLLVLFGSGPGASDAKVNLLG
                     FQPVEIIRLLLVFFLAGYFANSWDVLRHARETRASLARVTSRFDIPPIEYTLPAVLCV
                     ALSLAFFFLQRDMGPALVFACLFLALYGIARGSAFVPAVGLALVACGFFAGYLIGIPR
                     TVGERVSMFLSPWNNMVHGGDQLAHSLWAYATGGATGMGIGLGDPEIVPAAHTDLILA
                     ALGEEWGFIGIVIVFALYAFLVYRALRIALRARNDYEFFLASGLAAVTALQILLISAG
                     SLGVFPLSGVVTPFLSYGRTSMLANFIVIAILLAISGKSSGSAKVSAAIAPSAIQSRT
                     GLLALAGILAAAGLVVVAKAGYVQVLHSAAIMGDGTLVIQADGARRYQYNPRFQLVMR
                     DIPMGSIYDRNGLPLATSDWAELEKHRADYQQLGIDIDRVCSRAEARHYPFGGLLFDF
                     LGDIRTRTRWGATNTSFIERDSARRLRGYDDRPTLVEVKNPNTHSTERVLRYDYRELV
                     PLLRYRYDPQNPAVRRILDRPRNVKMSIDARLEVQTAQILRKQLQQTNRDKGAAVVLD
                     PATGDLLAAVSLPLPAVDQISGNEANPYLDRARYGLYPPGSTFKVVTAMAAMRKDQSL
                     TQKTYECIRLPDGRAGNFVKGWNRPIRDDVEDKNPHGTVNMERGIIVSCNAYFAQLGA
                     YDVGAQALLDTANMLGIAAAAPNTAAQLKKYLPQSAYGQGEVVASPFQMARVAATVAN
                     GGQMPEGRWITDETNTRTNPPQSVLPAEQAATLAKFMREVVTSGTGRRAAASVPMAGK
                     TGTAELQDADSHAWFIGFAPYGAASSKQSTTPAHHQIAFSVLVENGVYGGTAAAPAAA
                     EIVNAAAKLGLLQ"
     gene            3129..3944
                     /locus_tag="VG274_05920"
     CDS             3129..3944
                     /locus_tag="VG274_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWE06225.1"
                     /translation="MNLFSGIEKSIERQFQRWTERLFGASESNELMLTHRAILAEIET
                     KVEILARGRRIFPYSRVTVTMVSPDANRRAMLETAFGERLESDVREALASAGCEVPAD
                     LAIEVGAVEEGPKPFEIAYAVAEKKPPEPAAAETTKAAPAPTSEPAAPPEPARLITAK
                     GRTQQVEFELTGRRVNIGRLAELTDSEHRVIRRNHIVFDEGADEANATVSRKHAHIRF
                     EEGEYRLCDDESEYGTRVFRDGRSIEVPAGNRRGEKLRPGDEIYLGRACLLFE"
     gene            4103..>4512
                     /locus_tag="VG274_05925"
     CDS             4103..>4512
                     /locus_tag="VG274_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HWE06226.1"
                     /translation="MLVFGLALVVGFTYAGSQLIGDLSQVRGGSIYPFILLGVALFIA
                     LGFEFVNGFHDTANAVATVIYTHSLDPHIAVVWSGTWNFIGVLLSTGTVAFGIISLLP
                     VELILQVGSGSGFYMVFALLTAAIIWNLGTWWFGL"
BASE COUNT          783 a         1643 c         1166 g          920 t
ORIGIN      
        1 gatgccaagg tcaacctgct cggcttccag cccgtcgaaa tcatccggct tctgctcgtc
       61 tttttcctcg ccggctactt cgccaattcc tgggatgtgc ttcgccatgc gcgtgaaacg
      121 cgcgcctccc tcgcgcgcct gcgcatcaag gccgatgaac tctccgcccg cagcctcgcg
      181 cccctgcgtg agcgcctgca gggccgcgcg tctgaaccgc tggttacggc cgaccagttc
      241 cgtcaactca aacctctact ctccgtgcgc acgcccgcgc gctttcgcag ctcttttctt
      301 ctctggtgtg ctctcttctt cgccgccttc ttcgcggcac acgtttttct cagcctccgt
      361 ggattcgccg ccgatgaatc gcttcttccc gccaccttcg tgctttgcgg catcggcctc
      421 atcctcatgg tcagtctgcg cgatccgctg cgcgacaacc tcctctttgc cgatttcgcg
      481 caaggcgcag tcatcggctg cgttttgctg gccgcgctcg ccacgctcga tttctacgcc
      541 ctctttggca agctcagttt cgtccctctg atcgccagtt tcctcctctc cgtgctgctt
      601 gtactcttcg gttccggccc gggcgcgagc gatgccaagg tcaacctgct cggcttccag
      661 cccgtcgaaa tcatccggct tctgctcgtc tttttcctcg ccggctactt cgccaattcc
      721 tgggacgtgc ttcgccatgc gcgtgaaacg cgcgcctccc tcgcacgcgt caccagccgc
      781 ttcgatattc ctcccatcga atacacgctt cctgccgtgc tttgcgtcgc gctctctctc
      841 gccttcttct ttctccagcg tgatatgggc ccggctctcg tcttcgcctg cctgttcctc
      901 gcgctctatg gcatcgcgcg cggcagcgct ttcgtaccgg cggtaggtct cgcgctcgta
      961 gcctgcgggt tcttcgcggg ctatctgatc ggcattccgc gcacggtcgg tgagcgtgtc
     1021 tccatgttcc tctcgccctg gaacaacatg gttcacggcg gcgatcagct cgcgcattcg
     1081 ctctgggcct acgcaaccgg tggcgcaacc ggcatgggca tcggcctcgg cgacccggaa
     1141 atcgttcccg cggcccatac cgatctcatc ctcgccgcgc tcggcgaaga gtggggcttc
     1201 atcggcatcg tcatcgtctt cgcgctctac gccttcctcg tttatcgcgc tctccgcatc
     1261 gctctgcgcg cgcgcaatga ttacgaattc ttccttgcct ccggcctcgc cgcggtcacc
     1321 gcgctccaaa ttctgctcat ctccgccggt tcgctcggcg tctttccgct ctcgggagta
     1381 gtcacgccgt tcctcagtta cggccgcacc tccatgctgg cgaatttcat tgtcatcgcc
     1441 attcttctcg ccatcagcgg caagtcgtct ggcagcgcca aggtctccgc cgcgatcgca
     1501 cccagcgcca ttcaatcccg caccggcctg ctcgcgctgg cgggcattct cgctgctgcc
     1561 ggcctcgtag tcgtggccaa agccggctac gtccaggtgc tccacagcgc cgccatcatg
     1621 ggcgatggca cgctggtcat acaggccgat ggcgcccgcc gctatcaata caacccgcgc
     1681 ttccagctcg tcatgcgcga cattcccatg ggcagcatct acgatcgcaa tggcctccct
     1741 ctcgccacca gcgattgggc cgagctcgaa aaacatcgcg ccgattacca acaactcggc
     1801 atcgacatcg atcgcgtctg ctcgcgcgcc gaagcgcgcc actacccctt cggcggtctg
     1861 ctcttcgatt tcctcggcga cattcgcacc cggacccgct ggggcgccac caacacctcg
     1921 ttcatcgagc gcgattccgc acgccgcctg cgcggctacg acgatcggcc caccctcgtc
     1981 gaagtcaaga atcccaatac ccactccacc gagcgcgtgc tccgctacga ctaccgcgaa
     2041 ctggttccac tccttcgcta tcgctacgac ccgcaaaacc cggcagtccg ccgcatcctc
     2101 gatcgcccgc gcaacgtgaa gatgtcgatc gatgcgcgcc tcgaagtcca aaccgcgcag
     2161 atcctgcgca aacagctcca gcaaaccaat cgcgacaaag gcgccgcggt cgttctcgat
     2221 cccgccaccg gcgatctgct cgccgccgta agtcttccgc ttcccgcggt cgatcaaatc
     2281 tccggcaatg aagccaatcc ttacctcgat cgcgcccgct atggcctcta tccgccgggt
     2341 tccaccttca aagtggtcac cgcgatggct gccatgcgta aggatcagtc gctcacccaa
     2401 aaaacttacg aatgcattcg ccttcccgat ggccgcgccg gcaatttcgt gaaaggctgg
     2461 aaccgtccca tccgcgacga tgtcgaagat aagaatccgc acggcacggt caatatggag
     2521 cgcggcatca tcgtctcttg caatgcctat ttcgcccaac tcggagccta cgatgtcggc
     2581 gcgcaagcgc tgctcgacac cgcaaacatg ctcggcatcg ccgcagccgc gcccaatacc
     2641 gccgcccagt taaaaaaata cctgccgcag tccgcatatg gtcaaggcga agtcgtcgca
     2701 tcgccattcc aaatggcgcg cgtcgccgcc actgtcgcca acggcggtca aatgcctgaa
     2761 ggacgttgga tcaccgacga aaccaacacg cgcaccaacc ctccacagtc agttcttccc
     2821 gccgagcagg ccgccacgct cgcgaaattc atgcgcgaag ttgtcacctc cggcaccgga
     2881 cgccgcgcgg ccgccagcgt ccctatggct ggaaaaaccg gcaccgcgga gctccaggac
     2941 gccgattcgc acgcctggtt catcggcttc gccccctatg gcgccgcttc ttccaaacag
     3001 agcacaaccc cggctcatca ccagatcgcc ttcagcgtct tggtagaaaa tggagtctac
     3061 ggcggaaccg ccgccgcccc cgcggccgcg gaaatcgtca acgccgccgc caaactgggg
     3121 ttgctgcaat gaatctcttt tccggaatcg agaagtccat cgagcgccaa ttccagcgct
     3181 ggaccgaacg cctcttcggc gcctccgagt ccaacgaatt gatgctcacg caccgcgcga
     3241 tcctggcgga aatcgaaacc aaggtcgaaa tcctcgcgcg cggccgccgc atcttcccgt
     3301 attcacgcgt caccgtgacg atggtctcgc ccgatgccaa tcgacgcgcc atgctcgaga
     3361 ccgcctttgg cgaacgcctc gaatccgacg tacgtgaagc gctcgcttcg gcgggttgcg
     3421 aggtccccgc cgatctcgca atcgaagttg gcgccgtgga agaaggtccg aagccgttcg
     3481 agatcgctta cgccgtagcc gaaaagaaac cgcccgaacc ggccgccgcc gaaaccacca
     3541 aagccgcgcc cgcgcctact tctgaacccg ccgcgccgcc cgaacccgcc cgcctcatca
     3601 ccgccaaggg tagaacgcag caggtcgagt ttgagcttac cggccgccgc gtcaacatcg
     3661 gccgcctcgc cgaactgacc gactccgagc atcgtgtcat ccgccgcaac cacatcgtct
     3721 tcgacgaggg cgccgacgag gccaacgcca ccgtctcgcg taaacacgcc cacatacggt
     3781 ttgaggaggg cgaatatcgc ctctgcgacg acgaaagcga gtacggcacg cgcgtttttc
     3841 gcgatggccg ctccattgaa gtgcccgccg gcaaccgccg cggcgagaaa ctgcgtcccg
     3901 gcgacgaaat ctatctcggc cgcgcctgcc ttctcttcga gtaactaccc gaagcgaggt
     3961 aaacgtacag ttacacgaat attaatactg ctgtaacagg cgttatgtag actcacagtc
     4021 cgaggtcaat aactacatgg ctgcccctgc gcctgcaagc gcaatcaatg aaaagctaaa
     4081 caaaagccca ggcaagatag gcatgctcgt cttcgggctc gctcttgtcg tcgggttcac
     4141 ttacgctggt tctcaactta tcggcgatct ttcacaggtg cgcggaggct ccatttatcc
     4201 gttcatcctg ctcggggtcg ccctgttcat cgcgctcggg ttcgaattcg tcaacggatt
     4261 ccacgacacc gcgaacgccg tcgccaccgt catctacacg cactcgctcg atccgcacat
     4321 agcagtcgtc tggtcgggca cgtggaactt catcggggtt ctcctgtcta caggtacggt
     4381 cgcattcggc atcatttctc tgctgcccgt cgaactgatc ctgcaggtcg gctccgggtc
     4441 gggcttctac atggtcttcg cgctgctaac agcggccatc atctggaatc tgggcacgtg
     4501 gtggttcggt ct
//
LOCUS       DASZMC010000288         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2590618,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000288 DASZMC010000000
VERSION     DASZMC010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590618"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VG274_05930"
     CDS             <1..224
                     /locus_tag="VG274_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HWE06227.1"
                     /translation="HRLYELIESLSMEAQDELLALMWTGGPKNQSSFEENLELVQKTV
                     EDNHAFHLAEQCAHLPAYLAEGLKKVNPQ"
     gene            complement(502..861)
                     /locus_tag="VG274_05935"
     CDS             complement(502..861)
                     /locus_tag="VG274_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWE06228.1"
                     /translation="MIELSCHCGAVGLELAAAPETVTDCNCSICRRYGVLWAYYSPKQ
                     VRIVAKDGATDIYMWDDRSIEFHRCKVCGCVSHWAPVDEHLDRMGVNARLMAPEVLAK
                     ARVRRLDGANTGTYLDA"
     gene            1001..1432
                     /locus_tag="VG274_05940"
     CDS             1001..1432
                     /locus_tag="VG274_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE06229.1"
                     /translation="MEIAIRSYADSDSDAVTSIWLSSWQSTGVPAPVTLSELRERWPQ
                     ELAKGWVVHVATVGPDVVGFLALHEDKTEQLFVAPGMQGQGIGKRLLDFAKAARPNGF
                     HLTTALESRAGRFYEREGLTRGETTAHARFGHPIVRYDWRP"
     gene            1488..2216
                     /locus_tag="VG274_05945"
     CDS             1488..2216
                     /locus_tag="VG274_05945"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06230.1"
                     /translation="MTVRVDEVAGSIYRFSTFVPEIAAPAGFTFNQYLVDADEPLLFH
                     CGSRKLFADVSAAVAGVIPLSRLKWISFGHVESDECGSMNQWLAAAPEACVVQGATAC
                     LVSLNDMADRAPRILADGETLDLGGKRVCWIDTPHVPHAWESGLLFEITTRTLFCGDL
                     FTHAGDGPATTTDSIVPSAARAEEMFQSTSLTARTATTIRRMASFAPRTLAVMHGSCF
                     VGDCAQQLHELANFYDERFRASAA"
     gene            complement(2599..3546)
                     /locus_tag="VG274_05950"
     CDS             complement(2599..3546)
                     /locus_tag="VG274_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HWE06231.1"
                     /translation="MLTKYPRASSPARDTIWVDLLDPREDEIVKARADYGIAVPSRQQ
                     LEEIESSSRLRSDRDVLILSMTIASKSDLGESMPTPVGFILTPKLLLTVRYAELHAIA
                     PALERIGKDTAPGSVDMFATLVEAMVDYAADILEQSARKLNAISQRVFKRYGRTREGN
                     FARSNRALKDTLVEVGALGDQLSHIRDSILGLQRIAPYALDKGQKWIGDSVQARLKTV
                     SQDLQSLADFEVHLTDKVQFLLDAVLGFINTEQNDIFKVLTIVSVVGIPPTLIASMYG
                     MNFDTIHEYHWHYGYAWGLFLIVVSALVPTVWFKWRGWW"
     gene            3751..>4068
                     /locus_tag="VG274_05955"
     CDS             3751..>4068
                     /locus_tag="VG274_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06232.1"
                     /translation="MFANRFLLSAALVLAGATTIAAAGDGIRNGDRTTMPGTHARITL
                     VPQSAQSFGRTDLADAGLVTIFSNLAGHYPKGEYWCCEGYNVMGPNSGAGEQWMAAAF
                     TPGA"
BASE COUNT          783 a         1235 c         1256 g          794 t
ORIGIN      
        1 agcatcgcct ctacgagctc attgaatcgc tctccatgga ggctcaggac gagctgctgg
       61 ctctgatgtg gaccggcggg ccgaagaacc agagctcgtt cgaggaaaat ctcgaactgg
      121 tgcagaagac ggtggaggac aatcacgcct tccacctcgc cgaacaatgc gcccatctgc
      181 ccgcctacct ggcggaagga ttgaagaagg tgaacccgca ataggcggcg ccccctcacc
      241 cgcgtttgag cgggcgatgg ccctcgtcct tgagccctct cccgcgaggg gagaggggac
      301 tccgcgcgct gattttccgg gtttgatgcg agattccgat tctcatcccc cttgccggct
      361 tcgccaaagc tacggcgggt cgtgcggctc atttggtccg ccgaagcctt ggcgtaggcg
      421 gacgggagag ggacgcagat acgtgcaatg atttttgctc gggcgtgggt gaggggtgcg
      481 gccaacgcgc cgcgtcgtcc atcacgcgtc aaggtatgtc ccggtattgg cgccatcgag
      541 gcggcgcacg cgcgccttcg ccagcacctc cggcgccatc agcctggcgt tcacgcccat
      601 gcgatcgaga tgctcgtcca cgggggccca atgggagacg cagccgcaga ctttgcaacg
      661 gtggaactcg atcgacctgt cgtcccacat gtaaatatcg gtcgcgccgt ccttcgcaac
      721 gatgcggacc tgcttcggcg aataataggc ccacaacacg ccatagcgcc ggcagatgga
      781 gcaattgcaa tcggtcaccg tttccggcgc cgcggcaagc tcaagcccga ccgcgccgca
      841 atgacaggac aattcgatca tgatggactc cataacggcg tatcgatcgt ctcgatttcc
      901 gtcacgcccg tcatctttgc ccccgcatcg cctactctaa tatagggaaa ctgccgcccg
      961 gtctgtcaaa gcgcttgaac cacgaaaagc gggatgcctg atggaaatcg ctatacgttc
     1021 ctacgcggat tcggattctg atgcggttac gtcgatttgg ctaagcagct ggcaatcgac
     1081 tggcgttcct gcgcccgtaa cgctgagcga gttgcgcgaa cgatggccgc aggaactggc
     1141 aaagggttgg gtcgtccatg tcgcgaccgt ggggccggac gttgtcggat ttctcgcgct
     1201 tcatgaagac aaaacggaac agttgtttgt tgcccccggc atgcagggac agggtatcgg
     1261 aaaacggctt ctggattttg cgaaggccgc cagaccgaac gggtttcacc tcaccaccgc
     1321 actcgaaagc agggccggcc gattctacga acgggagggg ctgacgcgcg gcgaaacgac
     1381 ggctcatgcg cggtttgggc atccgattgt tcgatacgat tggcgcccat gatttcaaaa
     1441 tccgcaatgc ccggctatgc tgcgggatcg gtgcggggag actccgaatg accgttcgcg
     1501 tggacgaggt tgccggcagc atctatcgat tttcgacctt tgtgcccgaa attgccgctc
     1561 cggcggggtt cacgttcaat caatatctgg tcgacgcaga cgagccgctg ctgttccatt
     1621 gcggctcgcg aaagctgttt gccgatgttt ccgccgcggt agcaggcgtc attccgcttt
     1681 cccggctcaa gtggatttcc ttcggccacg tcgaatccga tgagtgcggt tcgatgaatc
     1741 aatggctggc ggcggcgccc gaggcatgcg tggttcaggg ggcgacagcc tgcctcgtct
     1801 cgctgaacga tatggccgac cgggcgccgc gaattctggc cgatggcgag acgctcgatc
     1861 tcggcgggaa gcgtgtctgc tggatcgata caccgcacgt cccgcatgcg tgggagtccg
     1921 gcctgctgtt cgagattacg acgcgaacgc ttttctgcgg cgacctcttc acgcatgcgg
     1981 gcgacgggcc ggccacgacg acggacagca tcgtcccctc tgccgcccgg gccgaagaga
     2041 tgtttcagtc gacgtcgctt acggccagga ccgccacaac gatcaggcgt atggcttcgt
     2101 tcgcgccccg cacgctcgcg gtcatgcatg gctcgtgctt cgtgggagac tgcgcgcagc
     2161 agctgcacga gctggcgaat ttttacgacg aacggttccg cgccagcgcg gcctgattgc
     2221 gcggccgccc cctccgcccc ttccataggc gccaagataa gctcgctgga ccaaaaaagc
     2281 gtttcattcc cgcgccaccg gtccgcgcga agcgcggccg gagcgtaaac ttcagcggga
     2341 acccagctcg taaagcgcag aaggttttgc gcggcgccga aatcatcttg tcgctgctgc
     2401 ggacgctggg ttcccgcttt cgcggaaatg acacgccgtg gggacgcgaa aatgataaat
     2461 ttggttgggg taaaccgcct atcctagcgc ctacgcaagc gattcaattc gcctgcaatc
     2521 aagccgtgct ttcgaatccg cgaattaatt taacccacgt tcgatcgtag aggcgcgcac
     2581 gatcccgcca actcgcgttc accaccagcc gcgccatttg aaccagacgg tgggcacgag
     2641 ggcggagacg acgatcagga acaggcccca ggcatagccg tagtgccagt gatattcgtg
     2701 aatggtgtcg aaattcattc catacatgct ggcgatcagc gtcggcggaa tgccgaccac
     2761 cgagacgatg gtgagcacct tgaatatgtc gttctgctcg gtgttgatga agcccagcac
     2821 cgcatcgagc aggaactgca ccttgtcggt gagatgaact tcgaagtcgg cgagcgattg
     2881 cagatcctgg ctcaccgtct tcaggcgggc ctggacggaa tctccgatcc atttctgccc
     2941 cttgtcgagc gcatagggcg cgatgcgctg caggccgagg atgctgtcgc ggatatggct
     3001 cagctgatcg ccgagcgctc cgacttccac cagagtatcc ttgagcgccc ggttggatcg
     3061 cgcgaaattg ccctcgcggg tgcgcccgta acgcttgaac acgcgctgcg agatcgcatt
     3121 cagcttcctg gcactctgtt ccagaatgtc ggcggcgtaa tcgaccatcg cctcgaccag
     3181 cgtggcgaac atgtcgacgc tgccaggcgc cgtgtccttc ccaatcctct ccagagccgg
     3241 cgcgatcgcg tgcagttccg catagcgcac ggtgaggagc agcttcggtg tcagaatgaa
     3301 gccgaccggc gtcggcatcg attcgcccag atcggatttc gaggcgatcg tcatgctcag
     3361 gatcagtacg tcgcgatccg agcgcaaccg gctcgaggat tcgatctcct ccagctgctg
     3421 ccgcgacggc acggcaatcc cgtaatccgc gcgcgctttc acaatctcgt cttctctcgg
     3481 gtcgagcagg tcgacccaga tcgtgtcgcg ggcgggcgaa cttgcgcggg gatatttcgt
     3541 cagcatcggc ggcgcctgtc gggggcgagt cgatgacaat cttactcccc ttgggaggga
     3601 ggtcgaaatt tgcaggcaaa tttcgggtgg ggcctgtgcg acgcgcgttt acctcagagc
     3661 ctcatcctga gcttgtcgaa cgatgaaaac cgaatcatat ttcagcgata gcgaatgcgc
     3721 aggaaatgcc cagtcgcaag ggagagacag atgtttgcaa acagatttct gctgtcggcg
     3781 gcgctcgttc tggctggcgc gacgacgatc gcagccgccg gggacggcat caggaacggc
     3841 gaccgaacaa ccatgccggg cacgcatgcg aggatcaccc tggttcctca atcggcgcaa
     3901 tctttcggca gaaccgatct cgccgatgcc gggctggtga caatattcag caatctcgcc
     3961 gggcattatc cgaaagggga atattggtgt tgcgaaggct acaacgtcat gggcccgaat
     4021 tccggcgcgg gcgagcaatg gatggccgcc gccttcacgc ccggcgcg
//
LOCUS       DASZMC010000289         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_934942,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000289 DASZMC010000000
VERSION     DASZMC010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934942"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1765
                     /locus_tag="VG274_05960"
     CDS             <1..>1765
                     /locus_tag="VG274_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06233.1"
                     /translation="LARALRGQEGEALVDQAWRTPGVRDLVTIPLYLTALLRSTPGAR
                     FPQTKEEVLRLFVTQHEEEPEKAAILQKELFGFHSDMLTGLGVEANRAANTVLADTSA
                     NRVIANIGAQLAAAGQFTNPPQPAAAIEVLVSSHLLIRASSGGISFQHHQFQEWYASL
                     DVGRIMREAANGNAEARTKLRANILNWPAWEESVLFACERLSRENAGGAQAVATAIRE
                     TLGIDPMLAAEMIFRSAPEVWTLTGAETTAFAARWHTAGKVDRAARFLMTTGRPEFAP
                     QIWPLVSNPDNQIYLAALRSPQRFRPSVLGDDAEQKLAALPVETRKHVVAEIAGHSGF
                     DGMELAIRVAKADPSADAVLEVLQALQFRRADRMVTEILQTASDEVWQLVSRAGYPNE
                     LADPEQNKKLDELRRTQIETETDPVNSLRSLAARQVKDAETETRIEELIASTGFPARN
                     DQAAHVLQEAQAAYPGPVRKGLQRRIATRLELPFRAREFLTGGDALDEGPVVEALLDA
                     GAPELVTRAGFVVVGPKTVGAVIDRLFELHEAYHRDRDAWGGAGHQAEREELGRLRTA
                     IEASREDSFLTALLERAQTDDP"
BASE COUNT          349 a          553 c          564 g          299 t
ORIGIN      
        1 actcgcgcgg gcattgcggg ggcaggaagg cgaagcgctg gtcgatcagg cctggcgcac
       61 gccgggcgtg cgcgatctcg tgaccatccc gctctacctg acggcgctcc ttcgcagcac
      121 gccgggtgcg agatttccgc aaacgaaaga agaggtccta cggctcttcg tcacgcaaca
      181 cgaagaagag cccgagaagg cggcgatcct gcaaaaggaa ttgttcgggt ttcacagtga
      241 catgctgacc ggcctcggcg tcgaggcaaa ccgcgccgcg aacacggtgc tcgcggacac
      301 gtccgccaac cgggtcatcg cgaacatcgg cgcgcaattg gccgcagcgg gtcaattcac
      361 aaacccgccg cagcctgcgg ctgcaatcga ggttctggta agcagccatc tcttgatccg
      421 cgcctcgtcc ggcggcattt cgttccagca ccaccaattc caggaatggt acgcgtcgct
      481 cgatgtaggc cggatcatgc gagaggcagc gaacggcaac gcggaagcgc gaacgaagct
      541 tcgagccaac attctgaact ggcccgcttg ggaggaatcg gtactattcg cctgcgagcg
      601 gttgtcacgc gagaatgctg gcggcgcgca ggccgtcgcg acggctatcc gcgagacgct
      661 ggggatcgac cctatgctgg ccgccgagat gatcttccgc tcggcgcccg aggtttggac
      721 gctgaccggc gccgaaacca ccgccttcgc ggcgcgctgg cacacggccg gcaaagtgga
      781 tcgcgcggcg cgtttcttga tgacgaccgg ccggccggag ttcgcgccgc aaatctggcc
      841 gttagtctct aacccggaca accaaatcta tctcgccgct ttgcggtcgc cccaacgctt
      901 tcggccatcg gtcttgggcg acgatgccga gcaaaagctc gccgccttgc ccgtcgaaac
      961 gcgcaagcat gtcgtcgccg aaatcgctgg ccacagcggc ttcgatggca tggagctggc
     1021 aatccgcgtg gcgaaggccg atccgagcgc agatgccgtg cttgaagtgc tccaggcact
     1081 gcaattccgg cgcgccgacc gcatggtgac agaaattctg cagaccgcgt cggatgaagt
     1141 ctggcagctt gtttcccgcg ctggatatcc caatgagctc gccgatcccg aacagaacaa
     1201 aaagctcgac gaactccggc ggacgcagat cgagacggaa accgatcctg tgaatagcct
     1261 gaggagcctt gccgcgcgtc aggtcaagga cgcggaaacc gaaacgcgga tcgaggaact
     1321 gatcgcgtca actggttttc cagcaaggaa tgatcaagca gcgcacgttc ttcaggaggc
     1381 acaggcggcc tatcccggac cggttcggaa agggcttcag cgccgcattg ccacgcgctt
     1441 ggaattgccg ttccgtgcac gcgagttcct gacgggcggc gatgccctcg acgagggtcc
     1501 tgtcgtggaa gcgttgctcg atgcgggcgc ccctgaattg gttacacgag ccggcttcgt
     1561 cgtcgtcggt cccaaaaccg tcggagcggt gatcgaccgg cttttcgaac tgcacgaagc
     1621 ctatcatcgt gatcgcgacg cgtggggagg cgccggacat caggccgaac gggaggaatt
     1681 gggacggttg cggaccgcca tcgaagcttc ccgcgaagac tcgtttctga cggctctctt
     1741 ggaacgcgcg cagaccgatg atccc
//
LOCUS       DASZMC010000290         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5343246,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000290 DASZMC010000000
VERSION     DASZMC010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5343246"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VG274_05965"
     CDS             <1..353
                     /locus_tag="VG274_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWE06234.1"
                     /translation="IAAPMYNFTISSTLKAWIDHITRAGRTFGYTAQGPEGLLKNKKV
                     FVVTARGGIYSGDSAMKPYDFQEPYLRTILGFLGMTDVTFIHTEGLNISTDAAEAGVA
                     RARAAIADVIPVAA"
     gene            481..1470
                     /locus_tag="VG274_05970"
     CDS             481..1470
                     /locus_tag="VG274_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HWE06235.1"
                     /translation="MENGVFLIERFGLFAIFVNVLLSSGGLPLPCYPMLMLAAALAAP
                     TGIRIPEIVATGAVATLIADSAWFWGGRRYGGTVLKLLCRISLSPDSCVRRTETVFTR
                     VGPASVALAKFVPGLSNISVALAGATGIAWPTFLLFDAAGAVGFIGAGVALGTFFRRA
                     IAAGLGALDRLGEWGIVFIVAALSAFLVWKWLQRQWFLRQLRVDRVTVAELRAMIEAG
                     TRPTILDVRSAESRARGARIPGSIAADALEQIRPSLSRDVEIIVYCACPNEASAALLA
                     NELRRAGFRSVRPLLGGIDAWVEAGLATEAGAKPLTSKCSKPCIDGPNMAQAA"
BASE COUNT          269 a          502 c          484 g          299 t
ORIGIN      
        1 tgatcgccgc ccctatgtac aatttcacca tttcgtcgac gctcaaggcc tggatcgatc
       61 acatcacgcg cgccggacgg accttcggct atacggcaca gggacccgag gggcttctca
      121 aaaacaagaa ggttttcgtg gtcaccgccc ggggcggaat ctacagcggc gacagcgcaa
      181 tgaagcctta cgatttccag gaaccgtatc tgcgcacgat cctgggattt ctcgggatga
      241 cagacgtcac cttcattcac accgaggggc tgaacatcag caccgacgcg gcggaggccg
      301 gcgtggcccg cgctcgcgcc gcaatcgccg atgtcatacc cgtcgccgcg taaattccga
      361 tcctgaagag ctccaggtac gagcactcag cggcgcatgc agtgccggta tgaaaaaaca
      421 gcccttctgc cgcagcgttt gtacacgaaa taaggcttca gcaaggaggg agccctcgcc
      481 gtggagaatg gcgtttttct catcgagcgc tttgggctgt ttgcgatctt cgtgaacgtg
      541 ctgctgtcgt cgggcgggct tccactgccg tgctatccga tgctgatgct ggcggcggct
      601 ctcgcagcgc ctacgggcat tcgcatcccg gaaatcgtgg cgacgggcgc ggtagcaacg
      661 ctcatcgccg attccgcgtg gttctggggc ggccggcgct atggcggcac ggtgctgaaa
      721 ctcttgtgcc gcatctcgct ctcgccggat tcctgcgtcc gcagaaccga gaccgtcttc
      781 accagggtag gtcccgcatc agtcgccctg gcgaaattcg ttcccggcct gtcgaacatc
      841 tcggttgcac tcgcaggcgc cacgggcatc gcctggccga ccttcctgct tttcgatgcc
      901 gcaggcgccg tcggcttcat cggcgccggt gtcgctctgg gcactttttt tcggcgtgca
      961 attgcagccg gtctcggtgc gctggaccgg ttgggagaat ggggtatcgt cttcatcgtt
     1021 gccgcactgt ccgccttcct tgtatggaaa tggctgcaac gccaatggtt tctccggcag
     1081 ctgcgcgtcg accgggtcac ggttgccgaa ctgcgcgcaa tgatcgaggc gggcacgcgc
     1141 ccgacgatcc tcgatgtgcg ctcggccgaa tcccgggcgc gcggcgcgcg gatcccgggc
     1201 tcgatcgccg ccgacgcgct tgaacagatt cgcccctcgc tctcccgcga cgtcgagatc
     1261 atcgtctatt gcgcctgccc caatgaagca tccgcagcgc ttctggcgaa cgagctgaga
     1321 cgtgccggat tccgaagcgt gcgcccgctc cttggaggaa tcgacgcctg ggtcgaagcc
     1381 ggtctggcaa cggaagcagg cgcgaagccc ttgacctcca aatgcagcaa gccctgcatc
     1441 gacggcccga acatggccca ggcggcataa cagcgcgtag cgcgcgaacg cgtcggactt
     1501 tcctccccca acgtggggga ggtggtttgc gcagcgaagc ggagcaaacc ggag
//
LOCUS       DASZMC010000291         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5057702,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000291 DASZMC010000000
VERSION     DASZMC010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5057702"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VG274_05975"
     CDS             <1..738
                     /locus_tag="VG274_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06236.1"
                     /translation="NDLGVAPCTLFYRRRVLDMPLSDIAPDAPPKRAEAMRQARIKIL
                     DKARRKFLFDFHERSVPTGLIVENGRLTGIQMSRTEVADGKVRTLPDTREPVHGALTV
                     SSIGSIPEPIPGIPQRGEVYEYADQQTGLLIEGDTAIYAAGNVLTGKGNIKDSLESGT
                     AIGTHVAEQYLGLADDGGRLALTEGARENAHIEGLRIAGGIMAREPLPPGRAAEIMRR
                     VSDRQKKVGYDGDYRTWIAKVTPLDLQ"
     gene            797..1066
                     /locus_tag="VG274_05980"
     CDS             797..1066
                     /locus_tag="VG274_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06237.1"
                     /translation="MNDSIREISDMRIKGIDPENAPDSLKPVFQRSLDLFGRVITPNL
                     VMAHRPEILLATAKLGQAIGASSVMEGRLKSMASLRAAQMIGCPF"
     gene            1118..1402
                     /locus_tag="VG274_05985"
     CDS             1118..1402
                     /locus_tag="VG274_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06238.1"
                     /translation="MENLAAFEASAHFDRREKAVLRYAEGMTRTPADVSEADFNEIRA
                     LFSTEQIVELTAAIALENFRARFNCALKIGSDDLCTLPPDHPVRKALERP"
BASE COUNT          346 a          473 c          525 g          293 t
ORIGIN      
        1 aacgatctcg gcgtggcgcc atgcacattg ttctaccgcc gccgcgtgct ggatatgccg
       61 ctgtcggata tcgctcccga cgcgccgccg aaacgggccg aggcgatgcg ccaggcgcgg
      121 atcaaaattc tcgacaaggc gcggcgcaaa ttcctgttcg atttccatga acggagcgtg
      181 ccgaccggat tgatcgttga gaatggccgc ctgacgggca ttcagatgag ccgtaccgaa
      241 gtcgccgacg gcaaagtccg cactcttcct gacacgcgcg agccggttca cggcgctctg
      301 acggtcagct cgatcggcag catcccggag ccgattccgg ggataccgca gcgcggcgag
      361 gtttacgaat acgccgatca gcaaacaggg ctgctgatcg agggcgacac agcaatctat
      421 gccgccggca atgtgctgac cggcaaaggc aatatcaagg attcgctcga aagcggcacg
      481 gctatcggca cacacgtggc cgagcagtac cttggactgg ccgatgatgg cgggcggctc
      541 gccctgaccg aaggcgcgcg cgagaacgcg cacatcgaag gtttgcgcat cgcgggcggc
      601 atcatggcgc gggaaccgct gccgccgggg cgggcggcgg aaatcatgcg gcgcgtgagc
      661 gatcgtcaga aaaaggtcgg ttatgacggc gattaccgca catggatcgc caaagtgact
      721 ccacttgact tgcagtaacc cgtgaattgc aactcgtcgc cgttcggtcc ggcggcgaaa
      781 ttgccatgtg atattcgtga acgattcgat ccgggagatt tcggatatgc gcatcaaggg
      841 aatcgacccc gaaaacgcgc cggatagctt gaaaccggta tttcagcgca gtctcgacct
      901 gttcggccgg gtcatcacgc cgaatctcgt gatggcgcat cggcccgaaa tcctgctggc
      961 gacggcgaag ctggggcagg cgatcggcgc ttcaagcgtc atggaaggcc ggctcaagtc
     1021 aatggcatcg ctgcgcgcgg cgcaaatgat cggctgcccg ttttgagtgg acatcaactc
     1081 tgccgtgggc agggaaagcg ggattacgga tcggcagttg gagaatttgg cggcgtttga
     1141 ggcaagtgcg catttcgacc gccgcgaaaa ggcggtgctg cgctatgcgg agggcatgac
     1201 gcggactccg gccgacgttt cggaggcgga tttcaacgag attagggcct tgttcagcac
     1261 ggagcaaatc gtcgaactga cggcggcaat cgctctggag aatttccgcg cccgattcaa
     1321 ctgcgcgctc aaaatcggga gcgacgacct atgcacgctg cctcccgatc atccagtccg
     1381 caaagcgctc gagcgcccat gacgatcgca atcgtcgtgg gaacgccgcg cctgctgaac
     1441 aggcaaaatc tcgtccgcct gtcaccgttt ttcatggaaa ccgtggggcg cgccgcccga
     1501 ttttgatcca agccgctctc ggcggtagtc tgaaggcttg aacttgcggg agtatgccgt
     1561 ggaccaactg aagcgaaccg accccgagat ttacgagctg atcaaggaag aggagcgata
     1621 cgaaatcgat tcggttc
//
LOCUS       DASZMC010000292         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1505343,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000292 DASZMC010000000
VERSION     DASZMC010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505343"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VG274_05990"
     CDS             <1..657
                     /locus_tag="VG274_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06239.1"
                     /translation="NAALAQGNAAGYALAARSALAAEMMRPEPCMACLEHAETLAQHA
                     IDADPKVVEGQIEFVVALGYRARLMGMVEAHLKGLAKIARNHIDAALADDPGNAWAWA
                     ALGGWNIEIARGAGSALARWLYGASLRAGLDDFSRAFQAAPDNLVLRYQYALSLAAYD
                     RDAYRRPILDALSRAAVDQPQSAYETFAQRNVKELLGALKSGDLQGFDRMVRHDQGYR
                     "
     gene            668..1720
                     /locus_tag="VG274_05995"
     CDS             668..1720
                     /locus_tag="VG274_05995"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HWE06240.1"
                     /translation="MLIENGAGLLRKLPPEAAHRATLALVGLSAPFLGRACPDDPRLA
                     LNALGLNFPNPVGLAAGFDKDARVPDAMLHRGFGFVECGTVTPLPQPGNPRPRLFRLE
                     SDLAVINRMGFNNAGARRVARRLAARARRGILGINIGANKDSVDRIADYRAAFDMLAP
                     FSDYVSINVSSPNTPGLRALQGGEELARLLGLLVSERGSRNLRIPLLLKISPDLELRE
                     LDVIADVASASGIDGLIVSNTTVARPDYLTSAYAREDGGLSGAPLFDRSTRLLSETRA
                     RVAGRLTLIGVGGISSGATAYAKIRAGATLLQLYTALALQGPGLLKRIKEELLALLSA
                     DGFSNIMEAIGRDTSA"
     gene            1824..>2939
                     /locus_tag="VG274_06000"
     CDS             1824..>2939
                     /locus_tag="VG274_06000"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWE06241.1"
                     /translation="MNWTRIVFVAVIALSAPAMAQSGSDVEQVVVTATRLPERMDQVP
                     ADISIVSGAELKARDAWDMATSLGLVPGVEAPSGGDAGPSSAVPSFWGLHEFDAFLLV
                     VDDIPWGGAFNPAISTLDLTDVQRIEVLKGAAPVMYGATSFVGVVQLLHYPAGEATNE
                     GDIAFGNYGSSRGSAAFALPQFDNYRQSLAFDGQGRGFADKREAVDDGQFLYRGALDL
                     GGGTLRVDAGITVVRDVPPSPIIRVGSALTTLTPINANFNPADSRIDENKYQLAIGYS
                     VPTAWGDWSTLVSSAHSDIVDIRAFLHPDLSGTADTQNQHRLINDDYFDTHLSSTFFG
                     DTTLIVGMDLLHGLGRQTSDNGNGAYTVPLNGSVLPPP"
BASE COUNT          513 a          932 c          914 g          580 t
ORIGIN      
        1 aatgcagcgc ttgcgcaggg caacgcagcg ggatacgcgc tcgccgcgcg ttcggcgctg
       61 gcagcggaaa tgatgcggcc ggaaccctgc atggcctgcc tcgaacacgc agagactctg
      121 gcgcagcacg caatcgacgc ggatccaaag gttgtcgagg gccagatcga gttcgtcgtc
      181 gcactgggct accgggcgcg tctcatgggc atggtggagg cccatctcaa gggacttgcg
      241 aagattgcca ggaaccacat cgacgccgcg cttgccgacg atcctggaaa tgcctgggct
      301 tgggcggcgc tcggcggctg gaacatcgag atcgcccgtg gcgccggttc cgccctcgcc
      361 cgttggcttt acggcgcaag cctgcgggcc ggcctggacg acttttcaag ggcgttccag
      421 gcggctccgg acaatctcgt gctgcgctat caatacgcgc tttcgctcgc cgcctacgat
      481 cgtgacgcct atcgccgccc gattctcgat gctctgagcc gcgcagcagt ggaccaaccg
      541 caaagcgcct acgaaacctt tgcgcagaga aatgtgaagg aattgctcgg cgcgctgaaa
      601 tccggagacc tgcagggctt cgaccgcatg gtccggcacg accagggcta tcggtgatcg
      661 cgaacgcatg ctgatcgaaa atggcgccgg tcttctgcgc aaattgccgc cggaagcggc
      721 gcatcgtgca acgctggcgc tggttggtct gtcggcccca ttcctcggtc gcgcatgtcc
      781 cgacgatccc cggctggccc tcaatgcgtt gggcctgaac tttccaaatc cggtcggcct
      841 cgcagccgga ttcgacaagg atgcgcgcgt tcccgatgcg atgctgcacc ggggtttcgg
      901 tttcgtcgaa tgtggtacgg tgacaccgct tccgcagccc ggcaatcccc ggccccgcct
      961 cttccgtctc gaatccgatc tggcggtcat caatcgcatg ggatttaaca atgccggcgc
     1021 acgccgcgtt gcccggcggc ttgcggcccg cgcgcgtcgg ggcatcctgg gcatcaatat
     1081 cggagcgaac aaggacagcg tcgaccgcat cgcagactac cgcgccgcct tcgatatgct
     1141 cgcgccgttc tcggactatg taagcatcaa tgtttcatcg cccaatacgc cgggcttgcg
     1201 cgccctgcag ggtggcgaag agcttgcgcg ccttcttgga ctcctcgtgt cggaacgcgg
     1261 ctcgcggaat ctccgcatac ctctgctctt gaagatttcg ccggatctgg aattgcgcga
     1321 gctggacgtg attgcggacg tggcgtctgc ttccggcatc gacgggctga ttgtctcgaa
     1381 cacgacagtc gcacgtccgg actacctcac gagcgcttac gccagggagg acggcgggtt
     1441 gtccggagcg ccgctgttcg atcggtcgac gcgactcctc agtgagaccc gggcacgggt
     1501 cgcgggacgg ctcacgctga ttggcgtggg aggaatctct tcgggcgcta cggcctatgc
     1561 gaagattcgc gcgggcgcca cgctgctgca gctttacacg gcgctggcgc tacaaggccc
     1621 gggcctgctg aagcgcatca aggaggaatt gctcgcgctg ctttcggctg atggtttctc
     1681 gaacatcatg gaagcaatcg gtcgcgatac atcggcctga ttctgccagc ctcttgccaa
     1741 attccgacaa tgcgagttag tcgcagtcgc gagcggaagt caaaggctga ctggcgcgaa
     1801 cggggcgtgc caggaaaaat ccgatgaatt ggacacggat cgtatttgtc gccgtgatcg
     1861 cgttatcggc cccagccatg gcgcagagcg gctcggacgt cgagcaggtg gtcgtgactg
     1921 cgacacgcct tcccgagcgc atggaccagg tccccgccga catttcgatt gtgtccggag
     1981 cggagctcaa ggcgcgcgat gcgtgggata tggcgaccag tctcggcctt gttcccgggg
     2041 tggaggcgcc ttccggcggc gatgccggac cgtcgagcgc ggtaccatcc ttttggggtt
     2101 tgcatgaatt cgatgctttc ctgttggtcg tcgacgatat cccctggggc ggagccttca
     2161 atcctgcgat ctccacgctc gatcttaccg acgtgcaacg cattgaagtc ctgaagggcg
     2221 cggctcccgt catgtatggc gcaacctcct tcgtcggtgt cgtgcagctg ctccactacc
     2281 cggccggaga agccacgaac gaaggcgaca tcgcattcgg gaactacgga tcgtcgcgcg
     2341 gctctgccgc cttcgcgctt cctcaattcg acaattaccg ccagtcgctt gcgttcgacg
     2401 gacaaggacg cggattcgcg gataaacgcg aggctgtcga tgacggtcag ttcctttacc
     2461 gcggcgcact cgacctgggt gggggaacgc tgcgtgtgga tgccggcatc acggtggttc
     2521 gcgacgttcc gcccagtccg atcattcggg tgggttccgc attgaccacc ctcacgccga
     2581 tcaacgccaa tttcaatccc gcagactcgc ggatcgacga gaacaaatat cagcttgcga
     2641 tcggatattc cgtgccaacc gcctggggcg actggagtac gcttgtgtcc tccgcgcatt
     2701 cggacatcgt cgatatccgg gccttcctgc atcccgatct gagcggcacc gcggataccc
     2761 agaatcagca ccgtctcatc aacgacgatt atttcgacac gcatctttcc agcacgttct
     2821 ttggcgacac gacgctgatc gtgggcatgg atctgctcca cggcctcgga cgccagacgt
     2881 ccgacaatgg caatggcgcg tataccgtgc cgctcaacgg ctcagtgctt cctcctccg
//
LOCUS       DASZMC010000293         6528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3972764,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000293 DASZMC010000000
VERSION     DASZMC010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3972764"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VG274_06005"
     CDS             <1..777
                     /locus_tag="VG274_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06242.1"
                     /translation="SDFFALRRPSASINYIASHDGATLADLTMYEGKHNEANGEDNRD
                     GVSDNLARNWGVEGPGDDATINAMRARMRRSLLATLFWSRGVPMLLAGDEFGRTQNGN
                     NNAYCQDDEISWLDWSKADESLIAWTARLTALRRVYLGLHEDRFRHGQEVIRGFRDLD
                     WLDERGKILSEEDWQNAEGRALAMRRTTKRADGKVEIIALLMNSSEDALEFTLPDGFD
                     WRVIFDSAEPEREADALRKKSCKVESHACVMTAAVVEAPS"
     gene            774..2381
                     /gene="treZ"
                     /locus_tag="VG274_06010"
     CDS             774..2381
                     /gene="treZ"
                     /locus_tag="VG274_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HWE06243.1"
                     /translation="MTRFRLWAPACTHVGLDVTGNVITMPQIMDGWFDVEADASVGAR
                     YRFLIGEQAVPDPASRLQNEGWSVVTDPDAYPWTDGSWRGRPWEETILYELHVGLMGG
                     FQGVEDRLAALAELGITAIELMPIAQFPGIRNWGYDGVLPYAPARAYGSPDDLKRLVD
                     RAHALGLMVFLDVVYNHFGPDGNYLPLYAPDFFREDRHTPWGAGIDFRKEQVRRFFID
                     NALYWVNEFHIDGLRLDAVHAIDDNEFLVELVDSVRAAAPERYVHVVVENERNDAKLL
                     AEGGIGQWNDDFHHAVHVLLTGESEGYYSEYADAPAQGLATSLSGKFIRERSSPGPEL
                     APTAFVNFLQNHDHVGNRAFGERLIALANEDALRAAIALLLLAPPIPLIFFGEETGAR
                     EPFLYFCDHVDAKLADAVRKGRRNEFTKFPEFQDPEKRAQIPDPNAPETFERSRPHLD
                     DGAEWRAFYKELLDLRRTHITPRLKGCTAEGAEVVGPKAVCATWRMGDGTRLTILTNL
                     DRVPPSYVLPSAKPLWDRAGTRCWIEP"
     gene            2378..4063
                     /gene="malQ"
                     /locus_tag="VG274_06015"
     CDS             2378..4063
                     /gene="malQ"
                     /locus_tag="VG274_06015"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWE06244.1"
                     /translation="MMADTALYDRARKAGLAPDWIDAAGAHKTVSPDTLRVVLDALDG
                     FEFSDTQPDGSTTCFSVGEAVPGRRVAALAVQLYALRGSNGFGDLGALARFAREAAPF
                     GIDALAVSPIHAPFLAVADDISPYSPSSRLFLNPLYAAEEVPDDGVEGLVDWPQASRA
                     KIERLREKFANAPPVHAEGRLRDHARFEALDAHFRARKLYRWQDWPSAYRNPRGAEVE
                     AFAREHHAEVDFHIFLQALTELSLANAHAAARDAGMAIGLIADIAVGMSPHGSHAWSA
                     PSDVLRGLTIGAPPDIFNPQGQNWGLTALSPGAPREAFADTWDAAMRYAGGVRIDHAM
                     GLKRLWVIPDGAGPHDGVYLHYPMHNLFALLAERSRHHRAIVIGEDLGTVPDGFRDAM
                     HSAGMLGMEVLWFQRDGRRFLPSERWSKHAAALTTTHDLPTVTGWWGGRDIDWLEKLG
                     RKSEHGSIDAERWARDEDRAHLWSVIGHGLAPSPDETERVLDAAMGFIGRTPCEIAIV
                     PVEDVLDVVEQPNIPGTIDEHPNWRRRLPPGDSLAGAAARANLEALVRERSRP"
     gene            4060..6465
                     /gene="treY"
                     /locus_tag="VG274_06020"
     CDS             4060..6465
                     /gene="treY"
                     /locus_tag="VG274_06020"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HWE06245.1"
                     /translation="MTPRATYRMQFHKDFTFADATALAPYLSALGISHLYSSPILAAR
                     AGSMHGYDVIDHARINPELGGEDGFRALAAALRAHDIGIVLDIVPNHMAVGGADNSCW
                     LDLLEKGRDSVFANMFDIDWEPAQPNLRDKVLVPLLGSKPRDAIAADEISLIWDEGLG
                     KYAFAYAEHRFPLCREDYAEVEADLAAANAPETLSELLERQNFRLAFWRDAGERINWR
                     RFFDITELAALRVEDDGVFEIVHATTFRLYGEGLIDGVRVDHVDGLSDPKAYCQRLRA
                     RLEALNAQRERKEAPYIIVEKILAQDESLPEDWGIDGTTGYDFMDQISALQHDGANAE
                     RFAKLWSEISGRSAEFEPEERTARREVMESAFANQLRGAAMAFQALAPDIGQEDFEAA
                     LAVLLENFHAYRTYTGTPSPPFDAAYRAARAGAPRAALNAIANAICSSNEAVRDAVRR
                     FNQLAAPVAAKAVEDTAFYRYGRLLSRNDVGFAPDRFAISNEEFLRKAAHRGQLFPRA
                     LLATATHDHKRGEDVRARLAALSEIPDVWEQEVRAWFAVNAPLRPSQVTAGDEYQLYQ
                     TLVGAWPIETERVLAWREKSLREAKLQTSWFAPNSEFEKENAAFVQAILDPSHDFVGR
                     LDHFAAKLSPAGICNSLVQCALRYTLPGIPDLYQGSELGDFSLVDPDNRRPVDYTLRQ
                     ELLSAPVSLDGWQMGTPKLGLIRRLLALRRERPEVFDADMECVTASRHLLVFRRQSLL
                     VAVPLLSSYSCMERGVPLPIVTGTLHPEGQWRNALDPSLSRVRSLDCAELFSTFPVAV
                     LVS"
BASE COUNT         1180 a         1955 c         2095 g         1298 t
ORIGIN      
        1 tccgacttct ttgcgttgcg ccgcccatcg gcatccatca actacatcgc cagccatgac
       61 ggcgcgacgc tcgcagatct cacgatgtac gagggcaagc acaacgaagc caatggcgag
      121 gacaatcgcg acggcgtgtc ggacaacctg gcgcgcaact ggggcgtgga agggccgggt
      181 gacgatgcga caatcaatgc gatgcgcgcc cggatgcgcc gctcactgct tgccacactg
      241 ttctggtcgc ggggcgtgcc catgcttctc gccggcgatg agttcggccg aacacagaac
      301 ggcaacaaca atgcttattg ccaggatgat gagatttcct ggctggattg gagcaaggct
      361 gacgagtcct tgatcgcctg gacggcacgt ctcacggcat tgcgtcgcgt gtatctcgga
      421 ttgcacgagg acaggttccg ccacggtcaa gaagtcattc ggggctttcg cgatctcgat
      481 tggctggatg aacgcggcaa aatcctctcg gaggaggatt ggcagaacgc ggaaggccgc
      541 gcgcttgcaa tgcgccggac aacgaagcgc gccgacggca aggtcgagat catcgcgctt
      601 ctgatgaaca gctccgagga tgcgctcgaa ttcacgctgc cggacggctt cgactggcgc
      661 gtcatcttcg acagcgcgga gcccgaacgt gaagcagacg cgcttcgcaa aaagtcctgc
      721 aaggtcgaaa gccatgcctg cgtgatgacc gccgcagtgg tcgaggcacc gtcatgacgc
      781 gcttccggct ctgggcgccg gcctgcacac acgtcggact ggatgtgacg ggcaatgtca
      841 tcaccatgcc ccagatcatg gacggctggt tcgatgtcga agcggatgct tctgtcggcg
      901 cgcgctatcg cttcttgatt ggagagcagg cggtgcccga tccggcgtcg cgcctgcaaa
      961 acgaaggctg gagtgtcgtc accgatccgg atgcatatcc atggacagat ggatcgtggc
     1021 gcgggcgccc gtgggaagag accatcctct acgagcttca tgtcgggttg atgggcggat
     1081 ttcagggcgt tgaagaccgg ctggctgcac tcgctgaact gggcattact gcgatcgagt
     1141 tgatgccgat cgcgcagttt ccaggaatac gcaactgggg ttatgacggt gtgctaccct
     1201 acgcgccggc ccgcgcctat ggttcaccgg acgatctgaa acgcttggtc gaccgcgccc
     1261 acgcgctcgg gctgatggtg ttcttggacg tcgtctacaa tcactttggg ccggatggaa
     1321 attatctgcc gctctatgcg ccagacttct ttcgcgagga tcgccacacg ccctggggcg
     1381 cgggcatcga cttccgcaag gaacaggtgc ggcgcttctt catcgacaat gcgctttatt
     1441 gggtgaacga gtttcacatc gacggcctaa ggctcgatgc ggtgcatgcc atcgacgaca
     1501 atgaattcct tgttgagctc gtggattcag tacgtgccgc ggcgccggag cgctacgtgc
     1561 atgtcgtggt cgagaacgag cgtaacgatg cgaagcttct ggccgagggc ggcatcgggc
     1621 aatggaacga tgatttccat cacgcggtcc acgtgctgct gaccggtgag agcgaaggct
     1681 actatagcga atatgcggac gcgcccgctc aggggctcgc gacttcgctc tccggcaaat
     1741 tcatccgcga gcgcagcagt cctggaccgg aacttgcgcc cacggcgttt gtcaattttc
     1801 tgcagaacca tgatcatgtc ggcaaccgcg ccttcggcga gcgcctgatc gcgctcgcga
     1861 atgaggacgc cttgcgcgcc gccatcgcgc tgctgctgct cgcgccgccg atcccgctga
     1921 tcttttttgg tgaggagaca ggcgcacgcg agccgttcct gtacttctgc gatcatgtgg
     1981 acgccaagct cgcggatgcg gtgcgtaaag gccggcgcaa cgagttcacc aagtttcccg
     2041 aattccagga ccccgagaag cgcgcgcaga tcccggaccc gaacgcgccc gagacgttcg
     2101 aacgctcacg gccgcacctt gatgacggcg cggagtggcg cgcgttctat aaggagctgc
     2161 tcgatctacg ccgtacgcat atcactccgc ggctcaaagg ctgcaccgcg gaaggtgctg
     2221 aagtcgtggg tccgaaagcg gtgtgcgcaa cctggcgcat gggcgatggt acgcgtctga
     2281 ccatcctgac caacctcgat cgagtgcctc cgtcgtatgt gctcccttct gcaaagccac
     2341 tctgggaccg cgccggaaca cgctgttgga tcgaaccatg atggccgaca ctgctcttta
     2401 cgaccgcgcg cgcaaggccg gcctcgcgcc ggattggatt gatgcagcgg gtgcgcacaa
     2461 gaccgtgtca ccggacacgt tgcgcgtcgt gcttgatgcc ctggacggct tcgaattcag
     2521 cgacacgcag ccggacggga gcacgacatg tttttccgtc ggcgaagcag ttcccgggcg
     2581 gcgggtggct gcgctcgccg tgcagctata tgcgctacgc ggtagcaatg gttttggcga
     2641 tcttggcgcg ctggcgcgtt ttgcgcgcga ggccgcgcct ttcggcattg atgcgttggc
     2701 cgtcagtccg atccacgcgc cgtttctcgc cgtggccgat gacatcagtc cctattcgcc
     2761 gtccagccgt ctgtttctca acccgcttta tgcggcggag gaagtacccg atgacggcgt
     2821 cgagggattg gtcgactggc cgcaggcctc acgggccaag atagagcgtc tgcgggaaaa
     2881 gttcgccaac gcgccgccgg tgcacgcgga gggacggctg cgcgatcatg cccggttcga
     2941 agcgctcgac gcacattttc gcgcgcgaaa gctgtatcgc tggcaggatt ggccgagcgc
     3001 ctatcgcaat ccgcgaggcg ccgaagtcga agcttttgcg cgcgaacatc acgccgaagt
     3061 cgattttcac atatttctgc aggcgctaac ggagctcagc ctggcaaatg cacatgctgc
     3121 ggcacgcgat gccggcatgg cgatcggctt gatcgcagat atcgccgtcg ggatgagccc
     3181 gcacggcagc cacgcatgga gcgcaccgag cgatgtgttg cgcggtctca cgatcggcgc
     3241 gccgcccgac atcttcaatc cgcagggtca gaactggggt ctcacggcgc tttctccagg
     3301 cgcgccgcgt gaagcattcg ccgatacctg ggatgctgcg atgcgttacg caggtggcgt
     3361 gcggatcgat catgcgatgg gcctcaagcg gctttgggtt attcccgatg gcgcgggccc
     3421 gcatgacggg gtctatctgc actatccgat gcataatttg ttcgcgttgc tggcggagcg
     3481 ttcgcggcac catcgcgcta tcgtgatcgg cgaagatctt ggcacggtgc cggacggctt
     3541 tcgcgatgcg atgcacagcg cgggcatgct cggcatggaa gtgttgtggt ttcagcgcga
     3601 tggaaggcgc tttctgccat ccgagcgctg gtccaaacat gccgccgcgc tcaccacgac
     3661 gcacgacctt ccgaccgtca ccggctggtg gggcgggcgc gacatcgact ggctcgagaa
     3721 gctcggtcgc aagtccgagc acggcagcat cgatgcggag cgctgggcgc gagacgagga
     3781 ccgcgcgcat ctgtggtctg tgatcggcca tgggctggcg ccttcgccgg atgaaaccga
     3841 gcgggtcctg gatgccgcga tgggctttat cggacgcacg ccgtgcgaga tcgcgatcgt
     3901 tccggttgag gacgttctgg atgttgtcga gcaacccaat atccccggca cgatcgacga
     3961 gcaccccaac tggcgcaggc gcctgccgcc gggcgattct ttggcaggtg ctgcagcacg
     4021 cgccaatctc gaggcgctcg tgcgcgagcg ttcgcggcca tgacaccgcg tgccacctac
     4081 cgcatgcagt tccacaagga cttcacgttc gcggatgcca cggcacttgc accgtatctt
     4141 tccgcgctcg gtatcagcca tctgtattct tctccgattc tggctgcgcg cgcgggctcg
     4201 atgcacggct acgatgtgat cgatcacgcc cgcatcaatc cggaactcgg cggcgaggat
     4261 ggttttcgcg cactcgctgc ggcgttgcgg gcacacgata tcggcatcgt gctcgacatc
     4321 gtcccgaacc acatggcggt cggcggtgcc gacaattcgt gttggctgga tcttttggag
     4381 aaaggccgcg acagcgtctt cgccaatatg tttgacatcg actgggaacc ggcccagcca
     4441 aatctgcgcg ataaggttct ggtgccgctt ctcggctcga aaccgcgtga cgccatcgcg
     4501 gcggacgaaa tttcactcat ttgggacgag gggctcggca agtacgcgtt cgcttatgcc
     4561 gagcaccgct ttcccttgtg cagggaagat tatgcggagg tcgaagctga tctggccgcg
     4621 gctaatgcac ccgagacgct aagcgaattg ctcgaacggc agaacttcag gctggccttc
     4681 tggcgggatg cgggtgagcg cataaactgg cggcgtttct tcgacatcac agaacttgcg
     4741 gctctccgtg tcgaagacga tggcgtgttc gagatcgttc atgccacgac cttccggctt
     4801 tacggcgaag gcctcatcga cggcgtccgt gtcgatcacg tcgacgggct cagcgaccct
     4861 aaagcctatt gccagcggct tcgcgcgcgt cttgaggcgc tcaatgcgca gcgcgagcgt
     4921 aaggaggcgc cgtatatcat cgtcgagaag attttggcgc aggacgaaag cctccccgaa
     4981 gattggggca tcgacggcac gacgggttat gacttcatgg atcagatctc ggccttgcag
     5041 catgacggtg ccaatgccga gcgattcgcg aagctgtggt cagaaatcag cggacgctcg
     5101 gcggaattcg agccggaaga acgaacggcg cggcgcgagg tgatggagag tgctttcgcc
     5161 aaccagcttc gcggggccgc catggcgttt caggcgctgg caccggatat cggccaagaa
     5221 gatttcgagg ctgcgctcgc agtcttgctg gaaaacttcc acgcctatcg cacctatacc
     5281 ggtacaccga gtccgccgtt cgacgcagcc tatcgcgcgg ctagagccgg ggcaccacgc
     5341 gctgctctca atgcgatcgc gaacgcaatc tgttccagca atgaggccgt gcgcgatgcc
     5401 gtgcgccgct tcaatcagct tgcagccccg gtggcggcga aggcggtcga ggatactgcg
     5461 ttctaccgct acggtcggct gctgtcgcgc aatgatgtcg gcttcgcgcc ggatcgcttt
     5521 gcgatttcca atgaggaatt tctgcgcaag gcggcgcacc gcgggcagct ctttcccaga
     5581 gccctgctgg cgaccgcgac ccacgaccac aagcgcggcg aggatgtgcg cgcgcggctg
     5641 gcagcgctga gcgaaattcc cgacgtctgg gaacaggaag tgcgcgcttg gtttgcagtg
     5701 aacgcgccgc taaggccttc gcaggtaacg gcgggcgacg aatatcagct ttatcaaacg
     5761 ctggtgggcg cgtggccgat tgaaaccgag cgcgtgctgg cctggcgaga gaaatcgctg
     5821 cgtgaagcga agcttcagac ctcgtggttc gcgcccaatt ccgagttcga aaaagagaat
     5881 gctgcgttcg tccaggcaat cctcgatccg agtcatgatt tcgtagggcg ccttgatcac
     5941 ttcgccgcga agctttcgcc agctggcatt tgcaacagtc tggttcaatg cgccttgcgc
     6001 tatacgctgc cgggcatccc cgatctgtat caagggagtg agcttgggga tttcagcctt
     6061 gtcgatccag acaaccgccg tcctgtagat tatactttgc ggcaagaatt gctttccgct
     6121 ccggtctcgc tggacgggtg gcaaatgggt acgccaaagc tgggcttgat ccggcgcctg
     6181 ctggcattgc gcagagagcg gcctgaagtg ttcgatgcgg atatggagtg tgtgacagcc
     6241 tccaggcatt tgttggtgtt tcgacggcag tcgcttcttg tcgccgtacc actcctttcg
     6301 tcctattcct gcatggagcg cggcgttccg ctcccgatag tcacgggcac acttcacccc
     6361 gaagggcagt ggcgcaacgc gcttgacccg tcgctctcgc gggtgcgttc gctcgattgc
     6421 gccgagctgt tcagcacgtt tcccgtcgcg gtattggtgt cgtgacggtg cgcatcggca
     6481 tatcgggatg gcgctatgcg cgttggcgcg gcgtgttcta tccggaaa
//
LOCUS       DASZMC010000294         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4255423,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000294 DASZMC010000000
VERSION     DASZMC010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4255423"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VG274_06025"
     CDS             <1..298
                     /locus_tag="VG274_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06246.1"
                     /translation="RRSWFRTSAFALSARAPIESLSTSYDDSDIDPFKGGVKAMPKKK
                     TPARIPWTKDDIRQLKAHSKTRTPVVEVAKAMKRTEAAVRQKAKTIGIGLGHRR"
     gene            1144..1515
                     /locus_tag="VG274_06030"
     CDS             1144..1515
                     /locus_tag="VG274_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06247.1"
                     /translation="MSKSVLSSLSVAALAGGAMLALELSPASAFTLSGPSAQLVTSGQ
                     FDKVYYRGYHGGYHGYHGGYHGGYAYRGGYGYHGGYGYRPYGYGGAAAVGAAAIGAAA
                     IGAAVAAPHCWINRYGTQVCN"
     gene            complement(1577..1790)
                     /locus_tag="VG274_06035"
                     /pseudo
     CDS             complement(1577..1790)
                     /locus_tag="VG274_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          379 a          572 c          610 g          352 t
ORIGIN      
        1 gaggcggtca tggtttcgaa caagcgcgtt tgcgttgtcg gcgcgggccc caatcgaatc
       61 gctcagcacg agctacgatg atagcgacat cgatcccttc aagggaggag tgaaagccat
      121 gccgaaaaag aagactcccg ctcgcattcc gtggactaaa gacgacatac ggcaactcaa
      181 agcccattcg aaaacgagga cacctgtggt ggaggtcgcg aaggcaatga agcgaaccga
      241 ggcggcggtc cggcaaaagg cgaagacaat cggcatcggt cttggtcatc gccgttgagg
      301 acgctcactt ttgatcgact ctcccgaggg caacctaacg cgaagtttgg atggccacct
      361 gcgctccgca atgcaaggaa ccccacctca gagcatgtcg gccgcggctg gagaacagac
      421 gatcgacatc acatcttcca gtgaaccgcc gcggtcctgt gcggtgctca cgcgcgatcg
      481 tcgattaagc atccgacgcg cctaagcgaa gcgcaggagg aagcgccaca gcccgtatgg
      541 caatatcaga gtgatcaccc ggaccaaaag ctttcaccga aggcggacgc ggttccctcc
      601 gaaagttcgg gccgagaccg gcgtagtcgc cccggcccga cttattttgc gccaccaaaa
      661 cagcggagaa taggcgggat gtgggcgagc ggacccgaag gcccccggcc taaaattcta
      721 ggccatattg gccggggggc gcgtcgcatg acgcggggag gagtcgcgtc tcggtccgtc
      781 gcagcgtgca ggctgtccgc ggcttcgtga ttcgtcaacg accccgcgtt ggctcaagag
      841 ccgttatccc cagccctcgc ttatcggctc gccgctgccc gaagacgcgc ctctagggcc
      901 gtccatcgtc aggttggcca attgccttat tcatgggttc gaccgacgca cgtgacgtcc
      961 gcgaccactc gccccgcgaa cctcgcgccc tgtccacgtc gagcgcgcgc atttgacggc
     1021 gcgcgtgggc tgcccgtgtc cgaaacaagc cagtggcgca aacgaaagcg cgggctgtgt
     1081 tcagatggcg ttcacttcca agggcgtcta accgtgagcg tggggagcgc aacggaggaa
     1141 gtcatgtcga agtcagttct ttctagcctg tcggttgccg ccctcgctgg cggcgcaatg
     1201 ctcgcgctcg aattgagccc cgcatcggct tttacgcttt ccggaccatc cgcgcagctc
     1261 gtcacttcag ggcagttcga caaggtctac tatcgcggct accacggcgg ttatcacggc
     1321 taccacggcg gttatcacgg cggctacgca tatcgcggtg gttacggtta ccacggcggc
     1381 tatggctacc gtccctatgg atatggcggc gcggcggcgg tcggcgcggc ggccatcggc
     1441 gcagcggcga taggcgcagc ggtcgcagca ccccattgtt ggatcaatcg ctacggcacc
     1501 caagtctgta attagtctga cgggcgttga cacgtgatcg aagcgccgac gcgaagcggg
     1561 attgcagtgc ggaacttcaa gcggccgcgg cgacctcgcg ccacgtggtc ctcgcagcgg
     1621 cgcgtagcac gcggtgcgat tggctgatat gagatggcgt tggacgttga agatgttgta
     1681 aacggcggcg tgagttgaga ggaatttgtg tgtagagcga ggctcttgaa acgttgcatc
     1741 ttgcgctccc gccgtcgggt cggctaatgc gaattttcgg cccgattgtt ctgtgtgcct
     1801 tggcaaggcg catcgttctc cccggtggaa atccgggccg gcaactctcg ctccagccgg
     1861 aagcgatcgg agcggatcag gaggcgacga atgatctgaa gcacttcgat aga
//
LOCUS       DASZMC010000295         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5062866,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000295 DASZMC010000000
VERSION     DASZMC010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5062866"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VG274_06040"
     CDS             complement(<1..1212)
                     /locus_tag="VG274_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWE06248.1"
                     /translation="MNFDSTSDAAELFAAQERREPKKQRIIGHLVSLTGMHGLIACEM
                     TPEEAGDHWSVGSLISVVHANSRLVGVVCELVTSDSRWRESESNLALIKIELSGEIID
                     EAPGKPVFHRGIRSFPTLGSMAHGIRASDLRAIYSIRGGLGVEIGRLTQNETIPATIN
                     VRELVTRHFAVVGSTGVGKTTAVSMLLKTCLRERPNLRVMIIDPHNEYAAHFIRDSVV
                     LDSDNLELPYWMFRFEEMLDIIYAGRKPNPDESDALYEVIKSAKTRFSAASAATRLSA
                     VRRQPNAESGWVSADTPVPYRISDAVQVIEEWMGKLESRYARADMRALKHRLEALSHD
                     PRFRFMFGKLMVEDIMSKVVSQIFRMPKGGAPVTIVKLAGLPNEVVNSVVSVLARMAF
                     EIALWSDANYEI"
BASE COUNT          270 a          466 c          476 g          288 t
ORIGIN      
        1 gatctcgtaa ttcgcgtcgc tccagagcgc gatttcgaac gccattcgcg ccagcaccga
       61 aacgacggaa ttgacgacct cgttcggcag gcccgcgagt ttcacgatcg tcaccggcgc
      121 cccgcccttc ggcatgcgga agatctgcga cacgaccttg gacatgatgt cttcgaccat
      181 gagcttgccg aacatgaagc gaaagcgcgg atcgtggctc aacgcctcca accgatgctt
      241 gagcgcgcgc atgtcggcgc gggcgtaacg ggattcgagc ttgcccatcc attcctcgat
      301 gacctggacc gcatcggaaa tgcgataggg cactggggtg tccgcgctga cccagccgct
      361 ctccgcattc ggctggcggc gaaccgcgct caggcgcgtg gcggcggaag ccgcgctgaa
      421 ccgggtcttg gcggacttga tgacctcgta gagcgcgtcg ctttcgtcgg gattgggttt
      481 gcggccggcg tagatgatgt ccaacatctc ttcgaacctg aacatccaat agggcagttc
      541 tagattgtcc gaatccagca cgaccgaatc tcggatgaaa tgcgcggcat attcgttgtg
      601 gggatcgatg atcatgacgc gcagattggg gcgttcgcgc aggcacgtct tcagcagcat
      661 cgaaaccgct gtcgtcttgc cgacgccggt ggacccgacc accgcgaaat ggcgggtgac
      721 cagttccctg acgtttatgg tcgccggtat cgtttcgttc tgagtgagcc ggccgatttc
      781 cacgccgagg cccccacgta tggagtagat cgcccgcagg tcggaggcgc ggatgccatg
      841 cgccatcgaa ccgagcgtgg gaaaggaacg aatgcctcgg tggaatacgg gtttccccgg
      901 cgcctcgtcg atgatttcgc cgctgagttc gatcttgatc agcgcgagat tgctttcgct
      961 ctcgcgccat cggctgtcgg acgtcacgag ttcgcagacg acgcccacga gccgcgagtt
     1021 ggcgtgcacc acggaaatca aactgccgac ggaccaatgg tctccggctt cttccggggt
     1081 catttcgcag gcgatcagac catgcatgcc ggtcagcgaa acgagatggc cgatgatgcg
     1141 ctgcttcttg ggctctcggc gctcctgagc cgcgaaaagt tcggccgcgt ccgatgtcga
     1201 atcgaagttc atgggttttc ctcccgtggc ggctccgccg ggggcccggc ccgcccaatc
     1261 gctcgccgac ctcggggtcg acgccgtcat agtccggcca tcgtggggga aaacagtaaa
     1321 cattgcctat cacgaatcga cgcgaatctc ggggtcgcct ccgaccagtc gcgagaactg
     1381 cttgaggcga ctctgcaggc ggtcgctgtt gagcggcgcc aggtcggagg gcagcgtcaa
     1441 gaccacctta cccttctgcg cggtcatcgg ggcccgcggc agcacgccgg gcatggcggc
//
LOCUS       DASZMC010000296         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_117433,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000296 DASZMC010000000
VERSION     DASZMC010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117433"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..413)
                     /locus_tag="VG274_06045"
     CDS             complement(150..413)
                     /locus_tag="VG274_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HWE06249.1"
                     /translation="MHKFELTLQGYRLTTAEILYHMPDHPAVLQSYIWQELDIAPKFP
                     ILTRFLCFWRDNLEGKLHSVRVAARGLISPAELKHAGSEFTLH"
     gene            643..1632
                     /locus_tag="VG274_06050"
     CDS             643..1632
                     /locus_tag="VG274_06050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HWE06250.1"
                     /translation="MTDSFRALLISKDGEGQKVDEAELSTGDLMDGNVVIAVSFSTMN
                     YKDALALTGRAPVVRRFPMIPGIDLAGTVDTSEDSAFRPGDRVLINGFGLGETHYGGY
                     AKRARVKSEWLLPIPGVFSFAEAMAIGTAGYTAMLAVLALEDAGVKPENGPAVVTGAS
                     GGVGSVAVALLSKLGWRVIASTGRVAEEAYLKNLGAQELIARTELAGEPRKLAKERWA
                     AAIDGVGSKTLANVIAATSYGGAVAACGLAGGMDLPTSVAPFILRGVSLLGIESVNMK
                     MPRRRQAWERLARDLDREKLAAMTRTIELHEVPRAAEDVLEGRIRGRLVVRVA"
     gene            1664..2323
                     /locus_tag="VG274_06055"
     CDS             1664..2323
                     /locus_tag="VG274_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HWE06251.1"
                     /translation="MNYIVLIVTGLIIGFIVAAPIGPVNLICIRRTLAFGSLNGFLSG
                     LGGAMGDGVYAAIVGFGLTAISQWIEGYSQALQMAGGALLLGYGVHTYLADPLRGKQV
                     GYPADRSLGNSSLARAFASTFAISITNPATLFAFAALFTGFGGILAGARASLAQAGFV
                     VGGVLCGSALWWLTLTTVVGFLHARIDARVMRIITHVTGVIVIVFGIGMLGHVLLGRL
                     R"
     regulatory      2479..2570
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2732..3874
                     /gene="gcvT"
                     /locus_tag="VG274_06060"
     CDS             2732..3874
                     /gene="gcvT"
                     /locus_tag="VG274_06060"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF010093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWE06252.1"
                     /translation="MSDSTDQTLRRTPLFALHIELGGKMVPFAGYALPVHYQAGILKE
                     HLHTRAQAGLFDVSHMGQAALSGHEPGAALESLTPADVKGVKEGQQRYALLLNAAGGI
                     KDDFMVARLGGESALHLVVNGATKEGDFTYIAEQLKGRATLTPHPDRALLALQGPASE
                     MVLSRHAPAAKNLTFMKVARMDVAGAPAIVSRSGYTGEDGFEISIDAGDAERVARALL
                     NEPDVLPIGLGARDSLRLEAGLCLYGHDLNEDIDPVSANLVWSIGKRRKADRDFPAAG
                     QILKLLSDGPREKRVGIRPEGRAPAREDAPIADRTGRIIGRVTSGGFGPSANAPVAMG
                     YVETAFAADGTEIDLLVRGKPLAARVAPTPFVPHRYRRAETVSKAG"
     gene            3880..4254
                     /gene="gcvH"
                     /locus_tag="VG274_06065"
     CDS             3880..4254
                     /gene="gcvH"
                     /locus_tag="VG274_06065"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWE06253.1"
                     /translation="MSEVRYTDQHEWVRIDGDIATVGITKYAAGQLGDVVYVELPEAG
                     RKVGQGGEIAVVESVKAASEVYAPVGGEISEANAALAAEPAKINADPEGEGWFFRMRL
                     ADKSELGKLMSEAQYTEFVKLL"
BASE COUNT          783 a         1262 c         1431 g          804 t
ORIGIN      
        1 tttagcgcag aggacgcaga ggtttcgcgg agggcgcaga gaaatgtggc gggctttgcg
       61 cgctatatgt tctccgcgtc cactgcgctg cctccgcgct cctctgcgtt aaatcttcct
      121 tgcgcatcgt caagctccga attgttgtac taatgcagcg tgaattcgga gccggcgtgc
      181 ttcagttccg caggcgagat gaggccgcgc gcggcaacgc ggaccgaatg gagcttgcct
      241 tcgaggttgt cgcgccagaa gcagagaaag cgggtgagga tgggaaattt cggcgcgata
      301 tccagctcct gccagatgta gctttggagc accgccggat gatcgggcat gtgataaaga
      361 atttcggcgg tcgtcagccg atagccctgc agcgtcaatt caaacttgtg catttgtgaa
      421 tcgcctttgc gtcttcggtt tcctgggcct ttctccctgc caatgaaggc acagattcgt
      481 aaggaaaaag tttgggcagc ggttccctcc ctgtccagtc cgccgggaga cgaaccccag
      541 tgaaagggcc ggcttcttga cggcaggcgt tatttttgtg ccttagtgtt tcgcgcgcga
      601 cacgcctgcc gttctggcag cagtcacttt ccggaagtgc caatgacaga cagcttcagg
      661 gctctcctga tttcgaaaga cggcgaaggc cagaaggtcg acgaggctga actttcgacc
      721 ggcgatctca tggacggcaa cgtcgtgatc gccgtttctt tctcgacgat gaactacaag
      781 gacgctctcg cgctgaccgg acgcgcgccg gttgtgcgca gatttccgat gatccccgga
      841 atcgatctcg cgggcacggt cgacacgtcc gaagattccg cattccgtcc cggcgacagg
      901 gtgctgatca acggcttcgg actgggcgaa acgcactatg gcggctatgc aaaacgggca
      961 cgcgtgaaaa gcgaatggct gttgccgatt ccgggagtgt tttcgttcgc cgaggcaatg
     1021 gcgatcggca cggcgggcta caccgcgatg cttgccgttc ttgcgcttga ggacgcaggc
     1081 gtgaagccgg aaaacggacc cgccgtggtg acgggtgcgt caggcggcgt cggttcggtt
     1141 gcggtcgcgc tgctgtcgaa gctcggctgg cgcgtgatcg cttcgaccgg ccgcgtcgcg
     1201 gaagaggcat acctgaaaaa cctgggcgcg caggaactca ttgcgcgcac agaacttgcc
     1261 ggcgagccgc gcaagctcgc gaaggaacgt tgggcggccg ccatcgacgg cgtgggttcg
     1321 aagacactgg ccaacgtcat cgccgccacg agttatggcg gagccgtggc ggcgtgcggt
     1381 cttgccggag ggatggattt gcctaccagc gttgcgcctt tcattttgcg gggagtctcg
     1441 ctgctcggaa tcgagtcggt caacatgaaa atgccgcgaa gacggcaggc gtgggagcgc
     1501 cttgcgcgcg atctcgatcg ggagaaactc gctgccatga cgcgcacgat cgaactgcac
     1561 gaagtcccgc gagctgcgga agacgttctc gagggccgga tacgcggtcg gctcgtggtc
     1621 agggtcgcgt gatgaagaat cgtccgcagg ctgcccagcg cgcatgaact atattgtgct
     1681 catcgtcacc ggtctcatca tcggtttcat cgtcgcggca cccatcgggc cggtcaatct
     1741 gatctgcatc cgccgtacgc tcgcattcgg atccttgaac ggcttcctgt ccgggctggg
     1801 cggcgcgatg ggcgacggcg tctatgcggc gatcgtcgga ttcggtttga ccgccatcag
     1861 ccagtggatc gaaggctatt cccaggcgct gcagatggcg ggcggcgcgc tccttctggg
     1921 atatggcgtc cacacctatc tcgccgatcc attgcgcgga aaacaggtcg gatatcccgc
     1981 ggatcggtcg ctcggcaatt cctcgctcgc gcgcgccttc gcttcgacct ttgccatctc
     2041 gatcaccaat ccggcgacgc tgttcgcgtt cgccgcgctg ttcaccggct tcggcggtat
     2101 cctggcgggc gcgagagcca gtcttgcaca ggccggattt gtcgttggcg gcgtgttgtg
     2161 cggctcggcc ctgtggtggc tgacgctcac cacggtggtc ggctttttac acgcccgcat
     2221 cgatgcgcgc gtgatgcgga tcatcaccca tgtgacgggc gttatcgtga tcgttttcgg
     2281 aataggcatg cttggtcatg tgctgctcgg acggctgcgg tgagcatggc aggtcggcgt
     2341 gaccagcgac ctcgttcggc gcgcctcatg cgaacggcga actgccgaga gaggcggttc
     2401 cgcgcgttga cttgaacgcg ttcccattta tgatgccgac aacgtcatgg tccgccgtga
     2461 ggcgcgctgc tgacggcgac atcccccgcg ggagagattc gcccgaatag gcgaacgccg
     2521 aaggagcaac cgccccggaa actctcaggc aaaaggaccg cgtggggaaa tgaaactctg
     2581 gaaagcagga tggtgacgac gccatcctca ccgaaggggt aagccgggcg aggtgaaatc
     2641 tctcgcaggc gaaatctctc aggtccgatg acagaggggc gcgggttccg gtccgccggg
     2701 cgcgccgttc caacctcttt gccggaccgc gatgtcagac tcgaccgacc agactctccg
     2761 ccgaacgccg ctcttcgcgc ttcacatcga gctgggcggt aaaatggtgc ccttcgcggg
     2821 ttacgcgctg ccggttcact accaggcggg cattctgaaa gaacatctgc atacgcgcgc
     2881 gcaggccggg ctgttcgacg tctcgcatat ggggcaggcg gcgctttccg gtcatgagcc
     2941 aggggcggcg ctggaatcgc tgacgcccgc cgatgtgaag ggcgtcaagg agggccagca
     3001 gcgttacgcg ctcttgctca atgcggcagg tggcatcaag gatgatttca tggtggcgcg
     3061 gcttggagga gaatccgcgc tgcacctggt ggtgaacggc gcgaccaagg aaggcgattt
     3121 cacctatatc gccgaacagt tgaaaggccg cgccacgctc acgccgcatc cggaccgcgc
     3181 actgctcgcg ctgcaagggc ctgcctcgga gatggtgctt tcgcgccacg caccggccgc
     3241 gaagaatttg accttcatga aggtcgcgcg gatggacgtc gccggcgcgc ccgcgatcgt
     3301 cagccgctcg ggctataccg gagaggacgg ttttgagatc tccatcgacg ccggcgatgc
     3361 ggagcgtgtc gcgcgtgcgc tgttgaacga gcccgacgtg ctgccgatcg gacttggggc
     3421 ccgcgactcg ctgcggctcg aagcgggtct ctgcctctat ggacacgact tgaacgaaga
     3481 cattgacccc gtcagcgcca atctcgtctg gtccatcggc aagcggcgga aggccgaccg
     3541 ggattttccc gctgccgggc aaattctgaa attgctttca gacggcccgc gcgagaagcg
     3601 cgtcggcatt cgtcccgagg gcagggcgcc tgcacgcgag gacgcaccga ttgcggacag
     3661 gacgggccgc attatcggac gggtgacgag cggcggtttc ggcccgagcg ccaatgcgcc
     3721 ggtggcgatg ggttacgtcg agacggcgtt cgcggcggac ggcacggaga tcgacctcct
     3781 cgtgcgcggg aagccgctcg cggcgcgcgt ggcgccgacg ccgttcgtgc cgcatcgcta
     3841 caggcgcgcg gaaactgttt caaaagcggg gtgagaagaa tgagcgaagt acgctacacg
     3901 gatcagcacg aatgggtgcg catcgacggc gacatcgcaa ctgtcggcat caccaaatat
     3961 gcggcgggtc agctcggcga cgtcgtctat gtggaattgc ccgaagccgg gcgcaaggtc
     4021 ggacagggcg gcgagattgc cgtcgttgag agcgtgaaag ccgccagcga ggtttacgcg
     4081 ccagtcggag gggaaatcag cgaagcgaac gcggcgctcg ccgccgaacc ggcgaagatc
     4141 aatgccgacc cggagggcga aggctggttc ttccgcatgc gcctggccga caaatccgaa
     4201 ctcggcaagc tgatgagcga agcccagtac acggaattcg tcaaactact ctgatcaagg
     4261 tgatgcgtgc agcgaaaaag
//
LOCUS       DASZMC010000297         4020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5065907,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000297 DASZMC010000000
VERSION     DASZMC010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5065907"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VG274_06070"
     CDS             <1..682
                     /locus_tag="VG274_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06254.1"
                     /translation="TPDDAARLLKRVAEAGANPALTFHVGSQCLSPFSYAQAIEMARR
                     TAALAGIDIAALDIGGGFPWSYPGHDVPPYHWYFDTIREALATLPDADRLPLLCEPGR
                     GLVAEGMSLVTRVVLRKGDKLYINDGIYGSFDELTLPGWTTDYHLRVFALDEQGGVSA
                     KADRSSAFRVYGPTCDTLDVLPRPLMLPETIAPGDFIVFDEIGAYSCAVRTDFNGFLP
                     DRWAIVGN"
     gene            787..1188
                     /locus_tag="VG274_06075"
     CDS             787..1188
                     /locus_tag="VG274_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWE06255.1"
                     /translation="MGAGMSSSHNENSIGTAIVAGAMTVHSALGPGLLESAYEFCLAH
                     ELSKRGLDIRKQVSIPIVYDELSIENGYRIDLLANDLVVVELKAIEAVTPVHRAQLLS
                     YLRLGGFKLGYLLNFNVAHMRDGVMRLVNGL"
     gene            complement(1276..1704)
                     /locus_tag="VG274_06080"
     CDS             complement(1276..1704)
                     /locus_tag="VG274_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HWE06256.1"
                     /translation="MNRRESMVRLKRFRVDELKRHMATLDGMRADVARKLTDLEDSIA
                     RERQRANDSDLGRLAFPSFLKAMDARRDNLQATLREIERERGQAQNVFGDAHQELKSL
                     ELANEQDVKRAAESQLRKSGARLGDLALSRQLRKQALRHV"
     gene            2073..2765
                     /locus_tag="VG274_06085"
     CDS             2073..2765
                     /locus_tag="VG274_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWE06257.1"
                     /translation="MRVLLIEDDSAMAKSIELMLRSEGFNVYTTDLGEEGIDLGKLYD
                     YDIIVLDLQLPDMSGFEVLKSLRLAKVQTPVLILSGNAIVEAKVKALGFGADDYMTKP
                     FHKDELVARIQAVVRRSKGHSQSVITTGKLIVNLDAKTVEVDSSRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAAATDGENYIETVWGRG
                     YVLRDPVAEVAA"
     gene            2960..3838
                     /locus_tag="VG274_06090"
     CDS             2960..3838
                     /locus_tag="VG274_06090"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HWE06258.1"
                     /translation="MMDVLHSIDVAGWSGPFAADTQEGACDALESGRLLFFPHLEFRL
                     AEHERLLLTPDVSDGKSKNVSLKGSGELGGTSCTGEQASLLQQMMERFARDAARLVEA
                     LIPAYANRLARAPTSYRPVEIAGRSASAIHDDTRLHIDAFPSRPMRGKRILRLFSNIH
                     PGGAPRVWHVGEPFEAMARRFLPAAREGSPAQAWMMNLLGVTKGRRSAYDWLMLGLHD
                     GAKLDAEYQQRCAQTEIGFRAGSTWICFTDQVMHAALAGQYVLEQTFHIEVDAMVAPD
                     RAPLRVLERLRGHALV"
BASE COUNT          745 a         1293 c         1224 g          758 t
ORIGIN      
        1 cacgcccgat gacgcagcgc gcctgctgaa acgcgtcgcc gaggcgggcg caaatccggc
       61 gctcactttc catgtcggct cgcaatgcct gagcccgttc tcctatgcgc aggcgatcga
      121 gatggcgcgg cgcacagccg cgctcgccgg catcgacatc gccgcgctcg acataggcgg
      181 cggatttccg tggtcgtatc cggggcacga cgtgccgccc tatcattggt atttcgacac
      241 gatccgcgaa gccctggcga cgctgcccga cgccgaccgg ctgccgcttc tgtgcgagcc
      301 gggacgcgga ctcgtcgccg aaggcatgtc gctcgtcacc cgggtcgtgc tgcgcaaggg
      361 cgacaagctc tacatcaatg acggcatcta tggcagcttc gacgaactga ccctgcccgg
      421 ctggacgacg gattatcatc tgcgcgtttt cgcgctggac gaacagggcg gcgtcagcgc
      481 caaagccgac cgttcgagcg cgtttcgcgt ctacggcccg acctgcgaca cgctcgacgt
      541 gctgccgcgc ccgctcatgc tgcccgaaac gatcgcaccg ggcgatttca tcgtcttcga
      601 cgagatcggc gcctattcct gcgccgtgcg caccgacttc aacggcttcc tccccgaccg
      661 ctgggccatc gtgggcaact aaccgtcggc cacggtcgca ttgcgtcgca attccaaact
      721 tcgctacaaa acggacaagc aaagcaggaa gaaagaattt aacgcggtgg gcgcagagga
      781 tgcgcggtgg gcgccgggat gtcgagcagc cacaatgaaa acagcatcgg caccgcgatc
      841 gttgcaggcg ccatgacggt tcactcggcg ttggggcctg gcctgctgga gagcgcctac
      901 gaattctgtc ttgcgcacga actctcaaaa cgcggactcg acatccgtaa acaggtatca
      961 attccaattg tttatgacga actgtctatt gaaaacggct accgcatcga cttgcttgcg
     1021 aacgatctcg ttgtggtcga gttgaaggcg atcgaagcag taacgcctgt tcatcgcgca
     1081 caactcttga gctacctgcg cctcggtgga ttcaagctcg gttatctgtt gaacttcaac
     1141 gtggcgcaca tgcgtgacgg cgtaatgcgc ctggtcaacg gcctttgagg tttcggcgcg
     1201 cgacgcgcgc caatccttcc cctgcgtact ctgcgcatcc tccgcgtcca ccgcgttaaa
     1261 atctttctta cgcagtcaga cgtggcggag ggcctgtttg cggagctggc ggctcagggc
     1321 gaggtcgccg agccgggcgc cgcttttgcg cagctggctt tccgcggcgc gcttgacgtc
     1381 ctgctcgttg gcgagctcga ggcttttcag ttcctggtgg gcatcgccga acacgttctg
     1441 ggcctggccg cgctcgcgct cgatctccct gagcgtcgcc tgcagattgt cccgccgcgc
     1501 atccatcgcc ttgaggaagc tcgggaaggc cagccgcccc agatccgaat cattggcccg
     1561 ctggcgctcg cgcgcgatcg agtcttcgag gtcggtgagc tttcgggcca cgtcggcccg
     1621 catcccgtcc agcgtcgcca tgtggcgttt cagctcgtcc acgcgaaagc gcttcagccg
     1681 gaccatgctt tcccggcggt tcatgccggc ctgccgcggc cgggccgctt cgggatccgc
     1741 gccggcccgg ggcgcttcag gccgtcccgt gcgcccccgg agggcccggg cgccgaaacc
     1801 tcgatatccg tcggcaaagc cctcatcgac agcctctcac gcagaattcg gccccagcga
     1861 ccacaatcat cttgaacgga agggtttaaa tggcggtaaa cttaacgaag cgaatcaagg
     1921 ggatgcgcag ggtgaccgac tcatgttccg ttaatccgcc ttggctcgtg atgaagcgcg
     1981 tggacggcca acaagccgtt gttaaccatt tccggttacc ttccatgccg tcgagttaac
     2041 cggcctgagg ctcattaggg ggcgtgacca gaatgcgtgt gcttttgatc gaagacgaca
     2101 gcgcgatggc gaaaagcatc gaactgatgc tgcgctcgga gggtttcaac gtctacacca
     2161 ccgacctcgg cgaagagggc atcgatctcg gcaagctcta cgattacgac atcatcgtcc
     2221 tcgatctgca gctgcccgac atgagcggct tcgaagtgct gaaatcgctc cggctcgcca
     2281 aggtgcagac ccccgtcctc atcctgtccg gcaacgcgat cgtcgaggcc aaggtgaagg
     2341 cgctgggctt cggcgccgac gactacatga ccaagccctt ccacaaggac gaactggtcg
     2401 cgcgcatcca ggcggtggtg cgccgctcca agggccattc gcaatcggtg atcaccaccg
     2461 gcaagctgat cgtgaatctc gacgccaaaa cggtggaagt cgacagcagc cgggttcacc
     2521 tgaccggcaa ggaataccag atgctggagc ttctctcgct ccgcaagggc accacgctca
     2581 ccaaggaaat gttcctcaat catctctatg gcggaatgga cgagcccgaa ctgaagatta
     2641 tcgacgtctt catctgcaag ctcagaaaga agctcgcagc ggcgaccgac ggcgaaaatt
     2701 acatcgaaac cgtctggggc cgcggctacg tgctgcgcga tccggtggcc gaagtcgcgg
     2761 cctagactcc agaaggaaga ttttaacgca gaggacgcag aggatgcgcg gtggacgcag
     2821 aagatttttt gtgcgcttcg cgcgcgatat tcccgcgtcc cctgcgcacc ctctgcgtcc
     2881 cctgcgttaa atcttttttc tctcacgact gatcttgggg cggctttcgg tttgcggcgc
     2941 gcgcgcctat tctcgaagca tgatggacgt tctccattcg atcgatgtcg ccggctggag
     3001 cggaccgttt gccgccgaca cgcaagaagg cgcatgcgac gcgcttgaaa gcggcaggct
     3061 gctgttcttt ccgcacctcg aattccggct cgctgaacac gaacgcctgc tgctcacccc
     3121 ggacgtctcc gacggaaagt ccaagaacgt cagcctgaaa gggtcgggcg aactcggcgg
     3181 cacatcctgc acgggcgaac aggcgagcct gctgcaacag atgatggaac gtttcgcgcg
     3241 ggatgccgcg cgcctggtgg aagcgctgat ccccgcctat gcaaatcgcc tcgcccgcgc
     3301 gccgaccagc taccggccgg tggaaatcgc cgggcggtcg gcatcggcga tccacgacga
     3361 cacgcggctg cacatcgatg cgtttccgtc gcggccgatg cgcggcaaac gcatcctgcg
     3421 gctcttctcc aacatccatc cgggcggcgc gccgcgcgtc tggcatgtgg gcgagccgtt
     3481 cgaggcgatg gcgcggcggt tccttcctgc ggcgcgcgaa ggctcgcccg cgcaagcctg
     3541 gatgatgaac ctgctgggcg tcaccaaagg aaggcgatcg gcctatgact ggctgatgct
     3601 cggtctgcat gacggcgcca agctcgacgc agaataccag cagcgttgcg cgcagacgga
     3661 aattggattt cgcgcgggct cgacctggat ctgcttcacc gaccaggtga tgcatgccgc
     3721 gctcgccgga caatacgtgc tcgaacagac attccatatc gaagtcgatg caatggtggc
     3781 tccggatcgc gcgcccttgc gcgtactcga acgcctgcgc ggccacgcgc tggtgtgaac
     3841 cgcgcggcac attgagaaca aattattccg gcggggtgcc cctcacccac gctgcggcgg
     3901 ttgatggtcc tcgtgcttag cccctctccc acaaggggag aggggaatcc cagcgctgat
     3961 ttaccgatgc tgatgcaaga ttccgattct cctctcccct tgtgggagag ggatgcagat
//
LOCUS       DASZMC010000298         8727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3145463,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000298 DASZMC010000000
VERSION     DASZMC010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3145463"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VG274_06095"
     CDS             <1..601
                     /locus_tag="VG274_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWE06259.1"
                     /translation="ADLLDMIPFLRAFSRSLCGNQEMADDLAQETLVKAWQARNMFAP
                     GTNLKAWLFTILRNQFYSDRRRAWRQAPWDQDAAERIPGSSAEQASAAELSDTARALS
                     RLSDEQREALILVGAGGFSYEDAAAICRCAVGTVKSRVARARKSLLSILEGEDPLPPA
                     SHGAEGDAANEIMAELDRLSPGASQGVVARRRTKGATSA"
     gene            510..2312
                     /locus_tag="VG274_06100"
     CDS             510..2312
                     /locus_tag="VG274_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWE06260.1"
                     /translation="MRLWRNWIASRPERRRVWWHDGGPREPHRPSGWSLRLRLLVIVT
                     IALLPIAFAGFVQGLDRARRDVAQIRERLVADARLSAAPEQNVLAAAEHIGRVLASLP
                     QVSNATDSCEDELASALKGISFISNASRIDRRGRVACSAHPAAGGLPAGNLSNWRALR
                     SDADFQISGQSHSTVTGEPIVLVRLPLRDAAGQFDGALDMEIEARWFDDMIRRSRLPR
                     GSVVALFDRSGAIIASNDPARAIRLFPRGSLIGNAETVHVASDRQGKNWIYAAAPLLA
                     ENVFVGFAVPEAGALRPTHIRVATDFSLPFAMLALSWLAIWIATERQVTRWIVYLRRI
                     ATAYRAGHYALRPKLAGASREFRVLGDSLSTMADSIQERDRSLREALAQKSLLIKEVH
                     HRVKNNMQIVMSLLSLQAGRLRDPAAQDALRQARGRINALALVHRILYEIEDQQSVDI
                     KPLLEQLADQTREGFGGERREIRSVVEAESLELPAESAVPVALFVVEALTNAFKHAFP
                     LGRGGTVALCLHRLDSDTLKLAVKDDGIGLDESRLETSIGGRLISTFGQQLGGRAQIH
                     SSGSGGTAVELIFPQPVATGEYPRPALPKADAAE"
     gene            complement(2319..2624)
                     /locus_tag="VG274_06105"
     CDS             complement(2319..2624)
                     /locus_tag="VG274_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06261.1"
                     /translation="MPRGDKSSYTAKQQRMASHIEEGYEERGIGEDEAERRAWSTVNK
                     ETGGGKKSASGRGKRVNRAPSRKGGKLGGRASAKRPAAARSASARKAAATRKRRRKS"
     gene            complement(2745..4127)
                     /locus_tag="VG274_06110"
     CDS             complement(2745..4127)
                     /locus_tag="VG274_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE06262.1"
                     /translation="MTHSPDGLPLPQRNWAIATVALGITMSVLDSAIANVALPTIARD
                     LHTDAAFSIWVVNGYQLAITISLLPLSSLGDIVGYRRIYIAGLALFTAASLACALSQT
                     LTELALARVLQGFGAAGIMSVNTALIRFIYPQSQLGRGIGINTVVVAISAAAGPTIAS
                     GILSVASWPWLFAVNVPIGILAVAIAARALPKTPLGKHGFDFAGALLSAGTFGFLIGG
                     IDAGGHGEAGKLVAVELTLAIICGYFLVRRELARPAPLFPVDLLGIPIFALSVGTSIC
                     SFAAQMLAFVALPFHLQQEFGYSAVQTGLLITPWPLAIAFAAPVAGRLADRHPAGLLG
                     AIGLLIFACGLVALALLPQRPQLVDVAWRMALCGLGFGFFQSPNNRALIGSAPRHRSG
                     GASGMLGTARLLGQTMGAALVALLFGQIAAHATAYALYIAAGFALAAAAVSSLRLLQS
                     APEALPPPLD"
     gene            complement(4337..5752)
                     /gene="lpdA"
                     /locus_tag="VG274_06115"
     CDS             complement(4337..5752)
                     /gene="lpdA"
                     /locus_tag="VG274_06115"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWE06263.1"
                     /translation="MAETIKTKVLVIGGGPGGYVAAIRAGQLGLDTILVEADRVGGTC
                     LIRGCIPSKAMIHVAGEFEAIARASGKKGRHGISLASPPNLDMRELVRWKEGIVTKLS
                     MGVATLCKRAKVRVIAGWARFTDAKTCMVEDGTNSVTITAEHVILANGSVATELPFLP
                     FAGPVISSSEALSLDALPQKLVVVGAGYIGLELGIAFRKLGSQVTVVEALDRILPLFD
                     AELAAPVNRWLQRAGVAVHLGAQAKTLVQENGGPALAIQTKDGKTEHLPADRILVTVG
                     RKPCTEGWGLENMAIDMDGRFVRVDDQCRTSMRDVWAIGDLVGEPLLAHKGMAQGEMV
                     AEIIAGHRRRFDPVAIAAVCFTEPEIVSAGLAPGDEAAKTIDTMSAMFPFSANGRALS
                     MDAGDDGGFVRVLARRDDHRILGIQAVGAHVSELAGEFALALEMGAVLEDIAGTIHVH
                     PTLGEAFHETALRTLGHALHI"
     gene            complement(5756..7087)
                     /locus_tag="VG274_06120"
     CDS             complement(5756..7087)
                     /locus_tag="VG274_06120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWE06264.1"
                     /translation="MGRYVFKLPDVGEGTAEAEIVAWHIAVGDRIEEDQQLVDVMTDK
                     ATVEMTSPVPGVVVSLHGAPGEMATVGAPLVELDVEGAGNVDASAPKTSERPPRPSPL
                     PQGGGGSKKNDTDVASKGISLSPAAGEGRGQGDGTAKSAHAPTAAFATRAPGTKPLAS
                     PAVRQRAEEVGISLPFVPGTGPAGRIEHADLDAFIASGGRIANMRGGYAQREGIEEIK
                     VIGLRRKIAEKMQESKRHIPHFAYVEEIDMTELENLRAHLNRTRADGQPKLSLLPFLM
                     RGLVRLLPSWPQINARFDDEAGIVHRHAGVHIGIATQTANGLIVPVVRHAEALDIWQA
                     AAEVARLAQLTRDGKASREELTGSTITITSLGALGGVVTTPVINRPEVAIIGPNAIVV
                     RPVVREGQIVVRKMMNLSSSFDHRVVDGYDAAEFIQRLKALLEHPATLFMD"
     gene            complement(7089..8102)
                     /locus_tag="VG274_06125"
     CDS             complement(7089..8102)
                     /locus_tag="VG274_06125"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWE06265.1"
                     /translation="MATMNMIQALNSAMDVMLGRDPDVLVFGEDVGYFGGVFRATDGL
                     QKKYGLTRCFDAPIGEGGIVAAAIGMGAYGLRPVVEIQFADYIYPAFDQIVSEAARLR
                     YRSAGDFTAPITIRTPYGGGIFGGQTHSQSPEALFAHVAGLKTVIPSTPYDAKGLLIS
                     SIEDDDPVIFLEPKRIYNGPFDGHHDHPVSPWSQHPASEVPENYYNVPLGKAAVLRKG
                     NDLTLLAYGTMVHVGLAAANESGIDAEVIDLRTLVPLDAETIVQSVEKTGRCVIVHEA
                     TRTAGFGAELSALVQENCFYHLEAPIERVTGWDTPYPHAFEWQYFPGPARLIEAMRRA
                     MGD"
     gene            complement(8105..>8727)
                     /locus_tag="VG274_06130"
     CDS             complement(8105..>8727)
                     /locus_tag="VG274_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWE06266.1"
                     /translation="RIAAAWIGEGATAEGDFHSALTFAAVYRAPVILNIVNNQWAISS
                     FEGIAGGEQATFAARAIGYGLPGLRVDGNDFLAVFAATQWAVERARANLGATVIELYT
                     YRAEGHSTSDDPSRYRPADEAKCWPLGDPVVRLKAHLIANREWSDDDHVKAEKEAAET
                     VRAANREAESFGTLGTGSVVSVKTMFEDVYKDVPLHLRRQRQQLGV"
BASE COUNT         1561 a         2820 c         2778 g         1568 t
ORIGIN      
        1 ggccgatctt ctcgacatga ttccattcct gcgcgcattt tcacgatcct tgtgcggcaa
       61 tcaggaaatg gccgacgatc ttgcccagga gactttggtg aaggcctggc aggcgcgcaa
      121 tatgttcgca cctggaacga acctcaaagc ctggctcttc acaatcctgc gcaaccagtt
      181 ctactcggac cggcggcgcg cgtggcgcca ggcgccgtgg gatcaggacg ccgcggaacg
      241 catccccgga tcgagcgccg aacaggcctc ggcggctgaa ttgtccgata cggcgcgtgc
      301 gctctcgcgt ctttccgacg agcagcgcga agcgctgatt ctcgtcggcg cgggggggtt
      361 ctcctacgag gatgccgcgg cgatatgccg ttgcgcggtg ggtacggtga aaagtcgcgt
      421 cgcgcgggcg cgcaaatcgt tgttgtcgat tctcgagggc gaagatccat tgccgccggc
      481 gtcgcacggg gcggaaggag acgccgcaaa tgagattatg gcggaactgg atcgcctctc
      541 gcccggagcg tcgcagggtg tggtggcacg acggcggacc aagggagcca catcggccta
      601 gcggctggtc tctgcgtctg cggctactcg tcatcgtaac gatcgcgctg ctgcccatcg
      661 cgtttgcggg cttcgtgcag gggctcgacc gcgcccgccg cgacgttgca caaattcgcg
      721 aacgactcgt cgccgacgcg cgcctcagcg ccgcgcccga acagaacgtg ctcgcggcag
      781 ccgaacatat tgggcgcgtc ctcgcgagcc tgccgcaggt gagcaacgcc acagattcct
      841 gtgaggacga actcgcttcc gcactcaaag gcatttcctt cataagcaac gcatcgcgca
      901 tcgatcgccg ggggcgcgtg gcgtgttctg cccatcccgc agcaggcgga ctcccggccg
      961 gcaatctttc gaactggcgc gcgcttcggt ccgacgcgga ttttcagatc agcggacaga
     1021 gccacagcac cgtcaccggc gagccaatcg tgctcgtcag gctgccgctg cgcgacgctg
     1081 cgggacaatt cgatggcgca ctcgacatgg aaattgaagc gcgatggttc gacgacatga
     1141 ttcgtcgcag caggctgccg cggggatcgg tcgttgcgct tttcgatcgt tcgggcgcga
     1201 tcatcgcgtc gaacgatccg gcgcgcgcca tccgcctgtt tccgcgcgga tcgttgatcg
     1261 gcaacgcgga aaccgtccac gtggccagtg accgtcaggg caagaactgg atctacgccg
     1321 ctgcgccatt gctcgccgaa aacgtcttcg tgggcttcgc cgtacccgaa gcgggcgcgt
     1381 tgcgcccgac ccacatccgt gtggccaccg atttcagtct cccattcgcg atgctggcgt
     1441 tgagctggct ggcaatctgg atcgcgacgg agaggcaggt cacccgctgg atcgtctacc
     1501 tgcgccgtat cgcgacggcc tatcgcgccg ggcactacgc gttgaggcca aagctcgcgg
     1561 gcgcctcccg cgagttccgc gtgcttggcg attcgctttc gacgatggcc gattcgatcc
     1621 aggagcgcga tcgctcgctg cgcgaggcgc tggcgcaaaa atcgcttctg atcaaggaag
     1681 tgcatcatcg cgtgaagaac aatatgcaga tcgtcatgag cctgctgagc ctgcaggccg
     1741 gccggcttcg cgacccggcg gcgcaggacg cgctgcgcca ggcacggggg cggatcaacg
     1801 ccctcgcgct tgttcaccgg attctctacg agatcgaaga ccagcaaagc gtggacatca
     1861 agcctctgct cgagcaactc gcggaccaga cccgcgaggg gttcggaggc gagcgccgcg
     1921 aaatccgctc ggtcgtcgaa gccgaatctc tcgaactgcc ggcggagagt gcagttccgg
     1981 ttgcgctttt cgtcgtcgaa gcactcacca acgcattcaa gcacgccttt ccccttgggc
     2041 gcggcggcac cgtcgcgctt tgcctgcatc gcctcgatag cgacacgctg aagctcgcgg
     2101 tcaaggatga tggaatcggg ctcgatgagt cacgcctcga aaccagcatc ggcggccgtc
     2161 tgatttccac gttcggacaa cagctcggcg gaagggcgca gattcactcg tccgggagtg
     2221 gcgggacggc cgtcgaactg atatttcccc aacctgtggc aacgggagag tatccgcgcc
     2281 cggctctgcc gaaggcggat gccgcagagt gagtgtggtc agcttttccg gcggcgcttg
     2341 cgtgtcgcgg ccgctttcct ggccgaggcc gatcgcgcgg cagccggccg ttttgccgac
     2401 gccctgccgc cgagcttgcc gccctttcgc gaaggagcgc gattgacgcg cttgccccgg
     2461 ccggaggcgc tcttctttcc cccgcccgtc tccttgttga ccgtcgacca ggcgcggcgc
     2521 tcggcttcat cctcgccgat gccgcgttcc tcgtagcctt cttcgatatg gctggccatg
     2581 cgttgttgct tcgccgtgta gctcgacttg tctccgcgag gcattttttg catctccttc
     2641 cgaagccgca cccgtgccat tgcaaacgcg gtttgcagag cacggttgcg cgggcgcgcg
     2701 catcgaacgc aatgcgccgc ccgcgtagga atcgcagttg cgaatcagtc aagcggcggc
     2761 ggaagcgctt caggcgcgct ctgcaacagc cgtagcgaac tgaccgcggc cgcggcgagg
     2821 gcaaagcccg cggcgatgta gagcgcatag gccgttgcgt gcgcggcaat ctgcccgaag
     2881 aggagcgcga cgagcgctgc gcccatcgtc tggccgagca gccgcgccgt gcccagcatt
     2941 ccgctcgccc cgccgcttcg atggcgtggc gcagatccga tcagcgcgcg attgttgggc
     3001 gattggaaaa acccgaaccc gagcccgcac agagccatcc gccaggcaac gtccaccaat
     3061 tgcggacgct ggggaagaag tgcaagcgcc accagtccgc acgcgaaaat caacagaccg
     3121 atggcgccga gaaggcctgc cggatgacgg tccgccaatc ggccggcgac aggcgcggca
     3181 aatgcgatcg caagcggcca cggcgtgatc agcagccccg tctgcacggc cgaatagccg
     3241 aattcctgct gcaggtggaa cggcagcgcg acgaacgcca gcatctgcgc ggcgaaggag
     3301 cagatcgatg tcccgaccga aagcgcgaag atgggaatgc caagcaggtc gaccgggaac
     3361 aatggcgccg ggcgcgcaag ttcgcggcga acgaggaaat aaccgcagat aattgcgagc
     3421 gtcagctcaa ccgccacgag cttgccggcc tcgccgtgcc cgcccgcatc gatgcctccg
     3481 atcaggaaac cgaacgtgcc cgcgctgagc agggcgccag caaaatcgaa gccgtgcttg
     3541 ccaagcggcg tttttggcag cgcacgggcg gcaatcgcga cagcgaggat tccgattggc
     3601 acgttgaccg caaagagcca gggccaggaa gccaccgaaa ggatgccgct cgcgatggtc
     3661 gggccggcgg cggcggatat cgccaccacc accgtgttga ttccgatccc gcggccgagc
     3721 tgcgactgag gatagatgaa gcggataagt gcggtgttga cgctcatgat tcccgcagcg
     3781 ccaaatccct gcagcacgcg tgcaagagca agctcagtga gcgtttggga cagcgcacag
     3841 gcgagcgacg cggcggtgaa gagcgccagt cccgcgatgt agattcttcg gtacccgacg
     3901 atgtcgccga gcgaggacag gggcagcagc gagatcgtga tcgccagctg ataaccgttc
     3961 accacccaga tcgagaacgc agcgtcggtg tggagatcgc gcgcgatcgt gggcagcgcg
     4021 acattggcga tggcgctgtc gagcaccgac atggtgatgc cgagcgcgac cgtcgcgatc
     4081 gcccagttgc gctgcggtag cggcagaccg tcgggagaat gggtcatctt ttgccggaga
     4141 cacggattgc ttttgcaaga gatattgtca ttcccgcagg agcgctattg catctacaca
     4201 tcatgaattt ccccaaaaat tcgtcatcgc cggggggccg gccacgacga aactcggagg
     4261 atgtgtagat accttggcgc aggcgcggga atgacacgtg cacatggcga gtgaactcaa
     4321 tagtcgattc gcgtgctcag atatgcagcg cgtggccaag cgtgcgaagc gcagtctcgt
     4381 ggaacgcctc gcccaatgtc gggtggacat ggatggttcc cgcgatgtct tcgagcacgg
     4441 cgcccatttc gagagcaagg gcgaattcgc ccgcgagctc ggagacatgc gcgcccaccg
     4501 cctggatgcc gagaatgcgg tggtcgtcgc ggcgcgccag cacgcgcacg aagccgccgt
     4561 cgtcgcctgc gtccatcgac aaagcgcggc cgtttgccga gaagggaaac atcgcgctca
     4621 tcgtatcgat ggtcttggct gcttcgtctc ccggagcgag accggcgctg acgatttcgg
     4681 gttcggtgaa gcagacagcc gcgatcgcca ccggatcgaa acggcgtcgg tgaccggcga
     4741 tgatttcggc caccatctcg ccctgcgcca tccccttgtg cgcaagcagc ggttcgccca
     4801 cgagatcgcc gatcgcccac acgtcgcgca tcgaggtgcg gcactgatcg tcgacgcgca
     4861 cgaagcggcc atccatgtcg atcgccatgt tttcgaggcc ccagccttcg gtgcatggct
     4921 tgcgtccgac agtgacgaga atcctgtcgg ccggcagatg ctccgtcttt ccatcctttg
     4981 tctggatggc gagcgccggt ccgccgttct cctgcacgag agttttggcc tgagcgccca
     5041 gatgaacggc gacgcccgcg cgctgcagcc acctgttgac gggcgccgca agctccgcat
     5101 cgaagagcgg cagaatgcgg tcgagcgctt cgactaccgt cacctgcgat ccgagtttcc
     5161 ggaaagcaat tcccagctcg agaccgatat agcccgcgcc gacaacgacg agcttctgcg
     5221 gcagcgcgtc gagcgagaga gcttcgctcg aagagatcac cggtccggcg aagggaagga
     5281 agggcaattc cgtcgcgacc gagccgttgg cgaggatgac gtgctccgcc gtgatcgtta
     5341 cgctattggt cccgtcttcg accatgcagg tcttcgcgtc ggtgaatcgc gcccaacccg
     5401 caataacgcg caccttcgcg cgcttgcaca acgtcgcgac gcccatgctg agtttggtga
     5461 cgatgccttc tttccagcgg accagctccc tcatgtcgag gttgggcgga gacgcgagcg
     5521 aaatcccgtg gcggcccttc ttgcctgagg cgcgcgcgat ggcttcgaac tcgcctgcta
     5581 cgtgaatcat cgccttcgag ggaatgcagc cgcggatgag gcaggtgccc ccgactctgt
     5641 ccgcctcgac cagaatcgtg tcgagcccga gctggcccgc gcggatcgcg gccacgtaac
     5701 cacccggtcc gccgccgatg acgaggactt tggtcttgat cgtttcggcc atgagtcagt
     5761 ccatgaacag cgtggcggga tgttcgagca gcgctttgag ccgctggatg aattccgccg
     5821 cgtcgtaacc atcgacgacg cggtgatcga acgacgaaga cagattcatc atctttcgca
     5881 cgacgatctg tccctcacgg acgacagggc gcacgacgat cgcattcggc ccgatgatgg
     5941 cgacttcggg acgattgatg accggtgtgg tgacgacgcc gcccagcgcg ccgaggctcg
     6001 tgatggtgat cgtcgatccg gtcagctcct cgcgggaagc tttgccgtcg cgcgtcaatt
     6061 gcgccagccg cgccacttcg gccgcagcct gccagatgtc gagtgcctcg gcatggcgca
     6121 cgaccggcac gatcagaccg ttcgcggtct gagtcgcgat gccgatgtgc acgccggcgt
     6181 ggcgatgaac aattccggcc tcatcgtcga agcgggcatt gatctgcggc caggacggaa
     6241 gcaggcgcac caacccccgc atcaggaacg gcagcagtga aagcttgggc tgcccatccg
     6301 cacgcgttcg attgagatgc gcgcgcagat tctccaattc cgtcatgtcg atttcctcga
     6361 cataggcaaa atggggaatg tgacgtttcg attcctgcat cttctccgcg atcttgcggc
     6421 ggagcccgat caccttgatc tcttcaatgc cttcgcgctg ggcgtatccg ccacgcatat
     6481 tcgcaatgcg cccacccgag gcgatgaatg catcgagatc ggcgtgctcg atgcggccgg
     6541 ctggtccggt gccgggcaca aagggcagcg agatgcccac ctcctccgcg cgctggcgaa
     6601 cggcggggga agcgagcggt tttgttccgg gtgcgcgcgt tgcgaaggct gcggtcgggg
     6661 cgtgcgcgga tttcgcggtt ccatccccct gaccccggcc ctctcccgct gcgggggaga
     6721 gggagattcc tttcgaagcg acgtctgtgt cgttcttttt gctccctccc cccccttggg
     6781 ggagagggct ggggcgagga ggccgctccg aggtctttgg ggccgaagca tctacgttgc
     6841 cggcgccttc gacgtccagc tccacgaggg gcgcaccgac cgtcgccatc tcgcccggcg
     6901 cgccgtgcag cgacacgacg acccccggca ccggcgaggt catctcgacc gtcgccttgt
     6961 cggtcatcac gtcgacgagc tgctggtctt cctcgatgcg gtctccgacg gcgatatgcc
     7021 aggccacgat ctcggcctcg gcggttcctt cgccgacatc aggcagcttg aacacgtagc
     7081 gtcccatctc agtcccccat cgcgcggcgc atcgcttcga tcaatcgcgc cggccccgga
     7141 aaatattgcc attcgaacgc atgaggatag ggcgtgtccc agcccgtcac gcgctcgatc
     7201 ggcgcctcga gatggtagaa gcagttttcc tgcacgagcg cagaaagctc ggcgccgaag
     7261 ccggcggtcc gcgtcgcctc atgcacaatc acgcagcgtc ccgtcttctc caccgattgc
     7321 acgatcgtct ccgcgtcgag cggcaccagc gttctgaggt cgatcacttc cgcgtcgatc
     7381 ccgctctcgt ttgcggcggc aagccccaca tgcaccatcg ttccgtaggc aagcagcgtg
     7441 agatcatttc ctttgcgcag gacagccgcc ttgcccagcg gcacattgta gtaattctcg
     7501 ggaacttcac tcgccggatg ctgggaccag ggcgacacgg gatggtcgtg atggccatcg
     7561 aatggcccgt tatagattcg tttcggttcc agaaagatca ccggatcgtc gtcctcgatg
     7621 gaactgatca ggagtccctt cgcatcgtag ggtgtcgacg ggatcaccgt tttcaatcct
     7681 gccacatggg cgaacaatgc ttccggactc tggctgtggg tctggccacc gaaaatgccg
     7741 ccgccgtaag gcgtgcgaat ggtgatcggc gcggtgaaat cgcccgccga tcggtagcgc
     7801 agccgagccg cttcggacac gatctgatcg aatgccggat aaatataatc ggcgaactga
     7861 atctcgacga cgggacgcag tccgtaagcg cccatcccga tcgccgcggc aacgattccg
     7921 ccttcgccga tcggcgcgtc gaagcagcgt gtaagtccgt attttttctg cagaccgtct
     7981 gtcgcgcgga atactccgcc gaaatagccg acatcctcgc cgaagacgag cacgtcggga
     8041 tcgcgcccga gcatcacgtc catcgccgag ttgagcgcct ggatcatgtt catcgtcgcc
     8101 atactcacac gccgagctgc tggcgctggc ggcgcagatg caaaggtacg tccttataaa
     8161 cgtcttcgaa catggttttg acgcttacga cggagcccgt gccaagcgtg ccgaaacttt
     8221 cggcctcgcg gttggcggcg cgcaccgttt ccgccgcttc cttctcggct ttcacatggt
     8281 cgtcgtcgct ccactcgcgg ttcgcgatca gatgcgcctt cagccgcacg actggatccc
     8341 cgagcggcca gcactttgct tcgtccgcgg gccggtagcg cgaagggtcg tcgctcgtcg
     8401 aatgcccttc ggcgcgatag gtgtaaagct cgatcacggt cgctccgaga ttggcgcgcg
     8461 cgcgctccac tgcccattgc gtggcggcaa aaacagccag gaagtcattg ccgtcgacgc
     8521 gcaatccggg cagcccgtag ccgatcgcgc gcgccgcaaa cgtcgcctgc tcgccgccgg
     8581 cgataccttc gaagctcgat atcgcccatt gattgttgac gatgttgagg atgacgggcg
     8641 cgcggtagac agcggcgaag gtgagcgccg aatggaaatc gccttccgcc gtcgcccctt
     8701 cgccaatcca cgccgcggcg atgcggt
//
LOCUS       DASZMC010000299         7230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2876339,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000299 DASZMC010000000
VERSION     DASZMC010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876339"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..514)
                     /locus_tag="VG274_06135"
     CDS             complement(128..514)
                     /locus_tag="VG274_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE06267.1"
                     /translation="MIVVDASAAVALVLAEPVQGALAEKLASSEERAMSPISYSELVM
                     VLSRAHSHPKLIADAFVRDMRVMLLTIDRQQTELAVHAFLLYGKGRHPARLSLGDCFS
                     YAAAKALDSPLLFVGGDFSQTDVLVA"
     gene            complement(511..786)
                     /locus_tag="VG274_06140"
     CDS             complement(511..786)
                     /locus_tag="VG274_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWE06268.1"
                     /translation="MGLNIKSARAEAAIRELAAKTGEGLTEAVEKAVQERLERMKGPT
                     TSETAVHLLQRLRPIQEAVAAERRARRDKRTSRELMDELYDEHGLPK"
     gene            complement(889..1083)
                     /locus_tag="VG274_06145"
     CDS             complement(889..1083)
                     /locus_tag="VG274_06145"
                     /inference="COORDINATES: protein motif:HMM:NF033233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HWE06269.1"
                     /translation="MGGMIVVAIALAVVALILAAGLSTLWIGGDTQRRWSNRLMRYRV
                     LAQFVAILVILAVFYFARGH"
     gene            complement(1112..1552)
                     /gene="gloA"
                     /locus_tag="VG274_06150"
     CDS             complement(1112..1552)
                     /gene="gloA"
                     /locus_tag="VG274_06150"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HWE06270.1"
                     /translation="MRYLHTMVRVKDLDRSLYFYCELLGLKEIRRIDNEKGQFTLVFL
                     AAPEDAPRALAEHAPMVELTHNWEPEDYDGGRNFGHLAYYVDDIYAACEKLMEGGVTI
                     NRPPRDGHMAFVRSPDGISIELLQNGSPKAPEAPWNEMPNVGNW"
     gene            complement(1701..4340)
                     /gene="mutS"
                     /locus_tag="VG274_06155"
     CDS             complement(1701..4340)
                     /gene="mutS"
                     /locus_tag="VG274_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWE06271.1"
                     /translation="MMAQYLEIKAAHDGYLLFYRMGDFYELFFDDAVKAAAALDIALT
                     KRGRHLGSDIPMCGVPVHAAEQYLEKLIRKGFRVAICEQIEDPAEARKRGAKSVVGRE
                     VIRLVTPGTLTEDSLLEARSSNLLASLGRAAGEYALATADMSTGEFQVCAIAPAEFAA
                     ELARLAPRELLVPDALAGAEEFAELFRAMEPAPTPLPARNFDSAQGERVLKAHYGVAA
                     LDGLGAFTRTEISAAGALIGYLDLTQKGKLPTLKTLSRSSIDARMSIDAATRRNLELV
                     ETLSGQRAGSLLGTIDRTLTSAGARELASRLSSPLTDVAEISLRLDAVALFVAEQELC
                     AALRNDLRRTPDIARALARLSLQRGSPRDLGALRDGIRAGRALRGHLSILDDPLKPSP
                     GETRKAREAFTKGVAALSELSDRLEHLLVVEPPAYTRDGGFIAAGAHAALDEARALRD
                     ESRRVVAALEVRYRAESGVQPLRIRHNGVLGYFVELTPPNADRLTAGPMREIFRHRQT
                     IGGAVRFSTDELATLASRIAQSAEQALAIERELFDSLAAEAARASASLLAIADAIATL
                     DVATALAELAMTERHARPLVDESLAFEIVRGRHPVVEAALAQSRSGSFVPNDCNLSGE
                     GGGRIWLVTGPNMAGKSTFLRQNALIAILAQMGAFVPAESARIGVVDRLFSRVGAADD
                     LARGRSTFMVEMVETAAILNRATGKSLVVLDEIGRGTATFDGLAIAWAAVEHLHAANR
                     SRALFATHYHEMTALAGRLESVTCVTMRVSEWKDSIVFLHEVARGAADRSYGIHVAKL
                     AGLPSAVIARAEDVLRALEEGREGHKPLARIDDLPLFSALRSEIAPSRQSAVEVRLSG
                     VLPDALSPKAALELIYELKQLLK"
     gene            4305..6713
                     /locus_tag="VG274_06160"
     CDS             4305..6713
                     /locus_tag="VG274_06160"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HWE06272.1"
                     /translation="MRGLDLEILRHHGCGAPGLRGNGILHLHARIRADGPLKGEGRGR
                     DILWIGKHGQRMARPSFTQEEVLAFHAGGKPGKIEITPTKPMATQRDLSLAYSPGVAV
                     PVRVIAERPDAVFDYTARGNLVAVISNGTAILGLGDLGALASKPVMEGKAVLFKRFAD
                     VDAIDLEVDTSDVDAFVNCVRYLGPSFGGINLEDIKAPDCFVIEERLRELMDIPVFHD
                     DQHGTAIISAAGVINALHLTNRKLSDIKVVVNGAGAAGIACLELLKAMGLPNANAVLC
                     DTKGVVYRGRTIGMNQWKSAHAIETNARTLADALKDSDLFLGLSVKDAVTPEMVKTMA
                     KAPIIFAMANPDPEISPEAVKAVRPDAIMATGRSDYPNQINNVLGFPYIFRGALDVRA
                     KTINEAMKIAAAEALAALAREDVPDEVALAYRGARPAYGPEYIIPVPFDPRLVSRVSS
                     AVAEAAVKTGVARRESKDAETYRLELSARLDPSANLFQHITASVRANPKRVVFAEGEE
                     EAVIRAAAAFQSSGLGKALLVGREEVVRAGIHKLGVEDCGLEIRTPQSAKESTPYVDT
                     LYARLQRRGALYRDCLRMVRNDRNIFAACMLARGDADAMVTGVTRSYASALGDVRLVL
                     DVPKGKRPIGVLVIFMKGRVVFVADTSVHEIPTSSELAEIAVQAASVVKSFGFVPRVA
                     LLASSTFGFPRSERSERIVEAVHLLDQRGVDFEYDGEIAADVALDPGMLALYPFCRLS
                     ETANVLIMPAIHSASISTKLLQQIGGVTVLGPLLVGLERPVQIAPLGAKMSEIFNAAV
                     IAAL"
     gene            7067..>7230
                     /locus_tag="VG274_06165"
     CDS             7067..>7230
                     /locus_tag="VG274_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWE06273.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGATPKVIENAEGARTTPSIVTFTA
                     DGERLVGQSA"
BASE COUNT         1349 a         2280 c         2288 g         1313 t
ORIGIN      
        1 tcggtgctct ccagttcagt gacatatagt aattgggttt ctctggatct ttggcggcgg
       61 ggagtgggcc gtaaaattct ttggagcgat agtataattg cgcttttatc tcgctgtggt
      121 tgtcggttca tgcgacgagc acgtcggttt gcgagaaatc gcctccaacg aataacagcg
      181 gagaatcgag cgccttcgct gcggcgtaag agaagcaatc gccgaggcta agccgtgcgg
      241 gatggcgtcc ttttccgtag agcaggaacg catgcaccgc caattctgtc tgttgccgat
      301 cgatcgtgag cagcatcacg cgcatgtcgc gcacgaacgc gtcggcgatc agttttggat
      361 gcgaatgggc gcgggagagc accatcacaa gctcggaata ggatatgggc gacattgcgc
      421 gctcttccga ggaggcgagc ttttccgcga gagcgccttg cacgggttcg gccaggacca
      481 acgctactgc cgccgacgcg tcgacaacga tcatttcggc aggccgtgct cgtcataaag
      541 ctcgtccatg agctcccggg atgtgcgttt gtctcttcgc gctcgtcgct ctgccgcgac
      601 cgcctcctga atcggcctca acctttgtaa caggtgcacg gcggtttcgg aggttgttgg
      661 ccccttcatg cgttcaagcc gttcctggac cgccttttcg accgcctccg tcaacccttc
      721 cccggtcttc gcagcgagtt cgcggatcgc ggcctccgcg cgtgcgctct ttatgttgag
      781 acccattttt ctacctttct atatgagaag gtagaattat acccagaacc ggtcaatgcc
      841 aatgcctggg gcacggcgcc gctgcctacg ccgcctggtg gacgcggatc agtgccctct
      901 cgcaaaatag aacaccgcca aaattacaag aatggcgacg aactgggcga gcacgcggta
      961 gcgcatcagg cggttcgacc agcggcgttg cgtgtcgccg ccaatccaga gcgtcgaaag
     1021 tccggcggcg agaatcaacg ccacgacggc aagagcgatg gcgacaacaa tcattcctcc
     1081 catgcggtgt tactcttgcc cgggaacgcg ttcaccaatt gccgacattc ggcatctcgt
     1141 tccacggagc ttcgggcgct tttggactgc cgttctgcag cagctcgatc gatatgccgt
     1201 ccggggaacg tacgaaggcc atgtgaccgt cgcgcggcgg ccggttgatc gtcacgccgc
     1261 cttccatgag cttctcacag gccgcataaa tatcgtccac ataataggcg aggtgaccga
     1321 aattgcgccc accatcatag tcttccggct cccagttgtg ggtaagctcg accattggcg
     1381 catgttccgc aagcgcgcgg ggagcatcct cgggcgccgc cagaaacacc agggtgaatt
     1441 ggcctttctc attgtcgatg cggcgtatct ctttcaggcc gagcagttcg caatagaagt
     1501 aaagcgaccg gtcgagatcc ttcacgcgga ccatggtgtg cagatagcgc atgtcgtcgt
     1561 ctccgtttcg cccggagaat aagcgcagag cgcgccggac gctactactg gggaccatgg
     1621 ttcgacgggc gcaccgtgag gacctcaccc tgagcttgac gaaggataag gcgggaatcg
     1681 ttcgtcggcg acagctggcg ctacttcaac aattgcttca gctcataaat caactcaagc
     1741 gcagcttttg gcgacagcgc gtcgggcaga actccagaca gcctaacttc aaccgcgctt
     1801 tgccgcgacg gcgcaatctc ggaccgaagt gcggagaaca gcggcaaatc gtcgatgcgt
     1861 gccagcggct tatggccctc acggccctcc tccagagcgc gcaggacgtc ttccgcccgc
     1921 gcgatgacgg cagagggcaa tccggccagc ttcgcgacgt gaatcccgta ggagcgatcc
     1981 gcggccccgc gcgccacttc gtgcagaaac acgatcgagt ctttccactc gctgacgcgc
     2041 atcgttacgc aagtgacgga ctccaatcgt ccggcgagcg cggtcatctc gtggtagtgc
     2101 gtggcgaaga gggcacgcga gcggttggcg gcgtgcaaat gttcaaccgc tgcccaggcg
     2161 atggcgagcc cgtcgaaggt tgcggtgccc cggccgattt cgtcgagaac gaccaggctt
     2221 ttgccggtgg cgcggttgag gatcgccgcg gtctcgacca tctccaccat gaaggtcgag
     2281 cgcccgcgcg cgagatcgtc ggcggcgcct acgcggctga acaggcggtc gaccacgcca
     2341 atacgcgccg attccgccgg tacgaaggcg cccatctggg cgagaatcgc gatgagcgca
     2401 ttttgcctga gaaaggtcga tttgcccgcc atgttgggcc cggtgacgag ccagatgcgg
     2461 ccgccgcctt cgcctgaaag attgcaatcg ttgggcacga aacttccgga tcgcgattgt
     2521 gcaagggccg cttcgaccac cgggtgccgt ccgcgtacga tctcgaacgc gaggctttca
     2581 tcgacaagcg gccgcgcgtg gcgctccgtc atcgcaagtt cggcgagcgc agtggcgaca
     2641 tcgagcgtgg caatggcatc ggcgatcgcg agcagggacg cgctcgcgcg tgcggcttcc
     2701 gccgcaagcg aatcgaacaa ttcgcgctcg atcgcgagtg cctgctcggc agactgcgcg
     2761 atgcggctcg ccagcgtggc gagttcatcc gtcgagaatc gcaccgcgcc gccgatcgtc
     2821 tggcggtggc ggaaaatctc gcgcatcggg cccgccgtca gcctgtcggc gttgggcggt
     2881 gtcagctcga cgaaatagcc gagcacgcca ttgtggcgaa ttcgcagagg ctggacgccg
     2941 ctctccgcgc gatagcgcac ctcgagggcc gcaacgaccc ggcggctttc gtcgcgcaag
     3001 gcccgcgctt cgtcgagcgc cgcgtgcgcg cccgccgcga taaacccgcc gtcgcgagtg
     3061 taggcgggcg gctccaccac tagcagatgt tcgagcctgt cgctgagttc gctcagtgct
     3121 gcgacgcctt tggtgaacgc ttcgcgcgcc ttgcgcgtct cgcccggcga cggtttgaga
     3181 ggatcgtcga gaatcgacaa atgtccgcgc agggctcgtc ccgccctgat gccgtcgcgg
     3241 agcgcaccga gatcgcgcgg cgagccgcgc tgaagggaga gccgcgccag cgcccgcgca
     3301 atgtccggcg tgcggcgaag gtcgttgcgc agagcggcgc acagttcttg ttcggccaca
     3361 aacaaggcga ccgcgtcgag gcgcagagaa atttccgcga catcggtcag gggcgaggac
     3421 aaccggctcg ccagttcgcg cgcgcccgcc gatgtaagag ttcggtcgat cgtgccgagc
     3481 aggcttccgg cgcgctgtcc cgaaagcgtc tccacaagtt cgagattgcg tcgcgtggcg
     3541 gcgtcgatgc tcatgcgggc atcgatcgag gatcgcgaaa gggtcttgag tgtcggcagt
     3601 tttcctttct gcgtcagatc gagataaccg ataagcgctc cggctgcgga aatctccgtg
     3661 cgcgtgaacg cgcccaggcc gtcgagcgcg gcgactccgt aatgcgcttt caggactcgc
     3721 tcgccctgcg cggaatcgaa attgcgcgcg gggagtggcg tcggcgccgg ttccatcgcg
     3781 cgaaacaact cggcgaattc ctcggcgcca gccagggcgt cgggaacgag cagttcgcgc
     3841 ggagcgagcc gtgccagttc cgccgcaaac tcggccggcg caatcgcgca tacctggaat
     3901 tcgccggtag acatgtcggc cgtcgccaga gcgtattcgc cggccgcccg gccgagcgag
     3961 gcaagaaggt ttgacgagcg tgcttcaagc aacgaatcct cggtgagcgt gcccggagtc
     4021 accagccgga tcacttcgcg cccgaccacc gatttcgcac cgcgcttcct ggcttcggcc
     4081 ggatcttcaa tctgttcgca gatggcgacc cgaaagccct tgcggatcag cttctcgaga
     4141 tattgctccg ccgcatgcac cggcacgccg cacatcggaa tgtcgctgcc caggtgacgt
     4201 ccgcgtttgg tcagcgcgat gtcgagcgcc gcggccgcct tgaccgcgtc gtcgaagaac
     4261 agctcgtaga aatcccccat gcggtagaag aggaggtagc cgtcatgcgc ggccttgatc
     4321 tcgagatatt gcgccatcat gggtgtggcg ctcccggact ccgcggcaat ggaattcttc
     4381 atctccatgc gcgaatccgc gcagacgggc cattgaaagg cgagggccgg gggcgcgata
     4441 ttctgtggat cggcaaacac ggacagcgca tggcccggcc ctcattcacg caggaagaag
     4501 tgctcgcctt tcacgcgggc ggcaagcccg gaaagatcga gatcacgccg accaagccga
     4561 tggcgaccca gcgggatctc agcctcgcct attcgcccgg cgtggccgtt ccggtacgcg
     4621 tgatcgccga gcggccggac gccgtcttcg actacaccgc cagaggcaat ctcgtggcgg
     4681 tcatctcgaa cggaacggca atcctgggtc tgggcgatct gggcgcgctg gcctccaagc
     4741 cggtgatgga gggcaaggcc gttctcttca aacgctttgc cgatgtggac gcgatcgatc
     4801 ttgaggtcga caccagcgac gtcgatgcct tcgtcaactg cgtgcgctat ctgggcccca
     4861 gcttcggcgg catcaatctg gaggacatca aggcgcccga ttgcttcgtg atcgaagagc
     4921 ggttgcgcga actgatggac atccccgtgt ttcatgacga ccagcacggc accgccatca
     4981 tctccgcggc gggcgtcatc aacgcgcttc atctcaccaa tcgtaaattg tccgacatca
     5041 aggtggtcgt gaacggagcg ggcgctgccg gaatcgcctg cctggaactg ctgaaggcga
     5101 tggggttgcc gaatgccaac gccgtgctgt gcgacaccaa gggcgtcgtc tatcgcggcc
     5161 gcacgatcgg catgaatcaa tggaaatcgg cgcacgcgat cgaaacgaac gcgcggacgc
     5221 tcgccgacgc gctcaaggac agcgacctgt tcctcggcct ttcggtgaag gatgcagtga
     5281 ccccggagat ggtgaagacc atggcgaagg cgccgatcat cttcgcgatg gccaatccgg
     5341 atcccgaaat ctcgcccgag gccgtcaagg cggtgcgccc cgacgccatc atggccaccg
     5401 gccgctccga ttatcccaac cagatcaaca atgttcttgg cttcccctac atttttcgcg
     5461 gcgcgctcga tgtgcgcgcg aagaccatca acgaagcaat gaaaattgcg gcggccgagg
     5521 cgctggcggc gctggcgcgc gaggacgtac ccgatgaagt agcgctcgcc tatcgcggcg
     5581 cgcgccctgc ctatggtccg gagtacatca tccccgttcc cttcgatccg agacttgttt
     5641 ctcgcgtctc ctcggcggtt gcagaagcgg ccgtcaaaac cggcgtggcg cggcgcgagt
     5701 cgaaagacgc agaaacctac cgccttgagc tgtccgcccg gctcgatccg tccgcgaatc
     5761 tgtttcagca catcaccgcg agcgtgcgcg ccaaccccaa gcgcgtggtt ttcgcggaag
     5821 gcgaggaaga agcggtcatc cgcgccgccg ctgcgttcca gagctcggga ctgggcaagg
     5881 ccctgctcgt gggacgggag gaagtcgtca gagccggcat ccacaagctg ggcgtcgagg
     5941 attgcgggct ggaaatccgg acgccgcaat ccgccaaaga atccacccct tatgtggaca
     6001 cgctctacgc acgcctgcag cggcgcggcg ctctttatcg cgattgtctg cgcatggtca
     6061 ggaacgaccg caacatcttt gccgcctgca tgctggcgcg cggcgacgcg gacgcgatgg
     6121 tgaccggcgt cacccgcagc tatgcgagcg cactcggcga cgtgcggctg gtgctcgacg
     6181 tgcccaaggg gaaacggccg atcggcgtgc tcgtcatctt tatgaagggc cgcgtcgtct
     6241 tcgtcgccga cacgagcgtg catgagatac ccacaagctc ggagctggcg gagatcgcag
     6301 tgcaggccgc cagtgtggtc aagagtttcg gcttcgtgcc acgggtggcg ctgctcgcct
     6361 cctcgacctt cggtttcccg cgcagcgaac gcagcgaacg gattgtcgag gcggtccacc
     6421 tgctcgacca gcggggcgtc gatttcgaat atgacggcga gatcgccgcc gatgtggctc
     6481 tcgatccggg gatgctcgcg ctctacccgt tctgccggct cagcgagacc gcgaacgtac
     6541 tcatcatgcc ggccatccat tccgcatcga tttcgacgaa gctcctgcag cagatcggcg
     6601 gcgttacggt cctgggcccg ctgctggtcg gtctagaacg gcccgtgcag atcgctcccc
     6661 tcggcgccaa gatgagcgaa atcttcaacg ccgcggtgat cgcggcgctg tagggttccg
     6721 attccaacgc ttcgacccaa aaactgtcat ggcccgcgca agcgggccat agaagtctcg
     6781 gcaacggaac gatagcgcac gggaagttac gcagcctgca accagtagag ggcgaaccgc
     6841 gcaaggcaaa gcgacccgaa tgtcaatggg ctccggaccc tgccgcactg cgaataagct
     6901 gctgaaatca ggcttcgttc gccttgcgag gcgaaaatca cgcccctata taccccccag
     6961 acgcggtaag ggctttaacc cttcggccgc ggttgtttca tggggacgga ccggaggcgc
     7021 tccttgggga ggctagactc cgttgcccga acggaaggac cgaataatgg cgaaagtgat
     7081 tggcatcgac ctcggcacga cgaattcctg tgtcgcggtc atggaaggag caacgcccaa
     7141 agtcatcgag aacgcggagg gtgcgcgaac gaccccttcg attgtcacgt ttaccgccga
     7201 tggcgaacgc ctcgtcggcc agtctgcgaa
//
LOCUS       DASZMC010000300         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5066462,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000300 DASZMC010000000
VERSION     DASZMC010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5066462"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VG274_06170"
     CDS             complement(<1..285)
                     /locus_tag="VG274_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06274.1"
                     /translation="MSNARPLTIMLVCGEPSGDQIGAELMSGLRSLAGENLRLVGVGG
                     TAMAAQGLQSLFPLDDTAVMGLREVVPRIPVILRRVREAADYATRVRPDAV"
     gene            complement(289..1134)
                     /gene="lpxI"
                     /locus_tag="VG274_06175"
     CDS             complement(289..1134)
                     /gene="lpxI"
                     /locus_tag="VG274_06175"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HWE06275.1"
                     /translation="MSCLGIVAGGGELPAAVAQSARESGRDVFILGLRGSADSSIAQF
                     PHDWVSLGEAGSALRMLREHGCRDVLFAGRVARPRFSEVKVDAKAVLLLPRVISAARK
                     GDDALLRALVDILDAEGFRSVGIAEAAPGLLTPEGVLGKLNPDRESEADIAHGVKIVR
                     RLGALDIGQAAIVCAGLVLTVEAAEGTDAMIRRAAELPENIRGSSARRCGVLVKALKP
                     LQDGKTDLPVIGVKTIEQAARAGLAGIAIESRRTLIVERSAAIEAADAAGLFLYGFST
                     AAFAD"
     gene            complement(1131..1946)
                     /gene="lpxA"
                     /locus_tag="VG274_06180"
     CDS             complement(1131..1946)
                     /gene="lpxA"
                     /locus_tag="VG274_06180"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWE06276.1"
                     /translation="MTVHPTAIVEQGARLCGGVEIGPYCVVGGQATLCEGVRLHSHVV
                     IGGQTEIGAHTVIHSFTALGGGAQMRKSNPPGMGLQIGADCILRESVTISTGSAAGRG
                     VTTIGERCYLMAGSHVGHDCVVGNEVTLSNGVQLAGHVEVGEGVIMGGLSAVQQFGRI
                     GRNAFISGLSGVAADVIPYGSAIGLHVRLGGLNLVGLRRRGIARENIHALRAAYGMIF
                     EADSGTIHDNARRAGECWPGIPEVQEVVTFILADAKRPICPARSRRREPSESE"
     gene            complement(1918..2412)
                     /gene="fabZ"
                     /locus_tag="VG274_06185"
     CDS             complement(1918..2412)
                     /gene="fabZ"
                     /locus_tag="VG274_06185"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWE06277.1"
                     /translation="MTAARDITLDVDQIKRLLPHRAPFLFVERLAEIRPGESAVGYKA
                     VSYNEPYFQGHFPEYAVMPGVLIVEAMAQTAGALVVHSLGLTRGRRTVYFMTIDKARF
                     RRPVRPGDLLRIPVKAVRRRGPVWKFTGEAFVGDELCAEAEFGAMIHDNQPDPDDRPS
                     DGDR"
     gene            complement(2414..3448)
                     /gene="lpxD"
                     /locus_tag="VG274_06190"
     CDS             complement(2414..3448)
                     /gene="lpxD"
                     /locus_tag="VG274_06190"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWE06278.1"
                     /translation="MADPRFYDNRGPFTLAKVCENASVPLPEADGGSQIADLATLEGA
                     GPSHLTFCAAKDVVLALAASRAGFCLVAEGAALPTARLTTRPLPCHSVQHAFAAAAEL
                     FYPEHNGTAWIQDRAVHPSARIGDNVILAPGVVVGPNAEIGAGTHVGPNAVIGRGVAV
                     GRDCDIGSHVAISHAYLGDSVIVLPGAQIGQAGFGFAGSKEGHVRIPQLGRVIVQDRV
                     EIGACTTVDRGALGDTVIGDGTKIDNLVQIGHNTRIGRHCVIVAQTGISGSCELGDFV
                     VLGGQVGISDHVHIGDGARFAARGAATPGVYPGGQDYGGVPARPMKQWRREVAAVARL
                     AKRGRQVRDG"
     gene            complement(3487..4122)
                     /locus_tag="VG274_06195"
     CDS             complement(3487..4122)
                     /locus_tag="VG274_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HWE06279.1"
                     /translation="MGKSILTIRIALVAAGLASMLATTGLAVAQGKPHPPAAGAAGGA
                     LPAPRIIVVDRSAILRASKVGQDIVRQVNGLTQSAESQFRAENEGLVKESQSLQQQIA
                     ILAPDVRSQKIRAFQSKEAAFKQKVQTRQDQIQGGLLRARQQVEAALAPILQGIMQER
                     GANLLLDRAAVVLGMVDVDVTRVTIQRLDQKLPSVKVQLVNSPPGLAQQMR"
     gene            complement(4127..>4714)
                     /locus_tag="VG274_06200"
     CDS             complement(4127..>4714)
                     /locus_tag="VG274_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWE06280.1"
                     /translation="SFEFSQDFAGFGGNTKYIRSLVASGIHHPLLWDAFVGTLTVNAG
                     YIEPYNNQPVRLTDRFYKGGDSFPGFALAGVGPREVSAGGANAIGGDAYAIGDADLRL
                     PEFLPADYGVALSLFSYFGTMGHLTSGLPADNCATFPPGLVCIKDNMAFRASAGIGVN
                     WKSPFGPIQISFAVPYVKESYDKSQVIYFSAGTGL"
BASE COUNT          897 a         1571 c         1423 g          823 t
ORIGIN      
        1 aaccgcgtcg ggccgaacgc gcgtcgcata atctgcggcc tcgcgcacac gccgcagaat
       61 gacgggaata cgtggcacga cttcgcgcag tcccatcacg gcggtgtcgt ccagcggaaa
      121 gagcgattga agcccttgcg ctgccatcgc ggtgccacca actccgacca ggcgaagatt
      181 ctcacccgcc agcgatctga ggccggacat caattccgcc ccgatctggt cgccggacgg
      241 ctcgccgcac accagcatga tcgtcaacgg acgggcgttc gacatggttc agtcagcgaa
      301 ggcggcagtc gaaaacccgt acagaaacaa tcctgcggca tcggcggcct cgattgccgc
      361 gctcctctcc acgatcaggg tacgcctgct ctcgatagcg atccccgcca agccggcgcg
      421 cgccgcctgc tcaatcgtct ttacgccgat caccggcaaa tccgtcttgc cgtcctgcaa
      481 tggcttcagc gccttgacga gcacgccgca tctgcgggcg ctgctgccgc gaatgttttc
      541 cggaagctcg gccgcgcgcc ggatcatcgc atcggttccc tccgcagctt cgaccgtcag
      601 cacgaggccg gcacagacaa tcgcagcctg cccgatatcg agcgcgccaa gccggcgcac
      661 gatctttacg ccatgcgcga tgtccgcttc gctttcgcga tccgggttca actttccaag
      721 cacgccttcg ggggtcagca agccgggcgc agcctcggcg attcccacgg aacgaaatcc
      781 ttcggcgtcc agaatatcca cgagcgcgcg cagcagcgcg tcgtcgcctt tgcgcgccgc
      841 cgagatcacc cgcggcaaca gcaggacggc cttggcatcc acctttactt ccgagaagcg
      901 cggccgcgcc acgcgccccg cgaacaacac atccctgcag ccatgctcgc gcagcatgcg
      961 caacgcgctg cccgcttcgc cgagcgatac ccaatcatgg gggaactgcg cgatggaaga
     1021 atcggctgag ccgcgcaagc cgaggataaa aacgtccctt cccgattccc gcgcgctctg
     1081 ggccactgcg gcgggaagct cgccccctcc cgcaacgatt ccgagacagc tcattcggat
     1141 tcggaaggct cgcggcggcg actgcgcgcg gggcagatcg gccgctttgc gtccgcaagg
     1201 atgaacgtca cgacctcctg cacctccgga attccgggcc agcattcgcc ggcgcgccgc
     1261 gcgttgtcgt gaatggttcc ggaatccgct tcaaagatca tgccataagc cgcgcgcagc
     1321 gcgtgaatgt tctcgcgtgc tattccacgg cggcgcaatc cgacgagatt gagtcctccg
     1381 aggcgcacgt gaaggccgat tgcactgccg tagggaatga cgtcggccgc cacgccggac
     1441 aggccgctga taaacgcgtt gcgtccgatg cggccgaact gctgcacggc cgaaaggcca
     1501 cccatgatca cgccctcgcc aacctcaacg tggccggcga gttgaacgcc attcgaaagt
     1561 gtcacctcgt ttccaaccac gcaatcatgc ccgacatgcg aacccgccat gaggtagcat
     1621 cgctcgccga tcgtcgttac gccgcgaccc gcggcgctgc ccgtgctgat cgtaacggat
     1681 tcgcggagga tgcaatctgc gcctatttgc aagcccattc ctggcggatt ggatttgcgc
     1741 atctgcgcgc cgccgccaag tgccgtgaag gaatgaatga ccgtgtgcgc accaatctcc
     1801 gtttgaccgc cgatgacgac gtgagaatga agtctgacac cttcgcagag cgttgcctgg
     1861 ccgccgacga cgcaataggg gccgatttcc acgccgccgc agaggcgtgc tccctgttca
     1921 acgatcgccg tcggatggac ggtcatcggg atcgggttgg ttgtcatgga tcatcgcgcc
     1981 gaattcggct tcggcgcaca attcgtctcc gacaaaagct tcgcccgtga atttccagac
     2041 aggcccccgg cggcgcaccg ccttgacggg aattcgcagg aggtcgcctg gccgcacggg
     2101 cctgcgaaac cgcgccttgt cgattgtcat gaagtagacc gtgcgcctgc cgcgtgtgag
     2161 tccaagagaa tggacgacga gcgcgccggc ggtctgcgcc atggcttcca cgatgagcac
     2221 gcccggcatc accgcatatt cgggaaaatg gccctggaag tagggttcgt tgtaggacac
     2281 ggccttgtat ccgaccgcgc tctcgccggg ccggatctcc gcgagccttt ccacgaagag
     2341 aaaaggcgcg cgatgcggca ggagccgctt gatctggtcc acatcgagcg tgatgtcgcg
     2401 tgccgcggtc atgctatcca tcccgaacct gcctgccgcg cttggcgagt cgtgcaactg
     2461 ccgcgacctc gcgacgccat tgcttcatgg gacgcgccgg cacgccgcca taatcctgac
     2521 ctcccggata gacgcccggc gtcgccgcgc cgcgggcggc gaacctggcg ccatcgccga
     2581 tgtgcacgtg atccgaaatg cccacctgac ctcccagcac aacgaagtcg cccaattcgc
     2641 aactgccgga aatgcccgtc tgggcgacga taacgcagtg tcgaccgatc cttgtgttgt
     2701 gcccaatctg caccagatta tcaatcttgg tgccatcccc gatcaccgtg tcgccgagtg
     2761 caccgcgatc gaccgtcgtg caggctccga tttcaacgcg gtcctgcacg atgacgcggc
     2821 cgagctgcgg aattctcaca tgaccttctt tggagccggc gaagccaaat cccgcctgtc
     2881 cgatctgggc ccccggaagc acaatcacgc tgtcgcccag ataggcgtga gagatcgcaa
     2941 catggcttcc gatgtcacag tcgcggccga cggcgacgcc acggccgatg acggcattgg
     3001 gtccgacatg cgtaccggcg ccgatctcgg cgtttgggcc aaccaccact ccaggcgcca
     3061 ggatgacatt gtcgccgatc cgcgcgctcg ggtggacggc gcgatcctgt atccaggccg
     3121 taccgttgtg ctcgggataa aagagctccg ccgccgctgc gaatgcgtgc tggacggaat
     3181 ggcatggcag gggtcgcgtc gttagccgtg cggtcggcag cgccgcgccc tcggcaacca
     3241 gacagaatcc cgcgcgagaa gcagcaaggg cgagcaccac atccttcgcc gcgcagaatg
     3301 tcaggtgcga agggccggcg ccctccaacg ttgcaagatc ggcgatctgg ctgccgccat
     3361 cagcttccgg caaaggaaca gaggcgttct cacagacttt cgcaagcgtg aacggcccgc
     3421 gattgtcata gaagcgagga tcagccatgg cctctcgaaa cggcgcgacg atagccgcgc
     3481 gacaactcag cgcatttgct gcgcgagccc gggcggcgaa ttcacgagct gcaccttgac
     3541 ggacggcagc ttctgatcaa ggcgttggat cgtcacccgg gttacgtcca catcgaccat
     3601 gccgagcacg acagcagccc ggtcgagaag aagattggcg ccgcgttcct gcatgatgcc
     3661 ttgcaggatg ggggccaaag ccgcctcgac ctgttggcgt gccctgagca ggccgccctg
     3721 aatttgatct tggcgcgtct gcaccttttg cttgaatgca gcttccttcg attgaaacgc
     3781 cctgattttc tgcgaccgca cgtcgggcgc gagaatggcg atctgctgct ggagcgactg
     3841 cgactccttc accaatcctt cgttttcggc acggaactga ctctcggcgc tctgcgtgag
     3901 cccgttgacc tggcgaacga tgtcctgtcc gaccttggag gcacgaagaa ttgccgagcg
     3961 gtccaccacg ataattcgcg gtgccggcag cgcgcctccg gcggcacccg cggccggcgg
     4021 atgcggcttg ccctgtgcga ccgccagacc tgtcgtcgca agcatggaag caagtccagc
     4081 cgcgaccaga gcgatccgga tcgtgagtat cgattttccc atgagtttag agtcctgttc
     4141 ctgcgctgaa atagatgacc tgcgacttgt cgtagctttc cttgacgtaa ggcacggcga
     4201 aggaaatctg gatgggcccg aagggcgatt tccagttgac gccaatgcca gccgaggcac
     4261 ggaatgccat attgtccttg atgcacacaa ggcccggcgg gaaggtggcg caattgtcgg
     4321 ccggcaatcc cgacgtgaga tggcccatcg tgccgaaata gctgaacagc gacagggcga
     4381 caccataatc cgccggcagg aattcgggga gccgcaggtc ggcgtcgccg atcgcgtagg
     4441 cgtcgccacc gatggcattt gctccgcccg cgctgacttc gcggggcccg acgcccgcga
     4501 gcgcaaaccc cgggaaactg tcgccaccct tgtagaagcg atcggtgaga cggacaggct
     4561 ggttgttgta gggctcaata tatccggcat tcacggttaa cgtgccaacg aacgcgtccc
     4621 acaacagcgg gtgatgtatc ccgctcgcga ccagcgatcg gatatacttc gtgttgccgc
     4681 cgaatccggc aaagtcctga ctgaactcga aact
//
LOCUS       DASZMC010000301         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4258610,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000301 DASZMC010000000
VERSION     DASZMC010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4258610"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VG274_06205"
     CDS             <1..477
                     /locus_tag="VG274_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWE06281.1"
                     /translation="VRLVDTTSIEASVQIPIETASYLREGLRVTVEIEGKRSTATVRA
                     IVPVGDVASRTIEVRLTLPSGTGFVGDAARIFIPSAQPRDVVAVPRDALVLREDNTYV
                     FKVDRKGVAQRVAVETGSEDGALVEIKGPVAPGERVIVRGAERLEAGQKVRPILAS"
     gene            844..>2146
                     /locus_tag="VG274_06210"
     CDS             844..>2146
                     /locus_tag="VG274_06210"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWE06282.1"
                     /translation="MPALILVALQIYSAAGIVPGSWRTQGQVSHTLHVIDAARALDQS
                     MSETERGEFGYIVTGDKTYLETYQNAARRTPRNLQRLRQLTLDNPSQVRRIAVLESAV
                     DLKLAELQTAVEARQKSGFLAAYRLIEANLKPDTRRVITGLVELIIASEDKLLAGREA
                     KFAALENSVENANTIGIALTISLMLLGALMLGRALRHDATRLARLRESEERFRHLVSG
                     VRDYAIFMLDPRGRVVSWNEGATRIKGYSPREAIGLHVSAFYPPEAGDRRAEADDELA
                     IAEREGGTAAEGWRVRKDGSRFYANTVTTALRAEDGTLRGFAKITRDVTERRAQQAAL
                     EESRSALAQAQKMEALGQLTGGLAHDFNNLLSVIIGGIEFAQRHSDTGDSQRSAQMLD
                     AAKQAAQQGAALVRRMLAFSRRQKLAPRVVDVNSLVRNMSEL"
BASE COUNT          422 a          650 c          694 g          380 t
ORIGIN      
        1 gttcgcctcg tcgacacgac ttccatcgaa gcgagcgtac aaattccgat agagaccgcg
       61 agttatctgc gcgagggcct gcgggttacc gtcgaaatcg aaggcaagcg ttcaacagcc
      121 accgttcgtg cgattgtgcc ggtcggagat gtggcgagcc gcacgatcga ggtgcggctg
      181 acacttcctt ccggcacagg ctttgtcggc gatgccgccc ggatcttcat accgtcggca
      241 caaccccgcg atgtggtcgc cgtgccgcgc gacgcgctcg tactgcgcga ggataacacc
      301 tacgtgttca aggtcgaccg caagggcgtg gcccagcggg tggcggtcga aaccggctcg
      361 gaagacggcg ccctggtcga gatcaaggga ccggttgcgc ccggcgagcg ggtcatcgtg
      421 cgtggcgccg aacgcctgga agccggccaa aaggtacggc ccatcctggc ttcgtaagag
      481 tacacacttc aatctggaag ttgtcatggc ccgcgcaagc gggccaccag atggtagtga
      541 cgtatcggtc aattcgctaa gacatttcgc gcacgatgca gcgctgaacg ggtttcaact
      601 gggtggcccg cttgcgcggg ccatgacaag tctttgattg acatgccgga atggcgcatt
      661 tccgtcccgg ccaataatcc accgcttgcc cgcccccgcc atcgcgctat gcttcaacag
      721 ggctgaggcc cggtcgagga tgcggcagcg gacacggtgt gttcgcgcgc ctacggattt
      781 ggggctgtgc cgatatttcg caaactgccc agtgcggtgt ggcaagggat cattctgatc
      841 tcgatgccgg cattgatcct ggtcgcactt caaatctaca gcgcggcggg aattgttccc
      901 ggttcgtggc ggacccaggg acaggtttcg catactttgc atgtgatcga cgccgcgcgc
      961 gcgctcgacc agtcgatgag cgaaaccgag cgcggagagt tcggctatat tgttacgggc
     1021 gacaagacct atctcgaaac ctatcagaat gctgcccgcc gcacgcccag gaacctgcaa
     1081 cggctgcgcc agctcactct ggacaatccc tcccaggtgc ggcgcatcgc agtcctggaa
     1141 tcggctgtcg acctcaaact cgccgagctg cagaccgccg tcgaggcgcg acagaaatcg
     1201 ggcttcctgg cggcgtatcg gctgatcgag gcaaatctca agccggatac gcggcgggtc
     1261 attaccgggc tggtcgagct cattatcgca tccgaggaca aacttctcgc agggcgcgag
     1321 gcgaagtttg cggcgctcga gaacagcgtg gagaacgcga acacgatcgg catcgcgctg
     1381 acgatttcgt tgatgctgct cggtgcgctg atgttgggcc gcgccttgcg ccacgacgcc
     1441 acgagactcg cgcggcttcg cgaaagcgaa gagcgattcc ggcacctggt atccggggtc
     1501 cgggactacg ccattttcat gctcgatccc agaggtcgcg tcgtcagctg gaacgagggc
     1561 gccacgcgca tcaaaggcta cagcccacgc gaagcgatcg ggcttcacgt gtccgccttt
     1621 tatcctcccg aagccggaga ccgtcgggcc gaagcggatg acgagctcgc gatcgccgag
     1681 cgcgagggcg gcacggcggc ggaaggctgg cgcgtgcgaa aggacggatc acgcttttat
     1741 gcaaacaccg tcaccaccgc gctgcgtgcg gaagacggga cgttgcgggg ttttgcaaag
     1801 attacgcgcg acgtgacaga acggcgcgcg cagcaggcag cgctcgaaga gagccggtca
     1861 gcattggcgc aggcacaaaa aatggaggca ttgggccagc tgacaggcgg gctcgcccat
     1921 gacttcaaca atctgctgag cgtgatcatc ggcggaatcg aatttgcgca gcggcactcc
     1981 gacacaggag attcgcaaag gagcgcgcaa atgctggatg cggcgaaaca ggccgcgcag
     2041 cagggcgctg cgctcgtccg ccgcatgctt gccttttcgc ggcgccagaa acttgccccc
     2101 cgggttgtcg acgtcaattc gctggttcga aacatgtccg agctga
//
LOCUS       DASZMC010000302         5943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3978597,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000302 DASZMC010000000
VERSION     DASZMC010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3978597"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1390)
                     /locus_tag="VG274_06215"
     CDS             complement(176..1390)
                     /locus_tag="VG274_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HWE06283.1"
                     /translation="MNTRQQWISRARIGAARAALALMVVPVLGLDGARPAQARGLTVL
                     YNFAGSSDGGDPFAGLIRDSAGNLYGTADYGGTAFAGAVFKVAPDGTETVLYSFSGGA
                     DGAQPFSALVRDKAGNLYGTTTMGGSANAGVVFKLDPGGTETVLHNFIGGTDGTTPTG
                     GLLEDKGGNFYGTASQGGTSNAGVLFKIGAKGKYSILHTFTGAANDGKYPTYTSLLMD
                     SVGALYGVTEEGGAANGGILYKFSKTGKLTILHSFMGGTTDGCNVLGTPFIDGNGTFY
                     GTTSSCGASSLGTVWMVSANGNETVLHSFAGGTTDGEYPLAGVIVDASGNIYGNTETG
                     GASNFGTVYGISPNGTFALLHSFDGADGKFPYGSFVRTARGTLFGTTQNGGTIGYGTV
                     WKMRKVSARRGG"
     gene            complement(1632..2498)
                     /locus_tag="VG274_06220"
     CDS             complement(1632..2498)
                     /locus_tag="VG274_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06284.1"
                     /translation="MDENGSPGDKNKPKEKESGFSVLINTPAGVTLLGFFLTGVLGAL
                     VSFSVNVFQRWDQERTTTLAERENEISSVNKQFVGAIVQRTFYVDAVIEAIGQSKAGS
                     DLDAAWPRYESAYQAELAAVWQNHLNIEGHLEDDPAPTGENGWQPFWYYLDLVIQPRF
                     QEMDHCLRAVHGAYLSAAGPQGERMKAAQHILEGCETSYHWDHLGKSQIASVALWDHF
                     ETCLESFTYHLDVSARIERQVWKAGETSVSLPDPGRCDRGDPICLQAVFLVGLKDSLA
                     EDCGPLPAGHYD"
     gene            2664..3065
                     /locus_tag="VG274_06225"
     CDS             2664..3065
                     /locus_tag="VG274_06225"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWE06285.1"
                     /translation="MTSRVASCSCGQLRLTCEGAPVRISICHCLECQKRTGSVFGTQA
                     RFSREFVTVDGRATQWTRLGDSGQPATFNSCPVCASTVYWEATGVAGFVTVAVGAFAD
                     PNFPPPGVSVYEERQHQWALTAGQLPLEHMA"
     gene            3272..4513
                     /locus_tag="VG274_06230"
     CDS             3272..4513
                     /locus_tag="VG274_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWE06286.1"
                     /translation="MSETPLPNIFDLTPLNPSFNENPHTLLDRLRTECPVHRDTHAGS
                     FVLTRYADVRGVLSDTTLWRDPDKAEPEAVLQKRIREERIEGIEMEQDEGRKGILLLD
                     DPDHARIRNPLAKALYKRVARCRPQVERVVDEILGRIDGRAQFDVMADFAILVPIDVI
                     ARILGVDDTRLAEFREWSEGVILTLNPFRTPDQTSRLVAAAIALSEYMKELIAARRAE
                     PRDDLVSDMVALQAEGAPVSDGELSINLQSLLVGGNLTTTDLIGNAVFHFLKNPGELE
                     KLKAEPALIKSAVEEVLRFDGPVDVTGRIASRDIEIGGCPIRKTQSVMLSLRGANHDP
                     EIFPDPHRFDISRKSAPHVAFGGGTHLCIGSPLARLEAQVALPALFNRFPKLRLAKPD
                     APPRWRSLPFFRGLQDLLVDT"
     gene            complement(4700..5302)
                     /gene="recR"
                     /locus_tag="VG274_06235"
     CDS             complement(4700..5302)
                     /gene="recR"
                     /locus_tag="VG274_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019236762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWE06287.1"
                     /translation="MASLPAGPEIEKLVQLLAKLPGLGPRSARRAALVLLKKREALLE
                     PLGQAMREAAAAIRVCTTCGNLDTTDPCAVCRDPRRDRHLLCVVEDVADLWALERANV
                     FRGRYHVLGGALSALDGVTPERLNVAPLAARVANGIEEVILAMNATVEGQTTAHYLMD
                     ILAASKVKVTRLAHGVPVGGELDYLDEGTLSAAFRARREM"
     gene            complement(5314..>5943)
                     /locus_tag="VG274_06240"
     CDS             complement(5314..>5943)
                     /locus_tag="VG274_06240"
                     /inference="COORDINATES: protein motif:HMM:NF023780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06288.1"
                     /translation="AAEMALVRLAYAADLPPTDKLVRDMTERAVSPPTPAPASRSPRP
                     APNATTSRGGEAVRAQARPLAESDDLRRPASAEPVALHTLADIAALAGAKGASVLKVQ
                     IENHMHLVRLEAGQIEFRPGSSAPRTLAADLAQKLREWTGIRWVVTLAREGGEATLAG
                     QKKAARAEKLEMVAQEPLVRAVLDRFPGAEIVAVRERADRDIAPPTDDS"
BASE COUNT         1055 a         1983 c         1779 g         1126 t
ORIGIN      
        1 ccatatcctg aaatagggat cgcggtaggg acgcgtattg ctacgcgccc ctaccgcgat
       61 ccattttttc tgctacgcat tcttccttat gccggcggcc ccacctcgcg gctcgtgagc
      121 agatacggtg atggaccgct aatcttgtga ttccggcaac tgccggacgc tgcgactacc
      181 ctcctcggcg cgccgacacc ttgcgcatct tccagactgt tccgtagccg attgtgccgc
      241 cattctgggt tgtgccgaag agcgtgcccc tcgcggtgcg aacgaagctg ccatacggaa
      301 atttgccgtc ggccccgtcg aagctgtgca agagggcgaa ggttccattc ggactgatcc
      361 cgtacaccgt tccgaagttg gacgcgccgc ctgtctccgt gttcccgtag atattgccgc
      421 ttgcatccac gatcacgccg gccaatgggt actccccgtc ggtcgttccc ccggcaaagc
      481 tgtgcagcac tgtctcattc ccgttcgcgc tcaccatcca gaccgtgccc aggctcgagg
      541 cgccgcagga cgatgtcgtg ccgtaaaagg ttccgttccc gtcgatgaac ggagttccca
      601 gaacgttgca gccgtcggtc gttcccccca tgaagctgtg caggatggtc agctttcccg
      661 tcttgctgaa cttgtacagg atcccgccat tggcggctcc gccctcttcg gttacgccat
      721 agagggctcc aaccgaatcc atcagaaggc ttgtatacgt tggatacttc ccgtcgttcg
      781 ccgcccccgt gaaggtgtgc agaatgctgt atttcccctt tgcgcctatc ttgaacagca
      841 cgcccgcatt cgatgttccg ccttgcgagg cggtaccata gaaattgccc cccttgtcct
      901 ccagcaaacc tcctgtcggc gtagttccat ccgttcctcc gatgaaatta tgcaatacgg
      961 tttcggtgcc tccgggatcg agtttgaaca ccacgcccgc gttcgcactt ccgcccatcg
     1021 tcgttgtgcc gtagagattg ccggccttgt cgcgcaccag tgcggaaaaa ggttgtgcgc
     1081 catcggcgcc gccgctgaag ctgtacagca ccgtttctgt tccgtcgggc gcaactttga
     1141 aaacagctcc ggcaaacgcg gtcccaccgt aatcggcggt gccataaagg ttccccgcag
     1201 agtcacggat cagacccgcg aaaggatcgc ccccgtcgga cgatcccgcg aagttataaa
     1261 gcacagtcaa accgcgcgcc tgcgccggcc gggccccatc caggcccaac accgggacga
     1321 ccatcaacgc cagagcggca cgcgccgctc caatacgagc cctggaaatc cattgctgtc
     1381 ttgtgttcat tgccgcgatc tccattcctt cgcccacagg tcgtagaagc gaaaccggta
     1441 taagccggaa ttcggcgacg gcatactctt tattgaatct gcggatgaca gcgagattcg
     1501 agatggcggc ccggaaagcg gcgcgcgatc ggccggaaga ggctttccgc gatgcgcgac
     1561 attctcctgc ggcaataatc tgcgataccc gtgctgccga cgaattcgga tataagtcga
     1621 ccaatcgcac gtcaatcata atggcccgca ggcagcgggc cgcaatcctc ggcgaggctg
     1681 tccttgagcc cgacaagaaa caccgcctgc aggcagatcg gatcgcccct gtcgcagcgg
     1741 cccgggtcgg gtagcgaaac ggatgtttcg cccgccttcc acacctgccg ctcgatcctg
     1801 gcgctcacat ccagatggta ggtaaaactt tccaggcagg tctcgaaatg atcccagagc
     1861 gccacggagg ctatctgcga cttgcccaaa tgatcccagt ggtagctcgt ttcgcacccc
     1921 tcaagaatgt gttgcgccgc cttcatccgc tccccttgtg ggccggcggc tgacaaatat
     1981 gcgccgtgga cggcacgcag gcagtgatcc atttcctgga accgcggctg gatgacgaga
     2041 tcgaggtagt accagaaggg ttgccatccg ttctccccgg tgggagctgg atcgtcttcg
     2101 agatggccct cgatgttgag gtgattttgc cagacggcgg caagttccgc ctgataggcg
     2161 ctttcgtagc gcggccaggc ggcgtcgaga tcgcttccgg ctttcgactg gccgatggcc
     2221 tcgatcaccg cgtcgacgta aaacgtgcgt tgcacgatcg caccgacgaa ttgtttgttg
     2281 acgctgctga tttcgttttc gcgctcggcg agcgtcgtcg tccgttcctg gtcccagcgc
     2341 tggaacacgt tcacgctgaa ggaaacgagc gcgccgagca cgcctgtcag gaaaaatccc
     2401 aggagcgtca cgcctgccgg cgtgttgatg agcacggaaa atccggactc tttctctttc
     2461 ggcttgttct tgtcgcccgg ggagccgttc tcgtccatcc atcaggtctc cgtttgcggc
     2521 gcgcatccat tccggccgtc ccgcacgcat gaacgatagc tgactcattg cgcgacgcgc
     2581 catactccga gacgcgcagc accttgcgtg gcggcggcgc gtgccgaata gtcgcggacg
     2641 cagtcacgcg aggagcatgc gcaatgacca gccgggtagc ttcatgcagc tgcggacagc
     2701 ttcgcctcac atgcgaaggc gcgcctgttc gcatctccat ctgccattgc cttgaatgcc
     2761 agaaacgcac cggcagcgtt ttcgggacgc aggcgcgctt ttcgcgcgag tttgtgaccg
     2821 tcgatggccg cgcgacgcag tggacgcggc tcggcgatag cggccagcca gccactttca
     2881 attcctgtcc ggtgtgcgcc tcgacggttt actgggaggc caccggggtg gcaggtttcg
     2941 ttaccgtggc ggtgggcgcg tttgccgacc cgaactttcc tccgcctggc gtctccgtct
     3001 acgaagagcg gcaacaccaa tgggcgctca cggcagggca gctgccgctg gagcatatgg
     3061 cctagccgtc ttgcttcttt gagaatgcgt ttgccggtcg cgtaaagcgg cggatgcccg
     3121 tgcgcatcgg gagctttgag aatccggcga cgccatgacc accatttggc cgcgcggcgc
     3181 cgggcggccg gctcccgtgg ggacgaaatg acaatttgtc ataacactcc tgctgcgtta
     3241 cggtgcacgg gttttcgagg acgccgccct catgtccgag actccgcttc ccaatatttt
     3301 cgacctgacg ccgctcaatc cgtcgttcaa tgagaatccg cacaccctgc tcgaccgtct
     3361 gcgcacggaa tgtcccgtgc atcgcgacac gcacgccgga agcttcgtgc tgacgcgcta
     3421 tgcggatgtg cgcggcgtgc tttcggatac gacgctgtgg cgagatcccg acaaggcgga
     3481 gccggaggcg gtgctgcaga agcgcatccg cgaggagcgc atcgaaggca tcgagatgga
     3541 gcaggatgaa ggccgaaagg ggattctgct gctggacgat cccgatcacg cgcgcatccg
     3601 caatcctctc gccaaggctc tgtacaagcg cgtcgcgcgc tgccggccgc aggtcgagcg
     3661 tgtagtcgac gaaattctcg gccgtatcga cggccgcgcg caattcgacg tcatggccga
     3721 tttcgccatt ctcgtgccca tcgatgtcat cgcgcgcatc ctcggcgtgg acgacacgcg
     3781 gctcgccgaa ttccgcgaat ggtcggaggg cgtcatcctt accctcaatc ccttccgcac
     3841 gcccgaccag acgagccggc tcgtcgctgc cgccatcgcc ttgtccgagt acatgaagga
     3901 actcatcgcc gcgcgccgcg cggagccccg cgacgatctc gtcagcgaca tggtggcgct
     3961 gcaggcggaa ggcgcgccgg tcagcgatgg cgagctgtcc atcaatctgc aatcgctcct
     4021 cgtcggcggc aatctcacga caaccgacct catcggcaat gcggtcttcc attttctgaa
     4081 gaatcccggc gagctcgaga agctcaaagc tgagcctgcc ctcatcaaat cggcggtcga
     4141 ggaagtgctg cgcttcgatg ggcctgtcga cgtcaccgga cgcatcgcct cgcgcgacat
     4201 cgagattggc ggctgcccga tccgcaagac ccagtccgtc atgctctccc tgcgcggcgc
     4261 caatcacgat ccggaaatct ttcccgatcc gcaccgcttc gacatttccc gcaaaagcgc
     4321 accgcatgtc gctttcggtg gcggcaccca tctctgcatc ggctccccgc tggcacgtct
     4381 cgaagcgcag gtcgcgcttc ccgcgctgtt caaccgcttc ccgaagctca ggctggcgaa
     4441 gcccgacgcg ccgccgcgct ggcgttcgct gcccttcttc cgcggccttc aggacctgct
     4501 cgtcgacacc tgaaaatcac aaccgccccg aaccggtcac gggtgacaca gatttaacgc
     4561 agagtacgca gaggttgcgc cgcgggcgca agaaaaaaat gacagcgcgc gaagcgcgca
     4621 aaataacgct gcgtcctccg cgcaacctct gcgtactctg cgttaaaaat ctgcctgcac
     4681 gcgactctcg caggatcaac tacatttcgc ggcgggcgcg gaaggctgcg gagagcgtgc
     4741 cctcgtcgag ataatcgagt tctcccccga caggcacgcc gtgggcgagg cgcgtcacct
     4801 tcactttcga cgcggcaaga atgtccatca gataatgcgc ggtggtctga ccctccaccg
     4861 tcgcattcat cgcgaggatc acctcctcga taccattcgc cacgcgggcc gccagcggcg
     4921 cgacattcaa tctttcaggc gtgacaccgt cgagcgcgga caacgcgccg cccagaacgt
     4981 ggtagcgtcc gcggaagaca ttcgcacgtt cgagcgccca caggtcggcg acgtcttcca
     5041 ccacgcaaag caggtggcga tcgcggcgcg ggtcgcggca cacggcacac ggatctgtcg
     5101 tgtcgagatt gccgcaggtc gtgcagacac ggatcgcggc cgccgcttcg cgcatcgcct
     5161 ggccgagcgg ctcgagcagc gcctcgcgct tcttgagcag aaccagcgcc gcccggcggg
     5221 ccgaacgcgg accgagcccc ggcagcttcg ccagcagctg caccagcttt tcgatctcgg
     5281 gaccggcggg aagagaggcc atgaatcagg ggatcacgaa tcgtccgtag gcggagcgat
     5341 gtcgcgatcc gcacgctcgc gcaccgcgac gatttccgcg ccgggaaagc ggtcgagcac
     5401 cgcgcgcacc agaggctcct gcgccaccat ttcgagcttc tcggcgcgcg cagctttctt
     5461 ctgtccggcg agcgtggcct caccgccttc acgcgccagc gtcaccaccc agcgaattcc
     5521 ggtccattcc ctgagctttt gcgcaagatc ggcggccagc gtgcgcggcg cgctcgaccc
     5581 ggggcggaac tcgatctgcc ccgcttcaag gcgcacgaga tgcatgtgat tttcgatctg
     5641 caccttgagg acggaggcgc ccttcgcgcc ggcgagcgcc gcgatgtcgg ccagcgtgtg
     5701 cagcgctacg ggttccgcgg aagccggacg gcgcaagtcg tcgctttcag ccaaagggcg
     5761 cgcctgggcc ctcaccgcct cgccgccgcg ggaggtggtc gcatttggcg ccgggcgggg
     5821 ggagcggctc gccggcgcgg gcgtcggggg cgataccgcg cgctccgtca tgtcgcgcac
     5881 caatttgtcc gtaggcggaa gatcggccgc ataggccagc cggaccagcg ccatttcggc
     5941 cgc
//
LOCUS       DASZMC010000303         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1252610,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000303 DASZMC010000000
VERSION     DASZMC010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252610"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2698)
                     /locus_tag="VG274_06245"
     CDS             complement(<1..2698)
                     /locus_tag="VG274_06245"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWE06289.1"
                     /translation="MQISPDAAQPVARRLAALADAARTMAMAMEFGFLFDSDRQLLSI
                     GYRDNDGSLDSNFYDLLASEARLASFFAIAKGDVPAKHWFALGRTLTPIDGGSGLISW
                     SGSMFEYLMPSLVMRAPSGSLLEQTTRLAVWRQKKYGEELGVPWGISESEYYARDMEQ
                     TYQYSSFGVPDLGYKRGLGESTVIAPYASGLASMIDPAAAAKNYSRMAGIGARGVYGW
                     YEAIDYTRARLPDGAKFALVRAYMAHHQAMTIVGIANALHDGRMRARFHAEPIVQATE
                     LLLQERMPRDIAMARPAPEKATAVTQIASLIPEVQRRYDSAHSRVPRTHLLSNGRYSA
                     MLTAAGSGYSRWHDIAITRWREDVTRDGWGAYIFLRDVRSGETWSAGYQPSLVEPDAY
                     EVTFYEDRAEILRSDGTISTMLEVAISPEEDAEVRRVSITNHGIRAREIDLTSYAEVA
                     LARQADDTAHPAFSKLFVETEFVSGLGAILAARRQRSAGDPKVWAAHLSVVEGESAGD
                     VQFETDRVRFLGRGQTIQNSAAISEGWPLSNTAGTVLDPIFSLRRRVKIPRGGTARVS
                     FWTVAAASRDEVIDLADKHHDATAFGRATTLAWTQSQMQLLHLGIGTDEAHLFQRLAN
                     HVLYSDPTLRPPSDVLRRGARKASTLWSQGLSGDLPIVLVRIEENDELELVRQLLRAH
                     EYWRLKQLPVDLVILNERAASYVQDLQIDLDTLVRMNQSMPRLASADAQGAVFVLRAD
                     LIPVEVRNLLSSSARAVLQGSRGSLAEQVNRAREMKPGSAPPPRLAVSAQVPETPLPR
                     PQMEFFNGMGGFTNDGREYLTVLTGADRTPAPWINVIANSSFGFQVSTDGSGFTWAVN
                     SQQNQLTPWSNDPVGDAPGEVIYVRDEDSGEVWGPTALPIREK"
BASE COUNT          510 a          833 c          863 g          506 t
ORIGIN      
        1 tcttctcgcg aatcggcagc gccgtcggtc cccagacctc gcctgaatcc tcgtcgcgca
       61 catagatgac ttcgccaggc gcgtcgccaa cgggatcgtt agaccagggc gtgagctggt
      121 tttgctggct gttcacggcc caggtgaatc cgcttccgtc ggttgagacc tgaaatccga
      181 acgacgaatt ggcgatgaca ttgatccagg gcgccggcgt ccggtccgcg ccggtcagca
      241 ccgtcagata ttcccgcccg tcattggtga agccgcccat cccgttgaag aattccatct
      301 gcggccgggg caatggcgtt tcgggcacct gtgccgatac cgccagccgc ggcggcggcg
      361 cgctgcccgg cttcatttcg cgggcacggt tcacctgctc ggccaacgaa ccgcggctgc
      421 cttgaagcac ggcgcgggcg ctggaagaca gcagattgcg gacctccaca gggatcaggt
      481 cggcacgcaa gacaaatacc gcgccctgcg cgtcggcgct cgcgagtcgc ggcatcgact
      541 gattcatccg taccagcgtg tcgaggtcga tctgcaaatc ctggacataa gatgctgcgc
      601 gctcgttcag gattaccaga tcgacgggaa gctgtttcag ccgccaatat tcatgggcgc
      661 gcagaagctg gcggacgagc tcgagctcat cgttttcttc gatgcggaca agaacgattg
      721 gaagatcgcc ggacagtccc tgcgaccaga gcgtggacgc tttgcgcgcg ccgcgcctga
      781 ggacatcgga cggcggtctc aatgtagggt cggaataaag cacgtgattg gcaagacgct
      841 ggaaaagatg agcttcgtcg gtgccgatgc cgagatggag caactgcatc tgcgactggg
      901 tccatgcgag cgtggtggcg cgcccgaacg ccgtcgcgtc atgatgcttg tccgcgagat
      961 cgatcacttc gtcgcgcgag gcggcggcca ccgtccagaa cgagacgcgc gccgtcccgc
     1021 cgcgcgggat tttgacgcgc cgccggaggc tgaaaatcgg atcgagcacc gtgcctgccg
     1081 tattggaaag cggccagccc tcggagatgg cggcggagtt ctgtatcgtt tgtccccggc
     1141 cgaggaaccg tacgcgatcg gtttcgaact gaacatcgcc cgcgctctcg ccctcgacga
     1201 cggaaagatg ggcggcccac actttcggat cgccggccga gcgctgccgc cgcgctgcca
     1261 gtattgcgcc cagaccgctc acgaactccg tttccacgaa cagcttggag aaggcagggt
     1321 gggccgtatc gtccgcctga cgcgccagtg ctacttcggc gtaggaggta agatcgattt
     1381 cgcgcgcgcg gattccgtga ttggtgatcg acacgcggcg aacttcggca tcttcctccg
     1441 gcgaaatcgc aacttccagc atggtcgata ttgtcccgtc gctgcggaga atctcggcgc
     1501 ggtcttcgta gaacgtgact tcgtaggcgt cgggttccac gaggctcggc tggtatccgg
     1561 cggaccaggt ttcaccgcta cgcacgtcgc gcaaaaagat atacgcgccc caaccgtcac
     1621 gggtaacgtc ttcgcgccag cgcgtgattg caatgtcgtg ccagcggcta tatccggatc
     1681 ccgctgccgt cagcatcgcc gaataacgtc cgttcgacag cagatgcgtg cgcggtacgc
     1741 gcgaatgtgc agaatcatag cggcgctgaa cctctggaat gaggctggcg atctgcgtga
     1801 cggcggtcgc cttttcgggg gccggccgcg ccatggcaat gtcgcgcggc atgcgttcct
     1861 gcagcaacag ctccgtagcc tggacgatgg gttcggcgtg aaatcgcgcc cgcatgcgcc
     1921 cgtcatgaag cgcattcgca atgccgacga ttgtcattgc ctggtgatgc gccatatagg
     1981 cgcggacgag ggcaaatttg gcgccgtcgg gaagccgcgc gcgcgtgtag tcgatcgctt
     2041 cgtaccagcc gtagacgcct cgtgcgccga tacccgccat gcgactgtag ttttttgccg
     2101 cggccgcggg atcgatcatg gacgccagcc cgctggcgta gggcgcaatg accgtgcttt
     2161 cgccgaggcc ccgcttgtac ccaaggtcgg gcacgccgaa gctggaatat tgataagtct
     2221 gctccatgtc gcgtgcgtag tattccgatt ccgatatgcc ccatggcacg ccgagttcct
     2281 cgccgtattt cttctggcgc cacacggcga gccgggtagt ctgttccaga aggcttcctg
     2341 acggcgcgcg catcaccagc gacggcatca gatattcgaa catcgatccg gaccacgaga
     2401 tcaatccgga accgccatcg atcggcgtca gggtgcggcc aagagcaaac caatgcttgg
     2461 ccggaacatc gcctttggcg atggcaaaga aacttgcgag acgggcttcc gacgcaagca
     2521 gatcatagaa gttggaatcg agggagccat cattgtcgcg atagccgatg gaaagaagct
     2581 ggcggtcgga atcgaacagg aaaccgaatt ccatcgccat cgccatcgtc ctggcggcat
     2641 cggccaaagc cgcgagccgc ctggcaacag gttgggcggc gtctggggat atttgcaatg
     2701 caggcttcgg cg
//
LOCUS       DASZMC010000304         8165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1253448,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000304 DASZMC010000000
VERSION     DASZMC010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253448"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VG274_06250"
     CDS             complement(<1..930)
                     /locus_tag="VG274_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWE06290.1"
                     /translation="MTQRDAGALIAPAMKEPPEMPLSNAQTRDVESVLHPYTNLLKLR
                     ETGPLVIERGEGVRVYDDRGKDYIEALAGLWCTALGWGEEALVEAAAGQMRKLAFGHL
                     FAGKSHEPAIALAEKLKEIAPFPVGKVFFANSGSEANDTQIKLFWYAANARGTPARKT
                     IISRTKAYHGVTIAAASLTSLPANHKSFDLPLDFARFAECPHHYRAAEPDESERDFSS
                     RLAHNLDALIEKEGADTIAAMIVEPVQGAGGVILPPEGYFAAIGGVLERHGIPLIDDE
                     VITGFGRTGNWFGCATYGFQPASMTLAKAMSSAY"
     gene            1006..2628
                     /locus_tag="VG274_06255"
     CDS             1006..2628
                     /locus_tag="VG274_06255"
                     /inference="COORDINATES: protein motif:HMM:NF004674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HWE06291.1"
                     /translation="MFGLMQDWPLLVHKIIDHAAVYHGDREVVTRSVEGPIHRTNYRE
                     IRRRAARAASALEKIGVNAGDRVATLGSNTWRHLESWYGIAGMGGVYHTLNPRLFADQ
                     IVYIANHAEDRVLFFDPTCTALVGEIAPRLKTVTHFIVFADRAPQTSFGPVVAYEDFI
                     ATGDENYIWKEFDEHAACGLCYTSGTTGNPKGVLYSHRSNVLHAFMACQPDAMGMRNA
                     DTVLPVAPMYHANAWAIAFLAPMTGAKLVMPGPKLDGASVYELLDSEKVTLTAAVPTV
                     WLSLLHYLEETGKVLPHLKRVLIGGSACPRTIIETFEKKFGVDVVHAWGMTEMSPLGT
                     LGSLTAAVAELSENERIAHKLKQGHPLFGVEMKIVDGSDRELPRDGTAFGRLKVRGPA
                     VAKGYFRGEGEDAFDRDGWFDTGDVATLGADGYMTITDRAKDVIKSGGEWISSIEIEN
                     IAVGHKAVAEAAVIGISHPKWDERPLLIVLLKPGQTATKAEMLQFLEGKIAKWWMPDD
                     VVFANEIPHTATGKIQKTVLRERYGRHRPLPG"
     gene            2670..3443
                     /locus_tag="VG274_06260"
     CDS             2670..3443
                     /locus_tag="VG274_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HWE06292.1"
                     /translation="MTTTARLSFAALIVSAAIGLAAGFGTRIHLWDYQVGLLRIFPYC
                     LYAGLAAFALGFLWALCAVVSASAAGVRFGVIGLAGSIALLWIPLRDIYLVSIDHSIP
                     PIHDISTDTEHAPEFVALRNNRPGAINPPDYDGPRPVLFLGRIYTTEALQKLYYGDIK
                     SASILGITPAKLFQHALAAAQEMGWNIVAVAPDSSGGRIEATDATLLFGLTSDIVIRV
                     RPAGIGARLDIRSKSRVGASDFGRNARRIRAYLKKLAGS"
     gene            complement(3516..4400)
                     /locus_tag="VG274_06265"
     CDS             complement(3516..4400)
                     /locus_tag="VG274_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWE06293.1"
                     /translation="MPIPFDRTFDAPYGECVKIGERMHRVLAPNPSPFTFKGTGVYIL
                     GARDVAVIDPGPNIPIHIEALKRALAGKNVSHILLTHAHNDHSPAAAPLKEWTGAKTF
                     AYGPHGSGGSDNETKVEEGGDRTFMPDVRIRDGDVLEGDGYTIECVFTPGHTSNHMCY
                     ALREEKALFTGDHVMGWSTTVVAPPDGDMAAYRASLRKLLARDDRILWPTHGGPIRDP
                     GPFLQAYLDHRAEREAQIIACLRENVQHIPDIVARLYADVDKRLHPAAARSVLAHLIE
                     LEYEGRVTRDGDAYRMSR"
     gene            complement(4409..4744)
                     /locus_tag="VG274_06270"
     CDS             complement(4409..4744)
                     /locus_tag="VG274_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06294.1"
                     /translation="MTRIAMKKFLILTGGWVVTIVGLLLTPIPLPLPFPLGIMVFLLG
                     CAILTAHSKAFRRLVQQLRHRNGWLSRGLEFLTRRAPRQVKTLEAATRPLALGRQARM
                     RARHDGETV"
     gene            4849..5460
                     /locus_tag="VG274_06275"
     CDS             4849..5460
                     /locus_tag="VG274_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWE06295.1"
                     /translation="MKFYNSIGPNPRVVRMFLAEKALRIPVVQIDLLKGENRCEPYLQ
                     VNPHGQMPALELDDGSSICEITAICEYLEGIHPAPVLIGANPRDKAECRMWTRRVDLN
                     ICEPLANGFRFGEGLGLFRDRIVTVPEASDGLKRMAGDRLGWLDRQMQAREFIGGTRF
                     TLADILLFCFLDFAAGVGQRLDPANANLTAWFGRIKARPSTSA"
     gene            5551..6228
                     /locus_tag="VG274_06280"
     CDS             5551..6228
                     /locus_tag="VG274_06280"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HWE06296.1"
                     /translation="MRRISGILASLAIVAAICTIFAAGAPARADQMSLLSSSEATVDN
                     MKRDATFGPSRDLLRRARAVLIVPQLVKGGFIFGGEGGDGVMLARTRRGWSSPAFYTI
                     GSASFGLQIGLESAQLVMFVMSDRALHALEQSKFKFGGEAGLTVVTLGANAQGATSTN
                     LTGDIVIWASSKGAYGGLTLEGSVLAPKDDWNSRFYGRPVSVPQILSNEAGNAAATPL
                     RRDLTSL"
     gene            complement(6272..6610)
                     /locus_tag="VG274_06285"
     CDS             complement(6272..6610)
                     /locus_tag="VG274_06285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWE06297.1"
                     /translation="MLVCDPGDVAIVPFPFTDIAFSKPRPALALSARKANEESGNTIF
                     AMITTAARSPWAQDVPISDDQAAGLTAASIVRVKLFTLDNRLVSRKIGALSLRDRQFV
                     RRMLRGLLAV"
     gene            complement(6610..6840)
                     /locus_tag="VG274_06290"
     CDS             complement(6610..6840)
                     /locus_tag="VG274_06290"
                     /inference="COORDINATES: protein motif:HMM:NF027267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HWE06298.1"
                     /translation="MSVLFSKVTAKAQTTVPKQVREALGVKAGDMLVYRIAKGKVTLA
                     RAEPLDRAYLKAVESTLSEWSSAEDAAAYDGL"
     gene            7154..>8165
                     /locus_tag="VG274_06295"
     CDS             7154..>8165
                     /locus_tag="VG274_06295"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWE06299.1"
                     /translation="MGAQPNSGERDQVLLGVTGSGKTFTMAKVIEALQRPALILAPNK
                     TLAAQLFAEMKSFFPENAVEYFVSYYDYYQPEAYIPRTDTYIEKDSSINEEIDRMRHS
                     ATRAILERDDVIIVASVSCIYGIGSVETYSGTAVGIARGDKVDRNAAIQNLVALQYRR
                     NDDNFTRGAFRVRGDTIDIFPAHYEDRAWRVELFGDVVDSITEFDPLTGHKAGVLDAI
                     KIYANSHYVTPRPTLQQAMKGIKAELIATLADFRAKGKLLEAQRLEQRCTFDLEMIEA
                     TGSCAGIENYSRWLTGRKPGEPPPTLFEYLPDNALVFCDESHVSVPQIGAMYKGDYRR
                     KFT"
BASE COUNT         1518 a         2534 c         2564 g         1549 t
ORIGIN      
        1 ataggccgaa gacatcgcct tggcgagcgt catgctggcc ggttggaacc catacgtcgc
       61 gcagccgaac cagttgccgg tgcgcccgaa tccggtaatc acctcatcgt cgatgagcgg
      121 aatcccgtgc cgttccagca caccgccgat cgcggcgaaa tatccttcgg gtggaaggat
      181 cacgccaccg gcgccttgca cgggctccac gatcatcgct gcgatcgtat cggcgccctc
      241 cttttcgatc agcgcgtcga gattgtgcgc aaggcgcgaa gagaagtccc gttcgctctc
      301 gtcgggctcc gccgcgcggt agtggtgcgg gcattccgcg aagcgcgcga agtcgagcgg
      361 cagatcgaag ctcttgtggt tggccggcag gctggtcagg ctcgccgcgg caatcgtgac
      421 gccgtgatag gctttcgtgc ggctgatgat ggttttccgc gccggcgttc cccgcgcatt
      481 tgcggcatac cagaacagct tgatctgggt gtcgttcgct tccgagccgg aattggcgaa
      541 gaacaccttg cccaccggaa acggtgcgat ctccttcagc ttctctgcaa gcgcgatggc
      601 cggctcgtgg ctcttgcccg caaagagatg cccgaaggcg agcttgcgca tttgtccggc
      661 agcggcttcc acgagcgctt cctcgcccca cccgagcgcg gtgcaccaca gccccgccag
      721 tgcctctatg tagtccttgc cccgatcgtc atagacccgc accccctcgc cgcgttcgat
      781 gacgagcgga ccggtttcgc gaagtttcag cagattcgtg tagggatgaa gaacactttc
      841 cacatcgcgg gtctgggcgt tgctgagggg catttccggc ggttccttca ttgccggcgc
      901 gattaacgcg ccggcgtcgc gctgcgtcaa cgcgcgggcc gcacctcgtt gcccggatcc
      961 attctcgttc ctatgatggc cgaaggtaaa aaggggctac ccgcaatgtt cggcctgatg
     1021 caggactggc cgctcctggt acacaagatc atcgatcacg cggctgtcta tcacggggac
     1081 cgcgaggtcg tgacgcgcag cgtcgaaggg ccgatccatc gcaccaatta tcgcgagatt
     1141 cgcaggcgcg ccgcccgcgc agcgagtgcc ctcgaaaaga tcggcgtgaa cgccggtgat
     1201 cgggtggcga cgctcgggtc gaatacctgg aggcatctcg aatcctggta cgggattgcc
     1261 gggatgggcg gcgtctacca cacgctcaat ccacgcctgt tcgccgatca gatcgtctat
     1321 atcgccaatc atgcggagga ccgggttctc tttttcgatc cgacatgcac tgcgctggtc
     1381 ggcgagatcg cgccacggct caagacggtg acgcatttca tcgtgttcgc agatcgggcg
     1441 ccccagacca gtttcggtcc tgtcgtcgcg tacgaggact tcatcgcgac gggcgacgaa
     1501 aactacatct ggaaggagtt cgacgagcac gcggcctgcg gcctctgcta cacgtcgggc
     1561 acaaccggaa atccaaaagg cgttctctac agccatcgct cgaatgtgct gcacgccttc
     1621 atggcatgcc agcccgatgc gatggggatg cgcaatgcgg atacggtgct gccggtcgct
     1681 ccgatgtatc acgccaatgc gtgggcgatc gccttcctgg ccccgatgac cggcgccaag
     1741 ctcgtcatgc cgggcccgaa gctcgacggc gcgtcggtct acgagcttct cgacagcgaa
     1801 aaggtgaccc tgacggccgc cgtgccgacg gtgtggctct cgctgctcca ttatctggaa
     1861 gagaccggaa aggtgttgcc gcacctcaaa cgcgtgctga tcgggggctc ggcctgtccg
     1921 cgcaccatca tcgaaacttt tgagaagaaa ttcggcgtcg atgtcgtgca tgcgtgggga
     1981 atgaccgaga tgagtccgct cggcacgctc ggttcgctga ccgcggccgt ggccgaactc
     2041 tccgaaaacg aacgaatcgc ccacaagctc aagcagggac acccgctctt cggagtcgag
     2101 atgaagatcg tcgacggttc cgaccgggaa ttgccccgcg acggcacggc gttcgggaga
     2161 ttgaaggtgc gcggccccgc cgtcgcgaag ggctacttcc gcggcgaggg agaagacgca
     2221 ttcgacaggg acggctggtt cgatacgggc gacgtggcga cgctgggcgc cgacggctat
     2281 atgacgatca ccgaccgggc caaggacgtg atcaaatccg gcggcgaatg gatttcctcg
     2341 atcgagatcg agaacatcgc cgtgggccat aaggcggtgg cggaagccgc cgtgatcgga
     2401 atttctcacc cgaaatggga cgaacggcct ctgctgatcg tcttgctgaa gcccggtcag
     2461 accgccacca aggccgagat gctgcaattc ctcgagggca agattgcgaa gtggtggatg
     2521 cccgacgacg tcgtctttgc aaatgaaatt ccgcacactg cgacaggaaa aatccagaag
     2581 accgtgctgc gcgagcgcta tggtcggcat cgcccgcttc ccggttgatt ccgggcggcg
     2641 cggctccgag gggggaagcg atgcggttca tgacgactac ggcgaggctg tcgttcgccg
     2701 cgctgattgt ctccgccgca atcggattgg ccgcggggtt cggaacgcgc attcatcttt
     2761 gggactatca ggtcggtttg ctgagaatat tcccgtattg cctttacgcc ggactggcag
     2821 ctttcgcgct cggcttcctg tgggcgctgt gtgccgtcgt atcggcttcg gccgccggcg
     2881 tgcgattcgg cgtaatcgga ctggccggtt cgattgcgct cctgtggatt ccgctgcgcg
     2941 atatctatct tgtgagcatc gaccattcga ttccgcccat tcacgacatt tcgacggata
     3001 ccgagcacgc gcccgaattt gtggcgctgc gcaacaatcg tccgggagca atcaatccgc
     3061 ccgattatga cggaccgagg cccgtgttat tcctgggaag gatttacacg acagaggcgc
     3121 tgcaaaagct ttattatggc gacataaaat ccgcctcgat tctcggaatc acgccggcaa
     3181 agctgtttca acatgctctc gcggccgcgc aagaaatggg ctggaacatc gtggccgtcg
     3241 cgccggactc ctccggcggc cgcatcgagg ccacggacgc gacgctgctg tttggtctta
     3301 cctcagacat tgtcatccgc gtcaggccgg ccggaatcgg agcaagactc gacattcgct
     3361 ccaagagccg cgtcggtgct tcggacttcg gcaggaacgc cagacgcatc cgcgcctatc
     3421 tgaagaagct tgcgggaagt tgacccttcg acaagctcag ggtgaggact taattgaaat
     3481 ccctcatccc gagcctgtcg aaggatgaac acactctatc gcgacattct gtaagcgtcg
     3541 ccgtcgcgcg tgacgcggcc ctcgtattcc agctcgatca aatgggccag tacggagcgc
     3601 gcggcagctg gatggagtcg tttgtccaca tccgcgtaca ggcgcgcgac gatatcggga
     3661 atgtgctgca cgttttcgcg caggcaggca ataatctgcg cttcgcgttc ggcgcgatga
     3721 tcgagatagg cttgcaggaa tggcccggga tcgcggatgg ggccgccatg cgtcggccag
     3781 aggatgcggt cgtcgcgcgc cagcagtttc ctgaggctcg cgcgataggc cgccatgtcg
     3841 ccgtcggggg gcgcgacgac ggtcgtcgac cagcccatca cgtgatcgcc ggtgaagagc
     3901 gccttctctt ccctgagcgc gtagcacatg tgattggacg tgtgtcccgg cgtgaaaacg
     3961 cattcgatcg tgtagccgtc tccctcgagg acgtcgccgt ccctgattcg cacgtcgggc
     4021 atgaatgtgc ggtcgccgcc ctcctcgacc tttgtttcat tgtcgctgcc gcccgagccg
     4081 tgcggtccat aggcaaaggt tttcgcgccg gtccattcct tgagaggcgc ggccgcaggc
     4141 gagtgatcgt tgtgcgcgtg cgtcagaagg atatgcgata cgtttttgcc ggcgagggct
     4201 cgcttgagcg cctcgatgtg tatgggaatg ttcggccccg gatctatcac cgcgacgtcc
     4261 cgcgcaccga ggatgtagac acccgtgccc ttgaacgtga acggacttgg gttgggcgca
     4321 agcacacgat gcatccgctc gccgatcttt acgcattccc catacggcgc gtcgaaggtg
     4381 cggtcgaatg gaatgggcac gacagacgtc aaaccgtctc gccatcgtgg cgtgcgcgca
     4441 tgcgcgcctg gcgtccgagc gccaggggcc gcgttgctgc ttccaaagtt ttcacctgtc
     4501 tgggcgcgcg ccgggtcaaa aactcgaggc ctcgcgacag ccacccattg cgatgccgca
     4561 attgctgcac gaggcgccga aatgccttgg aatgcgccgt caatatcgcg cagcccagga
     4621 ggaacaccat gatgccgaga ggaaacggaa ggggcaaagg aatgggcgtg agcaggaggc
     4681 ccacgatggt gacaacccat cctcccgtca gaatcagaaa cttcttcatc gcgatccggg
     4741 tcatgccgtc ggcaagctgg gtgcggacgt accggattgc aatggcaaat tgaccctgcg
     4801 gtgtggggtg tctagcattt ccgaaccgtt ccggccggag ttcgaataat gaaattctac
     4861 aattccattg gtccaaaccc gcgcgtcgtg cggatgttcc tggcagaaaa agctttacga
     4921 attcccgtcg tgcagatcga tcttttgaag ggcgaaaacc ggtgcgagcc ctaccttcag
     4981 gtcaacccgc acggccagat gccggcattg gagctcgatg acggttcttc gatctgcgaa
     5041 atcactgcga tttgcgagta tctggagggc attcaccccg cgccggtcct catcggcgcc
     5101 aatccccgcg acaaagccga gtgccgcatg tggacgcgcc gggtcgatct gaacatctgc
     5161 gagccgctcg ccaatggatt ccggtttggc gaaggtctgg gtctgttcag ggatcgcatc
     5221 gtcacggttc cggaagcgtc cgacgggctc aagcgcatgg ctggagaccg tctcggctgg
     5281 ctggatcgtc agatgcaagc ccgcgaattc atcggcggca cgcgatttac cctggccgac
     5341 attctgctgt tctgctttct cgattttgcg gccggcgtcg gccagaggct ggatccggcg
     5401 aacgcgaacc tgacggcctg gttcggtcgc ataaaggcgc gaccctccac gtcggcctga
     5461 ctttagcaaa aggcaacccg cctgcccctg cgcgcgttat ctctacgcgc gaacgcagtg
     5521 ttgcgcgcgc atcttgagga ggaaacttcc atgcgacgaa tctcgggaat tctcgcgtcg
     5581 ctcgccattg ttgccgcgat atgcacgatc ttcgcggccg gcgcgcccgc gcgagccgat
     5641 caaatgtcgc ttctgtccag ctccgaggcg acggtcgaca acatgaaacg ggacgcgacc
     5701 tttggaccgt cgcgcgacct cctgcgccgc gctcgcgcgg tgctgatcgt gccacaactc
     5761 gtcaagggcg gattcatctt cggcggcgag ggcggagacg gcgtgatgct ggcgagaacg
     5821 cggcgaggct ggagttcgcc ggcattctac acgatcggtt cggcttcctt cggcctgcag
     5881 atcggtcttg aatcggccca actcgtcatg ttcgtcatga gcgaccgcgc gctgcatgcg
     5941 ctcgagcaga gcaagttcaa attcggcggc gaagccggac tgaccgtcgt gacgctcggc
     6001 gcaaatgcgc aaggcgccac cagcaccaac ctcacgggcg acattgtcat ctgggcttcg
     6061 tccaagggcg cctatggcgg gctgacgctg gagggttcgg tccttgcccc caaagacgac
     6121 tggaattcgc gattctacgg gcgcccggtg agcgtgccgc agattctcag caatgaggcc
     6181 gggaatgcgg ccgcgacgcc cctgaggcgc gatctgacga gcctctgaga cgaccccggg
     6241 ttgatcgcgg tgtgttcgtg gcctgggccc gtcacaccgc gagcaatccg cgcagcatcc
     6301 gacgaacaaa ttggcgatct cgcaaggaca gcgcaccgat tttccgcgaa accagccggt
     6361 tgtcgagcgt gaacagcttg acgcgaacga tggaagcagc ggtcagcccc gcagcctgat
     6421 catcggaaat cggcacgtcc tgagcccacg ggctgcgcgc ggcggtggtg atcatcgcaa
     6481 agatcgtgtt gccggactcc tcgttggctt tgcgcgctga aagcgccagg gcggggcgcg
     6541 gtttcgaaaa cgcaatgtcc gtgaacggga acggcacgat ggccacgtcg cccggatcgc
     6601 agaccagcat cagaggccgt catacgccgc ggcgtcctcg gcgctcgacc attcggaaag
     6661 tgtcgactcg accgctttga gataggcgcg atcgagaggt tcggcgcggg ccaacgttac
     6721 ctttcccttg gcgatgcgat agacgagcat gtcgccggct ttcaccccca aagcctcgcg
     6781 cacctgcttt ggaacggtgg tctgcgcctt ggccgtcacc ttgctgaaaa gcacgctcac
     6841 atccttgcct ccttgctcct tacggtaagg acacagcggc tggcgggcaa gcgcggggac
     6901 gcctatattc ggatttatgg cgcacaacaa cggcatgatt ccgcgcgggg tctccgagtc
     6961 ccaggcacag tttcgtatcg ccggcggcgc agtgcctccg gcgatgccga tgacggccgc
     7021 ggaggtcgcg gctttcgtgc cgcatcgccc cgcgcgtccg gagaaatccg aaggcggcag
     7081 gcggctgcgc ctggtcagtg gatacgagcc tgcgggcgac cagccgacgg ccattgcaga
     7141 tctcgtggaa ggattgggcg cgcagcccaa cagcggcgag cgcgaccagg tgctgctggg
     7201 cgtcaccggt tctggcaaga ccttcaccat ggcgaaggtc atcgaggcgc tgcagcgccc
     7261 cgcgctcatc ctcgcgccca acaagaccct ggccgcgcaa ttgttcgctg agatgaagag
     7321 cttctttccc gagaatgcag tcgaatattt cgtgtcctac tacgactact atcagccgga
     7381 agcctacatt ccgcgcaccg acacctacat cgagaaggat tcgtcgatca acgaagagat
     7441 cgatcgcatg cgccattcgg cgacgcgggc gatcctcgaa cgcgacgacg tgatcatcgt
     7501 cgcctccgtg tcctgcatct acggcatcgg ctcggtggag acctattccg gcaccgccgt
     7561 cggcatcgcc cgcggcgaca aggtcgaccg caatgccgcg atccagaatc tggtggcgct
     7621 gcaatatcgc cgcaacgacg acaatttcac ccgcggcgct ttccgggtgc ggggcgacac
     7681 catcgatatt ttcccggctc attatgaaga ccgcgcctgg cgggtggagc tgttcggcga
     7741 tgtggtggat agcatcaccg aattcgatcc cttgaccggc cacaaggcgg gcgtgctgga
     7801 cgcgatcaag atttacgcca acagccatta cgtcacgccg cgcccgaccc tgcagcaggc
     7861 gatgaaaggc atcaaggccg agctgatcgc caccctggcc gacttccgcg ccaagggaaa
     7921 attgctggaa gcccagcggt tggaacagcg ctgcaccttc gacctggaga tgatcgaggc
     7981 cacgggatcg tgcgccggga tcgagaatta ttcgcgctgg ctgacgggcc gcaagcccgg
     8041 cgagccgccg cccaccctgt tcgaatacct gcccgacaat gccctggtgt tctgcgacga
     8101 aagccatgtg tcggtgccgc agatcggcgc catgtacaaa ggcgactatc gccgcaagtt
     8161 cacgc
//
LOCUS       DASZMC010000305         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2600514,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000305 DASZMC010000000
VERSION     DASZMC010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600514"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VG274_06300"
     CDS             <1..680
                     /locus_tag="VG274_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06300.1"
                     /translation="DLEVPGELPETAAAWRAQQARWTKGHAQVARKLLPRILSSRMPL
                     WKKLVMSFQVCQFAFYTLAAVSAVISIALMSMGVTYLQSVAELGIAVTIIGVGTSVGY
                     LYCGQLMLGRAGEPLLPQSLLLAIVFPSGLILSNARATFEAFWRSDLVFVRTPRAGEF
                     AVGGWRGRPEIAAGLLLPVFALAEQAWSAPFFVFAAAGLLSVGAMGWNGTGEVPAREA
                     LRLKSQN"
     gene            833..1354
                     /locus_tag="VG274_06305"
     CDS             833..1354
                     /locus_tag="VG274_06305"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HWE06301.1"
                     /translation="MLALGAAGCASAPDDDFGSGDLRGLAPSTEGASVETPSTPVECV
                     PYARARSGISLYGDAGTWWGKADGRYTRSHTPLLGSVIVLTGYAEPGRGHVGVVSALV
                     SSREIRLDHANWLGDGAIYRDDPVADVSPDNDWTEVRVWNTRLHAWGLRTYSVEGFIG
                     PSPASSDDRVAAE"
     gene            1658..>2365
                     /locus_tag="VG274_06310"
     CDS             1658..>2365
                     /locus_tag="VG274_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWE06302.1"
                     /translation="MNVSQSASNAPAAAPDRTGAPARARGHAHIEPSRRRTALLTLGA
                     LGIVFGDIGTSPFYAMRESVRATGGGAPSHFAILAAASMIFWSLVLVVTVKYVFLIMR
                     ADNDGEGGVLALAALAHRSSGLGRRLKMLIGFGAILGLALFFGDGMLTPAITVLSAVE
                     YVGADVPGLVPLVVPLTLIILVLLFAFQNRGTAKIGRLFGPIMLIWFAVLAVMGFRAI
                     VRTPEILWSLSPYYAIAF"
BASE COUNT          343 a          748 c          804 g          470 t
ORIGIN      
        1 ccgacctcga agttccgggc gagctgccgg agaccgcggc cgcgtggcgg gcgcagcagg
       61 cgcgctggac gaaaggccat gcgcaggtgg cgcgcaagct gctgccgaga atcctgtcga
      121 gccggatgcc tctgtggaag aagctcgtaa tgtcgtttca ggtctgccag ttcgctttct
      181 atacgctcgc ggccgtgagc gccgtgatca gcatcgcgct gatgtcgatg ggggtgacct
      241 atctgcaatc ggtcgccgaa cttggcatcg cggtgacgat catcggggta ggaacgagtg
      301 tcggctatct ctattgcggc cagctcatgc tggggcgggc gggcgaaccg ctgcttccgc
      361 aatctctgct gcttgccatc gtctttccga gcgggctcat cctgtcgaat gcgagagcaa
      421 ccttcgaggc attctggagg agcgatcttg tattcgtgcg cacgccgcgc gccggggagt
      481 tcgcggtcgg cggctggcgg ggccggccgg aaatcgcagc cggacttctt ctccccgtgt
      541 ttgcgctggc cgaacaggca tggagcgcgc ccttcttcgt cttcgccgcg gcggggctgc
      601 tgtcggttgg cgcgatgggc tggaacggca ccggggaagt gcccgcgcgc gaagctcttc
      661 gcctcaagag ccagaattaa tcctgtctta actgtttggc atgcgatgat ttcgcctggc
      721 gcggtcatgg gcagcgaccg gcgcttcgcc ccgacggcag tgtcgcgggc gtgggcattc
      781 gaaggtcgag gcgggaatga agggggcttc tttccgattt gtcgcggtgg ccatgctggc
      841 gcttggcgcg gcaggatgcg cgagcgcgcc ggacgacgat ttcggcagtg gcgaccttcg
      901 cggcctggcg ccttcgaccg aaggcgcgag cgtcgagacg ccctcgacgc ccgttgaatg
      961 cgtgccctat gcgcgggcgc gttcgggcat ttccctctat ggcgatgccg gcacgtggtg
     1021 ggggaaggcc gacggccgct acacgcgaag ccacacgccc ttgctcggct ccgtgattgt
     1081 gctgacggga tatgcggagc ctggccgcgg ccatgtgggc gtggtgagcg ccctcgtctc
     1141 gtcgcgcgag atacggctcg atcacgcaaa ctggctgggc gacggggcca tctatcgcga
     1201 cgatcccgtg gccgatgtca gccccgacaa tgactggacc gaggtccgcg tctggaacac
     1261 ccgcctccac gcctggggcc tcaggaccta ttccgtcgaa ggtttcatcg gtccgtcgcc
     1321 cgcctcatcc gacgaccgcg tcgccgcgga gtgatcggcg gccgcagaag gaggatttaa
     1381 cgcagaggtc gcagaggggg cgcagggcaa ctatagcgcg ccaacggcgc gcactttttt
     1441 caccgcgtcc tcagcgctcc ctctgcgacc cctgcgttaa gccctttctt ttttctggat
     1501 gcgttattct gcccgcccct cttttgtgcg agacgcaggg cgattaggtt ccccgccctt
     1561 caggagggcg gccgggaatg tcggaccgcg cccggcggcg caagcatcca attcgcgtta
     1621 ggctctcagt ccacgaaggg atgacggctc cgccgccatg aacgtttctc aatctgccag
     1681 caatgcccct gcagcggcgc cggacaggac cggggcgccg gcgcgggcgc gcgggcacgc
     1741 ccacatcgaa ccgagccggc gcaggacggc gctgctgaca ctcggcgcgc tcggcatcgt
     1801 attcggcgac atcggcacga gtccgttcta tgcgatgcgc gaaagcgtgc gggcgacagg
     1861 gggcggcgcc cccagccatt ttgcgatcct ggccgcagcc tcgatgattt tctggtcgct
     1921 cgtgctcgtg gtcaccgtca aatatgtgtt cctgatcatg cgcgccgaca atgacggcga
     1981 gggcggcgtg ctcgcgctcg ccgccctggc gcatcgttcg tccggcctcg ggcggcggct
     2041 gaagatgctg atcggatttg gcgcgatcct ggggctcgcg ctgtttttcg gcgacggcat
     2101 gctgacgcct gcgatcaccg tgctgagtgc cgtcgaatat gtcggcgccg acgtccccgg
     2161 acttgtgcct ctcgtcgttc cattgacgct gattatcctc gtcttgctgt tcgcgttcca
     2221 gaaccggggc accgcaaaga tcgggcgctt gttcgggccc atcatgctga tctggttcgc
     2281 ggtgctcgct gtcatgggat ttcgcgcgat cgtccggaca ccggagattc tctggtcgtt
     2341 gagcccttac tacgcaatcg cattt
//
LOCUS       DASZMC010000306         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1512157,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000306 DASZMC010000000
VERSION     DASZMC010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512157"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VG274_06315"
     CDS             complement(<1..1145)
                     /locus_tag="VG274_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06303.1"
                     /translation="MTRPGSLAWFARHELRLSWRDFLSMLTAGRREREPVVALIAFVF
                     VCVMHGVAILALARTGWGAAEADLPTLIAVTATLLLSGSAMLSQAMENVTRAFYSRSD
                     LELILSSPVEAQKLFAVRIGAMALSVATMSLLVVGPFIDVMAWRRGWQWLGAYGVLAA
                     AALTATALAVVLTLALFRAIGPRRTRYAAQILAAVIGGAFVIGLQVAAMFSTGSLSRF
                     AFLRSTTVIMHAPQANSPFWWPARAALGDATCLAITVTAAISLFLAVTVRAAPRFADC
                     AIAASGLTNGATGAHEEGRAFRAGSAAEALRRKEWLLLLRDPWLMSQSLMQLLYLLPP
                     ALLLWRSFAFGGNASAICVPVLIMAAGQLAGGLAWLTISGEDAADLVA"
     gene            complement(1142..1915)
                     /locus_tag="VG274_06320"
     CDS             complement(1142..1915)
                     /locus_tag="VG274_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE06304.1"
                     /translation="MERIGMQEKPSAPALEIRGLRKSFARPAVDGLDLRVRAGEFYML
                     LGPNGAGKTTTLRMVSGLLRPDAGAISVFGIDALADPVAAKRVMGWLSDEPMIYDKLT
                     PLEYLAFVGGLWNVESALAESRARELIGWLGLEAHAHERCEGFSRGMRQKVALAGALV
                     HEPKLVILDEPLTGLDAGAARLVKNVLAQRVRAGNTVIMTTHILEIAERMADRIGVIA
                     HGKLIAEGTLEDLRGQAGRGAATLEDLFLAMVEPGIAAE"
     gene            2219..3064
                     /locus_tag="VG274_06325"
     CDS             2219..3064
                     /locus_tag="VG274_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE06305.1"
                     /translation="MFADIGSARIFFDTVGSGLAIDAERMVEKPTLIVMHGGPGFDHS
                     MMRPYFDRFADTHQVVYIDHRGNGRSTGAPESWMLAQWGDDVKAFCDALGIEKPVVYG
                     NSFGGMVAISYAARHSDHPAKLILSSTAARMRFDDTFRMMEERGGPEARAIAERFWAR
                     MDAPALAEYMRVCMPLYNPGEAEGQAAARRRAILRFDVMHHFALGEIRGMDSRAALAD
                     IRCPVLVMAGTYDPITPVACAEEIHAAVAAGISQLEIFDGAGHGVHRDRPVEAERVLR
                     AFLAS"
     gene            3061..>3733
                     /locus_tag="VG274_06330"
     CDS             3061..>3733
                     /locus_tag="VG274_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE06306.1"
                     /translation="MSAISPRVSPRNVAAAIAGNALEFYDFVTYAFFAVQIGRVFFPA
                     HSEYASLMLSLGTFGAGFAMRPIGGIVIGRYADRVGRKPAMIFSLALMGAAVLAVAVV
                     PSYAAIGVAAPALVVIARMAQGFALGGQVGPTTAFLMEAAPLSQRGFYTAWQGGSQYC
                     ASLAGGIVGILLSTFLDAGQLQAYGWRIAFALGALTLPFGLVLMRNVPETLHRSAPES
                     ASRASG"
BASE COUNT          654 a         1246 c         1184 g          649 t
ORIGIN      
        1 gccacgaggt cggcggcgtc ttcgcccgag atcgtcagcc aggcgagccc gcctgcaagc
       61 tggcccgccg ccatgatgag gaccggcaca caaatggcgg atgcgtttcc gccgaacgcg
      121 aagctccgcc acagcagcaa cgcaggcggc aggagataga ggagctgcat cagcgattgc
      181 gacatcagcc agggatcgcg caacagcagc agccattcct tgcgccggag cgcctccgcc
      241 gcagagccgg cgcgaaaggc gcggccctcc tcgtgtgcgc ctgtcgcgcc atttgtgagg
      301 ccggacgccg caatcgcgca atcggcgaat ctcggcgcgg cccgcaccgt caccgcgaga
      361 aagagcgaga tggctgctgt gacagtgatc gcgagacagg tcgcgtcgcc caaagcagcg
      421 cgcgccggcc accagaacgg actgttcgcc tgcggtgcgt gcatgataac ggtagtcgag
      481 cgcagaaacg cgaatcgcga cagcgacccg gtggaaaaca tcgcggcgac ctgcagcccg
      541 atcacgaatg cgccgccgat cacggccgcc aggatctgcg ccgcgtaccg ggttcgcctg
      601 ggtccaatcg cgcggaacaa ggcgagtgtc agaacaacgg ccagcgccgt tgcggtcagc
      661 gctgcggcgg cgagaacgcc ataggcgcca agccactgcc agccccggcg ccacgccatc
      721 acgtcgatga acggtccgac aacaagcagc gacatcgtcg ccaccgaaag cgccatcgcg
      781 ccgatccgaa cggcaaagag tttctgcgcc tccacgggcg acgacagaat gagttcgaga
      841 tcggagcgcg aatagaaggc gcgtgtcacg ttctccatcg cctgggacag catggcagag
      901 cccgacagca gcaacgtggc ggtgaccgcg atcagggtcg gcaaatcggc ctcggcggcg
      961 ccccatcccg tgcgcgccag cgccagtatc gcaacgccgt gcatcacgca cacgaacacg
     1021 aaggcgatca gcgcaacgac gggctcgcgc tcgcgccggc cagctgtcag catcgagaga
     1081 aaatcgcgcc aggaaagccg caactcgtga cgcgcgaacc aggcgagcga tccgggcctc
     1141 gtcattccgc ggcaatgccc ggttcgacca tggcgaggaa cagatcctcg agggtcgctg
     1201 cgccgcggcc ggcctggccg cgcagatctt cgagcgttcc ttcggcaatc agcttgccgt
     1261 gcgcgatgac gccgatgcgg tctgccatgc gctccgcgat ttccaggata tgcgtagtca
     1321 tgatgaccgt gtttcccgca cgaacccttt gggcgagcac gttcttcacc agccgcgcgg
     1381 cgccggcatc gaggccggtc agcggctcgt cgagaatgac aagtttcggt tcgtggacga
     1441 gagcgcccgc cagcgccact ttctggcgca tgcctcgcga gaaaccttcg cagcgctcat
     1501 gcgcgtgcgc ttcaagaccg agccagccta taagctcgcg cgcgcggctc tcggcgagcg
     1561 cggattcgac gttccacaat cctccgacga aggcgagata ttcgagcggg gtcagcttgt
     1621 cgtagatcat cggctcgtcg gagagccagc ccatgacgcg ctttgccgcg acggggtcgg
     1681 cgagcgcgtc gatcccgaac accgagatcg cgcctgcatc cggacgcagc aatccgctca
     1741 ccatcctgag tgtcgtggtc ttgcctgcgc cgttgggccc caacagcata tagaactcgc
     1801 ccgcccgcac gcgcagatcg agcccgtcga ccgccggccg cgcaaagctc ttgcgcagcc
     1861 ctctgatctc cagcgccggt gccgatggct tttcctgcat tccgatccgt tccattgcgg
     1921 catcttagcg gccagtggtt tcaaagccat aaagcggaaa ggaaggagga aagaggaacc
     1981 gccaagcccc ggattaaatc cggggcaaga cgccaaggca cccgtggatc gcttaagatt
     2041 cacaatgaga cagtgagaag gggaccacgc gccaaaggcg cgcaaatatc ctcactgtct
     2101 ccctgtgaat tctttcttgg cattttggcg ccttggcggt tcacttttcc ttggtacgct
     2161 tgccattggg cggtttgcgt gacgatgatg gggcggcaca ggtgttgaag gctcgttcat
     2221 gtttgcagat atcggcagtg cgcgtatttt cttcgacacg gttggctccg gactggcgat
     2281 cgacgccgag cgcatggttg agaaaccgac actgatcgtc atgcatggcg ggccgggctt
     2341 cgaccattcc atgatgcgac cctatttcga ccgcttcgcc gatacccacc aggtcgtcta
     2401 catcgatcat cgcggcaatg gccgctcgac cggcgcgccg gaaagctgga tgctcgcgca
     2461 atggggcgac gacgtgaagg cgttctgcga tgcgctgggc atcgagaagc ccgtcgtcta
     2521 tggcaattcc ttcggcggca tggtcgcgat ttcctacgcc gcgcgccatt ccgatcaccc
     2581 tgcgaagctc atcctttcct cgacggccgc gcgcatgcgg ttcgacgaca cgtttcggat
     2641 gatggaagag cgcggtggac cagaggcacg tgcaatcgcc gagcgtttct gggcgcgtat
     2701 ggatgcaccc gctcttgcgg aatatatgcg cgtctgcatg cctctctaca atccgggcga
     2761 ggccgagggg caggcggcgg cgcgccgtcg cgcgatcctg cgtttcgacg tcatgcatca
     2821 tttcgcgctg ggcgaaatcc gcggaatgga ttcgcgcgcg gcgcttgctg atattcgttg
     2881 cccggtgctg gtcatggccg gcacgtacga tccgatcacg cccgttgctt gcgcagaaga
     2941 gattcacgcc gcggtcgctg ccggcatctc gcagctcgaa atcttcgacg gcgcgggcca
     3001 tggcgtccat cgcgaccggc cggtcgaggc ggaacgcgtg ctgcgcgcgt tcctcgcctc
     3061 atgagcgcaa tttctccgcg cgtttcgccg cgcaatgtcg ccgccgcgat cgccggcaac
     3121 gcgctcgaat tctacgattt cgtcacctac gcgttcttcg cggtgcagat cggacgcgtc
     3181 ttctttcccg cgcacagcga atatgcgagc ctgatgctgt ccctcggcac cttcggcgcg
     3241 ggcttcgcca tgcgcccgat cggcggtatc gtgatcggac gctatgcgga ccgcgtgggc
     3301 cggaagcccg cgatgatttt cagcctcgcc ctgatgggcg cggcggtcct cgcggtcgcg
     3361 gtcgttccct cctacgccgc gatcggcgtt gccgcccccg cgctggtggt gattgcgcgc
     3421 atggcgcagg gcttcgcgct cggcggccag gtcgggccga caaccgcgtt cctgatggaa
     3481 gcagcgccgc tctcgcagcg cggcttctac accgcgtggc agggcggcag ccagtattgt
     3541 gccagcctcg ccggcggaat cgtcggcatt cttctttcca cctttctcga tgccggccag
     3601 ctccaggcct atggctggcg catcgccttt gcgctcggcg cgctcacgct gcccttcggt
     3661 ctcgtcctca tgcgcaacgt tccggagacg ttgcaccgct ccgctccgga atccgcaagc
     3721 cgcgcgtccg gca
//
LOCUS       DASZMC010000307         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3685395,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000307 DASZMC010000000
VERSION     DASZMC010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3685395"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VG274_06335"
     CDS             <1..858
                     /locus_tag="VG274_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWE06307.1"
                     /translation="LAGGHGLLIGVPGLAKTRLVETLGTVLGLDWKRIQFTPDLMPAD
                     IIGSEILEESETGHRSFRFVRGPVFTQLLMADEINRASPRTQSALLQSMQEKHVTVAG
                     DRHDLPEPFHVLATQNPLEQEGTYPLPEAQLDRFLLQIDVGYPDVDAERRMLIATTFG
                     EEAPIEQACAADALVGAQRIVRHLPVGEKVVEAILRLVRAARPDAGGDAALRGLIAWG
                     PGPRASQAFMLAIRAKALIEGRYAPSVDDVAALAHPVLRHRMALSFSARAEGTAISDV
                     IERLCRSHL"
     gene            855..1790
                     /locus_tag="VG274_06340"
     CDS             855..1790
                     /locus_tag="VG274_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWE06308.1"
                     /translation="MTDAHRRREDSNRAAALRAEAESLGASLPPLLVAAEQLASVVGL
                     GVHGRRKAGSGETFWQFRRYGAGDPANMIDWRQSAKSRHLFVREREWEAAEAVWFWCD
                     PSPGMRFASTPTLDSKIDRAKLLALALASLLIRGGERVALLGKDRAPASGRAALTRMA
                     HGIVEDVSGVEAMPPDLPIGRSAQLVWLGDFLSPLPDIEAAIRRLAGRGLKGHLVHLI
                     DPAEEDFPYTGRTRFESAAAKQSEIFGRAENIRADYCRRFRAQGDAVSAFARRIGWNH
                     LAHRTDQRPETALIALYADLDGSLGFQTRTQSVVR"
     gene            1787..>2255
                     /locus_tag="VG274_06345"
     CDS             1787..>2255
                     /locus_tag="VG274_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HWE06309.1"
                     /translation="MSFLPAISFGAPWILATLAVLPVIYWLLRVTPPAPRRIVFPPTR
                     LLADLAAPEETPARTPPWLLALRLAVAGLIIVALAQPLLGETLALPGKGPLILFIDNG
                     WTAAHDWSSRVAAITDALNSAVHANRAVAIVPAASPAPDAATLLSAGEAERRAA"
BASE COUNT          354 a          761 c          720 g          420 t
ORIGIN      
        1 ctcgcgggag ggcacgggct tctcatcggc gtgccgggcc tcgccaagac gcgactggtc
       61 gaaacgctcg gcaccgtgct tggtctcgac tggaagcgca tccagttcac gccggatctc
      121 atgccggccg acatcatcgg ctcggagatt ctcgaagaaa gcgaaacggg ccatcgttcc
      181 ttccgcttcg tccggggtcc ggtgttcacg cagctgctga tggccgacga aatcaaccgc
      241 gccagtccgc gcacgcagtc cgccctgctg caatcgatgc aggagaaaca cgtgaccgtc
      301 gccggtgacc gtcacgattt gcctgaacct tttcatgtcc tggcgacaca gaaccctttg
      361 gaacaggaag gaacctatcc gcttcccgag gcgcagctgg atcgctttct gctgcagatc
      421 gacgtcggct atcccgatgt ggacgccgag cggcgaatgc tgattgcaac aaccttcggt
      481 gaggaagcgc ccatcgagca ggcctgcgcc gccgacgccc tcgtcggcgc ccagcgaatc
      541 gtccggcatc ttcccgtggg tgaaaaggtc gtcgaggcga tccttcgcct cgtgcgagcg
      601 gcgcgtcccg acgcaggcgg cgatgcggcg ctccgcggcc tcatcgcgtg ggggccggga
      661 ccccgtgcga gccaggcctt catgcttgcg attcgggcaa aggcgcttat cgaaggccgc
      721 tacgcaccct cggtcgacga cgtcgcggcg ctggcgcatc ccgttctgcg tcaccgcatg
      781 gcgctgagtt tctcggcgcg cgccgaaggc accgccatct cggacgtcat cgagcggctg
      841 tgccggagtc atctttgacg gacgcgcatc gtcggcggga agattcgaac agggcggccg
      901 ctctcagggc ggaagccgaa tcgctcggcg catccttgcc gcccttgctc gtcgcagccg
      961 aacagctcgc ctccgtcgtc ggacttggcg ttcatggccg ccgcaaggcg ggaagcggcg
     1021 aaaccttctg gcagttccgg cgatacggcg ccggcgatcc cgcaaacatg atcgattggc
     1081 ggcaatcggc gaaatcacga catctcttcg tgcgcgagcg cgagtgggag gcggcggaag
     1141 cggtctggtt ctggtgcgat ccctcccccg gcatgcgttt cgcttctact ccaactctcg
     1201 attcgaagat cgatcgggcg aagctgctgg cgctggcgct ggcgtcgctt ttgatccgcg
     1261 gcggcgagcg cgtcgcgctg ctgggcaagg atcgtgcgcc tgcgagcggc cgcgccgcgc
     1321 tcacccgcat ggcccacggc atagtcgagg acgtgtcagg cgttgaggcg atgccgcccg
     1381 atctgccgat cggaagaagt gcgcagcttg tttggctggg cgattttctc tcgccgctcc
     1441 ccgacatcga agctgcgatc cgccgtctcg cgggcagagg tctcaaaggg catcttgttc
     1501 atctcatcga tcctgccgaa gaagattttc cctacaccgg acgcacgcgc ttcgaatcgg
     1561 ccgccgcgaa gcaaagcgaa atcttcggcc gcgccgaaaa tattcgcgcc gattactgcc
     1621 gccggtttcg tgcgcagggg gacgcggtga gcgcatttgc gcgccggatc ggctggaacc
     1681 atctcgcgca tcgtaccgat cagcgccccg agactgctct catcgcgctc tatgcggatc
     1741 tcgatggatc actcggcttc cagaccagga cgcaatccgt cgttcgatga gcttcctgcc
     1801 cgccatctcg ttcggtgcgc cctggattct ggcgacgctt gccgtattgc cggtgatcta
     1861 ctggttgttg cgcgtcacgc cccccgcgcc gcgcagaatc gttttcccgc ctacgcgact
     1921 gcttgccgat ctcgccgcgc cggaggaaac gccggcgcgt acgccgccat ggctccttgc
     1981 cctgaggctc gcggtggccg gattgattat cgtcgctctc gcgcaacccc tgctgggtga
     2041 aacgctggct ctgccaggca agggaccgct catcctcttc atcgacaatg gctggacggc
     2101 agcccatgac tggagcagcc gggtggctgc catcaccgat gcactcaatt cggccgtgca
     2161 tgcgaatcgt gccgtggcca tcgttccggc ggcgtcgccc gcaccggacg cggcgactct
     2221 tctttccgcg ggcgaggccg agcggcgcgc cgccg
//
LOCUS       DASZMC010000308         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2047397,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000308 DASZMC010000000
VERSION     DASZMC010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047397"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VG274_06350"
     CDS             <1..259
                     /locus_tag="VG274_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWE06310.1"
                     /translation="LGEAVLGGRVNVPTLTGPVTLTIPAGANSGTSLRLKGKGVPARG
                     GQPAGDLYVRLVVTLPDKPDAALKEFAATWQPDFDPRAKLR"
     gene            403..1260
                     /gene="fabI"
                     /locus_tag="VG274_06355"
     CDS             403..1260
                     /gene="fabI"
                     /locus_tag="VG274_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HWE06311.1"
                     /translation="MTTGKLMHGKRGLIMGVANERSIAWGIAQMLHAHGAELAFTYQG
                     DTFRKRVTPLVEPLQPAALIDCDVTEQGSLDAAFARLQDQWPSLDFLVHAIAFSDKEQ
                     LKGRYIDTTAENFAATMNVSCFSFTAASQRAEKMMPAGGSLLTLTYYGAEKVMPHYNV
                     MGVAKAALEASVRYMAEDLGKRAIRVNAVSAGPIKTLAASGIGDFRYILKWNELNAPL
                     RRTVTTEEVGSAALFLLSDLGRAVTGEILHVDCGYHVVGMKAEDAPDIAVPVRPDSAV
                     PGSARERSA"
     gene            1257..2366
                     /gene="aroC"
                     /locus_tag="VG274_06360"
     CDS             1257..2366
                     /gene="aroC"
                     /locus_tag="VG274_06360"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWE06312.1"
                     /translation="MSYNTFGHLFRVTTFGESHGPALGCVVDGCPPGIPLQESDIQKD
                     LDRRRPGQSKYVSQRKEPDRVRILSGVFQDDRMEAQLTTGTPIALMIENLDARPRDYS
                     DTRDKFRPGHADYTYWAKYGVRDYRGGGRQSARETAARVAAGAIARRVLDFLFPNVFV
                     RGALVQMGPQHIARDNWDWDEVARNPFFCPDAGAVANWAAYLEALRKSGSSCGAVIEV
                     MADGIPAGLGAPIYAKLDTEIAAALMSINAVKGVEIGDGFASALLSGEDNSDEIRKGP
                     DGKPAFLSNRAGGILGGISSGQPIVARFAVKPTSSILKPMRTIDVAGNETEIVTKGRH
                     DPCVGIRAVPVGEAMLACTLADHALLRRAQTGAAP"
BASE COUNT          482 a          805 c          788 g          457 t
ORIGIN      
        1 gctcggagaa gccgtgctgg gcggcagggt caatgtgccc acgctcacgg gtccggtgac
       61 gctcactatt ccggccggag cgaacagcgg cacctcgctt cgactcaaag gcaagggagt
      121 tcccgcgcgt ggcggccaac cggccggcga tctctatgtg cgcctggtcg tgacacttcc
      181 cgacaagcct gacgccgccc tcaaggagtt cgccgcgacc tggcaacccg atttcgatcc
      241 ccgcgcgaaa cttcggtgac ggtctccttg ctttctccac cgatccgccc tagcgggacg
      301 ggacaggtat atcccagcgc acgtctattt tcctccccca acgtggggga ggaaaaccga
      361 atgctcatcg tcggcacgag cctacgcatt gagagggcat aaatgacgac aggcaagctc
      421 atgcatggca agcgcgggct gatcatgggc gtcgcgaatg agcgctcgat tgcatggggt
      481 atcgcgcaga tgctgcatgc tcacggcgcc gaactggcct ttacctacca gggcgatacg
      541 tttcgcaagc gcgtgacgcc gcttgtggag cctttgcagc ccgcggcgct gatcgattgc
      601 gacgtgacgg aacagggctc gctcgacgcg gcctttgccc ggctccagga ccaatggccg
      661 tccctggatt tcctggtcca cgcgatcgcc ttttccgaca aggagcagct taagggccga
      721 tacatcgaca caactgcgga aaatttcgcc gccacgatga atgtctcctg tttctcgttc
      781 acggcagctt cgcaacgcgc cgaaaagatg atgccggccg ggggcagcct gctcacgctc
      841 acctattacg gcgcggaaaa agtgatgccg cactacaacg tcatgggcgt cgccaaggca
      901 gctctcgaag ccagtgtccg ctatatggcc gaggatctcg gaaagcgggc gattcgcgtc
      961 aacgccgttt cggcgggacc gatcaagacc cttgccgcct cgggcatcgg cgatttccgc
     1021 tacatcctga aatggaatga gctgaacgcg ccattgcgcc gcaccgtcac aacagaagag
     1081 gtgggcagcg ccgccctctt ccttctctcc gacctcggcc gcgcggtcac cggagaaatt
     1141 ctgcatgtgg attgcggcta tcacgtcgtg ggcatgaagg cggaagacgc ccccgacatc
     1201 gccgtccccg tcaggcccga cagcgcggta cccggatcgg cacgcgagcg gagcgcgtga
     1261 gctacaacac ctttggacat ctcttccggg tgacgacgtt tggcgagagt cacggaccgg
     1321 ccctcggctg tgtcgtcgac ggatgtccgc cgggaatccc ccttcaggaa tcggacattc
     1381 agaaagatct cgatcgtcgc cgtcccggcc agtcgaaata tgtgagtcag cgcaaggaac
     1441 ccgatcgcgt ccggattctg tccggcgtct tccaggacga ccgcatggaa gcgcaactca
     1501 cgaccggtac gccgatcgcg ctgatgatcg agaatctcga tgcgcggccc agggactatt
     1561 cggatacacg cgacaagttt cggcccggcc acgccgacta cacctattgg gcgaaatacg
     1621 gggtcaggga ttatcgcggt ggcgggaggc aatcggcgcg ggaaacggca gcccgcgtcg
     1681 ccgccggcgc cattgcacgc agagtcctgg acttcctttt cccaaatgtg ttcgtgcgtg
     1741 gggcgctggt gcagatgggt ccgcagcaca ttgcgcgcga caactgggat tgggacgagg
     1801 tcgcacgcaa tcccttcttc tgccccgacg ccggcgccgt tgcgaactgg gctgcctatc
     1861 ttgaagcgct gcgcaagagc ggctcgtcct gcggcgcggt gatcgaggtg atggccgacg
     1921 gaattcccgc agggctgggt gctccaatat acgccaagct cgataccgaa atcgccgccg
     1981 cccttatgag catcaatgcg gtcaaaggcg tggagatcgg cgacgggttc gcctcggccc
     2041 tgctctccgg cgaagacaat tcggacgaga tacggaaggg accggacggc aagcctgctt
     2101 tcctttccaa tcgcgcgggc ggaatcctgg gtggaatatc gagtgggcaa ccgatcgtcg
     2161 cccgatttgc cgtcaagcca acctcgtcaa ttctcaagcc catgcggacc atcgacgttg
     2221 ccgggaatga aactgaaatc gtgacgaagg gccgtcacga tccgtgcgtc ggcatccgtg
     2281 cggtgccggt gggcgaagcc atgctcgcct gcacgctcgc cgaccacgcg ctccttcgcc
     2341 gcgcgcaaac gggcgccgca ccctgagcct gtcgaacgac gccattagag catttttatt
     2401 gattcgcgaa tcgcactggg catcgcgctt cgctcaggag gcccgaactc gctatccgtt
     2461 ttcctccccc atcgtggggg aggtggtttg cgaagcgcag cgaagcaaac cggagggggg
     2521 agtccgcgcg cg
//
LOCUS       DASZMC010000309         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3981953,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000309 DASZMC010000000
VERSION     DASZMC010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3981953"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1220)
                     /gene="dusB"
                     /locus_tag="VG274_06365"
     CDS             complement(108..1220)
                     /gene="dusB"
                     /locus_tag="VG274_06365"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HWE06313.1"
                     /translation="MIWKASSRFDPWAVPAVEDMMCVQSISPLRVGSVSLATNLLLAP
                     IAGYCDVAFRLVARSCGGVGLACTDLLSPEGVLRENKRSMELAATCEEDSPLSMQLYG
                     SDMDRLCEAARWAEDRGADIVDINMGCPVDKVTKRDGGSKLLCDPDRTLRMVQRIVAS
                     LRRVPLTCKLRLGWDDSSLVAPYLAARLEELGVAMITIHGRTTEMRFSGQARLDGIAQ
                     VVAAVKRIPVIGNGDVRTPQDALRMIEQTGCAGVMIGRGALSMPWIFRDTWSYLTRGI
                     IPPPPSIEQKCQHMRDHFYNLIRFRNEHIAVMEFRKRVSWYAKHMTPCRILRDEMREI
                     SNTADFETALQRFLDWRQTPAALRLEHAGAAALCDD"
     gene            complement(1227..1838)
                     /locus_tag="VG274_06370"
     CDS             complement(1227..1838)
                     /locus_tag="VG274_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWE06314.1"
                     /translation="MRLFIRLAMVVLTGAMVSGAPAADWPQFLGPHRNASTTDEGKLA
                     RSWPAGGPKELWHVDLGPGFGAAAIDASGVYVLDRVPKKQEVLRCLDLNTGKELWTCA
                     YEQPAEDVQYSGSRSTPGVDEKCVYTVGCFGDVMCVDKSTHKPLWTHNLVHDFAGKLP
                     HWGVAQSPVLYKDLVIVAPQSGTAGGRTWLFPTASLSCATTRR"
     gene            complement(2016..>2472)
                     /locus_tag="VG274_06375"
     CDS             complement(2016..>2472)
                     /locus_tag="VG274_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06315.1"
                     /translation="KDRLTWDAPLRFGAFAYDASEDYVALHFHNAYRPDSPFDHGEDL
                     RRSLRTIVDDIRARELKIARIGVDSWINSVAPFQSLFPQAFAASLEPTDPDNKGGHGW
                     WGQYISRTGTLNEKRAQRLRETRQFDVVRTHGECAYELFRMHVESASAG"
BASE COUNT          482 a          805 c          729 g          456 t
ORIGIN      
        1 gttccaggcg tcgcggcaag ctcacggtcg gctcctcatc aattcgtcgg gacagttcgc
       61 tggatatgtt ttgggcaggg cgatgtgtat tgacgccgcg gacggaacta atcgtcacac
      121 agcgcagccg cacccgcatg ctccagccgc agcgccgccg gcgtttgccg ccagtccagg
      181 aagcgctgta acgccgtctc gaaatcggcg gtgttgctaa tctcgcgcat ctcatcgcgc
      241 aggattcggc acggggtcat gtgcttggcg taccaactca cccgcttgcg gaattccatg
      301 acggcaatgt gctcgttgcg gaagcgaatc aagttgtaaa agtgatcgcg catgtgctga
      361 cacttctgct cgatgctcgg cggcgggggg atgatgccgc gggtgaggta gctccaggta
      421 tcgcggaaaa tccacggcat ggagagcgcg ccccggccga tcattacccc ggcgcaaccg
      481 gtttgctcga tcatccgcag cgcgtcctgc ggcgtgcgca cgtcgccgtt accgatgacg
      541 ggaatgcgct taaccgcggc gaccacctgg gcaatgccgt cgagccgggc ttggccgctg
      601 aaacgcattt cggtggtgcg gccgtgaatg gtgatcatgg ccacgcccag ttcttccaat
      661 cgcgccgcca gatagggcgc gaccagcgag ctgtcgtccc accccaggcg cagcttgcag
      721 gtgagcggca cccggcgcag cgatgcgacg atgcgctgca ccatgcgcag ggttcgatcg
      781 ggatcgcaca gcagtttgct gccgccgtcg cgcttggtga ccttgtccac cgggcagccc
      841 atgttgatat ccacgatgtc cgccccgcgg tcctcggccc atcgggcggc ctcacagagc
      901 cggtccatgt cgctgccgta aagctgcatc gacaacggcg aatcttcctc gcaggtcgcg
      961 gccagctcca tcgatcgctt gttttcgcgc agcacccctt caggcgagag caggtccgta
     1021 cacgccagcc ccacgccgcc gcacgaacgc gccaccagac gaaacgccac atcgcaatag
     1081 ccagcgatcg gcgccaatag aagattggtg gccagcgaaa cgctgcccac gcgcagcggc
     1141 gaaatggact gcacacacat catgtcttct acggcgggaa ccgcccacgg atcgaaccgg
     1201 ctacttgctt tccagatcaa agcacttcat ctccttgttg tcgcgcacga caagcttgcc
     1261 gttggaaaaa gccatgtccg cccaccggcc gttccgcttt gcggtgcaac gataaccagg
     1321 tccttgtaaa gcaccggcga ttgcgcgacg ccccagtgcg gcagcttgcc ggcgaaatca
     1381 tgcaccaggt tgtgagtcca aagcggctta tgcgtcgatt tatcgacgca catcacatcc
     1441 ccgaagcagc ccacggtata gacgcatttt tcatcgacac ccggcgtgga gcgcgagccg
     1501 gaatactgca catcctccgc aggctgttcg taggcgcacg tccagagttc tttgccggtg
     1561 ttcaaatcca ggcagcgcag cacctcttgc tttttcggca ctcgatccag cacatacact
     1621 ccgcttgcgt cgatcgccgc cgccccaaaa cccggcccca gatccacatg ccaaagctcc
     1681 ttgggcccgc ccgccggcca cgatcgcgcc agcttgcctt catcggtggt cgatgcattc
     1741 cgatgcggcc ccaaaaactg cggccaatcc gcggcaggcg caccggaaac catcgccccc
     1801 gtcagcacca ccatcgccaa ccgaatgaaa agccgcataa atgctccttt aggtaagcaa
     1861 gcatcatcgc ccgcaaaggc cgcgcaggca aggcgacgac tcgctggcgg agtaatccga
     1921 aggttggcct tttcgcgatc tgttgccttt tggcggtatg ggcaaacagt ggataaagta
     1981 agcgccgatc ggcgacgcga tgacttgatc tcacatcaac ctgccgaggc gctttccaca
     2041 tgcatgcgaa acagttcgta ggcgcattcg ccgtgcgtgc gcacgacatc gaactggcgt
     2101 gtctcgcgca ggcgctgggc acgcttttcg tttaatgtgc cggtgcggga aatatattgg
     2161 ccccaccagc cgtggccgcc cttgttgtcg ggatccgtgg gctccagcga tgcggcgaag
     2221 gcctggggaa acagcgactg aaacggggcc accgaattga tccaggaatc gacgccgatg
     2281 cgggcgattt tcagctctcg cgcccgaatg tcatcgacga tcgttcgcag cgatcggcgc
     2341 aggtcctcgc catgatcgaa cggcgagtcg gggcggtagg cgttgtggaa atgcagggcc
     2401 acgtagtctt ccgaagcgtc gtaggcaaac gctccaaatc gaagcggcgc gtcccatgtc
     2461 agccgatcct tc
//
LOCUS       DASZMC010000310         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_121464,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000310 DASZMC010000000
VERSION     DASZMC010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121464"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1802)
                     /locus_tag="VG274_06380"
     CDS             complement(213..1802)
                     /locus_tag="VG274_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HWE06316.1"
                     /translation="MSCAVFKPDSPAREAARRRSFAIISHPDAGKTTLTEHLLLLGGA
                     IRMAGQVKARGEARRARSDWMKIEQERGISVTSAVMTFEYADCVFNLLDTPGHEDFSE
                     DTYRTLTAADSAVMVIDAARGIETQTRKLFEVCRLRDIPIITFINKMDREARDPLELI
                     DEIADQLQLECAPMVWPAGMGLNLKGVWDLDADNFLRFPSGERVGRDTLTGLLSPETL
                     TQLDEEVALARGACPAFDVDIYRTGHLTPVYFGSALKNFGVRELLDALATHAPAPREQ
                     ASRTRTIRPADEEVTGFVFKVQANMDPNHRDRVAFLRLCSGEFRRGMKLKQSGTGRTL
                     GVHNPILFFAQQRETVDDAWPGDVIGIPNHGVLRVGDTLSESGEVVFTGIPNFAPEIL
                     RRVRLGDPMKQKHLTRALESLAEEGVIQVFRPLIGAQWIVGVVGALQLDVLKSRLRAE
                     YGLDADLEASPYDTARWISGPAADMEKFISFHREQMAEDRDKASVFLAKSNWELGYVA
                     QKSPSLAFAKTRERADVHAAG"
     gene            1989..>2553
                     /locus_tag="VG274_06385"
     CDS             1989..>2553
                     /locus_tag="VG274_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06317.1"
                     /translation="MNDDEIDADRGIASGLAMEDASPLEGEPGHAAELRREQVEFLRA
                     QTALHGLHARKLHADLKTVAVANLRERLKLVLDAGLALVGIALIALIGTLVWSAVRSQ
                     SVVVDAFSVPPDFAAQGNTGVVVAGGFLDQLTALQAATRTSAQHHHIEDAWSNDIKVE
                     IPETGVSLGEALTYLRGWLGHDIHIGGS"
BASE COUNT          437 a          825 c          853 g          438 t
ORIGIN      
        1 gagcacgtag agcgttcagg cgaacgaatg cgctgaatgt cgcctccatt ttcctccccc
       61 aacgtggggg aggtgccgag cgaagcgagg cggaggggga tgtgggcgcg cgagccgcgc
      121 gccgactccc ccccggtttg ctccgcttcg ctgcgcaaac cacctccccc acgttggagg
      181 aggaaaacag aacgcaggct agtatggggg agctaccccg cggcgtgcac gtcggcgcgt
      241 tcgcgcgtct tcgcaaaggc gagcgatgga gatttctggg cgacatagcc gagctcccaa
      301 ttgctcttgg cgaggaacac cgatgccttg tcgcggtcct ccgccatctg ctcccgatgg
      361 aacgagatga atttctccat gtcggccgca ggccctgaaa tccaccgcgc cgtatcgtag
      421 ggagaagctt cgagatccgc gtcgagaccg tattccgcgc ggagccggct tttcagcacg
      481 tcgagctgca gtgcgccaac cacgcccacg atccattgcg cgccgatcag cggccggaac
      541 acctggatca cgccctcctc cgcgaggctt tcgagcgcgc gggtcaggtg cttctgcttc
      601 atcggatcgc cgagccgcac gcggcgcaga atttcgggcg cgaaattcgg gatgccggtg
      661 aacaccacct cgcccgattc ggaaagcgtg tcgcccactc tcagcacgcc atgattggga
      721 atgccgatca cgtcgcccgg ccaggcgtca tccacggtct cgcgttgctg ggcgaagaac
      781 aggatcgggt tatgcacgcc gagcgtcctg ccggtgccag actgcttcag cttcatgccg
      841 cgccggaact cgcccgaaca caatcgcagg aaagcgacgc ggtcgcgatg attggggtcc
      901 atattcgcct gcaccttgaa cacgaagccg gtgacttcct cgtccgccgg tctgatcgtg
      961 cgcgtgcgcg aagcctgctc gcgcggcgcg ggcgcgtggg tggcgagcgc gtccagcaat
     1021 tcccgcacgc cgaaattctt cagcgcgctg ccgaaataga ccggcgtgag atggcccgtg
     1081 cggtagatgt ccacatcgaa agcggggcat gcgccgcgcg ccagtgcaac ctcctcatcg
     1141 agttgcgtga gcgtttcggg agacagcaat cccgtcagcg tatcgcggcc gacgcgttcg
     1201 cccgacggaa agcgcaggaa attgtcggcg tccagatccc agacgccttt cagattgagc
     1261 cccatgcccg cgggccacac catcggcgcg cattcgagct gcagctggtc ggcaatctcg
     1321 tcgatcagct cgagcggatc gcgcgcctcg cggtccatct tgttgatgaa ggtgatgatg
     1381 gggatgtcgc gcaggcggca gacttcgaac agcttgcgcg tctgggtctc gatgccgcgc
     1441 gccgcgtcga tcaccatcac cgcggaatcc gccgccgtca gcgtgcggta ggtatcctcg
     1501 ctgaaatcct cgtggcccgg cgtgtccaga agattgaaga cgcaatcggc gtattcgaac
     1561 gtcatcaccg cgctcgtcac cgagatgccg cgctcctgct cgatcttcat ccagtccgag
     1621 cgcgcgcgac gcgcctcgcc gcgcgccttc acctgcccgg ccatgcggat cgcaccacca
     1681 agcagcagca ggtgctcggt cagcgtcgtc ttgccggcat cgggatggga aatgatggca
     1741 aagctccgcc gccgcgcggc ttcccgcgcg ggggaatcgg gtttgaagac ggcgcaactc
     1801 atgggcgcgg ggaggtagca gcgcgggtcg cccggtgcaa ggcggaggcg ccgtcgctcc
     1861 tgatacgatt ggccggtggc gccagcggcg atcgtctttt ggcggctggt cggtttgtct
     1921 gctaacaagg gaagtgtcgg accgctttcc gacaaggggc ggctttcggg ccagggcagg
     1981 ttagcgcaat gaacgacgac gagatcgatg ccgacagggg cattgcaagc ggtctcgcga
     2041 tggaggacgc atctccactc gagggcgagc cgggccatgc cgcggagctg cgccgcgagc
     2101 aggtcgagtt cctgagagcc cagaccgcgc tgcacggcct ccatgccagg aaactgcacg
     2161 ccgacctgaa gaccgtggcg gtggccaatc tccgcgagcg gttgaagctt gtgctcgatg
     2221 cggggctggc gctggtcggc atcgcgctga tcgcgctgat cggaacgctc gtctggtccg
     2281 cggtgcgctc gcaatcggtg gtggtggacg cgttcagcgt gccgccggat ttcgccgcgc
     2341 aggggaacac cggcgtggtg gtcgccggcg gctttctcga tcagctgacg gcgttgcagg
     2401 ccgccacgcg cacctcggcc cagcaccatc acatcgagga tgcctggagc aacgacatca
     2461 aggtcgagat tcccgagacc ggcgtttcgc tgggcgaagc gctgacctat ctgcgcggct
     2521 ggctgggcca cgacatccat atcggcggta gcg
//
LOCUS       DASZMC010000311         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3148762,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000311 DASZMC010000000
VERSION     DASZMC010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148762"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1049)
                     /locus_tag="VG274_06390"
     CDS             complement(198..1049)
                     /locus_tag="VG274_06390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWE06318.1"
                     /translation="MKPLQPSHTAQGAAMHRAAHQLFDRPLVFEDALALAVIGLETEA
                     ELRRGRDWRGHAAPGLRAFIATRSRLAEDLLADAAGGGAGQYVVLGAGLDTYGYRHSQ
                     GRPPLSIFEVDHPATQAWKRARLKEAGLAIPSNVFHAPVDFEREPLDAGLRSAEFDFD
                     KPTFVAWLGVTPYLSRDAVMTTLGFVASHLGKGSEIVFDYAEPADDSDPSHRARFDAL
                     SARVARAGEPFRTFFDPATLAREVKVLGFSDMQDWDAPALNARYFAGRSDGLRIAGRG
                     HCLHARV"
     gene            1147..>1553
                     /locus_tag="VG274_06395"
     CDS             1147..>1553
                     /locus_tag="VG274_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06319.1"
                     /translation="MRSELSQTIGFAEQPADAVAPEREPRSAPGTWAPLVFAIVLGAF
                     WVGAVAAYGWGYFGPGGLFRLNVQESVIAAIAVFVPPMMMLVAAWTFSRGQALAAAAE
                     VMTKATERLLSVDETASRTATRLGRSVRRELDAV"
BASE COUNT          278 a          516 c          493 g          266 t
ORIGIN      
        1 ttgcgcggta ttgtaggcca tgaccagcca gacgatcacg cccagcgagg caagcgagaa
       61 aaggcccaga taggcacgct cgccgatcgc tcctgtgatc ccgtcgcgga tcgtggtgcc
      121 ggcgatgaga agatggatgg ccagaaagac caccgccgcc gcaatcaacg tctgcattgt
      181 gtcccctctt tcccggatca gacgcgggcg tggaggcaat gcccgcgccc ggcgatccgc
      241 agtccgtcgc tgcgcccggc aaaatagcgc gcattgagcg ccggcgcatc ccaatcctgc
      301 atgtccgaaa agccgagcac cttcacctcg cgcgcaagag tcgcaggatc gaagaaagtg
      361 cggaaaggct cgccagcccg cgcgacacgc gccgacaagg catcgaagcg cgcgcggtga
      421 gacgggtcgg aatcatccgc cggctccgca tagtcgaaaa cgatctcgct gccttttccg
      481 aggtgggagg cgacaaagcc gagcgtcgtc atcaccgcgt cgcgcgaaag gtagggcgtc
      541 acgcccagcc aggcgacgaa ggtcggcttg tcaaaatcaa attccgcact tctcagtccg
      601 gcatcgagcg gttcgcgctc gaaatcgacc ggcgcgtgga aaacattcga aggaattgct
      661 aggcccgcct ctttcagccg cgcccgcttc cacgcctgtg tggccggatg atcgacttcg
      721 aagatcgaga gcggcggacg cccctggctg tgccggtagc catacgtgtc gagaccagct
      781 cccagaacca catactgccc cgcgccgcct cctgcggcat ccgcgaggag atcttcggcc
      841 agccggctcc gggtcgcgat gaaggcccgc aatcccggag cagcgtgccc gcgccagtcc
      901 cgcccgcggc gcaattcggc ctcggtttct aacccgataa ccgcaagcgc cagggcgtcc
      961 tcgaacacga gcgggcggtc gaagagctgg tgcgcggcgc ggtgcatcgc cgctccctgg
     1021 gcggtatggc tcggctgaag cggcttcacg ttctattaac cctctgattc cgtttgaata
     1081 gttctcgccg tcggcgggcg ttagggttaa tatgcccgag tcgccgctgt cgaggggacc
     1141 gtgatgatgc gatccgagct cagtcaaact atagggttcg cggaacaacc ggccgatgcc
     1201 gttgcgccgg aacgcgaacc gcgctctgcg cctggaacct gggcgcccct cgttttcgcc
     1261 atcgtcctcg gtgccttctg ggtcggcgcg gtcgccgcgt atggctgggg ctatttcgga
     1321 cccggcggcc tcttccgtct caacgtgcag gaatcggtca ttgcggccat cgctgtgttc
     1381 gttccgccga tgatgatgct cgtggccgcc tggacattta gccgcggaca ggcgctcgcc
     1441 gccgcagcgg aagtcatgac aaaagccact gaacggcttc tttccgtgga cgagacggcg
     1501 tcgcgcaccg ccacccgcct cggccgttcg gtgcgacgcg agctcgatgc ggt
//
LOCUS       DASZMC010000312         6708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2880326,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000312 DASZMC010000000
VERSION     DASZMC010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880326"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="VG274_06400"
     CDS             complement(<1..1401)
                     /locus_tag="VG274_06400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWE06320.1"
                     /translation="MHEPSMAATMSADEPEAALGEERARTAALYREIDLRDRQIAKMR
                     YSGSQDAEALRLSAEIRALQASTSWRITAPLRSIKTRFLSWRRGLPRAPAQSATTRVA
                     RGIPEMPDLQAGEGRGRRRILVVDVRIPAPDIQSSGVRMTAIVDLLREFGFDVMFVSN
                     HGPEDYHWVFANVGDQLRERVAKLHARGVSVIFGYDAAIEHLISKGRCYEAALVCFPD
                     LMLQYAAAIRVYAPQATLIYDTVDLHGVRHRRGAELSKEAEDFRQADYYDRMEAVNVL
                     AADRIVAISAHEASLITHRHPDARVFTIPNIHSAQDRVPGFAQRDGLLFIGHYLHAPN
                     QDAAEHLARDILPLVRAALGDVPLYLVGSSPTEQIKRLTGNGVHVAGHVPNAGQYFDR
                     CRVFAAPLRYGAGMKGKIGQSMSLGLPVVTTSIGAEGMDLENGETALLADNPQDFAAA
                     VIRLYRDEALWRKMSRK"
     gene            complement(1444..2682)
                     /locus_tag="VG274_06405"
     CDS             complement(1444..2682)
                     /locus_tag="VG274_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWE06321.1"
                     /translation="MRRRDFLGCSAAAGGLSLFPFGSGAWALQNPAAGQERLVVVLLR
                     GAIDGLNVVIPYTDAEYYSLRPTIAVAHPGKSGGAIDLDRQFALHPALDSLIPMWKDR
                     SLAFVHACGSPDESRSHFQAQAFMESGTPGVLTTQDGWMNRALAALPGKHAPTEAVNF
                     GKTLPKILGGAMPVASIPTHDYGGRRQGDTSTLDPVTQAVFDRMYAGNDPIGVAYREG
                     RAAHSQLTADLARDMTEAAAGAPSPRGFPQDSAKLCQLMKQDPTIQLAFFALSGWDTH
                     VRQGDAQGQLANHLRPLGEGLAQLRTGLGNVYGRTVVLVMSEFGRTVRENGNGGTDHG
                     HGNAMWIMGGPVNGGKVHGQWPGLDEESLHEQRDLAVTTDFRAVAGAVLKDHLKLTAQ
                     QIDTVFPGSAKSATLNIVHA"
     gene            complement(2708..4195)
                     /locus_tag="VG274_06410"
     CDS             complement(2708..4195)
                     /locus_tag="VG274_06410"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HWE06322.1"
                     /translation="MLETSIALHLSNRLGFGPGQGDLARIAGMGVEAFLDEQLHPQPA
                     RLPPSVTSTLRALPSFGKNSAEAYAEYWWKDQVDDPKSIPKDEKRKLKAREKRVMREA
                     RIARLARAIGSPWQLHEALVEFWFNHFNVYEKKGPIKVWIGAYEEEAIRPHTLGRFSD
                     LLLATAKHPAMLVYLDNAKNVAPGIQATSKVAMADDSMQVPRKNRKERGINENFAREV
                     MELHTLGVDGGYSQADVVALAHILTGWTVGPGADSDAADAVNRFGKNKGAFRFAARRH
                     DPSAESLLGRSFSGGDVGEGEAALLMLAAHPATSRHISFQLAQYFVADQPEPALVGAM
                     AKTFRETSGDIRAVLKTMLNRPEFLAPIRFGAKFKTPYRFVVSAARAAGINPANAAPL
                     AFALEDLGQPLYGCISPDGYACTQAAWLDPDGLLRRLSLAQRIGDGEYGDSKDGAGPP
                     APPDPDRLLDTLKPALGKTTLAAIEQAPRGRRAGAILGSPDFMRC"
     gene            4427..5251
                     /locus_tag="VG274_06415"
     CDS             4427..5251
                     /locus_tag="VG274_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06323.1"
                     /translation="MLNISTTAQHENASLAQSVDPLPRGILCAGAKSSASTWLYNVVE
                     EILRRRGEAEASSYTRADESPLSHAHGNVRQFYADSPRGFSRYDPRVDTLVVQTQQPS
                     DALSALAACPRFQVVMTIREPRDAVASLMKRFGFSFAGAFRAVAEGDARIVGLFRRRA
                     PLVLRFEDRFYEREVTLVAIADFLGVDLPAMLVREIFNALTRESVVKKIESMRQGGVL
                     DDLMNPTRHDPQTRWRLGHVGEIAIGQHAEFLSREQQLVVLTATAEYCAEFGYPAD"
     gene            complement(5261..6472)
                     /locus_tag="VG274_06420"
     CDS             complement(5261..6472)
                     /locus_tag="VG274_06420"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWE06324.1"
                     /translation="MPGNLGFSPARLARIDRFLKDRYVETGKLAGTLFAVSRHGETVH
                     RAAAGLADRERNVPMREDTLFRIYSMTKPISSVAFMMLVEEGLVSLDDPVHRFIPSWR
                     NLGVFVAGVPGAFQTKLASSPMRMIDLLRHTSGLTYGFQARTNVDAAYRKGEIGTIEG
                     DVPLERMIELLGRTPLEFSPGEAWNYSVSTDVLGYLVGSISGIPFEQFLRTRILGPLG
                     MTDTDFHVPEGKHSRLAACYGLDPAGKIVLQDDPTKSRYLAPPAFVSGGGGLVGTAAD
                     YLKFCHMLLNGGVLGGERLLGPRTIAFMTLNHLPGGRELADMSRSLFSEATYAGLGFG
                     LGFAVVLDRARTMTPGNVGEYFWGGAASTAFWIDPTDDIAVVFMTQFLPSTTYPIRQQ
                     LRTLVYAAMEN"
BASE COUNT         1236 a         2107 c         2074 g         1291 t
ORIGIN      
        1 cttgcgggac attttccgcc agagggcttc atcgcggtag agccggatca cggctgccgc
       61 gaagtcctgt ggattatcgg cgagcagcgc ggtctcgcca ttttcgagat ccatgccttc
      121 ggcgccaatc gatgtcgtca cgacgggcag gcccagactc atgctctggc ctatcttgcc
      181 cttcatgccg gctccgtagc gcaggggcgc cgcgaaaacg cggcaacgat cgaaatattg
      241 ccccgcgttt ggcacgtggc ccgcgacatg cacgccgttg cccgtcaatc tcttgatctg
      301 ctcggttggc gagctgccca caagatataa cgggacgtcg ccgagggcgg cccgcacgag
      361 cggaagtatg tcgcgcgcaa gatgctcagc agcgtcctgg ttgggagcgt gcagatagtg
      421 cccgatgaag aggaggccgt cccgctgtgc aaaacccgga acacgatcct gcgcgctatg
      481 gatgttcggg atggtgaata ctcgcgcatc cggatggcgg tgggttatca aactcgcctc
      541 atgggcgctg atagccacga tgcggtcggc cgccaggacg ttcacggctt ccatgcggtc
      601 gtaatagtcc gcctgccgga aatcttcggc ctctttgctc aattcggcgc cgcgcctgtg
      661 tcgtactccg tgcaggtcga ccgtgtcgta gatcagcgta gcctgcggcg cataaacgcg
      721 aattgctgcc gcgtattgga gcatcaggtc gggaaagcag accagggccg cctcgtagca
      781 tcgccccttt gaaatcagat gttcgatcgc ggcgtcgtat ccaaagatca ccgaaactcc
      841 gcgtgcgtgg agcttggcga cccgctcgcg cagctggtcc ccgacattgg cgaagaccca
      901 gtggtagtcc tcgggaccgt gattggaaac gaacatgacg tcgaatccga actcccggag
      961 aagatcgacg atggcagtca tgcgcacgcc ggaactttga atgtcggggg ctggaatacg
     1021 cacatcgacc accagaatcc tgcggcggcc ccgtccttcg ccggcctgca gatcgggcat
     1081 ctcgggtatt ccacgagcta cacgcgttgt cgccgactgt gcgggcgctc gcgggagtcc
     1141 gcggcgccag gaaaggaacc tcgtcttgat gctgcgcaga ggggcggtaa tgcgccagga
     1201 agtggatgcc tggagcgcac gaatttcggc gctcagtctc agggcttccg cgtcctggct
     1261 tccgctgtat cgcattttgg caatctggcg gtccctgaga tcgatttccc gatagagggc
     1321 cgcggtccgc gcgcgttctt cacccagcgc cgcttctggt tcgtcagcgg acatcgtcgc
     1381 cgccattgaa ggttcgtgca tgcaacatgc aaaccgccgc catccatgat ccgaattcga
     1441 tgatcaggcg tgcacgatat tcaatgtcgc gcttttcgcc gagccgggaa acaccgtgtc
     1501 aatctgctgc gcggtcagtt tcagatgatc tttcagcaca gctcctgcga cggcgcgaaa
     1561 atccgtcgtc acggcaagat cgcgctgttc atgcaggctc tcctcgtcga gtcccggcca
     1621 ttggccgtgg actttgccgc cattgaccgg cccgcccatg atccacatgg cgttgccgtg
     1681 gccgtgatcg gtgccgccat tgccattctc gcgcactgtg cggccgaatt cggacatcac
     1741 cagaacgacc gtgcggccgt aaacgtttcc caatcccgtc cgaagttgtg cgaggccctc
     1801 tcccaacggg cgcaaatggt tggcgagctg gccctgggcg tcaccctgcc gcacatgcgt
     1861 atcccagccg ctcaaggcga aaaacgcgag ctgtatggtg ggatcctgct tcatcagctg
     1921 gcagagcttc gcggagtcct ggggaaagcc ccgtggcgac ggggcgcctg ccgccgcctc
     1981 cgtcatgtcg cgcgcgagat cggccgtcag ttgcgaatgc gcggcgcgtc cttcgcgata
     2041 ggcgacaccg atcggatcgt tgccggcata catgcgatcg aacaccgcct gggtgaccgg
     2101 atcgagtgtc gacgtgtcgc cctgcctgcg cccgccatag tcgtgggtcg gaatgctggc
     2161 gaccggcatc gcgcctccaa ggattttggg cagcgtcttc ccgaaattca ccgcttcggt
     2221 cggcgcgtgc tttccaggca gggccgcgag cgcgcgattc atccagccgt cctgggtggt
     2281 cagaacgccc ggcgttcccg actccataaa tgcctgggcc tgaaaatgcg aacgcgactc
     2341 gtccggcgat ccgcacgcgt gaacaaaagc gaggctgcgg tctttccaca tcgggatcag
     2401 cgaatcgagt gccggatgga gcgcgaactg gcggtcgaga tctatggcgc ccccgctttt
     2461 tccagggtgc gccacggcga ttgtcggccg caggctgtaa tactccgcgt ccgtataggg
     2521 aatcacgacg ttcaggccat cgattgcgcc gcgcagcaac acaaccacca gacgctcctg
     2581 tccggcagcc gggttttgca gcgcccacgc gcccgatccg aacgggaaca gcgagagccc
     2641 gccggcggcg gccgagcacc ccagaaaatc gcgacgtcgc atgcaaacct cccgagtggt
     2701 tgcggtttca acagcgcatg aaatccggac ttccgagaat tgccccggcg cggcggccgc
     2761 gtggcgcctg ttcgatcgcc gcaagcgtcg tcttgccgag agcgggtttc agcgtgtcca
     2821 gaagcctgtc cggatcgggc ggcgcaggcg ggcccgcccc gtctttcgag tcgccatatt
     2881 ctccgtcgcc gatccgctgt gcgagagaaa gtcgccgcag gagtccgtcc ggatccagcc
     2941 aggcggcctg ggtgcaggcg tacccgtcgg gagagatgca gccatagagc ggctggccga
     3001 gatcttcgag cgcgaacgcg agcggagcgg cattggccgg attgatgccc gccgcccgcg
     3061 ccgccgatac gacgaaccga tagggcgtct tgaatttggc cccgaaacgg attggcgcaa
     3121 gaaattccgg acggttcagc atggtcttca gcaccgcgcg gatgtcgccg ctggtctcgc
     3181 ggaaggtctt tgccattgca ccgaccaggg cgggctccgg ctgatccgca acgaagtatt
     3241 gcgccagctg aaagctgata tgccgcgacg tcgccggatg ggccgccaac atgagaagag
     3301 cggcctcgcc ctcgccgaca tcgccgccgc tgaagctgcg tccgagcagg gattccgccg
     3361 aaggatcatg acgccttgcg gcgaagcgaa acgccccctt gttcttccca aaccggttga
     3421 cggcatcggc cgcgtccgaa tccgcaccgg gccctaccgt ccagccggtc aggatgtgcg
     3481 cgagtgcgac cacgtcggct tgcgaatagc ctccatcgac cccgagggtg tgcagctcca
     3541 tgacttcgcg ggcaaagttc tcgttgatgc cgcgctcttt cctgtttttt ctggggacct
     3601 gcatggaatc gtcggccatg gcgacctttg acgtcgcttg aatgccgggc gccacattct
     3661 tcgcattgtc gagataaacg agcattgccg ggtgtttcgc cgtcgcaagc aggaggtcac
     3721 tgaatcgtcc aagcgtgtgg ggacgaatcg cctcctcttc ataggccccg atccacacct
     3781 ttatcggacc cttcttctcg tagacgttga aatggttgaa ccagaattcg acaagtgctt
     3841 cgtggagctg ccatgggctt cctatcgccc tcgccagtcg ggcgatgcgc gcctcgcgca
     3901 tgacgcgctt ttcacgcgcc ttgagtttcc ttttctcgtc ctttggaatc gatttggggt
     3961 catccacctg gtccttccac caatattccg cataggcttc ggcgctgttc ttgccgaagg
     4021 acggcagtgc ccggagagtc gacgtcaccg atggcggcaa gcgcgccggc tgaggatgaa
     4081 gctgctcgtc gaggaacgcc tcgacgccca tacctgcgat tcgcgcgaga tctccttgcc
     4141 cgggaccgaa gcccaggcga ttcgaaagat ggagtgcgat agaagtttcg agcatgtcgg
     4201 ggctcgctga agttgatctc accagtatcg gcgaagtgac ccgactctgc cagaggcaca
     4261 tggcggcagt gcgtccgcgc aattaatccc tcgtaacccg cgaggtccag tttgcgggtt
     4321 gactgtctcg ggataaacac ctaaattacc atctacaaga tagacaattg gcgaaaccat
     4381 cctttgccgg gaatcgatcc ctcgaccggg ccagaaaggc gccaacatgc tgaatatctc
     4441 gacgacggct cagcacgaga atgcgtcgct tgcgcagagt gtcgacccgc tcccccgcgg
     4501 aattctgtgc gcaggcgcca agagcagcgc ttcgacctgg ctttacaatg tagtcgagga
     4561 gattcttcgg cgccgcggcg aggccgaagc cagcagctac acgcgcgccg acgaatctcc
     4621 tttgagccat gcccacggaa acgtgagaca gttttatgcg gactctccgc gcggcttttc
     4681 cagatatgat ccccgcgtcg acaccctcgt ggtgcagacc cagcaaccct ccgacgccct
     4741 gagcgctctc gcggcctgcc cccggtttca ggtggtgatg acgattcgcg agccgcggga
     4801 tgccgttgcc tcattgatga aacgattcgg cttttcattt gcgggcgctt tcagggccgt
     4861 cgcggagggc gacgcgcgaa tagtcgggct gtttcgccgc cgcgcgccgc ttgtgctgcg
     4921 cttcgaggac cggttctacg aacgcgaagt gactctcgta gccattgccg attttctggg
     4981 cgtcgatctt cctgccatgc ttgtccggga gatcttcaat gcgctgacac gcgagtcggt
     5041 cgtgaagaaa atcgaatcga tgcgacaggg cggcgtcctc gacgatctca tgaaccccac
     5101 ccggcacgac ccgcagacac gttggcgatt gggccatgtc ggcgagattg caatcggaca
     5161 acatgcggaa ttcctctcgc gtgagcagca actggtcgtg ctgacagcga ctgcggaata
     5221 ctgcgcggaa ttcggttatc cggcggactg acctgctcgg ctaattctcc atcgccgcgt
     5281 agacgagcgt gcgcaattgc tgccggatgg gataggtcgt cgacggcagg aactgggtca
     5341 tgaacacgac ggcgatatcg tccgtcggat cgatccagaa agccgtgctt gccgctccgc
     5401 cccagaaata ctcgccgaca tttccaggcg tcatggtcct cgcccggtcg agcaccaccg
     5461 caaatcccag cccgaatccc aggccggcat aggtggcttc gctgaacagc gaccgcgaca
     5521 tgtctgcaag ttctctcccc ccgggcaggt gattcaacgt catgaacgcg attgtcctgg
     5581 ggccgagcag gcgttcgccg cccagtaccc cgccgttgag aagcatgtgg cagaatttga
     5641 gatagtcggc ggctgtaccg acgagcccgc ctcctcccga gacgaaggcg ggtggagcga
     5701 ggtagcggct ctttgtgggg tcgtcctgaa gcacgatttt tccggccgga tcgagcccat
     5761 agcaagcggc aagccgcgaa tgcttgccct ctggcacatg gaaatccgtg tcggtcatgc
     5821 caagcgggcc gaggattcgc gtgcgcagga actgttcgaa cggtatcccg gaaatcgacc
     5881 cgaccaggta tcccagcacg tcggtcgaaa ccgaatagtt ccacgcctcc ccgggcgaga
     5941 attcgagcgg tgtgcgcccg agaagttcga tcatccgctc gagcgggacg tccccttcga
     6001 tggtgccgat ctcgcccttg cgatacgccg cgtcgacatt cgttcgcgcc tgaaacccgt
     6061 aggtcagacc ggatgtgtgg cgcaatagat cgatcatgcg catgggactt gaggcgagct
     6121 ttgtctggaa ggcgccgggc acgccggcca cgaacacacc cagattccgc cacgacggaa
     6181 tgaagcggtg caccggatcg tcgagagaga cgagaccctc ttcgaccagc atcatgaagg
     6241 cgacgctgga gatgggcttg gtcatcgaat agatgcggaa gagagtatct tcgcgcatgg
     6301 gcacgttgcg ctcgcgatcc gcaagcccgg cggccgcccg atgcacggtc tcgccgtgcc
     6361 gcgaaaccgc aaacagcgtg ccggcgagtt ttccggtttc gacgtagcga tccttgagaa
     6421 atcggtcgat ccgcgccagc cgggcggggg aaaatccgag attgccgggc atggtctata
     6481 tgttcctcgc ggggtggaac gcgttcttgt gttagccgga accccgcccg atgtcactca
     6541 aggcgtttga gcgatcgagc tgatgggccg gagcctgtcc accgcccgct caacaccgtc
     6601 gacgccgccg ttgcccggca aacgtcatgt aatatgctgg gcagctcttg atgaaggatc
     6661 gcctggcata tgagtgcacc ggcttcgagc ctcccgccct ggcctgcc
//
LOCUS       DASZMC010000313         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_944084,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000313 DASZMC010000000
VERSION     DASZMC010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944084"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VG274_06425"
     CDS             <1..435
                     /locus_tag="VG274_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06325.1"
                     /translation="VVSAISKPMEGGVDAAFYEAHRIALGVPKGGIDFAFGETFPHDA
                     NLDLLHGIDFNKGCYIGQEVVSRVEHRGTARKRIVRVRFDGSPPAKGVEIIAGGIALG
                     TMGSAGDGIGLALIRTDRAEEAISAKTPLVAGDRELTLAPAG"
     gene            484..1116
                     /locus_tag="VG274_06430"
     CDS             484..1116
                     /locus_tag="VG274_06430"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWE06326.1"
                     /translation="MTDIAPERPLRPPIRGLDGKMRCPWPGEDPLYIAYHDQEWGVPE
                     WDSRALFEKLILDGFQAGLSWITILRKREAFRRAFDGFEPARMARYNARSVNRLMQDA
                     GIVRNRAKIESSIISARAWIALEAGEGFASFVWKFRDGRPQQNRFRRMGEIPPETETS
                     RKLSKELKSRGFNFCGPTIVYAFMQATGMVNDHLVDCHRHAACAALAKER"
     gene            1334..1750
                     /locus_tag="VG274_06435"
     CDS             1334..1750
                     /locus_tag="VG274_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06327.1"
                     /translation="MGSLDPGAPARWHLAALLHDAPEYVIGDLISPFKTVLGDGYKAV
                     ENRLLAAIHLRFSLPAPPPAALAKVIKRADLAAAFIEATQLAGFAVEEAERFFGRPAI
                     AMRSFEALITPLSAERAQKMFLARFRELEQNEKQDA"
     gene            1775..2287
                     /locus_tag="VG274_06440"
     CDS             1775..2287
                     /locus_tag="VG274_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06328.1"
                     /translation="MPRIHVCSLQRLDETVRRTGARSIVTLIDHGFAVSRPTEIAASD
                     HLCVGISDIVEPVDGYVLPSETHVEQFLGFVRRWDRRDPMVIHCYAGVSRSTAAAFIA
                     ACALAPEYPEQVFATRIRCASPTATPNARLVALADVTLDRKGRMIAAIDAIGRGVDCY
                     EGVPFALDLT"
     gene            2284..>2476
                     /locus_tag="VG274_06445"
     CDS             2284..>2476
                     /locus_tag="VG274_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06329.1"
                     /translation="MNAPPVPPLPVEIGLTAAIVAVAADEPMILVARAGSAVSEATLP
                     SGPFDPVAHRTFEIGLRAWV"
BASE COUNT          452 a          790 c          739 g          495 t
ORIGIN      
        1 gtcgtgtcgg cgatatcgaa acccatggaa ggcggcgtcg acgccgcgtt ctatgaggct
       61 catcgaatcg cgctcggtgt tccaaaaggc ggcatcgact tcgccttcgg cgaaaccttt
      121 ccgcatgacg ccaatctaga ccttctacac ggcatcgact tcaacaaggg ctgctacatt
      181 ggccaggaag tcgtctcgcg ggtcgagcat cgcggcaccg cgcgcaagcg catcgttcgc
      241 gtgcgttttg atgggagccc gcccgccaag ggcgtagaga taattgctgg cggaattgcc
      301 ctcggcacga tggggtcggc cggcgacggc atcggcctcg ccttgatccg cacggaccgc
      361 gccgaagaag cgatctcagc aaagacaccg ctcgtggcgg gagaccggga acttaccctg
      421 gcgccggccg gatagccgcg ctaagcgctt tcctctcgcc caagctgccg ctagatctat
      481 tgcatgaccg acattgcacc cgagcgcccg ctacgccccc cgatccgcgg tctcgacggc
      541 aagatgcgtt gcccgtggcc gggcgaagat ccgctttaca tcgcctatca cgaccaggaa
      601 tggggcgtgc ccgaatggga ttcgcgcgcg ctcttcgaaa agctcattct cgatgggttt
      661 caggccggcc tctcctggat caccattttg cgcaagcgcg aagcattccg tcgtgccttc
      721 gacggattcg agccggcgcg catggctcgc tacaatgcgc gcagcgtcaa tcggctgatg
      781 caggatgccg gcatcgtgcg taaccgcgcc aagatcgaat cctccatcat atcggcacgc
      841 gcctggatcg cccttgaagc gggtgaaggc tttgcatctt ttgtctggaa attcagggat
      901 ggacgaccgc aacagaaccg atttcgccgc atgggcgaga ttccacccga aacagagacg
      961 tcgcgcaaac tgtcgaaaga gctgaagtcc agaggcttca acttctgcgg gcccaccatc
     1021 gtttacgcgt tcatgcaggc gacgggcatg gtcaatgatc acctcgtcga ctgtcaccgc
     1081 cacgcggctt gcgcggcgct cgcgaaggag cgctgaattg gccagatccg catccgctgc
     1141 caaacaaaat tccgcgcgcg cctggcaacg catgctgtcc gggcgccgac tcgatctcct
     1201 tgatccttcg ccgctcgaca ttgaaatcga ggacatagct cacggtctcg ctcgcgtcgc
     1261 gcgttggaac ggccaaacac gcggaccgca gattttttct gtggctcagc attcacttct
     1321 agtcgaaaaa atcgtgggga gtctcgaccc gggcgcgccc gcccgctggc atctcgcggc
     1381 actgctgcac gacgcgcccg aatatgtcat cggcgatctc atctcgccgt tcaaaaccgt
     1441 gcttggcgac ggttacaaag cggtggagaa ccgtttgctg gcggcgatcc atttgcggtt
     1501 ctccctgccg gcgccgccgc cggccgccct tgccaaggtc atcaagcgag ccgacctggc
     1561 ggccgctttc atcgaggcaa cgcaattggc gggctttgct gtagaagagg ctgagcgatt
     1621 cttcggtcgg ccggcaattg cgatgcgaag cttcgaagcc ttgatcaccc ccctttcggc
     1681 agagcgcgcg cagaaaatgt tccttgcccg cttccgcgag ctcgagcaga acgaaaagca
     1741 ggatgcctga acgccctgtt caacggaggt tttggtgccc cgtattcacg tctgttcctt
     1801 gcagcgtctc gacgaaacgg tgcgccgaac cggcgcgcgc agcatcgtta cgttgattga
     1861 tcatggcttc gcggtgtctc gcccgacaga aattgcagcg agcgatcacc tttgtgtggg
     1921 aatttccgac atcgtcgagc cggtcgatgg atatgtgctg ccgagcgaga cgcatgtcga
     1981 acaatttctc ggcttcgtgc gccgatggga ccgccgcgat ccgatggtga tccattgtta
     2041 tgcaggtgtc agccgatcga ccgccgcagc ctttatcgcc gcctgcgcgc tcgcgcccga
     2101 atatcccgaa caggtttttg cgactcgcat ccggtgtgct tcgccgacgg caacgcccaa
     2161 cgctcggctg gtggcgcttg ccgacgtcac gcttgatcgc aagggacgaa tgatcgcggc
     2221 aatcgatgcg atcggccgcg gcgtcgattg ctatgaaggc gtgcctttcg ctttggacct
     2281 tacatgaacg cgccacccgt tcctccgctg ccggtcgaga tcggcctgac tgcagcaatc
     2341 gtcgccgttg ccgccgacga accgatgatt ctggtcgcgc gcgcaggctc tgccgtctca
     2401 gaggccacgc tgccgtcggg cccttttgat ccggttgcgc atcgcacgtt cgaaatcggc
     2461 ctacgcgcat gggtgg
//
LOCUS       DASZMC010000314         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5073654,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000314 DASZMC010000000
VERSION     DASZMC010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5073654"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VG274_06450"
     CDS             <1..431
                     /locus_tag="VG274_06450"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWE06330.1"
                     /translation="KPVACRTAGGRLPGGAIDRVSTVMKVARANGVALPNAGHLARLY
                     GSRLPKLLAILDANPDLCSPLSPSGDIGAQILLAIRGEMALTLEDVVMRRTGIGQEGD
                     PGPMALESAATLMAAECGWSETRRRAEIDSVATIFRNSRA"
     gene            428..1366
                     /locus_tag="VG274_06455"
     CDS             428..1366
                     /locus_tag="VG274_06455"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWE06331.1"
                     /translation="MTGFVVVNPRSAGGRTAREWPQIERALRAAYPHMRLAMTQARGD
                     ATSLVRHALSEGHHEIVAVGGDGTINEAVNGLFNADGPIVPDAVFGFITSGTGGDFRK
                     TFGLGPGYAAAIARLRQAPVHRIDVGRLSCITLKGESTVRYFANIGSFGLSGAVVNAV
                     NRARIAKLFGGRFAFAFHGAAATLSYRDRAVRLRVDGDFDEITRISTVAIANGQFFGG
                     GMRVAPQAIPDDGLFDVVVIAGGSKSRALADMRQIYAGDHLKNPAVRVLRGRRVMAAP
                     VAETRGRPVLIELDGECAGRLPATFELLPRALNLRC"
     gene            1616..3289
                     /locus_tag="VG274_06460"
     CDS             1616..3289
                     /locus_tag="VG274_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWE06332.1"
                     /translation="MAIERSRIRWNGWGWAAHRDALGSREEVWSWLARQLSMPSLLAT
                     PSQRLEDVVLPPSRLPAHDRAQFVALLGSERARDDRDARACHARGKSYHDLLHLRAGL
                     LSTAPDIVLYPRAADETLSILGIAHQSGIAVVPYGGGTSVVGGVSAAAPAFNATATLD
                     LSGMDRVLDIDPQSGTARAEAGIAGPALEITLAEKGVTLGHTPQSFEFSTLGGWIAHR
                     GAGQQSNRYGKAEDWLVAATLATPRGMLVTDSSPASASGPRLVDLVAGSEGIFGVITD
                     ATFRVRAMPEQVEDRAWLFRDFESGIAAIRRAVQGEIPVSMLRLSDADETAFYRGLSD
                     IGAGRSPRRFLARKYLDLRGLDDRAAALIASFEGGADGVASSRSRFAAIVSQLGAVPL
                     GRAPGRRWREGRYRAPYLRDPMLDRGLGVDTFETAATWAKLAGLHAAVREALGKVVAE
                     TAPYPSARGIVMCHLSHSYRDGASLYFTCIFPRALGDEIAQWHAIKNAATDAIMENGG
                     TLSHHHGVGEDHLPWMAREKGELGLDVLRAIKRALDPSGILNPGKLIPS"
     gene            complement(3276..>4064)
                     /locus_tag="VG274_06465"
     CDS             complement(3276..>4064)
                     /locus_tag="VG274_06465"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE06333.1"
                     /translation="DLVAPGPSGAIPLRSYTPQGAAARALPTLIFYHGGGFVIGDLET
                     HDGLCRLLANDSGARVISVDYRLGPEHKFPAAVEDARAAFNWIASNAPRLGVDATRIA
                     VGGDSAGGALATIVAQMAIADRGPRPVFQLLLFPVTDYGADTRSRRAYADGYFLDRRT
                     IAWFFENYVPRGTDLRDPRLSPLYAASVKDMPPAWLLTAGCDPLHDEGIQYAEKLRAA
                     GVPVTIDDHPGLVHDFIYLYGVLPQAGRALRDAALAVRTALTRE"
BASE COUNT          696 a         1321 c         1316 g          731 t
ORIGIN      
        1 ggaagcctgt cgcgtgcagg actgccggag ggcgcctgcc gggcggcgcc atcgaccgcg
       61 tctcgaccgt catgaaagta gcccgcgcga acggcgtcgc acttccgaat gccggtcacc
      121 tggcgcggct ctacggatcc cggttaccga agcttctcgc catcctcgac gccaatccgg
      181 atttgtgctc gccgctttcg ccaagcggcg atattggcgc acagattctt ttggcgatcc
      241 ggggagaaat ggccctgacg ctggaagacg tagtcatgcg gcgcaccgga atcgggcagg
      301 agggcgatcc tggcccgatg gcgctcgaaa gtgccgcgac actcatggct gccgagtgcg
      361 gctggagtga gacgcgcaga cgtgctgaaa tcgactccgt cgcgacaatt ttccggaatt
      421 cgcgcgcatg acgggtttcg tcgtcgtcaa tccacgttcc gccggcgggc gcacggcgcg
      481 cgaatggcca cagatcgagc gtgcgctccg cgccgcttat ccgcatatgc gactcgcgat
      541 gacgcaggcg cgcggcgacg cgacgtcgct ggtgcgccat gcattgagcg aaggtcatca
      601 cgaaatcgtc gcggtcgggg gcgacggcac gatcaacgaa gccgtcaacg gcctcttcaa
      661 cgccgacggt ccgatcgttc ccgacgcggt gttcggcttc atcaccagcg gcacgggcgg
      721 tgattttcga aagaccttcg gtctcggtcc gggttatgcg gcggccatcg cgcggctgcg
      781 gcaggcgccg gtgcatcgca tcgatgtagg acgcctctcg tgcatcacgc tcaagggtga
      841 gagcacggtg cgctatttcg ccaatatcgg ttcgttcggc ctttcgggcg ccgtcgtcaa
      901 tgccgtcaac cgggcgcgca tcgcgaaact gtttggcggg cggtttgcgt tcgcgtttca
      961 tggtgccgcc gcgacgctct cctatcgcga ccgggctgtg cgcctgcggg tcgatggcga
     1021 cttcgacgaa attacccgga tatcgaccgt cgccatcgcc aatggacagt tcttcggcgg
     1081 cggaatgcgc gttgcgccac aggccattcc cgatgatggg ctttttgacg tcgtggtcat
     1141 cgctggaggc tcgaaaagcc gcgcgctggc agacatgcgc cagatttacg cgggcgatca
     1201 cctgaaaaat cccgcggtgc gcgtactgcg ggggcgccgg gtcatggccg cgcccgtcgc
     1261 cgagacccgc ggccggccgg tcctgatcga actcgacggc gagtgcgcgg gacggctccc
     1321 ggccaccttc gagctcctcc cccgcgccct gaatctgcgc tgctagaggt ccagttctaa
     1381 cattagaatc ccaaaaaatt gtcatggcac cgctggtccc cctgttaaac cgggggaggc
     1441 cacccagcga gcgcgcgtcc gcgcgctgaa aaaacgctcg gagcgatctc atctgggtga
     1501 cccgctctga gacgggccat gacaagtttt ggctacgaag tgttggaatt ggaccactac
     1561 actgtgtgga attggcgtaa gagtccttac actgacggtc tttattctgg cgcggatggc
     1621 catcgaacgc agcaggatcc gctggaacgg ctggggctgg gcggcccacc gtgacgcatt
     1681 gggctcccgc gaggaagtct ggagctggct cgcgcggcaa ttgagcatgc ccagcctgct
     1741 cgcgacgccc tcccaaaggc tggaggacgt ggtgcttccg ccgtcgcgtc tgccggctca
     1801 cgaccgcgcg caattcgtcg ctctgctcgg ctccgagcgc gcgcgcgacg accgcgatgc
     1861 gcgcgcctgt cacgcccgcg gaaaaagcta tcacgatctg ctgcacctgc gggcaggttt
     1921 gctgtcgacc gcacccgata tcgtgctcta tccccgcgcc gccgacgaaa cactctccat
     1981 ccttggcatt gcgcaccaga gtggcatcgc ggtcgtgcct tatggagggg gaacaagcgt
     2041 cgtcggcggc gtcagcgcag cggcgcccgc tttcaatgcg acggcgacgc tcgatctttc
     2101 aggcatggac cgtgtcctgg acatcgatcc gcagtcgggc acggcgcgcg cggaagcggg
     2161 cattgccggt cccgcactcg aaataaccct ggccgagaag ggcgtcacac tcggacatac
     2221 gccccaatct ttcgaattct ccacgctggg cggatggatc gcccatcgcg gtgcaggcca
     2281 gcaatccaac cgctacggca aggcggagga ctggcttgtc gctgcgacgc ttgccacgcc
     2341 gcgcggcatg ctcgtaacgg actcatctcc tgcatcggct tcaggccccc gtcttgtcga
     2401 cctcgttgcc ggttcagagg gcatcttcgg cgtcatcacc gatgcaacct tccgcgttcg
     2461 cgcgatgccc gagcaggtcg aagatcgcgc ctggttgttc cgcgatttcg aaagcggcat
     2521 cgccgccatc cgccgcgccg tgcaaggcga gattccggtc tcgatgctgc gtttgtccga
     2581 cgccgacgaa acggcctttt atcgcggact ttccgacatc ggcgccgggc gctctccgcg
     2641 ccggtttctg gcgagaaaat atctcgacct gcgcggcctg gacgaccgcg cggctgcgct
     2701 catcgcaagt ttcgagggcg gcgcggacgg tgtcgcgtcg tcgcgcagcc gctttgccgc
     2761 gatcgtctcg caactgggcg cagtcccgct cggacgcgca ccgggccgac gctggaggga
     2821 ggggcgctac cgtgcgccct atctgcgcga tccgatgctg gaccgcggac ttggcgtcga
     2881 caccttcgag acggcggcga cctgggcgaa gcttgcgggc cttcacgctg cggtgcgcga
     2941 ggcgctcggg aaggtggtgg cagaaaccgc cccttatccc tctgcgcggg gcatcgtgat
     3001 gtgtcatctg agccattcct atcgggatgg cgccagcctt tatttcacct gcatttttcc
     3061 acgtgcgctg ggcgacgaga tcgcgcagtg gcatgcgatc aagaatgccg cgaccgacgc
     3121 catcatggag aacggaggca cactcagcca ccatcatggc gttggcgaag atcaccttcc
     3181 ctggatggca cgcgaaaaag gagaactcgg cctcgacgtc ttgcgcgcca tcaagcgcgc
     3241 gctcgatccc tcgggaattc tcaatccggg taagcttatt ccctcgtaag ggccgttctc
     3301 accgcgagcg cggcgtcgcg caatgccctg cccgcctgcg gcagcacgcc gtagagataa
     3361 atgaaatcgt gtacgagacc gggatggtcg tcgatggtca cgggcacgcc cgcagcccgc
     3421 aatttctcgg cgtattgaat gccctcgtcg tgcaggggat cgcaaccggc ggtcaacagc
     3481 caggccggcg gcatgtcttt cacgctggcg gcataaagcg gagacaggcg cggatcgcgc
     3541 agatcggttc cccgcggcac gtaattctcg aaaaaccagg cgatggtgcg ccggtcgagg
     3601 aaatagccgt ccgcatacgc gcgccttgat cttgtgtccg cgccataatc ggtgacggga
     3661 aacagcagaa gctgaaagac gggtcttggc ccgcgatccg ctatggccat ctgcgcgacg
     3721 atggttgcga gcgcgccgcc cgccgaatct cctcccacgg cgatgcgcgt cgcgtcgacg
     3781 ccgagcctcg gcgcattcga agcaatccaa ttgaacgcgg cacgggcatc ttcgacggct
     3841 gccggaaatt tgtgttcggg tcccagacga taatccaccg agatcacgcg cgcgccgctg
     3901 tcattggcga gcaggcggca gagcccgtca tgggtttcga gatcgccgat cacgaagccg
     3961 ccgccgtgat agaagatcag cgtaggaagc gctctcgcag cagcgccctg cggcgtatac
     4021 gagcggagcg ggatcgcgcc gctcggcccg ggcgccacaa gatc
//
LOCUS       DASZMC010000315         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4262913,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000315 DASZMC010000000
VERSION     DASZMC010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4262913"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VG274_06470"
     CDS             <1..978
                     /locus_tag="VG274_06470"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWE06334.1"
                     /translation="NAENVDEIVFTRGTTEAINLVANSWGAKFLRAGDVILLTEMEHH
                     SNLVPWQLLAERTGARLRFIPVREDGTLALGSLDTLLTPEVKLFAFTHISNSLGTINP
                     VADLCAAARSIGAVSLVDAAQSAGHLPVDVRAIGCDFLALSGHKMCGPTGIGALYGRA
                     EILDGMPPWQGGGEMIQSVTYEGSTFKKPPARFEAGTPNIADAIGLGAAVDYLEGVGR
                     AAIFEHDAQLATYTRSRLEQLPGVRVLGPRERAGLVSFVMEAAHPHDVTTYADQYGLA
                     LRGGHHCNQPLMRKFGLPGTARASFYFYNTHAEVDRMIEILEGAVRFFS"
     gene            975..1433
                     /locus_tag="VG274_06475"
     CDS             975..1433
                     /locus_tag="VG274_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWE06335.1"
                     /translation="MNAELEELYQQVILDHSRRPRNVGEIPDGAVHVHGDNPSCGDEI
                     HLHVKFGADGGVEDVKFTGQGCAISQASASMMTLKVKGRSRSEAEMMLNAFHDLVTKE
                     VADPPKALGDLRLLQGVRKFPQRVKCAMLAWRALEQALAGEGRESASSPR"
     gene            complement(1599..>2375)
                     /locus_tag="VG274_06480"
     CDS             complement(1599..>2375)
                     /locus_tag="VG274_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06336.1"
                     /translation="LPALLQQAAPEGRQVPPATIVTLATRGEQLWVRQDFSAVPAEAF
                     RDAHGQCLLDPPERRELPPPCRGCPELEAFCTKVEITASPAYAWRRLGLVDPLGVPTR
                     RGILFSFFHHGEGLAVAAGLEDESYPIDELVFDLANLRAGPRFAGDDSPYGGRLGGLC
                     QRVYDRAELPGYLDMGVPLDYGAGASEVVRAIVEHGTSRTRLLTESLRQGDIERALVE
                     WRSLLRHVVWAPDYELARWRELKEAAGRLLERSTSPAAKV"
BASE COUNT          418 a          771 c          773 g          413 t
ORIGIN      
        1 aacgcggaaa acgtcgatga aatcgtcttc acccgcggca cgaccgaagc gatcaatctc
       61 gtggccaact cctggggcgc gaaatttctg cgcgcaggcg acgtgatcct cctcacggaa
      121 atggagcatc acagcaatct cgtgccctgg cagcttctcg ccgaacggac tggcgcgcgc
      181 ctgcgtttca tccccgtgcg cgaggatggc acgctggccc tcggctcgct cgacacgctg
      241 ctcacgcccg aggtgaagct ctttgctttc acgcacattt ccaactcgct cgggacgatc
      301 aatcccgtgg cggacctctg cgccgccgcg cgttcgatcg gcgctgtgtc gctcgtggac
      361 gccgcgcaaa gcgccggaca cctgcccgtg gatgtacgcg ccatcggctg cgatttcctc
      421 gcgctctccg gtcacaagat gtgcggaccg accgggatcg gcgcgctcta cggacgcgcc
      481 gagatcctcg acggcatgcc accgtggcag ggcggcggcg aaatgatcca gagcgtgacg
      541 tacgagggca gcacgttcaa aaagcccccg gcgcgtttcg aggcgggcac tcccaacatc
      601 gccgacgcca tcgggctcgg cgcggccgtc gattacctcg aaggggtcgg tcgcgcggcg
      661 atcttcgagc atgacgcgca actcgccact tatacccgga gccggctcga gcaattgccc
      721 ggcgtgcggg tgcttggtcc gcgcgagcgc gctggtctgg tgagcttcgt gatggaggcg
      781 gcgcatccgc atgacgtgac cacttacgcg gaccaatatg gcctcgccct gcgcggtgga
      841 catcattgca accagccgct gatgcgcaag ttcggcctcc ccggcaccgc gcgcgcgagt
      901 ttctatttct acaacacgca tgccgaagtg gatcgcatga tcgaaatcct ggagggcgca
      961 gtgcgcttct tttcatgaac gcggaactcg aagagcttta ccagcaggtc atcctcgacc
     1021 attcgcgccg tccgcgaaac gtgggcgaga ttcccgacgg cgcggtgcat gttcacggcg
     1081 acaatccttc ctgcggtgac gagattcatc tgcatgtgaa gttcggcgcg gacggcggag
     1141 tggaggacgt gaagttcacc ggccagggct gcgccatcag ccaggcttcc gcctcgatga
     1201 tgacgctgaa ggtcaaaggc cgcagccgca gcgaagccga aatgatgctc aatgcctttc
     1261 acgatctcgt gacgaaggaa gtcgccgacc cgccgaaggc cctcggcgat ctgcgtttgt
     1321 tgcagggtgt gcggaaattc ccgcaacggg tgaaatgcgc gatgctggcg tggcgggcac
     1381 tcgaacaagc gttggctggg gaagggcggg agagcgcgtc gtcgccgcgc taacatctcg
     1441 ggacgtcccg ccacggcggg atgcaacatc caacatccaa tggctatgcc gggcgccatt
     1501 tggaagtgga tgttttacgg ttcgccaggt ctcccgtgcg attgaagaaa gacatgtcgc
     1561 tgcgggttcc ttcattgggt gttggatgtt ggatgttcct aaaccttcgc agcaggcgac
     1621 gtggagcgtt ccaaaagccg ccccgccgcc tccttcaact ctctccatcg agccagctca
     1681 taatccggcg cccagacgac atgccgcagc aaactgcgcc actccacgag cgcgcgttcg
     1741 atgtctccct ggcgcagcga ttccgtcagc aagcgcgtgc gcgacgttcc gtgctcgacg
     1801 atcgcgcgca ccacttcgct cgcgccggcg ccgtaatcga gcggcacgcc catgtcgaga
     1861 tagccgggca gctccgcgcg gtcgtaaacg cgctggcaaa ggccgccgag ccgcccgccg
     1921 tacggcgagt cgtcgccggc gaagcgcggc cccgcgcgga gattcgcgag atcgaagacg
     1981 agttcgtcga tgggatagct ttcgtcctcc agccccgccg ccaccgcgag cccttcgccg
     2041 tggtgaaaga agctgaaaag aatgccgcgc cgcgtcggga ctccgagcgg atctacgagc
     2101 ccgagtcgcc gccaggcgta ggccggcgaa gcggtgattt cgactttggt gcaaaatgct
     2161 tccagctccg ggcagccgcg gcagggcggg ggaagctcgc gtctttcggg cgggtcgagc
     2221 aggcactggc cgtgcgcgtc gcggaaggcc tcggccggca ccgcggagaa atcctgccgc
     2281 acccatagtt gttcgccgcg cgtggcgagc gtgacgatgg tcgcgggtgg aacttgtcgt
     2341 ccctccggcg cggcttgttg cagcagcgcg ggcag
//
LOCUS       DASZMC010000316         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4803365,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000316 DASZMC010000000
VERSION     DASZMC010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4803365"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VG274_06485"
     CDS             <1..517
                     /locus_tag="VG274_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE06337.1"
                     /translation="IAAPHLLPYSAGKFALVGFSEGLRAELEEDGIYVTTVCPGLMRT
                     GSPRNAAFKGQHRKEYAWFAIADSLPLASISARTAAKRILRACRHGEAEVMLNLPTRI
                     ATTLHGVFPGMTSELNALIHGMLPEPGGIGTERVPGRESESEWAPSRLTKPSENAAWE
                     NNELGARTTAP"
     gene            659..1759
                     /locus_tag="VG274_06490"
     CDS             659..1759
                     /locus_tag="VG274_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06338.1"
                     /translation="MERILPVPEPPRADPVESAKSAGLIYVSDASPGITRKRRGKSFV
                     YLDPRGKAIRDTDTLLRIRALVIPPAWEQVWICPSPLGHIQAVGRDARGRKQYRYHDK
                     FRAVRDEAKYGRMLAFVRALPRIRRRARADLKKRGMPREKVLAAIVRLLETTLIRVGN
                     EEYAHENKSYGLTTIRNNHAKVRGSTIHFHFRGKSGVEHAIDLNAPRLAKIIRKCQDL
                     PGEELFAYVDDEGVTHDIKSDDVNDYLHEITGQDFTAKDFRTWAGTVLAAQALQEFEQ
                     VDSQARRKKNVVRAVEAVAERLGNTKAVCRRCYIHPQIIESYMDGSLLEQLQHQAEQM
                     LRPLHHLHPEEAAVLVLLTRRLSAEKKSNRAA"
BASE COUNT          366 a          672 c          604 g          274 t
ORIGIN      
        1 gatcgccgcc ccgcatttgc tcccgtacag cgcggggaag ttcgcgctcg tcggcttttc
       61 cgaagggctg cgggccgaat tggaggagga cggcatctac gtcacgaccg tctgcccggg
      121 cctgatgcgc accggcagcc ctcggaacgc ggcgttcaag ggccagcacc gcaaggagta
      181 cgcctggttc gccatcgccg actcacttcc cctggcgtcg attagcgccc gcaccgccgc
      241 caagcgcatc ctccgcgcct gccggcatgg cgaggcggag gtcatgctca acctgccgac
      301 ccgcatcgcg accaccctcc acggcgtctt ccccggcatg accagcgaac tcaatgcgct
      361 gatccacggc atgctgcccg aaccgggcgg gatcggcacc gagcgcgtgc ccggaaggga
      421 aagcgaatcg gagtgggcgc cgtcgaggtt gaccaaaccc tcggaaaacg ccgcctggga
      481 gaacaacgaa ctgggcgccc ggaccaccgc accgtagtcg cgccgacgcg ctctgtcttc
      541 cttcaccgct tgcggcttcg cacctctcct gtccagaaag cgcttcgggg tgaggaaaag
      601 tgcgaagccg caagcggtag aggagaagag tttcgagcgg tagaggagaa aatgcgcgat
      661 ggagagaatc ctgcccgttc ccgagccccc gcgcgccgac ccggtcgaat ccgccaaatc
      721 cgcgggcctg atctacgtct ccgacgcctc ccccggcatc acccgcaagc gcaggggcaa
      781 gagcttcgtc tacctcgacc cgcgcgggaa ggcgatccgc gacaccgaca cgctcctgcg
      841 gatccgcgcg ctggtgatcc cgcccgcctg ggagcaggtc tggatctgcc catcccccct
      901 cggccacatc caggcggttg ggcgggacgc gcgcgggcga aagcagtacc gctatcacga
      961 taagttccgc gccgttcgcg acgaggccaa gtacggccgc atgctcgcgt tcgtccgcgc
     1021 gctgcccagg atccgccgcc gggcgcgggc cgatctgaaa aagcgcggaa tgccgcgcga
     1081 gaaggtgctg gcggcgatcg tccgcttgct ggagacgacg ctcatccgcg tcggcaacga
     1141 ggagtacgcc catgagaaca agtcgtacgg cctgaccacg atccgcaaca accacgcgaa
     1201 ggtccgcggc tcgaccatcc acttccattt ccgcgggaag agcggcgtcg agcacgcgat
     1261 cgacctcaac gccccgcgcc tcgcgaagat catccgcaaa tgccaagacc tgcccggcga
     1321 ggagctcttc gcctacgtag acgacgaggg cgtcacgcac gacatcaagt ccgacgacgt
     1381 caacgactac ctccacgaga tcaccggcca ggatttcacc gccaaggact tccgcacctg
     1441 ggccggcacc gtgctcgccg cccaggcgct acaggagttc gagcaggtcg attcccaggc
     1501 acgccggaag aagaacgtgg tgcgcgcggt ggaggcggtg gccgagcggc tggggaacac
     1561 caaggcggtc tgccgtaggt gctacatcca tccgcagatc atcgaatcct acatggacgg
     1621 gtcacttctc gagcagctac agcaccaggc cgaacagatg ctcaggccgc tccaccacct
     1681 gcaccccgag gaggccgccg ttctcgtcct cctgacccgc cggctctccg cggagaagaa
     1741 atccaatcgc gcggcataag cggccgggcc gcaacgatct cgtttaaagc cgccccctat
     1801 cacgggacgg ctcagagagt ccggtttggc gggcgggatc gctcgcatgc ggtgcgagtc
     1861 gtgctgtcgg ctaaagcgga ccgtacgaga gatgtcattt ccccaagggg agaggc
//
LOCUS       DASZMC010000317         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1258059,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000317 DASZMC010000000
VERSION     DASZMC010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258059"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VG274_06495"
     CDS             complement(<1..640)
                     /locus_tag="VG274_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWE06339.1"
                     /translation="MTEMRLESDSFGRIEVPADRYWGAQTERSRRNFRIGGERMPLPV
                     VHAFGIVKCAAAQVNMALGELDRMRGDAIVRAAREIAEGKLDEHFPLVVWQTGSGTQS
                     NMNANEVIANRAIEILGGEMGSKTPVHPNDHVNKSQSSNDCFPTAMHIAAVTEIHRVL
                     LPGLAGLRAGLDEKTRAFADIVKIGRTHLQDATPITLGQEFSGYAAQVVLGAS"
     gene            complement(670..1149)
                     /locus_tag="VG274_06500"
     CDS             complement(670..1149)
                     /locus_tag="VG274_06500"
                     /inference="COORDINATES: protein motif:HMM:NF016286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HWE06340.1"
                     /translation="MTKDFIGYKALTDAALRNVVRGALKHVERQGLVGAHHFRLSFRT
                     RYPGVEIPEFLLEQYPEEMTIILQHQFWGLKVKENHFEVTLSFRKIPATLAIPFGALT
                     AFVDPGVQFGLNFQPEPEGEASQASRPAIGACNGQPQSADKPAAPGEVVSLDSFRKK"
     gene            1388..1840
                     /locus_tag="VG274_06505"
                     /pseudo
     CDS             1388..1840
                     /locus_tag="VG274_06505"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1865..2178)
                     /gene="ssrA"
                     /locus_tag="VG274_06510"
     tmRNA           complement(1865..2178)
                     /gene="ssrA"
                     /locus_tag="VG274_06510"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            2303..3097
                     /locus_tag="VG274_06515"
     CDS             2303..3097
                     /locus_tag="VG274_06515"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HWE06341.1"
                     /translation="MRQYLDLIQLVLARGIEKRDRTGTGTLSIFGHQMRFDLAAGFPL
                     VTTKKLHLKSIIYELLWFLRGETNVRWLREHGVTIWDEWADAAGELGPVYGSQWRSWP
                     DPHGPTIDQIASVVDEIRRNPDSRRLIVTAWNPADIPKMALPPCHCLFQFYVVQGRLS
                     CQLYQRSADVFLGVPFNIASYALLTMMVAQVSRLEPGEFVHTLGDAHLYSNHLAQARE
                     QLSRTPYPLPQMHLNPAIGDMFAFGFEDFTLQNYRAHPHIRADIAV"
     gene            complement(3147..3779)
                     /locus_tag="VG274_06520"
     CDS             complement(3147..3779)
                     /locus_tag="VG274_06520"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HWE06342.1"
                     /translation="MKLLLISALALGLATVAPAAWAQGENHHQQGGAPHAAPGGGMPN
                     RPASPRMTAPSPMHAAPTTHTSVRPRSSTRTPTTTHHIVRRTVQHTTTTHRHAVATTR
                     HVSAAHRAAALRRSHANIARFRRTMVATRHFHAGPYRAPPGFRYRRWAIGERLPGAYF
                     ARSFWIADFGIYGLFAPPYGYVWVRYGPDAVLIDEYTGEVISVDYGVFID"
     gene            3894..4376
                     /locus_tag="VG274_06525"
     CDS             3894..4376
                     /locus_tag="VG274_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE06343.1"
                     /translation="MTKISIRAADPGDEEAIHSLLYELAIYERLTANFQLTPAIIARD
                     IIGSASPCCCAVAESDGYAVGIATWYPIYSTFGASHGLYLEDLFVRPARRGRGIGMLL
                     LAFLAARAREEGAAYIDWSVLDWNKSAIAFYEKLGAKHVEGWHIFRLSGRRLEELPVR
                     "
BASE COUNT          785 a         1370 c         1397 g          955 t
ORIGIN      
        1 gcgaggcacc gagcaccact tgcgccgcat agccggaaaa ttcctggccg agcgtgatcg
       61 gcgtcgcgtc ctggagatgc gtgcggccga tcttgacgat gtccgcgaac gcacgggtct
      121 tttcgtcgag ccccgcgcgg agccccgcca gtcccggcag aagcacccga tggatttccg
      181 tcaccgccgc aatgtgcatc gcagtcggga agcaatcgtt cgacgactgg ctcttgttga
      241 cgtgatcgtt tgggtgaacc ggcgtcttgg aacccatttc gcccccgaga atctcgatgg
      301 cgcggttggc gatcacctcg ttcgcgttca tgttggactg cgtaccggac ccggtctgcc
      361 acacgaccag cgggaaatgc tcgtcgagtt ttccctcggc gatctcccga gccgcgcgca
      421 cgatcgcgtc gccgcgcatt ctgtccagtt cgcccagcgc catgttcacc tgtgcggcgg
      481 cgcatttgac gatgccgaag gcgtggacga ccggcagcgg catgcgctcg ccgccgatgc
      541 ggaaatttct gcgcgagcgc tcggtctgtg cgccccaata gcggtccgcc ggcacttcga
      601 tgcggccgaa cgagtcgctt tccaggcgca tttccgtcat ctgttgcccg cgaggttcgg
      661 cggcaaggct catttcttgc ggaaggagtc gaggcttacg acttcgccgg gcgcagccgg
      721 cttgtccgcc gattgcggct gcccattgca ggcgccaatg gcgggccgcg acgcctggga
      781 ggcctctccc tccggttcgg gctggaagtt cagtccgaac tgcacgccgg gatccacaaa
      841 agccgtcaag gcgccaaagg gaattgcaag cgtcgcggga attttcctga acgacagcgt
      901 cacctcgaaa tgattttctt tcaccttcag tccccagaac tggtgctgaa ggatgatggt
      961 catttcttcc ggatattgtt cgaggagaaa ctccgggatt tcgactccgg ggtatcgggt
     1021 gcggaaagag agtcggaagt gatgcgcgcc gacgagcccc tgtctttcga catgtttcag
     1081 cgctccgcgc acgacgttgc gcaatgccgc gtcggtcagg gccttgtaac cgatgaagtc
     1141 cttggtcatg ggagccgctg tcgcatccct ttgagactaa gcgcaccgaa ccgcgagtca
     1201 atcggccgaa cggcgcattt ttctgtgcgc aatttcaata tcgcgtggta gtcacagatc
     1261 atcgcccgac atggcaatgg agcccgattc cgacgatttc gaattggcgc gtgcgcccgg
     1321 cgctgtcgaa ctgttccgcg tgttcgggca tcggaattat cgatagtttt tcgcgggtca
     1381 gctcgtctcg ctcatgggca cgtggatgca gtcggtcgcg caaggctggc ttgtctacac
     1441 gctcgcccat tggcctctgc tcctgggact cgcctcattc tgcaggcagg ttcctgtatt
     1501 tttcgtttcg ccgttcggcg gtacaatctc ggaccgcgtc gaccggcgcc gcatgctgat
     1561 cgtgacgcag agcctgtcga tgctgttcct gctgccggcg gcgttcagcc tgatgctgct
     1621 cggtggttcc accaacacga tcttccacct gctttccgaa gaccaagcgc ggccgcatcg
     1681 tcgcactcta tgcaatggct ttcatgggaa tgatgccgtg gggcgcgttg attctcggcg
     1741 cgctcgtcgt ggtggccgcg ggatacgctt ttcggatgtc cggcttcggc ggacaggctt
     1801 ccgatgcgcg gcccaacgtt tgacgagcaa taaggcttga cgaccagtcg gacccattgt
     1861 ggaaagtggg gagcttctgt tgcccggtgc tccccgaaac cgcgcttgcc taattaggca
     1921 gcgagggcca tttcgttatc gttggcacct gtacgatcgg cccgataacg gcggatacca
     1981 taccgagcga aaggtcagcc tttacacgtc cgtcgatcct gtttcgcccc cgccgcagcg
     2041 ctctcttcga gcgctctggt ggaggcgccg ggtaccgccc ccgggtccgg tccgcttatt
     2101 acgcagccgt ttatcgccat agtccgggca agcccgaacg gcacctatat aggcggtccg
     2161 ggtagaaaat tgaagccggc ccggtcgcgc ctggaaaccc tgagcttttc gacgattctg
     2221 cggcgccaag gctgtggcgc cccggccgcg aagtcgtttc aatgatcgcg acgagtcgcc
     2281 gttgcggaca atcggcaatc gaatgcgcca atatctggac ttgatacaat tggtgctcgc
     2341 gcggggcatc gaaaagcgcg accggacggg caccggaacg ctatccattt tcggtcacca
     2401 gatgcgcttc gatctcgcgg ctggctttcc gctggtcacc accaagaagt tgcacctcaa
     2461 atccatcatt tacgagctcc tgtggtttct gcgcggtgaa accaacgtac gctggctgcg
     2521 ggagcatggc gtcaccatct gggacgagtg ggcggatgcg gcgggcgagc ttggaccggt
     2581 ctatgggtcg caatggcgtt cctggcccga cccgcatggc ccaacgatcg atcagatcgc
     2641 ttcggtcgtc gatgaaatca ggcgcaatcc ggactcgcgc cgattgatcg tgacggcctg
     2701 gaatcccgcc gatatcccga aaatggcgct gccgccctgc cactgcctgt ttcaattcta
     2761 tgtcgtgcag ggccggctct cctgccagct ctaccagcgt tccgcggatg tcttcctcgg
     2821 tgtgccgttc aacatcgcgt cctacgcgct cctgacaatg atggtggcgc aggtttcaag
     2881 gctcgaaccg ggagagttcg tgcatacgtt gggcgacgcg catctttatt cgaatcacct
     2941 cgcgcaggcg cgcgaacagc tttcacgcac gccctatccg ttgccgcaga tgcatctcaa
     3001 tcctgcgatc ggagatatgt tcgccttcgg ctttgaggat ttcaccctcc aaaactatcg
     3061 tgcgcatcca catatcaggg cggacatcgc cgtatgacgc gcgcgcgagt cgagtcccgg
     3121 aaatcctctt cctgcgtgca gcgcgttcag tctatgaaca ccccgtagtc cacggaaatg
     3181 acttcgccgg tatattcatc gataaggacg gcgtctggcc catagcgcac ccagacatag
     3241 ccgtatggcg gcgcgaacag tccgtagatg ccgaaatcgg cgatccagaa cgagcgcgcg
     3301 aagtacgcgc ccggcaggcg ttcgccgatc gcccagcggc gataccgaaa gcctggcggg
     3361 gcgcgatagg gaccggcatg gaaatgccgc gttgcaacca tggtgcgccg gaatctcgcg
     3421 atattcgcat gcgaacgccg aagcgcggcc gcgcggtgcg ccgccgatac gtgacgggtc
     3481 gtcgccacgg cgtgacgatg ggtcgtggtg gtgtgctgga ccgtgcggcg cacgatatga
     3541 tgcgtggtcg tgggcgtccg cgtgctggac cttggccgga cagacgtgtg agtggttggc
     3601 gccgcgtgca tcgggcttgg cgcggtcatt cgcggagaag ccggacggtt cggcatgccg
     3661 ccgcccggcg ccgcatgagg tgcgccgcct tgctggtggt ggttttcgcc ctgggcccat
     3721 gctgccggcg cgaccgtggc caagcccagc gctagcgcgg aaatcaatag gagcttcatt
     3781 gggggagcct tcctctgcta aatgacgcaa ccggaatctt ggtcttttgt tccagaaagt
     3841 tgtcaaaaga tatctgtatt gttgagctat ggctcctggt acccggaatg gaaatgacaa
     3901 aaatctcgat tcgggccgcg gacccggggg acgaggaagc gatccattcg ctgctttacg
     3961 aacttgccat ctatgagcgg ttgacggcga attttcagct tacgcccgcg atcatcgcgc
     4021 gagatatcat cggctcggcg tcgccctgtt gctgcgcggt cgccgaatcc gatggctacg
     4081 cagtcggcat tgcgacgtgg tatccgatct attcgacctt cggcgcctcg cacggtcttt
     4141 atcttgagga tttgtttgtg cgccccgccc gccgtgggcg cggaattggc atgctattgc
     4201 tggccttcct tgccgcgcgc gcgcgggagg aaggcgcagc ctacatcgac tggtcggttc
     4261 tggactggaa caaatccgcc attgcattct acgaaaaatt gggcgccaaa cacgtcgagg
     4321 gctggcatat ctttcgcctc tccggtcgga gactggaaga attgccggtt cgatgagtcg
     4381 tatcgcgctg gtgatcgccc gagccgacaa cggtgttatc ggccggcacg gcaaattgcc
     4441 ctggcggctt gccgaagaca tgcgccactt caagggcgtg acaatgggca agccttgcat
     4501 catgggc
//
LOCUS       DASZMC010000318         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_705141,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000318 DASZMC010000000
VERSION     DASZMC010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705141"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VG274_06530"
     CDS             complement(<1..971)
                     /locus_tag="VG274_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWE06344.1"
                     /translation="MQAEVTRFSGFLTLNRTKFFAAPSPCAFLLVRHICIHFYFQTSQ
                     KTARDLRAPFWHISDCNETTELGGAPMSRLIVVSNRVAPTQEGRPAAGGLAIGVLDAL
                     KETGGVWFGWSGETVSEPSAPVIEKQGNVTYATVGLTKRDYDQYYRGFSNATLWPTFH
                     YRNDLSRYDRQEYAGYQRVNATLAKQLKELLKPDDIIWVHDYHLLPFARCLRELGVKN
                     PIGFFLHIPFPVPEVLRTIPPHEELVKAMCSYDVIGFQTEADRQSFVDYVERGQHGTS
                     SEDGMLHAYNRFLKVGAYPIGIYPDAIAKAAEQFTDRKPVRSLRDGMR"
     gene            complement(984..1238)
                     /locus_tag="VG274_06535"
     CDS             complement(984..1238)
                     /locus_tag="VG274_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06345.1"
                     /translation="MPRTGGGLPLEASIMRHPALRRSARHSKRDARPGSDKSEPAASP
                     DPAAQNRVAPDAPPDGEHKQGGVRPEGLEYQRDLGSEQDA"
     gene            complement(1702..>1888)
                     /locus_tag="VG274_06540"
     CDS             complement(1702..>1888)
                     /locus_tag="VG274_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE06346.1"
                     /translation="EADRQLILATLEQCGGVKTRAAEILGISLKTLYNRLVEYGNDAR
                     EEGDAADESRALGGADV"
BASE COUNT          351 a          570 c          586 g          381 t
ORIGIN      
        1 cgcatgccat cgcgcaggct gcgcaccggc ttgcggtcgg tgaactgctc ggccgctttg
       61 gcaatcgcat ccggatagat gccgatcgga tacgcgccca ccttgagaaa gcggttgtac
      121 gcatgcagca tcccgtcttc gctcgatgtg ccatgctgcc cacgctcgac gtaatcgacg
      181 aacgactgtc gatccgcttc tgtttgaaaa ccgatcacgt cgtagctgca cattgccttg
      241 acgagttctt cgtgcggcgg aatcgttcgc aaaacttcag gcaccggaaa cggaatgtgc
      301 aggaaaaatc cgatgggatt tttcacgccc agttcgcgca aacaccgcgc gaacggcagc
      361 aggtgatagt catgcaccca aatgatgtca tcgggcttca gcaactcctt gagctgtttg
      421 gccaaggtcg cgttgacacg ctggtagccc gcatattcct gccggtcgta gcgcgacagg
      481 tcattgcgat agtggaacgt cggccacagg gttgcgttcg aaaagccgcg gtagtactgg
      541 tcatagtcgc gcttggtcag acccacggtc gcgtaggtca cgttgccctg cttttcgatc
      601 accggggcgg acggctcgct caccgtctcg ccgctccagc cgaaccacac tccgcccgtt
      661 tccttcagcg cgtccagcac gccaatggcc agtccacctg ctgcgggacg gccttcctgc
      721 gttggcgcaa cacgattcga taccacgatc agtctgctca ttggtgctcc acctagttca
      781 gttgtttcgt tgcagtcgga tatgtgccaa aaaggtgcac gcaagtcgcg tgccgttttt
      841 tgagatgtct ggaaataaaa atgtatgcaa atgtgacgga cgagcagaaa cgcgcagggc
      901 gaaggcgccg cgaagaattt tgttctgttt agcgtgagga acccggaaaa cctcgtcact
      961 tctgcctgca agccccggca aactcaggca tcctgctccg aaccgagatc acgctgatac
     1021 tccagcccct cggggcgcac gccgccctgc ttgtgctcgc catcgggcgg cgcatcgggt
     1081 gcgacgcgat tctgcgcggc cggatcgggc gacgcggccg gctcggactt gtcgctgccc
     1141 ggccgggcat cgcgcttcga atggcgcgcg gaacgtcgca atgcggggtg tctcatgatc
     1201 gatgcctcca aaggaaggcc gccgccggta cgcggcatga atacagtcta gggtcgcagc
     1261 gggtaaattg ccggtaaaac atgcgccgac tttgtaacaa atacataaat tcgctgcgcc
     1321 ggactgtggt gcagcgcaca tagcatgtca gagttgcatt gacctcgaag gaggctgaac
     1381 cgatcgcctg aaagcctgcg gtgtctgcgt gggctggggc ggcggatcgc caatgggggg
     1441 cgccaccggc tcgaccggtt cgatcggctg gggagcggga ctgggaatgg tgtcgggttc
     1501 agccgggatc ggcggtgccg agggcggctc gatcggttcg gggcgatgcg cgtgcttcac
     1561 aaagggaagc gcgggagcgg cagcgtagtg gggcatgtgc ttatcctcgt ttttgcgttc
     1621 cattggatcg cagcaaggcc tgtgccggta cgcttaccac tgcccctcaa cgggcggacg
     1681 ccgaaggccc gccccgtggc gctagacgtc cgcgccaccg agtgcgcgcg attcgtcggc
     1741 ggcatcgcct tcttcacgtg cgtcattgcc atactcgacg agacggttgt acagcgtctt
     1801 caggctgatg ccgagaatct cggcggcccg cgtcttcact cccccgcatt gttcgagcgt
     1861 cgccagaatc aactgacgat ccgcctcg
//
LOCUS       DASZMC010000319         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_705627,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000319 DASZMC010000000
VERSION     DASZMC010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705627"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1007
                     /locus_tag="VG274_06545"
     CDS             141..1007
                     /locus_tag="VG274_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06347.1"
                     /translation="MISGAGANIGVQTGEDGVILVDSGAAGASEAVLAEIRKLTSKPI
                     YYILNTSADPDTAGGNGVVSKAGQSIFAGVAGPRSDFVKAMTGGAASILAHENVLARL
                     SATSGKSSDGWPTEAFPGARKYLYMNHEGIEVLHQPAAHTDGDSFVHFRASDVVMAGN
                     ILDTTRFPVIDVEEGGTMQGEIDALNRLVELAIPSIPFIFSEGGTLVIPGHGRIYDQA
                     DVVEYRDMIVILRDVIQDMKTRGMTLEQVRAANPTLPYEKQYGAKTGAWTSDQFVEAV
                     YKTVPLKSEAKK"
     gene            1071..>1747
                     /locus_tag="VG274_06550"
     CDS             1071..>1747
                     /locus_tag="VG274_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06348.1"
                     /translation="MTRVQGAAILVVVAMTSMATTVIAMILFALTMTASVARAQGRGP
                     AGPPPAAKVAAPIDLTGYWVSVVTEDWRYRMVVPPKGDYQSMPMTAESRRVADAWDPA
                     KDEAAGEQCKSYGAAGVMRLPERLHITWQDDDTLKMEIDAGTQTRLFHFGNAKPASGE
                     ATWQGDSSARWEGVVRGAQGSGDLRVQTTHMKAGYLRKNGVPYSANATMTEYFDIVHE
                     HTGDQWLV"
BASE COUNT          368 a          447 c          622 g          310 t
ORIGIN      
        1 cgcgggctgc gtctccggga gttttcctgg ccgtggcgct tgtttcagcg gcgggactca
       61 ttctggcaac cggaggcgtg gttgcccgtg cgcaggagcg gggaggcgtc gcggtttatc
      121 aggttcggcc gaatttttac atgatctccg gcgcgggcgc taacattggc gtgcagactg
      181 gagaagacgg cgtgattctg gtggactcgg gagcggcggg cgcgtccgaa gccgtcctgg
      241 cggagattcg caagctgacc agcaagccga tttactacat cctcaacaca agcgccgatc
      301 cggatacggc gggcggcaac ggcgtggttt caaaggcagg gcaatcgatc ttcgcaggcg
      361 tggctgggcc acgctcagat tttgttaagg caatgacggg cggtgcggcg tccatcctgg
      421 cgcacgaaaa tgtcctggcg cggctgagcg cgaccagcgg taaatcaagc gacgggtggc
      481 ccacggaagc gtttcccggc gctcgcaaat atctttatat gaatcacgaa gggatcgagg
      541 tgctgcatca gccggcggcg cacaccgacg gcgatagctt cgtgcacttt cgcgcgtcgg
      601 acgtggtgat ggccggaaac attttggata cgacacggtt tccggtgatc gacgtggaag
      661 aaggcgggac gatgcaaggc gagatcgatg cgctgaaccg gctggtagaa ctggcgattc
      721 cttcgattcc gttcatattt tccgaaggcg ggacgctggt gattcccggc cacggccgga
      781 tctacgatca ggccgatgtt gtggaatatc gcgacatgat cgtgatcctg cgcgatgtga
      841 ttcaggatat gaagacgcgc ggcatgacgc tggaacaggt gagggccgcg aaccccacgc
      901 tgccgtatga aaaacagtac ggcgcgaaaa cgggcgcgtg gacatcggac cagtttgtgg
      961 aagcggtgta caaaaccgtg ccgctgaaga gcgaagcgaa aaagtgatga agggcgaagc
     1021 gaaaaagtaa tgaagagcga accgaaaaaa taatgcgagg aattcggcga gtgacaagag
     1081 tgcagggcgc ggcgattctc gtcgtcgttg cgatgacaag catggcgacg accgtcattg
     1141 cgatgatcct ctttgcgcta acgatgacgg cgagcgtcgc gcgggcgcaa ggacgtgggc
     1201 cggctggtcc gcctccggca gcgaaggtgg cggcgccgat cgatctgacg ggctattggg
     1261 tttcggtggt gacggaggat tggcgttatc ggatggtggt gcctcccaag ggcgattacc
     1321 aaagcatgcc gatgaccgcg gaatcgcgcc gggtggccga tgcgtgggac ccggcgaagg
     1381 acgaagccgc tggcgagcaa tgcaaatcct acggcgcggc gggggtgatg cgcttgcccg
     1441 agcggctcca catcacgtgg caggatgacg acacgttaaa gatggaaatc gacgcgggca
     1501 cgcagacgcg gttgttccac ttcggcaatg ctaaacctgc gagcggcgag gcaacgtggc
     1561 agggcgattc gtcggcgcga tgggagggcg ttgtgcgcgg cgcgcagggc tcgggcgatt
     1621 tgagggtgca gaccacgcac atgaaggcgg gatacctgcg caagaacggc gtgccttaca
     1681 gcgcgaatgc gacgatgacc gaatactttg acatcgtgca cgaacacacg ggcgatcagt
     1741 ggctggt
//
LOCUS       DASZMC010000320         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1783222,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000320 DASZMC010000000
VERSION     DASZMC010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783222"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VG274_06555"
     CDS             complement(<1..181)
                     /locus_tag="VG274_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06349.1"
                     /translation="MLAALALALSGCATMSRTETSKGPREASHLVDPATTPLGRALLP
                     EEQRHPGQSGFDLVSY"
     gene            complement(307..735)
                     /locus_tag="VG274_06560"
     CDS             complement(307..735)
                     /locus_tag="VG274_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWE06350.1"
                     /translation="MESQRHRYWAHALGRICLGVNIFLHGLVRIPSFPQFADHLQHQF
                     QKSFLPPPAVLASAYFIVAAETLVGFLVLIGLGTRPALIAGTLVMLFLQIGSALIQDW
                     SIAGQQLVYIAFYVGLLATVQYDYLSIDSWRRAHRPIPPM"
     gene            complement(761..>1887)
                     /locus_tag="VG274_06565"
     CDS             complement(761..>1887)
                     /locus_tag="VG274_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWE06351.1"
                     /translation="IARRQVGGDRQLAEDVTQEVFSTLARKGGGLLDRAALGGWLCRT
                     AQFVARDVVRKERRRRAREQEAVAMDDHFDSGETADPERLGAMLDGAMADLGAEDRSA
                     VWLRYYEQKSFAQIGACLRVKENAARMRVERALEKLRGTLARRGVQASAVTVALLLDE
                     AAGAPAPAGLAASVAAKALAAGGVPYAWVPLYAFMTSKTLIAMAGAAVGASAVVFATN
                     YRASDASVRAESMALPRQGPTTAALQEQNRQLRQSAKESAAEVDALLQAVDQLRASNA
                     REPALPAQPATVSLTGHVINPSLIPLGAVGSNSLAAVIAFAGGMDGAADRHGVRVSRI
                     RTGGGVDVSTLDLAGGSNRPDGNSFTLEPGDIVYVPIRTK"
BASE COUNT          353 a          652 c          576 g          306 t
ORIGIN      
        1 cataggacac cagatcgaac ccggactggc ccggatgccg ctgttcctcc ggcagcaggg
       61 cccgccccag cggggtcgtc gcgggatcga ccaggtggga cgcctctctt ggccccttgg
      121 aggtctccgt ccggctcatc gtcgcacagc cggaaagcgc caacgccaac gccgcgagca
      181 aggtcctcca tccgctctcc caggggtttc gcatcaaatc agccaccaag gtgggggcga
      241 aagccgaggt cgtaaatagc ggaatgggac tgttttccgc tatcgggtcg catctgagcg
      301 ctaggattac ataggaggga tcggccggtg ggcccgccgc cacgaatcga tcgagagata
      361 gtcatactgg accgtcgcga ggaggccgac atagaaggcg atgtagacca gctgctggcc
      421 ggcgatgctc cagtcctgga tgagcgccga gccgatctgc aggaagagca tgacgagggt
      481 ccctgcgatc agcgcggggc gcgtgccgag cccaatgagc accaggaatc ccaccagggt
      541 ctccgcggcg acgatgaagt acgcgctcgc aagcaccgcc gggggcggca ggaagctttt
      601 ctggaactgg tgctggagat gatcggcaaa ctgcggaaag gaaggaatgc gcacgagccc
      661 gtggaggaag atattcaccc ccaggcagat cctcccgagc gcatgagccc aataccgatg
      721 gcgctgactc tccatcaccc tcaattagac cggattccgt ctacttggtg cggatgggaa
      781 cgtagacgat gtcgccgggc tcgagcgtga aagaattgcc gtccggtcgg tttgagcctc
      841 ccgcgaggtc caacgtgctg acatccacgc cgccacccgt tcgaatccga gaaactcgca
      901 ccccatgccg gtccgccgca ccgtccatgc cgccagcgaa ggcgatgacc gcggccagtg
      961 aatttgaccc gactgcgccg agggggatca acgaagggtt tatgacgtgt cctgtgaggc
     1021 ttacggtagc cggttgggct ggcaacgccg gttcccgcgc attggacgcg cgtagttgat
     1081 ccaccgcttg aagcaacgca tcgacctcgg ctgctgattc cttggcagac tgacgtagtt
     1141 gacggttctg ttcctggagc gccgccgtgg tgggcccttg cctgggcaaa gccatcgatt
     1201 cggcgcgaac ggatgcgtcg ctcgcgcggt agttcgttgc gaacacgacg gcactggcgc
     1261 ccaccgccgc cccggccatc gcgatcagag tcttcgaggt cataaaggcg tacagcggta
     1321 cccaggcata gggtacccct cctgctgcga gtgccttggc ggcaacggaa gccgccaagc
     1381 cagcgggcgc cggcgcaccg gccgcctcgt ccagcagcaa cgcaactgtc actgcggaag
     1441 cctgaacccc gcgcctggcg agtgtgccgc gcaacttctc cagcgcgcgc tccaccctca
     1501 tccgggccgc gttctccttc acgcgaaggc acgcaccgat ttgagcgaac gacttctgtt
     1561 catagtacct cagccatacc gccgaacggt cctccgcgcc caggtccgcc attgcgccgt
     1621 cgagcatcgc gccaagccgc tccggatccg ccgtttctcc gctatcgaaa tgatcatcca
     1681 tcgccaccgc ctcctgttcc cgcgcccgcc gccgccgttc cttgcgcacg acatcccgag
     1741 cgacgaactg ggccgtgcga cacaaccacc cgccgagcgc ggcccggtcg agcagacccc
     1801 caccctttcg cgccaacgtc gaaaacacct cctgcgtcac gtcctccgcc aactgccgat
     1861 ccccgccgac ctgccgccgc gcgatcg
//
LOCUS       DASZMC010000321         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2338727,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000321 DASZMC010000000
VERSION     DASZMC010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338727"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VG274_06570"
     CDS             complement(<1..306)
                     /locus_tag="VG274_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWE06352.1"
                     /translation="MPFEITMPQLTDTMSEGLLVKWMKKEGDKIKPGEVVAEVETDKA
                     TMEMEAERGGTLAHVVTKEGEKVPVGAVIAVLAGPQESSADVKKQYAAKATGGATAAA
                     "
     gene            complement(413..1396)
                     /locus_tag="VG274_06575"
     CDS             complement(413..1396)
                     /locus_tag="VG274_06575"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWE06353.1"
                     /translation="MPEMQFREALRAAMTEEMERDENVFLMGEEVAQYNGAYKVSEGM
                     LDKFGPRRVFDTPISEAGFSGLGIGAAMVGLRPIIEFMSWSFTLVCLDQLVNNAANGR
                     YMSGGQFRVPVTFRGGNGIAHQLGATHSHRMESVYARIPGLIVVAPSTPAEAKGLLKS
                     SIRCDDPVIFLESEKMLNETGEVPEGEFTLPLGVANVERPGKDVTLVSYGRPLQRVVR
                     ASVEHLAQDHGIDVELIDVRTLRPLDTNTIIESVKKTNRCVIIDEDWGYCGMGGGILQ
                     KIQKPCFDYLDAPIEYVHSDEIPVPFNHFLEEAMMPSVDRIVAAVKEVCYR"
     gene            complement(1434..1643)
                     /locus_tag="VG274_06580"
     CDS             complement(1434..1643)
                     /locus_tag="VG274_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06354.1"
                     /translation="MKKDLAELLALDPFLPFVLVMNSGDRYEVRNPDLAMLAESLLYI
                     MKPRSDRRDILRLTEISSLEILGSE"
BASE COUNT          290 a          587 c          555 g          354 t
ORIGIN      
        1 cgccgcggcg gtggcacccc cggtcgcctt cgccgcatac tgcttcttca cgtccgcaga
       61 gctctcctgg ggcccggcca gcaccgcgat cactgcgccg acgggaacct tctccccctc
      121 cttcgtcacg acgtgggcca gcgtcccgcc gcgctcggcc tccatctcca tcgtcgcctt
      181 gtcagtctcg acctcggcca cgacctcacc cggcttgatt ttgtccccct ccttcttcat
      241 ccacttgacc agaaggcctt ccgacatcgt gtcggtcaac tgcggcatcg tgatctcgaa
      301 gggcatggcg attccaaaca gtggtcagtg gtcagtggtc aggggccagc gggcatgccg
      361 gcactttttc ctcttactgg ccactggcca ctcgccactg gccactgctc tctcaccgat
      421 aacaaacttc cttcaccgcg gcgacgatcc ggtcgacgct gggcatcatc gcctcttcca
      481 ggaagtggtt gaaggggacg gggatctcgt cgctgtggac gtattcgatg ggggcgtcga
      541 gatagtcgaa gcagggcttc tggatcttct ggaggatccc gccgcccatg ccgcagtagc
      601 cccagtcctc atcgatgatg acgcagcggt tggtcttttt caccgattcg ataatggtgt
      661 tcgtgtccag cgggcggagc gtgcggacgt cgatcagctc gacgtcgatc ccgtggtcct
      721 gcgccaggtg ctcgacgctg gcgcggacga cgcgctggag cggccggccg tagctgacga
      781 gggtgacgtc tttgcccggg cgctcgacgt tcgccacgcc gagcggcagg gtgaactccc
      841 cttcggggac ctcgccggtc tcgttcagca tcttctcgct ctcgaggaag atgaccgggt
      901 cgtcgcagcg gatgctgctc ttgagcagcc ccttggcctc ggccggcgtg ctgggggcga
      961 cgacgatcag gccggggatg cgggcgtaga ccgactccat ccggtggctg tgggtggcgc
     1021 cgagctggtg ggcgatgccg ttgccgcccc ggaacgtgac cggcacccgg aactgcccgc
     1081 cggacatgta gcgcccgttg gcggcgttgt tgacgagctg gtccaggcag accagggtga
     1141 aggaccagct catgaactcg atgatcggcc gcaggccgac catggccgcc ccgatcccca
     1201 gcccggagaa gccggcctcg ctgatcggcg tgtcgaacac ccggcgcggc ccgaacttgt
     1261 caagcatccc ctccgacacc ttgtaggcgc cattgtactg cgcgacctcc tcgcccatga
     1321 ggaagacgtt ctcgtcgcgc tccatctcct ccgtcatcgc ggcacggagg gcttcacgga
     1381 attgcatttc gggcatggtt cgtgcttcag tgaaaggtga ctagcggccg gcctcactcc
     1441 gagccaagga tctcaagcga ggagatttcc gtgagccgca aaatgtcccg ccggtcggaa
     1501 cggggtttca tgatatagag cagggattca gccagcattg cgagatcagg gttcctgacc
     1561 tcatatcggt cgccgctgtt catcaccaga acgaacggga ggaacggatc cagtgccagc
     1621 agttccgcaa ggtctttctt catgacctat tgtgccgaca ccagagattg aatggagtcg
     1681 agcttaattt gcttcgcgcc gtcccccaca ggcggcgcga cgaggacaat tgtttcgccg
     1741 atggcggcct ggaacctgcg cacgataggg taccgctgtc cgtccg
//
LOCUS       DASZMC010000322         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2338749,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000322 DASZMC010000000
VERSION     DASZMC010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338749"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..365
                     /locus_tag="VG274_06585"
     CDS             183..365
                     /locus_tag="VG274_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06355.1"
                     /translation="MANLGRVAMTHFASLPTKSLEARYWGIPFAERFGSLFRGLTHGS
                     RRLNAPSHIWPQSLRN"
     gene            complement(793..1248)
                     /locus_tag="VG274_06590"
     CDS             complement(793..1248)
                     /locus_tag="VG274_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06356.1"
                     /translation="MSKAAFSVALLFLFSVQAEARPHHHHRYQVFRSAMALFGGDVQS
                     DERIVSSRPSGCPHAFCGCEASLFRFGRIIPQLNLASNWRHFPRSTPATGMAAVRSGH
                     VMILQQHVTGDIWYVHDGNSGGHVTREHPRSIAGYTIVDPGSGSAFKPL"
BASE COUNT          339 a          483 c          511 g          302 t
ORIGIN      
        1 cctgccgacc aggtgctgcg cgcgctggtc ggtcgcccag aaccctgtgc cgcctttgga
       61 agctgtgccg actgcatcga gcgccaagca agtgccgggc atcacggcgg agccgcaggt
      121 tccggcgccg atcttccatc ccggcgaggt gatcgccgat ctgccgccgg cggcgcgcca
      181 ggatggccaa cctcggccgc gtcgcgatga cgcactttgc gagcctgccg accaagtcgc
      241 tcgaggcgcg ttactggggg attccgttcg ccgagcggtt cggctcgctg tttcgcggcc
      301 ttacgcacgg gtcgaggcgc ttgaacgcgc cgtcgcatat ctggccgcag agcttacgaa
      361 actgaagggt gcgcagcccg gacgcctgcc ggcgctgccc catgtagcgc gcgaaaacat
      421 tccccagatc ctcggacagc cgggcggcat gggacggcct gcggcgatgg gtcctggcgg
      481 caacggcggg acgtgcgccg cttggcgcgg ggcgcagatg gcagtgcgtc ggcttcggcg
      541 ccctcaaccg tggagcaggg atttacgagg aggcaacatc cgctcgacgt gatcggctcg
      601 tcgggcagac gtggctaagg tggagttgca tatggagagc tcggtctttg gatcgcgcgc
      661 tgcctcgccg tgggcagtct gaaatcgaac ccggcgaggt gatgaactga tggctatgat
      721 caataacatt gtcgcccagc tcaggacggc cgtttcgatt agtttcgata acgtcagaac
      781 tcggggctgt cctcataacg gtttaaaagc ggaccccgag cctggatcaa caatggtgta
      841 cccggcaatt gagcgtgggt gttctctagt cacgtggccg ccggaattgc cgtcatgcac
      901 gtaccagatg tcgccggtaa cgtgctgctg caagatcatg acatggcctg atcgaaccgc
      961 cgccatacct gtcgccgggg tggatcttgg aaagtggcgc caattggaag cgagattcaa
     1021 ctgaggaata atccgaccaa aacggaacag tgacgcctcg cacccgcaga acgcatgggg
     1081 acatcctgaa gggcgactgc tcacgattct ttcgtcgctt tgaacgtcac cgccgaagag
     1141 cgccatggcg gagcgaaata cttgatagcg gtggtgatgg tggggacggg cctcggcttg
     1201 aacggaaaaa agaaacaaca acgctacaga aaatgcggct ttcgacatcg aaagggactc
     1261 catgagtttg cgaaatcccc tgccggttaa aattcaaatg aagaaacgtg gcagtatgtt
     1321 ggccaaagca aaccgcacaa tgtccgatgt cagacatacg ggccggatgt caggcgctga
     1381 tttgctgcac gaacgttgat catcctgcgg gcctgaccca acaatcttcg caacctcacc
     1441 aatctctgca acatccgcag tcctgatgtg gtgagcacag gccgacactc ggataatgcc
     1501 cgcattgttc gcccggaaag cgagcgacgc ggatgcccat cccaaatcca tggcgtccgg
     1561 cgatctaacg atcaccctta gccgcgtcgg gtctttccgg gccggacgtc atggaaatcc
     1621 gccatgcgca aactt
//
LOCUS       DASZMC010000323         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3441025,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000323 DASZMC010000000
VERSION     DASZMC010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3441025"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1592)
                     /gene="rpoN"
                     /locus_tag="VG274_06595"
     CDS             complement(72..1592)
                     /gene="rpoN"
                     /locus_tag="VG274_06595"
                     /inference="COORDINATES: protein motif:HMM:NF004596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWE06357.1"
                     /translation="MALTQRLELRQGQTLVMTPQLQQAIKLLQLSNLDLAAYVEQELE
                     RNPLLERDESADGAAEPQETPDAPASDGEALDTALAREDFSKTEELDAEREDVFAEDS
                     APPIDRPLADWTNLRASPLAGGDENGIERVLARSATLQDHLLEQLSIAGLDVARRLIA
                     ISLIDSIDEAGYLRVEAGEIATRLGAAAEEVESVLRVVQGFEPTGIGARNLSECLALQ
                     LRERGRLDPAMNVLVSHLDLVARRDIAGLCRHCAVDAEDVSDMIAELRALTPKPGLAF
                     GSEPVQPVVPDVFVREAPDGGWHVELNADTLPRLLVSVRYYAKIAGRARDKDAKTYLT
                     ECLNSANWLVKSLDQRARTILKVASEIVRQQDAFLAHGVRHLRPLNLRAIADAISMHE
                     STVSRVTSNKYIATPRGVFELKYFFTAAIQATNGAEAHSAESVRDRIRELVLNEAAAQ
                     ILSDDRIVEILTGDGVSIARRTVAKYREALRIPSSVERRRLKKIDVRQPEFQAALR"
     gene            complement(1620..2411)
                     /gene="lptB"
                     /locus_tag="VG274_06600"
     CDS             complement(1620..2411)
                     /gene="lptB"
                     /locus_tag="VG274_06600"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWE06358.1"
                     /translation="MTTASPVLNDERREHERVRLVEGSQGLVVTRIGKSFRRRPVVRS
                     VSLSLRRGEVVGLLGPNGAGKTTCFYMITGLVPADYGTIELDGHDVTKLPMYRRARLG
                     IGYLPQEASIFRGLTAEQNIRATAELVESDPSRLESLVEDLLAEFGIAHVRDTPAIAL
                     SGGERRRVEIARALATHPSYILLDEPLAGIDPIAVTEIRNLVRHLKDRGIGVLITDHN
                     VRDALDIIDRAYIIHEGQVLKEGLPGEIVGDRDVRRVYLGERFSL"
     gene            complement(2452..>2918)
                     /locus_tag="VG274_06605"
     CDS             complement(2452..>2918)
                     /locus_tag="VG274_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HWE06359.1"
                     /translation="AVNLGQHNANAPINVSSDNFVGDFQTKVGTYIGNVIVTQAEYKL
                     HADRVKVNAAQGKPNRFEATGNVVFVSSSGTATGDNGIYDLGPRTVTLTGHVVLTKDK
                     DVMHGTALVVNMVTGVAHLTAHGAPGNRVQGLFIPPPQSSAPGGKPQSGAEQ"
BASE COUNT          531 a          933 c          905 g          549 t
ORIGIN      
        1 gttgagacgg aggcggctcg aaaaaagcgg cggaccatag gtcgcgaccc ctaccctgtc
       61 aattgtagaa atcatcgaag cgccgcctgg aattccggct gccggacgtc gatctttttg
      121 agcctgcgcc gttccaccga cgaaggaatt cgcaacgctt cccgatactt cgcgacggtg
      181 cgcctcgcaa tgctgacgcc gtcgcctgtg aggatttcca cgatgcggtc gtcggacagg
      241 atctgcgcgg ccgcttcgtt caggacgagt tcgcgaatgc ggtcgcgcac cgattcggcg
      301 gaatgcgcct ccgcgccatt cgtcgcctgt atcgccgccg tgaagaaata cttgagttcg
      361 aacacgccgc ggggagtggc gatatatttg ttcgacgtca cgcggctgac cgtcgattcg
      421 tgcatggaga tcgcgtcggc aatggcgcgc agattgagcg ggcgcagatg gcgcacaccg
      481 tgggcgagaa acgcatcctg ctggcgcacg atctcgctcg ccaccttgag aatggtgcgc
      541 gcgcgctgat cgaggctttt cacgagccag ttggcgctgt tgagacattc ggtgagatac
      601 gtcttcgcgt ccttgtcgcg cgcgcgcccg gcaatcttcg cataatagcg cacactcacg
      661 agaaggcgcg gcagcgtgtc ggcgttcagc tcgacgtgcc agccgccgtc tggcgcctcg
      721 cgcacgaaga cgtcgggaac gaccggctgc accggctcgg acccgaaggc caggcccggt
      781 ttcggcgtca gggcgcgaag ctcggcaatc atgtcggaga catcttcggc atcgacggcg
      841 caatgcctgc acaacccggc aatgtcgcgc cgcgcaacga gatccagatg cgacaccaga
      901 acgttcatcg cgggatccag tcgtcccctc tcgcgcaatt gcaacgccag gcattcggaa
      961 aggttgcgcg cgccgatccc cgtcggctcg aagccctgca ccacgcggag aaccgattcg
     1021 acctcctcgg ccgcggcgcc gagccgggta gcgatctcgc cagcttccac tctcaaatac
     1081 cccgcctcgt cgatcgaatc gatcagggag attgcgatca gacgtcgcgc aacatctagg
     1141 ccggcgatgc tcagctgctc cagcaggtga tcctgcaacg tggccgatcg cgcgagcacg
     1201 cgctcgatcc cgttttcgtc gccgccggca agcggagagg cgcgcagatt cgtccagtcg
     1261 gcaagcggtc tgtcgatcgg cggcgccgag tcttcggcga aaacatcctc ccgttctgcg
     1321 tccagctcct cggtcttcga gaaatcttcg cgcgcgagtg ccgtgtccag agcttcaccg
     1381 tcgctggccg gggcgtcggg agtttcctgc ggctccgcgg cgccatccgc actctcgtcg
     1441 cgctcgagaa gcggattgcg ctcgagttcc tgctcgacat aggcagcgag atcgagattc
     1501 gacaattgca gcagcttgat cgcctgctgc aattgcggag tcatgacgag cgtctggccc
     1561 tggcgaagct ccagccgttg cgtcagcgcc atgacatgtc cgcgcccccc ggccgttcgc
     1621 tacagcgaga accgttcgcc gagataaacc cgacgcacgt cgcgatcgcc cacgatctct
     1681 ccgggcagac cctccttcaa cacctgacct tcgtggatga tgtaagcgcg gtcgatgatg
     1741 tccagcgcat cgcgaacatt gtgatcggtg atgagaacac cgatgccgcg atccttgagg
     1801 tgccgcacca ggttgcgtat ttccgtcacc gcgatcggat cgatgccggc aagcggctcg
     1861 tcgagaagaa tgtaggaggg atgggtcgca agcgcgcggg cgatctcgac gcggcggcgc
     1921 tcgcctccgg aaagcgcgat cgccggcgta tcgcgcacat gtgcaattcc gaattccgcc
     1981 agcagatcct cgaccagcga ctccagccgc gacggatcgg attcgacgag ctcggcggtc
     2041 gcgcggatgt tctgttcggc cgtcagtcca cggaagatcg aggcctcctg cggcagatac
     2101 ccgataccga gccgggcgcg gcgatacatc ggaagcttgg tcacgtcgtg accgtccagt
     2161 tcgatcgtgc cgtaatcggc cgggacaagc ccggttatca tgtagaagca ggtcgtcttt
     2221 ccggcgccgt tcggtccgag aagtccgacc acttcgccac gccgcaggga caggctgacc
     2281 gagcgcacga ccggacgccg gcgaaagctc ttgccgatgc gcgtcaccac gaggccctga
     2341 ctgccctcta cgagacgcac tctttcgtgc tcgcggcgct cgtcgttgag aacgggtgaa
     2401 gctgtcgtca tcgcaagaac tgtgcctttt cgactttaag gaagcatgaa cctattgctc
     2461 cgcgcccgat tgcggttttc cgcccggagc cgaagactgc ggcggcggaa tgaagagacc
     2521 ctgtacgcga ttgcccggcg cgccatgcgc tgtcaaatgc gctacgccgg taaccatgtt
     2581 gacgacgaga gccgtgccat gcatcacgtc cttgtctttg gtcaggacga catgtccggt
     2641 cagcgtgacg gtgcgcggcc ccagatcgta aatgccgttg tcgcccgtcg cagtgcctga
     2701 cgacgaaaca aacaccacat tgccggtcgc ctcgaaacgg ttgggtttgc cttgcgcggc
     2761 gttgaccttc accctgtccg cgtgaagttt gtactcggcc tgggtcacga tcacgttgcc
     2821 gatataggtg cccaccttcg tctgaaagtc gcccacaaaa ttatcggagg agacgttgat
     2881 cggcgcgttg gcattgtgtt ggccgagatt gactgcgg
//
LOCUS       DASZMC010000324         7045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_951972,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000324 DASZMC010000000
VERSION     DASZMC010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951972"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VG274_06610"
     CDS             complement(<1..826)
                     /locus_tag="VG274_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06360.1"
                     /translation="MGHASMNAGTDAIAARSASHISRLWNLPLGDVRGEVAAIALLIP
                     LLLAAALWNGFPFIYYDTGAYVFEGLGGHFVVERSPVYSLFLRFAGARESLWLIVVIQ
                     AAATAFVMVQCAHAESPRLGIGPLLLIVALLVVGTALPWYVGQIEPDCFTALVALTIY
                     LLAFRARVLGRLHVIILVVIGGFAAAAHPSHLVLAGFLVLPIAAIRVLAAIARGWPKP
                     RLLESSAVFVLGLALVVGANFHFTRQIFVSRAGTSFVFARMLQDGIVMRLLDDTCPQ"
     gene            891..1307
                     /gene="sdhC"
                     /locus_tag="VG274_06615"
     CDS             891..1307
                     /gene="sdhC"
                     /locus_tag="VG274_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWE06361.1"
                     /translation="MAEQAKHATGARPLSPHLQIYRWPVTMATSIIHRVTGLALAAGT
                     LVVAWWLIAIATGPHSYNLFSMVARSIGGQTVLFGFVWSLAFHLFNGLRHLAWDSGFG
                     FEVRTANRTGVGAVALSLLAAIAVFAYAYLEKGVYI"
     gene            1304..1678
                     /gene="sdhD"
                     /locus_tag="VG274_06620"
     CDS             1304..1678
                     /gene="sdhD"
                     /locus_tag="VG274_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWE06362.1"
                     /translation="MSAQTPIARVEGLGASHSGTGHFWRQRVTALALVPLSIWFVCMA
                     LALIGAHRHEAIAFLSVPVNAILMALFIITALHHMVLGVQVVIEDYVHAEAPKIALLV
                     INQFFAWLVGAACLFALVKIAL"
     gene            1680..3476
                     /gene="sdhA"
                     /locus_tag="VG274_06625"
     CDS             1680..3476
                     /gene="sdhA"
                     /locus_tag="VG274_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWE06363.1"
                     /translation="MPTSPAYPITDHTFDVVVVGAGGAGLRATLEAAARGLRTACVTK
                     VFPTRSHTVAAQGGISAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIEYMCKNAPEAV
                     YELEHYGVPFSRTAEGKIYQRAFGGMTSHFGQGIAQRTAAAADRTGHAILHTLYGQCV
                     KHDVNFFIEYFALDLIVEDGAVRGLIAWNLDDGSMHRFRAHKVILATGGYGRIYFSCT
                     GAHTQTGDGNGMVLRAGLPLQDMEFVQFHPTGIYGVGVLITEGVRGEGGYMTNSEGER
                     FMERYAPNAKDLASRDVVSRAMTIEIREGRGVGPKEDHIDLHLSHLDPKIIHERLPGI
                     TESARIFAGVDVTKEPIPVLPTVHYNMGGIPTNHMGEALTLRDGNPDTVVPGLMAVGE
                     AACVSVHGANRLGSNSLIDLVVFGKAAGMRAAEAVTPGERHADLPKDAGEKSLARFDR
                     YRHAHGGTPTARIRLAMQMAMQEDAAVFRTGETLKKGRSRIDEIYRQLPDISVSDRSL
                     VWNSDLVETLELENLLQQAIITIEGAVMRTESRGAHAREDFPGRDDANWMKHTLAWLD
                     PATGWARLDYRPVHSYTLTNDVEYIPPKARTY"
     gene            complement(3479..6175)
                     /locus_tag="VG274_06630"
     CDS             complement(3479..6175)
                     /locus_tag="VG274_06630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HWE06364.1"
                     /translation="MHIRILTAAILIILASVAAPSVSSPVDPTTPTVGATAPDLVPMG
                     PLPRDVVPLRYRLAFTIDPSKPRFAGHDEIDVSFAKARRAIYLDGKALDVHRALLRLP
                     SGETIAARFAPVHDSGIALLSFAREVPAGGATLIFDYDANFGGALAGLYKVVDGANAY
                     AFTQFEATDARRAFPSFDEPGFKTPFEVSVTAPAGDNVVANTPVVSETATGDGLVRTL
                     FEPTAPLPTYLVALAVGPLDIVDGGDIPANSWRSLPIHLRGITAKGKGEQIRFALSFT
                     PRVVLALEDYFGIAYPFRKLDIIAVPDFAAGAMENAGAITFRERLLLMDGRAPLEQRR
                     RGLVVQAHEITHQWFGDLVTPHWWDDTWLNESFATWMEYKIAAATRGDWEFSRETLRA
                     GLAVMDLDELPSARSIHQPVNGPGDIENSFDAITYNKGAAVLSMFETYLGEDVFRAGV
                     SAYLKKFAFGSATARDFIGSLAEATNHPEVAAGFEDFIDRPGIPDLATRRICNASGNR
                     LRVSQSMYVPIGRAPASRIWRVPMCVAAPGQTKLCKLVDRSSATLPLGRPCPAYVFPN
                     AGGSGYFRFAAAEPDWRALIAAAPQLDAAEQLTLFSNISAALAAGQASAADFLALVAA
                     VAPNAKWDLAAAIHDSLHNFDQQILQSRDRPALRSFVRVHFAPRLAGMAYRGARLSEP
                     AGLALLRASLAALLVEESRDKQVTSALIPAGAAYLASDGLDTRGMSRDLMQEALRAGV
                     LEGGATFEDALVTAFLKSGKEYVRQQALIAAGATENSSYAERWIALMPQMRSGEVITL
                     LRLGSNDAVMRGRIWAWLKANYESLAVRLPPDAMAEMPGAMARACDKASRADLDSFFR
                     PKLSIVPGAARPLALAEEQIDRCIAFKTAKGAEIAAAFRRAK"
     gene            complement(6321..>7045)
                     /locus_tag="VG274_06635"
     CDS             complement(6321..>7045)
                     /locus_tag="VG274_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWE06365.1"
                     /translation="RPDVLNALHPPAHRELAEVFDGFAEDADQWVAIITGAGERAFSA
                     GDDLKYRAAGGAMSMPDSGFGGVARRFDLDKPLIAAVNGIAMGGGFEIALACDVMIAA
                     ESAVFALPEPRVGVAALAGGLHRLPREIGVKQALGMILTGKRVSARDGLALGFVNEVV
                     PAGEVLQAARRWAAQILDLSPMSVRASKQAVYRGLAEASVRDALANQGEYPAVAAMFR
                     SDDFAEGPRAFAEKRPPRWQGR"
BASE COUNT         1383 a         2241 c         2199 g         1222 t
ORIGIN      
        1 actgtgggca ggtgtcgtcc agcagacgca tgacgatgcc atcctgcagc atgcgcgcga
       61 acacgaagga agtccctgcc cggctgacga aaatctgccg cgtgaaatga aaattggctc
      121 cgacgacgag cgcgaggccg agaacgaaaa cagccgagga ctccagcagc cgcggcttcg
      181 gccacccgcg ggcgattgcg gccaatacgc ggatcgcggc aatcggaaga acgagaaatc
      241 ctgcgagaac gagatgcgag ggatgcgcgg ccgcagcgaa gccgccgatt acgacgagaa
      301 tgattacatg cagccgcccc aggactctcg cacgaaacgc gagcagatag atcgtgagag
      361 cgaccagtgc ggtgaaacag tcgggctcga tctggcccac ataccaggga agagcggtcc
      421 cgaccacgag cagagcgacg atcaatagca gcgggccgat cccaaggcgt ggcgattccg
      481 cgtgcgcgca ttgcaccatc acaaatgccg tggctgcggc ctggatcacg acgatgagcc
      541 aaaggctttc tcttgcgcct gcaaatcgca ggaacagcga gtaaacgggc gaacgttcga
      601 cgacgaaatg gccgccgaga ccttcaaaga cataggcgcc ggtgtcgtaa tagatgaacg
      661 gaaatccgtt ccagagtgct gccgcgagca ggagcggtat cagcagcgca atcgcggcaa
      721 cctcgccgcg aacatcgcca agcgggaggt tccataggcg ggaaatgtgg ctcgcactgc
      781 gcgccgcaat cgcgtccgtg cccgcattca ttgaagcatg ccccatgcgg ttgacttgac
      841 ccgactcacc cctaaactcc gcgccggctc gacattttgg tgatccttcc atggcggaac
      901 aggccaagca cgcgaccggc gcgcgtcctc tctcgcccca tctgcagatt tatcgctggc
      961 ccgtgacgat ggccacatcc atcatacacc gggtcacggg gctcgcactc gccgcgggta
     1021 cgctggttgt cgcctggtgg ctgatcgcca tcgcgaccgg acctcattcc tacaatctct
     1081 tctcgatggt ggcgcgcagt atcggcggac agaccgtgct gttcgggttt gtgtggtcgc
     1141 tcgcctttca tcttttcaat ggcctgcgtc acctggcttg ggattccggc tttggtttcg
     1201 aggtccgcac cgcgaaccgc accggcgtcg gcgccgtcgc actctcgctg ctcgccgcca
     1261 ttgcggtgtt cgcctacgcc tatctggaaa aaggcgtgta catttgagcg cgcagacacc
     1321 gattgcgcgt gtcgaaggtc tgggtgcttc gcattctggc accggtcatt tctggcggca
     1381 gagggtcacc gccctggcgc ttgtgccgct ctcgatctgg ttcgtctgca tggcgcttgc
     1441 cctgatcggc gcccatcgtc acgaggcgat cgcatttctt tccgtaccgg taaacgcgat
     1501 tctcatggcg ctcttcatca tcaccgcgct ccatcacatg gttctgggcg tccaggtcgt
     1561 gatcgaggac tacgtacatg ccgaggcacc caaaatcgcg ctgctcgtga tcaatcagtt
     1621 cttcgcctgg ctcgtcggcg ccgcctgcct gttcgcgcta gtgaaaattg ccctttaaaa
     1681 tgccgacctc gcccgcctat ccgatcaccg atcacacctt cgacgtcgtt gtcgtaggcg
     1741 ccggcggcgc gggactgcgc gccacgctcg aagcagcggc acgaggactt cgcactgcct
     1801 gcgtcaccaa ggtgttcccc acgcgaagtc atacggtcgc ggcgcagggc ggcatctcgg
     1861 ccgcgctcgg caacatgggt gaagacgact ggcgctggca catgtacgac acggtgaagg
     1921 gatcggactg gctgggagac caggacgcga tcgaatacat gtgcaagaac gcgccggaag
     1981 ccgtgtacga gctggagcat tacggtgtgc cgttcagccg cacggcggaa ggcaaaatct
     2041 atcagcgcgc cttcggaggc atgacctcgc attttgggca aggcatcgcg cagcgcaccg
     2101 ccgcggcggc cgaccgcacg ggccatgcca ttttgcacac gctttacggc caatgcgtga
     2161 agcacgacgt caacttcttc atcgagtact tcgccctcga tctgatcgtg gaagacggcg
     2221 cagtgcgcgg cctgatcgcg tggaatctcg acgacggctc gatgcaccga ttccgcgcgc
     2281 acaaagtcat tctcgccacc ggcggctatg ggaggatcta tttctcctgc acgggagcgc
     2341 acacccagac gggagatggc aacgggatgg tgctccgcgc cggactgccg cttcaggaca
     2401 tggagttcgt ccagttccac ccgacgggca tttacggcgt cggcgttctc atcaccgaag
     2461 gcgttcgcgg cgagggcggc tatatgacca attcggaagg cgagcgcttc atggagcgct
     2521 acgcgccgaa tgcgaaagat cttgcctcac gcgacgtcgt cagccgtgcg atgaccatcg
     2581 agattcgcga gggacgcggc gtgggaccga aagaggacca tatcgacctg catctctcgc
     2641 atctcgatcc gaagatcatt catgagcggc tgccgggcat caccgaatcc gctcgcatct
     2701 tcgccggcgt ggacgtgaca aaggagccga ttccggtgct gccgaccgtc cattacaaca
     2761 tgggcggcat tcccacgaac cacatgggcg aagcgctcac gctccgcgac ggcaatcccg
     2821 acacggtcgt gcctggcctg atggcggtcg gcgaagcggc gtgcgtatcc gtccacggcg
     2881 cgaaccggct cggctccaat tcgctgatcg atctcgtcgt attcggcaag gcggccggca
     2941 tgcgcgcggc ggaggcggtc acgccgggcg agcggcacgc cgacctgccc aaggatgccg
     3001 gcgaaaagtc gcttgcgcgc ttcgatcgct accgccatgc ccatggaggc acacccaccg
     3061 cgcggattcg tctggcgatg cagatggcga tgcaggagga cgcagcggtg ttccgcaccg
     3121 gcgagacgct gaagaaaggc cggtcccgca tcgacgaaat ctacaggcag ctgccggata
     3181 tcagcgtatc cgaccgctcg ctcgtctgga acagcgatct cgtcgagacg ctcgagcttg
     3241 agaaccttct ccagcaggcg atcatcacca tcgaaggtgc ggtcatgcgc accgaaagcc
     3301 gcggcgcgca cgcaagggaa gatttccccg gacgcgacga cgcgaactgg atgaagcaca
     3361 cgctcgcctg gctcgatccc gcgacgggct gggcgcgtct cgactatcgc cccgtgcatt
     3421 cctacacgct gacgaatgat gtcgaataca ttcctccgaa agcgcggacg tattagtctt
     3481 acttcgcacg cctgaacgcc gcggcgatct ccgcaccctt tgccgtcttg aacgcgatac
     3541 agcggtcgat ctgttcttcg gccagtgcga gcgggcgcgc cgctccgggc actattgaaa
     3601 gcttcggacg gaagaaggaa tcgaggtcgg cacgcgaggc tttgtcgcac gcgcgcgcca
     3661 tggcgccggg catctcggcc atcgcgtcgg gcggcagccg gacggccaag ctttcgtagt
     3721 ttgccttcaa ccatgcccag atgcgtccgc gcataaccgc gtcattcgaa cccaaacgga
     3781 gcaaggtgat gacctcgccc gagcgcattt gcggcataag cgcgatccac cgctcggcat
     3841 aggaagaatt ctccgttgcg cccgccgcga tcagcgcctg ctggcgaaca tattcctttc
     3901 cggatttgag gaacgcggtg acaagcgcgt cctcaaaggt cgcgccgcct tccagcacgc
     3961 cggcgcgcaa cgcttcctgc atcaggtcgc gcgacattcc tctggtgtcc agaccgtccg
     4021 aggcaaggta agcggcccca gccggaatga gagcggatgt cacttgcttg tcgcgggatt
     4081 cctcgaccag cagggcggcg agcgacgcgc gcaagagcgc aagccccgcc ggttccgaca
     4141 ggcgcgcgcc ccgataggcc atgccggcaa ggcgcggcgc aaaatgcacg cgcacgaatg
     4201 atcgcagcgc cggacgatct cgggactgga ggatttgttg gtcgaaattg tgcagcgaat
     4261 cgtgaatggc ggcggcgagg tcccatttcg cgttcggcgc cactgcggcc accagggcaa
     4321 gaaaatcggc ggccgacgcc tgccctgctg cgagggcggc ggaaatattg gaaaacaatg
     4381 tgagctgctc ggccgcgtcc agctgcggcg ctgctgcgat caatgcgcgc caatccggtt
     4441 cggcggccgc gaatcggaaa tagccgctgc cgcctgcgtt tggaaacaca taggcagggc
     4501 acggcctgcc gagcggaagc gtcgccgagg acctatcgac cagcttgcac aattttgtct
     4561 gacccggggc ggcaacgcac atcggcacac gccaaatccg cgaggcgggt gctcgtccga
     4621 tcggcacata catcgattgc gacaccctca gacggttgcc cgacgcattg caaatgcgcc
     4681 tcgttgcgag atcgggaatt cccggacgat cgatgaagtc ttcgaatccg gcggcgactt
     4741 cggggtgatt cgtggcttct gccagactgc cgatgaaatc acgtgcagtc gcgctgccga
     4801 acgcaaattt tttcagataa gccgaaaccc cggcccggaa aacatcttcg cccaaatagg
     4861 tttcgaacat ggagagaacg gccgcgccct tgttgtaggt aatcgcgtcg aacgaattct
     4921 cgatgtcgcc ggggccattg acgggctggt gaatcgaccg cgcggaaggc agctcgtcga
     4981 gatccatcac cgcaaggccg gcgcgcaggg tctcgcggct gaactcccaa tcccctctgg
     5041 ttgctgccgc aattttgtat tccatccagg tcgcaaagga ttcgttcaac catgtgtcgt
     5101 cccaccagtg cggcgttacc agatcgccga accattgatg ggtgatctcg tgcgcctgca
     5161 ccacgagccc cctgcgcctc tgctcgaggg gcgcgcgccc atccatgagc agcagccgct
     5221 cgcggaatgt gatcgctccc gcattctcca ttgcgccggc cgcaaagtcg ggcaccgcaa
     5281 tgatgtcaag cttgcggaac ggataggcga tgccaaaata atcttcgagc gcgaggacga
     5341 cacgcggcgt gaacgacagc gcgaagcgaa tctgttcgcc cttgcctttt gcggtgatcc
     5401 cccgcagatg gatgggaaga gaacgccagg aatttgccgg aatgtctccg ccatcgacga
     5461 tgtccaaagg tcccacggcg agagcgacga ggtaggtggg aaggggcgcg gtcggttcga
     5521 agagcgtgcg cacgagaccg tcgcccgtcg cggtctctga aaccaccggc gtgttggcga
     5581 ccacattgtc tccggccgga gccgtgacgc tgacttcgaa gggcgtcttg aatccgggct
     5641 catcaaagga aggaaacgcg cgccttgcat ccgttgcttc gaattgcgtg aaggcgtagg
     5701 cgttcgctcc gtcgaccacc ttgtacaaac cggcgagcgc gccgccgaaa tttgcgtcat
     5761 agtcgaagat cagagttgcg cctcccgcag gcacctcacg agcaaacgag agcaacgcga
     5821 tgcccgaatc gtgcaccggc gcaaatcgcg cggcgatcgt ctcgccggat ggcaggcgca
     5881 gcagcgcgcg gtggacgtcg agagcctttc catcgagata aatcgcacgg cgtgccttgg
     5941 cgaacgacac gtcgatttcg tcgtggcctg cgaagcgcgg cttcgatgga tcgatggtga
     6001 aggcaaggcg atagcgcagc ggaacgacat cgcggggcag cggtcccatt ggaaccaaat
     6061 cgggcgccgt cgcgccaacg gtcggagttg tcggatcaac gggactcgac acgctcggcg
     6121 ccgccacgct cgcaagtata atgagtattg cagccgtcag aatccggatg tgcatgcaat
     6181 tgcttccttg gagagcgatg cgatcggatt gaattgtttg cgctcgctga tacacataac
     6241 cgcccccttg tgaggaggta agctcaacgc gacaaccacc gaatgcagag gcagccgcgt
     6301 caatgtgatc gcaaccacct ttatcgtccc tgccagcggg gcggacgctt ttcggcgaag
     6361 gcgcgcgggc cctcggcaaa atcatcgctg cggaacatgg cggcgacagc gggatattcg
     6421 ccctgattgg cgagggcgtc gcgaacggac gcctctgcga gcccgcgata gaccgcctgc
     6481 ttggaggcgc gcaccgacat cgggctgagg tcgaggatct gcgccgccca gcggcgcgcc
     6541 gcctgcagaa cctcaccggc cggaaccacc tcattgacga agccgagcgc cagcccgtcg
     6601 cgcgcgctca cccgctttcc ggtgaggatc atgccgagcg cctgcttgac gccgatctcg
     6661 cggggcagtc tgtggagtcc tcccgccagg gcagcgacgc caacacgagg ttccgggagc
     6721 gcaaataccg ccgattccgc tgcaatcatg acgtcgcagg caagcgcaat ctcgaagccg
     6781 ccgcccatgg caattccgtt gaccgcggcg atcaagggct tgtcgagatc gaacctgcgc
     6841 gcgacgccgc cgaagccgct gtccggcatc gacatcgcac cgccagccgc ccggtactta
     6901 aggtcgtctc cggcgctgaa cgcccgctct ccggcgcccg tgatgatggc cacccactgg
     6961 tcggcgtctt ccgcgaagcc atcgaacact tcggcgagtt cgcgatgggc cggcggatga
     7021 agcgcgttga gcacatctgg ccggt
//
LOCUS       DASZMC010000325         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2886577,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000325 DASZMC010000000
VERSION     DASZMC010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2886577"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VG274_06640"
     CDS             complement(<1..739)
                     /locus_tag="VG274_06640"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HWE06366.1"
                     /translation="MTVIPFDDRDGSLWYDGRLVPWREAKTHVLTHGLHYASSVFEGE
                     RLYAGQIYKLAEHTARLFESARLLGMEIPYTEKEIRDACIEAGRVQGLDDGYVRPVVF
                     RGSEMMSVAAPKSKIHIAIAAWPWPSYFSPEEKSKGIRLMVSEWRRPGPDMAPTKSKA
                     AGLYMICTLSKHAAEAQGYADALMYDYRGLVAEATGANVFFVKDGALHTPIPDCFLDG
                     ITRRSVMALARARQIQVVERYIKPEEMS"
     gene            892..1590
                     /locus_tag="VG274_06645"
     CDS             892..1590
                     /locus_tag="VG274_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE06367.1"
                     /translation="MNSAAGDSHLLVVDDDVRLRALLQRYLSSNGYRVSAAADAAEAR
                     ALMKSMAFDLMIVDVMMPGESGLDLTKAVRAHSHTPVLMLTARGEPEDRIAGLERGAD
                     DYLAKPFEPRELLLRCAALLRRTTVPSRSPHREVKMGEALFDPERGELRRKGRPVRLT
                     SSESALLKLFAANAGRPFSRMDLCARLGVALERSIDVQVTRLRRKIEDDPKLPLYIQT
                     VRGVGYVLVPDRVD"
     gene            1597..2937
                     /locus_tag="VG274_06650"
     CDS             1597..2937
                     /locus_tag="VG274_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWE06368.1"
                     /translation="MAGLRPFRLLKRLSPRGLFGRSLIIIVAPMVVLQGIVTYAFFER
                     HYDIVTRHMAHSVAGDVAFLTTLAETYPAGGERTKLLAMAAHALGFGISLQPGARLDA
                     PYRHSSVDLKNAVNAVFANRLGAAHPFASNANGNVVDLSVQARDGVLKIEIPRDQVVA
                     SNADIFILWMVGSSLVLIAVAILFLRNQVKPIERLALAADAFGKGRAVPDFKPYGASE
                     VRKAAQAFLTMRARIDRYVQQRTEMLAGVSHDLKTPLTRLRLQLAMMGPGVDTASLIQ
                     DVSEMEHMLDEYLDFARGDSGEEAQAADLGEVVTEAALAAASARAAGGDRLHVATQSG
                     LRLAIKRNALKRCATNLIDNALKHGERVDVAIARNGHFVEILVDDDGPGIPESQREEA
                     FRPFHRLDEGRNLQTGGVGLGLAIARDIARAHGGDLTHGASKLGGLRAAIRLPV"
BASE COUNT          503 a          972 c          949 g          534 t
ORIGIN      
        1 ccgacatttc ttccggtttg atatagcgct cgacgacctg tatctggcgc gcccgggcga
       61 gcgccatgac ggagcggcgc gtaatgccgt ccaggaagca gtccggaatc ggggtgtgca
      121 aggcgccatc cttgacgaag aacacattgg cgcccgtcgc ctcggcgacc aggccgcgat
      181 agtcgtacat cagcgcatct gcgtagccct gcgcttccgc cgcgtgcttg gacagcgtgc
      241 agatcatgta gagacctgcg gccttcgact tcgtcggcgc catatcgggt ccgggacgcc
      301 gccattccga aaccatcagc cggattccct tgctcttctc ctccggactg aaataggacg
      361 gccagggcca ggcggcgatg gcgatatgga ttttcgattt gggcgcggca accgacatca
      421 tctcgctgcc gcggaagacc acgggcctca catatccatc gtcgaggccc tgcacgcgcc
      481 cggcctcgat gcaggcgtcc cggatctctt tctccgtgta cggaatttcc atgccgagaa
      541 ggcgcgccga ttcgaacagg cgcgcagtgt gctcggcaag cttgtagatc tggccggcat
      601 agagacgctc cccctcgaag accgaagagg cgtaatgcag tccatgcgtc agcacatggg
      661 tcttcgcctc gcgccacggc acgagacggc cgtcgtacca gagcgaaccg tcgcggtcgt
      721 cgaagggaat gactgtcatg cggagacccc gtgcggtttg cacttgtttc gaagccgccg
      781 gagggacaaa ttcggcacca tgtccagggc acggttcgcc gacggcgggt cgggacggcc
      841 ggtcccggta tttgatttat atgtgcgccc gggcgcatct aagcaagcga aatgaattcc
      901 gccgccggag attcccatct gctggtcgtc gatgacgacg tgcggctgcg tgcccttttg
      961 cagcgttacc tcagttcgaa cggctatcgc gtgagcgccg cagccgatgc cgccgaggcc
     1021 cgcgcgctga tgaagagcat ggccttcgat ctcatgatcg tcgacgtgat gatgccgggc
     1081 gagtccggcc tcgatctgac aaaggcggtg cgcgcccact cccacacgcc ggtcctgatg
     1141 ctgaccgcgc gcggcgagcc ggaggaccgc attgccggcc tcgagcgggg cgccgacgat
     1201 taccttgcca agcccttcga gccgcgcgaa ctgctgctgc gctgcgccgc cctgctgcgc
     1261 cgcacgacgg tgccctcgcg ctcgcctcac cgcgaagtga aaatgggaga ggcgctgttc
     1321 gatcccgaac ggggagagct ccggcgcaag ggcaggcccg tgcgcctcac cagttccgaa
     1381 tccgcgctgc tcaagctctt tgccgccaat gccgggcgtc cgttttcgcg catggatctc
     1441 tgcgcccgac tgggcgtcgc tctcgaacgg tccatcgacg ttcaggtcac gcggttgcgg
     1501 cgaaaaatcg aggacgaccc aaagctgccg ctttatattc aaaccgttcg cggcgtgggc
     1561 tacgtgctcg tgcccgatcg tgtggactga tcccagatgg cgggtttgcg gccgttccgg
     1621 cttctcaaga gattgtcgcc gcgcgggctg tttggccgct cgctgatcat catcgtcgcg
     1681 ccgatggtcg tgctgcaggg catcgtgacc tatgcgtttt tcgagcggca ctacgacatt
     1741 gttacccgcc acatggcgca cagcgtggcc ggagacgtgg cgttcctgac cacgctcgcc
     1801 gaaacctatc ctgcgggcgg cgagcggaca aaacttctcg ccatggcggc ccatgcgctc
     1861 ggattcggga tttcgctcca gccgggcgcc cggctcgatg cgccttatcg gcattcgagt
     1921 gtcgatctga agaatgcggt gaacgccgtt tttgccaacc ggctgggcgc ggcccacccg
     1981 ttcgcctcga atgcgaacgg caacgttgtc gatctgagcg tgcaggcacg ggacggcgtg
     2041 ctgaaaatcg aaataccgcg cgaccaggtg gtggcgtcca atgccgacat cttcatcctg
     2101 tggatggtcg gctcctcgct tgtgcttatt gcagtggcga tcctcttcct gcgcaaccag
     2161 gtcaagccga tcgaacggct cgcccttgca gccgacgcat tcggcaaggg ccgcgccgtg
     2221 ccggacttca agccctatgg ggcttcggaa gtgcgcaagg cagcgcaggc ttttctcacc
     2281 atgcgcgcgc gcatcgaccg ctacgtgcag cagcgcaccg aaatgctcgc cggcgtgagc
     2341 cacgacctca agacaccact gacgcgcctg cgccttcagc tcgcgatgat gggaccgggc
     2401 gtcgacaccg cgtccctgat ccaggatgtg agcgagatgg agcacatgct cgacgagtat
     2461 ctcgatttcg cgcgcggcga ttcgggggaa gaggcccaag ccgccgatct gggcgaggtg
     2521 gtgaccgagg cggcgctcgc cgcggcgagc gcgcgcgcgg cgggcggcga ccgtctgcac
     2581 gtcgccacgc aatcgggact ccgtctcgcc atcaaacgca acgcgctcaa gcgctgcgcc
     2641 accaatctga tcgacaacgc gctgaagcac ggagagcgcg tggatgtcgc gatcgcccgc
     2701 aacggccatt tcgtcgagat tctcgtcgat gacgacggcc cggggatccc cgaatcccag
     2761 cgcgaggaag cgtttcgccc atttcaccgc ctcgacgaag ggcgcaacct gcagacaggc
     2821 ggcgtcgggc tcggcctcgc gatcgcgcgc gacatcgccc gcgcccatgg cggagacctg
     2881 acgcacggtg caagcaagct cggcggattg cgcgcggcga ttcgcctgcc ggtgtgattc
     2941 ccttcatgtg ctgcgtgc
//
LOCUS       DASZMC010000326         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2611781,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000326 DASZMC010000000
VERSION     DASZMC010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611781"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..567)
                     /locus_tag="VG274_06655"
     CDS             complement(43..567)
                     /locus_tag="VG274_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HWE06369.1"
                     /translation="MPIAAFSNADDGLEPARRRRGHFVADTNLNDDGPWVPYAEGVWL
                     QPTCFNVTSGGFSLVLKGLPGAKLGVHYHVGTVQGYTLRGRWRYLEHDWIAGPGTFIY
                     EPAGEAHTLVIMDDSPEPALIFFAIEGALVYLDKPVSGAFLAYEDAFSALELTRKHYR
                     EAGLDARRLDPLIR"
     gene            838..1539
                     /locus_tag="VG274_06660"
     CDS             838..1539
                     /locus_tag="VG274_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWE06370.1"
                     /translation="MSDYDLYYWSVPFRGQFVRAVLAYAGKTWTEAGDAAISKLMGGP
                     VKHMPVPFMGPPLLIDKKTKFAIAEMPAIVFYLGETLGLMPAAPGSRAMTMKIVNDAN
                     DVLDEITLDGGRQMWTQKRWRDFVPRLKKWMSLWEETGRRHGLKADRGFLLGGKTPGI
                     ADIVTATLWSTMADRFPAIAAILEDAAPITAGLMRRVAHLPPLAKLAAKARKDYGDAW
                     CGGEIEASLRKVLEA"
     gene            complement(1661..2065)
                     /locus_tag="VG274_06665"
     CDS             complement(1661..2065)
                     /locus_tag="VG274_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HWE06371.1"
                     /translation="MHVMEMVGKHPGFKGAANVALIHCVEECYNCAQTCASCADASLA
                     EDDVKMLARCVRLNLDCTDMCVATGAIATRHLVSESPMLREILAACELTCRRCEEECL
                     KHASHHAHCRVCAEVCANCRKACEAAIASIGA"
     gene            2574..>4368
                     /locus_tag="VG274_06670"
     CDS             2574..>4368
                     /locus_tag="VG274_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWE06372.1"
                     /translation="MTELTIAPRGPSSPTWTSSAKDMVTTALGASRVWVTIGYGIVNE
                     IYWPATGTPQIRDLGFIVFGPKGWHEVKRVARYTIASPGPHIPLPQIVHEHPDYRLEL
                     EVAPDPDRDVVLISFRLHGDNLRLYALLAPHLGNSGVHNNGCAGRELYAWSGLPTLCL
                     ASDCGFSRSSAGHVGVSDGWQDFAQNGRMTWTYNAALDGNVALMGELAANEGTLALGF
                     SASQEGARTLARSSLSVGFARVRECFVEDWQEWGKMLKVPATTPAVEREAHISAAVLK
                     VHEDRTFPGSIVASLSVPWGNSSDSSGGYHLVWPRDCVKSGFALIAAGQIEDARRMLS
                     WLVATQKPDGTWSQNSFPDGRPFWTGIQLDEVAFPVLLAARLREENALAGIGGVANMI
                     FRAAGYIAANGPITSQDRWEENAGLSPFTIAVVIAALIAASEFVDHESANYLHSLVDC
                     WNERIEEWTYVERGPLAKIHGVDGYYIRIGPSAAEGGLQGSIELRNRAGVRVPAAALV
                     GMEFLALVRIGLRDANDPRILNTIAVADALLKVETPCGTAYRRYNGDGYGEQADGSPF
                     NGTGIGRAWPLLTAERGEYELMLGHDPLPWLE"
BASE COUNT          807 a         1358 c         1391 g          812 t
ORIGIN      
        1 cgacttgcga tggcagatcg ttgctgaaga gacatcaatc cgctagcgaa tgagcgggtc
       61 gagacggcgc gcatccaggc cggcctcgcg ataatgtttc cgcgtcagct cgagcgcact
      121 gaaagcgtct tcgtatgcga gaaaggcgcc gctcaccggc ttgtccagat aaacgagcgc
      181 gccttcgatc gcgaagaaga tcaacgccgg ctccggcgag tcgtccatga tcacaagagt
      241 gtgggcttcg ccggcgggct catagatgaa cgttcccggc cccgcgatcc agtcatgctc
      301 caggtatcgc caacgcccgc gcagcgtgta tccctggacg gtcccgacat ggtagtggac
      361 gccgagtttg gcgccgggca gtcctttgag gacaaggctg aatccgcccg aagtcacgtt
      421 gaagcaagtt ggctggagcc agacaccttc cgcataggga acccacggtc cgtcgtcatt
      481 caaattcgta tcggcgacga aatgcccgcg cctccgtcgc gccggttcga gcccatcgtc
      541 ggcgttgctg aatgcggcaa tcggcatctc agactctccc gtttgcggat cggacgatca
      601 ttggagcccg gcacgatagc ggaagaaact ggcgtcagat aatgcactac ctgcggctca
      661 acatcccggt ggcgagggcg gagcggttgc tgccccctcc accgcttcgc ggtccccctc
      721 ccccgcgcaa gcgcggggga ggaaaaaagg gggcgcgcgc gcaatgcatc gacacaatcg
      781 cgcgccgcca ggccccgcgc ggcgttatgg acagacatcg cccctggaac cctcaccatg
      841 tccgattacg atctctacta ctggtccgtg ccgttccgcg gccagttcgt gcgcgcggtc
      901 ctggcttatg ccgggaagac ctggaccgag gcgggcgacg ctgcgatctc gaagctgatg
      961 ggcgggccgg tgaagcacat gccggtcccg ttcatggggc cgccgctgct gatcgacaaa
     1021 aagacgaagt tcgcgatcgc cgagatgccg gccatcgttt tctatctggg cgagacgctc
     1081 ggcctcatgc cggctgcccc tggctcacgc gccatgacga tgaagatcgt caacgacgcc
     1141 aacgacgtgc tcgatgagat cacgctcgat ggcggccgac agatgtggac ccaaaaacgc
     1201 tggcgcgatt tcgttccccg cctgaagaaa tggatgtcgc tctgggagga gaccggccgc
     1261 cgccacggac tgaaagcgga ccggggcttc ctgctgggcg gcaagacgcc tggcatcgcc
     1321 gatatcgtga cggcaaccct gtggtcgacc atggccgacc gcttccccgc aatcgcggcg
     1381 atcctcgaag acgctgcgcc catcacagcc ggtttgatgc ggcgggtcgc gcatctgccg
     1441 ccgctggcaa aactggccgc aaaggcgcgc aaggactatg gcgatgcctg gtgtggcggc
     1501 gagatcgagg cttcgctgcg aaaggttctg gaagcctgac tcccgacact gattcaattt
     1561 ctttggtcga cggtgcgttg gcgcacgata ccggatgaaa cggcgaaccg ggcggccgtc
     1621 ggcataaggc gactgagccc cgcggctgcg atagcaaagc ctatgcaccg atcgaagcga
     1681 tggccgcctc gcacgccttg cggcagttcg cgcaaacctc ggcacaaacg cggcaatggg
     1741 cgtgatggct tgcgtgcttc aggcattcct cctcgcagcg ccggcacgtc agctcgcaag
     1801 ccgcgaggat ttcccgcagc atcgggcttt cgctcacgag gtgccgggtt gcgatcgcgc
     1861 cggtcgcaac gcacatatcg gtgcagtcga ggttgagccg gacacacctg gcgagcatct
     1921 tgacgtcgtc ctccgcgaga gacgcatcag cgcaggacgc gcaggtttgc gcgcaattat
     1981 agcattcttc gacgcaatga atcagcgcaa cgttcgctgc ccccttaaag cccggatgct
     2041 tgccgaccat ttccattaca tgcatggcgc gggatccctc catcttcgga acctggagcg
     2101 tagcgggtca gccggccaaa gcaacgcgtg cgattctcgt cgggcgccgg accccggtac
     2161 gactttcgag ggtgatgcct ggcacgaatc tccggtccga cattcttttt tccacgccat
     2221 atctcacccg atatccccga ggcttggggg cgcgatgccg agacgcgcgg gtgctggaat
     2281 cggcgccgcg atcgagttag ccacgctgcg agcagagtgg cgaccgcgcc gataattccg
     2341 ctgacgacgc tgctgaggag ggtaggcatg cggcggaggt ctggatatga acgtcgtggt
     2401 tttcattgtc ggtacggacc gattgaaaca caggctgcgc caatctcgat ggttcaggca
     2461 gccagtcctt tatgattcgc cggaagtgac atgcagaatg actgtccctg ggccttggtc
     2521 tcccccgtta acgaacagga tgggtgcata tattccgccc acacgagcga gaaatgaccg
     2581 aactgacaat agcgccgcgg gggccgagtt cacccacctg gacttccagc gccaaggaca
     2641 tggtgacgac ggcgctcggc gccagccgcg tctgggtgac gatcggctac gggattgtga
     2701 acgagatcta ctggccggcg accggcacgc cgcaaattcg cgacctgggt ttcattgtct
     2761 tcggcccgaa aggctggcac gaggtcaagc gggtcgcgcg ctacaccatt gcttcgccgg
     2821 gtcctcacat ccccctgccg cagatcgtgc acgaacatcc cgattaccgg ctggagctcg
     2881 aagtggcgcc cgatccggat cgcgatgtgg tgctgatttc atttcgcctt cacggcgata
     2941 atctgcgcct ctacgcattg ctcgcgccgc atctcggcaa cagcggcgtg cacaacaatg
     3001 gctgcgcggg ccgcgaactc tacgcctggt cgggcctccc cacgctgtgc ctcgcgagcg
     3061 attgcggttt ttcgcgttcg agcgcgggtc atgtcggcgt ctcggacggc tggcaggatt
     3121 tcgcgcagaa cggccggatg acctggactt acaatgctgc gctcgacggc aacgtcgcgt
     3181 tgatgggcga actcgcagcg aatgagggca cgcttgccct tggtttctcc gcgtcgcagg
     3241 aaggtgcacg cacgctcgcc cgctcgagcc tcagcgtcgg atttgcccgc gtccgcgagt
     3301 gtttcgtcga ggactggcag gagtggggaa aaatgctgaa agttccggcg acaacgcccg
     3361 ccgtggaacg ggaagcacat atttcggcgg ccgttctcaa ggtgcacgaa gatcgcacct
     3421 ttccgggctc gatcgtcgcc agtctcagcg tgccctgggg caattcgagc gacagctcgg
     3481 gaggttatca ccttgtgtgg ccgcgcgatt gcgtcaaatc cggctttgcg ctgatcgctg
     3541 cgggccagat cgaggatgcg cgccgcatgc tgtcgtggct tgtcgccacc cagaagccgg
     3601 atgggacgtg gtcgcagaac agctttcccg acgggcggcc gttctggacc ggcattcagc
     3661 ttgatgaagt cgcatttccg gttctgcttg ccgcaaggct gagagaagaa aatgcgctcg
     3721 ccggcattgg cggcgttgcg aacatgatct tccgggccgc tggctacatc gccgccaacg
     3781 gacccatcac cagccaggat cgctgggaag aaaatgcggg cctgagccct ttcaccatcg
     3841 ctgtggtgat cgcggccctc attgcggctt ccgaattcgt cgatcacgaa agcgcaaatt
     3901 acctgcattc gctcgtcgat tgctggaacg aacggatcga ggagtggacc tatgtggagc
     3961 gggggccgct cgccaaaatt catggcgtcg atggctatta cattcgcatc gggccttcgg
     4021 cggcggaagg cggccttcag ggctccatcg aattgcgcaa tcgcgccggc gtgcgggttc
     4081 ccgccgctgc gttggtcggg atggaattcc ttgcgctcgt ccggataggt ctcagggacg
     4141 cgaatgatcc gcgcatactg aacacgattg ccgtggcgga tgcgctgctc aaagtcgaga
     4201 cgccatgtgg cacggcttat cgccgctata acggcgacgg ctatggcgag caggccgatg
     4261 gcagcccctt caatggtacg ggcatcggcc gcgcctggcc gttgctcacg gccgagcgcg
     4321 gcgagtatga actcatgctg ggccacgacc cgcttccgtg gctggaaa
//
LOCUS       DASZMC010000327         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2340881,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000327 DASZMC010000000
VERSION     DASZMC010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340881"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..3611)
                     /locus_tag="VG274_06675"
     CDS             complement(9..3611)
                     /locus_tag="VG274_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWE06373.1"
                     /translation="MDHPVLIPDIRQGWRFYVDRGGTFTDVVAQAPDGGLASLKLLSE
                     SDRYDDAALEGMRRFLHVPTNADIPSELLDELRVGTTVATNALLERRGERVLLVTTRG
                     FRDQLRVGYQNRPKLFSLRIDPPQPLYERVIEADERIGAGGEVIRPLDEASLTRDLAS
                     ARAEGFAACAIVFMHGYRHSAHEQRAAEIARSLGFAQVSTSHETVPLIKYVSRGDTTA
                     ADAYLSPVLGRYSNRIAEKIGAGGSASRLFFMTSSGGLAAVSAFHGKDAVLSGPAGGV
                     VGMAETARAAGFDRVIGFDMGGTSTDVSRFDGVYERSQECEISGVRLRVPMMAIHTIA
                     AGGGSILHYDGARFRVGPDSAGANPGPMSYRRGGPLTVTDANLLVGKLRKDRFPQIFG
                     AQGDQALDEDCVRLAFGRLAQATNRTPEDVADGFIRIAVENMANAIKRISVARGHDVR
                     HYALACFGGAAGQHACLVADALGISRIFIHPFSGVLSAYGMRLASLRAFRHRAINNVL
                     DGGLAEQLAQSARALAKDAASDVEAQGGSNVATQATAHIRYAGSDTTLPIPLGQAEEM
                     THHFASTHARQFGFGFENRPLIVESIEIEAWSREAVGAAQQPPSTTAPHPAIGRARFY
                     SRHEWRDCDVVDTGGIASGATTDGPALLIEPHQTIVVEPGWRAEMSSARAVILTRTTP
                     RSELPHAATAADPVVLEVMANLFMAIAEEMGATLQSTASSVNIKERLDFSCAVFDADG
                     NLVANAPHMPVHLGSMGDCVASVIARYGSGMAEGDMFASNAPYDGGTHLPDVTVVAPV
                     FIGGERRFFVAARGHHADIGGISPGSMPPFSCNISEEGALFDAIRMVEGGRFDESAVR
                     AVLGAGEHPARNPDQNIADLKAQAAACARGNFELQRAAQLYGADAITAYMRHVQDNAE
                     EAVRKVIGALADGAFVLPMDGGEEIRVTITVDRERRCARVDFGGTSAQSDTNLNAPRS
                     VTKAAVLYVFRCLVGDDIPLNAGCLKPLDIVVPEGSLLNPRYPAAVAGGNVETSQAVV
                     DALFGALGVMAAAQGTMNNLTFGNANHQYYETICGGAGAGADFDGASAVHTHMTNSRL
                     TDPEILETRYPVLLEEFSIRRGSGGRGRHHGGDGVLRRIRFREDMTAAILSTRRETEP
                     FGLKGGEPGLPGANMMIRAGGETIALRGRDEIVVAPGDQILIATPGGGGFGRTE"
BASE COUNT          649 a         1252 c         1242 g          655 t
ORIGIN      
        1 cagaaatgtc actcagttct gccgaagccg ccgccgccgg gcgtggcgat caggatttgg
       61 tctccgggtg cgaccacgat ctcgtcgcgg cccctgagcg cgatcgtctc gccgccggcg
      121 cggatcatca tgttggcgcc cggcaggccc ggctcgccgc ccttcagccc gaacggttcg
      181 gtttcgcgcc gcgtcgacag gatcgccgcc gtcatgtctt cgcggaaccg gatgcgccgc
      241 agcacgccgt cgccgccatg atgccggccc cggccgccgg aaccgcgccg gatcgagaat
      301 tcctcaagca acaccggata gcgcgtctcc agaatctccg gatcggtcag acgcgaattg
      361 gtcatatggg tgtgcacggc cgacgcgccg tcgaaatcgg cgcccgcccc ggcgccgccg
      421 cagatcgttt cataatattg gtgattggcg ttgccgaagg tgagattgtt catggtgccc
      481 tgcgcagcgg ccatcacgcc cagcgcgccg aacaatgcgt cgacgacggc ctggctcgtc
      541 tccacattgc cgccggcgac ggcggccgga taacgcggat tgagcagcga gccttcgggc
      601 acgacgatgt cgagcggctt gaggcagccg gcgttcagcg gaatgtcgtc gccgacgagg
      661 cagcggaata cataaagcac ggcggctttc gtcaccgagc gcggcgcatt gagattggta
      721 tcgctctgcg cggaggtgcc cccgaagtcg acgcgcgcgc agcgccgctc gcgatcgacg
      781 gtgatcgtga cgcggatctc ttcgccgcca tccatcggca gcacgaacgc accgtcggcc
      841 agcgcgccga tgaccttccg caccgcttct tccgcattgt cctgcacgtg gcgcatatag
      901 gcggtgatgg catcggcgcc ataaagctgc gccgcgcgct gcagctcgaa attgccccgc
      961 gcgcacgccg ccgcctgggc tttgagatcg gcgatgttct ggtcgggatt gcgcgccggg
     1021 tgctcgcccg cgcccagaac cgcgcgcacc gcgctctcgt cgaagcggcc tccttcaacc
     1081 atgcggatgg cgtcgaagag cgctccctct tcgcttatat tgcaagagaa cggcggcatc
     1141 gagcccggcg aaatcccccc aatgtcggcg tgatgtccgc gcgcggcgac gaagaatctg
     1201 cgctcgccgc cgatgaagac aggggcaacc acggtgacgt cgggcagatg ggtgccgcca
     1261 tcgtagggcg cgttgctcgc gaacatgtcg ccctcggcca ttccactccc gtagcgcgcg
     1321 atgaccgacg cgacgcaatc gcccatcgat ccgagatgca ccggcatgtg cggagcattg
     1381 gcgacgaggt tgccatccgc gtcgaacacg gcgcaggaga aatcaagccg ctccttgatg
     1441 ttcaccgagg atgcggtgct ctgcagcgtg gcgcccattt cctcggcgat cgccatgaac
     1501 agattggcca tcacctcgag aaccacggga tcggcagcgg tggcggcatg cgggagctcg
     1561 gatcgcggcg ttgtgcgcgt gaggatcacc gcgcgcgccg aactcatttc cgcgcgccag
     1621 cccggctcga cgacgatcgt ctgatgcggt tcgatgagaa gcgccggccc gtcggttgtc
     1681 gcgccgctcg cgatgccgcc cgtatcgacg acatcgcaat cccgccattc gtgccgcgaa
     1741 tagaatcgcg cccggccgat tgcgggatgc ggagccgtcg ttgacggtgg ttgctgcgcg
     1801 gctccaaccg cttcccggct ccaagcctct atctcgatcg attccacgat gagcggccgg
     1861 ttttcgaagc cgaacccgaa ttgccgtgca tgcgttgacg caaagtggtg cgtcatctcc
     1921 tctgcctgtc cgagcggaat gggcagcgtc gtatcgctgc ccgcatagcg gatatgggcg
     1981 gtggcctgcg tcgcgacgtt gctgccgccc tgcgcctcga catcgcttgc cgcatccttc
     2041 gccagcgctc gcgcgctctg cgccagctgt tctgcgagcc cgccatcgag cacgttgttg
     2101 atcgcacgat ggcggaaggc cctgagcgat gcgagtctca tgccatatgc cgaaagcact
     2161 ccggagaacg gatggatgaa gatgcgcgaa atgcccagcg cgtcggcgac caggcaggca
     2221 tgctgtccgg ccgcgccgcc gaagcaggcg agcgcgtaat ggcgcacgtc atgaccgcgg
     2281 gcgaccgata tccgtttgat ggcattcgcc atgttttcga cggcgatgcg gatgaatccg
     2341 tcggccacgt cttcgggggt gcggttcgtc gcctgtgcga gacgtccgaa ggctaggcga
     2401 acgcagtcct cgtcgagcgc ctgatccccc tgcgcgccga atatttgtgg gaagcggtcc
     2461 ttgcgcagct tgccgaccag cagattggcg tcggtgacgg tgagcgggcc gccgcgccga
     2521 tagctcatcg gtcccggatt ggcgcccgcg gaatcgggcc cgacgcgaaa gcgtgcgccg
     2581 tcatagtgca gaatcgagcc gccgcccgcg gcaatcgtat ggatcgccat catcggtacg
     2641 cgcaaccgaa cgccggagat ctcgcattcc tggctgcgtt cgtagacgcc gtcgaagcgc
     2701 gacacgtccg tcgaggttcc gcccatgtcg aagccgatca cgcggtcgaa gccggcggcg
     2761 cgcgccgtct cggccatgcc gacgacgccg ccggccggtc ccgacagcac ggcatccttg
     2821 ccgtgaaagg cggacacggc cgccagtccg ccgctcgacg tcataaagaa gagccgcgac
     2881 gcgctcccgc ccgcgccgat cttttcggcg atgcggttgg aataccgtcc cagcacaggc
     2941 gacagatagg cgtcggcggc cgtcgtgtcg ccgcggctga catatttgat cagcggcacg
     3001 gtctcgtggc tggtcgagac ctgtgcaaat ccaaggcttc gtgcgatctc cgcggcgcgc
     3061 tgctcgtgtg cggaatggcg gtagccatgc atgaacacga tggcgcaggc cgcgaagccc
     3121 tccgcgcggg cggaagcaag atcgcgggtg agcgaggctt cgtcgagcgg acgaatgact
     3181 tcgcctccgg cgccgatccg ttcgtccgcc tcgatgacgc gttcgtacag cggctgcgga
     3241 ggatcgatgc gcagcgaaaa aagttttgga cggttctgat aaccgacgcg aagctggtcc
     3301 ctgaagcctc gcgtggtgac caggagaacg cgctcgcctc gccgctcgag gagcgcgttg
     3361 gttgccaccg tcgttccgac gcgaagctcg tccagcaatt cggaaggaat atcggcattg
     3421 gtcggcacgt ggaggaagcg gcgcatgcct tccagcgcgg catcgtcgta acgatcgctt
     3481 tccgacagca atttgaggct ggcgagtccg ccgtccggcg cctgcgccac cacgtcggtg
     3541 aacgtgccgc cgcgatcgac ataaaaccgc cagccctgac gaatatccgg aatcaaaact
     3601 gggtgatcca tttgcgtgac ccgccgaaag atataacgca gtggtcgcgg tggaagcgcg
     3661 ggggacgcag agggaactta tggcgcgcta cgcgcgcaag gaaacctcag cgaccattgc
     3721 gcaacctcag cgtcctccgc gttaaaacct tctctctccg ggcagattgg cttaatggtt
     3781 ttggtcatgt ccatctgg
//
LOCUS       DASZMC010000328         4583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4528748,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000328 DASZMC010000000
VERSION     DASZMC010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4528748"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..765)
                     /locus_tag="VG274_06680"
     CDS             complement(379..765)
                     /locus_tag="VG274_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE06374.1"
                     /translation="MRLLLDTHVLLWALIEPAKLGPDIHALLENPEHGILFSAASVWE
                     MAIRVALGRGDFGVPPETIVEAALASGFIELPVRSAAALKVASLPHDHRDPFDQLLVA
                     QAMTEPPALHTSDAQLEAYSGLVVLV"
     gene            complement(766..1008)
                     /locus_tag="VG274_06685"
     CDS             complement(766..1008)
                     /locus_tag="VG274_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWE06375.1"
                     /translation="MQTVNVHAAKTHFSRLVDQAAAGEEIIIAKAGKPVAKLVPLRSS
                     GKHAKRQLGLLAGRARIPAALDAPLPDGVLDTFEGR"
     gene            complement(1155..2576)
                     /locus_tag="VG274_06690"
     CDS             complement(1155..2576)
                     /locus_tag="VG274_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWE06376.1"
                     /translation="MVRYSFIAVDLHHLLLAGLPAHLTRLLTMRWLFVVTISAFTLAS
                     IGCASSGSFFALTAWRVLQGFSGGTLIPAVFSAVFLLFPHRLQGIATTIAGVLAVLAP
                     TVGPIVGGWITQTYSWHWLFLINVAPGILSAAVAARFLPMERPVPGEAGALDILSMGC
                     MAVALATLEIALKEAPRRGWLSPLVCGLLAVSVASGVAFARRSLGRRKPFVDLRTLKD
                     PAFAVGCLLSFVLGIGLFGSVYMMPVFLAFVRGHGALEIGGTMLVTGVAQLATAPAAV
                     ALERRMDARLLTAAGFAVFALGMEMSAFQTPKTDFAGMLWPQIVRGAAIMFCLLPPTR
                     LALGHLPPVAVPDASGLFNLMRNLGGAIGIALIDSVIYGRVPAHAQAIVHRLMAGDIE
                     TARFVGIPLQLFRNRPAGSLDPDTQSILEAMVNHAAFVRAMNDAWTLAAALTLAALFA
                     LPFARVRRKSPGGSDQCAKKIFR"
     gene            2867..4402
                     /gene="ltrA"
                     /locus_tag="VG274_06695"
     CDS             2867..4402
                     /gene="ltrA"
                     /locus_tag="VG274_06695"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWE06377.1"
                     /translation="MHDEEKSDSAIVAVKLVNKAEQSAAEPMEPRAEAEENAQQDGMR
                     RTPSREGMSHGLERIRTAARLDKKVRFTALLHHVGVDLLRNAYSQLRRDAASGVDGVT
                     WHDYGEGLEQRLKDLHARVHRGAYRVQPSRRKYIPKPDGRKRPLGIAALEDKLVQRAT
                     AEVLNAIYEEDFLGFSYGFRPKRSQHDALDALAVGIERTAVNWIVDADIAGFFDAVSH
                     EWLITFVEHRIGDQRVIRLIRKWLKAGMTEEDGRTMPTQIGTPQGAVISPLLANIYLH
                     YVFDLWAHQWRSRHARGNVIIVRYADDIVVGFEHEAEAKRFLAELRERMAQFALSLQP
                     EKTRLIEFGRHAASDRERRGQGKPESFAFLGFTHICAKSRRGAFQLRRKTRRDRMRAK
                     LAAIKQELRWRLHTSIPEQGTWLRHVVTGYFAYHAVPTNAPALSAFRYFVSQLWLRSL
                     KQRSQRSAVTWRLMAKLEAQWLPKPRILHPWPSQRFAVKHPRWEPYALIGLVRICAGG
                     AQQ"
BASE COUNT          936 a         1389 c         1505 g          753 t
ORIGIN      
        1 gcggcggcgc agcgctacat cgcgctcacg cccgcggatg tgcaggccgc gttcaagaaa
       61 tggatgcgcc cccccgacct cgtccgcgcc agccagggcc ccgtgccgga gtaagggccc
      121 ctcaccggcg cctgaacggt agatagtcct tgcgcttaag ccctctccca cgaggggaga
      181 ggggactccg tgcccttgtt ttcccgcgtt gatgcagcac gccgatttcc ctcccccctc
      241 gtgggagaag gatgccgatg tgtgtcatga attctgattt ggcgcggggt gaggggggaa
      301 cacaggggta cgtcgcacaa gcggaaagat catacattcc gcacggcggc cgagcgcccg
      361 cccgacgcga ggccacgctc agacaagcac tacaagcccg gaataggctt cgagttgcgc
      421 atcggacgta tggagcgccg ggggttcggt catggcctgc gccacaagaa gctgatcgaa
      481 gggatcgcga tgatcgtgcg gcagggaggc aactttcaac gctgccgccg agcgcacagg
      541 cagttcgatg aaaccgcttg ccagcgcggc ttccacaatg gtctcgggcg gcacgccgaa
      601 atctccccgc ccaagcgcca ccctgatcgc catttcccag acgctcgctg cgctgaacag
      661 aatcccgtgt tcaggatttt cgaggagagc gtgtatgtcc ggtcccagct tcgcgggttc
      721 gatcaacgcc cacagcagaa cgtgggtgtc gagcagcaat cgcacctatc ggccttcgaa
      781 cgtgtcgaga acgccatccg ggaggggagc gtccagcgcg gccggtatgc gcgcgcggcc
      841 cgcgagaaga ccaagttgcc gttttgcgtg ttttcccgac gaacgcaaag gcacgagttt
      901 ggcgaccggc tttcccgctt tggcgatgat gatttcctcg cctgccgctg cctggtcaac
      961 gagccgcgaa aagtgggttt ttgccgcgtg aacattcacg gtctgcatgc tggaacctcc
     1021 acatggacta accttagtcc atgctgtcgc aagtcacaag ggggactgag tcggagcggc
     1081 gaggggaaca cgggaataat tgggagacgg aaggatcata tacttcggat cggcgaccga
     1141 ccggctcgcg cggttcagcg aaatattttt ttggcgcatt ggtccgagcc gccagggctc
     1201 ttccgcctga cacgcgcgaa gggaagggca aaaagcgcgg ccagcgtgag tgccgctgcc
     1261 agtgtccacg catcgttcat ggcgcgaacg aaggcggcgt ggttgaccat cgcctccaga
     1321 atgctttgcg tgtcgggatc gagcgagcct gccgggcgat tgcggaacag ctggagggga
     1381 ataccgacaa atctggcggt ctcgatgtcg ccggccatga gccggtgcac gattgcctgc
     1441 gcgtgagcgg gtacgcggcc atatatgaca ctgtcgatga gcgcaatgcc gatcgcgccg
     1501 ccgagattgc gcatcagatt gaacagtccg ctggcatcgg gcacagcaac gggcgggaga
     1561 tggccaagcg ccagccgcgt cggcggcaac aggcagaaca tgatcgccgc gccgcgtacg
     1621 atctgcggcc agagcatgcc ggcgaagtcg gttttcggcg tctgaaatgc gctcatttcc
     1681 atgccgagcg cgaagactgc gaagcccgcc gcggtgagga gtctcgcatc catccttcgc
     1741 tcgagcgcga ccgcggccgg cgccgttgcg agctgggcga cgcctgtgac cagcatcgtg
     1801 ccaccgatct ccaacgcgcc atgaccgcgc acgaaggcga ggaacaccgg catcatgtag
     1861 accgagccga agaggccgat gccgagcacg aagctcagca ggcagccgac ggcgaaggcg
     1921 ggatccttga gcgtgcgaag atccacgaac ggtttgcgcc ggccgaggga tcgccgcgca
     1981 aacgccacgc cgctggccac cgaaaccgcg agaaggccgc acacaagtgg agaaagccaa
     2041 ccgcggcggg gtgcctcctt cagcgcaatc tccagcgtcg cgagcgccac cgccatgcaa
     2101 cccatcgaga ggatatccaa tgcgcccgct tcgccgggca caggcctttc catcggcaga
     2161 aaacgcgccg ccaccgccgc cgacaggatt cccggcgcga cgttgatcag gaacagccag
     2221 tgccacgaat aagtttgtgt gatccagccg ccgacgatgg gtccgaccgt gggcgcgagg
     2281 acggcgagga cgccggcaat cgtcgtggcg ataccctgca gacgatgagg gaacaaaaga
     2341 aacaccgccg agaaaacggc agggatcagc gttccgccgg agaagccctg aagtacgcgc
     2401 catgccgtca gcgcgaaaaa gcttccgctc gacgcgcagc cgatcgacgc aagcgtgaag
     2461 gcggaaatcg tcacgacaaa cagccaccgc atggtcagca gacgggtcag gtgcgccggg
     2521 agaccggcaa ggagcaggtg gtgcaagtcc actgcgatga aggagtagcg aaccacatcg
     2581 gccccgagcc gtgcgtgtct gtgccgcgag gcaaagcgcg aagcgtcggc aggggaacgt
     2641 ataggccagc cattgagccg cgaaagcttc gttcggagcg ccgacgccgt ctgaccagcg
     2701 gaaggcaaca cgatcgcatg cgtcaacgcg agcacgcgat cggctctgcg tggtctgaga
     2761 ccctggcatg tacgcacgct ctttgcacgg gaaccgggag gtctcttcgt tgaccgcttg
     2821 caagtgcacc ggtccgcgtc gggaaggcga tgagccgaag ccggcgatgc acgacgaaga
     2881 gaagtctgac tccgccatag tagctgtgaa gttggtgaac aaagcggagc aatccgctgc
     2941 ggagccgatg gagccaaggg cggaggccga ggagaacgcg caacaggacg gcatgcgccg
     3001 gacaccgagc cgggaaggca tgtcccacgg actggagcgc atacggacag ccgcaaggct
     3061 ggacaagaag gtgcggttca ccgcgctgct ccaccatgtc ggcgtcgatc ttctgcgcaa
     3121 tgcctattcc cagctcaggc gggatgcggc atcgggcgtg gacggcgtga cgtggcacga
     3181 ctacggcgaa ggtctcgaac aacgtctcaa ggaccttcac gcacgcgtgc atcggggcgc
     3241 atatcgggtg cagccatcgc ggcggaagta catacccaag ccggatggaa gaaaacgccc
     3301 gctcggcatc gcggcactgg aggacaagct cgtccagcgt gcgactgccg aggtgctgaa
     3361 tgcgatctac gaggaagact tcctcgggtt cagctatggg ttccggccga agcgcagcca
     3421 gcatgatgcg ctggatgcat tggcggtcgg gatcgaacgc acagcggtga actggatagt
     3481 ggacgccgac attgccgggt tcttcgatgc ggtcagtcat gaatggctga tcacgtttgt
     3541 ggagcaccgg atcggcgacc agcgcgtaat ccgcctgatc cgcaaatggc tcaaggccgg
     3601 gatgacggaa gaagacggac ggacgatgcc gacacagatc ggcacaccgc agggtgccgt
     3661 gatctcgccg ctattggcga atatctatct gcactacgtc ttcgacctct gggcgcatca
     3721 gtggcgctct cgccatgccc ggggcaacgt catcatcgtg cgctacgccg acgacattgt
     3781 cgtgggcttc gagcacgagg ccgaggccaa gcggttcctg gcggagctgc gcgaacggat
     3841 ggcgcagttt gcgctgagcc tgcaaccgga gaaaacccgg ctgatcgagt tcggacgcca
     3901 cgcggcgtcg gatcgcgaac ggcgtgggca gggaaagccg gagagctttg cattcctggg
     3961 gttcacgcac atctgtgcga aatcccgccg cggtgccttc caactcagac ggaagacacg
     4021 gcgggatcgc atgcgcgcca agctcgcggc gatcaagcag gaattgcgat ggcgcctcca
     4081 tacttccatt ccggaacagg gtacgtggct tcgtcatgtc gtgaccggct acttcgccta
     4141 tcacgccgtg ccgacaaatg ccccggctct gagtgcgttc agatacttcg tcagccaact
     4201 ctggcttcgc tcgctcaagc agcgcagtca gcgttcggcg gtcacgtggc gtctgatggc
     4261 aaagctcgaa gcgcagtggc tgcccaaacc acgcatcctt catccatggc cgagccagcg
     4321 cttcgccgtc aaacatccga ggtgggagcc gtatgcctta atcgggctcg tacggatctg
     4381 tgcggggggc gctcagcaat gagcgtccct accgcgaaag ttggaatcgc ttctctcatt
     4441 ccgcgttggg gaactggcgg gtagtgatgg agtacgtctg gcattcttgg gagccgttcg
     4501 cccacttttc ggcaacaatt ccgaaatcac tcatgagtgg ctgatttgtg gtgtaggcgt
     4561 tcacttgggt tcctgtggcc gtt
//
LOCUS       DASZMC010000329         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_128752,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000329 DASZMC010000000
VERSION     DASZMC010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128752"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1030
                     /locus_tag="VG274_06700"
     CDS             269..1030
                     /locus_tag="VG274_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06378.1"
                     /translation="MRSRSIILSGAATLVLATGGALAQSGYGDGRYGNGPAESTPAEM
                     QQTGQLNQQSVNGTTQSPAVLNGEQAPQNGPVNDEGAAPDTQSQPMTQTQYAQPQSQG
                     EYNAQQQQYQDQMQQYRDQRQRYRDDRDRYARNLRDWDLAQYAWYYPRPYVYRYGDEY
                     GLQPLYLIAEPSHQLAQLPVEGPGGRWVGRVRNVDIAPDGRPARIEIALNRRVSVWVR
                     PGDLRFDPEYRMLYTDLTREDLWNMPGATIASGPY"
     gene            complement(1317..2891)
                     /gene="terL"
                     /locus_tag="VG274_06705"
     CDS             complement(1317..2891)
                     /gene="terL"
                     /locus_tag="VG274_06705"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HWE06379.1"
                     /translation="MTMIASGAANERARVSPEEYRLLLQNDFHTFLHRSFCELNPRTP
                     FLDNWHIAVLAAKLESVRLGKTKRLIVNLPPRHLKSHAASVAFPAWWLGHDPAKELLC
                     VSCGLELSDKLSRDCRTLMTSALYEAHFPTRLSPTRAAVEEFATTGHGFRLATSVGGV
                     ATGRGGDAIIVDDPLKPEEAMSDNARARVNEWYDNTLYSRLNNRRTGAIVIIMQRLHE
                     DDLVGHVLQQEEWDVVSFPAIAEADESHLVETWQGSCVFGRKAGEPLHEAREPLDELK
                     RLKRTLGGYNFAGQYQQTPSPLGGGMVKRDWFQYYDAAPERFERIVQSWDTANKATEL
                     ADYSVCTTWGVERKHYYLVKVLRQKLNYPELKRAVRAEAELFRPEIVLIEDRASGTQL
                     IQELINDGLYQVKKIAPEGDKVMRLHAQTAAIENGFVHLPREAPWLETYLAEMMAFPR
                     GRHDDQVDSTAQALAWFAVPRGEQGWLEYARRQLEKAGQDTGNKPPAPKPPRQLVETK
                     TRDPMTGEERTFLRWE"
     gene            complement(2978..3529)
                     /locus_tag="VG274_06710"
     CDS             complement(2978..3529)
                     /locus_tag="VG274_06710"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HWE06380.1"
                     /translation="MSDVSNGGGAVGYKRPPREHCWNKGQSGNLKGRPPGFRNFRAAV
                     TAILHEQVSVSVDGEDREMTRLESVARQLVGKAEAGDPRLMQQLLAEIHKNEAKAERD
                     ASAQPLADADRQVIDAFLARLAREAASQNAQTDVGDTGPALAAHGSEPRSPEPADTGD
                     GRRGEAQERAGRQSPEPPDPDQA"
BASE COUNT          701 a         1339 c         1333 g          726 t
ORIGIN      
        1 cagccaccac accgcccaat gccccgcccg ccacaccttc gagcacaacc cagagcgcaa
       61 cgcccgcgcc gatcggaacg acaaactgcg cgccttccac aacttcagcg ggaggatgcg
      121 cacccggcac cacgccgccg cccaactcga tgagcacgcc gccggagacg gcctatccgc
      181 tccagccgcc cggtccgtaa tcggcagccc gcgccggaac tcgcgcattc gacgccgcgt
      241 ttgaatgaga gggggaaagg agacagtcat gcgtagcagg agcatcattc tctccggtgc
      301 ggcgacgctc gttttggcga cgggaggcgc gttggcgcaa tccggttatg gcgacggccg
      361 ctacggcaac gggccggcag aatcgacgcc ggctgaaatg cagcagacgg gccagctcaa
      421 tcagcagtcg gtgaacggaa cgacgcaatc gccagccgtg ctcaacggcg agcaggcgcc
      481 gcagaacggc cccgtcaacg acgaaggcgc cgcgccggat acccagtccc agccgatgac
      541 ccagacgcag tatgcgcaac cgcagtcgca gggcgaatac aacgcgcagc agcagcaata
      601 ccaggaccag atgcagcaat accgcgacca gcggcagcgc taccgcgacg accgcgaccg
      661 ctatgcgcgg aatctgcgcg actgggacct ggcgcaatat gcgtggtact acccgcggcc
      721 ttacgtttat cgctacggcg acgaatacgg attgcagccg ctctatctga tcgcggagcc
      781 gagccaccag cttgcgcagc tgccggtcga aggaccgggc ggacggtggg tcgggcgcgt
      841 gcgcaatgtc gacatcgcac ccgacgggcg gcccgcgcgc atcgagatcg cgctcaaccg
      901 gcgtgtatcg gtgtgggtgc ggccgggcga tctgcgcttc gatccggagt atcgcatgct
      961 ctacacggat ctgacccgcg aggatctgtg gaacatgccc ggcgccacca tcgccagcgg
     1021 accgtactga tcaccgggcg tcgcctgaca cggagtacgc cgcgccggga aacgtcccgg
     1081 cgcggcggga tcaacgacaa taaatggttg gcacagccga cacgtccgtt ggcgctcgct
     1141 tttcctcccc cgcgatgggg caggtaaagt catgaccgat tgcagaaccg ccgcagaatg
     1201 acagggcagt agccggcggc tattcagggg ggtcaacgat ccgctaccgc cccgtcgtcg
     1261 cgatactgac gttaaccgcg tttggtggcc atcgggcggc gccctacagc cgcgcttcac
     1321 tcccagcgca ggaaggtgcg ttcttcgccg gtcattgggt cgcgagtctt tgtttcgacg
     1381 agctggcgcg gtggtttggg cgcgggcggt ttgttgcctg tatcctgtcc cgctttctca
     1441 agctgccgcc gcgcatattc gagccagcct tgttcgccgc ggggaacggc gaaccaggca
     1501 agcgcctggg cggtggaatc gacctggtcg tcgtgccggc cgcgcggaaa cgccatcatc
     1561 tcggcgagat aggtctcgag ccacggcgct tcgcgcggca gatgcacgaa gccgttctcg
     1621 atcgccgcgg tctgcgcgtg caggcgcatg accttgtcgc cctcgggcgc gatcttcttc
     1681 acctgataga gaccgtcgtt gatcagttcc tggatgagct gggtgcccga cgcgcgatcc
     1741 tcgatcagca cgatttcggg cctgaacaat tcggcttcgg cgcgcaccgc gcgtttcagt
     1801 tcgggataat tgagcttctg ccgcagcacc ttgacgagat agtagtgctt cctctccacg
     1861 ccccaggtgg tgcagacgct gtaatcggcg agctcggtcg ccttgttggc ggtatcccag
     1921 ctttgcacga tgcgctcgaa acgctcgggc gcggcgtcgt agtactgaaa ccagtcgcgc
     1981 ttcaccatcc cgccgcccaa cggcgagggc gtctgctggt actgcccggc aaaattgtag
     2041 ccgccgagcg tgcgcttcag ccgtttgagt tcgtcaagcg gttcgcgcgc ttcgtggagc
     2101 ggctcgcccg ccttgcgtcc gaagacgcag ctgccctgcc acgtctcgac gagatggctc
     2161 tcatccgctt cggcgatcgc cggaaaggaa acgacgtccc actcctcctg ctgcagaaca
     2221 tggccgacga gatcgtcctc gtgcaggcgc tgcatgatga tgacgatggc gcctgtgcgc
     2281 cggttgttga ggcgggagta gagcgtgttg tcgtaccact cgttgacccg cgcccgcgcg
     2341 ttgtcgctca tcgcttcctc gggcttcagc ggatcgtcga cgatgatggc gtcgccgccg
     2401 cggcccgtcg ccacgccgcc cacgctggtg gcgagccgaa agccgtgccc ggtcgtcgcg
     2461 aattcctcca ccgcggcgcg cgtgggcgac aatcgcgtcg ggaaatgtgc ctcgtagagc
     2521 gcgctcgtca tcagcgtgcg gcaatcgcgg gacagcttgt cggaaagctc cagtccgcag
     2581 ctcacgcaaa gaagctcctt cgccggatcg tgaccgagcc accaggcggg aaaggcgacg
     2641 gaggcggcgt gggatttcag atgccggggc ggcagattga cgatcagccg cttcgtcttg
     2701 ccgagccgca cgctttcgag tttcgccgcc agcaccgcga tgtgccagtt gtcgagaaac
     2761 ggcgtgcgcg gattgagttc gcagaagctg cgatgcagga aagtatggaa atcattctgc
     2821 agcaggagcc ggtattcctc cggcgaaacg cgcgcgcgct cattcgctgc cccggaagcg
     2881 atcatggtca tggggaaatt ccttcggggg cggaatcagg gatgctgtgt cgcctggcgg
     2941 tgaatctatt ctctaacgat tcctttatcc gccgttccta agcttggtcc gggtcagggg
     3001 gctcggggga ttggcggccg gcgcgttctt gggcttcccc gcgccggccg tcacctgtat
     3061 ccgccggttc gggagagcga ggctcgctgc catgagcggc aagcgccgga ccggtgtccc
     3121 ccacatcggt ttgcgcattc tgggatgcgg cttcgcgcgc gaggcgcgcc aggaacgcgt
     3181 cgatcacctg ccggtcggca tcggcgagcg gctgggctga ggcgtcgcgc tcggccttcg
     3241 cttcgttctt gtgaatctcc gcgagcagct gctgcatcag gcggggatcg ccggcctccg
     3301 ccttgccgac aagctggcgg gcgaccgatt cgagccgcgt catctctctg tcttcgccgt
     3361 cgacactgac gctgacctgt tcgtgaagaa tggcggtcac ggccgcgcgg aaattgcgaa
     3421 accccggcgg gcggcctttg agattgcccg actggccctt gttccagcaa tgctccctgg
     3481 gcgggcgctt gtagccgacg gccccgccgc cgttcgatac gtcgctcatc atgtccctat
     3541 tttgtgattg gccggcggcg cccgcgccaa tgaaaaaccc ggccgctcct gcgagcgccg
     3601 ggcgcatcat tccgacatgg ccaaatcagt agcgtgtttg atcaaaacgc gccagcttta
     3661 atttcgggcc taccaaaagg cgaacgaaga cgcggcgcgc gcctcgccgg gcaatcgcgg
     3721 cgcagcgcac gcacgcccgt tcctgctggc aaggattttc cagccatctg gcccgtctcg
     3781 ttcatcagaa gcagccgtcg ggatggcatg cggccggtcc agggcgacat tggtgatggc
     3841 gccgatgacg aatttgcgag tgcgatccaa agtttctggt gattatatgg gccgggagaa
     3901 gatgctggcg gctccgaaac cggcaacggc cagtggcccc gttctaacag tcgattcccc
     3961 aaaatgttgt catggcccca ctcgatgggg ccacccagcc agcgcgcgtc cgcgcgctga
     4021 atacacgtat tgagcggtgg cgcctgggtg gcccgctcgg agtttacact ccgaccgcgc
     4081 tctcgcgcgg atcggggtg
//
LOCUS       DASZMC010000330        10954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5082839,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000330 DASZMC010000000
VERSION     DASZMC010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5082839"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..754
                     /locus_tag="VG274_06715"
     CDS             188..754
                     /locus_tag="VG274_06715"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HWE06381.1"
                     /translation="MPGSWTISVAGRTYGPYSAHQMKAFAAEGRLGRQSLVARMGEAN
                     FRKAADEPELSHIFSPPPPREGAVAQSLRRDSSAFGRNDTQPQSGERTHIVIIADMKS
                     GSIARLEEEIATFGQACAILPQVWLLASDMSVTNVHNHLVQQLGKLDMIFVLDATNDR
                     AAWFNFGPEVDARIRKVWRTESKLRAAS"
     gene            complement(1038..1568)
                     /locus_tag="VG274_06720"
     CDS             complement(1038..1568)
                     /locus_tag="VG274_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWE06382.1"
                     /translation="MAIYTLDDARPEFPAAAEFWVAPNASLIGRVRLLKNASVWFGAV
                     LRGDNEWIEVGENSNIQDNSVVHTDPGLPTKIGANVTVGHNVILHSAIIGDTTLIGMG
                     STVLSGAKIGPNCLIGANSLIGEKKEIPANSLVLGAPARIIREVSPSQIALIRMSAEI
                     YVQNHRRFRAQLRDLE"
     gene            1850..2596
                     /locus_tag="VG274_06725"
     CDS             1850..2596
                     /locus_tag="VG274_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWE06383.1"
                     /translation="MAKRSARRRDRDLQLPDSATIHPLFPPRPAYDPLDPQGRDRKYV
                     KNVRAMSATQQRLMDAVDAHSVVAALGPAGTGKTYLAIAKAVEALEQNRVGRIVLSRP
                     AVEAGESLGFLPGDLQDKLAPYLRPLYDALTERLGTKRLKTLVAEGAIEIAPVAYMRG
                     RTLNDCFVVIDEAQNCTYGQLKMLLTRLGWRSTMVLTGDPDQTDLLSGLSGFGEIANR
                     LDRVAEVAVIRMGESDIVRHPLVASMLTVL"
     gene            complement(2583..2870)
                     /locus_tag="VG274_06730"
     CDS             complement(2583..2870)
                     /locus_tag="VG274_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06384.1"
                     /translation="MVALFAMTAGFTASGISANLYHLLVKEARSSVDRAIFVAVMIVA
                     GPSVLFEKAAKAWREKACSGVAFWLAAALAGYWSLALGLLVIQVALALKAR"
     gene            3111..3860
                     /locus_tag="VG274_06735"
     CDS             3111..3860
                     /locus_tag="VG274_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06385.1"
                     /translation="MHAAEPMSRQGSEFDGQVKERSGWLLPIAVFVVTAVLSALFLLY
                     YLAPNPGSFIEEHPQPTSRTDAVKLWIGGIPFTIPANYLLYPGARKGGARREVALYAA
                     LPDFRGYSDADGPVFSGNNAGSPIVYMLIREEAFNLPEATRLQRIYLNDVIDVRGRKA
                     PFGLVQYEFRNDSGYRGEDLFVGQIGRGVVVMHCVRLSQNVPSPSCLRDTMLARGVAL
                     SYRFKRSQLASWRDIANGADRLVASFRVPGK"
     gene            complement(4111..4344)
                     /locus_tag="VG274_06740"
     CDS             complement(4111..4344)
                     /locus_tag="VG274_06740"
                     /inference="COORDINATES: protein motif:HMM:NF022768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HWE06386.1"
                     /translation="MTRTEVWRLEKYLRNLFRLDTITLIERPKQPDSVEMHVAGEFIG
                     VIFRDDEDGEVSYALQMAILEMDLPEPPPSRAG"
     gene            4460..5734
                     /locus_tag="VG274_06745"
     CDS             4460..5734
                     /locus_tag="VG274_06745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWE06387.1"
                     /translation="MPEQQPQGYYAASVSPEPPRPALNGRRTCDICVVGGGYTGLSAA
                     LHAAVTGARVVLLEAQCVGFGASGRNGGQIHTGLRKVQSELEAWLGPMHARDLWSLSE
                     EAKALLRALVARHEIACELKTGLIIAAHDTGAARALAKETEHLERSYGYRAMRMLDAA
                     ETAKQTGSRLYRSARFDEGGGHLHPLLYARGLARASEAAGAAIFEKSRVEAIDTQGRA
                     ELRCANGSIAADQVILATDAYSGAVAPELDRFIGHVESFVTATAPLPPSLASEILPGD
                     AAVADTRHVLDYYRKSADERLLFAGREAYFGLPADIAALVRPRMLRVFPQLRDVATEY
                     AWSGTVGITVTRMPHFGRLSDRVLYAHGYSGQGVALATLGGRLLAEAAMGHNERFEVF
                     ARVPTRPFPGDRWLRRPLIAAALLAYKLADAI"
     gene            complement(5748..6293)
                     /locus_tag="VG274_06750"
     CDS             complement(5748..6293)
                     /locus_tag="VG274_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE06388.1"
                     /translation="MSWLEYDSHPDRVRRGKAMKAWPLLACSFVALIFVAVPARAQMS
                     VGAQELLQKHLVAVDNGRQMNIVCIGHGVPTVVFEDGPGSHMLHWQKVQEPISAMTRA
                     CFHDRAGYGFSDPSKKPMTADNVTNDLHAFLHNAGIAGPLVLVGHSIGGLYATLRVAM
                     LTQMHSPPCRAFCSLRSVSRL"
     gene            complement(6311..7708)
                     /gene="lpdA"
                     /locus_tag="VG274_06755"
     CDS             complement(6311..7708)
                     /gene="lpdA"
                     /locus_tag="VG274_06755"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWE06389.1"
                     /translation="MADQLDVLVIGSGPAGYVCAIRAAQLGLRTAIVERDRLGGICLN
                     WGCIPTKALLRSSEIWHLMHRLKEFGFSADNLQFDLARIVERSRKVAAQLSNGVAFLM
                     KKNKIEVVPGEARLSGGGKVAVVKDGATRELSAKHIVLATGARARALPGLEPDGKLIW
                     TYREAMVPPSFPKSLLVVGSGAIGIEFASFFRTLGSEVTVVEVLDRILPVEDEEISQF
                     AAKAFSKQGMKIRTSTTVENLEKGADAVTATLKTKDGKTETLHAERAVLAVGIVGNVE
                     NIGIEEAGVKVEKTHVVVDEWGGTGVNGLYAIGDLVGPPWLAHKGMHEGVIVAERIAG
                     VAGVHPLDTSKVPACTYCWPQIASVGMTEAAAKASGCKIKVGRFPYIGNGKAIALGDP
                     EGLVKTVFDADTGELLGAHMIGAEVTELIQGYAIARTLEATEAELTTAVFPHPTLSET
                     MHESVLDADRRALHI"
     gene            complement(7716..8906)
                     /locus_tag="VG274_06760"
     CDS             complement(7716..8906)
                     /locus_tag="VG274_06760"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HWE06390.1"
                     /translation="MPDDLGAIAELGGAAAVEIAASALAERGAKTTKCRNCGAPVIGA
                     YCAVCGQERDAHRRSLFKLLHDLFEEIVSFDSRVLRTVIALLFKPGELPLAFHEGRTR
                     RYMPAVRLYLFVSLLFFLTLSVAGIALVQIQIKEVPKAYVVRMLPNGDAVIIAGGKAS
                     RPILAAEIRKALHRQGFNVGGTDAMKEGKASNSFTAQSISPGVHSGLEPDVSFFRPIA
                     AHPPRITPAGWAQIEQMKTDVLRDVGNDKNGWMARNALATIEKLTRDPAALNGPLTIW
                     IPRVFFLLLPLFACLLALFYVRQRKQFYFVDHLVFSLSVHSFVFAILIVAIGAAQLLP
                     GGVVAWIILLSIGFYTFIALKRFYRQGWTATGLKFAAITFIYVVFVQAPALLFVLATG
                     FIEG"
     gene            complement(9119..10492)
                     /locus_tag="VG274_06765"
     CDS             complement(9119..10492)
                     /locus_tag="VG274_06765"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HWE06391.1"
                     /translation="MPTQILMPALSPTMEEGKLARWFVKEGDEVRSGDLLAEIETDKA
                     TMEFEAVDEGRVGKILVPEGTEGVKVNQPIALLLGEGEESSAAPPAPATSSAAKRKNG
                     EAPRAEMRASEPQHHDIASAMSSIREALASEAPAESAGAESERVFASPLARRMAQQAQ
                     IDIGAIKGSGPRGRIVKADVEAAEGKTAAPQEAGAVQRKAKPAQPAPAPAVPGLAPLA
                     DARLFYKPEDYEEVPHDAMRKSIARRLLTAKTLIPHYYLTVDCRIDELLALRARLNAA
                     SPKGEGGYKLSVNDFVVKASALALLRVPEVNASWTDTAILRHRHADVGVAVALDFGLI
                     TPIVFRADEKGLGAISNEVKSLAQRARAKKLKPQEYEGGGFAISNLGMFRIRDFTAII
                     NPPHAAIIAVGAGEERAIVRDGRIEAANMMTVTMSRDHRVIDGATGARFLGFFRQFVE
                     DPAAMLL"
     gene            complement(10494..10883)
                     /locus_tag="VG274_06770"
     CDS             complement(10494..10883)
                     /locus_tag="VG274_06770"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWE06392.1"
                     /translation="MTWRKGGCHCGAVRFEVLAADEIEVQACNCSICHMTGYLHFIVS
                     KSRFRLLEGRDQLTTYAFNTETAKHMFCSICGIKSFYVPRSHPEGYSVNFRCMNAEEF
                     HSVLTTEFDGRNWEQHISNLESLGNGS"
BASE COUNT         2088 a         3461 c         3322 g         2083 t
ORIGIN      
        1 ggccggtcga gtgtaacgat ggtaagcggc ccttcccgct ccacccggca atattgatgc
       61 actgccagct ccccgttccc ctctcgggtc ctcaaaaaca agcttggcgc ggccgcctga
      121 cgcggcgcaa gcctcgatta accatttgaa actaatttgg cgcggcaaat catggaagcg
      181 tccctcaatg ccgggctcgt ggacgatcag cgtcgccgga cggacctacg ggccttacag
      241 cgcccaccag atgaaggcgt tcgccgccga gggccggctt gggcgccagt cgctcgttgc
      301 tcgcatggga gaggcgaatt tccgcaaggc tgccgacgag ccggaactgt cgcacatttt
      361 ttcaccgccc ccacctcgcg agggagccgt tgcgcagagc ctgcggcgcg acagcagtgc
      421 gttcgggcgc aacgacaccc agccgcaaag cggcgagcgc acgcacatcg tgataatcgc
      481 cgacatgaaa tcgggatcca ttgcaaggct cgaggaagag atcgccacgt ttggccaggc
      541 ttgcgcgatc ctcccccagg tctggctgct ggcgagcgac atgtcggtga cgaacgtgca
      601 caatcacctc gtgcagcagc tcggcaagct cgacatgatc ttcgtccttg acgcgaccaa
      661 cgacagggcc gcctggttca atttcggtcc ggaggtcgac gcccgaatcc gcaaggtttg
      721 gcgaacggaa tcaaaattac gcgccgcgag ctagcgcatc gactcagaca ccctggtgac
      781 tgggttgatg cctctcacga agccgacaag gcacgaagaa gaaaaaagac tttagcgcag
      841 aggaagcgca ggggacgttg agaaagtttg gcgcgccgaa ggcgcgcggt aatttctcct
      901 gcgtccttcc gcctacgcca aggcttcggc ggaccaatag agtcattaga cccgccgtag
      961 ctttagcgaa ggcgggccgc gcgggccccg cgtcctctgc gctgaatcct ttattgagac
     1021 aaaactcggc ggacggttta ctcaaggtct ctgagttgcg cgcggaagcg gcggtggttt
     1081 tgcacatata tttccgccga catccttatc agtgcgatct gtgacgggct gacctcgcgt
     1141 attattcggg cgggggcgcc cagcacgagc gagttggcgg gaatttcctt cttttcgccg
     1201 atcagcgaat tggcgccgat gaggcaattg ggtccgatct tcgcgccgct caacaccgtc
     1261 gagcccattc caatcagcgt ggtgtcgccg attatcgccg aatggagaat gacgttgtgg
     1321 cctacggtca cgtttgcacc gatctttgtc ggcaggccgg gatctgtgtg cacgacggaa
     1381 ttgtcctgaa tgttcgaatt ctctcccacc tcgatccact cgttgtcgcc gcggagcacg
     1441 gcgccaaacc acacgcttgc atttttcagg agcctgacgc gcccgatgag ggaggcgttg
     1501 ggcgccaccc agaactctgc cgcagccgga aactccggcc ttgcgtcatc gagcgtgtag
     1561 attgccatgt tcggtttccg catcggacaa gaatagagtg cgcgagtaaa tcagcgcctt
     1621 caagcgccgt tccacggcgc caaaaaaaac ggacggaaca cgcgacgcag aaccgaaaac
     1681 gcgctaacct cgattcgcgc actgagtcgc aatcgggggc accatctgtg cgggaccctt
     1741 cgtcgccgcg aattacttcc gcgagcgttt ccgggcgccg gctgtcatgc tggcgcaatc
     1801 tttccactat caactctatc catgatgcgt cattgaccaa ggagtctcca tggccaagcg
     1861 ttccgcgcgt cgaagagacc gcgatcttca gcttcccgac tccgcaacca tccaccctct
     1921 tttccccccg aggccggcat acgatccgct cgatccgcag ggacgcgatc gaaaatatgt
     1981 aaagaacgtg cgggccatga gcgcgacgca gcagcggctc atggatgcgg tcgacgccca
     2041 cagcgttgtc gcggcacttg gtccggcggg aacgggcaag acctatctcg ccatcgcgaa
     2101 ggccgtggaa gcgctcgagc agaaccgcgt cggccgaatt gtcctgtcgc ggccggccgt
     2161 cgaagcaggg gagagtcttg gatttctgcc gggcgatctg caggacaagc tcgcgcccta
     2221 tttgcgcccg ctgtacgacg cgttgacgga gcggctgggc accaagcgac tcaagacgct
     2281 cgtggcggaa ggagcgatcg agatcgcgcc tgtcgcctat atgcgcggcc gcactctcaa
     2341 tgactgtttt gtcgtcattg acgaggcaca aaactgcact tatgggcaac tcaaaatgct
     2401 tctgacgcgg ctgggctggc gttccaccat ggtgctgacc ggcgatcccg atcagacgga
     2461 cctgctcagc ggactgtcag gcttcggcga gatcgcaaat cgcctggaca gggtggcgga
     2521 agtggcggtc atccgcatgg gcgagagcga catcgtgcgg cacccgctgg tggcgagcat
     2581 gctcaccgtg ctttgagggc gagcgcgacc tgaatgacaa gcaagccgag cgcgagactc
     2641 cagtagccgg cgagcgccgc agcgagccag aaggcgacac cggagcaggc tttctcgcgc
     2701 caggctttcg cggccttttc gaacaggacg gaggggccgg cgacaatcat gaccgccacg
     2761 aaaattgcgc gatcgaccga ggaacgcgcc tccttgacga gcaggtgata gaggttggcg
     2821 ctgatcccgg aggcggtgaa gcctgccgtc atcgcgaaga gggctaccat cgcgtcgtgc
     2881 ataactgacc cacccggcca cctttggcgg aggggatgca gcgaccgtgc ctgcaaaatg
     2941 gcggctaaaa ccgccctgcc gtcccatagt gggacgtccg gcgcaaatgg acggccgatg
     3001 cccgatgacc ggacggcggt tcgcttattt ccgcccaggc tgacctgcgt gacgatttgt
     3061 tcggctggcg ccggcaacga atcggggcat gttcgccgtt ggggcagaac gtgcacgcag
     3121 ccgaaccgat gagccgccaa ggcagcgaat tcgacgggca ggtaaaggaa cgatccggct
     3181 ggctgttgcc gatcgccgtc ttcgtcgtga ccgccgtcct ctccgcgttg ttcctgctct
     3241 attatctggc gccgaatccg ggctcgttca tcgaagaaca tcctcagccc acgtcgcgca
     3301 cggacgccgt gaagctgtgg atcggtggaa tccccttcac aatccccgcc aactatctgc
     3361 tctatcccgg cgcgcgaaag ggcggcgcgc gcagggaagt cgcgctctac gcggcgctcc
     3421 ccgactttcg gggctactcc gatgcggatg gtcccgtttt ctcgggaaac aacgcggggt
     3481 cccccattgt gtacatgctg atccgggagg aggcgttcaa tcttccggaa gcgacgcggc
     3541 tgcagagaat atatctgaac gatgtcatcg atgtgcgcgg ccgcaaagcg ccattcggac
     3601 tggtacaata cgaattccgc aacgattcag gctatcgcgg cgaagatctg ttcgtgggac
     3661 agatcgggcg cggcgtcgtc gtaatgcact gcgtccgcct gagccaaaac gtgccaagcc
     3721 ccagctgcct gcgcgacacg atgctcgccc gaggcgtggc gctcagctat cgattcaagc
     3781 ggtcgcaact cgccagctgg cgcgacatcg ccaacggcgc cgaccgcctc gttgcctcgt
     3841 tccgggtgcc gggaaagtaa ctaccggtag tgttcgcgat cggcgggctg cggtggcctg
     3901 attcggccac gcctcattcc cacaatttgt catggcccgc caccccgatc cgcgcgagag
     3961 cgcggtcgga gtgtaaactc cgagcgggcc gcccagacga aaccgctccg agtgtttatt
     4021 cagcgcgcgg acgcgcgctg gctgggtggc ccgtaaagcg ggccatgaca atttttttgt
     4081 ggttcagaac cagaccactt gcgcatgccc ctatcccgcc cgcgagggcg gcggttcggg
     4141 caggtccatc tccagtatcg ccatctgcag ggcgtaggag acttctccgt cctcgtcgtc
     4201 cctgaatatc actccgatga attcgccggc gacatgcatc tcgacggaat cgggctgctt
     4261 cggccgttcg atcagggtga tcgtgtcgag ccgaaacaga ttgcgcaggt atttttcgag
     4321 gcgccagact tccgtgcggg tcaagtcgca tgctcccaac tgaatgcgcc tgaactcgca
     4381 tgcgaacggg cgactggtca agacgacggt atcggagatt gcccttgacc gcacgccgcg
     4441 cctgtagtcg caatcggtca tgcccgagca gcaaccgcaa ggctactacg ccgcgagcgt
     4501 ttcgcccgaa ccgcccaggc ctgcgctgaa cggccggcgg acgtgcgaca tctgcgtggt
     4561 tggaggcggc tataccggcc tttccgccgc tctccacgca gccgtgaccg gtgcgcgcgt
     4621 cgttctgctc gaggcgcaat gcgtcggctt cggtgcttcc ggaaggaacg gcggacaaat
     4681 ccataccggc ctgcgcaaag tgcagagcga gcttgaagcc tggctcggcc caatgcatgc
     4741 acgcgatctc tggtcgctga gcgaagaggc aaaggcgctt ctgcgtgcgc tcgtcgcgcg
     4801 ccatgaaatt gcgtgcgaat tgaaaacggg cctcatcatc gcggcgcacg acaccggcgc
     4861 cgcgcgcgcg cttgcaaagg aaacggagca tctggagcgt agctatggat atcgcgcgat
     4921 gcggatgctg gacgccgcgg agaccgcgaa gcagacggga tcgcgtctct atcgcagcgc
     4981 ccgcttcgac gaaggcggcg gccatttgca tcctcttctc tacgctcgcg gcctggcgcg
     5041 cgcgtcggag gccgcaggcg ccgcgatctt cgaaaagtcg cgcgtcgaag caatcgacac
     5101 gcagggacgc gcggaattgc gctgtgcaaa cggcagcatc gcggccgatc aggtcatcct
     5161 ggcgaccgat gcttattcgg gcgccgtcgc gcctgaactc gaccgcttca tcggccacgt
     5221 cgagagtttc gtcaccgcca cagccccgct gcctccctcg ctagcgagcg agatattgcc
     5281 cggcgatgcc gcggtggccg acacgcgcca tgtgctcgac tactaccgca agagtgcgga
     5341 cgaacggctg ctgtttgcgg gccgggaagc gtatttcgga ttgccggccg acatcgcagc
     5401 cctggtcagg ccgcgcatgc tgagagtctt cccgcagctt cgcgatgtcg cgaccgaata
     5461 cgcctggagc ggtactgtcg gcatcaccgt tacgcgcatg ccgcatttcg gaagattgtc
     5521 cgatcgcgtc ctgtacgcgc acggctattc cggtcagggg gtcgcactgg cgacgctcgg
     5581 cggcaggctg ctcgccgagg cggcgatggg acacaacgaa cgcttcgaag tcttcgcgcg
     5641 cgtccccacc cgccccttcc ccggcgaccg ttggctgcgc cgcccactga ttgcggccgc
     5701 actgctggcg tataaattgg ccgacgcaat ttaagcgcga gcttccatca gagacgtgag
     5761 acggatcgaa gcgaacagaa tgctcgacaa ggcggggaat gcatttgtgt aagcatcgcc
     5821 actctgagcg tagcgtagag cccgccgatc gaatgcccga caagcacgag cggtccggcg
     5881 atcccggcgt tgtggaggaa ggcatggagg tcgtttgtta cgttgtccgc cgtcatgggt
     5941 tttttggatg gatcgctaaa tccgtatccg gcccggtcgt ggaagcaagc cctggtcatg
     6001 gcagagatcg gttcttggac tttctgccaa tgcagcatgt ggctgcccgg gccgtcctcg
     6061 aatacgacgg tcgggacgcc gtggccgata caaacgatgt tcatctggcg gccattgtcg
     6121 acggccacga gatgcttttg taacagttct tgagcgccga cagacatttg tgcacgggcg
     6181 ggaacagcca cgaagatcaa tgccacgaag gaacatgcaa gcaacggcca cgccttcatc
     6241 gcctttcccc gtctgacacg atcaggatga gaatcatatt cgagccaaga caccgggcca
     6301 cggcatgctc tcaaatatgc agagcgcgcc tgtcggcgtc gagcacggat tcgtgcatcg
     6361 tttcggacag tgttgggtgc ggaaaaacgg cggtcgtaag ctcggcttcg gtggcttcga
     6421 gggtgcgcgc gatcgcgtag ccctggatca attcggtaac ctccgcgccg atcatgtgcg
     6481 cgcccagcaa ttctcccgta tcggcatcaa acaccgtctt caccagaccc tcgggatcgc
     6541 caagcgcaat cgccttgccg ttgccgatgt acggaaaacg gccgaccttg atcttgcatc
     6601 ctgaggcctt tgcggcggct tccgtcatcc ccacgctggc gatctggggc cagcaatagg
     6661 tgcaggcggg aaccttcgac gtgtcgagcg gatgcacgcc cgcaacgccc gcgatgcgtt
     6721 cggcgacgat cacgccctca tgcatcccct tgtgcgcgag ccagggcggt ccgacgagat
     6781 cgcctatcgc atagagtccg ttcacgcccg tgccgcccca ctcatcgacg accacgtgcg
     6841 ttttctcgac cttcacgccg gcctcttcga tcccgatatt ctcgacgttg ccgacaatgc
     6901 cgacggcgag gacggcgcgt tcggcgtgga gcgtttccgt cttgccgtcc tttgtcttga
     6961 gagtggcggt gacggcgtcg gctcccttct ccagattctc tactgtcgtc gaagtccgga
     7021 tcttcatgcc ctgcttgctg aaggctttcg cggcgaattg cgaaatctcc tcgtcttcca
     7081 ccggcaggat ccggtcgagc acttccacca ccgtcacctc gctgccaagc gtccggaaga
     7141 agcttgcgaa ctcgatgccg atggcgcccg agccgacgac gagcaaagat ttgggaaagg
     7201 aaggcggcac catcgcctcg cgataggtcc agatcagctt gccatccggc tcgaggccgg
     7261 gcagcgcgcg cgcgcgcgct ccggtggcaa ggacgatgtg ttttgccgaa agttcgcgcg
     7321 tcgcgccgtc tttcacgacc gcaacctttc ccccgccgga aagcctggct tcgcctggga
     7381 cgacttcgat cttgttcttc ttcatcagga aagcgacgcc gttggacagc tgcgcggcaa
     7441 ccttgcgcga gcgctcgacg atcctggcaa gatcgaactg cagattgtcg gcagagaatc
     7501 cgaattcctt cagccggtgc atcagatgcc agatttccga cgagcgcaga agcgccttgg
     7561 tcggaatgca accccagttg aggcatatcc cgccgagcct gtcgcgttcg acgatcgcgg
     7621 ttctgagccc cagttgcgcc gcgcggatgg cgcagacata gcccgcgggc ccgctgccga
     7681 tcacgagtac gtccaattgg tctgccatgg tttcttcagc cttcgataaa gccggtcgct
     7741 aacacgaaca gcagagccgg cgcctgcacg aaaacgacat agatgaatgt aatcgccgcg
     7801 aatttgagtc cggtcgctgt ccatccctgc cggtagaagc gcttgagcgc tatgaaagtg
     7861 tagaatccaa tcgacagaag aatgatccag gcgaccacgc ccccgggcaa aagctgcgcg
     7921 gctccgattg ccacgatgag gatcgcaaat acgaagctgt gaacggagag cgaaaagacg
     7981 agatgatcca cgaagtagaa ctgctttcgc tgcctcacat agaacagcgc gagaagacat
     8041 gcaaacagcg gcagcaaaag aaagaatacg cggggaatcc agatggtcag cggcccgttg
     8101 agcgccgcgg gatcgcgggt cagtttctcg atcgtcgcca gggcgttccg tgccatccat
     8161 ccgttcttgt cattgccgac atctctcagg acatctgtct tcatttgttc gatttgcgcc
     8221 cagccagctg gcgtaattcg cggcggatgc gccgcgatgg gcctgaaaaa actgacatcc
     8281 ggttcaaggc cggaatgaac accgggcgag atgctctgcg ccgtgaaact gtttgacgcc
     8341 tttccctcct tcatcgcgtc ggtaccgccc acattgaagc cttgcctgtg caatgccttg
     8401 cggatttccg cagccagtat gggccgactt gcctttcctc cggcaatgat gacggcatcg
     8461 ccgttcggca acatcctgac gacgtaggcc ttcggcactt ccttgatctg gatttgcacc
     8521 agcgcgatgc cggccaccga cagtgtgaga aagaacagca gcgatacaaa cagatagagg
     8581 cgcaccgcag gcatatagcg gcgcgtccgg ccctcatgga acgcgagcgg cagctcgccc
     8641 ggcttgaaga gcagcgcgat cacggtgcgc aggacgcggc tgtcgaaact gacgatctcc
     8701 tcgaagagat cgtggagaag cttgaacagc gagcgcctgt gcgcgtcgcg ctcctggccg
     8761 cagaccgcgc aataggctcc gatcacgggc gcgccgcaat tgcggcattt cgtggtcttc
     8821 gcgccgcgct cggcaagcgc cgaagccgca atctcgacgg cagccgcgcc tcccagctcc
     8881 gcaatcgccc ccaaatcatc cggcatcggt tacgccccgg tatcaacttt cgatcgtgga
     8941 cgagtcgtgc accggcacat ttttaacgca gggtacgcgg aggatgcgcc gaggacgcag
     9001 aggaatttct tgcgcacttc gcgcgctatc attttcctgc gtccactgcg catcctccgc
     9061 gccctctgcg ttaaatctac ctccgtgtca ctcttctttg actcccggta ccaggcactt
     9121 atagcagcat tgcggcgggg tcttcgacga actggcggaa aaagccgaga aaccgcgcgc
     9181 cggttgcgcc gtcgatcacg cggtggtcgc gggacatcgt caccgtcatc atgttggccg
     9241 cctcgatcct gccgtcgcga acgatcgcgc gctcttcgcc tgctccgacc gcgatgatgg
     9301 cggcgtgggg cggattgatg atggcggtaa agtctctgat cctgaacatg cccagattgg
     9361 agatcgcaaa cccgccgcct tcgtattcct gcggcttgag tttctttgcg cgcgcgcgct
     9421 gcgccagcga cttcacctcg ttcgaaatcg caccaagccc cttttcatcg gcgcgaaaga
     9481 cgatcggcgt gatcaggccg aaatccagcg caaccgcgac cccgacgtcc gcgtggcgat
     9541 gcctgaggat cgcggtgtcg gtccagctcg cattgacctc gggcacgcgc aacaaggcaa
     9601 gcgccgaagc cttgaccaca aaatcgttga ccgaaagctt gtagccgcct tcgcccttcg
     9661 gggaggccgc attgaggcgc gcgcgcaggg cgagcaattc gtcgatgcgg caatcgaccg
     9721 tgaggtaata atgcgggatg agagtcttgg ccgtcagcaa tcgacgcgcg atggatttgc
     9781 gcatcgcgtc atgcggcact tcctcgtaat cttccggttt gtaaaacagc ctcgcatcgg
     9841 cgagcggcgc caacccgggc acagcgggcg ccggcgcggg ttgtgccggc ttcgccttcc
     9901 tttgcaccgc gccggcttcc tgcggcgccg ccgttttccc ctcggccgct tcgacatccg
     9961 ccttgacgat acgtccgcgc ggtccggaac ccttgattgc gccgatatcg atttgcgcct
    10021 gttgcgccat gcggcgcgca agcggtgacg cgaagacgcg ctcgctctcg gcacccgcgc
    10081 tctcggccgg cgcctcggac gccagcgcct cgcggatgga ggacatcgcc gaagcgatgt
    10141 cgtggtgttg cggttcggac gcgcgcatct cggcgcgggg cgcttcgcca tttttccgct
    10201 tggctgcaga tgatgtcgcc ggcgccggcg gagcagcgga tgattcctcg ccctcgccca
    10261 gaagaagcgc aatcggctga ttgaccttca cgccttccgt gccttcgggc acgagaattt
    10321 tgccaacccg cccttcatcg acggcctcga actccatcgt cgccttgtcg gtctcgatct
    10381 cggcgaggag atcgccggaa cgcacctcat cgccttcctt gacgaaccaa cgggcaagtt
    10441 tgccctcttc catggtcggc gaaagtgcag gcatcagaat ctgcgtcggc atttcaactc
    10501 ccgtttccga gggattcgag attggaaata tgctgctccc aattgcgccc gtcgaattcc
    10561 gtcgtcagca ccgagtgaaa ctcttccgcg ttcatgcagc gaaaattcac gctgtatcct
    10621 tccggatgcg agcgcggcac gtagaacgat ttgatcccgc atatcgaaca gaacatgtgc
    10681 ttcgcggttt cggtgttgaa cgcgtatgtc gtgagttgat ctctgccctc aagcaggcgg
    10741 aatcgcgatt tggatacgat gaagtgaaga taacccgtca tatggcagat cgaacaattg
    10801 caggcctgca cttcgatttc gtcggccgcg agaacctcga accgcaccgc accgcagtgg
    10861 cagccgcctt tcctccaggt caatcccttc ccgaccatcg cggcatcatt cgcggtagag
    10921 aaccgatttc gcagcggcga tcacctcgtc gatc
//
LOCUS       DASZMC010000331         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4811486,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000331 DASZMC010000000
VERSION     DASZMC010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4811486"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..841
                     /locus_tag="VG274_06775"
     CDS             113..841
                     /locus_tag="VG274_06775"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HWE06393.1"
                     /translation="MAPLLRNLAAALLTLTAAGCATAPNGPGADQNDPYETQNRKMFE
                     LNQSLDRNFMLPVAKGYVAVVPDPARDGIHNFLVNLNLPVTFANDVLQGEVSRAGETV
                     GRFTVNSTVGIGGLIDVAEKIGIPEHKSDFGETLAVYGVGEGPYLVLPLLGPSNPRDA
                     TGYAVDVALNPWTWISWRSSIFFKLGEDSLQIVDERSRNIETLDELERSSVDLYATLR
                     SLYRQHRQAEINHGKANIENLPNF"
     gene            882..>2926
                     /locus_tag="VG274_06780"
     CDS             882..>2926
                     /locus_tag="VG274_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWE06394.1"
                     /translation="MPAPIVERIVGFCARHAGAIIVAALVASILACFYSATHFAMNTD
                     AESLISPQTTWRKRVAAFDSEFPQLGNSIAIVIDGATPELAERGAAELSAALEGRGDL
                     FHDVRRPDGGPFFAHEGLLFFPLAQVRSTTQQLIAAQPFLGALAIDPSLRGIMDSLST
                     ALQGVQRGQASLDSLARPIGAFSSTLDAVAARKPAFLAWGSLITGAPPNSLEMRRFVE
                     VQPKLDYRALMPGKHATNSIRALARRLNLTPDAGIRVRLTGPVPMADEEFSTIKDRAR
                     LMVALMTSGVLVMLWLAVRSVRIILAILLTILVGLAATTAIGLLVVGSFNIISVAFIA
                     LFVGLGVDFGIQFCVRYRAERHSEDDLDLALRNAGSGVGNALTLAAGATAAGFYSFLP
                     TSYSGVAELGLIAGTGMLVTFGLSITLLPALLKLLRPGGEPDAIGYRVLAPLDRTLGE
                     GRVIVLAVAVLLGIVCIAALPRLTFDFNPLDLRSPKGEAVGTALDLMRDPATTPNTID
                     VVVPSNEAARRVSAALSALPAVSNVVSINSFIPDDQKPKLALIRDADSLLDATLNPFL
                     TKPPPSDRDVVASLRAAARALDSAIAGAHGQAAQHAKHLAGVLDMIARGAPDLRRHAQ
                     ASLVPGLQTMLDQLRAALTATPVSRASLPKSFARDWVTPDGKYRLQVFPRGNANNNAV
                     "
BASE COUNT          482 a          954 c          894 g          596 t
ORIGIN      
        1 gctctcggtt gctgacccgt tgcatcttgg cgcgggcgtg ccccgcactt atgttaaccc
       61 gttacggaat tgcgcccaga tcgttgtgag atgggaccag agagggattt gcatggctcc
      121 gctccttcgg aacctcgccg ccgcgctttt gactctcacc gcggcgggat gcgcgaccgc
      181 gcccaacggc ccgggcgcag atcagaacga cccttatgaa acgcagaacc gcaagatgtt
      241 cgagctcaac cagtcgctcg acaggaattt catgcttccg gtggccaaag gctatgtcgc
      301 ggtggttccg gatcctgccc gtgatggcat tcataatttt ctggtcaatc tcaatttgcc
      361 ggtgactttc gccaacgatg tgctgcaggg cgaggtgagc agagccggcg agacggttgg
      421 ccgcttcacg gtaaattcga ccgtaggcat cggcggcctt atcgacgtgg cggaaaagat
      481 cggcataccg gagcacaagt cggatttcgg tgaaacgctg gccgtctatg gcgttggcga
      541 gggcccctac cttgtcttgc ctttgcttgg ccccagtaat ccgcgcgatg ccaccggcta
      601 tgccgtagat gtcgcactca acccgtggac ctggatcagc tggcgcagca gcatcttttt
      661 caagctcggc gaagactctc tgcaaattgt cgacgaacgc tcccgcaaca tcgaaacgct
      721 ggacgaactg gaacgcagct cggtcgatct gtacgctacg ctgcgcagcc tctatcggca
      781 gcaccggcag gccgagatca atcacggcaa ggcaaatatc gaaaacctgc cgaatttcta
      841 gtcgccggct tgctccatgg gagccgtagg ggggcggtcg catgccagca ccgatcgtag
      901 agcggatcgt gggattctgc gccagacacg cgggcgcgat cattgttgct gcgcttgtcg
      961 cgtccattct tgcctgcttc tacagcgcca cgcatttcgc catgaacacc gatgcggagt
     1021 cgctgatctc gccgcagacg acgtggcgca agcgtgtcgc ggccttcgat tccgaattcc
     1081 cccagctggg caacagcatc gcgatcgtga tagacggcgc cacgccggaa ttggcggaac
     1141 ggggcgcggc ggaactctcc gctgcgctcg aaggtcgcgg tgaccttttt cacgatgtgc
     1201 gccggcccga tggcgggccg ttcttcgcgc atgaaggatt gcttttcttc ccgctcgcgc
     1261 aggtgagatc caccacgcaa caactgattg ccgcccagcc ttttctggga gcgctggcca
     1321 tcgatccgag cttgcggggc atcatggaca gcctgtcgac cgctcttcag ggcgtgcagc
     1381 gcggccaggc gtcgctcgac tcgctggcgc ggccgatcgg cgctttctcg tccaccctcg
     1441 atgccgtggc cgcgcgcaaa cccgcgttcc tcgcctgggg gtcgctgatc acgggtgcgc
     1501 cgccaaattc gctcgagatg cgccggttcg tcgaagtgca gccgaaactc gactatcgcg
     1561 ccctcatgcc gggcaagcac gcgaccaatt cgatccgcgc actcgcgcgc aggctgaacc
     1621 tcacgcccga tgcgggcata cgcgtccggc tcaccggacc ggtaccgatg gcggatgagg
     1681 aattcagcac catcaaggac cgcgcgagac tgatggtcgc gctgatgacg tcgggcgttc
     1741 tcgtcatgct gtggctggcc gtgcgatccg tccgtatcat cctcgccatc ctgctcacca
     1801 ttcttgtggg tctcgccgca accacggcca tcggccttct ggtcgtcggc tccttcaaca
     1861 tcatctcggt cgcgttcatt gctctctttg tcggcctggg cgtcgatttc ggcatccagt
     1921 tttgcgtccg ctatcgcgcg gaacggcatt cggaagacga tctcgacctg gcattgcgca
     1981 atgcgggcag cggcgtgggc aatgcgctga cccttgccgc cggcgccacc gcggcgggat
     2041 tctattcgtt tctgccgacg agttattccg gcgtcgcgga gcttggactg attgcgggca
     2101 ccggcatgct cgtgaccttc ggcctcagca tcacgctcct gccggcgttg ttgaagctct
     2161 tgcgtcccgg tggcgagccg gatgcgatcg gctatcgtgt gctggcgccg cttgatcgca
     2221 cgcttggcga gggcagggtg atcgtcctgg cggtcgctgt cctgctcggc attgtgtgca
     2281 ttgctgcgct gccgagattg actttcgact tcaatcctct cgatctgcgc agtcccaagg
     2341 gcgaagccgt cggcacggcg ctcgatctga tgcgcgaccc cgcaacgact cccaacacga
     2401 tcgatgtcgt cgttccgtcg aacgaagcag ctcggcgcgt gtcagcggcg ctttccgcgc
     2461 tgccggccgt gtccaacgtg gtgagcatca acagctttat tcccgacgac cagaagccga
     2521 agctcgccct tatccgcgac gccgacagtc tgttggacgc cacgctcaac cccttcctga
     2581 cgaaaccgcc gccgtcggat cgggatgttg tcgcgagttt gcgtgctgct gcgcgcgcgc
     2641 tcgactccgc catagcgggc gcgcacgggc aggcagcgca gcacgcaaag catctcgcgg
     2701 gtgtgctcga catgatcgca cgcggtgcgc cggatctgcg ccgccacgcg caggcttctc
     2761 ttgtgcccgg tctccagacg atgctcgacc agctgcgtgc agcgctcact gccacgccgg
     2821 tctcgcgcgc atcgttgccg aagtccttcg cgcgggactg ggtcaccccc gacgggaaat
     2881 atcgcctcca ggtttttccc aggggcaatg ccaacaacaa tgccgt
//
LOCUS       DASZMC010000332         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3695431,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000332 DASZMC010000000
VERSION     DASZMC010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3695431"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VG274_06785"
     CDS             complement(<1..441)
                     /locus_tag="VG274_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06395.1"
                     /translation="MKAWELLVFEFAQALYLRNCPVPEILEKLTEQMAHDDRYRAPDD
                     LSDAEARDRCESIAYLLWERVDSTAADEPDAEQQDWPEPERIEPAFPPAPPMAAEMLP
                     AVVRPFAVDVAECMGAPLDFSAISTIAAASAALGRVVAIRPKAKD"
     gene            complement(669..866)
                     /locus_tag="VG274_06790"
     CDS             complement(669..866)
                     /locus_tag="VG274_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE06396.1"
                     /translation="MSTKHEHELPRLIQLPELARRLDVHRATIWRLIKAGKLPQPIAV
                     TPRIRAFRIEDVAPLFERPGA"
     gene            1063..1362
                     /locus_tag="VG274_06795"
     CDS             1063..1362
                     /locus_tag="VG274_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE06397.1"
                     /translation="MLDALIAAIQTLDPADVPAVMMALSARLLAAKSIAPAESAPDSE
                     DVNLTIAEAAALLRRSGKWVYRHRTSLPFIRKIGPRSYICSKRALDRWLARRQSL"
     gene            1386..1682
                     /locus_tag="VG274_06800"
     CDS             1386..1682
                     /locus_tag="VG274_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWE06398.1"
                     /translation="MTRYSRVVIESFIHKGLKELFETGKSAKVPAHLKQRSDDVLQAL
                     DAAASLKDLNLPGFQLHPLHGSPVRHSIHVSGQWCITFEWQAPRAYRVDLEQYH"
     gene            1695..2024
                     /locus_tag="VG274_06805"
     CDS             1695..2024
                     /locus_tag="VG274_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWE06399.1"
                     /translation="MSDEYVVNRERITRAPAHPGVFFERNILPEFLRQRRTIGEIATL
                     LGVARQTLHRVMAGQTAVTPDMAVRLGKLCGNGPDLWLNMQARYDAWEATRRLGAELK
                     KIPTLTD"
     gene            2133..>2435
                     /locus_tag="VG274_06810"
     CDS             2133..>2435
                     /locus_tag="VG274_06810"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWE06400.1"
                     /translation="MRGEERRQRAMLVVIDPDKRAPKDHLVRRVKQLAEAALEELSPV
                     FDEMYSAVGRPSIPPGRLLKASLLMALYTVRSERMFCERLDYDLMFRWFLDLEMDEP"
BASE COUNT          483 a          725 c          762 g          465 t
ORIGIN      
        1 atctttcgcc ttcggccgaa ttgcgaccac tcggccaaga gcggccgacg ccgccgcgat
       61 cgtcgagatg gcggaaaaat ccaaaggcgc gcccatacac tcggccacat cgacggcgaa
      121 cggacgcacg accgcaggta gcatttccgc cgccatcggc ggcgccggcg gaaacgccgg
      181 ttcgatgcgc tcaggctcgg gccagtcttg ctgctcagcg tccggctcat cggccgcggt
      241 tgaatcgacg cgctcccaca agagataggc gatgctttcg caacggtctc gcgcttccgc
      301 atcgctcaag tcgtccggcg cccggtaacg atcgtcatgc gccatctgct cggtgagctt
      361 ttcgaggatt tcaggaacgg ggcaatttcg cagatagagc gcctgtgcaa actcaaaaac
      421 gagaagttcc caagccttca acgcgtgagc gtcgggatct gattgacgag attttccgtt
      481 ggcgcttacc gaagagaagt tgtttgagtt acggaagtca gatttagtat gatccacttg
      541 tcgcacacct gcgaagtgtt acgacgcacg ctcgtttaca cgctacgaag cgtccagttt
      601 ttgagcccgc atcggagtga tggccggtgc gggttccatc tcacccttta gattcagtct
      661 gcggtccgtc aggcgccggg ccgctcgaac agcggcgcca catcctcgat gcgaaaagct
      721 cggatgcgcg gagtgaccgc gatcggctga ggcagcttgc ccgccttaat caaccgccaa
      781 atcgtggctc gatgaacgtc aagccggcgc gcaagctcgg gcaactggat cagccgcggc
      841 agttcatgtt cgtgtttggt gctcatagcc agatgttagg gatcgcggcg catcgcgtca
      901 gaacgtcggc ctgcgaccgg cgcgatttca tttacaagcg cggagcgaag tcccttaatt
      961 tcagggcaat ttgcccgaga tttgccgcgt ccgagatgcg acgccgcgtg cgagatatag
     1021 tcccgaagtg acgctacgcg actttgctaa gcttgtggcg tgatgctcga tgcgctcatc
     1081 gccgcgattc agacgctcga tcccgccgat gttccggcgg ttatgatggc gctgtcggcg
     1141 cgtctgctgg cggcgaagtc catagcgccg gccgaatccg cgcccgattc cgaggacgtt
     1201 aatctgacca tcgcggaagc cgcggcgctt ctcagacgct cgggaaaatg ggtttacagg
     1261 catcgcacca gccttccgtt catccgcaag atcgggccgc gctcttacat ctgctcgaaa
     1321 cgcgccctcg atcgctggct cgcccgcaga caatcgctgt aacattcgag ttgacaatat
     1381 agcgcatgac gcgctacagt cgcgtcgtga tcgagagctt catccacaag gggctgaagg
     1441 agctgttcga aaccgggaag agcgccaagg ttcccgccca cctgaagcaa cggtccgatg
     1501 acgtgttgca ggcgctcgac gccgcggcca gcctgaaaga cctgaacctg ccagggtttc
     1561 aactccaccc gcttcacggc agtccagtcc gccacagcat ccacgtgagc ggccaatggt
     1621 gcatcacgtt cgaatggcag gcgccgcgcg cctaccgggt ggacctcgaa caatatcact
     1681 gaggtgtttc tgacatgagt gatgaatatg tcgtcaatcg cgagcgcatc acccgcgcgc
     1741 cggcgcatcc gggcgttttc ttcgaacgca atatactgcc cgagtttttg cgccagcgcc
     1801 gaacgatagg agagatcgcg acgctgctgg gcgtcgcccg ccagacgctg catcgcgtaa
     1861 tggccgggca gactgcggtc acgccggaca tggccgtgcg actcggcaag ctgtgcggca
     1921 acgggccgga tctgtggctc aacatgcagg cgcgctatga cgcgtgggaa gcaacgcggc
     1981 ggctgggcgc ggagctgaag aagattccga cgctgacgga ctaaggaggg cgaccataat
     2041 gaagcgcact agcaggttgt tgataaaggc ctgaatttcg gttcggcata gttcgtcgaa
     2101 gtgagtaatc cagtcggcgg aggaacgggc gcatgcgcgg cgaggagcgg cggcagcggg
     2161 cgatgctggt ggtgattgat cccgacaagc gggcgccgaa agaccatctg gtcaggcgcg
     2221 tcaagcaact ggccgaagcg gcgcttgaag agctctcgcc agtgttcgat gagatgtata
     2281 gcgccgtggg acggccgtcg attccgcccg ggcggctgct caaagcctcg ctgttgatgg
     2341 cgctgtacac ggtgcgcagc gaacgcatgt tctgcgagcg gctcgactac gatctgatgt
     2401 tccgctggtt cctcgatctg gaaatggacg agccc
//
LOCUS       DASZMC010000333         4162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3696182,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000333 DASZMC010000000
VERSION     DASZMC010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3696182"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..621
                     /gene="greA"
                     /locus_tag="VG274_06815"
     CDS             148..621
                     /gene="greA"
                     /locus_tag="VG274_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWE06401.1"
                     /translation="MERIPMTAGGYKTLEDEINHLKTVERPATIRLIEEARAHGDLSE
                     NAEYHAAKERQAFIEGRVVELEDKLGRAEIIDISKLSGNSVTFGATVTLVDEDTRAKK
                     KYQIVGDFEANARNGRISISSPIARALIGKTKGDSVEVTAPGGPRSYAILKVEFV"
     gene            complement(654..1448)
                     /locus_tag="VG274_06820"
     CDS             complement(654..1448)
                     /locus_tag="VG274_06820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWE06402.1"
                     /translation="MCQPAFDPRRFRANVPFYARFRLGYPDTLIDRIAAHAGLSQGDP
                     VMDLGAGPGLLAIPFARAGMAVTAIDPEPDMLSELRAAASEAGVAVDILEGSSFAMPR
                     GIGPFRLVTIGRAFHWMDRSAALQMLDPLVLPGGTVTLVHDDHPATVENAWRSLLREL
                     ADKFGRAEAPHLRAIKAPEYRTHESVLLGSPFCVLERLGVIVRRSLTADDIVGLGFSL
                     SALSPEKLGVRAPVFEAELREALSRLSPSGRFTEIAELSALLAKRL"
     gene            1517..2218
                     /locus_tag="VG274_06825"
     CDS             1517..2218
                     /locus_tag="VG274_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWE06403.1"
                     /translation="MATIALVDDDRNILTSVGMLLEQEGYHVRTYADGASALTALQAT
                     PPDLAILDIKMPRMDGLELLRRLRQGADLPVIFLTSKDEEIDELMGLNAGADDYVRKP
                     FSQRLLLERVKAVLRRADARKNGAGTTEPRREALVRGKLGLDPQRHDCTWDGKPVRLT
                     VTEFLILQCLAQRPGFVKSRDNLMDAAYDDQVYVDDRTIDSHIKRLRKKFKAVDDDFD
                     AIETLYGVGYRYRES"
     gene            2169..3830
                     /locus_tag="VG274_06830"
     CDS             2169..3830
                     /locus_tag="VG274_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HWE06404.1"
                     /translation="MRSKPFMALDIGIANPDAKRGVTLPGGTSRPLRRRVPGLSWRII
                     LANTAALAILIGGTLLVETSRQGLVDERVEEIRQEASIVASTLAEYATRERTRAIAIG
                     QAEPLLRQLIAPTNLRARLYATDGSLEIDTRDLLARNVVQTVELPPFDLRHRLEALAT
                     RVYDGVMGVRPFTSMAPYLETGGNGTVYSEVRRALNGEPASARRLDAQNRLVLSVAVP
                     IERFRTIYGVLMVTTEGGDIDDILRQERASLIGIALVAVVVMVISSFYLSGAIARPVR
                     QLAAAADRVRSGRLAREAIPLMPERNDEIGDLADSLRAMTRALYARIDAIERFAADVA
                     HELKNPLTSLKSAVEMLVRTGNEASRERLIAIVGNDIARIDRLITDISDASRLDAELS
                     REQIRPVDLAHFLETVVEIYGLVPLPRGVNLALTVKLPLGAKVLGQDERLGQVFRNLI
                     DNAVSFSPEGGIVAIVAENRDAYARVAVEDDGPGIPAEMLETIFDRFYTERPAEHGFG
                     RNSGLGLSIARQIAEGLGGRIWAENRSENRSGARFVVELPLAGWA"
     gene            3847..>4162
                     /locus_tag="VG274_06835"
     CDS             3847..>4162
                     /locus_tag="VG274_06835"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06405.1"
                     /translation="MTSSINIHASCVLLGRAGAAFGAPDHAGILLLGGSGAGKSDLAL
                     RLIAQGSVLVADDRCEISADDETLWARPPRALAGLIEVRGVGISALPFASEARIRLAV
                     RLV"
BASE COUNT          815 a         1287 c         1320 g          740 t
ORIGIN      
        1 ttaatttgaa gcgcgctcgt tgaaccaaac gacgagattg cgtagacttg acaggcactt
       61 aaaggaaagc ttgcaaaact ccggaccggc cgtgcccctt gggcaggccg agcggagctt
      121 ttttgccggt cgcgggatcg agaggccatg gaacgaatac caatgacagc cgggggctac
      181 aaaaccctcg aggatgagat caatcacctg aaaaccgtgg agcgcccggc gacgatccgt
      241 cttatcgagg aggcgcgcgc gcatggcgat ctctccgaga acgccgaata tcacgcggcg
      301 aaggagcgtc aggcgttcat cgagggccgc gtcgtcgagc tcgaggacaa gctcggtcga
      361 gcggaaatca tcgacatctc aaagctctcc ggcaattccg tcacgttcgg cgcgacggtg
      421 acgcttgtcg acgaagatac ccgcgccaaa aagaagtatc agatcgttgg cgacttcgag
      481 gcaaacgcca ggaacggacg aatttccatt tcctcgccga ttgcacgtgc gttgatcggc
      541 aaaacgaaag gcgattccgt tgaggtcacc gcccccggtg gaccccgctc ctacgcgatt
      601 ctgaaggtcg aattcgtatg agcccggcct cgccgcgaac tggggcccgc gaatcacagg
      661 cgctttgcca acagggcgga gagttccgcg atctcggtga atcgtccgct cggcgataga
      721 cgagaaagcg cttcgcgcaa ctccgcttcg aagaccggcg cgcgcacgcc cagcttttcc
      781 ggtgagagtg ccgacaggga aaatccgagg cccactatgt cgtcagcagt gaggctgcgc
      841 cggacgatca cgccaagcct ttcgagcacg caaaacggcg aaccaagaag gaccgattca
      901 tgcgtgcgat attccggagc cttgatcgcg cgcagatgag gcgcttcggc tcgaccgaat
      961 ttgtcggcca gttcgcgcaa caggctgcgc cacgcgtttt ccacagtggc tggatggtcg
     1021 tcatggacga gagtgacggt cccgcccggc agaaccagcg ggtcgagcat ctggagcgcg
     1081 gcagagcggt ccatccagtg aaatgcccgc ccaatggtca ccagcctgaa agggccaatc
     1141 ccgcgcggca tggcgaagga actgccttcc agaatatcca ctgcaacccc cgcctcggac
     1201 gcggcggcgc gcaattcgga cagcatgtcc ggctcggggt cgatcgcggt gaccgccatt
     1261 ccggcgcgcg cgaacggaat cgcgagcagg ccggggccgg ctccaagatc catgaccgga
     1321 tcgccctggc tcaggccggc atgggccgcg atccggtcga tgagcgtatc gggatagccg
     1381 agccggaagc gcgcgtagaa aggaacattg gcccggaagc ggcgcgggtc aaaggctggc
     1441 tggcacatct gaggctcgtg cgaattgggt ctcggacgac tatactagcc acggtcaaca
     1501 ccaactggac aatgatatgg cgacgatcgc actcgtcgac gacgacagaa acatcctgac
     1561 ctcggtcggc atgctcctcg agcaggaagg ttatcacgtt cggacctacg cggacggtgc
     1621 ctcggcgctg accgccttgc aggcgacgcc gcccgatctg gcgatcctcg acatcaagat
     1681 gccgcgcatg gacggtctgg aactgctgag gcgcctgcgg cagggcgccg atctgccggt
     1741 aatttttctg acatccaagg acgaggaaat cgacgaattg atgggactga acgccggcgc
     1801 cgacgactac gtacgcaaac cattctcgca gcgcctcctg ctcgagcgcg tgaaagccgt
     1861 attgcggcgt gccgatgccc gcaagaacgg ggctgggacc accgaacccc ggcgagaggc
     1921 gctggttcgg ggcaagctgg ggctcgatcc ccagcggcac gactgcacat gggacggcaa
     1981 acccgttcgg ctgaccgtca ccgagttcct gattctgcag tgtctggccc agcgtcccgg
     2041 cttcgtaaaa agccgcgaca atctcatgga cgctgcctat gacgatcagg tctatgtcga
     2101 tgaccgcacg atcgacagcc acatcaagcg gctgcgcaaa aagttcaagg cggtcgatga
     2161 cgatttcgat gcgatcgaaa ccctttatgg cgttggatat cggtatcgcg aatcctgacg
     2221 cgaaacgcgg cgtgaccctc cccgggggaa cttcgcgacc gctgcgccgc cgtgtgccgg
     2281 ggttgtcgtg gcgtatcatt ctcgcaaata cggcggcact cgcaatcctg atcgggggaa
     2341 cgctgctcgt cgagacgagc cggcaggggc tcgtcgatga acgagtggag gaaattcgtc
     2401 aggaagcttc gatcgttgcc agcacgcttg ccgaatatgc gactcgcgaa agaacgcgtg
     2461 cgattgcgat cggtcaggcc gaaccgctgc tgcgtcagct catcgctccc acaaacctcc
     2521 gcgcgcggct ctatgccaca gacggcagcc tcgagatcga cacgcgcgat ctgctcgcgc
     2581 gcaacgtggt gcagactgtc gaactgccgc cattcgattt gcgccaccgc ctggaggcgc
     2641 tggcgacacg cgtctacgac ggcgtgatgg gcgtgcgtcc attcacaagt atggcgccct
     2701 atctggagac cggcggcaac ggcaccgtct atagcgaggt acgccgcgcg ctcaatggcg
     2761 agccggcttc cgccaggcgc ctcgatgcgc agaacaggct ggttctctcg gtcgcagttc
     2821 caatcgagcg tttccgcaca atctacggcg ttctcatggt cacgaccgag ggcggggaca
     2881 tcgacgatat cctgcgccag gagcgcgcca gcctgatcgg catcgcgctt gtcgctgtcg
     2941 tggtgatggt catttcctca ttctatctgt ccggcgcaat cgccaggccc gtgcgccagc
     3001 tcgccgccgc cgccgaccgc gtgcgtagcg gccgtctggc gcgcgaggca atcccgctca
     3061 tgcccgagcg caatgacgaa atcggcgatc tcgccgacag tcttcgcgcc atgacgcgcg
     3121 cgctctatgc gagaatcgat gcaatcgagc gcttcgcggc cgatgtcgcg cacgagctga
     3181 agaacccgct tacttcgctc aagagtgccg tcgagatgct tgtccgcacc ggcaatgaag
     3241 cttcgcgcga acggttgata gcgatcgtcg gcaacgatat cgcgcggatc gacaggctga
     3301 tcacggacat ttccgatgcg tctcgcctcg atgcggaatt gagccgcgag cagattcgcc
     3361 ccgtcgacct ggcgcacttt ctggagacgg tcgttgaaat ctatggcttg gttccacttc
     3421 cacgcggagt gaacctcgcg ctcacagtca agctcccctt gggcgccaaa gtgctgggac
     3481 aggacgagcg gctgggccag gttttccgga atctcatcga caacgccgtt tcgttcagtc
     3541 ccgaaggtgg aatcgtggcg attgtcgccg agaaccgcga cgcatatgcc cgtgtcgccg
     3601 tcgaggatga tggccccggg attccggcgg agatgctcga aacaatcttc gaccgcttct
     3661 acacggagcg gccggccgag cacggctttg gaaggaattc gggtcttgga ctttccatcg
     3721 cacgtcagat cgcggagggc ctgggcgggc gcatctgggc cgagaatcgc tccgaaaacc
     3781 gatcgggcgc tcgcttcgtg gtcgagcttc cgctcgccgg atgggcgtag cgatgacttt
     3841 tcgctcatga cttcgtcgat caacattcat gcgagttgcg tgctgcttgg ccgtgcggga
     3901 gcggcgtttg gcgcaccaga tcacgccggc atcctgctgc tgggcggaag cggcgcggga
     3961 aaatcggacc tcgcgctgcg cctcatcgca cagggatcgg tgctggttgc cgatgatcgc
     4021 tgcgagatct ctgcggatga cgaaacgcta tgggcacggc cgccgcgcgc actcgcagga
     4081 ttgatcgagg tccgtggcgt cggcatttca gcgctcccgt tcgcttcgga ggcgcgcatt
     4141 cgtctcgcgg tccggctcgt cg
//
LOCUS       DASZMC010000334         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1264721,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000334 DASZMC010000000
VERSION     DASZMC010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264721"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VG274_06840"
     CDS             <1..287
                     /locus_tag="VG274_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06406.1"
                     /translation="GIGRLGSGDRAHGRGRGPGAANAGSGEMIVLRRMSLLGFVLVAP
                     PAMTALLYAMSEVLLVATHRVIAPPVLWYAAAIELPVFLLFAVLEAKNRW"
     gene            314..1432
                     /gene="ald"
                     /locus_tag="VG274_06845"
     CDS             314..1432
                     /gene="ald"
                     /locus_tag="VG274_06845"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWE06407.1"
                     /translation="MRIGVPKEIKNREYRVGLTPAAARELVSRGHEVIVETKAGDTIG
                     LSDDMYVRAGARIFTTAAEVFGAADMIVKVKEPQAAEIKRLRKGQTLFTYLHLAPDSE
                     QTRGLLETGAIAIAYETVTDSRGGLPLLAPMSEVAGRMSIQAGAHSLEIAQGGRGMLL
                     GGVPGVAAAKVVILGGGVVGTNAARMAMGLEAHVTILDVSLPRLNELDLRFGGMLNTI
                     YSTIDSIEESVVSADLVIGGVLLPGAAAPKLITEKMVKQMKKGSVIVDVAIDQGGCAE
                     TSHATTHDAPTYLVHDVVHYCVANMPGGVARTSTFALNNATLPFTLALAEFGPEQAMK
                     KNPHLRAGLNVYKNTLTYKAVAEAQNLPYRSAEDVLGA"
     gene            complement(1586..2353)
                     /locus_tag="VG274_06850"
     CDS             complement(1586..2353)
                     /locus_tag="VG274_06850"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE06408.1"
                     /translation="MELFDLTGKVALVTGSTRGIGEAIVHRLAEHGAKVVVSSRKADA
                     CERVAAAINKARGGDVALPVPCNINYKEQLQALVAATRAKWQRIDVLVCNAAINPYFG
                     PQMEMTDDAFDKIMGANVRSNHWLCQLVIPEMAERREGSVVIVSSIGGLRGSPVIGAY
                     CISKAADMQLARNLAVEYGPQNVRVNCIAPGLVKTDFARALWENPEILKRSTSGAPLR
                     RIGEADEIAGAAVFLASKAGAFMTGQTIVIDGGATIA"
     gene            2448..3098
                     /locus_tag="VG274_06855"
     CDS             2448..3098
                     /locus_tag="VG274_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWE06409.1"
                     /translation="MSKSILIAVAIAMLAGCMTEPPPERAYPPEFAAPPPPRAAKAPA
                     ETVAPAPGLHQAEIKPLGRGLLAAQAAGGYMDGQEKELRAGLRGTGVTVSRMGDSLVL
                     NLRADTVFGGKTAEISEQGRRIVDKISDSIRKFDSTQIVINGFTDTTGTEARNLKVSQ
                     ERADAVARAFAEDGVDSHRVAAKGFGDEILKIPTGPDVSEPRNRRLEIRITPRVKT"
     gene            complement(3112..3600)
                     /locus_tag="VG274_06860"
     CDS             complement(3112..3600)
                     /locus_tag="VG274_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HWE06410.1"
                     /translation="MTAKNAYDFAFRSIDGGDLPVSRFKGKALLVVNVASQCGLTPQY
                     SGLEALWTGRKNEGLVVLGVPANDFGAQEPGSEAEITAFCETRYGVDFPMTAKEHVIG
                     PDAHPLYKWVAEELGEAAAPKWNFHKYLFGRDGTISGTFGSRTPPDAPELTSAIDMAL
                     KS"
     gene            3698..>4176
                     /locus_tag="VG274_06865"
     CDS             3698..>4176
                     /locus_tag="VG274_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06411.1"
                     /translation="MPKAPIKTGEIQVVKPTAAFEAAESPVILSGLGAIADSYDALLC
                     DVWGVLHDGRSAHVCAMDALKRFRRLRGPVILLSNAPRPAEDVQQQFEHLGIAEDSYD
                     AIVTSGMLTRNELARRAADKELKLLHIGPERDRGIFAGLPIACVEADRAEAVVCTGL"
BASE COUNT          779 a         1332 c         1313 g          752 t
ORIGIN      
        1 ccggcattgg tcgcctcgga agcggagatc gcgcgcatgg ccgagggcgt gggccgggtg
       61 ctgcaaacgc tgggtcggga gaaatgatcg ttttgcgcag aatgtccctg ctcggcttcg
      121 tcctggtcgc gccgcccgcc atgaccgcgc tcctctatgc gatgagcgaa gtcctcctgg
      181 tcgccacgca ccgcgtcatc gctccgccgg ttctgtggta tgcggcggca atcgaactgc
      241 ccgtcttctt gctgttcgcg gttctcgaag caaagaaccg ctggtaatcc aacttcactc
      301 tcgcgaggca tgcatgcgca tcggtgttcc aaaggaaatc aagaatcgcg aatatcgcgt
      361 gggtctcacg cctgcggccg cgcgcgaact cgtgagccgc ggccacgaag tcatcgtgga
      421 aacgaaggcg ggcgacacca tcggattgag cgacgacatg tatgtgcggg ctggcgcgcg
      481 gatattcacc accgcagcgg aagtcttcgg cgcggcagac atgatcgtga aggtaaagga
      541 gccacaagcg gccgagatca agcggctgag aaaaggccag acgctgttca cctatctcca
      601 tctcgcgcca gattccgaac agacgcgcgg gctgctcgag accggcgcga tcgccattgc
      661 gtacgagacg gtcaccgact cgcggggcgg gttgccgcta ctggccccca tgagcgaagt
      721 ggcgggccgc atgtcgatcc aggccggcgc gcactccctt gagatcgcgc aaggaggccg
      781 cggcatgctg ctcggcgggg ttcccggcgt ggcggcagcg aaggtggtga ttctgggcgg
      841 cggcgtcgtc ggcaccaatg cggcgcgcat ggcgatgggg ctcgaggctc acgtcaccat
      901 cctcgacgtc tcgcttccgc ggctcaacga gctcgacctg cgattcggcg ggatgctgaa
      961 cacgatctat tccacgatcg attcgatcga ggagagcgtc gtcagcgccg atctcgtgat
     1021 cggcggcgtg ctgctgcccg gcgcagcagc gccgaagctg atcaccgaga agatggtcaa
     1081 gcagatgaag aaaggctcgg taatcgtcga cgtggcgatc gaccagggtg gctgcgcaga
     1141 aacctcgcac gccaccacgc acgatgcgcc gacctacctg gtgcacgatg tggttcatta
     1201 ctgcgtcgca aatatgcccg gaggcgttgc gcgcacttcg accttcgcgc tcaacaacgc
     1261 gaccttgccg ttcacgctgg cgctcgccga gttcgggccc gaacaggcga tgaagaaaaa
     1321 tccgcatctg cgcgccggtc tcaacgtcta caagaatacg ctcacctaca aggccgtcgc
     1381 cgaagcgcag aacctgccct accgcagcgc cgaggacgtt ctgggggcat agtaccggcg
     1441 accgcagaga aacagattaa caatgaggca gcgaggcagt gagaactatt gcgcgccaga
     1501 ggcgcgcgat tctcttctcc ccgtctcact gcctcattgt gaatcccttt tcgccgcggc
     1561 tcttcgcctc cgcctgcaaa agcgctcacg cgatggtcgc cccgccgtct atcacgatcg
     1621 tctggccagt catgaaggcg cctgctttcg aggcgagaaa gacggctgcg cctgcgatct
     1681 cgtcggcctc gccgatgcgt cgcagcggcg cccccgaggt cgagcgtttc aggatttcgg
     1741 gattttccca cagcgcgcgc gcgaagtccg ttttgacgag tcccggtgcg atgcagttca
     1801 cgcgcacgtt ctgcggcccg tattctacgg cgagattgcg cgcgagttgc atgtcggccg
     1861 ccttggagat gcagtacgcg ccgatcacgg gagagccgcg cagccctccg atcgaggaga
     1921 cgatgacgac ggagccctcg cggcgttccg ccatctcggg aatcacaagc tggcaaagcc
     1981 agtgattcga gcgcacattg gcgcccatga tcttgtcgaa ggcatcgtcg gtcatttcca
     2041 tctgcggccc gaaataaggg ttgatggcgg cgttgcagac gagcacgtcg atccgctgcc
     2101 attttgcgcg cgtggccgcg accagcgcct gaagctgctc cttgtaattg atgttgcagg
     2161 gcacgggcag cgcgacatcc ccgccgcgcg ccttgttgat ggccgctgcc acccgctcgc
     2221 acgcatcggc cttgcggctg gagacgacga ctttcgcgcc atgttcggcc agccggtgaa
     2281 cgatggcttc gccgattccc cgcgtggaac cggtcaccag cgcgaccttg ccggtcagat
     2341 caaaaagctc catcttgcct tcccttccgg aaacaatttg cctgcagctt cattgttagt
     2401 ccgggccgga agccgggaca aaccggcccg catccccgga gaacccgatg tcgaaatcga
     2461 tcctgatcgc cgtggcgatt gcaatgctgg cgggctgcat gacggaaccg ccgcctgagc
     2521 gcgcctatcc ccccgaattc gcggctccgc caccgccgcg ggcggcgaaa gcgccggctg
     2581 agactgtagc gcccgccccc ggtctgcatc aggccgaaat caaaccgctt ggccggggcc
     2641 tgctcgcggc gcaggccgcg ggggggtaca tggacgggca ggaaaaggaa ctccgcgccg
     2701 gcctgcgcgg caccggcgtc accgtcagcc gcatgggaga cagcctcgtc ctcaatttgc
     2761 gcgccgacac ggtgtttggc ggcaaaacgg ccgagatcag cgaacagggg cggcgcatcg
     2821 tcgacaagat ttccgacagt atccggaaat ttgacagcac ccagattgtc atcaatggct
     2881 tcacggatac gacgggcacc gaggcgcgaa acctgaaagt ttcgcaggag cgcgccgacg
     2941 ccgtcgccag ggctttcgcc gaggatggcg tagattcgca tcgcgtcgcc gccaagggct
     3001 tcggcgatga gatcctcaag attccgaccg gccccgacgt gagcgagccg cgcaaccgcc
     3061 ggctcgaaat ccgcatcacg ccacgtgtga aaacctgacc gacgccgacg ctcagctctt
     3121 cagtgccatg tcgattgccg aagtcagctc gggggcgtcg ggcggggtgc gcgagccgaa
     3181 cgttcccgag atcgttccat cgcggccgaa gaggtatttg tggaaattcc atttcggcgc
     3241 ggcggcctcg ccgagttctt ccgccaccca tttgtagagc ggatgggcat cgggcccgat
     3301 cacgtgttcc ttcgcggtca tcgggaaatc gacgccatag cgcgtctcgc agaaagccgt
     3361 gatttcggct tcgcttcccg gctcctgggc gccgaaatca ttcgccggca caccaagcac
     3421 cacgagtcct tcattcttcc gccccgtcca cagcgcttcg aggccgctat attgcggagt
     3481 caaaccgcat tggcttgcga cgttcaccac gaggagcgcc ttgcctttga atctcgaaac
     3541 cggcaaatcg cctccgtcga tggagcggaa agcgaaatcg taagcgttct tcgcggtcat
     3601 gatgatgcgt cctcttcgga gttccgccga acttagtgca agattggaca agattgaatg
     3661 acgattgtgt tttccgtccg taccaaagca acatgtcatg ccgaaagcgc cgattaagac
     3721 cggcgagata caggtcgtga agccaacggc agctttcgaa gccgccgaat cgccagtgat
     3781 tctctctggg cttggcgcga tcgcggattc ctacgacgct ttgctgtgcg acgtgtgggg
     3841 ggtgctgcat gacgggcgca gcgcgcacgt ttgcgccatg gacgcattga agcggttccg
     3901 ccgcctgcgc ggtccggtca tcctgctttc gaatgcgccc aggcccgccg aggacgtgca
     3961 gcagcagttc gagcatctcg gcatcgccga agacagctac gacgcgatag tgacatcggg
     4021 catgctgacg cggaacgaac tcgcccgacg cgcggcggac aaggagctga agctgctcca
     4081 tatcggtccc gaacgcgacc gcggcatttt cgcgggtttg cccatcgcct gcgtcgaagc
     4141 ggatcgcgcc gaggccgttg tgtgcaccgg actcta
//
LOCUS       DASZMC010000335         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2614537,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000335 DASZMC010000000
VERSION     DASZMC010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2614537"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1257)
                     /locus_tag="VG274_06870"
     CDS             complement(37..1257)
                     /locus_tag="VG274_06870"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE06412.1"
                     /translation="MTDTVYRRRDFWSFVSGRFVATVAMQVQSVAIGWQIYDIERTPF
                     ALGLVGLCQFAPMFLLTLPAGEITDRYDQRRVFAAALMLQGVCAALFMAFSLTMPRNA
                     LPYYGVLILFGAARGFSGPAGSSLLPFLVPPEKLARSIALNSSGFTTAVIAGPALGGL
                     LFLVGPVATYGLCMAGFLLCALLIATLGGRRAQKTASTASRLERVIEGIRFVRDRPIV
                     LGAISLDLFAVLLGGAVALLPVYARDILHTGPEGLGMLRSAPAVGAAVTALALARKPI
                     DRHTGPIMFGCVALFGTATIAFGLSTNFIISLIALFVTGASDMVSVFVRTALVNFATP
                     DAMRGRVGAVNMLFIGASNELGEFESGFTAALFGTVPAVVIGGLGTLGVVALWMKLFP
                     PLRTVDRLSDASRA"
     gene            complement(1266..1802)
                     /locus_tag="VG274_06875"
     CDS             complement(1266..1802)
                     /locus_tag="VG274_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE06413.1"
                     /translation="MIPRLETERLILRGWRPEDFEPLALIHGDPETMTFVGGVQERND
                     AWRSLASLAGHWALRGYGKWAVERKSDGALLGRVGMINPEGWPGLEIGWTLGKQYWGK
                     GYATEAAQAALDYAFLTQPMERMISCIDANNIASQAVAKRIGETKAESAALRIGGKEF
                     PVDIWAITRAEWTKRRAA"
     gene            complement(1908..2336)
                     /locus_tag="VG274_06880"
     CDS             complement(1908..2336)
                     /locus_tag="VG274_06880"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HWE06414.1"
                     /translation="MSLKPILLAAGILTTVTLPVGAADTATLTITVHNISDAGGDLRI
                     GVYDEANFALKGGVPVARKVTHARGPTMIITFDAIAPGTYGAKVLQDINQNGQFDIGL
                     KGVEPIGFSDDPQLKGGLPPFNDVKFAVAPGANSIDVTLH"
     gene            complement(2333..>2533)
                     /locus_tag="VG274_06885"
     CDS             complement(2333..>2533)
                     /locus_tag="VG274_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06415.1"
                     /translation="YKQWKASPQTFAPAYIALLQNGFDDTPVNLLKRFLRIDLDDEAT
                     LVANAQSVAEEKLAALEDHKSP"
BASE COUNT          544 a          783 c          771 g          435 t
ORIGIN      
        1 ccccagtccg catcgtggcg caggcggacg cgaagctcaa gcgcggctag cgtccgatag
       61 ccggtcaaca gtgcgcaaag gcggaaacag cttcatccag agcgcgacaa cgccaagcgt
      121 gcccagtccg ccgattacga ccgcaggcac cgtgccgaac agggcagccg tgaaacccga
      181 ctcgaactcg ccgagttcgt tggaggcgcc aatgaacagc atattgaccg cgccgacgcg
      241 cccgcgcatc gcatctggag tcgcgaaatt gacgagcgcg gtgcgcacga agacgctgac
      301 catgtcggaa gcacccgtga cgaacagcgc gatgagtgag atgataaaat tggtcgaaag
      361 accgaacgcg atggtcgctg tgccgaagag cgcgacgcag ccgaacatga tcgggccggt
      421 atgcctgtcg atcggcttgc gggcgagcgc caacgccgtc accgcggctc ccaccgccgg
      481 cgcactgcgc aacataccta acccttcagg accggtgtgg agaatatctc gcgcataaac
      541 cggcaggaga gccacggcgc cgccgagcag cactgcgaac agatcgagcg agatcgcacc
      601 taacacgatg ggccggtcgc gcacgaaacg gatgccttcg atcacgcgct ccaggcgaga
      661 ggcggtcgat gcggtcttct gtgcgcgcct tccgccgagc gtggcgatca ggagcgcgca
      721 gagaagaaat ccggccatgc agagtccata ggtcgcgacc ggtccgacaa ggaaaagaag
      781 tccgcccaac gctgggcctg cgatcaccgc cgtcgtgaag ccagaggaat tgagcgcgat
      841 agaacgcgcc agcttctccg gcggcacgag aaacggcagc agcgaacttc ccgcaggacc
      901 cgaaaacccg cgcgcggcgc caaacaggat cagcacgcca taatatggca gggcattgcg
      961 cggcattgtg agcgagaaag ccatgaacag cgcggcgcaa acgccctgca acatcagcgc
     1021 ggcggcgaac acgcgtcgct gatcgtaacg gtctgtgatc tcaccggcag gaagtgtcag
     1081 cagaaacatt ggcgcgaatt ggcaaagccc gactaggccg agcgcaaacg gcgtgcgctc
     1141 gatatcatag atctgccaac cgatggcgac cgattggacc tgcatagcaa cggtcgcgac
     1201 aaaacggccg gaaacaaagc tccagaaatc gcggcggcgg taaacggtat cagtcattgt
     1261 attggtcatg cggcgcggcg ctttgtccat tccgcgcgcg tgatcgccca gatatcgacg
     1321 gggaattcct tgccgccgat gcgcagcgcg gcgctctcgg ctttggtctc gccgatgcgc
     1381 ttcgccacgg cttgtgacgc aatgttgttg gcgtcgatgc aggaaatcat gcgctccatt
     1441 ggctgcgtca ggaatgcata gtcgagcgcc gcttgcgcag cctcggtcgc gtaacctttg
     1501 ccccagtatt gcttgccgag tgtccagccg atctcaagac caggccagcc ttcgggattg
     1561 atcatcccga cccggccgag cagagcgccg tccgatttcc gctccaccgc ccatttgccg
     1621 tagccacgca gcgcccagtg tccggcgaga cttgccaggc tgcgccacgc gtcattgcgc
     1681 tcctgcacac cgccaacgaa cgtcatggtt tcaggatcgc cgtggatcaa ggccagcggc
     1741 tcgaaatctt ccgggcgcca gccacgcagg atcagacgtt cggtttcaag gcggggaatc
     1801 atgggcactc aatagagcaa cgactccagt gtagccagat ttccatccag ttggcgccgc
     1861 gcttcagttc gattttcgca gcgtcgaagt ctggcggcgt ggacgcctta gtgcaatgtg
     1921 acatcaatgg aattggcacc cggtgcgacc gcaaatttca catcattgaa gggcggcaga
     1981 ccgcccttca actgagggtc gtcggagaag ccgatcggct caactccttt caggccgata
     2041 tcgaactggc cgttctggtt gatgtcctgc agcacctttg cgccgtaagt acccggcgca
     2101 atcgcatcga acgtgatgat catggtggga ccccgcgcgt gagtgacttt tcgtgccacc
     2161 ggcacgccgc ccttcagggc gaagttcgcc tcgtcgtaaa cgccaatacg aaggtcgccc
     2221 ccggcgtcgg aaatattgtg cacggtgatc gtgagcgtcg ccgtgtcggc ggcaccaacc
     2281 ggaagcgtga ccgtcgtcag aataccggcg gctaaaagaa tcggtttcaa actcatgggc
     2341 tcttgtgatc ctccagcgcg gccagctttt cttccgccac gctttgcgca ttcgcgacca
     2401 aggtggcctc gtcgtcgaga tcgatcctga gaaaacgctt gagcaggttg accggtgtat
     2461 cgtcgaaacc attttgcagc agcgcgatat aggcaggcgc gaaagtctgc ggcgatgctt
     2521 tccactgttt gta
//
LOCUS       DASZMC010000336         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2345387,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000336 DASZMC010000000
VERSION     DASZMC010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345387"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VG274_06890"
     CDS             <1..247
                     /locus_tag="VG274_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06416.1"
                     /translation="ATVIGFGLGFTGIGAIHMLVWSAVLNGIVAVPIMAMMMVIVTNS
                     RIMGRFKAKPRLIALGWIGTGLMALAVIALFWSLLAG"
     gene            complement(244..735)
                     /locus_tag="VG274_06895"
     CDS             complement(244..735)
                     /locus_tag="VG274_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06417.1"
                     /translation="MSSAFPDIAPSQPAPPPERAPPLGLFSGKPMSQWQLPPSVWGLP
                     DHSDASGDGEWLNFLAGIASRSPTQPEPPPQPDDSKSARYLSRRTVDQSRVPAVGQGA
                     PVTPLAPSSVDPNFSGGLGRLTALAGVDPQDPTQPAPPLDDQLRGFYRDDPVQPWFVQ
                     RPR"
BASE COUNT          302 a          474 c          532 g          305 t
ORIGIN      
        1 tgccacggtg atcggattcg gcctcggctt taccggaata ggcgccattc acatgctggt
       61 gtggagcgcc gtgctcaatg gcattgtcgc ggttccgatc atggcgatga tgatggtgat
      121 cgtgaccaat tccaggatta tgggaaggtt caaggcaaag ccgcggctga ttgctttggg
      181 ttggatcggc accgggctga tggcgctagc ggtgatcgct ctgttctggt cgcttttggc
      241 gggctagcgc ggcctctgaa cgaaccatgg ctgcacggga tcgtcgcgat aaaacccgcg
      301 cagttggtca tccagcggcg gcgcaggctg cgtcggatct tgcgggtcga cgcctgcgag
      361 agcagtgagc ctgcccaggc ctccggagaa atttggatca acagatgatg gagcaagcgg
      421 cgtgaccggc gcgccctgcc caactgcggg cacccgcgat tggtcgaccg tcctccggct
      481 caaatatcgc gccgacttgc tgtcgtcagg ttgcggcggc ggctcgggct gcgtcggact
      541 acgggaggcg atgcctgcca gaaaattgag ccattcgccg tcgccggacg catcggaatg
      601 atccggcagg ccccagaccg aaggcggaag ctgccattgc gacatcggct tgccgctgaa
      661 gagtccaagc ggcggggcgc gttccggcgg tggtgccggc tggctcgggg cgatgtccgg
      721 aaaagcagaa ctcaccctgc ggctttgcag ccgcgggtac gtgtcgggcc tcggcgcgcc
      781 cggcgtaatt tccgaccgat cgccgaaagc aggcggttcg cctgggaaat ctttcgcagt
      841 ggggcttggc aagatcgacc tcgatggcga cgctgttgcc gcaggcgtga tcagcctatc
      901 cccaacgaac gtgccaagaa atggcccacc tcgcttgccg gcaacgtcgg gctgaagcgc
      961 gagtgctgcc ggaggtgccg cttgcgccga ggtggacgcc cggccgagag cctcggatct
     1021 cctgtaattc tgcagcgcgc gcaagaccgg agaacgtgga ttgccaaaat cgccggccgg
     1081 ggcagagttc cagttgccga agcgactatt gaacgaatcc ggacgatcgg ccgcaggtag
     1141 caggacactg gccgccggcg cagagcccca ttttccgaaa cggtcgccga acgaatcctg
     1201 acgatccggg aaattcccga tgaagtatcc gtcgggtgaa tacatcgcgt ccggttgaaa
     1261 cgcgggtatt ggcctcgcgt gagtcatgga cgtgggcagt acaacatccg acgctggcgc
     1321 ccagtgttcg ctcccgaagc cggttccgac cggacgcggc acatccccag cggtcaggcc
     1381 cgttgatgcg gttgaatttg aattctgggt agctggagaa taaggcagtc cttgttcgcc
     1441 gatctgggaa tacgcctgca ctgttttcga ccagtttccg gaaagatcat cgccgacctt
     1501 gagaatttgt gaatcgtacg ggtgagcttg cggcccgaac aaatgctccc attcccgatc
     1561 cgataaatca ctcacccgcc cccactgggt cacgatagtg ccatacggcg agg
//
LOCUS       DASZMC010000337         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4277491,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000337 DASZMC010000000
VERSION     DASZMC010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4277491"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /gene="glmS"
                     /locus_tag="VG274_06900"
     CDS             complement(<1..1591)
                     /gene="glmS"
                     /locus_tag="VG274_06900"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWE06418.1"
                     /translation="MCGIVGIAGGEEAAPVILEALRRLEYRGYDSAGIATLAGGHIER
                     RRAAGKLGNLAAVLKADPLAGRTGIGHTRWATHGAPTESNAHPHATTRVALVHNGIIE
                     NFRELRDELAAKGHRFDSETDTEVAVHLVTDYLEKGHDAPHAAIAATQRLTGAYALAM
                     IFDGEESLVIGARKGSPLAVGYGAKESYLGSDAFALAPFTNRICYLEDGDVAVLRGPD
                     VSIFDAEGRPANREIRVVGGAEGLVDKGGFGHFMAKEIHEQPEVIAHTLAHYLDPAGP
                     RVALADARVSAALARAGRLTVSACGTAFYAGLVGKTWFEQLARIGVEVDVASELRYRE
                     PVYAKDGSALFISQSGETADTLAALRHAKANGQTTLAIVNVPESSIAREADIVLPTFA
                     GPEIGVASTKAFTCQLCVLAALAIAAGRVRNVLGADAERRLCKALLETPRHIVEFLKQ
                     ESAVRALGVEIAVARDVLYLGRGLSYPLALEGALKLKEISYIHAEGYAAGEMKHGPIA
                     LIDASVPVIVLAPFDALFEKTV"
     gene            complement(1595..>2470)
                     /locus_tag="VG274_06905"
     CDS             complement(1595..>2470)
                     /locus_tag="VG274_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HWE06419.1"
                     /translation="SDSARTGTLCNAGIFAAEAKAFFRWTAALGSDNAQREYYLTDVP
                     QLARRDGVACAVATVDEDDVMGVNSRSELLRAERCAQARLRESAIEAGVTLREPATVH
                     LSFDTVLESDVEVEPYVVFGPGVVVRRGARIRAFTHLEGAEVGRNAIVGPYARLRPGT
                     LLEESVHIGNFVEVKATRVGKAAKANHLTYLGDAVVGAGANIGAGTITCNYDGFDKHE
                     TEIGAGAFIGSDTALVAPVRVGAGAYIGAGSVITRDVSADALAVVRAEQVEKPGWAEK
                     FRTRKKAEKAAKEGS"
BASE COUNT          404 a          864 c          782 g          420 t
ORIGIN      
        1 aaaccgtttt ctcgaacagg gcatcgaatg gcgcgagcac gatgacgggc acgctggcgt
       61 cgatcagcgc gatgggtccg tgcttcatct cgcccgccgc atagccttcg gcgtggatgt
      121 aggaaatctc cttcagcttc agcgcgccct cgagtgcgag gggatagctg aggccccgcc
      181 ccagatagag cacgtcgcgg gcgactgcga tttcgacgcc gagcgcgcgg accgcggact
      241 cctgcttcag gaattcgacg atgtgccgcg gcgtctccag cagcgctttg caaagccgcc
      301 gctccgcatc ggcgcccagc acattgcgca cgcgcccggc cgcaatcgcc agcgcggcca
      361 gaacgcacaa ttggcaggtg aacgccttgg tggaagcgac gccaatctca ggcccggcaa
      421 aagtcggcag cacgatatcg gcttcgcgcg cgatcgaact ttccggcacg ttgacgatgg
      481 cgagcgtcgt ctgcccgttc gccttcgcat ggcgcaaggc cgccagcgtg tccgccgtct
      541 cgcccgattg cgagatgaag agcgcggacc cgtccttcgc atagacgggc tcgcgatagc
      601 gcagctccga ggcgacatcg acctcgacgc caatccgcgc cagttgctcg aaccaggttt
      661 tgccgaccag tcccgcataa aaggccgtgc cgcaggccga gacggtgagc cgcccggccc
      721 tggccaaagc ggcactcacc cgcgcgtcgg cgagcgcgac gcgcggtcct gcgggatcga
      781 gataatgcgc gagcgtgtgg gcgatcactt ccggctgctc gtgaatctcc ttcgccatga
      841 aatggccaaa gccgcccttg tcgacgagac cctcggcgcc gcccaccacg cggatttcgc
      901 gattcgccgg ccggccctcc gcatcgaaga tcgagacatc gggcccgcgc agcacggcca
      961 cgtcgccatc ttcgaggtag cagatgcgat tggtgaaggg cgccagcgca aaagcatccg
     1021 agcccagata ggattccttc gcgccgtagc cgaccgccag cggactgccc ttgcgggctc
     1081 cgatgacgag ggattcctcg ccgtcgaaga tcatcgccag cgcataggcg ccggtcagcc
     1141 gctgcgtcgc cgcgatcgcg gcgtgcggag catcatgtcc cttttcgaga tagtcggtga
     1201 cgagatggac ggccacttcg gtgtcggttt cggaatcgaa acggtgcccc ttcgcagcga
     1261 gctcgtcgcg cagctcgcgg aaattctcga tgatgccatt gtggacgagc gcgacgcgcg
     1321 tagtggcgtg ggggtgcgcg ttcgattcgg tgggcgcgcc atgcgtcgcc cagcgcgtat
     1381 ggccgatgcc ggtgcggccg gccagcggat cggccttgag caccgcggcg agattgccga
     1441 gcttgccggc cgcgcggcgg cgctcgatat gaccgcccgc cagcgtcgca atgcccgccg
     1501 agtcgtagcc gcgatattcg agccgccgga gcgcctccag aataacgggc gccgcctcct
     1561 ctcctcccgc aatgccgacg atcccgcaca tctgtcagct cccctctttc gcggcttttt
     1621 ccgctttctt ccgtgtacgg aatttctcgg cccagccggg tttttcgacc tgctccgccc
     1681 ggacgaccgc cagcgcatct gcgctgacgt ccctggtaat gacgctgccc gcgccgatat
     1741 aggcgcccgc gccgacgcgc accggcgcca ccagtgccgt gtcggagccg atgaaggcgc
     1801 cggcgccgat ctcggtttcg tgcttgtcga agccgtcgta gttgcaggtg atcgttcccg
     1861 cgccgatatt ggcgccggcg ccgacgaccg cgtcgcccag ataggtgaga tggttcgcct
     1921 tcgcggcttt gccgacgcgc gtggccttta cctcgacgaa attgccgata tgaacgctct
     1981 cctcgagcag tgttccaggc ctgagccggg catagggacc cacgatcgca ttgcgcccca
     2041 cttcggcgcc ctcgagatgc gtaaatgcac ggatgcgcgc acctctccgc accacgacgc
     2101 ccggcccgaa cacgacatac ggctcaacct ccacatccga ttccagaacc gtgtcgaagg
     2161 agagatggac ggtcgcaggc tcgcgcaacg tgacgcccgc ctcgatcgcc gattcccgca
     2221 atcgcgcctg cgcgcagcgt tcggcgcgca ggagctcgga ccggctgttg acccccatga
     2281 cgtcgtcttc atcgacggtc gccaccgcgc acgccacgcc gtcgcgcctg gccagttgcg
     2341 gcacgtcggt cagataatat tcgcgctgcg cgttgtcgct gccgagcgcg gccgtccagc
     2401 gaaaaaacgc cttcgcctcg gctgcgaaga ttccggcgtt gcacaacgtg ccggtgcgcg
     2461 cgctgtcgct
//
LOCUS       DASZMC010000338         5358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3991970,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000338 DASZMC010000000
VERSION     DASZMC010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3991970"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VG274_06910"
     CDS             <1..1485
                     /locus_tag="VG274_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06420.1"
                     /translation="ALNFQFAIGQGPGKFRGAIVENISILNDGVTQNLGHGPVALGFF
                     NTGINVTGCNYPYFDDVTINGCEGAGAVVDRSDGDPAYAMQTALVVDDCYDPKLQDCR
                     FWHGQVGVSYQNNARGEDPAAHVQAFQMLGCKLNGFQTGLKFSNNGVVFPHTVITGNE
                     GTYRDVGFDIGDPFKIMLTDNQFSSDGDGTYTRNGGVYYDMWFKHANVVTIVGSGFNT
                     KSDPDRVCIYIDAVTDTGGENTHGGKHFLVAENQFGDPVGGSPFGTNAVKVTAGATDI
                     QIGHNDYAGTFSDLLVNDASGNAVIWNASVSQAQTLVAEVQPVRSGSTPTNAGPIIDL
                     YQNVHAGGGANDTIGALQMSANLEFGETKATFAAITASIQSAVAEALMGILNISIVAG
                     AYLQNVLTLSLSNINMGTTGTTSVTPAVDNAITLGSAAQRWSKTYAVARYFSGGVFDS
                     TGSGSPDGVVTAPVGSTYRNTGGGSMPTFWVKQTGTGNTGWVGK"
     gene            complement(1540..1616)
                     /locus_tag="VG274_06915"
     tRNA            complement(1540..1616)
                     /locus_tag="VG274_06915"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1580..1582),aa:His,seq:gtg)
     gene            1858..3390
                     /gene="hutH"
                     /locus_tag="VG274_06920"
     CDS             1858..3390
                     /gene="hutH"
                     /locus_tag="VG274_06920"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HWE06421.1"
                     /translation="MANLDIDPGRATLSLWRRVLETAAPISLAPAAWPIIERARHTVE
                     DALAAGRVLYGVNTGFGKLAQMRIAPGEIKTLQRNLVLSHSAGVGELLPDALVRLVMA
                     LKVASLGRGFSGVRRILVERLLQLLNANVLPEIPSQGSVGASGDLAPLAHLAAVLIGE
                     GRARIAGRSMPAGDALAQAGIAPIELEAKEGLALLNGTQVSTALALSALFAAEANLEA
                     ALVAGALSTDAVKGSDTPFDARIHDARGQPGQIEAAHALRTLMAGSEIRASHRDCPRV
                     QDPYSIRCQPQVLGPCLDILRNVAGVLAIEANAATDNPLVFENGDTLSGGNFHAEPVA
                     LAADTLALAIAETGALSERRIALLIDSALSGLPAFLAHDGGVNSGFMIAHVTAASLAS
                     ENKSLAHPASVDSLPTSANQEDFVSMATFAARRLGAMNANTANILAVELLAAAEGIEF
                     HRPLKSSAALETAHAALRARVPRFDRDRMMSPAIAAASELVSGRAFEKLLPDDFRLPS
                     QR"
     gene            3645..3992
                     /locus_tag="VG274_06925"
     CDS             3645..3992
                     /locus_tag="VG274_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HWE06422.1"
                     /translation="MTWPMIAPILMLSVSNIFMTFAWYGQFKFPAASLWIVIPVSWGI
                     AFFEYCFAVPANRLGHLVYSAAQLKTMQEIITLLVFGVFAVTYLGDTIRWNHFAAFAC
                     LVAAAFFSFNKWS"
     gene            4533..>5358
                     /locus_tag="VG274_06930"
     CDS             4533..>5358
                     /locus_tag="VG274_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06423.1"
                     /translation="MPDLTIPGARVSAIPPSLVTRVGQAVRYAITGVTPATWFGPMQP
                     LKPFAPEGTGGRAFDYPVGYNLRYQPRADEPISFVDLRALADGCDLLRLVIETRKDQI
                     EGQTWDVRPRKLPNGAHPAASAFATEIAKAKAFFAYPDRELDLASWIRALAEDMFVTD
                     AVSIYLRPDRRQGIYGIEVIDGATIAPRIDAWGRTPQAPDVAYQQILHGVPAADFSRD
                     QLVYFPRNRRPGHVYGYSPVEQVIVTANTLIRRSLHQLAYYTEGNLVDGLFTSPPEW"
BASE COUNT          945 a         1780 c         1624 g         1009 t
ORIGIN      
        1 gcactaaatt ttcaattcgc aatcggacag ggtcccggaa aattccgcgg tgcgatcgtc
       61 gagaacattt cgattctgaa tgacggtgta actcaaaatc ttggacatgg tccggtcgcc
      121 ctcggttttt tcaacaccgg cattaatgtc accggctgca actatccgta cttcgacgac
      181 gtcacgatca acggctgcga aggcgcaggc gccgtcgtcg accgctccga cggcgatccc
      241 gcctatgcga tgcaaactgc gctggtcgtc gacgactgct acgaccccaa gctccaggac
      301 tgccggttct ggcatggaca ggtgggcgtc agctatcaga acaatgcccg tggcgaggat
      361 cccgccgccc acgtccaggc gttccagatg cttggctgca agctgaacgg tttccagacc
      421 ggcctcaagt tttcgaataa cggcgtcgtt tttccccaca ccgtcattac gggcaacgaa
      481 ggcacctatc gcgacgtcgg cttcgacatc ggcgatccgt tcaagatcat gctgaccgac
      541 aaccagttca gcagcgacgg cgacggcacc tacacgagaa acggcggcgt gtactacgac
      601 atgtggttca aacacgccaa cgtggtcacc atcgtcggca gcgggttcaa caccaaaagc
      661 gatcccgaca gggtctgcat ctacatcgac gccgtgacgg acaccggcgg cgaaaacacc
      721 catggcggca aacattttct cgtggcggag aaccagttcg gagatccggt gggcggctcg
      781 cctttcggaa ccaatgccgt caaggtgacc gccggcgcaa cggatatcca gatcgggcat
      841 aatgattacg cgggaacctt ctccgacctt ttggtgaacg acgcctccgg aaatgccgtc
      901 atctggaacg cgagcgtcag ccaggcgcag acgctcgtag cggaggtgca gcccgttcgt
      961 tccggttcca ctcccacgaa cgccggcccc atcatcgatc tataccagaa cgtgcacgcg
     1021 ggcggcggag ccaacgacac gattggcgca ttgcagatgt cggctaacct ggagtttggc
     1081 gagaccaaag cgacgtttgc cgccattacg gcgagcattc agtcggcagt cgccgaagcg
     1141 cttatgggaa tcctgaacat ctccattgtc gccggcgctt acctgcagaa cgtgttgacg
     1201 ctttcgctgt cgaacatcaa catgggcacg acggggacga cgtcggtcac gccggccgtc
     1261 gacaatgcga tcacgctggg cagcgccgcg cagagatggt cgaagaccta tgccgtcgcc
     1321 cgctacttct ccggcggcgt gttcgactcc accggttcgg gttcgccgga tggcgtggtg
     1381 acggcgccgg tgggttcgac ctaccgcaac acgggcggcg ggagcatgcc caccttctgg
     1441 gtgaagcaga cgggcaccgg caacacgggc tgggtcggga agtaagctgc cgcgcctcgc
     1501 ggagccggcg cgggcgtctg cgtctacggt ggaatggaat ggtacagctg ggtgggttcg
     1561 aaccgccgac ctccggatcc acaatccggc gctctaacca actgagctac agctgcccgc
     1621 gaggggcggg aacctaggat tcctgcctct tgattgcaag gttgccacga accctcatgg
     1681 tgagcctgtc gaaccatcct tcgacaagct caggatgagg aggataccct caccctgagc
     1741 ctgtcgacgc gccccttgcg gaaaaccgcg cccgcgagga gaagatatgc ggagcgcgaa
     1801 gcgctcacac atccaacctc cccacaaggg ggaggtaagt cgaagcaacg ttgcgacatg
     1861 gcgaatctcg acatcgatcc cgggcgggcg acactgtcgc tgtggcggcg cgtgctcgaa
     1921 accgcggcgc ccatctctct cgcgcccgcc gcctggccga tcatcgagcg cgcgcgccac
     1981 acggtcgaag acgcgctcgc cgccggccgc gtgctctatg gcgtcaacac cggcttcggc
     2041 aagctcgccc agatgcgcat tgcgccgggc gagatcaaga ctttgcagcg caatctggtg
     2101 ctttcccatt ccgcgggcgt gggcgagctt ctgccggacg cactcgtccg cctcgtgatg
     2161 gcgctgaaag tggcgagcct cgggcgcggg ttttcgggcg tgcgccggat tctcgtcgag
     2221 cggctgctgc agcttctgaa cgccaatgtt ctgccggaga ttccctcgca gggctcggtc
     2281 ggcgcctcgg gcgatctcgc tcccctcgcc catctcgccg ctgtcctgat cggcgaaggg
     2341 cgcgcgcgca tcgccggccg ttccatgccc gcgggagatg cactggcgca agccggcatt
     2401 gcaccgatcg aactcgaagc aaaggaaggc ctcgcgttgc tcaacggcac gcaggtttcg
     2461 acggcgcttg cgctctcggc gctgttcgcg gcggaagcca atctcgaagc ggcccttgtc
     2521 gcgggcgcgc tttcgaccga tgcggtgaag ggcagcgaca cgccgttcga tgcgcgcatt
     2581 catgacgcgc gcggccagcc cggccagatc gaagcggcgc atgcgctgcg caccctgatg
     2641 gcgggaagcg aaatccgcgc cagccaccgg gattgccccc gcgtgcagga tccctattcg
     2701 atccgctgcc agccccaggt gctgggtccc tgtctcgaca ttctgcgcaa cgtggccggc
     2761 gtgcttgcca tcgaagccaa tgcggcgacc gacaatccgc tggtgttcga gaatggcgac
     2821 acgctgtccg gcggaaattt ccacgccgag cccgtggcgc tcgccgccga cacgctggcg
     2881 cttgcgatcg ccgagacggg ggcgctgtcg gagcggcgca tcgcgctgct gatcgattcc
     2941 gcgttgagcg gattgccggc cttcctcgcg catgatggtg gcgttaattc cggtttcatg
     3001 atcgcccatg tcaccgcggc ctcgctcgcc tcggagaaca aatccctcgc ccaccccgcg
     3061 tccgtcgatt cgctgcccac ttccgccaac caggaggact tcgtctcgat ggcgaccttc
     3121 gccgcgcgcc ggctgggcgc gatgaacgcc aacaccgcca acatcctcgc cgtcgagctg
     3181 ctcgccgccg ccgaaggaat cgaattccac cgccccctca aatcctcggc ggcgctcgaa
     3241 acagcgcacg cagccctgcg cgcccgggtc ccgcgcttcg accgcgaccg gatgatgtcg
     3301 ccggcgattg ccgcagcgtc ggaacttgtg tcgggtcgcg cgttcgaaaa acttctcccg
     3361 gatgatttcc gcttgccgtc gcagagatga attgcggttg ccattcccct ttttcgctgt
     3421 catggcccgg caccccggtc cgcgcgagag cgcggccgga gtgtaaactc cgagcgggcc
     3481 gcccagatga aacccgcaca gcgttgctcg tcatagggac gttcatttcg aaacggtccg
     3541 tcagtaccat ccgggtggcc cgcttgcgcg ggccatgaca aacaaggggg tgcggcaccg
     3601 ttcgtgctac tcactggcgg agaatccact ccggagaact tcccatgacc tggccgatga
     3661 tcgcgcccat cctgatgttg agcgtgtcga acatcttcat gaccttcgcc tggtacgggc
     3721 agttcaaatt ccccgccgcg tcgctgtgga tcgtgattcc cgtctcctgg ggaatagcat
     3781 tcttcgaata ttgctttgcc gtccccgcca atcgcctcgg gcacctcgtc tactcggcgg
     3841 cgcagctcaa gaccatgcag gagatcatca cgctgcttgt gttcggcgtg ttcgccgtca
     3901 cctatctcgg cgatacgatc cgctggaacc acttcgccgc tttcgcctgc ctggtggctg
     3961 cggcgttctt ctcgttcaac aagtggtcgt gaatcgcacc cgccgagttt gatcgagctg
     4021 gcgcactgtt agcccacgtg ttccccctca cccgcgcttg cacggatttc acggcacgta
     4081 tctgcgtccc tctgccacaa ggggagaggg gaatccgcgc gctgcatcaa cggcgggaaa
     4141 tcggcaggac acttccctct ccccttgccc gcctatcccc cggactgtga tccgggggtt
     4201 cggcgggtct ggtgactctc tctgtccgcc gtagccttgg cgtaggcgga tgggagaggg
     4261 ctcaagcacg ctgtcccttc aaccgctcca gcgcgggtga ggggtagcga aacgttctgt
     4321 gtacggaaaa taatttgcgg cgcgggcatt tttccgcttg acgggtttga tcaaacgcac
     4381 tactcatttc ggcatgttga gtttccgcgc ccggcgctcc tgatcgagct gccgggtttt
     4441 tctttcctct agctccgttt tgtcatggcc cgccacgcgg tcggcgcgag agttatgcgg
     4501 aacgcgaatt gaagcgcagg ggattgcagg cgatgcccga tttgacgatt cccggcgcgc
     4561 gtgtgagcgc gattccccca tcgcttgtca cgcgcgtcgg ccaggcggtg cgttacgcca
     4621 taaccggcgt aacgcccgcc acctggttcg ggccgatgca gccgctcaaa cccttcgcgc
     4681 cggagggcac gggcggccgc gctttcgact atcccgtcgg ctacaatctt cgctatcagc
     4741 cgcgcgccga cgagcccatt tccttcgtcg atctccgggc gcttgccgat gggtgcgatc
     4801 tgcttcgcct cgtcatcgag actcgcaaag accagatcga aggccagacc tgggacgtgc
     4861 gcccgagaaa attgcccaac ggcgcccatc ccgccgcgag cgccttcgcc accgagatcg
     4921 cgaaagccaa ggccttcttc gcctacccgg accgggagct cgatctcgcc tcgtggatcc
     4981 gtgcgcttgc cgaagacatg ttcgtcaccg acgccgtgtc gatctacctg cggcctgacc
     5041 gcagacaggg aatctacgga attgaagtga tcgacggcgc gacgattgcg ccgcgcatcg
     5101 acgcctgggg ccgcacgccg caggcgccgg atgtcgccta tcagcaaatc cttcatggtg
     5161 tgccggcggc ggatttttcg cgcgaccagc tcgtctattt cccgcgcaac cgaaggccgg
     5221 gacatgtcta cggctattcg cccgtcgaac aggtgatcgt cacggccaac acgctcatcc
     5281 gccgcagcct gcatcagctt gcctactaca ccgaaggtaa tctggtggac ggacttttca
     5341 cctcgccgcc ggaatgga
//
LOCUS       DASZMC010000339         4938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3446320,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000339 DASZMC010000000
VERSION     DASZMC010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3446320"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1409
                     /locus_tag="VG274_06935"
     CDS             156..1409
                     /locus_tag="VG274_06935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWE06424.1"
                     /translation="MTNPTFHSLGVAEPIQRALAAENYLEPTPIQARAIPLILSGRDV
                     VGIAQTGTGKTAAFGLPVLQRLAADRTKAGPHAVRALILAPTRELALQIHDSLGRYGR
                     HLGLRLAVVLGGVNQSRQVEAARRGVDILVATPGRLLDLVNQKHIRLGAATTLVIDEA
                     DRMFDMGFIRDVRRIVAQLPRERQSMLFSATMPADVAHLVAEVLRNPARIDISPTAVT
                     VERIEQRVHFVAEKDKRALLLDLLRDHAMTRVIVFTRTKHHANRVTELLGKAGHRVNA
                     IHGNKSQNARQHALEGFRAGRSRILVATDIASRGIDIDDVSHIVNLDIPDVAENYVHR
                     IGRTARAGSGGTAISFCDPSERESLRAVERLVKRPLAVAGGERRPPVFAEIAEPKAER
                     NSPRSAQRPRSRKRFRNRRNSPKAA"
     gene            1435..1653
                     /locus_tag="VG274_06940"
     CDS             1435..1653
                     /locus_tag="VG274_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06425.1"
                     /translation="MKKKKYELPRPYSAPTKDTRFAGTFEVLVPAPGRSKPHRVPLQF
                     ESQENAERWIHSADGKEAIASLLANSGS"
     gene            1863..2435
                     /locus_tag="VG274_06945"
     CDS             1863..2435
                     /locus_tag="VG274_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06426.1"
                     /translation="MLEDDLRSRLMPGERILWSGQPCRGLLLTGRDIFLVPFSLIWCG
                     GAIVWEAAVLGLLNRAGAHSHAPFFFALWGVPFVLLGLYFVFGRFITDAWIRRGTIYG
                     VTDRRVLILRNGIFNRFVALGVDRLPEIALSEKRSGRGTIIFGPGLSLRMQPGVSGFL
                     PSLEATPRFLEIENARSVFDQVQRLAAPGK"
     gene            2438..3445
                     /locus_tag="VG274_06950"
     CDS             2438..3445
                     /locus_tag="VG274_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWE06427.1"
                     /translation="MDISSSAIVTERKLSAIAGRIVSGANAVTNLRRLSGGASQELWR
                     FEVANGNRSSCAILRRAPGGERVSTASVGPEMEARLMSAAGARGAPVPPVLYVLAPED
                     GLGRGFVSGFVEGETLGGRIVRTEALASARAVLARQCGQILARIHALDANEFPGLRRF
                     TPLELLDDWQAAYRATETPRPVFELAFRWLRANCPKSPEHPRVVHGDFRNGNLIVGPD
                     GVRAVLDWELAHVGDPMEDLGWLCVNSWRFGAMEKIVGGFGELADLFGGYEAEGGAAD
                     FASIRWWEIFGTLRWGVMCAGMPRAFREFDRTVERAVIARRASETEIDLMRLLTAHKA
                     H"
     gene            3448..3834
                     /locus_tag="VG274_06955"
     CDS             3448..3834
                     /locus_tag="VG274_06955"
                     /inference="COORDINATES: protein motif:HMM:NF040046.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HWE06428.1"
                     /translation="MQDHPLPHEIVAAVAEFLRAESHSGFQLRVAGNALDIARRQLEL
                     SPAADAAELARLTELLEHGGALADLNRELAQAIASGAMDFSTPGLAAHLWETTLAKLS
                     VDQPTYSGYSAALRTLRQTHPSTSSG"
     gene            4056..>4938
                     /locus_tag="VG274_06960"
     CDS             4056..>4938
                     /locus_tag="VG274_06960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWE06429.1"
                     /translation="MDFSLPQELVDYLAELDRFIDTTIKAIERRDDNIRFFDHRREHA
                     RTDWDNHGLPRPEWEAVLAEAREAAEKAGHYRYAWPVEMGGKGGTNLAMAVIREHLAA
                     RGLGLHNDLQNEHSIVGNNPFVLMFRNFATEAQFAMYGAKLRNGEIRTAFGLTEPKHG
                     SDATFMETRAVRETRNNAAGWRIDGEKMWTTGMHAVTHVMVFARTSGEDGEARGITVF
                     LVPANHPGVKIEEWLWTFNMPTDHPRVSFTDVWLPDDAMWGIEGRGLGLGQSFVHQNR
                     IRQAASSLGATVFCVNES"
BASE COUNT          871 a         1617 c         1598 g          852 t
ORIGIN      
        1 gggcgcatcc tcgcggtcgc accgctcgtg ccgattccat ggcgccagtc accgcgaagg
       61 caagattttc aactcaccgt tcgctccaaa tgtgcgtgga agacggcaaa ttgccgtaag
      121 cgccttccgg cgctcccgtg catgaaggaa caattgtgac gaatccgaca tttcattcgc
      181 tcggcgtggc cgagcccatc cagcgtgcgc tggcggccga aaactatctc gagccgacgc
      241 cgatccaggc gcgcgccatt cccctcattc tctccggccg cgacgtcgtg ggcatcgccc
      301 agaccggcac cgggaagacc gcggccttcg ggctgccggt gctgcagcgt ctcgccgcgg
      361 accgcacgaa ggcagggcct cacgcagtgc gcgcgctgat cctggcgccg acgcgcgagc
      421 tcgcgctgca aatccatgac agcctcggcc gctatggccg gcatctcggc ctgcgcctcg
      481 ccgtcgttct gggcggcgtc aaccagagcc gccaggtcga ggcggcgcgc cgcggcgtcg
      541 acatcctggt ggcaacgccg ggacggctgc tcgatctcgt caaccagaaa cacatccggc
      601 tgggcgcggc gacgacgctc gtcatcgacg aggccgaccg gatgttcgac atgggcttca
      661 tccgcgacgt tcgccggatc gtcgcccagc tgccgcgcga gcgccagtcg atgctgttct
      721 ccgccaccat gccggccgac gtcgcgcatc tggtggcgga agtgctgcgc aaccccgcgc
      781 gcatcgacat ttcgcccacg gcggtgacgg tggagcggat cgaacagcgc gtgcatttcg
      841 tggccgaaaa agacaagcgc gccctgctgc tcgatcttct gcgcgaccac gcgatgacgc
      901 gggtgatcgt gttcacgcgc accaaacatc acgcgaaccg ggtgaccgag ctgctcggca
      961 aggcgggcca ccgcgtcaat gcgattcacg gcaacaagtc gcagaacgcg cgccagcatg
     1021 ccctcgaagg atttcgcgcc ggccgatcgc gcatcctggt cgccaccgat atcgcttcgc
     1081 gcggcatcga catcgacgac gtttcgcaca tcgtcaatct ggacattccc gacgtggccg
     1141 aaaactacgt gcaccgcatc ggccgcaccg cgcgcgccgg cagcggcggc acggcgatat
     1201 ccttctgcga tccgtccgag cgcgagtcgc tccgcgccgt cgagcggctg gtgaagcggc
     1261 cgcttgcggt tgcgggcggc gaacgccggc cgccggtgtt tgccgagatc gccgagccga
     1321 aagccgaacg caattctccg cgctccgccc aacgtccgcg cagccgcaaa cggttccgca
     1381 accggagaaa ttcccccaaa gccgcataga gttggaagac gaggatccac gccgatgaaa
     1441 aagaagaaat acgagctgcc gcgcccctac tccgcgccga ccaaagacac gcgctttgcg
     1501 ggcactttcg aagtgctcgt gccggcgccg ggacgctcga agccgcatcg cgttcccctg
     1561 cagttcgaat ctcaggaaaa cgccgaacgc tggatccaca gcgcggacgg caaggaagcc
     1621 atcgcaagcc tgctggcgaa cagcggaagc taactcggaa ttaagtgata cgcatgcaag
     1681 atttagcgca aaggacgcag aggctgcgca gagggcgcag aaaaaaaagc cggggcggtg
     1741 cgcgcgccta tgttttcccc cgcgtccact gcgcaacctc tgcgttctcc gcgccaaaat
     1801 cttgttggtt cacgaatcac ttgcgttgaa gagtaggcac gaagagcgct gccggagggg
     1861 atgtgctgga ggacgacctc agaagccggt tgatgcccgg cgagagaatt ctctggtccg
     1921 ggcaaccgtg ccggggcttg ctcctcaccg gccgggatat tttcctcgtg ccgttcagcc
     1981 tgatctggtg cggcggcgcc attgtctggg aagcggctgt tcttggcctg ctgaaccgcg
     2041 caggcgcgca ttcgcacgcg ccgttctttt ttgcgctgtg gggcgtgcct ttcgttctgc
     2101 tcgggctcta ctttgtgttc ggccgcttca tcaccgacgc gtggatacgg cgcggaacca
     2161 tctatggcgt aaccgacagg cgcgtgctga tcctgcggaa cggcattttc aacaggttcg
     2221 tcgcgctcgg cgtcgaccgg ctgcccgaga ttgccctttc ggaaaagaga agcggccgtg
     2281 gcacaataat tttcggaccc ggtctctccc tgcggatgca gccgggagtc tcgggctttt
     2341 tgccatcgct cgaagcaacc ccacgatttc tcgagatcga gaacgcgaga agcgtgttcg
     2401 accaagtaca gcgattggcc gcgcccggga aatgatcgtg gacatatcct cgtccgccat
     2461 cgtaaccgaa cggaagctga gcgcgattgc gggccgaatt gtatccggag caaacgcggt
     2521 cacgaatctc cgccgattgt cgggcggcgc gagccaggag ctttggcgct tcgaggtcgc
     2581 gaatggcaat cgctcgtctt gtgcgatctt gcgccgcgcg ccgggcggcg agcgcgtctc
     2641 gacagcttct gtcgggccgg agatggaagc gcgattgatg tcggcagcgg gagcgcgcgg
     2701 cgcgcctgtg ccgccggtgc tttatgtgct tgcgcccgag gacggtctgg gccgcggttt
     2761 cgtctcgggc ttcgtcgaag gcgaaacgtt gggcgggcgc atcgtcagga ccgaagccct
     2821 ggcgtccgcg cgcgcggtgc tggcgcgcca gtgcggacag atcctcgcac gcattcacgc
     2881 gcttgatgcc aacgaatttc ctggcctgcg ccgcttcacg cctctggagc tgctcgacga
     2941 ctggcaggcg gcctatcgcg cgacggaaac gccgcggcct gtgttcgagc tggcgttccg
     3001 ctggctgaga gcgaactgtc ccaaatcacc cgaacacccg cgcgtcgtgc atggggattt
     3061 ccgcaacggc aatctgatcg tggggccgga cggcgtgcgg gcggtgctgg actgggagct
     3121 cgcccatgtg ggcgacccga tggaggatct cggctggctc tgcgtcaatt cctggcgctt
     3181 cggcgcgatg gagaagatcg tcggcggctt cggcgagctc gcggatctat tcgggggcta
     3241 tgaagcggaa ggcggcgcgg cggattttgc gtcgatccgg tggtgggaaa tcttcggcac
     3301 gctgcgctgg ggcgtgatgt gcgcaggcat gccgcgcgcc tttcgggaat tcgatcggac
     3361 cgtcgaacgc gcagtgatcg cgcggcgcgc ttcggaaacg gagatcgatc tgatgcgatt
     3421 gctgaccgcg cacaaggcgc attgaccatg caggaccatc cgcttccgca cgagatcgtc
     3481 gccgcggtgg cggagtttct tcgcgcggaa tcccattccg gcttccagct gcgcgtcgcc
     3541 ggcaacgcgc tcgacatcgc acgccgccag ctcgaacttt cgcccgccgc cgacgccgcg
     3601 gagctggcgc gattgaccga gctgctcgaa catggcggcg cgcttgccga tttgaaccgg
     3661 gaactggcgc aggcgattgc gagcggcgcg atggatttct ccacgcccgg actcgccgcg
     3721 catctgtggg aaacgacgct cgcgaaactc agcgtcgacc agcccaccta ttccggctac
     3781 agcgccgcac tgcgcaccct tcgacagact cacccttcga caagctcagg gtgagccgta
     3841 agaaagactc accgccaagt cgccaagagc gccaaggcgc ccgtggcgat ctttgagatt
     3901 cacagggagc cagtgagggc agtgagattt gttgcgcgcc ttcggcgcgc cattttccct
     3961 cgctgccctc actggctccc tgtgaaaatt cttcttggcg gcttggcgtc ttggcggttc
     4021 actctctctc ccgacgcgct caccacaggc tctcaatgga cttctcgctg ccgcaggagc
     4081 ttgtcgacta tctcgccgag cttgaccgct tcatcgacac cacgatcaaa gcgatcgagc
     4141 ggcgcgacga caatatccgc ttcttcgatc atcggcgcga acatgcgcgc accgactggg
     4201 acaatcacgg cctgccgcgc ccggaatggg aggcggtgct cgccgaggcg cgagaggcgg
     4261 ccgaaaaggc tggccattac cgctacgcct ggccggtgga gatgggcggc aagggcggca
     4321 cgaatctcgc catggcggtg atccgcgagc atctcgcggc caggggcctc ggcctgcaca
     4381 acgatctgca gaacgaacat tcgatcgtcg gcaacaatcc cttcgtgctg atgttcagga
     4441 atttcgccac cgaagcgcaa ttcgcgatgt atggcgcgaa gctgcgcaac ggcgagatcc
     4501 gcaccgcgtt cggcctgacc gaacccaaac acggctcgga cgcgaccttc atggaaacgc
     4561 gcgcggtgcg cgagacacgg aacaacgccg ccgggtggcg catcgatggc gagaagatgt
     4621 ggacgacggg catgcacgcc gtgacccacg tcatggtctt cgcgcgcacc agcggcgagg
     4681 acggcgaggc gcgcggcatc accgtcttcc tcgtgcccgc caatcatccc ggcgtgaaga
     4741 tcgaggaatg gctgtggacg ttcaacatgc cgaccgacca tccgcgcgtc agcttcaccg
     4801 atgtgtggct acccgacgac gccatgtggg gcatcgaggg ccgcgggctc gggctcggcc
     4861 agagcttcgt gcaccagaac cgcatccgcc aggccgcgtc atccctgggt gcgacggtct
     4921 tctgcgtcaa cgaaagcg
//
LOCUS       DASZMC010000340         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4815314,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000340 DASZMC010000000
VERSION     DASZMC010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4815314"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VG274_06965"
     CDS             complement(<1..180)
                     /locus_tag="VG274_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06430.1"
                     /translation="MALDYFEQMGGERVRDPQRVVFALDHYAPPPSRQTALLHQRMRA
                     FASQHDIEVWDVGEGI"
     gene            complement(316..1752)
                     /locus_tag="VG274_06970"
     CDS             complement(316..1752)
                     /locus_tag="VG274_06970"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWE06431.1"
                     /translation="MQTTVERKPLEQIEADALIVPVIEGAKEKRFGAADLCESGEVAG
                     KPLELTLIHYPHGVAAKRVVLAGGGKSEKFDAAEMRRLVSAAVRMLKPKGVKRVAVVL
                     EGSFAGADFTAAAVEGAILGDFEPDRYKGGEKKALESVAIVGGDEKAVERGRILAEAQ
                     NFARNLANEPANLLTPMKMAEAARAMCEESGIKGVLECEVLEQDQMKKLGMGALLGVA
                     QGSAEPPALIVIRYKPASAKSKDHLALVGKGVTFDTGGISIKPSDGMEKMKYDMCGGA
                     DMMAAMRAIAQLKPTVTVSAFIPCVENMPGSRAQRPGDIVTAMSGKTIEVLNTDAEGR
                     LILADAMAYARKQGVTHMVDAATLTGAIVVALAHIHVGLFSNNDDMRDRVLNAAKAEG
                     ERMWAMPLDDDYKDYLKSAFADLGNIGGRWGGAITAAAFLKEFAEDTPWVHLDIAGTA
                     WLDDAKPYMAKGPTGVAVRTLVRLALDW"
     gene            complement(1816..>2416)
                     /locus_tag="VG274_06975"
     CDS             complement(1816..>2416)
                     /locus_tag="VG274_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWE06432.1"
                     /translation="GLRLLLESVTDFQVVADAATGRDAVQLAEQHRPDVVVMDVAMPV
                     LNGIEAARQITSKQPSISIVFLSMHGDESYVLRALKAGAKAYLLKDSAETDLIQAVKA
                     VNEGKAFFSPAISKLLVEDYVRQMQERGVEDTFDLLTTREREVLQLLAQGKSNKEAAA
                     LLEMSPHTVETHRGKILEKLNLHGQAELILYAVRKRLIR"
BASE COUNT          402 a          850 c          649 g          515 t
ORIGIN      
        1 gatgccttca ccgacgtccc acacttcaat gtcgtgctgc gatgcgaacg cacgcatgcg
       61 ttgatgcagc agcgccgtct ggcggctggg cggcggcgca tagtgatcga gcgcgaacac
      121 gacacgctgc gggtcgcgca cgcgctcgcc gcccatctgc tcgaagtagt cgagcgccat
      181 gggcgtcgat ccatccgtac ccaaagcgca atccacgcgg ctgatgacaa cgtcgcctgc
      241 gcgcgcgtcg cgccccgatt ttgccgagag gatcttctcg ctaattgttt taccggccat
      301 gtcgcgccag ctgctttacc aatcgagggc cagccgcacc agcgtgcgaa cggcgacgcc
      361 cgtcggtccc ttggccatat acggcttcgc gtcgtccagc cacgcggtgc cggcgatatc
      421 caagtgtacc cagggcgtgt cctcggcaaa ctctttcaga aacgcggcgg ctgtgatcgc
      481 accgccccag cgcccgccga tgtttcccag gtccgcaaat gcgctcttca aataatcttt
      541 gtaatcgtcg tcgagcggca tcgcccacat gcgctcgcct tccgctttcg cggcgttcag
      601 aacccggtcg cgcatgtcat cattgttcga aaatagaccg acgtgaatgt gcgccagcgc
      661 gaccacaatt gcgccggtta acgtggcggc gtccaccata tgcgtcacgc cctgtttgcg
      721 cgcatatgcc atcgcgtccg ccagaatcaa gcggccttct gcgtcggtgt tcaacacctc
      781 gatggttttg cccgacattg ccgtgacgat gtcgcctggc cgttgcgcgc ggctgcctgg
      841 catgttttcg acgcacggga tgaacgcgct cactgtcacc gtgggcttca gttgcgcgat
      901 cgcgcgcatc gccgccatca tatcggcgcc gccgcacatg tcgtacttca tcttttccat
      961 gccgtcggac ggtttaatcg agatgccgcc ggtgtcgaag gtcaccccct taccgaccag
     1021 cgccaggtga tccttcgatt tcgccgaagc gggcttatag cggatcacga tcagcgccgg
     1081 cggctccgcg ctgccctgcg ccacacccag cagtgcgccc attcccagct ttttcatctg
     1141 gtcctgttcc agcacctcgc actccagcac gcctttgatg ccgctctctt cgcacatcgc
     1201 gcgtgcggcc tcggccattt tcatcggcgt cagcagattg gcgggctcat tcgccagatt
     1261 gcgcgcaaaa ttctgcgcct ctgcgagaat tcgtccacgc tccacggcct tttcatcgcc
     1321 gcccacgatt gcgaccgact ccaacgcctt cttctcgccg cccttgtagc ggtcgggctc
     1381 aaaatcgccc aagattgcgc cttccaccgc cgccgccgtg aaatccgctc ccgcgaagga
     1441 accttcgaga accactgcga cccgcttcac gcctttcggc ttcaacatac gcaccgccgc
     1501 gctcaccagc ctgcgcattt cggcggcatc gaatttctcc gatttgccac cgccggccag
     1561 taccacgcgc ttcgcggcca cgccgtgtgg ataatggatc agcgtcagtt ccagcggctt
     1621 gcccgccacc tcacccgatt cgcaaaggtc ggcggcgccg aagcgtttct cctttgcgcc
     1681 ctctataacc ggcacgatca gcgcatcggc ctcaatctgt tccagcggtt tgcgttcgac
     1741 agtagtttgc attagttcga ttgtcccgaa ccgcgcggat tcccgcaatc cggcgggcgc
     1801 ttgcggcgca gcgtgtcagc gaatcaaccg cttgcgcacc gcgtagagga tcagttcggc
     1861 ctgtccgtgc agattcagct tctccagaat cttgccgcga tgcgtttcca ccgtgtgcgg
     1921 gctcatttcc agcagtgcgg ccgcctcctt gttgctcttg ccctgcgcca gcagttgcag
     1981 cacttcacgt tcgcgcgtcg tcagcaggtc gaaggtgtct tccacgccgc gctcctgcat
     2041 ctggcggacg tagtcttcca ccaaaagctt gctgatggcc gggctgaaga acgctttgcc
     2101 ttcattgact gcctttaccg cctgaatcag gtccgtttcg gccgaatcct tcagcaaata
     2161 ggcctttgcg cctgccttca aggcgcgcag cacataactt tcatcgccat gcatgctcag
     2221 gaagacgatc gaaatggacg gctgcttgga tgtgatctgc cgcgccgcct cgattccgtt
     2281 caacacgggc atcgccacgt ccatcaccac cacgtccggg cgatgctgtt ccgccagttg
     2341 cactgcatcg cggcccgtag ccgcgtccgc caccacttga aaatccgtca cgctctccag
     2401 cagcagccgc aggcct
//
LOCUS       DASZMC010000341         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_717579,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000341 DASZMC010000000
VERSION     DASZMC010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717579"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..369)
                     /locus_tag="VG274_06980"
     CDS             complement(70..369)
                     /locus_tag="VG274_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06433.1"
                     /translation="MFKLIGVAAVLAVLLAGAAYTKPSRDSLHEAATAYADNSSVVGS
                     VATRVTGLFGNDAYDDYIFFDRYRVYVGRNPKVDCFGVFGKTSCSMPKIEDTGEG"
     gene            474..1631
                     /locus_tag="VG274_06985"
     CDS             474..1631
                     /locus_tag="VG274_06985"
                     /inference="COORDINATES: protein motif:HMM:NF002328.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWE06434.1"
                     /translation="MRAPFAVRPAESRGRFYGEPESATRTCYQRDRDRIIHSTAFRRL
                     KHKTQVFVQHEGDYYRTRLTHSLEVAQIARSLARVLALDEDLAETVALAHDLGHTPFG
                     HAGEEALSAATASIGGFDHNAHALRLVTKLEHRYADFDGLNLTWETLEGLVKHNGPLK
                     APLPMPIASFDARWPLELKTWPGLEAQIAALSDDIAYVNHDIDDGLRAELFTIADLSS
                     APLAGAQTRAVEQRYPDLEPGRFIGELVRTLMSTLIDDLLTETKRRIAAADPKSAADV
                     RAQDHAIAALSPGMESEVKALKGFLFERMYRHPRVMATMNRAKDVVTELFEALDRDPS
                     LLPADWAARCGAPGDPITGGVVRDYIAGMTDNFALSEYQRVFHREIALATP"
     gene            1683..2582
                     /locus_tag="VG274_06990"
     CDS             1683..2582
                     /locus_tag="VG274_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWE06435.1"
                     /translation="MPSARGTYEPPDDIHVFDGSEDDDDVEGSRLPLLIVIALFVLAA
                     FGGVVWMAYTQGVARGHSEAPRTIVAQSGPPKVAANGEGGTTPYKGLKIYEQAAPADE
                     AADHQSAPPPTNPDVNAQAAAEPETSVVTMPPAQTPAETTPNSAASKEPPLSVAKSLT
                     APAPKVVAPPVPKATPPVVKTVKATPPSEQATSAPAQLGTKIVAAEPPPVEHVAPATG
                     GAYVLQIGAYKSEAEADAAWKTYSSKHAGTLSGRAKDVKQVDLGEKGTWYRLRVGSFA
                     DKAAAAALCSKLTAEGGACFPAK"
     gene            2612..>2989
                     /locus_tag="VG274_06995"
     CDS             2612..>2989
                     /locus_tag="VG274_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HWE06436.1"
                     /translation="MRTRAIYGCAGTTLASEEQAFFRETQPWGFIVFARNIVDPAQLR
                     TLAESLRETVNDPRAPILIDQEGGRVARLKPPHWQERLAAARFGELYLRDPEVAREAV
                     YLNARLLAHDLCEAGINVDCLPLL"
BASE COUNT          590 a          940 c          937 g          522 t
ORIGIN      
        1 aggatcgtcg cgatgcgctt tgcggcgcgc gcggagaccg tgacgggcat tgcggtttca
       61 gtgtccattc tagccttcac ctgtgtcctc gatcttcggc atcgaacagg acgtcttccc
      121 gaacacgccg aaacagtcga ccttcgggtt cctgccaaca tacacccggt aacggtcgaa
      181 gaatatgtag tcatcgtagg cgtcgtttcc aaataacccc gtcacccgtg tggccaccga
      241 tccgaccacg ctcgaattat ccgcataggc cgtcgccgct tcatgcagtg agtcccggct
      301 gggcttggta taggcggcgc cagccagcaa aaccgccaga accgccgcga ctccgattag
      361 cttgaacata tgttccctcc cgctttgggt tgtacgttat ggttaccgac atgcggcaag
      421 attttgatca actccccggc aggctcatcg gcccgctata cgagccgcct tccatgcggg
      481 cgccattcgc cgtgcggcca gcggaaagcc gggggaggtt ctatggcgag ccggaaagcg
      541 cgacacgcac ctgctaccag cgcgaccgcg accggatcat ccactccacg gcgttccgca
      601 ggctcaagca caagacacaa gtcttcgtgc agcatgaagg cgactactac cgcacgcggc
      661 tgacgcattc gctggaggtc gcgcagatcg cgcggtcgct ggcgcgcgtt ctggcgctcg
      721 acgaagacct cgccgaaacg gtcgcactcg cacacgatct tggtcacaca ccattcggac
      781 atgctggcga agaggcgttg agcgcggcga cggcctccat cggcggcttc gatcacaatg
      841 cgcatgcgct ccgcctcgtg accaagctgg aacatcgcta cgccgatttc gacgggttga
      901 acctgacgtg ggaaacgctg gagggattgg tcaagcacaa tgggccgttg aaggcgcctc
      961 tgcccatgcc gatcgcgagt ttcgatgcgc gctggccgct tgaactcaaa acctggccgg
     1021 gcctcgaagc gcagattgcg gcgttgtcgg acgacatcgc ctatgtgaac cacgacattg
     1081 acgatggcct gcgcgcggaa ctgttcacga tcgcggacct gtcgtcggcg ccactggccg
     1141 gtgcgcaaac gagggcggtg gaacagcgtt accccgatct cgaacccggc cgcttcatcg
     1201 gggaacttgt ccgtacactg atgagcacgc tgatcgacga cctgctgacc gaaacgaagc
     1261 gccggatcgc cgccgccgat ccgaagtctg ctgcggatgt acgcgctcag gaccacgcca
     1321 tcgccgcgct ctcgccgggc atggaaagcg aagtgaaggc gctcaaagga ttccttttcg
     1381 agcgcatgta tcgccatccg cgcgtcatgg cgacgatgaa ccgggccaag gacgtcgtga
     1441 cggaactgtt cgaggcgctg gaccgcgacc cgtccctgtt gcctgcggat tgggcagcgc
     1501 gctgcggggc gccgggcgat ccgatcaccg gcggcgttgt gcgcgactat atcgccggta
     1561 tgaccgacaa cttcgcactt tccgaatatc agcgggtatt ccacagggaa attgccttgg
     1621 ccacgccatg atttgcctgc acgagatgct aaattccctt ttgagattcg cggagccgag
     1681 ccatgcccag cgcacgtggt acctatgagc cgcccgacga cattcatgtc ttcgacgggt
     1741 cggaagacga tgacgacgtc gagggctcgc gccttccgct gctgatcgtc atcgcgctgt
     1801 tcgtcctggc ggcgttcggc ggcgtggtgt ggatggccta tacgcagggc gtggcacgcg
     1861 gacattccga agcgccgcgg accatcgtgg cgcaatccgg tccgccaaaa gtagccgcaa
     1921 acggcgaggg cggcacgacg ccttacaaag ggctcaagat ttacgagcag gctgcacctg
     1981 ctgacgaagc cgccgatcat cagagcgcac cgccgccgac aaatcccgat gtgaacgcgc
     2041 aagcagccgc tgaaccggag acctccgtcg tcaccatgcc gccagcgcaa acgcctgccg
     2101 aaacaacgcc aaactccgcc gccagcaagg aaccgccctt gtcggtcgcg aaatcgctga
     2161 ccgcacctgc accgaaggtg gttgctccac cggtaccgaa agcaacgccg cctgtggtga
     2221 agaccgtgaa ggcaacgccg ccgtctgaac aggccacatc tgcgccagcg caactcggga
     2281 caaagatagt cgccgctgag ccgccgcccg ttgagcatgt ggcaccggcc acgggtggtg
     2341 cgtatgtcct gcagatcggg gcgtacaaat cggaagccga agccgatgcg gcgtggaaaa
     2401 cctattcgtc caagcatgcc ggcacgctgt ccggccgtgc caaggacgtg aagcaggtcg
     2461 atctcggcga aaagggaacg tggtatcgcc tgcgcgtcgg ctcgttcgcg gataaagcgg
     2521 cggcagcggc gttgtgctca aagttgaccg ccgaaggcgg cgcttgcttt ccggcgaaat
     2581 agaacaagcc gggaagggtg gggggaatgg aatgcgcacg cgtgcgatct atggatgcgc
     2641 gggaacgacg ctcgcatcgg aagaacaggc gttcttccgc gagacgcagc cgtggggatt
     2701 catcgtcttc gcgcgcaata tcgtcgatcc ggcgcagttg cgcacgcttg cggaaagcct
     2761 gcgcgaaacc gtgaacgatc cgcgcgcacc aattcttatc gatcaagagg gtggacgcgt
     2821 cgcgcggctg aagccgccgc attggcagga gcgtctggcg gcggcgcggt tcggagaact
     2881 ctatttgcgc gatcctgaag tggcacgcga agccgtttat ctgaacgcgc gcctgctcgc
     2941 gcacgacctt tgcgaggcgg ggatcaatgt cgattgcctg ccgctgctg
//
LOCUS       DASZMC010000342         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_135013,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000342 DASZMC010000000
VERSION     DASZMC010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135013"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VG274_07000"
     CDS             <1..703
                     /locus_tag="VG274_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HWE06437.1"
                     /translation="LSFQPESEVKRVISYAAEQGRKNFAALIPNTAYGKVSEGAFREA
                     VKASGGTVGLVEHFSPSAGATEQAGFVAKSGPDAVFIPQGGATLRTIAPALVSNGVDV
                     TSVKLLGTGLWDDYSTSREEALRDSWFAAPAPNADHSFVSEYRSAFGSQPPQLAALAY
                     DAMSLIALVGAGQPYHRFTKAALTDPNGFAGVDGIFRFDENGAIERGLSVLAVRPGGF
                     AVVSPAPTTFQGKGS"
     gene            complement(854..1990)
                     /gene="hemW"
                     /locus_tag="VG274_07005"
     CDS             complement(854..1990)
                     /gene="hemW"
                     /locus_tag="VG274_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWE06438.1"
                     /translation="MFGIYVHWPFCAAKCPYCDFNSHVRAKIEENAWIDGIVRELEYA
                     AALQADEQPLVSTIFFGGGTPSLMSGRAVGTVLDTIARLWRRTNDLEITLESNPASAD
                     AGRFADYRAAGVNRLSLGVQALNDADLKKLGRLHNVEEAKSALRLAQNTFDRVSLDLI
                     YARPDQTVEAWCSELKEALSFNTEHLSLYQLTIEPATPYATLYRTGALVIPDEDLAAA
                     LYETTQELTEAAGVPAYEISNHARVGAEARHNLLYWRYGSYAGVGPGAHGRLDLGDKR
                     YATSTERLPERWREKIVQHGHGLIEKSKITPEDAAREHLLMNLRLAEGIDLTAYRARW
                     GTAPDAARIAALAEQGLITLENDRLTATPRGRLVLNSVIAALAD"
     gene            complement(2140..2748)
                     /gene="rdgB"
                     /locus_tag="VG274_07010"
     CDS             complement(2140..2748)
                     /gene="rdgB"
                     /locus_tag="VG274_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWE06439.1"
                     /translation="MRLNRGDALVVASHNKGKVREIEELLAPFGFAVKGAAELGLVEP
                     EETEATFEGNAILKARTAAEVSGITALADDSGLSVTALDGAPGIYSARWAGESKDFTA
                     AMARVERELRGAKDRSAKFVCVLALASPGGEVETFRGEVEGTLVFRPRGARGFGYDPI
                     FVANGMTETFAEIDPAAKHAMSHRAKAFEKFVAALELKAKTK"
     gene            complement(2745..>3096)
                     /gene="rph"
                     /locus_tag="VG274_07015"
     CDS             complement(2745..>3096)
                     /gene="rph"
                     /locus_tag="VG274_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWE06440.1"
                     /translation="GGTRTASITGGYVALAQCIAYLKKTGMVTKPVIKDHVAAISCGI
                     VKGAALLDLDYDEDSTAETDANFVMTGGGGLVEIQGTAEGAPFSDEQFTELLRLARKG
                     IGELIEAQKRVTGG"
BASE COUNT          552 a          967 c          938 g          639 t
ORIGIN      
        1 gctaagcttc cagccggaga gcgaggtcaa gcgcgtcatt tcctatgctg cggaacaggg
       61 ccgcaagaat ttcgccgcac tgattccgaa cacggcctac ggcaaggtca gcgaaggcgc
      121 tttccgcgag gcggtgaaag caagcggcgg cacggtcggt ctggtagagc acttctcgcc
      181 gtccgcaggc gcaacggagc aggccggttt cgtcgcgaag tcgggaccgg acgctgtgtt
      241 cattccgcaa ggcggcgcga cactgcgcac gattgcgcct gcgctggtca gcaatggcgt
      301 cgatgtcacg agcgtcaaac tgctcggcac gggattgtgg gacgattaca gcacctccag
      361 agaagaggcg ttgagagata gctggttcgc ggcgccggcg ccgaatgccg atcattcgtt
      421 tgtctcggaa tatcgttccg ctttcggttc gcaaccgccg caactcgcag cgcttgccta
      481 tgatgcgatg tcgctgatcg cactggtagg cgcgggtcag ccctatcatc gcttcaccaa
      541 agcggcgctc accgatccca acggattcgc gggcgtcgat ggcattttcc gcttcgatga
      601 gaacggtgcc atcgagcgtg ggttgtcggt gcttgcggtc cgccccggcg gtttcgcggt
      661 cgtcagcccc gcgccgacga cgttccaggg caagggctcc taaagtagaa tctcttattt
      721 tgaatcacac ccttcactgt catcgcccgc tttatgcggg cgacccaatt tccctttttt
      781 gcagaatgga aaatgggttg cccggacaag ccggcaatga caaggaaagc ttcaattcga
      841 gaaaatcggg ctcctagtcc gccagcgccg cgatcacgct gttgaggacg agacgtccac
      901 gcggtgttgc ggtcaggcgg tcgttttcca acgtgatcag gccttgctcc gccagtgcgg
      961 cgatgcgagc cgcgtcgggc gctgttcccc agcgcgcgcg ataggcagtt aggtcgatgc
     1021 cctcggcaag acggagattc atcagcagat gctcgcgcgc cgcatcttcg ggtgtgatct
     1081 tgcttttctc gatcaggccg tgaccgtgct gcacaatttt ttcgcgccag cgctcgggaa
     1141 ggcgctcggt cgatgtcgca taacgcttat cgccgagatc gagacgccca tgcgcacccg
     1201 gcccgacacc ggcgtacgat ccataccgcc aatagagaag attgtgccgt gcctccgcgc
     1261 cgacgcgcgc atgattggag atttcataag ccggaacgcc tgcagcttcc gtcaattcct
     1321 gcgtcgtctc atagagcgcg gcggcgaggt cttcatcggg aatgaccagc gcgccggtgc
     1381 gatacagcgt ggcatacggc gtcgcgggct cgatggtgag ctgatagagc gacaagtgtt
     1441 cggtgttgaa cgacaacgcc tctttcaatt cgctgcacca cgcttcgacc gtttgatcgg
     1501 gccgcgcata gatcaggtcc agcgaaacac ggtcgaatgt attttgcgcc agccgtaggg
     1561 cacttttcgc ttcttcgaca ttgtgcagac ggccgagttt tttcaggtcc gcatcgttca
     1621 acgcctgcac gccgagcgac agacgattga cgcccgccgc gcggtaatcc gcaaaacgtc
     1681 ccgcatcggc gctggcagga ttggattcca gcgtgatctc cagatcgttc gtccgccgcc
     1741 acagccgcgc gatcgtatcg agcacggtgc cgacggccct gccgctcatc agcgatggcg
     1801 tgccgccacc gaaaaagatt gtgctgacga gtggctgctc gtccgcttgc agcgccgccg
     1861 cgtattcgag ttcgcgcacg atgccgtcga tccatgcgtt ctcttcgatc tttgcgcgga
     1921 catggctgtt gaagtcgcaa tacggacact tcgccgcgca gaacggccaa tggacataga
     1981 tgccgaacat ctagcctccc ccttgtgggg aggtcggaaa actttgagca tcgcgaaaag
     2041 ttttccgggt ggggggcggg actcgcggat ggagcggacg cccacccgaa gcgcatcgcg
     2101 cttcgacctc cccacaaggg ggaggtgaaa agagcgcatt catttcgttt tcgctttcaa
     2161 ctctagcgcc gccacgaatt tctcaaacgc ttttgcgcgg tggctcatcg catgcttggc
     2221 ggcgggatcg atctctgcga acgtctcggt catgccgttt gcgacgaaga tcggatcgta
     2281 gccgaaacct cttgcgccgc gcggtcggaa tacgagcgtc ccttcgactt cgccccgaaa
     2341 cgtctcaact tcgccgccgg gcgaagcgag cgccagcacg cagacgaact ttgccgatct
     2401 gtctttcgcg ccgcgcagct cacgttcgac acgcgccatg gcggcggtga agtctttgct
     2461 ctcaccggcc cagcgcgcgg aatagatgcc cggcgcgccg tccagcgctg tgacgctcaa
     2521 tccagaatcg tcggcgagtg cggttatgcc gcttacttca gcagccgtgc gcgctttcag
     2581 aatcgcgttg ccttcgaacg tggcctcggt ctcttccggt tcgacgaggc cgagttccgc
     2641 ggcgcctttc acagcgaagc cgaatggcgc gagcagttct tcgatctcgc gcaccttgcc
     2701 tttgttgtgg cttgcgacga ccagcgcatc gccgcggttg agcctcatcc gccggtcacg
     2761 cgcttctggg cttcgatcag ttcgccgatg cctttgcgcg cgaggcgcag caattcggta
     2821 aactgctcgt cgctgaacgg cgcgccttcg gccgtgccct ggatttcgac aagcccaccg
     2881 ccaccggtca tgacgaaatt cgcgtcggtc tctgccgttg aatcctcgtc gtagtcgaga
     2941 tcgagtaacg ccgcgccttt gacgatgccg caactgatcg ccgcgacgtg atccttgatg
     3001 acgggcttcg tcaccatgcc ggtcttcttc aggtaagcga tgcactgcgc cagcgcgaca
     3061 tagccgccgg tgatcgacgc ggtgcgcgtt ccgcca
//
LOCUS       DASZMC010000343         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1269022,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000343 DASZMC010000000
VERSION     DASZMC010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269022"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VG274_07020"
     CDS             complement(<1..1305)
                     /locus_tag="VG274_07020"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWE06441.1"
                     /translation="MLKNISVRGAREHNLKNVDVEIPRDTLTVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLELMQKPDVDHIEGLSPAISIEQKTTSRNPRSTVGTV
                     TEIYDYLRLLFARVGVPYSPATGLPIESQTASQMADRILALEAGTRLFLLAPIVRGRK
                     GEYRKELAELQKKGFQRVKIDGKYYELGQAPALDKKFKHDIEVVVDRIVVRPDLGNRL
                     PDSIETALNLADGLLVAEFADVKEKGEPRRLLMSSKFACPVSGFTIPEIEPRLFSFNS
                     PHGACPACDGLGTQLYFDLALIVPDESVSLRKGAIAPWSKSSSPYYLQTLEALARHYK
                     FSLNEPWEDLPKRVREILMNGSGEDEIRFIYDDGMRRYETRKTFEGVVPNMQRRFKET
                     DSSWIREELQRYQDTSPCEVCNGYRLKPEALAVKIAGRHIGEV"
BASE COUNT          279 a          463 c          497 g          291 t
ORIGIN      
        1 gacctcgccg atgtggcggc cggcgatctt caccgccaaa gcttcgggtt tcaaccgata
       61 gccgttgcag acctcgcagg ggctggtgtc ctggtagcgc tgcagctctt cgcggatcca
      121 cgacgaatcg gtctccttga agcggcgctg catgttggga acgacgccct cgaacgtctt
      181 cctggtttcg tagcgccgca tgccgtcgtc atagatgaac ctgatctcgt cctcgcccga
      241 tccgttcatc aggatttcgc gcacgcgctt cggcaaatcc tcccacggct cgttgagcga
      301 gaatttgtaa tgccgcgcca aagcttcgag cgtctgcaaa tagtagggcg acgaggattt
      361 ggaccacggc gcgatcgcac ccttgcgcag gctgacgctt tcatcgggca cgatcaatgc
      421 gagatcgaaa tagagctgcg tgccgaggcc gtcgcaggca ggacacgcgc catgcgggct
      481 gttgaacgaa aagaggcgcg gctcgatttc ggggatggtg aagccggaga ccgggcaggc
      541 gaatttcgac gacatgagga ggcgccgggg ctcgcccttt tccttcacgt cggcgaattc
      601 cgcgacgagc aggccgtcgg caaggttgag cgcggtctcg atcgaatcgg gcagccggtt
      661 gccgagatcg ggccggacca cgatgcgatc gacgaccacc tcgatgtcgt gcttgaattt
      721 cttgtcgagc gccggcgcct ggccgagctc gtaatatttg ccgtcgattt tgacgcgctg
      781 gaagcccttt ttctgcagct ccgccagttc cttgcggtat tcgcccttcc gtccgcgcac
      841 gatcggggcg agcaggaaga gccgcgttcc ggcttcgagc gccagaatgc ggtccgccat
      901 ctggctggcg gtctggctct cgatggggag cccggtggcg ggcgaatagg gcacgccgac
      961 ccgcgcgaac aggaggcgca gatagtcgta aatctcggtg accgtgccga cggtggagcg
     1021 cggattgcgc gacgtcgtct tctgttcgat ggaaatcgcc ggcgagagcc cttcgatgtg
     1081 gtccacgtcg ggcttctgca tcagctccag gaactggcgc gcataggcgg aaagactctc
     1141 gacgtagcgc cgctggcctt ccgcatagat ggtgtcgaac gccagcgacg attttcccga
     1201 gccggacaga ccggtgatga ccgtcagcgt gtcgcgcggt atctccacat cgacgttctt
     1261 gaggttgtgc tcgcgggcgc cgcgaacgga aatgttcttc agcatgacat ccaatccaaa
     1321 cgcccagttt cgccgacgcg gtgcgccccg gcaaccacgt gtccgccttc tgctgcccgg
     1381 atggcagccc ggccacgaaa ggttgaagga ggcctcccat cgcagccgcc gaaaaagcgt
     1441 tccacggccc gcgattcccg atggaacgta ggtagaacgg aggcaagttg caacgggtgg
     1501 gagccgcgag aaaaggtttt aacgcagcgg
//
LOCUS       DASZMC010000344         6652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1269115,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000344 DASZMC010000000
VERSION     DASZMC010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269115"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VG274_07025"
     CDS             complement(<1..495)
                     /locus_tag="VG274_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWE06442.1"
                     /translation="MSILPGTTGRRRLYLMRHGHVDYFAPKIATIGGIRDISLTPQGR
                     DEAEAAALALSAVVLDRVFCSGLPRARQTAEIVVEHQARLQNIEAESALSEIRGGRLL
                     DVSNRSELIATMVFHFARAGEDGATMLEGGEAFAGAQERAVGVVRRILGESGWRQILI
                     VAHEG"
     gene            complement(504..1622)
                     /locus_tag="VG274_07030"
     CDS             complement(504..1622)
                     /locus_tag="VG274_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWE06443.1"
                     /translation="MGPLAGIRVVEFAGIGPAPFAAMLLSDMGAEVIRIDRKGGRTPR
                     STDVTFRGRRAIAFDMKKTEAVDATLRLVEQADALIEGFRPGVMERLGIGPEVCLERN
                     PRLVYGRMTGWGQSGPLARAAGHDINYIALTGALHAIGSAGGKPVPPLNLAGDFGGGA
                     LYLAFGLLCGIVEAMRSGKGQVVDAAMIDGAASLMSMFYGMAASGMWQDERGVNLLDS
                     GAYFYDTYETKDGKWIAIGALEPQFHREFLDRIGLADLDASLQMDRENWPEFRSRVAS
                     AIKAKTRDAWDAALLGTDACYAPVLSLAEAPAHPHNIERQTFTKIDGVVQPAPAPRFS
                     RTPPEVQGPPQAPQSALVLSSWGFSTAEIDRLHAAEAI"
     gene            complement(1755..3125)
                     /locus_tag="VG274_07035"
     CDS             complement(1755..3125)
                     /locus_tag="VG274_07035"
                     /inference="COORDINATES: protein motif:HMM:NF007135.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HWE06444.1"
                     /translation="MTARVLVVDDILSNVKLLDAKLTAEYFEVETASSGAEALDKIAA
                     NEPDIVLLDVMMPGMDGFEVCRRIKANPATAHIPVVMVTALDQSSDRVAGLGAGADDF
                     LTKPVDDEALLARVRSLVRLKMLTDELRMREATGKSMGLLDPAETLSEERPTGQILLI
                     EDRHEPAAWMSAALTPDNQLLSVETFEEALVRVREGDFDLIIVSLGLRGFDGLRLCSQ
                     LRSLPEGRNIPILVLVSAGDRRKLNQALEMGVNDYLARPLDRNELVARVRTQLRKKRY
                     ADRLRQNAHLSVEMALTDQLTGLHNRRYMTRHLATLLASAAGSGRHLAFLIIDVDHFK
                     SVNDSFGHDIGDEVLREFASRISENVRGIDLACRFGGEEFVVVMPDTDVSHAWTVAER
                     LRKSVEATPFGVSRAPGTLNITISIGIAGSEGSGDTAEALLRRADQALYRAKRDGRNR
                     VVADAA"
     gene            complement(3131..3505)
                     /locus_tag="VG274_07040"
     CDS             complement(3131..3505)
                     /locus_tag="VG274_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE06445.1"
                     /translation="MDAKSKTVLIVEDNELNMKLFHDLLDAHGYNILQTKDGMEALDI
                     AREHHPDLILMDIQLPEISGLEVTKWLKEDDSLKAIPVVAVTAFAMKGDEEVIRQGGC
                     EAYISKPISVTGFLDTVRRFIG"
     gene            3625..3906
                     /locus_tag="VG274_07045"
     CDS             3625..3906
                     /locus_tag="VG274_07045"
                     /inference="COORDINATES: protein motif:HMM:NF023518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HWE06446.1"
                     /translation="MPGNLDDSEVLALNALTFLVKSPVEMERFFAISGIGPGDLGRVA
                     GDAEFLGGVIDFLLGDDALLVRFCDFHSLDPRAVHVARHELAARARSRD"
     gene            3907..4836
                     /locus_tag="VG274_07050"
     CDS             3907..4836
                     /locus_tag="VG274_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWE06447.1"
                     /translation="MAMRLGVDLGGTKIEIIALDGRGHTPLRERISTPTSGYDAIVAA
                     IGDLVLSAEAKLGCRGTVGLAIPGALSPRTGLVKNANTIALNGRPLDADLSNALGRRV
                     RVANDANCFTLSEATDGAAAGCNVAFGIIAGTGVGGGICVGGRLVEGAHLIAGEWGHN
                     PLPSPLATEIPGPACYCGRHGCIECWISGPALAREFARRTGRSLPPEAIAKAALGGDA
                     AAIAAMSQYIDRFARSLALVVNILDPDAIVLGGGLSNIDMLERELPERVEAYSFSPEV
                     PPRIVRNFHGDSSGVRGAAWLWREDEISAGLPS"
     gene            complement(5072..5938)
                     /gene="aguB"
                     /locus_tag="VG274_07055"
     CDS             complement(5072..5938)
                     /gene="aguB"
                     /locus_tag="VG274_07055"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR03381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HWE06448.1"
                     /translation="MRNVTFAATQFASSPDSKANIARAKELVREAAGQGANVVLVQEL
                     FETPYFCQDQNPEHFQLASEFEGNALVHEMAAQARELGVVLPVSFFERAGHAHFNSLA
                     MVDADGAVLGLYRKSHIPAGPGYQEKFYFTPGDTGFRVWRTRFGMFGCGICWDQWFPE
                     AARAMALQGAEALFYPTAIGSEPPPAPPLDSRDHWRRVMQGHAAANCMPLVASNRTGR
                     ERGEAGEMTFYGSSFIADATGALVAELGREDEGVIVATFDLDETARARASWGLFRDRR
                     PDLYNPLTRHGA"
     gene            complement(5935..>6652)
                     /locus_tag="VG274_07060"
     CDS             complement(5935..>6652)
                     /locus_tag="VG274_07060"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HWE06449.1"
                     /translation="AERAGIAWQFNAWGRKYHPFENDQAFAGRVLDHVRARTYRPSLV
                     CEGGAIHADGDGTLMTTEQCLLNSNRNPSLSKEQVEDILARYTGCRRVLWLGSGFSDE
                     ETDGHVDNIACFAAPGRVIIGIPPLRSHPDFAAVMEAKRRLANTRDAEGRQIEIIEIA
                     QPRRMRMDWRGRLLAASYVNFYLVNGGVVMPGFDDLEDGPARRVLADCFPGRDIVQVD
                     ALDIVEGGGGLHCITQQEPA"
BASE COUNT         1231 a         2109 c         2053 g         1259 t
ORIGIN      
        1 cccttcatgc gccacaatca ggatctgccg ccagccggat tcgccgagga tgcgccgaac
       61 gacgccaacc gcacgttcct gggcgccggc gaaagcttcc ccgccttcca gcatggttgc
      121 tccatcctcg ccggcgcggg caaagtggaa caccatcgtg gcgatcagct cgctgcggtt
      181 ggaaacgtcg agcagcctgc caccgcggat ttccgacagc gcggactcgg cttcgatatt
      241 ttgcagccga gcctggtgtt caaccacgat ttccgcggtt tgccttgctc gcggcagacc
      301 tgagcaaaaa acccgatcga gcaccaccgc ggacagagcg agcgcggcgg cctcggcctc
      361 gtcgcgtccc tgcggcgtca gggagatatc ccgtatgccg ccaatcgtcg cgattttggg
      421 tgcgaaataa tcgacgtgcc catgtcgcat cagatagagg cggcggcgtc ccgtcgtgcc
      481 aggcaatatg ctcaaggacg cgcctatatc gcttccgcgg cgtgaagccg gtcgatttcc
      541 gcggtggaaa atccccagct ggacagcacc agcgcgcttt gcggagcttg cggcggcccc
      601 tgcacctccg gcggggttcg gctgaagcgc ggcgccggcg ccggctgcac cactccgtcg
      661 attttggtga aagtctgccg ctcgatattg tgaggatgcg cgggcgcttc ggcgagcgac
      721 agcaccggcg cgtaacatgc atcggttccg agcagcgctg catcccacgc gtcgcgcgtc
      781 ttcgccttta tcgcgctagc caccctcgat ctgaactcag gccaattttc tcggtccatc
      841 tgcaggctcg catcgagatc ggcgaggccg atccggtcga gaaactcgcg atggaattgc
      901 ggttcaagcg ccccgatggc gatccatttt ccgtccttgg tctcgtaggt atcgtaaaaa
      961 tacgcgcccg aatcgagcag gttcactccg cgttcgtcct gccacatccc ggacgcggcc
     1021 atgccgtaga acatcgacat cagcgaagcg gcgccgtcga tcatcgctgc gtcgaccacc
     1081 tgcccctttc cggagcgcat ggcttcgaca attccgcaca gcaatccgaa cgcgaggtag
     1141 agagcgccgc cgccgaaatc gcccgccaga ttgagcggtg gaaccggctt tcccccggca
     1201 gaaccaatgg cgtgcaacgc gcctgtcaac gcgatgtaat tgatgtcgtg gcccgcggcg
     1261 cgcgcgagcg ggcccgactg cccccaaccg gtcattcgcc cgtaaacgag ccgcggattg
     1321 cgttcgagac agacttccgg gccgatgcca aggcgctcca tgacgccggg acggaaaccc
     1381 tcgatcagcg catccgcctg ttcgaccagc ctgagcgtgg catcgacggc ctcggtcttt
     1441 ttcatgtcga atgcgatggc gcgccggcca cgaaacgtca cgtccgtgct gcgaggcgtg
     1501 cggcctccct tgcgatcgat gcggatcacc tccgcaccca tgtcggacag aagcatcgcg
     1561 gcgaaaggcg ccggaccaat tccggcaaat tccaccaccc ggattccagc gagtggcccc
     1621 acggatactg cgcccttcgt ctttgcatgc ccctcttacc tgccggcaag cggcgagtaa
     1681 acgacaccgc gcgcaggcag cgcggagggg tcaagcgggc gagaagttgt gggttgttta
     1741 cgccgcgacc gtccttacgc cgcgtccgcg accacgcgat tgcggccgtc gcgcttggcg
     1801 cgatagaggg cctggtcggc gcgacggagc agggcttcgg cggtatcgcc agatccctcg
     1861 gagccggcaa taccgatgct gatggtaata ttaagcgtgc ccggcgcgcg gctgacgcca
     1921 aacggcgttg cttctacgct cttgcgcagc cgctcggcca cggtccaggc atgagagaca
     1981 tccgtatccg gcatgacgac gacgaactct tcgccgccga agcggcaggc aagatctatc
     2041 ccacgaacgt tttcggagat ccggctcgca aattcgcgaa gcacttcatc gccgatgtcg
     2101 tgcccgaaag aatcgttgac cgacttgaag tgatcgacgt cgatgatcag gaatgccaga
     2161 tgccttccgg agccggccgc actcgccagc agcgtagcca gatggcgcgt catgtagcgg
     2221 cggttgtgga gaccggtgag ctggtcggtc aaagccattt ctacggaaag atgagcgttc
     2281 tgtctcaggc ggtcggcgta gcgcttcttg cgaagctggg tgcgtacgcg cgctacgagc
     2341 tcgtttcggt ccagcggccg ggcgagatag tcgttgacgc ccatttccag cgcctggttc
     2401 agcttgcggc ggtcgcccgc gctgaccagc acgagaatgg gaatgttgcg cccttccggc
     2461 aaggagcgga gctgagaaca caggcgcaat ccatcgaagc ccctcaaccc aaggctgacg
     2521 atgatgagat cgaaatcgcc ttcacgcacc cgcaccaggg cttcttcaaa cgtttcgacc
     2581 gataaaagct ggttatcggg tgtcagcgcc gccgacatcc aggcagcagg ttcgtggcga
     2641 tcctcgataa gaagaatttg accggtggga cgctcctcgg aaagggtttc cgcggggtcg
     2701 agcagtccca tgctcttgcc ggttgcttct ctcatccgca gctcgtccgt taacatcttg
     2761 agccgcacga ggctgcgcac gcgtgcgaga agcgcctcgt catcgacggg tttggtcaga
     2821 aagtcgtcgg cgcccgcgcc caggccggca acacggtcgc tgctctgatc gagggctgtg
     2881 accatcacga cgggaatgtg ggcggtcgca ggatttgcct tgatgcgacg acagacttcg
     2941 aaaccgtcca ttcccggcat cattacgtcc agcaacacga tgtcgggttc gtttgcggcg
     3001 attttgtcca gcgcttcggc gccgctggac gccgtctcca cctcgaaata ctcggcggtg
     3061 agcttggcgt ccaggagctt tacgttggag aggatatcgt cgacgacaag aactctcgct
     3121 gtcatggcga tcagccgatg aagcgccgta cggtatcgag aaaacccgtg accgagatcg
     3181 gtttggaaat gtaggcttcg cagccgccct gccgaatgac ttcttcatcg cccttcatcg
     3241 caaaggccgt gaccgcgacg acggggatgg ctttgagcga atcgtcttcc ttgagccatt
     3301 tggtgacttc caggcccgaa atctcgggca gctggatgtc catgagaatg agatcggggt
     3361 ggtgctcgcg ggcaatatcc agtgcttcca tgccgtcctt ggtctgcagg atattgtagc
     3421 cgtgagcgtc gagcaggtcg tgaaagagct tcatgttcag ctcgttgtct tccacgatca
     3481 gcactgtctt cgatttggca tccatgcttt attctcccag cccgccgcac accgatcccg
     3541 cgtcccgcgt gcgccagttc cgcgagcaac gtcaccaatg acaattacta agccacatat
     3601 cttaacgctc ccttgacgat gcccatgccc ggcaacctgg acgactccga agtattggcg
     3661 ctcaacgcct tgacattcct tgtcaaatcg cccgtcgaga tggagcgatt ctttgcaatc
     3721 tcgggaatcg gtccgggcga tctcggccgg gttgccggcg acgccgagtt tctcggcggc
     3781 gtgatcgact ttctgctcgg cgacgatgcg ctgctcgtgc gtttctgcga cttccattcg
     3841 ctggacccgc gcgccgttca tgtggcgcgg cacgagctcg ccgctcgagc gcgaagccgg
     3901 gattagatgg cgatgcggct cggcgtcgat ttgggcggca cgaagatcga gatcatcgcg
     3961 ctcgacggac gcggtcacac accactgaga gaacggattt cgacgcccac atccggatac
     4021 gatgcaatcg ttgccgcgat cggcgatctt gtcctttctg ccgaagcgaa acttggctgc
     4081 cgcggcacgg ttggcctagc catccccggc gccctgagcc cgcggacagg cctggtcaag
     4141 aatgccaaca caattgcgct caacggccgt cccctcgatg cggatttgag caacgcgctc
     4201 ggccgccgcg ttcgggtcgc caacgacgcg aactgcttca ccctctcgga agcgacggac
     4261 ggcgcggccg ccggctgcaa cgtcgctttt ggcatcattg ccgggacagg tgtcggcggc
     4321 ggaatctgcg tgggtggacg cctcgtcgaa ggcgcgcatc tgatcgccgg cgaatggggg
     4381 cacaatcccc tgccctcgcc tctcgccacc gaaattcccg gaccggcctg ctattgcggc
     4441 cggcacggct gcatcgaatg ctggatcagc ggtcccgcgc ttgcccgcga attcgcgcgt
     4501 cgcacgggcc gctcgctccc gcccgaggcg attgccaaag ctgcgctggg cggcgacgca
     4561 gcggcaattg ccgcgatgtc gcaatatatc gatcgattcg cccgttcgct cgcgctggtt
     4621 gtcaatattc tcgatccgga cgccatcgtg ctcggcggcg gcctgtccaa catcgatatg
     4681 ctggaacgcg aactgccgga acgcgtggag gcctacagtt tctcgccgga agttcccccg
     4741 aggatcgtgc gaaattttca cggggattcg agcggcgtgc ggggagcggc gtggctctgg
     4801 cgcgaagacg agatatcggc cggcctgccg agttgaatac gacggccgcg gatggcgaaa
     4861 ccgggcgatt gaggcaagag aacgtaagtt agatcgtgat tcatttgcat tgaaccaaaa
     4921 atgtcgtccc cgggggagag cgccaaaatg tttcctcccc cgcgcttgcg cgggggagga
     4981 aacactccgc atcggcagtg ggaacatagg gaagcgcttg tgggggattg cacgacggag
     5041 gtagtgcgat tcaatgcgac gggatcgtgt tctacgcgcc atgccgcgtg agggggttgt
     5101 acaaatccgg cctgcggtcc cggaagaggc cccacgacgc gcgcgcgcgc gccgtctcgt
     5161 ccaggtcgaa cgtagcgacg atgacgccct catcctcgcg cccaagctcg gcgacgagag
     5221 cgccggtcgc gtcggcaatg aatgacgatc cgtaaaacgt catctcacct gcttcgcctc
     5281 gctccctgcc ggtgcggttg gatgcgacga gcggcatgca attggccgcc gcatgcccct
     5341 gcatgacgcg gcgccagtga tcgcggctgt cgagcggcgg cgccggcgga ggctccgagc
     5401 cgatcgcggt gggatagaac agcgcctccg ccccttgcaa agccatcgcg cgcgcggctt
     5461 cgggaaacca ctgatcccag cagatgccgc aaccaaacat tccgaagcgc gtccgccaga
     5521 cgcggaagcc ggtatctccg ggcgtgaaat agaatttttc ctggtatccg ggaccagcgg
     5581 gaatatgcga tttccgatac agcccgagaa cggcgccgtc cgcatcgacc attgcgagcg
     5641 aattgaaatg cgcgtggccg gcgcgctcga agaaactgac gggcagcacc acgcccagct
     5701 cgcgggcctg cgccgccatt tcgtgcacca gggcgtttcc ctcgaattcg gacgccagtt
     5761 gaaaatgctc gggattctgg tcctgacaga aatagggcgt ttcaaagagc tcctggacga
     5821 gaacaacatt ggcgccctgg ccagctgctt ctcgcaccag ttccttggcc ctcgcgatat
     5881 tggctttgga atcgggagag gatgcgaact gcgtcgcggc gaacgttacg tttctcatgc
     5941 cggctcctgc tgggtgatgc agtggagtcc cccgccgccc tcgacgatgt ccagcgcgtc
     6001 aacctggacg atgtcgcggc ccggaaagca gtcggcaagc acccggcgcg ccggaccgtc
     6061 ttccagatcg tcgaacccag gcatgacgac gcctccgttc acgagataga aattgacgta
     6121 gcttgctgca agcagccggc cgcgccagtc cattctcatt cttctcggct gcgcaatttc
     6181 gataatttcg atctgtcgcc cttccgcatc gcgcgtgttc gcaagccggc gttttgcttc
     6241 catgacggca gcaaaatcgg gatgggacct gagaggagga attccaatga tcacccgtcc
     6301 aggagcggcg aagcaggcga tattgtcgac gtggccatcg gtttcttcgt ccgaaaagcc
     6361 gctgccgagc catagaacgc gacggcagcc cgtgtatctg gcaaggatgt cttcgacctg
     6421 ttccttcgaa agtgagggat tgcggttcga attgagaaga cactgctcgg tcgtcatcag
     6481 cgtcccgtcg ccatcggcgt gaatggcgcc gccttcgcag accagcgacg gccgatatgt
     6541 ccgcgcgcgg acgtgatcga gaacgcgccc cgcgaacgct tggtcgtttt cgaacggatg
     6601 gtactttcga ccccaggcat tgaactgcca tgcgatcccg gcgcgttccg ca
//
LOCUS       DASZMC010000345         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5367087,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000345 DASZMC010000000
VERSION     DASZMC010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5367087"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VG274_07065"
     CDS             <1..186
                     /locus_tag="VG274_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWE06450.1"
                     /translation="GVETVRQYLRAGLIDELHLALSPVVLGQGEAMFAGIDLPALGFR
                     VTEHQATADATHIVLSR"
     gene            complement(218..1261)
                     /locus_tag="VG274_07070"
     CDS             complement(218..1261)
                     /locus_tag="VG274_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWE06451.1"
                     /translation="MKQNFTVRRGALDGVEAFLSVAHHRNFRKAAAELAVTPSAISQA
                     VRALEARVSAALFIRTTRSVGLTEAGERFLSRAKPAFDELVAASEAARDLGQRPAGLL
                     RLSVPRAVVPLILEPVIASFCQAYPEIELEIAASEEMVDVAAGGFDAGIRLGQFIAPD
                     MVAVRLTPPFPLVVVGSRDYLRRRKPPQCIDDLRHYACLRMRRSNGSIAPWSFIDGNK
                     AVEAIVSGPLIAHDYPTLLGAAIQGLGLAQLPDPLAKAPIGDGRLHALLTRFAVGTPG
                     VFLYYPNRRQVLPKLRAFIEHIKNQSAGAPHGRTSARRVDKRRGADARDVRHAILRPE
                     RMTRLAFVSGCFP"
     gene            1389..1733
                     /locus_tag="VG274_07075"
     CDS             1389..1733
                     /locus_tag="VG274_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06452.1"
                     /translation="MSVETNPSGAPAQPVSPTITAVLVILTAKTGVKREQIMSVMPAE
                     IRETVKLYLNGTIREWYSRGDGRGAVFLLDTGDVAEAHTIMESLPLAKTNLLDHEYIA
                     VGPLFPLRLLMA"
     gene            1778..2320
                     /locus_tag="VG274_07080"
     CDS             1778..2320
                     /locus_tag="VG274_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06453.1"
                     /translation="MSPATIFRVHLILGYVAWLLCLGAYIWPKLKSMNRAEAQRAIAT
                     LHSFRFFGLVFILPGVVGPDLPAGFTVFAAYGDFATGLLAMLALLTIRARFFFWPLVL
                     AYNVVGVADIFIDYYHGIVLDLATVSGQLGATYVIPIIYVPILMITHVAALYLLARPQ
                     SKEAPDLADGARAVDGISRV"
BASE COUNT          511 a          836 c          815 g          546 t
ORIGIN      
        1 ggcgtcgaga cggtgcgtca ataccttcgc gcgggattga tcgacgagct gcatttggcc
       61 ttgtcccccg tcgtgctcgg gcaaggtgaa gcaatgttcg ccgggattga cctgccggcg
      121 ctcggcttcc gcgtcaccga acaccaggcg accgcagacg ccacccatat cgtcctcagc
      181 cgataggagc ccaccaccga caatcggagc ttctgtttta cggaaaacac ccgctgacga
      241 acgcaagtct ggtcatgcgt tccggtcgta ggatagcgtg gcgtacatca cgagcgtctg
      301 caccgcggcg cttgtcgacc ctccgcgcgc ttgttcggcc gtgcggagcg ccggcgcttt
      361 ggtttttgat gtgttcgatg aacgcgcgca gtttgggcaa aacttggcgc ctgttcgggt
      421 aataaagaaa aacgcctggc gttccgacgg caaagcgcgt cagcaacgcg tgcaaccggc
      481 cgtcgccgat cggcgccttg gcgagtgggt cgggcagctg cgcaagtccc aaaccctgga
      541 tcgccgctcc gaggagggtg gggtagtcgt gcgcgatgag cggtcccgac acgattgcct
      601 cgacagcctt gttgccgtcg ataaaggacc agggcgcgat cgacccgttt gagcggcgca
      661 tgcgcaggca agcgtaatga cgcagatcat cgatgcattg tggcggcttt cgccggcgta
      721 agtagtcgcg gctgccaacg accaccaacg ggaacggtgg cgtcagccgc accgcgacca
      781 tatcgggtgc gatgaactgg ccaagacgga taccggcatc gaagccgcct gccgccacat
      841 ccaccatctc ctcgctcgcg gcaatctcta gttcgatttc gggataggcc tggcaaaaag
      901 aggcgatcac cggctcaagg atcagcggga cgaccgcgcg cggcaccgaa aggcgcaata
      961 ggccggcagg ccgctgcccg aggtcacgcg cggcttcgct ggcggcgacg agctcgtcga
     1021 aggcgggctt cgcgcgcgac aaaaaccgtt cgccggcttc ggtcagaccg acgctgcgcg
     1081 ttgtgcggat gaagagtgcc gcgctgaccc gcgcttcaag tgcgcgcacc gcctggctga
     1141 tcgccgacgg agtcacggcg agttccgcgg ccgctttacg gaaattgcgg tggtgggcga
     1201 cgctcaggaa cgcttccacg ccgtcgagcg ctccccgcct gactgtgaag ttctgcttca
     1261 tagcctgtcg acattatcac ggatagtcgc tcgcgcgaaa gcagcctaga tgtgcagtga
     1321 cgtcgatgcg aaacccaaga tccgcgacat cgtgtgcttt cacgatcatt agacataagg
     1381 agtctgccat gagtgtcgaa accaatccgt ccggcgcccc ggcgcaaccc gtctcgccca
     1441 cgatcacggc ggtgctcgtc atcctcaccg ccaagacggg cgtcaagcgc gaacagatca
     1501 tgagcgtcat gccggccgaa atccgcgaga ccgtgaagct ctatctcaac gggacgattc
     1561 gtgaatggta ttcgcgcggc gatggtcgcg gagctgtttt cttgctggat accggagatg
     1621 ttgccgaggc gcacacgatc atggaaagcc ttccgctggc caagaccaat cttctcgacc
     1681 acgagtacat cgctgtcgga ccactcttcc cgcttcgctt gctcatggcc tagcgcaagc
     1741 aacaaggacg ggcgccaata ctgacgagag agatcacatg tcgccagcaa ccatcttccg
     1801 agtccatctc attcttggct acgtcgcctg gctgctgtgc cttggcgcat acatctggcc
     1861 caagctcaag tccatgaacc gggccgaagc gcagcgtgcc atcgcgacgc tgcacagctt
     1921 ccgcttcttc gggctcgtct ttattctccc gggcgtcgtt gggcccgatc taccagccgg
     1981 cttcacggta ttcgcagctt acggcgactt cgcaaccgga ttgctggcca tgctcgcgct
     2041 cctcacaatc agggcgcgct tcttcttctg gcctctggtc ttggcttaca atgtggtggg
     2101 agtggccgac atcttcatcg attattatca cggtatcgtg ctcgatcttg cgacggtgtc
     2161 gggacagttg ggcgccacct acgtgattcc gatcatctat gtgcccattt tgatgatcac
     2221 gcatgtcgcc gcactctatt tgctggcgcg gcctcaatcc aaagaagcgc cggacctcgc
     2281 cgacggtgcg cgcgcggttg acggtatttc ccgcgtctga gcggctgatg ctctacgcgt
     2341 ccgcatcgcg aaaatcgacc aatcgacgcc gatcgtcagc gaagccgagc aaatcgcggc
     2401 tcgtcgaaat tccctttcga aatcaaaagt gcaaatttct gacggatcgg gagtcggtgc
     2461 ttggcgatgc gacgaaaaat tctgtctcgc tcgatcaata cgttaccgtc gctcgaacaa
     2521 tcgagtggga gtgagggggc aaccggcggc gaccgcgtag tttcggtggc gattttcgct
     2581 gacgtgctgc tgctctgtcg atcccttcgt cactggctcg gaggctcttc tatgtccgtg
     2641 ttctcgggta gctcgcagaa ccgatctgcg cgcaatccgc ggaatatgta aagtgccttc
     2701 aacgggtg
//
LOCUS       DASZMC010000346         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3702540,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000346 DASZMC010000000
VERSION     DASZMC010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3702540"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VG274_07085"
     CDS             complement(<1..701)
                     /locus_tag="VG274_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HWE06454.1"
                     /translation="MNNLSRLALVAAFLGVTGTAAAASTEVVLHSFKGSQHRDGSMPQ
                     SDLIADAAGNLYGTTQKGGKRGGSGSGTVFELSPTGDPDVWTETVLYDFKAGSDGDGP
                     VGGLLMDAKGALYGTTENGGTMNDGTVFKLSPPKTAGDNWKEHVLYSFCSAISCADGA
                     HPIAGLTLGKKGVLYGTTQFGGAGAFGPQEGTAFQLTPPSTKGGAWTETVLHNFCDLA
                     NCADGFEPNPGRLLRT"
     gene            851..>1941
                     /locus_tag="VG274_07090"
     CDS             851..>1941
                     /locus_tag="VG274_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE06455.1"
                     /translation="MSVENGGNARRLTTWKEIATFLGRDERTVKRWEESRGLPVRRVP
                     GSGRASVFAYAHELQAWLDGTQPEPAAVPPPAARPKFAIAVTALAVVLAVAGAATWFL
                     NLPPWPSRAPVRDPVAVVLYRSGLHEWQTRTPSGLTRAVKDFNAAIARDPTYAQAYEG
                     LAETYNLMREYTSMPADEAYAKARDAATRAIALDPSLAGAHAALAFVDFYWLRKVPDA
                     RREFARAVELDPKNAEARHWYATFLMTVGEFDAALREIEKASELDSESSAILSDKGLI
                     LYYAGRTGDALKLLAQMETDQPELASPPFYLSVIDLVRGDNESHLREMKHHAVVTHDA
                     GEAALAVAGAKGLAQSGRAGMLSGELSARL"
BASE COUNT          348 a          602 c          630 g          361 t
ORIGIN      
        1 gtgcgcagca ggcggcccgg gttcggctcg aatccgtcgg cgcaattcgc gaggtcgcag
       61 aaattgtgca gcacggtttc cgtccaggcg ccgccttttg tcgatggcgg cgtcaactgg
      121 aaagccgttc cttcttgcgg accaaaggcg cctgcgcctc cgaattgggt tgtgccgtaa
      181 agcacgccct tctttcccaa agtcagtccc gcgatcggat gcgcgccgtc cgcgcagctg
      241 atggcgctgc aaaagctgta gagaacatgt tctttccagt tatcgcctgc cgttttgggc
      301 ggcgaaagct tgaacacggt gccgtcgttc atggtgccac cattctcggt ggtcccgtac
      361 agcgcccctt tggcgtccat caggaggccg ccgaccgggc catcgccgtc gctgcccgct
      421 ttgaaatcgt agaggactgt ctcggtccag acgtctggat ctccggttgg gctgagttcg
      481 aagacggttc ccgacccgct gccgccgcgc ttcccgccct tctgtgtggt gccgtagaga
      541 ttgccggcgg catcagcgat cagatcgctc tgcggcatgc ttccatcccg atgctgcgag
      601 cctttgaaac tatgcaggac gacttccgtc gaagcggctg ccgccgtacc ggtaacgcca
      661 aggaatgcgg cgacgagcgc caaacgcgac aggttgttca taattttctc tcacccaaat
      721 gtgacaggaa cgtcgcggcc ggcttcggcc ggacgcatgc tttgggctat gatcagcccg
      781 cgcccagaag tcggcgcatt cgcggaatgg cgcaaaaggc gtattggggc agttcggggc
      841 aaatgaggac atgagcgtcg aaaacggcgg aaatgcccgg cgtctgacga cgtggaagga
      901 gatcgccacg tttctcgggc gcgacgagcg cacggtcaaa cgctgggaag agtcgcgcgg
      961 cctgcctgtc cgccgggtgc cgggcagtgg ccgcgcttcg gtctttgcct atgcccatga
     1021 acttcaggca tggctcgacg gcacgcagcc ggagccagcg gcggtgccgc cacctgccgc
     1081 aaggccgaag tttgcaatcg cagtcacagc actggccgtc gtgctcgcag tcgcgggggc
     1141 agcaacctgg ttcctcaatc ttccgccctg gccttcgcgt gcgccagttc gcgacccggt
     1201 ggcggtggtg ttgtaccgat ccgggctgca cgagtggcaa acacgcacac cgtccggatt
     1261 gacgcgcgcc gtgaaggatt tcaacgcggc gatcgcgcgc gatccgacct acgcgcaggc
     1321 ctatgagggg ctggcagaaa cctacaatct gatgcgcgaa tacacgtcga tgccggcgga
     1381 cgaagcttat gcgaaagcgc gcgatgcggc gacgcgtgcg attgcgctcg atccgtcgct
     1441 ggcgggcgcg catgcggcgc tcgcgtttgt cgatttctac tggctgcgca aagtgcctga
     1501 cgcaaggcgc gaattcgcac gcgccgtgga actcgatccg aagaacgcgg aagcgcgtca
     1561 ttggtacgcg acgttcctga tgacggtcgg cgaattcgac gcagcgctgc gcgagatcga
     1621 aaaagcgtcg gaactggatt ccgaatcctc ggcgatcctc tcggacaagg gcctgatcct
     1681 ctattacgcc ggccgcaccg gcgatgctct gaaattgctg gcgcagatgg agacggacca
     1741 gcccgaactg gcatcgccgc cgttctatct ttcggtcatc gatctcgtgc gcggcgacaa
     1801 cgaaagtcat ctgcgcgaga tgaagcacca tgccgtcgtg acacatgacg caggcgaagc
     1861 ggccctggct gtggcgggcg ccaaaggcct cgcgcaaagc ggccgcgccg gaatgttaag
     1921 cggcgagctt tcggcgcggc t
//
LOCUS       DASZMC010000347         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_718875,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000347 DASZMC010000000
VERSION     DASZMC010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718875"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..3669
                     /gene="icmF"
                     /locus_tag="VG274_07095"
     CDS             385..3669
                     /gene="icmF"
                     /locus_tag="VG274_07095"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF045497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HWE06456.1"
                     /translation="MNDVTTARRDKIERPLPTQGPPHTTRNKIRFVTATSLFDGHDAA
                     INIMRRILQASGAEVIHLGHNRSVDEVVTAAIQEDVQGIAVSSYQGGHVEYFKYAVDL
                     LHERGAGRIKIFGGGGGVITREEMDELHAYGVERLYSPADGQRLSLQGMIDDMMARAD
                     FDPADDGAVEVRTLATPGWRPLARFLTRLEHGRVDAATLTNLTGNRDKRRAMPVLGIT
                     GTGGSGKSSLCDELIRRFRLDSEEFRIAVLAIDPSRRKSGGALLGDRIRMNAINAPQI
                     YMRSIATRDSESEVPQFIPAAVEAARAAGFDLVIVETPGIGQGNTAIADLADISLYVM
                     TPEFGAASQLEKIDMLDFADLVAINKFDRRGAEDALRDVRKQVQRNRQFFNRPPESMP
                     VYGAIAARFQDDGVTGLYLGLRDLLVEKGFHPPPSRFDAKSDRVPSPRQAIVPPLRSR
                     YLAEIAGEIRNYHTIVAEQAGLAREIQQLKSSRAMLEAGGASGEALGPLIAEREARVD
                     PRARKLIENWPKLKARYAGDDFVVKVRGREIRTRQVSVSLSGTPVRKVALPRYEDHGE
                     LLRWILKENVPGAFPYTAGVFVFKREGEDPQRMFAGEGDPFRTNARFKYLSKDSEAKR
                     LSTAFDSVTLYGWNPDERPDIYGKVGNSGVSIATLDDMKALYAGFDLCAPQTSVSMTI
                     NGPAPMLLAMFFATAIDQQLAKFAADRGRPPDAEETSGIRAATLETVRGTVQADILKE
                     DQGQNTCIFSTEFALKMMGDIQEYFVRHRVKNFYSVSISGYHIAEAGANPISQLAFTL
                     ANGFTFVESYLARGMKIDDFAQNLSFFFSNGMDPEYTVIGRVARRIWAVALRFKYGAG
                     ERSQKLKYHIQTSGRSLHAQEMDFNDIRTTLQALIAIYDNCNSLHTNAFDEAFTTPTE
                     NSVRRAMAIQMIINREWGLARNENSNQGSFIIEELTDLVEEAVLQEFERIAERGGVLG
                     AMETGYQRGRIQEESLLYEQKKHDGSLPIIGVNTFVNPHQAVMPPPPLQRSTMGEKDS
                     QIVRLRDFERAHAGESPAMRERLRAAVIGNENVFDVLMDAVRVCSLGQITDTLFEVGG
                     QYRRNM"
BASE COUNT          719 a         1229 c         1257 g          637 t
ORIGIN      
        1 catccaaggt gggagccgta tgcgggaaag ccgcacgtac ggttctgtgc ggggggcgcg
       61 tagcaatacg cgtccctacc gcgatccgta ttcatttgtg agaccccgga cctttttgtg
      121 aatcgttttg gcgccgggcc ccctccgtca cgcgccttcg gcgcgcgcca cctcccccac
      181 gcgttccgcg cgggggagga aaacgcggtt ttttcctccc ccgtgcgcag catgggggag
      241 ggggaccgcg aagcggtgga gggggcaagc ggcgccgaga gagccccctc tgccccttcg
      301 gggcacctcc cccatgcttc gcacggggga ggaaacgttc cgcgcggggg aggaatggat
      361 tatattggcg ccgaggagca ggctatgaac gacgtcacga ccgcacggcg agacaagatc
      421 gagcgccccc tgcctacgca ggggccgccg cacacgacac gcaacaagat ccgcttcgtc
      481 accgcgacca gcctgttcga cgggcacgac gcggccatca acatcatgcg gcggatcctc
      541 caggcgtccg gcgccgaagt gattcatctc ggtcacaacc gctcggtgga cgaggtggtc
      601 accgccgcga tccaggagga tgtgcagggc atcgccgtca gctcctatca gggcggccat
      661 gtcgaatact tcaaatatgc ggtcgacctg ctgcacgagc gcggcgccgg gcggatcaag
      721 attttcggcg gcggcggcgg cgtcatcacc agggaggaga tggacgagct acacgcctat
      781 ggcgtcgaac ggctctattc ccccgccgac ggccagcgcc tgagcctcca gggcatgatc
      841 gacgacatga tggcgcgcgc cgatttcgat ccggccgacg acggcgcggt ggaggttcgc
      901 acgctggcga cgcccggatg gcggccgctg gcgcgcttcc tcacccgcct ggaacacggc
      961 cgcgtcgatg cggcgacgct cacgaattta actggcaacc gcgacaaacg ccgcgccatg
     1021 cccgtcctcg gcatcaccgg caccggcgga tcgggcaagt cttcgctgtg cgatgagctg
     1081 atccgccgct tccgtctcga cagcgaggaa ttcaggatcg cggttctggc gatcgatccg
     1141 tcccggcgca agagcggtgg cgcgctgctg ggcgatcgca tccgcatgaa cgcgatcaat
     1201 gcgccgcaga tctacatgcg ctccatcgcg accagggatt cggagagcga agtgccgcag
     1261 ttcattcccg ccgccgtgga agcagcccgc gccgccggat tcgatctcgt catcgtggag
     1321 acgccgggca tcggccaggg caacaccgcc atcgccgatc ttgccgatat ttcgctctat
     1381 gtgatgacgc cggaattcgg cgcggcctcg caactcgaaa agatcgacat gctcgacttc
     1441 gcggatctgg tggcgatcaa caaattcgac cgccgcggcg ccgaagatgc gctgcgcgat
     1501 gtccgcaagc aggtccagcg caaccgccag ttcttcaacc ggccgccgga atcgatgccc
     1561 gtctatggcg ccatcgcggc gcggttccag gacgacggcg tcaccgggct ttatctcggc
     1621 ttgcgcgatc tcctggtgga gaagggcttc catccgccgc ccagccgctt cgatgcgaaa
     1681 agcgaccgcg ttccttcgcc caggcaggcc atcgtgccgc ccctgcgttc gcgctacctg
     1741 gccgaaatcg ccggcgaaat ccgcaactat cacacgatcg tcgcggagca ggcgggtctg
     1801 gcgcgcgaga ttcagcagct gaaatcctcg cgggccatgc tcgaagctgg cggggcgagc
     1861 ggcgaagcgc tggggccgct cattgccgaa cgcgaggcgc gcgtggatcc gcgggcgcgc
     1921 aagctgatcg agaactggcc caaactcaag gcgcgctatg cgggcgacga cttcgtggtg
     1981 aaggtgcgcg gccgggaaat ccgcacccgc caggtgagcg tcagcctgtc gggcacgccg
     2041 gtgcgcaagg tggcactgcc gcgctacgaa gatcacggcg agctgctgcg ctggatattg
     2101 aaggagaacg tgcccggcgc gtttccctat acggcgggcg tgtttgtctt caagcgcgaa
     2161 ggcgaggatc cccagcgcat gttcgcgggc gaaggcgatc cctttcgcac caatgcgcgc
     2221 ttcaaatatc tgtcgaagga ttcggaggcg aagcggctgt cgacggcctt cgattcggtg
     2281 acgctttacg gatggaaccc ggacgagcgg cccgacatct acggcaaggt cggcaattcg
     2341 ggcgtgtcga tcgcgacgct cgacgatatg aaggcgctct atgccggctt cgatctctgc
     2401 gcgccgcaga cctcggtgtc gatgacgatc aacgggccgg cgccgatgct gctggcgatg
     2461 ttcttcgcga ccgcgatcga ccagcagctg gcgaagtttg ccgccgaccg cggccggccg
     2521 ccggacgccg aagagacatc ggggattcgc gcggcgacgc tcgaaacggt ccgcggcacg
     2581 gtgcaggccg atatcctgaa ggaagaccag ggccagaaca cctgcatctt ctcgaccgag
     2641 ttcgcgctga agatgatggg cgacatccag gaatatttcg tccgccacag ggtgaagaat
     2701 ttctattcgg tgtcgatttc cggctatcac atcgccgaag cgggcgccaa tccgatctcc
     2761 cagctcgcct tcacgctcgc caacggcttc accttcgtgg agtcctatct ggcgcgcggc
     2821 atgaagatcg acgatttcgc gcagaacctg tcgttcttct tctccaatgg catggatccg
     2881 gaatacacgg tgatcggccg cgtggcgcgg cgcatctggg ccgtcgcgct gcgcttcaaa
     2941 tacggcgcgg gcgaacgctc ccagaagctg aaatatcaca ttcagacgtc cggccgctcg
     3001 ctgcacgccc aggagatgga tttcaacgac atccgcacga cgctgcaggc gctgatcgcg
     3061 atctacgaca attgcaacag cctgcacacc aacgccttcg acgaagcgtt cacgacgccg
     3121 acggagaatt cggttcgccg cgcgatggcc atccagatga tcatcaatcg cgaatggggg
     3181 ctggccagga acgagaattc caaccagggc tccttcatca tcgaggagct gaccgacctt
     3241 gtcgaagagg ccgtgctgca ggaattcgag cgcatcgccg aacgcggcgg cgtgctcggc
     3301 gccatggaga ccgggtatca gcgcggcagg atccaggagg agagcctcct ctacgagcag
     3361 aagaagcatg acggcagcct gccgatcatc ggcgtcaaca cgttcgtcaa tccgcaccag
     3421 gcggtgatgc cgccgccgcc gctgcagcgt tcgaccatgg gcgagaagga tagccagatc
     3481 gtgcggctgc gcgatttcga gcgggcgcac gcgggcgaat ctccggcgat gcgcgagagg
     3541 ctgcgggctg cggtgatcgg caacgagaac gtcttcgacg tgctgatgga cgcggtgcgc
     3601 gtctgttcgc tgggccagat caccgacacg ctgttcgaag tcggcggcca gtaccggcgg
     3661 aatatgtgac gtccgtacct ggaatcagtg acgagtgaca taaagacaga tttaacgcag
     3721 agggcgcgga ggatgcgcag tggacgcagg aaagagttgg cgcgccgcag gcgcgcgatt
     3781 cgtgccctgc gtcctccgcg catcctccgc gtactctgcg ttaaaaatct gccgacacac
     3841 ga
//
LOCUS       DASZMC010000348         5341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3702763,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000348 DASZMC010000000
VERSION     DASZMC010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3702763"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2536
                     /locus_tag="VG274_07100"
     CDS             <1..2536
                     /locus_tag="VG274_07100"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HWE06457.1"
                     /translation="ESNLDPLHLAPKVPHPQLEPSFYGFNDADVDRPVFVGGVLGLET
                     VTPRRMIDLLKRTYCGRIGYEFMHINDAEQKNWLQRRIEGPDKEISFTPEGKRAILNK
                     LVEAEGFEKFCAVRFVGTKRFGLDGGEAMIPALEQIIKRGGQLGVKEIVLGMSHRGRL
                     NVLANVMAKPYRQIFHEFQGGSTNPGEVEGSGDVKYHLGASSDREFDNRRVHLSLSAN
                     PSHLEAVDPVVLGKARAKQDQLNDEAGRASVLPLLIHGDAAFAGQGVVAECFAMSGTK
                     GFRTGGTIHFIVNNQIGFTTAPAYARSSPYPSDVALMVQAPIFHVNGDDPEAVVHCAR
                     IATEFRQLFHKDVVIDMFCYRRFGHNETDEPAFTQPSMYRAIREHPTTLALYAARLVA
                     DHTLVEGDVQTMLREFNGRLDVELNAAKSHKPERADWLDGRWTGMEVAPDGDRRGKTA
                     VPLETLKSIGRAFTSVPGGFHLHRTIARQFEAKAKLFETGQGIDWAMAEALAFGTLLA
                     EGCPVRLSGQDSSRGTFSQRHSVLVDQENETRYVPLAHVREGQAAFEVVDSLLSEQAV
                     LGFEYGFSSAEPRALVLWEAQFGDFANGAQVVIDQFIASGEMKWLRMCGLVLLLPHGY
                     EGQGPEHSSARLERFLQLCAQDNIQVANCTTPANYFHILRRQVHRPFRKPLVLMTPKS
                     LLRHKRVVSNLSDMAPGSGFHRILWDTAQAHPGSTLALVADGEIARVVLCSGKVYYDL
                     LEEREKRGEKRIQLLRIEQLYPFPQNALAAELARFPKAEIVWCQEEPKNQGAWTFVAP
                     RIHAAIRQPRGWPRYIGRPDYASTATGLLSQHTTELHAFLAEALTL"
     gene            2727..3977
                     /gene="odhB"
                     /locus_tag="VG274_07105"
     CDS             2727..3977
                     /gene="odhB"
                     /locus_tag="VG274_07105"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HWE06458.1"
                     /translation="MSIEIKVPTMGESVTEATVGRWFKKVGDAVARDEPLLELETDKV
                     TVEVPAPADGAIESIAVNQGATVGVGTVLGAIAEGTAAKSAASASVRNETPPTAPSPK
                     PASAPPIAQTAPQPVKAETSDDLMPSVRRIAEESGVRASDAAGTGKDGRVMKGDMLAA
                     LEARAAERAREPVAIAPAAQRPRADREERVPMSRLRRTIALRLKESQNTAAQLTTFNE
                     VDMSHVMALRTEYKESFEKKHHVRLGFMGFFVKACIAALKELPSVNGEIEGGDFIYKT
                     YYDIGVAVSTERGLIVPVVRDADRLSLAQIEAAIADFGTRAREGTLRLEELQGGTFTI
                     SNGGVFGSLMSTPILNAPQSGILGMHKIQPRPVAIDDRVEIRPMMYLALSYDHRMVDG
                     REAVTFLVRVKENLEDPQRLLLDV"
     gene            4164..>5341
                     /gene="lpdA"
                     /locus_tag="VG274_07110"
     CDS             4164..>5341
                     /gene="lpdA"
                     /locus_tag="VG274_07110"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWE06459.1"
                     /translation="MSDQFDVVVIGGGPGGYNAAIRAGQLGLTSACVDDRGTFGGTCL
                     NIGCIPSKALLYTSEMYEDAAKHFAPIGIKASVSLDLPAMMAHKTRVVGELTRGVEFL
                     FRKYKSEGIVGEARIAAPGKVDVKLRDGNTRTLAVKHIVIATGSDVAPLQGITIDEKR
                     IVSSTGALALDSVPKRLVVVGAGYIGLELGSVWRRLGSEVTVVEFLDRITPGLDHEIA
                     KQFQRILQRQGMKFLLSSKVSGVEARGEGLSVQVEGTNGEKQTLDADIVLVAIGRRPF
                     TDGLGLDELGVTRDPRGRVITDTEFRTNVEGVRAIGDCRDGPMLAHKAEDEAVACIER
                     IAGRAGHVGYDAIPAVVYTAPQVASVGKTEEELKAAGIAYKVGKFPFSANARAKTLAA
                     T"
BASE COUNT         1003 a         1745 c         1708 g          885 t
ORIGIN      
        1 cgaatccaat ctcgatcctc tgcatctcgc gccgaaggtg ccgcacccgc agcttgagcc
       61 gtcgttctat ggcttcaacg acgccgatgt ggatcgtccg gtgttcgtcg gcggcgtact
      121 cggcctcgaa acggtcacgc cgcgccggat gatcgatctg ctgaagcgca cctattgcgg
      181 gcgtatcggc tacgagttca tgcatatcaa cgatgcggag cagaagaact ggctgcagcg
      241 ccgcatcgag ggcccggaca aggaaatatc gttcacgccc gaaggcaagc gcgcgatcct
      301 gaacaaactc gtcgaagcgg aaggcttcga gaaattctgc gccgtccgtt tcgtcggcac
      361 caagcggttc gggctcgacg gcggcgaggc gatgatcccg gcgctcgagc agatcatcaa
      421 gcgcggcggt caactcgggg tgaaggagat cgtgctcggc atgtcgcatc gcgggcggtt
      481 gaacgtgctc gccaacgtga tggcgaaacc ctatcgccag atttttcacg agttccaggg
      541 tggcagcacc aatccgggcg aggtcgaagg gtcgggcgat gtgaaatacc accttggcgc
      601 ctcctcggac cgcgagttcg acaacaggcg cgtgcatctg tctctttcgg caaacccttc
      661 ccatctcgaa gcggtcgatc ccgtcgtcct cggcaaggcg cgcgcgaagc aggatcagct
      721 gaacgacgag gccggccgcg ccagcgtgct gccgctgctc attcacggcg acgcggcatt
      781 cgccggccag ggcgtggtgg cggagtgctt cgccatgtcg ggcaccaagg gcttccgcac
      841 cggcggcacg attcatttca tcgtcaacaa ccagatcggc ttcaccaccg cgccggccta
      901 tgcgcgctcc tcgccctacc cctccgacgt ggcgctcatg gtgcaggcgc cgatcttcca
      961 tgtgaacggc gacgatcccg aagcggtcgt gcattgcgcg cgcatcgcca ccgaattccg
     1021 ccagctgttt cacaaggatg tcgtcatcga catgttctgc taccggcgtt tcggccacaa
     1081 cgagaccgac gagccggcct tcacccagcc ttccatgtat cgcgcgatca gggagcatcc
     1141 aacgacgctg gcgctttatg cggcgcggct cgtcgcggat cacacgctcg tcgaaggcga
     1201 cgtccagacg atgctgcgcg aattcaatgg gcggctcgac gtggagctga acgcggcaaa
     1261 atcgcacaag cctgagcggg ccgactggct cgacgggcga tggacgggga tggaggtagc
     1321 accggatgga gaccgccgtg gcaaaaccgc cgtgccgctt gaaacgctga aatccatcgg
     1381 ccgcgcgttc acctcggtgc cgggtggttt tcatctgcac aggaccatcg cgcgccagtt
     1441 cgaagccaag gcgaagctgt tcgagaccgg ccagggaatc gactgggcga tggccgaggc
     1501 tctcgccttc ggcacgctgc tcgccgaagg ttgcccggtg cgcctctccg gccaggattc
     1561 ctcgcgcggc accttctcgc agcgccattc cgtgctcgtc gatcaggaaa atgagacgcg
     1621 ttacgtgccg ctcgcccatg tgcgcgaggg tcaggctgca ttcgaagtgg tcgattccct
     1681 cctctccgaa caggcggtac tcggcttcga atatggtttc agctcggcgg agccgcgcgc
     1741 gctcgtgctg tgggaagccc agttcggcga tttcgccaat ggcgcacagg tcgtcatcga
     1801 ccagttcatc gcttcgggcg agatgaaatg gctgcgcatg tgcggcctcg tcctgctgct
     1861 cccgcatggc tatgaaggcc aggggccgga gcattcctcg gcgcgcctcg aacgcttcct
     1921 gcaactctgc gcgcaggaca atatccaggt cgccaactgc accacgcccg ccaattattt
     1981 ccacatcctg cggcgccagg tgcaccggcc gttccgcaag ccgctggtgc tgatgacgcc
     2041 gaaatcactc ttgcgccaca agcgcgtcgt ctcgaatttg agcgacatgg cgcccggctc
     2101 ggggttccac cgcattctgt gggacacagc acaagcgcat cccggctcga cgctcgcgct
     2161 cgtagccgac ggcgaaatcg cgcgtgtcgt gctgtgctcg ggcaaagtct attacgacct
     2221 tctggaggaa cgcgagaagc gcggcgagaa acgcattcaa ttgctgcgca tcgagcagct
     2281 ctatccgttc ccgcagaatg cattggcggc cgagctcgcg cgtttcccga aagcggagat
     2341 cgtgtggtgc caggaggagc cgaagaacca gggcgcgtgg acgtttgttg cgccgcgcat
     2401 ccatgcggca atccggcagc cgcgcggatg gccgcgctac atcggccgcc ccgattacgc
     2461 ctccaccgcc accggcctct tgagccagca caccaccgaa ctccacgcct tcctcgccga
     2521 ggcgctgacg ctatgagcgc cgtgagcccc ctccgcccgc ttcgcgggca cctcccccaa
     2581 cgcttagcgt tgggggagga aaagcgcgtg cgtgccgaac ccgcgctcat cttttcctcc
     2641 cccgcgcgaa gcgcggggga ggtgccgagc gcagcgaggc ggagggggcg cggcggcaaa
     2701 gacacactca acccaaggcg tcatcgatga gcatcgaaat caaggttccc acgatgggcg
     2761 aatccgtcac ggaagcgacc gtcgggcgct ggttcaagaa agtcggcgac gcggttgccc
     2821 gcgacgagcc gctgctcgaa ctcgagaccg acaaggtgac cgtcgaagtg cccgcgccgg
     2881 cggatggcgc gatcgaatcc atcgcagtca accagggggc aacggtcggc gtgggaaccg
     2941 tgcttggcgc gatcgccgaa ggcactgcag cgaagagcgc cgcgtctgcg tccgtgcgca
     3001 acgaaacgcc tccgactgcg ccgtcgccga aaccggcgtc tgcaccacca atcgcacaga
     3061 ctgcgccaca accggtgaag gcagaaacat ccgatgatct gatgccgtcc gtgcggcgca
     3121 tcgcggagga gagcggcgtg cgtgcctccg atgcagcggg caccggcaag gacggccgcg
     3181 tgatgaaagg tgacatgctc gcggcgctcg aagcgcgcgc cgccgagcgc gcgcgcgagc
     3241 ccgttgcgat tgcgcccgcc gcgcaaaggc cgcgcgccga ccgcgaggag cgcgtgccga
     3301 tgtcgcggct gcggcgcacc atcgcgctcc gcctcaagga atcgcagaac accgccgccc
     3361 agctcaccac cttcaacgaa gtggacatga gccacgtgat ggcgctgcgc accgagtaca
     3421 aggagagctt cgagaagaag catcatgtgc ggctcggttt catgggcttc ttcgtcaagg
     3481 cctgcatcgc cgcactcaag gaactgccga gcgtcaatgg cgagatcgaa ggcggcgact
     3541 tcatctacaa gacctattac gacatcggcg tcgcggtctc gaccgagcgc gggttgatcg
     3601 ttcccgtggt gcgcgacgcg gaccggctga gcctcgcgca gatcgaagcc gcgatcgcgg
     3661 atttcggaac gcgcgcccgc gagggcacgc tccggctgga ggagctgcag ggcggcacct
     3721 tcaccatctc gaatggcggc gtcttcggct cgctgatgtc gacgccgatt ctcaacgcgc
     3781 cccaatcggg cattcttggc atgcacaaga tccagccgcg gcccgtcgcc atcgacgacc
     3841 gcgtcgagat ccgcccgatg atgtatctcg cgctctccta cgatcaccgc atggtggacg
     3901 gccgcgaagc ggtcaccttt ctcgtgcgcg tgaaggaaaa cctcgaagac ccgcagagat
     3961 tgctgctcga tgtgtagcgc gaattacctt caccaaaaac cagacatgtc atccccgcga
     4021 aagcggggac ccaggggcaa ccgcacatcg cttgctctgg attcctcccc gcgaaagcgg
     4081 ggatcaggcg ggaatgacat tcattgggtt ttgatttcat cgggcaatga cgtgaagcca
     4141 acaatcaaag gcgagatcgt ctaatgtccg atcaattcga tgtcgttgtt atcggcggcg
     4201 gaccgggcgg ctacaatgct gcgatccgcg cgggccagct cgggcttaca tccgcctgcg
     4261 tcgacgaccg cggcacgttc ggtggcacct gtctcaacat cggctgcatc ccgtcgaaag
     4321 cgctgctcta tacgagcgag atgtacgagg acgcggcgaa acattttgcg cctatcggca
     4381 tcaaggcgag cgtgtcgctc gatctccccg cgatgatggc gcacaagacg agggtggtgg
     4441 gcgagctgac gcgcggcgtc gagttcctgt tccgcaaata caaatccgaa ggaatcgtcg
     4501 gcgaggcgcg catcgcggcg cccggaaagg ttgatgtgaa actgcgcgac ggcaatacgc
     4561 gaacgctcgc cgtcaaacac atcgtcatcg ccaccggttc cgacgtggcc ccgcttcaag
     4621 gcatcacgat cgacgagaaa cgcatcgtct cctccaccgg cgcgctcgcg ctcgacagcg
     4681 tgccgaagcg gctcgtcgtg gtgggcgctg gttatatcgg cctcgagctg ggctcggtgt
     4741 ggcgcaggct cggcagcgag gtgacggtgg tcgaatttct cgaccgcatc acgcccggcc
     4801 tcgaccacga gatcgccaaa cagttccagc gcatcctgca gcgccagggc atgaagttcc
     4861 tgctgtcgag caaagtgtca ggcgtcgaag cgcgcggcga aggattgagc gtgcaggtgg
     4921 aaggcacgaa cggggagaag cagacgctcg acgccgatat cgtgctcgtc gccatcggcc
     4981 gccggccgtt caccgatggg ctcggcctcg acgagctggg cgtgacgcgc gatccgcgcg
     5041 gccgtgtgat caccgacacg gaattccgca ccaatgtcga gggcgtccgc gccatcggcg
     5101 attgccgcga cgggccgatg ctcgcccaca aggcggaaga cgaggccgtc gcctgcatcg
     5161 agcgcatcgc cggccgcgcc ggccatgtcg gctacgacgc aattcccgcg gtcgtctaca
     5221 ccgcgccgca ggtcgcgagc gtcggcaaga ccgaagaaga actgaaggcg gcgggcatcg
     5281 cgtacaaagt gggcaagttt ccctttagcg ccaatgcgcg cgccaagact ctcgccgcga
     5341 c
//
LOCUS       DASZMC010000349         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3164518,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000349 DASZMC010000000
VERSION     DASZMC010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3164518"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VG274_07115"
     CDS             <1..956
                     /locus_tag="VG274_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWE06460.1"
                     /translation="WEKEDAMREGVAILNYHVPKAFLHDNGKLRGMTFEKVAASRDEN
                     GRRILSPTGEPDVHIPCDDVLIAVGQENAFPWIEPEAGVEFDSVGLPKLDPVTFQATL
                     PSVFFGGDSAFGPKNIITAVAHGHEAAISIDAYCRGEDVRRRPPPMTNLVSQKMGIHE
                     WSYDNAITLDKRYKVPHDRRPAALKNIRAEVELGFDPALAVSEAQRCLNCDVQTAFTA
                     QACIECDACMDICPEDCISFVPDAEDESELRRSLIAPSPNPSQAIYVSAPLKTGRIMA
                     KDEDLCLHCGLCAERCPTGAWDMQKFTLDMAQAGSACQSRA"
     gene            938..>2275
                     /locus_tag="VG274_07120"
     CDS             938..>2275
                     /locus_tag="VG274_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWE06461.1"
                     /translation="MPIQGVNDFVIRFANVNGSGSASANLLFARSVLRMGAPIAPRNI
                     FPSNIQGLPTWFEVRVSAEGHLGARGGVDMMVAMNPQTFARDVESIAPGGYLFYDSSR
                     PIAISRMRSDITILGMPLTDICAREYAEPRQRQLFKNIVYVGALAALLDIELAEVEKL
                     IGEQFRGKDRLIGPNLRALKIGYDDAKARFICPLGMRVARSGAAGDRILVEGNFAAAL
                     GAVYGGATVCAWYPITPSTSLAEAFDKHGSKFRVDRATGERRYAIVQAEDEIAAIGMV
                     VGAGWNGARAFTATSGPGVSLMQEFLGLAYFAEIPAVIFDVQRGGPSTGMPTRTQQSD
                     LLACALASHGDTKHVLIFPQDPGEAFEFGALAFDLADQLQTPIFVMLDLDIGMNHHLC
                     KPFTWDDSRTFDRGKVMTAEELESGKTFGRYLDVDGDAIPYRTLPGTHPSKGAY"
BASE COUNT          414 a          762 c          680 g          419 t
ORIGIN      
        1 cctgggagaa ggaggacgcg atgcgggaag gcgtcgcgat cctgaactac catgtcccca
       61 aggcttttct tcacgacaac ggaaagctga ggggcatgac cttcgaaaag gtcgccgcct
      121 ccagggatga aaacggccgc cgcattctta gccccaccgg cgagccggac gttcatatcc
      181 cctgcgacga tgtgctgatc gcggtcggac aggaaaacgc ctttccctgg atcgaacccg
      241 aggccggcgt ggaattcgac agcgtgggcc tgccgaagct cgaccctgtc acttttcaag
      301 caacgctgcc ctcggtcttc ttcggcggcg attccgcctt tggtccgaag aacatcatca
      361 ccgccgtcgc gcacggccat gaggccgcta tctcgatcga cgcctattgc cgcggcgagg
      421 atgtccgccg ccgcccgccg cccatgacca atctcgtcag ccagaagatg ggcattcacg
      481 aatggtctta cgacaatgcg atcacgctcg ataaaaggta caaggttccg cacgaccgcc
      541 ggccggctgc gctgaagaac atccgcgcgg aggtcgagct cggcttcgat ccggcgctcg
      601 ccgtttcgga ggcgcagcgc tgtctcaatt gcgacgtgca gacggctttc accgcgcagg
      661 cctgcatcga atgcgacgcc tgcatggata tttgtcccga ggactgcatc tccttcgtcc
      721 ccgacgcgga agatgaaagc gaattgcgcc gctctttgat cgcgccctcc cccaatccat
      781 cacaggcgat ctacgtctcg gcgccgctga agaccggacg catcatggcg aaggacgagg
      841 atctctgtct gcactgtggt ctgtgcgccg agcgttgccc caccggcgcg tgggacatgc
      901 agaaattcac gctcgatatg gctcaggcgg ggagcgcatg ccaatccagg gcgtaaacga
      961 cttcgtcatc cgcttcgcca atgtcaacgg ctccggctcg gcgagcgcca atctcctgtt
     1021 cgcccggtcg gtcctgcgca tgggcgcgcc gatcgcgccg cgcaacatct ttccttcgaa
     1081 cattcaaggc ctgccgacct ggttcgaagt gcgcgtctcc gcagaaggcc atctcggcgc
     1141 ccgcggcggc gtcgacatga tggtggcgat gaatccgcaa actttcgcca gagatgtcga
     1201 atcgatcgcg cctggcggct atcttttcta tgattcctcg cgcccaatcg ccatctcgcg
     1261 catgcgctcg gacataacga tcctcggcat gccgctgacc gacatctgcg cgcgcgaata
     1321 tgccgagccg cgtcagcgcc agctcttcaa gaacatcgtc tatgtcggcg cgctggcggc
     1381 cttgcttgat atcgagctcg ccgaggtgga gaagctgatc ggcgagcagt tcaggggcaa
     1441 ggaccgcctg atcggaccga atctgcgcgc gctgaagatc ggctatgacg acgccaaggc
     1501 tcgcttcatc tgtccgctcg gcatgcgcgt cgcgcgcagc ggcgcagcgg gcgatcgcat
     1561 tctcgtcgaa ggcaatttcg cggcggcgct gggcgcggtc tatggcggcg ccaccgtctg
     1621 cgcctggtat ccgatcacgc cctcgacttc gctcgctgaa gctttcgaca aacatgggtc
     1681 caaattccgc gtcgatcgcg ccaccggcga gaggcgctac gccatagtcc aggcggaaga
     1741 cgagatcgcc gctatcggca tggtggtcgg cgccggctgg aacggcgcgc gcgccttcac
     1801 ggcgacttcc gggcccggcg tatcgctgat gcaggagttt ttgggcctcg cctatttcgc
     1861 cgaaatcccc gccgtcatat tcgacgtgca gcgcggcggc ccctcaacgg gcatgccgac
     1921 gcgaacgcag caatccgatc tgctcgcctg cgcgctcgct tcacatggcg acaccaaaca
     1981 tgtgctgatc tttccgcagg accccgggga ggcgttcgaa ttcggcgcgc tggctttcga
     2041 tctcgccgat cagttgcaga cgccgatctt cgtcatgctc gacctcgaca tcggcatgaa
     2101 ccaccatctc tgcaagccgt tcacgtggga cgacagccgc acgttcgacc gcggcaaggt
     2161 gatgacagcc gaggaactgg aatccggcaa gaccttcggc cgctacctgg atgtggacgg
     2221 cgacgccatc ccctaccgca ccctgcccgg cacccatccc agcaagggcg cctat
//
LOCUS       DASZMC010000350         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4539373,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000350 DASZMC010000000
VERSION     DASZMC010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4539373"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VG274_07125"
     CDS             <1..1464
                     /locus_tag="VG274_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HWE06462.1"
                     /translation="PDAPPLVVAAKEKNALVIAALDRKATALGLSVGQPLANARAMLP
                     ALKAVTANEPADVKLLGRIADWCERFTPFVSLDPPRGLLLDVTGATHLFGGEQAMLDR
                     LLGGLATQGFAVRGAMAGTVLAARALARFRDGAVVAPGEEEKAITSLPIDALNLDPVT
                     TYAFRRAGLKTIGQAAGRKRSELNARFGARLVYMIDSALGRAESPISPRRPLPDYRAE
                     RNFAEPVATEEVIRSTLVSLAASLGEILEKRGEGARRLEAEFYRADGQVRRIAVETGS
                     PIRDPKIVERLFREKLDALIDPLDPGFGFDLIRLGATRAERAETAAADFDADLNAKRE
                     IRFLVDRLAARFGSQRILAFQPNDTHIPECAFATVPAQYAEETKVTWEKIRRPGEAPR
                     RPLRLLAKPEPMSLLRAVPGSNAAHMRWRRAQRTVIHAEGPERIAMEWWRHQDPQPTR
                     DYHRIEDADGRRVWIFRDGIPDREDFSAQWRVHGAFA"
     gene            1595..>1806
                     /locus_tag="VG274_07130"
     CDS             1595..>1806
                     /locus_tag="VG274_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWE06463.1"
                     /translation="MITSPFAEIGVTTNFSFLRGASHPDEMAHQATLLGYHAIGIADR
                     NTLAGIVRMYHALKQLREKTGFAPKLL"
BASE COUNT          319 a          621 c          557 g          309 t
ORIGIN      
        1 cctgacgcgc cgccgctggt cgtcgccgcg aaggagaaga acgcgctcgt catcgccgcc
       61 ctcgaccgca aggcgacagc gcttggcctg agcgtcggtc agccgctcgc caatgcgcgc
      121 gcgatgctgc ccgcgctgaa agccgtgacg gcgaacgagc ccgccgatgt gaaattgctc
      181 ggccgcatcg ccgattggtg cgagcggttc acaccattcg tatcgctcga tccgccacgc
      241 ggcttgttac tcgacgtcac cggcgccacg catctcttcg gcggcgaaca ggcgatgctg
      301 gacaggttgc ttggaggcct tgccacacaa ggtttcgcgg tgcgcggcgc gatggctggc
      361 acggtactgg ccgcacgcgc cctggcgcga tttcgcgatg gcgctgtcgt ggcccccggc
      421 gaagaagaaa aagcaatcac gtcgctgccc atcgacgcgc tcaatctcga tcccgtgacg
      481 acgtatgcgt ttcgccgcgc aggcctgaag acgatagggc aggcggcggg ccgcaagcgt
      541 agcgaactca atgcccgctt cggtgcgcgg ctggtctata tgattgatag cgcactcggg
      601 cgggccgaaa gcccaatctc gccgcgccgt ccgctgcccg attaccgcgc cgaacgcaat
      661 ttcgccgagc cggtcgcaac cgaagaggtc atccgttcta ctctggtatc gttggccgca
      721 tcactaggcg aaattttgga gaaacgcggc gaaggcgcac ggcgtctgga agcggaattc
      781 taccgcgccg acggacaggt gcgccgcatc gcggtcgaga ccggctctcc catccgcgat
      841 cccaagatcg tcgagcgcct gttccgcgag aagctcgatg cgctgatcga cccgctcgat
      901 cccggcttcg gcttcgatct catccgcctc ggcgcgacgc gcgccgaacg cgcggaaacc
      961 gccgccgccg atttcgatgc cgacctgaac gcgaagcgcg agatccgttt cctggtcgac
     1021 cgcctcgccg cccgtttcgg atcgcaacgc atcctcgcct tccagcccaa cgacacgcac
     1081 atccccgaat gcgccttcgc cacagtcccg gcgcaatatg cggaagagac aaaggtcact
     1141 tgggaaaaaa tccgccgccc gggtgaggcg ccgcgaaggc cgctacgtct gctcgccaag
     1201 cccgagccca tgagtcttct gcgcgccgtg ccgggaagca atgccgctca catgcgctgg
     1261 cgccgggcac aacgcaccgt catccatgcc gagggtcccg aacgcatcgc gatggaatgg
     1321 tggcgccatc aggacccgca gccgacgcgc gattatcacc gcatcgagga cgcagacggc
     1381 cgccgtgtct ggattttccg cgacggcatc cccgatcgtg aggatttctc cgcgcaatgg
     1441 cgcgtgcacg gggccttcgc gtgagcgctc gctttcttaa cctccccctt gtggggaggt
     1501 cggcgaacgt agtgagccgg gtgggggtcc gctcccgcca ccgacccccg cccgaagcgc
     1561 tttgcgcttc gacctcccca caagggggag gttagtgatc acgtccccct tcgccgaaat
     1621 cggagtcacg acgaatttct ccttcctgcg cggggcgtcg catccggatg agatggctca
     1681 tcaggcaaca cttctcgggt atcacgctat cgggatcgcg gaccgcaata cgttagcagg
     1741 catcgttcgc atgtatcatg cgctcaagca gcttcgcgag aagactggct ttgcaccgaa
     1801 gcttct
//
LOCUS       DASZMC010000351         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5093281,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000351 DASZMC010000000
VERSION     DASZMC010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5093281"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VG274_07135"
     CDS             <1..378
                     /locus_tag="VG274_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06464.1"
                     /translation="YLASLSHMKDGRNLAERLLKQNPGNYDVYLAIGVENYILSLKPA
                     PVRWLLHMGGAQTDKAIGLQNLATTAEKGRYLLPFARLLLAVAALRDGDVTAARERLA
                     WLAAQFPDNRLYREELAKLPAGH"
     gene            469..1539
                     /locus_tag="VG274_07140"
     CDS             469..1539
                     /locus_tag="VG274_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HWE06465.1"
                     /translation="MKILAAASAFPEHYCPQADATAALIETWGDRLSSPDALRRIHSR
                     AQVDGRYLALPLEAYPALKNWGEANKVWMRVAEKIGSDALCRAATKAGISSHDIDALF
                     FVSITGVSSPSMDAKIINRLHLNPHMRRVPIFGLGCVAGAAGLSQAADYVRAYPERIA
                     ALVSVELCTLTMQYDDLSMANLISTGLFGDGAAAVLVAGAERALPGVEHGPSGPEIVA
                     TRSVFYPDTEDVMGWDVSERGFRIVLSPGVPEIIGQHLRRDMDAFLADHDLTRSDIGC
                     WVMHTGGPKVLQATQEALEVDREALAASWDCLRRMGNLSSASVLIVLEEIMTRRRPAP
                     GTWSVLAAMGPGFCCEMLLLRW"
     gene            1563..>2438
                     /locus_tag="VG274_07145"
     CDS             1563..>2438
                     /locus_tag="VG274_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWE06466.1"
                     /translation="MFDTDVFVIGGGPAGLAAAIAAARQGLRVTVADGRRPPLDKACG
                     EGLLPDSLAAAADLGIMLPALETQPFCGIRFVRGDAHAEAAFRGVSSLKFSALGVRRT
                     VLHRVLTEHAERAGVTFLWGTSVSGIEGQAVRMPNTSISAKWIVGADGVCSRVRKWVS
                     LDACTRNHVRFGFRRHYAIAPWSEFMEVHWAAGAQVYVTPVADDEICVASLSRDPRVR
                     LDNALASLPDLRERLAGVAARSLERGAATVTRRLRAVVRGHIALIGDASGSVDAVTGE
                     GLGLLLRQALALGEAL"
BASE COUNT          410 a          714 c          793 g          521 t
ORIGIN      
        1 tatcttgctt cgctctccca catgaaggac gggcgtaacc ttgccgagcg actgctgaaa
       61 caaaatccag gcaactatga tgtctatctg gccatcggtg tggagaatta catcctgagt
      121 ttgaagcccg cgcccgtgcg ctggttgctg catatgggcg gcgcgcaaac ggataaggcc
      181 atcggcctgc agaatctcgc tacgaccgcc gagaaaggac gctatctctt gcccttcgcg
      241 cgattgcttc tggcggtggc ggcattacgc gatggcgatg tcacggcggc gcgcgaacgg
      301 ctcgcgtggc tggctgctca gtttcccgac aatcgcttat atcgggagga gttggcgaag
      361 cttcctgctg gccattagcg tgctgcttgg atccacggat gatgagaatc gcagctgtct
      421 gaaatcgtgg ccctatgaaa gtcgcggctt gatgaaaatt tcagctttat gaagatccta
      481 gcggcggcca gcgccttccc cgaacactat tgcccccagg cggacgcaac tgcagcgctg
      541 atcgagacct ggggcgaccg gctttcttcg cctgacgcac tgcgtcgcat tcattcacgc
      601 gcgcaggtgg acggtcgcta cctcgcgctg ccactggagg cctaccccgc gttgaaaaat
      661 tggggcgaag cgaacaaggt gtggatgcgc gttgccgaaa agatcggcag cgatgcgctc
      721 tgccgcgcgg ccacaaaggc cggcatctca tcgcacgaca tcgatgcact cttctttgtt
      781 tccatcaccg gcgtgtccag cccttcgatg gacgccaaga ttatcaaccg gcttcatttg
      841 aatccacata tgcgccgggt gccgattttt ggcctgggct gcgtggcggg tgcggccggc
      901 ctttcgcaag cagcggacta cgttcgcgcc tatcctgagc gcatcgccgc actggtttcg
      961 gtggagcttt gcacactcac catgcagtat gacgatttgt cgatggcaaa tttaatctcg
     1021 acgggcctgt ttggcgacgg cgccgcggcg gtgctggtgg cgggcgcgga acgggcgctg
     1081 cccggcgtgg agcatgggcc atccggtccc gaaatcgtgg ccacccgttc cgtgttttac
     1141 cccgacaccg aagacgtgat gggatgggat gtctccgaac gcggtttccg cattgtactg
     1201 tcgcccggcg tgcccgagat catcggccag catttgcgcc gggacatgga cgcgtttttg
     1261 gccgatcacg atcttacgcg ctccgacatc ggatgctggg tgatgcacac cggcggcccg
     1321 aaagtactgc aggcgacgca agaggctctc gaagtcgatc gcgaggcact tgccgcgtcg
     1381 tgggactgcc tgcgccgcat gggaaatctt tcctcggcgt ctgtgttgat cgtgctcgaa
     1441 gaaatcatga cgcgtcgccg gcccgcgccc ggcacgtgga gcgtgctggc ggcgatgggt
     1501 cccggctttt gttgcgagat gcttttgctg cgatggtagg cggcgcgaag gtcgcttaat
     1561 aaatgtttga caccgacgtg tttgtgatcg gaggagggcc ggccggcctt gccgctgcga
     1621 tcgcagccgc gcgccaggga ctgcgtgtca ccgtcgccga tggccgccgc ccgcctctcg
     1681 ataaggcttg cggcgaagga ttgctgccgg attctctcgc ggcggccgca gacctgggca
     1741 ttatgctgcc tgcgctcgag acacagccct tctgcggcat tcgcttcgtg cgaggagacg
     1801 cccacgcgga agcggcgttc cgcggtgtgt catctttaaa attctcagca cttggtgttc
     1861 gccggacagt tctgcatcgc gtgttgaccg agcatgcgga acgcgcgggc gttacatttt
     1921 tgtggggaac ttcggtgtca ggaatcgaag gccaagccgt gcgcatgccg aacacaagca
     1981 tctctgcaaa gtggatcgtc ggcgccgatg gcgtttgctc gcgcgtgcgg aagtgggtga
     2041 gcttggatgc ttgcacccgc aatcacgtcc gtttcggatt tcgccgccat tatgccatcg
     2101 ctccctggtc ggagttcatg gaagtgcatt gggcggcggg agcgcaagtg tacgtgacac
     2161 ccgtggccga cgacgagatc tgtgtggctt cgctttctcg cgatccgcgt gtgcgcctgg
     2221 ataatgcgct ggcttcgctg cccgatttac gcgagcgact cgccggagtt gcagcccgat
     2281 ccttggaacg cggcgccgca acggttacgc gacgtttgcg cgcggtggtg cgcggacata
     2341 tcgcgctgat cggcgatgcc tccgggtcgg tagatgcggt taccggagaa ggacttgggt
     2401 tgctgctccg gcaggcgctc gcactgggag aggcgctg
//
LOCUS       DASZMC010000352        12112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5372073,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000352 DASZMC010000000
VERSION     DASZMC010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5372073"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..800)
                     /locus_tag="VG274_07150"
     CDS             complement(183..800)
                     /locus_tag="VG274_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWE06467.1"
                     /translation="MEPATTLNAPVEPLASRRKPDSLAKLKRAGRRLFVERGYHATRP
                     QDIAREAGLGHGTFYLHFADKRACFLDFVEDARQELDSFLRQRVAAHETLEELIAGTL
                     HAIFEYSETHPGVLNAAMTDELVIDAEGAQAVPLLQRWGADWAETIRAAVKQNRIADI
                     YDADIVGQAILGAIHQTAVEGARAGRSRQAVVRNLTRFLVRALSA"
     gene            complement(1012..3375)
                     /locus_tag="VG274_07155"
     CDS             complement(1012..3375)
                     /locus_tag="VG274_07155"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWE06468.1"
                     /translation="MRFGIGQSVRRTEDMRFVTGYGQYTDDLQFPGEAFACFVRSPHA
                     HARLGALDVEAARVAAGVVGVLTQEDVETFGAGPMPCMAPVVSRDGTATKNSPKPLLA
                     KDRVTFAGEAVAMVVAETYAQAKDAAELVMVEYEPLDAGGTLDAAPASVQIWDDAPGN
                     LVFDWAAGNEKACGEAFARAVHVAAIEIVQNRVCANPMETRNAIGLYDIERDRYTLYT
                     GSQGAGGVRDRVAGVLGIQAQKLRALTPDVGGGFGMKGFIYPEQPLVLIAAKVLGRPV
                     RWSAERVESFLADAHGRDMRMRAELALDADGGILAMRIGGEANMGGYLPQFAPFIPTL
                     AGGRIFGGVYRVPHLHAEIKGYFSNAAPVDAYRGAGRPEAAYLMERLMDKAASVTGLD
                     RIEIRRRNLPAPEELPFRNWFGLEFDSGNFPLMLDEAADVADVPGFEQRRRKSEERDR
                     RRGLGIAYYVEITAATGGEPASVNFTDNGVVEAYVATQSSGQGHETAFAQIVAERLGV
                     PFESVIVKQGDSDWASGFGTGGSRSLNMAGGALEIASDEAIRKGKAAAAHVLQAGGAS
                     IGFKISEGVGTFRVEGTLRAITMPELALTLKRETIVGFEEGLDSNGQYRGTAPTFPNG
                     CHICEVEIDPETGVVEVLSYHVLDDFGRVINPMLVAGQVHGGVAQGLGQALIENCVYD
                     AETGQILTASFTDYALPRATDIPDIRFAYREFPCKTHAIGAKGCGEAGTVGALPAVMS
                     AVADAIGIDHLDMPATPERVWRALRKAPRSGCSVLPIESQTNCHGPA"
     gene            complement(3430..4929)
                     /gene="glpK"
                     /locus_tag="VG274_07160"
     CDS             complement(3430..4929)
                     /gene="glpK"
                     /locus_tag="VG274_07160"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008305563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HWE06469.1"
                     /translation="MDAEPPFLLAIDQGTTSTRAILFDASLRPLASHAIEVRQIYPAD
                     GWVEQDPEEIWQAVIACCRVAAKGVSPAEIAAIGLTNQRETTVLWERSTGKALHPAIV
                     WQDRRTTDLCRILKENGREPLVTARTGLVIDPYFSATKLQWLLNHVPNARGRAGELAF
                     GTIDSWLIFRLTGGRVHATDVTNASRTMLLDLRTLDWCDELLALFDVPRVVLPEVRDS
                     AGDFGNATEDLLGARIPICGVAGDQQAAMFGQACFAPGDVKATYGTGCFALVNTGIAV
                     PASRNRLLATAAYRAWGTTAYAIEGSIFVAGSVVQWLRDGLGVIESASEIEALIRRAR
                     GCEGLYFVPAFTGLGAPYWDPQARGAIVGLTRDAGAPEIARAALDAVCFQTRDLLAAM
                     NADMELAGFGRPEILKVDGGMVRNDAFCQRLADLTGLPVARPALTETTALGAASLAGL
                     GAGLFRDFADVASAWSLDRRFEPKLAGQTRDALYEGWCDAVKRVRSREN"
     gene            5076..6062
                     /locus_tag="VG274_07165"
     CDS             5076..6062
                     /locus_tag="VG274_07165"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HWE06470.1"
                     /translation="MSALAANSRKQSAIWLSVATLFSNQAPCLHTRERSLMRDILRRL
                     AGTADAAQRIVLAERIADDPAVPLELVLHLVDDSIEIAVPLILRSAALDEENLLALIA
                     RADVTRKILCAQRARIGERVAAALAKSDEECVLLALVHNSTAQISTTIFEILAEKSRR
                     FESLREPLARRSDLPAILWTKIREGLPEATKSLSEKGPVVPRPNDSPEASDNARKLID
                     KLAVAGQLGAGFLMRVLLQGQTELFDIAFARLLDLGLEEFRAAFYASDPQGMALACRA
                     VGIDRSVFPTVWGLSTKRRAGPRLFGSGRPAALDSLFALPKPEARARLHACS"
     gene            6108..7094
                     /gene="moaA"
                     /locus_tag="VG274_07170"
     CDS             6108..7094
                     /gene="moaA"
                     /locus_tag="VG274_07170"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWE06471.1"
                     /translation="MIDPFGRHITYLRVSVTDRCDFRCVYCMPEHMDFLPRAELLTLE
                     ELERLCRAFMRRGVERIRLTGGEPLVRRDIMRLIGRLGAAVGRDLRELTMTTNGSQLA
                     RFAKDLRAAGMRRVNVSLDTRDADKFRTITRRGDLSKVLAGIDAAQSAGLEIKINMVA
                     LKDVNEDEIESMLEWAHGRGMGLTLIETMPLGEIDGDRVGQYLPLSIVRARLAQRYTL
                     EESEFRTGGPARYVRVGETGGRLGFITPLTHNFCESCNRVRLTCTGTLYMCLGQADSA
                     DLRSPLRSSEDDALLDRAIDAAIARKPKGHDFIIDRRHQTPALARPMSVTGG"
     gene            7099..7863
                     /locus_tag="VG274_07175"
     CDS             7099..7863
                     /locus_tag="VG274_07175"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HWE06472.1"
                     /translation="MPRSLHFTASDAAEAQEALRRMRQSYGDAGADHAEIVIALGGDG
                     FMLQTLHAFLDRNRPIYGMNFGSIGFLMNEYGEEQLHERLDAAESARIHPLRMHAATR
                     AGKKVEALAFNEVSLLRETRQAAKIRILVDDKPRIAELICDGVLISTPAGSTAYNLSA
                     HGPILPIDADLLALTPISAFRPRRWHGALLSHRARTRFEMLEADKRPVSAVADDLEVR
                     DVRSVDVAEDRSITMTMLFDQGHNLDERILAEQFVD"
     gene            complement(7894..8691)
                     /locus_tag="VG274_07180"
     CDS             complement(7894..8691)
                     /locus_tag="VG274_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06473.1"
                     /translation="MSDQKSRKEVVPVPAATMLLARDGGDGIEVFMVKRHHEMGFAAG
                     ALVFPGGKACREDSDASLAGRMGGASDWSPPMRAVAVAAIREAFEEAGVLLARDCRTG
                     KIVAGERLAALDPYREKIEIGQTSLGEMLRIERLTLACDELVHFAHWITPKSMPKRFD
                     THFFLARAPTDHTGRHDGRESVDSLWARPEQAIAMRRQWNIMFPTKLNLMKLAKTGTV
                     AEALAAARATPPQTVEPWLEDGPDGKYFRIRDDAGYETTRIAVGDER"
     gene            complement(8799..9197)
                     /locus_tag="VG274_07185"
     CDS             complement(8799..9197)
                     /locus_tag="VG274_07185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HWE06474.1"
                     /translation="MFQLHECLAAESCLVGDWTLSRLLLAGDARFPWLILVPRRTGIS
                     EIHELDSSDRSILIEEIARASIGLRKMTKATRINIGALGNVVPQLHVHIVARHPGDAA
                     WPGPIWGHGAPLRYESSVRDDLIENLRRKI"
     gene            complement(9219..10340)
                     /gene="thiO"
                     /locus_tag="VG274_07190"
     CDS             complement(9219..10340)
                     /gene="thiO"
                     /locus_tag="VG274_07190"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HWE06475.1"
                     /translation="MKVVVIGGGVAGLGIGWRLAQRGVEVVLLERAQPGRGATWASAG
                     LITAKEDKAEGAGREAGFAQRSARLWQDFATEIERESGRTIAYRVDGRLVVAQTAEMY
                     AALAAQAELGAGQMLEAEAAREIEPRLAADLAGALWDRHDARVDNRALGPALAACFVK
                     SGGSLLLNETAVCFEIEGERIIGIRTPFALHQADAYVVAAGAWSAGIEGLPREAVPPV
                     FPVKGEMLALQPPAGETLPRISIGGQDAYLVAQRDRLLVGATVSRAGFDTGLTEGAAR
                     WLIDRAVALMPSLRDWPVVEHWAGLRPGSPDDLPLLGRSAIDGLFIASGQYRNGILFA
                     PAVADAMRAIVLGETLPPDIGAFDPRRFGASAAREQAPG"
     gene            10469..10951
                     /gene="msrA"
                     /locus_tag="VG274_07195"
     CDS             10469..10951
                     /gene="msrA"
                     /locus_tag="VG274_07195"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWE06476.1"
                     /translation="MEQATFGAGCFWGVESFFREVPGVTDAVSGYAGGHTANPSYRDV
                     CSDRTGHAEVVQVTFDPARVSYDKLLDVFFMNHDPTQLNRQGPDVGTQYRSVIFVHSP
                     EQEKAAQAKKQALGGRFRKPIVTTIESAPAFWRAEDYHQRYFEKNGLPSCHVRMAEES
                     "
     gene            10948..>12112
                     /locus_tag="VG274_07200"
     CDS             10948..>12112
                     /locus_tag="VG274_07200"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWE06477.1"
                     /translation="MSLVARYAGVALAMATILTATTAFGELGRGDRYSGAPWATRSPV
                     LAQHGMVATEQPLASELAIGILKKGGSAVDAAIAANAAIGLMQPVLNGIGGDLFAIVW
                     DPKTKTLYGYNGSGRSPMGRDLAKLTGEVTAAWAKAGEPIKPHIPPVGSLPVTVPGTV
                     DAWFALHDRFGKLPIADDLAPAISYANNGFPVTQLIAHYWKGNVAAFEKSKALIEDLD
                     NARATYLVNGHTPAEGEIFRNPDLAHTLAQIAKGGRDVFYKGAIAHTIDAYFKRIGGD
                     LRYADFAAHHGEWVQPLSVNYHGYDVYELPPNGQGAAVLQMLQILKGFDLKKMGAGSA
                     DTLTAMLEAKRLVYEDLAKWYADPAFVKLPMKQLLSDSYATERRKLIDLRHANR"
BASE COUNT         2287 a         3921 c         3668 g         2236 t
ORIGIN      
        1 ggcgtcgaag ccgccatgca aacggtagcc ggcctcgagt tggggccgcg cataggcgtg
       61 ctcggcgaga atttcgtcgg tgctgtagcg cagttttgcg gtcgccattc cccttgctcc
      121 cgttccgcaa tccggaatga taatttgtca gtcgcgcctg cgagtcaatc tcgcgggctc
      181 ggtcaggcgg aaagtgcccg cacgaggaag cgggtgaggt tgcgcacgac ggcctggcgg
      241 ctgcgcccgg ctcgggcgcc ttcgacggca gtctggtgaa tggcgcccag tatcgcctgt
      301 ccgacgatat ccgcatcgta aatgtcggcg atccggttct gcttgacggc ggcgcggatc
      361 gtttccgccc aatccgctcc ccagcgctgc agcagcggca ctgcctgcgc gccctccgcg
      421 tcgatgacaa gctcatctgt catcgcagca ttgaggacgc cgggatgggt ttccgaatat
      481 tcgaaaatcg cgtgcaacgt gcccgcgatc agttcttcga gcgtctcatg cgcggcgacg
      541 cgctggcgca ggaaggaatc gagttcctgc cttgcgtctt cgacaaaatc gaggaagcag
      601 gcgcgcttgt cggcaaagtg aagatagaaa gtgccgtggc cgagccccgc ctcgcgcgcg
      661 atgtcctgcg gccgcgtcgc gtggtagccc cgttcaacaa acagccgacg ccctgcccgt
      721 ttaagctttg cgagactgtc gggcttgcgt cgcgaggcaa ggggttcaac gggagcattc
      781 agggttgtcg caggttccat gccgaaatct cgtcaccctg ctatacgaat gtcaaatcag
      841 cggggcctgg gtgccgcgtc gaactcactc gtacccaaaa aggttgtcat ggcccgccac
      901 cccgatccgc gcgagagctc ggtcggcgtg taaactccga gcgggccacc caggcgaagc
      961 cgctcggagc gtaaattcag cgcgcggacg cgcgctggct gggtggccac gtcaagcggg
     1021 gccatgacaa ttcgtttgtg attcaattgg cagaactgaa cacccggaac gtggtgcctt
     1081 cctcagtgcg cgccagacgc gctccggcgt cgccggcatg tcgaggtgat cgatgccgat
     1141 cgcgtcagcg actgcgctca ttacggctgg taaagctcca actgttcccg cttcgccgca
     1201 acctttcgcg cctattgcgt gcgtcttgca ggggaattcc cggtacgcga accggatgtc
     1261 cggaatatcc gttgcgcgcg gcagggcgta atcggtgaaa ctcgccgtga gaatttggcc
     1321 cgtctccgca tcatagacgc agttctcgat cagcgcctgg ccgagtccct gcgcgacacc
     1381 gccatgcacc tgtcccgcaa cgagcatggg gttgataacg cggccaaaat cgtcgagcac
     1441 gtgataggag agaacttcga ccacgccggt ctccggatcg atttcgactt cgcagatgtg
     1501 gcagccgttc ggaaatgtcg gcgctgtgcc gcgatattga ccgttactgt cgaggccctc
     1561 ttcgaaaccc acgatagtct cccgcttcag cgtgagcgcg agttcgggca tggtgatcgc
     1621 gcgcaatgtt ccctcgacgc gaaacgtgcc gacgccttcc gagattttga atccgatcga
     1681 tgcgccgccg gcctgcagca cgtgggcggc cgcggccttg cccttgcgga tcgcttcgtc
     1741 ggacgcaatt tcgagcgctc cgccagccat gttgagagaa cgcgatccgc ccgtgccgaa
     1801 tccgctcgcc cagtcggagt caccctgttt gacgatcacc gattcgaacg gcacgccgag
     1861 gcgctctgcc acgatctgcg cgaacgccgt ctcatgcccc tgcccgctcg attgcgtggc
     1921 cacataggct tccaccacgc cattgtcggt gaaatttacc gaagcgggtt cgccgcctgt
     1981 cgccgcggta atctccacat aataggcgat acccagcccg cgccgccggt cgcgctcctc
     2041 cgattttcgc cgccgctgtt cgaaacctgg cacgtccgcg acgtccgccg cctcgtcgag
     2101 catgagggga aaattgccgg aatcgaattc gagtccgaac cagttccgaa acggcaattc
     2161 ttccggcgca ggcagattgc gccggcgtat ctcgatgcga tccaggcccg tcaccgaagc
     2221 cgccttgtcc atcagccgct ccatcagata ggcggcttcc gggcggccgg cgccgcgata
     2281 cgcatcgacg ggcgcagcat tcgagaaata gcctttgatc tccgcgtgga gatgcggcac
     2341 gcgatagacg ccgccgaaga tgcgccctcc cgcgagcgtc ggaatgaatg gcgcaaattg
     2401 cgggagatag cctcccatgt tcgcctcacc accgatgcgc attgcgagga tcccgccatc
     2461 ggcgtcgaga gcgagttcgg cgcgcatgcg catatcgcgg ccgtgagcgt cggcgaggaa
     2521 cgattccacg cgctcagccg accagcgcac cggacgtccc aaaacctttg ccgcaatcaa
     2581 caccagcggc tgctcaggat agatgaagcc cttcatgccg aaaccgccac ccacatcggg
     2641 cgtgagagcg cgcagcttct gggcttgaat gcccagaacg cccgcaacac ggtcacgcac
     2701 gccacccgcg ccctgggagc cggtgtacag cgtgtagcgg tcgcgctcga tatcgtagag
     2761 gccgatcgcg ttgcgtgttt ccatcggatt ggcgcagacg cgattttgca cgatttcgat
     2821 cgcagcaacg tggacggctc tcgcaaatgc ctctccacag gccttttcat tgcccgccgc
     2881 ccagtcgaag acgaggttgc ccggcgcatc atcccaaatc tgcacgcttg ccggcgccgc
     2941 atcgagcgtg cctcccgcat ccagcggctc gtattcgacc atcaccaatt cggccgcgtc
     3001 tttcgcctgc gcataggtct cggcgacgac catcgcgaca gcttcgcccg caaaagtaac
     3061 ccgatccttc gcgagcaagg gcttgggcga gtttttcgtg gccgtaccgt cgcggctgac
     3121 gacgggcgcc atgcacggca tggggcccgc accgaacgtc tcgacatcct cttgcgtaag
     3181 cacaccgaca acgcctgcag cgacccgcgc tgcctcgaca tcaagggcgc caagacgcgc
     3241 atgggcatgc ggacttctga caaaacaggc gaaagcctcg ccaggaaatt gcagatcgtc
     3301 cgtatactga ccgtacccgg tcacgaagcg catgtcttcc gtgcggcgca cggactgccc
     3361 tatgccgaaa cgcatctcgt tttccgtctt tttatgaagc gcgaaagtga tctgtcgcga
     3421 tgggtgctgt caattctcgc gggaccgcac gcgttttacg gcgtcacacc agccttcgta
     3481 gagcgcgtcg cgggtttgac ctgccagttt gggttcgaag cggcgatcca gcgaccacgc
     3541 ggaggcgaca tcggcaaaat cccgaaacag ccccgcgcca agacccgcaa gagaagcggc
     3601 acccagagcc gtcgtttccg tcagggccgg ccgcgccacg ggcagacccg tcaagtcggc
     3661 aagccgctgg cagaacgcat cattgcgcac catcccgcca tccaccttca gaatttccgg
     3721 ccgtccaaag cctgccagtt ccatgtcggc gttcatcgct gcgagaagat cgcgtgtctg
     3781 gaagcagacc gcgtcgagcg cggcgcgcgc gatctccggc gcgccggcgt cacgcgtcag
     3841 accaacgatt gcgccgcgtg cttgcggatc ccaatagggg gcgccgagtc ccgtgaaggc
     3901 tggaacaaag tataacccct cacatccccg ggcgcggcga atgagcgctt cgatctccga
     3961 agcactctcg atcacaccca atccgtcgcg cagccactgc acgacgctcc ccgccacaaa
     4021 gatactgccc tcgatggcat aagctgttgt tccccacgca cggtacgctg ctgttgcaag
     4081 caggcgattt cgcgaggccg gcacggcgat accagtattc accagcgcga aacagccggt
     4141 cccgtaagta gccttgacgt cgcccggcgc aaaacaggcc tgtccgaaca tcgccgcctg
     4201 ttgatccccc gccacaccgc aaatggggat gcgcgccccg agcaggtctt ccgtcgcatt
     4261 gccgaaatcg cccgcgctgt cgcggacttc cggcagaacg acgcgcggca cgtcgaatag
     4321 tgcgagcaac tcgtcgcacc agtcgagcgt gcgcaagtca agcagcattg ttcgcgacgc
     4381 attggttacg tcggtcgcgt gaacgcggcc accggtgagg cgaaagatga gccaactgtc
     4441 gattgtgccg aaggcaagct cgcccgcccg ccccctggca ttcgggacat gattgagcag
     4501 ccattgcagt tttgtcgccg agaaataggg atcgatcacg aggcccgtgc gcgcggtaac
     4561 caacggctca cgaccatttt ccttgagaat gcggcagaga tccgtagtgc gccgatcctg
     4621 ccagacgatc gcagggtgga gggctttgcc ggtcgatcgc tcccacagca cagtggtttc
     4681 gcgctggttc gtcagcccga tcgcggcgat ctctgcaggc gagactcctt tggccgccac
     4741 gcggcagcag gcgatgaccg cctgccagat ttcttcggga tcctgctcga cccaaccgtc
     4801 ggcggggtag atttggcgaa cctctatggc gtgagacgcc aagggccgca gcgaagcatc
     4861 gaaaagaatg gcacgggtgg acgtggtgcc ctgatcgatg gcgagaagaa agggcggttc
     4921 cgcgtccatg cgattcgcga tgcttagcaa atgttcatat taacatctta ttaacgtgct
     4981 taacatagtt tcgccatggg gcgcggcaga aggctgccgg gcagcggggg cgtatgttgg
     5041 gtattcttgc gagaagtctc ggtgaggcga gctgtgtgtc ggcgctcgcc gcaaattcac
     5101 gcaaacaaag cgcgatctgg ctctcggtcg cgacgctttt ctccaatcag gcgccctgtc
     5161 tgcatacgcg cgaacgctcg ctgatgcgcg atattcttcg gcgtctggcg ggtaccgccg
     5221 atgctgcgca gcgaattgtg cttgcggagc gcatcgccga cgaccccgca gttccactcg
     5281 aacttgttct gcacctcgtc gatgatagca tagagattgc agtaccgctc attttgcgca
     5341 gtgcggcgct cgacgaagaa aatctcctgg cactgatcgc tcgcgccgat gtcacccgca
     5401 agattctatg cgcgcaacgc gcgaggattg gcgagcgggt cgcggcagcg ctcgcaaaaa
     5461 gcgacgagga atgcgtgctt ttggccctcg ttcacaattc gaccgcgcaa atctccacca
     5521 cgatcttcga aatcctggca gaaaaatcgc gtcgcttcga gagcctgcgg gagccgcttg
     5581 ccaggcgctc cgaccttccc gcgatcctgt ggacaaaaat acgcgaaggg ctgccggagg
     5641 cgacaaagag cctgtccgaa aagggcccgg ttgtgccgcg accgaatgac tcgccggaag
     5701 catcggacaa cgcccgcaag cttatcgaca aattggcggt cgccggacag ctgggggcgg
     5761 gatttctcat gcgcgtgctc ctgcaagggc agaccgagct gttcgatatc gctttcgcca
     5821 gattgctcga ccttggactt gaggaattcc gcgctgcgtt ttacgcgagc gacccgcaag
     5881 gcatggctct ggcgtgccgt gccgtcggca tcgatcgtag cgtttttcca accgtctggg
     5941 ggttgtccac gaaacgccgc gccggtcccc gactgtttgg ttccggccga ccggcagccc
     6001 tcgacagcct gtttgcgctg cccaagccgg aagcccgtgc ccgtcttcac gcctgttcct
     6061 gatatttgcc aattcaagac tctgacgaaa gcctgtacac tgcccggatg atcgatccct
     6121 tcgggcggca catcacctat ctgcgggtct ccgttaccga ccgttgcgat ttccgctgcg
     6181 tctactgcat gccggagcac atggacttcc tgccgagggc cgaactcctg acgctcgaag
     6241 agctggaacg cctgtgccga gccttcatgc gcaggggcgt ggagcgaatc cgcctcaccg
     6301 gcggtgagcc tctggtgcgc cgcgacatca tgcggctcat cggccgcctc ggggcggcag
     6361 tgggccgaga cctgcgcgaa ctcacgatga cgaccaatgg cagccagctt gcccgcttcg
     6421 ccaaggatct tcgcgccgcg ggcatgcgcc gcgtcaacgt ttcgctcgac acgcgcgacg
     6481 cggacaaatt ccgcaccatc acccgccggg gcgatctctc gaaagtgctc gccggtatcg
     6541 acgcggcgca gagcgccgga ctcgaaatca agatcaacat ggtggcgctc aaggatgtga
     6601 acgaggatga aatcgagtcc atgctcgagt gggcgcatgg gcggggaatg ggcctgacgc
     6661 tgatcgagac catgcccctc ggagagatcg acggcgaccg cgtcggacaa tatctgccgc
     6721 tatcgatcgt gcgcgcccgg ctagcgcaac gttacacgct ggaggaaagc gaattccgca
     6781 ccggcggccc ggcgcgttat gtgcgggtgg gcgaaaccgg cggcaggctc ggcttcatca
     6841 cgcctctcac ccataatttc tgcgaaagct gcaaccgcgt tcgtctgacc tgcacgggga
     6901 cgctttacat gtgtctcggc caggccgaca gcgcggattt gcgctctccg ctgcgctctt
     6961 cagaggacga cgcactgctc gatcgcgcca tcgacgcggc gatcgcacgc aagccgaagg
     7021 gtcatgattt catcatcgac cgtcgccacc agactcccgc tcttgccaga cccatgtcgg
     7081 tgaccggagg ctgatcccgt gccgcggtcc ctgcacttca cggcctccga cgcggccgaa
     7141 gcgcaggagg cgctccggcg catgcgccag tcctacggcg atgcaggcgc cgatcatgcc
     7201 gagattgtca tcgcactggg cggcgacgga ttcatgcttc agaccctgca tgcatttctc
     7261 gatcgcaacc ggcccatcta tgggatgaat ttcggctcga tcggatttct gatgaacgaa
     7321 tatggcgagg aacagctgca cgaacggctg gacgcggcgg agtcggcaag aatccatccg
     7381 ctgcgcatgc acgccgcgac gcgcgcaggc aaaaaagtcg aggcgctggc tttcaacgag
     7441 gtttcccttt tgcgcgagac gcggcaggcc gcaaaaatcc gcattcttgt cgatgacaag
     7501 cccaggatcg ccgaactgat ctgcgatggg gtgctgatat cgacgcccgc cggcagcact
     7561 gcctacaatc tgtccgcaca cggaccgatc ctgcccatcg atgccgatct gctggcgctg
     7621 acgccgatca gcgccttccg gccgcgccgc tggcacggcg cgcttctctc gcatcgtgcc
     7681 cgcacccggt tcgaaatgct cgaagctgac aagcggcccg tcagcgcggt cgcggacgat
     7741 ctcgaggtcc gcgacgtgcg ttcggtcgac gtggccgagg atcgctcgat caccatgacg
     7801 atgctcttcg atcaggggca taacctcgac gagaggattc tggccgaaca gttcgttgac
     7861 tgatccgcct gtaccagggc agtcagactc cactcacctt tcgtcgccca cggcgattcg
     7921 cgtcgtttcg tagccggcgt cgtcgcggat gcggaaatac ttgccgtcgg gtccgtcttc
     7981 gagccagggc tcgacggtct gcggcggcgt ggcacgcgcg gcggcgagcg cttcggccac
     8041 cgttcccgtt ttcgccagct tcatgagatt gagtttcgtc ggaaacatga tgttccattg
     8101 tctccgcatc gcgatcgcct gctccggccg cgcccacagg gaatccaccg actcgcggcc
     8161 gtcgtgccgg cccgtatgat ccgtaggcgc acgggcgaga aagaaatggg tgtcgaaacg
     8221 tttcggcatg gatttcggcg ttatccagtg cgcgaagtga acgagctcat cgcaggccag
     8281 tgtcagccgc tcaattcgaa gcatctcgcc aagactggtt tgcccaatct cgattttctc
     8341 acgatacgga tcgagagcgg ccagccgttc gccggccacg atcttccccg tgcggcagtc
     8401 gcgtgcgagc agaacgccgg cttcctcgaa ggcctcgcgg atagccgcaa cggcaaccgc
     8461 cctcatgggt ggcgaccagt cggatgcgcc gcccatgcga ccggcgagac tcgcatcgga
     8521 atcctcgcga cacgcctttc cgcccgggaa caccagcgcg ccggccgcaa accccatttc
     8581 atggtggcgt ttcaccatga aaacttcgat gccgtcgccg ccgtcacgcg caagcagcat
     8641 cgttgcggcg ggaactggaa caacctcctt ccgcgatttt tgatcggaca tgattcctcg
     8701 caattctgcc aaacctagcg cggcaatcga cgaaatttaa atctcttcgg catgaagcct
     8761 cttactccac ggcttctgcc gggactggcc acgcgccgtc aaattttgcg gcgcagattt
     8821 tcgatgagat cgtcgcggac agatgattcg tagcgcaacg gcgcgccgtg tccccagatg
     8881 ggacccggcc acgcggcatc tcctggatgc ctggcgacga tgtggacgtg caattgcggg
     8941 acaacattgc cgagcgctcc gatgttgatc cttgtggcct ttgtcatttt tcgaagccca
     9001 atgctggcac gggcgatttc ctcgatcagg atggaccgat ccgatgaatc gagttcgtga
     9061 atctccgaaa tccccgtgcg gcgcggaacg aggatcagcc agggaaatcg cgcatcgccg
     9121 gcgagcagca gccgcgacag cgtccaatcg ccgacaagac agctttctgc ggcaaggcat
     9181 tcgtgaagct gaaacaccgg aacaccggct ccatatacct atccaggcgc ctgttccctc
     9241 gcggcacttg ctccgaacct gcgtggatcg aaggcgccga tatcgggcgg aagggtctcg
     9301 cctaacacaa tcgcacgcat tgcgtcggcg accgccggcg caaacagaat gccgttgcgg
     9361 tattgtcccg aggcgatgaa gaggccatcg atcgcgctcc tccccaacag cggcagatcg
     9421 tcgggcgagc cgggccgtaa tcccgcccaa tgttcgacga caggccaatc gcgaagcgat
     9481 ggcatcagcg ccaccgcccg atcgatcagc catcgcgccg ccccttcggt cagacccgtg
     9541 tcgaagcccg cgcgcgaaac cgttgcgcca accagcagac gatcgcgctg cgcgacgagg
     9601 tacgcgtcct ggcccccgat tgaaatccgc gggagagttt cgccagccgg cggttgcagc
     9661 gccagcatct ctcccttgac cggaaacacg ggcggcaccg cttcgcgtgg cagaccctcg
     9721 atgcctgcac tccacgcgcc ggccgcgacg acataggcgt cggcctgatg cagcgcgaac
     9781 ggagtccgga tcccaatgat tcgctctccc tcgatttcga agcacacagc cgtctcgttg
     9841 agaagcagcg agccaccgga tttcacgaag catgccgcaa gcgcagggcc gagcgcacga
     9901 ttgtccacgc gggcatcgtg cctgtcccac aatgcgcccg ccaaatcggc cgcgagtctc
     9961 ggttcgatct cgcgcgcggc ctcggcctca agcatctggc cggctccgag ctcagcctgt
    10021 gcagccagcg ccgcatacat ttccgcggtt tgcgcaacga caagtctgcc atcgacccgg
    10081 taggcgatgg tgcgtccgct ttcccgctcg atttcggtgg caaagtcctg ccacagtcgt
    10141 gccgaacgtt gcgcaaagcc ggcttcccgg ccagcgcctt cggctttgtc ttctttcgcc
    10201 gttatcaggc cggccgatgc ccatgtcgcg ccgcgaccgg gctgcgcgcg ttccagaagg
    10261 accacctcga cgccacgctg ggcgaggcgc catccgattc cgagccccgc gactccgccg
    10321 ccgatcacaa caactttcat gccaggcaat tctcgcaagc cgtgaaccga agtctttgcc
    10381 gggcttaggc tcgcgggtag cgaaggtcaa atcgctctcg tctgcccgca cattggtact
    10441 atattggttt gccgtcaggg agatcgatat ggaacaggcc acgtttggag cgggttgctt
    10501 ctggggggtg gagtcctttt tccgcgaggt gcccggcgtg acggatgccg tatccggcta
    10561 tgcgggtgga catacggcga acccttccta tcgggacgta tgcagcgacc gtacgggtca
    10621 tgcggaggtg gtgcaggtca ccttcgatcc ggcgagggtt tcctatgaca aactcctcga
    10681 cgtgttcttc atgaatcacg atcccacgca gctcaaccgc caggggccgg atgtgggaac
    10741 gcaataccgt tccgtcatct ttgttcactc gcccgaacag gaaaaggcgg cgcaggcgaa
    10801 aaaacaagcg ctgggcggcc gattcagaaa accgatcgtc accacgatcg aatccgcgcc
    10861 cgcgttctgg cgggccgagg actatcacca gcgctatttc gagaagaatg gccttccatc
    10921 gtgccacgtc cgcatggcgg aagaatcatg agtctcgttg cgcggtatgc cggagtagcg
    10981 ctcgcgatgg caacgatcct caccgcgacg acggctttcg gcgaactcgg ccgcggcgac
    11041 cgttattcag gcgcgccgtg ggccacccgt tcaccggtgc tcgcgcagca cggaatggtg
    11101 gcaactgaac agccgctcgc ttccgagctc gcgatcggca ttctcaagaa ggggggcagc
    11161 gcggtggatg ccgctattgc ggcgaatgcg gccatcgggc tgatgcagcc cgtgctgaac
    11221 ggaatcggcg gcgatctctt cgcaatcgtc tgggatccga agacgaaaac cctgtacggc
    11281 tacaacggct cgggccgctc gccgatgggc cgcgaccttg cgaagctgac cggcgaagtg
    11341 acggcggcct gggcaaaggc aggcgaaccg atcaagcctc acattccgcc ggtcggttcg
    11401 ctgccagtta cggtgcccgg tactgtcgat gcatggttcg cgctgcacga ccgattcggc
    11461 aagttgccga ttgcggacga tctcgcacca gccatctcgt atgcgaataa tggttttccg
    11521 gtaacgcagc tgattgctca ctactggaag ggcaatgtgg cagcgttcga gaagagcaaa
    11581 gccctgatag aagacctcga caatgcgcgg gcgacctatc tcgtcaacgg tcacacgccc
    11641 gccgaaggcg aaattttccg taatcccgat ctcgcgcaca cgcttgcgca aatcgccaag
    11701 ggcggtcgcg acgtatttta caagggcgcg atagcgcaca cgattgacgc ctatttcaaa
    11761 cgcatcgggg gggacctgcg ttatgccgac tttgccgcgc atcacggcga atgggtgcag
    11821 ccgctctcgg tcaactatca cggctacgac gtctacgagc tgccaccgaa tggccagggc
    11881 gcggccgttc tgcaaatgct gcaaatcctc aaggggttcg acctcaagaa gatgggtgca
    11941 ggatcggcgg acacgctgac ggccatgctc gaggccaagc ggctcgtcta tgaggatttg
    12001 gcgaaatggt acgcggaccc cgcgttcgtg aagttgccca tgaagcagtt gctctcggat
    12061 tcttacgcga ccgagaggcg caagctgatc gatctgcgcc atgcgaaccg gg
//
LOCUS       DASZMC010000353         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4285014,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000353 DASZMC010000000
VERSION     DASZMC010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4285014"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..434)
                     /gene="yacG"
                     /locus_tag="VG274_07205"
     CDS             complement(231..434)
                     /gene="yacG"
                     /locus_tag="VG274_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HWE06478.1"
                     /translation="MSPRKPEALARRCPICGRPRTEKFKPFCSKKCSDIDLARWLSGS
                     YAIPAQENDDADSEPSVDTEDKT"
     gene            complement(415..2043)
                     /locus_tag="VG274_07210"
     CDS             complement(415..2043)
                     /locus_tag="VG274_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWE06479.1"
                     /translation="MKKEILINAGAGEVRVAIVEDGRLQEVFLERTIGLGDGTQRRRL
                     ADCGGHSLIGNIILGRVQRVLPGMQAAFVDIGVDRAGFLGAREARCLADVGMVESSGF
                     DSAAPVASARENGASPCPWRHARPGDAATEERAPRIGDCVREGEEILVQVVKDPIGDK
                     GARLSANVTMPGRLLVLVPNQPGIALSRRIDDEAERVRLASLCEQMVAESQATLAAGA
                     GYIVRSAAIGAEFAELREDALRLAEAWLPVAATRKLARAPTTLHHDLDPIERMMRDEV
                     DAATSRVLIDDQEALAAARAYCRRAMPEAEGRLELFRNAGMLFDLYDLEDEIERLVSA
                     RVALPSGGWITIESTEALTAIDVNSGSFTSAAGLEETSLRVNLEAASEIGRQLRLRGI
                     GGLIVIDFIHVGGATGVDKVLAALNESLSRDRTPTQILPMSEFGLVEITRKRIRDPLS
                     RMVTESCRACRGRGRTRSCETVALEILRRVEREALHAPGRPIVVRAAPEVVYWIETHG
                     AELRAGLARRNVFRVIFEPRSEFAREGYDVSAQA"
     gene            complement(2087..2662)
                     /locus_tag="VG274_07215"
     CDS             complement(2087..2662)
                     /locus_tag="VG274_07215"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWE06480.1"
                     /translation="MLASASPRRQTLLAQAGVVPDAICPADVDETPVKGELPRRHALR
                     LALLKAESVAHEWRGEPALVLAADTVVACGRRILPKAQTEGDVRACLELISGRRHLVF
                     TAVAVQRAHAPVPVRSKIVATRVAFKRLSRREMDAYAASGEGQGKAGGYAIQGRAEAF
                     VRQLNGSWSNVVGLPLLETLGLLESFGYAAG"
     gene            complement(2676..2894)
                     /gene="infA"
                     /locus_tag="VG274_07220"
     CDS             complement(2676..2894)
                     /gene="infA"
                     /locus_tag="VG274_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWE06481.1"
                     /translation="MAKEALVEFPGTVSELLPNATFRVTLENGHEIIAHTAGKMRKNR
                     IRVLTGDKVLVEMTPYDLSKGRITYRFK"
BASE COUNT          534 a         1004 c          943 g          536 t
ORIGIN      
        1 tcagtccgct gctctaccga ctgagctacc tgggcaggct tccggcgggc cggaagggac
       61 gggcttttta gaagaggggt gcgacggtgt ccagcgggcg cgaggcacgg cgaatgaggt
      121 cgctgatcac gccagcgtcc aaagctcgtc atggccacgg cccggccatg acggaactac
      181 atggaatcgc gaatccgctc tgctccgcgc gaagtgttgg aatcgaaccg ttacgtcttg
      241 tcctccgtgt cgactgatgg ctcggaatcc gcatcgtcgt tttcttgtgc ggggatcgcg
      301 tagctgccgg agagccagcg cgcgagatcg atgtcggagc atttcttcga gcagaatggc
      361 ttgaactttt ccgtccgcgg acggccgcat atggggcagc ggcgcgccag tgcttcaggc
      421 ttgcgcggag acatcgtagc cctcgcgcgc gaattcgctg cgtggttcga aaatgacgcg
      481 gaaaacattg cggcgcgcga gtccggcccg cagttcggca ccatgcgtct cgatccagta
      541 aaccacttcc ggcgcggcac ggaccacgat cggccggccc ggagcgtgca gcgcttcgcg
      601 ctccacccgg cgcagaattt ccagcgccac ggtttcgcag gagcgcgtgc gtccgcggcc
      661 gcgacaggcg cggcagcttt cggtcaccat ccgcgagagc ggatcgcgaa tgcgtttgcg
      721 cgtaatttcc accaagccga attccgacat cggcaggatc tgcgtgggcg tgcggtcgcg
      781 cgacaggctt tcgttcagcg cggccagaac cttgtcgacg ccggtggcgc cgccgacatg
      841 aatgaaatcg atcacgatga gcccgccaat gccacgcagg cgaagctggc ggccgatttc
      901 gcttgccgct tcgagattga cccgcaggct cgtttcctcg agcccggccg ccgaagtgaa
      961 actgcccgaa ttcacgtcga tcgcggtgag cgcctcagtc gactcgatcg tgatccagcc
     1021 gcccgagggc agcgccacgc gtgcgcttac aagccgctcg atctcatcct cgagatcgta
     1081 aaggtcgaag agcattcccg cgttcctgaa caattcgagc ctgccttcgg cttccggcat
     1141 cgcgcggcgg caataggcgc gcgccgccgc gagcgcttcc tggtcgtcga tgagcacgcg
     1201 gctcgtcgct gcatcgacct cgtcgcgcat catgcgttcg atgggatcga gatcgtggtg
     1261 gagcgtcgtg ggcgcgcgcg ccaatttgcg tgtcgccgcc acgggcagcc aggcttcggc
     1321 gaggcgcagc gcatcctcgc gcaattcggc gaattcggcg ccgatggcgg cgctgcgcac
     1381 gatatagcct gcgcctgccg cgagcgtcgc ctggctctcc gcgaccatct gttcgcacag
     1441 cgaagcgagc cgcacgcgct ccgcttcgtc gtcgatgcgg cgggagagcg cgatgcccgg
     1501 ctggttggga accagcacga gcagacggcc cggcatcgtg acattggcgg aaagccgggc
     1561 gcccttgtcg ccgatcggat ccttcaccac ctggacgagg atttcttcgc cttcacgcac
     1621 gcaatcgccg atccgcggag cgcgttcctc ggtcgcagcg tctcccgggc gggcgtgacg
     1681 ccacgggcag ggcgaggctc cattttcgcg agcactggcg accggtgcgg cagaatcgaa
     1741 tcccgaactt tcaaccatgc ctacatcggc aaggcagcgc gcttccctgg cgccgagaaa
     1801 tcccgcacgg tcgacaccga tatccacaaa cgccgcctgc atgccgggca gcacccgctg
     1861 cacccggccc agaataatgt tgccaatcag gctatggccg ccacaatccg ccagccggcg
     1921 ccgctgcgtc ccgtctccaa gcccgatcgt ccgttcaagg aacacttcct gcagacgacc
     1981 gtcttcgacg atcgcaacgc gaacctctcc cgctcccgcg ttgatcagga tttctttctt
     2041 cattccggga cgcgtcgaat gtctgcatta tgacactagc atgaactcac ccggccgcgt
     2101 agccgaagct ttcaagcaga ccgagcgttt cgaggagcgg caggcccacc acattcgacc
     2161 aggaaccgtt caactgccgc acgaaagcct cggcacggcc ctggatcgca tacccgccgg
     2221 ccttgccctg cccttcccca cttgccgcgt aagcatccat ttcccggcgc gaaaggcgct
     2281 tgaacgcaac ccgcgtggcg acgattttcg atcgcacggg aaccggagcg tgcgcgcgct
     2341 gcaccgcgac cgccgtgaag accagatgac ggcggccgga aatcagctcg aggcaggcgc
     2401 gaacgtcacc ttcggtttgc gccttgggca ggatgcgcct gccgcaggcg acgaccgtat
     2461 ccgcggctaa cacgagagcg ggttctccac gccattcatg tgcgacgctc tccgccttga
     2521 gcagcgcgag ccgaagcgcg tgcctgcgcg gcaattcgcc tttgacaggc gtttcgtcga
     2581 catcggcggg acagattgcg tcggggacca ctcctgcctg cgcgaggagc gtctggcggc
     2641 gcgggctcgc gctcgccagc acgaggcgcg gcggattatt tgaaacgata ggtaatgcgc
     2701 cccttggaaa gatcatatgg ggtcatttca accagcacct tgtcgccggt cagcacccgg
     2761 atgcggttct tgcgcatctt gcccgcagta tgggcgatga tctcatgtcc gttctcgagc
     2821 gttacgcgaa aggtcgcgtt gggcagaagc tcgctgacag tccccggaaa ttcgacaagc
     2881 gcttccttgg ccattcatcc tctggcttgt ttctcggcgg ccgggagaat gcaccgaaac
     2941 cgcggaaaag caaggcaatg ggctcaggca tcctctcccc gggagaccgc aaaacggcgc
     3001 tcgatatgct ccatcag
//
LOCUS       DASZMC010000354         7603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2897601,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000354 DASZMC010000000
VERSION     DASZMC010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2897601"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1226
                     /locus_tag="VG274_07225"
     CDS             30..1226
                     /locus_tag="VG274_07225"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWE06482.1"
                     /translation="MRGPLDGIRVVDFGRYIAGPFCAALLGDLGADVVRVEKREGRED
                     RTMIPLGETPEGAPREGALFLQMNRNKRGLTLDPATPGGREIVRRLLAASDVVVANLP
                     RATLAALGLDYQSVCAINPRAILAVVSAFGLDGPYADRVGFDGVAQAMCGSAWFSGTE
                     DQPMRAAAPWVDFGTASLLALGVVAALRARDRSGRGQLVEGALLRTAISFFSPMLIEE
                     SLLKLARQPTLNRSQTAAPSDIYRTRDGWVTIAVNGDPLFRRVAKLIGAPEWLEDARF
                     ASDKTRGDHGAIVSARMSQWCAQRSASDVISACEAARVPAGPVYRMRETLDDPHVRAS
                     DILTEIDFPGIGTAAIAATPVKLHATPGDVRRRPPLLGEHNTEILGELGFTSAEIASF
                     RKDGTV"
     gene            1513..1800
                     /locus_tag="VG274_07230"
     CDS             1513..1800
                     /locus_tag="VG274_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWE06483.1"
                     /translation="MEIVGFRHKGLERFWRTNDSKGISSRWTEKLRAMLTAIEEANDV
                     TEVGLLPGWKLHPLKGSKRGAWSMWITGNQRLTFLVDGDTVSELNIEDYHG"
     gene            1806..2108
                     /locus_tag="VG274_07235"
     CDS             1806..2108
                     /locus_tag="VG274_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWE06484.1"
                     /translation="MTKMKNPSHPGDLVYWGILEPLDIAIGRAAELLGVRRATLSDLV
                     NGKSALSAEMAYRIHKAFGPDPDMLMRVQAAYDMAQFRNTKQARNIKVKRYHRKAA"
     gene            complement(2312..3646)
                     /locus_tag="VG274_07240"
     CDS             complement(2312..3646)
                     /locus_tag="VG274_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWE06485.1"
                     /translation="MWTLLGLLVGGTALSGVFRSSVEGNFDARLKSDLDEMIAAAEPN
                     AQGEVSLQGRFADPRFERVYSGWYWEITPEGPKGPQTNDAQISRSLWDRTIKVADFRR
                     SRTLVWGHGEGPDNQKLRFVAQRIEFPVTPGEPKAGMRSFSLLVAGDEAELEKEVSDF
                     NGTLFWSFAVLGFGLMGAIFVQVRIGLQPLNRVSEALSRIRDGKARRLDGKFPAEIAP
                     LVGELNSLIAHSAEVVGRARTHVSNLAHFLKTPLTVLASEAGAHPGALADAVLRQVAT
                     MRRQVDHYLARARAAGALDVLGNRTPVRPAIEDLARVLSRIHAARGLAIDVDCPPDLA
                     FRGERQDLEEMAGNLIDNACKWAHARVAVAARRGEGTRFELRIGDDGDGIDPAERIKV
                     GERGERLDETVPGSGLGLAIVRDIARLYGGTLTLADSPLGGLEARLTLPAIA"
     gene            complement(3714..4403)
                     /locus_tag="VG274_07245"
     CDS             complement(3714..4403)
                     /locus_tag="VG274_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWE06486.1"
                     /translation="MRILLVEDDQDLQRLLKRALQDAGYVVDSASDGEEGHFLGDTEP
                     YDAVILDLGLPKMDGVRVLQQWRKAGRSMPVLILTARDRWSDKVAGFDAGADDYLAKP
                     FYTEELLARVRALLRRAAGFATSEIEIGALRIDTRASRVTLDGNPVKLTSLEYRLLAY
                     LAHHRGRVVSRTELVEHLYDQDFDRDSNTIEVFVGRLRKKLDANLIQTVRGLGYCLDT
                     PDAAQQAHSPR"
     gene            complement(4624..5061)
                     /locus_tag="VG274_07250"
     CDS             complement(4624..5061)
                     /locus_tag="VG274_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06487.1"
                     /translation="MFRRAIPAVFLALAALASPASAQPRHVVPYGTNVQPLDRILPGI
                     RNGRPGRFYDAEGPYADPTGGWHYRLKWLTPDGRVQWLDTDARTGRVLGPYRGYGGMA
                     MPPSGLRYYPYPGPPRGGFPGGFRGGFRGRFGGWRPGGHGRGR"
     gene            5636..5953
                     /locus_tag="VG274_07255"
     CDS             5636..5953
                     /locus_tag="VG274_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06488.1"
                     /translation="MQFIDSASQYLYRDFSVVASKATATNSGCGTAISSTFATGLSPA
                     TQHGGVVENLYVGTDGVTTGDPDSAVAWSHFNIGMDLTGTHRPFVFNTLFSGCTGKGV
                     KGN"
     gene            6035..6892
                     /locus_tag="VG274_07260"
     CDS             6035..6892
                     /locus_tag="VG274_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06489.1"
                     /translation="MWHASTAITRSSMGGNVQGFSVIDTHIEGVKTGIFHQHGTTTAG
                     EDPDGKLIGCSVQYRDVGFDIANVSKWRVALNGFRNEWDGSPSETPVYDMRFRAANVV
                     SLVQNTFAVGGNPARVNMLIDADSTMNHVAHAMRFLIDAHQFGDNTSGEGASFFGISA
                     IQITASATDIQIGASNQYLGPFSGAIVDDASGAAVTLAGFPGVPGDPTQPGVVAPSSA
                     DPAAGITTLGSGSGPWPASFSLGHYLPTNSSGITPVPPTTGWVFYTDSTGKLYAQYRD
                     NGPILLLSP"
BASE COUNT         1390 a         2447 c         2409 g         1357 t
ORIGIN      
        1 ggacgtctag catcggcaaa gagggcacaa tgcgcggtcc attggacgga atcagagtgg
       61 tcgatttcgg ccgctacatc gcaggaccgt tctgcgcagc gctgctcggc gatctcggcg
      121 ccgatgtcgt ccgggtcgag aagcgcgagg gacgcgagga ccgcacgatg atcccgctgg
      181 gcgagacgcc cgagggcgcg ccgcgcgaag gcgcgctgtt tctgcagatg aaccgcaaca
      241 agcgcggtct gacgctcgat cccgcaacac cgggcggacg tgaaattgtg cgccggctgc
      301 tggccgcaag cgatgtcgtg gtcgccaatc tgccgcgcgc aacgcttgcc gcgctcggac
      361 tcgattacca gtcggtctgc gcaatcaatc cgcgcgccat cctcgcggtc gtatccgcct
      421 tcggcctcga cggtccctac gcagatcgcg tgggttttga cggtgtcgcc caggccatgt
      481 gcggctcggc gtggttttcg ggaacggaag atcagcccat gcgcgcggcc gcgccgtggg
      541 tcgatttcgg tacggcgtcg cttctcgcgc tgggcgtcgt ggccgcgttg cgcgcgcgcg
      601 accgcagcgg gcgcggacag ctcgtcgaag gcgcgctcct gcgcaccgcc ataagtttct
      661 tcagtccgat gctgatcgag gagtcgctgc tcaagctcgc gcgccaaccg acgctgaacc
      721 gcagccagac cgcggcgccg tcggacatct accgaacgcg cgatggatgg gtgacgatcg
      781 ccgtcaacgg cgatccgctg ttccgccgcg tggcgaaact gatcggcgcg cccgaatggc
      841 tcgaggatgc gcgctttgcg agcgacaaga cgcgcggcga tcacggcgcg atcgtcagcg
      901 cacgcatgtc gcaatggtgc gcacaacgca gcgcgtccga cgtgatttcc gcctgcgagg
      961 ccgcgcgcgt gcccgccggt cccgtctatc ggatgcgcga aacgctcgac gatccgcatg
     1021 tgcgggcgag cgatattctg accgagatcg attttcccgg aatcggaacc gcggccattg
     1081 ccgcgacgcc ggtcaaattg cacgccacgc cgggcgacgt ccgcaggcgt ccgccgctgc
     1141 tcggcgagca caatacggag attctcggcg agctcggatt caccagcgcc gagatcgcgt
     1201 cgttccggaa agacggaacg gtctgagcgc gcgctcctgt ttatcaggcg atttacaggc
     1261 gattcgttgt cacaggcggt tcatggcgcg aacggaatcc atattgtcgt tgcgattcaa
     1321 ggccgtcgcg gagtttccat tattccgcgg aacaggcgcg gattcgtctg ttcgccggca
     1381 attctcctgt tattgccttc gcaagcgaaa caaagcgctg acgattgttt cacgggaaac
     1441 attgttgaat gccatcacgc ttacggaggc gatgccattg accttcgtac ggtatagccg
     1501 tacacattgt aaatggagat cgttggcttc cgccacaaag ggctcgagcg cttctggcgc
     1561 accaacgatt ccaaaggaat atcgtcgcga tggaccgaaa agctgcgcgc catgctgacc
     1621 gcgatagaag aagccaatga cgtgaccgag gtcgggctcc ttccgggatg gaagcttcat
     1681 ccgttgaagg gatccaagag gggtgcctgg agcatgtgga taacgggaaa ccagagactc
     1741 accttcctcg tcgatggcga cacggtgagt gaattgaaca tcgaggacta tcacgggtga
     1801 aagtcatgac gaaaatgaag aatccgtcgc atccgggcga tcttgtctat tggggcatat
     1861 tggaaccgct ggatatcgcg attggacggg cggccgaact cctgggagtt cgcagagcaa
     1921 cgctctccga cctggtgaac ggaaaatcgg cgctgtccgc cgaaatggcc taccgcattc
     1981 acaaggcgtt cggccccgat ccggacatgc tgatgcgcgt gcaggcggcc tacgacatgg
     2041 cgcagttccg caacacaaag caggcgagga acatcaaggt gaagcgttat caccgcaagg
     2101 cggcttgagc atggcggcgc tcgaagtgga caggatgggg cgaacgctgc ggtccggtac
     2161 tgaaaagcat gaaattcaat gatgtcgttc ccgcccgatc cccgctttcg tggggatcgg
     2221 gcgggaatga tgtttgtttt gaatcgggcg ccaatgttcg ttggctcccg gactcccgct
     2281 ggagttttac acgccgcgag cgaggccgtc ttcacgcaat cgccggcagc gtcagccttg
     2341 cttcgaggcc gccgagcggg ctgtcggcga gggtgagggt gccgccatag agcctggcga
     2401 tgtcgcgcac gatggcgaga ccgaggcccg agccgggaac cgtctcgtcg agccgctcgc
     2461 cgcgctcgcc gaccttgatg cgctcggcgg gatcgatccc gtcgccatcg tcgccgatgc
     2521 gcagttcgaa acgcgtgccc tcgccgcgcc gtgcggcgac cgcgacgcgc gcatgcgccc
     2581 atttgcacgc attgtcgatc aggttgcccg ccatctcctc cagatcctgc cgttcgccgc
     2641 ggaacgccag atcgggcgga caatccacgt cgatggcgag accgcgcgcc gcatgaatgc
     2701 gcgacagcac gcgcgccaga tcctcgatcg ccggccggac cggcgtgcgg ttgccgagca
     2761 cgtcgagcgc gccggccgcg cgggcgcgcg cgagatagtg atcgacctgg cggcgcatgg
     2821 tcgcgacctg gcggagcacg gcgtcggcga gggcgccggg atgcgcgccc gcttcgctgg
     2881 cgagcacggt gagcggcgtc ttcaggaaat gcgcgaggtt cgacacgtgg gtgcgcgcgc
     2941 ggccgacgac ttcggcgctg tgcgcgatca ggctgttcag ctcgcccacg agcggcgcaa
     3001 tctcggcggg aaactttcca tccagccgcc gcgccttgcc gtcgcggatg cgcgacagcg
     3061 cctcgctcac ccggttcagc ggctgaagcc cgatgcgcac ctgcacgaag atggcgccca
     3121 tcagcccaaa tcccaggacc gcgaacgacc agaacagcgt gccgttgaaa tcggacactt
     3181 ccttctcgag ctcggcttcg tcgccggcga ccagaagcga aaaactgcgc atgcccgctt
     3241 tcggctcgcc gggggtcacg ggaaattcga tgcgttgcgc cacgaagcga agcttctgat
     3301 tgtccggtcc ctcgccgtgc ccccagacaa gcgtgcggct gcggcgaaaa tcggcgacct
     3361 tgatcgtacg gtcccacagc gagcgcgaga tctgcgcgtc gttggtctgc ggccccttcg
     3421 ggccctccgg cgtgatctcc cagtaccagc cggaatagac gcgctcgaaa cgcggatcgg
     3481 cgaaacgccc ctgcagcgag acttcgccct gcgcgttcgg ctcggcggcg gcgatcatct
     3541 cgtcgagatc ggatttcagc cgcgcgtcga aattgccctc gaccgaagag cggaagactc
     3601 ccgaaagcgc ggtgccgccg acgagcaggc ccaagagcgt ccacacggcg gcggcggcga
     3661 tcagccgcgc ggcgagggaa tcgaaccttc gcgcgacccg ctgcgccgtc gcgctagcgc
     3721 ggcgagtggg cttgctgagc cgcgtccgga gtgtcgagac aatagccaag gccgcgcacc
     3781 gtctggatca gattggcgtc gagcttcttc cgcagccgtc ccacgaacac ttcgatcgtg
     3841 ttcgaatcgc ggtcgaagtc ctgatcatag agatgttcga ccagctcggt gcgggaaacc
     3901 acccgcccgc gatgatgcgc gagataggca agcagccggt attcgagcga agtgagcttg
     3961 accggattgc cgtcgagcgt cacccgcgag gcccgcgtat cgatgcgcag cgcaccgatc
     4021 tcgatttcgg acgtggcgaa acccgccgcg cgcctgagca gcgcgcgcac ccgcgccagc
     4081 agctcctcgg tgtagaacgg cttggcgaga tagtcgtcgg cgccggcgtc gaatcccgcg
     4141 accttgtcgc tccagcggtc gcgcgcggtg aggatcagca ccggcatgct gcgcccggcc
     4201 ttgcgccatt gctgcagcac gcgcacgccg tccatcttcg gcaggccgag atcgagaatg
     4261 accgcgtcat agggttcggt gtcgccgagg aaatgacctt cctcgccgtc gctcgcgctg
     4321 tcgaccacat agcccgcgtc ctgcagcgcg cgcttcaaca gccgctgcag atcctgatcg
     4381 tcctcgacaa gaagtatccg catttatcgc ctggaaaaag tttgaacgca gagggcgcag
     4441 aggatgcgca gaggacgcag aggaattttt ggcgcgcttc ctcctacgct gctccgcagc
     4501 ttcggtggac tgccgctctc gcccgccgaa gccttggcgt aggcgggtcg cgcgcaatca
     4561 ttttcctgcg tcccctgcgc atccaccgcg taccctgcgt taaaatcttg ttggtgcatg
     4621 aactcagcgg ccgcgcccgt gtccgcccgg gcgccagccg ccgaagcgtc cgcggaagcc
     4681 gccgcggaag ccgccgggga agccgccgcg cggcggcccg ggatagggat aataccgcaa
     4741 tccgctgggc ggcatcgcca tgcccccata gccccgatag gggccgagaa cgcggcccgt
     4801 gcgtgcgtcc gtgtcgagcc actggacgcg gccatccggc gtcagccatt tgaggcggta
     4861 atgccatcct cccgtcggat cggcgtaggg tccttccgca tcgtagaagc gtccgggcct
     4921 gccattgcgg attccgggca ggatgcgatc gagcggctgc acattggtgc catacggcac
     4981 gacatgacgc ggctgcgccg aagcgggcga agcgagcgcc gccagcgcca gaaaaactgc
     5041 cggaatcgcg cgcctgaaca tgggcttctc taccgttttc gcaatgaacg gccgatgaac
     5101 gggggctgcc cgatgaaccg gcggacgcgg gcatcccaat cctcgtcgtg gccgggctgt
     5161 ttctcccggc catccatgaa cacggcctca gacgcgtgcc attcacagtt cgttccaccg
     5221 aggacagctc gctcctgagg attggtcacc accacatagc cagaacacgc cgaatcgtga
     5281 cgggatcgac gcgtttgggc cacgccctgg atctgctatg attgggctgt cctggagcgg
     5341 aaaccgtgcc gcggtagctt ctccatcacc gggagcagcc ggatgcgctt cgccgcttta
     5401 gatgctcggc tttggcaatt ccggccgggc cggaaatcgc atcgcgcgac tctgcggtat
     5461 cgccctccca ctgacgccgc atccacatgc ttgagaacga ggaggcttct actcgctggc
     5521 gtcgatcgtt cacacggcca ccaacatcgg cttttcggtc atcggacgcg ggcaggccgt
     5581 ttcgcggctg gtcgttcccc tccactcgag cgaagccccg cttggcgcct tctccgtgca
     5641 gttcatcgat tcggcttcgc agtatctgta ccgggacttt tcggtcgtcg ccagcaaggc
     5701 caccgcgacc aacagcggct gcggcaccgc gatttcatcg accttcgcga ccgggctctc
     5761 gcccgccacg cagcatggcg gggtggtcga gaacctctat gtgggcacgg acggcgtgac
     5821 gacgggcgat ccggacagtg ccgtcgcctg gtcgcatttc aacatcggaa tggacctgac
     5881 gggaacgcac cggccgtttg tcttcaacac gctcttttcc ggctgcaccg ggaagggtgt
     5941 gaagggtaat taaacggact cctcgccgcg ctatctcgcc cagacccatc tggttctcaa
     6001 cgagtgctac gatgcgcagg tcaaggcctg cgggatgtgg cacgcctcga ccgcgatcac
     6061 ccgttcgtcg atgggcggca acgtgcaggg cttttcggtg atagacaccc atatcgaggg
     6121 cgtgaagacg ggcatcttcc atcagcatgg aacgaccacc gcgggcgagg acccggacgg
     6181 caagctgatc ggctgctcgg tgcagtaccg cgacgtgggt ttcgacatcg ccaatgtttc
     6241 gaagtggcgc gtggcgctga acggcttccg caacgagtgg gacgggagcc ccagcgaaac
     6301 cccggtctac gacatgcgct tccgcgctgc gaacgtcgtc tcgcttgtgc agaacacgtt
     6361 cgcagtcggg ggcaatccgg cccgcgtcaa tatgctcatc gatgccgact cgacgatgaa
     6421 ccacgtggcc cacgcgatgc ggtttctcat cgatgcccac cagttcggcg acaacaccag
     6481 cggagagggc gccagcttct tcggcatctc cgccatccag atcaccgcct cggccaccga
     6541 catccagatc ggcgcctcca accagtatct cggcccgttc agcggcgcca tcgtcgacga
     6601 cgcctccggc gccgccgtca cgctggcggg atttcccggc gtgccgggcg atcccaccca
     6661 gccgggcgtc gtggcgccca gcagcgccga tcctgcggca ggaatcacca cgctgggaag
     6721 tggctcaggg ccgtggccag cgagtttcag cctgggacat taccttccca ctaacagttc
     6781 gggaatcaca ccggtgcccc caacaacggg atgggtattc tacaccgatt cgacgggaaa
     6841 gctttacgca caatatcggg acaatggtcc cattctgctt ctttcgccat gatgatcgct
     6901 taaactaacc gaccgaattt ctgggcgatt gagattgtgc ggaagagaca cgcggcggag
     6961 gatttgcgct ggttggcgcg gtgcacgagg gacatgcgcc aagcgcttcg cttgcttgcg
     7021 gtcgcgttgg ttctggatgg ataaggtgaa cccgggacgc ccgccgtgtc tcgacgtggg
     7081 gaaatttcga gagctcaatc ccctctaaaa aacgtctggg agtatctgtg caagaacaag
     7141 cttgccaatc gcatctacgt cgactgcgac gcgatcattt tcgcatactg caaagcccag
     7201 aacgatctga tgtccgtgca agaaaccaac gcctccatcg cgcgccgaaa ctgggcctcg
     7261 cccgcgtgtc atgacttcgg cgtgttcgta ttatttattc gacaaaggtg catcgggagc
     7321 ggtagcacga cgtgaatttt accgaaatca ggtcatgtgc gctgcgccag tgaaaagcaa
     7381 cccggcgatc atctatgtcg taatcgccgg acggtgggct tagctggcaa acaacatgcc
     7441 aaggaggatt gttatccgct cttaattaga aactacggcg tgatctcaaa aaccgagcca
     7501 taaccgtgcc cgccgccttt gctagccgtt ccgtacatgt ttccgctgct gtccgccacc
     7561 agtccgccca caggattcgc gccattatcc cccttcgcga att
//
LOCUS       DASZMC010000355         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_444391,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000355 DASZMC010000000
VERSION     DASZMC010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444391"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VG274_07265"
     CDS             complement(<1..567)
                     /locus_tag="VG274_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWE06490.1"
                     /translation="MELTLQQPRAALPARPLLHLMALAREGDRREGILLRQNKGWFQV
                     SGMGHEALAAIVYAMNAGDYLFPYYRDRAMVLARGVSNYELALAYHAKGESSSGGRQM
                     PGHFSDRPRNIFSGSTPTASQCLPAAGAAWAFQMAGTGQVSVTSVGDASIRQGEFYEA
                     LAFALQEKLPCIFVIEDNHYGISTPTERY"
     gene            897..>2285
                     /locus_tag="VG274_07270"
     CDS             897..>2285
                     /locus_tag="VG274_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWE06491.1"
                     /translation="MSDEARNVLPETTGNIAHARSMSTRPWEDRLVAYRNIGALLAER
                     ARRHPEKNWLTFYDENGRSSCFSYGEFYEAACRTAGVLQDRLGVRAGDRIATLLVNDP
                     RAVLIYFAAWMLGATVVPVNIGEDDSRVAFILRNSEAKAAFALPEQIERIESMRSTLP
                     SLKAAVRVNTGRPLRSPVTPNNGGTGRISDNTISPIIEGRVELDFDTALADVEPLESF
                     PNLSSDTECPIVYTSGTTGAPKGVVLEQYNLLADARGIAEWHRLGPSDRAMCVLPIHH
                     VNGTVVTLMTPLYSGGSVVLNRKFSAQSFWRTLAEEACTWVSVVPTVLAFLCERRDDL
                     RPFDLSRFRHIVCGAGPLTTDLAARFHDCFHIRVVHGYGLSETTCYSCFLPIDLGEKE
                     YLSWMTERGFPSIGCPILANEMAVHDESGNALGEGERGEIVIRGHNVMKHYFQRPDAN
                     RDAFAHGWFRSGD"
BASE COUNT          414 a          734 c          728 g          409 t
ORIGIN      
        1 gtaccgttcg gtgggcgtgc tgatcccata gtggttgtcc tcgatcacga agatgcaggg
       61 cagcttttcc tggagcgcga acgcgagcgc ttcgtagaat tcgccctggc ggatcgacgc
      121 atcgccgacg ctcgtgaccg acacctgacc ggttcccgcc atctggaacg cccaggccgc
      181 ccccgcggcc ggcaggcatt gcgacgcggt gggggtcgag ccggagaaga tgttgcgcgg
      241 gcggtcgctg aagtggccgg gcatctgccg tccgccgctg ctgctttcgc ccttagcgtg
      301 gtacgcgagg gcgagctcgt agttggagac gccccgcgcc agcaccatgg cgcggtctcg
      361 gtagtagggg aacaggtaat cgccggcgtt catcgcatag acgatcgcgg ccagcgcctc
      421 gtgccccatg ccggacacct ggaaccagcc tttattttgc cgcagcagga tgccctcgcg
      481 gcggtcgccc tcgcgggcga gcgccatcag gtgcagcagc ggtcttgcgg gcagtgccgc
      541 ccgcggctgt tgcagagtca gctccattgc cttctcctat cgtaaagtta aacagagacg
      601 ggcgggacgc ccatcccatg atcggacatc gacccgcgtt tcgcgcgagg tctactgaga
      661 ttccattttc ggcaagttca ttacttatat atacggctgc atctaatgaa aatataccgc
      721 gaaaagaagg aacaatatcg aagaagcgga aacgtcaacg ctgggcgccc attcgcagta
      781 ttaaagatat gggcggatac gcaagcgccg ggcttcggcc ctaaccccca atcccggggg
      841 gcatggctgc gcctgacgca gccgaaatag aagtatcgaa attgcgaggc tcaatgatga
      901 gtgacgaagc gcggaatgtg ctgcctgaga ctacaggcaa tatcgcacac gcccggtcga
      961 tgtcgacccg gccgtgggaa gacaggctgg tggcctatcg gaatatcggc gctctgcttg
     1021 cggaaagggc gcggcggcat cccgagaaga actggctgac tttctacgat gaaaacggcc
     1081 ggtcgagctg cttttcctac ggcgagttct acgaagccgc ctgccgcacg gccggcgtgt
     1141 tgcaggaccg gctcggcgtg cgcgccggcg atcggatcgc gacgctgctc gtcaacgatc
     1201 cgcgcgccgt cctcatctac ttcgccgcct ggatgctcgg cgcgaccgtc gtgccggtca
     1261 atatcggcga ggacgacagc cgcgtcgcgt tcatcctgcg caactccgag gcgaaggcgg
     1321 cattcgccct tccggaacag atcgagcgca tcgagtcgat gcgatcgacc ctgccgtcgc
     1381 tgaaggcggc cgtgcgcgtg aacaccgggc ggccgcttag gagccccgtt acccccaata
     1441 atggggggac aggccgtatt agcgacaaca ccatctcccc cattattgag ggccgggtgg
     1501 aactcgattt cgacaccgcc ctcgccgatg tcgagccgct cgaaagcttt ccgaatctct
     1561 cgtcggacac ggagtgcccc atcgtgtaca cgtcgggtac gaccggggcg ccgaaggggg
     1621 tggtgctgga gcagtacaat ctgctcgccg atgcccgcgg tatcgcggag tggcaccggc
     1681 tcgggccaag cgaccgggcg atgtgcgtgc tgccgatcca ccatgtcaac gggacagtcg
     1741 tcacgctgat gacgccgctc tattcgggcg ggagcgtcgt cctgaaccgc aagttcagcg
     1801 cgcaatcgtt ctggcggacg ctggccgagg aagcctgcac ctgggtgagc gtggtgccga
     1861 ccgtgcttgc gttcctttgc gagcgccgcg acgacctccg tccattcgac ctctcgcgct
     1921 tccgccacat cgtctgcggc gcggggccgc tcaccaccga cctcgccgcc cgcttccacg
     1981 actgcttcca tatccgagtc gtccacggct acggcctatc ggagaccacc tgctactcgt
     2041 gcttcctgcc gatcgacctg ggcgagaagg aatatctgtc ctggatgacg gagcgcgggt
     2101 tcccgtcgat cggctgcccg atcctggcga acgaaatggc cgtgcacgac gaatccggca
     2161 acgcgctcgg cgaaggcgag cgcggcgaaa ttgtgattcg cggtcacaac gtcatgaagc
     2221 actacttcca gcgccccgac gccaaccggg acgccttcgc ccacggctgg tttcgctcgg
     2281 gagac
//
LOCUS       DASZMC010000356         6609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_965466,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000356 DASZMC010000000
VERSION     DASZMC010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965466"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            482..1141
                     /locus_tag="VG274_07275"
     CDS             482..1141
                     /locus_tag="VG274_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE06492.1"
                     /translation="MSEFIVHNIPGSPYGRAVLATLAEKNARYRLVPVAPGTTRSEPH
                     ISRHPFGRVPVLEHDGFAIYETQAILRYLDRALPEPALTPADPRAAARMDQVMNVCDW
                     YLFQGVNNVIAFHRIVGPRLMGLTPDEAAIAAAMPKAHVVFKELSRLLADKSYFAARN
                     MSLADIHVATQLDFLAMTPEWKALTTTTPNLVGWLDRMNGRASFRTTTWERVAELAKA
                     A"
     gene            1157..1477
                     /locus_tag="VG274_07280"
     CDS             1157..1477
                     /locus_tag="VG274_07280"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWE06493.1"
                     /translation="MAYANALTALADPTRRRIFERLRKGPRPVGKLAAGLPVSRPAVS
                     QHLKVLKEAGLVQEEREGARHVYSIDPKGLGQLRAWLDQFWDSALDAFRAEVEKTAGK
                     ENSK"
     gene            1480..1941
                     /locus_tag="VG274_07285"
     CDS             1480..1941
                     /locus_tag="VG274_07285"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWE06494.1"
                     /translation="MPTIRAAPVKKSVRVSASQARAFEVFTARFDSWWPKSHHIGKAE
                     MKEAVIEPRAGGRWYEKGVDDSECNWGTVLAWEPPSRVILSWHINGKFQPDEGVQSEV
                     EIRFIPESADATRVELEHRIAAADAQDIRAAVDSPQGWGSLLELFARRATA"
     gene            2168..3565
                     /locus_tag="VG274_07290"
     CDS             2168..3565
                     /locus_tag="VG274_07290"
                     /inference="COORDINATES: protein motif:HMM:NF005682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HWE06495.1"
                     /translation="MARDSTTHNLTGHWQALDAAHHIHPFSDAAALVREGVRVITRAD
                     GVYLWDSEGRRLIDGMSGLWCVQVGYGNRELAEAGCEALKTLPYYNHFFKTTNPWTVE
                     LAAKLAQILPEEHERVLFANSGSEANDSALKLIRYYWNLKGRPEKKIHLSRDYAYHGV
                     TMAAASLSGLSPMHPQWDLPLPGFEKVPAPYWFAAKTAGYGDIGPEEFGLLIAERLEE
                     KIREIGAHRVASFSAEPVQGAGGLIFPPATYWPEVQRICREYDVLLHLDEVITGFGRT
                     GEWFGADTYAIAPDIMTMAKGLSSGYQPISAVSLGSRMAEAIMSANEELVHGFTYSGH
                     PVAAAVALKNLEVLERYGLVPRVKHSIGPYLQRRLRETFESHPIVGEVRGSGMLAAIE
                     LVPDKRVRRFFPRDSDVGTRCRNYCFENGLVMRAIRDTMVLAPPLVASESEIEEIVSR
                     AKDAIDRTARDLGAM"
     gene            3570..3725
                     /locus_tag="VG274_07295"
     CDS             3570..3725
                     /locus_tag="VG274_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06496.1"
                     /translation="MDSILMSVDRGVARPIGKRDGDPVSSANSKSGIAERISDVAHVT
                     NLPPANK"
     gene            complement(4021..4650)
                     /locus_tag="VG274_07300"
     CDS             complement(4021..4650)
                     /locus_tag="VG274_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HWE06497.1"
                     /translation="MPHSLRNLDVSPGVVLWPGCLAPVAQTALIEDVLVRVRDAPFYR
                     PRMPRSGRPFSVEETNLGTLGWYSDEAGYRYETCHPRTRRSWPPMPESLVALWRTLTS
                     HPALPECCLVNLYRGDARMGLHQDRDEAALDAPVLSISLGDDAIFRIGGTTRRLPTRR
                     FTLRSGDAVVFGGVARLAYHGIDRTIANSSSLVPGGGRINLTLRRVSRF"
     gene            complement(4655..5266)
                     /locus_tag="VG274_07305"
     CDS             complement(4655..5266)
                     /locus_tag="VG274_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06498.1"
                     /translation="MILDLPYNFHWIERGEAARSAQAYMGFLGPFLRAHGIRALVNLR
                     GSNPRYFWWRHETRVCAKESVMHRDVRLNSRQLPLPAMLADLLDACDEAPRPFLFKCS
                     GGQDRTSLAAALFVVHRHGWNAFAKAERQFAQWPYLHWPQARQRWLRLLLHFAREQAA
                     GASLRNWIGRDYSAYAFKEWLEARGEGQSFQGLYGVRGNAPGR"
     gene            complement(5543..6490)
                     /locus_tag="VG274_07310"
     CDS             complement(5543..6490)
                     /locus_tag="VG274_07310"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HWE06499.1"
                     /translation="MPIASCLAQASTERGTFSVLFENDYFFHTDRDYTNGVEFAWTTA
                     PSDTPDWSVNLARMLPFFASSGEVRTSYSLGQNIYTPADLTLANPPPSARPYAGWLYL
                     AYGLIEKTDTTLDQLQVQLGMVGPASLAEETQKYVHAVLGDINPQGWGTQLHNEPAFL
                     IGDERAWRVLATGTIGGFSFDVDPHVGGVVGSVYDFVNAGAMARIGFALPDDYGPPRI
                     EPSLPGTNFFEPAESFGWYLFAGVDGRAIARNLFLDGNTWEASRHVDKIPLVGDLQFG
                     AAITFEQWRLSFMHVFRTREYTTQVHADQFGAIDLSYRF"
BASE COUNT         1264 a         2084 c         2075 g         1186 t
ORIGIN      
        1 cgaaattccg cgcctcgggc gacctgcgcg aagcgctggg cgaaaatttc gtgcttctct
       61 acagcgacgt caaagcctcc gagcacgacg cctatcagca ggtgatcagt ccctgggagc
      121 gccagcacct gctgctgaac gtctgagaat cccccgcgcg catgttctct cactgtcacg
      181 gcccgcctgc gggcgtgggc gccaggataa gtggctcgcc gcacgcgaaa gcgtcgttac
      241 cctagcgtgt cattcccgcg aaagcgggaa cccagggcaa agggcgaggc gcttgcccct
      301 ggatgcccgc ttcggcgggc atgacatgtt ggttcaagat gagcgcgatc cgctctggct
      361 ggtttcagct gtgctgataa aattcagtaa tgtaagtgtt gacttacggt aagcgacatc
      421 ttacgtaatc actcgacgag ctgcctcaaa caggtggcca atttcggagg aagaatgagc
      481 catgtccgag ttcatcgtgc acaacatccc tggaagccca tacgggcggg ccgtccttgc
      541 aaccctggcc gagaagaatg cgcgctaccg gctcgtgccc gtcgcacccg gaacaacgcg
      601 gagcgagccg catatttcgc gtcatccgtt cgggcgcgtg ccggttctcg aacatgacgg
      661 tttcgcgatc tatgagacgc aggccattct gcgctatctc gaccgggcgc tgcccgagcc
      721 tgccctgacg cccgccgatc cgagggccgc cgcccggatg gatcaggtga tgaatgtctg
      781 cgattggtac ctgttccagg gcgtgaacaa cgtcatcgcc ttccatcgca tcgtgggacc
      841 gcgtctgatg ggtttgaccc ccgacgaagc ggccatcgcc gcagcgatgc cgaaggcgca
      901 tgtcgtgttc aaggagctgt cgcgtctgct cgccgacaag tcctatttcg cggcgcgcaa
      961 tatgagtctg gcggacattc acgtcgcgac ccaactcgac ttcctcgcca tgacgccgga
     1021 atggaaggcc ctgacgacga ccactcccaa tcttgtcggc tggctcgatc gcatgaatgg
     1081 tcgcgccagt ttcaggacga ccacatggga gcgggtggcg gaactggcga aggcggcgtg
     1141 atccggcgcg gattccatgg cttatgcaaa tgccctgacg gccctggccg accccacgcg
     1201 gcgccgtatc ttcgagcggc tgcgcaaggg tcccaggccc gtgggcaagc ttgcggccgg
     1261 acttcccgtc agccgcccgg ccgtgtcgca gcatctgaag gtgctcaaag aggccgggct
     1321 ggtgcaggaa gaacgcgaag gcgcacgcca tgtctacagt atcgacccga aggggctggg
     1381 ccagcttcgc gcctggctcg atcagttctg ggattcggcg ctcgacgcct tccgcgccga
     1441 agtcgagaaa acagccggca aggagaactc caagtgacaa tgcctacaat ccgcgccgcg
     1501 ccggtgaaga agagcgttcg cgtcagcgcg tcccaggctc gcgccttcga ggtgttcacg
     1561 gcacgcttcg attcgtggtg gccaaaatcg catcacatcg gcaaggccga aatgaaggaa
     1621 gccgtaatcg agccgcgggc aggtggacgc tggtacgaaa agggcgtgga tgattccgaa
     1681 tgcaactggg gcacggtgct ggcgtgggag ccgccatcgc gggtgattct ctcctggcac
     1741 atcaacggga aattccagcc cgatgaaggc gtccagagcg aggtcgagat acggttcatt
     1801 cccgaaagcg ccgatgcgac gcgcgtcgaa ctcgagcacc gaatcgcggc cgccgatgca
     1861 caggacattc gcgcggcggt cgattcgccg cagggctggg gatcgctgct tgaactgttt
     1921 gcccggcgcg ccacggcctg acatcgggtt tggtatcgtc ccgccggcgc accggtttca
     1981 cggagaattg cttctcaccg cgcggcggcg ctgctagagt gggcctgtga acgtcaaacg
     2041 cgccttgttg attctctgcg cgcgcatcgg attcaatcca tagtccggcg cgcatgcgcg
     2101 cgcaggtggc cgggtcagat ccggccatcg cgcgcgaact tcttcctttc gcacgcagag
     2161 aacggtcatg gcgcgggaca gcacaactca caatctcacc ggacattggc aggcgctcga
     2221 cgccgcgcac cacattcatc ctttttcgga tgcggctgcg ctcgttcgcg aaggcgtgcg
     2281 cgtcatcacc agggccgacg gcgtctacct ttgggattcc gagggcaggc gcctcatcga
     2341 tggaatgtcc ggactatggt gcgtgcaggt gggctacgga aaccgcgaac tggccgaggc
     2401 cggttgcgag gcgctgaaga cgcttcccta ctacaatcat tttttcaaga cgaccaatcc
     2461 ctggaccgtc gaactggcgg caaaactcgc gcagatcctg ccggaggaac atgagcgcgt
     2521 gctgttcgca aactcgggct ccgaagccaa tgattcggcg ctcaagctca tccgctacta
     2581 ctggaacctc aaaggccggc cggaaaagaa gattcacctg tcgcgcgact acgcctatca
     2641 cggcgtgacg atggcagcgg cttcgctgtc ggggctttcg ccgatgcatc cgcaatggga
     2701 tctgcctttg ccgggcttcg agaaagtgcc ggcgccctat tggttcgcag ccaagacggc
     2761 cggctacggc gacatcgggc cagaggaatt cgggctgttg atcgccgagc gcctggagga
     2821 gaagatccgc gaaatcggcg ctcaccgcgt cgcgtcgttc tccgcggaac cagtccaggg
     2881 cgccggcggc ctgattttcc cacccgccac ctactggccg gaagtgcagc gaatctgccg
     2941 cgagtacgac gtgctcctgc atctcgacga agtgatcacc ggctttggcc ggaccggcga
     3001 atggtttggc gccgacacct atgcgatcgc ccccgacatc atgacgatgg caaaggggct
     3061 ttcgtcgggc taccagccga tttcggccgt atcgctcggt tcgcggatgg cggaagcgat
     3121 catgtccgcc aacgaagagc tcgtccacgg cttcacctat tcggggcatc cggtggccgc
     3181 cgcggttgcg ctgaagaatc tcgaagtgct ggaacgatac ggcctggttc cccgcgtgaa
     3241 acactcgatc ggcccctacc tgcagaggcg gcttcgcgag actttcgaat cccatccgat
     3301 cgtgggcgag gtgcggggaa gcggcatgct ggcggcgatc gaactggtgc ccgacaagcg
     3361 cgtccgcaga ttcttcccgc gcgattcgga cgtgggcacg cggtgccgca attattgctt
     3421 cgaaaatggc ctggtcatgc gcgccatccg cgacacgatg gttctcgcgc cacctcttgt
     3481 cgccagcgag tcggagatcg aggaaatcgt ctcccgggcg aaagatgcga tcgaccgcac
     3541 cgcccgggat ctcggcgcga tgtgacgcca tggattcgat cctcatgtcg gtcgatcgcg
     3601 gcgtcgcccg cccgatcggc aagcgagacg gcgatccggt cagttcggcg aactccaagt
     3661 cggggattgc cgaacggatc tccgacgtgg ctcacgtcac gaatttgccg ccggcaaaca
     3721 aatgatccac atctccattt tgtgatactt atggcactgt cacgcacaaa agagctgtca
     3781 gaattatcac gaaaaaaata gttgcaaact gtggcatcag tgcatagctt tgacctcgga
     3841 aggacgattt accgtccggc aggcactgcc cccccgctcg cacgccggat ggggtccttc
     3901 catcggcgcc ggtcatcccc tcccggcgtc agcgcgtaac ggcgcgtgtg acgcccgatc
     3961 agccccctgg tcgggcgtct tcattttcgg ggcatctttt ccttcagtgc attttctcat
     4021 tcagaaccgc gatacgcggc gaagcgtgag attgatgcgc ccgccacccg gcaccagcga
     4081 agacgaattc gcgatcgtgc ggtcgatgcc gtgataggcg agccgcgcga cgccgccgaa
     4141 aacaaccgcg tcgcccgacc tcagcgtgaa ccggcgcgtc ggcaaccggc gcgtcgtgcc
     4201 gccgatgcga aagatcgcgt cgtcgccgag cgagatcgac agcaccggcg catcgagcgc
     4261 cgcctcgtcg cgatcctgat gcaaccccat gcgggcgtcg ccgcgataga gattgaccag
     4321 acagcattcc ggcagcgcgg gatggctggt caaagttctc cagagcgcga ctaggctttc
     4381 cggcattggc ggccacgagc gtcgcgttcg cggatgacag gtttcgtagc gatagcccgc
     4441 ttcatccgag taccagccga gagttccgag attggtctcc tcgacgctga atggcctgcc
     4501 cgagcgcggc atgcgcggcc ggtagaatgg cgcgtcgcga acgcgaacga gcacgtcctc
     4561 gatcagcgcg gtctgcgcga ccggcgccag acagcccggc cagagcacga cgcccggaga
     4621 cacatccagg tttcgaaggg aatgcggcac ggcatcatcg ccccggagca tttccccgca
     4681 cgccataaag gccctggaac gattgaccct ccccccgcgc ctcgagccat tccttgaatg
     4741 catacgcgga ataatccctc ccgatccagt tccgcagcga ggcgcctgcc gcctgttcgc
     4801 gcgcgaaatg cagaaggagc ctgagccagc gctgccgcgc ttgcggccag tgcagatagg
     4861 gccattgggc gaactgccgt tcggcctttg caaacgcgtt ccagccgtgg cgatgcacaa
     4921 cgaaaagcgc cgcagcgagg ctggtccgat cctggcctcc cgaacatttg aacaggaacg
     4981 ggcgtggcgc ctcgtcacaa gcgtcgagaa ggtctgcaag catcgccggc aggggaagct
     5041 ggcgcgaatt gagcctcacg tctcgatgca tgacgctctc tttcgcgcag acgcgcgtct
     5101 catgccgcca ccagaaatag cgcggattgg atcctctcag attgacgagc gcccggatgc
     5161 cgtgggcgcg caggaacggt cccagaaatc ccatataggc ctgcgcggag cgcgccgcct
     5221 cgccccgctc gatccagtgg aaattgtagg gaaggtcgag aatcaagtgg cgaggactgt
     5281 ccgaaccgcg ttgctacagt ctcatgcgtg gaccgcacgc gccagacccg tctcgccgcg
     5341 cgccgcgccg gtatcacctt ttgatcttta gcgactcgtg tgtcggcaga cttttgacgc
     5401 agagtacgca gtggatgcgc ggaggacgca gggtatatga gccgcgcgcc tgcggcgcgc
     5461 caaatctttt ccgcgtccac tgcgctgcct ccgcgccctc tgcgttaaat ctgtctttgt
     5521 gtcactcgtc atattccagg tactagaagc gataggaaag gtcgatggcg ccgaactggt
     5581 cggcgtgcac ctgcgtcgtg tattcgcgcg tgcggaacac atgcatgaag gaaagccgcc
     5641 attgctcgaa ggtgatcgcg gcgccgaact gcaggtcgcc gaccagcgga atcttgtcga
     5701 catggcggct cgcttcccag gtgttgccgt cgagaaacag attgcgggcg atcgcccggc
     5761 cgtcgacgcc cgcgaacaga taccagccga acgattccgc cggttcgaag aagttcgttc
     5821 ccggaaggct gggctcgatg cgcggcggac cgtaatcgtc gggaagggcg aagccgatgc
     5881 gcgccatcgc gccagcgttg acgaaatcat acacgctgcc aaccacgccg cccacatgcg
     5941 gatccacatc gaacgagaat ccgccgatgg taccggttgc cagcacccgc catgcccgct
     6001 cgtcgccgat cagaaacgcc ggttcgttat gcaattgggt tccccagcct tgcgggttga
     6061 tgtcgcccag aaccgcatgg acgtatttct gcgtttcttc ggcaagcgag gcgggaccca
     6121 ccatgccaag ctgcacctgc agctgatcga gggtggtgtc ggttttctcg atcaggccgt
     6181 aggcgagata gagccagccc gcatagggac gcgccgaggg tggcgggttg gcgagagtaa
     6241 ggtcggcggg ggtgtagatg ttctgcccga gagaatagct cgtgcgcacc tcgccgctcg
     6301 acgcgaaaaa cggcagcatc cgggccagat tgaccgacca gtccggcgta tcggagggtg
     6361 ccgtggtcca cgcaaactcc acgccgttcg tgtaatcccg gtcggtgtga aagaaataat
     6421 cattctcgaa cagcacgctg aaggtgccgc gttcggtgct ggcctgtgcg aggcaggatg
     6481 cgatcggcaa acacaccgcg cccagagtcg caacacatgc gccaagaatt cgtattcgca
     6541 aacgctggca cccctctcct caggggagcg aacgcggcca tgtcgccttc ggttccggcg
     6601 gagggggcg
//
LOCUS       DASZMC010000357         6974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1275739,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000357 DASZMC010000000
VERSION     DASZMC010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275739"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VG274_07315"
     CDS             <1..901
                     /locus_tag="VG274_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE06500.1"
                     /translation="NPLAETSARLLASGGKVGQSSRGMRLLPHDPAEYLRRLGEAGEG
                     PCDIAGAALMLSALDHAGRDLDPYCAHLAEMAERARLEAHLAPHAEDGARALAALLAG
                     RYGYDGDRLAYDDPQNADFMAVIDRRRGMPVALGILYVHAARAAGFEAAGLNSPGHFL
                     LRIGLRDSEALIDPFNGGAAIEREPLDTPPSMRAVAADEKEALRPQRDVEVLLRLENN
                     LKLRALQAGEHVRALEIARRMVLIAPRHPDLWIDLARLNETAGVLGAAQKAYEACLTL
                     ARPGAPLHNEAALGLQGLKRRLN"
     gene            914..1864
                     /gene="gshB"
                     /locus_tag="VG274_07320"
     CDS             914..1864
                     /gene="gshB"
                     /locus_tag="VG274_07320"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HWE06501.1"
                     /translation="MALTVAIQMDPIERIDIAGDSTFALALEAQNRGHGLVYYGPRDL
                     SFRDGVVSARVRALTVRNERGNHFTLGPANLWSLGRVQVVLMRQDPPFDMAYITATHL
                     LERLAPGTLVVNDPFHVRNAPEKLFVTLFGNFMPPTLITSDREEIRRFRAEHRDLILK
                     PLYGNGGTGVFRVKEDDENLGALLEMFTQLYREPVIVQRYVPEVRKGDKRIILVDGVF
                     AGAINRVPAPGEARSNLHVGGRPEPATLSEQETRMCDALGPELQKRGLIFAGIDVIGG
                     YLTEVNVTSPTGIQEIRRFGGADIAKLIWDAIERRLERGR"
     gene            complement(1874..2725)
                     /locus_tag="VG274_07325"
     CDS             complement(1874..2725)
                     /locus_tag="VG274_07325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HWE06502.1"
                     /translation="MAPRVVNVHEPGAFEVVLEQWPVLATSGSLEVRLAETESEVEAA
                     QRLRYRVFYEEMSAIPSPAMREHRRDFDRFDDYCDHVFVVDRATTEEDGQPAVVGTYR
                     LMRDLDARRAGGFYTSGEYNISNLLAAAPAGARFLELGRSCVLKSYRARPATLQLLWR
                     GVMVYLTRFSIDTLFGCASLHGTDADSLALPLSYLHHYHAMQSTPVRARSELYVPMNR
                     MLKQAIDVREARRTLPPLVKGYLRAGAEIGDGAVIDRQFGTTDVFIHMPVAKIDLRYQ
                     HRFAAVR"
     gene            complement(2847..4178)
                     /locus_tag="VG274_07330"
     CDS             complement(2847..4178)
                     /locus_tag="VG274_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE06503.1"
                     /translation="MTVSARSRASFRSYLEPRVLVMLALGFSSGLPFLLTGNTFGYWL
                     RDEGTSLTAIGFISWVGIAYSLKFLWAPLIDRVNLPLLGRLGRRRGWMLLAQAAVASG
                     LFAMAAAGRGAGLLVLGAFALLVAFASATQDIVIDAWRIESAESGEELGLLSSAYQFG
                     YRLALLATDALILIAAAHIGWRVSYSACGFAMIAGIGATLIALEPARADSVIEQKTRE
                     APLSSLRGLFDAIAGPFTVFFRTHGWLALLMLVAIGLYRLPDFVMGPMANPFYHDIGL
                     SKDYVGAVRGTVGVIAALLGIAAGGACALRLGYMRALIIGGLLQALAIAAFATLARAN
                     PGTLLFATIMSGDNFSTGFAGVALVSYMSSLTSLGYTATQYALLSSTYAWAGKILKGF
                     SGVIVEALVANHGLMAAYAIFFIGAGAIGIPAIILFGILAARQGAPAHALT"
     gene            complement(4175..6394)
                     /locus_tag="VG274_07335"
     CDS             complement(4175..6394)
                     /locus_tag="VG274_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE06504.1"
                     /translation="MPPWRTAKLVLGACLVAVAAMSAADTAIHGGLTSSSPWLGGAVA
                     AIAMLGMVLALLDARDGRLALEARVSGLRRFIAELEQALSAVSAMNVQARENEIRFKG
                     LVDAQEDSVFRRTPDGRLTYGNNAFFRLFGLDPKDDLGNPFTPESRCDSGTPVFGSFD
                     GRGNGGTRTRYDQNLRTVYGWRWIAWEEYAVRDATGRLIEVQSVGRDITERKALEDAL
                     TEARDRAEAANRAKSGFLAAMSHEIRTPMNGVLGMARLLLETRMSPEQRSYAEAIRQS
                     GEALLSLIEEILDFSRIESGTIALDQDEVDIRRLTESVVELLAPRAHGKAIEVAAVIA
                     PDTPALVRADGMRLRQILTNLVGNAVKFTERGGVRVDVRPVQDFARRVLRFEIRDTGV
                     GVPARKRHDIFQEFVQADSTHARKFGGSGLGLAICKRLVEAMDGAIGVEAAPDGGSIF
                     WFTVPAPILCKAAPGDGLRLSHTRAAIVTANAMLHDSLTAQIESAGGEVVPLASAGAN
                     ENTPPVAIDAVLIDAGTSSEVELAALPGGISRSIVILTPAARSKLEELRNAGFSSYLV
                     KPIRQASLVSRILAEPEAPAVDHAPQGEFPPPDAAAAQSAGRALRILVAEDNPINALL
                     TRELLRRRGHLVTEVTSGEDAVAALRAERFDILLTDLHMPGLDGIETARRIRATEFAL
                     SLPPTPIVAITADAVDTGRQACQDAGMDGFITKPIAPAELDAMIARLFPDGVREAAE"
     gene            6659..>6974
                     /locus_tag="VG274_07340"
     CDS             6659..>6974
                     /locus_tag="VG274_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWE06505.1"
                     /translation="MNSNLHRLAGFLAGMLLACASNGALAADISGAGATFPYPVYAKW
                     AEAYRAKTGVGLNYQSIGSGGGIAQIKAKTVTFGASDAPLKPADVESSGLVQFPTVVG
                     GDV"
BASE COUNT         1392 a         2221 c         2151 g         1210 t
ORIGIN      
        1 gaacccgctg gccgaaacat ctgcgcgact cttggcgtcc gggggcaagg tagggcaatc
       61 ttcgcgcggc atgcgcctgt tgccccatga tcccgccgaa tatctgcgtc ggctcggtga
      121 agctggcgaa ggaccttgcg acatcgcggg cgcggcgctg atgctgtcgg cgcttgacca
      181 tgccgggcgc gatctcgatc cgtattgcgc gcatctcgcg gagatggccg aacgggcgcg
      241 tctggaagca cacctcgccc cgcacgccga agacggagcg cgcgcgctcg ccgcgctcct
      301 cgccggccgc tatggatatg acggcgaccg gcttgcctac gatgatccgc agaatgccga
      361 tttcatggcc gtgatcgatc ggcgcagggg aatgccggtt gctctgggga tactctacgt
      421 gcacgcggcg cgcgccgccg gattcgaagc tgcaggactg aattcgcccg gacattttct
      481 tctgcggatc ggcttgcgcg acagcgaggc cctgatcgat cccttcaatg gcggcgccgc
      541 gatcgagcgc gagccgctcg acacgccgcc ttccatgcgc gcggtcgccg ccgacgaaaa
      601 agaggcgctg cggcctcaga gagacgtcga ggtgctgctc cgtctcgaaa acaatctcaa
      661 acttcgcgcg ctgcaggccg gcgagcacgt gcgcgcgctg gagatcgcca ggcggatggt
      721 cctcattgcg ccccgccatc cggatctgtg gatagacctt gcccgcctga atgaaaccgc
      781 gggcgtgctt ggcgccgcac agaaggccta tgaggcatgc ctgacattgg cccggcccgg
      841 agcgccgctt cacaacgagg ccgccctcgg gttacagggc ctcaaacgcc gcctgaatta
      901 ggagccgcag agcttggcgc tcacagtcgc aattcagatg gaccccatcg agcggatcga
      961 catcgcggga gattcgacgt tcgcgctggc gctggaagcg cagaaccgcg gacacgggct
     1021 ggtgtactac ggaccgcgcg acctttcgtt tcgggacggt gtcgtcagcg cgcgcgtgag
     1081 agcgctcacg gtacgcaacg aacgcggcaa tcacttcact ctcggtcccg ccaatctctg
     1141 gagcctcggg cgggtgcagg tcgtgctcat gcgacaggac ccgccattcg acatggccta
     1201 tatcacggcc acacacttgc ttgagcgact tgcgcccgga actcttgtgg tcaatgaccc
     1261 cttccacgtc agaaacgcac cggaaaaact gtttgttaca ctgttcggga atttcatgcc
     1321 gcccacgctc attaccagcg accgcgagga gattcgccgt ttccgcgcgg agcatcgcga
     1381 tctgatattg aagcccctct acggcaatgg cggaaccggc gtatttcgcg tcaaggagga
     1441 tgacgaaaat ctcggcgcgc tgctggagat gttcacgcag ctgtatcgcg agccggtcat
     1501 cgtccaacga tacgttccgg aagtccgaaa aggcgacaaa cgaatcattc tggtggacgg
     1561 cgtgttcgca ggcgccatca accgcgttcc ggcgccaggc gaagctcgat cgaacctgca
     1621 tgtcggaggc aggcccgagc ccgcaacgct gagcgagcag gaaacgcgga tgtgcgacgc
     1681 gcttggcccc gaattgcaga agagaggcct catcttcgcg ggaatcgacg ttattggagg
     1741 gtatctgacg gaagtgaacg tcacgtcccc gaccggtatc caggaaatcc ggcgcttcgg
     1801 cggcgccgat attgcgaagc tgatttggga tgcgatcgag cgccggctcg agcggggccg
     1861 atgagaaagc agcctagcgc accgcggcaa aacggtgctg gtagcgcaaa tcgattttag
     1921 ccacgggcat atggatgaat acgtcagtcg tgccgaattg tctgtcgatg acggcgccgt
     1981 cgccgatctc cgcgcccgct ctcagatatc ccttgaccag gggcggcagg gttctgcggg
     2041 cttcgcgcac gtcaatcgct tgcttgagca tgcggttcat cggcacgtac aattcgctgc
     2101 gggcgcgcac cggcgtcgat tgcatcgcgt gataatggtg cagataggaa aggggaagcg
     2161 ccaatgaatc ggcatcggtt ccgtgaagag atgcgcaacc gaacaaagta tctatcgaaa
     2221 accgcgtgag gtataccatg acgccgcgcc acaagagctg cagcgtcgcc ggcctcgcgc
     2281 ggtagctctt gagcacgcag gaacgtccga gttccaggaa ccgcgccccg gccggggcgg
     2341 ccgcgagcag attcgaaatg ttgtattccc cggacgtgta gaagccgccg gcgcgccgcg
     2401 cgtccagatc ccgcatgagg cggtaggttc cgacaaccgc gggctggcca tcctcctccg
     2461 tggtcgcacg gtccacgacg aaaacgtggt cgcaatagtc atcgaaacga tcgaagtcgc
     2521 gccgatgttc gcgcatcgcg ggacttggga tggcggacat ctcctcgtag aaaacccgat
     2581 agcgcaacct ttgcgccgcc tcgacttcag actcggtttc cgccagccgg acttccaggg
     2641 atccggatgt tgcgagaacc ggccattgtt cgaggacgac ttcaaatgca cccggttcgt
     2701 gtacgttgac cactctcggc gccaccttta cacgcagcgt tcgccagcgc ggcgaatgcc
     2761 cctgctgtcg ggtccaatcg tacagacgcg ctcgctttcg tgcaaacggc ccgaattcac
     2821 gaaggaaagc gcatcggcgg cgcctcctaa gtgagcgcgt gcgcaggcgc gccctggcgc
     2881 gccgccaaaa tcccaaatag aatgatcgcc ggaatgccga tcgcccccgc cccgataaaa
     2941 aatatcgcgt aagccgccat cagcccgtga ttcgcaacca gggcttccac aatcacgccg
     3001 gagaatccct tcagaatttt ccccgcccag gcgtaagtcg aacttaacag cgcatactgc
     3061 gtcgcagtat agccaagact cgtcaggctg gacatgtagg aaacgagcgc cacgcccgca
     3121 aagcccgtgc tgaaattgtc gccgctcatg attgtcgcga agagaagcgt acccggattg
     3181 gcgcgcgcga gagtggcaaa ggctgcgatc gcaagcgcct gcagaaggcc gccgatgatg
     3241 agggcacgca tatacccgag ccgcagcgca caggcaccgc cggcggcgat cccgagaagc
     3301 gcggcgatca cgccgaccgt accgcgcacc gctcccacat agtccttaga caggccgata
     3361 tcgtgataga aggggttggc catcggcccc atgacgaaat cgggcaggcg gtaaagaccg
     3421 atcgcgacaa gcatcaaaag cgccagccat ccgtgcgtcc ggaaaaagac ggtgaatggc
     3481 ccggcaatgg cgtcgaagag cccgcgcaac gatgagagag gtgcttcgcg tgttttttgt
     3541 tcaatgacgg aatccgcacg cgcaggctcc agtgcaatca gtgtggctcc gattccagcg
     3601 atcattgcga aaccgcaggc agagtaggaa acgcgccagc cgatatgcgc cgccgcaatc
     3661 aggatgaggg cgtcggtcgc aagcagcgcc aatcggtagc cgaactgata tgccgacgac
     3721 agaagcccga gctcttcgcc gctttcggca ctctcgattc gccaggcgtc gatcacgatg
     3781 tcctgggtcg cggaagcgaa tgcgaccagc agcgcgaatg cgcccaggac cagcagcccg
     3841 gcgcctcgtc ctgccgcagc catcgcgaag agccccgacg ccacggcggc ctgcgcgaga
     3901 agcatccacc cgcgccggcg gcccagccgg ccgagaagcg gaagatttac acgatcaatc
     3961 agcggcgccc aaaggaactt caatgaatag gcgatgccga cccaggagat gaagccgatt
     4021 gcggtcagcg atgtgccttc gtcccgcagc cagtagccaa atgtattgcc cgtgagcaga
     4081 aacgggagcc ccgacgagaa gccgagcgcg agcatgacaa gcacgcgagg ctccagatag
     4141 gatctgaaag acgcgcggct gcgcgcggaa accgtcattc ggcggcttcg cgcacgccgt
     4201 ccggaaacag tctcgcaatc atggcatcga gctccgccgg agcgatcggc ttggtaatga
     4261 aaccatccat gccggcgtcc tggcaagcct gacgcccggt gtccacggcg tccgccgtga
     4321 tcgcgacgat aggcgttggc ggcagggaca gggcgaattc ggttgcccgg atgcgtcgcg
     4381 cggtttcgat gccgtccagt ccgggcatat gaaggtccgt aagcaggatg tcgaaccgct
     4441 ccgctcgcag ggctgctacg gcgtcttcgc ccgacgtcac ctcggtgacg agatgcccgc
     4501 gccgccgcag caactcgcgc gtcaacaatg cattgatcgg gttgtcctcg gcaacaagga
     4561 tgcgaagtgc gcggcccgcg ctctgggcgg cggccgcatc gggtggcggg aattcgccct
     4621 gtggcgcgtg atcgactgcc ggcgcttccg gttctgccaa tatgcgactg accagcgatg
     4681 cctgccggat cggcttcacg agatacgacg agaagcccgc gttcctcaac tcctccagtt
     4741 tgctgcgcgc ggccggcgtc aggatcacga tcgagcggct gatgccccca ggcagagccg
     4801 ccaattcgac ttcgcttgat gttccggcgt cgatgagaac cgcgtcgatg gcaacggggg
     4861 gtgtgttctc attggcgccc gcggacgcga gaggcacaac ttcgccgccg gcagactcga
     4921 tctgcgccgt aaggctgtcg tgcagcattg cattggcggt gacaatcgcg gcacgagtgt
     4981 gggaaagccg caagccgtct cccggcgccg ccttgcacaa aatgggcgcc ggcacggtga
     5041 accagaatat gcttccgcca tcgggggcgg cctccacgcc aatggcgccg tccatcgctt
     5101 cgaccagcct cttgcagatg gcaagcccga gacccgaacc tccgaacttg cgtgcgtgcg
     5161 tggagtccgc ctgcacgaac tcctgaaaaa tgtcgtgccg cttcctcgcg ggcacaccaa
     5221 cccccgtgtc gcgtatctca aagcgcagga cgcgtctggc gaaatcctgg acaggtcgca
     5281 cgtccacccg aactccaccc cgttccgtaa acttgacggc gttgccgacc agattcgtca
     5341 gtatttgtct cagtctcatg ccgtctgcgc ggaccagggc aggggtgtcc ggcgcgatca
     5401 cggcggcaac ttcgattgcc ttgccgtggg cacgaggcgc cagcaattca accacggact
     5461 cggtgaggcg gcggatatcg acctcgtcct gatcaagagc aatggttccc gattcgatcc
     5521 tggaaaaatc gagtatctct tcgatcagcg acagcagggc ttctccagac tgccgaattg
     5581 cttcggcata gcttctttgc tctggcgaca ttcttgtttc caggagcaat ctcgccattc
     5641 cgagcacgcc attcatgggc gtgcgaattt cgtgactcat cgccgcgaga aatcccgact
     5701 tggcgcggtt tgctgcttcc gcccggtcgc gtgcctcggt cagcgcgtct tcaagagcct
     5761 ttcgctccgt aatatcgcgt ccgacgcttt gaacctcgat caatcgtccg gttgcatcgc
     5821 gaactgcata ttcttcccat gcaatccacc gccagccata aaccgtgcgc aaattctgat
     5881 cgtagcgcgt tcgcgtgccg ccgttgccgc gcccgtcaaa gcttccaaac acaggtgttc
     5941 cgctgtcaca acgagactcc ggcgtgaaag gattgccgag gtcgtcttta ggatcgagtc
     6001 cgaacagccg gaagaaagca ttgttgccat aggtcagccg accgtcgggc gtgcggcgaa
     6061 agaccgaatc ttcctgcgcg tcaaccagcc ccttgaagcg aatttcattc tcgcgcgctt
     6121 gcacgttcat ggccgacacg gcgctcaagg cctgctcgag ctccgcgatg aagcgccgga
     6181 gaccggacac gcgtgcttcg agcgcgaggc ggccgtcgcg tgcgtccagc aacgcgagaa
     6241 ccattccgag catggcgatg gccgcaaccg caccgccaag ccatggcgat gacgacgtca
     6301 acccgccatg gatggcggta tccgccgccg acattgcggc gacggcgacg aggcacgcgc
     6361 caagaacgag cttggcggta cgccagggcg gcatgcggag ctctcccagt cccgcctgga
     6421 ttgaaatatc atccgcattg ccaaaggctt aacccggatc tcacacggaa cggccggttg
     6481 ccccgctcac cggcggcgca acaagggggt atgtcataaa agcttcgcaa aactgtgatg
     6541 gacgttgcac gttagcgcga gcttatcgcg ccaagcgtcc acagggggaa tcaggctggg
     6601 aaacagcctg tttgcgcccg tttgcgattc gccgctggac aagccgagag gggactccat
     6661 gaattcgaac ttgcatcgtc tggcaggctt cctggccgga atgctactgg cctgcgcctc
     6721 gaacggtgcg cttgccgcag atatttcagg tgccggcgca acctttcctt acccggtcta
     6781 cgcaaaatgg gccgaagcct accgggcgaa gaccggcgtc ggcctcaatt accaatcgat
     6841 cgggtcaggc gggggcatcg cgcaaatcaa agccaagacc gtcaccttcg gtgcgagcga
     6901 cgcgcctctc aaacctgccg acgtcgagtc gtccggcctc gtccaattcc cgaccgtcgt
     6961 gggcggcgat gtgc
//
LOCUS       DASZMC010000358         4540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1797599,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000358 DASZMC010000000
VERSION     DASZMC010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797599"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1403)
                     /gene="hpnE"
                     /locus_tag="VG274_07345"
     CDS             complement(147..1403)
                     /gene="hpnE"
                     /locus_tag="VG274_07345"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HWE06506.1"
                     /translation="MINGTVYVIGAGLAGLSAAVELAGRGMRVEIIETAPQPGGRCRS
                     WFDAQIGEVIDNGNHLVLSGNHATFSYLSAIGAGDRLNGPDEARFIFCDVRSGERWTI
                     APNAGAVPWWIFSGNRRVPGTGALDYAKIARLLIANRGRRVADVITCAGPLWERLMRP
                     LLLAALNSAPEEASADLASAILRETLARGGRHCLPRIATPNLAAAFIEPALGFLAGKS
                     ARVRLGERVRSLDFGNSHATGLDLGDQRIDLSTDDRVILAVPSWIATSLVPYIDAPDQ
                     SSAILNAHFRANAGSDAPGMIGVIGGTAEWVFAFPRRISVTVSGADAIVDRERAEIAE
                     LLWRDVARVHGLPMNLPAWQVVKEKRATFRATPEQDARRAPAQTRWQNLFLAGDWTAT
                     GLPATIEGAIRSGHKAARLAAGESVT"
     gene            complement(1400..2239)
                     /gene="hpnD"
                     /locus_tag="VG274_07350"
     CDS             complement(1400..2239)
                     /gene="hpnD"
                     /locus_tag="VG274_07350"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HWE06507.1"
                     /translation="MAIETEARATQQKASGSSFYAAMRLMPKAEREAMYAIYAFCRAV
                     DDIADNGGDPREIRSARLDAWRRDVEALYAGSAAQRAAFLEAVVRRYRPRKEDFLAVI
                     DGMAMDVAEDICAPDAATLDLYCERVASAVGRLSIKIFGMDEEPGFRLAHHLGRALQM
                     TNILRDLDEDAGLGRLYLPRELLDQAAIAIDNPDKVVASPAIDAPCRGVARIARQHYR
                     EADAIIASQPRGDLRAPKLMSAVYSSILDAMERQGWAPPRSRVRIAKTRLLWFFLRYG
                     IPA"
     gene            complement(2229..3125)
                     /gene="hpnC"
                     /locus_tag="VG274_07355"
     CDS             complement(2229..3125)
                     /gene="hpnC"
                     /locus_tag="VG274_07355"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HWE06508.1"
                     /translation="MSEPSFSSGKGHRDENFPVASFLIRKRHRPIILAFYRFARAADD
                     IADHPCAEPAEKLHLLENMWRTLHGEADLSAEAARLRDILAGENLSPQHALQLLEAFR
                     RDVTKLRYANWDELMEYCRYSAMPVGRFVLEVHRESRAAWPASDALCAALQVINHLQD
                     CGKDFRNLDRVYIPLDVLEERGLEPAALGAARSPPALQQVIAQIAQRNSSLLAQSRPF
                     ASQIADHRLALEVALIQRLAEDLNRRLLTQDPLSERVHHNMLETTGCALVAIAEFLVT
                     RRRAKASRLALSSHPHETAHGD"
     gene            complement(3132..4277)
                     /locus_tag="VG274_07360"
     CDS             complement(3132..4277)
                     /locus_tag="VG274_07360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWE06509.1"
                     /translation="MTGVALVPLLVWTYLLLARGFFWLMRERDDRGGSLEIQQSWPSV
                     VAIVPARDEADVIVQTVASLRAQDYPGSFRIVLIDDQSTDGTAEEAVRAARMNGGSES
                     FELLRGQSRPAGWTGKVWAMHQGFRRVSETGAPDYLLFTDADIAHAPDNLRRLVRRAE
                     SGRLVLVSLMAKLHCESFAERLLVPAFVFFFAMIFPFAWANDRTRTTAAAAGGCMLVR
                     RDALVAAGGLEPIAGAIIDDCALARILKQQGPAWLGLTNRATSLRSYSAFADIGRMVA
                     RSAYAQLRYSPALLCGTVLGMLTVYAAPPLLALFAQGNARVEGALAWLLMAAAYQPIL
                     KFYRLTPVWGAALPAIGAIYAGFTIDSAVQHWRGRGGMWKGRAQAPA"
     gene            complement(4274..>4540)
                     /locus_tag="VG274_07365"
     CDS             complement(4274..>4540)
                     /locus_tag="VG274_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06510.1"
                     /translation="TIAELVGRKAPRFELPRTPLFPLAFAAEAVAGLTGKEPFLTRDA
                     LRMAGHHMFFTSVRTERELGYRARPYREALNEAIAWYRGAGYLR"
BASE COUNT          892 a         1467 c         1414 g          767 t
ORIGIN      
        1 cacatattcc gccgggatcg tggcgtcggc ttcgagctcg aacacgaaat gcccatcgtc
       61 acgctgcatc gcaagcagcg ccgtggtcgc gcgtgcgatc gtttgatcga gcgtgggcgc
      121 gctttcccga cgaattccga cagcgctcat gtcaccgatt caccggccgc caatcgcgcc
      181 gccttgtgcc ccgagcggat tgcgccctcg atcgtggccg gaaggcccgt cgctgtccag
      241 tcgccggcca gaaacagatt ttgccatcgc gtttgtgctg gggctcgccg cgcatcctgt
      301 tccggcgtgg cgcgaaacgt ggcgcgtttc tccttgacaa cctgccaggc cggcaaattc
      361 atcggaaggc cgtgcacacg cgcgacgtcg cgccagagaa gttctgcaat ctcggcccgc
      421 tcccgatcga cgatggcgtc cgcgccgctc actgtcaccg agatccgccg gggaaaggcg
      481 aagacccatt cggctgtgcc gccgatcacg ccgatcatgc cgggagcatc agatcctgcg
      541 ttcgcgcgga aatgggcgtt gagaatggcg gagctttgat ccggtgcgtc gatgtaggga
      601 acgagcgatg tcgcgatcca cgacggtacc gcgagaatca cccgatcgtc cgtggacaga
      661 tcgatacgct gatcgcccag atcgagcccg gtggcatgag agttcccgaa atccagggac
      721 cgcacgcgtt cgccgagccg gacgcgcgcg ctcttgccgg ccagaaatcc gagggccggc
      781 tcgatgaacg ctgccgcgag attgggcgtt gcgatgcggg gcagacaatg gcggcccccg
      841 cgcgcgagcg tttcgcgcaa tatcgcggaa gcaaggtcgg cagacgcttc ttcgggcgca
      901 gagttgagcg ccgcgagcag caatggccgc ataaggcgct cccaaagcgg tccggcgcag
      961 gtgatgacgt ccgcaacgcg ccggccgcgg ttggcgatca aaagtcgggc gattttcgca
     1021 taatccaggg cccccgtgcc ggggacgcgc ctgttgccag aaaatatcca ccagggcact
     1081 gcgcccgcat tgggagcgat cgtccagcgc tcgcccgatc ttacgtcgca gaagatgaag
     1141 cgcgcttcat ccggtccatt gagacggtcc cccgcgccaa ttgcgctcag ataggagaag
     1201 gttgcgtgat tgccgctcag aacaagatga ttcccgttat caatcacctc cccaatctgc
     1261 gcgtcgaacc aggaacggca gcggcctccc ggctgcggcg cagtctcgat gatttcgacg
     1321 cgcatgccgc gcccggcgag ttccaccgcc gcagacagac cggcgagacc tgccccgatc
     1381 acgtagacgg ttccgttaat catgccggaa tgccgtagcg cagaaaaaac cagagcaacc
     1441 gggtctttgc gatgcgcacg cgcgagcggg gcggcgccca tccctgccgt tccatcgcat
     1501 cgagaatcga ggaatagacg gcgctcatca actttggcgc tcgcagatcg ccgcgcggtt
     1561 ggctggcaat aatcgcgtct gcctcgcggt agtgctgccg cgcgattcgt gcgacgccgc
     1621 ggcacggcgc gtcgatcgcc ggcgaagcaa caactttgtc cgggttgtcg attgcaatgg
     1681 ccgcctgatc gagcagttca cgcggcaggt aaagtcggcc aagacccgcg tcctcatcga
     1741 gatcccgaag tatgttggtc atctgcaacg cgcggccgag atggtgggcg aggcgaaaac
     1801 ccggttcttc gtccatgccg aaaatcttga tcgaaagccg tccgacagcg cttgccaccc
     1861 gctcacaata gagatccagg gtcgccgcgt cgggagcgca gatatcctcc gccacatcca
     1921 tcgccatgcc gtcgatgacg gcgagaaaat cctccttgcg cggtcgataa cggcgaacca
     1981 ccgcttccag aaacgcggcg cgctgagcgg cggatcctgc atagagagct tcgacatctc
     2041 ttcgccacgc atcgagcctg gcgctgcgga tttcccgcgg gtccccgcca ttatcggcaa
     2101 tatcatcgac ggcgcggcag aaagcgtaga tcgcatacat cgcttcccgt tccgccttcg
     2161 gcatcaggcg catggccgca taaaacgagc tgcccgacgc tttttgttgc gtcgcacgcg
     2221 cttcagtttc aatcgccatg cgcggtttcg tggggatgag atgagagggc caaacgggac
     2281 gccttcgcgc gacggcgcgt gacaaggaac tccgcgatcg ccaccagggc acacccggtt
     2341 gtttccagca tgttgtgatg cacccgctcc gagagcggat cctgcgtgag aagccgccgg
     2401 ttcaggtcct cggcaagccg ctggatcaag gccacctcca acgccaggcg atggtctgcg
     2461 atctggcttg caaacggcct ggattgcgca agcagcgacg aatttcgctg cgcgatttgt
     2521 gcaatgacct gttgcaaggc cggcggagac cgcgccgcgc ccagcgcggc aggttccagc
     2581 ccgcgctcct cgagcacgtc gagaggaatg tagacccgat cgagattgcg aaaatccttg
     2641 ccgcaatcct gcaggtggtt gatcacctgc aacgccgcac aaagcgcgtc cgatgccggc
     2701 cacgcggcgc gcgactcccg atgcacctca agcacaaaac gcccgactgg catggcggaa
     2761 taccggcaat attccatcaa ttcgtcccag tttgcgtaac gcagcttggt cacatcgcgg
     2821 cggaaagctt cgagaagctg caacgcgtgc tggggagaaa gattctctcc cgcgagaatg
     2881 tcgcgaagtc ttgccgcttc ggccgaaaga tcggcctcgc cgtgcagcgt gcgccacatg
     2941 ttttcgagca gatgcagttt ctctgccggc tcggcgcacg gatggtcggc tatgtcgtca
     3001 gccgcgcgcg caaatctgta gaaggcaaga atgatcgggc gatgccgctt gcggatcaga
     3061 aaggatgcga cgggaaaatt ttcgtcgcgg tgtcccttgc ccgacgagaa tgaaggttca
     3121 ctcatgacgc gtcacgccgg cgcctgggcg cggcccttcc acatgccgcc gcgcccgcgc
     3181 cagtgctgaa cggctgaatc aatggtgaac cccgcataga tggcgccgat tgccggcagt
     3241 gccgcacccc agaccggcgt cagccgatag aatttcagaa tcggctgata ggcggcggcc
     3301 atcagcagcc acgcaagcgc accctcgacc ctggcgttac cctgggcgaa cagcgcgaga
     3361 agcggcggcg cagcgtagac agtcagcatg ccgagcaccg tgccacagag cagagccggt
     3421 gagtagcgaa gctgggcata ggcggatcgg gcgaccatgc ggccgatgtc ggcgaaggcc
     3481 gaataggagc gcaagctggt tgcccgattg gtcagtccga gccatgccgg tccctgctgc
     3541 ttcaaaatcc gggcgagcgc gcagtcatcg atgatcgcac ccgcaattgg ctccagtccg
     3601 cctgccgcaa ccaacgcgtc ccgccgaacc agcatgcagc cgccagccgc cgccgctgtc
     3661 gtccgcgtcc ggtcgtttgc ccaggcgaat gggaaaatca tcgcgaaaaa gaatacgaat
     3721 gcgggaacaa gcagccgctc tgcgaagctt tcgcaatgga gcttcgccat cagcgacacg
     3781 agcacgaggc ggccagattc ggcgcgacgg acgaggcgcc ggaggttgtc gggtgcgtgg
     3841 gcaatatccg catctgtgaa aagcaaatag tcgggggcgc cggtttcgga cacgcgcctg
     3901 aaaccttgat gcatcgccca caccttgccc gtccagccag ccggccggga ttgcccgcgc
     3961 agcagctcga aactttcgct tcctccgttc atacgcgccg cgcgaacagc ttcttctgcc
     4021 gttccatctg tactctgatc gtcgatcagg acgatgcgaa agcttcccgg ataatcctgg
     4081 gcgcgcagac tagcgaccgt ctgcacaatc acatccgcct cgtcgcgggc gggaacgatc
     4141 gcaacaacag acggccaact ttgctggatt tcgagcgagc ctccgcgatc atcccgttcg
     4201 cgcatcagcc agaaaaatcc tcgtgcgagc aggaggtagg tccagacgag gagcgggacg
     4261 agtgcgacac cagtcatcgc agatagcccg cgccgcggta ccacgcgatc gcctcgttga
     4321 gagcctcgcg gtacggacgg gcgcgatagc ccagctcccg ctcagttcgt accgaggtga
     4381 aaaacatgtg atgccccgcc atccgcaacg cgtcgcgggt gaggaaaggc tcctttccgg
     4441 tcagccccgc cacggcttct gctgcgaacg cgagcggaaa gagcggcgtg cgcggcaatt
     4501 cgaaacgggg cgccttgcgg cccacgagtt ccgcgatggt
//
LOCUS       DASZMC010000359         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_445970,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000359 DASZMC010000000
VERSION     DASZMC010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445970"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VG274_07370"
     CDS             <1..251
                     /locus_tag="VG274_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HWE06511.1"
                     /translation="DHEHTYRFFIQLAYVGAMHIANFLVALAIGAVERDWSAAFFVMA
                     VATGLSAWSLVRRTKLPLAILLVIALAILGVGNGGPAA"
     gene            553..1185
                     /locus_tag="VG274_07375"
     CDS             553..1185
                     /locus_tag="VG274_07375"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWE06512.1"
                     /translation="MAEKARDERSTHAAQQDATRGMQQDATQAAREATRKNADQAERF
                     GRAAADANTQAARVGADIMGRNIETTHHVLQSGADMAAKIAKRSAQQFGRAFGFAGND
                     AEKAAQTFSTNVHAILESSATLADMAQQVAAEWTSFGRDHLERNFDRWDHLFESRTPQ
                     DLLAAQSEMMKNNLEGLLGYTRRVAEHSMRTTEELTQKFNRAAERSQPAA"
     gene            complement(1189..1947)
                     /locus_tag="VG274_07380"
     CDS             complement(1189..1947)
                     /locus_tag="VG274_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWE06513.1"
                     /translation="MCGRFAITLPPEAVRRFFQYFEQPNFPPRYNIAPTQPVPVVRLA
                     EGARQFALLRWGLIPSWVKDPKSFSLLHVGRAESVNEKPAFRNAMKRRRCLLPADGFY
                     EWSEFGERKRPFFIRPKQGNKAGGMIAFAGLWETWMGPNGEELDSVAIITTPANPLLG
                     RIAERMPAILPPESFDLWLDCAKVDALTAAAFLVPAPEALLEAYPVSTAVNRTANDSA
                     ELIAPVSPEAPPAPAPPAPKRTRKASDDRQGSLF"
BASE COUNT          406 a          627 c          625 g          333 t
ORIGIN      
        1 tcgatcatga acacacctat cggttcttca ttcagctggc ctatgtcggc gcgatgcaca
       61 tcgccaattt tctcgtggcg ctcgccatcg gcgcggtcga acgggactgg agcgctgcgt
      121 tcttcgtcat ggccgtcgcg accgggcttt cggcatggag cctcgtgcgc cggacgaagc
      181 tcccgttggc gatcctgctg gtgatcgcgc tcgcgatttt gggagtggga aacggcggcc
      241 ctgcggctta ggcgcaatga gccgggcgag cgatcgccgc cgctggcacc gccgccgcgc
      301 tggccggtca gcgccgtcgc agcttaaaaa ctgacctcgg ctcggcccga gggccccgcg
      361 atcgcaagcc gtggaagttc cacattcgaa ctcgccggcc gtttcaacgg cgaatgatcg
      421 gacaatcaag gtacagttgc gaaagatttg cgtgcacttg cgaaagattt agcccacatc
      481 ccggaacgga ttctgacagg gcgacttcca cagctatcca tctcatcacc agctcttcca
      541 agggaggtca ccatggccga gaaagcaaga gacgaacggt ccacccacgc ggcacagcag
      601 gatgctacac gaggcatgca gcaggacgcg acgcaagcgg cgcgcgaagc aacccgcaaa
      661 aacgccgatc aggccgagcg cttcggacgc gccgccgcag atgcaaacac gcaagccgcc
      721 agggtcggtg ccgatattat gggtcggaac atcgaaacca cgcatcacgt cctgcaatcg
      781 ggcgcggaca tggcggccaa gatcgcgaag cggtcggccc agcagttcgg acgcgccttc
      841 gggttcgcgg gcaatgatgc agaaaaggcc gcgcaaacct tctccaccaa cgtgcatgcg
      901 attctcgaat cgagcgcaac acttgcggat atggcgcaac aggtcgccgc ggagtggaca
      961 agcttcgggc gcgaccatct cgagcgaaac tttgaccgct gggatcatct gttcgagtcg
     1021 cgcaccccgc aggatttgct tgcagcgcaa agcgagatga tgaagaacaa tctcgagggc
     1081 ctgttgggat atacgcggcg cgttgccgag cactcgatgc gaaccaccga agagctgacc
     1141 cagaagttca atcgagccgc cgaacgatcg caacccgcgg cgtgaccttc agaacaacga
     1201 tccctgccgg tcgtcggagg ctttccgcgt gcgcttgggc gccggcggag cgggtgccgg
     1261 cggcgcctcc ggcgaaaccg gtgcgatcag ctctgccgag tcgttggcgg tgcggttgac
     1321 ggcggtcgag accggatagg cttcgagcag cgcctcgggc gccggcacca aaaacgcggc
     1381 tgcggtcagc gcatcgacct tggcgcagtc gagccaaaga tcgaagcttt ccggcggcag
     1441 gatcgccggc atgcgctcgg cgatccttcc gaggaggggg ttcgccggcg tggtgatgat
     1501 cgcaaccgaa tctagctcct cgccgttcgg tcccatccag gtctcccaca atcccgcaaa
     1561 cgcaatcatg ccgcccgcct tattgccttg cttggggcgg atgaagaacg ggcgcttgcg
     1621 ctcgccgaat tcgctccatt cgtagaagcc gtcggctgga agcaggcagc gccgccgctt
     1681 catggcgttg cggaacgcag gcttctcgtt gaccgattcg gcgcgcccca cgtgcaacag
     1741 ggaaaagctc ttcgggtcct tgacccaaga tggaatgagc ccccagcgca gcagcgcgaa
     1801 ttgccgcgcg ccctccgcga ggcggacgac aggcaccggc tgggtcggcg cgatattgta
     1861 gcgcggcggg aaattcggct gctcgaaata ctggaaaaat ctccgcacgg cctccggcgg
     1921 caaggttatc gcaaagcgac cgcacatgct taaaagatcc tcaacgggca ttaactattc
     1981 ttaaagaacc t
//
LOCUS       DASZMC010000360         3090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_446263,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000360 DASZMC010000000
VERSION     DASZMC010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446263"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..696)
                     /locus_tag="VG274_07385"
     CDS             complement(43..696)
                     /locus_tag="VG274_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWE06514.1"
                     /translation="MRLPIAGIVRDFSDQQGSLLISRDVYLRAWHDDTVNVFRLYLAP
                     GAKEADVRQRIIDMFGKTQRLFVLTTGDVRQFVQRLTDQWFGLTYVQIAVAVLVAVLG
                     VVNALTVSITDRRRELGVLQAVGALRQQIRHTVWIEALSIGVIGLALGWLLGAITLFY
                     AVGIARRDLIGIEIGYSYPIGIALTLVPVILIAAFLAALGPGEAAVKGSLVEALEYE"
     gene            complement(636..2582)
                     /locus_tag="VG274_07390"
     CDS             complement(636..2582)
                     /locus_tag="VG274_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWE06515.1"
                     /translation="MMLWRLISLPYARKHLLRWLLTTAGIVLGVGVFVGMHTANRSVL
                     AAFRETIDRIAGSTQLQVTAGEPGFDEDVLEKVQNIPEVRAAAPVIEATVSTASGNLL
                     ILGVDMLGDRSLRTYDLEDSDTSIDDPLVFLAQADSLLVTKTFAEKNGLHVNSRLPMR
                     TMQGDLTFTVRGIMKPGGLASAFGGDLAIMDIYAAQKFLGRGRKFDRVDLALADGTTL
                     DQGIAKLRAALGPGYEIAPPSSRGEQFEQTSRIYSLASDITSVFALFIGMFIIYNTFA
                     IAVTQRRTEIGILRALGATRRQIRLLFLGESAIAGLCGSILGVLFGIALARAMAGYIG
                     GILTEVYGVAQRAGAIAIDPWLMGAALAMGIATSMIAAVIPSRAAAAVDPVKALQKGR
                     NEAVGERENRLRQWAATICALASVVALLLSRNAIIFYAGFVLTVITAVLLAPALTLWL
                     ARLLRPLLQWARPVEGTLAADSLIQGPNRTSGTVAALMLSLALVISLGGLARSSFESI
                     SEWMRIALNPDLFVTTAQSVTARTFVFPASLGEGLRKVPGVAEVQLVRSVRVNVKGAP
                     VMLIALDVHSMEQRAKLPAVEGDSADMYRRTANGESVIAPRTMFGCTAPNSERYWIFP
                     LPPERCVCRSPELSAIFRTSKAAS"
     gene            complement(2579..>3090)
                     /locus_tag="VG274_07395"
     CDS             complement(2579..>3090)
                     /locus_tag="VG274_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWE06516.1"
                     /translation="QRTRVRRDKIGFIFQFFNLLPTLTSLENVGLPLHLRGWPRAKID
                     ARVRELLNLVQLGHRLDHLPDELSGGERQRVAIARALSVYPPILLADEPTGNLDTATG
                     VEILKLIHDVHDRLGSTVLIVTHDASVAESCERTLRIRDGHIVEDIRREANRRSESRG
                     PAPQETTRA"
BASE COUNT          625 a          990 c          950 g          525 t
ORIGIN      
        1 gccacggcgg cgaagcacgt ggcaaaaaaa gcgacgattc tcctactcat attccagagc
       61 ctcgaccagc gatcccttga cggcggcttc tcccgggccg agcgcggcca aaaacgcggc
      121 gatcagaata acggggacca atgtcaacgc gattccgatg ggataggaat agccgatctc
      181 gatgccgatc agatcgcgcc gcgcgatgcc cacggcgtag aacagagtga tcgcacccaa
      241 cagccatccg agcgccagac caatcactcc gatggagagc gcctctatcc aaacggtatg
      301 acgaatctgt tgccgcagcg cgcccacagc ctgcagcaca ccgagctcgc gacgccggtc
      361 cgtaatagac accgtcagcg cgttcaccac gccgagcacc gccaccagca cggcgaccgc
      421 gatctgcacg taggtgagtc cgaaccactg atcagtcagg cgctggacaa attgccgcac
      481 gtcgccagtg gtgagcacga acaggcgctg tgtttttccg aacatgtcga tgatgcgctg
      541 ccgcacatcc gcttctttcg cgccgggcgc caggtaaagc cggaaaacgt tgaccgtgtc
      601 gtcatgccag gcgcgcagat atacgtcgcg cgagatcagg aggctgcctt gctggtccga
      661 aaaatcgcgg acaattccgg cgatcggcag acgcaacgtt ccggagggag cgggaatatc
      721 cagtacctct ccgagtttgg cgccgtgcag ccgaacatag ttctcggggc gatcaccgat
      781 tctccattcg cagtgcggcg gtacatatcg gcggagtcgc cctccactgc cgggagcttc
      841 gcacgctgct ccatcgaatg cacgtccagc gcgatcagca tgacaggcgc gccctttacg
      901 ttcacgcgga cactgcgcac cagttgcact tcggccactc ccggcacttt gcgcaatccc
      961 tcgccaagcg aggcgggaaa gacgaatgtg cgcgccgtaa cgctctgcgc ggtggtgacg
     1021 aataagtcgg ggttgagcgc gatgcgcatc cactcggaaa tcgattcgaa gctggaacgc
     1081 gccagaccgc cgagcgaaat cacgagcgcg agcgagagca tcagcgcggc gacggtgccc
     1141 gaggtgcggt tcgggccctg aatcagacta tcggctgcca gcgtcccctc gacgggccgc
     1201 gcccactgca gcagcgggcg cagcagacgc gcgagccata aggtaagcgc gggcgcgagc
     1261 agcacggccg taatgaccgt tagaacaaat ccggcataga agatgattgc gttgcggctc
     1321 agcagaagag cgaccaccga cgcaagtgcg cagatggtgg ccgcccattg acgtaggcgg
     1381 ttctcccgct cgcccaccgc ttcgttgcgt cctttttgca gagccttgac cgggtctacc
     1441 gccgcggccg cacgcgaggg aatgacggcg gcgatcatgc tggtggcgat gcccatggcc
     1501 agcgccgcgc ccatgagcca cgggtcgatc gcgatggcgc ccgcgcgctg cgctacgccg
     1561 tacacctccg tgaggatgcc gccaatatag ccggccatgg cgcgtgccag ggcgatgccg
     1621 aacaacacgc caaggatcga cccgcacaaa cccgcgatcg cgctttcgcc caggaacagc
     1681 aggcgaattt ggcgccgcgt ggcgcccagc gcccgcagaa tgccgatctc cgtccgccgc
     1741 tgcgtcaccg cgatagcgaa cgtgttgtag atgataaaca ttccaatgaa cagcgcgaac
     1801 acgctggtga tgtccgacgc caaagagtag atgcgtgagg tttgttcgaa ttgttcgccg
     1861 cgcgagctcg gcggcgcgat ctcgtaacct gggcccaaag cggcgcgcag tttggcgatg
     1921 ccttggtcga gcgttgtgcc gtcggcgagc gccaggtcca cgcgatcgaa cttgcggccg
     1981 cggcccagga acttttgcgc ggcgtaaata tccatgatcg cgagatcgcc gccgaacgcg
     2041 ctcgccaggc cgccgggctt catgatgccc cgcaccgtaa acgtcagatc gccttgcata
     2101 gtgcgcatag gcaagcggct gttcacatgc aggccgttct tttcggcaaa cgttttggtg
     2161 accagcagag aatccgcctg cgccagaaac accagcggat cgtcgatcga cgtgtcgctg
     2221 tcttccagat cgtacgtgcg caggctgcgg tctcccaaca tatccacgcc cagaatcaaa
     2281 agattgccgg acgcggtgga caccgttgct tcgatcacag gtgcggcggc gcgcacctcc
     2341 ggaatgttct gcactttctc aaggacgtct tcgtcgaaac ccggctcacc agccgtgact
     2401 tgcagttgtg tcgatccagc gatgcggtcg atcgtctcgc gaaatgccgc caggacgctt
     2461 cgattcgccg tgtgcatgcc gacgaaaacc ccgacgccca acacgatgcc cgcggtggtt
     2521 agcaaccagc gcagcagatg tttgcgcgcg taaggcaggc tgataaggcg ccacaacatc
     2581 atgcgcgcgt ggtctcctgc ggcgccgggc cgcggctctc acttcggcga ttggcttctc
     2641 tgcgaatgtc ttccacaata tggccgtcgc ggatgcgcag cgtgcgctcg cagctttcgg
     2701 ccacgctcgc gtcatgcgtc acgatcagca ccgtggagcc aagcctatcg tgcacatcgt
     2761 gaatcaactt caggatctcg acacccgtgg ccgtatcgag attgccggta ggttcgtcgg
     2821 ccagcagaat gggcggataa acgctgagcg cgcgggcgat ggccacgcgc tgccgctcgc
     2881 ctccggaaag ttcgtcggga agatgatcca ggcgatggcc cagttgaacc aggttgagaa
     2941 gctcccgcac acgcgcatcg atttttgcgc gcggccagcc acgcaggtgc aggggcagcc
     3001 cgacattttc gagcgacgtc agcgtgggca gcagattgaa gaactggaat atgaatccga
     3061 tcttatcgcg gcgtacgcgc gtccgctggt
//
LOCUS       DASZMC010000361         7013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_726735,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000361 DASZMC010000000
VERSION     DASZMC010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726735"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1628)
                     /locus_tag="VG274_07400"
     CDS             complement(219..1628)
                     /locus_tag="VG274_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWE06517.1"
                     /translation="MDSHKTYFLCGIGGSGMLPLALILRAQGHRVAGSDRALDQGRTA
                     QKFDFLETRGIALFPQDGSGIESANQIAVISAAVEDSVPDVVAARRIGAPIVTRAELL
                     AQLFNAAPMAVGVAGTSGKSTTTGMIGWILESVGLEPTVMNGAVMKNFMTSSVPFASA
                     VVGKGDSFVSEVDESDGSIALYQPRVAVVTNIALDHKSLDELRVLFADFVAKSHMAIL
                     NLDNDESARLAACLPRDRMVSFSLADPAAQMLGVKLSPRPEGIAFEVRERDSDARAAV
                     ELRVPGRHNVQNALAALATARVCGVSLEDAALSLAGFSGLRRRFEIVGSAHGVTIIDD
                     FAHNPDKIAATLATAHDFPGRLLLLFQPHGFGPLRLMKDQLIDAFARGMREGDVLIMP
                     DPVYFGGTTERSTTSADIVEGVAARGHKARAIADRAACGEALVAEARPGDRIIIMGAR
                     DDTLPTFAADVLRTIEQRG"
     gene            1679..2512
                     /locus_tag="VG274_07405"
     CDS             1679..2512
                     /locus_tag="VG274_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HWE06518.1"
                     /translation="MRAVKIGVVAPGSRIDPPLAERATKLAAELYGERVSLVVHPQCF
                     LVSGHFAGDDAARANAFLEMANDASFEALWIARGGYGACRIVPNVLQGLTPAAMGKIY
                     LGYSDAGSLLAALYGRGMRRIVHGPMPADMLRAGGEVAVARALAFLVERSPAALDASV
                     TGEIPTAAFNLTILSHLLGTPFVPDLAGHVVMLEDVSEHLYRIDRALCQVTSNPGIRR
                     AAGLRLGRISDVPPNDPPFGQTPEEIIAHWCAVSGIPYLGRADIGHDIDNKIVPFGKL
                     L"
     gene            complement(2807..4627)
                     /gene="mdoH"
                     /locus_tag="VG274_07410"
     CDS             complement(2807..4627)
                     /gene="mdoH"
                     /locus_tag="VG274_07410"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HWE06519.1"
                     /translation="MTSRRSSPERWLYAGIVFSTTAVASVRLWSILRVDGFTHLKLLL
                     LTLFAILFCWISASFWLCVVGAWARSQRTKLLQPRGGEVRPTPRPRVAVMTAIFNEDA
                     RLVFARLQAMCESVCAAGAADLFDFFVLSDSTSPGCLSTEADAFRTLRANAPGRVYYR
                     HRAPNSGRKSGNIREFCENWGAAYEYMVVLDADSLMTGDTLVKLLGLMDANPRAALIQ
                     VPPLLIGRRSLFARLQQFASSVYGPLYWDGLACLQGPDSNYWGHNAILRTAPFIRHCG
                     LPKLPGRPPLGGEILSHDFVEAALLRRAGWELWMAPELGGSYEESPPTLLHYLKRDRR
                     WCEGNLQHLRLIFAQGFRGSSRLHLAVGVMSYLSAPLWLAMLAVASVETFREQHFAPA
                     AFAGRFPVLALPVSHAMECVVLVLVAALLLLGPKFLGLLLLARERGGLAAHGGGGRAL
                     CSVLFEILLSTLLAPIVMISQSAFVASVFLGRSTTWVAQRRSSRGIGIGAAVGSFSLH
                     TMLAVGAATLIYEYIPSCFWWLAPLLAGPALSIPLALVSSSPLAGRFAARIGLLLVPS
                     ETTPLPIAARFETLAGRDGHSTPPTPGYEDAAPAPLRVAA"
     gene            complement(4614..4838)
                     /locus_tag="VG274_07415"
     CDS             complement(4614..4838)
                     /locus_tag="VG274_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06520.1"
                     /translation="MTTGNVPQNPSPPGEGSAAELSASMPPCCRSAMPDQQIVIWTPS
                     RLAGAFFAALRAALAAPRLVVSRTSADDVT"
     gene            complement(5086..5739)
                     /locus_tag="VG274_07420"
     CDS             complement(5086..5739)
                     /locus_tag="VG274_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HWE06521.1"
                     /translation="MRRSITSKVMKAAPEKVGVITGRPPRRLIVGITGASGAIVGVRV
                     LQLLAETGIETHLVMSRAAEMALAYETDLQPKDVKVLAGVHHAIMALGAAISSGSFET
                     MGMLIAPCSIRTMSEIATGVTSTLLSRAADVVLKERRRLVLAIRETPLHLGHLRTMTA
                     LAEMGAVVAPMAPAFYTRPNSVDDIVNHTAGRLLDLFGISTSAVRRWEGGSPKRPAG"
     gene            5762..6583
                     /locus_tag="VG274_07425"
     CDS             5762..6583
                     /locus_tag="VG274_07425"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWE06522.1"
                     /translation="MTIQPSHVGEHLKDWRRRRRMSQLDLACEAQISTRHLSFLETGR
                     ARPSREMILNLAEQLQIPARERNVLLVAAGYAPIFPERSLADPALDAARRAIDLVLGA
                     LLPYPGFALDRHWNVVATNRAIPELYAGVAGELLEPPSNAMRLSLHPKGLAPRIANFR
                     EWRAHALLRLRQQVELTADPALVDLLSEVSGYAIPQGGIVHPIPPQASEFVVPFQIMT
                     QAGLLAFFTTTTVFGTPLDVTLSELAIESFFPADARTAETVTRLAEQRKPAHVGA"
     gene            6570..6986
                     /locus_tag="VG274_07430"
     CDS             6570..6986
                     /locus_tag="VG274_07430"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWE06523.1"
                     /translation="MSALDALNDNPLPLARLLGIVLRQAGRDCVVAEMTVRDDLCTLG
                     ERVHGGALMALADTAAATGTAVSLPDGAAGTTTIESKTNFVSGAALGTKLIATAAPIH
                     RGRQTQVWQTHVETEAGRLVAVVSQTQMILYPRNDA"
BASE COUNT         1304 a         2299 c         2252 g         1158 t
ORIGIN      
        1 tcttgcgcgg gggaggcggc ccgaagggcc ggagggggcg agcggccggc cgatcaaatt
       61 cccgtctgcg agaattgagc gaagacaatt caagattttg cgttgtcatt gccccctccg
      121 cccgcttcgc aggcaccccg catcgagtgc ggggcaggct ctcccccgcg cgcaagtgcg
      181 caggggagga aaaataggca taacgcaccc aaggcgcgtc aaccgcgctg ttcgatcgtg
      241 cgcaggacat cggcggcgaa ggttgggagc gtatcgtcgc gggcgcccat gatgatgatc
      301 cggtcgccgg gccgcgcttc agcgaccagg gcctcgccgc aggccgcgcg atcggctatc
      361 gcgcgggcct tgtgtccgcg cgcggcgacg ccctcgacga tgtcggcgct cgtggtgctg
      421 cgctccgtgg tgccgccaaa ataaaccggg tccggcatga tcagcacgtc accttcgcgc
      481 atgccccttg caaacgcatc gatgagttga tccttcatca gccgcagcgg gccgaagccg
      541 tgcggctgaa agagcagaag cagccgcccg ggaaaatcat gcgcggtggc gagagtggcg
      601 gcgatcttgt cgggattgtg ggcgaaatcg tcgatgatcg tgacgccgtg cgcggagccg
      661 acgatctcga agcgccgccg caggcccgaa aatcccgcga gcgatagcgc cgcgtcctcg
      721 agagaaacac cgcagactcg cgcagttgcc agcgcggcca gcgcattctg cacgttgtgg
      781 cgccccggca cgcgcaattc gaccgcggcg cgcgcatcgc tatcccgctc gcgcacctca
      841 aacgcaattc cttctggccg cggactcaat ttcacgccaa gcatctgcgc cgcaggatct
      901 gcaagactga acgacaccat tctgtcccgc gggaggcacg cggccagccg ggccgactcg
      961 tcattgtcga gattgagaat cgccatgtgc gacttcgcca cgaaatccgc gaacagaacg
     1021 cgcaactcgt ccagcgattt gtggtcgagc gcgatattgg tcacgaccgc cacacgcggt
     1081 tgataaagtg cgatggaacc gtcgctctca tccacttcgc tgacgaagct gtcccccttc
     1141 ccgaccacgg cgctggcgaa gggcacggat gaggtcatga agtttttcat caccgcgcca
     1201 ttcatgacgg ttggctccag gcccaccgat tccagaatcc agccgatcat tccggtcgtc
     1261 gtcgattttc cgctggtgcc ggcgacgccc accgccatcg gcgcagcgtt gaacagctgc
     1321 gcgagcaatt cggcgcgggt cacgatcggg gccccgatgc ggcgcgccgc aacgacatcg
     1381 ggcacgctgt cctcgaccgc cgccgaaatc accgcgatct gattcgcgct ctcgatcccg
     1441 ctgccgtcct gggggaacag cgctatgccg cgcgtttcca ggaagtcgaa tttctgggcg
     1501 gtgcggccct ggtcgagcgc gcggtccgac cccgccacgc ggtggccctg cgcccgaagg
     1561 atgagcgcaa ggggcaacat gccgctgccg ccaatgccgc agaggaaata ggttttgtgg
     1621 gaatccatcg ccggaagcta taaggcgcgc cgagggctga caagaatcgg cgggttcgat
     1681 gcgggcagtc aaaatcggtg tcgtggcgcc gggctcgcgc attgatcccc cgcttgcaga
     1741 acgcgcgacg aaactcgccg ccgagctcta cggcgaacga gtcagtctcg tcgttcatcc
     1801 gcagtgcttc cttgtctcag ggcatttcgc cggcgacgac gcggcgcgtg cgaacgcctt
     1861 tctcgaaatg gcgaacgatg cgagcttcga agcactgtgg atcgcgcgcg gaggctatgg
     1921 cgcctgtcgc atcgtaccaa acgtcctgca ggggctgacc ccggcggcaa tggggaagat
     1981 ctatctcggt tacagcgacg cgggctcgct gctcgccgct ttgtatggcc gcggaatgcg
     2041 ccggatcgtg catggcccca tgccggcgga catgttgcgc gccggcggtg aagtcgcggt
     2101 cgcgcgggcg ctggcgtttc tcgtcgagcg ttcgccggcc gcgctcgatg cgtcggttac
     2161 cggtgaaata ccgacggcgg cgttcaatct cacgatcctg tcgcatcttc tcggcacgcc
     2221 tttcgtaccc gatcttgccg ggcacgtcgt catgctggag gacgtgtccg agcatctgta
     2281 ccggatcgac cgcgcgctct gccaggtgac gagcaatccg ggaatccgcc gcgcggcggg
     2341 attgaggctg ggacgcatca gcgatgtgcc gccaaatgat ccgcccttcg gtcagacgcc
     2401 cgaagaaatc atcgcgcatt ggtgcgcggt ttcgggaatc ccctatctcg gccgcgccga
     2461 catcggccac gacatcgaca acaaaatcgt gccgttcgga aagcttttgt aggccgaaga
     2521 cccctcaccc gcgccgggaa cagaaatcgt tgcacgtatt tgcatccctc tcccatccgc
     2581 ctacgccaag gcttcggcgg accaagaggg actcctgacc cgccgtaccc ccggatcaca
     2641 gtccgggggg taggcgggcg gggggagagg ggaaacattc gagtcctcgc caccgctccg
     2701 aaatgggtga ggggtcgctc gggccgcgtt atcctggcgc tcaaacgcct gcgcgggcca
     2761 tgacaaggca tgactgcgtg atccaagacc aaaaaaatcc cgacgcctac gcggctacgc
     2821 gcaggggcgc cggagcagca tcctcgtaac caggggttgg cggagtcgaa tgaccgtcgc
     2881 gtcccgcgag ggtttcgaag cgcgctgcga tcggcaatgg cgtcgtctcg ctggggacca
     2941 gcagcaggcc gatgcgcgcg gcaaagcggc cggcgagagg actgcttgaa acgagcgcga
     3001 gcggaattga aagggcgggg cccgcaagca gcggcgccag ccaccagaaa cacgaaggga
     3061 tgtattcgta gatcagcgtg gccgccccca ccgccagcat cgtatgcaga gaaaaagagc
     3121 caaccgccgc gccgatgcca attccccgcg atgagcgccg ctgcgcgacc caggtcgtgc
     3181 ttctgccgag gaacacgctc gcgacgaatg ccgattgcga gatcatgacg attggcgcaa
     3241 ggagtgtgga gagcaggatc tcgaacagaa cgctgcagag cgccctgccc ccgccgccat
     3301 gtgcggcgag tccgccgcgt tcgcgcgcga gcaggagcaa gcccaggaat ttcggtccca
     3361 gcagaaggag agcagcgacg agaacaagca ccacgcattc catcgcgtgc gagacgggga
     3421 gcgcgagaac cggaaaacgg ccggcgaacg cggcaggcgc aaaatgctgc tcgcgaaatg
     3481 tctccaccga agcgaccgca agcatggcga gccacagagg tgcggagaga tagctcatca
     3541 cgccaacggc gagatggagc cggctggagc cgcggaagcc ttgcgcaaag atgagccgca
     3601 ggtgctgcag attgccctcg caccagcgcc ggtcgcgctt gagatagtgg agcaatgtcg
     3661 gcggcgactc ttcgtagctg ccgcccagtt cgggcgccat ccacaattcc cagccggccc
     3721 ggcgcagcag cgccgcttcg acgaagtcgt gactcaggat ttcgccgccc agcggcggcc
     3781 ggccgggcag cttcggcagc ccgcaatgcc gaatgaacgg cgccgtgcgc aggatcgcat
     3841 tgtgtcccca ataattgctg tccggtccct gcaaacaggc gagcccgtcc cagtagagcg
     3901 ggccgtaaac gctggaggcg aattgctgaa gccgtgcaaa cagcgagcgc cgcccgatca
     3961 gcaggggtgg aacctggatc agcgctgcgc gcgggttggc gtccatcagg ccaagcagct
     4021 tcaccagcgt gtcgcccgtc atcaggctgt cggcatcgag caccaccatg tactcgtagg
     4081 ccgcgcccca attttcgcag aactcgcgga tgttgccgct cttgcggccg ctgttcggcg
     4141 cgcggtggcg ataatagaca cggccgggtg cgttggcgcg cagggtgcgg aatgcgtccg
     4201 cttcggtgct cagacagccg ggactggtgc tgtcgctcag cacaaagaaa tcgaaaaggt
     4261 cggccgcgcc cgccgcgcag acggactcgc acatcgcctg gagccgcgca aagaccaggc
     4321 gcgcatcttc gttaaatatc gccgtcatca ccgcaacgcg cgggcgcggc gtgggccgaa
     4381 cttcaccacc gcgcggctgc aggagttttg tccgctgcga tcgtgcccag gcgccgacaa
     4441 cgcagagcca gaacgatgcc gaaatccagc agaacagaat ggcaaaaagc gtgagcagca
     4501 aaagcttgag atgcgtgaat ccgtcaaccc gcaggatgct ccacaggcgg accgatgcca
     4561 ctgccgtcgt cgaaaacacg atgcccgcat aaagccaccg ttcgggagat gacctacgtg
     4621 acgtcatctg ccgatgtgcg tgaaacgacg agccgcggcg ccgcgagcgc agcgcgcagc
     4681 gcggcaaaga atgctcccgc gagccgcgaa ggagtccaga tgacaatctg ctgatccggc
     4741 attgcggatc ggcaacaggg cggcatcgat gccgagagtt ccgcggcaga gccttcgccc
     4801 ggcggggacg ggttctgcgg cacattgccg gtggtcatgg ctgtcggaat ttgcggccac
     4861 atgagccaga atcctttttt ttcgcggttc tgccgtgcgg gatacccgcc ggttcctgag
     4921 cggacagcca gcctccctgc gcagccgcaa cgctgctgct caacttttgt tccttaatga
     4981 ggcattgacc gggatgcgcg tagactcaac gccgcgcgcc aagcgcggcc ctaagggccc
     5041 gtcatggcag aattttgacg gtcgcagccg atttgccaat gccgctcagc cggcgggtcg
     5101 cttcggggac ccgccttccc accgtcgcac cgcgctggtg gagattccga acaggtcgag
     5161 gaggcggccc gctgtgtgat tgacgatgtc gtcgacggaa ttcgggcggg tgtagaaggc
     5221 cggcgccatc ggcgcgacca ccgcccccat ctcggcgagc gccgtcatgg tccgcaggtg
     5281 acccagatgc aacggcgtct cgcgaattgc cagcacgagc cggcggcgtt ctttcaacac
     5341 gacatcggca gcacgtgaca aaagcgtgct ggtgaccccg gttgcgatct cgctcatggt
     5401 gcggatcgag catggcgcga tcagcatgcc catcgtctcg aacgagcccg acgaaatcgc
     5461 ggccccgagc gccatgatgg cgtgatgaac acccgccagg actttcacgt ccttcggctg
     5521 aaggtcggtt tcataggcga gcgccatttc agctgccctg ctcatcacca gatgcgtctc
     5581 gatgcccgtc tcggcgagca gctggagaac cctcacgccg acgatcgctc ccgacgcacc
     5641 ggtgattccc acaatcagcc gtctcggtgg acggccggtt atcacgccca ccttttccgg
     5701 cgcggccttc attaccttcg acgtaatgga tcggcgcacg ttcccctcct aagctcggct
     5761 tatgaccata cagcccagcc atgtcggcga acatctcaag gattggcggc ggcgccggcg
     5821 gatgagccag ctcgatctgg cctgcgaggc acagatttcg acacggcatt tgagttttct
     5881 cgagacgggc cgggcgcggc cgagccggga gatgatcctc aatcttgccg aacagctgca
     5941 gattccggcg cgcgagcgca acgtgctgct ggtggcggcc ggctatgcgc ccatttttcc
     6001 cgagcggtcc ctcgccgatc ctgctctcga cgcggcgcgc cgcgcgatcg atctcgtcct
     6061 cggcgctctt ctgccctatc ccggcttcgc gctcgaccgc cactggaatg tggtcgctac
     6121 caaccgcgcc attcccgagc tgtacgcggg cgtcgcgggg gagctcctgg aaccgccgtc
     6181 caacgcgatg cggctcagcc tgcatccgaa aggtctggcg ccgcgcatcg ccaatttccg
     6241 cgaatggcgg gcacatgcgt tgctgcggct gcgtcaacag gtcgaactca ctgccgatcc
     6301 ggcgctggtg gatttgctga gtgaagtatc cggttatgcg attccacagg gcggaatcgt
     6361 ccacccgatt ccgccgcagg cctccgagtt cgtcgtgccg ttccagatca tgacgcaagc
     6421 cggacttctc gccttcttca ccaccacgac ggtgttcggc accccgctcg acgtgacgtt
     6481 gtcggagctc gcgatcgaat ccttctttcc cgccgacgcg cgcacggcgg aaaccgtgac
     6541 gcgcttggcc gaacaaagga aacccgccca tgtcggcgct tgacgcactg aacgacaacc
     6601 ccctgccgct tgccaggctt ctcggcatcg tcttgcgcca ggccggccgc gattgcgtcg
     6661 tggcggagat gacggtgcgc gacgatctct gcacgctggg cgagcgcgtt catggcggcg
     6721 cgctgatggc cctcgccgac actgccgctg cgaccggaac ggccgtcagc ctgcccgacg
     6781 gcgccgctgg cacgaccacg atcgagagca agaccaactt cgtgagcggt gcggcgctcg
     6841 gcacaaagct cattgccacg gccgcgccaa ttcatcgcgg gcgtcagact caggtctggc
     6901 agactcatgt cgaaaccgaa gccggccggc tcgtcgccgt ggtaagccag acgcaaatga
     6961 tcctctatcc gcggaatgat gcatgaccgg tcgatacaag ttaaattggg cgc
//
LOCUS       DASZMC010000362         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3176299,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000362 DASZMC010000000
VERSION     DASZMC010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176299"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..884)
                     /locus_tag="VG274_07435"
     CDS             complement(294..884)
                     /locus_tag="VG274_07435"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWE06524.1"
                     /translation="MFVTGEWMGSIIGSAAEYNRAAKAGDWHSLCDVTLAFQHPDLVK
                     LLELWRSEAGVSGIAPRRTMSPRLLKSHLRDIALYERVGEGDGRRYRVRLMGTSFAQI
                     LGDMTGKYLDDALPAAMLPRWYAALDTTLGARAPLRFLGREDTNQMSFLTGEFLSAPL
                     VADDGQVNLVLGGARYNGKRPWEDVDADARKSLGLE"
     gene            1051..1815
                     /locus_tag="VG274_07440"
     CDS             1051..1815
                     /locus_tag="VG274_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06525.1"
                     /translation="MAFADQRSDFAMLRPMGAAIETVLPAIGVSGLTAAFAASFGLSH
                     SNPNSREGVPLRPIQHRIVGASLHGCLSGQLCVALRTVFSERAVERRVRRESPLRKSV
                     VVVLNAQSLAPQCAAKPRVVRRRFRVVGARPFLVRALHIDIVVELARIFAERHDDLRA
                     DRADLHGLADVAAAAVTVFIVVARVGARHRTAQGVGQQFELGFHSFFKAPCGGFEAIR
                     FSPGGAILRSRAAFTSASLNDTHCAEPSWVARFCGP"
     gene            complement(1855..>2573)
                     /locus_tag="VG274_07445"
     CDS             complement(1855..>2573)
                     /locus_tag="VG274_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06526.1"
                     /translation="LLSQWKSAGHPVDVLSVHFYPQGNEYGNDNSTATQLLRNRSTRQ
                     LWDPNYTSESWINDKVNLIPRLKGWISSYYYEGTPVAITEYNWGDEAQINGATTQADI
                     YGIFGREGLDMATRWTVPGNSTPTYKAMQMYRNYDGADSGFGETSIQTSAPSPDDLSA
                     FAALRKDKTMTVMVVAKDLSGSTPLSLQLAHFKKAGTAQVWRLTAANTIQRVSDIAWS
                     KSKLTDTVPSPSVTLYVLPN"
BASE COUNT          428 a          798 c          816 g          531 t
ORIGIN      
        1 agcatcgtca tgaaaatgaa ggcgagcgca tagcgatgca ctacgggttg cggcggcgta
       61 tcggtcacgg atggtccctc gatcagcgga cgcgaccgta atcgcagcgg ggaccggcgt
      121 cttgtagaaa aaagccgcca cttccactcc gtcatggccg ggcttgaccc ggccacccat
      181 ccggcgtgcg tccgcgcgcc gaaatgactc tcttgcgctc gcagacgcga gcgcgatggg
      241 tggccgcctc aagggcggcc atgacgaatg agagagtgtg gcgtgataag gtcttactcc
      301 aaccccaagc tcttgcgcgc atcggcgtcc acatcttccc aggggcgttt gccgttgtag
      361 cgcgccccgc cgagaaccag attgacctgg ccgtcatcgg cgacgagcgg tgctgacagg
      421 aactcgccgg tgagaaagct catctggttg gtgtcctcgc ggccgagaaa gcgaagcggt
      481 gcgcgcgcgc ccagcgtggt atcgagcgcc gcataccaac gcggcagcat ggccgcgggc
      541 aatgcgtcgt cgagatattt tccggtcatg tcgccgagga tctgcgcgaa ggatgttccc
      601 atcagccgca cgcgatagcg ccggccgtcg ccttccccca ctcgttcgta caatgcgatg
      661 tcgcgcagat gggatttcag caggcgtggc gacatcgtcc ggcgcggcgc gatgcccgaa
      721 acgccggctt cgctccgcca gagttcgaga agtttcacga ggtcggggtg ctggaaggcg
      781 agcgtgacat cgcacagcga atgccaatcg cctgctttgg ccgcacgatt gtattcggcg
      841 gcgctgccga tgatcgagcc catccactcc cccgtgacga acactgtgtg ccgtcccgca
      901 taaccttatc cacgattcgc ataaggagcc aagaaaatct aacaacagca atgggttgca
      961 aatacacgat ttgcttgtga cgcgtgcctg ttcaaccttt ttccagtgca gccaggcgcg
     1021 ccggaaccag ttggcgcgga ggacgagatt gtggctttcg cggatcagcg cagcgacttc
     1081 gccatgttgc gcccgatggg tgcggccata gagaccgtgc ttccggcgat tggcgtttcc
     1141 gggctgaccg ccgcgtttgc ggcgagtttc gggctcagcc attcgaaccc gaattctcga
     1201 gagggggtac ccctccggcc tatccaacac cggatcgtcg gcgccagtct ccacggctgc
     1261 ctcagcggcc agctctgcgt cgcgctccgc acagtatttt ctgagcgcgc ggtcgagcgg
     1321 cgggtgcgac gagagagccc cctccggaag agcgttgtcg tcgtcctcaa tgcccaatct
     1381 cttgcgccgc aatgcgcagc caagccgcgt gttgtcaggc ggcgctttcg cgtcgtcggt
     1441 gcgcggcctt tcctcgtccg agcgcttcac attgatattg tggttgaact cgcccgcata
     1501 tttgccgagc gccacgacga tctgcgcgct gaccgcgccg atcttcacgg cctcgccgat
     1561 gtggctgctg cggcggtgac cgtattcatc gtcgtcgcgc gtgtcggcgc gcgtcaccga
     1621 accgcgcagg gcgtcggtca gcaattcgag ttgggtttcc attctttctt caaggcgcct
     1681 tgcggcggat tcgaagcgat ccgcttttcg cccggcggcg ctatcttgcg aagtcgcgcg
     1741 gctttcactt cggcctcgct caacgatacg cattgtgcag aacccagctg ggtggcccgc
     1801 ttttgcgggc catgacacgt gcgtggatat tcggacggcg gttgagcgtg gcccttaatt
     1861 cggcagcaca taaagcgtga cgctcggtga cggcacggtg tcggtgagtt tgcttttcga
     1921 ccacgcgatg tcggagaccc gctgaatggt gttggccgcg gtcaggcgcc acacctgcgc
     1981 cgttccggct ttcttgaaat gcgcgagctg tagagataac ggcgtgctgc cgctcagatc
     2041 tttcgccacg accattaccg tcatggtctt gtccttgcgc agcgcggcga aggcggagag
     2101 atcgtcgggc gagggcgcgc ttgtctggat gctggtttcg ccaaagccgg aatccgcgcc
     2161 gtcatagttg cgatacatct gcatcgcctt gtaggtcggc gtcgaattgc cgggcaccgt
     2221 ccagcgcgtc gccatgtcga ggccttcccg cccgaagatg ccgtagatgt cggcctgcgt
     2281 cgtcgcgccg ttgatctgcg cctcgtcgcc ccaattgtat tcggtgatcg cgacgggcgt
     2341 gccttcgtag taatagctgg agatccaacc cttcaggcgc gggatcaggt tgaccttgtc
     2401 gttgatccag ctctcggatg tgtagttcgg atcccagagc tggcgcgtcg agcggttgcg
     2461 cagaagctgt gtcgccgtcg agttgtcgtt gccgtactcg ttgccctgcg gatagaagtg
     2521 cacgctcagc acatcgaccg gatggcccgc cgatttccat tggctgagca gcc
//
LOCUS       DASZMC010000363         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_448918,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000363 DASZMC010000000
VERSION     DASZMC010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448918"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VG274_07450"
     CDS             complement(<1..907)
                     /locus_tag="VG274_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWE06527.1"
                     /translation="MPLPDGRSELPPVIRRADIVAFTLVALLVICLVAVLYVAKAFFL
                     PVVMAFVIGTMLSPAAGFLERHRIPRAVSAVLIVSALSAAVAFVVGLISSPLADWSTH
                     LPELASRLRDKLHVFDRPLALWQQLQSTLGGSDMLSAAPFQMPKFDWLQPTIEFLTPT
                     FTEFLLFFATLILFIASWRDLRRGLIMTCGGRASRLRTLRILNEIEEHLGGYLLTVTL
                     INLGVGTVTGLICAVTGMPNPAGLGALAAALNFFPIIGPIATFVALTVVGVVAFSTLG
                     AGLIAPLAFAGLVFLEGHFITPAIIG"
     gene            1139..1990
                     /locus_tag="VG274_07455"
     CDS             1139..1990
                     /locus_tag="VG274_07455"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWE06528.1"
                     /translation="MVAGIDMMRIAWGQIMAQAFDEDRSQSPRATRALIFAGALLLAP
                     AILVSEASAQVLGYASAPQSGFPSDNVMLAPALTDEGDGSASVIPERLRRAVVAYDTS
                     EAPGTVIIDTGNTALYYVLGQGRAVRYGVGVGREGFTWSGVQTISRKAEWPDWYPPVE
                     MIARQPYLPRFVAGGPGNPLGARAMYLGASQYRIHGTNDPSTIGKFVSSGCIRLTNED
                     VTDLFSRVNVGTKVVVLPKNSPHLEARRTATKSTTTVRPIAAQPRPAVTTLPSGGRAM
                     NVSTSSL"
     gene            2087..>2293
                     /locus_tag="VG274_07460"
     CDS             2087..>2293
                     /locus_tag="VG274_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06529.1"
                     /translation="MASRSVRQAAWGVAAVMCVAALAPVAAHGEDFLSALFGAFAGLR
                     PRAPAIPLPFATEGEPFAPPNEIHP"
BASE COUNT          460 a          706 c          750 g          377 t
ORIGIN      
        1 gaccgatgat ggccggcgtg atgaaatggc cttcgaggaa gacaagtccg gcgaaagcca
       61 gtggggcgat aaggcccgcg ccaagcgtgg aaaacgctac gaccccgacc acggtcaagg
      121 ccacgaacgt cgcaatcgga ccgataattg gaaagaaatt cagcgcggcg gcaagcgccc
      181 cgaggcctgc cggattgggc atgcccgtga ccgcacagat gaggccggta acggtgccga
      241 cgccgagatt gatgagcgtc acggtgagca gatagccgcc gaggtgttcc tcgatctcgt
      301 tgagaatccg caaagtgcgc aaccgcgagg cgcgaccgcc gcatgtcatg atcagcccac
      361 ggcgcaggtc tcgccaactc gcgatgaaca ggatcagcgt tgcaaagaac agcaggaact
      421 cggtgaaagt cggcgtcagg aattcgatcg tgggctggag ccagtcgaat ttcggcatct
      481 ggaacggggc ggccgagagc atgtcggatc cgccgagcgt gctctggagc tgctgccaga
      541 gcgccagcgg gcgatcgaac acgtgtaatt tatctctcag ccgcgatgcc agctccggca
      601 gatgcgtgct ccagtccgcc agcggcgaag agatcaggcc gaccacgaag gcgacggcag
      661 cactgagcgc gctcacgatc agcaccgccg ataccgcgcg gggaattcgg tgccgctcga
      721 gaaatccggc ggccggcgac agcatggtac cgatcacgaa tgccatcacg accggaagga
      781 agaacgcttt cgcgacgtag aggacggcga caagacagat caccagcagc gcgaccagcg
      841 tgaacgcaac gatatccgcg cggcgaataa ccggcggcag ttcgctcctg ccgtcgggca
      901 gcggcatgtc gactgcttct ttgggaagcg cacgttgatc gggaaggaca tgcaactggc
      961 tctcccccga tacagctatt gtgggcgctg agccgcgaag tcgcatgacg gaccgggctc
     1021 agttcatcct aaacccgcgc atgcggccaa agttccatcg cggaatcgtg agaagtggcc
     1081 tgcttgacag ctccagcgtc tcttccgcgt cgggtggaac cgtagagcct cggttgcgtt
     1141 ggttgccggc atcgacatga tgcgcatcgc atgggggcag atcatggcac aggcattcga
     1201 tgaagatcgc agccaatcgc cgcgcgccac ccgcgcgctg atcttcgccg gcgctttgtt
     1261 attggcgcca gccattctcg taagcgaggc gagtgcgcag gttctcggct acgcatccgc
     1321 accgcaaagt ggatttccgt cggacaatgt catgctcgcg cctgcgttga cggatgaagg
     1381 cgatggaagc gcaagcgtga ttcctgaacg actgcggcgc gcagtcgttg cgtacgacac
     1441 cagcgaagcg cccggcaccg tcatcatcga taccggcaac accgcgctct attatgtgct
     1501 gggccagggc cgcgccgttc gctacggcgt cggcgtcggc cgtgaaggct ttacctggtc
     1561 gggcgtgcag accatcagcc gcaaggcgga atggccggat tggtacccgc cggtggagat
     1621 gatcgcgcgc cagccatatc tgccgcggtt cgtggcgggc ggtccgggca atccgctcgg
     1681 cgcacgggcg atgtatctcg gtgcgagcca ataccgcatc cacggcacca acgatccctc
     1741 gacgatcggg aagttcgtct cctcgggctg catccgtttg accaatgagg acgtcaccga
     1801 cctgttcagc cgcgtcaatg tcggcaccaa ggtcgtggtg ctgccaaaga attcgccgca
     1861 cctcgaagcc aggcggacgg caaccaagag cacgacgacc gttcggccga tagcagcgca
     1921 accgcgtccc gccgtgacga cgctgccttc gggcggccgg gcgatgaacg tttcgacctc
     1981 atcgctgtag cgttttcgcg ccggttcgcg tgaagaaaac gcgtcgaaaa tagagcgcct
     2041 tcgagccgga ccgggcgcga aaacctcttt ttggaaggag gtcgagatgg cgagcagatc
     2101 ggtgcgacag gcggcgtggg gagtggcggc ggtgatgtgc gtcgctgccc tggctccggt
     2161 tgccgcgcac ggcgaagact ttctctccgc attgttcggc gccttcgcag gtctccggcc
     2221 acgcgcgcct gcgatcccgc tgccgttcgc aaccgagggc gaaccgttcg ctccgccaaa
     2281 cgagatacat ccg
//
LOCUS       DASZMC010000364         7295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_449317,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000364 DASZMC010000000
VERSION     DASZMC010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449317"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VG274_07465"
     CDS             <1..1319
                     /locus_tag="VG274_07465"
                     /inference="COORDINATES: protein motif:HMM:NF044325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HWE06530.1"
                     /translation="ATREHSIEDTAYKTNLEAAEEIARQLRLRDLAGLIVIDFIDMED
                     PRNDRNVEKRLRDNIKNDRARVQIGKISQFGLLEMSRQRLRAGVVAGSTVPCPHCGGQ
                     GIVRSVESTTLRLLRGLEEEAQRQRAAILSVRVPTEVAIYALNQKRREIAHIESGYGL
                     AIQFEAKEGMLTGTFEVERIGQHVPDERVKVQTVSEGAASAAATIAADAIEPGEPEQR
                     EEEMVRAPEAQPGSPGSSKRKRRRRRGRDRDRASPPPGADANRAITAEAITQQDSPEP
                     PASASPGNPPGKELIANGDGTVRKRRRRRRGRRGSRDNSLATLPNAQNTPVTADGPDE
                     PGVQVARPGEYEGVDQSAIPVIATPTVAPNAPSEPVWSLSSDVKHAMDVSAGRDEPLE
                     TKPAASSPTETAHSRVEAPKPSHPARDEPSGPPRKGWWQRPFRLRE"
     gene            complement(1327..2667)
                     /locus_tag="VG274_07470"
     CDS             complement(1327..2667)
                     /locus_tag="VG274_07470"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWE06531.1"
                     /translation="MFCSRIFAAAAVFFSIAACSMVGDSGSKLPGGGLLVGDEPDAVE
                     AGAQVLAHGGNAVDAATATYFALAVTYPVAAGLGGGGLCIVGDSKRSRMEAFDFLARD
                     AARAGPYAVPGNVAGFALMQANYGKLPWQRVVSPAEGAAAAGFVISQALAARLAGSQN
                     VIRLDAGLAAEFLDETGHVRPAGTLVTAPALAQSLSSIRTLGSVSFYRGPIANAIVAY
                     SEEQGGTISIEDLASYASQRSAPGLARVGNQTVYLPASRTGAGRFAATLLSHLVDANG
                     QARTTDNPNAEVASATKGSLDAYGIASLPRDLGATGFAAEDPEGLAVACAVTMNGPFG
                     SGHTASGTGITLANASSQGEAGLAAAFLTPVVAVSDGAVSLVGAGAGGPNGTAGIALA
                     LLRLARGEDVIRPGVLHSTGIAPYDTVNVIGCQNGVCAAVPDPGAHGLGASGGP"
     gene            2775..4175
                     /locus_tag="VG274_07475"
     CDS             2775..4175
                     /locus_tag="VG274_07475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWE06532.1"
                     /translation="MAICNPAKNRASTSPAFVRRCATGLLAAVCALSITVEPAAAQAL
                     QLIRDTEVERMIKSYEDPILVVAGLDPAAVKMYIVQDPSINAFAAEGQNIFVNTGLLQ
                     QLHTPNEVIGVFAHETGHIAGGHLIRDTNAISKAMIPMLIGMAAGVAAMLAGAGEGGM
                     AIMGLGMNAAQAQFMQFSRAQEGTADQMGQKFLRETHQSGQGMLDVFERLADEEAMSA
                     YKIDPMAMDHPASRERIDLLQRLVEISPYKDVKDSAQALHAFRMIQAKLAGFLDPVDL
                     VLTRYPPTDLSEEGHYARAVAYFRQPDLQKALAECDALVRIEPRNPYFWELLGQIYVD
                     MSRPEKGVPPYQQSVDLLPDAPLLRVSLAAAQLATEKSAFAKPALDNLKIALRQENDN
                     TFAWFEAAQAYSQLGNEPMANLSTAERYYNAGAMPAAAHFAILAQKKLPQGSPDWQRA
                     SDIMAVAGPETEKHKH"
     gene            4408..5124
                     /locus_tag="VG274_07480"
     CDS             4408..5124
                     /locus_tag="VG274_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWE06533.1"
                     /translation="MSGFAGAVIALTLVFCARALGLAPFAAVDASAMHSYLLAHPDVL
                     VAMTNKLQAQQDDDQDNARQAAVDKLGLKAFFDPHVAFVTGPAGATTTVVEFFDYNCP
                     YCRASVPAMKKFYETHKNARFAFIEFPIKGPLSTVAARAAMAARLQPDKYVAFHFALM
                     NEEGAVDEDNVYAVAKKTGLDVARLKSDMTSPSVDRAIDAAHRLAKAADIDGTPAFII
                     NGKIREGAMNDDDFAKMTKT"
     gene            complement(5128..6126)
                     /gene="thiS"
                     /locus_tag="VG274_07485"
     CDS             complement(5128..6126)
                     /gene="thiS"
                     /locus_tag="VG274_07485"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWE06534.1"
                     /translation="MALALNINGEERRFDRLLTVRELLIHLGLDPAKIAVERNLEIVP
                     RSSYADIRLGDGDRIEIVHFIGGGNAAIAGLPEGKPLAIAGRTYASRLIIGTGKYKTY
                     AENAAALEASGAEIVTVAVRRVNLSDPGAPRLVDYIDPKKVTYLPNTAGCFTGEEAVR
                     TLRLAREAGGWTLVKLEVLGDRKTLYPDMLETLRATELLIREGFQVMVYCSDDPLMAR
                     RLEHMGAVAIMPLAAPIGSGLGVQNAVNIRMIVEEAKVPVIVDAGVGTASDATVAMEL
                     GCDAVLMNTAIAEARNPVLMASAMRHAVEAGRLAYLAGRMAKKRYADPSSPLAGLI"
     gene            6207..6671
                     /gene="aroQ"
                     /locus_tag="VG274_07490"
     CDS             6207..6671
                     /gene="aroQ"
                     /locus_tag="VG274_07490"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWE06535.1"
                     /translation="MAQSRKRSLPIYVLNGPNLNLLGSREPAIYGKATLQQIGEATAA
                     RARSLDWEIVFRQSNREGELIDWVQEARNGARGIILNAGGYSHTSIALLDACRAVEIP
                     LVEVHLSNPYRRETFRRHSYISEAADGVICGFGAAGYLLAVDALAELLKARS"
     gene            6668..7180
                     /gene="accB"
                     /locus_tag="VG274_07495"
     CDS             6668..7180
                     /gene="accB"
                     /locus_tag="VG274_07495"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HWE06536.1"
                     /translation="MNMKDMKQLGARLATGGKAKPPFGVDAGAIRELAELLDETGLAE
                     IEIEQGGCRIRVARQAAAAPTGSHAPLPAMSEPPGAPGQAPLEGPAAKRDGAISSPMV
                     GTVYVAPEPGKPPFVKIGDTVREGDTLLIVEAMKTMNPIIAPRSGIVKEICVEDAQPI
                     EFGQTLLVLS"
BASE COUNT         1468 a         2335 c         2232 g         1260 t
ORIGIN      
        1 gagcaacccg cgagcattcg atcgaggaca cggcgtacaa gacaaatctc gaggcggcgg
       61 aggagatcgc gcggcagctg cgtctgcgcg atcttgccgg tctcattgtc atcgatttca
      121 tcgacatgga ggacccgcgc aacgatcgca atgtcgaaaa gcgcctgcgc gacaacataa
      181 agaatgatcg cgctcgcgta caaatcggca agatcagcca gttcggcctt ctcgaaatgt
      241 cgcgacagcg gctgcgcgcc ggcgtcgtcg ccgggtcgac ggtgccctgt ccgcattgcg
      301 gtggacaagg catcgtgcgg tccgtcgagt ccacgacgct gcgcctgttg cgtggactgg
      361 aagaagaagc ccagcggcag cgcgccgcaa tcctcagcgt ccgcgtgccg actgaggtgg
      421 ccatctacgc cctcaaccag aaacgccgcg aaatcgcgca cattgaatcc ggatatggtc
      481 ttgcgatcca gttcgaagcc aaagaaggaa tgctcaccgg aacgtttgag gtcgaacgca
      541 tcggtcagca cgtgccggac gagcgcgtca aggtccagac agtttccgag ggcgcagcgt
      601 cggctgccgc tacgattgct gccgacgcaa tcgagccggg cgagccggag cagagggagg
      661 aggagatggt gcgggctccc gaggcccaac ccggatctcc cgggagcagc aagcgcaagc
      721 gaagacggcg gcgcggccgc gacagggacc gcgcaagccc accgccgggc gcagacgcga
      781 atcgcgcgat caccgccgag gcaataaccc aacaggattc gccggaacca cctgcatcgg
      841 cttccccggg caatcctccg ggcaaagaat tgatcgccaa tggcgacggg acggtgcgca
      901 agcgccgacg gcgccggcgc ggccgccgtg gatcgcgaga caacagcctc gccaccctgc
      961 caaatgcgca gaacacgcca gtgaccgccg acgggccaga cgagcccggc gttcaagtgg
     1021 cgcgcccggg tgaatatgaa ggcgtcgatc aatccgcgat tcccgtgatc gcaaccccca
     1081 cagttgcccc gaacgctccg tcggaaccgg tgtggtcgct atcgtccgac gtgaagcatg
     1141 ccatggacgt gagcgcgggg cgcgatgagc cactggaaac caaacctgcc gcgtcatcgc
     1201 caaccgagac ggcccactct cgtgtggaag cccctaagcc ctcgcatccc gctcgtgacg
     1261 agccaagtgg gccgccgcgt aagggctggt ggcaacgccc attccgcttg cgcgagtagc
     1321 cgtggcttaa ggcccccctg acgctccaag cccatgggcg ccaggatcgg gcacggcggc
     1381 acagacaccg ttttggcacc cgatcacgtt gaccgtgtcg tagggtgcga tcccggtgga
     1441 atgcaggacg ccgggcctga tcacatcttc gccgcgtgcg agcctcagca aggcaagcgc
     1501 gattccggcg gtgccgttag gtcctcccgc gccggcgccg accagcgaaa ccgcgccatc
     1561 ggatacggcg acgaccggcg tgagaaatgc cgccgccaac ccggcctcgc cctggctcga
     1621 ggcgttggca agcgtaatgc cggtgccgct cgcggtgtgc ccggatccaa acggcccgtt
     1681 catcgtgacg gcgcaggcca ccgcaagacc ttcgggatcc tcggcggcaa atccggtcgc
     1741 gccgaggtct cgcggtaacg acgcaattcc gtaagcatcg agcgatccct ttgtggccga
     1801 cgcgacctcg gcgtttggat tgtcggttgt tcttgcttga ccgtttgcat ccacaaggtg
     1861 cgacagaagc gtcgcggcaa atcttcccgc gcccgttcgc gaagcgggaa gatagacagt
     1921 ctggtttcca acccttgcca gccccggagc actgcgttgc gaggcatagg atgcaaggtc
     1981 ttcaatcgag attgttccac cctgttcttc tgaataggcg acaatcgcat tggcaatcgg
     2041 gccgcgatag aagctgactg atccgagcgt gcgaatgctg gaaagacttt gcgcaagcgc
     2101 gggcgccgtc acgagcgtgc cggccggcct gacatgcccg gtttcgtcga ggaattccgc
     2161 tgcgagccct gcatcgagtc gaatgacgtt ctggctgccc gcgagccgcg ctgcgagcgc
     2221 ctgcgaaata acgaaacctg ccgcagcggc gccttcagcc ggagatacga cgcgctgcca
     2281 gggaagtttg ccataattcg cctgcatcag cgcaaagccg gccacgtttc cgggcactgc
     2341 gtagggaccc gcacgagcgg cgtcgcgcgc gaggaaatcg aatgcttcca ttctggatct
     2401 cttggaatcg ccgactatgc acaggccgcc cccgccgagt cctgccgcga ctggatatgt
     2461 gacagcaagg gcgaagtaag tcgctgttgc ggcgtcgacg gcatttccgc catgagcgag
     2521 cacctgggcg cccgcttcca cggcatcggg ctcgtcgccg acgagaagac ccccgcccgg
     2581 caacttgctg ccggaatcgc ccaccatcga gcaagccgcg atggagaaga agacggccgc
     2641 ggcggcaaaa atgcgagagc aaaacattgc aagacccggt atcgccaatc tgcacccgcg
     2701 acgcgttgac gcccgagcgg cgtgcactta ccgtcccgca cggccgagcg gaaagcggca
     2761 ggagaattca aggcttggca atttgcaacc ccgcgaagaa tcgggcgtcg accagtccgg
     2821 cttttgtgcg ccggtgcgcg acagggcttc tggccgcagt ttgtgcgctg tccatcacgg
     2881 tcgagcccgc agctgcccag gcactgcagc tcatccgcga cacggaagtc gagcggatga
     2941 tcaagtccta cgaagacccc attcttgtcg tagcaggtct cgatccggct gccgtcaaga
     3001 tgtacatcgt ccaggatcca tcgatcaacg cgttcgcggc cgagggtcag aatatcttcg
     3061 tgaacaccgg cctgctgcag caacttcaca cgcccaatga agtgatcggc gtattcgcgc
     3121 acgagaccgg gcatattgcc ggcgggcacc tgatccgcga caccaacgcc atctccaagg
     3181 cgatgatccc catgctgatc ggcatggcgg cgggcgtcgc cgcaatgctc gccggcgccg
     3241 gcgaaggcgg catggcgatt atgggtctcg gaatgaacgc ggcgcaggcg cagttcatgc
     3301 agttcagccg cgcgcaggag ggaacggccg atcagatggg ccagaagttc ctgcgtgaaa
     3361 ctcatcaatc cggtcaagga atgctcgacg tattcgaaag actggccgac gaagaggcga
     3421 tgtccgccta caagatcgac ccgatggcga tggatcatcc ggcttcgcgc gagcgcatcg
     3481 atctgctgca acggctcgtc gaaatttctc cgtacaaaga cgtcaaggac agcgcccagg
     3541 ctcttcacgc gtttcgcatg atccaggcca agcttgctgg ctttctcgac cctgtcgatc
     3601 tcgtgctgac gcgctatccc ccgactgacc tgagcgaaga aggacactac gcccgcgccg
     3661 tcgcctattt tcgccagccc gatttgcaga aggcgctcgc cgaatgtgac gcgctggtga
     3721 gaatcgaacc ccgcaatcct tatttctggg agcttctggg acagatctat gtggacatga
     3781 gccgccccga gaaaggcgtg ccgccatacc agcaatcggt tgatctcctg cccgacgcgc
     3841 cgctccttcg cgtcagcctg gcggcggcgc aactcgccac ggaaaaatct gcgttcgcca
     3901 agcccgcact cgacaatctc aagattgcgc tacggcagga gaacgacaat acgtttgcct
     3961 ggttcgaagc ggcacaggcc tacagccagc tcggaaacga gccgatggcc aatctgtcga
     4021 ctgccgaacg ctactacaat gcgggcgcga tgccggccgc cgcccatttt gcgattctgg
     4081 cgcaaaagaa gctgccgcag ggctcgccgg actggcaacg cgcgagcgac atcatggccg
     4141 tcgccggccc cgaaacagaa aagcacaagc actagcgctg gcaatcggat ttgagtgaca
     4201 tgcaagaaga ttcagcgcag tggacgcagt ggatgcgcgg agggcgcagc ggagaatgca
     4261 tggcgcgctc agcgcgcaac gtgttcgcgc gtccgctgcg catccactgc gcccactgcg
     4321 ccaagatttc tattgattga cgactcgccc cagtcgagcg gctagtatca ggtccggagc
     4381 atgaaagact tttggaaaat ggcaacgatg agcggcttcg cgggagcggt gattgcattg
     4441 acgctcgtct tttgcgcgcg cgctctcggc cttgctccct ttgccgccgt cgatgcaagc
     4501 gccatgcatt cctatttgct tgcccatccc gacgtgctgg tcgccatgac gaacaagctt
     4561 caggcgcagc aggacgacga tcaggacaat gcgcggcagg cggccgtcga caaacttggc
     4621 ctgaaagcct tcttcgatcc gcatgtcgcc ttcgtaacgg gccccgccgg cgcaacgacg
     4681 accgtggtgg agttcttcga ctacaattgt ccatattgcc gcgcctcggt tccggcgatg
     4741 aaaaaattct atgagacgca caaaaacgcc cgcttcgcgt tcatcgaatt tccgatcaag
     4801 gggccgctat cgacggttgc cgcacgcgca gcgatggcgg cgcgcctgca gccggacaag
     4861 tacgttgcgt tccatttcgc cctgatgaac gaggaaggcg cggtcgacga agacaacgtc
     4921 tacgccgtag ccaaaaaaac gggcctcgat gtggcccggc tcaaatccga catgacatct
     4981 ccgagcgtcg atcgcgcaat cgatgctgcg cacaggctcg ccaaggccgc cgacatcgac
     5041 ggtacgcccg ccttcatcat caacggaaag attcgcgaag gcgcaatgaa cgacgacgac
     5101 ttcgcgaaaa tgacgaagac gtgattctca gatcagcccc gcaagcggac tcgacggatc
     5161 ggcataacgt ttcttcgcca tccggccggc gagataggcg agcctgcccg cttcgacagc
     5221 gtggcgcatc gcgctcgcca tcaatactgg attcctcgct tccgcgatcg ccgtgttcat
     5281 gagcactgcg tcgcagccca gctccatcgc cacggtcgcg tctgatgcgg tgccgacacc
     5341 tgcatcgacg atcacgggca ctttggcctc ttccacgatc atgcggatgt tgactgcgtt
     5401 ctgcacgccg agaccggagc cgatcggcgc ggcgaggggc atgatcgcga cagcacccat
     5461 gtgttcgaga cgcctggcca tcagcggatc gtcgctgcaa tagaccatca cctggaatcc
     5521 ttcgcgaatc agcagttccg tggcgcgcag agtctccagc atgtcgggat agagcgtctt
     5581 gcggtcgccc aggacttcga gcttcacaag tgtccagccg ccggcctcgc gcgcgagccg
     5641 caacgtacgc acggcctctt cgccggtgaa gcatcctgcc gtattgggca ggtaggtgac
     5701 ttttttcgga tcgatgtagt cgacaagccg cggtgcgcca ggatcgctca gattcacccg
     5761 tcgcacggca acggtcacga tttcggctcc cgacgcctcg agcgccgcgg cgttctccgc
     5821 ataggtcttg tacttgccgg ttccgatgat cagtcgcgac gcgtaggtcc ttccggcgat
     5881 tgcgagcggc ttgccctcgg gcaggccggc gattgcagcg ttaccgccgc cgatgaagtg
     5941 cacgatctcg atcctgtctc cgtcgcccag cctgatgtcg gcgtagctcg agcgcgggac
     6001 gatttccagg ttgcgctcga cagcgatctt ggcaggatcg aggccgagat gaatcagcaa
     6061 ttcccgcacg gtaagcaagc ggtcgaacct tcgttcttcg ccgttgatgt tgagagccaa
     6121 cgccatgcgc ccttgcctta tccaggtttc cgtcgccgcc aatggacagt ataaaacccg
     6181 ctgatcgcgc ttcgaccgga gatttcgtgg cccagtcccg caaacgctct ctgccgatat
     6241 atgtcctcaa cggaccaaat ctcaatctgc ttggcagccg tgagcccgcg atctacggca
     6301 aggccacgct ccaacagatc ggcgaagcca ccgccgcccg ggcgcgcagc ttggactggg
     6361 aaatcgtttt ccggcaaagc aaccgcgaag gcgagctcat cgactgggtt caggaagcgc
     6421 gaaatggcgc acgtggcatc atcctcaacg ccgggggata cagccacaca tcgatcgcat
     6481 tgctcgacgc ctgccgcgcg gtcgagatac ctcttgtgga ggtccatctc tccaatcctt
     6541 accggcgcga aacctttcgc cgccattcct atataagtga agctgccgac ggcgtgatct
     6601 gcggttttgg ggccgccggc tacctgttgg ccgtcgatgc actggctgaa ttgctcaagg
     6661 cccgatcatg aacatgaagg acatgaagca gctgggcgcc cggctggcga ctggcggaaa
     6721 ggcgaaaccg ccgtttgggg tcgacgccgg cgccattcga gaactcgcgg aactgctgga
     6781 tgaaaccggg ctcgcggaaa tcgagatcga acagggcggc tgccgcatcc gggttgcccg
     6841 ccaggcggct gcggcaccca ccggctctca cgcgccgctg cccgccatgt ccgagccacc
     6901 tggtgcgcca gggcaggcgc cactcgaagg cccagcggcg aagcgcgacg gcgcgatttc
     6961 gtcgcctatg gtcggaaccg tgtatgtcgc gccggagccc ggcaagcctc cctttgtcaa
     7021 gatcggggat acagtccgcg aaggcgacac cctgctgatt gtcgaagcca tgaagacgat
     7081 gaatccgatt atcgccccgc gctctggaat cgtgaaggag atttgtgtcg aagacgcgca
     7141 gccgatcgag ttcgggcaaa cccttctcgt tctttcctga gattttggtt gaagacggtt
     7201 tggaggcgcg cacacggcca aacatgtttg aaaagattct catagccaat cgcggcgaga
     7261 tcgcgctcag aatcaaccgc gcctgcaagg agatg
//
LOCUS       DASZMC010000365         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4549698,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000365 DASZMC010000000
VERSION     DASZMC010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4549698"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..784
                     /gene="truA"
                     /locus_tag="VG274_07500"
     CDS             47..784
                     /gene="truA"
                     /locus_tag="VG274_07500"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWE06537.1"
                     /translation="MTRFKLTIEYDGSNFVGWQRQDNGASVQGALEEAIFKLSGERVT
                     VTGAGRTDAGVHALGQVAHFDLVKEFTPDKIRDALNHFLRPAPAVVIESRIVDAEFHA
                     RFSAKARHYLYRILTRRAPPALDDGHVWHVVRDLDAEAMHHAAQALVGQHDFTTFRAA
                     ECQAKSPMKTLDRLDVARVGEEVHINASARSFLHNQVRSMVGSLKLAGEGKWSRRDME
                     RALKACDRSECGPVAPPDGLYLVRVDY"
     gene            complement(778..1587)
                     /gene="murI"
                     /locus_tag="VG274_07505"
     CDS             complement(778..1587)
                     /gene="murI"
                     /locus_tag="VG274_07505"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWE06538.1"
                     /translation="MSDDAIGIFDSGMGGLTVLRALMARLPNERFIYLGDTARLPYGT
                     KSPDTVTRYAVQAAGALVRRRIKLLVIACNTASGVALGALQEAFAPLPVIDVIGPGAN
                     AALTASPSGPIAIIATEATVKAQAYVRAIHALAPDVAVTQQACPLFVSLAEEGLVEGE
                     IAELVAQRYLAPVLAQKPHAMVLGCTHYPALKATIGKIAGRGVKLVDSAETTAEEVER
                     RLKTLNLLRRSGDAPVHHFLATDAPERFERVGEIFLGQKIDPASIELVDLQ"
     gene            complement(1584..>2315)
                     /gene="dapE"
                     /locus_tag="VG274_07510"
     CDS             complement(1584..>2315)
                     /gene="dapE"
                     /locus_tag="VG274_07510"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF009557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HWE06539.1"
                     /translation="NGTPKMLGWLKEHGETLSHCIVGEPTATHYAGDTLKIGRRGSFT
                     GKITVLGTQGHVGYPQNALNPIPVLAALISRLSSHVLDEGTEHFQPSTLSFTTVDVGN
                     PATNVIPAEAHATFNIRFNDSHSPESLQDWIAREMEQVLPPSFRIYVTPTVGGDVFLT
                     KPGTFTELISVSVEAATGSRPLFSTTGGTSDARFIRSHCPVAELGLPGGTMHKIDECV
                     PVAEIARLSDIYLAILEHYFADPPQ"
BASE COUNT          432 a          708 c          724 g          451 t
ORIGIN      
        1 atgatgtatt gcgcggtttc gcgctgccga aaggcacaat cctttcgtga cccgtttcaa
       61 gctcaccatc gaatatgacg gcagcaactt tgtcggctgg cagcggcagg acaatggcgc
      121 gtcggtgcag ggcgcgctgg aagaggcgat cttcaagctc tccggtgagc gtgtcaccgt
      181 caccggcgcg ggccgaacgg atgccggcgt gcatgcgctg ggtcaggttg cgcatttcga
      241 tctggtgaag gaattcacgc cagacaagat ccgcgacgcg ctcaatcatt ttctgcggcc
      301 tgcgcctgcg gtcgtgatcg agtcgcggat cgtggatgcg gaattccatg cgcgcttctc
      361 ggccaaggcg cggcattatc tctatcgcat cctcacgcgc cgcgcgccgc ccgcgctcga
      421 tgatggtcat gtctggcatg tcgtgcgcga tctcgatgcc gaggcgatgc atcacgccgc
      481 acaggcgctg gtcggccagc acgatttcac caccttccgc gccgcggagt gtcaggcgaa
      541 gtcaccgatg aaaacgctcg accgcctcga tgtcgcgcgt gttggcgagg aggttcatat
      601 caacgcgagc gcgcgctcct tcctgcacaa tcaggtacgc tccatggtcg gaagcctgaa
      661 gctggccggc gaaggcaagt ggtcgcgccg cgatatggaa cgcgcgctca aggcctgcga
      721 ccgcagcgaa tgcggccccg tcgcgccgcc ggatgggctt tatcttgtgc gcgtggatta
      781 ttgaagatcg acgagttcga tcgacgccgg atcgatcttc tggcccagga aaatttcccc
      841 cacgcgttcg aagcgttccg gcgcatcggt ggcgaggaaa tggtgcaccg gcgcatcgcc
      901 tgaacggcgc agcaagttca gcgttttcag cctgcgttcg acttcctcgg ctgtcgtctc
      961 cgcgctgtcg accagcttca cgccgcgacc cgcaatcttg ccgatggtcg ccttcagcgc
     1021 cggataatgc gtgcagccga gcaccatggc gtgcggcttc tgcgctagca cgggcgcgag
     1081 ataacgctgc gccacaagct ccgcgatctc gccttcgacc agaccttctt ccgccagtga
     1141 cacgaacagc ggacaggcct gctgcgtcac cgcaacatcc ggcgccagcg catggatcgc
     1201 acggacatag gcctgtgctt tcacggttgc ttccgtggcg atgatcgcga taggaccgga
     1261 tggcgatgcg gtcagtgccg cgttcgcgcc gggaccgatc acatcgatca ccggcaacgg
     1321 tgcgaacgcc tcttgcaatg cgccaagcgc gacaccagat gccgtgttgc aggcgatgac
     1381 gagcagcttg atccgccgcc gcaccagcgc acccgccgcc tgcacggcat agcgcgtcac
     1441 cgtgtcggga ctcttggtgc catagggcag ccgcgcggtg tcgccaagat agatgaagcg
     1501 ttcgttcggc agccgcgcca tcagcgcgcg cagcaccgta agcccgccca tgccggagtc
     1561 gaagatgccg atggcgtcgt cgctcattgc ggcggatcgg cgaagtaatg ttcgaggatc
     1621 gctagataga tgtcgctcag ccgcgcgatc tccgcgaccg gaacgcactc gtcgatctta
     1681 tgcatcgtgc cgccgggaag cccaagctct gcgaccggac aatgcgaacg gataaaccgc
     1741 gcatccgacg tgccgccggt ggtggagaaa aggggcctgg aaccggttgc agcttcgact
     1801 gacaccgaaa ttagttcggt gaatgtaccg ggtttggtga gaaacacgtc gccaccgacg
     1861 gtgggagtaa cgtaaatccg aaagctaggc ggcaggactt gctccatctc tctcgcgatc
     1921 cagtcctgca gggactctgg gctgtgggag tcgttgaagc gtatgttaaa cgtcgcgtgc
     1981 gcttccgcag gtatcacatt cgtcgccgga tttccgacat cgacagtcgt gaacgatagg
     2041 gttgatggct gaaaatgctc ggtgccttcg tcgagaacat gagaggatag tctcgatatc
     2101 aacgcagcca gcactggaat cgggttaagg gcattctgcg gatagccaac atgaccttga
     2161 gtgccgagca ccgtgatctt tccggtgaat gatcctcgac ggccaatttt gagcgtatcg
     2221 cccgcatagt gtgttgccgt cggttcgccg acgatgcaat ggctgagcgt ctcgccatgc
     2281 tctttcagcc agccgagcat cttcggcgtg ccgtt
//
LOCUS       DASZMC010000366         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2074426,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000366 DASZMC010000000
VERSION     DASZMC010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074426"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VG274_07515"
     CDS             complement(<1..251)
                     /locus_tag="VG274_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06540.1"
                     /translation="MFNYSDERVKPKRGERENRPGEGLDGIFRGREGKRHLFYRERRL
                     ERWAGLRGVHRGWAEIVRGRRKKMDGRRIEPVGDSAAHV"
     gene            329..706
                     /locus_tag="VG274_07520"
     CDS             329..706
                     /locus_tag="VG274_07520"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HWE06541.1"
                     /translation="MDIPARDWQELTRLTQGAALIVERVRLASGGIAIEGAFVPPQLA
                     QLSAEDQVFVAAFVRAHGSIKEMEQVFAVSYPTIKARLNRIAASLEFVDTNPRPSRGQ
                     VIERLQRGEISASDAIAALEALK"
     gene            802..1224
                     /locus_tag="VG274_07525"
     CDS             802..1224
                     /locus_tag="VG274_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06542.1"
                     /translation="MNEHRRQILDMLASGKITADEAERLLAALESTPGHAAGDARPSP
                     RYLHVVVDKNGDGDVRGKKVDIRVPLQLLRAGVRLASVLPGDARERVNEALHRKGVAF
                     DVGNLKPENVDALIEQLNDISFNIDNARGHATVKMYCE"
     gene            complement(1257..1493)
                     /locus_tag="VG274_07530"
     CDS             complement(1257..1493)
                     /locus_tag="VG274_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06543.1"
                     /translation="MSVQITVRDVPEKVRDELASRAALQGKSMQEFLRAELERLAARP
                     TIDAWLKQVHRRKRAARIRVSAAQILASRDADRR"
     gene            complement(1571..1936)
                     /locus_tag="VG274_07535"
     CDS             complement(1571..1936)
                     /locus_tag="VG274_07535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWE06544.1"
                     /translation="MKKPIARSDLSFAPFEVVVVPFPCTDRLAEKRRPAIVISAPGLQ
                     KRHGLVWLAMVTSAANPAWHFDVMIADLESAALPAPSRVRPAKIATVDAARILHRIGR
                     LSRTDATAVRTNLRRLAAV"
     gene            complement(1911..2132)
                     /locus_tag="VG274_07540"
     CDS             complement(1911..2132)
                     /locus_tag="VG274_07540"
                     /inference="COORDINATES: protein motif:HMM:NF027267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HWE06545.1"
                     /translation="MNAPLSRLTSRAQTVIPRAVREKLGLKPGDLVRYVFEGRRVVIE
                     RAKTEADDDPFATFNEWAGDADEKAYRSL"
BASE COUNT          405 a          681 c          691 g          409 t
ORIGIN      
        1 tatacatgcg cagcagaatc cccgaccggt tcgattcgac gcccgtccat ttttttgcgg
       61 cgtcctcgaa caatttccgc ccatccgcgg tgaacaccac gaagcccggc ccatcgttcc
      121 agccgtcttt cgcggtaaaa aagatgtcgt ttcccttcgc gccctcgaaa tatcccgtcc
      181 aggccttcgc cgggacgatt ttctcgttcg ccgcgtttgg gtttgacccg ttcgtccgaa
      241 taattgaaca ttgttgagtg ttgtgcttgc aagcatcaat ttttatgata tcttactcaa
      301 gataattgat agatgacgga aaaatacctt ggacatcccg gcccgtgact ggcaggaact
      361 gacgcgtctt acccaaggcg ctgcactgat cgtcgagcgg gtgcggctcg cgtccggcgg
      421 catcgcgatc gaaggcgcct tcgtgccgcc gcagctcgcg caactgtccg ccgaggacca
      481 ggtgttcgtc gccgcctttg tgcgcgctca cggttcgatc aaggagatgg agcaggtctt
      541 cgccgtcagc tatccgacca tcaaggcgcg gctgaaccgc atcgccgcca gcctcgaatt
      601 cgtcgacacc aacccgcgcc cgtcgcgcgg acaggtgatc gagcggctgc agcggggcga
      661 gatctctgcg tccgacgcca tcgccgcgct ggaggcgctc aaatgattcc gccgttccat
      721 cgccgtgcgc ctgcgtgcga cgcatatgaa aatcatcggt cctgcaattc cctcttcatg
      781 ccgatctgac tggagaatgc gatgaacgaa caccgacggc agattctgga catgctcgcg
      841 tccgggaaaa tcacggccga cgaagccgag cgcctcctcg ccgcgcttga gagcacgccc
      901 gggcacgcgg ccggcgacgc caggccgtcg ccgcgctatc tgcacgtcgt ggtggacaag
      961 aatggtgacg gcgatgtgcg gggcaaaaag gtcgatatcc gggtgccgct gcaattgctg
     1021 cgcgccggcg tgcgcctcgc cagcgtgctg ccgggcgacg cgcgcgaacg ggtgaacgag
     1081 gctttgcaca ggaagggcgt ggcgttcgat gtgggcaatc tgaaacccga aaatgtcgac
     1141 gcgctgatcg agcagctgaa cgacatctcc ttcaatatcg acaatgcgag aggccacgca
     1201 acggtgaaga tgtactgcga gtgagcgttg ggcggaaata cagaacacga caacgctcaa
     1261 cggcggtccg cgtcacggct ggcgagaatc tgcgcggccg agacgcggat ccgggccgcc
     1321 cgctttcgcc tgtgcacctg tttcagccat gcgtcgatgg tagggcgggc cgccaggcgc
     1381 tccagctccg cacggaggaa ctcctgcatc gatttgccct gcaaggcggc gcgcgaagcc
     1441 agctcgtcgc ggaccttttc ggggacatcg cggacggtga tctgcacgct catgctgtca
     1501 taacgccagc aatgaaggca gagcgcaagc agatgccgga tccgcggtgc gggggcgcgg
     1561 cgccagcggc tcacactgcc gccagtctcc gcagatttgt tctgacggcc gttgcatccg
     1621 tcctggacag ccgtccgatg cggtggagaa ttcgcgccgc atcgacggtg gcgatcttgg
     1681 cggggcgaac gcgtgacggc gcgggcagcg cggccgattc gagatcggca atcatcacat
     1741 cgaaatgcca cgccgggttg gcggcgcttg taaccatcgc aagccagacg agcccgtgac
     1801 gcttttgcag cccgggcgcg ctgatcacga tggcgggccg gcgcttttcc gccagcctgt
     1861 ccgtacaggg aaacggaacg acgacgacct cgaagggcgc gaagctcagg tcagagcgag
     1921 cgataggctt tttcatcggc gtcgccggcc cattcgttga acgtcgcgaa gggatcgtcg
     1981 tcggcttcgg ttttcgcgcg ctcgatcacg acgcgcctgc cttcgaacac gtaacgcacg
     2041 aggtcgccgg gcttcaggcc cagcttctcg cgtacggccc tggggatgac ggtctgagcc
     2101 ctggaggtga gccgggatag cggcgcgttc attcctgttc cctggcgggc tgctgctgca
     2161 aagtaagaat gtaagacgtc ttactt
//
LOCUS       DASZMC010000367         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5100162,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000367 DASZMC010000000
VERSION     DASZMC010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5100162"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /gene="treZ"
                     /locus_tag="VG274_07545"
     CDS             complement(<1..1590)
                     /gene="treZ"
                     /locus_tag="VG274_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HWE06546.1"
                     /translation="MNSVTWRSSFGANLIGPKRTRFRIWAPAQKTMSVAFEGGDRVVM
                     EPAGDGWFEAEAACGAGTRYRFVPPDGTAIPDPASRAQDSDVHSASIVVDPTSYHWRH
                     PQWMGRPWHETVLYELHAGVLDGYAGIARQLQRLAELGVTAIELMPIAEFPGARNWGY
                     DGTLPYAPEHSYGTPDELRALIDAAHGHGLMVFLDVVYNHFGPDGNYLPILAPEMFRK
                     DVSTPWGPAIDFRRPEVRRFFTENALYWLLDYRFDGLRFDAVHAISEGDWLDEMAAEV
                     RRAVGSERHVHLVLENDRNDAAYLAHAFDAQWNDDAHHVLHVLLTGEREGYYEDYIDK
                     PAEQLARCLRDGFIYQGQPSRHRGGTPRGSPSAALPPTAFVLFLQNHDQIGNRAFGER
                     LTTLADPRALEAATALQLLAPQIPLIFMGEETASEAPFLFFTDHNPELAKAVREGRKA
                     EFSTFFQFSDPALVARLPDPNARDTFERSNPAPQRENAAERTQLYRRLLAIRREEVVP
                     RLRNARARAAQPLGSAAVLARW"
     gene            complement(1587..>1881)
                     /locus_tag="VG274_07550"
     CDS             complement(1587..>1881)
                     /locus_tag="VG274_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06547.1"
                     /translation="SDGKEMADQSWKDPNRRLLCLRRASTNEKGGLELLSFLLNPTAE
                     DEFFILPKPALPGRILIDTTQPDSDEIPLTDIKVAVRSHGAVLVYSKPERPLQ"
BASE COUNT          380 a          559 c          602 g          340 t
ORIGIN      
        1 ccagcgcgcc aggaccgcgg ccgagccgag tggctgggcc gcgcgcgcgc gggcatttcg
       61 cagccgcggc acgacctctt cgcggcgaat ggccagaagc cgacgataaa gctgcgttcg
      121 ctcggcggca ttttcacgct gcggggcggg atttgaacgt tcaaatgtat cgcgcgcatt
      181 cggatcgggc aggcgggcga caagcgccgg atcggagaac tggaagaagg tagaaaattc
      241 cgccttgcgt ccttcgcgaa ccgctttcgc aagctccgga ttgtgatcgg tgaaaaacaa
      301 aaatggtgcc tcgctcgccg tctcctcgcc catgaaaatg agcggaatct gtggggcgag
      361 aagttgcagc gctgtggcgg cttctagcgc gcgagggtcg gcgagcgtcg tcagccgctc
      421 gccgaacgcg cgattgccga tctgatcgtg gttctgcagg aacagaacga aagccgtcgg
      481 cggcagagcc gcactggggg agccgcgtgg cgtgccgcca cgatgccgcg agggttgacc
      541 ttggtagatg aaaccgtccc gcaggcagcg ggccagctgt tcggcgggct tgtcgatata
      601 atcctcgtaa tagccctcgc gctcaccggt caaaagcaca tgcagcacgt ggtgcgcatc
      661 gtcgttccac tgcgcatcga aggcgtgtgc cagataggca gcgtcgtttc tatcgttttc
      721 aagaaccaag tgcacgtgac gctcggaccc gacagcgcgg cgaacttccg cagccatttc
      781 gtcgagccaa tctccttcgg aaatggcatg aacagcgtca aatcggagcc catcgaaacg
      841 gtaatcgagc agccaataga gggcgttttc ggtaaagaac cggcggacct ccgggcggcg
      901 aaaatcgatg gccgggcccc acggcgtgga aacgtccttg cgaaacattt cgggcgccag
      961 gatcggcaga taattcccgt ccgggccgaa gtgattgtat acaacgtcga ggaagaccat
     1021 gaggccgtgc ccgtgcgcgg cgtcgatcaa tgcacgcaac tcatcgggcg tgccgtagct
     1081 gtgctcaggc gcatagggca gagtgccgtc gtagccccag ttgcgcgcgc ctggaaactc
     1141 cgcgattggc atcaattcga tcgccgtaac gccaagctcg gcgagccgct gcaattgccg
     1201 ggcaatgccg gcgtagccgt ccagaactcc ggcatgaagc tcgtacaaca ccgtctcatg
     1261 ccagggccgc cccatccatt gtggatgacg ccaatgatac gacgtgggat ccaccacgat
     1321 gctggcgcta tggacgtcgc tgtcctgtgc tcgcgaagca gggtcgggga tcgcggtgcc
     1381 gtccggcggc acgaagcgat agcgcgtacc ggctccgcag gcggcttcgg cctcgaacca
     1441 tccgtcaccg gcaggctcca tgacaacgcg gtctccgcct tcgaatgcaa cagacatcgt
     1501 cttttgcgcg ggagcccaga tgcgaaatcg cgtccgcttc ggtccaatca ggttggcgcc
     1561 gaacgacgag cgccaagtaa cgctattcac tgcaatggtc tttccggttt ggaataaacc
     1621 aacacggcgc cgtgcgaacg gacggcgacc ttgatgtcgg tgaggggaat ttcatccgag
     1681 tcgggttgcg tagtgtcgat caggatgcgg ccaggaagcg ccggcttggg caaaatgaaa
     1741 aactcatcct ctgccgtcgg gttgagcaga aaactcagta attccagccc gcccttttcg
     1801 ttagtcgagg cgcggcgcag gcagagcaag cggcgattgg ggtccttcca ggattgatcg
     1861 gccatttcct tgccgtcgct a
//
LOCUS       DASZMC010000368         4538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3180563,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000368 DASZMC010000000
VERSION     DASZMC010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180563"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /gene="gluQRS"
                     /locus_tag="VG274_07555"
     CDS             complement(<1..623)
                     /gene="gluQRS"
                     /locus_tag="VG274_07555"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HWE06548.1"
                     /translation="MAALASYLDAREHGGEWMVRIEDLDEPRCVPGADADILRTLERF
                     GLEWDGPVVYQSARLEAYRAALERLQCGGHVYPCGCSRPEAGEIYAGKCRGRAPQPGK
                     PVAWRVGAQDVTICFDDRRAGTYCQNLACDVGDFVVLRADGMFAYQLAVVVDDAEQGI
                     TDVVRGADLLDSTPRQIWLQRLLGYAQPRYMHVPVVTNAAGEKLSKQT"
     gene            715..1725
                     /locus_tag="VG274_07560"
     CDS             715..1725
                     /locus_tag="VG274_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWE06549.1"
                     /translation="MKDIARELGVSVVTVSKALRNHDDISEETRGKVLRLMRDLNYRP
                     NLAARALVTGRSFIVGFVVPGLLHSFFAEIAKGLTNVFRKHGYGLVIATSEEDPELES
                     QEIEQLLARRVDALIVASTQTSPATFARIDEQGIPYVLIDRRVEQVSANFVGVDDRFV
                     GLVATEHLIQCGCRRIAYIYGSRLSPALGRLDGYRRALEKHRLPVRPEYIRALDETGD
                     ASDDATYAAMKELLALDEPPDGVFCFNDPGALNAMKAVLEAGLRIPEDVAVVGCGNIR
                     HADFLRVPLTSIDQNSVAIGERAGKLALGLIESKTPQTPKTILLEPRLVVRHSSMRAT
                     AP"
     gene            1804..3648
                     /locus_tag="VG274_07565"
     CDS             1804..3648
                     /locus_tag="VG274_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE06550.1"
                     /translation="MKLLSGSKAGIVRELLRPHLLTLSLALLAAIGESFADLLSPWPL
                     KVVIDNVIKTKPLPPWVSHIVVALAGTDRLAIVRVAAIGAIVIAVLGAACAYIQKRGA
                     TTVGQWVMHDLRRTMYSHIQRLSLKFYDESHTGDLISRVTSDIDTIQSFITSDLLDAI
                     IQSLTLVGMIAWMLYLDWRFTLVSLSVAPVLFAVVFRYTRIIKKSSREVRKKEGEIVS
                     TIQEVITSIRVVKAFAREDYEQQRLEEQSLESVEIGLRARGIKARLAPMVEIIVAAGT
                     SLVLWFGAREVLAGTLSAGSLVLFFFYLGKMYKPMQGLSKMSDSYSKASVAFERIRET
                     LDMHGDVRDLPRAMRAPRLRGLIEFEHVTFGYDPARPVCSDLNFRIEPGQVAALVGPT
                     GAGKTTIVSLIPRFYDPISGAVKIDGLDIRRFKIRSLREQMSFVLQETVLFHAPVWQN
                     IAYGKPTASREEILSAAREANAAEFIEKMPEGYNTIVGERGVTLSGGQRQRIAIARAI
                     IRNTPILILDEPTSGLDAESERLVVQALERLMRGRTTIVIAHRLSTIRRADQIFVVCD
                     GWIAEHGRHDELRAAGGIYAKLYETQFGLADDAQSAPADHPHASAIEI"
     gene            complement(3604..>4538)
                     /locus_tag="VG274_07570"
     CDS             complement(3604..>4538)
                     /locus_tag="VG274_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06551.1"
                     /translation="SWTALRVLVLLALYTAALNSKEMAAIAPILLILYELPSLKLRSY
                     AVPLLLCAITAAAMLGKTQAGGPFHGESMYIASFTLKRFFQNARALQSELFYLHPRDL
                     STRQVLAIWSALLLAPFAIERPHRRALWFSAAFAILGPLPIIFLPGRGFYVMYVPMAG
                     WALYCAMLAVFARDWIAREIRRRLPMRRNGWIAAHAALALCLAYVVTRGLSDPGYTSP
                     HYDPMRKVIAETRSDFASLGEFPPRGARLLLLHSRFPDDAWGPLMIARLLYRDPGLWL
                     DRPAVHLFPAAPDPAAYDLVLDFDGRRMRVVRRR"
BASE COUNT          889 a         1437 c         1405 g          807 t
ORIGIN      
        1 gtctgcttgc tcagtttctc gcccgcggcg ttcgtaacga cgggcacgtg catgtagcgc
       61 ggctgagcgt agccgagcag gcgctgcaac cagatctggc gcggcgtgga atcgagcagg
      121 tcagcgccgc gaaccacatc cgtaattccc tgctccgcgt catcgacaac caccgccagt
      181 tgatacgcaa acattccgtc cgcgcgcagc accacgaaat cgcccacatc gcacgccagg
      241 ttttgacaat acgttcctgc gcgccggtca tcgaagcaga tcgttacatc ctgggcgccg
      301 acgcgccagg ccactggttt acccggctgg ggagcgcgcc cgcggcattt gccggcgtaa
      361 atctcgcccg cctcggggcg cgaacacccg cacggataaa cgtgaccacc gcattgcagc
      421 ctttcaaggg ccgcgcgata cgcctccaaa cgcgcgcttt gatatacgac cggcccgtcc
      481 cactcgagcc cgaagcgttc gagcgttctg aggatatccg cgtccgcgcc ggggacgcaa
      541 cggggttcgt ccagatcctc aatgcgtacc atccattcgc caccgtgctc gcgcgcgtca
      601 agatagctcg ccagcgccgc caccagcgat ccgaaatgca gcggcccggt aggcgatggc
      661 gcgaatctgc ccctgtatcc cacgttacaa tccaagctta tggcagcggt ccggatgaaa
      721 gacatagcgc gcgagctcgg cgtgtctgtg gtgacggttt cgaaggcgct tcgcaatcat
      781 gacgacatca gcgaagagac acgcggcaaa gtcctgcgcc tgatgcggga tctgaactac
      841 cggcccaatc ttgcggcccg cgcgctggtc accggacgct cttttatcgt cggcttcgtg
      901 gttcccggcc tgctgcattc gttttttgcc gagatcgcca aagggctcac caatgttttc
      961 cgcaaacatg gctacggcct ggtgatcgcc acatcggaag aagatccgga actcgagagt
     1021 caggaaatcg aacaactgct cgcgcgccgc gtggacgccc tgatcgtcgc cagcacgcag
     1081 acatcgcccg ccactttcgc gcgcattgac gagcagggga ttccttacgt gctgatcgac
     1141 cgcagggttg agcaggtgag cgcgaatttc gtcggtgtcg acgatcggtt cgtggggctc
     1201 gtggccaccg agcacctgat tcaatgcgga tgccgccgca tcgcatacat atatggatcg
     1261 aggctcagtc cggcgctggg gcgtctggat ggatatcgcc gcgcgctgga aaagcaccgc
     1321 ctgccggttc ggcctgaata catccgcgcg ctggatgaaa cgggcgacgc atcggacgac
     1381 gccacctacg cggccatgaa ggaactgctc gcgctggacg agccgcccga tggcgttttt
     1441 tgcttcaacg accccggcgc gctgaacgcg atgaaggcgg tgctcgaagc ggggctgcgc
     1501 atcccggagg acgtcgcggt ggtcggctgc ggcaatatcc ggcacgccga ctttctgcgc
     1561 gtcccgctga cgtccatcga ccagaacagt gtcgcgatcg gagaacgcgc cggcaagctc
     1621 gctttgggtc tgatcgaatc gaagacgccc cagacgccga aaaccattct gctcgagccg
     1681 cggctggtag tccgccattc cagcatgcgg gcgactgccc cttaaaacgc ggccggtttc
     1741 cagatcgtat tcgttcgctc agtggtagcc aaactgcccc ttcagttgag cgccggtgcc
     1801 aacttgaagc tgctctccgg atccaaagcg ggcattgtcc gcgaactgct gcgtccgcat
     1861 ctgctgactt tgtcgctagc gcttttggcc gctatcggcg aatccttcgc cgacctgctg
     1921 tcgccgtggc cgctgaaagt cgtcatcgac aacgtaatca agacgaagcc gctgcccccg
     1981 tgggtaagcc acattgtagt cgcactcgcg ggcacggacc ggctcgccat tgtacgcgtg
     2041 gcggcaatcg gcgccatcgt gatcgcggtg ctcggagcgg cctgcgccta cattcagaaa
     2101 cgcggcgcca ccacggtcgg ccagtgggtc atgcatgacc tgcgccgcac catgtactcg
     2161 cacatccagc ggctttcgct gaagttttac gacgaatcgc acacgggcga cctgatcagc
     2221 cgtgtcacca gcgacatcga tacaatccag agcttcatca cgtcggatct gctggacgca
     2281 atcattcaga gcctcacgct ggttggcatg atcgcgtgga tgctgtacct cgactggcgc
     2341 ttcaccctgg tctcgctttc cgtcgccccc gtgctctttg cagtcgtctt ccgctacacg
     2401 cgcatcatca agaagtcttc ccgcgaggtt cgtaaaaaag aaggcgagat cgtttccacc
     2461 attcaggaag tcatcacgtc cattcgcgtc gtgaaggcgt tcgcgcgcga ggattacgaa
     2521 cagcaacgcc tcgaagaaca gagtctcgaa agcgtggaga tcggcctcag agcgcgcggc
     2581 atcaaagcgc ggctggcgcc aatggtggaa atcatcgttg ccgccggaac cagccttgtg
     2641 ttgtggttcg gagcccgcga ggttctggcc ggcacgctct cggccggttc attggtgctg
     2701 ttcttcttct atctcggcaa gatgtacaag cccatgcagg ggctttccaa gatgagcgat
     2761 tcgtatagca aggcatccgt cgcctttgaa cggattcgcg aaactctcga catgcacggc
     2821 gatgtacggg acctgccgcg cgccatgcgc gctccccggc tgcgcggcct gattgaattc
     2881 gagcatgtga cgttcggcta cgatccggca cggcccgtct gttccgacct gaatttccgc
     2941 attgaacccg gacaggtcgc ggcgctggtc ggcccgaccg gcgccgggaa gacaaccatc
     3001 gtgagcctga ttccccgttt ctacgacccg atctcgggcg ccgtgaaaat cgacggcctg
     3061 gatatccggc gcttcaaaat ccgctcgttg cgcgagcaga tgagcttcgt gctgcaggag
     3121 accgtgctct tccatgcacc ggtctggcag aacatcgcct acggaaaacc gaccgcgagc
     3181 cgcgaggaaa tcctcagcgc ggcgcgggaa gccaatgccg ccgaattcat cgagaagatg
     3241 cccgagggtt acaacacaat cgtgggcgag cgcggtgtca cgctttcggg cgggcagcgc
     3301 cagcgcatcg ccatcgcgcg cgccatcatc cgcaacaccc cgatcctgat tctggatgag
     3361 ccgacatcgg ggctcgatgc ggaatccgaa cgtctcgtgg tccaggcgct cgagcgcctg
     3421 atgcgcggcc gcaccaccat cgtcatcgca caccgcctct cgaccatccg gcgcgccgat
     3481 cagatattcg tggtctgcga tggctggatc gccgagcacg gcaggcacga tgagcttcgg
     3541 gctgccggcg gcatatacgc caagctctac gagacgcaat tcgggcttgc cgacgatgcg
     3601 cagtcagcgc ctgcggacca cccgcatgcg tctgccatcg aaatctagaa ccaggtcgta
     3661 ggcggcggga tcgggggcag cggggaacag gtgaaccgca ggccggtcaa gccagaggcc
     3721 ggggtcgcga tacaacaggc gggcgatcat gaggggcccc catgcatcgt cgggaaatcg
     3781 agaatgaagg agcaggagtc gcgctccgcg cggcgggaat tccccgagcg aggcaaaatc
     3841 gctccgcgtc tcggcaatga ccttacgcat cggatcgtag tggggagatg tgtagccggg
     3901 gtcggataga ccacgcgtga cgacataggc caggcagagc gcgagcgcgg catgagcggc
     3961 aatccaaccg ttgcggcgca tcggcaaacg gcgccggatc tcgcgggcga tccagtctct
     4021 ggcgaacacc gcgagcatcg cgcaatagag cgcccatccg gccatcggca cgtacatcac
     4081 gtagaacccg cgcccaggca ggaaaattat cggcagcggt ccgagaatcg cgaaggcggc
     4141 gctgaaccac agcgcgcgac gatgggggcg ctcgattgcg aagggcgcga gcagcaacgc
     4201 gctccagatc gcgagaacct ggcgggtact gaggtcgcgc gggtgcaggt agaaaagttc
     4261 agattgcagt gcgcgcgcgt tttgaaagaa gcgtttgagc gtgaacgagg cgatatacat
     4321 cgactcgccg tgaaacggcc cgcccgcctg ggtcttgccc agcatcgcgg ccgcggtgat
     4381 cgcgcagagt aagagcggaa cggcgtagct gcggagttta agcgacggca attcgtagag
     4441 gatcagcaga atcggcgcga tggcggccat ctctttgctg ttcagcgccg ctgtgtagag
     4501 cgccagtaaa acgagaacgc gcaacgctgt ccaggacc
//
LOCUS       DASZMC010000369         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4829345,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000369 DASZMC010000000
VERSION     DASZMC010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4829345"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VG274_07575"
     CDS             <1..378
                     /locus_tag="VG274_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HWE06552.1"
                     /translation="AIVTLLAIAFYFFLATRVAVAHGRYNVKLPAMSGNPDFERICRI
                     HANTLEWMPTFLVPLWLCAVYLSDIGAAVLGLVWIGGRILYFAGYSNAVEKRLPGFFV
                     QSTACFLLFIGAAVGVVMHLAGR"
     gene            484..>1624
                     /locus_tag="VG274_07580"
     CDS             484..>1624
                     /locus_tag="VG274_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWE06553.1"
                     /translation="MTQQREVDLLVAGAGPAGMTAALVASLQGLSVLVCEKSGQVGGT
                     GSTSAGTLWIPGNHQSRDAGFSDSAAQAETYLDALVGNISGRELRTAFLKTGPAAIDW
                     LERNSALKFLPCGRHPDYRSNMKGAAVAGRAIIPEPFDGRLLGADFAKLRPPIPEYML
                     FGGMMIAKADIPPLLDRFKSLANFAHAAKLLVRYALDRLRYPRGTRLTMGNALTARLF
                     YSLKQRGIEILFNTSIAAIDCRDGRVAGATLSTPAGELHVRAKKGVVLATGGYGHNMH
                     YREAFMARPTPVHSMASEFNQGDGIAIGEKLGARIAPQEHRRSGLWTPASVTLRADGS
                     KGLYPHLLLDRAKPGLIAVNARGERFVNEAVSYHDFVEGMFDANAS"
BASE COUNT          272 a          530 c          523 g          299 t
ORIGIN      
        1 gccatcgtca cgctgcttgc gatcgcgttc tattttttcc tcgcgacccg cgttgccgtc
       61 gcgcacggca gatacaacgt caaactgccg gcgatgagcg gcaatcccga tttcgaacgc
      121 atctgccgca tccacgccaa cacgctcgaa tggatgccga cttttctggt gccgctctgg
      181 ctgtgcgcgg tttatctcag cgatattggt gcggcggtcc ttggccttgt ctggatcggg
      241 ggccgcatcc tgtatttcgc cggttacagc aacgccgtgg agaagcggtt gcccggattc
      301 tttgtgcagt cgaccgcgtg cttcctgctc ttcatcggcg cggctgtcgg tgttgtcatg
      361 catctcgcgg gccgctgagc cctcacctca ttgcgagcga aagcttcgtc gctgtcgctc
      421 ctcgcaatga cgggcgcgat ggcgaatgtg ctaacctgca cacaaagggg tggcggtagc
      481 aaaatgacac aacagcgcga ggtcgatctc ctggtggcgg gcgccgggcc ggccggcatg
      541 acggcggcgc tggtggcgag cctgcagggg ctttcggtgc tcgtctgcga gaaatcggga
      601 caggtgggcg gcaccggatc gacctcggcc ggcacgctgt ggatccccgg caatcatcag
      661 agccgcgacg cgggtttttc cgacagcgcc gcgcaagcgg aaacctatct ggacgcgctg
      721 gttggcaaca tttcgggccg cgagctgcgc acggcgtttc tgaaaaccgg gccggctgcg
      781 atcgattggc tggaacgcaa cagcgcgctg aaattcctgc cgtgcgggcg gcatccggac
      841 tatcgcagca acatgaaggg cgctgcggtc gcgggccgcg cgatcattcc ggagccgttc
      901 gacggacggc tccttggcgc ggatttcgcg aagctgcggc cgccgatccc cgaatacatg
      961 ctgttcggcg gcatgatgat cgccaaggcc gacatcccgc cgctgctcga ccgtttcaag
     1021 tcgctggcga atttcgcgca cgcggccaag ctcctcgtgc gctacgcgct cgaccggctg
     1081 cgctatccgc gcggcacgcg cctgaccatg ggcaatgcgc tcactgcccg gctgttctac
     1141 agcctgaagc agcgcggcat cgaaattctt ttcaacacgt cgatcgccgc gatcgactgc
     1201 cgcgacgggc gcgtcgccgg cgcgacgctg agcaccccgg ccggtgagct tcacgtcaga
     1261 gcaaaaaaag gtgtcgtgct ggcgaccggc ggctacggtc acaacatgca ctatcgcgaa
     1321 gccttcatgg cgcggccgac gccggtgcat tcgatggcga gcgaattcaa ccagggcgac
     1381 ggcatcgcca tcggcgaaaa actcggcgcc cgcatcgcgc cgcaggagca tcgccgcagc
     1441 ggactatgga cgcccgcttc cgtcacgctg cgcgccgatg gcagcaaggg gctttatccg
     1501 cacctgctgc tcgatcgcgc caagcctggc ctgatcgccg tcaacgcgcg cggcgagcgc
     1561 ttcgtcaacg aagccgtgtc gtatcacgac tttgtcgaag gcatgttcga cgccaacgcg
     1621 agcg
//
LOCUS       DASZMC010000370         4111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3180833,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000370 DASZMC010000000
VERSION     DASZMC010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180833"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VG274_07585"
     CDS             <1..313
                     /locus_tag="VG274_07585"
                     /inference="COORDINATES: protein motif:HMM:NF037864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06554.1"
                     /translation="CPYQDEELDAIARNCTEREDAARKVQRSMLKRMAAVAMQTRIGQ
                     NYSAIVTGASPKGVYVRISDPPIEGRVIHGDQGLDVGDRVRVSLVGADPQRGFIDFAR
                     A"
     gene            314..787
                     /locus_tag="VG274_07590"
     CDS             314..787
                     /locus_tag="VG274_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06555.1"
                     /translation="MNDIEFLRHSLATLAYRANKALRDAPPDFADFRATAGCRTPAEI
                     VAHMADLFDWALSIVNGVQKWRDSPTLPWGAAVARFFDSLTAFDRRLAAPEPLAASPE
                     RLFQAPVADALTHTGQLALLRRLAGSPVRGENYFVAHIQAGHVGSDQAASVKEFD"
     gene            790..1746
                     /locus_tag="VG274_07595"
     CDS             790..1746
                     /locus_tag="VG274_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HWE06556.1"
                     /translation="MALWLTESDVRALLSLPELIEATERALAAFSAHQIDQPVRTAIE
                     IRDRTFFASMPALDGGHGILGAKLVTVYPDNAARGLHTHLAVISLFDADTGELAAIMD
                     GRYITELRTAAASAVSVRHLARRDASTLAILGSGVQARSHLAALPLVRNFREIRAWSP
                     TPARLAEFARGPVQPAPTAEAAVRGADVILLATSSIKPAIVSEWVDAGAHVIAIGACR
                     PSQREIDPQLVARAALFTDSRAAILKESGDVIQSIREGLFAEDHVLAELGEVVNGSKP
                     GRTSDRQITLFKSLGLAIEDLTAADLVYRRARESGRGLEVSL"
     gene            1749..>4111
                     /locus_tag="VG274_07600"
     CDS             1749..>4111
                     /locus_tag="VG274_07600"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HWE06557.1"
                     /translation="MRAVAVWALALLTLALPLAAQYPTPAAVSDLDALYQSNQWPEFT
                     RALARSPNAPPFYRGVLALAVHDWPQSEKQFQSAMKPGGDPLHSFEAAMGLMRIYELT
                     GRRKDARALLSRLEQTMRSLRGARAIDPAVMRSFRSYRAQMDAAGDYPDQSIAARGLS
                     RVLYAQIENQLIVPLKINGAPANYVIDTGSEQCLLSVSEAHRLGLAVRPLEMGVSGFG
                     PDSITNGITIAPDLVIGNFHLRNVLFLVQRDGDAPAGIIGMPVLLALETIRWSSDGTI
                     EFGFPAAPRAADSNLWFSENLLLTRAELDGKSLMLGLDTGDYESILFPRFSRDFPDTL
                     KPFPLGKREAPRDTSAVALANLRLGGQRIPIAPIPLYAREPIDDATDLHGWLGMDLLS
                     QGRSVTLDFDAMKLTLDGIDRPTAAECPLPSGFHCLPGFDCIVKQPGTAPCFIERIPS
                     TAPAGNPVTSPKSDSPACHLPENAQCAGGALCSVDFDSPQTCHITESSPEPSTGADAR
                     VSAVAENKVPAVPPSAGQPAAGPPDVDVRELLRRSVKYESLDLGPPHDYIYQEEEEKK
                     LLNPDGSVKTVTRTTREVMNLYDAQFTRLIRKDGQDLPPDKARSEQAHFDKAVEKRRR
                     EQASETPQAKAKREETERKRDAENLLCDDEFVRMFDAHISGSEEIAGRPSWIVDLTPR
                     ASAPEPRCHNLKVLTRFSFKLWIDKAEARWARFEGVNIAPVTWMSVLVRVPAQDLHIS
                     FETARHADGLWLQSEMHWRISGKLLLAARFRIDDRTTYSNYRKFQADS"
BASE COUNT          736 a         1561 c         1075 g          739 t
ORIGIN      
        1 ctgcccctat caggacgaag agctcgacgc cattgcgcgc aactgcaccg aaagagaaga
       61 cgccgcccgc aaagtgcagc gcagcatgct caagcgcatg gccgccgtcg ccatgcagac
      121 ccgcatcgga cagaattact cggccatcgt caccggagcc agcccgaaag gcgtctacgt
      181 tcgcatctcc gacccgccca tcgaaggccg cgtcatccac ggcgaccagg gcctcgacgt
      241 aggcgaccgc gtacgcgtct ccctggtcgg cgccgaccca caacgcggtt tcatcgattt
      301 tgcgagggca taagtgaacg acatagaatt tctacgccac tccctagcca cgcttgccta
      361 tcgcgccaac aaggccctgc gcgacgctcc gcccgatttc gccgatttcc gcgccaccgc
      421 cggctgccgc acgccggccg aaatcgttgc gcacatggcc gacctcttcg attgggcgct
      481 ctctatcgtc aacggcgtcc agaaatggcg cgattcccca actctaccct ggggcgccgc
      541 tgtcgcgcgc ttcttcgact cactaacagc cttcgatcgc cgtctagccg cgccggagcc
      601 gcttgccgca tcaccggaaa ggctcttcca ggcgcccgtc gccgacgcgc tcacccacac
      661 cggccagctc gccttgctgc ggcgtctggc cggctcgccc gtgcgcggtg agaactattt
      721 cgttgcccat atccaagccg gccacgtcgg ttccgatcaa gccgcctccg tcaaggaatt
      781 cgactaacga tggcgctttg gctcaccgag tccgatgtcc gcgcgcttct ctctctgccg
      841 gaactcatcg aggccacgga acgcgccctc gccgccttct ccgcgcacca aatcgatcag
      901 cccgtgcgca ccgccatcga aatccgcgac cgcaccttct tcgcctccat gcccgctctc
      961 gatggcggcc acggcatcct cggcgccaag ctggttacgg tctatcccga caacgccgcg
     1021 cgcggcttgc acacgcatct ggcggtcatt tcgctcttcg atgccgatac cggcgagctc
     1081 gccgccataa tggacggccg ctacatcacc gagcttcgca ccgccgccgc atccgccgtt
     1141 tcggtgcgcc atcttgcgcg tcgcgacgca tccaccctcg ccatcctcgg ctccggcgta
     1201 caggcgcgca gccaccttgc cgcgctcccg ctggtccgca actttcgcga aattcgggcc
     1261 tggagcccca cacccgcgcg actggccgaa ttcgcgcgcg gtcccgtgca acccgcgcct
     1321 accgccgaag cagccgtgcg cggggccgat gtaatccttc tcgccaccag ttccatcaaa
     1381 ccggccatcg tcagcgaatg ggtcgacgcc ggcgcgcacg tcatcgccat cggcgcctgc
     1441 cgtccctcgc agcgcgagat cgaccctcaa cttgtcgcgc gcgccgcgct cttcaccgat
     1501 tcccgcgccg ccatcctcaa ggaatccggc gacgtcatcc agtctattcg cgaaggcctc
     1561 ttcgccgaag atcacgtgct cgccgaactc ggcgaagtcg tcaacggaag caagcccgga
     1621 cgcacttccg atcggcagat cactcttttc aagtcgctcg gcctcgcaat tgaagacctc
     1681 accgccgccg atttggtcta tcgtcgcgcg cgtgagtccg gccgcggact cgaagtatcc
     1741 ttataggcat gcgggctgtc gcagtgtggg ctcttgcgtt gctcacgctt gccctgccgc
     1801 tcgcggccca ataccccaca cccgccgccg tctccgacct cgacgccctc taccaatcca
     1861 atcaatggcc cgaattcacc cgggcgctcg cgcgcagccc caacgccccg cccttctatc
     1921 gcggcgtact cgcactcgcc gtgcacgatt ggccgcagtc tgaaaagcaa tttcaatccg
     1981 ctatgaagcc gggcggcgac cccctgcact cgtttgaagc cgccatgggt ctgatgcgca
     2041 tctatgaact caccggccgc cgcaaagatg cccgcgctct gctctcccgc ctcgagcaaa
     2101 ccatgcgctc ccttcgcggc gcgcgcgcca tcgatcccgc ggtcatgcgt tcttttcgaa
     2161 gctaccgtgc ccaaatggac gccgccggcg actaccccga tcaatccatc gccgcccgcg
     2221 ggctttcgcg cgtgctctac gcgcaaatcg aaaatcagtt gatcgtcccg ctgaagatca
     2281 acggcgcgcc cgccaattac gtgatcgaca ccggctccga gcagtgcctc ctgagcgttt
     2341 ccgaggccca ccgcctcggc ctcgccgtgc gaccgctcga aatgggcgtc agcggctttg
     2401 gtcccgattc gatcaccaac ggtatcacca tcgcgcccga cctcgtcatc ggcaactttc
     2461 atctgcgcaa cgtgctcttc ctcgtccagc gcgatggcga tgcgcccgca ggcatcatcg
     2521 gcatgcccgt gctactcgcg ctcgaaacca ttcgctggag ctccgacggc acgatcgaat
     2581 ttggctttcc cgccgcgccg cgcgccgccg attccaatct ctggttcagc gaaaatctgc
     2641 tcctcacgcg cgccgaactc gatggcaaga gcctcatgct cggcctcgac acgggcgatt
     2701 acgaaagcat tctcttcccg cgcttttctc gcgatttccc cgatacgctt aagccgtttc
     2761 cgctcggcaa acgcgaggct cctcgcgata ccagcgctgt cgcgctcgca aacctgcgcc
     2821 tcggcggcca gcgcattccg atcgctccca tccccctcta cgcccgcgag cccatcgacg
     2881 acgccaccga cctgcacggc tggctcggca tggacctcct cagccagggc cgctcggtca
     2941 cgctcgattt tgacgcaatg aaacttactc tagacggcat cgatcgcccc acggccgccg
     3001 agtgcccgct gccgtctggt ttccactgcc tgcccggctt cgactgcatc gtcaagcaac
     3061 ccggaaccgc gccctgcttc atcgagcgca tcccctctac ggcgcctgcc ggtaacccgg
     3121 tcaccagccc caaatccgat tcccctgcct gccaccttcc ggagaacgcg cagtgcgcag
     3181 gcggcgcgct ctgcagcgtc gatttcgatt cgccgcaaac ctgccacatc acggaatctt
     3241 cgcccgagcc ttccacaggg gcagacgctc gcgtttccgc ggtcgccgaa aacaaagtac
     3301 ccgcagtgcc gccttcggcc ggacagcccg ccgccgggcc tcccgatgtc gacgtgcgcg
     3361 aactcctccg ccgctccgtt aaatatgagt cgctggatct tggcccgccc cacgattaca
     3421 tttatcagga agaggaagag aagaagcttc tgaatccgga tggcagcgtt aagaccgtca
     3481 cgcgcacgac gcgcgaagtg atgaatctct acgacgcgca gttcacgcgc ctgatccgca
     3541 aagacggcca ggatctcccg cccgacaaag cccgctccga acaggctcac ttcgacaaag
     3601 ccgtcgaaaa gcgccgccgc gaacaggctt cggaaactcc gcaggccaaa gcgaagcggg
     3661 aagagaccga gcgtaaacgc gacgcggaaa atctactctg cgacgatgaa tttgtccgca
     3721 tgttcgacgc ccacatctcc ggctccgaag agatcgccgg acgcccgtcc tggattgtcg
     3781 acctcacacc tcgcgcgagc gcccccgaac cgcgttgcca caatctcaaa gtactcaccc
     3841 gcttcagttt caagctttgg atcgataagg ccgaagcccg ttgggcgcgc tttgagggcg
     3901 taaacatcgc gcctgtcacc tggatgagcg ttctcgttcg cgtgcccgct caagacctcc
     3961 acatctcttt cgaaaccgcg cgccacgccg atggtctctg gttacaatcg gaaatgcact
     4021 ggcgaatttc tgggaaactt ctgctcgccg cccggtttcg catcgacgac cgcaccactt
     4081 actccaacta ccgtaaattc caagccgatt c
//
LOCUS       DASZMC010000371         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1283696,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000371 DASZMC010000000
VERSION     DASZMC010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283696"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VG274_07605"
     CDS             <1..318
                     /locus_tag="VG274_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06558.1"
                     /translation="HVPARLAAQPVFNEYSMGGYLIFRGIRPFIDGRGDMYGATFAEN
                     YARIIKPDRHAMETTFSKYKVMWTILTPDNAANGILDMLPGWRILYRDKFAIIYVRRD
                     AHV"
     gene            293..1519
                     /locus_tag="VG274_07610"
     CDS             293..1519
                     /locus_tag="VG274_07610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HWE06559.1"
                     /translation="MSAEMHTSEALAPGGPELDERHRRWRTFILWAVAFEFAVVAVLL
                     TRELITLQPPGLDFLPLWTAWGFAWTAPDRIYDFATITHAQAWLIPNWPTPRPFAYPP
                     TALLALAPLGRLPFWPAFGLWTSANLTVFLYAGKQLSSRQRGLAVARTASQPLVVLCL
                     LVGQATLMAAGLVILGILQLDSRPRLSGVLLAVAIAIKPQIALLAPLALFAGGQFKEM
                     AALAIAGAILAALTIVFFGAARWSEWLNCLSAFEAVVRETPRLMSQVIAPNGFAHDFH
                     FSHAATILWNAAFGLGGAIIVWRVFSRDTDATTRAAAILAGGVLMAPYALRFDGAAIV
                     APAVAMAVDRIALPGWTLRLFGLLATVAAAVPYLGGLAVVLFLVIAVAGRASQPGESS
                     APCANGGQEKVTDNIP"
BASE COUNT          280 a          506 c          490 g          360 t
ORIGIN      
        1 cacgttccgg cccggctcgc ggcgcaacct gtattcaatg aatatagcat gggaggttat
       61 ctgatctttc gcggtattcg cccattcatc gacggacgag gagacatgta tggcgccacg
      121 ttcgccgaga attacgcaag aatcattaag cccgacagac acgcgatgga aaccacgttt
      181 tccaaataca aggtcatgtg gaccatattg actcccgaca acgccgcaaa cgggatcctc
      241 gacatgcttc cgggctggcg cattttgtac cgggacaaat ttgcaatcat ttatgtccgc
      301 agagatgcac acgtctgagg cgcttgcgcc cggcggtccc gaactcgacg aacgccatcg
      361 gcgatggcgg acgttcatct tatgggctgt cgctttcgag tttgcagtcg tcgccgttct
      421 gctcacacga gagctcataa cacttcaacc acccggtctc gatttcctgc cgttgtggac
      481 tgcctggggg ttcgcctgga ccgcgccaga tcggatctat gacttcgcga ccattacgca
      541 cgcacaggcc tggctgattc caaactggcc gacgccgagg ccattcgcct atccgccgac
      601 cgcgctgctc gccttggctc cgctgggccg gctgcccttc tggcccgcgt tcggtttatg
      661 gacgtcggca aacctgaccg tctttctcta cgccggcaaa cagctttcga gccggcagcg
      721 tggtcttgcc gtggcgcgca ccgcgagtca gccactggtt gtgctttgcc ttctcgttgg
      781 ccaggcaacg ttgatggcgg cagggctggt aattctcggg attctgcaac tcgattcgcg
      841 tcccagactt tccggcgtcc ttcttgccgt cgccatcgcg atcaagccac agatcgcgct
      901 gctcgcgccg ctcgccctgt tcgccggcgg ccaattcaag gagatggccg cgctcgccat
      961 tgcaggggca attcttgccg ccctgacaat cgttttcttc ggtgcagccc gatggagcga
     1021 gtggctcaat tgcctttccg ctttcgaagc agtcgtgcgt gaaacgcccc gattgatgag
     1081 ccaggtgatc gcgcccaacg ggtttgctca cgatttccac ttctcccacg ccgccaccat
     1141 tctgtggaat gcggcattcg gtctgggagg tgcaatcatc gtctggcgtg ttttctcgcg
     1201 tgacaccgat gcgacgacac gggcggcagc gattctcgcg ggcggtgtcc tgatggcgcc
     1261 atacgcgctg cgatttgacg gggcggcgat cgtagctccg gcggtcgcaa tggctgtgga
     1321 ccggattgcg ctacctggct ggacgctgcg gctttttggg cttcttgcga cagttgctgc
     1381 tgccgtgccc tatcttggcg gattggccgt cgtcctgttc cttgtgatag cggtcgccgg
     1441 acgcgcaagt caaccgggtg agtcgtcggc tccgtgcgca aacggcggac aggagaaagt
     1501 caccgacaac atcccctgac gtgtcacggc ccgcgcaagc gtgccgccca gatgaaacgg
     1561 gctcggaggg gaaagcgaga ggaagaatct tctcgatggc gaaccggaga gcgctcgtgg
     1621 ctgggtggcc cgctct
//
LOCUS       DASZMC010000372         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3181539,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000372 DASZMC010000000
VERSION     DASZMC010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3181539"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..767
                     /locus_tag="VG274_07615"
     CDS             339..767
                     /locus_tag="VG274_07615"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HWE06560.1"
                     /translation="MLAGTLALAVAAIFAGAAYYVSAVEQPARLSLDERAALAEWRPA
                     YKSGTAMQAPLAAIGFLLGALAWWQTRNPAFGVGALAMIANWPWTFLVIMPVNRKLMA
                     DDAARTDPQTRVLLEKWGHLHAVRTGLGLLAAVAFLWALA"
     gene            856..1083
                     /gene="vapB"
                     /locus_tag="VG274_07620"
     CDS             856..1083
                     /gene="vapB"
                     /locus_tag="VG274_07620"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWE06561.1"
                     /translation="MPRTAKIFMNNRSQAVRLPKEFQFDRPEVYIRKQGDDVILSPRP
                     ADWSAYLVEEPAASAEFMKDVEDLPVQERVR"
     gene            1083..1499
                     /locus_tag="VG274_07625"
     CDS             1083..1499
                     /locus_tag="VG274_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE06562.1"
                     /translation="MPRFMLDTDTCSYIVKRSNHAVLRRLQTVPIDEVCISAITEAEL
                     QYGVQVSPRQHTDGVAVGAFLRHIDVLDFSGDAATSYAEIRADLKRRGAMIGAHDLLI
                     AAHARSLDMTLVTNNVREFKRVRGLAIENWAARGTP"
     gene            1557..1925
                     /locus_tag="VG274_07630"
     CDS             1557..1925
                     /locus_tag="VG274_07630"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HWE06563.1"
                     /translation="MSAVEQPARLGLDVRAALAEWLPAYRGGTAMQAPLAAIGFLLGA
                     LAWWRTRNPAFGLGAIAMIANWPWTFLVIMPVNRKLMSDDAARTDPQTRALLEKWGRL
                     HTVRTGLGLLATLAFLWALA"
     gene            2094..2774
                     /locus_tag="VG274_07635"
     CDS             2094..2774
                     /locus_tag="VG274_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HWE06564.1"
                     /translation="MKERSTKTSGRGALRVKVKSAKGRDVSSTRWLARQLNDPYVREA
                     REKGYRSRAAFKLIELDRKFHFLKKGAVVLDLGAAPGGWSQVAAARAGETGHVVAADV
                     LEIAPIAGVEILEADLRDSATSARLAAALRSAADVVLSDMAAPTTGHRPTDHLRTTAL
                     FEAALDVAEQVLKPGGVFLGKVFQGGATGDLLARVKMRFRDVRHVKPPASRAESVELY
                     LVATGFKG"
     gene            2994..>4224
                     /gene="guaB"
                     /locus_tag="VG274_07640"
     CDS             2994..>4224
                     /gene="guaB"
                     /locus_tag="VG274_07640"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWE06565.1"
                     /translation="MPAASEVLPGQVDTRTRLTKTVELGIPVISAAMDTVTEATLAIA
                     MAQAGGMGVIHRNLGIEEQAEHVRRVKKFESGMVVNPVTIAPDATLADALALMERYRI
                     SGIPVVEGATNGAGKLVGIVTNRDVRFATDPRQKVAELMTRDKLVTVREGVDAREAKR
                     LLHQHRIEKILVVDDAYRCVGLITVKDIEKAQKYPNACKDERGRLRVAAATSVGEDGF
                     KRALALVEAECDVIVVDTAHGHSRGVLETVRRIKRESNYTQVAAGNVATADGAKALID
                     AGADAIKVGIGPGSICTTRVVAGVGVPQLTAVMDAVSAARDAGVPVIADGGIRYSGDL
                     AKALAAGAHVAMVGSLLAGTEESPGEVFLYQGRSYKGYRGMGSVGAMARGSADRYFQA
                     EVKDTLKLVPEGVEGQVP"
BASE COUNT          756 a         1305 c         1434 g          729 t
ORIGIN      
        1 gcaaaccacg tgccagctgc gcgcgaacag atgccgccgc tccgcttcga aaaaggccga
       61 gtcggtgtag agtcgggcgg ggaggctctg ttcgggagag agagtcacgc atatctcctg
      121 acgccgacaa aatctatccc agggacgcgg gacatagcca tgcggccgat gcgcattcaa
      181 gattggtgcg tgcgttcacc cttcgacaag ctcagggtga ggagttctgc ctcgtcctga
      241 gcctgtcgaa ggacgacaaa cgtatcccct gggcaacgtg ctccaccgtg aaagctggta
      301 tagccggagt gaacggcatt cgaacggaga tttggaaaat gctggcagga accttggcgc
      361 tcgcggtcgc tgcgatcttt gcgggggctg cgtactatgt gagcgcggtc gaacagccgg
      421 cgcgtctgtc gctcgacgag cgcgcggccc ttgcggaatg gcggcccgcc tacaagagcg
      481 gcacggcgat gcaggcacca ctcgcggcga tcggattcct tctcggcgcc ctcgcctggt
      541 ggcagacgcg caatcctgcc ttcggtgtgg gtgcattggc gatgatcgcc aactggccat
      601 ggacgttcct cgtgatcatg ccggtcaaca ggaagctgat ggccgacgat gcggcgcgca
      661 cggatccgca gacgcgggtg ctgctggaaa aatggggcca cctgcatgca gtccgcaccg
      721 gcctcggcct gctggcggcg gtcgcgttcc tgtgggcgtt ggcgtgatac ggcggtcgga
      781 cagtctggac atgtcgcctg ttgcatagct ggagcgtgtg gcatatactg cagtatatgc
      841 caaagggagc ggcccatgcc cagaaccgcc aaaatcttca tgaacaaccg tagccaggcc
      901 gtgcgtttgc ccaaggaatt tcagttcgac aggccggaag tttacattcg caagcagggc
      961 gacgacgtca ttctgtcacc gcgtcccgct gattggtcgg cctatcttgt ggaggagccg
     1021 gccgcatctg cggagttcat gaaagacgtc gaagatcttc ccgtacagga acgtgtccga
     1081 taatgccccg gttcatgctc gatacggata cgtgttccta catcgtcaag cgctccaatc
     1141 atgcggtgtt gaggcggttg caaacggttc cgatagacga agtgtgcatt tccgcaatca
     1201 ccgaggcgga attgcagtat ggggtacagg tctcgccacg ccagcatacg gacggagtgg
     1261 ccgtgggggc tttccttcgc cacatcgatg ttctcgattt ttccggcgat gccgcgacct
     1321 catacgccga aattcgcgcc gacctgaaac ggcgcggtgc gatgataggc gctcacgatc
     1381 tgctcatcgc cgctcatgcg cgctctttag acatgacgct cgtgacgaac aacgtccgtg
     1441 aattcaaacg cgtccgcggt ctcgccatcg aaaactgggc tgcccgcgga acaccatgaa
     1501 gcagcgctcc gatcgaagct ccggtcgctg cgatctttgc gggggctgcg tactatgtga
     1561 gcgcggtcga acagccggcg cgtctcggac tcgacgtccg cgcggcgctc gcggaatggc
     1621 tgcccgccta caggggcggc accgcgatgc aggcgccgct tgcggcgatc ggattccttc
     1681 tcggcgccct ggcctggtgg cggacgcgca atcctgcgtt cggtctgggc gcaattgcga
     1741 tgatcgcgaa ctggccgtgg acgtttctcg tgatcatgcc ggtcaaccgg aagctgatgt
     1801 ccgacgatgc ggcgcgcacg gatccgcaga cgcgggcgct gctcgaaaaa tggggccgcc
     1861 tgcacacggt tcgcacgggc ctcggcctgc tggcgacgtt ggcattcctt tgggcactgg
     1921 cgtgatgtga cgttggtgaa aaaagctctt aacgcagtgg gcgcagggga tgcgcagggg
     1981 acgcagaaat aaagattgcg cgcctctggt gcgccattcc tgccccgcgg tcctctgcgc
     2041 atcccctgcg tccactgcgt tgaatcttcc ttgtcactcg ttgagatgac gacatgaaag
     2101 aacgttccac caaaacctcc ggccgcgggg ccttgcgggt gaaggtgaag tccgcgaagg
     2161 ggagggatgt gtcatccacc cgctggctcg cgcgccagct caacgatcct tatgtgcgcg
     2221 aggccaggga aaagggctac cgcagccgcg cggcgttcaa gctgatcgag ctggatcgaa
     2281 aatttcattt tctgaagaag ggtgccgtcg tgctcgatct gggcgccgct ccgggcggct
     2341 ggagccaggt ggccgcggcg cgcgcgggcg agacgggaca cgtcgttgct gcggatgttc
     2401 tcgagatcgc gccgatcgcg ggcgtggaga tactcgaagc cgatctgcgc gactccgcaa
     2461 cgtcggcccg gctcgccgcg gcgctccgca gcgcagccga tgttgtcctg tccgacatgg
     2521 cggcgcccac cacgggccac agacccacgg atcatctgcg aaccaccgcg ctgttcgagg
     2581 cagcgctcga cgttgccgaa caggtgctga agccgggcgg cgtgttcctc ggcaaggtgt
     2641 tccagggtgg cgccaccggc gatttgctcg cgcgggtgaa gatgcgcttc cgcgacgtcc
     2701 gccacgtcaa gccgcccgcc agccgcgcgg agtcggtgga gctttatctg gtcgccaccg
     2761 gattcaaggg gtgagcagag aacacggcaa tatcgtgcgt tgtcaggccc cctccgccgg
     2821 cggatcaact ccggggcgga gggggccggc ggatcgaaca acggccacgg ggcgttgagc
     2881 ggggcgcctt tcgccggagg gtccccgctg gtaaaggctc ggcgctccaa ggcaaggccc
     2941 ggagcgacat cctgacgatc cgcgaagcgc tcactttcga cgatgtgctg ctggtgccgg
     3001 cagcttccga agtgctgccc ggccaggtcg atacgcgaac ccgcctcacc aaaaccgtcg
     3061 aactgggcat ccccgtcatc tccgccgcga tggacaccgt caccgaagcc acgctcgcca
     3121 ttgccatggc gcaggcggga gggatgggcg tcatccaccg caatctcggc atcgaagaac
     3181 aggccgagca tgtgcgccgg gtaaagaaat tcgagagcgg catggtggtc aatccggtca
     3241 ccatcgcgcc ggacgccacc ctcgccgacg cgctcgcgct gatggagcgc taccgcatct
     3301 ccggcatccc cgtggtggag ggcgcgacga acggcgcggg caagctcgtc ggcatcgtca
     3361 ccaaccgcga cgtgcgcttc gccaccgatc cgcgccagaa ggtggcggag ctgatgacca
     3421 gggacaagct cgtcaccgtg cgcgaaggcg tcgacgcgcg cgaggccaag cgccttctcc
     3481 accagcatcg catcgagaaa attctggtgg tcgacgacgc ctatcgctgc gtcggcctca
     3541 tcaccgtcaa ggacatcgag aaggcgcaaa aatatcccaa cgcctgcaag gacgaacgcg
     3601 ggcgattgcg cgtcgccgcg gcgacgagcg tcggcgagga cggattcaag cgcgcgctgg
     3661 cgcttgtcga agccgaatgc gacgtgatcg tcgtcgacac cgcgcacggc cattcgcgcg
     3721 gcgtgctcga gacggtgcgg cgcatcaagc gcgaatcgaa ctacacgcag gtggcggccg
     3781 gcaatgtcgc caccgccgac ggcgcgaaag cgctgatcga tgccggcgcg gatgcgatca
     3841 aggtcggcat cgggccgggt tcgatctgca ccacgcgcgt cgtcgcaggc gtgggcgtgc
     3901 cgcagctgac cgcggtgatg gacgcggtat cggcggcgcg cgatgcgggc gtgccggtga
     3961 tcgccgatgg gggcatccgc tattcgggcg atctcgcaaa ggcgctcgcg gcgggcgcgc
     4021 atgtggcgat ggtgggctcg ctgctcgccg gcaccgagga gagccccggc gaggtgtttc
     4081 tctatcaggg ccgcagctac aagggttatc gcggcatggg ttcggtgggc gcgatggcgc
     4141 gaggctctgc ggatcgctac ttccaggcgg aagtgaagga cacgctgaag ctcgtgcccg
     4201 aaggcgtcga gggccaggtg ccgt
//
LOCUS       DASZMC010000373         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5384094,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000373 DASZMC010000000
VERSION     DASZMC010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5384094"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..557)
                     /locus_tag="VG274_07645"
     CDS             complement(225..557)
                     /locus_tag="VG274_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HWE06566.1"
                     /translation="MIARLKAGIYVKALIRRAEIAGASAYVVRRGAEEAGALFLKLAR
                     LDGTCTVLIQARRGEGELVWMKPLGETSDDAAAAKYFEKQVRFDPDLWIVEIEDREGR
                     AFVDEAIV"
     gene            complement(554..2329)
                     /locus_tag="VG274_07650"
     CDS             complement(554..2329)
                     /locus_tag="VG274_07650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HWE06567.1"
                     /translation="MAKVLREMQAPNGKSATSRAQGNAGAIERDLVHARRRFATVQVA
                     KAALGAMLWLAFVALVGKPDMMETLALAGFLAPCVLALLAILGVSLDILESASLACFA
                     ALIGYMAGITGGMHSPLIVWFALVPAEAALAGGRVAVLRAAVSAGLALCAVAAIESLH
                     GLPPSRLVAPVWQIYAGSVLAAIVQSAVIAIAAQDRRRVSDLAAAEGAAMYRFLADHA
                     TDLITRHGSDGRIRFASPAATRLLAVAPEALEGIAPASLVHPDDLKIMQAAFVEASYF
                     DRAATAEVRFRRPDGSVVWTEMRCRPARAMNGKSADIVAVTRDISERKANERALIAAR
                     DMAEQASRAKSRFLANMSHELRTPLNAIIGFSEVMTHQMFGALGTPRYLEYARLIHES
                     GGHLLELINGILDMSKIEAGKFDLSEEIFDLEDAVQQALRFLRLQSERKGVALKMALA
                     PDARMIFGDRRAVKQILVNLLANGVKFTPRGGEVSIVTLRDGSAIEIRVADSGVGIAE
                     ADLRRLGQPFEQGVGSHIGVQEGTGLGLALVRALSAMHGGEAVIDSVLGEGTVVRVRL
                     PHAAVSDADCARHLQETREPLKGAA"
     gene            complement(2694..>2901)
                     /locus_tag="VG274_07655"
     CDS             complement(2694..>2901)
                     /locus_tag="VG274_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06568.1"
                     /translation="EKKDSQGDSEPMRKKWRAIGAQRILTVSLSHCEFFFQVRADRRW
                     QSDKPDPECGPKGHANHHSVGTLW"
BASE COUNT          535 a          955 c          896 g          515 t
ORIGIN      
        1 caaacgtgcc ggtctttggg atccggcgac gcagctcgac cgtcattcat ttcccagtct
       61 gggacaaatc ctgcacgacc agcttgcact gcaacgggac gtggccgagc tcgacgacag
      121 cctgcagaag agcagtctca atctctattg aatcgaagga ccggcaaaat cacgccggga
      181 cggggtgaaa caaaaccccg ccgattgttt tatgaaacaa cccttcacac gatcgcctca
      241 tcgacaaacg cgcggccttc gcggtcttcg atctcgacga tccacaaatc gggatcgaag
      301 cgcacctgtt tctcgaaata tttcgccgcc gccgcgtcgt cgctcgtttc gccgagcggc
      361 ttcatccaca cgagctcgcc ctcgccgcgg cgggcctgga tgagcaccgt gcaggtgccg
      421 tcgagccgcg ccagtttcaa aaacagcgcg cccgcctcct cggctccgcg gcgcaccaca
      481 taggcggagg cgccggcgat ctccgcgcgg cggatcaggg ctttgacgta gatgccggct
      541 ttgaggcgcg caatcacgca gcgcccttaa ggggctcccg cgtttcctgc aagtgccgtg
      601 cgcagtccgc gtcgctgact gccgcatgcg gcagacgcac gcgcaccact gtaccctcgc
      661 ccagcaccga atcgatgacg gcctcgccgc catgcatcgc ggaaagcgcc ctgacgagcg
      721 cgagaccgag accggtacct tcctgcacgc cgatatgcga acccacgccc tgctcgaaag
      781 gctggccgag gcggcgcagg tccgcctctg caatgccgac gcccgaatcg gcgacgcgga
      841 tctcgatcgc gctgccgtcg cgcaacgtga cgatcgacac ttcgccgccc cgcggcgtga
      901 acttgacgcc attggcgagc aggttgacga gaatctgctt caccgcgcgg cggtcgccga
      961 agatcatccg ggcgtccggc gccagcgcca tcttcagcgc cacgcccttg cgttccgatt
     1021 gcagcctgag gaaccgcaac gcctgctgca cggcgtcttc caggtcgaag atctcctcgc
     1081 tcagatcgaa cttgccggcc tcgatcttcg acatgtcgag aatgccgttg atcagctcga
     1141 gcagatggcc gccgctctcg tgaatgagcc gcgcatattc gagatagcgc ggcgtgccca
     1201 gcgcgccgaa catctggtgc gtcatcactt cggaaaagcc gatgatggcg ttgagcgggg
     1261 tgcgcagctc gtggctcata ttggcgagga agcgcgattt cgcccggctc gcctgttcgg
     1321 ccatgtcgcg cgcggcaatg agggcgcgct cgttcgcctt gcgctcggaa atgtcgcgtg
     1381 tcaccgccac gatatcggcg ctcttgccgt tcatggcgcg cgccggacgg cagcgcatct
     1441 ccgtccacac cacgctaccg tcgggccgcc ggaagcgcac ttccgccgtc gccgcgcggt
     1501 cgaaataact cgcttcgacg aaggccgcct gcatgatttt cagatcgtcg ggatggacga
     1561 gcgaggcggg cgcaatgcct tccagcgcct ccggcgcgac ggcgagcagc cgcgtggcgg
     1621 cgggactcgc aaagcggatg cggccgtccg atccgtgccg cgtgatcagg tcggtggcgt
     1681 ggtcggcgag gaagcggtac atggccgcgc cttccgccgc ggcgagatcg gaaacgcggc
     1741 ggcggtcctg cgcggcaatc gcgatgacgg cggattgcac gatcgcggca agcaccgagc
     1801 cggcgtagat ctgccacacc ggcgccacga gccgcgacgg cggcaatccg tgcagcgatt
     1861 cgatcgcggc gaccgcgcag agcgccaatc ccgccgacac ggcagcgcgc aggacggcaa
     1921 cgcgtccgcc cgcgagtgcg gcttccgccg gcaccagcgc gaaccacacg atgagcggcg
     1981 agtgcatgcc gcccgtaatt ccggccatgt agccgatcag cgcggcgaag caggccaatg
     2041 atgcggattc gagaatgtcg agagacacac cgaggattgc gagcagcgca agcacgcagg
     2101 gcgcgaggaa cccggcgagc gcgagggttt ccatcatatc cggcttgccg accagcgcga
     2161 cgaaggcgag ccacagcatg gcgccgagcg ccgcttttgc gacctggacc gttgcgaagc
     2221 gccgccgcgc atgcacgaga tcgcgctcga tcgcacccgc gtttccttgc gcgcgactcg
     2281 ttgccgattt gccgttcggg gcttgcatct cgcgcagcac cttggccacg caaacttaac
     2341 gaagtttgaa tcgccgcagt tttcgttcgt taatccgcaa gttttttgat gcagaaagat
     2401 tcatcgattt ttaaccatgc ggacgattcg cacggattgc gggccgcgcg cggctcccgt
     2461 catgttctcg acgcgcttcc agccgttacg cgacgatgtt ttgcgtggga ttccatccac
     2521 agccattttt tcgcaaatcc ggttagtccc gacgaacttc caggacgacg ctcgatcgaa
     2581 tcggaatgta gcggaacggg taaccggatt ttgaccagcg aggtcgtccg aacacgttaa
     2641 cgcgttgcgt tggattttga ggcgtagaca gaaaacgtct cgaaacgggg cggctaccag
     2701 agggtcccaa cggaatgatg attcgcgtgc cctttcgggc cgcattccgg atcgggcttg
     2761 tcgctctgcc aacgacggtc cgcgcggacc tggaagaaaa attcacaatg agacagtgag
     2821 acggtgagaa ttctttgcgc gcctatggcg cgccatttct tcctcattgg ctcactgtct
     2881 ccctgtgaat ctttcttttc t
//
LOCUS       DASZMC010000374         5472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4006784,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000374 DASZMC010000000
VERSION     DASZMC010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4006784"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VG274_07660"
     CDS             <1..944
                     /locus_tag="VG274_07660"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWE06569.1"
                     /translation="FADTVFFGNSGVEAIECALKMARRFHYARGEGERFRTITFEGAF
                     HGRTLAAIAAGGQPKYLEGFGPKAEGFDQVPFGDLRAVEAAVAPTSAAILIEPIQGEG
                     GLSPATPEFLRALRRLCDERGLLLILDEVQTGVGRTGKLFAYEWSGVEPDIMAIAKGI
                     GGGFPLGACLATERAASGMTAGSHASTFGGNPLATAVGNAVLDVVLAPEFLPRVERVA
                     NFARQQLAALPSEYPHIFEDVRGQGLMLGLKIKVPNTGFIDELRERKLLAVAAGGNVV
                     RLLPPLIVEEDHVREAVRILGEAAAAFPARASEPEAA"
     gene            941..1888
                     /gene="argF"
                     /locus_tag="VG274_07665"
     CDS             941..1888
                     /gene="argF"
                     /locus_tag="VG274_07665"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWE06570.1"
                     /translation="MNALTRTAATPRHFVDLADLDTQTLEGLIGEARRRKAARQGLPH
                     GAADPDKPLEGRVLALIFDKHSTRTRISFDIAMRQLGGTTLMMSGAEMQLAREETIAD
                     TARVLSRYLDAVMIRLLDHEMVRELADNADIPVINGLTKMSHPCQVMADVMTFEERKG
                     PIRGRTVAWTGDANNVLASWIHAAARFGFRLRIATPRELAPREGLHDWAMGAGAAVEF
                     GADPERAVAGADCVVTDAWVSMGDEDAVKRHNLLRAYQVNERLMSRAAKDAIFMHCLP
                     AHRGEEVTNEVIDGPQSAVFDEAENRLHAQKAILAWCLA"
     gene            1876..2817
                     /locus_tag="VG274_07670"
     CDS             1876..2817
                     /locus_tag="VG274_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HWE06571.1"
                     /translation="MSRVSVAERDYRRAPAADFVLPFDLPQAGLRGRLVRLDAVSTRA
                     LEAQVLPEPAARVAAEICVLGTLLGSALKLDGRLTVQTKSAGPLDLVAADYYGAEAGR
                     PAGVRSFARLDEERFSSLGEVPPSFEAMAGEGVVAITIEPRQGDQRYQGIVSLAPQSI
                     AATAEAYFAQSEQLPTAIRLAAAPVYVPGKPGSHWLAGGLMLQATPEGRVDAYDWERL
                     AAFLATVEDMELVDVSLAAETLLWRLFHDDEVRVHPAETIAFRCDCESGRIASVLRSY
                     PPSQREGLSDPDGIIRARCEFCGRVHEVAPLDLQQPN"
     gene            complement(2744..3574)
                     /locus_tag="VG274_07675"
     CDS             complement(2744..3574)
                     /locus_tag="VG274_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWE06572.1"
                     /translation="MGRTDPYFRGFDLKLPDFYREREFRQFVAHRNLPNLSLVRFMTD
                     HTGDFAHALDGIDTPEAQVADNDYAVGRLVEAVSRSPYRDSTLIFVVEDDAQDGPDHV
                     DAHRSTAFVVGPFVRQGRVVSARFTTVNMLRTIEEVLGIQPLSIYEAAQRPMADVFDL
                     KQKKWDFSARPSQALRASALPIPKRRANSRDDSGFAFAHDAAWWARKTAGYDWSSEDR
                     IDAEQYDRLLWTGLAGARPYPSAPRGPRTERKPALSSAAGGPAARLRGHARRTRNAHG
                     "
     gene            3835..5274
                     /locus_tag="VG274_07680"
     CDS             3835..5274
                     /locus_tag="VG274_07680"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWE06573.1"
                     /translation="MLAYEELQGSRGREIRYRPPRYQARTLFPNTPPRLRVGAKAYQI
                     HNVSLGGVAAIAKEARDDELSVGDAVRLTIQQSGLPIFESDAKVRWVEKTIFGSKIAF
                     GFVDSFLEIDKLINRNLQAQIATRSSLARSVALVPQDYRSFCADVLTLLRGYRAVLDA
                     NLSVPPEFTRNFDHAGVFEACEARLVQEWRTLWRTGNDIVRGLMGERERRDAVKEYTE
                     LALTPELRGGAIWDRSYAKPMGYPGDFGIMNQVYDWQRVGSNAYDMLLHRLGLEVAEC
                     IKTRMEVVRADIGDAVAAKGAERTTRILSLGCGSAREVETWLARSRAAPPRVEFTLID
                     QEQQALDYAYRAAYPHLVRDEGRCRINCLHMSFTDVLRGTSGLHTLPPQDLIYSVGLI
                     DYLTDRRAASLVRRLYDLVAPGGLLIIGNMNEMPQSNLWPMEFITDWSLYYRNEAQML
                     AWTEGLDPAGVRTTTEPTGRVRLLYVAKR"
     gene            complement(5276..>5472)
                     /locus_tag="VG274_07685"
     CDS             complement(5276..>5472)
                     /locus_tag="VG274_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWE06574.1"
                     /translation="VEGALSRRYGGTGLGLPFAKKIAELHGGTLTLESEVDKGTRIEI
                     RMPSSRVIARAYPQPVRQAV"
BASE COUNT          944 a         1730 c         1788 g         1010 t
ORIGIN      
        1 cattcgccga caccgttttt ttcggcaatt cgggggtgga agcgatcgag tgcgcgctca
       61 agatggcgcg gcgatttcat tacgcgcgcg gagaaggcga gcgctttcgc acgatcacct
      121 tcgagggcgc gtttcacggc cgcacgctgg cggcgatcgc ggccggcggg cagcccaaat
      181 acctcgaagg ctttggcccg aaagcggaag gtttcgacca ggtgccgttc ggcgatcttc
      241 gtgcggtaga ggcagcggtc gcaccaactt cagccgcgat cctgatcgaa cccatccagg
      301 gcgaaggcgg actgtcacct gcaacgccgg agtttttgcg cgcgttgcgc cgtctttgcg
      361 acgagcgcgg cctgcttctc atcctcgacg aagtgcagac cggcgtcggc cgcaccggaa
      421 agctcttcgc ttacgaatgg tccggcgtgg aaccggacat catggcgatc gccaagggca
      481 tcggcggcgg gttcccgcta ggcgcctgcc ttgccacgga gcgcgctgcg tccggcatga
      541 cggccggctc gcatgccagc acctttggcg gcaatccgct tgccacggcc gtcggcaatg
      601 ccgtcctcga tgtcgtgctc gcgccggagt tcctgccgcg tgtcgagcgc gtcgccaact
      661 ttgcgcgcca gcagctcgct gctcttcctt cggagtatcc ccatatcttc gaagacgtac
      721 gcggacaggg cctgatgctg ggactgaaga tcaaggtgcc gaacaccggg ttcatcgacg
      781 agttgcggga gcggaagctg cttgcggtcg cggcgggcgg caatgtcgtg cggctgcttc
      841 ctccgctgat cgtcgaggaa gatcacgtcc gcgaagccgt gcgcattctc ggcgaggcgg
      901 ccgccgcctt tcccgcgcgg gccagcgagc cggaggcggc atgaacgcgc tcacgcgaac
      961 cgcggcaaca cccagacatt ttgtcgatct tgccgacctc gatacccaga cgctcgaagg
     1021 gctgatcggc gaggcacggc ggcgcaaggc tgcgcgccag ggtctgccgc acggtgcggc
     1081 cgatcccgac aagccgctcg aaggccgcgt gctggcgctc atcttcgaca agcattcgac
     1141 gcgcacccgc atctcgttcg acatcgcgat gcggcagctg ggcgggacga cgctgatgat
     1201 gtccggtgca gaaatgcagc tcgcccgcga ggagacgatt gcagacacgg cgcgagtgct
     1261 atcccgctac ctcgacgcgg tgatgatccg tctgctcgat catgagatgg tccgcgagct
     1321 cgccgacaat gccgatattc ccgtcatcaa cggtctgacg aaaatgtccc atccctgcca
     1381 ggtgatggcg gacgtgatga cgttcgagga acgaaaggga cccatacgcg ggcggacggt
     1441 cgcctggacg ggagacgcca acaacgttct tgcgtcatgg attcatgccg cggcgcggtt
     1501 tggattccgc ctcaggatcg ccacgcccag ggagcttgca cctcgcgagg gtcttcacga
     1561 ttgggcgatg ggcgcgggcg ccgccgtcga gttcggcgcc gatcccgaac gggcggtcgc
     1621 cggcgcggac tgcgtcgtca ccgacgcgtg ggtttcgatg ggcgatgaag acgccgtcaa
     1681 gcgccacaat ctgctgcgcg cctatcaggt caacgagagg ctgatgtcgc gcgcggccaa
     1741 agacgcgatc ttcatgcatt gcctgcctgc gcatcgcggc gaggaggtga cgaatgaagt
     1801 catcgacggt ccgcaatcgg cggtgttcga cgaagcggaa aaccggctgc acgcgcagaa
     1861 ggccattctc gcctggtgtc tcgcgtgagc gtggccgagc gggactaccg gcgcgcgccg
     1921 gcagccgatt tcgttttgcc tttcgatctg ccgcaggcgg gcctgcgcgg tcgattggtc
     1981 aggcttgatg ccgtctcgac gcgggcgctc gaggctcaag ttctgcccga gcctgcggcg
     2041 cgcgtcgccg cggagatttg cgtcctcggc acgctcctcg gttcggcgct caagctcgac
     2101 gggcggctga ccgttcagac gaagagcgcc gggccgctgg atctggttgc cgccgattat
     2161 tacggcgcgg aggccggccg gcccgcgggc gtgcgctcct tcgcgcggct cgacgaagag
     2221 cgcttttcct ctctgggcga agttccgccc tcgttcgagg ccatggcggg cgagggagtc
     2281 gttgcgatca ccatcgagcc cagacagggc gaccagcgct atcagggcat cgtctccctg
     2341 gctccgcaaa gcatagcggc cactgcggag gcttatttcg cgcagtcgga acaattgccg
     2401 acggcgatcc ggctcgccgc cgcgcccgtc tatgtgccgg ggaagcccgg ctcgcactgg
     2461 ctggccggcg ggctcatgct gcaggccacg cccgagggcc gcgtcgacgc gtatgattgg
     2521 gaacgcctgg cggcgtttct ggcgacggtc gaggacatgg agctggtcga tgtctcgctg
     2581 gcggcagaga cgcttctatg gcgcctgttc catgacgacg aagtgcgcgt gcatccggcc
     2641 gaaaccatcg ccttccgatg cgattgcgag tccggacgca tcgcgtcggt gctgcgctcc
     2701 tatccgcctt cccagcgcga gggtctgtcg gatcccgacg gaatcatccg tgcgcgttgc
     2761 gagttctgcg ggcgtgtcca cgaagtcgcg ccgctggacc tccagcagcc gaactgagcg
     2821 ccggttttct ctcagttcga ggcccgcgtg gggcgctcgg atacggccgc gcgccggcaa
     2881 gcccggtcca gagcagccga tcgtactgtt cggcatcgat ccggtcttcg ctgctccagt
     2941 cgtaaccggc cgtcttccgc gcccaccaag ccgcgtcatg ggcaaaagcg aatccggaat
     3001 cgtccctcga attggcgcga cgtttcggga tcggcagcgc gctcgctcgc aaggcctgcg
     3061 atggccgggc cgagaaatcc cacttcttct gcttgagatc gaaaacatct gccatcggcc
     3121 gctgagcggc ttcgtagatg gacaaaggct ggatgccgag gacctcttcg atcgtgcgca
     3181 gcatgttcac cgtcgtaaaa cgtgcagaca cgacccgacc ttgccgcaca aatggaccca
     3241 cgacgaaagc cgtgcttcga tgtgcgtcga cgtgatccgg tccgtcctgc gcgtcatcct
     3301 cgacgacaaa gatcagcgtg gaatcgcggt agggactcct tgaaaccgct tcgacgaggc
     3361 gccccaccgc gtaatcattg tcggccacct gcgcttccgg cgtgtcgatg ccgtcgagcg
     3421 cgtgagcgaa atcgccggtg tggtcggtca tgaagcggac cagcgagagg ttcggcaggt
     3481 ttctgtgcgc gacgaattgc cggaattcgc gctcccggta gaagtccggc agcttgaggt
     3541 cgaagccgcg aaaatatgga tccgttcggc ccatcagcgc cgtgtcggct gatcacggaa
     3601 gtttctcccc ggcggatggt ggtctcaaac gatgtccttt ccgtcagggt cgtgtcgatt
     3661 atcgaaagat cataattttt cttgcttttc gcgcagtttg atttattaac aagtcattaa
     3721 gaattcagtc cggcgatttg ggcggtaaga cccggcgacc ggaagtgggg ctgaagagca
     3781 tttgtgtgtc ggcaacgggg gcgcaaaggc gagatccttt gcgaaggggg cagtatgctt
     3841 gcttatgagg agttgcaggg ctcgcggggc cgggaaatcc ggtatcggcc accgcgctac
     3901 caggcgcgca cgctgtttcc caacacgcct cccaggctcc gcgtgggcgc gaaagcctac
     3961 cagatccaca atgtgagcct tggcggcgtc gcggcgatcg ccaaggaagc ccgcgacgac
     4021 gagttgagcg tcggcgatgc agtcagactg accatccagc aatcgggcct gccgatcttc
     4081 gaatcggacg cgaaagtccg ctgggtcgaa aaaacaatct tcggttcgaa aatcgccttc
     4141 ggcttcgtcg acagttttct cgagatcgac aagctcatca atcgcaatct ccaggcccag
     4201 atcgccacgc gatcttcgct cgcgcgctct gtggccctcg tgccgcagga ttaccgctcc
     4261 ttctgcgcgg acgtattgac gctgctcaga ggctatcgcg cggtgctcga cgcgaatctg
     4321 tcggtgccgc cggaattcac ccgcaatttc gaccatgcag gcgtgttcga agcctgcgag
     4381 gcgcggcttg tgcaggaatg gcgcactctg tggcggacgg gcaacgacat cgtgcgcggc
     4441 ctgatgggag agcgcgagag acgcgacgcg gtgaaggaat acaccgaact tgctctgacg
     4501 ccggagcttc gggggggcgc catctgggac cgttcctatg ccaagcccat gggctatccc
     4561 ggcgacttcg gcatcatgaa tcaggtctat gactggcagc gtgtcggcag caatgcctac
     4621 gacatgctcc tgcatcggct gggccttgaa gttgccgaat gcatcaagac acgcatggaa
     4681 gtcgtgcgcg ccgacatcgg cgatgcggtc gccgccaaag gcgcagagcg caccacgcga
     4741 atcctcagcc tggggtgcgg ctcggcgcgc gaagtcgaga cctggctcgc caggagccgc
     4801 gccgcgccgc ctcgcgtgga attcaccctg atcgatcagg aacaacaggc gctcgactat
     4861 gcctaccgcg ccgcctatcc ccatcttgtc cgcgacgaag gccgttgccg gatcaactgt
     4921 ctgcacatgt cgttcaccga tgtgctgcgg ggaaccagcg gattgcatac gcttccgccg
     4981 caggacctga tctattcggt gggcctcatc gattatctca ccgaccggcg tgcggcgagc
     5041 ctggtgcggc gtctttacga tctggtggcg cccggcgggc tgctgatcat cggaaacatg
     5101 aacgaaatgc cgcagagcaa tctctggccg atggaattca tcaccgactg gtcgctgtac
     5161 taccgcaacg aagcccagat gctggcctgg accgaaggtc tcgatccggc cggcgtgcgc
     5221 acgacgaccg agcccaccgg acgtgtgcgc cttctctacg tcgccaagcg ctgaatcaga
     5281 cggcttgtct cacaggttgc gggtaagcgc gggcgatcac gcggctcgag ggcatccgga
     5341 tctctatgcg cgtgcctttg tcgacttcgc tttcgagcgt gagcgtgccg ccatggagct
     5401 ccgctatttt cttggcaaac ggcaggccga ggcccgtgcc gccatagcgg cgcgacaacg
     5461 cgccctcgac ct
//
LOCUS       DASZMC010000375         4144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2629956,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000375 DASZMC010000000
VERSION     DASZMC010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629956"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="trpS"
                     /locus_tag="VG274_07690"
     CDS             complement(<1..601)
                     /gene="trpS"
                     /locus_tag="VG274_07690"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWE06575.1"
                     /translation="MTAPSHRPLVLSGMQPTNTLHLGNYLGALKNWVRLQHDMDALYC
                     VVDMHALTQESGYGHPREIAQATREVTAAYIASGVDPARAVLFNQSQVHEHAELAWIF
                     NCVARLGWLDRMTQFKEKSGKHKERASVGLYTYPVLMAADILAYKATHVPVGEDQKQH
                     LELARDIAQKFNGDFDAPDFFPLPEPVITGTATRVMSLRD"
     gene            complement(820..3555)
                     /locus_tag="VG274_07695"
     CDS             complement(820..3555)
                     /locus_tag="VG274_07695"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF003467.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HWE06576.1"
                     /translation="MGYQTPAQTDLLDAAALRRDLTALAHAEPDQERRRKAALAHIKP
                     VFQDARMRINTRIENGGLPGMAAARALCGAQDVVIQVLYDFATKHFYYAQNPTESERL
                     AIVATGGYGRGLLAPCSDVDLLFLRPFKQTAWGESVIELILLMLWDLGLKVGHATRSL
                     GECIRLAREDMTIRTSLLETRFVWGEAPLWQGLRQRYFAEIAGGNGPEFVEAKLAERE
                     ARHRKQGESRYLVEPNIKEGKGGLRDLQTLYWMGKYLYRVDDPADLVDHAVFTREEYR
                     IFHAAEAFLWDVRCQLHCAAGRAQERLTFDIQPELARRLGFADASPRRAVERFMHAYF
                     LVAKDVGDLTRIFCAALEEQNRKKRPALSRLLPGFLKARAPGDHFFVENGRLNAREDA
                     FARDPCNLIRIFHLADVKDVDVHPQALRTITRSLHLITGELRENRTANRLFLEILTSR
                     RDPERALRRMNEAGVLGRFVPAFSHAVALMQFNMYHHYTVDEHLIRAVGILAAIERGT
                     FRRDNPLASELIGRVKSRETLYCAVLLHDIAKGLEGNHSETGAAIVQQLCPRLGLSDA
                     DTAATVWLVRNHLLMSDTAQRRDISDPETVRHFVSTVQSPELLRLLLVLTVADIRAVG
                     PGVWNGWKAQLLRDLYHEADAVMSGASATPSRAARIAEARDALSRRLADWPEEARTDA
                     LSCHHDNYWLAFDDAELERHARLRRRAKERRDLIALDARPDAARDVGDIAVCTPDRAG
                     LFSQIAGAIAVCGGSIVEAKAFTTDDGFALDVFAVQDGEGRPFAEPRRVARLHQTIAA
                     MLKDDAPHLAAIVRRRRRSDAFRVRPRVSFDNEASAAASVIEVECADRPGLLFDVTRA
                     IYEQQLSISSAIVATYGERAVDVFYVRDEYGHKVTNAERIASIQSHLLRVLANEK"
     gene            complement(3616..>4144)
                     /gene="aqpZ"
                     /locus_tag="VG274_07700"
     CDS             complement(3616..>4144)
                     /gene="aqpZ"
                     /locus_tag="VG274_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HWE06577.1"
                     /translation="NPAISLGLCTARRFPIRDLVPYWIAQVAGGIAGAAIVYAIASGT
                     AGFDASNGFASNGYGEHSPQGYTLLACLICEIVMTFMFLMVVLGATSPKAPFGFAPLA
                     IGLALTLIHLISIPVTNTSVNPARSTAVALFQGSWAVGQLWAFWLAPFLGAILAGLVH
                     RWLAPGDPPSIIPEM"
BASE COUNT          756 a         1311 c         1338 g          739 t
ORIGIN      
        1 cgtcgcgcaa cgacatgacg cgtgtcgctg tccccgtaat cacgggttcc ggcagcggaa
       61 agaaatccgg cgcatcgaaa tcgccgttga acttctgtgc gatgtcgcgg gcaagttcga
      121 gatgctgctt ctggtcctcg cccacgggca catgcgtggc cttgtaggcg agaatgtcgg
      181 ctgccatcag aacgggatag gtgtacagcc cgaccgaagc gcgctccttg tgcttgccgg
      241 acttctcctt gaactgcgtc atgcggtcga gccagcccag ccgcgccacg caattgaaga
      301 tccacgcgag ctccgcgtgc tcatggacct gactctggtt gaagagaacg gctctggccg
      361 gatcgacgcc cgacgcaatg taggccgctg ttacttcgcg cgttgcctgc gcgatctccc
      421 taggatggcc atagccggat tcctgtgtca gcgcgtgcat gtcgacgacg cagtagagcg
      481 catccatgtc gtgctgaagc cgcacccagt tcttgagcgc gcccaggtaa ttgccgagat
      541 gcagcgtgtt ggtgggctgc atgccggaga ggacgagcgg cctgtgcgat ggcgcggtca
      601 tgagcgggtc cgttgcgggt cgcgccttat cgccgagcgg gagaggcggt tcaaggattc
      661 aatttcggct gatgtttcat ccaacctccc ccttgcgggg aggtccaaaa cgcccttcgc
      721 gtttttcggg cgggggtaac gacgcaacgc acagaccccc acccgaaatc gcgtgccgcg
      781 atttcgacct ctccacaagg gggaggtcag aaccacggtt catttctcat tggcaagaac
      841 tcgcagcaga tgggattgaa tggacgcgat gcgctcggcg ttggtgacct tgtggccgta
      901 ttcgtcgcgg acatagaaaa cgtcgacggc gcgttcgcca taggtcgcga cgatcgccga
      961 cgagatcgac agttgctgtt cgtagatcgc gcgcgtaacg tcgaagagca ggccgggccg
     1021 gtccgcacat tccacttcaa tgacgctggc ggcggccgac gcctcgttgt cgaaactcac
     1081 gcgcggcctg acccggaacg cgtcggatcg ccttctgcgt ctgacgatcg cagccaggtg
     1141 cggcgcatcg tccttcagca ttgccgcgat cgtctggtgc agccgcgcaa cccggcgggg
     1201 ctccgcaaat ggtctgcctt cgccgtcctg caccgcgaac acgtcgagcg cgaaaccatc
     1261 gtcggttgtg aacgccttgg cctcgacaat cgagcccccg cagacggcga tggctccggc
     1321 gatctgcgag aacagtccgg cgcgatcggg agtgcagact gcaatatctc cgacatcgcg
     1381 cgccgcgtcg ggtctggcat cgagcgcgat cagatcgcgt ctctccttcg cccgccgtct
     1441 cagccgcgcg tgccgttcga gctcggcgtc gtcgaaggcg agccagtaat tgtcgtggtg
     1501 gcaggaaagc gcatcggtgc gcgcctcttc gggccagtcg gcgagacgcc gcgacagcgc
     1561 atccctggct tcggcgatgc gcgccgcgcg tgaaggggtc gccgacgcac ccgacatgac
     1621 cgcgtccgcc tcatgataca gatcgcgcag cagctgcgcc ttccagccat tccagacgcc
     1681 cggtccgacc gcgcggatat ccgcgacggt gagtacgagc aggagccgca gcagctcggg
     1741 cgattgcacg gtggaaacga aatgccgcac ggtctccgga tcggagatgt cgcgccgctg
     1801 cgcagtgtcg ctcatcagca gatggttgcg caccagccag accgttgccg cagtgtcggc
     1861 gtccgaaagg ccgagacgcg gacaaagctg ctggacgatt gcagcacccg tttcggaatg
     1921 attgccctcc aggcccttgg cgatgtcgtg cagaagaacg gcgcagtaga gcgtttcgcg
     1981 ggatttgacg cggccaatca gttcgctggc gagcggattg tcccgcctga acgtgccgcg
     2041 ctcgatcgcc gccagaattc caaccgcgcg tatgagatgc tcgtcgacag tgtagtggtg
     2101 atacatgttg aactgcatga gcgcgacggc gtggctgaac gccggcacga aacggcccag
     2161 cacgccggcc tcgttcattc ttctgagcgc ccgttcggga tcgcgccgcg aggtgagaat
     2221 ttcgaggaac agccggttcg ccgtccggtt ctcgcgcaat tcgccggtaa tgagatgtag
     2281 cgaacgcgtg atcgtgcgca gcgcctgggg atggacgtcg acatccttca cgtcggcgag
     2341 gtgaaaaatc cttatcagat tgcacggatc gcgcgcgaag gcatcctcgc gcgcgttaag
     2401 ccgcccgttc tcgacgaaga aatggtcgcc cggggcgcgg gctttcagga agccgggcag
     2461 caggcgtgac agcgcaggtc tcttcttcct gttctgctct tccagcgctg cgcagaagat
     2521 gcgggtgagg tcgcccacgt ccttggccac cagaaaatag gcatgcatga agcgctcgac
     2581 ggcgcggcgc gggctcgcat ccgcaaagcc gagccgtcgc gccagctccg gctggatgtc
     2641 gaaagtcagc cgttcctgcg cgcggccggc agcgcagtgg agctggcagc gcacgtccca
     2701 cagaaacgcc tccgccgcgt gaaaaatcct gtattcctcg cgcgtaaaca ctgcgtgatc
     2761 gaccaggtcc gcgggatcgt cgacgcgata gagatatttt cccatccagt agagcgtctg
     2821 caggtcgcga agcccgccct tgccctcctt gatgttcggc tcgacgaggt agcggctctc
     2881 gccctgcttc ctgtggcgcg cttcgcgctc ggcaagtttg gcttcgacga attccggtcc
     2941 gttcccgccg gcgatttcgg cgaagtagcg ctggcgcaag ccctgccaaa ggggtgcctc
     3001 gccccacacg aagcgcgtct ccagcagcga ggtgcgaatc gtcatgtcct cacgggcaag
     3061 ccggatgcat tcgccgagag atcgcgttgc gtggcccacc ttgaggccca gatcccacag
     3121 catgagcagg atgagttcga tcacgctttc gccccacgcc gtctgtttga aggggcgcaa
     3181 aaagaggaga tcgacgtccg aacagggcgc gagcagtccg cggccgtaac cgcccgtcgc
     3241 aacgattgcg agacgttccg attccgtcgg gttttgtgcg taatagaaat gcttggtggc
     3301 gaaatcgtac agaacctgga tgaccacgtc ctgtgcgccg cacagcgcgc gcgccgccgc
     3361 cattcccggc aagccgccgt tctcgatccg cgtgttgatg cgcatcctgg cgtcctgaaa
     3421 gacaggtttt atgtgggcca gcgcggcctt gcggcgccgt tcctggtcag gctcggcgtg
     3481 ggcgagcgca gtcaggtccc gccgcagcgc cgctgcgtcg agaagatccg tctgcgcggg
     3541 cgtctggtaa cccaatgcct cttccgtccg accgggcggt catcaaacgg cccgctttcg
     3601 cccaaggcaa gccgcctaca tttctggaat gatgctcgga gggtcgcccg gcgccagcca
     3661 gcgatggacg aggccggcga gaattgcgcc caaaaacggc gcgagccaga acgcccagag
     3721 ctgtccgacc gcccagctgc cctggaacag cgcgaccgcg gtcgagcgcg ccgggttcac
     3781 ggacgtgttg gtgaccggaa tcgagatcag atggatcagc gtgagtgcga gcccgatggc
     3841 gagcggcgcg aatccaaatg gcgccttcgg cgatgtcgca ccaaggacga ccatgagaaa
     3901 catgaacgtc atcacgatct cgcagatgag acaggcaagc agcgtgtagc cctgcggcga
     3961 atgttcgcca tagccattac tagcaaatcc gttcgaggcg tcgaaccccg ccgtgccgct
     4021 cgcgatggcg tagacgatag ccgcgccggc gatgccgcct gcgacctgcg cgatccagta
     4081 gggaacgagg tcacgaatcg gaaaccgcct cgcggtgcag agcccaaggc tgattgccgg
     4141 attg
//
LOCUS       DASZMC010000376         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3462576,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000376 DASZMC010000000
VERSION     DASZMC010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462576"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VG274_07705"
     CDS             complement(<1..1221)
                     /locus_tag="VG274_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWE06578.1"
                     /translation="MSPLFRKLLIANRGEIAIRVVRTARHLGIATVAVYSDADARAAH
                     VEAADEAVRIGAAAPRESYLNIDAVISAARKSAADAIHPGYGFLSENAEFAEACASAG
                     IAFVGPPPDAIRAMGLKDRAKKLMAKAGVAVVPGYLGAEQSLPKLSHEAGRIGFPVLI
                     KAVAGGGGKGMRRVDAPDEFEGALEGAKREAQSAFGDDRVLIEKYVTRPRHIEVQIFA
                     DTQGNAVHLFERDCSLQRRHQKVIEEATAPGMSQPMRAAMGAAAVAAAKAVDYVGAGT
                     VEFIADASEGLKADRFWFMEMNTRLQVEHPVTEMISGLDLVEWQLRVAAGEPLPWKQD
                     ELHARGHAVEARLYAEDPQSGFLPSVGQLERLRLPEAGDGVRVDTGVRENDLVTMYYD
                     PMIAKVIAWDEVREI"
     gene            complement(1228..2835)
                     /locus_tag="VG274_07710"
     CDS             complement(1228..2835)
                     /locus_tag="VG274_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWE06579.1"
                     /translation="MSVIKTAIATREAAFKANAAAMAALVAELKSRMNLAAQGGDERS
                     RKRHTDRGKFLPRERVERLVDPGSPFLEFSPLAAFGMYDGDIHAAGIITGIGRIDGRE
                     CMILCNDATIKGGTYYPVTVKKHLRAQEIARENRLPCVYLVDSGGANLPNQAEIFPDR
                     DHFGRIFYNQANLSALGIPQIAAVMGSCTAGGAYVPAMSDETIIVKGQGTIFLGGPPL
                     VKAATGEIVSAEDLGGAEVHARVSGVADHYANDDSHALAILRRIVANLNATKSPDIAC
                     AEPKPPLYDIAELDGIVPQSLSIQYDVHEVIARLVDGSVFDEFKRLYAPTIVTGFAHI
                     EGMPVGIVANNGILFSESALKTAHFIELCCQRRVPLLFLQNIAGFMVGRKYETGGIAK
                     DGAKMVTAVACAAVPKITVLIGGSYGAGNYGMCGRAYSPRFLFSWPNSRISVMGGEQA
                     ASVLATVKRDGMESRGESWSAADEDAFKAPIRARYEEEGNPYYATARLWDDGMIAPAD
                     TRRVLALALSACLNAPIPDSRFGVFRM"
     gene            complement(2832..>3243)
                     /locus_tag="VG274_07715"
     CDS             complement(2832..>3243)
                     /locus_tag="VG274_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWE06580.1"
                     /translation="VVAREDFAARRGLKPIARIVAQASHAAEPRWFTTAPVGAIGKVL
                     DASGWRKEDVDLFEINEAFACVAMIAMRDLGLPHERVNVNGGACALGHPIGASGARIV
                     VTLLNALEKRGLKRGVAALCIGGGEATAVAVERL"
BASE COUNT          593 a         1083 c          971 g          596 t
ORIGIN      
        1 gatttcgcga acttcgtccc aggcgatcac cttggcgatc atcggatcgt agtacatggt
       61 cacgagatcg ttctcgcgca ccccggtatc gacccgcacg ccgtcgcccg cttccggaag
      121 tcgcagccgc tcaagctggc caacggaagg caggaagccg ctctgcggat cttcggcgta
      181 aagtcgcgcc tcgacggcgt gtccccgcgc atgaagttca tcctgtttcc aggggagcgg
      241 ttcgcctgcc gccacccgca attgccactc cacaagatcg agaccgctga tcatttcggt
      301 aacgggatgc tcgacctgca atcgcgtgtt catttccatg aaccagaagc gatcggcctt
      361 caggccttcc gatgcatcgg caatgaattc gaccgtgcct gcaccgacat aatccaccgc
      421 tttggcggcc gctacggcgg ccgcgcccat cgccgctctc atcggttggc tcatccccgg
      481 cgccgtggcc tcttcgatca ccttctggtg ccggcgttgc aacgagcagt cgcgctcgaa
      541 cagatgcacc gcgtttccct gcgtgtcggc gaaaatctgc acttcgatat ggcgcgggcg
      601 tgtaacgtat ttctcgatca gaacccggtc gtcaccgaac gccgactgcg cctcgcgctt
      661 cgccccttca agcgcgcctt cgaattcatc cggagcgtcg acccggcgca tccccttgcc
      721 gccacccccg gcgaccgcct tgataagaac cggaaagccg attcgcccgg cctcgtgcga
      781 caatttcggc aacgattgct ctgcgccgag atagccaggc accaccgcga cgcccgcctt
      841 cgccatcagt ttcttggcgc ggtccttcaa ccccatcgcg cggatcgcgt cgggaggcgg
      901 gccgacaaag gcaatgcccg cagaggcgca ggcttccgcg aattcggcat tctcggaaag
      961 aaacccatag ccgggatgaa tcgcatcggc tgccgatttc ctggctgccg aaatcaccgc
     1021 gtcgatgttc agatagctct cgcgcggcgc ggccgcgccg atgcgaacgg cttcgtcggc
     1081 cgcctccaca tgcgccgcac gcgcgtccgc gtccgaatag acggcgaccg tcgctatgcc
     1141 gagatgccgc gccgttcgaa cgacccggat tgcgatctcg ccgcgattgg cgatcagcag
     1201 cttgcggaat agcggcgaca cgcggcgtta catccggaaa acgccgaagc ggctgtcggg
     1261 aatgggcgcg ttgaggcagg cggaaagtgc cagggcaaga accctgcgcg tgtcggccgg
     1321 cgcaatcatg ccgtcgtccc acagccgcgc ggtggcataa taggggttac cctcctcctc
     1381 ataacgtgcg cggatcggcg ctttgaacgc atcttcgtcc gcggcactcc agctctcgcc
     1441 gcgggattcc atgccgtcac gcttgacggt cgcgagcacg cttgcggcct gctcgccccc
     1501 catcaccgag atgcgggagt tgggccacga aaagagaaac cgcggcgagt aggcgcgccc
     1561 gcacatgccg taatttcccg cgccgtagga accgccgatc agaaccgtaa tctttggaac
     1621 cgccgcgcac gcgacggcag tgaccatttt cgcgccgtcc ttggcgatgc cgcccgtctc
     1681 gtacttccgg ccgaccatga agccggcgat gttctgcagg aaaagcagcg gcacgcggcg
     1741 ctggcagcac agctcgatga aatgcgcggt tttgagggcg gattcggaaa acagaatgcc
     1801 gttgttcgcc acgattccca ccggcattcc ctcaatatgg gcgaaacccg taacgatggt
     1861 cggcgcgtat agccgtttga actcgtcgaa aaccgaacca tccaccagtc gggcgatcac
     1921 ctcatggacg tcgtactgga tcgacaacga ctgcggaaca attccgtcaa gctccgcgat
     1981 atcgtagagc ggcggcttcg gttcggcgca cgcaatatca ggcgatttcg tggcgttcag
     2041 attcgccacg atgcggcgaa ggatggcaag ggcatggctg tcgtcattgg cgtagtgatc
     2101 ggctactccg gaaacgcgcg cgtgaacctc cgctcctccc agatcttccg cgctcacgat
     2161 ctcgcccgtc gccgccttca cgagcggcgg tccaccaagg aagatggtgc cctggccttt
     2221 gacgattatc gtctcgtcgg acatggccgg cacgtaagct ccgcctgcag tacaggagcc
     2281 catcacggcg gcgatctggg gaatgccgag cgccgataaa ttcgcctgat tgtagaagat
     2341 gcgtccgaaa tggtcgcggt cgggaaagat ctcggcctgg ttgggaagat tcgcgccgcc
     2401 ggagtcgacg agatagacgc agggcagccg gttctcgcgc gctatttcct gcgcgcgcaa
     2461 atgcttcttg accgtaacgg ggtaatacgt cccacccttg atcgtggcgt cgttgcacag
     2521 gatcatgcat tcccggccgt cgatgcgtcc gatgccggtg atgattcccg cagcgtggat
     2581 atcgccgtcg tacataccga aggcggcgag cggcgaaaat tcgaggaacg gcgaccccgg
     2641 atcgacaagc cgctcgaccc gctcccgcgg caggaacttg ccgcggtccg tatggcgctt
     2701 gcgagagcgt tcatcgccgc cctgcgcggc gagattcatt cgcgatttaa gctcggcgac
     2761 aagggcggcc atcgctgcgg cattggcttt gaatgcggcc tcgcgcgttg cgattgccgt
     2821 tttgataacg ctcatagccg ctcgacggca accgcggtcg cctcgccgcc gccgatgcac
     2881 aacgccgcca cgccgcgttt gaggccgcgt ttctccagcg cgttgagaag cgtcaccacg
     2941 atgcgggctc cggacgcccc gatcgggtgg cccagcgcac acgctccgcc gttcacgttg
     3001 actctctcgt gcggcaagcc gaggtcgcgc atcgcaatca tggcgacaca ggcaaaggct
     3061 tcattgattt cgaagagatc gacgtcctcc ttgcgccatc ccgaagcgtc gagaactttg
     3121 ccgatcgcgc cgacgggcgc ggtcgtgaac caccgcggct cggccgcatg actcgcctgc
     3181 gccacgattc gtgcaatcgg cttaagaccg cgcctggctg caaaatcttc ccgcgcaact
     3241 acg
//
LOCUS       DASZMC010000377         4194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3183281,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000377 DASZMC010000000
VERSION     DASZMC010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3183281"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VG274_07720"
     CDS             <1..738
                     /locus_tag="VG274_07720"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HWE06581.1"
                     /translation="RIGPAQRFAMTFDGGQYQELPQPSVPADPLKFRDERRYTDRLKD
                     ARNKTGRAEALSAARGTLDGMLVMIAVQPFDFLGGSLGMGVGEGLVLAMQAAVQERRP
                     FILFVASGGARMQEGILSLMQMPRVTIGVDMLREAGLPYIVVLTDPTTGGVSASYAML
                     GDVQIAEPGALIGFAGQRVIEQTIREKLPEGFQRAEYLLDHGMLDMVVHRRDLRATLS
                     KLVHLLMKQPQPQAKAAAALAALPPAP"
     gene            735..2045
                     /locus_tag="VG274_07725"
     CDS             735..2045
                     /locus_tag="VG274_07725"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HWE06582.1"
                     /translation="MSAGAASDAILQRLLSLHPKRIDLVLDRIQRLLAALDHPERRLP
                     PVLHVAGTNGKGSICAFARAMLEAQGLRVHVYTSPHLVHFQERIRIAGRLIGEEELAA
                     TLEDCERVNARLPITFFEITTAAALLAFSRHRADALVLEVGLGGKYDATNIIDRPRAT
                     AIAPVGLDHQEFLGSTLVDIAAEKAGIIKQGVPVIVGAQEDVPRDVIARRAQALGAPH
                     FQFGQDFFAHQEQGRMVYQDGKGLLDLPMPRLVGRHQIDNAAVAIAMLRAAGWGEERA
                     IEKGLRSVEWPARMQRLSRGPLIELAPRDAEIWLDGGHNPQGAAVIARTIADFEERVE
                     KPLYLICGMLKTKDARGFFAPFHGLARHVATVAIEGDEASRGAGELYDAARASGLDAS
                     PAEDLEDAMMQVTAWSRARPFEGSPRILICGSLYLAGRVLAENG"
     gene            complement(2111..2524)
                     /locus_tag="VG274_07730"
     CDS             complement(2111..2524)
                     /locus_tag="VG274_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWE06583.1"
                     /translation="MAHNSYHIYGLVGIGLGLYSAFMLDPQDWKCARRRNRRLSQGLA
                     MAPAIHPGRLLKRGLKTRNISGNRLALDLGAPSGRIADMLNGRRSISADTAVRLGRYF
                     GNRAQFWLDLQSQYEIAVVEREHGTEIASRVRSAA"
     gene            2671..4107
                     /locus_tag="VG274_07735"
     CDS             2671..4107
                     /locus_tag="VG274_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWE06584.1"
                     /translation="MATEALTTATPSPAIQRVLPWLVAVAFFMESLDTTILNTAVPIV
                     AKAIGTTPLSMKAVLASYTLSLAVFIPVSGWMADRFGTRRVFASAIGIFTLGSLLCGL
                     MSNIHLLVACRVLQGCGGAMMVPVGRLTLVRSFPRSELIRAMSFVAIPALVGPMLGPA
                     AGGFIVAYFHWRAIFFLNVPVGIAGLVLVWMKLPDYRERTKPLDIEGLILFGAGLALL
                     SYVLEVFGEHTLSAPEILGFLCVSLALLFGYGVHARRTRWPLLRLGLFRIRSFAGAVG
                     GGFVTRLGIGGVPFLLPLLYQVGLGFSPVQSGLLIMPQAAAAMSTKIVIRQVLQRFGY
                     RTVLIGNTLLMSVFLMLFATIGTGTPVWLIVLQAFCYGALTSTQYTSMNTLAYADVSP
                     AKASAASSIASTSQQLSISFGVAIAGLATMFFVPPAARSSPIEMIHGLHHAFLALGLF
                     TLLSTVVFSRLKREDGADETRQKDIHLG"
BASE COUNT          674 a         1382 c         1360 g          778 t
ORIGIN      
        1 cgcatcgggc ctgcccagcg tttcgcgatg acgttcgatg gcggccagta ccaggagctg
       61 ccgcagcctt cggttccggc cgatccgctc aagttccgcg acgagcgccg ctacaccgat
      121 cgcctgaagg atgcgcgcaa caagacgggc cgcgccgaag cgctgtcggc cgcgcgcggc
      181 acgctcgacg gcatgcttgt catgatcgcc gtgcagccct tcgattttct cggcggctcg
      241 cttggcatgg gcgtgggcga gggactggtg ctcgcgatgc aggcggcggt gcaggagcgc
      301 cggccgttta tcctcttcgt cgcttcgggc ggcgcacgca tgcaggaggg cattctctcg
      361 ctgatgcaga tgccgcgcgt caccatcggc gtcgacatgc tgcgcgaggc cgggctgccg
      421 tacatcgtcg tgctcaccga tccgacgacc ggcggggtga gcgcgtcata cgcgatgctg
      481 ggcgatgtgc agatcgcaga accgggcgct cttatcggct ttgccggaca gcgcgtcatt
      541 gaacagacga ttcgcgaaaa attgcccgaa ggcttccagc gcgcggaata tctgctcgac
      601 cacggcatgc tcgacatggt ggtgcaccgc cgcgacctgc gcgccacctt gtcgaagctc
      661 gtgcatctcc tgatgaagca gccgcagccg caggcgaaag ccgccgccgc actcgccgcg
      721 cttccgccgg ccccatgagc gcaggcgcgg cgagcgacgc cattctccaa cggctgctct
      781 cgcttcatcc caaacgcatc gaccttgtgc tggaccgcat ccagcgcctg ctcgccgcac
      841 tcgatcatcc ggagcgcagg ctgccgccgg tgctccatgt cgcgggaacg aacggcaagg
      901 gatcgatctg cgccttcgcg cgcgcgatgc tcgaagcgca agggcttcgc gtgcatgtct
      961 acacgtctcc ccatctcgtg catttccaag agcgcatccg catcgccggc cggctgatcg
     1021 gcgaggagga acttgccgcg acactggaag attgcgagcg ggtcaacgcg cgactgccga
     1081 tcaccttctt cgagatcacg acggctgcgg cgctccttgc cttctcgcgc catcgtgccg
     1141 atgcgctggt gctggaggtg gggctgggcg gaaaatacga tgcgacgaat atcatcgacc
     1201 ggccgcgcgc gaccgcgatc gcgccggtcg gcctcgacca ccaggaattc ctcggcagca
     1261 cgctcgtcga catcgccgcc gaaaaggccg gcatcataaa acaaggtgtg ccggttatcg
     1321 tcggcgcgca ggaagacgtt ccgcgcgacg tcatcgcgcg ccgcgcgcag gcgctggggg
     1381 cgccgcactt tcagttcggc caggatttct tcgcccatca ggagcagggc cgcatggtct
     1441 atcaggacgg gaagggcctg ctcgacctgc ccatgccgcg gctcgtcggc cgtcaccaga
     1501 tcgacaatgc ggcggtcgcc atcgcgatgc tgcgggcggc gggctggggc gaggagcgtg
     1561 cgatcgagaa gggattgcgc agtgtcgaat ggccggcgcg catgcagcgg ctgtcgcgcg
     1621 ggccgctgat cgaactggcg cccagggatg cggaaatctg gctcgacggc ggccacaatc
     1681 cgcaaggggc ggccgtcatc gcacgcacga ttgccgattt cgaggagcgc gtggagaagc
     1741 cgctctatct catctgcggc atgctgaaga ccaaggacgc gcggggtttc ttcgccccgt
     1801 ttcacgggct cgcgcgccac gtggcgacgg tggcgatcga gggcgatgaa gcaagccgcg
     1861 gcgcgggcga gctttacgac gcggcgcgcg ccagcggcct cgacgccagt cccgccgaag
     1921 atctcgaaga cgcgatgatg caagtgaccg cctggtcgcg cgcgcgcccc ttcgaaggct
     1981 caccccgcat cctcatttgc ggctcgctct acctggccgg ccgcgtgctg gcggagaacg
     2041 ggtgagggac ggcggattcg aatcctgtcc ggtgcgccag atcgcgccgc agggctgcgg
     2101 ggaacgacgg ttacgccgcg cttcgaacgc ggcttgcaat ctcggttccg tgctcccgct
     2161 cgacgaccgc aatttcgtat tgactctgaa gatcgagcca gaattgcgca cggtttccaa
     2221 aatatcggcc caaccgcacc gccgtgtcgg ccgagatcga gcggcggccg ttgagcatgt
     2281 cggcaatgcg gcccgacggc gcgccgagat cgagtgcgag ccgattcccg ctgatattgc
     2341 gcgtcttaag cccgcgcttc aggagacgtc caggatgtat tgcaggcgcc atcgccagtc
     2401 cctgcgataa tcgacgattt cgacgtcgtg cgcatttcca gtcctgagga tcaagcatga
     2461 aagccgaata aagcccaagc ccaatcccga caaggccgta gatgtgatac gaattgtgtg
     2521 ccatgcggcg tttgcagcaa ggtcaacgga cagcgtgacg gtcaattctt acagaacatt
     2581 tacggcatgc cagcccggcg ttttcgggcc aaagaccagc aacacggtcg ggcgagggcg
     2641 cccgcgatcc cgggagcgtg tgcgaaaagc ttggcaaccg aagccctgac aaccgcgacg
     2701 ccgtctcccg cgatccagcg tgtgctgcct tggctcgtgg cggtggcgtt tttcatggaa
     2761 tcgctcgaca ccacgatctt gaataccgcg gtgcccatcg tcgcgaaggc gatcggcacg
     2821 actccgctca gcatgaaagc cgtgctggcg agctacacgc tcagcctcgc cgtcttcatt
     2881 ccggtcagcg gctggatggc cgaccggttc ggcacgcggc gcgtgttcgc ttcggccatc
     2941 ggcatattca cgttgggctc gcttctgtgc ggactgatga gcaacatcca tctcctcgtc
     3001 gcctgccgcg tgctgcaggg atgcggcggc gccatgatgg tgccggtggg ccggctcacg
     3061 ctggtgcgca gctttccgag atcggagctg atccgggcga tgagcttcgt tgccatcccc
     3121 gcgctggtcg ggccgatgct gggccccgcc gcgggcggat tcatcgtcgc ctattttcac
     3181 tggcgcgcga ttttcttcct caacgtccct gtcggcattg ccggactggt gctcgtctgg
     3241 atgaagctgc cggactatcg cgagcggaca aagccgctcg atatcgaagg ccttatcctc
     3301 ttcggcgccg ggctcgcgct tctgtcctat gtgctcgagg tcttcggcga gcacacgctg
     3361 agcgcgccgg agattctcgg gtttctgtgc gtttcgctgg cgctcctgtt cggctatggc
     3421 gtgcatgcca ggcgcacgcg ctggccgctg ctgcggctgg gcctgtttcg catccgctcg
     3481 ttcgccggcg cagtcggcgg cggcttcgtc acgcggcttg gcatcggcgg cgttccgttc
     3541 ctgctgccgc tcctgtatca ggtgggactg ggattctcgc ccgtgcagtc gggtcttctc
     3601 atcatgccgc aagccgctgc tgcgatgagc acgaagatcg tgatccggca ggtcttgcag
     3661 cgcttcggct atcgcacggt gctgatcggc aacacgctgc tcatgagcgt cttcctgatg
     3721 ctgttcgcca ccatcggaac cggcaccccg gtctggctga tcgtgctgca ggctttctgc
     3781 tacggcgcac tgacttcgac ccaatatacg agcatgaaca cgctcgccta tgccgacgtg
     3841 tcgccggcga aagcgagcgc ggcgagctcc atcgccagca cctcgcagca attgtcgatc
     3901 agcttcggcg tggccatcgc gggccttgcc accatgttct tcgtgccgcc ggccgcgcgg
     3961 tcatcgccga tcgagatgat ccacggtctg caccacgcct tcctcgcact cggccttttc
     4021 accctgcttt caacagtcgt gttcagccgt ctcaagcgcg aagacggcgc cgacgagacg
     4081 cggcagaagg acattcatct ggggtaaatt tggtggttgc acggtcacgt cggcagcgcg
     4141 gacttccggc gggtcacaac cgcgacccgg tccccactta tatcctgccc ggtg
//
LOCUS       DASZMC010000378         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_973323,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000378 DASZMC010000000
VERSION     DASZMC010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973323"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..309)
                     /locus_tag="VG274_07740"
     CDS             complement(103..309)
                     /locus_tag="VG274_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06585.1"
                     /translation="MNAASESIVRDASKLTEEDRSKDEFFVKLAHVAEAMIAAHGKDF
                     AMGALILTARFIAEGKPLTKPEVR"
     gene            complement(306..674)
                     /locus_tag="VG274_07745"
     CDS             complement(306..674)
                     /locus_tag="VG274_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06586.1"
                     /translation="MTGDGDSPSDARRPEREVEASQAGRKSMLARHKLIEAMIENNLR
                     QLKFDSARGGADIERACALRDIERGGCDSEPTERLAEIDRRIEQLEDEHRSLVAEREW
                     LNRSLLEFDDQASANRRFLT"
     gene            complement(671..1900)
                     /locus_tag="VG274_07750"
     CDS             complement(671..1900)
                     /locus_tag="VG274_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWE06587.1"
                     /translation="MSQQSCSSDLFLNGTVPDQPASNTLIDISRLSPWHGACAQLRNC
                     AQLAADRYHAQRFTSGRNMKASVLHAYDETLTAKEFVSYEDVADPKISQPSDVIVRIG
                     GAGVCRTDLHIVEGIWRRKVEVNLPYIMGHENAGWVEEIGKGVQGVKVGDAVICHPLV
                     TSGHCLACRRGDDMHAEDSSFPGINANGGYAQFLLTGERTLIKLPKTLAPKDVAPYTD
                     AGLTAYRAAKKASRHLLPGEYAVVVGAGGLGHIGIQVLSALCAAEIIVVDRAEKPLAL
                     AKDCGADHVVKADGGEVDAVLKLTKGRGAEAVIDFVGEGDAVAKGLAMTRNGGSYYVV
                     GYGGKIEIPTIDMITSEKTIVGNLVGTYAELVELMALADRGLVKLHTREYELSAANDA
                     LHDLHDGRVNGRAVLIP"
     gene            2492..>3486
                     /locus_tag="VG274_07755"
     CDS             2492..>3486
                     /locus_tag="VG274_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWE06588.1"
                     /translation="MLDIVADKSFADTPEARNIRPGSLWSEALCGTNGIGTAAFLKRP
                     VVVHGSEHYFPSFGSLTCAASPIFAPDGSLAGILDASSDCVSRQTHTAALVCMAATQI
                     ENGLFRERHHRDILIAFHNRGEYLHTLTAGLIAVDDSGRILAANQAARILLHGLPTTP
                     GRSFTDIFRTRFTSFMDEGRLRERQRLQDEVGSSFIATIEHPNVSPMLRAVARPRVAA
                     SAVTPTPFISVDPVVARMVRQTEAAAQRKMPILIRGETGTGKEQLARHAHAASGRSGS
                     FVAVNCAALPESLIEAELFGYSEGAFTGARKGGSAGLVKEGDGGTLFLDEIGDMPV"
BASE COUNT          612 a         1074 c         1037 g          763 t
ORIGIN      
        1 ggatcgctcg tcgatcctgc atcgactttc acccgtcgac ccgccgttct ctccggatag
       61 ttgcggcgga ttcgtgctaa gtcgcggcgt tcattcagtc gctcatcgta cttccggttt
      121 ggtgagcggc ttgccttcag cgatgaagcg ggcggtcaga atcagcgcgc ccatcgcaaa
      181 gtctttgccg tgcgcagcga tcatcgcttc ggcgacatgc gccagcttca cgaaaaactc
      241 gtccttcgag cgatcttcct ctgtcagctt gctagcgtcg cgaactatcg actcgctggc
      301 ggcgttcatg tgagaaacct ccgatttgcg gacgcctgat cgtcaaattc gagcagggac
      361 ctattgagcc attcgcgttc agcgacaaga cttcggtgct cgtcctcgag ctgctctatg
      421 cggcgatcga tttcggcgag acgctcggtc ggctcagaat cgcagccccc gcgctcaatg
      481 tcgcgcaaag cgcacgcgcg ctcgatgtcc gcaccgcccc gcgcgctgtc aaacttgagt
      541 tgccgcaaat tgttctcgat catcgcttcg atcagtttgt gtctggccaa catgctcttg
      601 cgacccgcct gcgaagcttc gacctcccgt tcgggccgtc gggcatcgct ggggctatcg
      661 ccatcgcccg tcatgggatc aggaccgcgc gcccgttgac gcggccgtcg tgcaggtcgt
      721 gcaaagcgtc gttagctgcg ctcaactcgt attcgcgcgt atgcagctta acgagcccgc
      781 gatccgccaa cgccatgagt tcgaccagct cggcataggt gccgactaga ttgcccacga
      841 tcgtcttctc agatgtgatc atatcgatcg tcggaatctc gatctttccg ccgtagccga
      901 cgacgtaata gcttccgccg tttcgagtca tcgccaagcc tttggcgact gcgtcgccct
      961 cgccgacgaa atcgatcacc gcctcggcgc ccctgccctt tgtcaacttc aggaccgcgt
     1021 cgacctcgcc gccgtcggcc ttcaccacgt ggtccgcgcc gcagtccttg gcgagcgcca
     1081 ggggcttttc agcgcggtcg acgacgatga tttcggcggc gcaaagcgcg ctcaacacct
     1141 ggattccgat gtggccgagg ccgccggcgc cgacgacgac ggcgtattcg cccggcagaa
     1201 ggtgccgtga cgctttcttg gccgctcgat aggcggtgag gcccgcatcc gtgtaggggg
     1261 cgacgtcttt aggcgcgagt gtcttcggca gtttgatcag cgtgcgctcg cccgtcagca
     1321 aaaactgcgc gtagccgccg ttggcgttga tgccgggaaa gctgctgtcc tccgcatgca
     1381 tgtcgtcgcc gcgacgacac gccaagcaat ggccgctggt gaccagcgga tggcagatga
     1441 ccgcgtcgcc gactttcact ccctgcacgc ctttgccgat ttcctcgacc cagccagcgt
     1501 tttcgtgacc cataatgtaa ggaagattga cctcgacctt gcggcgccaa atgccttcga
     1561 cgatgtgcaa gtcagttcgg cacacgccgg cgcccccgat gcgcacaatg acatccgatg
     1621 gctggctaat cttggggtcc gccacgtctt cgtagctgac gaattccttg gccgtcagcg
     1681 tctcgtcgta cgcgtggagg acggaggcct tcatgtttct tcccgatgta aaacgctgag
     1741 catgatatct atcagctgcc aactgcgcgc agttgcgcaa ttgcgcgcaa gcaccgtgcc
     1801 acggtgataa acgactgata tcaatcagag tgttgctggc gggctgatcc ggcactgttc
     1861 cgttgaggaa cagatcgctg gaacagcttt gttgcgacac gaaacaggct cgtgtccgta
     1921 ttcgcactgg ggaatcaacg ggcgtttttc ggcagggcgc agatcggctc tagccactaa
     1981 ccgagcgcat aacctaaccg caattttgcg ttaagcttgc gcgcgtcggc tgcgcacatc
     2041 gggaggtggg actgcgacgc gaacgcgcat tgtggaaggg ttgctttcgg tttctggttc
     2101 gttattcttc ggcgttgaac ttgcgcaaag aaaagccgtt cggggagatt cgcgtgcgag
     2161 gcgccgtagg gccctcgcga cctgtggagg tctaatgcaa ttatccgtcg cagagcgggc
     2221 gacccaggcg catggcatcc tgcggcagtc cggcacggtc ccgagcaatc ttctcgccgc
     2281 tgaaatctat gagagctggt tgcgttgcgt tgcgcttggc ctcgattcga caaggccgcc
     2341 gtcgatcgac ttggcgagcc ctaaggtcct gcgttccgag caggagcaac gtgtcctgct
     2401 tcgcggtctt gcgctcgcgg aaatgcgaac cttgcacgat caaattgcgg gttcgaattt
     2461 cctcatcgcc tttgcgacgc ccgagggtat gttgctcgat atcgttgccg acaagagttt
     2521 cgctgacact cccgaagcca ggaatatccg ccctggcagc ctatggtcgg aagcgctttg
     2581 cggcacaaat gggatcggta cggccgcgtt cctcaagcgt ccggtcgtgg tccacggcag
     2641 cgagcattat ttcccgagtt tcggttcatt aacttgcgct gcttctccga tttttgcgcc
     2701 ggatggttca ctagcgggca tccttgatgc ttcgtccgat tgcgtctccc gtcaaaccca
     2761 taccgctgcc ctcgtttgca tggcggcaac gcagatcgag aacgggctct ttcgcgagcg
     2821 ccaccatcgc gacatcttga tcgcctttca caatcgcggc gagtacctgc atacacttac
     2881 agcgggcctc atcgcagtcg acgattcagg ccgcatactg gctgctaatc aggcggcgcg
     2941 cattttgttg catggtttgc cgaccacgcc aggccgcagc ttcactgata tattcaggac
     3001 gagatttacc tccttcatgg atgaggggcg gttgcgtgaa cgccaacgcc tgcaggatga
     3061 ggtcggcagc agtttcatcg ccacgatcga acatccgaac gtgtccccga tgttacgcgc
     3121 cgtcgcaaga ccgcgggtcg cggcttctgc cgttacgcct acgccattca tctcggtcga
     3181 ccccgtcgtt gctcgcatgg ttcggcagac agaggctgcg gcgcagcgca agatgccgat
     3241 tctgattaga ggggaaacgg gcacggggaa ggagcaactg gcgcgtcacg cccatgctgc
     3301 gagcgggcgc agcggctctt ttgtggctgt caattgcgcc gccttgcccg agagcctgat
     3361 cgaggccgaa ctgtttggtt atagtgaagg cgcatttacc ggcgcccgca aaggcggatc
     3421 tgccgggctg gtcaaggagg gggacggcgg gactctgttc ctcgacgaga tcggcgacat
     3481 gccggt
//
LOCUS       DASZMC010000379         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5104975,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000379 DASZMC010000000
VERSION     DASZMC010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5104975"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VG274_07760"
     CDS             complement(<1..949)
                     /locus_tag="VG274_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HWE06589.1"
                     /translation="MTDISHQIWDMKYRFKAADGTPVDRDFADSWARVAMAAAAAEAP
                     EKRRQWAIEFARALAGQRFLPAGRILAGAGTARSVTLFNCFVMGTIPDDMGGIFAHLR
                     EAALTMQQGGGIGYDFSTLRPQGAPVAGVGADASGPVSFMGVWDAMCRTIMSAGSRRG
                     AMMATLACDHPDIETFIDAKRTPGQLSMFNLSVLASDAFIDAVRNDSEWELKFGGKVY
                     RSLQARALWNRIMRATYYYAEPGVIFIDRINEKNNLAYCETIRATNPCGEQPLPPYGA
                     CLLGSINLAKLVRSPFADDAHLDESELARLTAVAVRLLDN"
     gene            complement(1215..1961)
                     /locus_tag="VG274_07765"
     CDS             complement(1215..1961)
                     /locus_tag="VG274_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWE06590.1"
                     /translation="MIWLRSIVFFVWFLLISIVLHVVFLPALVLPRRGAVIAARSWCA
                     GILWGLKVFAALDYEVRGEIPDMPVLVASKHMSMWDTVALFFLLGDPVFVLKRELMRV
                     PFYGWYARKVGMIAIDRQGGGIALRAMTRAAREATARGLGIVVFPEGTRKSVGAPPDY
                     KSGIVALYQGLDIACVPVALNSGLFWTGFLKKPGRIIIRFLAPIRPGLKRKEFIAALE
                     ERIEQTTADLLSEGRALLTRANSRSGKQRF"
     gene            complement(1958..2623)
                     /locus_tag="VG274_07770"
     CDS             complement(1958..2623)
                     /locus_tag="VG274_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWE06591.1"
                     /translation="MKVSHASVLERAFWVAAMVLIAAYFAGFAAFVTDLPASAPNTVR
                     ADAVVTLTGGEDRLDKAVALFERGVGQRLLITGVHPFMTKDRLRRLVHGGRRFDCCAD
                     LGFSATNTHGNAVEAAAWVRAHGYRSLVVVTANYHMPRSLAEFSAAMPGVRLVPYPVP
                     EIMSDRNWWLDPGAMRTLQFEYAKYLGAVALTSIVRAAHGNQDPAQGGGTGGAAVSVS
                     GPS"
     gene            complement(2724..>3091)
                     /locus_tag="VG274_07775"
     CDS             complement(2724..>3091)
                     /locus_tag="VG274_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06592.1"
                     /translation="IAVATAAAVTFATRAGLAAHHDIVSLLHQMGARASFVARSFEWH
                     YCVSALAASALGAALAGVLFALAGSLEFAGLEPVPFLPPLSLGPGELAWLLAVPAGSG
                     IIALVTARISILAALGRNY"
BASE COUNT          632 a          995 c          974 g          490 t
ORIGIN      
        1 cgttgtcgag gaggcgcaca gcgacggccg tgagccgcgc aagctcactc tcatcaaggt
       61 gcgcgtcgtc cgcgaacggg ctgcgaacaa gcttggcgag attgatcgag ccgagcagac
      121 aggcgccgta gggaggcaac ggctgttcgc cacacggatt ggtcgcgcgg atcgtctcgc
      181 aataggcgag attgttcttt tcgttgatgc ggtcgatgaa gatcacgccg ggttcggcat
      241 agtagtaggt cgcgcgcatg atacggttcc acagcgcgcg cgcctgcagg ctgcgataga
      301 ctttgccgcc gaatttcagc tcccattccg aatcgttccg gacggcgtca atgaaggcgt
      361 cgctcgccag caccgaaaga ttgaacatcg agagctggcc cggcgtccgc ttcgcgtcga
      421 tgaaagtctc gatgtcggga tggtcgcagg cgagcgtcgc catcatcgcg ccgcggcgcg
      481 atccggcgga catgatcgtg cggcacatcg catcccacac gcccatgaac gacaccggcc
      541 ccgacgcatc ggcgccgacg cccgcgacgg gcgcgccctg cggccgcaag gtggagaagt
      601 cgtaaccgat gccgccgccc tgctgcatgg tgagcgcggc ctcgcgcaga tgggcgaaga
      661 tgccgcccat gtcgtcgggg atcgtgccca tgacgaagca gttgaacaaa gtgacgctgc
      721 gcgcggtgcc ggcgcccgca agaatacggc cggccggaag gaatcgctgg cccgccagcg
      781 cgcgggcgaa ttcgatcgcc cattgccgcc gcttttccgg cgcttcggcc gccgcagccg
      841 ccatcgccac acgcgcccag ctgtcagcaa agtcgcgatc gaccggtgtg ccgtcggccg
      901 ccttgaagcg atacttcatg tcccagatct gatgggaaat atcggtcatg cgcctacctt
      961 gaccgccacg ggcccaagct ccgccggcgg gccagaaagc taataacatc aaccgttaaa
     1021 gtcaagaatt gttccccata cgttctccta atcgctcgaa acacggagaa tgccaaatac
     1081 cgacgctaat gcattgcctc aagaatttta acacagaggc cgtggggagc gcggagggca
     1141 cggggcaagg catagcgcgc ggtgcgcgca aagaatttcc ccacgctctc cgcgcaacct
     1201 ctgcgacctc cgcgttaaaa tctttgcttg cctgaacggg aattcgcgcg cgtcagcagc
     1261 gcgcggcctt cgctgagtag atccgctgtc gtttgctcga tgcgttcttc gagagccgca
     1321 atgaattcct tccgctttaa cccgggacgt atgggcgcga gaaaccgaat gataattctc
     1381 cccggcttct tcagaaaacc cgtccagaac aatccggaat tgagcgccac cggtacgcag
     1441 gcaatgtcga gcccctggta gagggcgacg atgccggact tgtagtccgg cggagcgccg
     1501 acggattttc gcgtgccttc cggaaagacg acgatcccga gcccccgcgc ggtcgcctca
     1561 cgtgcggctc tcgtcattgc gcgcaaggcg atgccgccgc cctgccggtc gatggcgatc
     1621 atgccgacct tgcgcgcata ccagccgtag aacggaacgc gcatcagctc gcgcttgagc
     1681 acaaagaccg ggtcccccag aagaaagaaa agggcgaccg tgtcccacat cgacatgtgc
     1741 ttggaagcga cgagcacggg catgtcggga atctcgccgc gaacttcgta atcgagcgcg
     1801 gcaaacactt tcaagcccca caatatgccc gcgcaccagc tccgcgccgc gatgacggcg
     1861 ccgcgtctgg gcagaacgag ggcgggcagg aacacgacgt gcaggacgat gctgatgagg
     1921 agaaaccaga cgaaaaagac gatggagcgc agccagatca tgaggggccg gagacggaaa
     1981 ccgcggcgcc gcctgttccg cccccttgcg ccggatcttg atttccgtgc gccgcacgaa
     2041 cgatgctggt gagggcgacc gcgcccagat atttggcgta ttcgaattgc agcgtgcgca
     2101 tcgcaccggg atcgagccac cagttccgat ccgacataat ttcggggaca ggatatggca
     2161 cgagcctgac gccgggcatg gcggccgaga attccgcgag gctcctcggc atgtgatagt
     2221 tcgccgtgac gacgacgaga ctgcggtatc cgtgcgcacg cacccaggcg gcggcttcga
     2281 cggcgttgcc gtgtgtgttg gttgccgaga aaccgagatc ggcgcagcaa tcgaagcggc
     2341 gcccgccatg caccaagcgc ctcagccggt ccttcgtcat gaacgggtgg acgccggtga
     2401 tcagcaatcg ctggcccacg ccgcgttcga acagagccac ggccttgtcg agcctgtctt
     2461 caccgccggt caacgtcaca acggcgtcgg cgcgcaccgt gttcggcgcg gacgcgggga
     2521 gatccgtgac gaaagccgca aagcccgcga aataggccgc gatcagaacc atcgccgcga
     2581 cccagaatgc gcgttcgagg accgatgcgt gcgagacttt cacaatcctc cctcaatgat
     2641 tggcatgcac acctgcagcc cgccctcatg attctgcaag ttcaccatga gggtcttttc
     2701 ctgacttgtc gaaggacgcc acttcaatag ttccgcccga gcgccgcaag aatggaaatc
     2761 cttgccgtga cgagcgcaat gatacccgaa ccggccggca ctgcgagcag ccacgccagc
     2821 tcgcccgggc cgaggctcaa aggcggcaga aagggcacgg gctcgagccc cgcgaattca
     2881 aggcttcccg ccagggcaaa cagaacgccg gccagggcag ccccgagcgc agacgccgcc
     2941 agcgcggaca cgcaatagtg ccattcgaac gagcgtgcga cgaagctcgc ccgcgcgccc
     3001 atctggtgaa gcagggacac gatgtcgtga tgggcggcga gcccggcgcg cgtggcgaaa
     3061 gtgactgccg cggccgtggc gacggcgatc a
//
LOCUS       DASZMC010000380         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3464187,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000380 DASZMC010000000
VERSION     DASZMC010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3464187"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VG274_07780"
     CDS             complement(<1..443)
                     /locus_tag="VG274_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06593.1"
                     /translation="MTNTASKNAILQARIAVALLFGLVVLGVAWYGVSDESLGRLWSD
                     ILDRPGGPMTFRFILQPAMAIIAALRDGAHDARLGRRPYFWALLHGVRDPQGRSGRLW
                     EGIISTARIIILGVVMDTIYQWVVFKTFYPVQAGVIAILLAFVPY"
     gene            637..1893
                     /locus_tag="VG274_07785"
     CDS             637..1893
                     /locus_tag="VG274_07785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWE06594.1"
                     /translation="MSTAGEDARTSADRAKAVRAFYESHPYPAPIDSLEGRLDRYRSP
                     GRRRAQSLLLWPLEKPRADRTILVAGCGTSQAARYALMEPEARVTAIDISEASLLHTR
                     NLQHKHDIRNLQLHRLAIEQIGELGETFDQIVCTGVLHHLSDPDAGLKSLRDVLTPDG
                     AMHIMVYAPYGRAGIYMMQDYCRLLGIGVRDEDLRDLGETVQALPIDHPIAGVVKRAK
                     DFARPNALVDALLNPQDRAYSVPQIYEWLERCGLKFGRWHEQAPYLPQCGAIDGAPHA
                     ARLAALPPRAQYAAIELLRGTMDKHSFVAYRDDREREAQPITFDGDGWRSFVPVRLPW
                     TLTVKDRAPRGVSAVLINPSHTYPDLALFIDAAQERVLRAIDGERSVAEILRGAGETI
                     GNENGRQFFKRLWEHDLIVFDAKRPP"
     gene            complement(1932..2504)
                     /locus_tag="VG274_07790"
     CDS             complement(1932..2504)
                     /locus_tag="VG274_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWE06595.1"
                     /translation="MDDHPVVHVAYRDAEAYARWAGKELPSEAEWEFAARGGLDGAEY
                     SWGDEFTLGAKPMANTWQGAFPHQNLAEDRFERTSPVGSFPPNAYGIYDMIGNVWEWT
                     TDFWSTRHPADAPKACCVPQNPRGGPEAQSYDPCQPEIRIPRKVLKGGSHLCAPNYCQ
                     RYRPAARHAEPIDTSTSHVGFRCVVRVGRA"
     gene            2618..>2818
                     /locus_tag="VG274_07795"
     CDS             2618..>2818
                     /locus_tag="VG274_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06596.1"
                     /translation="MPGVGHLLAARRELVTPSVFRAIKPAARGKLPLSLRRQFLARPA
                     RVSLGVAIGDMHDGVVVHSADRA"
BASE COUNT          533 a          924 c          879 g          482 t
ORIGIN      
        1 aaatagggga cgaatgcgag caaaatggca atgacccccg cctgaacggg gtagaatgtc
       61 ttgaacacga cccattgata gatcgtgtcc atgacgacgc caagaatgat gatcctcgcg
      121 gtggagatga ttccttccca cagacgtccg ctgcgcccct gggggtcgcg gacgccatgt
      181 agcaaggccc agaaatacgg tctgcgccca agtcgcgcat catgggcgcc gtcacgcaga
      241 gcggcgataa tcgccatcgc cggctgcaaa ataaaacgga aagtcatagg cccgccgggc
      301 cggtcaagaa tgtcactcca gagacggccg agactctcgt cggagacccc ataccaagcg
      361 acaccaagga cgacgaggcc gaagaggagg gccacggcaa tgcgcgcctg cagaatcgca
      421 ttcttcgacg cagtgttggt caaaccgctg ccttctgcga acaagcgata tagcgcgagg
      481 ccctcagcac ctttgcaact tggatcgcgt ccgtgttgac caacatcgag cggaattgtt
      541 ttggatagtt gctgtcctag cgcgtgtcaa agccgctcct tttgcgcggc ggttctgcct
      601 cgccaatagc tctcggaagc tcaaggagcg cagcggatgt caacagcggg tgaagacgcg
      661 cgaacgtccg ccgatcgcgc caaggcggtt cgcgcgttct acgagtcgca cccctatcca
      721 gcgccgatcg acagtctcga ggggcgcctc gatcgctacc ggagcccggg tcggcgccgg
      781 gcgcaatctc tgctgctgtg gcccttggag aagccgcgcg cagatcgaac gatccttgtc
      841 gctgggtgcg gaacgtcgca ggccgcccgg tacgcgctca tggagcccga agcgcgggtg
      901 accgcaatcg acatcagcga agcgagcttg ctccacaccc gcaatcttca acacaagcac
      961 gacatacgaa atttgcagct ccaccggctt gcgatcgagc agatcgggga actcggcgag
     1021 acgttcgacc agatcgtttg cacaggggtg cttcaccact tgagtgaccc ggacgcaggc
     1081 ttaaagtcgc tgcgcgatgt cctcacgccc gatggcgcga tgcacatcat ggtctacgcg
     1141 ccttacggac gcgctggcat ttacatgatg caggactatt gccggctgtt ggggatcggc
     1201 gtccgggacg aagacctgcg cgatctcggc gagacggttc aagcactgcc aatcgaccat
     1261 ccgatcgctg gcgtcgtgaa gcgggcgaag gactttgcac gacccaatgc gctcgtcgac
     1321 gcgttgctga acccccagga tcgcgcctat tccgtgccgc agatttacga gtggctcgag
     1381 cggtgcggcc tcaagttcgg gcgctggcac gagcaggcgc cctaccttcc gcagtgcgga
     1441 gcgatcgatg gcgcgccgca cgcagcgcgg ctcgccgcgc ttccgccgcg cgcgcaatac
     1501 gcagcgatcg agcttttgcg cggaacgatg gacaagcaca gcttcgtcgc ctaccgcgac
     1561 gatcgcgaac gcgaagccca gccgatcaca ttcgacggcg acggctggcg cagtttcgtc
     1621 ccggtacggc tcccctggac gctaaccgtc aaggatcgcg cgccccgcgg cgtctccgcc
     1681 gtgctcatca atccgagtca cacatatcct gatcttgcgc tcttcatcga cgccgctcag
     1741 gagcgggtcc tcagggcgat cgacggcgaa cgttccgttg ccgagattct gcgtggcgcc
     1801 ggcgaaacga tcggcaacga gaacggccgc cagtttttca agcggctctg ggaacacgac
     1861 ctcatcgtgt tcgacgcgaa gcggccgccc tgaggcccgc ggcaggggat gcgcccggct
     1921 ggctgcgccg gtcaggcacg cccaacccgc accacgcagc ggaagccgac gtggctcgtg
     1981 gatgtgtcga tcggctcggc gtggcgcgcg gcaggccggt aacgttggca ataattcggg
     2041 gcgcagaggt gcgagccgcc tttcagcacc ttgcggggaa tgcgaatctc gggctggcag
     2101 gggtcgtagc tctgcgcctc cgggccgccg cgcgggttct gcggcacgca gcacgccttg
     2161 ggcgcgtcgg ccgggtgacg ggtcgaccag aagtccgtgg tccactccca cacattaccg
     2221 atcatgtcgt agatcccgta ggcgttcggc ggaaatgacc ccactgggga ggtgcgctca
     2281 aagcggtctt ccgccaggtt ctgatgcgga aacgcgccct gccaggtgtt ggccatcggc
     2341 ttagccccaa gggtgaactc gtcgccccag gaatattccg cgccatcaag cccgccgcgc
     2401 gccgcaaact cccactccgc ctcgctcggc aattccttgc cggcccagcg cgcataagcc
     2461 tcggcgtcgc gataggcgac atggacgacg ggatgatcgt ccattcccac acattgccga
     2521 tcatgtcata gattccgtag ccgtttggcg gaaacgaccg gacgggcgac gtacgctcaa
     2581 agccgtcctg cttcaggttc tgctgcggaa acgcgccttg ccaggtgttg gccatctgct
     2641 tgccgccagg cgtgaactcg tcaccccaag cgtattccgc gccatcaagc ccgccgcgcg
     2701 cggcaaactc ccactcagcc tccgtcggca gttccttgcc cgcccagcgc gcgtaagcct
     2761 cggcgtcgcg ataggcgaca tgcacgacgg ggtggtcgtc cattccgctg atcgagct
//
LOCUS       DASZMC010000381         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4554749,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000381 DASZMC010000000
VERSION     DASZMC010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4554749"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VG274_07800"
     CDS             <1..1076
                     /locus_tag="VG274_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWE06597.1"
                     /translation="ARRDFTINAMAYDPLTGDVLDFFGGQADVRDRVLRATSEAFSED
                     PLRVLRGLQFACRFDLSLDETTAQMSRAIAPEYGTLAKERVAEEFIKWASQSVRPGRI
                     AEYLSATGWLRHFPEIAALGGVPQDPEWHPEGDVGIHTMHVLNAAARIAIRDALRPED
                     RAVLLFAALGHDFGKPATTELRPRDGRKRWTSWGHEPLGGPITKHFLNRIGIKSAIVE
                     QVVPLVENHLAHRSIASTDGDKRVTPRAVRRLAMRLAPASIEQLIRLIEADASGRPPK
                     DAGLPAEAVRLLAAARAEAVHQKPQPPLILGRHVLPYFDNRPGVHIGEVTHAAYEAQA
                     DGKFSSEAEALQWLEQHLARRPK"
     gene            complement(1087..>1538)
                     /locus_tag="VG274_07805"
     CDS             complement(1087..>1538)
                     /locus_tag="VG274_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWE06598.1"
                     /translation="KSITLGGIASEDAPIVLKNVRLAEGGAMNMLGKLTTDGKIVTHG
                     ILFDTGNATLKPQSMGVLNELAKTLKENAALKIEIDGHTDTDGNAQLNMKLSQDRAEA
                     VKRQLVSMGIDGARLTTKGLGSTKPIDNNTTPEGKANNRRVELVKVG"
BASE COUNT          270 a          460 c          497 g          311 t
ORIGIN      
        1 cggcgcggcg cgacttcacg atcaacgcga tggcctatga tccgctaacg ggcgacgtgc
       61 tcgatttctt tggagggcag gcggatgtgc gggaccgcgt gttgcgcgcc accagcgagg
      121 cgttttcaga agatcctctg cgcgtgctgc gagggttgca gttcgcctgt cggttcgatc
      181 tatcgctgga cgaaacgacc gcgcaaatgt cgcgcgccat tgcgccggaa tacggcacgc
      241 tggcgaagga acgcgtggcg gaggagttca taaagtgggc gtcgcagagc gttcgtccag
      301 gccgcattgc cgaatatctc agcgcgacag gctggctgcg gcactttccg gaaatcgcag
      361 cgctgggcgg cgtgccgcaa gatccggagt ggcatcccga gggcgatgtc ggaatccaca
      421 cgatgcacgt cttgaatgcg gcagcacgga ttgcaattcg cgatgcgctg cgaccggaag
      481 atcgcgcggt gctgctgttc gcggcgctgg gtcacgactt cggcaagccc gccaccacgg
      541 agctacgccc tcgcgacggg cgcaagcgct ggacgtcatg gggacatgaa cctctcggcg
      601 gccccatcac caaacacttt ctaaatcgca tcgggatcaa gagcgcgatt gtcgaacagg
      661 tcgtgccgct ggtggagaat cacctggcgc atcgcagcat cgcgagcacc gacggagaca
      721 aacgcgtcac gccgcgcgcg gttcgccgcc tggcgatgcg tcttgctccg gcgagcatcg
      781 aacaattgat tcgcttgatt gaggccgatg cgtctggccg tccgccgaaa gatgcgggtc
      841 tgcctgccga agctgtacgt cttttggcgg ccgcacgcgc agaagccgtg catcaaaagc
      901 cgcagccgcc gttgatcctg gggcgccacg tgctgccgta tttcgataat cgcccggggg
      961 tgcacatcgg ggaggtgacg cacgcggctt acgaagcgca ggccgatggc aaattctcca
     1021 gcgaagcgga agcgctccag tggctggagc agcatctggc acgccgcccg aagtgatgcg
     1081 ggcgccttag ccgaccttca ccaactccac gcgccggttg ttggctttgc cctcgggagt
     1141 tgtgttgtta tcgatcggct tagtggaacc caagcctttt gtcgtgagcc gggcgccgtc
     1201 gattcccatg gacacaagct ggcgctttac ggcctcggcg cgatcctgcg agagcttcat
     1261 gttgagttgt gcgttgccgt cggtgtcggt gtgcccgtcg atttcgatct ttagtgcggc
     1321 gttctccttc agcgtttttg cgagctcatt gagcacgccc atcgactgcg gcttcagcgt
     1381 ggcattacct gtatcgaaca aaatgccgtg cgtgacgatc ttgccatcgg tcgtcagctt
     1441 accgagcatg ttcatggcgc cgccctcggc caatcttacg tttttgagga cgataggagc
     1501 gtcctcgcta gcgatgccgc ccagggttat cgatttca
//
LOCUS       DASZMC010000382         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4555017,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000382 DASZMC010000000
VERSION     DASZMC010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4555017"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VG274_07810"
     CDS             complement(<1..234)
                     /locus_tag="VG274_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWE06599.1"
                     /translation="MNAAQLNEIAAGYAKAWNSGSPAEVTTLFAADATMIINRGHVLQ
                     GRAAIAGMVTGFYEEFPDLSVQCDGIRSAGSHAV"
     gene            561..881
                     /locus_tag="VG274_07815"
     CDS             561..881
                     /locus_tag="VG274_07815"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWE06600.1"
                     /translation="MSMDAVFRALADRSRRKLLDRLRKRNGQALGELCEGLAMSRQAV
                     TKHLAILEAANLVVSRKEGRTKLHFINAVPINDIAERWIGKFEHRRLSALSALKDILE
                     RNSR"
     gene            878..1327
                     /locus_tag="VG274_07820"
     CDS             878..1327
                     /locus_tag="VG274_07820"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWE06601.1"
                     /translation="MSSDTFIYVTYIRTTPAKLWEALTEPEFNRQYWFGCHQESDWKP
                     GSSWTLKMPDGRIADAGEVLECDPPRRIVLKWRNEFRPELKAEGFTRCTFEIEEADGM
                     AKLTVTHQAERPSVFIDKVSTGWPKILSSLKSLLETGSGLPRTDQPK"
     gene            complement(1362..1700)
                     /locus_tag="VG274_07825"
     CDS             complement(1362..1700)
                     /locus_tag="VG274_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06602.1"
                     /translation="MRNAAAILVVACISTSGCGPQEGGGGHHGGHGLFGMRHGGLPLL
                     HHAMRLGMRGTHAHGFRRACADDVARLCPNAKSHRDERECLEGKRDSLGADCKTALDA
                     RRNTGAENTR"
     gene            complement(1940..>3133)
                     /locus_tag="VG274_07830"
     CDS             complement(1940..>3133)
                     /locus_tag="VG274_07830"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWE06603.1"
                     /translation="RLHGASLFSAKIHATGATAGTGKSFGTKCEAAGARLVLHGAAKL
                     TFMTGIAGAILFAWQGIALHVQQFSVRTDPMAPVAAVAKWIHTTWNAVVPQLPEIPES
                     TFAAENRMSASQLVNRWTPLIASASQRFSVPANWIRAVVQMESGGRTMLNEDTPITSR
                     TGAMGLMQLEPGTYQEMRTQYRLGPNAYNPGDNIAAGAAYLRWLYSKYGYPAMFAAYN
                     DGPGHLEERLMKGKLLPDETRNYISRIVTALGSAGAAVVGSASAAQAELVKFTRPNGT
                     PVWIDVAGATLARASLPGEYAPGVKSVITVGRLKQGVVEDLASVQAAIRAHGGIVEPR
                     ATRLAGAHGVHTLRMAVTGKSRVAHRYAALAHRGRVHVTFREAAANSGAIHLKPIAHG
                     GRAKG"
BASE COUNT          564 a          982 c          997 g          590 t
ORIGIN      
        1 caccgcgtgg ctgcccgcgc tgcgaatgcc atcgcattgc acggagaggt cggggaattc
       61 ctcatagaag ccggtcacca tgccggcgat tgccgcccga ccctgaagga catgaccgcg
      121 gtttatgatc atcgtggcgt cggctgcgaa cagggtggtg acctccgccg gcgagcctga
      181 attccacgcc tttgcgtagc ccgccgcgat ctcgttcagt tgtgccgcat tcattgcatt
      241 gtctccacac catccgctga taccgagctc cgatgccagc ctatcggtcg tcggactggt
      301 ttgttctcta gcgaaacttc actagcatcg gagccgcgcg gatttttgtc cagcaccatc
      361 ctccaacgta ggggctctgg cgttctgcat attgggtgtt ccggtgataa ctgtctgcgt
      421 gctcgcggcg gtcagtcctg gggcactctc ctcactggcg ggaatgtcag gctctacagc
      481 gccgcctccg gcggcggcgc cagagacgat ggcacggtgt tcaggcttga catgcaacca
      541 aatggttgca tattagcgtc atgagcatgg atgcggtgtt ccgggcgctg gcggacagga
      601 gccgccggaa gctgctggac cgtctccgta agcgaaacgg acaggcgctc ggcgaattgt
      661 gcgagggatt ggcgatgtcg cgtcaggcgg tgaccaagca tctggcgatt ctcgaagcgg
      721 cgaaccttgt cgtgtcgagg aaagagggcc gcacgaaact gcacttcatc aatgccgtgc
      781 ccatcaacga catcgccgaa cgctggatcg gcaaattcga gcatcggcgc ctcagcgcgc
      841 tttctgccct caaggacatt ctcgaaagga actcacgatg agcagcgata ccttcatcta
      901 cgtgacctat atccgcacga cgcccgcaaa attgtgggag gcactgacgg aacccgaatt
      961 caaccgccaa tactggttcg gctgccacca ggaatcggac tggaagcccg gctcgtcctg
     1021 gacgttgaag atgcccgacg ggcgcatcgc cgatgcgggc gaagtgctcg aatgcgatcc
     1081 gccgaggcgc atcgtgctca aatggcgcaa cgaattccgc cccgagctga aggcggaagg
     1141 attcacgcgc tgcaccttcg agatcgagga ggcggacggc atggcgaagc tcaccgtcac
     1201 gcaccaggcg gaacgtccga gcgtcttcat cgataaagtg tcgaccggct ggccgaaaat
     1261 cctctcaagc ctgaagagcc tgctcgaaac cggcagcggc ctgccgcgta ccgaccaacc
     1321 gaagtgacgc ccacactccg ttaaggggag ggtggaaaga atcagcgcgt gttttctgcg
     1381 cctgtgttcc gtctcgcatc gagagcagtt ttgcaatccg cgccgagcga atcgcgcttg
     1441 ccctcgaggc attcgcgctc gtcccggtgc gacttggcgt tcgggcagag ccgagcgaca
     1501 tcgtcggcgc aggcgcgccg gaatccgtgt gcgtgcgttc cgcgcatgcc cagacgcatt
     1561 gcgtgatgaa gaaggggaag gcccccgtga cgcatgccga acaatccgtg gccgccatga
     1621 tggccgccgc cgccttcctg ggggccgcag ccggacgtgc tgatgcacgc aacgacaagg
     1681 atcgcggccg cattccgcaa cgaccgaagc gctcttgttt gtcggagggg attgcgcatg
     1741 gcgcgcgtgc gagttgccga tttcatcggc tggagcttat cgcagactgc cgatggaagg
     1801 cagggcggga cggggcggta ggcccatccg gtcggctccc tcgcccccga tagaggaagg
     1861 gagccgacca tcaaccgccc cttgtgattc gatttcgctt cgtccgaagc gttgaagcct
     1921 tcgcgaagac ttctccagcc tagcctttgg cgcgcccacc atgcgcgatc ggcttcagat
     1981 gtatcgcgcc agaattcgcc gccgcctcgc ggaacgtgac gtgcacgcgt ccgcgatggg
     2041 caagggcagc gtatctatgc gcgacgcggc ttttgcccgt tacagccatg cgcagcgtgt
     2101 gcacgccatg tgcgccggcc aatcgcgtgg cgcgcggctc gacgatgcca ccatgcgccc
     2161 ggatcgccgc ctgcacggag gcgagatctt cgacaacgcc ctgtttcagc cgtccgaccg
     2221 tgatgacgga tttcacgccc ggcgcatatt cgcccggcag tgaagcgcgc gccaaggtgg
     2281 cgcccgcgac gtcgatccag accggtgttc cgttcggtcg cgtaaatttc acgagctctg
     2341 cctgcgccgc gctcgcgctt cccacaacag cggcacccgc gcttcccagc gccgtcacga
     2401 tgcggctgat gtagttgcgc gtttcgtcgg gcagcaattt tcccttcatc agacgctctt
     2461 cgagatgtcc cggcccgtca ttgtaggctg cgaacatcgc cgggtagcca tacttgctgt
     2521 agagccagcg cagataggcc gcgcccgcgg caatattgtc tcccggattg taggcattgg
     2581 gtccgagacg gtattgcgtg cgcatctctt ggtaggtgcc gggctccagc tgcatgagcc
     2641 ccatcgcgcc tgtgcgcgac gtgatcgggg tgtcctcgtt cagcatcgtg cgaccgccgc
     2701 tttccatctg cacgacggcg cggatccaat ttgccggaac gctgaagcgt tgcgacgcgg
     2761 aggcgatcag cggcgtccag cggttgacga gctggctcgc gctcatgcgg ttttccgcgg
     2821 cgaacgtgga ctccggaatc tcgggcaatt gcggcactac cgcattccat gtggtgtgta
     2881 tccacttggc gaccgcggca accggagcca tcgggtcggt gcggactgag aattgctgca
     2941 cgtgaagggc gatgccctgc caggcaaaca ggatggcgcc ggcaatgccg gtcatgaagg
     3001 tcagcttggc cgcgccgtga agaacgaggc gcgcgccggc cgcctcgcat ttggttccga
     3061 aggatttgcc cgtcccggcg gtggcgccgg tagcatgaat tttcgcagaa aagaggctcg
     3121 cgccatgaag gcg
//
LOCUS       DASZMC010000383        13142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2369942,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000383 DASZMC010000000
VERSION     DASZMC010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369942"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            830..1204
                     /locus_tag="VG274_07835"
     CDS             830..1204
                     /locus_tag="VG274_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AP2/ERF family transcription factor"
                     /protein_id="HWE06604.1"
                     /translation="MHKYFADSVWGGKSKALKAARAYRDGLIATSSGAQYVLWRRNWE
                     TSRNTSGVVGVCRYVRRGRWVKGPVWQAFWNTADGVRRSRTFAVNTHGERRAKQLACE
                     ARRKGMTELRQELIRRGMIYGS"
     gene            1345..3417
                     /locus_tag="VG274_07840"
     CDS             1345..3417
                     /locus_tag="VG274_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWE06605.1"
                     /translation="MTELTSHPAGTLGTALRHAVRLLESRPELAEVQAQEILAVFPGN
                     PEALLIFSRALAAQDRLAPARDILVPLAASQPKNALVQAELGVLLGRSGDGEGAIAAL
                     ARAVEIDPHLAPAWRELGDQRLLAGDSEGADAAYAGHILASVHDPQLMEAAQALCENH
                     LAMAERLLRQFLKQHPTDTAAIRMLAETGSRLGRYEDAEKLLARCLELAPGFRAARQN
                     YATVLYRQNKAAEAIAEVDILLKDDSHNAGLRALKAAALGQVGEYARAVANYELLLEG
                     HPGQPKAWMSYGHALKALGRQKECIAAYRRSIALLPALGEAWWSLANLKTFRFSPDEI
                     ARMEAELANPRLSGEDRWHFHFALGKALEDAGEFEGSFGHYVQGNRLRRMALDHDADE
                     IASHVRRSCAFFTRELFAGRAGMGCVAPDPIFIIGLPRSGSTLIEQILASHSQVEGTM
                     ELPDIASIARRLGGKMKRSDVSTYPEVLGELEPGELRALGEEYLTRARVHRRLGRPFF
                     IDKMPNNFAHLGLIHLILPQAKIVDARRHPLACCFSIFKQHFARGQGFSYDLADIGRY
                     YADYVALMAHFDSAIPGKVLRVFYERVIDDPEREIRRLLQCLGLPFEENCLQFHRNER
                     AVRTASSEQVRQPIFKDSVDQWRFYEPWLAPLKTILAAVLAAWPDVSDIGERNGSPID
                     GGQQRGST"
     gene            3490..5931
                     /locus_tag="VG274_07845"
     CDS             3490..5931
                     /locus_tag="VG274_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWE06606.1"
                     /translation="MRGMLRRDRQGPRGALLPGLLASTMLTGVSPALAQAQGQLETVV
                     VTAQKRTENLQNVPFSIQALNTEKLEQLEVANFADYVKFLPSITYQPGGTSGGAGAGN
                     GAPGFANVYMRGVVAGGDGNHSGSLPSVGTYLDEQPVTTIGGTLNVHIYDIARVEELA
                     GPQGTLYGASSESGTIRIITNQPDSSQFEASYDVQGDSVDHGGLGYTAEGMVNYPIAD
                     NAAIRLVVWDEHDAGFIDNVPATRTYPTSGITINNFKIAKKDFNDADTIGARAALKID
                     LDSNWSVTPSIILQDEKTNGVTSADPSVGDLAVSHFYPEFVHDGWWQAALTVQGKISD
                     FDLVYAGSYMDRRIREESDYTDYSFWYDTLYGYGAYWTNNAGKPVDPSQYILAQDHFT
                     KTSHELRLTSPASDRLRFTAGLFYELQTHFILQDYRINAIGTDISVQGWPQTIWLTDQ
                     QRTDRDYAAFGEVSYDILPNLTLTGGLRVFEAVNSLEGFFGFGSGYSSHTGVSQCFAP
                     PEVDAAPCTDLNKSVTETGETHKVNLAWHIDDARMVYATYSTGFRPGGINRRGTIPPY
                     QPDTLDNYEVGWKTSWFDNRFRWNGALFYEQWNNFQYAVLGLNSFTEIHNAGQAVVKG
                     VETDVDWLIDDHLSIAGSATVADANLTTKLCGVVDPVTEQNVTQCPGPLDPYPPWAPA
                     NTQMPVTPKYKGNITARYQWNMDEFLVHLQGSLSGQSSSWADLRVEAPNPVTGVETPI
                     RAALGKQPGFAQVDLTAGVERDNWHAELFVTNAFDERGDLFNFPMCTTQICEHQVYTT
                     PITPRLIGIKFGQKF"
     gene            complement(6085..6360)
                     /locus_tag="VG274_07850"
     CDS             complement(6085..6360)
                     /locus_tag="VG274_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06607.1"
                     /translation="MKRTFVAGLVAGSMVFSSALAADPPPAVHSAAQSARSDGLQTAH
                     KTRAILRYGSATTAPCQSLSDASSTVCLLLALNVIRMSQQPLPGGAM"
     gene            6538..7545
                     /gene="fgd"
                     /locus_tag="VG274_07855"
     CDS             6538..7545
                     /gene="fgd"
                     /locus_tag="VG274_07855"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HWE06608.1"
                     /translation="MRLRLGYKASAEQFGPNELLGFGVLAEELGFDSVFVSDHFQPWR
                     HTGGHAPFSLAWLSALGAKTSRILMGTSVLAPTFRYHPAIVAQAFATLGVLFPNRIIL
                     GMGTGESMNETPATGSRWPEQKERTQRFREALDLIDTLWRDERVSFRGQFYQTSKATI
                     YDRRAEPVPIYVAGAGPVMARLAGEKAQGFICTSGKSRDLYTETLLPNLAEGLAKGGR
                     APDSIDRMIEMKVSFDTDRRRALQDTRNWGALALSQQEKHDVEDAMEMERLADALPVE
                     RTASRWIVSDDAKEHVDKLKEYLDMGFNHLVFHAPGEDQARFLKLYAEKVLPLLRSLE
                     R"
     gene            complement(7534..8103)
                     /gene="cofC"
                     /locus_tag="VG274_07860"
     CDS             complement(7534..8103)
                     /gene="cofC"
                     /locus_tag="VG274_07860"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HWE06609.1"
                     /translation="MSLRIVIPVKPFDEAKSRLAPELDADSRARLARQLFLHVFETVR
                     KFANCRNVIVVSRAAEVLTLAEAAGAIALRESSPPELNAALDQTAQFTSKSGATRILV
                     VASDLPLLSPADLAALAQGACSIAPDRHRRGTNALLWPVVLGFRFGADSFENHAAAAR
                     AAGFDPVIVLRRGLAHDVDVPDDLKDLPF"
     gene            complement(8105..8881)
                     /gene="cofE"
                     /locus_tag="VG274_07865"
     CDS             complement(8105..8881)
                     /gene="cofE"
                     /locus_tag="VG274_07865"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HWE06610.1"
                     /translation="MSEAPNKVELIALEGVPLVNAGDDLAGIVVEALARSGLWLTERD
                     VLVVAQKIVSKSEGRLVRLATVEPSPRAREVSRIVDKDPRLVEIILRESREVLRMRAG
                     VIVVEDARGLVLANAGVDASNANAIGGDESVLLLPVDPDESADRLRYGLRTLTGVEIG
                     IVINDSIGRAWRNGTIGTAIGVSGLPALLDLRGERDLFDRPLRTTDLGLADEIAAAAS
                     LLMGQAGEGRPLVLARGIPYARREGSARELSRDRKLDLFR"
     gene            complement(8995..11409)
                     /gene="cofH"
                     /locus_tag="VG274_07870"
     CDS             complement(8995..11409)
                     /gene="cofH"
                     /locus_tag="VG274_07870"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:NF005609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HWE06611.1"
                     /translation="MAIMPPPSAESLPPRDALLALMGAPLSELCSEASRLRDLGHGPI
                     ITYSRKVFIPLTHLCRDVCGYCTFAKPPRQGQRCYLNREEVLAIARTGAEAGCTEALF
                     TLGDKPELRYRQAREELAALGHPTTISWLAEMCAAVLDETGLLPHANPGVMNAHDIAR
                     LRKLTVSQGTMLENVSPRLCERGGAHFGSPDKNPDLRLETIRLAGEARVPFTSGILIG
                     IGETREERLDSLLALRDLHAAFGHIQEVIVQNFRAKPATRMAEADEPDLDDLMWTIAM
                     ARLAFGPEMNIQAPPNLSARDYGLLIDAGINDWGGVSPVTPDHVNPEAPWPQLDALRR
                     TTAAQGKTLVERLAAYPAYCVDLARWQDPGVHQQVRRHCDASGYARVDVWTPGALAPP
                     MIDLQPSRWQRQVDSGIARVIDLASAGEALAESGIERLFSARGPDFDAVCAAADALRR
                     SVSGDTVRYIVNRNINYTNVCTYKCGFCAFSKGKTSVDLRGTPYDLALEEVARRTQEA
                     WARGAVEVCMQGGIHPDYTGETYLELLRTAKSAVPEIHVHAFSPLEVTHGAATLGLSI
                     AEFLQELKQAGLGTLPGTAAEILDDEVRAVICPDKLSTDEWLNVVGTAHEIGLRTTST
                     IMYGHVETPRAWARHLLRLRGLQKRTGGITEFVPLPFVHMESPMYLRGQARRGPTFRE
                     AVLMHAVARLALHPLITNIQVSWVKMGPDGAKRCLDAGANDLGGTLMNESISRAAGTQ
                     HGQEFPAAQMDALIAALGRTPHQRTTLYAKAPPAQQRKAYRAADLTPPVQTPLRKRMR
                     HAEVEA"
     gene            complement(11566..12366)
                     /locus_tag="VG274_07875"
     CDS             complement(11566..12366)
                     /locus_tag="VG274_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWE06612.1"
                     /translation="MPITIGVSMPPQGGDMPRLRKAWEEADELGADRIYTFDHFFTLV
                     MNNPNPTFEETHGKSFEATSIQAAMAVTTKRAEISCLVHCTGYRNPNLLADISRTIDH
                     LSGGRFVLGIGSGWFKLDYDEYGYEFGTVGSRLKKLEQDIETIKDRWTKLNPPPLRRI
                     PILIGGGGEKVTLRITAKHADAWHSFGDVETMTRKSQILDEWCAKIGRNPKEIVRSTS
                     VGPLIGGIKADPEPYVKAGFSDFVVHARGPDWDLGALREILAWRKARG"
     gene            12429..>13142
                     /locus_tag="VG274_07880"
     CDS             12429..>13142
                     /locus_tag="VG274_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HWE06613.1"
                     /translation="MERAQRFRAIGREFHLSGVRVLALSGGVGGAKLLRGLYRILPAD
                     SLAAIVNTGDDFEHLGLHVSPDIDTTLYTLAGLAHPEQGWGRRDETWTFMRALKGLGG
                     ETWFALGDGDLALHVERTRRLALGLKLSLVVEDFARSFGIRAQIVPMSDDRVATRVAT
                     DEGELSFQDYFVRRHCEPVVQGLRFEGAAEARLPERAREAFASPDLEAMVIAPSNPWL
                     SIDPLLAIPELRLALQSAKC"
BASE COUNT         2467 a         4119 c         4135 g         2421 t
ORIGIN      
        1 cacacacgaa cgcgaaggtc agaatcgtcg gccagggaaa accggaggcg agaggctgca
       61 ccgttgctcg cgcgcgtttt tcgaatgcgc gcgcccgcgc gaggaaggcg tcattgcctt
      121 ccgcgggcga aggctctccc cgcgccatcg cccaatcctg aaacacttcg cgatccattg
      181 atcacgcgcc ccctgtgcgc cagccggtcg aagccgtgcc gtcagcacga gattaacaaa
      241 gagttaactc gcgggcaagt attcaaatca agcgccgact tccgccctgt tttggtcaag
      301 gacttcgact ctgcaggcaa gccgcgtctg tccccacgtt cccgccgaag gcatcgtcga
      361 ttttcaggaa ttccatgcgc gccaggggcg gcgctacatc ggcgcgtttc tcgcttcggt
      421 tcttttcgcg ctgttcgcca atatcgtcta tggcggcagc ttcaatgttt cggcatggag
      481 agcacagaac ctcgtcgtca tccccttctt gccgatcgcg accatcgaca tggtctttct
      541 cgtgcgctgg gtgcacatcg cttcgctcgt cacgatcggc gctctctgga ttttctactt
      601 cgtcgacctg cagcaggcac tgcattgacg ggcgggcgcc aagccgtgca gcctgacacc
      661 catgaccgga caaagtcgaa aaatatcttc ggcggcgcac ggcaaaacgc gccgcaagag
      721 actcactggc gggccgttca ggagcgcgcc acggcaagac cacaacatcg tccgcagcaa
      781 ggccggaagg aattactgga aggtcaaagt catgcggcgg ggcaaatcca tgcacaagta
      841 cttcgcggat tccgtctggg gaggaaaatc caaagcgctc aaagcagcga gggcctatcg
      901 cgacggtctg atcgcgacga gttcgggagc gcaatatgtg ctgtggcgcc ggaattggga
      961 gaccagccgc aacaccagcg gtgttgtggg agtctgccgc tacgtccggc gcgggcgatg
     1021 ggtaaagggt ccggtctggc aggcgttctg gaacacggcc gatggcgtgc gccgcagccg
     1081 aacctttgcc gtgaacacgc acggtgagcg acgcgccaag caactggctt gtgaggcgcg
     1141 aagaaaggga atgacggaat tacggcagga attgatccgc cgcgggatga tctacggttc
     1201 gtaatcaaat cctcgtccaa caacgactga gattccaatt atatcgaatc gggcggcatc
     1261 cgcgacgaat tcgcgggccc cacatgcgtt gacagtggca cggctgccat tctctcatcg
     1321 ggctgcgcgg cgcaggagca ttctttgacg gaactgactt cgcatcccgc gggcacgctc
     1381 gggaccgcgc ttcgtcacgc ggtgcggctg ctcgaatcgc ggccggagct ggcggaagtg
     1441 caggcgcagg agattctggc ggtctttccc ggaaacccgg aagcgctgct gattttctct
     1501 cgcgctctcg cggcgcaaga caggctcgcg ccggcgcgcg atatccttgt tccgcttgca
     1561 gcttcccagc cgaaaaacgc cctcgtacag gcggagcttg gcgtgttgct cggccgatcg
     1621 ggcgacggag aaggggcaat cgccgcgctt gcgcgcgcgg tcgagattga tccgcatctc
     1681 gctcctgcct ggcgcgagct tggcgaccaa cgcctgcttg cgggcgacag tgaaggcgcc
     1741 gacgccgcct atgcggggca catccttgca tcggtgcacg atccgcagtt gatggaagct
     1801 gcgcaggctc tctgcgaaaa ccacctcgct atggctgaac gactgctgcg ccaattcctg
     1861 aagcagcacc cgaccgacac agcggcgatt cgcatgctgg cggagacggg ttcgcggctg
     1921 ggacgttatg aggacgcgga aaagctgctc gcgcgctgtc tcgaactcgc gccgggtttc
     1981 cgcgcggcgc gccagaacta cgcgaccgtc ctctatcgcc agaacaaggc cgcggaagcg
     2041 atcgccgagg tggacattct cctcaaggat gattcgcaca atgccggatt gcgcgctctc
     2101 aaggccgctg cgctcgggca ggtcggcgaa tatgcacgcg cggtggcgaa ctatgagttg
     2161 ctgctcgagg gacatcccgg ccagcccaag gcatggatga gctacggcca cgccttgaag
     2221 gcgcttggcc gtcagaagga atgcatcgcc gcctaccgaa ggagcatcgc tttgctgccg
     2281 gctctcggcg aagcgtggtg gagtctcgcc aacctcaaga cgtttcgttt ttctccggac
     2341 gagattgccc ggatggaagc ggagctcgcg aatccccggc tcagcggcga agaccgctgg
     2401 cattttcatt ttgcgctggg caaggcgctc gaagacgcag gagaattcga ggggtcgttc
     2461 gggcattatg tgcaggggaa ccgcctgcgc cgcatggcgc tcgatcacga tgcggacgag
     2521 atcgcgagcc acgttcggcg ctcttgcgca ttctttacgc gggagctttt cgccgggcgc
     2581 gccggcatgg gctgcgtggc gcccgatccc atcttcatca tcggactccc ccgttcgggc
     2641 tcgactctga tcgagcagat tcttgccagc cattcgcagg tcgaaggcac gatggagctc
     2701 ccggacatcg cctccatcgc gcggcggctc ggtggcaaga tgaaacggag cgacgtatcc
     2761 acctatccgg aagtgctggg cgagcttgaa cccggcgaac tgcgtgcgct tggagaggaa
     2821 tatctcacgc gcgcacgcgt ccaccggcgg ctcggacgac cattcttcat cgacaagatg
     2881 ccgaacaatt tcgcgcatct gggcctgatt catctcattc ttccgcaggc gaagatcgtc
     2941 gatgcgcggc gtcatccgct cgcctgctgt ttttccatct tcaagcagca tttcgcgcgc
     3001 gggcagggct tcagctacga tctcgccgac atcggccgct attatgccga ctatgtcgcg
     3061 ctgatggcgc atttcgactc ggcgattccc ggcaaggttc tccgcgtgtt ctatgaacgc
     3121 gtgatcgatg atccggaacg ggaaattcgc cgcctgctcc aatgtttggg ccttcccttc
     3181 gaagaaaact gcctgcaatt ccaccggaac gagcgcgccg tgcgcaccgc gagctcggaa
     3241 caggtgcgcc agccgatctt caaggactcc gtggaccaat ggcgcttcta cgagccgtgg
     3301 ctcgcgccgc tgaaaacgat cctggcggcc gtcctggcag cctggccgga cgtttcggat
     3361 atcggtgaac ggaatgggtc tcccattgac gggggccagc agcgcgggtc aacatgacga
     3421 tgcgacgaca ggttcgaacc ggggacggta aagtccgaac gctgcatcgc tgatcagaag
     3481 gggatgatca tgagggggat gttgcggcgc gaccggcaag gtccgcgagg cgcgctgttg
     3541 cctgggctgc ttgcttccac catgctaacc ggtgtttcgc cggctctcgc gcaagcgcag
     3601 ggacagcttg aaacggttgt cgtgaccgcg cagaagcgga ccgagaatct gcagaacgtt
     3661 cccttcagca tccaggcgct gaacaccgaa aagctcgagc aactcgaagt cgcgaacttc
     3721 gccgattacg tgaaattcct gcccagcatc acctatcaac ctggaggaac gagcggcggc
     3781 gccggcgccg gcaatggcgc ccccggcttc gccaatgtgt atatgcgcgg cgttgtggcc
     3841 ggcggtgacg gcaatcattc gggctcgctg ccaagcgtcg gcacatatct ggacgaacag
     3901 ccggtcacca cgatcggcgg tacgctcaac gtccacatct acgatatcgc ccgcgtcgag
     3961 gaactcgcag gtccccaggg gacgctgtac ggcgccagct ccgagtcggg aacaatccgc
     4021 atcatcacca accagcccga ttcaagccag ttcgaagcca gctacgacgt ccagggtgat
     4081 agcgttgacc acggaggcct gggatacacg gcagaaggca tggtcaacta tcccatcgcc
     4141 gacaacgctg cgatccgtct tgttgtttgg gatgagcacg atgcgggctt tatcgacaat
     4201 gtgccggcca cgcgcaccta tccgacgtcc ggcatcacca tcaacaattt caagatcgca
     4261 aaaaaagatt tcaacgatgc ggatacaatc ggtgcgcggg ctgcgctgaa gatcgatctg
     4321 gactcaaact ggagcgtgac gccctcaatc atcttgcagg acgagaagac caatggcgta
     4381 acctctgccg accccagcgt cggtgatctt gcagtctcgc atttttatcc ggaattcgtg
     4441 catgacggct ggtggcaggc ggcgctgact gtgcagggca agatcagcga tttcgatctc
     4501 gtctatgccg gcagctacat ggacagacgc atccgcgagg aatcggacta cacggattat
     4561 tcgttctggt acgacactct ctacggctac ggcgcctatt ggaccaacaa cgccggaaag
     4621 cccgtcgatc cctcgcaata cattctcgcc caggatcatt tcaccaagac gagccacgaa
     4681 ctgcgtctca cctcgccggc gagcgatcgt ttgcggttta ccgcgggcct cttctacgaa
     4741 ctgcagaccc atttcatcct gcaggattac aggatcaacg ctatcggcac ggacatttcc
     4801 gttcagggct ggccacaaac gatctggttg accgatcagc agcgcacgga ccgcgactac
     4861 gcggcgttcg gcgaggtttc ctacgacatt ctgcccaatc tgacgctgac cggcggcctg
     4921 cgcgtgttcg aggcagtcaa ttcgttggaa ggatttttcg gtttcggctc aggctacagt
     4981 tcgcacacgg gcgtatcgca atgttttgcg ccgccggaag tcgatgcggc gccctgcact
     5041 gacctcaaca agtccgtgac cgagaccggc gagacgcaca aggtcaatct cgcctggcac
     5101 atcgacgacg cccgcatggt ctacgcgacc tattccacgg gattccgccc cggcggcatc
     5161 aatcgccgcg gaacgattcc tccctaccag cccgacacgc tcgacaacta cgaggttggg
     5221 tggaagacgt cctggttcga caaccggttc cgctggaacg gggctctctt ctacgagcaa
     5281 tggaacaatt tccagtacgc cgtgcttggc ctcaattcgt tcacggaaat ccataatgcc
     5341 ggtcaggcgg tcgtcaaggg cgtcgagacc gacgtcgact ggctcattga cgatcatctg
     5401 agcatcgccg gctccgccac cgtcgccgat gccaatctta caacgaagct gtgcggtgtc
     5461 gtggacccgg taacggagca gaacgtgacc cagtgtccgg gcccgctcga tccctatccg
     5521 ccctgggcgc cggcgaatac gcagatgccg gtgacgccga aatacaaggg caacatcacc
     5581 gcgcgctatc agtggaacat ggacgaattc ctcgtccatc tgcagggctc gctctcgggc
     5641 cagagcagca gctgggccga tctgcgcgtc gaagcgccca atcccgtcac cggcgtcgag
     5701 actccgatcc gtgccgctct cggcaagcag ccgggatttg cgcaagtcga tctgaccgct
     5761 ggagtcgaga gagacaactg gcacgcggaa ctgttcgtta caaatgcgtt cgacgagcgc
     5821 ggcgacctgt tcaatttccc gatgtgcacc acacagatct gcgagcacca agtctacacg
     5881 actccgatca cgccgcgcct gatcgggata aagttcgggc agaaattcta gagtacggct
     5941 cgccgtcctc ggcacgcaca atcggctctc ccgccgcgct ggtcgccaat gatgaagtgg
     6001 gtgctccgcg tacgtccggt ttgaccgtcc tgccggcgca gcgaaaagcg tgcgtcggca
     6061 gaagcagccg ggcaagcagc gaggttacat tgcaccgccc ggcaaaggct gctgcgacat
     6121 gcggatgacg ttcagcgcca gcaggaggca gaccgtcgac gatgcgtccg agaggctctg
     6181 gcatggcgcc gtagtggcgg agccgtaacg caggatggcg cgggtcttgt gcgcggtctg
     6241 caagccgtcg gatcgcgcgg attgcgcagc ggagtggaca gcagggggcg gatccgcagc
     6301 caaggcactt gaaaacacca tcgaacctgc caccaaaccc gctacgaatg tccgcttcat
     6361 cgcactgccc cgtatgctcg atacggcgcc agtctgcgcg ctcccgcttt aacggagttt
     6421 tgtgcgaatc gcgcagattc gatggcgatt tgatgcaaaa gtattcacct tttcggcgga
     6481 cctgggcgtt gtgcccgggc gggccggctc ccggccggtt tcgacggagt gaatggcatg
     6541 cgcctgcggc ttggatacaa ggcatcggcg gagcaattcg gaccgaacga gctgctgggc
     6601 ttcggagttc ttgccgaaga gctgggcttc gattccgtat tcgtcagcga ccacttccag
     6661 ccctggcgcc ataccggcgg acacgcgccg ttctctctcg cctggttgtc ggcgctgggc
     6721 gcaaagactt cgcgcatcct tatgggcacg agcgtgctcg cgccgacttt ccgctatcac
     6781 cccgccatcg tcgcgcaggc ctttgcaacg cttggcgtgc tctttcccaa ccgcatcatt
     6841 ctgggcatgg ggacgggcga atcgatgaac gagacgcccg caaccggctc ccgctggccc
     6901 gaacagaagg aacgcacgca gcgtttccgc gaggcgctcg acctgatcga cacattgtgg
     6961 cgcgacgagc gcgtctcctt tcggggccag ttctatcaaa cgtcgaaggc gaccatctac
     7021 gaccggcggg cggaaccggt gcccatctat gtggcggggg cggggccggt gatggccagg
     7081 ttggccggag aaaaagcgca gggtttcatc tgcacgagcg gaaagagccg cgatctctat
     7141 accgaaacct tgctgcccaa tttggcggag ggcctggcga agggcggtcg cgcgcccgac
     7201 tccatcgatc gaatgatcga gatgaaggtt tctttcgaca cggatcggcg gcgcgcactc
     7261 caggacaccc gcaattgggg cgcgctcgcc ctgtcgcagc aggaaaagca tgatgtcgag
     7321 gacgccatgg aaatggagcg tctcgccgat gcgctgcccg tcgagcgcac cgccagccgc
     7381 tggatcgtct ccgacgacgc caaagaacat gtggacaagc tcaaggaata tctcgacatg
     7441 ggcttcaacc acctcgtctt ccacgcaccc ggcgaagacc aggcgcgctt cctgaagctc
     7501 tacgccgaaa aagtgttgcc gctcctgcgt tcgctagaac ggtagatcct tcaaatcgtc
     7561 cggcacgtcc acgtcatggg caaggccacg gcgcaaaacg ataactggat cgaaaccggc
     7621 cgctcttgca gccgcggcgt ggttttcgaa actgtccgcg ccgaaacgaa aaccgaggac
     7681 gaccggccat agaagcgcat tggtgccgcg acgatgccgg tcgggtgcga tcgagcaggc
     7741 gccttgggcc aatgccgcga gatctgccgg actcaagaga ggcaaatcgc ttgcgaccac
     7801 gagaatcctg gttgctcccg atttgctcgt gaattgtgcc gtctgatcaa gcgcggcgtt
     7861 gagttcgggc ggcgaggatt ccctgagagc gatcgcgcca gcggcttcgg cgagcgtcaa
     7921 aacttccgcc gcgcggctga cgacgatgac gttgcggcaa ttggcaaact tccgaaccgt
     7981 ctcaaagacg tgcaggaaca gttggcgcgc caatcgcgca cgcgaatcgg catccaattc
     8041 cggcgccaga cgactcttcg cctcgtcgaa gggcttgacc ggaatgacga ttcgcaagct
     8101 cacgtcagcg gaataggtcc agttttctgt cgcgcgacag ttcgcgcgcg ctgccttcgc
     8161 gccgagcgta gggtatgccg cgagccagga cgaggggtcg gccttcgccc gcctggccca
     8221 tcagcaggga ggcagccgcg gcgatctcgt cggcaaggcc gagatcggtt gtgcgcagcg
     8281 ggcgatcgaa cagatcccgc tcgccacgca gatcgagcag ggcgggcagg ccgctcacgc
     8341 cgatcgccgt gccgatcgtt ccgttgcgcc acgcgcgtcc gatgctgtca ttgatgacaa
     8401 ttccgatttc gacgcccgtg agggtgcgaa gcccgtagcg aaggcgatcg gcgctttcat
     8461 ccggatccac gggcagaagg agaacgcttt cgtcgccgcc aatcgcgttg gcgttggacg
     8521 catcgacgcc ggcatttgcg agcaccagtc cgcgcgcgtc ctcgacaacg atcacgcccg
     8581 cgcgcatgcg caacacttcg cgagactccc gcaggatgat ctcaacgagc cgcgggtcct
     8641 tgtcgacgat gcgcgagact tcgcgtgccc gaggagaggg ttcgacggtc gccagtcgga
     8701 ccagacgccc ttccgatttg gagacgatct tctgcgcgac aacaagaaca tcgcgttccg
     8761 tgagccaaag tcccgaacgt gcgagtgctt cgacgacgat gccggccaga tcgtctccgg
     8821 cgttcacgag cggcacgcct tcgagcgcga tcagttcgac cttgttcggc gcctcgctca
     8881 tggctttcct cccccgcttg cgggggagat ccggccgagg gccggggagg ggggcagcgg
     8941 ccggaaccgc agtcccccca agcgggagag caaagcgttc ggcgtgctca tgcgctaggc
     9001 ctcgacctcg gcgtggcgca ttctcttccg cagtggagtt tgtaccggtg gggtgaggtc
     9061 ggcggcacga taggctttgc gctgctgtgc cggcggcgcc ttcgcgtaga gcgtggtgcg
     9121 ctggtgcggg gtgcggccga gcgcagcgat cagcgcatcc atttgcgcgg ccggaaattc
     9181 ctgcccgtgc tgcgtgccgg ccgcgcgcga aatgctttcg ttcatcagcg tgccgccgag
     9241 atcgttcgcc cccgcgtcaa gacaacgctt cgcgccgtcg ggtcccatct tcacccacga
     9301 cacctgaatg ttggtgatga gcgggtgcag cgccaggcgg gctaccgcat gcatcagcac
     9361 agcttcgcgg aacgtaggtc ccctccgcgc ctgaccgcga agatacatcg gcgactccat
     9421 atgcacgaaa ggcagcggca cgaattcggt gatgccgccg gttcgtttct gcaaaccgcg
     9481 caagcgaagg agatgccgcg cccaggcgcg cggcgtctcc acatgtccgt acataatggt
     9541 ggaggtcgtg cgcaggccga tctcatgcgc ggtgccgacg acgttcagcc actcgtcggt
     9601 agagagtttg tccggacaga tgaccgcgcg cacctcgtcg tccagaatct cggccgccgt
     9661 gcccggcaag gtgccgaggc ccgcctgctt caattcctgc aggaattcgg cgatcgaaag
     9721 cccgagcgtt gccgcgccat gcgtgacttc gagcggcgag aaagcatgca cgtgaatttc
     9781 cggaaccgcg gatttcgctg tgcgcaacag ctccagatag gtttcgcccg tgtagtcggg
     9841 atggatgccg ccctgcatgc agacttcgac cgcgccgcgc gcccaggcct cctgcgttcg
     9901 acgcgcgacc tcttcgagcg cgagatcgta cggcgttcca cgcaggtcga cggaggtctt
     9961 gcccttggag aaagcgcaga agccgcattt gtaggtgcaa acgttggtgt agttgatgtt
    10021 gcggttgacg atgtagcgca ccgtgtcgcc cgaaaccgaa cggcgcaatg cgtcggccgc
    10081 ggcgcaaacc gcgtcgaaat ccggtccgcg cgcggagaag agccgttcga tcccgctttc
    10141 cgcgagcgcc tcgccggccg acgccagatc gatcacgcgc gcgatgccgg aatcgacctg
    10201 ccgctgccat cggctcggct gcagatcgat catgggcgga gcgagcgcgc cgggagtcca
    10261 gacatcgaca cgcgcgtagc ccgacgcatc gcaatggcgg cgaacctgtt gatgcacgcc
    10321 gggatcctgc cagcgcgcaa gatcgacgca gtaagcagga taggcggcga gacgttcgac
    10381 gagagtcttg ccctgcgcgg cagtcgtgcg gcggagcgcg tcgagctgcg gccacggcgc
    10441 ttccggattg acgtgatcgg gcgtgaccgg cgagacgccg ccccaatcat tgatgccggc
    10501 atcaatcagc aggccataat ccctcgcgct caggttgggc ggcgcctgga tgttcatttc
    10561 cggaccgaac gcgagacggg ccatcgcgat ggtccacatc aaatcgtcga gatcgggttc
    10621 gtcagcttcc gccatgcgcg tggcgggctt ggcgcggaaa ttctggacga tgacttcctg
    10681 gatatggcca aaagccgcgt gcaagtcgcg caacgcgagc agcgagtcca gtcgttcctc
    10741 gcgcgtctct ccgatgccga tcaggatgcc cgatgtaaac ggcacgcgcg cctcgccggc
    10801 cagacggatt gtttcaaggc gcagatcggg attcttgtcg ggcgagccga aatgcgcgcc
    10861 gccgcgctcg caaagccgcg gcgagacgtt ttcaagcatc gtcccctgcg agacggtcag
    10921 cttgcgcagc cttgcaatgt cgtgggcatt catcacgccc ggattggcgt gaggcagaag
    10981 tcctgtttcg tcgagcaccg ccgcgcacat ttccgcgagc caggaaatcg tcgtcggatg
    11041 accgagcgca gcgagctcct cccgcgcctg gcgatagcgc aattccggtt tgtcgcccag
    11101 cgtgaagagc gcttcggtgc aaccggcttc cgcgccggtc cgagcaatgg cgagcacctc
    11161 ctcgcggttc agatagcagc gctggccttg cctcggaggt ttggcgaatg tgcagtaccc
    11221 gcaaacatcg cggcaaagat gggtgagcgg aatgaatacc ttgcgggaat aggtgatgat
    11281 cgggccatgc cccaaatcgc gcagtcggct ggcctcgctg cacaattcgg agagcggcgc
    11341 gcccatgaga gcaagaagcg cgtcgcgcgg tgggagggac tcagctgaag gaggcggcat
    11401 gatcgccatc atcgtcggcc aggctcgctg ccggcatttt tagcgcgcag gacgccgggg
    11461 cctcgacgag gccacagagg atttctttgc gcgtttcgcg cgccgtattt cctccgcgtc
    11521 cactgcgcat cctttgcgtc cactgcgtta aacccttccc gccgttcacc cgcgcgcctt
    11581 gcgccaggcc agaatctcgc gcagggcgcc gaggtcccag tcagggccgc gcgcatgcac
    11641 cacgaaatcg ctgaagccgg ccttcacata gggttccgga tcggccttga tgccgccgat
    11701 cagcgggcca accgaagtcg agcgcacgat ctccttcggg ttgcgcccga tttttgcaca
    11761 ccactcgtcg agaatttgcg atttgcgcgt catcgtctcg acgtcgccaa aggaatgcca
    11821 ggcatcggcg tgcttggccg tgattcgaag cgtcaccttc tcgccgccgc caccgataag
    11881 gatcgggatg cgcctgagcg gcggcgggtt cagcttcgtc cacctgtcct tgatggtttc
    11941 gatgtcctgt tcgagcttct tgagccggct gcccaccgtg ccgaactcgt agccatattc
    12001 gtcgtaatcg agcttgaacc atcccgaacc gatcccgagc acgaaacgtc cacccgaaag
    12061 atgatcgatg gtgcgcgaga tatctgccag cagattggga ttgcgatacc cggtgcaatg
    12121 gacaaggcag gagatttccg cgcgcttcgt cgtcacggcc atcgccgcct ggatggaggt
    12181 cgcctcgaaa ctcttgccgt gagtttcctc gaaggtggga ttgggattgt tcatgaccag
    12241 cgtgaagaag tggtcgaatg tgtagatgcg gtcggcgccc aactcgtcgg cttcttccca
    12301 ggctttgcga agccggggca tgtctccgcc ctgcggcggc atcgaaactc cgatggtgat
    12361 cggcatctgg gcgtgtcctt gaatggcggc ggcgttgatg ctccgcggaa tcgctgccaa
    12421 gatcaaccgt ggaacgagcg caacggttcc gcgccatcgg cagggagttc catttgagcg
    12481 gcgtgcgggt cctcgctctg tccggtggcg ttggcggcgc aaagctcctg cgcgggctct
    12541 atcgcatcct gcccgcggat tcgctcgcgg ctatcgtcaa cacgggtgac gatttcgagc
    12601 atctcgggct tcatgtttct ccggatatcg acaccacgct ctacacgctc gcagggctcg
    12661 ctcatcccga acagggctgg ggccgtcgcg acgagacctg gaccttcatg cgcgcgctca
    12721 aaggactcgg cggcgaaacc tggttcgcgc tgggcgacgg cgatctggcg ctgcatgtcg
    12781 agcgcacgcg caggctcgcg ttgggcctga agctgagcct ggtcgtggag gatttcgccc
    12841 gaagcttcgg catccgggca cagatcgttc cgatgagcga cgatcgggtg gcgacgcgcg
    12901 tcgcgacgga tgagggcgag ctctccttcc aggattattt cgtgcgccgc cattgcgaac
    12961 ctgtggtgca gggtctgcga ttcgaagggg cggcagaggc ccggctgccg gaacgcgcgc
    13021 gcgaggcctt tgcgtcgccc gatctcgaag cgatggtgat cgccccctcc aatccgtggc
    13081 tgagcatcga tccgctgctg gcaattcctg aactgcgcct ggcgctccaa tcggcgaagt
    13141 gt
//
LOCUS       DASZMC010000384         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2912139,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000384 DASZMC010000000
VERSION     DASZMC010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912139"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VG274_07885"
     CDS             <1..574
                     /locus_tag="VG274_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HWE06614.1"
                     /translation="RQIAEEFLGAWQASHPGVSVTVRDLGAEPAPHVAAEHWAAVMTP
                     AIERTDVQKSLAQASDTLIEEIEAADTVVIAAPMYNFTIPSTLKAWIDHITRAGRTFR
                     YSAQGAEGLLRGKKVFVVTARGGIYTGDSPMKSYDFHEPYLRTMLGFLGLTDVTFIHA
                     EGLNISPEAATAGMARARAAVTELVRQAAA"
     gene            709..1704
                     /locus_tag="VG274_07890"
     CDS             709..1704
                     /locus_tag="VG274_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWE06615.1"
                     /translation="MQNVAFLIERFGLLAIFLNVLLSSGGLPLPCYPALMLAAALAAP
                     AGIRIPEIVATGATATLIADTAWFWGGRRYGGAVLKLLCRISLSPDSCVRRTESIFAR
                     VGPASLTFAKFVPGLSNISVALAGATGIAFPMFLLFDTAGAVGFIGTGVALGSFFRGA
                     IATGLGFFDRLGEWSILVAAGALLLFLAWKALQRQLFLRRLRIDRITVAELRTLIDSG
                     ARSMILDVRSAEARRRGARIPGSIGEDAAMIERLCHSVAPDIEIVVYCACPNEASAAL
                     VANQLRRAGYRSARPLLGGIDAWVAAGHMIERSRDHPLPECSDACLDGSGLSQAA"
     gene            complement(1735..>2824)
                     /locus_tag="VG274_07895"
     CDS             complement(1735..>2824)
                     /locus_tag="VG274_07895"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HWE06616.1"
                     /translation="PPFIEAKRRDDSALRAKVAARPKLAKAIGDPWMEIARAQVARKA
                     LYLSYTFEEARAGFGSDLYGYARSLVRAAEERAKTNTERLPEYTDSQLPLLEKRVLDP
                     KPVYPELEQVMLEFWLSKLRENLTADAPGTKTYLGRESPEALAATLAKSDLGDPALRK
                     KLWDGGQGAVKVSADPMIRFALATDTAARAVRKDYENRVTGPTDRASEQIAKARFAAY
                     GTSTYPDATFSLRLSYGRVAGWTDNGVTVKPFTYFRGLWDRATGQFPFNLAPKWQAAR
                     GKVNDDTVFDFTADTDIIGGNSGSPVVDAKGRVVGAVFDGNIESLGGAFAFDDKVNRT
                     VAVSTAAITEALGKVYGQTALVSELSGK"
BASE COUNT          475 a          893 c          902 g          554 t
ORIGIN      
        1 ccgccagatt gccgaggagt tccttggtgc ctggcaggca agccatcccg gcgtgagcgt
       61 cactgtgcgt gatttgggcg ccgagcctgc gccccatgtc gcggccgaac attgggcagc
      121 agtgatgacg ccggcgatcg agcgaaccga cgtgcaaaaa tcgctcgccc aggcgagcga
      181 tacgctcatc gaagaaatcg aagccgccga cacggtcgtg attgccgcgc ccatgtacaa
      241 tttcaccatc ccgtcgacac tcaaggcctg gatcgatcac atcacgcggg ccggacgcac
      301 attccgctac tcggcacaag gcgcagaggg cctcctgagg ggaaagaagg ttttcgtcgt
      361 caccgcccgc ggcgggatct acactggcga tagcccaatg aagtcgtacg acttccacga
      421 gccctacctg cgcacaatgc tggggttcct cggcctgacg gacgtcacgt tcatccatgc
      481 cgaggggctg aacatcagcc ccgaagcggc gacggcgggc atggcacgcg cacgcgcggc
      541 ggttaccgaa cttgtccggc aggctgccgc ctgagccgcg ggcgcgcacc accaagcgaa
      601 ggtgcgcggc ccactgcgca ttccgcgcat tgccgctatg agaaaacagg ccttctgtgc
      661 caccgcttcg ggccgaaata ggaaacgaac aggacaggca gttcgacgat gcagaacgtg
      721 gcctttctca tcgagcggtt cgggcttctg gccatttttc tcaatgtcct gctctcgtcc
      781 ggcggccttc cgctgccgtg ctatcctgcc ctcatgcttg ccgccgcact cgcggcgccg
      841 gcgggcatcc gaattccgga aatcgtcgca accggcgcca cggccactct gatcgccgat
      901 acggcatggt tttggggagg ccggcgctat ggcggcgccg tattgaagct tctgtgccgc
      961 atttcgctgt ctccggactc ttgcgtccgc cggaccgaat cgatcttcgc gagagtagga
     1021 ccggcatcgc tcactttcgc caagttcgta cccgggcttt cgaacatctc cgtcgcgctc
     1081 gccggcgcca ccggcatcgc atttccgatg ttcctgcttt tcgataccgc cggagcggtt
     1141 ggcttcattg gaaccggggt tgccctgggc tcattctttc ggggcgcaat cgcgaccggc
     1201 ctcgggtttt tcgatcggct gggcgaatgg agcattctcg ttgccgccgg ggcgcttttg
     1261 ctgttcctgg cctggaaagc attgcagcgt cagctgtttc ttcggcgatt gcggattgac
     1321 cggatcacgg ttgcggaatt gcgcacgctg atcgattcag gcgcgcgttc catgattctc
     1381 gatgtgcggt ccgccgaggc tcgacggcgc ggggcgcgaa ttccgggctc gatcggggaa
     1441 gatgcggcga tgatcgagcg cctttgccac tccgttgccc ccgatatcga gatcgtcgtg
     1501 tattgcgcgt gccccaacga agcgtcagcg gcgctggttg caaatcagct gaggcgcgcg
     1561 ggatatcgga gcgcgcgccc gctccttggc gggatcgacg cctgggttgc ggccgggcac
     1621 atgatcgaga gatcgcgcga ccatcccctg ccggaatgca gcgacgcgtg cctggacggc
     1681 tccggattgt cgcaggcggc atgattcgcc ggcgccgaac gcacgcggcg atgtctattt
     1741 tccgctcagc tcactaacga gtgcggtctg tccgtacact ttaccgagcg cctcggtgat
     1801 ggctgccgtg gaaaccgcaa cggtgcggtt gaccttgtcg tcgaaggcaa acgcgccgcc
     1861 caggctttcg atgttgccgt cgaatacggc gccgacgacg cggcctttgg cgtccaccac
     1921 gggcgaaccg gaattgcccc cgatgatgtc ggtgtcggcc gtaaagtcga agactgtatc
     1981 gtcgttcact ttgcctcggg cggcttgcca tttcggcgcc agattgaacg ggaactgccc
     2041 cgtcgcgcga tcccacaagc cgcggaaata ggtgaaaggt ttgaccgtca cgccattgtc
     2101 cgtccagccc gcgacgcggc catagctgag gcggagcgaa aacgtcgcgt cgggataagt
     2161 gctcgtgccg tacgcggcaa agcgggcctt ggcaatctgt tcggatgcac ggtccgtggg
     2221 accggtcacg cggttctcat aatccttccg aacggcgcgc gcggccgtgt cggtggcaag
     2281 cgcgaaacgt atcatgggat cggctgaaac cttcactgct ccctggccgc cgtcccacaa
     2341 ctttttgcgc aacgccggat cgccgagatc ggatttcgcg agcgtggccg cgagcgcttc
     2401 cggcgattcc ctgccgagat acgtcttggt ccccggtgca tcggccgtga gattctcccg
     2461 cagcttcgag agccagaatt cgagcatgac ctgttcgagc tcgggataga cgggtttcgg
     2521 gtcgagcacc cgtttctcca gcaagggaag ctggctgtcg gtatactcgg gcaggcgctc
     2581 cgtgttggtc ttggcgcgtt cctcggcggc ccgcacgagc gaacgggcat agccgtaaag
     2641 gtcggagccg aatccggcgc gagcttcctc aaacgtgtac gaaagataca gagccttgcg
     2701 cgcgacctgt gcccgcgcaa tctccatcca tggatcgccg atggctttgg caagcttcgg
     2761 cctggcagcg actttggcgc gtaatgccga gtcgtcgcgg cgcttggctt cgatgaaggg
     2821 cgga
//
LOCUS       DASZMC010000385         5156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3185597,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000385 DASZMC010000000
VERSION     DASZMC010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3185597"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VG274_07900"
     CDS             <1..606
                     /locus_tag="VG274_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWE06617.1"
                     /translation="ANMQKFGEREEFRADMIRGMESATRKLGGLVGRLRPEAPDREAP
                     EIVDVAQMIAEITRQLDSTEQPVRARIATDAARVRIVPSDMHAILTHLVTNAIEASEG
                     GDEVVIGLECDCGMAVIEVEDRGSGMSAEYIRNELFVPLHSTKAQGHGMGAFQARELA
                     RAAGGEIEVVSAVGRGTSMRIVLPIIGDFPSAEPVKQAVAS"
     gene            603..1994
                     /gene="prsR"
                     /locus_tag="VG274_07905"
     CDS             603..1994
                     /gene="prsR"
                     /locus_tag="VG274_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HWE06618.1"
                     /translation="MSAEMSGVLILEDDEGLRSQYRWLLSDYRVLEAGDREAAKEIAG
                     REHPGLAIVDLGLPPDPDGASEGLAAVAELLEIAPETKIIVVTGNESREHASLAIASG
                     AYDFFQKPADPELLKLIVGRAARLYQLERENRRLAASLSQTAVEGILGASAAMQKVLR
                     DIERLARTDITVLITGESGTGKELVADAIHRLSGRAARPIVAINCAAIPEGLLEAELF
                     GHEKGAFTGAVKQTIGKIESANGGTLFLDEVGDIPLTTQVKLLRFLEERVIERVGGRQ
                     SIRIDTRVLSATNQDLAKLIAEGRFREDLFYRINEIAIPLPPLRERTGDSVLLAKFFL
                     RKYAKEFGRAFRGFSNDAASAIREHPWRGNVRELEGRVKRAAIMADGARVTAADLDLA
                     QPDTERSLNLREARKRAERRTIELALSEAEGNISKTAGLLGISRPTLYDLLEEHGFSA
                     PREALVAEPPKQR"
     gene            1999..4782
                     /gene="prsT"
                     /locus_tag="VG274_07910"
     CDS             1999..4782
                     /gene="prsT"
                     /locus_tag="VG274_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HWE06619.1"
                     /translation="MRIMSRLRHAAILMATTVGLAPVVVWAGAASSYLQSAHQLEKAN
                     DLRGAEIQLRNAAEAEPANGAIRIELAQIYLRLGNANAAQAELFAAHMRGAREETTAP
                     LMAQALLESGEFGDLLKNVPAANRPPKTESLVRTYRGMAELSLHEIDHAHAMFADAER
                     MDPRSTLPLIGEARLLLEQHQLDAAAQKVDQALKIDPRNGDALDVKGLTLAAQGKPDA
                     AMQQFAGAIAANPRNLGAMLDRASLDAQRGNLDAAEKDLQAIRKISPGSAMGFYLQAV
                     IDAQRGKFKEADALLDKLRGAMSGFPPAFLVAAEVKFRLGQLDQAQAFAQKFIAQAGD
                     QPKAYELLAGIAMKRGDVEGGIRALEKVVQIAPNDAEAMGALGEAYIAHGDLEKAKEV
                     FDRAALKAPKNAPVATARAIAHFASGDRQESVAALSDVFKGGKGSLMAGPPLVVEALQ
                     AGQIAIADQTARQLVAREPANAMYQELFAAVRIAQRDYQGAEPVLRALLDKQPSLSSA
                     RRDLAQVYLSTNRPANAKKLYQDWLRSHPADVDSLERLADIAFRENDDGEAFRLLTRA
                     QSATPADPSPSLRIVAILGVRRKWPDAISQTRTLQQRFPNDASVPDVLARLYLASGNR
                     SASLAVYKGAVARFPHIGQLFAHYAAVFAADKNYAAAAPLALRSVQLDPQNVNLKRGY
                     VSLVYLSKGPDAALAAAQSITADKNGQTAALMTADVLEGNHNRAAAIAILEKTQLRNP
                     SGILAARLAGLYQADKQSGKALALLEAWTKSHPADVDARFALAIVDSAAGRMDQALNQ
                     YEWLTVQKPHNVVILNNLAWLYDQRHDARARTVAEKAMQLAPSSSSVADTLGWIMVEQ
                     GDGVGAIKYLAQASATQPTNGTIQYHYAVVLSKTGKTAEARGVLQKLLKLDVQPDTKS
                     QAQLLLVKLGGGR"
     gene            complement(4794..>5156)
                     /locus_tag="VG274_07915"
     CDS             complement(4794..>5156)
                     /locus_tag="VG274_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWE06620.1"
                     /translation="GSSAGEFSFLTNYTKPRVGAWDYGFECSACGNGTSPPNISTLTF
                     EIKNATLSEFIQNASGYDFASDLGINCTGGPGRCYTGPVLGHPGNNQVPEPASLMLLG
                     SGLVVSGWLRRRKRTKPS"
BASE COUNT         1030 a         1543 c         1685 g          898 t
ORIGIN      
        1 gcaaacatgc agaaattcgg cgagcgcgaa gaattccgtg ccgacatgat ccgcggaatg
       61 gaaagcgcga ccagaaagct ggggggcctc gtgggccgcc tgcggcccga agcgccggac
      121 agggaagcgc cggagattgt cgatgtcgcg cagatgattg cggagatcac ccgtcagctt
      181 gatagtaccg aacagcccgt gcgggcgcgg atcgcgacgg atgcggcgag ggttcgaatc
      241 gtaccctcgg acatgcacgc gattctcacc catcttgtca cgaacgcgat tgaggcctcg
      301 gaaggcggcg acgaggtcgt cattggcctg gaatgcgatt gcggtatggc cgtgatcgag
      361 gtggaggaca ggggaagcgg aatgagcgcc gaatatatcc gcaacgaact cttcgttccg
      421 ctgcattcga cgaaggcgca aggacatgga atgggggcgt tccaggcgcg cgagcttgcg
      481 cgcgcggcag gtggggagat cgaagttgtg agtgctgtag gccgaggcac ctccatgcgg
      541 attgtgctgc caatcattgg agatttcccc tccgccgagc ccgtgaagca ggccgtggcg
      601 tcgtgagcgc agagatgtcc ggtgttctca ttctcgagga tgatgaaggc ttgcgttcgc
      661 agtatcggtg gctgttgtcg gactaccgcg tgctcgaagc cggcgaccgc gaggcggcga
      721 aggaaattgc ggggcgcgag catccggggc tcgcgatcgt cgatctgggg ttgccgcctg
      781 atcccgatgg cgcaagcgaa ggtctggcgg ccgtcgccga gcttctcgag attgctccgg
      841 aaaccaagat tatcgtcgtc accggcaacg agtcgcggga gcatgcgagc ctcgccatcg
      901 cctcgggcgc ctatgatttc tttcagaagc cggccgaccc cgagctcctg aagctgatcg
      961 tgggtcgcgc ggcgcgactc tatcagctcg agcgcgagaa ccggcgtctc gctgcatcgc
     1021 tgtcgcaaac cgcggtcgaa ggcatccttg gcgccagtgc cgcaatgcaa aaggtgcttc
     1081 gcgacatcga acgcctggcc aggacagaca tcaccgttct gatcaccggg gagagcggca
     1141 cgggaaagga gctggtggcc gacgccattc atcggctgag cgggcgcgcc gcgaggccaa
     1201 tcgtagccat caattgcgcg gccattcccg aaggactgct cgaggccgaa ttgttcggcc
     1261 acgagaaagg cgcgttcacc ggagccgtga agcagacgat cggcaagatc gaaagcgcca
     1321 acgggggcac gctctttctg gacgaggtgg gcgatattcc gctcacgacg caggtgaagc
     1381 tgttgcgctt cctcgaggag cgcgtcatcg agcgggtagg cggacggcag tcgatacgta
     1441 tcgacacacg cgtcctgagc gcgaccaacc aggatctggc aaaactgatc gccgagggcc
     1501 gtttccgcga ggatctgttt taccgcatca acgagatcgc gattcccctt ccgccgttgc
     1561 gcgagcgcac cggcgatagc gtgttgctcg ccaaattctt tctgcggaaa tatgcaaagg
     1621 agtttggccg cgcgttcagg ggtttttcca acgatgccgc gagcgcgatt cgcgagcatc
     1681 cctggcgtgg caatgttcgc gagctcgaag gccgtgtgaa gcgcgcggcg atcatggcgg
     1741 acggcgcccg ggtgacggct gcagatctcg acctggcgca gccggatacg gaacgctcgc
     1801 tcaatctgcg cgaagcccga aagcgtgcgg aacggagaac catcgaactt gctttgtcgg
     1861 aagcggaggg caatatttcg aagacggcgg ggcttctggg cataagccgg ccgaccctct
     1921 acgacctgct tgaggaacac gggttttccg cgccgcgcga agcccttgtg gcggaaccgc
     1981 ccaaacaacg gtgaaatgat gcgtatcatg tcgagactgc ggcacgccgc gatattgatg
     2041 gctacgacgg tggggctcgc gccggtagtc gtgtgggcgg gcgcggcaag ctcttatctg
     2101 caaagcgccc atcagctcga gaaggcgaat gacctccgcg gcgcggaaat ccagttgcgc
     2161 aacgcggcag aggccgaacc cgccaacggc gctatccgga tcgaactggc gcagatttat
     2221 ttgagactcg gcaatgccaa cgccgcgcag gcggaactgt tcgccgcgca tatgcgcgga
     2281 gccagggagg agacgactgc accgctgatg gcgcaggcgc tgctcgagag cggcgaattc
     2341 ggagacctgc tgaagaatgt gccggcggcc aatcggccgc cgaagacgga aagcctcgtc
     2401 cgcacctatc ggggcatggc cgagctgtcg ctgcatgaaa tcgaccacgc ccacgcaatg
     2461 ttcgccgatg ccgagcgcat ggatccccga tccacgcttc ccctcatcgg tgaagcgcgg
     2521 ctcctgctgg agcagcatca actcgacgcg gcggcgcaga aagtcgatca ggcgttgaag
     2581 atcgatcccc ggaacggcga cgcgctggat gtgaaggggc ttacgcttgc ggcgcaggga
     2641 aagccggacg cggcgatgca gcaattcgcc ggcgcgatcg ccgccaatcc ccgcaatctc
     2701 ggcgccatgc tcgaccgcgc gagcctcgat gcgcagcggg gcaatctcga tgccgccgag
     2761 aaggacctgc aggcgatccg caagatatcg ccgggcagcg caatggggtt ctatttgcag
     2821 gccgtaattg acgcgcagcg cggcaagttc aaggaggccg acgcgctgct cgacaagctg
     2881 cggggtgcaa tgagcggatt tccgccagcc tttctcgttg ctgcggaggt gaagttcagg
     2941 cttggccagc tcgaccaggc gcaggctttc gcgcaaaaat tcatcgccca ggccggggat
     3001 cagccaaaag cctacgaact gctggccggg atcgccatga agcgtggtga cgtcgaaggc
     3061 ggcatccgcg cgctcgaaaa ggtggtgcag attgctccga acgatgccga ggcgatgggc
     3121 gcgttgggag aggcctacat cgcgcatggc gatctcgaaa aggccaaaga agtcttcgac
     3181 cgggctgcgc tgaaagcgcc gaagaatgcg ccggtggcga ccgcgcgcgc gatcgctcat
     3241 tttgcgtcgg gcgaccggca ggaaagcgtc gcggcgttga gcgacgtctt caagggcgga
     3301 aaaggcagcc tgatggcagg gccgcccctc gtcgtcgagg ctttgcaggc cgggcaaatc
     3361 gcgatcgccg accagacggc ccgtcaactg gttgcgcgcg aacccgcgaa tgcaatgtac
     3421 caggaactct ttgccgccgt tcgcatcgcg cagcgggatt atcaaggcgc cgagccggtg
     3481 ctccgtgcgc ttctcgacaa gcagcccagt ctttcttccg cccggcgcga tctcgcgcag
     3541 gtctatctct ctaccaatcg gcctgcgaac gccaaaaaat tgtatcagga ttggttgcgg
     3601 tcgcatccgg ccgacgtcga cagcctggag agactcgccg acatcgcctt ccgcgagaac
     3661 gatgacggcg aagcgttccg cctgctgacc agggcccaga gtgcgacgcc tgccgatcca
     3721 agcccttcgc ttcgcatcgt cgcaatcctc ggagtccgca ggaaatggcc cgatgcgatc
     3781 tctcaaacgc ggacgttgca gcaaagattt ccgaatgatg cgtcggttcc cgatgtgttg
     3841 gcccgtctct atctcgcatc cggaaatcgc tccgcgagcc tcgccgttta caagggtgcc
     3901 gtggccagat ttccccacat cggccagctc ttcgcgcatt acgcggcggt ttttgcggca
     3961 gacaagaact acgccgccgc ggctccgctt gccctgcgct cggtccagct cgatccgcaa
     4021 aacgtcaatc tcaagcgcgg ctatgtgagt ctggtctatc tctccaaagg acccgacgcc
     4081 gcattggctg cggcgcaatc gatcaccgca gacaagaacg ggcagaccgc cgcgctcatg
     4141 accgcggatg ttctggaggg aaatcacaat cgcgcggcgg cgatcgcgat cctggagaaa
     4201 acacagcttc gtaatccttc aggaatcctt gcggcgcgac tcgccggcct ctatcaggcc
     4261 gacaaacagt ccggtaaggc attggcactg ctggaagcgt ggacgaaatc gcatccggca
     4321 gatgtggacg caagattcgc tctcgctatt gtcgacagcg cggccggaag gatggatcag
     4381 gccctcaatc agtacgaatg gctgacggtg cagaagccgc acaacgtggt gattctcaac
     4441 aatctggcct ggctctacga ccagcggcat gatgccaggg cgcggaccgt tgccgagaag
     4501 gccatgcagc tcgccccttc gtccagctcg gtcgctgata cgctgggctg gatcatggtc
     4561 gagcagggcg atggggttgg cgcgatcaaa taccttgccc aggcgagcgc gacgcagcca
     4621 accaacggca cgatccagta tcactacgcc gtcgtgctct cgaaaaccgg gaagacggcc
     4681 gaagcgcgcg gcgtgctgca gaagctcctg aagctggatg tccagcccga cacgaaatcg
     4741 caggcgcagt tgttgctggt gaagctcggc ggcggacggt aggagtcttc ttcttacgaa
     4801 ggcttcgtcc gctttcgacg tcggagccag ccggacacca caaggccgct cccgagaagc
     4861 atcagacttg cgggttctgg tacctggttg ttaccgggat gcccaaggac aggtccagtg
     4921 tagcatctcc cgggaccgcc cgtgcagttg atgcccagat ccgaagcgaa atcgtagccg
     4981 ctggcgttct gaataaattc gctgagggtg gcgttcttga tctcgaaagt cagcgtgctg
     5041 atgttcggcg gacttgtgcc gtttccgcaa gctgaacatt caaatccgta atcccaggcg
     5101 ccaaccctgg gcttggtata gttggtgagg aagctgaatt cgcccgcgct cgaacc
//
LOCUS       DASZMC010000386         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2081468,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000386 DASZMC010000000
VERSION     DASZMC010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081468"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="VG274_07920"
     CDS             <1..1713
                     /locus_tag="VG274_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWE06621.1"
                     /translation="DYHNYRRNAGTEAGHEFSAEEAHAKGLDRQTLQIEINRAALDQA
                     TTQYGLVASDAEVSNTIRAIPAFRGPLGAFDRTTFVERIRNAGYTEDSFVARERHDLA
                     TEQLLTAVHGGLAFPLDYARLFFDYLNEQRAAEYVVLPKEATGSPPQASDDELAAYVK
                     SHPVQFSTPEYREITFAVAGPQDVMNQVPVTDDQLKQQYELHKDEYQIPEKRDVEQIT
                     FPDEATAKAARAKIDAGTGFADIAKQRGLQRTDTSLGSVVADDLGKDRGPPTFALQAG
                     GVTQPIKTTFGWTLLHVTKITPGISKSFADVKDSLRKEVMNQLASAKLTDVSNAFDDA
                     SAGGAPLAQAAKRAGMRVVHVAAVDKRGLMPEGTKADLPASPDFLAQLQKADVGEEGD
                     PFPSSDGNVYVIKVNGVTPPKLKPLSSVRAEAAAAWSADVQRQRMASLADQLVAEASA
                     AKNLSGIAARLHVTVQSTGALARGAEVGTLSPQLVRNLFDAAPGSIVSAPAASGGGIA
                     IARITGIEHPPAPLGNPYFQRLVTSISSQAQGDYDLAVAMAARARVGVVINQAQLDRV
                     AGGS"
     gene            1721..>2765
                     /locus_tag="VG274_07925"
     CDS             1721..>2765
                     /locus_tag="VG274_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HWE06622.1"
                     /translation="MEFEPDFPVFSRIHDQGRGQAVFARRIADLETPVSAYMKLAGSR
                     ENAFLLESVQGGDTRGRYSVIGIDPDVVWRCRNGRAEIIGDPRFNGPATPASPDRPFQ
                     SLHTLLGETQMELPRELPPMAVGLFGYLGYDMVRHIERLGPSPPDPLDLPDALLMRPT
                     VTAIFDNIRDEIALVTPAWPRPGVDARTAYAQASQRLSGASARLDGPMPASGVDVKAT
                     AEHPIESNMTRSGYFAIVERAKAYIRAGDVFQVVPSQRFRRPFALPAFSLYRALRRLN
                     PSPFLYHLALPGFAIVGSSPEILVRLRDGTVTIRPIAGTRRRGDTPEEDATLAGELAG
                     DPKERAEHLMLLDL"
BASE COUNT          530 a          900 c          866 g          469 t
ORIGIN      
        1 gactaccaca actaccggcg aaatgcggga accgaagccg ggcacgagtt ttccgccgaa
       61 gaagcgcatg ccaaagggct ggaccgacaa accctgcaga tcgagatcaa ccgggcagcc
      121 ctggaccagg cgaccacgca atacggtctc gttgcgagcg atgccgaggt atcgaacacc
      181 attcgcgcaa ttcccgcgtt caggggaccc ctcggtgcgt tcgaccgtac aacctttgtc
      241 gagcggatca gaaacgcggg ctataccgaa gacagttttg tagcacgcga gcggcatgat
      301 ctggccaccg agcagctgct gaccgcagtc catggcggcc tcgcatttcc cctggattac
      361 gcccggctgt tcttcgatta tctgaacgag cagcgcgccg ccgagtatgt ggtcctgccg
      421 aaggaagcga cggggtctcc gcctcaagcc agcgacgacg agcttgccgc ttatgtcaag
      481 tcacatcccg tgcaattcag cacgccggaa tatcgcgaaa taaccttcgc ggtggccggt
      541 ccccaggacg tgatgaacca ggtaccggtc accgacgacc agctgaagca gcaatacgaa
      601 ctgcacaagg acgagtacca gatccccgag aagcgcgatg tggaacagat tacgtttccc
      661 gacgaagcaa ccgcgaaggc ggcgcgcgca aagatcgacg cgggaacagg tttcgcagac
      721 atagccaagc agcgcggatt gcagcggacg gacacatcgc tcgggagcgt cgtggcagac
      781 gatctcggaa aggaccgggg accgccgact ttcgcgttgc aggcgggcgg cgtgacccag
      841 ccgatcaaga ccacgtttgg ctggacactg ctccatgtta ccaagattac gcccggcatc
      901 agcaaatcct tcgcggatgt caaggattcg ttgcgcaagg aagtcatgaa tcaactcgcg
      961 agcgccaagc tgacggatgt aagcaacgcg tttgacgatg cgagcgccgg cggcgcgccg
     1021 ctcgcccagg cggccaagcg cgccggcatg cgcgtggttc atgttgccgc ggtggacaag
     1081 cggggcctga tgcccgaagg aacgaaagcc gaccttcccg cctcgccgga cttcctggcg
     1141 caactacaaa aggcggatgt cggcgaagaa ggggacccct tcccgtcctc cgacggaaat
     1201 gtctacgtca tcaaggtgaa cggcgtaacg ccgccgaagc tcaagcctct ctcttccgta
     1261 cgcgccgaag ccgcggcagc ctggtcggcg gatgtgcaac ggcagcgcat ggccagcctg
     1321 gcggatcagc tggtggccga ggcgtcggcg gccaaaaacc tgtcgggaat tgcagcgaga
     1381 ttgcatgtca ctgtccagtc gaccggcgcg cttgcacgcg gtgcggaagt cggcacgctg
     1441 tcgccacaac tggttcgcaa cttgttcgac gcagcgccgg ggagcatcgt atctgcaccc
     1501 gccgccagcg gaggcggcat cgcaatcgcg cggatcacgg gcatcgaaca tccgccggcg
     1561 cctctcggga atccctactt tcagcgcctt gtcaccagca taagcagcca ggcgcaggga
     1621 gattatgatt tggcggtcgc catggccgcg cgcgcccgtg tgggcgtcgt gatcaaccag
     1681 gcgcagctgg accgggtcgc gggcggctcg taatcgtcgg atggaattcg agccggactt
     1741 tccggtcttt tcgcgcatcc acgaccaggg tcgcggccag gccgtttttg cgcgccggat
     1801 tgcggatctg gaaactcccg tctcggccta catgaaactc gccggctcgc gcgagaacgc
     1861 attcctgctc gagagcgttc aaggcggcga cacacgtggg cgatactccg tcataggcat
     1921 tgacccggac gtcgtctggc gttgccgcaa tggacgcgcc gagatcatcg gcgatccccg
     1981 gttcaacggc cccgcaactc cggcgtcgcc ggatcgtccc tttcagtcgc tgcacacact
     2041 gctgggcgag acgcagatgg aactgccgcg cgagcttccg ccgatggccg tcggtctgtt
     2101 cggctatctg ggctatgaca tggtgcgcca catcgagcgg cttggtccct cgcccccgga
     2161 tccgctcgac ctgcccgatg ccctcctcat gcgtccaacc gttaccgcaa tcttcgacaa
     2221 cattcgcgac gaaattgcat tggtcacacc cgcatggccg cggccgggcg tcgacgcgcg
     2281 cacggcatac gcgcaggcgt cgcaacgcct gtctggggcg tccgcccgcc ttgacgggcc
     2341 gatgccggcg tccggggtcg atgtgaaggc gacggcggaa catccgatcg aatccaatat
     2401 gacccggtca gggtatttcg ccatcgtcga acgtgcgaag gcctacatcc gcgcgggcga
     2461 tgtgttccag gtggtgccaa gccagcgctt tcgcaggccg ttcgcgcttc cggccttctc
     2521 gctctaccgc gcgctccgcc gcctcaatcc gtcgcccttc ctctatcacc tcgcgctgcc
     2581 gggctttgcc attgtcggtt ccagccccga aatcctggtg cgattgcgcg acggcacggt
     2641 caccatccgc ccgattgcgg gaacgcggcg gcggggcgat acgccggagg aggacgcgac
     2701 gctcgctggc gaacttgcag gcgatcccaa ggaacgtgcg gaacatctga tgctgcttga
     2761 tctcg
//
LOCUS       DASZMC010000387         5362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1291458,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000387 DASZMC010000000
VERSION     DASZMC010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291458"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="VG274_07930"
     CDS             complement(<1..1228)
                     /locus_tag="VG274_07930"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HWE06623.1"
                     /translation="MKSPGAALTFEEVTLADVRAAAERIRGAVMRTPSHHSRTLSQIA
                     GCEIHLKFENLHFTASFKERGALNKILSLTPEERKRGIAAMSAGNHAQAVAYHAGRLG
                     IPVTIVMPEGTPFNKVKHTKALGAQIVLEGASLEAAFERARKIADAEHLVFVHPYDDP
                     LVVAGQGTVALELLEDAPDLDALVVPIGGGGLISGIALAAKALKPEIEIYGVEAKLYP
                     SMYDAVKGEQLPCAGQTIAEGIAVKRPGPLARRVVEALVKDVLLVREDEIESALVRLL
                     EIEKTVVEGAGAASLAAVLGNPELFRGRKIGVVLSGGNIDMRLLSNVVLRELAREGRI
                     LSVVIEIEDRPGLLARIATLVSDKGGNILEVSHNRMMMDMPAKSADLGLVIEARDATH
                     ADEIRSGLEAAGFTVKT"
     gene            complement(1522..1596)
                     /locus_tag="VG274_07935"
     tRNA            complement(1522..1596)
                     /locus_tag="VG274_07935"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1562..1564),aa:Met,seq:cat)
     gene            complement(1649..2776)
                     /gene="mnmA"
                     /locus_tag="VG274_07940"
     CDS             complement(1649..2776)
                     /gene="mnmA"
                     /locus_tag="VG274_07940"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWE06624.1"
                     /translation="MNGPNSLGFAKAPRDTRVVVAMSGGVDSCVVAGLLKRDGYDVIG
                     VTLQLYNHGAASKKKGACCAGQDIHDARRVAERLAIPHYVLDYEARFRERVIADFANS
                     YAKGLTPIPCVRCNEKIKFADLLDMTRDLGADALATGHYVRRVEGANGAELHRAQDAA
                     RDQSYFLFRTTNEQLQLLRFPLGGMEKRKVRAIAAGLDLAVADKPDSQDICFVPDGDY
                     ARVVQSFAADAAQPGDIVDMNGRVIGRHSGVIHFTVGQRKGLGISGETAPLYVVSLDA
                     PGARVIVGPRDALRARKIVLTDVNWLAPMAETTDCAVKVRSTREPVTARVVVKDGGCA
                     EVELPDGESAVAPGQGCVFYDGTRVLGGGWIRSAEPLSRAA"
     gene            2997..3272
                     /locus_tag="VG274_07945"
     CDS             2997..3272
                     /locus_tag="VG274_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HWE06625.1"
                     /translation="MVDERRGRASYVMGPDGSPLTMADLPAPGTKRWVIRRKAEVVAA
                     VRGGLLSLDEACRRYTLTVEEFLAWQRAIDRFGLAGLRATRVQQYRH"
     gene            3421..3816
                     /locus_tag="VG274_07950"
     CDS             3421..3816
                     /locus_tag="VG274_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06626.1"
                     /translation="MAAARAPSGVLSPWWFMGVGMSIFVDLWQSIGTVFGSFDAISFA
                     IMALVSVGAGFMMAGMGSIVTATFGALIIFALASFVRAALTAKDTQGIARSDWHDFLA
                     MPLHTLAVYALVFGVFIAIIYAVRSLGRR"
     gene            complement(4019..>5362)
                     /locus_tag="VG274_07955"
     CDS             complement(4019..>5362)
                     /locus_tag="VG274_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWE06627.1"
                     /translation="DYVRSVLMRDLAGVALPASFQPDVHILAAPEFTALCTPDGTVIV
                     TIGLLEQLENEDELAFVLGHEISHAIYRHHDSDWLARSQYYAVVNAAAVDDVAKHASF
                     AIGAANASKVARGFDVMQHLYKLSANVLAPQMTRAQEDQADSLGFDLMVRAGYDSEAA
                     LSVMDKLARQEAEAARAAQQAKAAEASSGGGGNSVSSMGSTLLGGLGKLAENGFSVSS
                     MAKNGDMTDFAFTVFDSAVDSMSDEAASHHPATEREELLSAYEFREYRDVLPVNPKPL
                     PWSPLSNSPLKPRLTALLSHYSAAEDAAGYVADAGSSTPARAQAAVQRSTAAPTHDHA
                     YTEFVASEFYGSAGKPQLSELALEKAVKGPEPSWEVYSRLLDVYMARNDYARAEALMN
                     QAVVRLDNSPVLLPKRIAILHAAGQDAEAARLLPQCNAYDIRELKSECEKAAGNG"
BASE COUNT          894 a         1738 c         1712 g         1018 t
ORIGIN      
        1 ccgtcttcac ggtaaagccc gcggcttcca gaccgctgcg gatttcgtcg gcgtgggttg
       61 cgtcgcgggc ctcgatgacg agacccagat cggcggattt cgccggcatg tccatcatca
      121 tgcggttgtg cgacacttcg agaatattgc cgcccttgtc gctcaccagc gtggcgatcc
      181 ttgccagcag ccccggccgg tcctcgattt cgatcaccac ggaaagtatg cggccctcgc
      241 gcgccagctc gcgcagcacc acgttggaga gcagccgcat gtcgatattg ccgccggaga
      301 ggacgacgcc gatcttccgc ccccggaaca attccggatt gcccagcacg gccgccagtg
      361 acgccgcgcc cgcgccttcg acaacggtct tctcgatttc gaggaggcgc acgagcgcgc
      421 tttcgatttc gtcctcgcgc accagcagga catccttgac gagcgcctct acgacgcgtc
      481 ttgcgagcgg gccgggccgc ttcaccgcga tcccttcggc aatcgtctgt cccgcgcacg
      541 gcaattgctc gcccttcacc gcgtcgtaca tcgacggata aagcttcgcc tcgacgccgt
      601 agatctcgat ctcgggcttg agcgccttcg ccgccagcgc gatgccggag atcagtcccc
      661 cgccgccaat gggcacgacg agcgcgtcga gatccggcgc gtcttccagc agctcgaggg
      721 caaccgtacc ctggccggca acgacgagcg gatcgtcgta aggatgcacg aaaacgagat
      781 gttcggcgtc ggcgattttc cgcgcccgct cgaaggccgc ttcgagcgag gcgccctcca
      841 gcacgatctg cgcgcccaac gccttggtgt gtttcacctt gttgaacggc gtgccttccg
      901 gcatcacgat ggtgaccgga atgccgagcc ggccggcgtg ataggccacg gcctgcgcat
      961 gattgccggc cgacatcgcg gcgatcccgc gcttgcgctc ctcgggcgtc agcgagagaa
     1021 tcttgttgag tgcgccgcgt tccttgaacg aggcggtgaa gtgcagattt tcgaatttca
     1081 gatggatttc gcagccggcg atctgcgaca gcgtgcgcga gtgatggctc ggcgtgcgca
     1141 tcaccgcgcc cctgatgcgc tccgccgcgg cgcgcacatc ggcgagcgtc acctcttcga
     1201 atgtcagtgc ggcgccgggc gatttcatcg catcgcttct ttctctcaac ggtaagtcga
     1261 tgcccgcgct ttcctccccc gcagtgcggg ggaggtgccc gcgaagcggg cggacgggga
     1321 cgtagcacgc gccgacacgc tctcctccgg cttgcttcgc ttcgctgagt ttatcctccg
     1381 gccgcgcttc gcgcggaccg gggggcaatc cacctccctc acacatgagg gaggaaagag
     1441 tgcttcgcgc ccggcgtccg acaatttatc cattgaagtg ggttcgtatc cgtgagtgtc
     1501 tgcttgcctc gcgcgccagc ctggtagcag cggagggatt tgaacccccg accaagggat
     1561 tatgattccc ctgctctacc actgagctac gctgccggcc ggagcgcgcg atgtaaggtg
     1621 cggccggaag gcctgtcaag gaacgaaatt acgccgcccg tgacagcggt tcggcgctgc
     1681 ggatccagcc gccgccgagg acacgcgtgc cgtcgtagaa cacgcagccc tgaccgggcg
     1741 ccaccgcgct ttcgccatcg ggcaattcca cttccgcgca gccgccatct ttcacgacca
     1801 cacgcgcggt caccggctcc cgcgtggaac ggactttcac cgcgcaatcg gtggtttcgg
     1861 ccatgggggc gagccaattg acgtccgtga gcacaatctt gcgcgcgcgc agcgcgtcgc
     1921 gcggacctac gatcacccgg gcgcccggcg catcgagaga gacgacatag agcggggcgg
     1981 tttcgccgga aatgccgaga cccttgcgct gtcccaccgt gaaatgaatc acgccggaat
     2041 ggcggccgat cacgcggccg ttcatgtcca caatgtcgcc tggctgcgct gcatcggccg
     2101 cgaagctttg caccacccgc gcatagtcgc catcgggcac gaagcagatg tcctggctgt
     2161 ccggcttgtc cgcaaccgcg agatcgagcc cggcggcaat ggcgcgcact ttgcgcttct
     2221 ccatgccgcc gagcggaaag cgcagcagct gcaattgctc attcgtggtc cggaagagga
     2281 aatagctctg gtcgcgtgcc gcatcctgcg cccgatggag ctcggcgcca ttcgcgccct
     2341 cgacgcggcg cacataatgt ccggtcgcca gcgcatcggc gccgagatcg cgagtcatgt
     2401 cgaggagatc ggcgaatttg atcttctcgt tgcagcgcac gcagggaatg ggcgtgagac
     2461 ccttcgcgta ggaattcgca aaatccgcga tcacgcgttc gcggaaccgc gcttcgtagt
     2521 cgagcacgta atgcggaatg gccagccgct ccgccacgcg ccgggcatcg tggatatcct
     2581 gtccggcgca gcacgcgccc tttttctttg acgcggcgcc gtgattataa agctgcagcg
     2641 tgacgccgat cacatcgtag ccgtcgcgct tcaggagacc cgcgacgacg caggaatcga
     2701 cgccgcccga catggcgacg acgacacggg tgtcgcgcgg cgctttggcg aagccaaggc
     2761 tgtttggtcc gttcatggcc gtgcgctata ggtcggtcgc gccggggtgg caagcgcgag
     2821 agctgcccat cacgcctgtg gtgcgcatcg cacacatatt gtcgaatcct caattgctta
     2881 tgttctcctt taccttggac cttcgcgttt ctttaaaggc ggaagcggta gcctgtcgtt
     2941 aaaggggcgg cgcgtactgt gccgttcgtg cgaacagcgt ctggagcaca atgagtatgg
     3001 tcgacgagcg caggggccgg gcgagctatg tcatgggccc cgatggaagc ccgctcacca
     3061 tggccgatct tccggctccg ggcaccaagc gctgggtgat ccggaggaag gcggaagtgg
     3121 tggccgcggt gcgcggaggc ctgttgagcc tcgacgaggc ctgccgccgt tacacgctga
     3181 cggtcgagga gtttctcgcc tggcagcgcg ccatcgatcg cttcggactg gcaggtctcc
     3241 gcgccacgcg cgtccagcaa taccgccact gacacgcgcc cggcggcacc gcgatcgcgt
     3301 tcggacaggc caatccttaa gcaagaattt acggaagtcg cgccagttat tatatgaagc
     3361 gcgcctccga gggacgctgc cggatgtgat gtcgggtccc atcctacgtg ccgagcgagg
     3421 gtggctgccg cgcgcgcgcc gtctggtgta ttgtcgccgt ggtggttcat gggggttggc
     3481 atgtccattt tcgttgatct ttggcaatcg atcggtacgg ttttcggctc gttcgatgcc
     3541 atcagtttcg ccatcatggc gctcgtttcg gtcggtgcgg gtttcatgat ggccggcatg
     3601 gggtccattg tgaccgccac gttcggggcg ctgatcatct ttgccctggc cagtttcgtg
     3661 cgtgccgccc tgacggcaaa agacacccag ggcatcgccc gttccgactg gcacgatttc
     3721 ctggcgatgc ccctgcacac gctcgccgtc tacgcgctcg tgttcggcgt attcatcgcg
     3781 atcatctacg cagtgcgctc tctcgggcgc cgctagaaag aatcacaatg agacagtgag
     3841 acagtgagaa aatatggcgc gccgacccgc ctacgccaag gcttcggccg gcgagagcgg
     3901 cagtcctccg aagctgcgga gcagcgagga ggaaggcgcg caacaattct caccacctca
     3961 ctgtctcatt gtgaatttct cttttttcgc gccttcgcaa gctgcgaaat tcgcgcgtct
     4021 agccgttccc tgccgccttt tcgcattcgg atttgagttc gcggatgtcg taggcgttgc
     4081 attggggaag gagcctggcg gcttccgcgt cctggccggc ggcgtgcagg atcgcgatgc
     4141 gcttgggcag cagcacgggt gaattgtcga gtctgacgac cgcttggttc atcagcgcct
     4201 cggcgcgcgc gtaatcgttg cgggccatgt agacatcgag caggcgcgaa tagacctccc
     4261 acgaaggttc cggacccttc accgcctttt ccagcgcaag ttccgaaagc tgcggcttgc
     4321 ctgccgagcc gtagaattcc gaggccacga actcggtgta ggcgtgatcg tgggtcggcg
     4381 cggcggtcga gcgctgcacg gcggcctgcg ctcttgccgg cgtcgacgag ccggcatcgg
     4441 cgacgtagcc cgccgcatcc tccgcggcac tgtagtggga aaggagcgcc gtcagccgcg
     4501 gcttgagcgg cgaattgctc aggggactcc aggggagcgg cttcgggttg acggggagaa
     4561 cgtcgcggta ctcgcggaac tcgtaagcgc tcaacagctc ttcgcgctcc gtcgcgggat
     4621 ggtgcgaggc cgcctcgtcg gacatggaat cgaccgccga atcgaacacc gtgaaggcga
     4681 aatccgtcat gtcgccgttc ttcgccatgc tgctgacact gaatccattc tccgccagct
     4741 tgcccagacc gccgagcagc gtgctgccca tcgaggaaac ggaattgccg cctccgccgc
     4801 tggacgcctc ggcggctttg gcctgctggg ccgcacgcgc ggcttcggct tcctggcggg
     4861 cgagcttgtc catgacggaa agcgcggctt cggaatcata gccggccctg accatcagat
     4921 cgaagccgag cgagtccgcc tggtcttcct gcgcgcgcgt catctgcggc gcaagcacat
     4981 tggcgctgag cttgtagaga tgctgcatca catcgaagcc gcgcgcgact ttcgaggcat
     5041 ttgcggcgcc gattgcaaag cttgcatgtt tcgccacatc gtcgaccgcc gcggcgttca
     5101 ccaccgcgta atattgcgac cgcgccagcc agtccgaatc atggtgccgg tagatggcgt
     5161 gcgaaatctc gtgtcccagc acgaaggcaa gctcgtcctc gttctcgagc tgctccagaa
     5221 ggccgatggt gacgatgacg gtgccgtcgg gcgtgcacag cgcggtaaac tcgggcgcgg
     5281 ccaggatgtg cacgtcaggc tggaacgatg cgggcaatgc cacgcccgcc aggtcgcgca
     5341 tcagcacgct tctgacgtag tc
//
LOCUS       DASZMC010000388         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1808780,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000388 DASZMC010000000
VERSION     DASZMC010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808780"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VG274_07960"
     CDS             <1..348
                     /locus_tag="VG274_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06628.1"
                     /translation="RVLERVDAIVARRRRIRRVVAGGVAIGFVAAAMAILGTEVLPGS
                     KTPAPRREPVYVETASDFEFPPARAGGPADALGYLLPDAAPLSRFAAEYSDATDNAGS
                     GEDVLGDGNVDLR"
     gene            500..739
                     /locus_tag="VG274_07965"
     CDS             500..739
                     /locus_tag="VG274_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06629.1"
                     /translation="MLYRFYRHKYDPTRELVVRDGAEFPHHLNAEDWYLHVTHTAVNG
                     RTEADIETLGYCDRVAGAPFGRKLSTAHHTNVMRR"
     gene            complement(808..2214)
                     /locus_tag="VG274_07970"
     CDS             complement(808..2214)
                     /locus_tag="VG274_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWE06630.1"
                     /translation="MDATRLKNFVETIWDDSVVPTLTDYIRIPNKSPAFDPEWQSHGY
                     MRDAVQLMAAWARDHLPSGATLDTVQLPGRTPLMFIEIPGASQETILLYGHLDKQPEM
                     AGWAEGTGPWTPVRVGDKLFGRGGADDGYAIFAALIAVLALRDQKQPHARCVIVIEAC
                     EESGSPDLPFYVDHLAARIGEPSLVICLDSGCGDYERLWLTTSLRGMAAGTLTVRVLD
                     EGVHSGDASGVVPSSFRILGELLARLEDQKTGGIVPAEFYVQIPPQRIEQARGAAEIL
                     GDSLYTKFPFAGTTGPVTKDLAELVLNRTWRPQLAVIGIDGLPAPQNAGNVMLPYTTA
                     RLSLRLPPTFPGAEAGELLKDLLEDAPPHGSVVAFTGSEAASGWNAPALSPWLEEAVT
                     RASMSAFGPAPAYMGEGGSIPFMAMLGEKFPRTQFVVTGVLGPHSNAHGPNEFLHIPT
                     GKRVTMAVASIVADHFHA"
     gene            complement(2291..3550)
                     /locus_tag="VG274_07975"
     CDS             complement(2291..3550)
                     /locus_tag="VG274_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE06631.1"
                     /translation="MNPSSAPLLLHPAQVWTAGEGAAREGWAALVEGGRIVDVGPLEE
                     FDGNGGAERVELPGATLFPGLIDAHSHLFLHPYSETSWDDQVLKESEAFRTLRAARHA
                     RATLEAGFTSLRDLGTEGAGYADVALQRAIEEGIIPGPRLFVATRAIVATGSYGPAAR
                     NYPGFNPEPDCCLPQGAQEASGIDEVVRAVRRQASRGADWIKLYADYRTGPDGEVRPT
                     FSQAELRAAVEAAHDSGRPVAVHAMVDEAMRRAAVAGVDTIEHGYGGSRQTFALMAEK
                     GIAFLPTLTAPEAIAEYFHHHDQASEPTENMAMAAQAFQFARDAGVTIGCGSDVGVFA
                     HGTSRRELAWMVRLGMDAADALAAATAVNAQILGRESELGRIRMGFRADLVASRGDPT
                     RDIAALSDICFVMKDGVIVCRGSTSSP"
BASE COUNT          627 a         1266 c         1195 g          634 t
ORIGIN      
        1 cgcgtgctgg aaagggttga cgccatcgtc gcgcgccgcc gccgcatccg gcgtgtcgtc
       61 gcgggcggcg tcgccatcgg ctttgtcgcc gccgccatgg cgattctcgg gacagaggtt
      121 ttgcccggga gcaaaacccc ggcgcccagg cgggaaccgg tctatgtcga gacggcttcg
      181 gatttcgaat ttccgcccgc gcgcgccggt ggaccggcgg acgcgctcgg ctacctcctt
      241 cccgatgccg caccgctgtc gcgcttcgcc gcggaatatt ccgacgccac ggacaatgcc
      301 ggcagcggcg aggacgtgct cggcgacggg aacgtagacc tccgctagcg cgcgtttcgt
      361 tcaagattcg aacaattgcg agggacgcgg gaaacgctcc gtatacgttc ttttggaact
      421 atcgggcgca gtccggcctc gcgatcccat atgtgtgcgg caaacggggc tgggcgggca
      481 cagatttgga gagcttccca tgctgtatcg attctacagg cacaaatacg atcccacccg
      541 cgaactcgtc gtccgcgacg gtgcggagtt tccccatcat ctcaacgccg aagactggta
      601 tctgcacgtg acgcacaccg ccgtgaacgg ccgcaccgaa gccgacatcg agacgctcgg
      661 ctattgcgat cgggtggcgg gcgcgccgtt cgggcgcaaa ctttcgaccg cgcaccacac
      721 caacgtgatg cgcaggtgac ggagtttcct cccccaacgt gggcgaggaa cagggagtgc
      781 ggatcgagct tcgttccgag gcgtcggtta cgcgtggaag tggtcggcga cgatcgaggc
      841 gacggccatg gtgacgcgct tgccggtggg gatgtggagg aattcgttcg gcccgtgggc
      901 gttcgaatgc gggccgagca cgcccgtcac cacgaactgg gtgcggggaa atttctcgcc
      961 cagcatcgcc atgaagggaa tgcttccgcc ttcgcccata taggcgggcg ccggcccgaa
     1021 cgccgacatt gacgcgcgcg tgaccgcttc ctccagccag ggcgacagag ccggcgcgtt
     1081 ccagccgctt gccgcctcgc tgcccgtgaa cgcaacaacg ctgccatggg gcggcgcatc
     1141 ttcgagcaga tctttcaaca gctcgccggc ctcggcgccg ggaaatgtcg gcggcagacg
     1201 caggctgagc cgcgcggtcg tatagggcag catcacattg ccggcgtttt gcggcgccgg
     1261 caggccgtcg atcccgatca ccgcaagctg cggccgccag gtgcgattga gcaccagctc
     1321 cgcgagatct ttggtgacgg ggccggtagt tccggcaaac ggaaacttcg tgtacaggct
     1381 gtcgccgaga atttccgcag cgccgcgcgc ctgttcgatg cgctgcggcg ggatttgcac
     1441 atagaactcg gcgggcacga tgccgcccgt cttctgatcc tccagccgcg ccagaagctc
     1501 gccgagaatg cgaaagctcg atggcaccac gcccgatgcg tcgcccgaat gcacgccctc
     1561 gtcgagcacg cggacggtga gcgttcccgc cgccatgccg cgcagcgacg tggtgagcca
     1621 cagccgctcg taatcgccgc aacccgaatc gagacagatg acgagcgagg gttcgccgat
     1681 gcgcgccgcc agatgatcga catagaacgg cagatcgggg cttcccgatt cctcgcaggc
     1741 ctcgatgacg atcacacagc gcgcatgggg ctgtttctga tcgcgcaacg ccagaacagc
     1801 gattaaagca gcgaatatcg catagccgtc atccgcgccg ccgcgtccga acaatttgtc
     1861 gcccacccgc accggcgtcc acgggcccgt gccctccgcc cagcctgcca tctccggctg
     1921 cttgtcgaga tggccgtaga gcaggatcgt ctcctggctg gcgcccggga tctcgatgaa
     1981 catcagcggc gtgcggcccg gcaactgcac ggtgtcgagc gtggcgccag aaggcagatg
     2041 atcgcgcgcc cacgctgcca tgagttgtac ggcatcccgc atatagccgt ggctctgcca
     2101 ctcgggatcg aaagcgggag atttgttggg gatgcggata tagtcggtga gcgtcggcac
     2161 caccgaatcg tcccagatcg tctccacgaa gttcttcagc cgtgtcgcgt ccatgttgcg
     2221 catccttgct tgcagccatc atccttcgac aagctcagga tgaggccttc atggtgagct
     2281 tgtcgaaccc tcatggtgag cttgtcgaac cacggcagac gataacgccg tctttcatca
     2341 cgaagcatat gtcgctgagc gccgcaatgt cgcgcgtggg atcgccgcgc gaggccacaa
     2401 gatcggcgcg gaagcccatg cggatccgcc ccagctcgct ttcccggccg aggatctgcg
     2461 cgttcacagc agtggcggcg gccagcgcat ccgcggcgtc catgccgagc cgcaccatcc
     2521 acgccagctc gcgccggctg gtgccgtgcg cgaacacgcc gacatcgctt ccgcagccga
     2581 tggtgacgcc tgcgtcgcgc gcgaattgaa aagcctgcgc ggccatcgcc atgttctcgg
     2641 tcggctcgct cgcctggtca tggtggtgga aatattccgc gatcgcttcg ggcgcggtca
     2701 gcgtcggcag gaacgcgatg cctttctccg ccatcagcgc aaatgtttgg cgcgatccgc
     2761 catagccgtg ctcgatcgtg tccacgcccg caaccgcggc gcggcgcatc gcttcgtcga
     2821 ccatcgcatg caccgcgacc gggcgcccgc tgtcatgcgc cgcttccacc gccgcgcgca
     2881 gctccgcttg cgaaaaggtc ggccgcactt cgccatctgg tcccgtgcgg taatcggcat
     2941 agagcttgat ccagtccgcg ccgcggctgg cctggcgccg caccgcacgg accacttcgt
     3001 cgatgccgct cgcctcttgc gcgccttgcg gcaggcagca atcgggctcc ggattaaatc
     3061 cggggtaatt tcgcgccgcc ggcccatagg acccggtggc gacgatggcg cgcgtggcga
     3121 caaagaggcg cgggccgggg atgatcccct cctcgatcgc gcgctgcagc gccacatcgg
     3181 cgtagcccgc gccctcggtg ccgagatcgc gcaaactcgt gaacccggct tcgagcgtcg
     3241 cccgcgcgtg ccgcgccgcg cgcaaggtgc gaaaggcctc cgactccttc agcacctgat
     3301 cgtcccacga ggtttcgctg tagggatgca ggaacaggtg cgaatgcgca tcgatcagcc
     3361 cgggaaacag cgtcgcgccc ggcagttcga cgcgctccgc gccgccattc ccatcgaatt
     3421 cttccagcgg ccccacatcg acgatgcgcc cgccctcgac cagcgccgcc catccctcac
     3481 gcgccgcccc ctcccccgcc gtccacacct gcgcgggatg aagaagaaga ggtgcggaag
     3541 aagggttcat gtcagcggga ccgatgataa tgcagcccga gccggtgttg atgcagcact
     3601 ccgattcccc tctcccctcg tgggagaggc gtgcaggtac gtgcatcgat ttctgctccg
     3661 gcgcgggtga ggggtcgctc gggttgcgtt atcttggcac ctatgggccc gcgcaggcta
     3721 tg
//
LOCUS       DASZMC010000389         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4838256,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000389 DASZMC010000000
VERSION     DASZMC010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4838256"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="hpnI"
                     /locus_tag="VG274_07980"
     CDS             <1..966
                     /gene="hpnI"
                     /locus_tag="VG274_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HWE06632.1"
                     /translation="EENLRSFFLQDYEGPVQIVFGARSWSDPALKVVEKLRGAFPAAD
                     VAIVVNSRLSGSNPKVANLINMVAHARHEMLVMSDSDIRVQPDYLSNVVAAMDEPRVG
                     AVSCLYSGRPVGNLWSKLAAMAIDYHFFPNARLGMALGLTEPCFGSTIAFRRQTLEEI
                     GGFEPLSNVLADDFELGRAIREKGYRLSIPASFTVEHVCSQRSARALLRQELRWARTN
                     LMLAKSGYAGTLVTYPFPLALLALLFLGLSTVSVAVVVAALASRIFLVFQSARLLGLR
                     NAHAWLLPLRDVLSFGVYAASFFGKTVFWRGTRYLASSNGALAQI"
     gene            976..2406
                     /gene="hpnJ"
                     /locus_tag="VG274_07985"
     CDS             976..2406
                     /gene="hpnJ"
                     /locus_tag="VG274_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HWE06633.1"
                     /translation="MLRTLFLQGPSFDGFDGGAGSRYQAKREVRSFWYPTWLAQPAAL
                     VEGSRLIDAPPHGLSLADILPCAKDYDLVVLHTSLPSFQSDRKTVAALKSSNPALKAG
                     FIGAKVAVDPEGSLRDAPEIDFVARNEFDFTILDVARDLAWDRIAGLSYRNGEGVIVH
                     NEERPILENMDSLPFVTPVYKRDLHIENYFIGYLKHPYISVYTGRGCKSRCTFCLWPQ
                     TVGGHRYRTRSVGHVIEEIAWAKQAFPQVREFFFDDDTFTDNLPRAEAIARELGRLGV
                     EWSCNAKANVPRASLKVMRDNGLRLLLVGYESGNQQILYNIKKGMRIDVAERFTKDCH
                     ELGVTIHGTFILGLPGETLETIEETVRFANRINPHTIQVSLAAPYPGTFLHKQAIENG
                     WLDAANAELVDERGIQIAPLHYPHLSHTQIHDSLEDFYKRFYFRWNKIGAIVGEMVRS
                     PDMMQRRLREGVEFFQFLRARREHPA"
     gene            2651..3649
                     /locus_tag="VG274_07990"
     CDS             2651..3649
                     /locus_tag="VG274_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWE06634.1"
                     /translation="MRIGVIVAALSGLALLCYLLFYVGVGPVLAAASAVGWGGFALLC
                     FYGVAMFALLCCAWFALIPSHAAPHLATFIWGRAVRDCAGEILPFSQVGGMVIGARAV
                     MLRHVSGPLASASMIVDVTMEMIAQIAFVVTGLAILIVRVPGSPSSQTLAETLAIGIV
                     VAAAGAMLFLLVQRRSLVLLERWAGRVLPQAAARAASIHRRLAEIHASPVRLGASLAI
                     HFASWLATAFWSWIAIRLIGRHIAFPSVLAIEAILSAVRSTAFIVPGAIGVQEAAYAI
                     LVPVFGLPAPVGVAISLLRRARDITLGVPILLTWQLAEGGHALKASRDAERLVTDE"
     gene            3657..>4341
                     /locus_tag="VG274_07995"
     CDS             3657..>4341
                     /locus_tag="VG274_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE06635.1"
                     /translation="MTNGDIALVTGASGFVGSAVARLLAERGFSVRVLARETSPRGNL
                     EGLDCAIVEGDMRDPDSMRAAMRDVRYLFHVAADYRLWARDPEEIVRNNVRGARVVME
                     AAAQAGVERIVYTSSVATLRPGTLEDPSDESHRLTEAQAIGAYKRSKVAAERAVEKMI
                     GEDGLPAVIVNPSTPIGPRDIKPTPTGRIIVEAASGRMPAFVDTGLNLAHVDDVALGH
                     LLALEKGRIG"
BASE COUNT          739 a         1336 c         1339 g          927 t
ORIGIN      
        1 gaggaaaatc tgcgaagctt cttcctccag gattatgaag gaccggttca aatcgtgttt
       61 ggcgcccgct cctggagcga cccggcactg aaagtggtcg agaagttgcg cggcgcattt
      121 ccagccgccg acgtggcaat cgtcgtcaat tcgcggctca gcgggtcaaa tccgaaagtg
      181 gcaaatctta tcaacatggt ggcgcatgcg cggcatgaga tgcttgtcat gagcgacagc
      241 gacatccgcg ttcagccgga ctatttgagc aatgtcgtgg ccgccatgga cgagccgcgg
      301 gtgggagcgg tgtcctgcct ctattccggc cggccggtgg gaaatctctg gtcgaaactc
      361 gcggcgatgg caattgatta tcatttcttc cccaatgcgc gcctcggcat ggctctgggc
      421 cttaccgagc cgtgtttcgg ttccacgatc gcgttccgcc gtcagacgct cgaagagatc
      481 ggcggcttcg agccgctgtc aaatgttctg gcagacgatt tcgagctcgg ccgcgccatt
      541 cgggaaaagg ggtatcgtct ttcgataccc gcatcgttca ccgtcgagca tgtatgcagc
      601 cagaggagcg cacgagcgct gctgaggcag gaattgcgct gggcgaggac gaacctcatg
      661 ctggcgaaga gcggttatgc cggaacgctc gtgacttacc cgttcccgct cgctctgctc
      721 gccctcctgt tcctgggttt atcgacggta tcggtcgcgg tcgttgtcgc agctctggcg
      781 tcgcgcatat ttctggtgtt tcagagtgca cgtttgctgg gccttcgaaa cgcgcacgcg
      841 tggctgctgc cgctgcgcga cgttctgtcg ttcggtgtct atgcggcatc gttcttcggc
      901 aagacggttt tctggcgtgg tacacggtat ctcgcaagct cgaacggcgc tctggcgcag
      961 atataggaaa tggccatgct ccgaacgctc tttcttcagg gtccctcgtt cgacggtttc
     1021 gatggcggcg caggctcgcg ctatcaggcc aagcgcgagg tgcgctcgtt ctggtatccg
     1081 acctggctcg cgcagcctgc ggcactcgtg gaaggcagcc gtctcatcga tgcgccgccc
     1141 catggactgt cgctcgcgga tattctccct tgcgcgaagg attacgacct cgtcgtcctg
     1201 catacctcgc tgccgtcgtt tcaatcggat cgcaagaccg ttgcagcgct caagagcagc
     1261 aatcctgcac tcaaggccgg atttatcggc gcgaaggtcg cggtggatcc ggaaggcagc
     1321 ctcagggacg cacccgaaat cgatttcgtg gcgagaaacg agttcgactt cacaattctg
     1381 gatgtggcgc gcgaccttgc gtgggaccgc attgctgggc tgagctatcg caacggtgag
     1441 ggcgtcatcg ttcacaacga ggagcgtccc atcctcgaga atatggattc gcttccgttc
     1501 gtgacgcccg tttacaaacg cgaccttcac atcgagaatt atttcatcgg ctatctcaag
     1561 cacccgtata tttccgtcta caccggacgc ggatgcaaat cgcgctgcac gttctgtctc
     1621 tggccgcaga cggtgggcgg acatcgctac cggacgcgaa gcgtcgggca cgtgatcgag
     1681 gagatcgcct gggccaagca ggcatttccg caggtcaggg agtttttctt cgacgacgac
     1741 acctttaccg acaatcttcc gcgcgccgag gcgatcgcca gggagctggg acggcttggc
     1801 gtcgaatggt cgtgcaacgc aaaggcgaac gtccctcgcg cgtcgctgaa ggtcatgcgc
     1861 gacaatgggc taaggctgct tctggtcggc tacgagtcgg gaaaccagca gatcctttac
     1921 aacatcaaga aaggcatgcg aatcgacgtg gccgaacggt tcaccaagga ttgtcacgag
     1981 ctgggcgtta ccattcacgg cacgttcatc ctgggattgc cgggagaaac cctcgagacc
     2041 atcgaggaga ctgtccgctt cgccaatcgc atcaatccgc acacgatcca ggtttcgctg
     2101 gcggcgccct atccgggcac atttctccac aagcaggcca tcgaaaacgg ctggctcgat
     2161 gcggcgaatg cggaacttgt cgacgaacgc ggcattcaga tcgctccact gcactacccg
     2221 catctgagtc acacgcagat ccacgattcg ctcgaggact tctacaagcg attttatttc
     2281 cgctggaaca agatcggcgc aatcgtgggc gagatggtgc gaagtccgga catgatgcag
     2341 cgccgcctgc gcgaaggcgt ggaattcttt cagttccttc gcgcccgccg cgagcatccg
     2401 gcgtgaacag cgcggtgctt gcatttccgg actttggcaa aaggccaccg cgaatttccc
     2461 ggcccggacc aattctgccc ggcagcagtg cgcacaagct ggccttgtgc cgcgcgctcc
     2521 tgaacacgtt caatccctac cgttctgccg ttctcaattg gcgcactgtc ccgacgcggg
     2581 ttcgttttgc gcgcgggttc ctgcgcaagg cgaatcgtcc catcggcacc tgatcgcgga
     2641 attttcatcc gtgcgcatcg gcgtcatcgt tgcagcgctc tcgggcctcg cgctcctgtg
     2701 ctacctgctg ttctatgtcg gcgtcggccc ggttctcgcc gcggcctctg cggtcggctg
     2761 gggtggattc gcccttctct gtttttatgg cgtggccatg ttcgcgctcc tttgctgcgc
     2821 atggtttgcg ctcattccgt cccacgcggc gccgcatctt gcgacattca tctggggacg
     2881 cgccgtgcgc gactgcgccg gtgaaattct gcctttctcc caggtcggcg gcatggtgat
     2941 cggcgcgcgg gcggtgatgc tgcgccatgt gagcggcccg ctcgcctctg cttcgatgat
     3001 cgtcgatgtg accatggaaa tgattgcgca gattgctttc gttgtaacgg ggcttgcgat
     3061 tctcattgtg cgcgtcccag gctcgccctc gagccagacg cttgccgaaa ctctcgccat
     3121 cggcatcgtt gtcgcggcgg ctggcgccat gctgtttctg ctcgttcagc ggcgaagcct
     3181 tgtgctgctt gagcgatggg cggggcgcgt ccttccacaa gccgccgccc gcgcggcgtc
     3241 gattcaccgc aggcttgccg aaattcatgc ctctccggtc cgtcttggcg cgtctctggc
     3301 gatccacttt gcaagctggc ttgctacggc cttttggtcc tggatcgcca tccgtctgat
     3361 cggcaggcac atcgcctttc catccgtgct tgccatcgaa gcgatcctgt ctgcggttcg
     3421 cagcacagcc ttcatcgttc ccggcgcgat cggcgtgcag gaagcggcct atgcgatcct
     3481 ggtcccggtt ttcggattgc cggcacctgt cggcgttgcg atatcgcttc tgaggcgcgc
     3541 gcgcgacatc accctcgggg tccccatctt gctcacctgg cagctggcgg aaggtggcca
     3601 tgcgctgaag gcgagccgcg acgccgagcg cctcgtaacg gacgaatagt ggaacaatga
     3661 cgaatggcga cattgctctg gtaacgggcg cctcggggtt cgtcggctcc gccgtggcgc
     3721 ggttgcttgc agagcgcgga ttttccgtgc gtgtgctcgc tcgcgagacg agtccgcggg
     3781 gcaatctcga ggggctcgat tgcgccatcg tcgaaggcga tatgcgcgat ccggattcga
     3841 tgcgagctgc aatgcgcgac gtgcgctatc tctttcacgt cgccgcggat tacaggctct
     3901 gggcgcgcga tcccgaagag atcgtgcgca acaatgtgcg cggcgcgcgc gtagtcatgg
     3961 aagctgccgc gcaagccggc gtcgagcgca tcgtctacac gagcagcgtc gccactttga
     4021 gacctggaac actcgaagat ccatctgacg agagccaccg cttgaccgag gcgcaggcga
     4081 tcggcgccta caagcgaagc aaggttgccg ccgagcgcgc ggtggagaaa atgatcggcg
     4141 aagacggcct tcccgccgtc atcgtgaatc cgtcgacgcc gatcggtccg cgcgacatca
     4201 aacccacgcc gacggggcgg atcatcgtcg aagccgcgtc cggccgaatg ccggccttcg
     4261 tcgacacggg actgaacctc gcccatgtcg atgatgtggc gctcggccac cttctcgcgc
     4321 ttgagaaggg acgcatcggt g
//
LOCUS       DASZMC010000390         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3471383,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000390 DASZMC010000000
VERSION     DASZMC010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471383"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="VG274_08000"
     CDS             complement(<1..1440)
                     /locus_tag="VG274_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HWE06636.1"
                     /translation="MNPDILLGEAPLTLEDVWDIAHGVRAPRLSGRADVRERLANSAA
                     HVDEAIRAGRMIYGVTTGYGDSCETRVPPELIQELPLHLTRYHRCGTGRILSPRETRA
                     VMAVRLASLMQGWSGVTPALVELLAEMLRRGVLPRIPAEGSVGASGDLTPLAYVAACL
                     AGEGVVQYRGDETPTAAAFEREGLRALALRPKEALAVMNGTAVMTGLGALAFGRAEWL
                     SRLACRLTAMASLALLGNAAHFDARLAAAKPHPGQMRATARIRVDLAQFAETCEPNRL
                     QDRYSIRCAPHVIGVLEDMLPSFRALLETEINSANDNPLFDPLDGRVLHGGHFYGGHV
                     TFAMDGLKALVANIADLLDRQLALLVDGRFNHGLPQHLSGSKAPRNAINHGLKAVQIA
                     ASAWTAEALKLTMPASVSSRSTECHNQDKVSMGTIAARDALRIVELTEQVAAVNLIAT
                     VQALRLRLRDGQLSERIIGPQIVPFLAQIG"
     gene            complement(1437..>2315)
                     /locus_tag="VG274_08005"
     CDS             complement(1437..>2315)
                     /locus_tag="VG274_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06637.1"
                     /translation="RRACLVAAFPIVLYFFVTGREQRNASRDYLDHLWRSGRLPHAPT
                     RWLSIRHFQSFAESALDKLAAWTGNIPRTTLRGESMALLDAVEASGRGAFVITAHVGS
                     PEVIRAVASLSDRVPINVLMHTEHAQLFNRLIREFSRASPVRAFPVTRVGIDTAVVLS
                     EAIARGEWVVVAGDRSPASENGRVVEAPFLGEPAAFPQGPYILGALLKAPVFLLFCAR
                     KGRDFQVHFSKFADVIELPRHDRQGAIRHYASRFAAEMEMRVTETPLQWFNFYPFWTP
                     MHEPRADEPAIKRAAE"
BASE COUNT          439 a          758 c          741 g          377 t
ORIGIN      
        1 gccgatctgg gcgagaaatg ggacgatctg cggtccgatg attcgttcgg agagctggcc
       61 atcgcgcagg cgcagccgca aagcctgcac cgtcgcaatc agattgacgg cggcgacctg
      121 ctcggtcagt tcgacgatgc gcagcgcatc gcgcgccgcg atggtgccca tcgaaacctt
      181 gtcctggttg tgacactcgg tggagcggga ggagacactg gccggcatgg tcagtttcag
      241 cgcctcggcg gtccaggccg atgctgcgat ctgcacggcc ttgaggccat gattgatcgc
      301 attgcgcggc gctttcgagc ctgaaaggtg ttgcggcagc ccgtgattga accggccatc
      361 caccagcagc gccaattggc gatcaagaag gtcggcgatg ttggcaacca gcgctttcaa
      421 tccgtccatc gcgaacgtca cgtgaccgcc atagaagtgg ccgccgtgca ggacgcggcc
      481 gtccagcgga tcgaagagcg gattgtcgtt ggcgctgttg atctcggttt ccagaagtgc
      541 gcggaacgac ggaagcatgt cctcgagaac gccgatgacg tgcggcgcgc agcgaatgga
      601 atagcgatcc tgcagacgat tcggttcgca ggtttcggcg aattgggcga ggtcgacgcg
      661 aatgcgcgcc gtcgcccgca tttgtccggg atgcggtttg gcggccgcca gccgcgcgtc
      721 gaaatgggcg gcgttcccca ggagcgccag cgacgccatg gccgtcagcc ggcaggccaa
      781 tcgcgacagc cattccgcgc ggccgaaggc gagcgcaccc aggcccgtca tgacggccgt
      841 gccgttcatc accgcaaggg cttccttggg gcgcagggca agcgcgcgca agccctcgcg
      901 ttcgaacgcc gctgcggtgg gcgtttcgtc gccgcggtat tgcacgacgc cttcgccggc
      961 aagacaggcg gccacataag ccagcggcgt cagatcgccg cttgcgccaa ccgatccctc
     1021 tgcgggtatt cgcggcagga cgccccggcg caacatctcg gccagaagct ccacgagagc
     1081 gggtgtgacg cccgaccagc cctgcatcag gctggctagc cgcacagcca tgacggcgcg
     1141 cgtttcccgc gggctgagaa tgcggccagt gccgcagcgg tgatatcgcg tcagatgcaa
     1201 cggcaattcc tgtatcagtt ccggcggcac tctcgtttcg caggaatcgc catagccggt
     1261 cgttaccccg tagatcatgc gcccggcccg gatcgcctcg tctacatgtg ccgcggaatt
     1321 ggcgagccgc tcgcgcacat ccgcccgccc ggagaggcgc ggcgcacgaa cgccgtgcgc
     1381 aatgtcccag acgtcttcga gcgtcaacgg cgcttcgccc aggagaatat ccgggttcat
     1441 tcggccgccc ttttgatcgc cggttcatcc gcgcggggct cgtgcatggg agtccagaac
     1501 ggatagaaat taaaccactg cagcggcgtc tcggttaccc gcatctccat ttcggcagcg
     1561 aagcggctcg cgtagtgccg gatcgcacct tgccggtcgt gacgcggcaa ttcgatgacg
     1621 tccgcaaatt tcgagaaatg cacctgaaag tcccggccct ttcgcgcaca aaacaaaaga
     1681 aataccggcg ccttcagaag agcacccaaa atatacggac cctgcggaaa tgccgccggc
     1741 tcgccgagaa acggcgcttc gaccacgcgg ccattctcgc tcgccggcga acggtcgccg
     1801 gcgacgacca cccactctcc ccgggcaatg gcctcggaga ggacgacggc ggtatcgatc
     1861 cctactcggg tgacaggaaa cgcccgcacg ggcgaagccc gcgagaactc ccggatcagg
     1921 cgattgaaca attgcgcatg ctcggtgtgc atcaacacgt ttatgggcac ccggtcgctc
     1981 agcgacgcga cggccctgat gacttccgga cttccgacat gcgcggtgat cacgaacgcg
     2041 ccgcggccgc tcgcctccac cgcgtcgagc agcgccatgg actcgcctct caatgtcgtg
     2101 cgcgggatat tgcccgtcca ggccgcgagc ttgtccagcg cagattcggc gaaactctga
     2161 aagtggcgga ttgacagcca acgcgtcggc gcatggggca gccgcccgct gcgccacaga
     2221 tggtcaagat agtcgcgcga agcattgcgc tgctcgcgtc ccgtcacaaa gaaatagaga
     2281 acaatcggaa acgcggccac cagacacgcg cgccg
//
LOCUS       DASZMC010000391         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5111803,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000391 DASZMC010000000
VERSION     DASZMC010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5111803"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VG274_08010"
     CDS             complement(<1..593)
                     /locus_tag="VG274_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWE06638.1"
                     /translation="MSDALTENLAKYLAVKLNAPDLTVHDLARIPGGASRETYRFRAR
                     HGGIERKLILRRDPVASLIETERSTEYRAYEAFHKIGLPVPEPIALELGTDALERPFF
                     IMAEIENAAVGSILAPDPYGAHRDRIGQQFFSILGRIAGAHPQALDLADFEGAKTPDQ
                     CWRHEVARWEKVIDEDEGEPQPIARAAIRWLKRNPPPP"
     gene            complement(590..1696)
                     /locus_tag="VG274_08015"
     CDS             complement(590..1696)
                     /locus_tag="VG274_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06639.1"
                     /translation="MLSKADDYPIHQLPEPIATAGTDRNFYDRYFFNGYTMDGSVFFA
                     AALGVYPHLNVMDASFSMIVDGVQHNLRASKLLKMERMDTAVGPISIEVVEPLKTLRL
                     RVADNEHGIKADVTFHARALPVEEPRFTYRIGPRTVMDYTRLTQNGAYDGFIEIKGTR
                     IELARDRIVGTRDRSWGVRPVGMGDPQGVAPPRLPQFYWLWSPLNFDDRFMLYHRNAD
                     ENGTPWNTASVIGALGDAAPSHMTSCASKISYKQGTRHAQRATLETVDADGGKWDVEL
                     TPRFQFYMSGIGYMHPDWGHGMYRGDSAVGYDTYDLATVNENDPRFQHIQAFVIAKLK
                     GPGSVEREGAGVLEQLVIGAYKPHGLAGIFDPAP"
     gene            1812..1887
                     /locus_tag="VG274_08020"
     tRNA            1812..1887
                     /locus_tag="VG274_08020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1845..1847,aa:Thr,seq:cgt)
     gene            complement(2008..>3176)
                     /locus_tag="VG274_08025"
     CDS             complement(2008..>3176)
                     /locus_tag="VG274_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06640.1"
                     /translation="RTEVPLCGAGHSVRMRTNDLWNQFPAFGLPTRNSQRNPMFLEQS
                     ERLRSDPQRAATYAGLRYISDEDTRGIARLSHGKGFSYRGLDGRPVDTSTLKRIRSLA
                     IPPAWTSVWISPFPDAHLAATGRDAKGRKQYRYNPEFLQIRNTAKFGHLIAFAEVLPK
                     IRKAVNGHMAMRGMPREKVIATIISLLEQTNIRIGNEGYAKENGSFGLTTLRNKHVRV
                     AGSELRFLFKGKSGKTWRLSFSNRRVAKVIRACQELPGQNLFQYEDDNGELQKVTSGD
                     VNDYLREVGERDITAKDFRTWAGTVLVAIALSSLEGPPLKKHVRDIIAHVANQLGNTA
                     TICRKCYVHPEILAAYEADELKLNIREAAANSEPGLDAGERAVLRYLKSRATNL"
BASE COUNT          583 a          927 c          985 g          681 t
ORIGIN      
        1 ggcggcggcg ggttgcgctt gagccagcgg atcgcggcgc gcgcgatggg ttgcggctcg
       61 ccttcgtctt cgtcgatcac cttttcccaa cgcgcgactt cgtgccgcca gcattggtcg
      121 ggcgtcttcg cgccttcgaa atcggcgagg tccagcgcct gcgggtgggc accggcaatg
      181 cgaccgagga tcgagaagaa ctgctgtcca atcctgtcgc gatgcgcgcc ataaggatcg
      241 ggcgcgagga tcgaaccgac cgccgcgttc tcgatctccg ccatgatgaa gaacggccgc
      301 tccagcgcat cggtgccaag ttcgagtgcg atgggctcgg gcaccggaag accgattttg
      361 tggaacgcct cgtaggcacg atattcggtc gaacgttccg tttcgatcag gctggccacc
      421 ggatcgcggc gcaggatcag cttacgctcg atgccgccat ggcgcgcgcg gaagcgataa
      481 gtctcacgcg atgcaccgcc aggaatgcgc gcgagatcgt gcaccgtgag atcgggcgcg
      541 ttcagtttta ccgccaggta ttttgcaagg ttctccgtca gcgcatcgct catggcgcag
      601 gatcaaaaat cccggcgagg ccgtgcggct tataggcacc gatgacaagc tgttcgagca
      661 cgcccgcacc ttcacgctcg acactgccgg ggcctttcag cttcgcgatg acgaacgcct
      721 ggatgtgctg gaagcgagga tcgttctcgt tgacggtcgc gagatcgtag gtgtcgtagc
      781 cgaccgcact gtcgccgcga tacatgccgt ggccccaatc gggatgcatg tagccgatgc
      841 cggacatgta gaactggaag cgcggcgtca gttcgacgtc ccacttgcca ccatcggcat
      901 cgacggtttc gagcgtagca cgttgcgcat gccgcgtgcc ctgcttgtag gaaatcttcg
      961 acgcgcagct tgtcatgtgc gacggcgcgg catcgccgag tgcgccgatc accgaggcgg
     1021 tgttccacgg cgttccgttc tcgtcggcgt ttctgtgata aagcatgaaa cggtcgtcga
     1081 aattcagcgg cgaccacagc cagtagaatt gcggcagacg cggtggcgcc acgccttgtg
     1141 gatcgcccat gccgacgggg cgcacgcccc aggaacggtc gcgcgtgcca acaattctat
     1201 ctcgcgccag ttcgatccgt gtgcctttga tctcgatgaa gccgtcatag gcgccgttct
     1261 gtgtcagacg cgtgtagtcc atcaccgtgc gcggaccgat gcgataggtg aagcgtggct
     1321 cctcgaccgg caaggcgcgc gcatggaacg tcacatcggc tttgatgcca tgctcattgt
     1381 cggcgacgcg caagcgcaag gtcttgagtg gctcgacgac ctcgatagag atcgggccca
     1441 ccgccgtatc catccgttcc attttgagaa gcttcgaggc acgcagattg tgctgcaccc
     1501 cgtccacgat catggagaac gaggcgtcca tcacattcag gtgcgggtag acgcccaagg
     1561 cggccgcgaa gaacacgctg ccgtccatcg tgtagccgtt gaagaagtag cggtcgtaga
     1621 aattgcggtc ggtgccggcc gtcgcgatcg gctcgggcag ctggtggatt ggatagtcgt
     1681 cggctttgga gagcatgcgg cagcttgacc cgtccagacg tgcccggcaa cgcgaaaaac
     1741 gaggctttcc gcggcgtaaa tcctctgttg cagtatcggc ggcttctgcc tattttccgc
     1801 gccttcccgg ggccgcttta gctcagccgg tagagcacat cattcgtaat gatggggtcg
     1861 cgtgttcgag tcacgcaagc ggcaccaagt cccttcgatg cagtaacagc aaagcaactc
     1921 gatctgccgc ttggtgggct aggcgctctc ctgctgcaca gagcctcgac gcaccacatg
     1981 gtctgtcgca ctttcctctc ggacttctta gaggttggtg gcacgggatt ttagatatct
     2041 caggacagcg cgttctccgg catctaggcc aggttcactg ttggccgctg cttctcgaat
     2101 gttcagtttg agttcatcag cctcgtaggc tgccaggatt tcgggatgca cgtagcattt
     2161 cctgcaaatg gtggctgtgt tacccagttg attggcgacg tgggcgatga tatcgcggac
     2221 gtgcttcttg agaggagggc cttcaagact gctgagagcg attgctacca gaaccgtacc
     2281 ggcccaggtc cgaaagtcct tggctgttat atcgcgctca ccgacctcgc gaaggtagtc
     2341 gttcacgtcg cccgacgtca ccttctgaag ctcgccgttg tcatcctcgt actgaaaaag
     2401 attctgcccc ggcagttcct gacacgcacg aatgactttc gccacacggc gattgctgaa
     2461 ggaaagacgc caggtttttc cgctctttcc cttgaacaga aatcgcaact ccgaaccggc
     2521 gacacgcaca tgtttgttgc gaagcgtcgt aaggccgaaa cttccgttct ctttcgcata
     2581 gccttcgttg ccgatgcgga tattcgtctg ctccagcagc gagatgattg tcgctatcac
     2641 tttttcgcgc ggcatgccgc gcatcgccat atggccgttc acggccttgc gtatctttgg
     2701 gaggacctcg gcaaaggcga tcagatggcc aaacttcgcc gtgtttcgga tctgcaggaa
     2761 ctcgggattg tagcggtatt gtttgcgccc ctttgcatcg cgcccggttg cggcaagatg
     2821 ggcgtccggg aacggcgaaa tccataccga ggtccatgcc ggtgggatgg ccagtgatcg
     2881 aatgcgtttc agggtcgacg tgtcgacggg tcgtccgtcg agaccgcgat aggaaaagcc
     2941 tttgccgtgc gataggcggg cgattccgcg tgtgtcttcg tcggaaatgt agcgcaatcc
     3001 ggcgtaagtc gcggcacgct gcgggtccga cctgagccgc tcgctttgct ccaggaacat
     3061 agggtttcgc tgagagttgc gggtaggaag cccgaatgcg ggaaattggt tccacaaatc
     3121 gtttgtacgc attcggacgg agtgtcctgc accgcacagc ggaacttcgg tgcgtg
//
LOCUS       DASZMC010000392         6263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1293075,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000392 DASZMC010000000
VERSION     DASZMC010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293075"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..720
                     /locus_tag="VG274_08030"
     CDS             211..720
                     /locus_tag="VG274_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06641.1"
                     /translation="MTFDPTIYGPAIASLLALDSNGQRLIPLVQSSPSHAEAKAQIDS
                     LHAPPPVRAGLYLYFSLWSQAHETAQDINSPEGAYWHAIVHRQEPDASNSGYWFRQVG
                     EHPIFPALRDQAVSLGVDLGLRWSPTRFIEFCEQARQQPGSDAERRALEVQRAEWQLL
                     FDYCNSHSR"
     gene            complement(755..937)
                     /locus_tag="VG274_08035"
     CDS             complement(755..937)
                     /locus_tag="VG274_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06642.1"
                     /translation="MKLDVEHLEVENTRCSNGDDGDGLIEIGGLEALAQLFAGNRGEL
                     RAATIDALDFALDATA"
     gene            878..2827
                     /locus_tag="VG274_08040"
     CDS             878..2827
                     /locus_tag="VG274_08040"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06643.1"
                     /translation="MVAVAASRVLHLEMFDVQLHGALALAQGRIAEMQTGEGKTLAAT
                     PAVAWLAREHSGVHVMTVNDYLARRDAQWMGGIYRLLGFSVGCIQQSATPEERRRAYA
                     CDITYATANEIGFDYLRDNLALHLEDRVHRPFHAAVIDEADSILIDEARIPLVLAGGE
                     EDEESLARRADALGGSFRHGFDFTLDEFGRNISLTAAGAHQAEAAFHIDNLYEEENLA
                     IYTAVHDAIHAHHLLRRDVDYVVKDSAIESVDQFKGRIAQDRRWPAGLHTALEAKERL
                     PFRKQGRVLATITLQNLAALYPNLSGMTGTAATQASDFLKFYNLPVEVIPPNRPVIRA
                     DEPDRLFRSRFAKEEAVLEEIHEVHACGQPILVGTSSVEESERLSAKLFDVPHSVLNA
                     RNEEEEASIIAQAGQRGAVTISTNMAGRGVDIQLGPGVAELGGLYVLGMNRHESRRID
                     HQLRGRAGRQGDPGRSRFFISTEDDLLARYAGDDASFPGAEALQRRVEGQNLETRLFL
                     DKYESAIEGQRHKIQQHRDAILSSDDPEFERLVALRTIDDLWSMYLETVAELRSGIHW
                     HAYTGHDPLYNFLTSIDQLFRELEENLDAEIAQRLEEARTTGFDPSQRGATWTYLTTD
                     NPFGDLTQRMAKGIARKIAKKQLWG"
     gene            complement(2844..3329)
                     /locus_tag="VG274_08045"
     CDS             complement(2844..3329)
                     /locus_tag="VG274_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06644.1"
                     /translation="MPQYTCSTADCTSGGAPIVLNQANQTFCPQCGNGTLVAYVPPPM
                     EQMVNAGGTMTTANPTQANDCNALINLFGVTLVSVDEARMPRDCIVKLGADRRKRIDR
                     QGPVSTGNTVTHARFAVQLGDPIYAGIHMPVDTNFTANHVRAAFRRSLQQHQYVRIFW
                     G"
     gene            3513..4376
                     /locus_tag="VG274_08050"
     CDS             3513..4376
                     /locus_tag="VG274_08050"
                     /inference="COORDINATES: protein motif:HMM:NF007695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWE06645.1"
                     /translation="MQQQTTRPGGEFQIGGDLAVARLGYGAMRITGPGIWGEPANRAD
                     AIRVLRRAIELGVNFIDTADSYGPAVSEEIIAEALHPYPKGLVIATKGGFDRPGPNRW
                     VENGKPEHLRAACDSSLRRLRLERIDLYQLHRIDSKYPAEDQLGTLKDLQSQGKIRHI
                     GLSEVSVRQIEHARSIVPIVSVQNRYSLTDRASEDVLECCERESIGFIPWFPLAAGRL
                     SGRESVIQSAAEKLKATPSQLALAWLLQRSPVMLPIPGTSSIPHLEENVAAANLQIPP
                     DVMHELDQLNR"
     gene            complement(4396..5418)
                     /locus_tag="VG274_08055"
                     /pseudo
     CDS             complement(4396..5418)
                     /locus_tag="VG274_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
     gene            5599..>6263
                     /locus_tag="VG274_08060"
     CDS             5599..>6263
                     /locus_tag="VG274_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWE06646.1"
                     /translation="MLDRFIYGSVSRISPEAPVPVLQIREQHSMLGGAGNAVRNLHAL
                     GCSVRFTSVVGEDAAAAEIESALVAMPKCQWFLAYELGRQTIVKTRYIADSQQVMRAD
                     AESKHEIAAEPFAAALERFQATLEGASVVLLSDYGKGLLTAGRAHAFIDLARARGIPS
                     IVDPKGAAFRRYNGATVIKPNLKELAEATGMPVDTDQAREAAARALLADLDIEHLLVT
                     CGAS"
BASE COUNT         1204 a         2217 c         1663 g         1179 t
ORIGIN      
        1 ttcagacacc ggcgccgcgc cagccaccgc gcagcacaat cgccccgtcc acccctcaaa
       61 tccaccccga aaggagaatt ccgccgcctg aaaccgatga aaaatcgatc gacccaagcc
      121 gaaaacatta gcccgtcacc cgcagtgtgt ccgcttgccc cgagaagctg gcgcatggcg
      181 ccccctcccg cgctgtccca taattggagc atgacgttcg accccaccat ttacggtccg
      241 gccatcgcct ccctcctcgc cctggattca aacggccagc gtctcatacc cctagtccaa
      301 tcgtcccctt cgcacgccga agccaaggcc caaatcgact cgctccacgc cccgccgccc
      361 gtccgcgcag gcctgtacct ctacttttcc ctctggtcgc aagcgcacga aaccgcgcag
      421 gacataaact cgccggaggg cgcctactgg cacgccatcg tccaccgcca ggaacccgac
      481 gcctcgaact caggctactg gttccgtcag gtcggcgaac atcccatctt ccccgcgctt
      541 cgcgatcagg ccgtgtccct tggcgttgac ttaggccttc gctggagccc cactcgcttc
      601 atcgaattct gcgaacaggc ccgccagcag cccggcagcg acgccgaacg ccgcgccctg
      661 gaggttcagc gcgccgagtg gcaattgctc ttcgactact gcaactccca ctcgcgttga
      721 tcgtccccac tgtatgcgcc tctttgaaca tctcctaagc ggtggcgtcc agcgcgaaat
      781 ccagcgcatc gatcgtcgcc gcgcggagct cgcctcggtt tccggcgaag agctgcgcca
      841 acgcttccag tccgccgatc tcgatgaggc catcgccatc gtcgccgttg ctgcatcgcg
      901 tgttctccac ctcgagatgt tcgacgtcca acttcacggc gccctcgcgc tcgcgcaggg
      961 ccgcatcgcc gaaatgcaaa ccggtgaagg gaagacgctc gccgccactc ccgccgttgc
     1021 ctggttggcc cgcgaacaca gcggcgtcca cgtcatgacc gtcaacgatt acctcgcgcg
     1081 ccgcgatgct caatggatgg gcggcattta ccgcctgctc ggtttttccg tcggctgtat
     1141 tcagcaaagc gctacgcccg aagaacgccg ccgcgcctac gcctgcgaca tcacctacgc
     1201 caccgctaat gagatcgggt tcgattacct gcgcgacaac ctggctctgc atctcgaaga
     1261 tcgcgtccac cgccccttcc acgccgccgt catcgatgag gccgattcca tcctcatcga
     1321 tgaggcgcgc atcccgctcg tactcgccgg cggtgaagag gatgaggaat ccctcgcacg
     1381 ccgcgccgat gcgttgggag gctccttccg ccatggcttc gatttcaccc tcgatgagtt
     1441 cggccgcaac atttcgctga cagccgcggg cgcgcatcag gccgaagccg cctttcatat
     1501 agacaatcta tatgaagaag agaatctcgc catctacacc gccgttcacg acgccatcca
     1561 cgcccaccac ctcctgcgcc gcgatgtcga ttacgttgtt aaagactccg ccatcgaatc
     1621 tgtcgatcag ttcaaaggcc gcattgcaca ggatcgccgc tggccagccg gtctgcacac
     1681 cgcgctcgaa gccaaagagc gccttccgtt tcgtaaacag ggccgcgtcc tcgccaccat
     1741 caccctgcaa aatctcgccg cgctctatcc caatctctct ggcatgaccg gcaccgccgc
     1801 cacccaagcc agcgacttcc tcaagttcta caacctcccc gtcgaagtca ttccgcccaa
     1861 tcgccccgtg attcgcgccg atgagcctga tcggctcttc cgctcccgct tcgccaaaga
     1921 ggaagccgtc ctcgaagaga tccacgaagt ccatgcatgc ggtcagccca ttctcgtcgg
     1981 gacgtccagc gtcgaagagt cggaacgcct cagcgccaag ctattcgacg ttccgcactc
     2041 ggtgctcaac gcgcgcaacg aagaggaaga ggcctccatc atcgcgcaag ccggccagcg
     2101 cggcgccgtc accatctcca ctaacatggc gggccgcggc gtcgatatcc aactcggccc
     2161 cggcgtcgcc gaactcgggg gactctacgt tctcggcatg aaccgtcacg aaagccgtcg
     2221 catcgaccac cagctccgcg gccgcgccgg acggcaaggc gaccccggcc gctcccgctt
     2281 cttcatctct actgaggacg atctgctcgc ccgttacgct ggcgacgacg cgtccttccc
     2341 cggcgctgaa gcccttcaac gtcgcgtcga aggacagaac ctcgaaacgc gtctcttcct
     2401 cgataaatac gaatccgcca ttgagggtca gcgtcacaaa atccagcagc atcgcgacgc
     2461 catcctctcc tccgatgatc cggagttcga acgcctggtc gcgcttcgca ccatcgacga
     2521 tctctggtcg atgtatctcg aaaccgttgc cgaactgcgc tccggcatcc actggcacgc
     2581 gtacaccggg cacgatccgc tctacaactt cctcaccagc atcgaccagc tctttcgcga
     2641 actcgaagaa aatctcgacg ccgaaatcgc ccaacgcctc gaagaggcgc gcactaccgg
     2701 cttcgatccc tcgcagcgcg gcgccacctg gacctatctc accaccgaca atcccttcgg
     2761 cgatctcacc cagcgcatgg ccaaaggcat cgcgcgtaag atcgccaaga agcaactgtg
     2821 gggatgagtg gcgattaagg tcgctagccc cagaaaatac gcacgtactg gtgctgctgc
     2881 aaacttctgc ggaaggcggc ccgcacgtga ttcgccgtga aattcgtgtc gaccggcatg
     2941 tgaatgcccg cgtaaatagg atcgcccagt tgcactgcaa aacgcgcgtg agtgactgtg
     3001 ttgccggtgg aaacggggcc ttgacgatcg atgcgtttcc gtcgatcagc tcctaacttc
     3061 acaatgcaat ctctcggcat acgagcttcg tcgactgaca ctaacgtgac gccgaacagg
     3121 ttaatcaggg cgttgcagtc attcgcttgg gttgggttcg cggttgtcat tgtgcccccg
     3181 gcatttacca tttgctccat cggcggcggc acataagcca caagcgtgcc gtttccgcat
     3241 tgcggacaaa atgtctgatt ggcctgattt aaaacaatcg gcgccccacc ggacgtgcaa
     3301 tcggccgtcg agcatgtgta ttgcggcatg aatcctcctc gagcattata ctcaggctct
     3361 tctgctgggg atgattgcac acgctgcggc agccccactc gcgaatcatt ctcgcaactg
     3421 tggccacatc gctattgcaa ctgccgcccg cttcgttaac ctacgaagag acgcaagcgg
     3481 ccgaactgtt ccgcgcgtaa ggaggttaca tcgtgcagca acagacaacc cgccccggcg
     3541 gtgaatttca aatcggcggc gatctcgcgg tcgctcgtct cggctacgga gccatgcgca
     3601 tcaccggccc cggcatatgg ggagagcccg ccaatcgcgc cgacgccatc cgtgttctgc
     3661 gccgcgccat cgaactcggc gtcaacttca tcgatactgc ggattcctac gggcccgccg
     3721 tctccgaaga gatcatcgcc gaagcgctgc atccgtatcc gaaaggtctc gtgatcgcaa
     3781 ccaaaggtgg cttcgaccgg cccggtccca atcgttgggt ggagaacggc aagcccgaac
     3841 acctccgcgc cgcttgcgac agcagcctgc gccggctgcg cctggagcgc atcgacctgt
     3901 atcagttgca ccgcatcgat tcgaagtacc ctgctgaaga tcagctcggc acgctcaaag
     3961 acctgcagtc gcagggcaaa attcgccaca tcggtctttc cgaagtcagc gtgcgtcaaa
     4021 tcgagcacgc ccgctccatc gtcccgatcg tcagcgtgca gaatcgctac agcctcaccg
     4081 accgcgcctc cgaagacgtg ctcgaatgct gcgagcgcga atccatcggc ttcattccct
     4141 ggttccctct cgccgccggg cggctctccg gacgcgaaag cgtcattcag agcgccgccg
     4201 aaaagctcaa agcaacgcct tcgcaactcg ctctcgcgtg gctcctccag cgttcgcccg
     4261 tgatgctgcc gattcccggg acgtcgagca ttccgcatct cgaagagaac gtcgcggctg
     4321 cgaatctgca gattccgccg gacgtgatgc atgagctaga tcaactgaat cgctagccgg
     4381 cgtgcgctct cgttccagca gcgcatgctt tcgccaaaga ccgccgccgt acccggtaag
     4441 gtcgccatcg cttccaatca cgcgatggca tggaatcaca atcgcgacgc ggttgtctcc
     4501 gttggcgcgc gctacagccc gcatccctgt cggcattccg atctgacgcg ccaggtcgcc
     4561 gtaggagcgc gtttcgccgt acgggatcgt gcgcagcgcc gcccacacct tctcttgaaa
     4621 cggcgtgccg cgcacgtcga gtggcgccgt gaattcgcgc acctcgcccg cgaagtagtc
     4681 tgccagttgc ttgcgcatct cgcgcgtgtg ctcgttctcg cccggtacca ccgaatcgcc
     4741 gcaccacttc cgcacgcgcc ccagttgcgt ttcgagcagc ctccggtcgg tgtattcgag
     4801 aatgcagatg ccgcgctccg tggcgcccgc gatcatcggc ccaatcggcg tctcgaccca
     4861 cgaaatcttc accgtgctcg cgtccgccgc tttcccaggc gtgtagccga acacccgctc
     4921 aaacgccgaa cgaaacccgc tatgcgattc gaagccgctc tcaaacaccg cgtcgtcgat
     4981 cgaagcgcca tcctgaattt ccgtgaatgc ttctcccaac cgccgcgctc tcatataggc
     5041 ttgaaaggtc atgccgtact tcgaatgaaa gtatcggcgc acgcgcaccg gttccattcc
     5101 catctcacgc aggtcctcat cacggaagcg ccgcttcgga tctttctcaa tctcgtcgag
     5161 cagcggtcgc agccactccg gatcgcttcc cggggccgtc atcggactgc agcgcttgca
     5221 tggcctgtat ccggccttga gcgcctcgcg cgccgagtcg aagaattcaa tatttgccgc
     5281 cagcggcttg cgcgcgctgc acgacggaat acaaaagatg ccggtagtgc gtacgcccgt
     5341 atagaacagg ccgtcgaacg agctgtcggc ccgttgaaac gcatccacca tctgctctct
     5401 ggtagcaaac aggctcatgt agacattgta gaaagccccg cgcccgcgct ccaccgagaa
     5461 ctgaacaccg aattccgaat cttcccagcg taaaaccagc gtggagcgcg cgttttcggt
     5521 tatattgccc tcttggagag actctcagca gttctcgaac agtttcaccg cgctgtcgta
     5581 tgctgcgcgg gcgatgtcat gctcgatcgc ttcatttatg gcagcgtctc gcgcatttcg
     5641 cccgaagctc cggtgccggt tctgcaaatt cgcgaacagc actccatgct cggaggcgcc
     5701 ggcaacgccg ttcgcaactt gcacgcgctc ggctgctccg ttcgcttcac cagcgtcgtg
     5761 ggcgaggatg ccgccgccgc cgaaatcgaa agcgcgctcg ttgcgatgcc gaagtgccaa
     5821 tggttcctcg catacgaact cggccggcag accatcgtca agactcgcta catcgccgat
     5881 tcgcagcagg tgatgcgcgc cgacgccgag tcgaagcatg agatcgctgc cgagcctttc
     5941 gccgcggcct tggaacggtt ccaagcaaca ctcgaaggcg catctgtcgt cttgctctcc
     6001 gattacggta aggggctgct cacagccggc cgcgcccacg catttatcga tttggcgcgc
     6061 gcccgcggca tcccgtcgat tgtcgatccg aagggcgccg ctttccgccg ctacaacggg
     6121 gccacggtca tcaagcccaa tctcaaagag ctcgccgaag ctacgggaat gccggtcgat
     6181 acagaccagg cgcgcgaagc cgcggcgcgc gcgctgctgg ccgatcttga catcgaacac
     6241 ctgcttgtga cctgcggtgc ttc
//
LOCUS       DASZMC010000393         5702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4014476,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000393 DASZMC010000000
VERSION     DASZMC010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4014476"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VG274_08065"
     CDS             <1..610
                     /locus_tag="VG274_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06647.1"
                     /translation="HTVDPKFHVSNCEALQQMKRKSRFERYVVAVRPDGTFRLNLIDG
                     KRKTEELRKLAVCQNCMDKLRFDGFVMGLPRPDRIRLVQSFTLDRFFEKFSKTLLTDI
                     PQFNSDEAPLNDYAQGFSTKSNFVRAAANWTCQNPKCRAWLGGAHHRRFLHVHHVNGM
                     KCDDESQNHRVLCIACHAEQPSHGHMKRLPEYKQFLAIRATL"
     gene            complement(607..837)
                     /locus_tag="VG274_08070"
     CDS             complement(607..837)
                     /locus_tag="VG274_08070"
                     /inference="COORDINATES: protein motif:HMM:NF042870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HWE06648.1"
                     /translation="MLNTNEVRARAAEFARDWKDARYERGEAQSFYNDFFNVFGMKRR
                     QVATFEEPVKRLGDKRGFLDLFWKSTLLAEPR"
     gene            1113..1409
                     /locus_tag="VG274_08075"
     CDS             1113..1409
                     /locus_tag="VG274_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06649.1"
                     /translation="MQSATPVLDLSAIAVNAARLRMAPGISFAKLPSAAPAAGISLPS
                     ISFGSVRAEIGGTAGGLKHGANFRLEGVSLFGSSISGSVDGRSARLTFTWPANL"
     gene            1509..1694
                     /locus_tag="VG274_08080"
     CDS             1509..1694
                     /locus_tag="VG274_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06650.1"
                     /translation="MDRAEQYRGHAEELRSIARDWISDESQAALLKVALDYERMAKAL
                     ERFSDAREIDARLSIAS"
     gene            complement(1739..1948)
                     /locus_tag="VG274_08085"
     CDS             complement(1739..1948)
                     /locus_tag="VG274_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06651.1"
                     /translation="MPGYRLCFLNKDNRIMTHIEHVCDDDLSALAKARTLATSFAVEI
                     WQEERRVAIVEKGTAPRLDNDLRLL"
     gene            2394..2786
                     /locus_tag="VG274_08090"
     CDS             2394..2786
                     /locus_tag="VG274_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWE06652.1"
                     /translation="MFHADDVVARILAVARRSRRQPQSVITAGKLTVNVEAKTVEAGG
                     SRRVHLTRKEYQMLELLALRKGTTLSKEMFMDHLYGATNQPEPKIIDVFICKLRKKLA
                     AATSSDDYIETVWGHGYSLRDPPSGEAA"
     gene            complement(2830..3114)
                     /locus_tag="VG274_08095"
     CDS             complement(2830..3114)
                     /locus_tag="VG274_08095"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HWE06653.1"
                     /translation="MPALTFNLHGAIREQRSMGETGMARYSRSSGKDVERAVKRRRKG
                     KLKRGKGGRGGTVKSRKQAIAIGLSEARKKGKKLPKKAKSKRRKSRKSKT"
     gene            3220..4260
                     /locus_tag="VG274_08100"
     CDS             3220..4260
                     /locus_tag="VG274_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06654.1"
                     /translation="MKPVSAATLAKKLGLRIAKPASLTIRRKPDGESWTYWSGRGRRI
                     SDQRVVMRLNKLAMPPAYEDVRLAADPRAHIQAIGRDAAGRLQYRYHRDWEKVRDIRK
                     AQQLIDLVEVLPRIRRALAAKLGEDILSREFALAAIVELIAASAIRAGSEEYAREHKT
                     RGAATLLKANVRCGEDGTILLCFRAKGGRQVRKAVKHARLSAAIGRLRELPGMRLFQF
                     RGEDGAVRAASAADANAYLREIAGCPISLKDFRTLCGTADVIETLAAIEPAKNARLRR
                     KQIVEAIKSAAKTLENTPAVCRKSYVHSAVLTAFEAGMLKEFATVLKAARSSTPAQNA
                     VGAVLEASAAAA"
     gene            4487..5617
                     /gene="dinB"
                     /locus_tag="VG274_08105"
     CDS             4487..5617
                     /gene="dinB"
                     /locus_tag="VG274_08105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWE06655.1"
                     /translation="MAGRALPGVGLRKTDSAMVVAEIRKIIHVDMDAFYASVEQRDDP
                     ALRGRPVAVGHGAKRGVVAAASYEARAFGVRSAMPSTTAMRQCPELVFVRPRFDVYRV
                     VSQQIREIFADYTALVEPLSLDEAYLDVSENLRGLPSASATASEIRARIFSATRLTAS
                     AGISYNKFLAKLASEHRKPNGQFVVTPAMGLSFIAALPVRKFHGVGPVTADRMNRLGI
                     FTGADLARQSLSLLKQNFGKAGPWYYSIARGEDDRAVTPHRLRKSSGSETTFSDDLTD
                     PAAIEAGVAAMADEVWAWCEKAQSFGRTVTVKIKYADFRQATRSRTLRTHVASKDALR
                     DVSIGLVRTVFPPATGIRLLGVTMSGFEWNEAPASVQLGFDL"
BASE COUNT         1084 a         1788 c         1753 g         1077 t
ORIGIN      
        1 ccatacggtc gatccgaaat ttcacgtttc aaattgtgag gctttgcagc agatgaagcg
       61 aaaatcccga tttgaaagat acgtggttgc agtgaggccc gatgggacct ttcgattgaa
      121 tcttattgat ggaaaacgca agacagagga attacgaaaa ttggcggtgt gtcagaattg
      181 catggataaa ttgcggttcg atggtttcgt gatggggctc ccaagaccag atcgaatcag
      241 gctcgtgcaa agcttcacgc tggacaggtt ctttgagaaa ttttcgaaga cgctactgac
      301 cgatatcccg cagttcaact ccgatgaggc gccgctaaac gactacgctc aaggcttctc
      361 taccaagagc aacttcgttc gagctgccgc taattggaca tgccagaatc caaaatgtcg
      421 ggcatggttg ggaggcgccc accatcggag atttctgcac gtacatcacg tgaacggcat
      481 gaaatgcgac gacgaatcac aaaatcatcg cgttctctgc attgcctgcc acgccgaaca
      541 gcctagccat gggcacatga aacgacttcc ggaatacaag cagttcttag cgatacgagc
      601 gactctctag cgcggttcgg cgagcagcgt gcttttccag aacaggtcca gaaatccgcg
      661 tttgtcgccg agccgcttca ccggctcttc gaaggtcgcc acctggcggc gcttcatgcc
      721 gaagacgttg aagaagtcgt tgtagaagga ctgcgcttcg ccccgctcgt aacgggcgtc
      781 cttccagtcc cgggcaaact ccgccgcccg cgcccgcact tcgttggtgt taagcaagca
      841 gcccggcccc ggtcgcagaa tcgtaggccc agcaatagca cattttcgca ccagcggagg
      901 cgcccggcgc cgcgcgcacg caacttgaac ccgccgacag cgtttaagcg gcaatagggg
      961 gagcgtgtgc cacgcgtgtt gcgccgcttg ttgccgttgc atttgtgtgt actgcgccgg
     1021 tcgcgcaatc gcagattgtc gcggtcccgc cggtttcgcc aggcgcgcct tccttcagca
     1081 gtccggaacc gctcaggcat gacggacgcc ccatgcaaag cgcaactccc gtgctcgacc
     1141 tgtccgcgat cgcggtcaat gcggcgcgcc tccgcatggc gcccggcata tcgttcgcga
     1201 aactgccgtc tgccgcgccg gccgccggaa tttcgcttcc atcgatttca ttcggatcgg
     1261 ttcgcgcaga aatcggcggg accgcgggcg ggctcaagca cggcgcgaat ttccggctgg
     1321 aaggcgtaag tctctttggc agcagcattt cgggcagcgt cgacggacgc tccgcgcgtc
     1381 tcaccttcac ctggccggcc aacctctagg cacgccggat taaccatcga aggatgtggg
     1441 ctgttgccga tccgcacctt tcctcccaat cccgcgttgt agtatgatcg cgaatcaggg
     1501 gtatgggcat ggacagagcc gaacagtacc gcggtcacgc ggaagagttg cgttcgatcg
     1561 cccgcgactg gatcagcgac gaatcccagg ctgcgcttct caaggtcgcg ctcgattacg
     1621 agcggatggc gaaggcgctg gagcgcttca gcgacgcgcg agagattgac gcccgtctga
     1681 gcatcgcgtc gtagccgatc gtcgcacggc ttcgcgaaag taatgagccg gtggcgggct
     1741 agagcaatct caaatcattg tccaggcgag gggcggtgcc cttttccaca atcgcaacgc
     1801 gacgctcttc ctgccaaatc tcaactgcga aactcgtggc gagggtgcgg gctttcgcca
     1861 acgcgctcag atcatcgtcg cagacatgtt cgatatgcgt catgatgcga ttgtctttgt
     1921 tcagaaaaca cagccgataa cccggcatgc ctgcctcctc gatcccgcca ggacgggaat
     1981 gttgtatcgt aggatatttc ctgacggtcg cgcgatcgcg gcgccactcg ccataccggc
     2041 gatttcccca ccgtacagcc ggcttgaatc gaggactgtg cagttttgac cacctcgggc
     2101 gcggggcaaa aaaattgagg accgtcgctg ccgcgcacgc cattgatcgc cgcgaatttc
     2161 ggctccccgg gcgaacaacc gcgtgaatcg cagttccgcg ccgaaaacgc ggcttcgctc
     2221 cgccggtgag caattctgct cagctttggt ttttggcccc gtctagcctt cttctccccg
     2281 tggagaggct ccccgcgaac atcgaggggc atctcgggcg ttcgcatgcc gccatgtccc
     2341 cgtagatggc tcgcggcagc tcagattcat atgacactgg aggaaatagc cggatgttcc
     2401 acgcagacga tgtggtcgcg cgcattctgg ccgttgcgcg ccgctccaga aggcagccgc
     2461 aatccgtcat cacggcgggc aagcttaccg tcaatgtcga ggcaaagacg gtcgaggccg
     2521 gcggcagccg ccgcgtgcat ctgaccagga aggaatatca gatgctggag ctgcttgctt
     2581 tgcgaaaagg gaccacgctg tccaaggaga tgttcatgga ccatctctat ggcgccacga
     2641 accagccgga gcccaagatc atcgacgtct tcatctgcaa gttgagaaag aagcttgcag
     2701 cggccaccag cagtgatgat tacatcgaga ccgtgtgggg ccacggctat tcgttgcgcg
     2761 atcccccgag cggagaagcc gcgtaaacgc gggatcattg ccggcgttgc gccggttggg
     2821 ctttgtcggt cacgtcttcg acttgcgcga tttccggcgc ttcgattttg cttttttcgg
     2881 aagcttcttg cccttcttgc gggcttccga caggccgatg gcgatcgcct gcttgcggct
     2941 cttgaccgtg ccgccgcggc cgcccttgcc gcgtttgagc ttgcccttcc tgcgccgttt
     3001 cacggcgcgt tccacgtctt tgccggaact gcgcgaatat ctggccatgc ccgtttctcc
     3061 catcgaacgc tgctcgcgaa tcgccccatg caggttgaac gttaatgccg gcatggatat
     3121 tcggttgcac gaacgtcgct tttcgcgccg gcggcagctg ccgcggccgc gcaagtatga
     3181 gggcggcaac cgaacgcgaa ttggggacgt tggtggggaa tgaagcccgt ttctgccgcc
     3241 acgctcgcga agaagctcgg cctgcgcatc gcgaagcccg cttcgctcac cattcggcgc
     3301 aagccggatg gcgagagctg gacctactgg tcgggccgcg ggcggcgcat ttccgaccag
     3361 cgtgtcgtta tgcggctcaa caaactcgcc atgccgccgg cctatgagga cgtgcgcctg
     3421 gctgccgatc ccagagccca tatccaggcc atcggccgcg acgcggcagg acgcctgcaa
     3481 taccgctatc atcgggattg ggaaaaagtc cgcgacatcc gcaaggcgca gcagctgatc
     3541 gaccttgtcg aggtgcttcc ccgcatccgc cgcgcgctcg ccgcgaaact gggcgaggac
     3601 atcctgtccc gggaattcgc gcttgcggcg atcgtcgaac tcatcgccgc ttccgccatc
     3661 cgcgccggca gcgaagaata tgcgcgcgaa cacaagacgc gcggggccgc cacgcttctc
     3721 aaggcgaatg tgcgctgcgg cgaggacggc acgatcctcc tgtgcttccg cgccaagggc
     3781 ggcaggcagg tccgcaaggc ggtgaaacac gcgagattaa gtgcggcgat cgggcgtttg
     3841 cgcgaactgc cgggcatgcg cttgttccag ttccgcggcg aggatggagc ggtccgcgcc
     3901 gccagtgccg ccgatgcgaa tgcgtatctc cgagaaatcg caggctgccc aatctcgctc
     3961 aaggatttcc gcacgctgtg cggcactgcg gacgtcatcg agacgctggc ggcgatcgag
     4021 ccggcgaaga acgcccgcct gcgcaggaaa cagatcgtcg aagcgatcaa atccgcggcc
     4081 aaaacgctcg agaatacgcc cgccgtgtgc cgcaaaagct acgtgcattc cgccgtgctg
     4141 accgcattcg aagccggaat gctgaaggaa ttcgctaccg tcctgaaagc cgcgcgctct
     4201 tccactccgg cgcagaacgc cgtcggcgcc gtgctcgaag cctccgccgc cgctgcatag
     4261 cgaaccgccg agccgccaaa gatgccaaca agactgggca cgcggagccg cggagagcgc
     4321 ggagtgagaa cgtgcagcgc agagacacag aggacacgga gaaaacggat cgcgcgggat
     4381 ggtcaggttt aacccgacca cgtatgcgcg caaaatcttc gtccgcgtcc tccgcgtctc
     4441 cgcgtgagat atttttcttg cgccttggcg gcttggcagt tcgattttgg ccggcagggc
     4501 gctgcccggt gtaggattgc gcaaaaccga ttccgcgatg gtcgtggccg aaatccgcaa
     4561 gatcatccat gtcgacatgg acgccttcta tgcgtcggtg gagcagcgcg acgatcccgc
     4621 gttgcgcggg cgtcccgtcg ccgtcgggca cggcgcgaaa cgcggcgtcg tcgccgcggc
     4681 gagctacgag gcccgcgcct tcggcgtgcg ttcggcgatg ccctcgacga cggcgatgcg
     4741 ccaatgtccg gagctggtgt tcgtgcggcc gcgcttcgat gtctatcgtg tcgtgtcgca
     4801 gcagattcgc gagatatttg cggactacac cgccctcgtc gagccgctgt cgctcgacga
     4861 agcctatctc gacgtgtcgg agaatcttcg cggcctgccg tccgcatcgg caaccgcgag
     4921 cgaaatccgc gcgcggattt tctccgcgac ccgcctcacc gcctcggccg gcatctccta
     4981 caacaagttc ctggcgaagc tcgcctccga acatcgaaag cccaatgggc aattcgtggt
     5041 gacgccggcg atggggcttt ccttcatcgc cgcgctgccg gtgcggaaat tccacggcgt
     5101 ggggccggtc accgccgaca ggatgaaccg gctcggcatt ttcaccggcg cggacctcgc
     5161 gcggcagtcg ctcagcctgc tcaagcagaa tttcggcaag gccgggccct ggtactattc
     5221 gatcgcccgc ggcgaagacg atcgggcggt gacgccgcac cgcctgcgca aatcgtcggg
     5281 ctccgaaacg acattctcgg acgacctgac cgatcccgcc gccatcgaag cgggcgtcgc
     5341 ggcgatggcg gacgaagtgt gggcgtggtg cgagaaggcg caaagtttcg gccgcaccgt
     5401 gacggtgaag atcaaatacg cggatttccg tcaggcgacg cgcagccgga ccttgcgtac
     5461 ccatgtcgca tcgaaagatg ccttgcgcga cgtcagcatc ggcctggtgc gcacggtatt
     5521 cccgcccgcg accggcatcc gcctgctcgg cgtgacgatg tcggggttcg aatggaacga
     5581 agcgcccgca tcggtccagc tgggtttcga cctgtaaacg gcgcaactgc tggcgcaccg
     5641 gacaggatga aaatgggaac cggttaatcg tcggacttcg tcggaatgga ggaccttatc
     5701 at
//
LOCUS       DASZMC010000394         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_979073,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000394 DASZMC010000000
VERSION     DASZMC010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979073"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VG274_08110"
     CDS             complement(<1..378)
                     /locus_tag="VG274_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWE06656.1"
                     /translation="MADPIPKKQANSIDAQVGNRVRLRRMLVGMSQEKLGEMLGLTFQ
                     QVQKYEKGVNRIGAGRLYRVAQILGVSVSYFYEDVVEQGPTASPVANDRATPSAVEFL
                     SSSEGMQLTLAFMRIREPKVRRRV"
     gene            complement(414..>1608)
                     /gene="lnt"
                     /locus_tag="VG274_08115"
     CDS             complement(414..>1608)
                     /gene="lnt"
                     /locus_tag="VG274_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWE06657.1"
                     /translation="WRPGTSRIFLLGAAYAICEWLRGHLLTGFPWNLAAYAWGAAPGV
                     MQCAAAIGAYGLTLLTLLLGFSLAEFASARRHAWRLPSALIACFALLWIGGVLRMQFV
                     NPGFVPGVHLRLVQPNVPQAEKYVARFRARNWARLVDLSQTPSRMMPTHIIWPEAAPP
                     FLLARSPEALDQIASLTPDDRVLMTGAVRTLISPEGGFHATNSFYIFSHAGRLLGVYD
                     KFHLVPFGEFLPLPHLLHALGITKLVDMPDGFDHGPGPLTFDVPGAPPMGPLICYEVA
                     FPAAVVGTVRPGWLVNVTDDSWFGPSSGPFQHLLMARMRAIEEGIPIARAANTGVSAV
                     IDPLGRVVASLGLGKAGIVDAPLPSALPSTPYARLGDVGFALLLAGGLLWAFTRPGSV
                     AKKMA"
BASE COUNT          329 a          511 c          515 g          253 t
ORIGIN      
        1 cacccgcctg cggaccttcg gctcccggat ccgcatgaag gcaagagtca gttgcatccc
       61 ttcgctgctg gagaggaatt cgacggccga gggagtcgcg cgatcattgg cgaccggcga
      121 ggccgtcgga ccctgctcca ccacgtcctc atagaaatag ctcacggaaa cgccgaggat
      181 ctgcgccacg cgataaaggc gcccggcgcc gatccggttc actcccttct cgtatttctg
      241 cacctgctgg aaggtgaggc ccagcatttc gccgagcttc tcctggctca tcccgacaag
      301 catgcgccgc agccgcaccc ggttgccgac ctgggcgtcg atcgagttcg cctgcttttt
      361 cggtatcggg tctgccacga attttccgtt ttctttctac agcttagcgg gaattaagcc
      421 attttcttgg cgacgctccc tggccgcgtg aacgcccaca aaaggccgcc cgcgagcagc
      481 agggcgaatc ccacgtcccc taaacgcgca tagggcgtgg atggcagcgc actcggcagg
      541 ggggcatcga cgattcccgc ctttcccagg cccaagctgg caacaacgcg ccctaatgga
      601 tcgatcactg ccgaaacgcc ggtattggcg gcccgcgcaa tcgggatgcc ttcctcgatg
      661 gcacgcatgc gggccatcag aaggtgctgg aatgggccgc tggaagggcc aaaccaggaa
      721 tcatcggtca cgttcacgag ccagccggga cgcacggttc caaccacggc ggccgggaac
      781 gccacttcgt agcagatcag cggtcccatg ggcggcgcgc ccggcacatc gaacgtcagc
      841 ggcccagggc catgatcgaa accgtcaggc atgtcgacga gcttggtgat gccgagcgcg
      901 tgcagcaggt gtggaagagg aagaaattcg ccgaacggca caaggtggaa cttgtcgtaa
      961 acgccaagca ggcggcctgc gtgcgagaag atgtaaaaac tgttggtggc gtggaacccc
     1021 ccttcgggag agatgagggt gcgcaccgct cccgtcatca ggacgcgatc gtccggcgtg
     1081 agagacgcga tctgatcgag agcctccggc gagcgcgcga ggagaaaagg cggcgccgcc
     1141 tctggccaga tgatgtgcgt cggcatcatg cgcgacggcg tctggctcag atcgacgagc
     1201 cgcgcccagt tccgggcccg gaatcgagca acatacttct ccgcttgcgg aacattcggc
     1261 tggacgagtc taagatgaac gccgggcacg aatcccggat tgacgaactg cattcgcagt
     1321 acgccgccga tccacaggag cgcgaagcat gcaatcagcg ccgaaggcag gcgccatgcg
     1381 tgtcgccggg cgctcgcgaa ttccgcgagt gagaagccca gcagaagcgt cagcaaggtg
     1441 agcccgtaag cgccgatcgc ggccgcgcat tgcatgacgc cgggcgcggc gccccacgca
     1501 taggcagcca gattccacgg aaacccggtc agaagatggc cccgcagcca ttcgcaaatt
     1561 gcgtaggccg cgccgaggag aaaaatgcgt gaggtgcccg ggcgccag
//
LOCUS       DASZMC010000395         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3192259,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000395 DASZMC010000000
VERSION     DASZMC010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3192259"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VG274_08120"
     CDS             complement(<1..317)
                     /locus_tag="VG274_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HWE06658.1"
                     /translation="MKLVLCGTPEFAVPTLEALLEQGHEIALVLTQPDRPAGRKMELQ
                     QSAVKRTAVARGLRVLQPEKIKNNVELRAELESIEPDAIVVVAYGRIIPEWMLKLPRF
                     ANI"
     gene            complement(324..899)
                     /gene="def"
                     /locus_tag="VG274_08125"
     CDS             complement(324..899)
                     /gene="def"
                     /locus_tag="VG274_08125"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWE06659.1"
                     /translation="MSKKEKEQQTSAATGAADASTAKIHAIVKYPDPVLSARGENVTE
                     FGPELGQFVEEMWASMYAAHGIGLAAPQVGVSKRITVIDASFKERPDEKLVLINPEII
                     KREGKQFEEEGCLSLPEIRDKVHRAARVTVRAQDVNGEFFEADGEELLARAIQHEIDH
                     LDGILFIDRLSPLKRDLAKRKIRKLQKNGEW"
     gene            complement(903..>2042)
                     /gene="aroC"
                     /locus_tag="VG274_08130"
     CDS             complement(903..>2042)
                     /gene="aroC"
                     /locus_tag="VG274_08130"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWE06660.1"
                     /translation="VALISGLPAGIPVDQEFVNRELWRRQQGYGRGGRMRIEKDEAHI
                     LSGVRHGKTIGSPIAMTLANRDWKNWEQILPVEAGDASLHKSVASPRPGHADLAGALK
                     YDFKDARYVLERASARESAARVACGALAKLLLRELGVQVASHVVRVGTAQLAREVSFD
                     EVAAIAQREDVMLACADADSEAAMKAEVDKALRTGDTVGGVFEVIVHGLPPGIGTHVN
                     WDERLDGLLAQALMSLQAVKAVELGRGVTAAESAGSVVHDAIGYAGEGEGGEGFTRFR
                     REKNNAGGVEGGMSNGEDVVVRGYLKPISTLRRPLGSVSFETREATKASYERSDVCVV
                     PAAGVAGEAMVALTIAKLVLEKFGGDSLGELKRNFEGYREQIRAF"
BASE COUNT          365 a          767 c          525 g          385 t
ORIGIN      
        1 ttgatattgg cgaagcgcgg cagcttcagc atccactccg gaataatgcg gccatacgcg
       61 acaacgacga tcgcgtccgg ctcaatcgat tcgagctccg cacgcagctc cacgttgttc
      121 ttgatcttct ccggctgcag cacgcgcagc ccgcgtgcaa ccgccgtgcg ctttaccgca
      181 gactgctgca gttccatctt gcggccggca gggcggtccg gctgcgtcag caccagcgca
      241 atctcgtgcc cctgctcaag cagcgcctcc agcgtaggca cagcgaactc cggcgttccg
      301 cacagaacca gcttcatgcc acatcaccac tcgccgttct tttgcagctt gcgaatcttg
      361 cgcttcgcca ggtcgcgctt cagcgggctc agccggtcga taaacaggat gccgtcgaga
      421 tggtcgatct catgctgaat cgcgcgcgcc agcaactcct caccgtcggc ctcgaagaac
      481 tcgccgttca catcctgggc acgcaccgtg acgcgggccg cgcgatgcac cttgtcgcga
      541 atttcaggaa ggctcaggca gccctcctct tcaaactgct tgccttcgcg cttgatgatc
      601 tccggattga tcagcacaag cttctcgtct ggccgttcct tgaagctggc atcgatgacc
      661 gtgatgcgct tcgacacgcc aacctgcggc gcggccagcc cgatcccatg ggccgcgtac
      721 atgctggccc acatctcttc gacaaactgg ccgagctccg ggccgaactc cgtgacattc
      781 tcgccccgcg ccgacagcac cggatcggga tacttcacaa tcgcgtggat cttcgctgtg
      841 ctcgcgtctg ccgcgccggt ggccgcactt gtctgctgct ctttttcttt cttgctcatc
      901 tctcaaaagg ctcgaatctg ttcccgatat ccctcaaaat tgcgcttcag ttcgccaaga
      961 ctgtcgccgc caaacttctc caaaactaat ttcgcgatcg tcagcgcgac catggcctcg
     1021 cctgcaacac cggcggccgg caccacgcaa acgtccgacc gctcatacga tgccttggtc
     1081 gcctcacgcg tttcaaatga gacagaaccg agcgggcgcc gcagcgtcga aatcggcttc
     1141 aggtatccgc gcaccacaac gtcctcaccg ttcgacatgc cgccttcaac gccgccggca
     1201 ttattcttct cacgccggaa tcgcgtgaac ccctcgccac cttcaccttc gccggcgtag
     1261 ccgatggcgt cgtgcaccac ggaaccagcc gactcagccg ccgttacacc gcggccaagc
     1321 tccaccgcct tcacagcctg caagctcatc agcgcctgtg cgagcaggcc atccagccgc
     1381 tcgtcccaat tcacgtgcgt tccaatgccc ggcggcagcc cgtgcacgat cacctcaaac
     1441 acgccgccca ccgtatcgcc ggtgcgcagc gccttgtcga cctccgcctt catcgccgct
     1501 tcggagtccg catcggcgca cgccagcatc acgtcctcgc gctgcgcaat cgccgcaacc
     1561 tcgtcgaagc tcacctcgcg cgcaagctgc gctgtgccca cccgcaccac gtgactcgca
     1621 acctgcacgc ccaactcgcg caacagcagc ttcgccagcg cgccacaggc cacacgcgcc
     1681 gcagactcgc gagcactcgc acgctccaac acgtaacgcg cgtccttgaa gtcatatttc
     1741 agcgcgccgg caaggtccgc gtgccccggt cttgggctcg cgaccgattt gtgcagcgat
     1801 gcatcgccgg cttccaccgg cagaatctgc tcccagttct tccagtcgcg attcgcgagc
     1861 gtcatcgcaa tcggcgaacc gatcgtcttg ccgtgccgca cgccagagag aatatgcgcc
     1921 tcatccttct cgatgcgcat gcggccgccg cggccatagc cctgctggcg ccgccacagc
     1981 tcgcggttca caaactcctg gtcgacagga attccagcgg gcagcccgct gatcagtgca
     2041 ac
//
LOCUS       DASZMC010000396         6527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1296346,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000396 DASZMC010000000
VERSION     DASZMC010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296346"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VG274_08135"
     CDS             <1..1939
                     /locus_tag="VG274_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWE06661.1"
                     /translation="AETGNKPILLSIGYTACHWCHVMNEESFRDAETAALMNELFVNI
                     KVDREERPDIDQIYQAAANSLGHTGGWPLTVFLTPSGDPFYAGAYFPKEDRYGQPAFK
                     RVLPEVARLFHEQPDPVANTAARVQQAFANLWARDLRGNLDGAVLDQCAIRIGQRFDI
                     FYGGLTGAPKFPSTGHVELLWRAHLRTGLGQFLQLALTTLDHMALGGMYDHVGGGFAR
                     YSTDERWQVPHFEKMLYDNALLIDLYTMAWQHNRAAAYRERIEDTAAWLLREMGVEQA
                     FASSLDADQDGEEGKYYLWSEAEIDAALAGTFAQRFKEAYGVRREGNFQGRNLLQRIG
                     GVYPLPEADEILLKKQRELLLAARLKRTPPLRDDKVLADWNGMAIAALANAGMVFRKA
                     QWTAAAIRAFAFIESALGDGDRLYHSWRNGTRHHTGFSDDYAHMARAAFTLWEATGDK
                     RYLGRAQAWVHVLNEHFWDSQNGGYFYTSDDSDPLIVRTRIVFDQAAPCANGLLVGLL
                     SRLHLATADNLYRDRANALIQAFASEVSRAFLSMSSYINGLEVAMTALQIVVVGQIDN
                     SRTHELVNAALGRSLPNRTLIVVDPRDTLPAGHPAQGKTMQNGQPTAYVCQRMSCSAP
                     ITNPVTLSQVLLLPARPQGQA"
     gene            1974..2672
                     /locus_tag="VG274_08140"
     CDS             1974..2672
                     /locus_tag="VG274_08140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWE06662.1"
                     /translation="MTGKDITIRTADGSFGGYLASPSAGRGAGIVVIQEIFGVNGFVR
                     AVADGFAAHGYFALAPDLFWRIEPGVQLSDKTDAEWSRAFELMGKFNADLGVKDIQAA
                     IAELRNTPGCSGKVGAVGYCLGGLLAYLTAARTDADASVAYYGVNIQKMLGEAGSIRK
                     PLMLHIAGKDEYVPSAAQKAIVDGLKTCPHVTIHLYPEMDHAFARTGGAHYDRANAEL
                     ANGRTSTFLRQHLS"
     gene            2675..3646
                     /locus_tag="VG274_08145"
     CDS             2675..3646
                     /locus_tag="VG274_08145"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWE06663.1"
                     /translation="MKAIRFARTGGPEVLALEDIDLPAPRAGEVRVRHTAIGVNFIDT
                     YHRCGLYPVSLPSGIGLEGAGVVEEVGDGVTAFRKGDHAAYCTGPLGAYAEANNVPAD
                     RLVKIPSGIAGETAAASLLKGMTAQYLLKRTYSVQHGQTILFHAAAGGVGSIACQWAK
                     HLGATVIGTVGSDEKVALAKANGCAHVLNTGSDAWSRRVREITGGAGVPVVYDSVGKD
                     TFAGSLDCLAVRGMMVSFGNSSGAVPAFEPGTLSAKGSLYLTRPMLFHYTRSAKELQE
                     TAEDLFAVLLSGAVKIAVHQRFPLAEARRAHEALHSRATTGATVLIP"
     gene            complement(3677..4933)
                     /gene="rho"
                     /locus_tag="VG274_08150"
     CDS             complement(3677..4933)
                     /gene="rho"
                     /locus_tag="VG274_08150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWE06664.1"
                     /translation="MKLQDLKTKSPTDLLAFAEDLEIENASAMRKQDMLFAILKELAD
                     RNVEITGSGVLETLQDGFGFLRSPESNYLAGPDDIYVSPSQIRRFGLRTGDTVDGPIR
                     SPKDGERYFALLKVSTINFEDPEKIKHKVHFDNLTPLYPTKWLNLEIEDPTIKDKTGR
                     IIDIVAPQGKGQRALITAQPRTGKTVILQNIARAIAVNHPECYLIVLLIDERPEEVTD
                     MQRSVKGEVVSSTFDEPATRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEE
                     FKGTGNSEIILDRKVADKRVFPAIDILRSGTRKDELLIDKGTLAKTYVLRRILAPMGT
                     IDAIEFLLDKMRSSKTNSDFFESMNT"
     gene            complement(5186..5641)
                     /gene="hemJ"
                     /locus_tag="VG274_08155"
     CDS             complement(5186..5641)
                     /gene="hemJ"
                     /locus_tag="VG274_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HWE06665.1"
                     /translation="MTAAIESTEFYLWIKALHVIAVIAWMAGMLYLPRLFVYHCETLP
                     GSPDSERFKRMERRLLRAIVNPAMIAVWILGLTLSFLPATDAWRQNWFHLKFALVVVM
                     SGVHGLNARWVRAFASDQNAHSARFYRIWNEVPAALLVFIVILAVVKPF"
     gene            complement(5638..>6527)
                     /gene="hemH"
                     /locus_tag="VG274_08160"
     CDS             complement(5638..>6527)
                     /gene="hemH"
                     /locus_tag="VG274_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWE06666.1"
                     /translation="RAPVARAIYERIGGKSPLLEQTIAQAGALEQELVRRGAEARVFV
                     AMRAWDPMSDETAAAVAGFAPDCVVLLPLYPQFSTTTTASSLSAWCAAAAKAGLSAEH
                     KRVCCYPWNEGFIRGLAERVRISLLDRRPELSYRLLFSAHGLPQRIVDRGDPYRWQIE
                     RTVGALVHELGEDALDWRISYQSRVGPLKWIGPATDEEIRRAGGEGKGLVVVPVAFVS
                     EHSETLVELDMDYAALAHKAGVPDYLRAATVGTNEAFIHGLGDLVLHAVASAGPVTCG
                     AARICPPDLALCGMREARQ"
BASE COUNT         1186 a         2120 c         2035 g         1186 t
ORIGIN      
        1 ggcggaaacg ggcaacaagc cgatcctgct ctccatcggc tacacggcct gccactggtg
       61 ccacgtgatg aacgaggaga gcttccggga cgcggagacc gcggcgctga tgaacgagct
      121 gttcgtcaac atcaaggtcg accgcgagga gcggcccgac atcgaccaga tctaccaggc
      181 ggccgccaac tcgctgggac atacgggcgg ctggccgctg actgtcttcc tcacgccgtc
      241 gggcgaccct ttctatgccg gcgcctattt ccccaaggag gaccgctacg gccagccggc
      301 gttcaagcgc gtcctgccgg aagtggcgcg cctgttccac gagcagcccg atccggtcgc
      361 caacaccgcg gcgcgcgtgc agcaggcctt cgccaatctg tgggcgcgcg atctgcgcgg
      421 caatctcgac ggagcggtcc tcgatcagtg cgcgatccgc atcggccagc gcttcgatat
      481 tttctacggc ggtctcaccg gcgcgccgaa atttccctcg accgggcacg tggaactgct
      541 gtggcgcgcc catctgcgca ccggcctcgg ccagtttctg cagctcgcgc tcaccacgct
      601 cgaccacatg gcgctcggcg gcatgtacga ccacgtcggc ggcggcttcg cccgctactc
      661 gaccgacgaa cgctggcagg tgccgcattt cgagaagatg ctctacgaca acgcgctgct
      721 catcgacctc tacacgatgg cgtggcagca caatcgcgcc gccgcctatc gcgagcgcat
      781 cgaagatacc gcggcctggc ttctgcgcga gatgggcgtc gagcaggcct tcgcgtcgag
      841 cctcgacgcc gaccaggatg gcgaagaggg aaaatactat ctgtggagcg aggccgagat
      901 cgacgccgcc ctggcgggca cgttcgcgca gcgcttcaag gaagcctacg gcgtccgccg
      961 cgagggcaat ttccagggac gcaatctgct tcagcgcatc ggtggcgtct atccgctgcc
     1021 cgaggccgat gagatcctgc tcaagaaaca gcgcgagctg ctgctcgccg cgcggctgaa
     1081 gcgaacgccg ccgctccgcg acgacaaggt actcgccgac tggaacggca tggcgatcgc
     1141 agcgctcgcc aatgccggca tggtgttccg caaggcgcag tggacggcgg ccgccatccg
     1201 cgccttcgcc ttcatcgaat ccgcgctcgg cgacggcgac cggctctatc attcgtggcg
     1261 caacggcacg cgccaccaca ccggattctc cgacgactac gcccatatgg cgcgggcggc
     1321 gttcacgctc tgggaggcga ccggcgacaa gcgctatctc gggcgcgcgc aggcctgggt
     1381 gcacgtgctg aacgagcatt tctgggattc gcagaatggc ggctatttct acacgtccga
     1441 cgattccgat ccgctgatcg tcagaacccg catcgtcttc gatcaggccg cgccttgcgc
     1501 caacggattg ctggtcggcc tcctgtcgcg cctgcatctg gcgaccgccg acaatctcta
     1561 ccgcgaccgc gccaacgcgc tcatccaggc ctttgcgagc gaagtgtcgc gcgcctttct
     1621 ttcgatgagc tcctacatca atggcctcga agtcgcgatg actgcgctgc agatcgtcgt
     1681 ggtggggcag atcgacaatt ccaggactca tgagctggtg aacgccgccc tggggcggag
     1741 cctgcccaac cgcaccctga tcgtcgtcga tccgcgcgat acgctgccgg ccggccatcc
     1801 cgcccagggc aagacgatgc agaacggcca gcccaccgcc tatgtctgcc agcgcatgag
     1861 ctgttcggca ccgatcacca atcccgtcac gctgtcccag gttctgctgc tgcctgcgcg
     1921 gccgcagggg caggcatgac gaagggggcg catatcgcaa aggacacgat cccatgaccg
     1981 gcaaggacat caccatccgc acggctgacg gctcgttcgg cggctatctg gcgtctccgt
     2041 ctgcgggcag aggcgccggc atcgtggtca tccaggagat attcggcgtc aacgggttcg
     2101 tgcgcgcggt cgcggacgga tttgccgccc atgggtattt cgcactggcg cccgatctct
     2161 tctggcgcat cgaacccggc gtgcagctga gcgacaagac cgacgccgaa tggtcgcgcg
     2221 cgttcgagct gatgggaaaa ttcaacgcag atctgggcgt gaaggacata caggccgcga
     2281 tcgcggaatt gcgaaatacg ccgggctgca gcggaaaggt gggtgccgtc ggctattgcc
     2341 tgggcgggtt gcttgcctat ctcaccgccg cgcgcaccga cgccgatgcg tccgtcgcct
     2401 attacggcgt gaacatccag aagatgctcg gcgaagccgg ttcgatccgc aaaccgctga
     2461 tgctgcacat cgccggaaaa gacgaatatg tcccgtcggc cgcgcagaaa gcgatcgtcg
     2521 atggtctgaa aacctgtccc catgtcacga ttcatctcta tccggagatg gatcacgcct
     2581 tcgcgcgcac cggcggcgcg cattacgacc gcgccaacgc cgaactcgcc aatggccgca
     2641 catccacctt cttgcgccag catctgagct gaccgtgaag gcgatccgct tcgcccgcac
     2701 cggcggtccg gaggttctcg ccctcgagga catcgacctg ccggcgccgc gcgcgggcga
     2761 ggtgcgcgtg cgccataccg ccatcggcgt caatttcatc gacacctatc accgctgcgg
     2821 cctctatccg gtttcgcttc cgtcgggaat tgggctcgaa ggcgccggtg tggttgaaga
     2881 ggtcggcgac ggcgttacgg ctttccggaa aggagaccat gccgcctatt gcaccggacc
     2941 gcttggcgcc tacgccgaag cgaacaatgt tcctgccgat cggctggtga aaatcccttc
     3001 cggaatcgcc ggcgaaaccg cggccgcgtc cctgctcaag ggcatgaccg cgcaatatct
     3061 cctcaaacgc acctattccg tgcagcacgg ccagacgatt cttttccatg ctgcggcggg
     3121 cggcgtgggg tcgatcgcct gtcaatgggc gaaacatctc ggcgcgaccg tgatcggcac
     3181 ggtgggttcg gacgaaaagg tggcgctcgc caaagccaat ggctgtgcgc atgtgctcaa
     3241 cacgggcagc gacgcctgga gccggcgcgt gcgcgagatc acgggcggcg cgggcgtgcc
     3301 cgtcgtttac gactccgttg gcaaagatac gttcgcgggt tcgctcgact gcctcgccgt
     3361 gcgcgggatg atggtgagct tcggcaattc cagcggcgcc gtgccggcct tcgagcccgg
     3421 aacgttgagc gccaaaggct cgctctatct cacccgcccc atgctcttcc actacacgcg
     3481 cagcgccaaa gaactacagg aaacggccga ggatttgttc gcggtgcttt tgtcgggcgc
     3541 cgtcaagatc gctgttcacc agcgcttccc tctcgccgaa gcgcgccggg cccacgaagc
     3601 attgcactcg cgcgccacga cgggcgcgac cgttctcatc ccgtagccgc ggattgaagc
     3661 tggcgtagct gccaggctac gtattcatgc tttcgaagaa atccgaattc gtcttcgacg
     3721 aacgcatttt gtcgagcagg aattcgatcg catcgattgt gcccatcggc gcgaggatgc
     3781 ggcgcagcac ataggtcttg gcgagtgtgc ccttgtcgat gagcaactcg tccttgcgtg
     3841 tgccagagcg caggatatcg atggcgggga acacgcgctt gtcggcgacc ttgcggtcga
     3901 gaatgatttc cgaattgccg gtgcccttga attcctcgaa aatcacttcg tccatgcggc
     3961 tgcccgtgtc gatcagcgcg gtcgcgatga tggtgagcga accgccttcc tcgatgttgc
     4021 gcgcggcgcc gaagaaccgc tttgggcgct gcagcgcatt ggcatccacg ccgcccgtca
     4081 gcaccttgcc cgatgaaggc acgacggtgt tgtaggcgcg gccgagccgc gtgatcgaat
     4141 cgagcaggat cacgacgtcg cgcttgtgtt cgacgagacg cttcgccttc tcgatcacca
     4201 tttccgccac ctggacatgg cgcgtcgccg gctcatcgaa ggtcgaggac acgacctcgc
     4261 ccttcaccga gcgctgcatg tcggtcactt cttccggccg ctcgtcgatc agcagaacga
     4321 tcaggtagca ttcggggtga ttgacggcga tcgccctggc gatgttctgc agaatcaccg
     4381 tcttgccggt gcgcggctgc gcggtgatga gcgcacgctg gcctttgccc tgcggcgcca
     4441 cgatgtcgat gatccggccc gtcttgtcct tgatggttgg atcctcgatc tccaggttga
     4501 gccacttcgt gggataaagc ggcgttagat tgtcgaagtg gaccttgtgc ttgatctttt
     4561 ccggatcctc gaagttgatc gtcgaaacct tgagcagcgc gaaatagcgt tccccgtctt
     4621 tggggctgcg gatgggacca tcgaccgtgt ccccggtccg caggccaaag cggcggattt
     4681 gcgacggcga aacatagatg tcgtcaggtc cggccaggta gttcgattcg ggcgagcgca
     4741 gaaagccgaa accgtcctgc agggtttcga ggacgccgct gcccgtgatc tcgacatttc
     4801 ggtcggcgag ctccttgagg atcgcaaaca gcatgtcctg cttgcgcatc gcggacgcgt
     4861 tttcgatctc gaggtcttcg gcgaacgcca gcagatcggt gggggatttg gtcttcaaat
     4921 cctgcagctt catcgcctgc aggccggatt gcactgtcat ggggattgca cctggaacgg
     4981 aaaccttcgg ggagctgatc cgccgcccgc ggttccggcg agagtttcgg cggtggtatg
     5041 gttgcgcgcc gcgccggcct gtaccggatt caacttcgcg cttgtcttgc gcgcgctgcc
     5101 caacggcgtg aatggggaac taacgtctta taccgggatt tcgtgccgtt gcaaatgatc
     5161 tttctttgcc gcccgggtgg ctggttcaga aaggcttaac gactgccagg atgacgatga
     5221 acaccaggag cgcggcgggg acctcgttcc atatgcggta gaaacgggcc gaatgcgcat
     5281 tctgatccga cgcgaaggcc cgcacccagc gcgcattgag gccatgaacg ccgctcatca
     5341 ccaccaccag ggcgaatttg aggtgaaacc agttttggcg ccaggcatcg gtcgccggca
     5401 gaaaactcag cgtgagcccc agaatccaca ccgcgatcat cgccgggttg acgatcgcac
     5461 gcagcagccg gcgctccatc cgcttgaagc gttcggaatc gggcgatccg gggagcgttt
     5521 cgcagtgata gacaaagagg cgcggcagat agagcattcc tgccatccag gcgatgacgg
     5581 caatcacgtg aagcgctttg atccagagat agaactccgt tgattcgatc gctgcggtca
     5641 ttgccgtgcc tcgcgcatac cgcaaagtgc aagatccggg gggcaaatcc gcgcggcacc
     5701 gcacgtcacg ggccccgcgg acgcaacggc gtgcagaacg agatcgccca gtccgtgaat
     5761 aaatgcctcg ttcgttccga cggtcgccgc gcggagatag tccggcacgc ccgctttgtg
     5821 cgcaagcgcc gcataatcca tatcaagctc gacgagcgtt tcggagtgct ccgagacgaa
     5881 cgcaaccggc acgacgacca ggcccttgcc ctccccgcct gcccgccgaa tctcctcgtc
     5941 tgtcgcgggc ccgatccatt tgagcggccc aacgcggctc tgatacgaaa tccgccaatc
     6001 cagcgcgtcc tctcccagct catgcacaag agcgccaacc gttcgttcga tctgccagcg
     6061 gtatggatcg ccacgatcga caatccgctg tggcagcccg tgcgcggaaa aaagaaggcg
     6121 gtaggagagt tccggccgcc gatccagaag cgagatccgc acgcgctcgg caagacccct
     6181 gatgaatcct tcgttccacg gataacaaca gacacgtttg tgttcggcgg aaagacccgc
     6241 tttcgcggcc gccgcgcacc aggcagaaag ggaagaggct gtcgtcgtcg tggaaaattg
     6301 cggatagagc gggagaagca cgacacagtc gggcgcgaac cccgcaacag ccgcggctgt
     6361 ttcatcactc attggatccc acgcccgcat ggcgacaaag acgcgtgcct cggcgccgcg
     6421 tcgcacgagc tcctgttcga gcgcgcctgc ctgggcaatc gtttgttcga gaagcggcga
     6481 ttttccgccg atccgctcgt agatcgcacg ggcgactggc gcgcgcc
//
LOCUS       DASZMC010000397         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4840993,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000397 DASZMC010000000
VERSION     DASZMC010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4840993"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /locus_tag="VG274_08165"
     CDS             complement(<1..1910)
                     /locus_tag="VG274_08165"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06667.1"
                     /translation="MRILLRFIFAVCAALISLAPAAQAMVAPSLFQELKWRSIGPFRG
                     GRVLAVEGVPREPLHFYFGAVNGGVWETRDAGRTWSPIFDAAGAGSIGALAVAPSNPA
                     IIYVGTGEADMRSDIAQGIGMFRSDDAGAHWRAIGLTDTQQIGKIVVDPRNPNIVLVA
                     ALGHPYGPNAERGVFRSTDGGKSWTKTLFRNADTGAIDLVFRPRNPDIVYAALWQTRR
                     PPWNVYPPSNGAAGGLYKSTDNGRSWNQIVGHGLPSALGRIGLAVAPTQPDRVFALVD
                     AKEGGLYRSDDAGANWAKIGTDPRIWERGWYFAGITVNPKNPDEIYVCNTILLRSGDG
                     GRHFVPIEGDPTGDDFHTLWIDPANPDRRILGSDQGAQVTLNGGVTWSSWYNQPTGQF
                     YHVVTDNRFPYRVYGAQQDSGAAGVPSRTNGIDGIAMPEFHEIAAGGESDNIAPDPDD
                     PDIIYGGRVDRLDLRSGQLQSVDPTLAFPGNYRGTWTLPLVFGKEDRALYFGNQRIFR
                     TADGGRHWQPISPDLTRPNPGVPANLDAAAAADEEHIGPRRGVVYTIATSPRDARLIW
                     AGTDDGLVWRTVDGGAHWRDVTPKGLAPWSKIGTVEASHFDQDVAYAAVDRHRLDDFA
                     PCIFRTRDGGKS"
     gene            2212..2613
                     /locus_tag="VG274_08170"
     CDS             2212..2613
                     /locus_tag="VG274_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HWE06668.1"
                     /translation="MSIEPRRRSFGKPSRNVFGGTLESGCTNPMTGFFRNGCCDTGAG
                     DGGSHTVCVIVTEAFLKFSASHGNDLSTPLPEYGFPGLKEGDRWCLCAPRWQEALEAG
                     RAPRVVLRATHEDALNYCALADLKRHAIDLA"
BASE COUNT          525 a          922 c          905 g          485 t
ORIGIN      
        1 cagcttttcc cgccgtcgcg cgtgcgaaag atgcaaggtg cgaaatcatc gagccggtgt
       61 cggtctaccg cggcgtaggc aacatcctgg tcgaaatgcg aggcctcgac ggttccgatc
      121 ttcgaccatg gcgcaaggcc cttcggcgtg acgtcccgcc aatgcgcgcc gccatcaacg
      181 gtccgccaga caagcccgtc atcggtgccc gcccagatca ggcgcgcatc gcggggcgat
      241 gtcgcgatcg tgtagacaac gccgcgccgc ggcccgatat gttcttcatc tgccgcggcg
      301 gcggcatcga ggttggccgg cacgccggga ttgggccgcg tgagatcggg gctgatcggc
      361 tgccaatgcc ggccgccgtc cgcggttcga aagatcctct ggttgccaaa atagagcgcg
      421 cggtcctctt tgccgaaaac cagcggaagc gtccaggtgc cgcgatagtt gccgggaaag
      481 gcgagcgtgg gatcgacgct ctgcaattgc cccgaacgca gatcgagcct gtccacgcgg
      541 ccgccataga tgatgtccgg atcgtcggga tcgggtgcga tattgtcgct ttccccacct
      601 gccgcgatct cgtgaaattc gggcatcgcg attccgtcga tcccgttggt gcggctcggc
      661 acgcctgcgg cgcccgaatc ctgctgcgcg ccgtagacgc ggtagggaaa gcggttgtcg
      721 gtcacgacgt gataaaactg gcccgtcggc tgattgtacc atgagctcca tgtaacaccg
      781 ccgttcagcg tgacctgcgc gccctggtcg ctgcccagaa tccggcgatc gggattggcc
      841 ggatcgatcc agagcgtgtg gaaatcgtcg cccgtgggat cgccctcgat cggcacgaaa
      901 tgacggccgc cgtcaccgga acgcagcagg atcgtgttgc agacatagat ctcgtcggga
      961 ttcttcgggt tcaccgtgat gcccgcgaaa taccagccgc gctcccagat gcgcgggtcc
     1021 gtgccgatct tcgcccaatt cgcgcccgca tcatccgaac ggtagagccc gccttctttc
     1081 gcgtcgacga gagcaaagac gcgatcgggc tgcgtcggcg cgacggccag gccgatgcgc
     1141 cccagggcgg acggcaatcc gtgaccgaca atttgattcc agctgcggcc attgtctgtc
     1201 gatttgtaga ggccgcctgc cgcaccgttc gaaggcggat agacattcca gggcggacgc
     1261 cgtgtctgcc acagcgccgc atagacgatg tcgggattgc gtggccggaa gacgagatcg
     1321 atcgcccccg tatccgcatt cctgaacagg gttttcgtcc agctcttgcc gccatcggtg
     1381 gaacggaaca cgccgcgttc tgcattgggg ccatagggat ggcccagggc tgcgaccagc
     1441 acgatgtttg ggttccgcgg atcgacgacg atcttcccga tctgctgcgt gtcggtgagc
     1501 ccgatcgcgc gccagtgcgc gcccgcatcg tccgagcgaa acatcccgat tccctgagca
     1561 atgtcggagc gcatgtcggc ttcgcccgtg ccgacataga tgatcgccgg attcgaaggc
     1621 gcgaccgcca gcgcgccgat cgaacccgcc cccgccgcgt cgaagatcgg cgaccaggtc
     1681 cgccccgcat cgcgcgtttc ccacaccccg ccgttgaccg ctccgaaata gaagtgaagc
     1741 ggctcgcgcg gcacaccttc cacggcgaga acccgcccgc cgcgaaacgg tccgatcgag
     1801 cgccatttga gttcctggaa cagcgacgga gcgaccatcg cctgcgcggc cggcgcgagc
     1861 gatatcaacg cggcgcatac tgcgaagatg aaacggagaa gaattctcaa gccctcagcc
     1921 ctgaaggatc gttgccgccc gcaatgtgac ggcgagtcgg cgcggcttgc aactggagca
     1981 cgatcgaaga aatgaatcac gtgcgatacg ccgtggcccc ctctcgctgc gctcggcacc
     2041 tcccccacgc ttcgcatgag ggaggaaagg aaccgccgag ccctaacgct ctttcctccc
     2101 ccgcgcaaag cgtgggggag gtgcccgcga agcgggcgga gggggaccgc ggcgcaactt
     2161 cgacgccttg cctcggcggt atcggcgtct acttttcgga ggagcaaaat gatgtccatc
     2221 gagccgcgcc gcaggagttt cggaaagccg tcgcggaatg tgttcggcgg cacgctcgag
     2281 agcggttgca ccaatccaat gaccggcttc ttccgcaatg gctgctgcga cacaggcgcc
     2341 ggggacggcg gcagccatac cgtgtgcgtg atcgtgaccg aagcgttcct gaagttctct
     2401 gcctcccacg gcaacgatct ctccacaccg ctgccggaat atggctttcc gggcctgaag
     2461 gagggcgatc gttggtgcct gtgcgcgccg cgctggcagg aagcgctcga agcgggtcgc
     2521 gcgccgcgcg tcgtgctgcg cgccacgcac gaggacgcgc tcaattattg cgcactcgcc
     2581 gatctgaagc ggcacgcgat cgacctggcg taaagtggtt cgaatgaaat aaataaggtg
     2641 tcatggccag cgcaagcggg ccacccagct gagagcgacg agcggcccac gcggcaacaa
     2701 gaactcgcgc gtacaacctc cgcaccttgt aagtttcagc cggttggccc gctctgcggc
     2761 gggccatgac acaatatggt gcgtaaattg aattcgaacc acttccaaac cgtggctcgt
     2821 cgcgagcgag gaaacaa
//
LOCUS       DASZMC010000398         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_740013,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000398 DASZMC010000000
VERSION     DASZMC010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740013"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /locus_tag="VG274_08175"
     CDS             <1..2099
                     /locus_tag="VG274_08175"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HWE06669.1"
                     /translation="RHGQGYSKFEHASRGIALEMTQFVPLDDPIKITRLKITNQSMRE
                     RRLSITAYVEWVLGVDRSACAPFVMTEIDSATGAIFAQNPWNEQFGERVAFADLKGRQ
                     NSWTCDRAEFLGRDGTLDRPLALTPGASLSNRAGAGLDPCGALQTLVRLGAAGKTEVV
                     FFLGQTANRAEAQALIAKYRGADLDAVFDNVTRHWDETLGAVQVKTPDRALDILLNCW
                     LPYQTLACRVWARAGFYQASGAYGFRDQLQDVMALCLSRPEIARAHLLRAAARQFVEG
                     DVQHWWLPESGRGTRTRVSDDCGWLAYVVAHYVQVTGDSAVLDEMVPFLEGPALREGA
                     HDDYFLPKTSEKRASLFDHCALALDRSLRTGAHGLPLMGTGDWNDGMDMVGAGGKGES
                     VWLGWFLHAAITAFSNLAERRGNAEHAQEWRQHAADLREALEREAWDGDWYRRAFFDD
                     GSPLGSVSNNECRIDSIAQSWSVISRAADPLRASRAIAAVDKYLVRRDEKLSLLFTPP
                     FDHPAEDPGYIKGYPPGIRENGGQYTHGATWAVQAFAMQGDGDRAGELLSMLNPIHHA
                     DDAAGVHRYKVEPYVVCADVYSVPPHVGRGGWTWYTGSAGWMYRVALEWLLGLRVQGE
                     NLAIDPCIPRSWPGFEIVYRHRSARYQITVENPLGVCRGILATKLDGEILTGNNRLLM
                     PLLDDGAVHTMQVILG"
     gene            2107..3750
                     /gene="pgm"
                     /locus_tag="VG274_08180"
     CDS             2107..3750
                     /gene="pgm"
                     /locus_tag="VG274_08180"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HWE06670.1"
                     /translation="MAEKTDPLAGKPAPPSLLVDVPQLVTAYFVLTPDPDVPGQRVAF
                     GTSGHRGSAFASAFNEAHILAIAQAVCLYRRHAGIGGPLFLGMDTHALSRPAFASALE
                     VLAANGVETMIDAQGGFTPTPVISHAILTYNRGRGTGLADGIVISPSHNPPDEGGFKY
                     NPANGGPADIDVTGWIERCANDLLAKKLGGVTRIPYERARKSAHIHEYDYVGPYVADL
                     ARVVDMDCIQSAGVRIGIDPLGGAAVHYWQPIIERYGIAATVVNEAVDPTFRFMTVDW
                     DGKIRMDCSSPYAMTRLVAMRDRFDIAFANDTDADRHGIVTPTGGLMNPNCYLAAAIA
                     YLCSNRPGWRGDSAVGKTVVSSGMIDRVAAKLKRRLIEVPVGFKWFCGGLIDGSLAFG
                     GEESAGASFLRRDGSVWTTDKDGLVPGLLAAEITARTGMDPSRLYDSVTADLGKSFYR
                     RVDAPASREQKDKLSKLDAEQFDAKELGGEPIEAKLTRAPGNNQPIGGIKVTARNGWF
                     AARPSGTEDVYKIYAESFRSEQHLRQVQSDAQTAIERLF"
BASE COUNT          719 a         1196 c         1209 g          702 t
ORIGIN      
        1 tgcggcacgg gcaaggttac agcaaattcg agcatgcgtc gcgcggcatt gctctggaaa
       61 tgacgcaatt tgtgccgctc gacgatccga tcaagatcac ccggctgaag atcaccaacc
      121 agtccatgcg cgagcgccgc ctttcaatca cggcctatgt cgaatgggtt ctgggcgtcg
      181 accgcagcgc ctgtgcgccg ttcgtcatga ccgaaatcga ttcggcaact ggcgcaatct
      241 tcgcccagaa tccctggaac gagcagttcg gcgaacgggt ggcgtttgcc gatctcaaag
      301 gacgtcaaaa ctcctggacc tgcgaccgcg cggagtttct cggccgcgac ggcacattgg
      361 accggcctct cgccttgact cccggcgcga gtctttccaa tcgcgccggt gcgggactcg
      421 acccgtgcgg cgcgctgcaa acgcttgtgc gcctcggcgc cgcgggcaaa acggaggtcg
      481 ttttctttct cgggcagacg gcgaacaggg ccgaagcaca ggcgttgatc gcaaaatacc
      541 gcggcgccga tctcgatgcg gtattcgaca acgttacgcg gcattgggac gagacgttgg
      601 gtgcggtgca ggtcaagacg ccggatcgtg cgctcgatat acttctgaac tgctggcttc
      661 cctatcaaac gctcgcatgc cgtgtctggg cgcgcgccgg attctatcag gcaagcggag
      721 cctacggctt ccgggaccag ttgcaggatg tgatggcgct ttgcctgtcc cggccggaaa
      781 tcgcacgcgc ccacctgctg cgcgcagccg ctcgccagtt cgtagaagga gatgtccagc
      841 actggtggct gccggagagc ggacgcggca cccgcacgcg cgtctccgac gattgcggct
      901 ggcttgccta tgtcgtcgcc cactatgtgc aggtgacggg cgacagcgcc gtgctggatg
      961 aaatggtgcc gtttctggag ggcccggcgc tgcgcgaggg cgcgcatgac gactacttcc
     1021 tgccgaagac gtccgaaaag cgcgcgagcc tgttcgacca ttgcgcgctc gcgctcgacc
     1081 ggagtctcag aaccggcgcg catggcttgc cgctgatggg caccggcgac tggaacgacg
     1141 gcatggacat ggtcggcgcg ggcgggaaag gcgaaagtgt ctggctcggc tggtttctcc
     1201 atgccgcgat cacggcattc tcgaaccttg cggaacgtcg tggcaatgca gagcacgcgc
     1261 aggagtggcg ccagcatgcc gcggacctga gagaggcgct cgagcgcgaa gcctgggatg
     1321 gcgactggta tcgccgcgcc tttttcgacg acggttcgcc gcttggttcg gtttccaaca
     1381 acgagtgccg aatcgactcc atcgcgcaat cctggtcggt gatctcgcgc gccgccgatc
     1441 cgttgcgcgc atcccgggcc attgccgccg tcgacaaata tcttgtgcgc cgcgacgaaa
     1501 aattgtcgct gctgttcacc ccgccgttcg atcatcccgc ggaggacccc ggctacatca
     1561 agggctatcc tccgggcatt cgagagaatg gcgggcaata tacccatggc gcgacatggg
     1621 ctgtgcaggc ttttgcgatg cagggcgacg gagaccgggc gggcgaattg ctgtcgatgc
     1681 tcaatcccat tcaccacgcc gacgacgccg ctggcgttca ccgctacaag gttgagccct
     1741 atgtggtctg cgccgatgtc tattccgttc cgcctcatgt cgggcgcggg ggctggacat
     1801 ggtacacggg ctcggcagga tggatgtatc gtgtcgccct ggaatggctc ctgggactgc
     1861 gcgtgcaggg cgagaacctg gcgatcgatc cgtgcatccc gcgctcgtgg cccggcttcg
     1921 agattgtgta tcgtcaccga tccgcgcgct accagattac cgtggagaat ccgctcggcg
     1981 tgtgtcgggg cattctcgcg accaagctgg acggtgaaat cctgaccgga aacaacagac
     2041 tcctcatgcc cctgctggac gacggcgccg tccacacgat gcaggtgatt ctgggataag
     2101 agcggcatgg cggaaaaaac agatcctctt gccgggaagc cggcgccgcc atcgctgctt
     2161 gtcgatgtcc cgcaactcgt gacggcctat ttcgttctca cacccgatcc ggatgttccg
     2221 gggcaacggg tcgccttcgg cacgtcgggg catcgcggct cggctttcgc gtccgctttc
     2281 aacgaagcgc acattctcgc cattgcccag gccgtctgcc tgtaccgccg ccatgcgggc
     2341 atcggcgggc ccctgtttct cggcatggat acgcacgccc tgtcccggcc cgccttcgcc
     2401 agcgccctgg aggtgcttgc agccaacggc gtggagacga tgatcgatgc gcagggcgga
     2461 ttcacgccaa cgccggtgat cagccacgca atcctcacct acaatcgtgg acgcgggacc
     2521 ggcctcgccg atggcatcgt catcagcccg tcccacaatc cgcccgacga gggcggcttc
     2581 aaatacaatc cagcgaatgg cggaccggcc gacatcgatg tgacaggatg gatcgaaaga
     2641 tgcgcaaacg atcttctcgc gaagaagctc ggcggtgtga cgcgcatccc ctatgagcgc
     2701 gcgcggaaat cggcgcatat ccacgaatac gactatgtcg ggccttacgt cgccgacctc
     2761 gccagggtcg tcgatatgga ctgcatccag tccgccggcg tgcgcatcgg catcgatccg
     2821 ctcggcggcg ccgcggtcca ctactggcaa ccgatcatcg agcgctacgg catcgccgcg
     2881 acggtcgtca atgaggccgt cgatccgacg ttccgcttca tgacggtcga ctgggacggc
     2941 aagatcagga tggactgttc atcaccctat gcgatgacac ggctggtcgc aatgcgtgac
     3001 aggttcgaca tcgccttcgc aaacgatacg gatgccgatc gccacggaat tgttaccccc
     3061 accggcggcc tgatgaaccc aaattgttac ctggccgccg caattgcgta tctatgttcc
     3121 aaccgtcccg ggtggcgcgg cgacagcgcg gttggaaaaa ctgttgtttc cagcggcatg
     3181 atcgaccgtg tggcggcaaa actgaaacgc cgacttatcg aagtgccggt cggcttcaag
     3241 tggttttgcg gcggtctgat tgacggatcg ctggcattcg gcggcgagga gagcgccggc
     3301 gcgtcatttc tccgtcgcga cggatcggtg tggacaaccg acaaggacgg actcgttccg
     3361 ggattgctcg cggctgagat caccgcgcgc accggcatgg acccgagcag gctctatgac
     3421 agcgtcaccg ccgatcttgg aaaatccttt tatcgccgcg tcgacgcgcc ggcttccagg
     3481 gaacagaaag acaagctctc caaactcgat gcggagcagt tcgatgcaaa ggaactcgga
     3541 ggcgaaccca ttgaagcaaa gctgaccagg gcaccaggca acaatcagcc gattggcgga
     3601 atcaaggtga ccgccaggaa cggatggttc gccgcgcggc cctccggtac ggaagacgtc
     3661 tacaagatct atgcggagag tttccggagc gaacagcacc tgcggcaagt ccaaagcgac
     3721 gcccagaccg cgattgagcg cctcttctaa ttccgcaagg aggctttctt ccgcagggag
     3781 cgccggttcc cggtaggaag actggagggc gctaccggca aatacg
//
LOCUS       DASZMC010000399         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2376582,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000399 DASZMC010000000
VERSION     DASZMC010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376582"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VG274_08185"
     CDS             <1..356
                     /locus_tag="VG274_08185"
                     /inference="COORDINATES: protein motif:HMM:NF017806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
                     /protein_id="HWE06671.1"
                     /translation="MIVVADSSKLVDRLGRFPLPAEVALFGHSTTARRIAEAAARLGY
                     PGPRPSLRTIGDAPVVTDSGNVIYDCAFVSIADARALAASLSGIPGVVDHGLFIGLCS
                     ALVIAGADGVSVIEA"
     gene            360..1742
                     /gene="gor"
                     /locus_tag="VG274_08190"
     CDS             360..1742
                     /gene="gor"
                     /locus_tag="VG274_08190"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HWE06672.1"
                     /translation="MSRKYDFDLFVIGGGSGGVRASRLAAQKGARVAIAEEYGFGGTC
                     VIRGCIPKKLLVYASHFGEDFEDAAGFGWTVPERQFDWNVLIANKNKEIARIEGVYRE
                     AVEKAGAEVFMERAEFEDAHTMHLKRSGRRITAANFLIATGGHPSRNMGSAPGAEHCI
                     TSNEAFYLEDFPRRIVIAGGGYIAIEFAHIFHGLGAEVTLVYRGPKLLRGFDEDMRDA
                     VTKSSEARGIRVVLDRMFTKVEKSGNEVFAFTDKNEMLEADQVMLAIGRAPNTDGMGL
                     ARIGVELGKRGQIKVDEFSRSSQSHIYAVGDVTDRLALTPVAIHEAVAFVRTVFDNDP
                     TPVDHVNVPTAVFSQPEIGTVGLSEAAALARGFAIDVYKANFRPLKNTLSGRAERALF
                     KLVVDARTDRVLGCHLLGPEAAEIIQFVAVAMKMGATKSNFDATVALHPSLAEELVTM
                     HAKAYSKGPE"
     gene            complement(1746..>2411)
                     /locus_tag="VG274_08195"
     CDS             complement(1746..>2411)
                     /locus_tag="VG274_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06673.1"
                     /translation="WTRLSGTTGVSAVKGGPRLFFISGHMKRSIGVAMAVAVAGCGTV
                     SNFRYDINSGSFNDRTKAVIQSCETRMRAPALDPIRAKVELFKSPADGPAPFAILTNS
                     GLPDASDRRAIDLWAQAVEQCQAQARLLIAAIPVPPDATQLEVDKLAAYITDAWIESS
                     KLRVALYNGQLTYADYASQRVKIVEDALRTASRYAQDMDEENGTHALEDSETALERFA
                     SMM"
BASE COUNT          423 a          752 c          798 g          438 t
ORIGIN      
        1 gcatgatcgt ggtcgcggat tcgtccaagc tggttgaccg gctcggccgc tttcccctcc
       61 ccgcggaagt cgcgctgttt gggcactcga cgaccgcgcg gcgcatcgcc gaagcggcgg
      121 cgcgactcgg ctatccggga ccgcggccgt cgcttcgcac catcggcgat gcgcctgttg
      181 ttaccgacag cggcaatgtg atctacgact gcgccttcgt ctccatcgcg gatgcgcgcg
      241 cgctcgcggc ttcgttgagt ggaatccccg gcgtggtaga ccatggcctc ttcatcggcc
      301 tttgttccgc gctggtgatc gcgggggcgg acggcgtaag cgtgattgag gcttgagtga
      361 tgtcgcggaa gtacgatttc gatctgttcg tcatcggcgg aggttcgggc ggggtgcgcg
      421 cgagccggct tgcggcgcag aaaggcgcgc gcgtcgcgat cgccgaggaa tacggcttcg
      481 gcggcacctg cgtcatccgc ggctgcattc ccaagaagct gctcgtctat gcgagccatt
      541 tcggcgagga tttcgaggat gccgccggat tcggctggac cgtgcccgaa aggcagttcg
      601 actggaatgt gctgatcgcc aacaagaaca aggagatcgc gcgcatcgag ggcgtctacc
      661 gcgaagcggt cgagaaggcg ggcgccgagg tgttcatgga gcgcgcggaa ttcgaggacg
      721 cgcatacgat gcacctcaag aggagcggac ggcgcatcac ggccgcaaat tttctgatcg
      781 ccactggcgg gcatccttcg cgcaacatgg gcagcgcgcc gggcgcggag cattgcatca
      841 cctccaacga ggccttctat ctcgaagatt tcccgcggcg catcgtcatc gccggcggcg
      901 gctatatcgc gatcgaattc gcccacatct tccacggact gggcgccgag gtgacgctcg
      961 tctaccgggg ccccaaactg ctgcgcggct tcgacgagga tatgcgcgac gcagtaacga
     1021 aatcgagcga ggcgcgcggc atccgcgtgg tgctcgaccg gatgttcacc aaggtcgaaa
     1081 aaagcggcaa cgaagtcttt gccttcaccg acaagaacga aatgctcgaa gccgaccagg
     1141 tcatgctcgc cattggccgc gcgcccaaca cggacggcat gggcctcgcg agaatcggcg
     1201 tcgagcttgg caagcgcggg cagatcaagg tcgacgaatt ttcacgctcg agccagtccc
     1261 atatttacgc cgtcggcgac gtgaccgaca ggctggcgct cacgccggtg gcgatccacg
     1321 aagcggtggc gttcgtgcgg acggtgttcg acaacgatcc gacgcccgtc gatcacgtga
     1381 acgtgccgac ggcggtgttc agccagcccg agatcggaac cgtcggattg agcgaggctg
     1441 ccgcgctcgc gcgaggcttc gcgatcgacg tctacaaggc gaatttccgc ccgctgaaaa
     1501 acacgctgtc gggccgggcg gaacgcgcgc tgttcaagct cgtggttgac gccaggaccg
     1561 accgggttct gggctgtcac ctgctcgggc cggaagccgc cgagatcatc cagtttgtcg
     1621 cggtggcaat gaagatgggc gccacgaaat cgaatttcga tgcaaccgtc gcgctgcacc
     1681 cgagcctcgc cgaagaactc gtgaccatgc acgcgaaggc gtattcgaaa ggccccgagt
     1741 agccctcaca tcatcgacgc aaagcgctcg agcgccgttt cggaatcttc gagagcgtgg
     1801 gtgccgttct cttcgtccat gtcctgggcg tagcgcgagg cggtccgcaa cgcgtcctcc
     1861 acgatcttca cccgctggct cgcataatcc gcataggtca actgtccgtt gtagagcgcc
     1921 acgcgcagct tgctgctctc gatccaggcg tcggtgatgt aggcggcgag cttgtcgact
     1981 tccaattgcg tggcgtccgg cggcaccgga atcgcggcga tgagcaggcg cgcctgcgcc
     2041 tgacattgct cgacggcctg cgcccacaga tcgatcgccc ggcggtcgct cgcatcgggc
     2101 aggcctgaat tggtcaggat cgcgaagggc gccggtccgt ccgccggcga tttgaagagt
     2161 tcgaccttcg cgcggatggg gtccagggcc ggggcgcgca tccgggtttc gcaggattgg
     2221 atgacggcct tcgtgcggtc gttgaagctg ccggaattga tatcatatcg gaagttgctg
     2281 acggtgccgc atcccgccac ggcaaccgcc atcgccaccc ctatcgatct tttcatgtgt
     2341 ccgctgatga agaagagccg cggcccgcct tttacggcag agaccccggt tgtgccagat
     2401 aggcgcgtcc a
//
LOCUS       DASZMC010000400         5107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5393844,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000400 DASZMC010000000
VERSION     DASZMC010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5393844"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..860)
                     /locus_tag="VG274_08200"
     CDS             complement(54..860)
                     /locus_tag="VG274_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06674.1"
                     /translation="MTARIHSGEISSMSLQFQMHVPRVCLTLALLVAASAASAGPPYL
                     TDDPETPPLHRWEAVLFTMGTLADGTASGVLPAIEFNYGGFEATQLHIMVPIGFRAGG
                     HDGTEFGGADIELGVKYRFVEEDEQGWRPQVAVYPQFEIPTQSSRNGLSTAGVDVFLP
                     AWVQKNLDENWTADAGGGYWINPGSGNRNYWFSGFLLQRQLGDALVAGVEAFHQTSST
                     LGGRASSGFNLGAVYDLDEHNHVLLSAGRGFENAAVTGQFTWYVGYKITN"
     gene            927..1556
                     /locus_tag="VG274_08205"
     CDS             927..1556
                     /locus_tag="VG274_08205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWE06675.1"
                     /translation="MRKGFVAFIAAILVLGATGVGHAAPADQIVIDTSMGDITIALDR
                     AHAPMTVDNFLRYVNEGHFDGTLVYRVVPGFVIQAGSYDSATHQRPVYGPIPLEANNG
                     LSNVRGTIAMARENDPNTATAEFFINLADNLRLDHHADDPGNATGYTVFGRVVSGMDV
                     VDKIASVPLGDGGPMAGAWPLEPATIRKVRLLATPAALPPAAASPAPSL"
     gene            1797..2891
                     /gene="recA"
                     /locus_tag="VG274_08210"
     CDS             1797..2891
                     /gene="recA"
                     /locus_tag="VG274_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWE06676.1"
                     /translation="MMKEAVLRVVGREEKSDRKRALEAALSQIDRAFGKGSVMKLGQR
                     EVVETDTVSTGSLGLDIALGIGGLPRGRVIEIYGPESSGKTTLALHAVAEAQKKGGIA
                     AYIDAEHALDPIYARKLGVDIDEMLISQPDTGEQALEIADTLVRSGGVDIVVIDSVAA
                     LTPKAELEGEMGDQLPGSQARLMSQALRKLTGSISKSNSLVVFINQIRMKIGVMFGNP
                     ETTSGGNALKFYASVRLDIRRIGAIKDRDEVIGNQTRVKVVKNKVAPPFKEVEFDIVY
                     GSGISKVGELIDLGVRAGIVEKSGAWFSYDGIRIGQGRENAKQYLIQNGDAAARIEAQ
                     IRQNAGLIGKSLMEAPVAKELESAAIAAED"
     gene            3061..>5107
                     /gene="alaS"
                     /locus_tag="VG274_08215"
     CDS             3061..>5107
                     /gene="alaS"
                     /locus_tag="VG274_08215"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWE06677.1"
                     /translation="MNSLSEIRSTFVDFFRDRGHEPVPSSPLVPRNDPTLLFTNAGMV
                     QFKSVFTGQDRRSYARAVTAQKCVRAGGKHNDLDNVGYTARHLTFFEMLGNFSFGDYF
                     KERAIAYAWELVHKVFALPAERLIVTVYSEDDEAFGLWKKISGLAESKIIRIPTSDNF
                     WSMGDTGPCGPCSEIFFDHGSQFAGGPPGCADQDGDRFVEIWNLVFMQYEQVAGGRRE
                     PLPRPSIDTGMGLERFATVMQGVHDIFDVDLFRHLIAASVELTDRPVSASHRVIADHL
                     RSAAFLIADGVLPSNEGRGYVLRRIMRRAMRHAHLLGARDPLLYRLLPVLVAEMGGAY
                     PELKRAEALTSETLRIEELRFRDTLARGLKLLDEASEGLSRGDRLAGETAFRLYDTYG
                     FPLDLTEDALRQRGISVDTAGFAQAMEQQRAEARKAWSGSGEAGTDAQWFALRDEIGS
                     TEFLGYDTEEAEGTIVAILKDGARVERARAGEIVRILTNQTPFYAEAGGQIGDRGAMF
                     SAKGGEGRVTDTEKKLGALHVHVVEVDRGTLGVGDVVELRVDGERRRATRANHSATHL
                     LHAALKRELGPHISQKGSLVAPDRLRFDFSHPKPLTPDELERVETDVNAVIRQNSDVA
                     TRLMPTRDAIAAGAEALFGEKYGDEVRVLAMGEAGTKAYSVELCGGTHVRRLGDIGLF
                     "
BASE COUNT          945 a         1602 c         1629 g          931 t
ORIGIN      
        1 tccccctccg gcccttcggg ccacctcccc cacgttgggg gaggaaagct ctttcaattg
       61 gtgatcttgt agccgacgta ccatgtgaat tgaccggtga cggctgcgtt ctcaaatccg
      121 cgtcccgccg agaggagtac atgattgtgc tcgtcgagat cgtagacggc gcccagattg
      181 aatccggagc tcgcgcgtcc gccgagcgtg cttgacgttt gatgaaatgc ctcgactcct
      241 gccacgagcg catcgccgag ctggcgctgc agaagaaagc ccgagaacca gtagttgcga
      301 ttgccggatc ccggattgat ccaatagccg ccaccggcat cggcggtcca gttttcatcc
      361 aggttcttct gtacccaggc gggcaggaac acgtccacgc cggcagtgga aagtccgttc
      421 cggctcgatt gcgtcggaat ctcgaattgc ggatacaccg ccacctgcgg gcgccagccc
      481 tgctcatcct cctcgacgaa tcggtatttg acgcccagct cgatatcggc tccgccgaac
      541 tccgttccgt cgtggccacc cgcccgaaac ccgatcggca ccatgatgtg cagctgcgtg
      601 gcctcgaacc cgccataatt gaattcgatc gccggtagaa ctccgctcgc cgtgccgtcg
      661 gcgagcgttc ccatcgtgaa cagcacggct tcccagcgat gcagcggcgg cgtctcggga
      721 tcgtcggtga ggtagggagg acccgcgctc gcggccgatg ccgcgacaag aagcgcgagg
      781 gtcaggcaga cgcgcggaac atgcatctgg aactgaaggc tcatggagga gatttcgccc
      841 gaatgaatgc gtgctgtcat ctctgccgag gcgccacgag gcgaaaaatc gcgctatgat
      901 ggagcggcaa tcgggagagc attctcatgc ggaaaggttt cgtcgccttc attgccgcca
      961 ttcttgtgct tggcgccacg ggcgtcggcc atgccgcgcc cgcggatcag atcgtgatcg
     1021 acacttcgat gggcgacatc accattgcac tcgaccgggc gcacgcgccc atgaccgtcg
     1081 acaattttct gcgctatgtg aacgaagggc atttcgacgg cacgctcgtc taccgcgtgg
     1141 tgcccgggtt cgtgatccag gcgggcagtt acgattcggc cacgcaccag cggcctgtct
     1201 acggtcccat tcccctcgaa gcgaacaatg ggctcagcaa tgtgcgcggg acgatcgcca
     1261 tggcgcgcga gaatgatccc aataccgcga cggcggagtt cttcatcaat ctcgccgaca
     1321 atctgcggct cgatcatcat gccgacgatc cggggaacgc caccggctac accgtgttcg
     1381 gccgcgtggt ctcgggcatg gacgtcgtcg acaaaatcgc atccgtcccg ctcggcgacg
     1441 gcggtccgat ggccggcgcg tggcccctcg agccggcgac aatccgcaag gtgaggctcc
     1501 tggcgacgcc ggcggccctt ccgcctgccg ccgcgtcccc ggccccgtct ctctgatcgc
     1561 cgggccggcg attcaaaggc ccgacaaacc cctccccaac gctgggccaa agcgtgaacc
     1621 tttgcagcgc ccgatccgcc cccatggaaa cgtgaactct tcccgaacgt agatagaatt
     1681 atactgcaac ttcaatgtat tatcctgtga cttgcgaaca ggaacaaacc atgtacagta
     1741 cacgtcctgc gacccatttg ggatttgggg agcgctgtga aataaggagg cgagagatga
     1801 tgaaagaagc ggttttgcgg gtggtgggac gcgaggagaa aagcgatcgg aagcgcgcgc
     1861 tcgaagcggc gctgtcgcag atcgaccgtg ccttcggcaa gggctcggtg atgaagctgg
     1921 gccagcgcga ggtggtcgaa accgacacgg tgtcgaccgg gtcgctcggt ctcgatattg
     1981 cgctcggcat cggcggtttg ccgcgcggcc gggtgatcga gatctacggg ccggaatcat
     2041 ccggcaagac gacactcgcg ctgcacgccg tggcggaggc gcagaagaag gggggcatcg
     2101 cggcctatat cgatgcggag catgcgctcg accccatcta tgcgcgcaaa ctcggcgtcg
     2161 atatcgacga gatgctgatc tcgcagcccg acacgggcga gcaggcgctc gaaatcgccg
     2221 atacgctcgt gcggtcgggc ggcgtcgata tcgtcgtcat cgattcggtg gcggcgctga
     2281 cgcccaaggc cgaactcgaa ggcgagatgg gcgatcagct tcccggttcg caggcgcggc
     2341 tgatgagcca ggcgctgcgc aagctgacgg gatcgatctc caaatccaac agcctcgtcg
     2401 tcttcatcaa tcagattcgc atgaagatcg gcgtgatgtt tggcaacccg gagaccacga
     2461 gcggcggcaa cgcgctcaaa ttctacgcct ccgtgaggct cgatatccgc cgcatcggcg
     2521 ccatcaagga tcgcgatgag gtgatcggca accagacgcg cgtcaaggtg gtgaagaaca
     2581 aggtggcccc gccgttcaag gaggtcgagt tcgacatcgt ctatggttcg ggcatttcga
     2641 aagtgggcga attgatcgat ctgggcgtgc gggccggcat cgtcgagaag tcgggcgcct
     2701 ggttttccta tgacggaatc cgcatcggcc agggccgcga gaacgcgaag caatacctca
     2761 tccagaatgg cgacgcggcc gcgcgcatcg aagcccagat tcgccagaac gccggcctga
     2821 tcggcaagag cctgatggaa gcgccggttg cgaaggagtt ggaatcggcc gcgattgcgg
     2881 ccgaagacta gtgtcgttcc tttggcatgg gaacggctcc agaaggggcg ccgcttgcgg
     2941 cgccccttcg cttatctggc ctttgcttat ctggccgacg gcgcctttcg ggccggcgcc
     3001 gctcgcgcga tgtactcatt cccgattcca ctttgctgaa aagcgcttta caagccagtc
     3061 atgaacagcc tgtccgaaat ccgctcgaca ttcgtcgatt ttttccgtga ccgcggccac
     3121 gagccggtgc cgtcgtcgcc gctggtgccg cgcaacgatc cgacactgct cttcaccaat
     3181 gcgggcatgg tgcagttcaa gagcgtgttc accggccagg acaggcgttc ctatgcccgt
     3241 gcggtcaccg cgcagaaatg cgtgcgcgcc ggcggcaagc acaatgacct cgacaatgtc
     3301 ggctacaccg cgcgccatct cacctttttc gagatgctgg gcaatttctc gttcggcgac
     3361 tatttcaagg agcgggcgat cgcctatgcc tgggaactcg tccacaaggt cttcgcgctc
     3421 ccggcggagc gattgatcgt caccgtctat tcggaggacg acgaggcctt cgggctctgg
     3481 aagaagatct ccggcctggc ggaatcgaaa atcatccgca ttcccacttc ggacaatttc
     3541 tggtccatgg gcgataccgg cccctgcggc ccctgctccg agatattctt cgatcacgga
     3601 tcgcaattcg ccggcggccc gccgggctgc gccgaccagg acggagaccg cttcgtcgaa
     3661 atctggaatc tcgtattcat gcagtacgaa caggttgccg ggggacggcg tgaaccgttg
     3721 ccgcgcccgt cgatcgacac cggcatgggg ctcgagcgct tcgccaccgt catgcagggc
     3781 gtgcatgaca ttttcgacgt cgatctgttc cgccatctga tcgcggcttc ggtcgaactg
     3841 acggaccggc ccgtatcggc aagccatcgc gtcattgccg atcatctgcg ctcggccgct
     3901 ttcctgatcg cggacggcgt tctgccttcg aacgaggggc ggggctatgt gctccgccgc
     3961 atcatgcgcc gcgcgatgcg gcacgcgcat cttctgggcg cgcgcgatcc gctgctctac
     4021 cggctcctgc cggtgctggt cgcggaaatg gggggcgcct atcccgagct gaaacgcgcc
     4081 gaggcgctga cctccgaaac gttgcggatt gaggagttgc gcttccgcga tacgctggcg
     4141 cggggattga agcttctcga cgaggcgagc gaagggctct cccgcggcga ccggctggcg
     4201 ggcgaaaccg ccttcaggct ctacgacacc tatggctttc cgctcgatct gacggaggac
     4261 gccctccggc agcgcgggat ttccgttgac accgcgggat tcgctcaggc gatggagcag
     4321 cagcgggcgg aggcgcgcaa agcctggtcg ggttccggcg aagccggcac ggatgcgcaa
     4381 tggttcgcgc ttcgcgacga aatcggttcg accgaattcc tcggttacga caccgaagag
     4441 gccgaaggca caatcgtggc aattctgaag gacggcgcgc gcgtcgagcg cgcgcgagcc
     4501 ggagagatcg tccgtatcct gaccaatcag acgccgttct acgcggaggc gggcgggcag
     4561 atcggcgacc gcggtgcgat gttctccgcc aaaggcggtg agggccgtgt gaccgatacc
     4621 gaaaagaagc tcggcgcgtt gcacgtgcat gtggtggaag tcgatcgcgg gaccctcggc
     4681 gtgggcgacg tggtcgaact ccgggtcgat ggcgagcgcc ggcgtgccac gcgcgccaat
     4741 cattccgcca cccatctcct tcatgccgct ctgaagcgcg agctcgggcc tcatatttcc
     4801 caaaaaggct cgctcgtcgc gcccgaccgg ctgcgtttcg atttcagcca cccgaagccc
     4861 ctcacgcccg acgagctcga acgcgttgaa accgacgtca atgcagtgat tcgccagaat
     4921 tccgacgtgg cgacgcggct gatgccgacg cgcgatgcga tcgcagcagg cgccgaagcc
     4981 ctgttcggcg agaaatatgg cgacgaagtg cgcgtactcg ccatgggcga ggcgggcacg
     5041 aaagcctatt cggtcgaatt gtgcggcggc acgcatgtgc gccggctggg cgatatcggc
     5101 ctgttca
//
LOCUS       DASZMC010000401         4917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2921778,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000401 DASZMC010000000
VERSION     DASZMC010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2921778"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VG274_08220"
     CDS             <1..220
                     /locus_tag="VG274_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HWE06678.1"
                     /translation="GSCTLQPAYGCGAVFKIAPDGTETILYSFTKNNGANGAQPVASL
                     TIDGAGNLYGTASGGGQYGYGTVFEITP"
     gene            237..527
                     /locus_tag="VG274_08225"
     CDS             237..527
                     /locus_tag="VG274_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06679.1"
                     /translation="MGYLDSSPRYLVQAHLVVDECYDAQVRDCGMWHASTAITRSSVG
                     GNVQGFSVIDTHIEGVKTGIFHQHGTTTAGEDPDGRSRRADARRHRHAPSTA"
     gene            850..3354
                     /locus_tag="VG274_08230"
     CDS             850..3354
                     /locus_tag="VG274_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWE06680.1"
                     /translation="MPGDHLSERLEASRNWAARHRLAVMRALAIVAAIFVVPVVALEL
                     APGYLVWTAPSIDPNKDLYAANRPIAFTFLDADGNVAGRRGAIVGQRLSLGDMPAYLP
                     AAFIAMEDRSFYENDGIDIRGLVRAAWMNLRAGHVVAGGSTITQQTAKIVFLSPQRTF
                     ARKYEELIDAAALEKSLSKKQILELYLNRIYLGSGAYGVDGAAHVYFGKSARDLSLSE
                     AAMLATLTRAPSAFSPRRDLAAAQARAGLVLRAMVRTGAITRDQADEAGLHPAVISDL
                     RLADARNFFLDTAADEALKLVGADGEGRDTDLIVHTTLEPRLQEAARHALAHTLNARG
                     RRAHVSEGAIVLMKPDGAVSALIGGRDYDSSVFNRATQAKRQPGSAFKPFVYLAALEN
                     GLTPWDVREDGPVDINGWTPTNYGGRSYGTVTLADALAHSINTVTAGLAQEIGITSVV
                     DAAHRCGISSPLAPNASLALGTSDVTPLELTTAYAVFASGGERVSPYLVTTVEDSAHR
                     TLFQRKPAAPDRVIASHVNRDLTGMLYGVVVEGTGRAAALPGRQAAGKTGTTQDYRDA
                     WFVGFTPDYVAGVWVGNDDNSPMRNVTGGTLPAAIWKDVMTVAERGLPARTLDKSTEP
                     AVEDTSDTESASTSDDETSGESAAASNDSSMREPGANGEAAQGQQQSPAAPGSSSSFW
                     DWLTGNSHDKSGEQPHASAEPANNSPDTTAEAPPAQSVPERRVESRPPAGPDMMDTPP
                     VGEPDADAGPPPPRDLPDEPPAPVVRMHRDERPPPPRDYPDEPPPAIVMMHRDERPPP
                     PPDDIQAEPPPAIVVHHRERLPPPPPPPPDDDGDGN"
     gene            3772..4791
                     /locus_tag="VG274_08235"
     CDS             3772..4791
                     /locus_tag="VG274_08235"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWE06681.1"
                     /translation="MAGWAIRVRAILAAIASAVVISCGAARASTHITFVTDWKAQAEH
                     GGFYEAQALGLYAREGLDVRILEGGPNVNVPQLLAAGAADFGIGSNGFIPLNLLREGA
                     PIKAVMAVFQKDPQVLISHVRADVKSLADMRGKPIMISDASISAFWPWLKARYRFDDT
                     QIRKYTFNLAPFLVDRKAIQEGYLTSEPYTIASQGHVAARVFLLADYGYPGYADMVLV
                     PQKWIVANPRAVQGFVNATREGWRRYLFADPAPANALIKRDNPEMTDGVIAQAIAKMK
                     SYGLVTSGDAASPGIGAMTEARWKIFFETMSSEGLYDRKLPWRNAFDLRFIARGADGP
                     PAAHR"
BASE COUNT          887 a         1637 c         1572 g          821 t
ORIGIN      
        1 cggaagctgt acgctccagc cggcctatgg ttgcggagca gttttcaaga tcgcgccgga
       61 cgggaccgaa acgattctct attccttcac gaaaaacaac ggcgcgaacg gtgcccagcc
      121 tgttgcgagt ctcactatcg atggcgcggg caatctctac ggcaccgcaa gcgggggcgg
      181 ccaatacggc tacggcaccg tcttcgagat cacaccgtga ggccgcgcca gcctcgatgg
      241 gttatctcga ctcttccccg cgctacctcg tccaggcgca tctggttgtc gacgagtgct
      301 acgatgcgca ggtcagggac tgcgggatgt ggcacgcctc gaccgcgatc acccgttcgt
      361 ccgtcggcgg caacgtgcag ggcttttcgg tgatcgacac ccatatcgag ggcgtgaaga
      421 cgggcatctt ccatcagcat ggaacgacca ccgcgggcga ggatccggac ggccgctcgc
      481 gtcgcgccga cgcccggcgt caccgccatg ccccctccac cgcttagttt atcctcgttt
      541 atcctcgggc cggccttcgg ccggacccgg gggcggtccc ccgcgcattc gcgcggggga
      601 ggaaagaata ggcgtacacg atgtgtagat gccctgacgc gcggaccggg gcggcgggcc
      661 atgacacgtt tgggtgcgaa gggtcgggaa tcgaaccacg gacactccga aaaacaaggc
      721 tcaaattgtt cggctttgcg ttatctgcaa ttgaacggcg ggcgcgacaa acgccgccgc
      781 gcgcgatacg cgctgctcgc ctgcgcgtct tgaaactgcc gcattggtcg gcgaggaact
      841 gggaccatca tgccgggcga tcatctgtcg gagaggctgg aggcgtcgcg caactgggcc
      901 gcgcgtcaca ggctcgccgt catgcgcgcg cttgccatcg tcgccgcgat cttcgtcgtt
      961 cccgtggtcg cgctcgagct ggcgccgggc tacctggtgt ggacggctcc cagcatcgat
     1021 ccgaacaagg atctctacgc cgcgaaccgg ccgatcgcct ttaccttcct cgacgcggac
     1081 ggaaacgtcg ccggacggcg cggcgcgatc gtcggccagc gtctctcgct cggcgacatg
     1141 ccggcctacc ttcccgcggc cttcatcgcg atggaggatc gcagcttcta tgaaaacgac
     1201 ggcatcgaca tccgcggact ggtgcgcgcg gcatggatga acctgcgcgc cggtcacgtc
     1261 gtcgccggcg gttcgacgat cacccagcag accgcgaaga tcgtgttcct gtctccccaa
     1321 cgcacttttg cgcgcaaata cgaagagctg atcgatgccg ccgcgctgga gaaatccctc
     1381 tccaagaagc agattctgga actctatctg aaccgcatct atctgggctc cggcgcctac
     1441 ggagtcgacg gcgcggccca tgtgtatttc ggcaagtcgg cgcgcgacct ctcgctttcc
     1501 gaagcggcca tgctggcgac gctgacgcgc gcgccttccg ccttctcgcc ccggcgcgat
     1561 cttgccgccg cgcaggcgcg cgccgggctc gtgctgcgcg cgatggtgcg gacgggcgcg
     1621 atcacccgcg accaggctga cgaagcgggt ttgcaccctg cggtgatcag cgatttgagg
     1681 ctcgccgatg cgcgcaattt ctttctcgat acggcggccg acgaagcctt gaagcttgtg
     1741 ggcgcggacg gcgagggacg cgacacggat ctgatcgtgc acaccacgct cgagccgcgg
     1801 ctgcaggaag cggcgcggca cgccctggcg cacacgctga acgcccgcgg caggcgcgcg
     1861 catgtgagcg aaggcgcaat cgtgctgatg aagccggacg gcgcggtctc cgcgctgatc
     1921 ggcggacgcg attacgattc gagcgtgttc aatcgcgcca cccaggccaa gcgccagccc
     1981 ggctccgcct tcaagccgtt cgtctatctc gcggcgctcg aaaacggcct cacgccatgg
     2041 gatgtgcgcg aggacggccc cgtcgacatc aacgggtgga cgcccaccaa ctatggcggc
     2101 cgcagctacg gcacggtgac gctggccgac gccctcgccc attccatcaa cacggtgacc
     2161 gcaggtctcg cccaggagat cggcatcacc agcgtcgtcg acgccgcgca tcgctgcggc
     2221 atctcttcgc ctctggcgcc gaacgcgtcg ctggcgctcg gcacctccga cgtgacgccg
     2281 ctcgagctga ccactgccta tgcggtgttc gcatcgggcg gcgagcgcgt ctctccctat
     2341 ctcgtgacca cagtcgagga cagcgcgcat cgtacgctgt tccagcgcaa gcccgcggcg
     2401 ccggatcgcg tcatcgcctc gcatgtcaac cgcgacctga cgggaatgct ttacggcgtg
     2461 gtcgtcgaag gaaccgggcg ggcggccgcg cttcccggac gccaggccgc gggcaagacc
     2521 ggcaccacac aggactatcg cgacgcctgg tttgtcggct tcacgccgga ctacgtggcc
     2581 ggcgtgtggg tcggcaatga cgacaattct ccgatgcgca acgtgaccgg cggcaccctg
     2641 ccggcggcga tctggaaaga cgtcatgacc gtggccgagc gcggactgcc ggcaaggaca
     2701 ctggacaaat ccacggagcc tgcggtcgag gacacgagcg acacggaaag cgccagcacg
     2761 tccgacgacg agacgagcgg cgagtcggcc gccgcgtcaa acgattccag catgcgcgag
     2821 cccggcgcga acggcgaggc cgcgcagggt cagcaacaat ctcctgctgc gccgggcagc
     2881 tcgagttcct tctgggactg gctgacggga aacagccacg acaaatcggg cgagcaaccg
     2941 cacgcctccg cggaaccagc aaataattca cccgacacca cagccgaagc gccgccggcc
     3001 caaagcgtgc cggaacgccg cgtggaatcg aggccgcccg cgggcccgga catgatggac
     3061 acgcctccgg tcggcgagcc ggatgccgac gcgggtccgc cgccgccacg cgaccttccc
     3121 gacgagccgc ccgccccagt cgtgaggatg caccgggatg agcgtccgcc gccgccacgc
     3181 gactacccgg acgaaccgcc gcccgcaatc gtgatgatgc atcgcgacga gcgtccaccg
     3241 ccgccgcccg acgacatcca ggccgaaccg ccgccggcga tcgtggtgca tcaccgcgaa
     3301 cgattgccgc ccccgccgcc gcccccgcct gacgatgacg gcgacgggaa ttgatctcgg
     3361 caaattcaaa aaaataagat tcaacgcaga ggccgcagag gatgcgcacg gtgcaggtta
     3421 tgactgactt tatggtcata tcgaggatgc catcgtggtc acatgggcca acgcgcagcg
     3481 cgtgatttat ttcgatacga gcttcctcgc gccgctcgtt cttcaggaag ccacgagcga
     3541 caggattgcc gcctttgtcc gcaagttgct tttggtcgaa ttgtcaaaca aaccaatgtc
     3601 attcccgccg aagcgggaat ccagggcaag cgacgtgcgt tggcccctgg atgcccgctt
     3661 cggcgggcat gacatccctt ttttttcgtg aatgaattgg ttcgccaacg actttcccgg
     3721 aaaccccgaa atgattgacc gggcgcggag ttgctctatc aatctgcctt catggctgga
     3781 tgggcgattc gggttagagc aattcttgcg gcaatagcgt ccgctgtcgt catttcctgc
     3841 ggcgccgcgc gtgcatcaac gcacatcacc ttcgtcaccg actggaaagc gcaggccgag
     3901 catggcggct tctacgaagc gcaggcgttg ggcctctacg ccagagaagg cctcgacgtg
     3961 cgcattctcg agggtgggcc gaacgtgaac gtgccccaat tgctggcggc gggcgcggcg
     4021 gatttcggca tcggctcgaa cggattcatc ccgctcaacc tgttgcgcga aggagcgccg
     4081 atcaaggcgg tgatggcggt gttccagaag gatccgcagg tgctgatcag ccatgtgcgc
     4141 gccgatgtga aatcgcttgc cgacatgaga ggcaaaccga tcatgatctc cgacgcgtcc
     4201 atttccgcgt tctggccgtg gctcaaggcg cgatatcgct tcgacgacac gcagatccgc
     4261 aaatatacct tcaacctcgc gccgttcctc gtcgatcgca aggcgatcca ggaaggctac
     4321 ctgacgagcg aaccctatac gatcgcgagc caggggcatg ttgccgcgcg cgtgttcctg
     4381 ctcgccgact atggctatcc cggctatgcc gacatggtcc tggtgccgca gaaatggatc
     4441 gtggccaatc cgcgcgcggt gcagggtttc gtgaacgcaa cgcgggaagg ctggcgccgc
     4501 tacctgttcg cggatccggc gccggcgaat gcgctcatca agcgggacaa tccggagatg
     4561 acggacggcg tcatcgccca ggcgatcgcg aagatgaaat cctacgggct ggtgacgtcc
     4621 ggcgatgcag cgtcgcctgg catcggggcg atgaccgaag cccgctggaa gattttcttc
     4681 gaaaccatgt cttcggaagg actctacgac cgcaagcttc cgtggcggaa cgctttcgat
     4741 ctgcgcttca tcgcgcgcgg cgcggacggg ccgcctgctg cgcatcgatg acgccgctcg
     4801 tccgcctcga aggcgccggc aagacattcg acaacggcac aaaagctttc gggccgctcg
     4861 atctcgacat cggggaaggc gagtttctcg cgctcgtcgg gccctcgggg tgcggca
//
LOCUS       DASZMC010000402        11177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1298577,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000402 DASZMC010000000
VERSION     DASZMC010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298577"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VG274_08240"
     CDS             <1..391
                     /locus_tag="VG274_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWE06682.1"
                     /translation="LTGRDITVFGEGLQTRSFCYVDDLIDGFIKLMATPSDVIGPVNL
                     GNPDEFTIIDLAKAIIGLTGSASKIVHQSLPHDDPKQRQPDIAKAKELLGWTPTVPLT
                     EGLKRTIAYFDRLLSDRRAREVAGVPG"
     gene            553..1551
                     /gene="galE"
                     /locus_tag="VG274_08245"
     CDS             553..1551
                     /gene="galE"
                     /locus_tag="VG274_08245"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWE06683.1"
                     /translation="MNILVTGGAGYVGSHACCALRAAGYTPVTYDNLSRGFRGLVRFG
                     PFEQGDILDAPRLIEVFERHRPAAVMQFAALAHVGESVEKPLDYYRNNFDGALSLVAA
                     MRSCGVDKLVFSSTCATYGTPDGMPIAETCPQSPINPYGRSKLMVEQALQDASAAHGL
                     RSVSLRYFNACGAHPSGGIGELHRPETHLIPCVLMAASGEIDAIDIFGTDYPTADGTA
                     VRDYIHVCDLADAHVAALRYLEAGGETTALNLGTGRGHSVREIVDSATRVTGCAPASR
                     FAARRAGDPPMLVADATAANRVLNFTPGWTKIDRIVESAWAWHRSPARTALVAADS"
     gene            complement(1600..2133)
                     /locus_tag="VG274_08250"
     CDS             complement(1600..2133)
                     /locus_tag="VG274_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06684.1"
                     /translation="MKRKPQFIGTGLAALAGLLTMAATPTLAQYQNQPSNPQSQTAPN
                     SGQATPGNPGAPAVQASPSYQNSTGSMQNPGKTTSAASTESLSRVSHAKTTLASAQVQ
                     DSSGQQVGQVSNVHTTRKGHATKVDITLSSSNGGQPKTISVSASKLKYDKNSNTLITD
                     MTANDLQAMPAATSSGM"
     gene            complement(2430..3362)
                     /locus_tag="VG274_08255"
     CDS             complement(2430..3362)
                     /locus_tag="VG274_08255"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008307606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWE06685.1"
                     /translation="MKLLAGNANRPLAEAIAQHLKIPFTRAVVRRFADMEVFVEVQEN
                     VRGEDMFVLQSTSYPANDNLMELLVIIDALRRASARRITAVIPYFGYARQDRKVGPRT
                     PISAKLVANLITGAGANRVLTVDLHAGQIQGFFDIPTDNLYAQPVIVKDIQDYLGGRH
                     LMVISPDVGGVVRARALAKRLGADLAIVDKRRERAGESEVMNIIGDVRGRVCLLVDDI
                     VDSGGTMCNAADALVEQGAEEVYAYATHGVLSGGAVARIQSSRLKSVVVTDSIAATEE
                     VRKCPNIRRISIAPLLGEALRRINNEASVSSLFD"
     gene            complement(3439..4245)
                     /gene="pgeF"
                     /locus_tag="VG274_08260"
     CDS             complement(3439..4245)
                     /gene="pgeF"
                     /locus_tag="VG274_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWE06686.1"
                     /translation="MLKFSADNFGSASGIAHAFFGRSGGISMGLYASLNCGPGSNDDR
                     ARVIENRRVALAALGPSKSRLLTLYQVHGAETVEVREAWDLGRGPKADAMATSIRGVA
                     LGILTADCAPVLLADTEAGIVGAAHAGWKGALAGIVESVVNAMERLGARRGRIAAAIG
                     PCISQQAYEVGEELRARVIANDPADAQHFIRSDRDDHWRFDLPAYAKTRLEKAAVRST
                     SIIAACTYEREADFFSFRRATHRGETDYGRQLSAIMVAPDDPVYRGGIAC"
     gene            complement(4249..5325)
                     /locus_tag="VG274_08265"
     CDS             complement(4249..5325)
                     /locus_tag="VG274_08265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWE06687.1"
                     /translation="MNRLARRMARAIEMDGPLSIAAFATMALHDREDGFYASRESIGA
                     RGAFITAPEISQIFGELLGLWCVQAWRDQGSPTPARLVELGPGRGTLVKDALRAAHIA
                     PDFLARLEVVLVETSPALEAAQREQLRDAPAATRWVSQWDAIACDRPLFLLANEFFDA
                     LPIRQFVMTQKGWCERMVIVNAAGDLAFALAPISTPLDIPVERGAAEPGAVYETSPAA
                     QGLVEEASRAIAACGGGALFIDYGHEGCGFGDTLQAVSRHRQAGILDAPGESDLSAHV
                     DFAALARIASKAGAHAYGPIGQGEFLRTLGIGVRGARLAERNPACAEDIAMAIDRLTS
                     KQEMGALFKAFAISPGSAPPPPGF"
     gene            complement(5322..6206)
                     /gene="lgt"
                     /locus_tag="VG274_08270"
     CDS             complement(5322..6206)
                     /gene="lgt"
                     /locus_tag="VG274_08270"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWE06688.1"
                     /translation="MFEAVLPFPHIDPVLFRLGPLAIRWYALSYIAGLLAGWWYLLRL
                     LRTPSLWQSPTFGGRPPATADDIGDLVVWMTLGVIVGGRLGFVLIYGTFFCGIWGTSP
                     ACAGLPLAYLTNPIKIVAAWEGGMSFHGGLIGVTIALFLFARRRRLDLLKLADLVASV
                     TPLGLFFGRIANFINGELWGKATNVPWAMVFPRADQMPRHPSQLYEAGMEGLLLFAIM
                     QIGLRRFHLHDRPGLLAAIFCTFYGVFRYVAEIFRDSDTVFPSWLSVGQALSIPMWMV
                     AALLFWLAFRRPVAKAAG"
     gene            6317..6604
                     /locus_tag="VG274_08275"
     CDS             6317..6604
                     /locus_tag="VG274_08275"
                     /inference="COORDINATES: protein motif:HMM:NF016280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HWE06689.1"
                     /translation="MQTDNPLLDGLARLFTDAAGAAQSVRTEIDTFMRQRLERLVADM
                     DFVPREEFDAVKAMASKARSENERLEARIAVLEAQANPVAPPRAAPAPSKL"
     gene            6720..7220
                     /locus_tag="VG274_08280"
     CDS             6720..7220
                     /locus_tag="VG274_08280"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HWE06690.1"
                     /translation="MSLAQQEESVVEFHPLDMLEQSVEDHGWPFERSGRDELNLSVAG
                     RWCDHHFCFTWREDMQSLHLSSAFDMRVETARRSEMASLLMYINAKLWIGHFDLFSDE
                     GALVFRHSMIFPDARASAAQCEALLNLAVEACEHYYPAFQFVLWGGKTAEEAVAAAVL
                     ECAGQA"
     gene            7204..8046
                     /gene="proC"
                     /locus_tag="VG274_08285"
     CDS             7204..8046
                     /gene="proC"
                     /locus_tag="VG274_08285"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWE06691.1"
                     /translation="MPARPKRTACVTLLVGAGRMGSALLRGWVAQGIKPLAAVEPAPH
                     PDIEDFAQRHGIGLYRRLEDAAHLDVGACVVALKPQKLPTEARRLAPIAKSGALMLSI
                     AAGTGISALRRAWGREALIVRAMPNTPGAIGRGISALYAPKKVTADVRAHAQSLLAGL
                     GETLWVKREGLIDAVTAISGSGPAYVFLLVESLERAARAAGLPAESARRLARATISGS
                     GALLDSDARDAAALRRDVTSPGGTTEAALRVLMEDSALARLVARAVEAAHARAAQLRR
                     EGET"
     gene            8283..8882
                     /locus_tag="VG274_08290"
     CDS             8283..8882
                     /locus_tag="VG274_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06692.1"
                     /translation="MPKPDPQTRLADAAFRRLAEVSWADLTLASVARGAKVPLADLLE
                     SAPSKPALLGVMLRRLSGEVARRYRPDRESQSARDRVFDVCMTWFGLQRPRKTAMRAL
                     YNGLSRDPPALLSARREFVAAAEWILALAEADAGSALRIRAAIIAGLVARAIPVWLAD
                     DDEMGKTMAQLDRDLRRAENLLWPRGATTKKHRPTSRER"
     gene            9220..9684
                     /locus_tag="VG274_08295"
     CDS             9220..9684
                     /locus_tag="VG274_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06693.1"
                     /translation="MKITDYLVAGVSALAVSVSLSATASAHQIYYPYKHGVTARLDPN
                     RPLGHVGRYEPPYVARRHPSSGTWTDVSGKLPFMNGPWALQQLTDGTVLVQDFCTSQW
                     YKLTPDKKGQYYRRHLECDRGDAGELRAALLRHPDPAERADDRERGRIYRHR"
     gene            9590..10888
                     /locus_tag="VG274_08300"
     CDS             9590..10888
                     /locus_tag="VG274_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06694.1"
                     /translation="MPASYEPLFFATQILPNGQMIVNGGEYIGTDDICTEGSWSNQGA
                     LYDPVANSWTPVAPPVGWALLGDAESIILPNGSYMLADCCDDPAMQAIATITDGIVTW
                     TTGNSYTCPTGDFCNDEQSYTALPDGNVFMVDVWNYGANYDDYEIYDTTTGVWNLAGK
                     TADRMSDSTNFELGSAALTPQYGSRGTIIQFSGVSVSGMTPVNDVYNVASGAWRSGPV
                     LSYEGVDYTEADAPSATLPDGNIFMQASPGYGNTPSHFWEWSISGKGKVTATQVNDPK
                     QAPSTSDFESNMLVLPTGQVLWDNSQTTPNEVAIYTPVGTPKAAWRPAVSKVATTLTV
                     GSTGNAISGTQFNGFDLGGAYGDDAQAATNFPLVRIANINTGDVCFARSYNFSTMGVW
                     TAGATSAAFDIPATCETGASRLQVIVNGIASAAVRVKLKG"
BASE COUNT         2082 a         3586 c         3564 g         1945 t
ORIGIN      
        1 gctcaccggc cgcgacatca cggtcttcgg agagggcctg cagacgcgct cgttctgtta
       61 tgtcgacgat ttgatcgacg gcttcatcaa attgatggcc actccctccg acgtgatcgg
      121 acctgtcaac ctcggcaatc ccgacgaatt caccatcatc gatctcgcga aagccataat
      181 cggcctgacg ggatccgctt cgaagatcgt gcatcagtcc ttgccgcacg acgatccaaa
      241 acagcgacag ccggacatcg ccaaggcgaa ggaacttctc ggctggacgc cgacggtgcc
      301 gctcaccgag ggcctgaaac gcacgatcgc ctatttcgat cgtttgctgt ccgaccggcg
      361 cgcgcgcgaa gtcgccggcg tgcctggttg aggaaattat agccggtcat attggacggc
      421 atgtacagag gctcaccctt cgacaagctc agggtgagga aaataccctc atgccgagcc
      481 tgtcgaagca tgagcctgtc gaagcatgat tgaatcgaag tcaactcact gctggcgaaa
      541 gacggcccac aaatgaacat tctcgtgacc ggcggggcag gctatgtcgg cagccatgcc
      601 tgctgcgcgc ttcgcgcggc gggatatacg ccggtgacct atgacaatct ttcgcgcgga
      661 tttcgtgggc tcgtccgatt cggaccgttc gagcagggcg atatcctcga tgcgccccgc
      721 ctcatcgaag tgttcgaacg ccaccggccg gcggctgtga tgcaatttgc ggcgctcgcc
      781 catgttggcg aatccgtcga gaagccgctc gactactacc gcaacaattt tgacggcgcg
      841 ctttcgctcg tcgcggcgat gcgcagttgc ggcgtcgaca aactggtctt ttcgagcacc
      901 tgtgcgacct atggaacgcc ggacgggatg ccgatcgccg agacgtgtcc gcagtcgccg
      961 atcaatccct acggccgctc caaattgatg gtggaacagg cgctccagga tgcaagcgcc
     1021 gcgcacggac tgcgatccgt ctcattgcgc tatttcaatg cctgcggcgc gcacccgtcc
     1081 ggcgggatcg gcgaactgca tcggcccgaa acccatctga tcccgtgcgt cctgatggcg
     1141 gcgagcgggg agatcgatgc gatcgacata ttcggcaccg attatccaac cgcggacggc
     1201 accgcggtgc gcgactacat tcatgtctgc gatcttgccg acgcccatgt cgcggccttg
     1261 cgctacctcg aagcgggcgg cgaaacgacg gcgctcaatc ttggaaccgg ccgcggtcat
     1321 tcggtgcgcg agatcgtcga ttcggcaacc cgcgtcacgg gctgcgcgcc ggccagccgt
     1381 ttcgcggcga gaagggcggg ggacccgccg atgctggtgg cggatgcaac ggccgccaat
     1441 cgcgttttga acttcacgcc cggatggacg aaaatcgacc gcatcgtcga atcggcctgg
     1501 gcctggcatc gaagccccgc gcgcacggcc cttgttgcgg ctgacagctg acgggccggc
     1561 gatatcccgc cggcccgcct ctcgatccgt cctgctgttc tacatgcccg aagatgtcgc
     1621 cgccggcatg gcctgaaggt cgttggcggt catgtccgtg atcagcgtgt tgctgttctt
     1681 gtcatatttc agcttgctgg cgctgacgga aatcgtcttc ggctggccgc cattcgacga
     1741 actgagggta atgtcgacct tcgtcgcgtg tcccttgcgc gtcgtgtgga cattgctgac
     1801 ctggccgacc tgctgaccgc tcgaatcctg cacttgcgcg gaagccagcg tggtcttcgc
     1861 atggctgacc cggctcagcg attcggtgct cgcggcagac gtcgtcttgc cgggattctg
     1921 catcgagccg gtgctgttct ggtatgacgg gctcgcctga acggcaggcg ctccgggatt
     1981 gccgggcgtg gcctggccgg aattaggcgc ggtctggctt tgtggattgg aaggctggtt
     2041 ctgatactgc gccaaagtcg gcgtcgccgc catcgtcaaa aggccggcga gcgccgccag
     2101 gccggtgccg ataaattgtg gtttgcgttt catggtgcaa ctcctgtttg cggttggaga
     2161 gggaatccgg tgcgtggacc cgacactccg caaacgtccg gcggaatggc gcgttccggc
     2221 gccggcgacg cgcgagcccg ctaacagaag tcagagcacg cgcgacaggc atggttcgat
     2281 ctgcctgtgc gaaagctgaa gtttcgctct cgccgcggct cccgcgcgcg gcgggtcggc
     2341 gaaggcaggc aagctcacca tgacggactc atcccaagcc tgtcgaagag cgcctcatcc
     2401 tgagcctgtg gaaggatgat taccggatgt taatcgaaca gactcgagac gctggcttcg
     2461 ttgttgatgc ggcgcagggc ttcgccgaga agcggagcga tggagatgcg ccggatgttc
     2521 ggacatttgc gcacttcctc ggtcgcggcg atggagtcgg tcacgacgac cgatttgagc
     2581 cgggacgact ggatgcgcgc gactgcgccg cccgacagca cgccgtgtgt cgcataggca
     2641 tagacttctt cggcgccctg ttcgacgagc gcatccgcgg cgttgcacat cgtgccgccg
     2701 gaatcgacga tgtcgtcgac cagaagacag acgcgcccgc gcacgtcgcc gatgatgttc
     2761 atcacttcgg actcgccggc gcgctcgcgc cgtttgtcga cgatggccag atccgccccg
     2821 aggcgcttgg cgagcgcacg cgcccgcacc acgccaccga catcgggcga gatcaccatg
     2881 agatggcgtc cccccagata atcctggatg tccttcacga tcaccggctg cgcgtagaga
     2941 ttgtcggtcg ggatatcgaa gaagccctgg atctggccgg cgtggagatc cacggtgagc
     3001 acgcggttgg cgccggcgcc ggtgatgaga ttggcgacca gcttggcgga aatcggcgtg
     3061 cgcggaccga ctttgcggtc ctgccgtgca tagccaaaat agggaatgac ggcggtgatg
     3121 cgccgcgcgc tggcccggcg cagggcgtcg atgatgacga ggagctccat cagattgtcg
     3181 ttggcgggat agctcgtcga ctgaaggacg aacatgtcct ccccgcgtac gttttcctgc
     3241 acctcgacga acacttccat gtcggcgaag cggcgcacca cggccctggt gaagggaatc
     3301 ttcagatgct gggcgatggc ctccgcgagc gggcgattgg cgttgcccgc tagcagcttc
     3361 atcccccgcg ctccgttcgc gtcgctcata aaacgctccg gtcccgcttg tgaaactttg
     3421 gtgaacccct cgcgacttct agcaagcgat tccgccgcgg taaaccggat cgtccggcgc
     3481 caccatgatg gcggagagct ggcggccata atcggtttct ccacgatggg tggcgcggcg
     3541 gaagctgaag aaatcggctt cccgctcata ggtgcaggcg gcgatgatgg aagtgctccg
     3601 caccgccgcc ttttcgaggc gcgttttcgc ataggcggga agatcgaagc gccaatgatc
     3661 atcgcggtcg gagcgaatga agtgctgcgc gtcggcggga tcgtttgcga tcacgcgagc
     3721 gcgaagctcc tcgccgactt cataggcctg ttgcgaaata cacggcccga tcgctgccgc
     3781 gatccgcccg cgccgcgcgc cgaggcgttc cattgcgttc actaccgact cgacgatccc
     3841 cgcgagcgcg cccttccaac ccgcatgggc ggcgcccaca attccggcct cggtgtccgc
     3901 gagaagcacc ggcgcgcaat cggcggtgag aatcccaagc gcgacgcccc gaatcgacgt
     3961 cgccatcgca tccgcctttg gcccacggcc aaggtcccac gcttcgcgca cctccacggt
     4021 ttcagcgcca tgcacctggt aaagggtgag caatcggctc ttcgatggtc cgagcgccgc
     4081 aagcgccacg cgccgattct cgatcacacg ggcgcggtcg tcgttggaac ccgggccgca
     4141 attgagcgac gcgtaaagcc ccatcgaaat gccaccggac cgcccgaaga aggcgtgcgc
     4201 aattccgctc gcgctgccaa aattgtcggc cgagaatttc agcatcggtc aaaatcccgg
     4261 tggaggtgga gcgctgcctg gcgagatcgc aaatgccttg aacagcgcgc ccatctcctg
     4321 cttgctggtg agccggtcga tggccatggc gatgtcctcg gcgcaggccg gattgcgttc
     4381 cgcgagcctg gcgcctcgca ccccgatccc gagcgtacgc agaaactcgc cctgcccaat
     4441 gggaccgtaa gcgtgtgcgc cggccttgct cgcaatgcgc gcgagcgccg cgaaatcgac
     4501 atgggccgag agatccgatt ctcccggcgc gtcgagtatg ccggcctgcc tgtgccggga
     4561 cacggcctgc aaggtgtcgc cgaagccgca accctcatga ccgtagtcga tgaagagcgc
     4621 gcccccgcca caagcggcga tggcgcggct tgcttcttcc accagaccct gcgccgccgg
     4681 tgaagtttca tataccgcgc ccggttccgc ggcgccgcgc tccaccggta tgtcgagcgg
     4741 cgtggaaatt ggagccagcg cgaaggcgag atcgcccgca gcgttcacga tcaccatccg
     4801 ctcgcaccac cccttctgcg tcatgacgaa ttggcgaatg ggcagcgcgt cgaagaattc
     4861 attggcaagc aggaacaatg gccggtcgca agcgatcgca tcccattgcg acacccagcg
     4921 tgtagcagcg ggcgcatcgc gcaattgttc gcgctgcgcg gcttcaagcg ccggacttgt
     4981 ttccaccaga acgacctcga gccgggccag gaaatcgggc gcaatatgcg ctgctcgcaa
     5041 tgcgtccttc accagcgttc cgcggccggg acccagttcc acaaggcgtg ccggggtcgg
     5101 actgccctga tcccgccagg cctggacaca ccacaagccg agaagctctc caaaaatctg
     5161 gctgatctcc ggcgcggtga tgaaggcccc gcgcgcaccg atcgattcgc gcgacgcata
     5221 gaatccatct tcgcgatcgt gcaacgccat cgtcgcgaac gcggcaatcg aaagcgggcc
     5281 gtccatttcg atcgcccgcg ccattcgtcg cgccagccgg ttcatcccgc ggctttcgcg
     5341 accggccggc gaaaggccag ccagaagaga agcgccgcca ccatccacat cggaatgctg
     5401 agcgcctgcc cgacgctcag ccagctcggg aaaaccgtgt ccgaatcgcg gaagatttcg
     5461 gcgacgtagc ggaagactcc gtaaaacgtg cagaagatcg cggcgagcag gcccgggcgg
     5521 tcatgcaggt ggaaccgcct cagcccgatt tgcatgatgg cgaacaggag cagtccctcc
     5581 atgccggcct catacagctg gctcggatgg cgcggcattt gatcggcgcg cgggaatacc
     5641 atcgcccatg gaacgttggt agccttgccc cacaactcac cgttgatgaa attagcgata
     5701 cgtccaaaaa atagcccgag cggcgtaacg ctggcgacga gatcggcgag tttcagcaaa
     5761 tcgagtctgc ggcggcgggc gaagagaaaa agcgcgatgg tcacgccaat gaggccgcca
     5821 tgaaacgaca tgccgccttc ccaggcggcg acgatcttga tcggattggt gagataggcg
     5881 agcggaagtc cggcgcatgc cggcgaggtg ccccagatgc cgcagaagaa cgtgccgtag
     5941 atcagcacga agccgaggcg cccgcccacg atgacgccga gcgtcatcca cacgaccaga
     6001 tcgccgatgt cgtcggcggt tgcgggcggg cgtccgccga aagtcgggct ttgccaaagc
     6061 gacggcgtcc gaagaaggcg caggagatac caccagccgg caagcagacc tgcgatgtag
     6121 gaaagcgcat accagcggat cgccagcggc cccaggcgaa agagcaccgg atcgatgtgc
     6181 ggaaagggaa gaacggcttc gaacatcggg ccttgaagaa ggcaggcgcg gtaagacgct
     6241 ctgcttcgcc tttggctgtc aaggatgaat cccggcagcg cttgccctcg ggcggccggt
     6301 cccataagga gcagccatgc agacggacaa tcctttgctc gacggcctgg cgcggctgtt
     6361 caccgacgcg gccggcgccg cacagagcgt gcggaccgag atcgacacct tcatgcgtca
     6421 gcgtctcgaa cgcctcgtcg cggacatgga tttcgtgccg cgcgaagagt ttgacgccgt
     6481 gaaagcgatg gcatcgaagg cgcgcagcga gaacgagcgg ctcgaagcgc ggatcgccgt
     6541 cctcgaggcg caggcgaatc cggtcgcccc gccgcgcgcc gcgccggcgc cgtcaaagct
     6601 gtgaatggag cgaagacccc aaactggttg agggtcggac tcttgtcgaa ccacaaaatc
     6661 ttgtgtcagg ttggctgcaa gctgcttcgt tttgccgacc accatccagg agttcgcgga
     6721 tgagcttagc ccagcaggaa gaatcggtcg tcgagttcca tccgctcgac atgctcgagc
     6781 agtcggtgga agaccatggc tggcctttcg agcggagcgg gcgcgacgag ctgaacctct
     6841 cggtggccgg gcgctggtgc gaccatcatt tctgcttcac ctggcgggaa gacatgcagt
     6901 cactccatct ttcctccgcc ttcgacatgc gggtcgaaac cgcgcggcgc agcgagatgg
     6961 cctcgctgct catgtacatc aacgccaagc tgtggatcgg acatttcgat ctgttctccg
     7021 acgagggtgc gctggtcttc cgccattcga tgatttttcc cgacgcgcgc gccagcgccg
     7081 cccaatgcga agcccttctc aatctcgcgg tggaagcctg cgaacactac tatccggcgt
     7141 tccagttcgt cctctggggc ggcaagacgg ctgaggaagc cgtggcggcc gccgttctcg
     7201 agtgtgccgg ccaggcctaa gcgcaccgct tgcgtcacgc tgctcgtggg cgccggccgc
     7261 atgggcagcg cgcttctcag gggctgggtc gctcagggca taaagccgct tgcggcggtt
     7321 gagcccgcac cgcatcccga catcgaagat ttcgcgcagc ggcatggcat cggcctttac
     7381 cgcaggctcg aggacgcggc gcatttggat gtcggcgcct gtgtggtcgc gctcaagccg
     7441 cagaagctgc ccaccgaggc gcgccgcctt gcgcccatcg caaagtccgg cgctctcatg
     7501 ctgtcgatcg cggcaggcac cggcatttcc gcgcttcgcc gggcctgggg cagggaggcc
     7561 ctgatcgtcc gcgcgatgcc gaacacgccg ggcgcgatcg gccgcggcat ttccgccttg
     7621 tacgctccga aaaaagtgac cgccgatgtg cgtgcgcatg cgcaatctct tcttgccggg
     7681 ctgggcgaaa cattatgggt gaagcgcgag gggctcatcg acgccgtgac cgcaatatca
     7741 ggctcgggcc cggcctatgt gttccttctc gtggagagtc tggaacgcgc ggcgcgcgcc
     7801 gcgggcctgc ccgcggaaag cgcgcgccgt ctcgcgcggg cgacgatctc cgggtccggc
     7861 gctctgctcg attcggatgc gcgggatgcg gcagcgctgc gccgcgacgt gacaagtccg
     7921 ggcggcacga ccgaagccgc cctccgcgtg ctgatggagg actccgcact cgcgcgactt
     7981 gtcgcaagag cggtcgaggc ggcgcacgct cgggcagcgc agctgcgccg cgaaggcgag
     8041 acatagccgt ttgacgcagt gcttcgattc cgaaactcgc cacacaaaaa attgtcatgg
     8101 ccctacttga tggggccacc cagccagcgc gcgtccgcgc gctgaattga tgctctgagc
     8161 ggtttcatct gggtggcccg ctcggagttt acattccggc cgcgcttcgt gcggaccggg
     8221 gtggcgggcc atgacaattt tttgcaattc aattctcagc aatggaccag taggatcgcg
     8281 tgatgcccaa accggacccg caaacccgtc tcgccgacgc agctttcaga cggctcgccg
     8341 aagtcagctg ggccgatctc accctcgctt ccgtcgcgcg cggcgcaaaa gttcccctgg
     8401 ccgaccttct tgaatccgcg ccttcaaaac ccgcgctcct gggcgtcatg ctgcgccggc
     8461 tttccggcga agtggcgcgg cgttaccggc ccgaccgcga atcgcagagc gcgcgcgacc
     8521 gcgtcttcga tgtgtgcatg acatggttcg gattgcagag gccccgcaaa acggcgatgc
     8581 gcgcgcttta caacggcctc agccgggatc cgccggcgct tttaagcgcg cgccgcgaat
     8641 tcgtcgccgc cgcggaatgg attctcgccc tcgccgaagc ggacgcaggt tccgcgctgc
     8701 gaattcgggc ggcgatcatt gccggcctcg tcgcgcgcgc gatcccggtc tggcttgccg
     8761 acgacgacga gatgggaaaa acaatggcgc agctcgaccg cgatctccgc cgggctgaaa
     8821 atctgctctg gccgcgcggc gcaacaacaa agaaacatcg tccgacatcg cgcgagcgct
     8881 gacagttcgg cgaggcgatg ttatgcccgc ggcctcaagc ttcaaccggt ccggcacggg
     8941 gaaaattact gaaaatacgg catacgatcg gcgatgctct gcccgatcgt gcgccggccg
     9001 gcgcctgtgg cggcgtcggg tgcataggat aatgcgatag cgccgatacc gtgtgggtcg
     9061 gacagatttc aaacgcgcgt cgcaaatggt gacgcaagcg tcgagaattt ccgtgcgcgg
     9121 agcgccggga aacgttcggc cgattgattt gttcctctcc catcaaagga caaccggcgc
     9181 tgaaaccggc gcggggccgg cccagaggga gatactttga tgaaaataac tgattatctg
     9241 gtcgccggcg tttcggctct ggcagtgtcg gtgtcgctct ccgccacagc ctctgcgcac
     9301 cagatttact acccttacaa acacggcgtc accgcgcgcc tcgatcccaa ccggccgctt
     9361 ggtcatgtgg ggcgctacga gccgccctat gtggccagga ggcacccgag ctccggcacg
     9421 tggacggacg tgagcggaaa gcttcctttc atgaatggcc cctgggcgct gcagcagctc
     9481 acggatggca cggttcttgt ccaggatttc tgcacgtcgc aatggtacaa gctgaccccc
     9541 gacaaaaagg gccaatatta ccgacggcac ctggagtgcg atcgcggcga tgccggcgag
     9601 ctacgagccg ctcttcttcg ccacccagat cctgccgaac gggcagatga tcgtgaacgg
     9661 gggcgaatat atcggcaccg atgacatctg cacggaaggc tcctggtcga accagggcgc
     9721 gctttatgat ccggtagcca acagctggac acccgtcgcg ccccccgtgg gctgggcgct
     9781 gctcggcgac gcggagagca tcatcctgcc gaatggaagc tacatgctcg ccgattgctg
     9841 cgacgatccg gcgatgcagg cgatcgcgac gatcaccgac gggatcgtga cctggaccac
     9901 gggcaacagc tacacctgtc cgaccggcga tttctgcaac gacgaacaga gctataccgc
     9961 actgccggac ggcaacgtgt tcatggtgga cgtctggaat tacggcgcca actatgacga
    10021 ctatgagatc tacgatacga cgacgggtgt ctggaatctt gccggaaaaa cggcggatcg
    10081 catgagcgat tcaaccaatt tcgaactggg ttctgcagcg ctgaccccgc aatacggttc
    10141 gcggggaacc atcatccagt tcagcggcgt ttcggtttcc gggatgacgc ccgtcaatga
    10201 tgtctataac gtcgcaagcg gcgcctggag aagcggtccc gtactgtcgt acgagggggt
    10261 ggattacacc gaagcggatg cgccgtcggc gacgctgccc gatggaaaca tcttcatgca
    10321 ggcaagcccc ggctacggca acacgccctc gcatttctgg gagtggagca tcagcgggaa
    10381 gggcaaggtg acggcgacgc aggtgaacga cccgaagcag gcgccgagca ccagcgactt
    10441 cgaaagcaac atgctcgttc tgccgacggg gcaggttctc tgggataaca gccagacgac
    10501 gccgaacgag gttgcgatct acacgccggt gggcacaccc aaagcggcat ggcgtccggc
    10561 ggtctcgaag gtggcgacca cacttacggt cggcagcacg ggcaacgcga tctcgggcac
    10621 ccaattcaac ggcttcgatc tgggcggcgc atatggtgac gacgcgcaag ccgccaccaa
    10681 cttcccgctc gtacgcatcg ccaacatcaa cacgggcgac gtctgcttcg cgcgcagcta
    10741 caacttctcg accatgggtg tgtggactgc cggcgccacg agtgctgcgt tcgacatccc
    10801 ggccacctgc gagaccggcg cgagcaggct tcaggtcatc gtcaacggca tcgcctcggc
    10861 cgccgtccgc gtgaagctga aaggttaggg cgcgcgacag cgcgttcgcg tgaatgcgcg
    10921 gcatgaccat gggcggggaa acccgcccat gtttttgtaa aaaccgctgg ccttaggacg
    10981 caccggacag gattcgaacc atcgaatctg gcggatgaaa caaatacgtg gcccaagctc
    11041 cacgtttcct cccccaacgt gggggaggtg gcccgcaccg gtccgcgcga cgcgcggccg
    11101 gaggacaagc tccgggccgg aggggaacct caccgcacgc gacgtccccc tccgcctcgc
    11161 tgcgctcggc acctccc
//
LOCUS       DASZMC010000403         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2923249,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000403 DASZMC010000000
VERSION     DASZMC010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2923249"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VG274_08305"
     CDS             complement(<1..463)
                     /locus_tag="VG274_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWE06695.1"
                     /translation="MLIEEKDFGTPARRSERNVTLTIDGQRAIVPAGTSILRAAAGCG
                     TDIPKLCSTDSLEAFGSCRLCLVEIEGRPGTPASCTTPVADGMIVRTQTERLARIRRG
                     VMELYISDHPLDCLTCAANGDCELQDMAGAVGLREVRYGYAGENHLDDAKDQ"
     gene            complement(862..>2369)
                     /locus_tag="VG274_08310"
     CDS             complement(862..>2369)
                     /locus_tag="VG274_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HWE06696.1"
                     /translation="GAERVATAIAEAAKRATLEVELVRTGSRGLYWLEPMVEVVTRNG
                     RVAYGPLRSDDVPGLFACGFLDGGRHLRALGQTDSIPWLKRQTRLTFARCGMTDPLSL
                     DDYRAHDGYKGLMRALTLKPQEVVEEVARSGLRGRGGAGFPTGIKWRTAAEAKGDTKY
                     IVCNADEGDSGTFADRMLMEGDPFLLIEGMTIAGLATGATKGVIYCRSEYPHAIATTS
                     AAIATARAANLLGARIADSNRDFDIELRAGAGSYVCGEETALLESLEGKRGQVRAKPP
                     LPAHAGLFAKPTVVNNVLTFAAAAWVLAEGADAYKALGYGRSRGTMPIQLAGNLKHPG
                     LFEAPFGMTLGELIDDVGGGTASGRPVRAVQAGGPLGAYFPRAMFDMPYDYEAYTAND
                     GLIGHGGLVVFDDTVDMARQARFAMEFCALESCGKCTPCRVGSIRGVEVVDRIVAGID
                     REANAALLKELCETLKFGSLCALGGFTPYPVMSALTHFPEDFGLARVAEKV"
BASE COUNT          418 a          774 c          757 g          420 t
ORIGIN      
        1 actggtcctt cgcgtcgtcg agatggttct cgcccgcata gccgtagcgc acctcgcgca
       61 gtccgactgc gcccgccatg tcctgcagtt cgcaatcgcc attcgccgcg caggtgaggc
      121 agtcgagcgg atggtcggat atgtagagct ccatcacgcc gcggcggatg cgggcgagcc
      181 gttccgtctg tgtacggacg atcatgccgt ccgcgaccgg cgtggtgcag gaggcaggcg
      241 tgccgggccg gccttcgatc tcgacgaggc acagccggca ggagccgaaa gcctcgagcg
      301 aatcggtcga gcagagtttg ggaatgtcgg tgccgcaccc ggccgcggcg cgcagaatcg
      361 aggtgccggc gggcacaatc gcccgctggc cgtcgatggt cagggtgacg ttgcgttcgc
      421 tccgcctcgc cggcgtgccg aaatcctttt cctcaatcag catgttcaac aacactatct
      481 cttcaatgtc atccccgcga aagcgctagg gcatctacac atttcggcga tggcgatcga
      541 gacttcagcc aacctccccc ttgtggggag gtcgaaaaac gcccttcgcg tttttcgggt
      601 gggggtcgct gcctatccat ggagagccga gacccccacc cgaaatttgt tcgcgcgaaa
      661 agcgctcgca aatttcgacc tctcctcaag ggggaggtcg aatgaaatcg ctgtcgatgc
      721 gcctacaaca gccaccgaga tgtgtagatg ccatagcgcg aaagcgggaa cccaggggca
      781 agcgacgtgc gttcgctccg gattcccgct cctgcgggaa tgacatgttt ggaatttgga
      841 caaacggaac actccgtcat cctacacctt ctctgccacc cgcgcgagcc cgaaatcctc
      901 gggaaaatgg gtcagcgcgc tcatcaccgg atagggcgtg aagccgccca gcgcgcagag
      961 cgagccgaat ttcagcgtct cgcaaagttc tttcaaaagt gctgcattcg cctcgcggtc
     1021 gatgccggca acgatacggt cgacgacttc gacgccccgg atcgagccga cgcggcaggg
     1081 cgtgcacttg ccgcaggatt ccagcgcgca gaactccatc gcaaagcgtg cctggcgcgc
     1141 catgtcgacc gtgtcgtcga acacgacgag cccgccatgg ccgatcaggc cgtcattggc
     1201 ggtgtaggcc tcgtaatcgt atggcatgtc gaacatggcg cgcgggaaat aggcgccgag
     1261 cgggccgccc gcctgcaccg cacgcaccgg ccgtccgctc gcggtgccgc cgcccacatc
     1321 gtcgatcagt tcgcccagcg tcatcccgaa cggcgcctcg aagagaccgg gatgtttgag
     1381 attgcccgcg agctggatcg gcatggtgcc gcgcgaacgg ccgtagccga gcgccttgta
     1441 ggcatctgcg ccttcggcga gcacccacgc cgccgcggcg aaggtcagca cattgttcac
     1501 cacggtcggc ttggcaaaca gtccggcatg ggcgggcagg ggcggtttcg cgcgcacctg
     1561 gccgcgtttg ccctcgaggc tttcaagcaa agcggtttct tcgccgcaga cgtaggaacc
     1621 tgcgccggca cgcagctcga tgtcgaaatc gcgattcgaa tctgcaatgc gcgcgcccag
     1681 aagattcgcc gcgcgcgcgg tcgcgatggc ggcactcgtc gtcgcgatgg cgtggggata
     1741 ttccgagcgg caatagatca cgcctttcgt cgcgccggtg gcgaggcccg caatcgtcat
     1801 gccttcgatg agcaggaagg gatcgccctc catcagcatg cggtcggcga aggtgccgct
     1861 gtcgccttcg tcggcattgc agacgatgta tttggtgtcg cctttggctt ccgccgcggt
     1921 acgccacttg atgccggtgg gaaatcccgc gccgccgcgg ccgcgcaggc ccgaccgcgc
     1981 aacctcttcc acaacctcct gcggcttgag agtcagtgcg cgcatgagcc ccttgtagcc
     2041 gtcatgcgcg cggtaatcgt cgagcgacag cggatcggtc atgccgcaac gcgcaaaggt
     2101 gagtcgcgtc tggcgtttga gccatgggat ggaatccgtt tggccgagcg cgcgcaggtg
     2161 ccttccgccg tccagaaatc cgcaggcgaa gagtccgggt acgtcgtcgg acctgagcgg
     2221 accatacgcc acgcgcccgt tccgcgttac gacttcgacc atcggctcga gccaatagag
     2281 tccgcgcgag cccgtgcgca cgagttcgac ttcgagcgtg gcgcgttttg ctgcctcggc
     2341 aatggccgtc gcaacgcgct ccgcgccga
//
LOCUS       DASZMC010000404         6584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3198368,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000404 DASZMC010000000
VERSION     DASZMC010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3198368"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VG274_08315"
     CDS             complement(<1..393)
                     /locus_tag="VG274_08315"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06697.1"
                     /translation="MTVFFWKPSALAALNRCGAWIAGARGWRRFVLALGAGLVSALAF
                     APIGFFPALLVACAVLVLLIDGAVPRANPLRVAALTGWSFGFGQFLAGLYWVGYAFTV
                     DASAHAWQIPFVAVLLPGGLALFPALACG"
     gene            complement(528..1460)
                     /locus_tag="VG274_08320"
     CDS             complement(528..1460)
                     /locus_tag="VG274_08320"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWE06698.1"
                     /translation="MSDQNRKDHPATGNGRSASLLQRLSHLLRRGHEMHAIRESLEEV
                     IEESDRQSAELSPQERIMLANLLKFGELRVGDVMVPRADIVAVEENTSLKELVTGVRE
                     GQHSRLPVYRETLDDPIGLVHIKDVLSLVEIAEDGGMRLPDVSIAQIRRNLLFVPASM
                     PALDLMLKMQTTHMHLALVIDEYGGTDGLVSIEDLVEEIVGDIDDEHDVAEPPQLKAL
                     PDGGYEADARLDLDDFREQTGFDIEPEGEDEDVDTLGGFVVSLLGRVPERGEIIPHMS
                     GLEFEVLEADPRRVKRLRLRKSQPAPDLHPERET"
     gene            complement(1479..1946)
                     /gene="ybeY"
                     /locus_tag="VG274_08325"
     CDS             complement(1479..1946)
                     /gene="ybeY"
                     /locus_tag="VG274_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWE06699.1"
                     /translation="MTHSISVVVEEPGWRRRGVGLPLLRNAARLALVRGAHNQNAAQL
                     TLLLTHDERLRALNAQFRGIDAPTNVLAFPAAAAKAYLGDVALALGVAEREATRAGIG
                     LCAHALHLTVHGVLHLLGYDHVSVREARIMECLETAILHELGLASPYAPIAAE"
     gene            complement(1943..2920)
                     /locus_tag="VG274_08330"
     CDS             complement(1943..2920)
                     /locus_tag="VG274_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWE06700.1"
                     /translation="MTLEFPDNQKLAALGGAHQKHLVRVEQKLGVRVATRGNLISVEG
                     APESRERAAGVLRALYARLEGGEEVALADVDAEIRFAMHEPETPGAAEFGTGSIKTAA
                     SRVARARSPAQRAYLDLMRTHPLVFSIGPAGTGKTYLAAAYGAHLLHERRVERLILSR
                     PALEAGERLGFLPGDLKEKIDPYLRPLYDALFETMGELCVRLMESGVIEVAPLAFMRG
                     RTLARAFVILDEAQNCTSAQMKMFLTRLGEDARMVVTGDPSQSDLPGGRPEGLNEALA
                     TLAHVEGVATAHFSDTDVVRHPLVTRIVRAYQDKGAAARDVRRSRATEE"
     gene            complement(2962..3411)
                     /locus_tag="VG274_08335"
     CDS             complement(2962..3411)
                     /locus_tag="VG274_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HWE06701.1"
                     /translation="MSSDFIVGFPGETDADFDATLQLVRDVRYAQAFSFKYSARPGTP
                     AAALPKQIPEDIKSTRLAQLQALLAAQQAEFNQLCAGSTMSILFEKPGTKPGQAIGRS
                     PWLQPVHVENARNLIGRIRDVRIAQVLSNSLKGELVAQNANAVAGSC"
     gene            complement(3451..4323)
                     /locus_tag="VG274_08340"
     CDS             complement(3451..4323)
                     /locus_tag="VG274_08340"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HWE06702.1"
                     /translation="MKKLFVKTFGCQMNVYDSARMADLLAAIGYEPDDRPEDADLVIL
                     NTCHIREKAAEKMYSELGRLKKLKDSRARAGRPMLIAVAGCVAQAEGEEIIARQKAVD
                     MVVGPQSYHRLPEMIARISRGAGHVLETEFPAQEKFDSLPEERSDCGPSAFLTVQEGC
                     DRFCTFCVVPYTRGAEFSRPPERIADEARQLAEKGVKEVTLLGQNVNAYRGIAADGKA
                     WSLARLIASLAEIEGIERLRYTTSHPRDMSDDLIAAHREVVKLMPYLHLPVQSGSDRV
                     LAAMNRQHTAERYL"
     gene            complement(4387..5250)
                     /locus_tag="VG274_08345"
     CDS             complement(4387..5250)
                     /locus_tag="VG274_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWE06703.1"
                     /translation="MQSLRAAAILSVFLLVTIVLIPWQEMAVRLRLKRRKTFPQRYHR
                     FLCRLFGIRVTVVGRPVCSSGVLMVANHTSYFDILVLSSAARVSFVAKSEVANWPLFG
                     TLARLQETVFIERAKRSQTVEARDLIRERLAQGDALVLFPEGTSNDGNRVLSFKSALM
                     GAAEAELGTDAQGRARHVPVQPVSVSYVGLYGLPMARDMRPLFAWYGDMELVPHLWEA
                     MQTGPLDVVVEFHEATSVDIEGSRKALAVVAEAAVRRGQARALAGLPPDARAQLRTTA
                     PRQESRAAAAA"
     gene            complement(5252..5704)
                     /locus_tag="VG274_08350"
     CDS             complement(5252..5704)
                     /locus_tag="VG274_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWE06704.1"
                     /translation="MPRLCGRDGRLSRIEKLCAEKGLRMTDQRRVIARVLSNAEDHPD
                     AEELYRRAAAIDPHISIATVYRTVKLFEDAGILERHDFRDGRSRYEEVPDSHHDHLID
                     VQSGRVIEFRNEEIEKLQRRVAEELGFELIDHRLELYGVPKGARKRGH"
     gene            complement(6166..>6584)
                     /locus_tag="VG274_08355"
     CDS             complement(6166..>6584)
                     /locus_tag="VG274_08355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE06705.1"
                     /translation="VWGPEFLGRLLAQPGAFSLVALERGEPAGFVVARAVVGEAEILS
                     LGVRHESRRQGLGSGLIRMATARAAAMGAAQICLEVAVENDAARALYGALGFREVGAR
                     PAYYHGTGGVRGDGLILKRALINSGENPAIMSWRAE"
BASE COUNT         1243 a         2101 c         2053 g         1187 t
ORIGIN      
        1 tccgcaggcc agcgccggaa aaagcgccag tcccccgggg agcaggacgg ccacgaacgg
       61 aatttgccag gcatgcgcgg atgcgtcgac cgtgaaggcg tagccgaccc agtagagacc
      121 ggcgagaaac tgcccgaagc caaaggacca gcctgtgagc gccgccacac gaagcggatt
      181 tgcgcgaggc acggcgccat cgatcagcag cacaaggacg gcgcacgcca caaggagcgc
      241 aggaaagaat ccgatcggtg caaaagccag cgccgacacg agacccgcgc cgagcgccag
      301 aacaaatctg cgccagccgc gcgcgcccgc gatccatgcg ccgcaccggt tgagcgccgc
      361 caacgcgcta ggcttccaaa aaaaaactgt catggccccg ctcgacgggg ccacccagcc
      421 agcgcgcgtc cgcgcgctga atgaacgctc tgagcggttt cgcctgggtg gcccgctcta
      481 cggcggacca tgacaatatt ttggtacgaa gtgttggact cggacaatta cgtctcgcgc
      541 tcgggatgca gatccggcgc tggctgcgac ttgcgcaggc gcaggcgctt gacccggcgc
      601 gggtctgcct cgagcacttc gaactcgaga ccggacatgt gagggatgat ctcgccgcgt
      661 tcgggaaccc ggccgagaag cgacacgacg aagcctccca gcgtgtcgac atcctcgtct
      721 tcgccctccg gctcgatatc gaagcccgtc tgttcgcgaa aatcgtcaag atcgagccgc
      781 gcatcggctt cgtaaccgcc gtcgggcaga gccttcagct gcggcggctc agcgacgtca
      841 tgttcatcat cgatgtcgcc gacgatctcc tccaccagat cttcgatgga gaccaggccg
      901 tccgttcctc catattcgtc gatgaccagc gccagatgca tatgcgtcgt ctgcatcttg
      961 agcatcagat ccagagcggg catggacgcc gggacaaaga gaaggttgcg ccggatttgt
     1021 gcgatcgaga catcgggcag gcgcatgccg ccgtcttccg caatttcgac cagcgaaaga
     1081 acgtccttga tgtgcacgag gccgatggga tcgtcgagcg tctcgcgata gaccggcagg
     1141 cgcgaatgct gcccttcgcg aacgccagtg acgagttcct taagggaggt attctcttcg
     1201 acggctacga tgtcggcgcg cggaaccatc acgtcgccga cgcgaagctc gccgaacttg
     1261 agcaggttgg cgagcatgat acgttcctgc ggcgagagct cggcactttg acgatcgctt
     1321 tcctcgatga cttcttcgag actctcccgg attgcgtgca tctcgtggcc gcgtcgcaaa
     1381 agatgcgaaa gccgctgcag cagcgacgcg ctccgtccgt tgccggtagc aggatggtcc
     1441 ttgcggtttt gatccgacat gggtctggaa tttcgtaact attcggccgc aatgggcgcg
     1501 taggggctcg caagcccaag ctcgtgcagg attgcagtct ccaggcactc catgatcctg
     1561 gcctcgcgaa ccgagacgtg atcatagccc agaaggtgca gaacgccatg cacggtcagg
     1621 tgcagagcat gtgcacaaag gccgatacca gcccttgttg cctcgcgctc cgcgacgccc
     1681 agcgcgagcg ccacgtcgcc aagataggct ttggccgccg ccgccggaaa tgcaaggacg
     1741 tttgtgggag catcaatgcc gcgaaattgc gcattcagtg cccgcagtcg ctcgtcatga
     1801 gtcagaagca aggtgagttg ggcggcgttc tggttgtgcg cgccacgcac cagtgcgagc
     1861 cgggcggcgt tgcgcaaaag cggaagcccc acaccgcgcc gccgccaacc tggctcctcg
     1921 acgacgacag aaatgctgtg cgtcattctt ccgtggcgcg gctgcggcgc acgtcccgcg
     1981 cggcagcccc cttgtcctga taggcgcgca cgatccgcgt gacgagggga tgccgcacga
     2041 cgtccgtgtc gctgaaatgc gccgtggcga cgccttcgac atgcgccagc gtggcaagcg
     2101 cctcgttgag gccttcgggc cgtccgccgg gaagatcgct ctgcgagggg tcgccggtca
     2161 ccaccatccg cgcatcttcg ccaaggcggg tcagaaacat cttcatctgt gcagacgtgc
     2221 agttctgcgc ttcatcgagg atcacgaagg cgcgcgcgag tgtcctgccg cgcatgaagg
     2281 cgaggggcgc cacctcgatc acgcccgact ccatcaggcg aacgcaaagc tctcccatcg
     2341 tctcgaacag cgcgtcgtag agtggccgca gatagggatc gatcttttcc ttgagatcgc
     2401 ctggcagaaa acccagccgc tcgcccgcct ccaatgcggg tctcgagagg atgagacgct
     2461 cgacgcggcg ctcatgcagc agatgtgcgc catacgctgc cgcaaggtag gtcttgcctg
     2521 tgccggcagg cccgatgctg aataccagag gatgcgtgcg catgagatcg aggtaggcgc
     2581 gttgcgccgg agagcgtgcg cgagcgactc gcgacgcagc cgtcttgatc gaacccgttc
     2641 cgaattccgc ggcgccgggc gtttccggtt catgcatcgc aaaacgaatt tccgcatcga
     2701 catcggccag cgcgacctcc tctccgcctt caagccgcgc gtagagcgcg cgcaggacgc
     2761 ctgccgcgcg ctcgcgcgac tccggagcgc cttcgaccga gatcaggttg ccgcgcgtcg
     2821 ccacgcgcac gccgagcttc tgctccacgc gcaccagatg tttctggtgc gcgccaccca
     2881 gcgcggcaag cttctgattg tcgggaaatt cgagcgtcac ctgctccctt ggaatgggtg
     2941 cgcggcgtgc ggctggcggt ctcaacagct gcccgccacg gcgttcgcgt tctgcgccac
     3001 aagttcgccc ttgaggctgt tggacagcac ctgtgcaatg cgcacatcac gaatgcggcc
     3061 gatgaggttg cgcgcattct cgacatggac gggttgcagc catggactgc ggccgatcgc
     3121 ctgtccgggc ttcgtgcccg gtttctcgaa gaggatcgac atggtgcttc ctgcgcaaag
     3181 ctggttgaac tccgcctgct gtgcggcgag caacgcttga agctgcgcga ggcgcgtcga
     3241 tttgatgtct tcaggtatct gcttcggcaa ggcggcagcc ggtgtgccgg ggcgcgcgct
     3301 gtatttgaag gagaacgcct gggcgtagcg cacgtcgcgg acgagctgca gcgtggcgtc
     3361 gaagtccgcg tcggtctctc cgggaaagcc cacgatgaag tcggaagaca aggcgatatc
     3421 ggatcgcgcc gcgcgaacgc gtgcgacgat ctacagatag cgttccgcgg tgtgttggcg
     3481 attcattgct gcgagaacac ggtccgatcc cgattgaacc ggcaggtgaa gatacggcat
     3541 caatttgacg acttcgcgat gcgcggcgat gaggtcatcg ctcatgtcgc gcggatggct
     3601 tgtcgtgtag cgcaaccgct cgatgccttc gatttcggcg agcgaagcaa tcagccgtgc
     3661 aaggctccag gcctttccat cggcagcaat gccgcgatag gcattgacgt tctggcccag
     3721 cagcgtgact tccttcacgc ccttttctgc aagctggcgc gcttcgtccg cgatccgctc
     3781 cggcggacgg gagaattcgg cgccgcgcgt atagggcacg acgcaaaacg tgcagaacct
     3841 gtcgcagcct tcctgtacgg tcagaaaagc ggatggtccg caatcgctgc gttcttcggg
     3901 cagcgaatcg aatttctcct gggcgggaaa ctccgtttcc aggacgtggc ccgcgccgcg
     3961 cgagatacgc gcgatcattt ccggcagccg atggtaggat tgcggtccca ccaccatgtc
     4021 gacggctttc tggcgcgcga tgatttcctc gccctccgcc tgcgcgacgc agcccgcgac
     4081 ggcgatcagc atcgggcggc cggcccgtgc cctgctgtcc ttcagcttct tcaggcggcc
     4141 gagctcggaa tacattttct cggcggcttt ctcgcggatg tggcacgtgt tcagaattac
     4201 cagatcggcg tcctcggggc ggtcgtcggg ctcatagccg attgccgcca gcaaatccgc
     4261 catgcgtgcg gaatcgtaga cgttcatctg gcaaccgaaa gtcttgacga acagcttctt
     4321 catctctgcg ccagttcgag gctcgcgggt tatgagtgaa gggtccaagc ttgacaagga
     4381 gttacgtcac gccgcggcag cggcacggga ttcctgccgt ggagcggtag tgcgaagctg
     4441 cgcacgggca tccggcggca agcctgcgag ggctcgtgcc tgtccccggc gcaccgccgc
     4501 ttccgccacg acggccagag ctttgcggct tccctcgatg tcgacacttg tagcttcatg
     4561 gaactcgacg accacgtcga gagggccagt ctgcatggct tcccataaat gcggcaccag
     4621 ctccatgtcg ccgtaccagg cgaacagcgg acgcatgtcg cgcgccatcg gcaaaccata
     4681 gaggccgaca taggatacag acacaggctg caccggaaca tgccgcgccc ttccctgcgc
     4741 atccgttccc agttccgctt cggctgcacc catcagcgcg ctcttgaagg aaagcacccg
     4801 gttgccgtca ttggacgttc cttccggaaa caggacgagc gcgtcgcctt gcgcgagccg
     4861 ttcgcgaatc aagtcgcggg cttcgacggt ctgcgaacgt tttgcgcgtt cgatgaacac
     4921 cgtttcctgg agcctcgcca acgttccaaa caagggccag ttcgcgacct cgcttttggc
     4981 gacgaaggaa acgcgcgccg cggacgagag aaccaggata tcgaaatagg aggtgtgatt
     5041 ggcgaccatc aacaccccgc tcgaacaaac gggacgtccg acgacggtta cgcgaatgcc
     5101 gaacaatcgg cagaggaaac gatgatagcg ctgcggaaac gtctttcgcc gtttgagccg
     5161 aaggcgcacc gccatctcct gccacgggat caggacgatg gtgacgagca gaaatacgga
     5221 gaggatggcg gcggcgcgca gggattgcac gtcaatgtcc gcgcttcctg gcgcccttcg
     5281 gcacgccgta gagttccagc cggtggtcga tcagctcgaa gcccagctct tcggcaacgc
     5341 gccgctgaag tttctcgatt tcttcgttgc ggaattcgat gacccgtccg gattgcacat
     5401 cgatcagatg gtcgtgatgc gagtctggaa cctcctcgta gcgcgaacgc ccgtcgcgga
     5461 aatcgtgccg ctcgagaatg cccgcatcct cgaacaattt caccgtgcgg tagacggtag
     5521 cgatcgagat atgtggatcg atcgccgccg cccgccggta caattcctcg gcatcgggat
     5581 gatcctccgc attcgacagc acgcgcgcga tcacgcggcg ctgatcggtc atccgcaggc
     5641 ccttttcggc gcaaagcttt tcgatccggc tcagtcggcc gtcccttccg cataggcgag
     5701 gcaagtgtag agcgtattgc gatcaaatag aaacaaagca tctcttgccc gggcaagcgc
     5761 caaggcacct atacattttc caaagtccgt catggccggg cttgttccca taggcgctaa
     5821 cttaagcagc tttccgcacg ccaaagtgta cccaccccca tagcgtgtca ttcccgcgaa
     5881 agcgggaacc cagtacgcaa agcgccagcg ctgttccctc tggatccccg ctttcgcggg
     5941 gaggacacgc ttttttggcg aagcgaagta atcttggcgc gtatggggct tgtcccggcc
     6001 gcccatgtgc acgctttgtg cccagaaagc gcgtttttgc cgagccgcac cggccaacgc
     6061 tggtgttcat gggtcgccgg gacaagcccc ggcgatgacg aaatttgtgg aaattcgtga
     6121 tgtgcagacg ccttagagcg caagtggggc atcgggggtg aacactcatt cagcgcgcca
     6181 actcatgatc gctgggttct cgccggagtt tatgagcgcc cgtttgagaa tgaggccgtc
     6241 gccgcggacg ccgcctgtgc cgtgataata agccggccgc gcgccaacct cgcgaaagcc
     6301 cagagcgccg taaagagctc tggcggcatc gttttcgacc gcgacttcga gacagatttg
     6361 cgcggcgccc atcgccgccg cccttgcggt cgccatgcgg atcagtcccg aaccgaggcc
     6421 ctggcggcgc gactcgtggc gaacgccgag cgacaggatt tcggcctcgc ccacgacggc
     6481 gcgtgcaacg acgaaccccg ccggctctcc ccgctccaac gccaccaggc tgaaagcgcc
     6541 gggctgggcg agaaggcggc ccaggaattc cggtccccag acct
//
LOCUS       DASZMC010000405         4751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4844200,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000405 DASZMC010000000
VERSION     DASZMC010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4844200"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VG274_08360"
     CDS             complement(<1..550)
                     /locus_tag="VG274_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWE06706.1"
                     /translation="MTFFTPREIVSELDRYIVGQSAAKRAVAIALRNRWRRQQLAPEL
                     REEVQPKNILMIGPTGVGKTEISRRLARLAQAPFLKVEATKFTEVGYVGRDVEQIVRD
                     LVEISLSQVRERRRREVETQAEAHAEDRILDALAGANAGQATREVMRKRLRAGELNDK
                     DIELEMRDAGGAPMIEIPGMPGA"
     gene            complement(547..1098)
                     /gene="hslV"
                     /locus_tag="VG274_08365"
     CDS             complement(547..1098)
                     /gene="hslV"
                     /locus_tag="VG274_08365"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HWE06707.1"
                     /translation="MDGKKDRWRSTTILSVRKAGSVVIAGDGQVSLGQTILKSNARKV
                     RRLGKGDVIAGFAGATADAFALFERLETKLEQHPGNLARACVELGKDWRTDKYLRRLE
                     AMMVVADKNAMLLLSGTGDVVEPEDGVLGIGSGGNYALAAARALMSFDIPAEEIARRA
                     LAIAARICVYTNDRIVTESIEIA"
     gene            1337..2449
                     /locus_tag="VG274_08370"
     CDS             1337..2449
                     /locus_tag="VG274_08370"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HWE06708.1"
                     /translation="MVQITKDGAYDAVAPDDFPAMMEPERYGRRSSAFDRIISATHDH
                     FWDPLNPKYIDFTEPFDLERQQILPDEMFPIFQTSLGERLKGSDHIRFANQSARWSLS
                     SILHGEQGALALSASLCHILSDPGAQEYAANQTREEARHVTAFSAYIRARWGAPLPCG
                     PTLQNLLTELVLAPEVYKKIVGMQMLVEGLAMGAFATLYQRSSDPLLVKLCQLVMTDE
                     AFHHKFGKIWADRTIPRLTPEEHNLVEDWAAQCFQTLLFNLVNPEQQKPVYASVGLDW
                     QEAMAAIQEAFGDDQRREQMKQSANIFRVLIKTLLNAGIVTGRSRAFYAVYVDMDELK
                     REGDRMVGDDIAEEGIAFLKTVNFRGSARTPLLAAE"
     gene            2511..2963
                     /locus_tag="VG274_08375"
     CDS             2511..2963
                     /locus_tag="VG274_08375"
                     /inference="COORDINATES: protein motif:HMM:NF018070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HWE06709.1"
                     /translation="MTTAAEVARGVSRLLIQEGFSPILEFSLANGRRLDVAALGADGT
                     VAGVEIKVAIPDLKGDAKWPDYLEFCELFYFAIPPEFPDELVPHQTGLIVADRYGGAI
                     VRPAPAGMLHPGRRKAVTLRFARVAAERLALTLELATEPRRVLGAIPE"
     gene            2960..3346
                     /locus_tag="VG274_08380"
     CDS             2960..3346
                     /locus_tag="VG274_08380"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HWE06710.1"
                     /translation="MTRRPSKAAQAAGLTSAQLRTIALSFPAASEGTSYGKPSFLVAG
                     KFFTRLRDEDDSLVLIVGSIDERDMLLESDPKLFHITNHYRNYPAVLARRTLFDPARL
                     RAMLERRWRAIAPKKLVKQIGAQQKP"
     gene            complement(3554..3892)
                     /locus_tag="VG274_08385"
     CDS             complement(3554..3892)
                     /locus_tag="VG274_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06711.1"
                     /translation="MRLLLDSNVLIRLADERLDTLAAPLRTALTAPDSEIHASVASLW
                     EIAIKHRLGKLALKVALPKLPELIANAGTPCLRSRRHMRLLRSIRNRRRAIRSTGCYW
                     LSARLKDSCC"
     gene            complement(3896..4180)
                     /locus_tag="VG274_08390"
     CDS             complement(3896..4180)
                     /locus_tag="VG274_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWE06712.1"
                     /translation="MAPRYAFGMTIVSITDAERRLAELAQIVERGETVVVTQDGKPVF
                     DLVPHPQNSGLRLGAIAEFKRKYGIDEIFPYVADDFDAPMPEDILLKPLR"
     gene            4541..4717
                     /locus_tag="VG274_08395"
     CDS             4541..4717
                     /locus_tag="VG274_08395"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWE06713.1"
                     /translation="MNTPHARITEELILRKLGAGRSFADILEAYPQLAEDDLRAAMAF
                     AADCLEHESVLAAG"
BASE COUNT          863 a         1469 c         1482 g          937 t
ORIGIN      
        1 gggcaccggg catgccgggg atctcgatca tcggggcgcc gccggcgtcg cgcatctcga
       61 gttcgatgtc cttgtcgttc agctcgccgg cgcgcaagcg ctttcgcatg acttcgcggg
      121 tagcctggcc tgcgttggcg cccgccagtg catcgagaat ccgatcctct gcgtgcgcct
      181 ccgcttgcgt ttccacttcc cgacgccgcc tttcccgaac ctgggagagg ctgatttcga
      241 cgagatcgcg cacgatttgc tccacgtcgc ggccgacata gccgacttcg gtgaacttcg
      301 tggcctcgac cttgaggaaa ggcgcctggg cgagcctggc gagtcgccgc gaaatttcgg
      361 tcttgccgac gccggtcggt ccgatcatca ggatgttttt cggctgcact tcctctcgca
      421 attccggcgc gagttgctgt ctgcgccagc gattgcgcag cgcaattgcg accgcacgct
      481 tggcggcgga ttggccgacg atgtagcggt cgagttcgga gacgatctcg cggggcgtaa
      541 aaaaggtcat gcgatttcga tgctttcggt cacgatccgg tcgttggtgt acacgcagat
      601 tcgcgcggcg attgcgaggg cgcgacgcgc gatttcctcg gcgggaatgt cgaaactcat
      661 gagggcgcgc gcggcggcaa gcgcatagtt tccgccggac ccgattccca gcactccatc
      721 ctccggttcc accacgtcgc ccgtgccgga gagcagcagc atcgcattct tgtcggctac
      781 aaccatcatg gcctccagcc ggcgcagata tttgtccgtg cgccaatcct tgccgagttc
      841 gacgcaggcg cgggcgagat tgcccggatg ctgctcaagc ttggtttcga ggcgctcgaa
      901 gagggcaaat gcgtccgcgg tcgcgccggc aaagcctgca atcacgtctc ctttgccaag
      961 acggcgcacc ttgcgggcat tcgacttcag gattgtctgg ccaagcgaca cctgcccgtc
     1021 gcccgcaatg acgacagatc ccgctttgcg gaccgagagt attgtcgtgg accgccagcg
     1081 atcctttttc ccgtccatgg tgtcaggccc ttgatgccgg cctcaaatag ggcggggcat
     1141 ctcgcgccgc aactggtgaa gcggcgcaag cgaagacgga gcgatcgctg tcggtcaggc
     1201 ggaggaaacg gcgttcgaag gcccgaaaaa ccatcgcgtg acactccgtc attaatcgtg
     1261 acgcatgcgt catttttgtt gacatcggac cattcggtgg ggatagtcgc actgctggtc
     1321 accgagggag cggtccatgg tccagatcac caaagacggc gcctacgacg cggtcgcgcc
     1381 ggacgatttc ccggccatga tggagcctga gcgctacgga cggcggtcga gcgcattcga
     1441 ccgcatcatc tccgcgaccc acgaccattt ctgggatccg ctcaacccga aatacatcga
     1501 tttcaccgag cccttcgatc tcgaacggca gcagatcctg ccggacgaga tgtttcccat
     1561 cttccagacg agtctggggg agcggttgaa gggcagcgat cacatccggt ttgcgaacca
     1621 gtcggcgcgc tggtcgctgt cgtcgatcct tcatggggaa cagggcgcgc tggcgctttc
     1681 ggcatcgctc tgtcacatcc tcagcgatcc cggtgcgcag gaatacgccg ccaaccagac
     1741 gcgggaggaa gcccgtcacg tgacggcctt cagcgcctat atcagggcgc gctggggagc
     1801 gccgctgccc tgcggcccga ccctccagaa cctgctgacg gagctggtcc tcgcgcccga
     1861 ggtctacaag aagatcgtag gcatgcagat gctggtcgaa gggctggcga tgggcgcctt
     1921 tgcgacactc tatcaacgat ccagcgatcc gttgctggtc aaactctgcc agctcgtcat
     1981 gacagacgaa gcgtttcacc acaaattcgg aaagatctgg gccgaccgca cgatcccgag
     2041 gctcactccg gaagaacaca atctggtcga agactgggcg gcgcaatgtt tccagaccct
     2101 gcttttcaac ctcgtcaatc cggagcagca gaaaccggtc tacgcatccg tcggcctcga
     2161 ctggcaggag gcgatggcgg caatccagga ggctttcggc gacgaccagc gccgcgagca
     2221 gatgaaacaa tccgcgaata tcttccgtgt actgatcaaa acgctcctca acgccggcat
     2281 cgtcaccggg cgcagccgcg ctttctatgc cgtctatgtc gacatggacg agttgaagcg
     2341 cgagggcgac cgcatggtcg gcgacgatat cgccgaggaa gggatcgcgt tcctcaagac
     2401 ggtcaatttt cgcggcagcg ccaggacgcc gttgctggca gcggaatgat cgaagcggcc
     2461 tcgtgccgcg gaactggatt ccgttcctgc tttgtgctta gaatagccac atgaccacgg
     2521 cggccgaagt cgcgcgcggc gtcagccgcc tgctgatcca ggaggggttc agcccgatcc
     2581 tcgaattctc gctggcgaac ggacgcaggc tcgatgtggc ggcgctgggt gcggacggaa
     2641 ccgtcgcggg cgtcgagatc aaagtggcaa ttcccgatct gaaaggcgac gcgaagtggc
     2701 ccgactatct ggaattctgc gaattgttct atttcgccat tcctcccgag tttcccgatg
     2761 aactggtgcc gcaccaaacg ggcctgatcg tggccgaccg ctatggcggc gcgatcgtgc
     2821 ggcccgcgcc ggctggcatg ctgcatcccg gcaggcgcaa agccgtgacg ttgcggttcg
     2881 cacgcgttgc cgcggaacgc ctcgcactga cgctcgagct tgcgacggag ccgcgcagag
     2941 tgcttggagc aatccccgaa tgaccagacg cccgagcaag gccgcgcagg ccgccgggct
     3001 gacctctgcg caactgcgca cgatcgcgct gtcgtttccg gcggcgagtg agggtacgtc
     3061 gtacggaaag ccttcgtttc ttgtcgccgg caagtttttt acgcgtcttc gggacgagga
     3121 tgactctctt gtcctgatcg tcggctccat cgacgagcgc gacatgctgc tggagagcga
     3181 tccgaagctc tttcatatca cgaaccacta ccgcaactat cccgccgtgc tggcgcgcag
     3241 gacgctgttc gacccggcgc gcttgcgcgc gatgctggaa cgccgctggc gcgcgatcgc
     3301 gccaaagaag ctggtcaaac agatcggcgc gcagcaaaaa ccatagtcgc gatctgttcg
     3361 ccggctgccc gttggtctcc gcgttcacct gctcgatgcg cccggtcgcg ggccaatagg
     3421 agcggttcgt caccgcgccg ttgcccgccg tctccggcgt cagatgctgc caggcatcta
     3481 tcgtattggc ggtcccgcaa tttctatgcg ggtttccagg ccagcacatg cggttcgagt
     3541 gcgccgtcga tcgtcagcaa caggagtcct tcaatcgcgc actgagccaa tagcaaccgg
     3601 tcgaacggat cgcgcgtcgg cggttccgga tcgagcgtag caagcgcatg tggcgcctcg
     3661 atcgcaagca gggtgtgccc gcgtttgcga tcagttcggg gagcttgggc agcgccacct
     3721 ttagcgccag tttaccaagg cggtgcttga ttgcgatttc ccagagactg gcaacgcttg
     3781 cgtgaatttc ggaatccggg gcagtcagcg cggtcctgag aggcgcggca agcgtgtcaa
     3841 gacgttcgtc ggcgagccga atgagaacat tgctgtcgag cagcaaccgc attggctaac
     3901 gtagcggctt cagtagaatg tcttcgggca ttggcgcgtc gaaatcgtcc gccacgtaag
     3961 ggaaaatctc gtctatgccg tatttacgct tgaattcggc aatggcccca agccgcagcc
     4021 cgctgttctg gggatgcggt acgagatcga acaccggttt gccgtcctgg gtgacgacaa
     4081 ccgtttcgcc cctttccacg atttgggcga gttccgctag ccggcgttcg gcatccgtga
     4141 tcgacacgat cgtcatgccg aaagcatagc ggggcgccat tcgccgggca acgggtcagg
     4201 tcggttcgtt ggtctccgtc ctgtttcgtt gcaaacaaag ctcgcgcatc gcgccgtttc
     4261 cggaggacgc acggggcgaa gccgttcatt acgtttctgc aaatcgcacg atcaccgttg
     4321 tccatttctc gatcggctgg ccgttgaccg aaccgggaaa aaatcgccag ttcgccgtga
     4381 cgtagttcgc cgcgtaccgg gaccagcgcg tcgtcgccgc tcgttaaaat gattgggatc
     4441 gagccgcggc gcgggcctcg gcgtcagtcg ccgaggattt gccgcatatg cgttccgggc
     4501 tatgatatgg ccgccattcg cgaaatcggt caaaaacgaa atgaatacgc cgcacgcccg
     4561 catcacggaa gaactgattc tgcgcaagct tggcgccggg cgttcgttcg ccgatattct
     4621 tgaagcctat cctcaactgg ccgaagacga tttgcgtgcg gcgatggcct ttgccgccga
     4681 ctgtttggag catgagagcg tcctcgcggc cgggtgatgc gctttctggc cgacgaaaat
     4741 gtatcgcgtt t
//
LOCUS       DASZMC010000406         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4018883,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000406 DASZMC010000000
VERSION     DASZMC010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4018883"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            697..1200
                     /locus_tag="VG274_08400"
     CDS             697..1200
                     /locus_tag="VG274_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06714.1"
                     /translation="MILGISIGAFTLLHVAITLIAVGSGLIVAGGMFASRRLPLTNAL
                     FLFTTALTSVTGFLFAIHGFTPAIGVGIVSCVILAIALYAYYGKRLAGAWRWIYVVTA
                     IASLYLNAFVLVAQSFIMVAALNALAPTQSEPPFAITQAVVLAIFVLIGIIAAINFRP
                     AAPAGFK"
     gene            1261..>1628
                     /locus_tag="VG274_08405"
     CDS             1261..>1628
                     /locus_tag="VG274_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE06715.1"
                     /translation="MTEPNMDLAHLGHVEIRTPKPDESLKFFTNVFGLTESGRNGDSV
                     YLRGWDDYERATLKLTASKTTGVGHTAFRTRSKEALERRVAVLKDTPHHVGWIDGDIG
                     HGPAYQCRDPDGHVIELYYET"
BASE COUNT          270 a          519 c          535 g          304 t
ORIGIN      
        1 cacgtaggct gccatcgggc cggccacttc gggccggacc cgttggaccg gtctgtgccc
       61 cctcctccgg caacggaggt ggttacgaca aacaccgggg gcgctgcact ggaggcggca
      121 ggggatgggt aacgcccatc cctccgtggc tccgtcccag gaagcgcgtg agctggagct
      181 gagcggtggc gttcggtcgc tgccgcgagt gccgcggtgg agcgccggta ggcgtgctgc
      241 cccctcccat cccctccccc aagcggggga gggtcagggt gggggcagcg ccgcaccgca
      301 aggtgcggag gctggtcctg cgcttaccgg cgttccgctt ccttttcttc ttaccgagag
      361 cgtgaggaag ggaaggaaga aaaaggaaaa attgctctgg gcagaccgga gatgctgttt
      421 gagattgttg atcgtgccag ccgaggcgga attgcgccag cgggacggag tggttttgta
      481 ccgcgcgccc tggcaggggc ggccctcgcg gctgctctgc aaaattgggc gcccacaacc
      541 ggcgccgctg caattcggcg agacgctcgc tactcgttct cgctgccgcg cggtagaaga
      601 gtccgcaaag gcgcaacgca cgtccgctat ggccggctgc accacgacgc agtaacatcg
      661 ccctcgatac tgcggcacga ggttgagggg gacgtcatga ttctcgggat ctcgatcggt
      721 gcttttactc ttttgcatgt tgccatcacg ctcatcgccg tcggcagcgg gctgatcgtc
      781 gccggcggga tgttcgcgtc gcggcgactg ccgctcacca acgcgctgtt tctcttcacc
      841 accgcgctga cgagcgtgac cggatttctg tttgccattc acggcttcac cccggcgatc
      901 ggcgtcggca tcgtgtcctg cgtgatcctc gcgatcgcgc tgtacgccta ttatggcaaa
      961 cgcctggccg gcgcgtggcg ctggatttat gtcgtcaccg cgatcgcatc gctttatttg
     1021 aatgctttcg tgctggtcgc gcagagcttc atcatggtcg cggcgctcaa tgcgctggcg
     1081 ccgacgcaat cggagccgcc attcgcgatc acgcaggcgg tggtgttggc gatctttgtc
     1141 ctgattggga tcatcgccgc aataaatttc cggccggcag cgcccgccgg attcaagtga
     1201 ccaccgcgga agggcgcgcg gccacaaacg cccgtaacct tccgtccgca ggagtgacgt
     1261 gtgaccgaac cgaacatgga tctggcgcat ctcggccacg tcgagattcg cacgcccaag
     1321 cccgatgaat cgctgaagtt cttcaccaat gtcttcggcc tgacggagtc cggccgcaac
     1381 ggcgacagcg tctatctgcg cggctgggac gattacgagc gcgcgacgct gaaactcacc
     1441 gcgtcgaaga cgaccggcgt cgggcacacc gcgttccgca cgcgcagcaa ggaggcgctg
     1501 gagcggcggg tcgctgtgct caaggacacg ccgcaccatg tcggctggat cgacggcgac
     1561 atcggccacg gcccggccta tcagtgccgc gatcccgacg gccatgtgat cgaactttat
     1621 tacgagac
//
LOCUS       DASZMC010000407         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1565081,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000407 DASZMC010000000
VERSION     DASZMC010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565081"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..>2384
                     /locus_tag="VG274_08410"
     CDS             425..>2384
                     /locus_tag="VG274_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWE06716.1"
                     /translation="MIRRKVVRGAVLLALIPIFICLTFLTAVAWWPYPAGVDAYPASS
                     SFVEDRNAVPLAALVAHDGQWRLPLRDEQINPHLLDAIVAVEDSRFYAHAGVDWRSAA
                     MAAWEDVRGLRIRRGASTLTMQVQRLRDPKPRTFFNKFEQAVRAAQVEKAAGKRQILV
                     EYVNRAPFGGNLVGAGAASWRYFGRPCSQLSLGEAALLAGLPQSPNRLRPDRYPQRAM
                     YRRNHVLDRMFALGMIDQKQHDEACREAVAATWRALPQDRDNPAPTAEGAMPTLAWLA
                     ARYAGATQHTTIDSGVQRQAAQAAGEHLARLESSGVTAAAVVVLDTQSAECLAAVSLS
                     RGTENVDLTRRPRSTGSTLKPFIYASAFESGLFTPETMLQDSPTAWAGYEPADYDHKF
                     RGTLSAAEALAESRNIPAMLVLAKVGVEPAVGLMEAAGLKTLARSPGRYGLSLAIGGA
                     EASAMELAQAYAMLGRGGVGREVRFAISREPGMTLVSNVGRALPAIDSLGGQCPPYVK
                     SASVGSALADALRGAHAPQERVRQGGPYEALHAPRCLTSSACWQTLTALSGADRTAAI
                     CPAAARLHVAWKTGTSNGHRDAWCAAVTRHRTVIVWLGNADGRASSALVGAEAAAPLA
                     LRLIASLESAPSEPWPTVIAKPEQKQPDR"
BASE COUNT          405 a          757 c          783 g          439 t
ORIGIN      
        1 atggcccctc accccaaccc tggcctgatg aaaatgagtg tgttcctttc gtctgacatt
       61 tgttgcgcgt cattctgagg aggctttgcc gacgaagaat cttgcggcga ggtttgcggc
      121 acaactcctc ggcaagatgc ttcgtcggca agcctcctca gcatgacgcg caacagaact
      181 tgtatggaga acaaattcaa aatcccgccg aaaaggtaag ttattcatca gcccagggtc
      241 ggggtgagga gccgggcggt ggagtgcgct gctatccgaa tctcccctca ccctaaccct
      301 ctcccccgag tacgggggag aggggaccag agcagattgt gcaagtttcc gcaatatgag
      361 caagatcatt tcagcctcgt ccgcaggctt cccaacgaac cgcagccacg tcgcaatttc
      421 gcgaatgatt cggcgaaagg tcgtgcgcgg cgccgtcctt cttgccctca ttcccatttt
      481 catctgcctt acatttctca cggcggtagc atggtggccc tatcccgccg gtgtggatgc
      541 atatccggct tcttcctcat tcgtcgaaga tcgcaacgcg gtcccgctcg cggcgttggt
      601 ggctcacgat gggcaatggc gtcttccgtt gcgggatgag cagatcaatc cgcatctgct
      661 cgatgcgatc gtcgcggtcg aggacagccg gttctacgcc catgccggcg tcgactggcg
      721 ttcggccgcg atggcggcgt gggaggatgt gcgcgggctg cgaattcgcc gaggggcgag
      781 cacgctcacg atgcaggtgc agcggttgcg cgatccgaag ccaaggacat tcttcaataa
      841 gtttgagcag gcggtccgcg cggctcaggt ggagaaggcc gcggggaaac ggcagattct
      901 cgtcgaatac gtcaaccgcg cgccattcgg cggaaatctt gtcggcgcgg gcgccgcaag
      961 ctggagatac ttcgggcggc cctgctcgca actcagcctg ggtgaagcgg cactcctggc
     1021 gggattaccc cagtcgccga accggcttcg gccggatcgc tatccccagc gcgcgatgta
     1081 tcgccgaaat catgtgctcg atcgaatgtt tgccctcggc atgatcgatc agaagcagca
     1141 tgatgaagcc tgccgtgagg cagtcgctgc gacgtggcgg gcgttgcccc aggatcgtga
     1201 taacccagcg cccacggcgg aaggcgccat gccgacgctg gcgtggcttg ccgcgcgata
     1261 tgccggcgcg acgcagcaca cgacgatcga ctcgggagtg caacgccagg ccgcacaggc
     1321 ggcgggcgag catctggccc ggctcgagag ctccggcgtg accgcggccg ccgttgtcgt
     1381 gcttgatacg caatcggccg agtgcctggc cgccgtcagt ctttcgcgtg ggactgagaa
     1441 tgtcgacctc acgcgccggc cgcgctcgac ggggtcgacg ctcaagccgt tcatctatgc
     1501 atcggcgttc gagtcgggac tcttcacgcc cgagaccatg ctgcaagatt ctccgaccgc
     1561 ctgggccggt tacgagccgg ccgactatga ccacaaattc cgcggcaccc tgtcggcggc
     1621 cgaggcattg gcggaaagcc gcaatatccc cgcgatgctc gtgctggcga aagtgggcgt
     1681 cgagcctgcg gtggggttga tggaagcggc gggattgaag accctggcgc gctcgccggg
     1741 gcgctatgga ttatcgctgg cgatcggagg cgccgaagcg tccgcgatgg agctggcgca
     1801 ggcgtacgcg atgctggggc gcggaggcgt tggccgggag gttcgttttg cgatttcgcg
     1861 cgagcccgga atgactctcg tctccaatgt agggcgggca ctgcccgcca tcgattcgct
     1921 cggcgggcag tgcccgcctt acgtgaagag tgcatccgta gggtccgcct tggcggacgc
     1981 gctgcgcggt gcgcacgcgc cgcaagaacg cgtccgccaa ggcggaccct acgaagcgct
     2041 tcacgcgccg cgatgtctta cctcctccgc ttgctggcag accttgaccg ccctcagtgg
     2101 cgccgatcgc acggcggcca tctgtcctgc cgcggcgcgc cttcacgtcg cgtggaagac
     2161 cggtacgagc aatggccatc gcgacgcctg gtgcgccgcg gtcacgcgtc atcgcactgt
     2221 tattgtctgg ctgggcaacg ccgacggacg agcgtcgtcc gcgctcgtcg gcgccgaagc
     2281 cgccgccccg ctggcgctgc ggctgatcgc gagccttgag tcggcccctt ccgagccgtg
     2341 gccgaccgtt atcgccaagc ctgaacagaa acagccggac cgtc
//
LOCUS       DASZMC010000408         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_163555,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000408 DASZMC010000000
VERSION     DASZMC010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163555"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VG274_08415"
     CDS             complement(<1..1330)
                     /locus_tag="VG274_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06717.1"
                     /translation="MYAARARRTAGLSLEASLGPAQAGAALRLIQTGFVRAANAARAA
                     IDAVAPFVDAAFAPGTPGFKLLGTPLRNALELERAAAGWFAFDPAPQEAAAGGYWLAD
                     IFLRGTEPAEVAPLTRFLIGGTTVPIAQGRMRSIRRYPTGGLSEKQALILPPLLRAIA
                     ADMGWCSPFLVAKRLAHTGGTLDKLGTLPGFAPTSAAALAEWTGRPLPVLYVAAGEDL
                     CPRDAAMYRMRGDTGTVVDHGLMAASILSKQAAAPVDGLVLDVLHGSTAFLRDLEDAI
                     AFGELCIKVGAELGISVTTSYRNASAPMGRSVGASTEVLEAVGLLDPSTTDPRSVEID
                     RALGFVGALAGAAGLDGRRAVVLAKGALRSGATCRHLFDLWLDHGVDPAFVTRVARDP
                     RSAILGQLGRVEVRAPLTGIVQCDWVALADVVNSRINGFQAAGDGPPKLNP"
     gene            complement(1363..1977)
                     /locus_tag="VG274_08420"
     CDS             complement(1363..1977)
                     /locus_tag="VG274_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWE06718.1"
                     /translation="MLIAFAGLPSAGKSTTAKALADRLGVRWFVEPEEDQWPEAVRDR
                     DRVGRFTALTWFRSVRVPKLYAAAQASRSEHAAVIDSYYDVLLSRYIGEDPFSWLLDP
                     GDPYFPLARHMAELDWQLLPHADFLVFLRLDERVWNTFMNRRARDFDRAAHLKEQFAM
                     QALMEHACRQAEVEHGTRLIVIDQEDSTPAATAVRVAAFLPSIV"
     gene            2355..>2732
                     /locus_tag="VG274_08425"
     CDS             2355..>2732
                     /locus_tag="VG274_08425"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWE06719.1"
                     /translation="MKAGIVIFPGINRERDMAIALERSGATPRMIWHKETDLAGLDLI
                     VIPGGFSFGDYLRCGAMAAHSPVMPAIRAHAERGGYVLAVCNGFQILTEAGLLPGALL
                     RNATLRFLARDCHLRVERTDTAFT"
BASE COUNT          484 a          963 c          846 g          439 t
ORIGIN      
        1 ccggattgag cttgggaggg ccgtccccgg ctgcttggaa accgtttatc cgactgttca
       61 cgacatcggc gagcgcgacc cagtcgcact gcacgatccc ggtcaggggt gcccggacct
      121 ccactctccc aagttgcccg aggatggcgg atcgaggatc acgcgcgacc cgcgtcacga
      181 aggcgggatc aaccccgtga tcgagccaca ggtcaaagag gtggcgacag gtggccccgg
      241 aacggagggc acccttggcc aggactaccg ctcgccggcc gtcgagccct gcggcgccgg
      301 ccaatgctcc aacgaagcct agcgcccgat cgatttcgac ggaccgggga tcggtagtcg
      361 aagggtccag tagcccgacg gcttcaagta cctccgtgct tgcgccgacg ctgcgtccca
      421 ttggggcgga tgcgttgcgg taggacgtag tgactgaaat gcccagttcc gctccgactt
      481 tgatgcagag ctctccgaag gcaatcgcat cctccagatc gcgcaagaat gccgttgacc
      541 catgcagcac atcgagtacg aggccgtcta ccggagccgc cgcctgtttg gacagtatgc
      601 tggccgccat cagcccgtgg tccacgaccg tccctgtgtc cccgcgcatc cggtacatcg
      661 cggcatcccg tgggcagagg tcctccccgg ctgcgacgta gagcaccggc agcggcctgc
      721 cagtccattc tgcgagtgcg gccgcggagg tcggggcgaa gccgggcagt gttccgagtt
      781 tgtccagtgt cccgccggtg tgcgcgaggc gcttggctac caggaaaggc gaacaccaac
      841 ccatgtcggc tgctatggcc cgcagcagcg gaggcaagat aagtgcctgt ttctctgaaa
      901 gaccgccagt cgggtatctc cttatcgatc gcatcctgcc ttgagcaatg ggcacggtcg
      961 ttccgccgat gaggaagcgg gtaagtgggg ccacctccgc tggttccgtc ccgcgcagga
     1021 agatatccgc aagccagtag ccgccggccg ccgcctcctg cggagccgga tcgaacgcga
     1081 accagccggc cgccgctcgt tcgagttcga gtgcgttgcg caagggggta ccgagtagct
     1141 tgaatcccgg cgtgccaggt gcgaatgcgg catcgacaaa gggagcgacc gcgtcgatcg
     1201 cggctcgcgc cgcattggcc gcccgtacga agcccgtctg gatcaggcgc agggcggcgc
     1261 cggcctgcgc cggtccgagg ctagcctcga gcgacagtcc ggctgtgcgc ctggcgcgcg
     1321 ctgcgtacat gacgcgcgcc agagttaaag ccgcgctcgc agtcacacta tgctcggcag
     1381 aaacgccgcg actcggactg ccgtagcggc gggcgttgag tcttcctggt cgatcacgat
     1441 gaggcgcgtt ccgtgttcga cctcggcctg ccggcacgcg tgctccatca gcgcctgcat
     1501 agcgaactgc tccttgagat gcgccgcccg gtcgaagtcc cgggcacggc gattcatgaa
     1561 cgtgttccag acccgctcgt ccagccgtag gaacacgaga aaatccgcgt gcggcagcag
     1621 ttgccagtcc agctccgcca tgtgccgcgc gagcggaaag tacggatcac ccggatccaa
     1681 cagccacgag aaaggatcct ccccaatgta gcgtgagagc agtacgtcgt agtagctgtc
     1741 aatcacggcg gcatgctccg atcggcttgc ttgcgcggcg gcgtagagct tggggacgcg
     1801 tactgatcgg aaccatgtga gcgcggtgaa gcggccgacg cggtcgcgat cacgcaccgc
     1861 ctccggccat tgatcctcct caggttccac aaaccacctt acgcccagcc tatccgcgag
     1921 cgctttggcg gtggtggatt tcccggcgct tggcaggccg gcaaacgcga tcagcaatca
     1981 tcacctccta tcaatacgat gcaggaacgc taactttccg gcaccgcgat cgcaagcgcg
     2041 acggagccat ggcgaagttc gagcttgatc ggatcgatgt cggcggagag gttccacctg
     2101 gaaggcgctg gcagtcaatg tgcgcttgaa cccagtttga ttcgctaacc acgtccgcat
     2161 tgcctatttc cagccgagaa gccgctatgc cgctcccgcc gaccaccggc gcatcgtgcc
     2221 aaatacctgg aacgtccgcc ccgccccgga accgcccccc cacccccgcc acacgactcg
     2281 cccgcccacg ctggcggacc acccgcaccc gtggcaagaa ccgccacgcc aacccaccca
     2341 acgccgaggc gcgcatgaaa gccggcatcg tcatcttccc cggcatcaac cgcgagcgcg
     2401 acatggcgat cgcgctggaa cgctccggcg ccaccccgcg catgatctgg cacaaggaga
     2461 ccgaccttgc cggcctcgac ctgatcgtca tccccggcgg cttcagcttc ggcgactacc
     2521 tccgctgcgg cgccatggcc gcgcactccc ccgtgatgcc cgccatccgc gcccacgccg
     2581 aacgaggcgg ctacgtcctg gccgtctgca acggcttcca gatcctgacc gaggccggac
     2641 tgctccccgg cgccctgctg cgcaacgcga cgctgcgctt tctcgcccgc gactgccacc
     2701 tgcgcgtcga gcgcaccgac accgccttca cc
//
LOCUS       DASZMC010000409         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1303842,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000409 DASZMC010000000
VERSION     DASZMC010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303842"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VG274_08430"
     CDS             <1..899
                     /locus_tag="VG274_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE06720.1"
                     /translation="NTRAVDGISFRVEEGETLGLVGESGCGKSTTALALMRLLRDASL
                     TGRAWLDGVDLIQLGEPEMRAMRGSQVGMIFQDPATALHPMVRVGEQVAEVLDAHLSL
                     SRAETRARTVELFRQVGIAAPERRIRAFPHELSGGMCQRVVIAMAIACGPRLLLADEP
                     TTALDVTVQAQILDLLRGIASQSRAGMVLITHDLGVVAGMADRVAVMYAGRIVESGPT
                     DQIFARPLHPYTAGLLASIPQLDSGWDEPVPTIPGSVQNRSAGSGCPFAPRCPLASME
                     CRTAPPFLPKDGGRMVACWKAV"
     gene            899..>1546
                     /locus_tag="VG274_08435"
     CDS             899..>1546
                     /locus_tag="VG274_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HWE06721.1"
                     /translation="MAEALVEAIGVSRLYRASRNRVVRAVDHVDMKIGRGTTLGLVGE
                     SGSGKSTLGRTLVGLQTPSLGSVAFEGQDVAGASGPRLIRLRQQRQIVFQDPSGALNP
                     RMTIGDTVVEHLRVQGWTRADARSRAQEVFEQAGLSRQFLPRYPHEISGGQRQRAVIA
                     RAISTDPAFIVADEPVSALDVSTRAQIMNLLRELQASRGMTMLFISHDLSVVAHMC"
BASE COUNT          257 a          483 c          528 g          278 t
ORIGIN      
        1 cgaacacccg ggccgtggac ggcatttcgt ttcgcgtcga ggaaggtgaa acgctcggcc
       61 tggtcggcga aagcggatgc ggcaagtcga cgaccgctct cgcgctgatg cgattgttgc
      121 gcgacgcttc gttgaccggt cgcgcctggc tcgacggcgt cgatctcatt cagctcggtg
      181 aacccgagat gcgtgcgatg cgcggaagcc aggttggaat gatttttcag gacccggcga
      241 cggcgctcca tcccatggtt cgcgtcggcg aacaggtcgc cgaggtgttg gatgcccatc
      301 tttccctttc cagagccgaa accagagcgc ggacggtcga gctgttccgc caagtgggaa
      361 ttgccgctcc cgaacggcga atccgcgctt ttccccacga gctttcaggc ggcatgtgcc
      421 agcgcgtggt gatcgccatg gcgatcgcct gcggtccgcg cctgttgctg gcggatgagc
      481 ccaccacggc gctcgacgtc acggtgcagg cgcaaattct ggatctgctc cgggggatcg
      541 cgagtcaaag cagggccgga atggtcctga ttacgcacga cctcggcgtc gtcgctggaa
      601 tggccgatcg cgtggcggtc atgtatgccg gaaggatcgt cgaatccggg cccacggacc
      661 aaatattcgc ccgcccgctt catccctaca ccgcgggtct gctggcctcg atcccgcaac
      721 tcgacagcgg ttgggacgag ccggtgccca ctatccccgg gagcgtgcaa aacaggagcg
      781 caggcagcgg atgtccgttc gcgccgcgat gcccgctggc ttcgatggaa tgccgcactg
      841 cgccgccgtt cctgccgaag gacggcgggc gaatggtcgc ctgctggaag gccgtgtgat
      901 ggcggaggcg ctcgtcgagg ccatcggcgt gagccgcctc taccgagcta gtaggaaccg
      961 cgtcgtgcgc gcggtcgacc atgtagatat gaagatcggc cgcggcacga cgctcggact
     1021 cgtgggagaa agcggcagcg gcaagagcac cttgggccgg acgctcgtcg ggctgcaaac
     1081 gccatcgctc ggctcggtcg cgttcgaagg acaggacgtc gcgggcgctt cgggcccgcg
     1141 gctgattcgc ttgcggcagc aaagacagat cgtgtttcaa gacccttcgg gggcgctgaa
     1201 tcctcggatg acgatcggcg acaccgtcgt cgaacatttg cgcgtgcagg gctggacgcg
     1261 cgcggacgcg cgctcgcgcg cgcaggaggt gtttgaacag gcgggccttt cgcgccagtt
     1321 tcttccgcga tatccccatg aaatttcggg cggacagcgc cagcgcgcag tgatcgcgcg
     1381 cgcgatcagc accgatccgg ccttcatcgt cgcggacgaa ccggtgtccg cgctggatgt
     1441 gtcgacccgt gcgcagatca tgaatttgct ccgcgagttg caggcgagcc gcggcatgac
     1501 catgctgttc atcagccacg atctcagcgt cgtggcgcat atgtgt
//
LOCUS       DASZMC010000410        12109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3200339,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000410 DASZMC010000000
VERSION     DASZMC010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3200339"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VG274_08440"
     CDS             <1..334
                     /locus_tag="VG274_08440"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWE06722.1"
                     /translation="VPELDRTLKSVFQADTYPLASFSATRVTRTGENTGVVAGNLTIA
                     NVTKPVNLNVTFNGGRGSGEPMQPYRVGFDATATLRRSDFGLTRMIWSGFVSDDVQLV
                     IEAEAVRR"
     gene            complement(420..2096)
                     /locus_tag="VG274_08445"
     CDS             complement(420..2096)
                     /locus_tag="VG274_08445"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HWE06723.1"
                     /translation="MEQTAPAAATPCRRSRRRRRDAWCTSLLAAIVAGAMPACAAELL
                     QSDGWDVRWDNTLRYTAAFRLAHYDDALVADPNADDGDRNFAPGLISNRLDLLSELDL
                     SNGPFGVEVSAAAWYDTVYHAANSNDSPATFNPESVPHNRFTHAVEILHGEDADLLNA
                     FAYGGFDLDDMPVSFRVGRHTLLWGESLFFADNGIAAGQAPIDAIKAASQPEAEAKEV
                     FLPVGQASATIQPRPWLAFSLYDQFEWRRTRLPGSGSYFSDADYLDAGGERLIVAPNQ
                     FLYRTADVVPPSSGQYGAAMHVTLGDVDYGLYALRFNAKEPEIFIFTSPQPMGGNAGD
                     YWLVFPTGIQTYGASFSTYVSDSTLAGEISVRRNMPLVSQTLFSLPGGNGGGYGGAVY
                     AALQAPGHGEAANNISTVTSYAVGDTLHAQLSSVSTLSPGALWDSADLSAELAANDRL
                     SVTSGLAELATARDRLAAAMRAVLEPHYFQVLPALDISVPIGLGFDLLGRSSVNSSMN
                     YGAGDLELGLTATWRTVWEGSVTFTHFFGPVDRQPFADRDFISISIQRTF"
     gene            2193..3560
                     /locus_tag="VG274_08450"
     CDS             2193..3560
                     /locus_tag="VG274_08450"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HWE06724.1"
                     /translation="MRCIVALALCVAVGGSMSPAPARAAVSARQAEQLKTVLTPLGAE
                     RAGNREQTIPAWTGGYTSVAASYREGGPRPDPFAGEKPLFSITANNLDAYAGRLPEGA
                     KALFGKYPDYRMDIYPTHRSAAAPASVYANVLLNATRAHAAPEGIAYGVAGAAGGVPF
                     PIPQNGFEAMWNHLLAFWGPARELQLKTWVVSADSTIELASGYREVADFPYYYASATP
                     DSYGGYYFKTLHLEDAPASKVGEGYLDWQPIDIARFNFDAWRVLPGEHRVRRGPAISY
                     DTPDSDAAGFESLDEYYIFFGGLDRFDFKLLGKKEMYIPYNNNRFYTRPTSEILGTRH
                     ANPDDLRYELHRVWVVEGTLKPGAHDVVSRRRLYLDEDTWLAVYSDSWNDRGKIWKFG
                     QGTMYLMPEIPAVILGSQFVYDLDLGGYVFGFAFNDQPEAYKVTAPHSASLFTPESMA
                     AHAVR"
     gene            complement(3883..4221)
                     /locus_tag="VG274_08455"
     CDS             complement(3883..4221)
                     /locus_tag="VG274_08455"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HWE06725.1"
                     /translation="MGLFDGLLGGIVGAEMATVVNGLIERHGGVQGVVNQLRSNGLGP
                     SVNSWINEGPNAPVAPQDVHRAFGDQTMSELAAKSGMSTEELAQKLAQVLPHAVDALT
                     PGGEVLRTKE"
     gene            complement(4300..4938)
                     /locus_tag="VG274_08460"
     CDS             complement(4300..4938)
                     /locus_tag="VG274_08460"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWE06726.1"
                     /translation="MSLRVIGAGCGRTGTASLKFALERLLGAPCYHMIEVFQHPGHSA
                     IWRQAALGEATDWDALFAGYAAAVDWPAAAFWPELMRAYPGALVLLSLRDAEDWWASA
                     SQTIFPSIQSHPHASPEWKAMIADMFRTRWGADLNDRNASIAAYNAHNARVMETVPKE
                     RLLVWRAGEGWEPICGLLGVPVPAEPFPRSNTREEFRARAKAYAEQAGEKVA"
     gene            complement(5006..6406)
                     /locus_tag="VG274_08465"
     CDS             complement(5006..6406)
                     /locus_tag="VG274_08465"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06727.1"
                     /translation="MIAIASALLSAVGFYFSFGLGNLWWLAWIAPVPILWFAFGDARP
                     WTVFIACFAACALGATSILRAYAGLLPVPVLIFYICAPALTFALAVLGARRVQPVLGA
                     VLAMFAFASLWTAFDFLASFDSGGGSVATPAAAEVGAPFLIQSASLVGYLGITFLLGF
                     ISAGIALSLRARDPVPAALALMLFVANAAYGIWRMSTPPTGSIRVALIDSNDVAGPTR
                     RSDTAAALKDIDAYAAQIAKLNGAQAKLIVLPENIAQIAPDWRNAAQAKLAATANDAG
                     ATVVAGFNTFLDGAQRNVSWAFVPGRLRPVTYMKRRLVPVLESAFYKPGPGPRVLSNG
                     TGLEICKDMDFQAMIRVDETATKPVVLAVPAWDFDRDDWSHARVAVLRSVENGVPMAR
                     TARDGLLTLNDRYGRIISAVRTRAGFRTLIGDLPVGERGGATFYDRIGDAFGWICIAL
                     GIGLVGWSSVKRKSPA"
     gene            complement(6811..7560)
                     /locus_tag="VG274_08470"
     CDS             complement(6811..7560)
                     /locus_tag="VG274_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06728.1"
                     /translation="MTTELRDEDLHAYIDGELDDFGRSRVQSIVDADPVLRHQLSLYR
                     ADKARLVSLYGAGLNEPLPREWIERIEGRGARRQWNSRTNWAIAAMAATVVLVLAGSI
                     AYRNFAPAARADIVADALAARTQSLPPDTVIPIRSTRDAEVEAAVMTRVLATQVKAPD
                     LTKLGFRLVGMDVYNTPTRSFDLRYRDGKGRVFTLYLRRSSGAPRFDLFEEKGLHVCV
                     WQDEVMATVMAGPVSGAEMERLAGLVYTSLT"
     gene            complement(7557..8105)
                     /locus_tag="VG274_08475"
     CDS             complement(7557..8105)
                     /locus_tag="VG274_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWE06729.1"
                     /translation="MVGSRRESFGKALITQLPALRRYARALVGNSALADDLVQDCIER
                     ALRQWEGLRDGQRLTSWLRSILHNLYVDELRRNRTRGHEQEIAELADELSFSAPAHDG
                     AMAFEFVRAMDTLSVEHRQVLLLVGLEDMNYREIATELGIPIGTVMSRLARGRERLRS
                     ALEGERRQSGAVHVPGAWEMRQ"
     gene            8315..8391
                     /locus_tag="VG274_08480"
     tRNA            8315..8391
                     /locus_tag="VG274_08480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8349..8351,aa:Arg,seq:cct)
     gene            complement(8746..9714)
                     /locus_tag="VG274_08485"
     CDS             complement(8746..9714)
                     /locus_tag="VG274_08485"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HWE06730.1"
                     /translation="MRILPGMATLATVLAVAGCSSDASRAGVSDDPYESANRSSFASH
                     QILYRNVIRPVVVSYNDAVPEPARDGVHNFLVNIDLPVTFGNDLLQGKIVRGGETLAR
                     FTINSTAGIGGIVDVARKIGIPEHETGFADTMADYGVGEGPYLYVPVIGPSVPRELAG
                     KLVDTAFDPLTYVTYGASIFVSVGRTGATFVDKRARGAATADKIEKISRDPYATTRTL
                     YQKHHAAEADDTATTPDFDAGPADVRLAAQQSRETVSSAAPSDSQDVGGHCSSVAELR
                     ASDAAASGYESGMQQIVRDSVYKDCMSWKVAHGVVGTISGQAVALQ"
     gene            complement(9768..9947)
                     /locus_tag="VG274_08490"
     CDS             complement(9768..9947)
                     /locus_tag="VG274_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06731.1"
                     /translation="MVFSFNPGSGLKPDIIRYCAAPLLCESLGVCDISAGFCFDELTN
                     ILLYKRTTLRFAAMA"
     gene            complement(9989..10426)
                     /locus_tag="VG274_08495"
     CDS             complement(9989..10426)
                     /locus_tag="VG274_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE06732.1"
                     /translation="MTRTENVQEIVFPDPEERTPAILVVEDEVLIRIALSDYLQECGF
                     KVLEAGNADEALEIIQHSPVVIDLVFSDVVMPGKLDGFGLAHWIRINQPGLPIILTSG
                     DAAKAATAKDLCEREPFMAKPYDLQHLVRRIRTLIDSARKKSP"
     gene            complement(10720..11109)
                     /locus_tag="VG274_08500"
     CDS             complement(10720..11109)
                     /locus_tag="VG274_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06733.1"
                     /translation="MFELFGHALIVADDLVECVGDLAEEAGFVTCHAHRKIARLHRLQ
                     RMQQFMQFCGVTVQPVARFTRTLHGHGRSGGAFSRLVRGCSFGLRRHWKKLPLTFVNW
                     RTRANGRELLARNRDRQQNCRFFKGIR"
     gene            11089..>12109
                     /locus_tag="VG274_08505"
     CDS             11089..>12109
                     /locus_tag="VG274_08505"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWE06734.1"
                     /translation="MAEQLEHVGRLVGREGQTKHRVKLGRLSGAWGAMESSINVLIDD
                     LLWPTTEVTRAISAVAQGNLLQTVRLDVEGRPLKGEFLQAATIVNTMIKQLGVFTSEV
                     TRVAREVGTEGKLGGQAQVREVTGVWKDLTESVNSMASNLTAQVRNIADVTIAVAMGD
                     LSKKITVDVRGEILQLKEAINTMVDQLRSFASEVTRVAREVGTDGKLGGQAIVPGVAG
                     TWKDLTDSVNAMCGNLTDQVRNIAQVTTAVARGDLSRKITVDVRGEILELKDTINTMV
                     DQLNAFASEVTRVAREVGTEGKLGGQAQVPGVAGTWKDLTDTVNVMAANLTEQVRGIV
                     KVVTAV"
BASE COUNT         2358 a         3851 c         3733 g         2167 t
ORIGIN      
        1 cgtacccgaa ctcgacagaa cgctgaagtc cgtctttcaa gccgatacgt atcccctcgc
       61 cagcttcagc gcgaccaggg tcacgcggac gggcgaaaat accggcgttg ttgcaggtaa
      121 tttgacgatt gcgaatgtca ccaagcccgt caatctgaac gtcaccttca acggcggccg
      181 cggatctggt gaaccgatgc agccgtaccg cgtcggcttc gatgccacgg ccactctcag
      241 gcgttccgac ttcgggctta cgcgaatgat atggtccgga ttcgtcagcg acgacgtcca
      301 gcttgtcatc gaggccgagg cggtcagacg ataaccggag gttttctccg gcggaacaca
      361 tggcggtcgc aaatggaggt tggcacgcca ccgcacgttt tttcgcggct cgcttccatt
      421 caaaacgttc gctggatgct gatcgagatg aaatcccggt cggcgaaggg ctgccggtcc
      481 accggaccga agaaatgcgt gaacgtgacg gatccttccc agaccgtgcg ccacgtcgcg
      541 gtcagcccga gctctaggtc gcctgcgcca tagttcatcg aggaattgac gctcgatctg
      601 ccgagtagat cgaaacccag accgatgggc acggagatgt cgagcgccgg cagcacctga
      661 aaataatgcg gttccagaac cgcccgcatg gcggcggcca ggcgatcgcg ggctgtggca
      721 agctccgcaa ggccgcttgt gacgctgagc cgatcattcg ccgccagctc ggcgctgaga
      781 tcggcgctgt cccacaacgc gccgggagaa agagtcgata cgctcgaaag ctgcgcatgc
      841 agcgtgtcgc cgaccgcata tgaggttaca gtggaaatgt tgttcgccgc ctcgccgtgt
      901 cccggcgcct gaagcgctgc gtaaaccgcg ccgccgtagc cgccgccgtt tccgcccggc
      961 agcgaaaaaa gcgtctggct gaccaggggc atgttgcgcc ggacagagat ctctcccgcc
     1021 agcgtgctgt cgctcacata ggtgctgaag ctcgcgccat aggtctgaat tccggtgggg
     1081 aacacaagcc aataatcgcc cgcatttccg cccattggct gcggcgacgt gaagatgaaa
     1141 atctccggct ccttcgcgtt gaatcgcagt gcatagagcc cgtaatcgac atcgccgagc
     1201 gtgacgtgca ttgccgcgcc gtattggcct gacgaaggcg gcacgacgtc cgcagtgcgg
     1261 tagagaaact ggttcggggc gacgatcagg cgttctcccc ccgcgtcgag ataatcggca
     1321 tcgctgaaat agctgcccga gcctggcaac cgcgttcgcc gccactcgaa ctggtcgtag
     1381 aggctgaaag cgagccaggg tcgcggctga atcgtggccg aggcctgccc caccggcagg
     1441 aacacttctt tcgcttccgc ttccggctgg ctcgccgcct tgatggcgtc gatgggcgcc
     1501 tggccggcgg caattccatt gtccgcaaag aacaggcttt cgccccacag gagcgtgtgc
     1561 cgtccgacgc ggaacgagac cggcatgtcg tcaagatcga agccgccata ggcaaacgcg
     1621 ttcagcagat cggcatcttc gccgtgcagg atttcgaccg cgtgcgtgaa gcggttgtgc
     1681 ggaacgcttt ccggattgaa cgtggccggc gagtcgttgc tgttcgcggc gtgatagacg
     1741 gtgtcgtacc aggccgcagc gctcacttcc acgccgaacg gaccattcga cagatccagt
     1801 tcggacagga ggtcgagccg gttggaaatc agtcccggcg cgaaattgcg gtctccgtcg
     1861 tccgcattgg gatcggcaac cagtgcgtcg tcataatggg ccagccggaa cgcggccgtg
     1921 tatctgagcg tgttgtccca gcgcacatcc catccatcgc tctgcagaag ctcggcggcg
     1981 cacgcaggca tcgcgcccgc gacaatggcg gcgagcaggg acgtgcacca cgcgtcccgg
     2041 cgcctgcgcc gcgatcgcct gcatggtgtc gccgccgcag gggccgtctg ttccaccctc
     2101 gtcccccgaa ttggtgtccg cgctggtgcg atgatgtata agtgaacata caggacttgc
     2161 gggtttcccc aaaacaccgg aaagggacgc cgatgcgatg cattgttgcc ctcgctctgt
     2221 gcgttgccgt gggcggatcg atgtcgcctg cgccggcccg cgccgcggtt tcggccaggc
     2281 aggccgagca gctgaagacg gttctgacgc ctctcggcgc cgagcgcgcc ggcaacaggg
     2341 agcagacaat cccggcctgg acaggcggct acacgtctgt tgctgccagc tatcgcgaag
     2401 gcggtccgcg gcccgatccc tttgcgggcg agaagccgct gttttcgatc accgcgaaca
     2461 atcttgacgc ctatgccggg cggctgccgg agggcgccaa ggcgctgttc gggaaatatc
     2521 ccgactaccg gatggatatc tatccgacac atcgcagcgc cgccgcgccc gcctccgtct
     2581 atgccaacgt gctgttgaac gcgacgcgcg cgcacgccgc gccggaaggc atagcttatg
     2641 gagtcgcggg ggcggccggg ggagttccat ttccgattcc gcaaaacggt ttcgaggcga
     2701 tgtggaatca tctcctcgcc ttctgggggc cggcgcgcga actgcagctg aaaacctggg
     2761 tcgtatcggc cgatagcacg atcgaactcg cgagcggata ccgggaagtc gccgactttc
     2821 cctattacta tgcgagcgcg acacccgact cctatggagg ctactatttc aagacgctgc
     2881 atctcgaaga tgcgccggcg tccaaggtgg gcgaaggcta tctcgactgg cagcccatcg
     2941 atattgcacg gttcaatttc gatgcctggc gcgttctgcc gggcgagcac cgggtgcgcc
     3001 gcggtccggc gatttcctac gatacgcccg actccgacgc ggcgggattc gaaagcctcg
     3061 acgaatacta catctttttc ggcggtctcg accgcttcga tttcaagctg ctcggcaaga
     3121 aggaaatgta catcccctac aacaacaatc gtttctatac gcgtccgaca tccgagatcc
     3181 tgggaactcg ccacgccaat cccgacgatc tgcgctacga gctgcaccgc gtctgggtgg
     3241 tcgaaggcac actgaagccg ggagcccatg acgtcgtgtc ccgccgccgc ctctacctcg
     3301 acgaagacac ctggctcgcc gtctacagcg attcctggaa cgatcgcggc aagatctgga
     3361 agttcggcca gggtacgatg tatctcatgc ccgaaattcc cgccgtcata ctcggcagcc
     3421 agttcgtcta tgatctcgat ttgggcggtt acgttttcgg cttcgccttc aacgaccagc
     3481 cggaagccta caaggtgacc gcaccccact ccgcgagcct cttcacgccg gaatccatgg
     3541 ccgcgcacgc ggtgagatga gtttcatgac ttcggagcgg cgttggagcg gcgggcatgg
     3601 gcgttaagat aacacagccc gagcgacccc tcaccccttt cggagcggta gcgaggactc
     3661 gaatgtttcc cctctcccac aaggggagag gggactccgc gcgcttgttt tccgatgttg
     3721 atgcagcact ccgatttccc tctcccctcg tgggagaggg atgcagatac gtgcaatgat
     3781 ttctgctccg gcgcgggtga ggggcctctc gggctgcgtt gcctcagcgc ctacgctctt
     3841 gcgcaggccc tgagacaatt gagtgcgtaa attgaagccc aatcactctt ttgtgcgcag
     3901 aacttcgcct ccgggcgtga gcgcgtcgac agcgtgcgga aggacctgcg cgagcttttg
     3961 cgccagttcc tcggttgaca ttccagactt cgcggccagc tcgctcatcg tctggtcgcc
     4021 gaaggctcgg tgaacatctt gcggcgcaac cggcgcgttc ggcccttcat tgatccagga
     4081 attcaccgat ggcccgaggc cgttcgatct caactggttg acgacgccct gcacgccgcc
     4141 atggcgttcg atgagtccat tgaccaccgt cgccatttcc gcgcccacga ttccacccag
     4201 caatccgtcg aacagtccca tcatgtgctc ctgcgtaatc tggttgcgcg tctctgagcg
     4261 gttctgtggt tgcgtgccga accgcgcacc gcctggccat tacgccacct tctctccggc
     4321 ctgctcggcg taggcctttg cgcgcgcgcg gaattcctca cgggtgttcg agcgtgggaa
     4381 gggttctgcc ggaaccggaa cacccagcaa accgcaaatc ggctcccagc cctcacctgc
     4441 ccgccacacc agcagacgct ctttcggcac agtctccatg acccgcgcat tatgcgcgtt
     4501 gtaggcggca atcgatgcgt tccgatcgtt caggtcggcg ccccagcgtg ttcgaaacat
     4561 gtccgcgatc atggccttcc attccggcga cgcatggggg tggctctgaa tggaaggaaa
     4621 gatcgtctgg cttgcgctcg cccaccaatc ctcggcatcc cgaagcgaaa ggagcacgag
     4681 cgcgccagga taggcccgca tgagctccgg ccagaacgcg gctgccggcc aatccacggc
     4741 ggcggcgtag cccgcgaaga gcgcatccca atccgtcgct tcgccgagtg ccgcctgccg
     4801 ccagatcgcg gagtggccgg gatgctggaa cacttcgatc atgtgataac agggcgcgcc
     4861 gagcagccgc tcgagcgcga atttcagcga cgcggtgccc gtccgcccgc atcccgcacc
     4921 aatcacccgc aagctcatcg ccgtcccctg ccctgccgcg taagcctcgt agcatgggcg
     4981 cggcaaaatg ggcagcgcgt ctgcgtcacg ccgggctttt tcttttcacg gatgaccagc
     5041 ccacaagccc gatgcccaat gcgatgcaga tccaaccgaa tgcgtcgcca atgcggtcgt
     5101 agaatgttgc gccgcccctt tcgccaaccg gcaaatcgcc gatgagtgtg cgaaaccccg
     5161 cacgcgtccg gacggcggag atgatgcgtc cataacggtc gttgagcgtc agcaacccgt
     5221 cacgggccgt gcgcgccatg ggcacgccgt tctctacgct gcgcagcacg gcgacgcgcg
     5281 catggctcca atcgtccctg tcgaaatccc aggcgggcac agcgagcacc accggtttcg
     5341 tggcggtttc gtcgacgcgg atcatcgcct ggaaatccat gtccttgcag atctcgagtc
     5401 cggtgccatt ggagagcacc ctcggtcctg ggcccggttt gtagaaggcg gattcaagca
     5461 ccggcacgag gcggcgcttc atataggtga cgggccgcaa ccggccgggc acgaaagccc
     5521 aggaaacatt gcgttgcgcg ccgtcgagaa acgtattgaa gcccgccacg acagtggcgc
     5581 cggcatcgtt cgcagtggcg gcgagtttcg cctgcgcggc atttcgccag tccggtgcga
     5641 tctgcgcgat attttccggt agcacgatca attttgcttg cgcgccgttc agcttcgcga
     5701 tctgcgctgc ataggcatcg atatccttga gcgccgcggc cgtgtcgctg cgccgcgtcg
     5761 gccccgcgac gtcgttgctg tcgataagcg caacccggat cgagcccgtg ggcggcgtcg
     5821 acatccgcca gatcccgtag gccgcattgg cgacgaacag catcaatgca agcgcagcag
     5881 gcacgggatc gcgcgcgcgc agactgagcg cgatgcccgc cgaaatgaag ccgagcagaa
     5941 aggtgattcc cagatatccc acgagcgacg cactctggat caggaaagga gcgccgacct
     6001 cggcggcggc cggtgtcgcc accgaacctc ctccgctgtc gaaggacgca agaaaatcga
     6061 atgccgtcca cagcgacgcg aatgcgaaca tcgccaacac cgcgcccaac acaggctgca
     6121 cccgccgcgc gcccaaaacg gcgagcgcga atgtcagagc gggcgcgcat atgtagaaaa
     6181 tgagcacggg aaccggcagc agacccgcat aggcgcgcag gatgcttgtc gccccaagtg
     6241 cacaggctgc aaaacacgcg atgaaaaccg tccacggcct ggcgtcgccg aatgcgaacc
     6301 agaggatcgg aacgggcgcg atccaggcga gccaccacaa attgcccagg ccgaacgaga
     6361 aataaaatcc aaccgcgctc agcaaggcgc tggcgattgc gatcattctg ttacctccgg
     6421 cttgatcaaa gccaggacgg tgacatgtcc ggaagtgatt gccctccggc gcggtgcggg
     6481 ccgccgcgcg ctggcgttcc gtttccagca cttcgcatcc aaaagaatgt catcgcctgc
     6541 ttgcgcgcac aggcgccagg ataacacagc ccgagcgacc cctcacccgt ttcggatcgg
     6601 tagcgaggac tcgaatgttt cccctctccc gcagggggag agggaactcc acgcgttgat
     6661 ttcccgtcgt cgatgcagga ctccgattcc cctctcccct cgcgggagag ggatgcagat
     6721 acgtgcgatg atttcgcccc cggcgcgggt gaggggcctt cggcctgtgt tagcgcccat
     6781 gcgcgcaagc gggccatgac gtcggatcga tcaggtgagg ctggtataga cgagaccggc
     6841 caggcgttcc atctcggcgc cggaaaccgg accggccatc accgttgcca tgacttcgtc
     6901 ctgccatacg cagacatgca agcctttttc ctcgaacagg tcgaaccgcg gcgcgccgga
     6961 cgaacgtcgg agatagagcg tgaaaacacg gcccttgccg tcgcgatagc gcagatcgaa
     7021 agaccgcgtt ggcgtgttgt agacatccat gcccacgagg cggaatccca acttcgtcag
     7081 gtccggggcc ttcacctgcg tcgccagcac ccgcgtcatg acggcggctt cgacctccgc
     7141 atcgcgcgtc gaacggatgg gtatcacggt gtcgggcggc aagctctgcg tgcgcgcggc
     7201 aagcgcgtcc gccacgatgt cggcgcgcgc ggcgggcgcg aaatttctgt aggcgatgga
     7261 gccggcgaga accagcacga ccgtcgccgc catcgcagca atcgcccaat tcgtcctcga
     7321 gttccattgc cgacgtgcgc ctcggccttc gatcctctca atccactcgc gcggaagggg
     7381 ctcgttcaaa ccggcgccgt acagggaaac cagacgcgcc ttgtccgccc ggtagagcga
     7441 gagttggtgg cgcaacacag ggtccgcatc gacgatggat tgaacccgcg accggccaaa
     7501 atcgtcgagt tcgccgtcga tgtaggcgtg caggtcttcg tctctgagtt ctgtcgtcat
     7561 tgtcgcattt cccaggctcc agggacgtgg acggcgccag actggcgcct ctcgccttcg
     7621 agcgcacttc tcaatcgttc gcgtccgcgc gccagccgcg acatcaccgt tccgatcggg
     7681 atgccaagct cggtcgcgat ctcgcgataa ttcatgtcct ccagtccgac cagcaaaagc
     7741 acctgccggt gttcgacgct cagagtgtcc atggcgcgga cgaattcgaa cgccatggcg
     7801 ccgtcatgag ccggtgcgct gaacgaaagc tcatcggcca attcggcgat ctcctgttcg
     7861 tgcccccgcg tgcggtttcg gcgcagttcg tccacataca aattgtgcag gatcgaccgg
     7921 agccagctcg tcagccgctg cccatcccgg aggccttccc attgccgcag cgcccgctcg
     7981 atgcagtcct gcaccaggtc gtcggccagc gccgagttgc cgaccagggc tcgcgcgtaa
     8041 cggcgcagcg caggaagctg cgtaataagc gccttgccaa acgattcacg cctgctgccg
     8101 accacgctgc taccccgagc aggggctcgc atccgccccg cccgctcgta cccccctcgg
     8161 gcaggccggc ggctggaaag cccctaacca cgctaatatg acggacagca actgcaagtt
     8221 attcccccgt acccgccaat tggcaggaag ggatattggc ccgctttcca atacggtaaa
     8281 gaatggccgt cgggcatcgc cctgcccggc ccaagacccc gtagctcagc aggatagagc
     8341 ggcagattcc taatctgtag gtcggaggtt cgaatcctct cggggtcgcc atgtttcaga
     8401 agccgattgt agacacccag ttggctcaag tgaacgccgc tcaaaaatgc caagcatggg
     8461 gttacacgga tgcgcaggca tttggcggag gcttgagaca gtgcgaggag cgcaacgaat
     8521 acggctgcat ccgcacgctc gtaaccgtga cctatcaatg caccggagcc ggagcgccgc
     8581 attaagccga cttacagcta cgggaacaat gaacgaatcc agcatggttt cagtatgcgc
     8641 cggttggtaa cgccgcgccg ccctgaggaa cggcagcagc gccagacgca aatgatttcc
     8701 aaagtttcga ccagtggaag ccgtcggcca atgcaacgca gatcgttact gaagcgcgac
     8761 ggcttgcccg gaaatggtgc ccacgacacc atgcgcgact ttccagctca tgcagtcctt
     8821 gtagacactg tcacgcacta tctgctgcat gccggattca tagccgctgg ctgccgcgtc
     8881 gctggcacgc agttcggcca cagatgaaca atgtccgccc acgtcttgtg aatcggaagg
     8941 tgcggccgag ctcaccgttt cccgggactg ctgcgctgcc agccgcacat cggctgggcc
     9001 ggcgtcgaaa tctggtgtgg ttgcggtgtc atctgcctcg gcagcgtgat gtttctggta
     9061 aagagtgcgc gtcgtcgcgt aagggtcacg ggagattttt tcgatcttgt cggcggtggc
     9121 ggcgccacgg gcacgtttgt cgacaaacgt tgcaccggtg cggcccacgc tgacgaatat
     9181 gctggcgccg taggtaacgt atgtcagcgg atcgaatgcc gtatcgacaa gctttccggc
     9241 aagctcgcgc ggaacgctcg ggccgatcac aggcacatac agatatgggc cttcgccgac
     9301 gccatagtcg gccattgtat cggcgaagcc ggtttcgtgt tcgggaatgc cgatcttccg
     9361 tgcaacgtcg acaatgccgc cgataccggc ggtggaattg atcgtgaacc gcgcgagagt
     9421 ctccccgcct cgcacgatct tcccctgcaa cagatcgttc ccgaacgtca cgggcagatc
     9481 gatgttcaca agaaaattgt ggacgccgtc gcgcgccggc tccggaaccg catcgttata
     9541 ggagaccaca accggcctga tcacgttccg gtagagaatc tggtgcgatg cgaaggacga
     9601 tcgattggct gattcgtacg ggtcgtcaga cacgcccgcg cgggaggcat cactgctgca
     9661 acctgcaacg gccaacaccg tggcgagcgt cgccatacca ggcagaattc gcattcgcgt
     9721 cgcgcgcccc ttgtgctgtc agacaaggac cgaaatcgtc gcgccggtca agccatcgct
     9781 gcgaagcgca aggttgttct tttgtataga aggatgttcg tcaactcgtc gaaacagaag
     9841 ccggcactga tatcgcacac gcccaggctt tcacagagca gcggtgcggc acaatagcga
     9901 ataatgtccg gtttcaaacc gctccccgga ttgaacgaga ataccatgcc gcacaccttg
     9961 aatgcggcga ccccgttccg acggagcgtt acggactttt ctttcttgcc gaatcgatca
    10021 gtgtacggat gcgcctgacg aggtgctgca gatcgtaagg tttggccatg aagggctcgc
    10081 gctcgcacag gtccttcgcg gtagccgcct tcgccgcgtc acccgatgtc aggatgatcg
    10141 gaaggcccgg ctggttgatg cgaatccagt gcgccagccc gaagccgtcg agtttgcccg
    10201 gcatcaccac gtcgctgaac accagatcga tgacgaccgg gctgtgctgg atgatctcga
    10261 gagcttcatc ggcgttgccg gcttccagca ccttgaagcc gcattcctgg agatagtccg
    10321 acagcgcgat ccggatgaga acttcgtctt cgacgacgag aatagcggga gttctttcct
    10381 ccggatcggg aaatacaatc tcctgcacat tttcggtccg ggtcatgcaa ctcgccccaa
    10441 tgcttcggct gggcaacgtc cgaaggccga aaaagggtcc atcaagcgcc cgcgagaggc
    10501 tcgaaaccat ggccgcagtg gcgtatgctt cgcgcggtga agaaagagca aacaaacggg
    10561 cctgtgaaag aacctggacg ccggacggcc atcgacgccc tgctcgtctg ctggaaccgt
    10621 attgcggcga gcgcgttgag cgttggggtt gaaaggacgg cgaaggtggc gttcggggcg
    10681 agtgcgcgcc cattttcgga ccggtgcgcg gcgtggattt caccgaatcc ccttgaaaaa
    10741 gcggcagttt tgctgccggt cgcggttgcg tgccaggagc tcgcgaccgt ttgcgcgtgt
    10801 ccgccaattc acaaaagtta acgggagttt tttccaatgc ctgcgaaggc cgaagctgca
    10861 accgcgcaca agacgagaaa aagcgccgcc cgaccgtccg tgcccatgaa gggtacgagt
    10921 gaagcgggcg acaggttgaa cggtcacacc gcagaactgc atgaattgtt gcatgcgctg
    10981 caggcggtga agacgggcga tttttcggtg cgcatggcag gtcacgaaac cggcctcttc
    11041 ggcaagatcg ccgacacatt caacgagatc gtcagcaaca atgagcgcat ggccgaacag
    11101 ctcgaacatg tcgggcgcct cgtcggccgc gagggccaga cgaagcaccg cgtgaagctc
    11161 ggcagattga gcggcgcctg gggcgcgatg gaaagctcga tcaatgtgct gatcgacgat
    11221 cttctgtggc cgacgacgga agtgacgcgt gcgatctcgg ccgtggcgca gggcaacctc
    11281 ctgcaaacgg tgcggctcga cgtcgaaggc cggccgctca agggcgaatt cctgcaggcc
    11341 gcgactatcg tcaacaccat gatcaagcag cttggcgtct tcacctcgga ggtgacgcgc
    11401 gtcgcgcgcg aagtcggcac cgaaggcaag ctcggcggac aggcgcaggt gcgcgaggtg
    11461 acgggcgtct ggaaggactt gaccgagagc gtcaactcga tggcgagcaa cctgacggcg
    11521 caggtgcgca acatcgccga tgtcaccatc gccgtcgcga tgggcgatct ctccaagaag
    11581 atcaccgtcg acgtgcgggg cgaaatcctg cagctgaagg aagccatcaa tacgatggtc
    11641 gatcagctgc gctccttcgc ctcggaagtg acgcgcgtgg cgcgcgaggt tggcaccgac
    11701 ggcaagctcg gcggccaggc gatcgtgccc ggcgtggccg gcacgtggaa ggacctgacc
    11761 gactccgtca acgcgatgtg cggcaacctc accgaccagg tgcgcaacat cgcgcaggtg
    11821 acgaccgcag tcgcgcgcgg cgacctctcg cgcaaaatca ccgtggacgt gcgcggcgaa
    11881 atcctcgagc tgaaagacac catcaacacg atggtggacc agctcaacgc ctttgcctcg
    11941 gaagtgaccc gcgtcgcgcg cgaagtgggt accgaaggca agctcggcgg ccaggcgcag
    12001 gtgccgggcg tcgccggaac ctggaaggat ttgaccgaca ccgtcaacgt gatggcggcc
    12061 aacctcacag agcaggtgcg cggcatcgtg aaggtcgtga cggccgtcg
//
LOCUS       DASZMC010000411         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3725451,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000411 DASZMC010000000
VERSION     DASZMC010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3725451"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /locus_tag="VG274_08510"
     CDS             <1..2377
                     /locus_tag="VG274_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06735.1"
                     /translation="LYRSDDGGAHWMHVNKEKAFADWYASRLIVAPNDPDTVYTVGQS
                     IRRCTAGGKTCDIFKGAPGGDDYHYLWINPLHPDHMITASDQGAVVSVDGGRSWSSWY
                     NEPTGQFYHLAADNRFPYWIYSGQQDSGTVAIASRGDYGAITTRDWHPVGGDERDYDI
                     PDPADPGIVYDSGLGGRVTRWDARTGQVANISPWPESSYGKRPTLVRYHYLWVTPLVA
                     SRTGPAALYLGAQVLFRTQDQGAHWTAISGDLTGKRADAKNCSGDVKQADAMACGYGS
                     ISVVEPSQLRAEEIWVGTDDGLIQLTKDGGAHWSNVTPPGIRQWSKIAAIDLSVREPG
                     VAYAAVDGHRIDDFQPHVFVTRDSGKSWRDISHALPHDHFVSVVRADPVKSGLLYAGT
                     DAGTFVSIDDGAHWSPLGRNLPTAWVTDLLVHGDDLIAATQGRAIWLLDDVAPLRQTT
                     ANGEAAYLFAPAAAWRAHPDNNADTPLPPETPQGENPPAGAIIDYWLGAKPGTPVTLD
                     IRDAGGALVRHFASNAKPEEPRAKRYFATAWLKPPQRFETTAGFHRFVWNLRAERPPA
                     ISYSYSIAAVWGRGTPINPEGPYVLPGNYTVALGANGHTYTAPLHVAEDPRIKATQAD
                     LKASFDLSRRIAQLLTQTREGYGERKSVLKQLDALFPGEKTSSAEGDDTDTPAPEEKK
                     PADALHALGRQVRQKPAAGTPSFESVDTILTGVETDLEAVDAAPTPAQRQVVDDAASK
                     LADVQRRWANIKAGPLPALNAALARSARKPIVIPPPGALDTEPPDSGEDLP"
BASE COUNT          508 a          932 c          864 g          442 t
ORIGIN      
        1 gctctaccgc tccgacgacg gcggcgcgca ttggatgcac gtcaacaagg agaaggcgtt
       61 cgcggactgg tacgccagcc gtctcatcgt tgcgcccaac gatcccgaca ctgtgtatac
      121 cgtcggccaa tcgatccgcc gctgcaccgc cggcggcaag acctgcgaca tcttcaaggg
      181 cgcaccgggc ggcgacgact atcactacct gtggatcaat ccccttcatc ccgatcacat
      241 gatcaccgcg agcgatcagg gtgcggtggt gagcgtggac ggcggccgca gctggagcag
      301 ctggtacaat gagccgacgg ggcaattcta ccacctggct gccgacaacc ggtttcccta
      361 ctggatctat tccgggcagc aggattcggg gaccgtcgcg atcgcaagcc gcggcgacta
      421 cggcgcgatc accacgcgcg actggcatcc cgtcggcggc gacgaacgcg attacgacat
      481 tccggatccc gcggatcccg ggatcgtcta tgattccggt ctcggcgggc gcgtaaccag
      541 atgggacgcg cgcacggggc aggtggcgaa tatttcaccc tggccggaat cgagctacgg
      601 caagaggccg acgctcgttc gctaccacta tctctgggtg acgccgctcg ttgcctcgcg
      661 caccggtccc gcagcgctct atctcggcgc gcaggtgctt ttccgcacgc aggaccaggg
      721 agcgcactgg acggcgatca gcggcgatct caccggcaag cgcgccgatg cgaagaattg
      781 cagcggcgac gtcaagcagg ccgacgccat ggcgtgcggc tatggcagca tctcggtcgt
      841 cgaaccgtca cagcttcgcg cggaagagat ctgggtcggc acggacgatg gcctgatcca
      901 gctcacgaaa gacggcggcg cacactggag caacgtgaca ccgcccggaa ttcggcagtg
      961 gtcgaaaatc gcggcgatcg atctctccgt gcgcgagccc ggcgtcgctt atgcagcggt
     1021 cgacggtcat cgcatcgatg atttccagcc gcacgtgttt gtcacgcgcg actccggaaa
     1081 gagctggcgg gacatctcgc acgcccttcc ccacgatcat ttcgtgagtg tcgtgcgcgc
     1141 cgatccggtg aaatccggtt tgctgtacgc gggaacggat gccggcacct tcgtttccat
     1201 cgacgatggc gcgcactggt cgccgctcgg ccggaatctt ccgaccgcct gggtgacgga
     1261 ccttctcgtg catggggacg atctgatcgc ggcgacgcag ggccgcgcga tctggcttct
     1321 cgacgatgtc gcgccgctgc ggcagacaac ggcgaatggc gaagcagcgt atctcttcgc
     1381 gccggcggcc gcctggcgcg cgcatcccga caacaatgcc gatacgccgc tgccgccgga
     1441 aacaccgcaa ggcgagaatc ctcccgccgg cgcgatcatc gattattggc tcggtgcgaa
     1501 accaggcact ccggtgacgc tcgacattcg cgatgcgggc ggggcactgg tgcggcattt
     1561 cgcgagcaat gcgaagcccg aggagcccag ggcgaaacgc tatttcgcca ccgcctggct
     1621 gaaaccaccc caacggttcg aaacgacggc cggttttcat cgcttcgtct ggaatcttcg
     1681 cgccgaacgc ccgccggcca tcagctactc ctacagcatc gccgcggtgt ggggccgcgg
     1741 gacgccgata aacccggagg ggccttatgt gcttccgggg aactacaccg tggcgctcgg
     1801 cgcgaacggc cacacataca ccgcgccatt gcatgtcgcc gaagacccgc ggatcaaggc
     1861 gacgcaagcc gacctgaaag cgtcgttcga tctctcgcgg cgcattgcac aattgctgac
     1921 gcaaacacgc gagggctacg gggaaagaaa atccgtgctc aagcagcttg atgcgctgtt
     1981 tccaggcgag aaaacatcca gtgcggaagg cgacgacacc gacacgcccg cgcccgagga
     2041 gaagaaacca gccgacgcgc tccatgcgct cggccgtcag gtccggcaaa agccggccgc
     2101 gggcacaccg agtttcgaat cggtcgacac gatcctcacc ggggtcgaga ccgatctgga
     2161 ggccgtcgat gccgcgccga cgccggcgca gcgccaggtc gtcgatgacg cggcatcgaa
     2221 acttgcagac gttcagcgcc gatgggcgaa tattaaagcg ggtcccctgc ccgcgctgaa
     2281 cgcagcgctc gcgcgctcgg cgcgcaaacc catcgtcatc ccgccgcccg gcgcgctcga
     2341 caccgaacct ccggattcgg gagaggacct cccctgacgc cgaacgcgcg caccttgcgc
     2401 catcccgcgc cgcgtagcac agagctaggc aaacaaatat cctgtcccgg catttcctcg
     2461 gcaccggtgg tctgccgtcc acgcatccag atgctgaagt cgggcaccgg ttccgggaag
     2521 atgctgctct ctgagtgaag gcagacgctg cggcttggga ctgcagcgcc cgctccgcaa
     2581 ccccaccgcc gtcatggccc gccgcagagc gggccaccca gccacgagcg ctctccggtt
     2641 cgccatcgag aagattcttc ctctcgcttt cccctccgag cccgtttcat ctgggcggca
     2701 cgcttgcgcg ggccgtgaca cgtcagggga tgttgtcggt gacttt
//
LOCUS       DASZMC010000412         5479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2926775,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000412 DASZMC010000000
VERSION     DASZMC010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2926775"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VG274_08515"
     CDS             complement(<1..242)
                     /locus_tag="VG274_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HWE06736.1"
                     /translation="MPTRNVVLTQRQAKLVEKLVSSGRYQNASEVLRDGLRLIETRDT
                     EQKARLKALREAIQHGIDDMEAGRYRDFKSFDRLDD"
     gene            complement(307..951)
                     /locus_tag="VG274_08520"
     CDS             complement(307..951)
                     /locus_tag="VG274_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE06737.1"
                     /translation="MITISAFKWVPEFAQGKVRDLRARWALEEAGLPYKTRLLEQGDQ
                     DKPDYRALQPFGQVPILEENGLALFESGAIVLHIGERCETLLPKDAGARARATQWLVA
                     ALNSIEPFVMNLVLIDIFYTNEEWAKLRRPGAMEFVQKRLAALSTSLGDKPFLDGDRF
                     TAGDLMMTTVLRMLENTDVVTGDKRLAAYIERCTARPAFKRALDAQIGDFRKAA"
     gene            complement(948..1415)
                     /locus_tag="VG274_08525"
     CDS             complement(948..1415)
                     /locus_tag="VG274_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWE06738.1"
                     /translation="MTSLTLVRRIKARPSIVFDALTTAEGIACWWGPDDGPVLIAETD
                     PQVGGRFKVRFRMLDGSEHESSGEYFEVLKPERLSMSWRWTGGIEDPGESLIEIRLRA
                     IAEGTELTFTHSRLHDEESRRSHEQGWTGSLDKLERHFLPPKHSSTTPKEHIT"
     gene            complement(1412..1762)
                     /locus_tag="VG274_08530"
     CDS             complement(1412..1762)
                     /locus_tag="VG274_08530"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWE06739.1"
                     /translation="MNYNQRLGRTFAALVDPTRRAILARLEREDGASVSALARPFAIK
                     LPAVMKHLNVLDDAGLVTRSKIGRTVTVRLRPEPMREAMDWLRRYERFWSKSLDRLAA
                     YAENREAEARKKRS"
     gene            1874..2383
                     /locus_tag="VG274_08535"
     CDS             1874..2383
                     /locus_tag="VG274_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HWE06740.1"
                     /translation="MTRWVALLRAVNVGGTRKLLMSELAAMCRDAGFARVETYIASGN
                     IVFESKAAVPRVKMELEARLQAHARKPTGVVVRTASEIQAILDANPFPKADPRLTYAI
                     FLENPPPSDSLIHARGRKDEDMRLGEREILIHYPSGMGLSKLRIPAAKLGTARNMNTA
                     AKLVEIASR"
     gene            complement(2441..2737)
                     /locus_tag="VG274_08540"
     CDS             complement(2441..2737)
                     /locus_tag="VG274_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE06741.1"
                     /translation="MRLDLHIVDTLMRDLVSHDRAPSAFLLYLFLWRQTRGEGREVFG
                     ASLAELASDTGLSKSSVQNSLRRLKRRGLVSARRQGPTSACFYHVHEPWRRGGR"
     gene            2830..3192
                     /locus_tag="VG274_08545"
     CDS             2830..3192
                     /locus_tag="VG274_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE06742.1"
                     /translation="MITQLKFAGIPVREQERAVQFWTDKMGFKIVTDQPMGEQRWIEL
                     SIRTSATRIVLFTPPGHEDRIGSFFNGSLECDDVEATWRQLSAKGVEFVRPPKREDWG
                     TSTIFKDSEGNQFVMSSG"
     gene            complement(3330..3683)
                     /locus_tag="VG274_08550"
     CDS             complement(3330..3683)
                     /locus_tag="VG274_08550"
                     /inference="COORDINATES: protein motif:HMM:NF027074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HWE06743.1"
                     /translation="MRASRMPGKSLMRSTKTPASGKRASLPRAATITKQFAKDWEKLS
                     RTGRYDMRQLKEAMMLLIANDAPLGPEWLDHALKGEWADHRECHIGGDFLLIYQVEGN
                     QLNFVRAGTHAELFE"
     gene            complement(3619..3900)
                     /locus_tag="VG274_08555"
     CDS             complement(3619..3900)
                     /locus_tag="VG274_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HWE06744.1"
                     /translation="MASTAFVRARIDEKLKDEASEVLADFGLTVSDIVRMTLTRVARD
                     RAVPLELKVPNAESRAAMRESRALMKKHNARFSNAREIFDALDKDSRKR"
     gene            complement(3973..>5479)
                     /locus_tag="VG274_08560"
     CDS             complement(3973..>5479)
                     /locus_tag="VG274_08560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HWE06745.1"
                     /translation="RRVATGEARLLYLAPERLMGERMLEALRKLDVKLIAIDEAHCIS
                     QWGFAFRPDYRGLARLRNLFANVPIVALTATADAVTRVDVVEQLFAGHAESVVLGFDR
                     PNIRLAVKPKQDSKRQLLAFMKRHVGESGIVYCLSRKRTEQTAAYLEANGIRALPYHA
                     GMSKEAREANQNRFMTEPRLVMAATIAFGMGIDKADVRFVFHMDLPASLEAYYQEIGR
                     AGRDGATADAHLLLGYGDIRMRRLFIDEEDAGEDRKRREHQRLNALIGYCEAASCRRQ
                     ILLGYFGEASEPCGNCDACLDPVSLVDGTLPARQALSAIRRTGERYGAAHIVDVLRGN
                     ATEKLVAAGHDRIPTFAVGVERRKDEWHALIRQLVAGGWLAIDIAGYGGLSITEQGNS
                     LLRGAEHFHYRPAEPRLRRERVRAGDLPVDNSDAPLLGALKALRLRLAKERRLPAYLI
                     FSDRTLLDMAARRPRNAEEFAAVNGVGAAKLKEFAGPFLAAIAAHEESEGV"
BASE COUNT          982 a         1730 c         1689 g         1078 t
ORIGIN      
        1 tagtcgtcaa gccgatcgaa cgacttgaag tcgcggtaac gtcctgcttc catatcgtcg
       61 atcccgtgct ggatagcttc gcgcagcgct ttgagcctgg ctttctgctc cgtgtcccgc
      121 gtctcgatca ggcgcaggcc gtcgcgcagg acctcgctgg cattctggta gcggccggac
      181 gacacaagct tctccacgag cttcgcctgg cgctgtgtca gcaccacatt tcttgtcggc
      241 atcgcttgcg actccgttcg ttggcataat atgccatatg cggcggaatt tgcaagccgc
      301 gacctcctac gccgccttcc ggaaatcgcc gatctgcgca tcgagtgcgc gtttgaaggc
      361 gggccgcgcg gtgcagcgtt cgatataggc ggcgaggcgc ttgtcacctg tgacgacgtc
      421 ggtgttctcc agcatgcgca gcaccgtcgt catcatcagg tcgcctgcgg tgaagcggtc
      481 gccatcgagg aagggtttgt cgcccagcga tgtggagagg gcggccaggc gtttctgcac
      541 gaattccatc gcgcccgggc ggcgcagctt cgcccattcc tcgtttgtgt agaagatgtc
      601 gatcagaacg aggttcatga caaatggctc gatggagttg agggcggcga cgagccattg
      661 cgtggcgcgc gcgcgggcgc ccgcgtcctt cggcaggagc gtctcgcacc gctctccgat
      721 atgcagcaca atcgcgccgg actcgaacag cgcgaggccg ttttcctcca ggatcggcac
      781 ctggccgaag ggctggagtg cgcgatagtc cggtttgtcc tgatcgccct gttcgaggag
      841 gcgtgtcttg tagggcagtc cggcttcctc cagcgcccag cgcgcccgca gatcgcggac
      901 tttgccctgc gcaaactctg gaacccattt gaaagcggag atggtgatca tgtgatgtgc
      961 tcctttggtg ttgtcgagga atgtttgggc ggaagaaaat ggcgttccag cttgtcgagc
     1021 gatccggtcc agccttgctc gtggctgcgg cggctctctt cgtcatgcag gcgcgaatgc
     1081 gtgaatgtga gttcggtgcc ctcggcaatg gcgcgaagcc tgatctcgat cagcgattcg
     1141 ccgggatctt cgatgccgcc cgtccagcgc cagctcatgc tcagccgctc gggctttagc
     1201 acctcgaagt attcgccgct gctttcgtgc tcgctcccgt cgagcatgcg gaaacgcacc
     1261 ttgaacctgc cgccaacctg cgggtcggtt tccgcgatca gcaccgggcc gtcatcgggc
     1321 ccccaccagc aggcgattcc ttccgcagtg gtgagcgcat cgaagacaat cgatggccgc
     1381 gccttgatcc ggcgcacaag cgtcagactg gtcatgacct tttcttcctc gcttcggctt
     1441 cccggttttc ggcgtaggcc gcgagccgat cgaggctctt cgaccagaag cgttcgtagc
     1501 ggcgcagcca gtccatcgcc tctctcatgg gctccggccg gagccgtacc gtcaccgtgc
     1561 gcccgatttt cgacctggtg acaaggcctg cgtcgtcaag cacgttgaga tgcttcatca
     1621 ccgccggcag cttgatcgcg aagggccgcg ccagcgcgct gaccgacgcg ccatcttcgc
     1681 gctcgaggcg ggcgagaatc gcccgccttg tcggatcgac gagcgccgca aatgtccggc
     1741 cgagccgttg attatagttc accatgtagg aaagtatgga aaagaaggga ccgcctgtca
     1801 acccgctgcc gaccaattcc atagcggggc ggggcactgc gtgagtcccc aacgcgcgtg
     1861 atatgttcga ggcatgacaa ggtgggtggc tcttctccgc gctgttaacg ttggtggcac
     1921 gcgcaaactc ctcatgtcgg agcttgccgc catgtgccgc gacgccggct tcgcccgcgt
     1981 cgagacctac atcgcgagcg gaaacatcgt ttttgaaagc aaagccgcag tcccaagggt
     2041 caagatggaa cttgaagccc gtttgcaagc ccacgcccgc aaaccaacag gggttgtcgt
     2101 gcgcaccgcg tccgagatac aggccattct cgacgccaat ccatttccga aagccgaccc
     2161 gagactcacc tatgcaatat ttctcgaaaa tccgccgcct tccgactccc tgatccatgc
     2221 gcgggggcgg aaagacgaag acatgcgcct cggagaacga gagatattaa tacattaccc
     2281 gagcggcatg ggcctttcga aattgagaat tcctgccgcg aagctgggca cggcgcgcaa
     2341 catgaacacc gccgccaaac tcgtggagat cgcgtcgaga tgacagatcg ctcgtcttct
     2401 cttgtgggaa tcgtgacgcc tcgcctttca gctcctcctc ctatcttccc ccccgccgcc
     2461 acggctcgtg cacatggtag aagcacgcgc tggtcgggcc ttgccggcgc gcggaaacga
     2521 ggccgcggcg cttgagccgg cgcagcgagt tctgcaccga cgatttcgac agtccggtat
     2581 cggacgccag ttccgcaagg cttgcgccaa acacttcgcg gccttcgcca cgcgtttgcc
     2641 gccacaggaa gagatagaga agaaacgccg agggcgcccg gtcgtggctg accaggtcgc
     2701 gcatcagcgt gtcgacgatg tgtagatcga gccgcatggc aaccgttact atagtaaagg
     2761 ctgttcggct tgtctcccct gaatcctgcc gccaaatttg ccgcacattt cgagaggcgg
     2821 agacgcgacg tgatcacaca gctgaaattc gccggcatac cggtgcgcga gcaggaacgc
     2881 gccgtgcagt tttggaccga caagatgggg ttcaaaatcg tcaccgatca gccgatgggc
     2941 gagcagcgct ggatcgagct ttcgatccgc accagcgcca cgcgcattgt gctgttcacc
     3001 ccgccgggcc acgaagatcg tatcggctcg ttcttcaacg gctccttgga gtgcgacgac
     3061 gtggaagcaa cctggcgcca gctctcggcg aagggcgtgg aattcgtccg gccgcccaag
     3121 cgggaggact ggggcacgtc gacgatcttc aaggattcgg aggggaacca gttcgtcatg
     3181 agctcggggt agttcctcaa gaactggttt aagcaatatt cgcgcaagcc gggcatccaa
     3241 cgttccgcag ttcccgcgac gttctcccgc gtagctatcc ggtgccgatt gagatcgctc
     3301 ttgtcattgc ttcactgtcg cgtgagcgat tattcgaaca gctcggcgtg cgtgccggcc
     3361 cgcacgaagt tgagctgatt gccttcgact tgatagatca ggagaaagtc accgccgata
     3421 tggcattcgc gatggtcggc ccactcgcct ttcaaggcat gatccagcca ttcgggacca
     3481 agcggtgcgt cattggcgat cagcaacatc atggcttcct tgagttggcg catgtcgtat
     3541 cggccagttc gcgaaagctt ctcccagtcc ttggcaaatt gtttcgtaat ggtcgctgcg
     3601 cgaggaagtg atgcccgctt accgcttgcg ggagtctttg tcgagcgcat caaagatttc
     3661 cctggcattc gagaagcgcg cattgtgctt cttcatgagc gcacgcgact cgcgcatggc
     3721 ggcgcgagat tctgcgttcg gaactttcag ctccagggga acggcgcggt ccctggcaac
     3781 tctggtcaaa gtcatgcgca cgatatccga aactgtcagc ccgaaatccg cgagcacttc
     3841 gctcgcctcg tccttcagct tttcgtcaat tcgcgcccgc acgaaagcag tcgaggccat
     3901 gatcaagatc tccagacgcg gtgtttgtag ctcaattgag caacgtggtc aagaaccgtt
     3961 tacccgttcg tctcaaaccc cctcgctttc ttcatgcgcg gcaatcgccg cgaggaacgg
     4021 gccggcgaat tccttgagct tggcggcgcc cacgccgttg accgcggcga attcctccgc
     4081 gttgcgcgga cgccgcgccg ccatgtcgag aagcgtgcgg tcggaaaaaa tgaggtaggc
     4141 cggcagccgc cgctctttcg cgagcctgag ccgcaacgcc ttcaaggctc caagcagcgg
     4201 agcgtcgctg ttgtcgaccg gcaggtcgcc agcgcgaaca cgttcgcggc ggaggcgcgg
     4261 ctcggcgggg cgataatgaa aatgttccgc gccgcgcagg agcgaattgc cctgctcggt
     4321 gatcgacagc ccgccatatc cggcgatgtc gatcgcgagc cagccgccgg cgacgagctg
     4381 gcggatcagc gcgtgccact cgtccttccg ccgctccacg ccgacggcga aggtgggaat
     4441 gcggtcatgt ccggcagcga cgagtttctc ggtcgcgttg ccgcgcagca cgtcgacgat
     4501 atgcgccgcg ccgtagcgct cgccggtgcg cctgattgcg gagagcgcct gccgcgcggg
     4561 cagcgtgccg tcgacgagcg acaccggatc gaggcaggcg tcgcaattgc cgcaaggttc
     4621 gctcgcctct ccgaaatagc cgagcagtat ctgccgccgg cagctcgccg cctcgcaata
     4681 gccgatcaac gcattgaggc gctggtgttc gcgccgcttg cggtcttcgc cggcgtcctc
     4741 ctcgtcgatg aacagccgcc gcatgcggat gtcgccgtag ccgaggagca gatgcgcatc
     4801 cgccgtcgcg ccgtcgcgcc cggcgcggcc gatctcctga taataggctt cgaggctcgc
     4861 gggcagatcc atgtggaaca cgaagcgcac atccgccttg tcgatgccca tgccgaaggc
     4921 gatggtcgcc gccatcacga gacggggctc ggtcatgaag cggttctggt tggcttcacg
     4981 tgcctccttg ctcattcccg cgtgataggg cagggcgcgg atgccgttcg cttcgaggta
     5041 tgcagccgtc tgttccgtgc gtttgcgcga caggcaatag acgatgccgc tttcgcccac
     5101 gtggcgcttc ataaaggcga gcagctgccg cttggaatcc tgcttcggtt tcaccgccag
     5161 ccggatattg ggccggtcga agccgaggac gacactttct gcgtggccgg caaaaagctg
     5221 ttcgacgaca tcgacgcgcg tgaccgcatc ggccgtcgcc gtcaatgcga cgatcggaac
     5281 attcgcgaag agattgcgca gccgcgccag cccgcgataa tcggggcgga acgcgaagcc
     5341 ccattgcgag atgcaatgcg cttcgtcgat ggcgatcagt ttcacgtcaa gcttccggag
     5401 ggcttccagc atccgttcgc ccatcagccg ctcgggcgcg agatagagca gccgcgcttc
     5461 gcctgtcgcc acgcgccgc
//
LOCUS       DASZMC010000413         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2092315,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000413 DASZMC010000000
VERSION     DASZMC010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092315"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..606
                     /locus_tag="VG274_08565"
     CDS             79..606
                     /locus_tag="VG274_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06746.1"
                     /translation="MQERFSASIGRIFAAMVPFGFALLGAMIANFPVSFTGGFLPTPL
                     FALMPVYFWGLLRPDLMPPWVAFAIGLTEDLLSGGPPGIWAASFVASYAFVDRQRDSL
                     AGLAGLGAILGFAVALLVALGTAYAIVALYYLRIPPPAPVMADLAVNVLWYIPALMLM
                     NFAQHHLIGAIRSGL"
     gene            606..>2107
                     /gene="mrdA"
                     /locus_tag="VG274_08570"
     CDS             606..>2107
                     /gene="mrdA"
                     /locus_tag="VG274_08570"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWE06747.1"
                     /translation="MPLFDGKDKSRYATFTRRTLMTGGAVGAVLGALGVRLYQLQILE
                     GEQFRTRAEQNSVSARLVAPLRGRIFDRFGVELANNRRNYRVLIIPEQALEGVEAALD
                     TVGRIILLTDRDRARILRDVAQNKKFVPSTVAENLTWEEFARVNMHLPYLSGIQPDAG
                     ETRDYPFGDELSHVLGYVAAVSPKDEQRDDDPVLELPGFRIGKRGIEKAYDAKVRGNA
                     GVSRVEVNAYGRVIRELSRDPGSPGADVWLTIDHELQKFVVQRLGAESVACTVMDVQT
                     GDVLALASTPGYDPNLFNVGINATQWQTLLNDDHRPLTDKALAGLYPPGSTFKPAVAL
                     SALDAGAITPDFAVNCSGSLALGNYVFHCWKKHGHGHIELHRAIAESCDVFFYNVARR
                     LGIDGIEAGAHKLGFGRPTNIEIPGERAGVIPGRAWKEATFGAPWLEGETLNAGIGQG
                     YVLVTPLQLCTMAARIASGNEVSPRIVRVVGKDAEPRIAPKRLAFSDAALA"
BASE COUNT          336 a          696 c          679 g          396 t
ORIGIN      
        1 gctccgcttc gctgcgcaaa ccacctcccc cacgatgggg gaggaaaggc cctctccccc
       61 gaagggggag agggggtaat gcaggagagg ttcagcgctt cgatcggccg tattttcgcg
      121 gcgatggtgc cgtttggctt cgcccttctc ggagcgatga tcgcgaactt tccggtgtcg
      181 ttcaccggcg gatttctgcc cacaccactc tttgcgctca tgccggtcta tttctggggc
      241 ctgctgcggc cggacctgat gccgccctgg gtcgcctttg ccatcggcct cacggaagat
      301 ctgctttcgg gcggcccgcc aggcatctgg gcggcgtctt tcgtcgcctc ctatgcgttc
      361 gtcgaccggc agcgtgattc gcttgcgggc ctggcgggac tcggcgccat tctcggcttt
      421 gccgtcgcgc tactggtggc tctcggcacg gcctacgcga tcgtcgcact ctattatctg
      481 cgcattccgc cgcccgcgcc ggtaatggcc gatctcgccg tcaatgttct ctggtacatt
      541 ccggcgctca tgctgatgaa tttcgcgcag caccatctga tcggcgcgat ccggagtggc
      601 ctctgatgcc tctcttcgac ggcaaggaca agagccgtta cgcgacattc acccgccgca
      661 cgctgatgac cggcggcgcg gtcggcgctg tgttgggagc gctcggcgtc cggctctacc
      721 agctccagat tctggagggc gagcagttcc gcaccagggc cgagcagaac agcgtcagcg
      781 cgcggctggt cgcgccgctt cgcggccgca tcttcgaccg cttcggcgtc gagctggcca
      841 acaaccgccg caactatcgc gtcctgatca ttcccgagca ggcgctggaa ggcgtcgagg
      901 cggcgctcga cacggttggc cgcatcatcc ttctcaccga ccgcgaccgc gcgcgaatcc
      961 tgcgcgacgt ggcgcaaaac aagaagttcg ttccttcgac ggtcgcggag aatctgacct
     1021 gggaagagtt tgcgcgcgtc aacatgcatc ttccctatct ctccggcatc cagcccgatg
     1081 ccggcgagac gcgcgactat cccttcggcg acgagctttc gcatgtgctg ggttatgttg
     1141 cggcagtgtc gcccaaggac gagcagcgcg acgacgatcc ggttctcgag cttccaggct
     1201 tccgcatcgg caagcgcggc atagagaagg cctatgacgc gaaggtgcgc ggcaacgcgg
     1261 gcgtgagccg cgtggaggtg aacgcctatg gccgggtgat ccgcgaactg agccgcgatc
     1321 cgggatcgcc gggcgccgat gtctggctga cgatcgacca cgaactgcag aaattcgtgg
     1381 tgcagcgtct gggcgcggag agcgtggcat gcacagtgat ggacgtgcag acgggcgacg
     1441 tgctcgcgct cgcctcgaca ccgggctacg accccaacct cttcaatgtc ggcatcaacg
     1501 cgacgcaatg gcagaccctg ctcaacgacg atcatcggcc gcttaccgac aaggcgctgg
     1561 ctggcctcta cccacctggt tcgacattca agcccgccgt cgcactttcg gcgctcgatg
     1621 cgggcgccat cacaccggat tttgctgtca attgctcggg ttcgctggcg ctcggcaatt
     1681 acgtgttcca ctgctggaag aagcacggac acggccatat cgagctgcat cgcgcgattg
     1741 ccgagtcctg cgacgtcttc ttctacaatg tggcgcgccg cctcggcatc gacggcattg
     1801 aggcaggcgc gcacaagctg gggttcgggc ggccgaccaa catcgagatt cccggcgaac
     1861 gcgcgggcgt gataccgggg cgcgcctgga aagaggcgac cttcggcgcc ccctggctcg
     1921 aaggtgagac gctaaacgcc ggcatcgggc agggctatgt gctggtcacg ccgcttcagc
     1981 tctgcaccat ggccgcgcgc atcgcgagcg gcaatgaggt gagcccacgc atcgtgcgcg
     2041 tggtggggaa ggatgccgag ccgcgtatcg cgccgaagcg cctcgccttc tccgacgccg
     2101 ccttggc
//
LOCUS       DASZMC010000414         6668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_986114,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000414 DASZMC010000000
VERSION     DASZMC010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986114"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VG274_08575"
     CDS             complement(<1..1392)
                     /locus_tag="VG274_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HWE06748.1"
                     /translation="MAKRTAAGRMPLALLPELMRAMLRRAMRGLRVMWRRSWFYRQLL
                     KGPLTDRIVFHPYDALPRRLEDADAVLHGKFRFNGETVEVREGSVFDKAPPSLRWAEA
                     LHGFAWLPPLSAAGGEAARTLATNLISQWLKRNARYAEPNCSAHVLARRLIHIFAHGR
                     LVLTNSDMLWRSKVFVSLREQSRLLGRIAGEAPEGMPRFEAATALALCGACLADPRRL
                     ELGLAMVEAEIARQILPDGGHITRSPEDLVHAYRLITMVMDALTATSQAIPQELRAAH
                     DRIAPMIRFFRHGDGALALFNGGQECDTRMIAGLLARDEVRGQPFAFAPHSGYQRIAS
                     GRTLLVMDCGTAPPGGYSTEAHAGCLAFELSTNTHRIVVNCGAANDARRAVWDTALRA
                     TAAHSTLTLADTSTGAILQSGWARDFLGPRLLGAVTAVETSRVETPQGWMVEASHDGY
                     VAAFGVRHERQVTL"
     gene            complement(1396..2055)
                     /gene="rpe"
                     /locus_tag="VG274_08580"
     CDS             complement(1396..2055)
                     /gene="rpe"
                     /locus_tag="VG274_08580"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWE06749.1"
                     /translation="MTASIRIAPSILSADFARLGAEIEAIEKAGADLIHVDVMDGHFV
                     PNITIGPAVVKAIKPHAKKPLDVHLMISPVDPYIEDFANAGADGITVHPEAGPHVHRT
                     LQLIRSLGKKPGVVLNPGTPVDALDNVIDLVDLVLIMSVNPGFGGQSFITSQLKKIEA
                     AAKLIAQTGRDIALEVDGGITPETAKQAIAAGANVLVAGTSVFRGGAAHYAANIAALR
                     G"
     gene            complement(2102..3538)
                     /locus_tag="VG274_08585"
     CDS             complement(2102..3538)
                     /locus_tag="VG274_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06750.1"
                     /translation="MSDSQQVSEGLVRAWRTRIVLPDAPLRFVRNEVAAVLALTPILM
                     LAAFCNGFPIIFYDTGAYILEGFGHVFIAERSPVYSLFLKYAGGPESLWYVAFLQCLI
                     TAFVVVELARALKAQLSLWAFLVIGLILTIATGLPWYAAQIEPDCFVGVAAIAMYLLA
                     FHRRDLSFARSGLLVVCAGLGAATHSSHIGLAIGLLAVLVAMRFALLFFRNLDVPRPS
                     LLTPAMSCALAFATVFACNYAFTRHVFFSKAGSVFLEARMMQDGLIKPVLDTDCPAAG
                     YKICPYRDNLPPRADAYLWEEKTSPFSRLGGFRKLEAESTHLAALSLMRYPLSNAAWA
                     AIDTMLEFFAVATGDGIEPQEWVLNREFEHTIPQQMNGYTHAYQQRGDLWFLPLNVLH
                     VPVAILSIFGVFYLMRRAMQERDWNTAVLPAFVLIALLGNAFICGVFSGPHSRYQSRL
                     VWLPTLVLLLQVSAGTGIFPRRRAETAT"
     gene            3713..4132
                     /gene="sdhC"
                     /locus_tag="VG274_08590"
     CDS             3713..4132
                     /gene="sdhC"
                     /locus_tag="VG274_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWE06751.1"
                     /translation="MAENPQKAARPRPLSPFVTIYRWPVTMATSIVHRITGAGLALGT
                     IVLAWWLIAIASGPESYQIFTGIAASLIGQIALFGFAWSLAYHLLNGIRHLAWDTGYG
                     FDVKTADATGWFVIVLSVLLAVGLFALAYTGHGGFNT"
     gene            4129..4524
                     /gene="sdhD"
                     /locus_tag="VG274_08595"
     CDS             4129..4524
                     /gene="sdhD"
                     /locus_tag="VG274_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWE06752.1"
                     /translation="MTMETPLHKVQGLGSSHSGVKHFWRQRMTAAALIPLAVWFAWAV
                     LGLVGGSEAQVLGYLTRSAFGLPLNAILMGAFIVIALYHMVLGLQVIIDDYVHSPGGK
                     IFLMLFMRAFALAIAAASLFALFRIAGPV"
     gene            4524..6311
                     /gene="sdhA"
                     /locus_tag="VG274_08600"
     CDS             4524..6311
                     /gene="sdhA"
                     /locus_tag="VG274_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWE06753.1"
                     /translation="MTAYPITDHTFDVVVVGAGGAGLRATLECAARGLKTACITKVFP
                     TRSHTVAAQGGISAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIEYLCKSAPDAVYEL
                     EHYGVPFSRTAEGKIYQRAFGGMTSHYGKGIAQRTAAAADRTGHAILHTLYGQCVKHE
                     VNFFVEYFALDLIVNDGAVVGIMAWNLDDGTIHRFRAHRVILATGGYGRIYQSCTGAH
                     TQTGDGNAMVLRAGFPLQDMEFVQFHPTGIFGAGVLITEGVRGEGGYLTNSEGERFME
                     RYAPNAKDLASRDVVSRAMTIEIHEGRGVGPNKDHINLHLSHLDPKVIHERLPGISES
                     ARIFAGVDVTKEPIPVLPTVHYNMGGIPCAYTGDALTLTAGNPDTVVHGLMAVGEAAC
                     VSVHGANRLGSNSLIDLVVFGKAAGMQAAKSLGEGMTHQDLPKDAGDAALARLDKFRN
                     ANGNTPTSQMRATMQKAMQDDAAVFRTGETLKNGQTRIHDLYATLGDVKVSDRSMIWN
                     SDLVETLEYQNLVQQAIVTIDGAVNREESRGAHAREDFPNRDDQNWMKHTLAWLDSET
                     GKVRIDYRPVHTYTLTNDVEYIPPKARTY"
     gene            complement(6364..6591)
                     /locus_tag="VG274_08605"
     CDS             complement(6364..6591)
                     /locus_tag="VG274_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06754.1"
                     /translation="MNVRIRSLIKRIGYGLALVFLTFLIGGAIIPLDIAFILATVILT
                     YLGFAFTPVTRNKIMADLRNKVRRLAALTFR"
BASE COUNT         1365 a         2114 c         2002 g         1187 t
ORIGIN      
        1 aagcgtcacc tgccgctcat gacgcacacc gaaggcggcg acatagccat cgtgactcgc
       61 ttcgaccatc cagccctgcg gcgtttccac ccggctcgtt tcgacggccg tgacggcgcc
      121 tagcaaacgc ggaccgagaa aatcgcgcgc ccacccggat tgcaggatcg caccggtcga
      181 tgtgtcggcg agcgtcagcg tcgaatgcgc cgcggtcgcg cgcaacgccg tgtcccatac
      241 cgcacggcgc gcatcgttgg ccgcgccgca attgacgacg atacggtgcg tgttcgtgct
      301 caactcgaat gccagacacc ctgcatgcgc ctcggtcgaa tagccgcccg gcggcgcggt
      361 gccgcaatcc atgacaagga gcgtccgtcc agaggcgatg cgctgataac ccgaatgcgg
      421 tgcgaacgcg aaaggctggc cgcgcacttc atcgcgcgcc agcagaccgg cgatcatgcg
      481 cgtgtcgcat tcctgcccgc cgttgaacag cgccagcgcg ccatcaccat ggcgaaagaa
      541 ccggatcatc ggcgcgatgc gatcatgcgc ggcgcggagt tcctgcggta tcgcctgtga
      601 cgtcgccgtc agcgcgtcca tcaccatcgt gatcagacga taggcatgca cgaggtcttc
      661 cggcgagcgc gtgatgtggc cgccatcggg aagaatttgg cgcgcgatct ccgcctcgac
      721 catcgcaagg ccaagttcga gacgtcgcgg atcggcaaga caggcgccgc acaacgcaag
      781 tgccgtcgcg gcttcgaaac gcggcatgcc ttcgggcgct tcaccggcga tgcgccccag
      841 caaacgcgat tgttcgcgta gcgaaacgaa caccttcgag cgccacagca tgtcggaatt
      901 cgtcagcaca agacggccat gcgcgaagat atggatgagc cgccgcgcca gaacatgggc
      961 ggagcaattc ggctccgcat aacgcgcgtt acgcttcagc cattgggaaa tcagatttgt
     1021 cgcaagcgtg cgggccgcct cgccgcctgc ggcagacaac ggcggcagcc aggcgaagcc
     1081 gtgcaacgcc tccgcccacc gcagtgacgg tggggcctta tcgaagaccg aaccttctcg
     1141 gacctcgacc gtctcgccgt tgaaacgaaa cttgccatgc agcaccgcgt ccgcgtcttc
     1201 gagacgccgt ggcagcgcgt cataaggatg gaaaacgata cgatcggtca gcggtccctt
     1261 gagaagctgg cgatagaacc agcttcgccg ccacatgacg cgcaacccac gcatcgcccg
     1321 gcgcagcatg gcgcgcatca gctccggcag gagagcgagc ggcatgcgtc cggctgccgt
     1381 gcgctttgcc acggcctatc ctcgcaacgc tgcgatgttg gcggcgtaat gtgccgcgcc
     1441 gcccctgaac accgacgtgc ccgcgacgag cacgttcgcg ccggccgcga tggcctgctt
     1501 cgccgtttcc ggcgtgatgc cgccatcgac ttctagcgcg atgtctcgac cggtctgcgc
     1561 gatcagcttc gccgccgctt cgatcttctt caattggctc gtgatgaatg actgcccacc
     1621 aaagccggga ttgacgctca tgatcagtac cagatcgaca agatcgataa cgttgtccaa
     1681 tgcatcgacc ggcgttcccg gattgagaac cacaccaggc ttcttcccga gcgagcggat
     1741 aagctggagc gtgcggtgaa cgtgcggacc ggcttccgga tgaaccgtga tcccgtcggc
     1801 acccgcattc gcgaaatcct caatgtaagg atctaccggc gagatcatca gatgcacatc
     1861 gagcggcttc ttcgcatgcg gcttgatcgc tttgacgaca gcgggaccga tggtgatgtt
     1921 cggcacgaaa tgcccgtcca tcacgtcgac atggatgaga tcggcgcccg ccttctcaat
     1981 tgcttcgatc tcggcaccaa ggcgcgcgaa atcggcggaa aggatggacg gagcgatgcg
     2041 gatggatgcg gtcatggcgc ctcatggtgt gccggattcg accggcctcc gcaaggtaaa
     2101 gtcaggtggc ggtctcggcg cgtcggcgcg gaaaaatccc ggttcccgcg gaaacttgca
     2161 gcagcaggac cagcgtcggc agccagacga gtcgcgactg gtaacgtgaa tgcggaccgg
     2221 agaagacgcc gcagatgaaa gcgttgccca gcaaggcgat caacacaaag gctgggagga
     2281 ccgccgtgtt ccagtcgcgc tcctgcatcg cacgccgcat cagatagaac acgccgaata
     2341 tcgacaggat cgcgaccggc acgtgcagca cgttcagcgg caggaaccag agatcgccac
     2401 gctgctgata ggcgtgggtg taaccgttca tctgttgcgg gatcgtatgc tcgaactcgc
     2461 gatttagcac ccactcctgc ggctcgatgc cgtcgccggt cgcgaccgcg aagaattcca
     2521 gcatcgtatc gatcgccgcc catgcggcat tcgacagcgg atagcgcatg aggctgagcg
     2581 ccgcgagatg cgtcgactcc gcttccagct tgcggaagcc accgagccgc gagaacgggc
     2641 tggtcttctc ctcccagaga tacgcgtccg cgcgcggcgg aagattgtct ctatacggac
     2701 agattttgta accggctgca ggacaatcgg tatccagcac gggcttgatc aacccatcct
     2761 gcatcatgcg cgcttcaagg aaaaccgacc ccgccttgct gaagaagacg tgccgggtga
     2821 aggcatagtt gcaggcgaac acggtcgcaa atgcgagcgc acaactcatc gcgggcgtca
     2881 gaagcgacgg ccggggaacg tcgagattgc ggaaaaacag cagcgcgaac cgcatggcga
     2941 caagaaccgc aagcaatccg attgccaatc caatatgcga agagtgcgtc gcagcgccca
     3001 atcctgcgca aaccacaagc agtccactgc gtgcgaagct gagatccctg cgatggaacg
     3061 caagaaggta catcgcaatc gcagcaacac cgacgaaaca gtccggctcg atctgcgcgg
     3121 cgtaccacgg aagccccgtg gcaatcgtca ggatcaatcc gataacgaga aatgcccaga
     3181 gcgacaattg cgctttgagc gcgcgcgcca actccacaac gacgaatgcg gttatcaggc
     3241 attgaaggaa cgcgacatac cagagactct ccggcccgcc ggcatatttc aggaacagcg
     3301 aatagaccgg cgagcgctcg gcgatgaata catgtccaaa accttcgagg atgtaggcgc
     3361 cggtatcgta aaaaatgatc gggaacccgt tgcagaacgc ggccagcatc aggatcggcg
     3421 tcaacgccag aacggcggcg acctcattgc ggacaaaacg caatggcgcg tcaggcagca
     3481 cgatgcgtgt gcgccacgcg cgcaccaaac cctcgctcac ctgttgcgaa tcgctcattc
     3541 cccaacccac ttgcccggcc cggaagactt ctatgtgccg gatcgcgccg caaagcgcaa
     3601 agcggcagtt aggccatggt gaaattggga aactccgccg tttcggttga cttgggggtg
     3661 tcgaaaccta cactccggcc cagttcgcgt ttaccccgtt tcgagggatt tcatggcgga
     3721 aaacccgcaa aaagcagctc gcccacgccc cctttcgcca ttcgtgacga tctatcgctg
     3781 gccggtgacg atggcgacgt cgatcgtcca tcgcatcacc ggtgcaggcc tcgctctcgg
     3841 caccattgtc ctcgcctggt ggcttatcgc catcgcgagt ggacctgaga gctatcagat
     3901 cttcacaggt atcgcggcga gcttgatcgg gcagatcgct ttgtttggat ttgcgtggtc
     3961 gctcgcctat cacctgctga atggcattcg ccatctcgcc tgggacaccg gctacggctt
     4021 cgacgtgaag acggccgatg cgacgggatg gtttgtcatc gtcctttcgg tgctgctggc
     4081 cgtcggcctg tttgccctcg cttacaccgg ccatggcggg ttcaacacat gaccatggaa
     4141 accccgcttc acaaggttca gggccttggc tcgtctcatt ctggcgtgaa acatttctgg
     4201 cgccagcgta tgacggcggc cgcgctgatc ccgctcgcgg tctggttcgc ctgggctgtg
     4261 cttggtcttg tcggtggcag cgaggcgcag gtgctgggct atctcacgcg ctccgccttc
     4321 ggcctgccgc tgaacgcgat cctgatgggc gcgttcatcg tcatcgcgct ctatcacatg
     4381 gtgctcggcc ttcaggtcat catcgacgac tacgtgcaca gccccggtgg aaagattttc
     4441 ctgatgctgt ttatgcgcgc cttcgcgctc gctatcgccg ctgcgtcgct cttcgccctc
     4501 ttccgcatcg ctggacccgt ttgatgaccg cttatccgat taccgatcat accttcgacg
     4561 tcgtcgttgt tggcgccggc ggcgcgggct tacgcgccac gctcgaatgc gccgcacgtg
     4621 gtctgaagac ggcctgcatc accaaagtct ttccgacacg cagccatacc gtcgccgcgc
     4681 aaggtggcat ctctgccgcg ctgggcaaca tgggcgagga cgattggcgc tggcacatgt
     4741 acgacaccgt gaaagggtcg gactggctcg gtgatcagga cgccatcgaa tacctctgca
     4801 agagcgcgcc ggacgcggtc tacgagcttg agcattatgg cgtgccgttc tcacgcacgg
     4861 cagaaggcaa aatctatcag cgcgccttcg gcggtatgac cagccattac ggtaagggta
     4921 tcgcgcagcg caccgctgct gctgccgacc gcaccggcca cgcaatcctg cacacgcttt
     4981 acggccagtg cgtgaagcac gaggtcaatt tcttcgtcga gtatttcgcg ctcgacctga
     5041 tcgtgaatga cggcgcggtt gtgggcatca tggcgtggaa cctggatgac ggcacgattc
     5101 accgcttccg cgcgcatcgc gtgatcctgg cgaccggcgg ttatggccgc atctatcaga
     5161 gctgcaccgg cgcacacacg cagaccggcg acggcaatgc gatggtcttg cgcgcgggat
     5221 tcccgctgca ggacatggag ttcgtgcagt tccacccgac cggcatcttc ggcgcaggtg
     5281 tgctgatcac cgagggcgtg cgcggcgaag gcggctatct caccaactcc gaaggcgagc
     5341 gcttcatgga gcgctatgcg cccaacgcga aagacctcgc ctcgcgcgac gtcgtctcgc
     5401 gcgcgatgac catcgagatc cacgaaggcc gcggcgtcgg cccgaacaaa gaccacatca
     5461 atctgcacct gtcgcatctt gacccgaagg tgatccacga gcgcttgccc ggcatctcgg
     5521 aaagcgcgcg catcttcgct ggtgtggacg tgaccaagga gccgatcccg gttctgccga
     5581 cggtgcatta caacatgggc ggcatcccct gcgcctatac gggcgatgcg ctgacgctga
     5641 ccgccggtaa tcccgacacg gtggtgcatg gccttatggc cgtcggcgaa gcggcgtgcg
     5701 tttccgttca tggtgcaaat cgcctaggct ccaactcgct gatcgacctt gtcgttttcg
     5761 gtaaggcagc cggtatgcag gcggcaaagt cgcttggcga aggcatgacg catcaggacc
     5821 tgccgaagga tgcgggcgac gcagcgctgg cgcggctcga caaattccgc aacgcgaacg
     5881 gcaatacgcc aacctcgcaa atgcgcgcga cgatgcagaa ggcgatgcag gacgacgccg
     5941 ccgtgttccg caccggcgag acactcaaga acggtcagac gcgcatccac gatctctacg
     6001 cgacgctcgg cgacgtgaag gtcagcgacc ggtcgatgat ctggaattcc gatctcgtcg
     6061 agacgttgga ataccagaac ctcgtccagc aggcgatcgt caccatcgac ggcgcggtca
     6121 accgcgagga aagccgcggc gcccatgcgc gcgaagactt ccccaaccgc gacgaccaga
     6181 actggatgaa gcacaccctc gcctggctcg acagcgagac cggcaaggtg cggatcgact
     6241 accgcccggt gcacacctac accctcacca acgacgtcga atacatcccg ccgaaggcgc
     6301 ggacgtatta gactactggc aacttcgcgg cgaccaagtg cgcaatttcg tatgaaagac
     6361 gctctaccga aaggtgagcg ccgcgagcct acggactttg tttcgaaggt ccgccattat
     6421 tttgttcctg gtcaccggcg tgaaggcaaa gccgagatag gtgagaatca ccgtggccag
     6481 aatgaaagcg atatccagag gtatgattgc gccgccgatt aggaacgtca gaaataccag
     6541 cgcaagtccg tagcctattc gttttatgag tgagcgaatt cgtacgttca cctaacgacc
     6601 tcgtgcattc aatttgcggc gatggcttca ggttcttcgt agagaaattc cactcgccaa
     6661 cctccgca
//
LOCUS       DASZMC010000415         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3204638,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000415 DASZMC010000000
VERSION     DASZMC010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3204638"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(889..>1792)
                     /locus_tag="VG274_08610"
     CDS             complement(889..>1792)
                     /locus_tag="VG274_08610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWE06755.1"
                     /translation="ANGNGHGDGGRIFASPLARRIAADKGIDLHQVRGTGPNGRVVQA
                     DVLSFQPQAATGAPAARSAAPDSPRVAGATEVIPLNKMRTAIAAALQRSKQQIPHFYE
                     TIDIDVEALSSLRGVLNAQLEKQKIKLSLADFISKAVCVALKQNPAVNATFDGTQITR
                     HADIHLGIAVAVPDGLIVPVLKHADKMGLKELRERTGPLVERARSGKQKQDEVNGGTF
                     TISSLGAFGIREFSAIINPPQVAILAVGSAEKRAVVRNDQIVARTMMSVTLSADHRVV
                     DGATAAEFLRTLKSLLEEPGMMLV"
BASE COUNT          323 a          515 c          629 g          325 t
ORIGIN      
        1 gcgaagtagc ccccaagaaa cgcaatcacc gcaaacgcga tcgtcgtcat ccatgactgc
       61 tgatgcatgt cggcgatgtt acgcccgatc gctgccacgg caagcgggta aaggaaggga
      121 attcgcggaa atcacgtacc gcagggccgg agccagactt tgaaccttcg accgagtttg
      181 cgtcgcgccg cgaatgtcgt cagcggaccg gcaccgcgca ggggcgcgcc gcgccgcatt
      241 ccgggcagcc gccgggcgag ccgcggaggt cgtagccgca ggaaaggcag cggccagtgc
      301 gcaggcgctt ccttcggcgg gatattcggg ccggcgccaa gaggcagggg attgtgagta
      361 gtagcaagat caaccaaaac ggtacttcga ggtgggtagt gcgtttgacg atctgtcttc
      421 cactggggta gctccaattg tcggtcgctg tgtcataccc caggccggca aagtgatgag
      481 cgggtgcgtg ccaaaagatc attggcggcg cgtcaatgaa tagaccagtg ttgaggtcgt
      541 agtatccaaa tctggaataa tcccatccct cccgggtcga gttaaccgag attggattcc
      601 acgttggagc gtggctgcgg aagtgcgtct cgacgtccaa gtccaggccg tgcggatgag
      661 tggccagcgc gtatcgcgtg tcagcatggg tgagcaggct atctcgtcgg agacgcggag
      721 cgagcggccc cataagacaa gactccccgc acaaaacaga accgagagaa tgacgatgag
      781 tcgccgtgcc atcggcggtg ggtgtttgtg ggccgggcgt acggcccgcc tgcggtgagc
      841 cgatcgaacc gtacgcccct gcgggagtcg ggcacacgcc gtgtgcccct acacgagcat
      901 catccccggc tcctcgagca gcgacttgag cgtgcggagg aattcggcgg cggtggcgcc
      961 atccacgacg cggtggtcgg ctgagagcgt caccgacatc atcgtgcggg cgacgatctg
     1021 atcgttgcgg acgacggcgc gcttctcggc gctgccgacg gcgaggatcg ccacctgggg
     1081 gggattgatg atggcggaaa attcgcggat gccgaaggcg ccgaggctgc tgatggtgaa
     1141 cgtgccgccg ttcacttcgt cctgcttttg cttgccggag cgggcgcgct cgaccagcgg
     1201 gccggtgcgc tcgcgcagtt ccttcaggcc cattttgtcg gcgtgcttga ggacggggac
     1261 gatcaagcca tcgggaaccg cgaccgcaat gcccaggtga atgtcggcgt ggcgggtgat
     1321 ctgagtgccg tcgaaggtgg cattgactgc cgggttttgc ttgagcgcga cgcagacggc
     1381 tttggagatg aagtccgcca gcgacagttt gatcttctgc ttttcgagct gcgcgttcag
     1441 cacgccgcgg agcgaggaaa gcgcttccac gtcgatgtcg attgtctcgt agaagtgggg
     1501 aatctgctgc ttggaccgct gcagcgccgc tgcgatggcc gtccgcattt tgttcagcgg
     1561 gatgacttcg gttgcgcctg cgacgcgggg ggaatccggg gcggcgctgc gcgctgccgg
     1621 cgccccggtg gcagcttgcg gttggaatga aagaacgtcg gcctgcacaa cgcgtccgtt
     1681 cggaccggtg ccgcgaactt gatggagatc gatgcccttg tcggcggcaa tgcgccgggc
     1741 gaggggtgac gcgaaaatcc ggcccccgtc accgtgacca ttgccattcg cg
//
LOCUS       DASZMC010000416         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3479695,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000416 DASZMC010000000
VERSION     DASZMC010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3479695"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VG274_08615"
     CDS             complement(<1..800)
                     /locus_tag="VG274_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWE06756.1"
                     /translation="MKHILKELEERRSRARLGGGEARIKAQHDRGKLTARERIELLLD
                     ANSFEEFDMFVEHRNTDFGAEKNRIPGDGVVTGWGTINGRMAYVFAKDFTVFGGSLSE
                     THALKICKIQDMAMQNQAPIVGLLDAGGARIQEGVASLAGYGEVFKRNVLASGVVPQI
                     SVIMGPCAGGDVYSPAMTDFIFMVRDTSYMFITGPDVVKTVTQEDVTPEELGGASAHT
                     VKSGVADNAFDNDVEALLAARDFIDFLPANNEAGVPEWPTQDDSDRND"
     gene            complement(1022..2098)
                     /locus_tag="VG274_08620"
     CDS             complement(1022..2098)
                     /locus_tag="VG274_08620"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWE06757.1"
                     /translation="MTPVEASIAAAPLAGAANVPVESDDDGARLDRWFRRHFPALAHH
                     ALEKLLRTGQVRLDGKRAKSGDRISTGQIIRLPPQVQNFHEAVDARSTNSQPVPAQSS
                     EQARIFAESLVIHKDSAVLVLNKPSGLASQGGSGIGEHVDGLLGFLAFGKKQRPRLVH
                     RLDRDTSGVLVVARTVPAAAALSESLRRRDAAKIYWALTKGVPSPARGTIRSALAKQA
                     AGRDEKIAEVECDAEGAKSAITDYAVVERAGPDYAWVAVRPLTGRMHQIRVHLASLGT
                     PVVGDFKYGGAAARGLGAIENRLHLHARAIDIAHPDGGRLKISAPLQAHMLKTWKLLG
                     FDPDEDAFPAGGAAKSKRRKRKPR"
     regulatory      2111..2188
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2318..>3388
                     /locus_tag="VG274_08625"
     CDS             2318..>3388
                     /locus_tag="VG274_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWE06758.1"
                     /translation="MANVWLGSALWLGLALAASLISIRIAVSVALVEIVVGAFAGNLI
                     GLHITDWVNFLAGFGAILLTFLAGTEIDPRIVRKHFWSSMTIGIMGFFAPYLGVLAYA
                     HWGLHWPWPQAQIAGISLSTTSVAVVYAVMIETGFNKTELGKIILAACFINDLGTVLA
                     LGIVFAHYDITLLVFAAATLAVLLLLAFYAPRLVKSLGHRVSEPQTKFVTLILFGLGG
                     LASVAGSEAVLPAYLVGMALAPTFLAERELAQRMRTIAFAILTPFYFLKAGSLVEWHA
                     VVGSAGLIAIFLGIKMATKFIGILPLTAYHEFDRREGLYTTLLMSTGLTFGSISALFG
                     LTNKIIDQSQYTILVTAVVASAV"
BASE COUNT          581 a         1077 c         1035 g          695 t
ORIGIN      
        1 tcatcgttgc ggtcgctgtc gtcctgcgtc ggccattccg gcaccccggc ctcattattg
       61 gcgggcagga agtcgatgaa gtcgcgcgcg gcgagcagcg cctcgacgtc gttgtcgaag
      121 gcgttgtcgg cgacgcccga cttgacggtg tgcgcgctgg cgccgcccag ctcttcgggc
      181 gtcacgtctt cctgcgtgac cgtcttcacc acgtcgggac cggtgatgaa catgtaggac
      241 gtgtcgcgca ccatgaagat gaaatcggtc atcgccggcg aatagacatc gcccccggcg
      301 cagggtccca tgatcaccga tatttgcggc acgacgccgc tcgccagcac gtttcgcttg
      361 aagacttctc catagccggc gagactcgca accccctcct ggatgcgcgc gccgcccgca
      421 tcgagcagcc cgacgatcgg cgcctggttc tgcatcgcca tgtcctggat cttgcagatc
      481 ttcagggcgt gcgtttcgga gagagagccg ccgaaaaccg tgaagtcctt ggcaaagaca
      541 taagccatgc ggccgttgat cgtgccccag ccggtcacga ccccgtcgcc cgggattcta
      601 tttttctccg cgccgaaatc cgtgttgcgg tgttcgacga acatgtcgaa ctcttcgaag
      661 gaattggcgt cgagaagaag ttcgatccgc tcgcgggcgg tgagcttgcc gcggtcgtgc
      721 tgggccttga tccgcgcctc cccgccgccc agccgggcgc gggaccgccg ctcttcgagc
      781 tccttgagga tatgcttcat cgaatcgctc cgcccgatac ttgcaaacgt gacactttgg
      841 ctaaccaaat ccaacggccg gagctgtgga gatgcgcgaa atctcacgac aatgaggaca
      901 aagaacacga agatgatcgt gcgccccgag atggcgggca tcttgcccgc atgccagcgc
      961 gcgagaccgc gcgcggtccc aggattcttt gtgctcttgg tgttctttgt ggtggaccgc
     1021 tttatcgagg cttgcgcttc cggcgtttcg acttcgcggc gcctccggcc ggaaaagcat
     1081 cctcatcggg atcgaagccg aggagcttcc acgtcttcag catatgcgct tgaagaggcg
     1141 ccgaaatctt caatcgcccg ccatcgggat gggcaatgtc gatcgcgcgc gcatgaagat
     1201 gaagcctgtt ttcgatcgca cccagtccac gtgccgcagc acctccgtat ttgaaatccc
     1261 cgaccaccgg cgtgccgagg gaagcgaggt gcacgcggat ctggtgcatg cgtccggtta
     1321 aggggcgaac ggcaacccag gcgtagtccg gccccgccct ttcgacgacc gcgtaatcgg
     1381 taatcgcgga tttcgcgccc tcggcatcgc actcgacttc ggcgatcttc tcgtcgcgtc
     1441 ccgcagcctg cttggcgagc gccgacctga tcgttccgcg cgccggactg ggcacacctt
     1501 tcgtcaacgc ccaatagatc ttcgcggcat cgcggcgccg gagtgattcg ctcagcgcgg
     1561 cggccgcggg caccgtgcgg gcaacgacga gaacgcccga cgtgtcgcga tcgagccggt
     1621 ggacaagtct ggggcgctgc ttttttccaa aggccagaaa ccccagcaaa ccatcgacat
     1681 gttcgccgat gccgctgccg ccctggctcg ccaggccgct cggcttgttg agaaccagca
     1741 ccgcggaatc cttatgaatg acaaggctct ccgcgaatat cctcgcctgt tcagaagact
     1801 gtgcgggaac gggctgtgaa tttgtggatc gggcatccac agcttcatga aagttctgaa
     1861 cctgtggcgg caagcggatg atctggcctg tggatatccg gtcgccggac ttcgcccgtt
     1921 tgccgtccag ccgcacctgg cctgtgcgca acagcttttc gagtgcgtga tgcgccagcg
     1981 ccgggaaatg gcgccggaac cagcgatcga ggcgggcgcc gtcatcgtcg ctctcgacgg
     2041 gcacattcgc tgcgccagcc agcggcgctg cggcaattga cgcttccacg ggagtcactt
     2101 aatcgtttcc gtctcgaaag gggatggggt tcccctttaa ccgccagaaa gctcaagaag
     2161 ccagacggct gatgacccct gccgcacaga agctgtggtg ggagtgtgtc ttgccggccg
     2221 aagacgctcg ccccctgcgt ctttcgacaa ggtcagggcg agggaacatc tccctgaccc
     2281 tgagcctgtc gaagggtgaa gtaacgggag aacgcgcatg gcaaatgtct ggctgggatc
     2341 ggcgctctgg ctcggccttg ctttggccgc ttcgctgatt tcgatccgga tcgcggtttc
     2401 ggtggcgctg gtcgagatcg tggtgggcgc ttttgccgga aacctcatcg gcctgcacat
     2461 tacagattgg gtcaatttcc tcgcaggctt cggcgccatc ctgctgacgt ttctggccgg
     2521 aacggagatc gatccgcgca tcgtcaggaa gcatttctgg tcgagcatga ccatcgggat
     2581 catgggcttc ttcgcgccgt atctgggcgt gctggcctat gcgcattggg gccttcactg
     2641 gccatggccg caggcgcaga tcgccggcat ctcgctttcc acgacctcgg tcgcggttgt
     2701 ttacgcggtg atgatcgaga cgggattcaa caagacggaa ctcggcaaga tcattctcgc
     2761 cgcctgcttc atcaatgatc tgggcacggt gctcgcgctc ggcatcgtgt tcgcgcatta
     2821 cgacatcacg ctgctcgtct ttgccgcggc gacgcttgcg gttctgctgc tgctggcgtt
     2881 ctacgcgccg cgtctggtca agagtctcgg acaccgggtc agcgagccgc agacgaaatt
     2941 tgttacgctg atcctcttcg ggctcggcgg gctggccagc gtggccggca gcgaagccgt
     3001 gcttcccgcc tatctcgtcg gaatggcgct cgcaccgacg ttccttgcgg agcgcgaact
     3061 ggcacagcgc atgcgcacca tcgcattcgc gattctgaca ccgttttatt ttctcaaggc
     3121 cggctcgctg gtcgaatggc atgccgtcgt cggatcggcg ggcctcatag ccatttttct
     3181 cggcataaag atggcaacga agttcatcgg tatcctgccg ctcacggcct atcacgaatt
     3241 cgaccggcgc gaaggcctct acacgacgct gctcatgtcc acgggcctga ccttcggctc
     3301 gatctcggcg ctgttcggcc tgaccaacaa gatcatcgac cagtcgcaat acacgatcct
     3361 cgtcaccgcc gtggtcgcga gcgccgtc
//
LOCUS       DASZMC010000417         5084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3205344,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000417 DASZMC010000000
VERSION     DASZMC010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3205344"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..550)
                     /locus_tag="VG274_08630"
     CDS             complement(245..550)
                     /locus_tag="VG274_08630"
                     /inference="COORDINATES: protein motif:HMM:NF018545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HWE06759.1"
                     /translation="MNEATRTEIEAAVFRRLVQHLRARTDVQNMDLMILAGFCRNCLG
                     DWYREAAAEKGIALGKDESREIVYGMKPSEWKDRFQTETTPEQKAAFEAAQKTHSHG"
     gene            734..2155
                     /gene="pyk"
                     /locus_tag="VG274_08635"
     CDS             734..2155
                     /gene="pyk"
                     /locus_tag="VG274_08635"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWE06760.1"
                     /translation="MRRTRKTKIVATLGPASSSPEQLKSLFEAGADVFRINMSHTSRE
                     RLAELHGRVRALEREDGRPIGILADLQGPKLRLGQLAGGARELRTGTRVGLILDARSD
                     DSEAIPIPHPEVFAALRQGDGLLIDDGKIRLKIESIGPKAADAVVVTGGAVRDRKGVN
                     LPDTVLPIPALTAKDRSDLDAALNLGVDWVALSFVQRPDDVAELRKLVSGKAAVLAKI
                     EKPKALEWLSEILDLTDALMVARGDLGVELALETVPIRQKEIVSAARRLGKPVVVATQ
                     MLESMIEAAVPTRAEVSDVATAIFDGADAVMLSAETAAGKHPVEAVAMMDRIACAVET
                     GPIFQSFMDAQRCAPEETTPDAIMAAVHQVTHTMHARAIVSWTKSGSTGLRAARERPE
                     APIIALTPSLDTARRLSLAWGLHCVLTEDAHDLDDVVERAAHFAQAEGFAKAGERIVV
                     TAGVPLGTPGATNLLRVAFVGGR"
     gene            complement(2357..2932)
                     /locus_tag="VG274_08640"
     CDS             complement(2357..2932)
                     /locus_tag="VG274_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06761.1"
                     /translation="MRTLLFAVSLAAFSVAGASPHRTQQPPVAHAANPQVDMLFGQLA
                     KVGSDEEAKPIEEQILTLFLQSGSASVDLLMARTAASLAAGDLDTARSLLQVVTEIAP
                     EYAEGWHQRGKLQAVGGDDEGAIVSLQKAVTLNPRQFAALTELGSILVEYGDKRDALK
                     VLRKALAIDRHFANVDHEVQQLSREVEGERI"
     gene            complement(3069..3194)
                     /gene="ykgO"
                     /locus_tag="VG274_08645"
     CDS             complement(3069..3194)
                     /gene="ykgO"
                     /locus_tag="VG274_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HWE06762.1"
                     /translation="MKIRNSLRSLKKRDKDCRVIRRRGRTYVINKKNPRFKARQG"
     gene            3338..4597
                     /locus_tag="VG274_08650"
     CDS             3338..4597
                     /locus_tag="VG274_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HWE06763.1"
                     /translation="MFAQCGNLVAAALALAIGVAIPVEACRAQTAPPTIVAPADQDAK
                     FRSFLAEFRTTALNTGIDPQIYDSAMAGIARNPRVETLNLEQPEFAKPIWDYLDGAVS
                     SERIANGQALLASWGAVLATIEQRFGVQKEILVAIWGIESDYGRQMGSFDMFEALATL
                     AYDGPRSDFGRRELLDALRMEQQERLAPAQMTSSWAGAFGQTQFLPSTFLRYAVDGDG
                     DGRRDLWHSPSDALASAANLLAQSGWERGAPWGYEVTLPAGFSYELADLDKTSTISTW
                     RNMNVRAASGLALPRSEALSSIYLPAGARGPAFIVYDNFRTVLKYNNAASYALAVCTL
                     GDRLKNYTPIMAAWPRDEMPLTRDERLALQADLKRLGFDPGVLDGVMGRQGRAALRAY
                     QKARGLVPDGFATEHLLERMESELLAR"
     gene            complement(4601..>5084)
                     /locus_tag="VG274_08655"
     CDS             complement(4601..>5084)
                     /locus_tag="VG274_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE06764.1"
                     /translation="TGIGLVASSLAALFAQLVIVQRFNISVRVLIRWGAIVALAANLL
                     FAIGHQFGPLVFALILSGLGFGLVRPGYAAAASLAVDPDEQGAVAGLVGATNGAGFIF
                     GPMIATALYRWSPSAPYLFGAALMAALYAYALLSPHLRNAGACADAQGETAETQVPNA
                     "
BASE COUNT          920 a         1642 c         1605 g          917 t
ORIGIN      
        1 gcggtcgagt cgtctcaggc gcacgacctg ccgcatgatc ctggtatcaa agccctgtcc
       61 cttcgcttcg gaatagactt ccctgatatc cgcggtcagc gccgctttct cctcttcgag
      121 ccgctcgacg cgctcgacga gtgatttcaa ctgatccttc gcgaagtttt tggccatgtc
      181 ccgcgcacct tattcaaagg cgcggaccct actccgcgcg gttccgcgcg cgcaatgcgg
      241 gcgctcagcc gtggctgtgg gttttttgcg cggcttcaaa cgcagctttt tgttccggcg
      301 tcgtttccgt ctggaagcgg tctttccatt cggacggctt catgccgtag acgatctctc
      361 gcgactcgtc cttgcccagt gcaattcctt tttccgcggc cgcctcgcgg taccaatcgc
      421 cgagacaatt gcggcaaaag ccggccagga tcatcagatc catgttctgc acgtcggtcc
      481 gcgcgcgaag atgctgcacc agacggcgga acacggcggc ctcgatttcc gtgcgcgtag
      541 cttcgttcat gaccaacctc cggtgttcgc aatcataccc gatcgggcgc cgttcacatt
      601 gacagaatgg atcgcatggg cgaaaccctc gggccaagcc atgcgtccgg ttcctgccct
      661 tttcttgacc aggctcgcga gcggcgctgc ggctgccgca ttcaatccca cgccattcct
      721 gccggactga accatgcgcc gcacgcgcaa gacaaaaatc gtggcgacgc tcggtccggc
      781 gtcctcctcg cccgaacagc tcaagtcgct gttcgaggcc ggcgccgacg tgttccgcat
      841 caacatgagc cacacttcgc gcgagcgtct cgccgaactg cacggccgcg tgcgcgcgct
      901 cgagcgcgaa gacgggcgtc ccatcggaat tcttgccgac ctgcagggac cgaagctgcg
      961 tctcggccaa ctcgcgggcg gcgcacggga actcaggacg ggaacgcgcg tgggattgat
     1021 cctggatgcg aggtccgacg acagcgaggc aattccgatt ccgcatcccg aagtcttcgc
     1081 agctttgcgt cagggggacg gtctgctcat cgacgacggc aagatacgtc tgaagatcga
     1141 aagcatcggg cccaaagccg ccgacgccgt cgttgtgacg ggcggcgcgg tcagggaccg
     1201 caaaggcgtc aaccttcccg acaccgtcct gccgattccc gccctgacgg caaaggaccg
     1261 cagcgatctc gatgcggcac tcaatctggg tgtggactgg gtcgcgcttt ccttcgtgca
     1321 gcggcccgac gacgtcgcgg aactgagaaa gctcgtttcg ggaaaggccg ccgtgctcgc
     1381 caagatcgag aagcccaagg cactggaatg gctctccgag attctcgatc tcaccgatgc
     1441 gctgatggtg gcgcgcggcg atctgggcgt ggagctggcg ctcgagaccg tgcccatccg
     1501 gcagaaggag atcgtgagtg cggcgcgccg cctgggcaaa cccgtcgttg tcgcaacgca
     1561 gatgctggaa tcgatgatcg aagcggcggt accgacacgg gcggaggtct ccgacgtggc
     1621 gaccgcaatt ttcgacggcg ccgacgcggt gatgctgtcg gcggaaacgg cggcgggaaa
     1681 gcatccggtg gaagcggtgg cgatgatgga caggatcgcc tgcgcggtcg agaccggccc
     1741 gatttttcag tccttcatgg atgcgcaacg ttgcgcaccg gaagaaacga ctcccgacgc
     1801 aatcatggcc gccgtccatc aggtcacgca taccatgcat gcgcgcgcga tcgtgagctg
     1861 gacgaaatcg ggatcgacgg gactgcgcgc cgcccgcgag cgccccgagg cgccgatcat
     1921 cgcgctgacg ccgagcctcg acacggcgcg ccggctttca ctcgcctggg gccttcactg
     1981 cgtgctcacg gaagacgccc atgatctcga cgacgtcgtg gagcgcgccg cgcatttcgc
     2041 ccaggcggaa ggtttcgcga aggccggcga gcgcatcgtc gtcaccgcgg gcgtcccgct
     2101 cggcactccc ggcgcgacaa atcttctccg cgtcgctttc gtggggggaa ggtagcgcta
     2161 cgtacgcatg ctcttcgcgc cgggagagcg tcggtttttt cccaaacatc attgagttca
     2221 aattgcgcaa ctcatgtatt gtcatggccc tgcttgacag ggccacccag cggcaagcga
     2281 cgtggggttc gaactgggtg gcccgcttgc gcgggccatg acaacatatt ttcgttgggt
     2341 gggtttgcta accgccctaa atcctctcgc cctcgacctc gcgggagagc tgctgcacct
     2401 cgtggtcgac attggcgaaa tggcggtcga ttgcgagtgc cttgcgcagc accttgagcg
     2461 cgtcgcgctt gtcgccatat tccaccagaa tggaccccag ttcggtcagc gcggcgaact
     2521 ggcgcggatt gagcgtcacc gccttctgca gactgacgat cgcgccttcg tcatcgccgc
     2581 cgacggcctg aagctttccg cgctgatgcc agccttcggc gtattccggc gcaatttcgg
     2641 tgaccacctg caaaaggctt cgcgccgtat cgagatcgcc tgccgccagc gaagccgcgg
     2701 tgcgcgccat caaaagatcg acactggcgc ttcccgactg gaggaagagc gtgagaatct
     2761 gttcctcgat cggttttgcc tcctcgtccg agccgacttt ggcgagctgc ccgaaaagca
     2821 tgtcaacctg cgggtttgca gcatgtgcaa caggcggttg ctgcgtgcga tgcggtgacg
     2881 cgcccgcgac ggaaaaggcg gcaagagaca ctgcaaacag cagcgtacgc atcgaaaacc
     2941 ctatcccgaa acggcaacct gcaccatcga atacgtgcgg cggaccggcg cgttcccgtg
     3001 gaaagccgca gtcgcgagga atattgcact ggcgccggat attgcgcccg accgaaaccg
     3061 gcggggggtt aaccctggcg ggccttgaag cgcgggtttt tcttgttgat cacataggtc
     3121 cggccgcggc ggcggatgac gcggcagtcc ttgtcgcgct tcttgagcga gcgcagcgag
     3181 ttgcggatct tcatggtacg ccttcgagag tccgggcggg cacgagggcg ccgccgaaga
     3241 ggcgcggaaa ctattggcgg ccaccggtcc tgtcaacctg cgcccgacac ctgcgcccgc
     3301 gcctgtgcta agaggctcaa gattcgggga tgaaaacgtg tttgcgcaat gtggaaatct
     3361 ggtggcggcg gcgctcgccc tggcaatcgg tgtggccata ccggtcgagg cgtgccgcgc
     3421 gcagacggcg ccgcccacga tcgtggcgcc cgccgaccag gacgccaaat tccgctcgtt
     3481 ccttgccgaa tttcgcacca ccgctctcaa caccggaatc gatccgcaaa tttacgattc
     3541 cgcgatggcc ggaattgcgc gcaatccgcg agtcgaaacg ctcaatctcg aacagccgga
     3601 attcgcaaaa ccgatctggg attatcttga cggcgccgtt tcctccgagc ggatcgccaa
     3661 cggccaggcg ctgcttgctt cctggggcgc ggtgctcgct actatcgagc agcgcttcgg
     3721 cgtgcagaag gaaattctcg tcgcgatctg gggcatcgaa agcgactatg gcaggcagat
     3781 gggcagcttc gacatgttcg aggcgctcgc gacgctcgcc tatgacggtc cgcgaagcga
     3841 tttcggccgg cgcgaactgc tcgacgcctt gcggatggaa cagcaggaac ggctcgcccc
     3901 cgcacagatg acgtcgtcct gggcgggcgc gtttggacag acgcagttcc tgccgtccac
     3961 ctttcttcgc tacgcggtcg atggcgacgg cgacggaagg cgcgatctct ggcattcgcc
     4021 gtccgatgcg ctcgcctcgg ccgccaatct tcttgcgcaa tcgggctggg agcgcggcgc
     4081 gccctggggc tatgaagtta cgctgcccgc gggattttct tacgagcttg ccgatctcga
     4141 caagaccagc accatctcga cgtggcgcaa catgaacgtg cgggcggcga gtggtctggc
     4201 gctgccccga agcgaagctc tctcgtcgat ctatcttccc gccggcgcgc gcggtcccgc
     4261 cttcatcgtc tacgacaatt tcaggaccgt attgaaatac aacaacgccg catcctatgc
     4321 gctcgccgta tgcacgcttg gcgaccggtt gaaaaactat acgccgatca tggcggcatg
     4381 gccccgcgat gagatgcccc tcacgcgaga cgagcggctt gctctccagg cggacctcaa
     4441 gcgactcggt ttcgatccgg gtgtgctcga tggtgtaatg ggccgccagg gccgcgccgc
     4501 gttgcgcgcc tatcagaagg cgcgcggact cgttcccgac ggcttcgcaa ccgaacacct
     4561 gctcgaacgc atggaaagcg aactcctggc gcgctgaaat tcaagcattc ggcacctgcg
     4621 tctccgccgt ttcgccttgc gcgtcggcgc atgcgccggc attgcgcaga tgcggcgaga
     4681 gcagcgcata ggcgtagagc gccgccatca gcgcagctcc gaacaggtat ggcgccgacg
     4741 gcgaccagcg atagagcgca gttgcaatca ttggaccgaa gatgaacccg gcgccgttcg
     4801 tcgcgcccac gagtccggcg actgcgccct gctcgtcggg atcgaccgcg agcgacgcgg
     4861 ctgctgcata acccggccgc accaggccga agccgagacc ggacaggatc agcgcaaaga
     4921 cgagcggccc gaactgatgg ccgatcgcaa agagcagatt cgcggcgagc gcgacgattg
     4981 caccccagcg aatcagcacg cggacggaga tgttgaagcg ctgcacgatg acgagctgtg
     5041 cgaacagtgc cgcaagcgac gacgcgacga gcccgatgcc ggta
//
LOCUS       DASZMC010000418         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5122575,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000418 DASZMC010000000
VERSION     DASZMC010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5122575"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..317)
                     /locus_tag="VG274_08660"
     CDS             complement(108..317)
                     /locus_tag="VG274_08660"
                     /inference="COORDINATES: protein motif:HMM:NF001940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWE06765.1"
                     /translation="MGALSIWHILILAIVALLLFGGRGKISDLMGDVAKGIRSFRKGL
                     NEPDEDRAIPDRRDEPAKKDSTSGQ"
     gene            complement(410..>1749)
                     /locus_tag="VG274_08665"
     CDS             complement(410..>1749)
                     /locus_tag="VG274_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06766.1"
                     /translation="VTRRRLHDEGMRLALDSLSRFRILAEAHHVELRDAVATAAARDA
                     SNGQEFVRRAEAAWGGPIRILSGEEEARLAAQGVLAGIPAADGLVADLGGGSLDMVTL
                     KAGQTGQALTLPFGPLRLIDAAKGEREQARTIVEEGLGAAKQLGNLKGRSLYAVGGVW
                     RSFALVDIDEQNYPLHVLHNYTIPGERAIKLSRVVARLSRKSFERMRIVSRRRAEALP
                     FGAIVLEELLAATGLATVVISAYGLREGLLYAQLSTEERAKDPLIAYAAATNARISRT
                     PQHAHEMFDWLAPLFPDESEEHKRLRHAVCLFSDMGWRRHPDDRALGTFSQVLTAPFA
                     GADHRTRALIATAIFHRYSGDEDFPREFALGGLLDAEDSALARRIGVAARLAFALSAS
                     ATGELANYGLRLTPSRAVLEVPRRRAMIAGEPAQKRLSAIASLLGRKGDILVV"
BASE COUNT          298 a          587 c          581 g          283 t
ORIGIN      
        1 gctcggcacc tcccccacac gtgggggagg aaaaccacca tgggaagttt ggacaaggaa
       61 acgaccggca cggtgcgggc caggggtacg cggagcgtca tcgaaaacta ctgccccgag
      121 gtggaatcct tcttcgcggg ctcgtcgcgc cgatccggga tggcgcgatc ttcgtcgggc
      181 tcattgagtc ccttgcggaa gctgcgaatg cccttggcca cgtcgcccat cagatcggaa
      241 atcttgcccc ggccgccgaa cagcagcagc gccacgatgg cgagaatgag tatatgccag
      301 atgctcagcg cacccatgag actctccttc agcccggggg ctgatcaatg tttccccgct
      361 atgatgcgcg cgggagtgtt taagctccat tcacgcgggc gaagcaactt cagaccacga
      421 ggatatcgcc cttgcggccc agaagcgagg cgatggcgga cagccttttc tgcgccggtt
      481 cgcccgcaat catggcccgg cgccggggca cttcgagcac cgcccggctg ggcgtgagcc
      541 tgagcccgta attggccagt tcccccgtgg ccgaggcgga aagcgcgaag gcgagccgcg
      601 cggccacgcc gatgcggcgc gcgagcgcgc tgtcctccgc atccaggagc ccgcccagcg
      661 cgaactcgcg cgggaaatcc tcgtcacccg aatagcggtg gaagatggcc gtagcgatca
      721 gcgcgcgcgt ccggtgatcg gcgccggcga agggcgcggt gagcacctgg ctgaaagtgc
      781 cgagcgcgcg gtcgtcggga tggcgcctcc agcccatgtc ggagaacagg cacaccgcgt
      841 gacgaaggcg cttgtgctcc tccgattcat cggggaagag cggcgccagc caatcgaaca
      901 tttcgtgggc atgctggggc gtgcggctga tgcgcgcgtt ggtcgccgcg gcataggcga
      961 tcagtggatc cttggcgcgc tcctccgtcg acaattgcgc gtacagcagc ccttcgcgca
     1021 gtccgtaggc cgagatcacg acggtggcga gtcctgttgc ggcgaggagc tcttccagaa
     1081 caatggcgcc gaagggaagc gcttcggcgc gccgccgcga cacgatgcgc attctctcga
     1141 aggacttgcg cgacaggcgc gcgacgactc tggaaagctt gatcgcgcgc tcgcccggga
     1201 tcgtgtaatt gtgcagcacg tggagcgggt agttctgctc atcgatgtcg accagcgcga
     1261 agcttcgcca cactccgccc acggcataga gcgagcggcc ctttaaattg ccgagctgct
     1321 ttgccgcgcc gaggccttcc tcgacaattg tccgcgcctg ttcgcgctcg cctttcgccg
     1381 catcgatcag gcgcagaggg ccgaagggca gcgtgagcgc ctgtcccgtc tggcccgcct
     1441 tcaacgtcac catgtcgagg ctgccgccgc ccagatccgc gacaagtccg tcggcggcag
     1501 gaatgccggc cagcacgcct tgcgctgcga gccttgcttc ctcttcgccc gagaggatgc
     1561 ggatcgggcc gccccatgca gcttccgcgc gccgcacgaa ttcctgtccg ttcgaagcgt
     1621 cgcgggccgc cgccgtcgcc accgcgtcgc gcagctccac gtgatgcgct tcggcgagaa
     1681 tgcgaaaccg cgagagcgaa tcgagcgcga gccgcatgcc ctcgtcgtgc agccgtcgcc
     1741 gcgtgacca
//
LOCUS       DASZMC010000419         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2649613,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000419 DASZMC010000000
VERSION     DASZMC010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2649613"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VG274_08670"
     CDS             <1..615
                     /locus_tag="VG274_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06767.1"
                     /translation="IAERFWSPASVNNIPDMYRRLEIADAELDRIGLTQNTAYRDLLE
                     GLAGSSEIGPVRVLADVTTPGDLGLRHRVNPNYRQDTPLNRFVDTARPDSAVARHFES
                     AVDRYLSNRGDTAARDEIRRSLTLWAANDAQLRPSIEQRAILRDAGPVSAMLSDIANR
                     ALAAMNGATANKSAADLAAAQKPIGEVRLAVATAIAKLIAAVSR"
     gene            612..>2119
                     /locus_tag="VG274_08675"
     CDS             612..>2119
                     /locus_tag="VG274_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06768.1"
                     /translation="MTTASDEKPFTRLELALGAFYAFVIFWINLRICRDFFRNVSAPN
                     NSMHGFWSGMARWADGAWFHPAWWPYWDGGIPFELTYAPGIPFFTSLVASARHVTQLL
                     AFNSVTGALCILAPLTLFLMAWVLTRAPGWAFIAALVYSLSSPTQLLAPDGNFAWRAF
                     WDARRIYLVGVWDDTPHLAAVALLPLIVLFLSLSIRRRRPIYYAAAALCIALASFCSA
                     FGPTDTFLAAVCLVFVLRREHLVSNIAIVAGIGVYAWAMDAPFLSPAVFTAIRESSSR
                     DGTGWSVASWTALAIVIFGWILLWIGLRRWTSDWRLRFFGLFAYLMSAIPMLGEYLNR
                     QFLPQPGRYKLEMELAIALFAVFAIRHTAARLPVSVRVGALLIVLAFAGEQIVSDRAF
                     AKNIVSPADPSRMIEARASLWADRNLPGVRISMPGSIAMWSNLFSPVQQFAGGAWSMA
                     YNQVQQRANDTVQNAEDPVTPLLWLRAYGVGAICVSGPKSPEFWKAVRRPEK"
BASE COUNT          317 a          785 c          577 g          440 t
ORIGIN      
        1 atagccgaac ggttctggtc gcccgcatcg gttaacaaca tcccggatat gtatcggcgt
       61 ctcgagatcg ccgacgcaga gctcgatcgc atcgggctca cccaaaacac cgcctaccgc
      121 gacctgctcg aaggactcgc cggatcatcc gagatcggcc ccgtccgtgt gctcgcggac
      181 gtgaccactc ccggcgacct cggccttcgc catcgcgtca atcccaatta ccggcaggac
      241 acgccgctca atcgtttcgt cgataccgcg cgcccggaca gcgctgtcgc ccgccatttc
      301 gagtccgccg tggatcggta cctctcgaac cgaggcgata ccgccgcgcg cgatgagatc
      361 cggcgctcgc tcaccctttg ggccgccaac gatgcgcagt tgcgcccgtc gatcgagcaa
      421 cgcgctatcc tgcgcgacgc cggtcctgtt tccgccatgc tctcggatat tgcgaaccgc
      481 gcgctcgccg caatgaacgg tgcgaccgct aacaagagcg ccgccgacct ggccgccgcc
      541 caaaagccca tcggcgaagt gcggctcgcc gtggcgactg ccatcgcgaa actcattgcc
      601 gcggtatcca ggtgacaacc gcgtccgacg agaaaccgtt tacgaggctc gaactcgcgc
      661 tcggggcctt ttacgccttt gtcatcttct ggatcaacct ccgcatctgc cgcgattttt
      721 ttcgcaatgt gagcgcgccc aataactcca tgcacggctt ttggtccggc atggcgcgct
      781 gggccgatgg cgcctggttc caccccgcct ggtggcccta ctgggatggc ggcatcccct
      841 tcgagctcac ctatgcgccc ggcattccct tcttcacctc gcttgtcgcc tccgcgcgcc
      901 acgttacaca actgctcgcc ttcaactccg taaccggcgc tctctgcatc ctcgcgccgc
      961 tcactctctt ccttatggcc tgggttctca cgcgtgcgcc cggctgggct tttatcgcgg
     1021 ccctcgtgta ttcactcagt tcgcccaccc agttgctcgc gcccgatggc aatttcgcct
     1081 ggcgcgcctt ctgggacgcg cgccgtatct acctcgtcgg cgtctgggac gacaccccgc
     1141 acctcgccgc cgtcgctctg ctgccgttga tcgtcctgtt cctgtccctt tccattcgac
     1201 gccgccgccc tatctattac gccgccgccg ccctctgcat cgctcttgca tcgttctgca
     1261 gcgccttcgg ccccaccgac acctttctcg ccgccgtatg ccttgtcttc gttctgcgcc
     1321 gcgaacacct cgtttccaac attgccatcg tggcgggcat cggcgtctac gcctgggcta
     1381 tggacgcgcc gttcctctct cccgccgtct tcaccgcgat tcgcgaatcc tccagccgcg
     1441 acggtacggg atggagcgtt gcctcatgga ccgcgctcgc catcgtgatc ttcggctgga
     1501 tactcctctg gatcgggctc cgccgctgga cctcggattg gcgcctgcgt ttcttcggcc
     1561 tcttcgcgta tctgatgtcc gccatcccga tgctgggcga atatctaaac cgccaattcc
     1621 tgccgcaacc cggccgctac aagctcgaaa tggaactcgc catcgcgctg ttcgcggtct
     1681 tcgccattcg ccacacagcc gcgcgtctgc cggtttctgt cagagtcggg gctctcctca
     1741 tcgtgctcgc cttcgccggc gaacagatcg tgagcgaccg cgccttcgcc aaaaacatcg
     1801 tgtccccggc cgatccctcg cggatgatcg aagcgcgtgc atccctctgg gccgatcgga
     1861 atctccctgg cgtgcgcatt tccatgcccg gctccattgc catgtggagc aatctgttca
     1921 gccccgtcca gcagttcgcg ggcggagcgt ggagcatggc ctacaaccag gtgcagcagc
     1981 gcgccaacga cacggtccaa aacgcggaag atccggtgac gccactgctc tggctgcggg
     2041 cctatggggt cggcgcgatc tgcgtatccg gccccaaaag ccccgagttc tggaaagcgg
     2101 tcagacggcc ggagaagtt
//
LOCUS       DASZMC010000420         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_469924,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000420 DASZMC010000000
VERSION     DASZMC010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469924"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..581)
                     /gene="rsmD"
                     /locus_tag="VG274_08680"
     CDS             complement(9..581)
                     /gene="rsmD"
                     /locus_tag="VG274_08680"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWE06769.1"
                     /translation="MRITGGRFGGRVLAAPADARVRPTSDKVRQAVFNVLAHNDFGLG
                     FSLEGARVVDLFAGTGAMGLEALSRGAGFCLFIDDSAESRALLRQNVETLALTGATKI
                     WRRGATMLGAMSAVAGGPFDLALLDPPYRMNLIAPALASLRDGKWLAPDALVVAECAR
                     DEFLNEQMGFSRLDSRIYGETEVVFLGFKM"
     gene            complement(572..1000)
                     /locus_tag="VG274_08685"
     CDS             complement(572..1000)
                     /locus_tag="VG274_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE06770.1"
                     /translation="MSEALRIAVEASDAEIENTIRTRLGAFNRTAVDKPERQHFNVVL
                     RDCEGCMRGGLIASVNFDVMLIEDLFIEEAHRRGGHGAKLVALAEEEGRLRGARRACV
                     STFSWQARPFYEKLGFRVFGELPYCGGAHVLYWLDKSLCA"
     gene            1230..1913
                     /locus_tag="VG274_08690"
     CDS             1230..1913
                     /locus_tag="VG274_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWE06771.1"
                     /translation="MGNGFAEGFGSLFLLIFAAAFPIVNPAGSALIFLDMTRHAAHRT
                     REGLARLVAFYSFLVLNVSLFIGSYVLEFFGISVPALRVAGGIVVAAAGWKFLQEHEA
                     EGVEEAARGDAALADNYAGMAFYPFTMPITTGPGTISVMIALGTSHTSHDPLQHRIEF
                     GLAALLANAVLALLVYLCFAHADRVRRLLGNTGTTIALRLAAFILFCIGVQILWSGVA
                     EMLAPWKLH"
     gene            2005..>2660
                     /locus_tag="VG274_08695"
     CDS             2005..>2660
                     /locus_tag="VG274_08695"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HWE06772.1"
                     /translation="MRWPRFVLPAMLVLAACGAAAPACAQIDSAPKVHARLIAEQSEI
                     APGRRVTIALEENIRPGWHTYWRNPGDAGEPTQLQWTLPAGWRASPIAWPYPKDLPVG
                     PLMDYGYEGRPWLLLDIAAPDDAKAGTRVVLRAQASWLVCKEVCIPEDATLSLPLTIS
                     TSPPAPDPSVAAQFAAARDRLPVVSPWPARFHLGASLDLFLAAPKLAAASVTDARFFP
                     "
BASE COUNT          438 a          864 c          837 g          521 t
ORIGIN      
        1 aagcaaaatc acattttgaa accgagaaac accacttccg tttcgccata gattcgcgaa
       61 tcaaggcgtg agaaacccat ctgctcgttc aagaattcgt cccgcgcgca ttcggcgacg
      121 acgagcgcat ccggcgcgag ccattttccg tccctcagcg aggcgagcgc gggcgcgatg
      181 agattcattc gatagggcgg gtcgagaagc gccaggtcga acgggccgcc cgcgacggca
      241 ctcatcgctc caagcatggt cgcgccgcgg cgccaaatct tggtcgcgcc ggtgagcgcg
      301 agggtttcca cgttctgccg gaggagcgcc cggctctcgg cggaatcgtc gatgaacagg
      361 cagaagcctg cgccgcgcga cagagcttcg agtcccattg cgccggtgcc cgcgaacagg
      421 tccaccacgc gcgcgccttc gagagaaaat cccaagccga aatcattgtg ggcgagaacg
      481 ttgaacaccg cttgccgtac cttgtccgaa gtcggccgca cgcgagcgtc cgcaggcgcg
      541 gcgagtacgc ggccgccgaa acgcccgccc gttatgcgca cagggatttg tccagccagt
      601 acagcacgtg ggcgcccccg cagtatggca gctcgccgaa tacgcgaaag cccagcttct
      661 cgtagaaggg ccgcgcctgc cagctgaagg tggatacgca ggcccggcgc gcgccgcgca
      721 atcggccttc ttcttcggcc agcgccacga gcttcgcacc atggccgccg cggcggtgcg
      781 cctcttcgat gaagagatcc tcgatcagca tgacatcgaa attcaccgat gcgatcagac
      841 cgcctctcat gcagccttcg caatcgcgca gtacgacatt gaagtgctgc cgttcgggtt
      901 tgtccacggc ggtcctgttg aacgccccga gcctcgtgcg gatcgtattt tcgatctccg
      961 cgtcgctggc ctcgaccgct attcttaaag cttcgctcac atgcgtccct cgaaccgttt
     1021 gcaaccttat tttgtcatgg cccggctcga ccgggccacc cagccagcgc gcgtccgcgc
     1081 gatgaattga gggtcgcgct gggtcatctg ggtggcccgg tcgagccggg ccatgacaaa
     1141 tttttagtca agccgctcag aaaccgcttc accttcgcgc gggctgttcg aagcatgata
     1201 cacaacgcgc agtaggaggg acgcctagca tgggcaatgg gtttgcggag ggtttcggaa
     1261 gcctgttcct cctcattttt gcggcagcgt tcccgatcgt caatcccgcg ggcagcgcgc
     1321 tgatctttct ggacatgacg cgccacgccg cgcaccgcac gcgcgaggga ctggcgcgtc
     1381 tcgtcgcctt ctacagtttt cttgttctga acgtctcgct gttcatcggc agttatgtgc
     1441 tcgagttctt tggcatctcc gtgccggcgt tgcgcgtggc cggagggatt gtggtcgccg
     1501 ccgcaggctg gaaattcctg caggagcatg aggcggaggg cgtcgaagag gccgcgcgcg
     1561 gcgacgcggc tttggccgat aattatgcgg gcatggcgtt ctatcccttc accatgccga
     1621 tcacgacggg gccgggaacg atctcggtga tgattgcgct tggcaccagc cacacgtctc
     1681 acgatccttt gcagcatcgc atcgagtttg gcctcgcggc gcttctggcc aatgccgtgc
     1741 tcgcgctcct cgtctatctg tgcttcgccc atgcggaccg ggttcgcagg ctgctcggca
     1801 atacggggac gacgatcgcg ctccggctgg cggctttcat tctcttctgc attggggtgc
     1861 agattctgtg gagcggagtt gcggaaatgc tggcgccctg gaaattgcac tgaagcgtct
     1921 cttgtaccgg gaacagcgat cttaacagcg atattgcagc gtgcaggctg gcggcccatg
     1981 gccccgcccg ctatagcggc ggtcatgcga tggccccgat ttgtccttcc ggcgatgctg
     2041 gtcctggccg cgtgcggcgc ggctgcgccg gcatgcgccc agatcgattc ggcgccgaag
     2101 gttcacgcgc gcctgatcgc cgagcagagc gagatcgcgc cggggcgccg cgtcaccatt
     2161 gcgctcgaag agaatattcg ccccggctgg cacacctatt ggcgcaatcc cggcgatgcg
     2221 ggcgaaccca cgcaactcca gtggacattg ccggcgggct ggcgcgcgag cccgatcgcc
     2281 tggccttatc cgaaagatct gccggtcgga cccttgatgg attacggcta tgaagggcgg
     2341 ccgtggcttc tgctcgatat cgctgcgcct gacgatgcga aggcgggcac gcgcgtcgtg
     2401 ctgcgtgcgc aggcgagctg gcttgtctgc aaggaagtgt gcattcccga agatgccacg
     2461 ctcagcctgc cgctcacgat cagcacatcg ccaccggcgc cggatccgtc tgtcgccgcg
     2521 cagtttgccg ccgcacgcga ccgtcttccg gtcgtctcgc cctggcccgc gcgcttccat
     2581 ctcggtgcgt cgctcgatct ttttctggcg gcgccgaagc tcgcggcggc gagcgtcacg
     2641 gatgcgcggt tctttccctt
//
LOCUS       DASZMC010000421         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4313385,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000421 DASZMC010000000
VERSION     DASZMC010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4313385"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VG274_08700"
     CDS             complement(<1..384)
                     /locus_tag="VG274_08700"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06773.1"
                     /translation="MRIARTLQEADAFGPTAVAIGNFDGLHIAHRELLSAVSRAAREH
                     ALAPSVLMFDPHPATIVAPHRVPKLLSTQGERCSLMAREGIEHVLILPFTIAVSRWTP
                     REFAERVLAKAFRAKAVIVGENFRFG"
     gene            complement(387..1184)
                     /locus_tag="VG274_08705"
     CDS             complement(387..1184)
                     /locus_tag="VG274_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWE06774.1"
                     /translation="MPDSTLRITVLGSGTSSGVPTIGCKCAVCASTDPRDTRLRPSIL
                     LQYDGRNVLIDATPDFRQQVLRARIDRIDAILLTHAHADHILGLDDMRPLNYHQGGSI
                     PVYGTEQTFEVVRRVFQYAFDSKPSQSSSPRLDLRPITCEPFELFGRQIVPLPLPHGN
                     LPILGFRTGRMAYLTDHSDIPEDLKPQLRHLDVLFLDALRYRPHPTHSTVDRAVQWVK
                     ELEPRQAFFTHMSHDVSHAQAELTLPPNARLAYDGLQIEVNDGEAQG"
     gene            complement(1191..1505)
                     /locus_tag="VG274_08710"
     CDS             complement(1191..1505)
                     /locus_tag="VG274_08710"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HWE06775.1"
                     /translation="MSDPDYPLPPPTFEFLVFSLKTQAEVHLGLVSFGEKTEPPNLEA
                     ASHAIDMLGMIVDKTHGNLSLEEQRLIENSLTELRFRFVQVRAESEKSAPQPSQPEGA
                     AS"
     gene            1607..2047
                     /locus_tag="VG274_08715"
     CDS             1607..2047
                     /locus_tag="VG274_08715"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HWE06776.1"
                     /translation="MPRHFRLEDAESLLPEVEQALRDAIFQKSEYEAADGELDRAMQR
                     IRMAGGSRVSPGPILALRARKDSAASALAAVMERINNWGVQVKDLDRGLIDFPTLYKG
                     REVLLCWKLGEERIGYWHGLEEGYRGRKAIDDEFRAEHRGAAPN"
     gene            2147..>2365
                     /locus_tag="VG274_08720"
     CDS             2147..>2365
                     /locus_tag="VG274_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HWE06777.1"
                     /translation="MAKPTQEDVAQYSDMLAAMGAEPRLRIMRLLLSAHPEGLVVGNI
                     GEELDIAPSTLSHHLDKLKNEGLVSVRRE"
BASE COUNT          481 a          638 c          808 g          438 t
ORIGIN      
        1 gccgaagcga aaattttcgc cgacgatcac ggccttggca cgaaaggctt tggccaatac
       61 acgctcggcg aattcgcgcg gcgtccagcg tgagaccgcg atggtgaagg gcaggatgag
      121 gacgtgttcg atgccttcgc gtgccatgag gctgcaacgc tcgccttgcg tggagagcaa
      181 cttgggcacg cggtgcggcg cgacgatggt ggcgggatga ggatcgaaca tcagcacgga
      241 gggcgccaga gcgtgttcgc gcgcggcgcg cgaaacggcg gacaacaatt cgcggtgcgc
      301 gatatggagg ccgtcgaaat tgccgatggc tacggccgtg ggcccgaacg cgtcggcttc
      361 ctgcagagta cgcgcgattc gcatcgctag ccctgcgcct cgccgtcgtt gacctcaatc
      421 tgcaaaccgt cgtaggccag acgcgcattg ggaggcagcg tcaactccgc ttgcgcgtgg
      481 cttacgtcgt gcgacatgtg agtgaaaaat gcctggcgcg gttccagctc cttcacccat
      541 tgcacggcgc gatcgacggt ggagtgcgtg ggatgcgggc ggtaacgcaa agcatctaag
      601 aacaggacat ccagatgccg aagctgcggc tttaaatcct cgggaatatc gctgtggtcg
      661 gtgagatacg ccatgcggcc ggtgcggaat ccgagtatcg gcaaattgcc gtgcggcaaa
      721 ggcagcggaa cgatctggcg gccgaagagc tcgaacggct cgcaggtgat gggccttaga
      781 tcaagccgcg gcgacgaaga ttgcgagggc ttcgaatcaa acgcgtattg aaatacgcgg
      841 cgcaccactt cgaatgtttg ttccgttccg taaacgggaa tggagccgcc ctgatggtag
      901 ttcaacgggc gcatatcgtc caggccgagg atgtgatcgg catgcgcgtg cgtcagcagg
      961 atggcatcga tgcgatcgat gcgcgcgcgc agcacctgct ggcggaaatc gggcgtggcg
     1021 tcgatcagca cgttgcggcc gtcgtactgt agcaggatgg aggggcgcag gcgcgtgtcg
     1081 cgcggatcgg tggacgcgca cacggcgcac ttgcagccga tggtgggcac gccagagctg
     1141 gtgcccgatc caaggacggt gatccgcagt gtgctatccg gcatgaacct ttaggacgcc
     1201 gctccctcgg gctgggaagg ctgaggcgcg gacttttcgg attccgcgcg aacctggacg
     1261 aatcggaagc gcagttcggt gagcgagttt tcgatcaggc gctgttcttc gagcgagaga
     1321 ttgccatgtg ttttatcgac aatcatgccg agcatgtcga tggcgtgcga ggcagcctcc
     1381 agattgggcg gctccgtctt ttcgccgaag ctcaccagac cgaggtgcac ttctgcctgg
     1441 gttttcagcg agaacaccag aaactcgaag gtggggggag gaagagggta gtcgggatcg
     1501 ctcatcggtt taatctcgcc gggcctgttc tatgagccgc gggaacgcgc gccgttggcg
     1561 tctgcccggc agctggcccg tctcttctag tgtagtgtgg aatggaatgc cccgacattt
     1621 caggctggaa gacgcggaat cgctgctgcc ggaagtggaa caggcgctgc gtgacgccat
     1681 cttccagaaa tcggagtacg aggccgcgga cggcgaactg gatcgcgcga tgcagcggat
     1741 tcgcatggcc ggaggatcgc gcgtgagccc gggtccgata ttggcgctgc gggcgcggaa
     1801 agattccgcg gcctcggctc tggccgccgt gatggagcgc atcaacaact ggggcgtgca
     1861 ggtgaaggac ctggacaggg ggctgatcga tttccccacg ctatataaag gtcgcgaagt
     1921 gttgctgtgc tggaaactgg gtgaggagcg catcggctac tggcatggct tggaagaggg
     1981 ctatcgcgga cgcaaagcca tcgacgacga gtttcgggcg gagcatcgcg gcgccgcgcc
     2041 gaattagcgc aacgcctaag acaccgcacc aaacagattt cacatgtcac ggctcttgtt
     2101 atttttctat aattctataa tgatagaact atagaaggat aactcagtgg ccaaaccaac
     2161 ccaggaagac gtggctcagt actcggatat gctcgcagcg atgggagccg agccgaggct
     2221 gcggatcatg cggctgcttc tgtcggccca tccggaagga ctcgtggtgg gcaacatcgg
     2281 cgaagagctc gacatcgcgc cgtcgacact ttcccatcat ctggacaaac taaagaacga
     2341 aggcctggtg agtgtgcgcc gggaa
//
LOCUS       DASZMC010000422         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4584090,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000422 DASZMC010000000
VERSION     DASZMC010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4584090"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1419
                     /gene="murA"
                     /locus_tag="VG274_08725"
     CDS             160..1419
                     /gene="murA"
                     /locus_tag="VG274_08725"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWE06778.1"
                     /translation="MDRIRIHGGARLNGEIAISGAKNAALPLMAACLLSDEPLVLRNV
                     PRLADIDLMEKLLQSFGVAVHHRSGAVALSADAIRNTTAVYDLVRKMRASFLVLGPLV
                     ARRGEARVSLPGGCAIGARPVDLHLRALLALGAAIELREGYVVATAPAGLRGARIEFP
                     AVSVGATEHALMAAALAKGETLLVNAAREPEIEDLGRCLQAMGARISGLGSPCITVEG
                     VDELHGAEHRVLPDRIETGTYALAAAIAGGDVDLVDTDCDLIGAVVPLLEAVGAEIVP
                     NDRGMKVRRNGARPIAVDVATAPFPAFPTDLQAQFMALMTTAEGVSRIRETIFENRFM
                     HVPELSRMGADIRIEGDTAIVTGVERLKGAPVMATDLRASVSLVLAGLAAEGETIVSR
                     VYHLDRGFERIEEKLTGVGARVERISE"
     gene            1416..1859
                     /locus_tag="VG274_08730"
     CDS             1416..1859
                     /locus_tag="VG274_08730"
                     /inference="COORDINATES: protein motif:HMM:NF022612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HWE06779.1"
                     /translation="MTAAKVTLAAADAEDLEAISARLQDAVAQVKDLVFLPKSRRFAG
                     LFNRFKWESAGKHGGARVRTGLHIDGVLSAKATHLKRGNPDAVASLLAVRFLPRSGDD
                     PGGTIELIFAGGGALRLEVECIAAELTDLSGEWLARGRPVHEAES"
     gene            2173..2652
                     /locus_tag="VG274_08735"
     CDS             2173..2652
                     /locus_tag="VG274_08735"
                     /inference="COORDINATES: protein motif:HMM:NF002769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HWE06780.1"
                     /translation="MTDASRFRLASVELDERTLVRRTREIEQEREIAIYDLLEANSFV
                     PCGSSGGPYRLVLAVQENRLVFNIRLESGAPHGRVMLSLTPFRRVIKDYFLVCESYFK
                     AIRNAPPAQIEALDMGRRGLHDEGSELLRERLKGKIEVDSDTARRLFTLLCVLHLKG"
     gene            2657..>2868
                     /locus_tag="VG274_08740"
     CDS             2657..>2868
                     /locus_tag="VG274_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06781.1"
                     /translation="MRGPNGEELPGAVLFACTLNAVRSPIAAALMRHLYGRFVYIDSV
                     GARAGELDPFAMEVMEEIGIAIGKHRP"
BASE COUNT          482 a          953 c          915 g          518 t
ORIGIN      
        1 aaaccccgcg ggcgatcggc cgcccaaaaa gcctgtttga ccggtgtgcc tggcggccgc
       61 ttgattccgg tgcgaagccc gctatctgaa cgcaatcccg cgcgctaaaa ggcccgaccg
      121 gccgcgcctg tttttcccgg ccttgtcccg agaggggtca tggatcgaat ccgaattcac
      181 ggcggcgcca ggctgaacgg cgaaatcgcc atcagcggcg ccaagaacgc cgcgctcccc
      241 ctcatggcgg cctgcctgtt gagcgacgag ccgctcgtgc tccgcaacgt cccccgcctt
      301 gccgacatcg atctgatgga aaagctcctc cagagcttcg gcgttgccgt tcatcaccgc
      361 agcggcgctg tcgcgctgtc ggccgatgcc atccgcaaca ccaccgccgt ctacgatctg
      421 gtgcgcaaga tgcgcgcgag ctttctcgta ttggggccgc tggtcgcccg ccggggcgaa
      481 gccagggtct cgcttcccgg cggctgtgcg atcggcgcac ggcccgtcga tcttcatctg
      541 cgcgcgctgc tggccctcgg cgctgcgatc gaactccgcg agggttatgt cgtggcgacc
      601 gcgcctgcgg gcctcagagg cgcgcgcatc gagtttccag ccgtctcggt cggcgccacc
      661 gaacacgcgc tgatggcggc cgccctcgcc aagggcgaaa cgctgctcgt caatgccgcg
      721 cgtgagccag aaatcgagga tctcgggcgc tgtcttcagg ccatgggcgc gcgcatctcg
      781 ggacttggaa gcccgtgcat caccgtcgaa ggcgtcgacg aattgcatgg cgccgaacac
      841 cgcgtgctcc ccgatcgcat cgagaccggc acctatgcgc tggcggcggc gatcgcgggc
      901 ggcgatgtcg atcttgtcga caccgattgt gatctgatcg gtgcggtggt gccgcttctc
      961 gaagccgtag gcgccgagat agtgcctaac gatcgcggca tgaaagtaag gcgcaatgga
     1021 gctcgcccga tcgcggtcga tgtcgcaacg gcgccgttcc cggcctttcc caccgacctg
     1081 caggcgcagt tcatggcgtt gatgacgacg gccgagggcg tttcccgcat tcgcgagacc
     1141 atcttcgaga accggttcat gcatgttccc gaactatcgc gcatgggcgc ggacatccgc
     1201 atcgagggcg acaccgcgat tgtcaccggc gtggagcgcc tgaaaggcgc tccggtcatg
     1261 gcgaccgacc tgcgggcaag cgtcagcctt gtcctcgcgg ggctcgccgc ggaaggcgaa
     1321 accatcgtca gccgcgtcta tcatctcgat cgcggtttcg agcgaatcga ggaaaaactg
     1381 accggcgtcg gcgcgcgcgt cgagcggatc tccgaatgac cgccgccaaa gtcacgcttg
     1441 cggcagccga cgcggaggat ctcgaggcta tttccgcgcg cctccaggat gccgtcgcgc
     1501 aagtgaagga tctcgtgttt ctgccgaaat cgcggcgctt tgccggcctc ttcaatcgct
     1561 tcaaatggga gagcgcggga aaacatggcg gcgcccgcgt gcggaccggg ctccacatcg
     1621 acggcgtgct gtcggccaag gccacgcacc tcaagcgcgg caatcccgac gcggtggcgt
     1681 cgctgcttgc ggtccgattc ttgcccagat ccggggatga tcccggcggc acgatcgagc
     1741 tgatcttcgc gggcgggggc gcattgcggc tcgaagtgga gtgcatcgcg gccgaactca
     1801 ccgatctctc cggcgaatgg ctggcgcgcg gccgccccgt gcacgaagcc gaatcctgag
     1861 cgtgatccgt ttcgatttga tgggatggcg ctctcatgga ccgaatccaa cacttcacac
     1921 cgaaaaattg tcatggcccg ccaccccggt ccgcgcggga gcgcggccgg agtgtaaact
     1981 cgagcgggcc acccagacga aaccgctcag agcgttcatt cagggcgcgg acgcgcgctg
     2041 gctgggcggc ccggctaaac cgggccatga catttttttg gtttcgaatc tcagaaccgg
     2101 accactttcc cgcaattcat cctcactgtc ttccacccgg tttaaccggg tggctcattg
     2161 tgaatctctc ttatgaccga cgcctcgcga ttccgtctcg cttcggtcga actcgacgag
     2221 cgcacgctgg tgcggcggac acgcgagatc gagcaggaac gggagatcgc gatctacgat
     2281 cttctggaag ccaacagctt cgttccgtgc ggctcgtcgg gcggtcccta ccggctggtg
     2341 ctcgcggtcc aggaaaaccg cctcgtcttc aacatcaggc tcgaaagcgg cgccccccat
     2401 ggccgggtca tgctttcgct cacgccgttt cggcgcgtca tcaaagatta cttcctggtc
     2461 tgcgaaagct atttcaaggc gattcgcaac gcccccccgg cgcagatcga ggcgctcgac
     2521 atgggtcggc gcggcctgca cgatgaaggc tccgaacttc tgcgcgagcg tctgaagggc
     2581 aagatcgagg tcgattccga tacggcgcgg cggctgttca ccctgctctg cgtgctgcac
     2641 ctgaaaggct gacgcgatgc gcgggcccaa tggcgaggaa ttgccgggcg ccgttctctt
     2701 cgcatgcacg ctcaacgccg tgcgcagccc gatcgccgca gccctgatgc gccatctcta
     2761 cgggcgtttc gtttacatcg actcggtcgg cgcgcgggca ggtgagctcg atccctttgc
     2821 gatggaagtc atggaggaga tcggcatcgc catcggcaag cacaggcc
//
LOCUS       DASZMC010000423         4468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4584410,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000423 DASZMC010000000
VERSION     DASZMC010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4584410"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VG274_08745"
     CDS             <1..906
                     /locus_tag="VG274_08745"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HWE06782.1"
                     /translation="GKAGMESQCLGLAEALGLDPTIKRIGLHEPWRRFAPHLRLGLRH
                     AFTGDPLVPPWPHLVIATGRISVPGSLYVRRMNPRTRNVQIQNPGISPRHFDLVVTPI
                     HDLLGGANVIHTMGALHRVTGERLAREAALLASRIGEQPRPRIGVLIGGGNAAYRFAA
                     DDAHALAAELARHAAASGASLLVTTSRRTGTENLEILRTALADTRAFLWDGTGDNPYY
                     GILGLADVLVVTADSVNMITEACATGRAVYIYGLPGGSEKFARFHEALTLRGYAQFYQ
                     GSLESFVTDRLDEMPRIVRAVRQIA"
     gene            complement(1169..2422)
                     /locus_tag="VG274_08750"
     CDS             complement(1169..2422)
                     /locus_tag="VG274_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWE06783.1"
                     /translation="MWRLLSALAAFSITLQSPPARADASSLLSSWVQMAPGGWSEARA
                     VVHGAKCPDAVIDRANVAMRLRAPPAPEFANLLCTVTLPKGARIASILGHALPLPDVA
                     PHRIMVIGDTGCRIKGWTVQACNDPAKWPFPHIARVAAKLKPDLVIHVGDYLYRESAC
                     PSGNRACAGSPSGDNWASWRADFFAPAAPLLDAAPLVFVRGNHEECARSGKGWLRLLG
                     PLPDDPKAPCTDHVAPWGVAMGGLTLAVMDDAHAPDPVAPGELVTLYRADLAAVAKIA
                     PAPVWLAMHRPIWGVVELGFGMVVGGNRTMMAAQSEGGIPDNVSLLLSGHIHTFEAIN
                     YQKDAPPQIVAGEGGDLLDQAPRDLSGRTVGALKISSGLSLPGYGFLLLTRDADRWSV
                     EVMTADGAREALCVFANRHLDCHAK"
     gene            complement(2422..3099)
                     /locus_tag="VG274_08755"
     CDS             complement(2422..3099)
                     /locus_tag="VG274_08755"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWE06784.1"
                     /translation="MHDFVHTCIAFIRLHGAWAGPVIFVVSFGESFVGLNLICPGTTI
                     MVIAGTLVRWQLNPHGVLDLWPVLIGGMLGGIAGDAISFWFGRRFGHLLEKHWFFVRH
                     PEFLPRGYAFFDRFGTASVFIGRFFGPVRACIPLVAGIMVMSWRNFWIANIGSAIVWA
                     PALLLLGTSFRLLTRGLGLVHGEGLAESIAAVVFLMILVWAARRYRALEKLRHAWRRR
                     MGKMAQR"
     gene            complement(3391..>4468)
                     /locus_tag="VG274_08760"
     CDS             complement(3391..>4468)
                     /locus_tag="VG274_08760"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HWE06785.1"
                     /translation="IVPSHVLKQLIRARREVLAAEEIEPSQIQPASVDLRLGPMAWRV
                     RASFLPGPQATVRDKLATVALHEIDLTDGAVLETGCVYVVPLLESLELSFRVSGVANP
                     KSSTGRLDVFTRLIVDRAQSFDRVEPGYRGPLYAEISPRTFPILVRKGSRLNQLRIRR
                     GSPQFTDTQLKRLHADTPLVDGPADIENGLALSVDLKGDPALQQVGWRAKRHTGIIDI
                     DRRDALEAADFWDSIQASRSGTIVLDPDEFYILASREAVSIPPEFAAEMLPFNPLVGE
                     FRVHYAGFFDPGFGHEAGLGRGARAVLEVRSREVPFILEHGQIIGRLVFERLTEPPPE
                     VYGQGIGSNYQRQGLRLSKHFKHG"
BASE COUNT          880 a         1454 c         1396 g          738 t
ORIGIN      
        1 ggcaaggccg gcatggaaag ccagtgtttg ggccttgcgg aagcgctcgg cctcgatccc
       61 acaatcaaac gcatcggact gcacgaaccc tggcggcgat ttgcgccaca tctgcggctg
      121 ggcttgcgcc acgccttcac cggagatccg ctcgtgccgc cctggccgca tctggtgatc
      181 gcgacgggcc ggatcagcgt tccaggctcc ctttatgtgc gccgcatgaa tccccgcacg
      241 cgcaacgtcc agattcagaa tccgggcatt tcgccgcggc acttcgatct cgtcgtcacg
      301 ccgatacatg atcttctcgg cggagcgaat gtgatccaca cgatgggcgc actccaccgc
      361 gttacggggg agcggctggc gcgcgaagcc gcgctgctgg cgtcgcgcat tggcgaacag
      421 ccgcggcctc gtatcggcgt tctgatcggc gggggcaacg ccgcctacag attcgcagcc
      481 gatgatgcgc acgcgctcgc cgccgaactc gcacgccatg cggccgcgtc gggagcgagc
      541 ctcctcgtca cgacgtcccg ccgcacggga acggaaaatc tcgaaatcct gcggaccgct
      601 ctcgccgata cgcgcgcgtt tctgtgggac ggcacaggcg acaatccgta ttacggcatt
      661 ctgggtcttg ccgatgttct tgtcgtcacc gcggactcgg tgaacatgat cacggaagcc
      721 tgcgcgacgg gccgggccgt ctatatctac gggctgcccg gcggttctga gaaattcgcg
      781 cgcttccacg aggcgctcac gctgcgcggc tatgcgcagt tctatcaggg atcgctcgaa
      841 agtttcgtga ccgatcgtct cgacgagatg ccccgcatcg tccgcgccgt ccgccaaatc
      901 gcatagtcaa acgatcagca aggcggcaat tcatccaaaa aaaaaagcac gcggagtcgc
      961 ggaggacacg gagaaaagga tcacagggag acagtgagac aatgagataa attgatccgc
     1021 gcgaacgcgc acaattcttc ctcactgcct cactgtctca ttgtgaatct tgcttttctt
     1081 cgtatcctcc gcggctccgc gtgagatttt gttccttcgg ctactcgggg tccaggcaca
     1141 aattgaccat taggctttcg caatgccgtt atttcgcatg gcaatcgaga tggcggttgg
     1201 caaagacgca cagtgcctcg cgtgcgccat cggccgtcat cacctcgacg gaccatcgat
     1261 cggcgtcgcg cgtcagaagg agaaatccgt aacccggcaa cgacagaccc gatgaaatct
     1321 tcagcgcgcc cacggtgcgg cccgaaagat cgcgcggcgc ctgatccagc aggtcgccgc
     1381 cctcgccggc gacgatctgc ggcggcgcat ctttctggta gttgatcgcc tcgaacgtgt
     1441 ggatgtgccc ggagagcaga agagaaacat tatcgggaat gccgccttcg ctttgcgccg
     1501 ccatcattgt gcgattgccg ccgaccacca tgccgaagcc aagctcgacg acgccccaga
     1561 tcgggcggtg catggcaagc cagaccggtg ccggagctat cttggccacg gccgcaagat
     1621 cggccctgta aagcgtgacg agttcgccgg gtgcaacggg atccggcgca tgtgcgtcgt
     1681 ccatgactgc aagtgtcagc cctcccattg caacgcccca gggcgcgaca tggtcggtgc
     1741 agggcgcctt gggatcgtcg ggaaggggac cgagcaacct cagccatccc ttgcctgaac
     1801 gcgcgcattc ctcgtggttc ccccgcacga acaccaaggg cgccgcgtcg agaaggggag
     1861 cagccggcgc gaagaaatcc gcccgccagc ttgcccagtt gtcgcccgac ggactgcccg
     1921 cgcaggcgcg attgcccgat gggcaggcgg attcgcggta gagataatct ccgacatgga
     1981 tgacgaggtc gggcttcagt ttcgcggcca ctcgcgcgat gtgtggaaag ggccatttcg
     2041 ccgggtcgtt gcaggcctgc acggtccagc ccttgatgcg gcaacccgtg tcgccgatca
     2101 ccatgatccg gtgcggcgca acgtccggca gaggcaaagc gtgccccaaa atcgaggcga
     2161 tccgggcgcc ctttggaagc gtcacggtgc acagaagatt tgcaaattcc ggggctggcg
     2221 gagcgcgaag ccgcatcgcg acgttggcgc ggtctatcac ggcgtccgga catttggcgc
     2281 cgtgcacgac agcgcgtgcc tcggaccatc cgccgggagc catctggacc cagctggaca
     2341 aaagcgatga tgcatcggcg cgtgccggag gggactgcag cgtgatcgag aaagcggcaa
     2401 gagcggaaag aagccgccac atcaacgttg cgccattttg cccatgcgcc ttcgccacgc
     2461 atgacgaagc ttttcgagcg cgcggtagcg ccgcgccgcc caaacaagaa tcatgagaaa
     2521 tacgaccgcg gcgatgctct cggccaaccc ttccccgtgc acaagaccca gacctctcgt
     2581 caacagccgg aaactcgtgc ccagaagcag gagagccgga gcccagacga tcgccgagcc
     2641 gatattggcg atccagaagt tccgccatga cattaccatg atgcccgcga cgagggggat
     2701 gcaggcccgc accggcccaa agaaacggcc gatgaacaca ctggctgttc cgaagcggtc
     2761 gaagaaagca tagccgcgcg gaagaaactc ggggtgcctc acgaaaaacc aatgcttctc
     2821 gaggagatgg ccgaaccttc gcccgaacca gaacgagatc gcatctcccg caattccgcc
     2881 cagcatcccg ccgatcagaa ccggccacag gtcgagaacg ccatggggat tgagctgcca
     2941 gcgcaccagc gtcccggcga tcaccatgat ggtggtcccc ggacagatga ggttcagtcc
     3001 cacgaacgat tcgccgaacg agacgacgaa gatgaccggc cccgcccacg cgccgtgcag
     3061 tctgatgaag gcaatgcagg tatggacgaa gtcgtgcatg cggccgggct tagcacgcgg
     3121 cgcggcggcc atgccaattg ctgtggtccg aaacggtgtc cagaaatgtc atgaggcggc
     3181 cggcgctgta cgcgccgtag ccgcctcatg ctgcccccgt caatgcggac cctcgtcgct
     3241 gccgcctcct cgttccccca cggcgaaagc tgcagcgccg gaagtgtgcc gggaccgcgg
     3301 cgttacgcaa tgggtgtcgg acgatctccg acaaattccg acgaagtgtg gatttgccgc
     3361 aacaaccaat gctggttcgc gccccggtcg ctaaccgtgt ttgaagtgct tggacagtcg
     3421 caggccctgg cgctgatagt tggaaccaat tccctgaccg tagacctccg gcggaggctc
     3481 ggtcagccgc tcgaaaacca gcctgccaat gatctggccg tgttcgagga tgaagggcac
     3541 ctcgcggctt ctcacctcga gcacggcgcg cgcgccgcgc ccgaggccgg cttcgtgccc
     3601 gaagccggga tcgaagaagc cggcatagtg cactcggaat tcaccgacca gcggattaaa
     3661 gggcagcatc tcggcagcga actccggcgg gatggacacc gcctcgcgcg aggcgagaat
     3721 gtagaactcg tccggatcga gcacgatcgt gccggaccgg ctggcctgga tcgagtccca
     3781 gaaatccgca gcctccagtg cgtcccgccg atcgatatcg atgatccccg tgtgacgctt
     3841 ggcacgccac cccacttgtt gcagcgcggg atcacccttg aggtcgacgg acagcgccag
     3901 cccgttttcg atatcggccg ggccgtcgac cagcggcgta tcggcgtgca gccgcttcag
     3961 ctgcgtgtcg gtgaattgcg gcgaaccgcg ccggatgcgc agctggttca gccgcgatcc
     4021 cttgcgcacc aggatcggaa aggtccgcgg cgaaatctcg gcatagagcg gcccccgata
     4081 gcccggctcg acgcggtcga aagactgcgc ccggtcgaca atgagacgtg tgaagacgtc
     4141 gagccgcccc gtcgagctct tgggattggc gacgcccgag acgcggaaac tgagctcgag
     4201 gctctcgagc agaggcacga catagacgca gccggtttcg agcaccgcgc cgtcggtcag
     4261 atcgatctcg tgcagtgcaa ccgtagcgag cttgtcgcgc accgtcgcct gcgggccggg
     4321 caaaaagctt gcccgcacgc gccaggccat tggcccgagc ctgaggtcca cgctcgcagg
     4381 ctgaatttgc gagggctcga tctcctccgc cgccagcacc tcgcgccgcg cccggataag
     4441 ctgtttcagc acatggctcg gcacgatg
//
LOCUS       DASZMC010000424         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4315415,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000424 DASZMC010000000
VERSION     DASZMC010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4315415"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VG274_08765"
     CDS             complement(<1..569)
                     /locus_tag="VG274_08765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWE06786.1"
                     /translation="MAEFDVDLFVIGGGSGGVRAARIAAGHGARVMIAEEYRMGGTCV
                     IRGCVPKKLFVLGSHVHQEIEDAAGFGWTIPAPSFDWPTLIANKDKEIARLEAAYTTN
                     VEKSGAKVLKTRAVLEDAHMLRLANGETVKAGYVLIATGGAPNHGAMIPGIEHVISSN
                     EAFHLPALPKRIVIQGGGYIALEFACIFAG"
     gene            complement(607..1143)
                     /locus_tag="VG274_08770"
     CDS             complement(607..1143)
                     /locus_tag="VG274_08770"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HWE06787.1"
                     /translation="MKSVLRILPAVALALGLALASTPAGAQQTPGPAAPALKQGTPAC
                     MAAARQILQMKNAGAMYAQAVPNIVAQTKDQLLSTNLNLQKDLNEVAVIVAQKLAGRE
                     KEIGEGMAQIYCNEFTEKDLTDLVAFYKSPLGQKLLLSEPRAIQFSMSYMNGWAHNFA
                     ETVNGEFRAEMRKRGKQI"
     gene            complement(1170..1862)
                     /gene="rpiA"
                     /locus_tag="VG274_08775"
     CDS             complement(1170..1862)
                     /gene="rpiA"
                     /locus_tag="VG274_08775"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWE06788.1"
                     /translation="MDELKRLAAARALEEVRDGMKLGLGTGSTAKHFVELLGERVRSG
                     LKVVGVPTSETTRRDAEASGIALATLDDIDRLDLTVDGADEIDPALNLIKGGGGALLR
                     EKIVAAASDRMIVIADDSKCVEVLGRFPLPVEVIPFGLAATQRGMARAFAQCGASGPM
                     VLRKGRDGHVFVTDGGHWIVDAHLGRIEDAPRLAGYLGAIPGVVEHGLFTNLASVAVL
                     AGGQGIRVVERR"
BASE COUNT          354 a          682 c          602 g          387 t
ORIGIN      
        1 cctgcaaaaa tgcaggcaaa ttccagcgcg atatagccgc cgccctggat cacgatgcgc
       61 ttcggcagtg ccggcagatg aaacgcctcg ttcgaggaaa tcacgtgctc gatgcccggg
      121 atcatcgcac cgtgattggg cgccccgcct gtcgcgatca ggacataccc ggctttgacg
      181 gtctcgccgt tggccaggcg aagcatatgg gcatcttcca gcaccgcgcg ggtctttagg
      241 accttggcgc cggacttctc gacattggtg gtgtaggccg cttccagccg cgcgatttcc
      301 ttgtccttgt tggcgatcag cgttggccaa tcgaagctcg gcgcaggaat cgtccagcca
      361 aagccggcgg catcctcgat ctcctgatgg acatgggagc cgagcacgaa cagcttcttc
      421 ggcacgcagc cgcggatcac gcaggtgccg cccatccggt actcttcggc gatcatgacg
      481 cgggcgccgt ggcccgccgc gatccgggcg gcacgcaccc cgcccgaacc ccctccgatg
      541 acaaacaggt cgacgtcgaa ttcagccatt tgtccctcga cctttattag atattagagc
      601 agtcgatcag atctgcttgc cgcgtttgcg catttcggca cgaaactcgc cgttgacggt
      661 ctcggcgaag ttatgggccc aaccgttcat ataggacatg ctgaactgaa tcgcgcgggg
      721 ttcgctcaaa agcagtttct ggccgagcgg cgatttgtag aaggccacga ggtccgtcag
      781 atccttctcg gtgaactcgt tgcagtaaat ctgcgccatg ccctcgccga tctccttttc
      841 gcggccggcg agcttctggg cgacaatcac agcaacctca ttgagatcct tctgaaggtt
      901 caggttggtc gacagcaact ggtccttggt ctgcgcgact atattgggaa cggcctgggc
      961 gtacattgcc cctgcattct tcatctgcag aatttgccgg gccgccgcca tgcaggccgg
     1021 tgtgccctgc ttgagcgccg gggcggccgg gcctggggtt tgctgcgcac cggccggcgt
     1081 gctggccaag gccaatccca gcgcgagagc tacagccggc aaaattctca aaacactctt
     1141 cattccgctt ctccttggga cttgcggcgt cagcgccgct caacaacgcg aattccttgc
     1201 ccgcccgcca gaacggccac actcgccagg ttcgtgaaca gcccgtgctc gacgacgccc
     1261 gggatggcgc cgagatagcc tgccagacgc ggggcatcct ctattcgacc gagatgggca
     1321 tcgacaatcc agtggccgcc atcggtgaca aaaacgtggc catcccggcc cttgcggagc
     1381 accatgggcc cggaagcgcc acattgggca aacgccctgg ccatgccgcg ctgggtcgct
     1441 gccagcccga acgggatgac ctccaccggt agcgggaagc ggccgagcac ctcgacgcat
     1501 ttgctgtcgt cggcgatcac aatcatgcga tccgaggctg ccgccacgat cttctcgcgc
     1561 agcagcgcgc cgccgccacc cttgatcaga ttgagcgcgg gatcgatctc gtcggcacca
     1621 tcgaccgtca ggtcgagccg gtcgatgtcg tcgagcgtgg cgagcgcaat gccgctggct
     1681 tccgcatcgc ggcgggtcgt ctccgaggtc ggcacgccga ccaccttgag cccggagcgg
     1741 acccgctcgc ccagcagctc gacaaaatgc ttggcggtcg agccggtgcc gagcccgagt
     1801 ttcatcccgt cgcgcacctc ttcgagcgcg cgcgccgccg ccagccgttt caactcgtcc
     1861 attgtcacgc gaaaacctgc ccggaacggt ttggcggggg ttctagcctc gttttcggac
     1921 cgggaatagc ccgccaatcg gtccgatgct tcgccctttt ggccttttta gccggccgtc
     1981 acccggtcca tcgcggcctg gatcgcggca aatcgcccat ccacc
//
LOCUS       DASZMC010000425         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_171726,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000425 DASZMC010000000
VERSION     DASZMC010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171726"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1049)
                     /locus_tag="VG274_08780"
     CDS             complement(183..1049)
                     /locus_tag="VG274_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06789.1"
                     /translation="MAALPTVKIAPNNPVFDLPVIVGIEEGLFAKAGLDVSFSATYAD
                     REKDSVERPIMARLKEQMFDCGSADSYNVCEWASIDRLERGKRGGNIAALRASVAAQA
                     ILSFDDALQAPRDLADVPVMVQELTGSHYTTVQMLESAVGAEHVKIEKGGLPQMRYAA
                     LKNHSTRAIAVMEPFISLGLKEGAHIIAASFYRGGEVISPDLTPEERKAYYDAENLAV
                     DMINADFYKYAHHVTAHAKGALEPRELLRAFVHYKHVDYYDPALFNRAYDWMKAHGLS
                     EGQSQHAALVVG"
     gene            complement(1277..1789)
                     /locus_tag="VG274_08785"
     CDS             complement(1277..1789)
                     /locus_tag="VG274_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWE06790.1"
                     /translation="MSTAKILFAALLLGSTSAALASDLPGLGKPISEADLAVWDISVG
                     PDGKGLPAGSGTPQQGAAIFAQKCAACHGEGGTGGSNGALINAPGKNARTMALYVPHA
                     TTVFDFIHRAMPWPQPKSLSYDEVYALTAFILAGNKIIGENDTMNAETLPKVRMPNRD
                     GFVSRYPEKH"
BASE COUNT          300 a          550 c          581 g          390 t
ORIGIN      
        1 tgcatttcgg tgtctttgtt gatcgcccga tcgacccgga tgagttgccc ggccttttcc
       61 agtgcgcgca catgatcgtg caggtccgga taggcgcggc gagaggcggg gagagctccc
      121 cgcttcgttt tggtaatggc gtccatctat tcaggttcgc tttagctttt agccgacccc
      181 attcacccga ccaccagcgc cgcatgctgg ctctggcctt cgctcaagcc atgcgccttc
      241 atccaatcat aagcgcggtt gaacagcgcc ggatcgtagt agtcaacatg cttgtagtgc
      301 acgaaggcgc ggagcaattc gcgcggttcc agcgcgccct tggcgtgcgc cgtgacgtgg
      361 tgagcgtatt tgtagaaatc ggcattgatc atgtcgaccg ccaggttctc ggcgtcgtaa
      421 taggccttgc gctcttccgg tgtgagatca ggcgagatca cctcgccgcc gcgatagaaa
      481 gacgccgcga tgatatgcgc gccttccttc aggccgaggc tgatgaacgg ctccatcacg
      541 gcgatcgccc gggtagagtg gttcttgagc gccgcatagc gcatctgcgg caggccgccc
      601 ttctcgatct tgacatgctc cgcgccgacc gcgctttcga gcatctgcac ggtggtgtag
      661 tgcgagccgg tgagctcctg caccatcacg ggcacatcgg cgaggtcgcg cggcgcctgc
      721 agggcgtcgt cgaacgaaag gatcgcctgt gcggcgaccg aagcgcgcaa ggcagcgatg
      781 ttgccgccgc gcttgccgcg ctcgaggcgg tcgatgctcg cccattcaca gacattgtag
      841 ctgtcggccg agccgcaatc gaacatctgc tctttcaggc gcgccatgat cggccgttcg
      901 acactgtctt tctcgcggtc ggcgtaagtg gccgagaagc tgacatcgag gcccgccttg
      961 gcgaacaggc cttcttcgat gccgacaatg accggaaggt cgaagaccgg attgttcggc
     1021 gcgattttga cggtggggag cgcggccatg ttgttcctcg tgttttgact ttggttttcg
     1081 tttttcgttt cccgcccgaa ttgatccaac gtcattgccg ggcttgaccc ggcaatccat
     1141 cttcttgcga aaagatggac ccgcgggtca taagggcgtt tacgcccgtc tttcgacggg
     1201 ctatgcccgc gggtgacgac cgaagtgaac ccgcgggtga cgaccgaagt gaatctgttc
     1261 gatcgggcat gctgaatcag tgcttctccg gatatcggct cacgaagccg tcgcggttgg
     1321 gcatgcgcac cttgggcagc gtctcggcgt tcatcgtgtc gttctcgccg atgatcttgt
     1381 tgccggcaag aatgaaagca gtgagcgcat agacctcgtc gtaggacagc gacttgggct
     1441 gcggccacgg catcgcccgg tggatgaagt cgaagaccgt ggtcgcgtgc ggcacataca
     1501 gcgccatcgt gcgcgcattc ttgccgggcg cgttgatgag tgcgccgttc gagccgcccg
     1561 tgccgccttc gccatgacac gctgcacatt tctgggcgaa gatcgcagcg ccctgctgcg
     1621 gcgtgccgct gcctgcgggc aggcctttgc cgtccggtcc gacgctgatg tcccagaccg
     1681 cgagatcggc ctcgctgatg ggtttgccga gaccgggcag atccgatgcg agcgcggccg
     1741 aggtgctgcc caggaggaga gctgcgaaga ggattttagc cgtagacatg cttgacctcc
     1801 ccttggctgt cgatcgccca g
//
LOCUS       DASZMC010000426         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2388403,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000426 DASZMC010000000
VERSION     DASZMC010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388403"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VG274_08790"
     CDS             <1..415
                     /locus_tag="VG274_08790"
                     /inference="COORDINATES: protein motif:HMM:NF018037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HWE06791.1"
                     /translation="ESLKTAAPAGYSICNDGQSEIWAAIALRSRRISVVRGWWDVPSG
                     ACAKVLTTPLDYDNVYLLAQKHGNDKLVSGPTAFCVSGNMFEIYGNAPCTAAGLSTVG
                     FASTNTKDVTGLVVHVGNDGLLPPAPKWTPAHAAK"
     gene            complement(567..1370)
                     /locus_tag="VG274_08795"
     CDS             complement(567..1370)
                     /locus_tag="VG274_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWE06792.1"
                     /translation="MRARRVAAVALRQFYLFSGSLTRVVPLFAWVAIDITLWGFISRY
                     LNSVALPGYNFIPALLGAVLLWDFLARVAQGVSTAFLEDVWSRNFLNLFATPLQISEY
                     ICGLVITAIATSLIGLLTMLALAAAAFGLSFVVYGAALVPFVLVLFLCGIALGIAGSA
                     MVLRLGPASEWFLWPMPALIAPFAGVYYPVSSLPGWMQAISRLLPPSYVFDGMRAIVR
                     HARWDVRSLAIGLTLSTLYIVIAAIFFRQVYRFAVRTGLLARYNAENPS"
     gene            complement(1370..2167)
                     /locus_tag="VG274_08800"
     CDS             complement(1370..2167)
                     /locus_tag="VG274_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE06793.1"
                     /translation="MTAANRNDPRPEPVEGRAVPPVLAVSDLRKTYRETIAVDGVSFA
                     IPPGEIVGLLGPNGAGKTTIVNMILGVLEPNAGSIRIDGIDLLRRRSAALAFTNFSAV
                     YAALPGNLTVEQNLRVFGLIYEVPALAERIEDLLVRFDLVRFRGAKSGVLSSGEQTRL
                     SLAKALLNRPKLLLLDEPTASIDPSAAREIRATIRAFADENASAVLWTSHNMYEVEEV
                     CHRVLFLSRGRVLLEGDPKLLPREHGAATLEDLFVQVARERLHPEHS"
BASE COUNT          510 a          776 c          846 g          410 t
ORIGIN      
        1 cgaatcgctc aagaccgccg cgccggccgg ctattccatc tgcaatgacg ggcagtcgga
       61 gatctgggcg gcgatcgcgc tgcgctccag gcgcatcagc gtcgtgcgcg ggtggtggga
      121 tgtgccgtcg ggcgcctgcg ccaaagtcct gacgacgccg ctcgactacg acaacgtcta
      181 tctgctcgcc cagaaacacg gcaacgacaa gctggtcagc ggcccaacgg cgttctgcgt
      241 ctcgggcaac atgttcgaga tctacggcaa tgcgccgtgc accgcggccg gattgtccac
      301 cgtggggttc gcgtccacga acacgaagga cgtcaccggt ctggtcgtcc atgtcggcaa
      361 tgacggcctt cttccgcccg cccccaaatg gactcctgcc cacgccgcga agtaacccac
      421 aaagacaagt tttaacgcag ggggcgcgga ggtaagcgca agggacgcgg aggaagatat
      481 ttggcgcggc caaaagcgcg cgattctttc agcgaccacc gcgcatcctc cgcgcgccct
      541 gcgttaaatc ttccttcttc gttcgttcag ctggggtttt cggcgttgta gcgggcgagg
      601 aggcccgtgc ggacggcgaa gcgatagacc tggcggaaga agatcgcggc gatcacgatg
      661 tagagcgttg aaagcgtgag gccgatcgcg aggcttctca catcccatcg cgcgtgacgg
      721 acgatggcgc gcatgccgtc gaaaacatag gaaggcggca gcagccgcga gatcgcctgc
      781 atccatcccg gcaagctgga gacgggataa tagacgcctg cgaacggcgc gatcagcgcg
      841 ggcatcggcc agaggaacca ttccgatgcc ggacccagcc gcagcaccat cgcgctgccc
      901 gcgatgccga gcgcaattcc gcacaggaac agcaccagca cgaagggcac cagcgccgcg
      961 ccgtaaacga caaaggaaag accgaacgcc gcggccgcca gcgccagcat ggtgagaagc
     1021 ccgatgaggc tcgtcgcgat agcggtgatc accagtccgc agatgtattc cgatatttgc
     1081 agcggcgtcg cgaacaggtt gaggaaattc cgcgaccaga catcttcgag aaatgccgtc
     1141 gacacgccct gcgccacgcg ggcaagaaag tcccacagga gcacggcgcc gagcagcgcg
     1201 gggatgaaat tgtagccggg cagcgcgacg ctgttcagat agcgcgagat gaatccccaa
     1261 agcgtgatgt cgatggcgac ccaggcgaag agcggcacca cgcgggtgag gcttcccgag
     1321 aaaaggtaga attgcctgag cgccacggcg gcgacgcggc gcgcgcgcat cagctatgct
     1381 ccgggtgcag ccgttcgcgc gccacctgca cgaacagatc ctcgagcgtc gccgcgccgt
     1441 gctcgcgtgg aagcagtttc ggatcgcctt cgagcagcac gcgcccccgc gacaggaaga
     1501 ggacgcggtg gcagacttcc tcgacctcgt acatgttgtg gctggtccac agaaccgcgc
     1561 tggcattctc gtcggcaaag gcgcgaatcg tggcgcggat ttcgcgcgcc gccgagggat
     1621 cgatcgaggc ggtcggttcg tcgagcaaca ggagtttcgg acggttgaga agagcttttg
     1681 cgagcgacag ccgcgtctgc tcgcccgacg acaggacgcc gctctttgcg ccgcgaaacc
     1741 gcacgagatc gaatcgaacg aggagatcct cgatgcgctc ggcgagcgcc ggcacctcgt
     1801 agatcaggcc gaacacgcgc aggttctgct cgacggtcag gttgccggga agggcggcgt
     1861 agaccgcgga gaagttggtg aaggcgagcg ctgcgctgcg gcgcctcaga agatcgatgc
     1921 cgtcgatgcg gatggagccg gcgttcggct cgagaacgcc gaggatcatg ttgacgatgg
     1981 tggtcttgcc ggcgccgttc ggtcccagga gacccacgat ctcgcccggc ggaatcgcga
     2041 aggaaacgcc atccaccgcg atggtctcgc gataggtttt gcgcagatcg gagacggcaa
     2101 gaacgggcgg aactgcgcgc ccttcgacgg gctcagggcg aggatcgttc ctatttgcgg
     2161 ccgtcatcgt tcgttgccga gggcatcaat ggaattcctc atgctgagct tgtcgtagtt
     2221 cgacgggctc actatgagga cttaaccctc atgctgagct tgtcgaagca tgggcttgcg
     2281 gagtgttgtg ccatcgacca tgttccccgc gcaatgcggt acgcgaaagc gttagatcgg
     2341 agacctgact tgcggcacgt cggagtccag atcttcgatc tcgacaacat gctcggagct
     2401 tgagatgagc gccgatctgg cacgcatcat cgacgaggca tgggaacggc gggaatctct
     2461 cgatgcttcg acgagaggcc ctgttcgcga gacggtggag cagacgctcg acgcgctgga
     2521 ttccggccgc ctccgcgtgg cc
//
LOCUS       DASZMC010000427         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5404398,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000427 DASZMC010000000
VERSION     DASZMC010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5404398"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..553
                     /locus_tag="VG274_08805"
     CDS             281..553
                     /locus_tag="VG274_08805"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HWE06794.1"
                     /translation="MSYFTAMTERTTVRLPDDLIRRAKRKAAAQGRSLTALIEDGLRH
                     MLSERAHRAGRPHPPVSSATGGLMPGVDLDDTASLQEMDDLQSMPR"
     gene            550..975
                     /locus_tag="VG274_08810"
     CDS             550..975
                     /locus_tag="VG274_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE06795.1"
                     /translation="MILPDVNVLIHAFRRDAVRHAACKSWLDALADGDARFGMSPLVL
                     SAVVRITTNARVFREPSPVEETFAFCRSLLSLPHCERVAPGERHWNIFERLAREAAIR
                     GPRITDAWFAALAIEQGCTWITYDRDYARFPGLDWREPD"
     gene            complement(1071..1520)
                     /locus_tag="VG274_08815"
     CDS             complement(1071..1520)
                     /locus_tag="VG274_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE06796.1"
                     /translation="MAGIGSKAEISIGVLARESAVHAETIRYYEKIGVMPKPERSAGG
                     YRLYTVVHLKRLAFIRRGRELGFSLDKLREMLRLVDRHAYTCAEVRDLMRDHVAAIHR
                     KIADLRRLERVMTGIASRCSGRKVPDCPIIDALFDDGSAGTVPQAGS"
     gene            1621..1968
                     /locus_tag="VG274_08820"
     CDS             1621..1968
                     /locus_tag="VG274_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06797.1"
                     /translation="MNEKPKILGVTGGAVSLSALALAFGACCVSPWAVTLLGVSGAVL
                     LARLAVVQPYVVALTLVLVGLGFWFAYRRAPSVRGAACAAGNRLGLQWLVWIAALIVI
                     AADVVSFAPLFLS"
     gene            1980..2621
                     /locus_tag="VG274_08825"
     CDS             1980..2621
                     /locus_tag="VG274_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06798.1"
                     /translation="MPKSPILSILALGIGMIVVASCGRDAVMAKAGGNVGYAVLDARD
                     QPLRDDFNRDKGFIRLMFLVDPRCPDCLRGLADMGDDVLARLPGSARVRVYVVHEPVI
                     GGTDKDIPSAAELLRTSLARHYWNPTGDFGRQMSHVLGYRNGARWIYAWDTWLIYPAD
                     AVWRGTNPPQPVFLMHQLGGLEGNPRFQRLDGNVFAAKVDAMLAGLNDQSAPR"
     gene            2636..2860
                     /locus_tag="VG274_08830"
     CDS             2636..2860
                     /locus_tag="VG274_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HWE06799.1"
                     /translation="MPALISELTCPTCGARHTETMPSDACICFYECRDCGTVLRPKSG
                     DCCVFCSYGSLPCPPVQIERAGGSNERCCR"
     gene            2841..3260
                     /locus_tag="VG274_08835"
     CDS             2841..3260
                     /locus_tag="VG274_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06800.1"
                     /translation="MRGAADDNSRPHAGRTEERLGEPKNDWVSRPLSMVLWWCLPLGV
                     GFSANAFAMPPRAAALVWMAAFAWMGTGCVLNAIRCRRLHCYISGPAFLLGALAAGLF
                     GSGALALGPHALNNIVTATLGIVLLSFVPEIAWRKYA"
BASE COUNT          556 a         1042 c         1066 g          631 t
ORIGIN      
        1 cagcgatttc cgcgagatct atcccaccag gttctcatgg gcgcgttcgc acgatggccg
       61 tattcgccgg cacgcgtagc gcgcgcgcga catctcccgc cgccatgccg tccgggccgg
      121 ccttcaccac caggcggaag acctcgaggc gcttcctgcg cgattgcggc ggaggcgctt
      181 ggcagccgtc tcaaaatcca tatttccata atcgcagaaa tattgaattt tatcagccat
      241 cgaccggccg cagcctggac atattgacat atcgtggacg atgtcttatt tcacagcgat
      301 gacagagcgc acgacggttc gccttcccga cgatctgatc cgccgcgcca agcgcaaggc
      361 ggccgcacaa ggccggtcgc tcaccgcgct gatcgaagac gggctccgcc atatgctgag
      421 cgagcgggcg caccgcgccg gccgcccgca tccgcccgtc agttcggcga cgggggggct
      481 gatgcccggc gtcgatctgg acgacacggc ctcgcttcag gaaatggacg atctgcagtc
      541 catgccacgt tgatcctgcc cgacgtcaat gtcctgatcc acgccttccg cagagatgcc
      601 gtgcggcatg ctgcatgcaa gtcctggctc gatgcgcttg ccgatggcga tgcccgattc
      661 ggcatgtcgc ccctcgtcct ctccgcggtg gtgcggatca ccaccaatgc gcgggtgttt
      721 cgtgaaccga gcccggtcga ggaaaccttc gccttttgcc gcagtctgct ctcgctgccg
      781 cattgcgagc gcgtcgcgcc tggcgagcgg cactggaaca ttttcgaacg gctcgcgcgc
      841 gaagcggcca tccgcggacc gcgcatcacc gatgcctggt tcgccgcgct cgcaatcgag
      901 cagggatgca cctggatcac ctatgaccgc gactatgcgc gctttcccgg actggactgg
      961 cgcgaaccgg actgaagaca tccctgggac cgcgggcgtc tcgcccgcaa ggccgagcgc
     1021 gcggcacgcg cgcggttcca gggttcagcc tcgccgcgca ccggtcgcca tcacgaccct
     1081 gcttgcggta ccgtcccggc agagccgtca tcgaacagcg catcgatgat gggacaatcc
     1141 ggaaccttgc gtccgctgca gcgcgacgca atgcctgtca tcacccgttc cagacgccgc
     1201 aggtcggcaa tcttgcggtg aattgcggcg acatgatcgc gcatcaggtc gcggacttcg
     1261 gcacaggtgt aggcatgtcg gtcgacgagg cgcagcatct cgcgcagctt gtcgaggctg
     1321 aaaccaagtt cgcgcccgcg gcggatgaag gcgagccgtt tgagatggac caccgtgtag
     1381 agccgatagc ctcctgcact gcgctcgggc ttcggcatca cgccgatctt ctcgtaataa
     1441 cgaatggtct cggcatggac ggcgctttcc cgcgcgagca cgccaatgga aatctccgcc
     1501 ttgctcccga ttccggccat ggaaaaaact ccttgaccct gtagctgcta cagggtccag
     1561 ggtcagatta agccgcaaag gcgcgccacg gcgataaaag cggcgccaaa gagaccggac
     1621 atgaatgaaa aaccgaagat tctcggcgtg accggcggcg cggtaagcct gagcgcgctg
     1681 gctctggcgt tcggcgcctg ctgcgtgtcg ccatgggcgg tgaccctgct tggtgtttcg
     1741 ggcgccgtgc tgctggcgcg gctcgctgtt gtgcagcctt atgttgtggc actgacgctc
     1801 gtgctggtgg gcctcggatt ctggtttgct taccggcgcg cgccttcggt gcgcggtgca
     1861 gcgtgtgcgg ccgggaatag attggggctg caatggctgg tgtggatcgc agccctgatt
     1921 gtcatcgccg cagatgtcgt atcctttgcg ccattgttcc tgtcgtgagg agaaacgcga
     1981 tgccgaaatc accaatcctg tcgatactcg cactcggcat cgggatgatt gtggtcgcga
     2041 gctgcggccg cgatgcggtc atggcgaagg ctggcggcaa tgtcggctac gcggtgctcg
     2101 acgcaaggga ccagccgctg cgcgacgatt tcaaccgcga caagggcttc atccggctca
     2161 tgttcctcgt ggacccgcgc tgcccggatt gtctgcgcgg actggcggac atgggcgatg
     2221 acgtacttgc caggttgccg gggagcgcgc gcgtgagagt ctatgtcgtg cacgagccgg
     2281 tgatcggcgg caccgacaag gacatcccgt cagccgccga attgctgcgc acgtcgctgg
     2341 cgcggcacta ctggaatccc accggagact tcgggcggca gatgtcgcat gtcctggggt
     2401 accggaacgg cgcgcgttgg atctatgcat gggacacatg gttgatttac cccgcagatg
     2461 cggtgtggag aggaacgaat ccaccgcaac ccgtcttcct gatgcatcaa ctgggcgggt
     2521 tggaaggcaa tccgaggttt cagcgcctgg acggcaacgt gtttgcggca aaggtcgatg
     2581 cgatgcttgc agggctcaac gatcaaagcg cgccgcggtg acgacaagga ccgctgtgcc
     2641 ggcgctcatt tccgaactga catgccccac atgcggcgcg cgccacaccg agacaatgcc
     2701 gagcgacgcc tgcatctgct tctacgaatg ccgggactgc ggcacggtgc tgcgccccaa
     2761 atccggcgat tgctgcgtgt tctgttccta cggatccttg ccctgcccgc cggtccagat
     2821 cgagcgggcg ggcggatcga atgagaggtg ctgccgatga caattcccgc ccgcatgccg
     2881 gccgaaccga agaacgattg ggtgagccga agaacgattg ggtgagccgg cctctttcca
     2941 tggtcctgtg gtggtgcctt ccgttgggcg tgggattttc cgcgaatgcg tttgcgatgc
     3001 cgccgcgagc ggccgccctt gtgtggatgg ccgcgttcgc gtggatgggg acgggatgcg
     3061 tgctcaatgc gatccgctgt cgccggctgc actgttacat ctccggaccc gcgttcctcc
     3121 tgggagccct tgccgccgga ttgttcgggt ctggcgcttt ggcgttgggc ccgcacgcgc
     3181 tcaacaacat cgtcacggca actctgggaa tcgtcctgct gtcgttcgtg cccgagatcg
     3241 catggcgcaa atacgcctga tgcaggcgcg gctctcgtct tcagatcgac gagag
//
LOCUS       DASZMC010000428         5298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_473975,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000428 DASZMC010000000
VERSION     DASZMC010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473975"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..254)
                     /locus_tag="VG274_08840"
     tRNA            complement(180..254)
                     /locus_tag="VG274_08840"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(220..222),aa:Val,seq:gac)
     gene            complement(458..817)
                     /locus_tag="VG274_08845"
     CDS             complement(458..817)
                     /locus_tag="VG274_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE06801.1"
                     /translation="MARILLAEDDESLRRFLAAALVKAGHEVTDFGDGEQAHECLTRA
                     SFDLLLTDIVMPGLDGIELAKRAAELDPGLKIMFITGFAAVALHPSSNAPKHARVLSK
                     PFHLREIVQEIDRMVAA"
     gene            986..1894
                     /locus_tag="VG274_08850"
     CDS             986..1894
                     /locus_tag="VG274_08850"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HWE06802.1"
                     /translation="MRNLAGYRAERDPVLDCLCAEPVRLIRPARQSSPFVFASPHSGR
                     LYPASFVERSRLNRTTLRRSEDAFVDELFSAVVALGAPLILARFPRAYLDANRAPAEL
                     DAAMFEGALPMTVDRASPRVHAGLGIIPRVVREGVEIYREKLTAREAGERIARFYRPY
                     HAALDRLVAETRARFGAAIVIDCHSMPSAAAAPDIILGDRLGLAAAAEVTSAAEFAFG
                     GAGFRIARNAPYAGGHTTLLHGRPGTGIHAVQIEINRGLYLDEERVERGLRFAETRRR
                     ISGALRDLIGCAACLARAPAAPLAAE"
     gene            2962..3870
                     /locus_tag="VG274_08855"
     CDS             2962..3870
                     /locus_tag="VG274_08855"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWE06803.1"
                     /translation="MAELALDRTLPPSAAAWTRRPPQRTIMRSAPREPLRPMRRHRTI
                     FISDVHLGTRGCKAELLADFLVRNSCETLFLVGDIVDGWRLKRRWYWPDAHNRVIEAL
                     LRKIDAGTRVVYVPGNHDEVLRSYCGRSIAGVEVTYEAIHETADGRQLLVLHGDRFDA
                     VIACAKWLAHLGDGAYTLALQLNEVCHVLRRALNLPYWSLSAWLKGKVKNALEYICRF
                     EEAVAREVRARGFDGVVCGHIHHAAIKTIEGVLYVNDGDWVESCTALVEDMRGNLEIL
                     CWASAGAKEPAAKGSVQHALAAAIPA"
     gene            3881..5029
                     /locus_tag="VG274_08860"
     CDS             3881..5029
                     /locus_tag="VG274_08860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWE06804.1"
                     /translation="MTVDAAPAGPGARASSAARAGRLLIFTDAWAPQVNGVVRTLETL
                     GRDLEALGCEVRYATPKGRLTVPLPTYPEIRLALFPRRSLERTIDEFAPDAIHIATEG
                     TIGLSARAICRARGLPFTTSFHTRFPEYVHARFPFVPKQFVYRFLRWFHGPATAMMVA
                     TPSLKQELEANGFGNVRIWARGVDVAQFRPIAGASLPFPRPIWLYVGRIAVEKNIETF
                     LKLDLPGTKVIIGDGPARSSLARAYPDARFLGLKSGEELVRHYAASDVFVFPSLTDTF
                     GLVMLEALACGVPVAAFPVQGPIDVLGDAPIAALDRDLARACLRALEIPRLAARAFAL
                     TRSWRSCTEQFLSNLPVRFPIREVEAESPAPDVMLSAAATPEPLRVVS"
BASE COUNT          927 a         1706 c         1652 g         1013 t
ORIGIN      
        1 ctcagtggtt cttaaatgcc taagcgtcgt cgatgcgcac gtaacccgta ggaattttca
       61 ttgcgcttgt tcaggttcta ttttcgcgcg attgcttccc ccctgcttcc ccggcttttt
      121 cgtgctgttt cgttcctgtc ggggcgccgc agaaaagtga accaaatcag cagcttaaat
      181 ggtgggcgcg acagggatcg aacctgtgac ccctacgatg tcaacgtagt gctctcccgc
      241 tgagctacgc gcccgctgtt tcgcgagggg cctgtcctat agcggcgcga tcccgagctt
      301 gcaatgcgca cgcgccattc cttcccgtcg tgcgggtgga ggtttgcgac gcaaagcgga
      361 gcagaccgga agggggatcc gcgcgcgggc tacttccccc tccgcccgct tcgcgggcac
      421 ctcccccatg atgggggagg aaagggatcg ctgcacttca cgcagcgacc atgcggtcga
      481 tttcctggac gatctcgcga aggtgaaagg gtttcgacag aacccttgca tgtttcggcg
      541 cgttcgatga cggatgaagg gcgaccgccg caaagccggt gatgaacatg atcttcaatc
      601 ccggatccag ctcggcggcg cgcttggcga gctcgatgcc gtcgagcccg ggcatgacga
      661 tgtcggtcag gagcagatcg aagctcgcgc gggtgaggca ttcatgcgcc tgttcgccat
      721 cgccgaaatc ggtcacctcg tggccggcct tgacgagcgc ggccgcgagg aagcggcgca
      781 gcgattcgtc gtcttcagcc aaaaggatac gcgccatgtc tgcccctcga gttactaaag
      841 cctatatcat gtcgacggta aacgtgggtt gagccctggc cgccggcctt ttgcgggttg
      901 cggtcggccg gccatccctg ccgcttgccg ggcgggcctt gttggtggtc cactcgccgc
      961 gctgaaagga gcgctgcgcc gcgaaatgag aaacctcgcc ggatatcgcg ccgaacggga
     1021 tcccgtgctg gattgtctct gcgccgagcc cgtgcgcttg attcggccgg cgcgccagtc
     1081 gagcccgttc gtcttcgcct cgccccattc cggccgcctc tacccggcga gtttcgtcga
     1141 aagaagccgg ctcaatcgca cgacgctccg gcgctcggaa gacgccttcg tcgacgaact
     1201 gttttccgcc gttgtcgcgc tcggggctcc gctcatcctc gcccgctttc cgcgcgccta
     1261 tctcgacgcc aaccgcgcgc cggcagaact cgatgcggcg atgttcgagg gcgcgctgcc
     1321 aatgacggtc gaccgggcga gcccccgcgt gcatgcgggc ctcggcatca ttccacgcgt
     1381 ggtgcgcgaa ggcgtcgaga tttatcgcga gaagctgacg gctcgcgaag ccggggagcg
     1441 gattgcgcga ttctatcggc cctaccacgc cgcgctcgac cgccttgtcg cggaaacacg
     1501 cgcccgcttc ggcgcggcca tcgtcatcga ctgtcattcc atgccgtccg cagccgcggc
     1561 gcctgatatc atccttggtg acaggcttgg acttgccgca gccgccgagg tgacctccgc
     1621 cgcggaattc gccttcggcg gcgcgggttt ccggatagcg cgcaacgcac cttacgccgg
     1681 aggccacacc acactgcttc atggccggcc gggcacaggg atccacgccg ttcaaataga
     1741 gatcaaccgc ggactctatc tcgacgaaga acgtgtggaa cgcggcctac gcttcgccga
     1801 aacgcgccgg cgcatatcgg gcgcattgcg ggatttgatc ggttgcgccg cctgtcttgc
     1861 gcgagcgcct gccgcgccgc tcgcggctga ataaaaaagg ggaattatac agctaccctg
     1921 ccagcagcgt tctacagctc atggtgagac ttgtttagca tgaaccaaca tgggggcagc
     1981 gttgcgccag cgctggttta ataagggttc gaaatactgt cttggaaacc catcatcgcg
     2041 gcggtgctat agcccctggt ggctccggtg cgatatcctt aagcggacct tgaagaccca
     2101 tcatctggct gccattgacg atcctctggg attctttcat acggcgaacc cagtcgcgct
     2161 gcgtatggca ctcctgtgaa ccaccgaaac gcgttcccgt cggcgggggt ttcatgtgac
     2221 agacaatgtc atctggattc tgggaaaagc ccgcatcaat aaccggtgcg ctcgcagcag
     2281 cttgcgccgt catggtcgcc gccggcgcac cccccgaggt taccacaaga ctgggtgccg
     2341 gcgcgggggt cgtggccaag gccgattgcg gcgcggcggc gtcggctccg ctgagttgcg
     2401 gcgccaggag cgcggttgcg gaaagcgcga ttgcggcgag aacacgcatc ctcaaagatc
     2461 ccctttggct gcgcagcagc aagcctaggg aaaaaccgta tcgagattca agacgctaca
     2521 aattcacgtt tgcgtcaact gtctttcgcc acttcgaaca aacggaatcc gtcgcctcac
     2581 ccttcgacgg gttcaggata aggcgcaaat tcgcttaaaa aaggccgcgc ctcgaaggcg
     2641 cggccaagtg aagggaggaa acgcccagga agggcaacag ccccctgcag agatgctccg
     2701 cgaaggccgt atgcggtgaa gtatgacttc ggcgatactg cgatgcaaca ataaattgtg
     2761 cgcgcgctct gttcattttt agctgtaatt tcagatagtt ccatggtgca tagccgaacg
     2821 gttgtgcgat gcggcgcaga tgtcgcaccg ccagccgggc cgggattcac aaaaccatga
     2881 ccggtccgca acacgagtgt cgcgaatgac gggcagtcag gtgcatgacc gattcgcagt
     2941 gattcgcgcg aggagcacgc gatggccgag ctggccctgg atcgaacatt gccgccatct
     3001 gcggctgcct ggacccgcag gccgccgcag cgcacgatca tgcggagcgc tccgcgcgag
     3061 ccgcttcgtc cgatgcggcg gcaccggacg atctttattt ccgacgtgca tctgggcacg
     3121 cgcggctgca aggcggaact cctcgccgat ttcctggtgc gcaacagctg cgaaacgctg
     3181 tttctcgtcg gcgacatcgt cgatggctgg cggctgaaac gccgctggta ctggcccgac
     3241 gcccacaacc gcgtcatcga ggcgctgctg cgcaaaatcg acgcgggcac gcgtgtcgtc
     3301 tacgtgccgg gaaatcacga tgaagtgctg cgcagctatt gcggacgttc gatcgccggc
     3361 gtggaagtga cgtacgaggc gattcacgag acggccgatg gcaggcagct tctggtgctg
     3421 catggcgacc ggttcgacgc ggtcatcgcc tgcgccaaat ggctcgccca tctcggcgac
     3481 ggcgcctaca ccttggctct gcagctcaac gaggtctgcc acgtgctgcg gcgcgcattg
     3541 aacctgccct actggtcgct ctccgcctgg ctgaaaggga aggtcaagaa cgcacttgaa
     3601 tacatctgcc gcttcgaaga ggcggtggcg cgtgaggtcc gcgcacgcgg cttcgacggc
     3661 gtggtgtgcg gtcatattca ccatgcggcc ataaaaacga tcgaaggcgt gctttacgtt
     3721 aacgacggcg actgggttga aagctgcacg gcgctcgtcg aagatatgcg cggaaatctg
     3781 gagattctct gctgggcatc cgccggcgca aaagagcccg ccgccaaggg ctcggttcag
     3841 cacgcactcg cagcagcgat tcccgcctga ggcagtctct atgaccgtcg atgctgcccc
     3901 tgcggggccc ggcgcacgcg cgtcttcagc ggctcgggcc ggcaggcttc tcattttcac
     3961 cgacgcctgg gcgccgcagg tcaatggcgt cgtgcgtacg ctcgaaacgc tcgggcgcga
     4021 tctggaagcg ctgggttgtg aggtgcgcta cgcaacgccc aagggccgcc tcaccgtgcc
     4081 cttgcccacc tatcccgaga tcaggctggc gctgtttccg cgccgaagtc tcgaacggac
     4141 gatcgacgaa tttgcgcccg atgcgattca catcgcgacc gaaggcacaa tcgggctgag
     4201 cgcgcgggcc atctgccgcg cgcgcggact gcccttcacg acgtcgttcc atacgcgctt
     4261 cccggaatat gtgcacgcgc gctttccctt cgtgccgaaa cagttcgtct accgcttcct
     4321 gcgctggttc cacgggccgg cgacggccat gatggtcgcg accccctccc tgaagcagga
     4381 gctcgaggcg aacggattcg gcaatgtccg catctgggcg cgcggcgtgg atgtcgcgca
     4441 gttccggccg atcgccggcg cctctctgcc tttcccccgt cccatctggc tctatgtcgg
     4501 ccgcatcgcg gtcgagaaga acatcgagac gtttctcaag ctcgatcttc ccggcacgaa
     4561 agtgatcatc ggcgatggcc ctgcccgctc gagccttgcc cgcgcctatc ccgacgcacg
     4621 attcctgggt ctcaaatcgg gcgaagaact cgtccgccat tatgcagcaa gcgatgtttt
     4681 cgtatttccg agcctgaccg acacgtttgg gctcgtgatg ctggaagcgc tggcgtgcgg
     4741 ggttcctgtg gccgcgttcc ccgtgcaggg gccgatcgac gtgctgggcg atgcaccgat
     4801 cgccgctctc gatcgcgatc tcgcccgcgc ctgcctgagg gcgctcgaga ttccgcggct
     4861 ggcggcgcgc gcctttgcac tcacccggtc gtggcgcagt tgcaccgaac agtttctttc
     4921 caatctgccc gtcaggttcc cgattcggga ggtggaagca gaatctcccg cgccggacgt
     4981 gatgctgtcc gctgctgcca cgcccgagcc gcttcgcgtt gtgtcatgag ttttccgtac
     5041 cgcctgcgca ccgggaatcc gacgcgaggt ctcataggcg cgaagataac caagcccgag
     5101 cgacccctca cccgcgccgg agcagaattc attgcacgta tcggcgtccc tctcccacaa
     5161 ggggagaggg gaagtcggag cgttgcatca aagccggaaa acaagcgcgt ggattcccct
     5221 ctccccttgc ccgccttcgc tcaagctacg gcgggtctgg cggctcactt agtccgccga
     5281 agccttggcg aaggcgga
//
LOCUS       DASZMC010000429         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_172147,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000429 DASZMC010000000
VERSION     DASZMC010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172147"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VG274_08865"
     CDS             <1..180
                     /locus_tag="VG274_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06805.1"
                     /translation="ESAIGKTAQIVLEPMQPGDVSATYADIESSRRDLGFEPTTPIRE
                     GVPRFIRWYRQHYGV"
     gene            complement(367..1842)
                     /locus_tag="VG274_08870"
     CDS             complement(367..1842)
                     /locus_tag="VG274_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWE06806.1"
                     /translation="MSFITRRKSIDTLAEGGHRLKPSLSWPHLIALGVGAIVGTGIYT
                     LTGIGAGLAGPAVILSFGLCGVICACAALCYAEMSTLMPVAGSAYTYSYAVLGELVAW
                     IIGWSLVLEYTVAAAAVAVGWSAHVSAFLAAAGITLPDALSHGALVCRHGLPLSGAFG
                     NGLFACAAFDLPAVAIGAIVTALLIIGTRESATVNLILVAIKLAALVLFVALASTHFD
                     SAHFHPFAPYGFGAGIGPDGVKRGVLAAAALIFFAFYGFDAVSTAAEETKNPARDLSI
                     GIVGSMVICTAIYVAVAAAALGAAPWREISGTGAPLVHVLELLRHPFSAQLVASAAIV
                     ALPTVILVLMYGQSRIWFVMARDGLLPERLAALHKTRGTPVAMTLFAGFWVIVLAATL
                     PLDKIALLANAGTLAAFIAVALSLIVLRLREPARPRIFRAPLGIVVAFLCIAGCVYLF
                     SNGLPHFTQAWFVIWNAGGLVLYFLYGVRRSRLALATAAAE"
     gene            complement(1930..>2328)
                     /locus_tag="VG274_08875"
     CDS             complement(1930..>2328)
                     /locus_tag="VG274_08875"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HWE06807.1"
                     /translation="CPVTAALVARCPSVLSASFSFLEPGKHIPRHRGPFRGIVRCHIG
                     LSMPRDADGRPGAILWIDDVEHRLGDGDFLVWDDTYPHEVLNSTDRLRVALLLDIWRQ
                     GMPADMFALSYLIVRAVRLGLQSRLTRFGG"
BASE COUNT          496 a          760 c          759 g          313 t
ORIGIN      
        1 gaatccgcga tcggcaagac agcgcagatc gtgctcgagc cgatgcagcc gggcgacgtg
       61 tccgccacct atgccgatat cgaatcgagc cgccgcgatc tcggcttcga gcccaccacg
      121 cccatccgcg aaggcgtccc ccgcttcatc cgctggtacc gccagcacta cggcgtttga
      181 accggcgtcg ccgatatcaa aaaaggagat ccacagggag gcagtgagac agcgagaaag
      241 tcgcgcacct aaggcgcgca atagttcccc tgccgcactg tctccttggg atcatttctt
      301 cctccgcgca accccgcgtc tccgcatgtc cgttcctgca gcacgtcaat gcggtcgcat
      361 cgtgatttat tccgctgccg ccgttgccag cgccaggcgg ctgcgccgga cgccgtagag
      421 aaaatagagc acgagtccgc cggcgttcca gatcacgaac cacgcctgcg tgaagtgcgg
      481 caggccgttc gagaacagat agacgcagcc ggcgatgcag aggaacgcca cgacgatccc
      541 cagaggcgcg cggaagatgc gcggccgcgc gggctcgcgc aggcgcagaa cgatcagcga
      601 cagcgcaacg gcgatgaagg cggcgagcgt gcccgcattg gcaaggagcg caatcttgtc
      661 gagcggcaat gtcgcggcga gaacgatcac ccagaatccc gcgaacaggg tcatcgcgac
      721 gggcgtgccg cgcgtcttgt gaagggcggc gagccgctcg ggaagaagcc cgtcgcgcgc
      781 catcacgaac cagatgcggc tctgtccgta catgagcaca agaatgacgg tcggaagtgc
      841 gacgatggcg gccgaggcga caagctgggc cgagaaggga tggcgcaaaa gctcgaggac
      901 gtgcaccaga ggcgcgccgg tgcccgaaat ctcgcgccac ggcgccgcgc caagggccgc
      961 cgcagctaca gcaacgtaga tcgcggtgca gatcaccatg gatcccacga tgccgatcga
     1021 cagatcgcgc gccggatttt ttgtctcctc cgccgcggtc gagactgcat cgaaaccgta
     1081 gaacgcgaag aaaatcagtg ccgccgccgc aaggacgccg cgcttgacgc cgtccggccc
     1141 gattccggcg ccgaacccat atggcgcaaa gggatgaaag tgcgcgctat cgaaatgggt
     1201 cgatgcgagt gcgacgaaca gcaccagcgc cgcgagtttg atcgccacca gaatgagatt
     1261 gaccgtcgcg ctttcgcgcg tgccgatgat gagcagcgcg gtcacgatcg cgccgatcgc
     1321 gaccgcgggt agatcgaatg ccgcacaggc gaacaatccg ttgccgaagg cgccggacag
     1381 aggcaggccg tgccggcaga cgagcgcgcc atggctgagc gcgtcgggca aggtgatgcc
     1441 ggcagcggcg aggaacgcag aaacatgcgc cgaccagccc actgcgacgg ccgccgccgc
     1501 gactgtgtat tcaagcacca ggctccagcc gatgatccag gcgacgagtt cgccaagcac
     1561 ggcgtaggag taggtgtagg cactgccggc cactggcatc agcgtcgaca tttccgcgta
     1621 acaaagcgcg gcgcaggcgc agatcacgcc gcacaggccg aaggagagaa tgaccgcggg
     1681 cccggccagc ccggcgccga ttcccgtcag cgtatagatg ccggttccta cgatcgcgcc
     1741 cacgcccagc gctatgagat gcggccagct caagctcggc ttgaggcggt gcccgccttc
     1801 cgccagcgtg tcgatcgatt tccgccgcgt gatgaagctc atgcgctcgc gccccccaga
     1861 tgaaaggaag ttagagccac cgtggacgga gtgccatagc gtcgcggacg cgggaacatg
     1921 tcgcgccgct cagccgccga agcgtgtcag ccgcgactgc agacccaggc gcaccgcccg
     1981 cacgatgagg tacgacaggg caaacatgtc cgcgggcatg ccttggcgcc agatatcgag
     2041 aagcagcgcg acgcgcagcc tgtccgtcga attcagaacc tcgtgcggat aggtgtcgtc
     2101 ccagacgagg aaatcgccgt cgccgagacg gtgttccaca tcatcgatcc agagaatcgc
     2161 gccgggcctg ccatcggcat cgcgcggcat gctgagaccg atgtgacagc gcacaatgcc
     2221 gcgaaacgga ccgcggtggc gaggaatatg cttgcccggt tccaggaacg aaaacgacgc
     2281 cgagagaacc gaaggacacc tggcaaccag cgccgccgtc accgggca
//
LOCUS       DASZMC010000430         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3484360,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000430 DASZMC010000000
VERSION     DASZMC010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3484360"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1253)
                     /gene="mreC"
                     /locus_tag="VG274_08880"
     CDS             complement(87..1253)
                     /gene="mreC"
                     /locus_tag="VG274_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWE06808.1"
                     /translation="MANGTQRLARGRNGNQLSLAILAVLALTIVFLGRVQPTLFDRGR
                     AYVSDRTAPILETLRMPVSATVQWTGSLAQLFTVYRENLRLKEQNARLRQWQNAALML
                     EQRLKRYQLLLNAVPNPETANVTAHVIGRSTRPFLSTMILDAGRRDRVKPGEAVVDER
                     GMIGRIFLAGDHTSWVILLTDLNSRVPVSIQPGNIQAILAGDNSSAPTLEISAQGAAV
                     TDGEQIVTSGDGALLPPGLLVGKAFRDGAVFRAALFADAGRSSDVRVLELKAIPEQPP
                     LPTKSDLPVTAAGLPPLTPPAPKVTAANPPVPAPGVASAGKPTTEPKRVHTLHAQILV
                     PSVDSSAADGEAQGTNNMQAVPPTTAPAAPPPTAQPATSPQAQPNPDNSGADNQ"
     gene            complement(1298..2341)
                     /locus_tag="VG274_08885"
     CDS             complement(1298..2341)
                     /locus_tag="VG274_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010442888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWE06809.1"
                     /translation="MFGSLLGILSSDMAIDLGTANTLVYVKGRGIVLNEPSVVATITN
                     RGGKKQVRAVGNDAKMMLGRTPGNIEAIRPMRDGVIADFEIAEEMIKHFIRKVHNRRS
                     FANPMIIVCVPSGSTAVERRAIQESALSAGARRVFLIEEPMAAAIGAGLPVSDPTGSM
                     VVDIGGGTTEVAVLSLGGIVYSRSVRVGGDKMDEAVIAYIRRHQNLLIGEATAERIKK
                     QIGSAAPPGDGTGVTMEIKGRDLLNGVPKEITITQRHIGEALAEPTSQIVEAVKVALE
                     ATPPELAADIVDKGIVLTGGGSLLANLDQVLREETGLPVSIADDPLSCVALGTGRVLE
                     NAKIMRHVLTTAF"
     gene            3034..4761
                     /locus_tag="VG274_08890"
     CDS             3034..4761
                     /locus_tag="VG274_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWE06810.1"
                     /translation="MKILPPVSISARGFLPGRWDVLAAILVLGAIVVLSDESRSLVAP
                     LSTLKSNPLSLSPWALPGYALRTTLRMLAALVVSLVFTFTYATWAAKSARAGTLLVPL
                     LDILQSIPIVGYLSITYLFFLSLAPGRVLGAELAAIFTIFTSQAWNMAFSFYQSLRTV
                     PPELEEAGRVFGLSAWARFWRIEAPFAMPSLVWNMMMSMSGSWFFVVAAEAISVGHTS
                     IALPGVGSYIALAIAHKKLGAILWAILTMLIVILIYDQLLFRPLVTWADRFRFEQEQN
                     EEPPQSWMLTMFRRSRLIDAVAAPFKAALRRSYRLAPPPIVRIPLPHSRLWTRAGDLF
                     WYGLLFVLISYAIIRVVRFVSQSIFWPDVFDATSLGLITMVRVFVLIAIASAVWTPVG
                     IYVGLRPKLARIVQPVAQFLAAFPNNLLFPVAVSAIVSWRLNPDIWLSPLMVLGTQWY
                     ILFNVVAGASGLPSELRDAGANLQVRGWLWWRKIGLPGVFPFYVTGAITASGGSWNAA
                     IVAEWVNWGDRTLRAHGLGAYIMAATMAGDYRRIVLGMAVMSALVALVNRIFWRPLYW
                     YAERKYRLT"
BASE COUNT          802 a         1567 c         1514 g          953 t
ORIGIN      
        1 ctgcgctcgg cacctccccc acgttggggg aggaaagtgg agagcgtatc gtggagcgcc
       61 atttctagca tcatccgacc aatcccctac tggttgtcgg cgcccgaatt gtcggggttg
      121 ggttgtgctt gcggcgaagt cgcgggctgc gcggttgggg gcggcgctgc cggcgccgtt
      181 gtcggcggaa ccgcctgcat gttgtttgtg ccctgggcct cgccgtccgc ggccgatgaa
      241 tcgacagatg gaaccaggat ttgcgcgtgc agcgtatgca cacgcttcgg ttcggtggtc
      301 ggcttgcctg cagatgcgac gccgggcgcg ggcacgggcg gattcgcggc cgtcaccttg
      361 ggcgcaggcg gagttagagg cggaagacca gccgcggtca ccggaagatc gctcttcgtc
      421 ggcagcggcg gctgctcggg aatcgctttg agctccagta cccgtacatc gctgcttcgg
      481 ccggcatcgg cgaaaagagc ggcgcggaag acggcgccgt cgcgaaaagc cttgcccacc
      541 aaaagaccgg gcggcagaag ggcgccatct cccgaagtga caatctgctc tccgtccgtg
      601 accgccgcac cctgcgcgga gatctcgagt gtcggtgccg acgaattgtc gccggcaagg
      661 atcgcttgaa tgttcccagg ctggatggaa accgggacgc ggctgttgag atcggtaagc
      721 aggatcaccc aggatgtgtg atcgccggcg agaaaaatgc gcccgatcat gccgcgctcg
      781 tccacgacgg cttcgccggg cttgacgcga tcgcgccggc ccgcgtcgag aatcatcgtg
      841 ctgagaaacg gcctggtcga gcggccgatg acatgggcgg tgacattggc cgtttcggga
      901 ttgggcacgg cattcaggag aagctgatag cgcttcaacc gctgctcgag catcagggcc
      961 gcgttctgcc attgccggag acgcgcgttc tgttccttca ggcggaggtt ttcccgatag
     1021 accgtgaata gctgcgccaa gctgcccgtc cactggacgg tggcgctgac cggcatgcgc
     1081 aaggtctcca gaatcggcgc ggtgcgatcg gaaacatagg cgcgcccgcg gtcgaacagg
     1141 gtcggctgca cccggccgag gaaaacgatg gtgagcgcaa gaacggcgag aatggcgagc
     1201 gacagctgat tgccgttgcg gccgcgtgca agtctctgag tcccgttcgc catttcgtca
     1261 ccaaaccgtc aggcacaccc ccaacaatca aaacaaatca gaacgccgtc gtcagaacgt
     1321 gacgcatgat tttcgcgttt tcgagaacgc gccccgtccc cagcgccacg caggagagcg
     1381 gatcgtcggc gatgctgacg ggcagacccg tttcctcgcg caatacctgg tcgaggttgg
     1441 caagcagcga gccgccacct gtcagcacga tacccttgtc gacgatgtcc gcggcaagct
     1501 ccggcggcgt ggcttcgagg gcgaccttca ccgcctcgac gatctggctc gtcggctccg
     1561 ccagcgcttc gccgatatgc cgctgggtga tggtgatttc cttgggcacg ccgttcagca
     1621 gatcgcgccc cttgatctcc atggtaacgc ccgttccgtc tccgggcggc gccgccgagc
     1681 cgatctgctt cttgatgcgt tcggcggtcg cttcgccgat caaaagattc tggtggcggc
     1741 ggatgtacgc gatgaccgct tcgtccatct tgtcgccgcc cacgcgtacc gagcgggaat
     1801 agacgatgcc gccgagggaa agcacggcca cttccgtggt gccgccgccg atgtcgacca
     1861 ccatcgagcc ggtcggatcg gaaaccggca atcccgcacc gatcgccgcc gccatcggct
     1921 cctcgatgag gaacacgcgg cgcgcgcccg cggaaagcgc cgattcctga atggcgcggc
     1981 gctccaccgc cgtcgatccc gaaggcacgc agacgatgat catcggattg gcgaaggagc
     2041 ggcgattgtg aaccttgcgg atgaaatgct tgatcatctc ttccgcgatt tcgaaatccg
     2101 cgatgacgcc gtcgcgcatc ggccggatcg cctcgatatt ccccggcgtg cggcccagca
     2161 tcatcttcgc gtcgttgccg acggcgcgca cctgcttctt gccgccgcga ttggtaatcg
     2221 tcgccacgac agacggctcg ttgagaacga taccgcgtcc tttcacatag acaagcgtgt
     2281 tggccgttcc gagatcgatg gccatgtcgc tcgacaggat gccgagaagg ctgccgaaca
     2341 tgctctctgt gcctgttcct ttcctaccgg ccctggccgg tcttcgattc cgtgaggggc
     2401 attgctcgtt cgccctcctc cgctggcttg ccgggattcc cgaggcggga aatgcccgtt
     2461 tagagagtac ccgtcccgac gcgcgaatct tgccgaaatt cgttaaagaa cgtcacggca
     2521 tcgcgtcttt attttaaagg ccggattgca ttatttcgaa aatcctctgg acaggctcgc
     2581 ccgcaagaaa ggcgaactcc gcggcccggg ggcacgcacc ccagaagccc cgaaccggaa
     2641 gagggacctg aaaccggagc tatggaacga gggaggttct gcggccgcac cggtccgcgc
     2701 ccgccccagc ccacttccca gcgccgcctc ccggcgccgg gccgccactt tgcccggaca
     2761 atctgtgtcg ctcttcttcg cgtgaattca cgcatccgtc gcgaggcttt tacgccgaag
     2821 gcgaagcgcg gcgcaatagt tcgccgcatc cgctgcaacc catgctgtgc atctcggcac
     2881 tgcacgcaat cgaattgatc gccgcgaacg cgcttcctgt cgcgcagcat tgcaacaaag
     2941 gagaatttcc ggcacgaaaa agaaagttcc gacctgggcc cgcgcgggcg cacgcgggcc
     3001 ggtcccacgg ccagaaaacg cgaaggcccg cagatgaaaa tcctgccgcc cgtctcgatc
     3061 agcgcccgcg gatttttgcc cggccgctgg gacgtgctgg ccgcgatcct tgtgctcggc
     3121 gcgatcgtcg tgctcagcga cgaaagccga agcctggttg cccccctttc gacgcttaaa
     3181 tcgaatcctc tgtcgctcag cccctgggcg ctgcccggct acgcgttgcg gacgacgttg
     3241 cggatgctcg ccgcgctcgt ggtttcgctc gttttcacct tcacctatgc gacttgggcg
     3301 gccaaaagcg cgcgggcggg tacgctgctc gtgccccttc tcgacatcct ccagtcgatc
     3361 cccatcgtag gctacctctc cattacctat ttgttctttc tctcgctcgc acccggccgc
     3421 gtgcttggcg cggagctcgc tgcgatcttc acgatcttca ccagccaagc ctggaacatg
     3481 gccttcagct tctaccagtc gctgcgcact gtgccgccgg aactcgagga ggccggccgc
     3541 gtgttcggcc tcagcgcctg ggcgcgattt tggcggatcg aggcgccttt cgcgatgcca
     3601 tcgctcgtct ggaacatgat gatgtcgatg tcggggagct ggttttttgt cgtggcggcg
     3661 gaagcgatca gcgtcggcca cacctccatc gcgcttcccg gcgtcggctc ctacatcgct
     3721 ctcgccatcg cccacaaaaa gcttggcgcc attctttggg cgatcctgac catgctgatc
     3781 gtgatcctca tttacgatca gctgcttttt cggccgctcg tcacctgggc ggaccgcttc
     3841 cgcttcgaac aggaacagaa cgaagagccg ccgcaatcct ggatgctgac catgttccgc
     3901 cgctcgcggc tgatcgacgc cgtcgcggcg ccgttcaagg ccgctctgcg tcggtcctat
     3961 cggctcgcgc cgccaccaat cgtccgcatt ccgcttcccc acagccggct atggactcgg
     4021 gcaggcgact tgttctggta cgggctcctc tttgtcctga tctcctacgc catcattcgc
     4081 gtggtccgtt tcgtttcaca gagcatattc tggccggacg ttttcgacgc gacatcgctc
     4141 ggactcatca ccatggttcg ggtgttcgtt ctcatcgcca tcgccagcgc ggtgtggaca
     4201 ccggtcggca tctatgtcgg attgcgtccg aagctcgcgc ggatcgtaca gcccgtcgcc
     4261 cagttcctgg ccgcgttccc aaataatctt ctgtttccgg ttgccgtttc ggccatcgtc
     4321 tcctggcgcc tcaacccgga catctggctg tcgccgctga tggtgctcgg cacccaatgg
     4381 tacatcctct tcaatgtcgt ggccggcgcc tccggcctgc cctcggaact gcgcgacgcc
     4441 ggcgccaatt tgcaggtgcg tggctggctg tggtggcgaa aaataggact gcccggtgtc
     4501 tttccgtttt atgtgacggg cgcgatcacc gcgtccggcg gctcctggaa cgcggcgatc
     4561 gtggcggaat gggtgaactg gggcgaccgc acactgcgcg cccacggact cggcgcttac
     4621 atcatggctg caacgatggc cggcgattac cggcgcatcg tgctcggcat ggcggtgatg
     4681 agcgcgctcg tggcgctcgt caaccgcata ttctggcgcc cgctgtactg gtatgccgag
     4741 cggaagtacc ggctgacgtg agtagtgtgg cgagtggcga atagcgagtg gcgagtggcg
     4801 agtggcgagt ggcgagtggc gagtggaaag gggtta
//
LOCUS       DASZMC010000431         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5404904,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000431 DASZMC010000000
VERSION     DASZMC010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5404904"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VG274_08895"
     CDS             <1..390
                     /locus_tag="VG274_08895"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWE06811.1"
                     /translation="DRMNIATLDGDTLRWFGVALFALGGALRIWPVYILGYRFSGLVA
                     IQPGHRLVTHGIYGTIRHPSYLGLLVNSLGWSLAFRSGVGVLLTALLIPPILARIRTE
                     EKLLRDHFGAEYDTYRARTSRLIPGVY"
     gene            781..1275
                     /locus_tag="VG274_08900"
     CDS             781..1275
                     /locus_tag="VG274_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06812.1"
                     /translation="MTDPSSSPPPDDDEPHGRWPFIRDVLVLQLKLLIGNLHNFLLIP
                     ATTIAAALDLIFRSGRHGARLYRVLEWGRQADEVIGLYGALDRRDDELKHDLTVDALV
                     SRLESAIIREYEKGGTAAGMKSAVDRALDQLHREAGTATAQAREAAQRLKDRVSSPAG
                     KDAT"
     gene            1272..>1981
                     /locus_tag="VG274_08905"
     CDS             1272..>1981
                     /locus_tag="VG274_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004571 - mannosyl-oligosaccharide
                     1,2-alpha-mannosidase activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HWE06813.1"
                     /translation="MTTPSGGPMKPVARRSLLHALTGAVAFAGLPAKARTSAHRGQDA
                     DWAKLAQDVRGEMAWAWRNYVELAFGHDQIKPVSGGMEEFFLPGGPRLGLSIVEALDT
                     LYLMGLDAELEAGVRWIEKNLHFDIDGEVQVFETNIRMVGGLLSGWLATRNRHLLDLA
                     HDLADRLLPAFAKSPTGIPYRFVNLRTGAVRDATTFPAELGTYISEFGTLSRAVCDRR
                     YYDVAKKAAKACFDRRSD"
BASE COUNT          346 a          619 c          619 g          397 t
ORIGIN      
        1 gaccggatga atatcgcgac gctggatggc gatacgctgc gctggtttgg cgttgctctg
       61 ttcgcgctcg gcggtgcgct gcgcatctgg cccgtctaca tcctcggtta ccggttcagc
      121 ggcctcgtgg cgatccagcc cggccaccgc ctggtcacgc acggaatcta cggcacgatc
      181 cggcatccga gctatctcgg actgctcgtc aactcgctgg gctggagtct ggctttccgc
      241 tcgggcgttg gcgtgttgct caccgccctt ctgatcccgc cgattctcgc ccgcatccgc
      301 acagaggaga aactgctgcg cgaccatttc ggcgccgaat acgacaccta ccgcgcgcga
      361 acgtcgcggt tgatccccgg agtgtattga gggcaggccg aataaaaagc gcgtcatccc
      421 ggccggcgcg tgatcctcgc tttcgcgcta tggcatatac aggtccggct tgtgcagcgg
      481 atgtgccggt cgaggattcg cctaacctcc cccttgtggg gaggtcgaaa aacgcccttc
      541 gcgtttttcg ggtggggtaa tagcgcatcg cacagacccc cacccgaaat cgcgtgccgc
      601 gctttcgacc tccccacaag ggggaggtta gatgaaacgt tgtccgaaat tcgcgttgta
      661 tccgctacga tgtagatgcc tttgcgcttg cgcggggatg atacgccgaa aacaaatttc
      721 tatgcctcgt tccccgcatg ccgtattaca ttcccgtcgc cagcgcttgc atctgcaatc
      781 atgacggatc cgtcttcctc tccacccccg gacgatgacg aaccgcacgg acggtggccg
      841 tttatccgcg acgtgctggt gctgcagttg aaattgctga tcggcaacct gcacaatttc
      901 cttctcattc ccgcgacgac gatcgcagcg gcgctcgatc tgattttcag gtcgggccgg
      961 catggcgcgc gcttgtatcg cgttctggaa tgggggcggc aggccgacga ggtgatcggc
     1021 ctctatggcg cgctggatcg ccgcgatgat gaactgaaac acgacctcac ggtcgatgcc
     1081 ttggtgtcgc ggctcgaaag tgcgatcatt cgcgaatatg agaagggcgg aaccgcggcc
     1141 ggcatgaaga gcgcggtcga tcgtgcgctc gaccagttgc atcgcgaagc cggcacggcg
     1201 acggcccagg cgcgcgaagc ggcgcagcgg ttgaaggata gagtttccag tcccgccggc
     1261 aaggacgcaa cgtgacgacc ccgtcgggcg gacctatgaa acctgttgcg cggcgttcgc
     1321 tgcttcacgc gttgaccggc gccgtcgcgt tcgcgggatt gcccgccaaa gcacgcacat
     1381 cagcccaccg cgggcaggat gccgactggg caaagctcgc gcaggatgtt cgcggagaga
     1441 tggcctgggc atggcgcaac tatgtcgaac tcgccttcgg ccacgaccag atcaagccgg
     1501 tcagtggcgg catggaggaa ttcttcctgc cgggagggcc gcgtctcggt ctctccatcg
     1561 tcgaggcgct cgacacgctt tatctgatgg gcctcgatgc ggagcttgag gcgggcgttc
     1621 gctggatcga gaaaaatctc catttcgata tcgatggcga agtccaggtg ttcgagacca
     1681 acatccgcat ggtcggcggc ctgctttccg gctggctggc gacgcgaaac aggcatcttc
     1741 tcgatctggc gcatgacctg gccgatcggc tccttcccgc ctttgcgaaa tcgccgacgg
     1801 gaattcccta tcggttcgtc aatctcagaa ccggggcggt gcgcgacgca acgacattcc
     1861 ccgccgagct cggcacctat atttcggagt tcggaacgct ctcgcgcgcc gtctgcgacc
     1921 ggcgttacta cgatgtggcg aagaaggccg cgaaggcgtg cttcgaccgg cgctcggaca
     1981 t
//
LOCUS       DASZMC010000432         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2100875,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000432 DASZMC010000000
VERSION     DASZMC010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100875"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VG274_08910"
     CDS             complement(<1..261)
                     /locus_tag="VG274_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HWE06814.1"
                     /translation="MKLRLWQIDAFAEKVFEGNPAAIVPLPDWLEPGLLQQIAGENNL
                     AETAFLVRRESGRYDLRWFTPTTEVDLCGHATLASAWLLFAEL"
     gene            complement(438..1334)
                     /gene="rpoH"
                     /locus_tag="VG274_08915"
     CDS             complement(438..1334)
                     /gene="rpoH"
                     /locus_tag="VG274_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HWE06815.1"
                     /translation="MSSRGVPALQGEGGLSRYLSEIRKFPLLEPEEEYMFAKRWREHD
                     DPESARRLVTSHLRLVAKIAMGYRGYGLPISEIVAEGNVGLMQAVKRFEPDRGFRLAT
                     YAMWWIRAAIQEYILRSWSQVKMGTTAAQKKLFFNLRKVKSRIGALEEGDMRPEQVRE
                     IADKLAVPEEEVVNMNRRLSAPDSSLNAPLRSDSESEWQDWLVDETLDQESRLADAEE
                     MGERHDLLTTALGELTDRERDIIEARRLRDDPMTLEDLSQKYGVSRERVRQIEVRAFE
                     KLQQQMKRSLADKRRHTLAAAG"
     gene            complement(1474..>1661)
                     /locus_tag="VG274_08920"
     CDS             complement(1474..>1661)
                     /locus_tag="VG274_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06816.1"
                     /translation="RAKTPEEARAFAAVAEFSRQALHARVLGFKHPTSQKQMRFESAW
                     PADLAGLIGCLRVLQPR"
BASE COUNT          281 a          552 c          514 g          314 t
ORIGIN      
        1 gagctccgca aagagaagcc aggcgctcgc aagcgtggcg tgtccgcaca gatcgacttc
       61 cgtcgtcggc gtgaaccagc gcaaatcgta gcgcccactc tcgcggcgca ccaggaaggc
      121 ggtctccgcc agattgttct cgcccgcgat ttgctgcagg agccccggct cgagccagtc
      181 gggaagcggc acaatggccg ccggattgcc ctcgaaaacc ttctccgcaa acgcatcgat
      241 ctgccaaagc ctgagcttca tggtgaaccc ggagctacgt cagcttcaac ttagtgcggc
      301 gatcgcgcgc aacgtataac gcccggcgca tggcggccct gcgcaacgcc ctcgattttc
      361 ctcccccatc gtgggggagg aaagccgaat gcgcatcgcg tgcccctgga cagctgccgt
      421 gccgacggga gcgcgtttca gcctgctgca gccagcgtgt gccggcgctt gtcggcgagc
      481 gatcgcttca tctgctgttg cagcttctcg aaggcgcgca cttcgatctg ccgcacgcgc
      541 tcgcggctca cgccgtattt ctgcgacagg tcttcgagcg tcatggggtc gtcccgcagc
      601 ctgcgagcct cgatgatgtc gcgctcgcgg tcggtgagtt cgccgagcgc cgtggtcaaa
      661 agatcatgac gctcgcccat ttcttccgcg tcggccaggc gcgactcctg gtccagcgtc
      721 tcgtccacca gccagtcctg ccactccgat tcggagtcgg atctgagcgg cgcattgaga
      781 gacgaatcgg gggcagagag gcggcggttc atgttgacca cctcctcttc gggaacggcg
      841 agcttgtcgg cgatttcgcg cacctgttcg gggcgcatgt cgccttcctc gagcgcgccg
      901 atgcggcttt tcaccttgcg cagattgaag aagagcttct tttgggcggc ggtcgtgccc
      961 attttcacct ggctccatga gcgcaggatg tattcctgga tcgcggcgcg gatccaccac
     1021 atggcgtagg tggcaagccg gaaaccgcgg tcgggctcga agcgtttgac cgcctgcatg
     1081 agcccgacat tgccttcggc cacgatttcc gaaatcggca gcccatagcc ccggtagccc
     1141 atcgcgatct tggcgacgag gcgcagatgg ctggtgacga gacggcgcgc tgattccggg
     1201 tcgtcgtgct cgcgccagcg cttggcgaac atatactctt cttccggctc cagaagcggg
     1261 aacttccgga tttcagacag atagcgggag aggccgcctt cgccttgcag cgccggcacg
     1321 ccacgactgc tcatgttcaa agccccgtct ggcggcccgt acgcccaagc gcgcacgaca
     1381 ccgcctcatc cctctgggaa cgcccagggt ggttgttcgg tccgcggccg atatagccga
     1441 attttgccgc aaacttaaac tatgtaagtc gcttcaacgc ggctgcaaga cacgcaagca
     1501 gcctatcagc cctgccagat cggccggcca cgccgattcg aaccgcatct gcttctggct
     1561 cgtgggatgc ttgaatccga gcacacgcgc atggagggcc tgccgggaga attccgcgac
     1621 cgcggcgaag gcccgggcct cctctggcgt cttcgcgcgc g
//
LOCUS       DASZMC010000433         4538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5405066,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000433 DASZMC010000000
VERSION     DASZMC010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5405066"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /gene="rplE"
                     /locus_tag="VG274_08925"
     CDS             complement(<1..458)
                     /gene="rplE"
                     /locus_tag="VG274_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWE06817.1"
                     /translation="MVRPQLVEEFHYKNPLQAPRIKKVVLNIGAGEAAGDQKKIQSAV
                     NDLTAIAGQKAVVTRAKKAIATYKIREGLPIGVKVTLRKDRMYEFLDRLITIALPRVR
                     DFRGLNGRSFDGQGNYALGLKEHIVFVEIDYDKTENVWGMDIIINTSART"
     gene            complement(646..963)
                     /gene="rplX"
                     /locus_tag="VG274_08930"
     CDS             complement(646..963)
                     /gene="rplX"
                     /locus_tag="VG274_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWE06818.1"
                     /translation="MAAKIRRGDRVVVTTGRDRGKKGEVLKLYTKEARALVSGVNIVK
                     RHQRQTQKLQAGIINKESPVHLSNLAHVDPKTGDATRIGFKFLGDGRKVRFAKKSGEV
                     IDV"
     gene            complement(963..1331)
                     /gene="rplN"
                     /locus_tag="VG274_08935"
     CDS             complement(963..1331)
                     /gene="rplN"
                     /locus_tag="VG274_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWE06819.1"
                     /translation="MIQMSTELEVADNSGARRVMCIKVLGGSRRRYADVGDTIVVSVK
                     EAIPRGRVKKGEVLKAVIVRTAHGVRRPDGSLIRFDSNAAVLVNNQNEPIGTRIFGPV
                     TRELRARNHMKIISLAPEVL"
     gene            complement(1335..1595)
                     /gene="rpsQ"
                     /locus_tag="VG274_08940"
     CDS             complement(1335..1595)
                     /gene="rpsQ"
                     /locus_tag="VG274_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWE06820.1"
                     /translation="MPKRILQGVVVSDKNVKTVVVKVERRFTHPVMGKTVRRSKKYHA
                     HDENKEFKVGDVVRIQECRPLSRLKSWEVIGRVGAQERGTGA"
     gene            complement(1613..1828)
                     /gene="rpmC"
                     /locus_tag="VG274_08945"
     CDS             complement(1613..1828)
                     /gene="rpmC"
                     /locus_tag="VG274_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWE06821.1"
                     /translation="MAKKGTAAADLRVQTGDELGDSLLKLKKEQFNLRFQRANGQLEN
                     TSRMRVVRREIARMQTVLGEQHRKAGK"
     gene            complement(1834..2247)
                     /gene="rplP"
                     /locus_tag="VG274_08950"
     CDS             complement(1834..2247)
                     /gene="rplP"
                     /locus_tag="VG274_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWE06822.1"
                     /translation="MLQPARTKFRKQHKGRIHGAAKAGTSLNFGAYGLKATEPERITA
                     REIEAARRAITRHMKRAGRVWIRVFPDVPVSKKPAEVRMGSGKGAPEFWVARIKPGRI
                     LFEIDGVDTETAKEALRLGAAKLSIDTRFIARLEV"
     gene            complement(2260..2982)
                     /gene="rpsC"
                     /locus_tag="VG274_08955"
     CDS             complement(2260..2982)
                     /gene="rpsC"
                     /locus_tag="VG274_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWE06823.1"
                     /translation="MGQKVNPTGLRLGINRTWDSRWYAGKKDYGRLLQEDIKIRQHLS
                     ERLKQAGVSRIVIERPHKKCRITIHSARPGVVIGKKGADIEKLKNDVQRFTGDEVHLN
                     IVEIRKPEIDAKLVAENIAQQLERRVAFRRVMKRAVQSAMRLGAQGIRINCGGRLGGA
                     EIARMEWYREGRVPLHTLRADVDYGVATAKTTYGTCGVKVWIFKGEIMEHDPMATERR
                     QAEASDPNRAAGSGRAHPTPAH"
     gene            complement(2982..3416)
                     /gene="rplV"
                     /locus_tag="VG274_08960"
     CDS             complement(2982..3416)
                     /gene="rplV"
                     /locus_tag="VG274_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWE06824.1"
                     /translation="MGKKSRARVLPDTKALAIGRTIRTSPRKLNLVAQSIRGKTAEQA
                     LNELTFSPKRVAKDVKKVLQSAIANAENNHDLDVDELFVSEASVGKNLVLKRFHARAR
                     GRGARVEKPFAQVTIIVEQAKREEPAQKRAARNKTTAAKETA"
     gene            complement(3417..3698)
                     /gene="rpsS"
                     /locus_tag="VG274_08965"
     CDS             complement(3417..3698)
                     /gene="rpsS"
                     /locus_tag="VG274_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWE06825.1"
                     /translation="MARSVWKGPFVDGYLLKKAEAARGSGRKDVIKTWSRRSTILPQF
                     VGLTFGVHNGHKHIPVLVTEDMVGHKLGEFAPTRTFYGHSADRKAKRTA"
     gene            complement(3704..>4538)
                     /gene="rplB"
                     /locus_tag="VG274_08970"
     CDS             complement(3704..>4538)
                     /gene="rplB"
                     /locus_tag="VG274_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWE06826.1"
                     /translation="LKQYKPTTPGQRQLVLVDRSQLHKGGPVKGLTEGQTRHGGRNNT
                     GRVTVRWQGGGHKQRYRLIDFKRRKFDVPGRIERLEFDPNRTSFIALVKYKDGELAYI
                     LAPQRLAVGDMVISGDRVDVKPGNAMRLAAMPVGTIVHNVEMKPAKGGQIARSAGTYA
                     QYLGRDAGYAMLRLNSGEVRRVQLECMATVGAVSNPDHMNEVIGKAGRNVWKGKRPNV
                     RGTAMNPVDHPHGGGEGRTKGGRHPVTPWGKSTKGARTRKNKRTTKFIMRSRHAKRTQ
                     G"
BASE COUNT          704 a         1439 c         1319 g         1076 t
ORIGIN      
        1 tccgtccttg cggaggtatt gatgatgatg tccatgcccc agacattctc cgtcttgtcg
       61 taatcgattt cgacgaagac gatgtgctcc ttgaggccca gcgcgtaatt tccctgcccg
      121 tcaaagctgc gtccattcag accgcgaaaa tcccgcaccc gcggcaaagc aatcgtgatc
      181 aggcgatcca gaaattcata catccggtct ttgcgcaacg tgaccttgac gccgatcggg
      241 agaccctccc gaatcttgta tgtcgcaatc gctttctttg cgcgggtcac cacggctttc
      301 tgtccggcga tcgcggtgag gtcgttgacg gcggactgaa tttttttctg gtctccggcc
      361 gcttcgcccg ctccgatatt caggacgact ttctttatgc gcggcgcctg caaaggattc
      421 ttgtagtgga actcctcgac aagctgcggc cgaaccactt cgtcgtaccg cttcctgaac
      481 cgaggtacgt agtccgggtc gcgcggcgct tcgggcgctc gggcaggcgc ggcgtccgcc
      541 tttgcagccg gtttggattt cgccttccca tcgcgctgcc tggcatcgtc cttgccgcct
      601 tgggcctggg cctgcttttt tgcgcgcgtc tctttggcgc ctttttcaga catcgatcac
      661 ctcgccggat tttttcgcaa agcgcacctt gcggccgtcg ccgaggaact tgaatccgat
      721 ccgcgtcgcg tcgccggtct tgggatcgac gtgagcaagg ttcgaaagat ggaccgggga
      781 ttccttgttg atgatgccgg cctgtagttt ttgtgtttgg cgttgatgcc gcttgacgat
      841 gttcacccca gataccagag cacgggcttc cttcgtgtaa agcttcagta cctcgccctt
      901 tttgccccta tcgcgacctg tggtcacaac gacccgatcg ccccttcgga ttttggcagc
      961 catcagagca cctccggtgc gagcgagatg atcttcatat ggttgcgggc gcgaagttcg
     1021 cgggtcacgg gaccgaatat gcgtgtccct atgggttcgt tctggttgtt gaccaggacc
     1081 gcggcattgg aatcgaagcg gatcaagctg ccatcgggcc tgcgcacgcc atgcgcagtg
     1141 cgaacgatga cggccttgag gacctcgccc ttcttgaccc ggccgcgagg aatagcttcc
     1201 ttcacgctca ccacgatggt gtcgccgaca tccgcgtatc tgcggcgcga accgcccagc
     1261 accttgatgc acatgacccg gcgtgcgccc gagttgtcgg caacttcgag ttcggtggac
     1321 atctgaatca tggatcaggc tcccgtaccg cgttcctggg cgcccacgcg cccgatcact
     1381 tcccatgatt tgaggcgcga aagcggacgg cactcctgga tgcgcacaac gtcgcccacc
     1441 ttgaattcct tgttctcgtc atgcgcatgg tacttcttcg atcggcgcac cgtttttccc
     1501 atcaccggat gggtgaaacg gcgctcgact ttcaccacca ccgtcttgac atttttgtcg
     1561 ctgacgacga cgccctgcag gatgcgcttc ggcatttctg gttcctcgcc ctttatttgc
     1621 cggccttgcg atgctgctcg ccaagcactg tctgcattct tgcgatctcg cggcgcacca
     1681 cgcgcatgcg actcgtattc tcgagctggc cattcgcgcg ctggaagcgc aggttgaact
     1741 gctctttctt gagcttcagg agtgaatcgc cgagttcgtc tcccgtctgg acgcgaaggt
     1801 cggctgccgc tgtgcctttt tttgccattt cgttcagacc tcgagccgcg caataaacct
     1861 tgtatcgatc gaaagtttgg cggcccccag ccgaagggct tccttggcag tttcggtatc
     1921 gacaccatcg atctcgaaca ggatgcgtcc cggtttgatc cgggctaccc agaactcggg
     1981 cgcgcccttg ccggatccca tgcggacctc ggctggcttt ttcgaaaccg gcacgtccgg
     2041 gaatacccgg atccacacgc gtcccgcgcg tttcatgtgg cgcgtgatgg cgcgccgcgc
     2101 ggcttcgatc tccctggctg taatccgttc tggttccgtc gcctttagtc cataggcgcc
     2161 aaaattgagc gacgtgccag ccttcgccgc gccatgaatg cggcccttgt gctgcttgcg
     2221 gaatttcgtg cgcgcgggtt ggagcatggc ggctgtccgt cagtgagctg gagtcggatg
     2281 ggcgcgtccg gagccggcgg cgcggttcgg atcggaggct tcggcctgac gcctttccgt
     2341 ggccatcgga tcgtgttcca tgatctcgcc cttgaagatc cagaccttga cgccgcaggt
     2401 gccataggtc gtcttcgccg tggcgacacc gtaatccacg tccgcgcgca gcgtgtgcag
     2461 cggcacgcga ccctcgcgat accattccat gcgggcgatt tcggcgccgc cgagccggcc
     2521 accgcaattg atgcgtattc cctgggcgcc gagccgcatc gccgattgca ccgcgcgttt
     2581 catcacccga cgaaacgcca cgcgccgctc cagttgctgg gcgatgtttt ccgcgaccag
     2641 cttcgcatcg atttcaggct ttctgatctc gacgatgttg agatgaactt cgtctcccgt
     2701 gaatctctgc acgtcgttct tcagcttttc gatgtcggcg ccctttttgc cgatcacgac
     2761 tcccggccgg gcggagtgaa tcgtaatgcg gcacttcttg tgtggccgct cgatcacgat
     2821 gcgcgatacg cccgcctgct tgagccgttc gctcaaatgc tggcggatct tgatgtcttc
     2881 ctgcagcagg cgtccatagt ccttcttgcc cgcgtaccac cgcgagtccc aggtgcggtt
     2941 gatgcccaga cgcaagcccg tcggattgac cttctggccc atcaggccgt ctcctttgcc
     3001 gctgtggttt tgttgcgcgc cgcgcgcttc tgcgccggtt cctcgcgctt tgcctgctcg
     3061 acgatgatgg tgacctgcgc gaagggcttt tcgacgcgcg cgccgcgacc gcgtgctcgc
     3121 gcgtggaatc gcttgagcac aaggttcttt ccgacgctgg cctcgctcac gaaaagttcg
     3181 tcaacatcga gatcgtgatt gttttcggca tttgcaattg ccgactgaag aaccttcttc
     3241 acatctttgg cgacgcgctt gggcgaaaac gtcaactcgt tgagagcctg ttcggctgtc
     3301 ttgccgcgga tggattgcgc aaccaggttg agctttcgcg gactcgtgcg gatcgtgcgg
     3361 ccgatcgcca gggccttggt gtccggaaga acacgcgcgc gtgatttttt tcccatctat
     3421 gccgtcctct tggcctttct gtcggccgaa tgtccgtaga acgtgcgcgt cggcgcgaat
     3481 tcgccgagct tgtgcccaac catgtcctcg gtcaccagca cgggaatatg cttgtggcca
     3541 ttgtgcaccc caaaggtcag cccgacgaat tgcggcagga tcgtcgaacg ccgtgaccat
     3601 gtcttgatca cgtccttgcg tcccgagccg cgtgcagctt ctgccttctt gagcagatag
     3661 ccgtcgacaa acggaccttt ccaaacagag cgtgccatct cgattaccct tgcgttcgct
     3721 tcgcgtggcg cgaccgcatg atgaatttgg tcgtgcgctt gttcttgcgc gttctggcgc
     3781 ccttggtcga ttttccccac ggggtcacgg gatggcggcc gcccttggtc cgaccctcgc
     3841 cgccgccatg gggatgatcg accgggttca tcgccgttcc gcgcacgttc ggccgcttgc
     3901 ccttccagac gttgcggcca gccttgccga tgacctcgtt catgtgatcc ggattggaga
     3961 ccgcgccgac cgtcgccatg cattcgagct gaacgcggcg cacttctccc gaattgagac
     4021 ggagcatggc atagccggcg tcgcgcccaa gatattgcgc gtaggttcct gcggagcgcg
     4081 cgatctgccc gcccttggcc ggtttcatct cgacgttatg aacaatcgtg ccgaccggca
     4141 tcgccgcaag ccgcatcgcg ttgccgggct ttacgtcgac gcggtcgcct gaaatcacca
     4201 tgtcgccgac tgcgagacgt tgtggcgcaa gaatgtaggc cagctctcca tccttgtact
     4261 tcacgagcgc gatgaacgaa gtgcgattag ggtcgaattc gaggcgctcg atccggcctg
     4321 gaacgtcgaa cttccggcgc ttgaaatcga tgaggcgata acgctgcttg tggccgcctc
     4381 cctgccagcg caccgtcacg cggccggtgt tgttgcggcc accgtgtcgt gtctgtcctt
     4441 cggtaagacc cttgacggga ccgcccttgt gaagttgcga ccggtcgacg agcaccaatt
     4501 ggcgctgccc gggcgttgtc ggcttgtatt gcttgagc
//
LOCUS       DASZMC010000434         5894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4853730,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000434 DASZMC010000000
VERSION     DASZMC010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4853730"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VG274_08975"
     CDS             <1..345
                     /locus_tag="VG274_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HWE06827.1"
                     /translation="KQFADSQVAGGAQAANNQQVGTAVLGTILGAGLGAAIGGGRGAA
                     IGAASGAVVGTSVGANTSAYAQMSLQQRYDMAYAQCMNTKGDQVPGARHPRRYPPPGY
                     YPPPPGYPPPPG"
     gene            complement(548..2467)
                     /locus_tag="VG274_08980"
     CDS             complement(548..2467)
                     /locus_tag="VG274_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWE06828.1"
                     /translation="MGTPQDNVDQGRPWERSATRTAILAAARRVAERDGVLDMSLTNV
                     ANEAGFAPTSVYAYFTSKNDLLLAVISDDLATLARAMRGTAGDHDDESIGGAMRGAAP
                     MLLLPNALAAAGQTEQSLARPQTIPADPPKTAIAPADVSQPDAGLLPEHAEEQARRMP
                     VAERRDQIQTPSTAAPAGTAEAIARLQETVARLETRPVDAWLERRLREFERGLAALEA
                     RHGERAGGEASIDDRLGVLRQSLEHLESRLLSAAEDSARNLNQRLDASETRLLEIVSD
                     MHADGSQLAKRVTALESTSFTSQLPPFGSPDDPPPLQDLPTTPESQVHPQDAAPDEPV
                     RAAPEMSAPSYLAEARRSAQAAAVPRDEPMRRIPRKQSRMAFHAAAGSLVLFIALLAG
                     AGWLLRDQAMNVETTGAPARLVQPHPAHRGSTPVASIIVQNDPQARLRKWAEAGNTGA
                     ELLIGLEYLNGSGVARNDAGAYQWLARAAAKGQPLAQYNLGMLCENGRGVHADAVQAF
                     QWYGSAALRGNRRAMHSLAVAYADGLGTTKNLPEAAHWFERAATLGAVNSQFNLAVLY
                     ERGLGVKQSLGDAYKWYAIAAAQGDRESQARIAALTSSLPSSDLDGARNAAASFKPEA
                     VDATANFAPRPETLQ"
     gene            complement(2555..3388)
                     /locus_tag="VG274_08985"
     CDS             complement(2555..3388)
                     /locus_tag="VG274_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HWE06829.1"
                     /translation="MLASLLIVFREAIEAGLIVGIVLAATKGVAGRTIWVGCGVAGGV
                     AGAGLVAGFAGRIAASFQGSGQEIFDASILLLAVLMLTWHNVWMAGHGRAMARGMKQV
                     GAQVATGERPMAALAVVVGVAVLREGSEVVLFLYGIASQGGATATGMLAGGAAGLAAG
                     AATSALIYFGLLAIPAGRLFSVTSGLITLLAAGMAAQAILFIQQGGYFEYLTRTVWDT
                     SRILSQDSIIGRMLHTLVGYSDAPNGAQLLAYAATVLAIVALTRLERRRPHSGAVSRP
                     A"
     gene            complement(3393..3731)
                     /locus_tag="VG274_08990"
     CDS             complement(3393..3731)
                     /locus_tag="VG274_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWE06830.1"
                     /translation="MDPKIGLTCATLGWLLVAPSAMAGDSAPIELTLKDHKFAPAEIH
                     VKANVPSEIALTNADASAEEFDSSALKVEKVVAGGAKGIVRLRPLAPGRYPFMGEYHS
                     ETAQGVVIAE"
     gene            complement(3959..5257)
                     /locus_tag="VG274_08995"
     CDS             complement(3959..5257)
                     /locus_tag="VG274_08995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWE06831.1"
                     /translation="MKFGTFYEHQLPRPWIEGDELKLFQDALDQVELADRLGIDHAWE
                     VEHHFLEEYAHSSAPEVFLAACSQRTKQIRLGHGIVLMPPGYNHPARVAERIATLDLV
                     SRGRVEFGTGESSALLELGGFRVPVEKKREMWREGVEQCLNMLAMDPYPGFKGEFFEM
                     PCRNIVPKPVQRPHPPVWVACSNRETIKLAARLGIGALTFAFVDTAEAKHWVDDYYRI
                     FREECVPIGHAVNPNICMVTGFSVHHDGEEARRRGVEGFKFFGYALGHHYIFGEHKPG
                     RTNIWNRFEAAKDAMPPGAGAAGIGTPDEMRAHLRKFADAGVDQVAFIQQGGRNRHEH
                     ICEALDLFAREVMPEFKEPEAGRLARKREELAPFVEKAMARKQRLRELTDAEIPTVVA
                     LGRQIAATPDPQAEATARIWSQTAAVPLEDPLKAKAAAGK"
     gene            complement(5267..>5894)
                     /locus_tag="VG274_09000"
     CDS             complement(5267..>5894)
                     /locus_tag="VG274_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE06832.1"
                     /translation="NAGVAVGGPLDTISARDWDWIIDVNLKSAIYGVETFYPLIRSHG
                     EGGHMVNTASMAGMISPPGMEPYSATKFAVVAMSEGWAMQLQPLGIGVSVLCPGFVRT
                     RIHESGRARPERFGGHGEVNPGVGTTQREAAENVLHGIDPEIVGARVVEAVKAGELYI
                     FTHPAMREFVEARFQAIRSAFEAASGSLVLKSVKEWAPFTPTMPRPPI"
BASE COUNT         1060 a         1907 c         1841 g         1086 t
ORIGIN      
        1 aagcagttcg ccgacagcca ggtcgccggc ggcgcgcagg cggcgaacaa ccagcaggtc
       61 ggcaccgccg tcctcggcac gattctcggc gccgggctag gcgcggcgat cggcggcgga
      121 cgtggcgcgg cgatcggcgc ggcgtcgggc gcggtggtcg gcacatccgt cggagcaaac
      181 acgagcgcct atgcccagat gagtttgcag cagcgttacg acatggcgta cgcgcagtgc
      241 atgaatacga agggcgacca ggttccgggc gcgcgtcatc cgcggcgcta tccgccaccg
      301 ggctattatc cgcctcctcc gggctatccg cctcccccgg gctgaaccgg tgcttcagtt
      361 cagacaattg ggtcccaaaa aaattgtcat ggccccactt gatggggcca cccagccagc
      421 gcgcgtccgc gcgctgaata ttcaccttga gcgggtttcg actgggtggc cggctcggag
      481 tttaccctcc gaccgcgctc tcgcgcggat cggggtggcg ggccatgaca ttttttgggt
      541 gtgagcgcta ctgaagcgtc tcgggtctcg gagcaaagtt tgcagttgca tccacggctt
      601 cgggtttgaa cgacgcagcg gcattgcgcg cgccatcaag atcgctcgat ggtagcgacg
      661 aggtgagtgc agcaatgcga gcctgcgact cgcgatctcc ctgggcggca gcgatcgcgt
      721 accatttata ggcgtcgccg agactctgct tcacgccaag tccgcgctca taaagcaccg
      781 ccagattgaa ttgcgagttg acggccccaa gcgttgcggc gcgctcgaac cagtgcgcag
      841 cctccggcag atttttcgtg gttccaaggc cgtccgcata agcgactgcg aggctgtgca
      901 tcgcccggcg gttacctctc agggcggcgg acccgtacca ttgaaaggcc tgcaccgcat
      961 ctgcgtggac gccacggccg ttttcgcaaa gcattccgag attgtactgt gcaagcggct
     1021 gcccctttgc ggcggcacgc gccagccact gataggcgcc ggcgtcgttt ctggcgactc
     1081 cagatccatt caaatattca aggccgatga gaagttcggc tccggtgttg cccgcctcgg
     1141 cccattttcg caaacgcgcc tgcggatcat tttgcacaat gatcgaggcg accggagtgg
     1201 aaccccgatg ggcggggtgt ggctgaacca acctggccgg tgcgccagtg gtttcgacat
     1261 tcatcgcctg gtcacgcagc aaccagccgg ctcccgcgag caacgcaata aaaagcacca
     1321 gggaacctgc cgccgcgtga aaggccatgc gcgactgctt ccgggggatt cgccgcatcg
     1381 gttcgtctcg cggtacggcc gcagcctgcg ccgagcgcct cgcctccgcg agataggacg
     1441 gagcggacat ttcgggcgca gccctgaccg gttcgtccgg agcggcatct tgcggatgca
     1501 cttgcgactc gggcgtcgtg ggcaaatcct gaagcggtgg aggatcgtcc ggcgatccga
     1561 atggcggcaa ttgggaagta aacgacgtgc tttccagtgc agtcacgcgc ttcgccagtt
     1621 gcgacccatc ggcgtgcatg tcggacacga tctcgagcag gcgcgtttcg gaagcatcga
     1681 gccgttggtt cagattgcgc gcgctgtctt cggccgccga caaaagacga ctctcgagat
     1741 gttcgagact ctggcgcaat actccaagac ggtcatcgat tgacgcttca ccgcccgcgc
     1801 gctcaccgtg ccgggcctcc agggcggcca gcccgcgttc gaactctcgc agtcgccgct
     1861 cgagccacgc atccaccggt cgtgtttcaa gtctcgccac ggtttcttgc aggcgtgcga
     1921 tcgcctctgc cgtgccggcc ggcgccgcgg tcgaaggggt ctggatttgg tcacggcgct
     1981 ctgccacggg catgcgtctc gcttgctcct ctgcgtgctc cggcaataat cccgcatctg
     2041 gctgcgaaac gtctgccggc gcgatcgccg tttttggggg atcggcggga attgtttgcg
     2101 gccgcgccaa tgattgttcg gtctggccgg cggcggccaa agcgtttggg agcagaagca
     2161 tcggcgccgc gccacgcatc gcccccccaa tcgattcgtc atcatgatcg ccggcagtac
     2221 cgcgcatcgc ccgcgcaagg gtcgccaaat cgtcggatat cacggccagg agaagatcgt
     2281 tcttgctggt gaagtaggcg tagacggagg tgggagcgaa cccggcttca ttcgccacgt
     2341 tcgtcagcga catatccagc acgccgtcgc gctcggccac gcgccgcgca gcagcgagga
     2401 tcgcggttcg cgtggccgac cgttcccacg gccggccttg gtcgacattg tcctgtggcg
     2461 tccccatgcc ctccatcgta ggggacactt ggttaacgtc ttctgcatgc ggacaggccg
     2521 cacacgccgg gacccaactt ttttaggaaa cccgctatgc cggccgcgaa acagcgccgg
     2581 aatgcggccg gcgccgctca agccgcgtaa gcgcgacgat cgccagcacg gttgcggcat
     2641 aagccagaag ctgtgccccg ttgggagcat cgctatagcc gacgagtgtg tggagcatcc
     2701 ggccgatgat gctgtcctgg ctcaggatgc gcgacgtgtc ccaaacggtg cgcgtcagat
     2761 attcgaaata gccgccctgc tgaatgaaga ggatggcttg ggccgccatt ccagccgcca
     2821 acagagtgat caatcccgac gtaacgctga agaggcggcc ggcagggatt gcgaggagcc
     2881 caaaataaat gagtgcactg gtggcggcgc ctgccgccaa ccccgcggcc ccaccggcca
     2941 acatgcctgt tgcggtggcg cccccctggc tcgcgatgcc ataaaggaac agaacgactt
     3001 ccgagccctc gcgaaggacc gccacgccca caaccacagc aagcgccgcc atggggcgct
     3061 ctcccgttgc cacctgcgcg ccgacctgct tcatgccccg cgccatggcg cggccatgac
     3121 cggccatcca cacattgtgc caggtcagca tgagcacggc gagcaacagg atgctcgcat
     3181 cgaatatttc ctgtcccgaa ccctgaaagg acgccgcaat cctgccggca aagccggcaa
     3241 ccagcccggc tcctgcaacg ccgccggcga cgccgcatcc aacccatatc gtccggccgg
     3301 caacgccctt tgtggcagcc agcacgatgc cgacgatcaa gccggcctcg atggcttcac
     3361 gaaaaacgat gagaagactt gcgagcatac ggctattccg cgatcacgac cccttgtgcg
     3421 gtttcggagt gatattcgcc catgaaagga tagcggccgg gcgcgagcgg acgcagacgc
     3481 acgattccct tcgcaccccc agctacgact ttctcgactt tgagcgccga ggaatcgaat
     3541 tcttcggcgc tggcatccgc attggtcaaa gcgatttccg aaggcacgtt ggccttcacg
     3601 tgaatctcgg ccggcgcgaa cttgtgatcc ttgagggtca gttcgattgg ggcgctgtcg
     3661 cccgccatgg cggagggagc gaccaaaagc cacccgagcg ttgcgcaggt gagcccaatt
     3721 tttgggtcca tggggcgaaa tcccggaaat gcaccccagt ctacacgcga ctcattctca
     3781 ttcacaagag ggtaacgggt gcggcgaaag ggtgggcgag cggcgaatga gaccgttggt
     3841 ccccacgccc caacctcgtc ttggccaggc caaagcgaat atattggagc gatggctttt
     3901 tacgtggaca gctttggtga ttcatcagtt gcgcaagcga gtccccattc ggcacgtcct
     3961 actttccagc cgcggccttg gccttcaaag gatcttcaag cggcacggcc gcggtttgtg
     4021 accagatacg ggcggtcgcc tcggcctgcg gatcgggcgt cgctgcgatc tggcggccga
     4081 gggcgacgac ggtcgggatt tcagcgtccg tcaactcgcg caaccgctgc tttctcgcca
     4141 tcgccttttc tacgaaaggc gcaagctctt cacgcttgcg cgccagccgc ccggcctcgg
     4201 gttccttgaa ttccggcatc acttcgcgcg cgaaaagatc gagcgcttcg cagatgtgct
     4261 cgtgcctgtt gcgtccgccc tgctgaatga aagcgacctg gtccactcct gcatctgcaa
     4321 atttacgcag atgcgcgcgc atctcatcgg gagtgccgat gccggccgcg cccgcaccag
     4381 gcggcatcgc gtctttcgcc gcctcgaacc tgttccagat gttggtgcgc cccggcttgt
     4441 gctcgccgaa gatgtaatga tggccgagcg cgtaaccgaa gaacttgaat ccttcgacgc
     4501 cgcgccgccg cgcttcctcg ccgtcatgat gcaccgagaa ccccgtcacc atgcagatat
     4561 tcggattgac ggcgtggcca atgggcacgc attcctcacg gaagatgcgg tagtagtcgt
     4621 cgacccaatg ctttgcctcc gccgtatcga cgaaagcgaa ggtcagcgcg ccgatgccga
     4681 gccgtgccgc aagcttgatc gtctcgcggt tcgagcaggc gacccagacg ggcggatgcg
     4741 gccgctgcac gggcttgggc acgatgttgc ggcatggcat ttcgaaaaat tcgcccttga
     4801 acccgggata gggatccatg gcgagcatgt tcagacactg ctcgacgcct tcgcgccaca
     4861 tctcgcgctt cttctcgacg ggcacgcgga aaccgccgag ttcgagcagc gccgaggatt
     4921 cgccggtgcc gaattcgacg cggccgcgcg acaccaggtc aagcgtggca atacgttcgg
     4981 cgacgcgcgc cgggtggttg tagccgggcg gcatgagcac gattccgtgg ccgagccgga
     5041 tttgtttcgt gcgttgcgag caggcggcaa gaaacacttc cggcgcggag gaatgtgcat
     5101 actcctcgag aaagtgatgt tcgacctccc aggcgtggtc gatgcccagc cggtcggcga
     5161 gctcgacttg gtcgagcgca tcctgaaaaa gcttcagctc gtcgccctcg atccagggcc
     5221 gcggcaattg atgctcatag aaggtgccga atttcatcgc gcgctcctat attggcggcc
     5281 gcggcatcgt cggcgtgaag ggcgcccact ccttcaccga tttcaggacc aggctgccgg
     5341 acgcggcttc gaacgcggag cggatggcct ggaaacgcgc ctccacaaat tcgcgcatcg
     5401 cgggatgggt gaagatgtac agttctccgg ccttcaccgc ctctacgacg cgcgcgccca
     5461 caatttcggg atcaatcccg tgcaagacat tctctgccgc ttcgcgctgg gtggtgccga
     5521 caccgggatt gacttcgccg tgccctccaa agcgctccgg ccgagcacgg ccgctctcgt
     5581 gaattcgcgt gcgcacgaag cccggacaga ggaccgaaac cccgatgccg agcggctgca
     5641 attgcatcgc ccagccttca ctcatcgcga cgacggcgaa tttcgttgct gagtagggct
     5701 ccattcccgg cgggctgatc atgcctgcca tggaggccgt gttgaccata tgtccgcctt
     5761 cgccgtgact gcggatcagc ggatagaagg tttcgacccc gtagatcgcg cttttgaggt
     5821 tgacgtcgat gatccagtcc cagtcgcgcg cacttatggt gtccagtggc ccgccgactg
     5881 cgacgccggc gttg
//
LOCUS       DASZMC010000435         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4318607,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000435 DASZMC010000000
VERSION     DASZMC010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4318607"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VG274_09005"
     CDS             complement(<1..486)
                     /locus_tag="VG274_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06833.1"
                     /translation="MHDIRAIRENPAQFAAGIARRGMADAQAITGDLLARDRDLRLLQ
                     VRLQEAQAKRNEASRQIGAAKAQKDETRAAALMAEVAGLREQIQRDEDSERLQKGALD
                     EIISALPNLPASDVPDGVDETQNVEVITRRVGAAPTLNNAREHFELGEALGQMDFARA
                     AK"
     gene            complement(559..1374)
                     /gene="tatC"
                     /locus_tag="VG274_09010"
     CDS             complement(559..1374)
                     /gene="tatC"
                     /locus_tag="VG274_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWE06834.1"
                     /translation="MTARSEADEAAIEATKAPLLDHLIELRKRLVISIIAFIICFVLC
                     FAFAKYIYAFLTAPLAHALAGQPNHHLIYTALYETFFTYVKLGMFAGLCLSFPIIASQ
                     LWLFIAPGLYRHERNAFLPFLAATPVLFAIGAAFVYYVMLPFAIRFFVSYETPGGHGT
                     LGIELQAKVSEYLDFVTTLIFAFGLCFQLPVLLSLLGRVGIVSSAQLRSVRRYAILGI
                     VALSAVVTPPDVFSMISLALPLVFLYEVSIWCVRLIENRRAKVDAARAAGSAP"
     gene            complement(1371..1745)
                     /gene="tatB"
                     /locus_tag="VG274_09015"
     CDS             complement(1371..1745)
                     /gene="tatB"
                     /locus_tag="VG274_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HWE06835.1"
                     /translation="MIDLSWSHILLVIIVALVVVGPKDLPRLMRIAGQWLARARSMAD
                     QFRKSFDEMARQSELDELRAELDALRHERPLADLDHALNAPVLPPPGPAPAEASAAPA
                     APDAHEDVADAAERATATGPHQ"
     gene            1914..3437
                     /locus_tag="VG274_09020"
     CDS             1914..3437
                     /locus_tag="VG274_09020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWE06836.1"
                     /translation="MAYVALGLCILLASCAKPPPPPLPPPPAEVWPETSWTFSTPEAQ
                     GIDSEALAEAIEAIRNDNVPVHSLFVERNGYAVLDVYFFPFADNETHDLASVTKSVTS
                     TLIGIAQGQHRLGRLDQPIGSILPVAQTAAADPRKQRITVANLLSMTSGLDCRGNPAE
                     NVLLEMEQSPHWADFMLARVQAFDPGTTFEYCGGSMHVVSAVLSRSVGESAFDLAHQF
                     LFAPLGIAQAVWPVDPDGVSYGFAGLELQPRDAAKLGHLWLHRGLWKDQQLVPADYMV
                     AALTPRASVQPGVQYGYGMWLYPSHAPYDFQANGRGGQRITVVPEQDLVEVATAGGVD
                     ENRIAPLLAEAVRANRPLPPNPVGTAWLAKIVAWAALPPVAYAPAAAPQWAPTIAGKT
                     WLVGANPLGTHSLSLAFGSSAEGVIRFGFANGVVEDHPFGLDGVPRISVNTASGHRVA
                     LAGRWSDNAFDLDYDEIARINAYRLHFVLADNGLSIRITERSGLADLTLTATPAAAN"
     gene            complement(3675..>4120)
                     /locus_tag="VG274_09025"
     CDS             complement(3675..>4120)
                     /locus_tag="VG274_09025"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWE06837.1"
                     /translation="KPAILPAAAFFDDRGNRLSLAGFRGRPVLLNLWATWCAPCVREL
                     PALAHLQRAFPGLTIVAVNEGRETAADTTRFLKAHGADALHVYLDPDHAFLEALGVQG
                     LPLSVLIDARGMERAKASGPAEWDDPAAVAWLREVTAPADNRNAP"
BASE COUNT          735 a         1333 c         1341 g          711 t
ORIGIN      
        1 tttcgcagct cgcgcaaaat ccatctgccc cagcgcctcg ccgagctcga aatgctcgcg
       61 cgcattgttc agcgtcggcg cggcgccaac acggcgcgtg atcacttcga cattctgcgt
      121 ctcgtcaacg ccatcgggca catcggatgc gggaagattg ggaagggctg aaatgatctc
      181 gtcgagtgcg cccttctgca gccgctcgga atcctcatct cgttgaatct gctcgcggag
      241 ccccgccact tccgccatca gcgccgcggc gcgcgtctcg tccttctgcg cctttgcggc
      301 gccgatctgg cgcgaggctt cgttgcgttt ggcctgcgcc tcctggagcc gcacttgaag
      361 caggcgcaaa tcgcgatcgc gtgccaggag atcgccagtg atcgcctgcg cgtccgccat
      421 gccacggcgc gcgatacccg ccgcgaattg cgccggattc tccctgatgg cccggatgtc
      481 gtgcatgtgc ctccgcgggc cgcccccggc cgccagaccg gtaatggcat ggccgattcg
      541 acgggtaaag cggagacttt acggcgccga tccggcggcg cgcgcggcgt ctacttttgc
      601 gcgccggttc tcgatcaggc gcacgcacca gatcgaaacc tcgtagagaa aaacgagcgg
      661 gagggcgagg ctgatcatgc tgaagacgtc gggcggcgtc accacggcgg agagcgcgac
      721 gatgcccaga attgcgtagc ggcgcacact gcgcagttgc gcgctcgaga cgataccgac
      781 gcggccgagc agtgacagga gaaccggcag ctggaagcac aggccgaacg caaagatgag
      841 cgtcgtcacg aaatcgagat attcgctgac cttcgcctgc agctcgatgc ccagcgtgcc
      901 atgcccgccc ggcgtttcgt agctgacgaa gaatcggatc gcgaagggca gcatcacgta
      961 atagacgaag gctgcgccga tcgcgaacag cacgggcgtg gcggcgagaa agggcaggaa
     1021 cgcgttgcgt tcgtggcggt aaaggcccgg cgcgatgaac agccacaatt gcgaggcgat
     1081 gatcgggaac gagagacaca gccccgcgaa catgccgagc ttcacatagg tgaagaaggt
     1141 ctcgtagagc gcggtgtaga tcaggtggtg attgggctgt ccggccagcg catgggcgag
     1201 cggagcggtc aggaaagcgt agatgtactt cgcgaaggcg aagcagagaa cgaaacagat
     1261 gatgaaggcg atgatcgaaa tgacgagccg cttgcgcagc tcgatcaaat gatcgagcag
     1321 cggcgccttg gtggcttcga tggccgcctc gtcggcctcg ctgcgagcgg tcattggtgc
     1381 ggtccggtcg ccgtggcgcg ctctgccgca tcggcgacat cttcgtgcgc gtccggcgct
     1441 gcaggtgctg cggatgcttc cgcaggcgcc ggaccgggcg gcggcagaac gggcgcattg
     1501 agcgcgtgat cgagatcggc gagcgggcgc tcatggcgca acgcgtcgag ctccgcgcgc
     1561 aattcgtcca gttcggattg acgcgccatc tcgtcgaaac tcttgcggaa ttgatcggcc
     1621 atcgatcgcg ccctagcgag ccactgtccc gcgatgcgca tcagccgcgg caaatccttc
     1681 ggccccacca cgaccagcgc gacgatgatc accagaagaa tatggctcca gctcaggtcg
     1741 atcatcggct tatccgcacc tccactccat tttcctcccc cccgtgtggt ggaggtggat
     1801 tgcggagcga agcggagcaa gacggagggg ggagtcggca cgtgccgtgc gcccacgccc
     1861 ccctccgcct cgcttcgcgc ggcacctccc ctacacatgg gggaggaaaa tggatggcgt
     1921 acgtagcgct cggcctttgc attctgctcg cttcctgcgc gaagccgccg ccgcctcctc
     1981 tgcccccgcc acccgcggag gtgtggcctg aaaccagctg gacgttttcg actccggaag
     2041 cgcaggggat cgattcggaa gcgctggcgg aagcgatcga ggccattcgc aacgacaacg
     2101 ttcccgtcca cagcctgttc gtcgagcgca acggctacgc ggttctcgac gtctatttct
     2161 ttcccttcgc cgacaatgag acgcacgatc ttgcatcggt caccaagagc gtgacctcga
     2221 cgctcatcgg gatcgcgcag ggccagcaca ggctcggccg tctcgatcaa ccgatcggtt
     2281 caattcttcc cgtcgcgcaa accgccgctg ccgatccgcg caagcagcgc atcactgtgg
     2341 ccaatcttct cagcatgacc tccggcctcg attgccgcgg caatccggcg gagaacgtgc
     2401 tgcttgaaat ggagcagagt ccgcactggg cggacttcat gctggcgcgg gtgcaggctt
     2461 tcgaccccgg caccacgttc gaatattgcg gcggcagcat gcatgtggtg tccgccgtgc
     2521 tttccagatc agtcggcgaa agcgcgttcg atctcgcgca tcaatttctg ttcgcgccgc
     2581 tcggaatcgc ccaggccgtc tggcccgtgg atcccgacgg cgtgtcgtat ggttttgcgg
     2641 gactcgagtt gcagccgcgc gacgcggcga agctcggcca tctctggctg cacagagggc
     2701 tctggaagga tcaacaactc gttcccgccg attacatggt cgctgcgctc acgccgcgcg
     2761 ccagcgtgca gcccggcgtt caatatggct acggcatgtg gctctatccg tcgcatgcgc
     2821 cttacgattt ccaggcgaat ggccgcggcg gccagaggat caccgtcgtt ccggagcagg
     2881 atctggtcga ggttgcgacg gcgggcggcg tcgatgaaaa tcgcattgcg ccactcctcg
     2941 ccgaagcggt gagggccaat cgtccgctgc ctcccaatcc ggtcggcacg gcatggctgg
     3001 caaagatcgt ggcgtgggcg gctcttccgc ccgtggctta tgcgccggcg gccgctcccc
     3061 aatgggcgcc cacgatcgcc ggcaagactt ggctcgtcgg cgcaaatcca cttgggacgc
     3121 acagcctgtc gctcgcgttc gggtcatcgg cagaaggcgt gatccgtttc ggcttcgcga
     3181 acggggtcgt cgaagatcat cctttcgggc tcgatggcgt accgcgcatt tcggtgaaca
     3241 ccgcgtcggg ccatcgcgtc gcgctggccg gcaggtggag cgacaacgca ttcgatctcg
     3301 actatgacga gattgcccgc atcaacgcct accggctgca tttcgtactg gccgacaacg
     3361 gactaagcat ccgcatcacc gaacgcagcg gcctcgccga cctcacgctc accgccaccc
     3421 cggcggccgc gaactgagcc gcgcctgagc ggttgctccc cctcacctgc gcttgagcgg
     3481 tcgatggacc tcgttcttga gccctctccc gcacggggag aggggacccc gagcgctgat
     3541 tttcctacgt tggtgcaaga ctccgattcc cctctcccct cgtgggagag ggccgcaggt
     3601 acgtgcaaaa aattctgttc agacgcgggt gagggggagc aaccgctcaa gcgcaggtga
     3661 gcggtgggcg accgctacgg cgcgttccga ttgtctgctg gcgccgtcac ctcgcgcagc
     3721 caggcgacgg cggcgggatc gtcccactcc gcgggacccg acgctttcgc gcgctccatg
     3781 ccgcgcgcat cgatgagcac gctcaaggga agaccctgga caccgagcgc ttcgaggaat
     3841 gcgtgatcgg gatccagata cacgtgaagc gcgtccgcgc cgtgcgcttt cagaaagcgg
     3901 gtggtgtcgg cggctgtttc acgcccctca ttgaccgcaa cgatggtcag gccgggaaag
     3961 gcgcgttgca gatgcgccag ggccggcagt tcgcgcacgc aaggcgcgca ccaggtcgcc
     4021 cacagattga gaagaacagg acggccgcga aacccggcca gcgaaagacg gttgccgcga
     4081 tcgtcgaaga aggcggcggc cggaagaatt gcgggctttg
//
LOCUS       DASZMC010000436         5399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_752056,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000436 DASZMC010000000
VERSION     DASZMC010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752056"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VG274_09030"
     CDS             complement(<1..227)
                     /locus_tag="VG274_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWE06838.1"
                     /translation="MRRNMFWLNDEQWRQIEPHLPKDVRGKARVDDRRVISGIIHVLK
                     SGCRWCDRPPEYGPSTAAAQSQIGGWRLPQGR"
     gene            342..710
                     /locus_tag="VG274_09035"
     CDS             342..710
                     /locus_tag="VG274_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06839.1"
                     /translation="MVEATCHCGAVRVEVPKPPQEVEVKECNCSICRRTGAVLAYYSP
                     RDVRVSGATDIYVCGDREIEFHRCKICGNFTHWAPVHKSLDRMGVNVRLMPPELLTDL
                     RILKFDGADTWTVVGERRGI"
     gene            complement(774..1076)
                     /locus_tag="VG274_09040"
     CDS             complement(774..1076)
                     /locus_tag="VG274_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06840.1"
                     /translation="MDASIQKPRFTDKQGQYLAFIHAYTLVMGRPPAEADMQRFFAVT
                     PPSVHLMVLTLERLRLISREPGVARSIKLLVTPENLPQLQPTVSSVQRDWGQNPLI"
     gene            1812..3086
                     /gene="efeB"
                     /locus_tag="VG274_09045"
     CDS             1812..3086
                     /gene="efeB"
                     /locus_tag="VG274_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HWE06841.1"
                     /translation="MTRRDRTAGSRRGFLKRAGLLAAIAGTGLTQAEIRPAAAEAGSA
                     EAVEPFYGAHQGGILTPAQRHTYFTAFDLDTRKRADVIALLLMWTEAAALMSRGEMAP
                     SQGSDSDAPADIAETMGLSPSRLTITFGFGPGLFIKNGKDRYGLGASRPPALIDMPAF
                     TGDQLLADRTGGDISVQACADDPQVAFHAIRRLSRLGTGIAQIRWGQTGFLPHSPEGE
                     TPRNLMGFKDGTGNPSLQDGTAMEKFVFTGDDGPAWMRGGSYVVARRILIALEHWDRM
                     RTNFQEQTFGRHKLSGAPLGKNNESDTLDFEAADKDGNPRTPINAHVRLAAAQSNGGA
                     RVLRRPYSYNDGVSYTAERWPPWRQGIEYDAGLFFICYQRDPREGFVRIYERMSKLDL
                     LNQFSTHTGGGMFACPPGAAPGEYIGQKLFET"
     gene            3291..4667
                     /gene="trmFO"
                     /locus_tag="VG274_09050"
     CDS             3291..4667
                     /gene="trmFO"
                     /locus_tag="VG274_09050"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HWE06842.1"
                     /translation="MLDSEISAALHIIGGGLAGCEAAWQAARAGVAVVLHEMRPVRGT
                     EAHATDGLAELVCSNSFRSDDRENNAVGLLHEEMRRAASLILRAADLNRLPAGGALAV
                     DREGFSRAVTRAIEEHPRIDIRREEIDMFPDTPVIAATGPLTSPALAQSIRALTGEDS
                     LAFFDAIAPVVHKDSIDFSVAWLQSRYDKVGPGGGGADYINCPLDEDQYRAFVAALIA
                     GEKTSFKEWEESTPYFEGCLPIEVMAARGLETLRFGPMKPVGLRDPRKEHRPHAVVQL
                     RQDNALGTLWNMVGFQTKLKYGEQTQIFRMIPGLEQARFARLGGLHRNTFINSPRLLD
                     RQLRLRSCPRVRFAGQITGVEGYVESAAIGLLAGRFAAAEILGEDSAPPPATTALGAL
                     LGHITGGAAAETFQPMNVNFGLFPEIVDGSRGRDRKKALAHRALRDLDKWLGSVETKK
                     IHNEAVRQ"
     gene            complement(4742..>5399)
                     /locus_tag="VG274_09055"
     CDS             complement(4742..>5399)
                     /locus_tag="VG274_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HWE06843.1"
                     /translation="IRLQWWREALEGACDGRPRAHPASIGLAEIFACASPPRELFERL
                     VDARAPESASAPLADLEALEAYADASSASLMRIAAHILDMWADVEIAAREAGRAYGLA
                     RILRAIPRCVARGRMPLPADLLVAEGLSVADVFSARDSVALKRAIARVADAAQTHFAQ
                     ARKMQIPRETRAAFLPASLVPAYLSQIARSSNPLRDRHDISQLRRQLVLLRSAILGRL
                     "
BASE COUNT         1020 a         1695 c         1718 g          966 t
ORIGIN      
        1 cgcccctggg gcaatcgcca tccgccgatc tgggattgag cagcggcggt cgacgggccg
       61 tattccggcg gacggtcgca ccagcgacaa ccgcttttga gcacatggat gatgccgctg
      121 atcacgcggc ggtcatccac ccgcgccttg ccgcgcacat ccttcggcaa atgcggctcg
      181 atctgccgcc actgctcatc gttcagccaa aacatattgc gccgcatcgt ctccgctccg
      241 aatcaacaga gacagcgaat cagcaaccga ttcagccagc aatgatttta ctggattttc
      301 accctagagt gcttccattc acagcgcgag aggagccaaa gatggtcgag gcaacatgcc
      361 attgcggggc ggtgcgggtg gaagttccca agccgccgca ggaagtcgag gtcaaggagt
      421 gcaactgctc catctgccgg cggacgggcg cggtactggc gtactactcg cctcgggatg
      481 tgcgggtcag tggcgcgacc gacatatacg tgtgcggcga ccgcgagatc gaatttcacc
      541 gctgcaagat atgcggcaac ttcacccact gggcgccggt gcacaaatcg ctggatcgca
      601 tgggcgtgaa cgtgcgtctg atgccgcccg aactgttgac cgatctgcgc atcctgaagt
      661 tcgacggcgc ggatacctgg acggtcgtcg gggaacgacg aggtatctag ggtgtacttt
      721 cacgttccgg atttatcagg cccgcgaact gccgaatttg cgggcgacgt ggttcaaatt
      781 aatgggtttt ggccccagtc cctctgcaca gaggatacgg tgggttggag ctgaggcaaa
      841 ttttcaggtg tgacgagcag tttgatactt cgcgctacgc cgggctcgcg gctgatcaag
      901 cgcaatcgtt cgagggttag caccatcaga tggaccgaag gcggggtgac ggcgaagaag
      961 cgttgcatgt cggcttcggc gggtggacgg cccatcacga gagtataggc atgaatgaag
     1021 gccagatact ggccctgctt atcggtgaac ctcggttttt ggattgaagc gtccacgtca
     1081 tatagtttga ctccaccggg cctcgaatca caaggaggcc ctcgacgaat atccgttaca
     1141 gtgtggaact tacgcccgaa gagcgcgacg atttgtcaag ctgacgcatc agctgtcgcc
     1201 ggattctgcg ggccggccag cccaaagcaa tcgccctggc gcgggatgca aaacgttcca
     1261 agtacgcaga ggattgcgct gtgaaacgca cttgaccttg acgtgtttga tcaaacgccg
     1321 tatcgattgc ggcatggcga aaagttgcac ccggcgccgg agcggtggcc gggcttttta
     1381 ttggccggcg cgacgaaacc tcccggaccc gcggattgcg catcttcgct gttaaaatcg
     1441 gtgttcgacc cttcgtcatc gtgaaatgtt gcaccccgcg ccgaagcggt ggtccgggtt
     1501 tttattgacc cgcgcgacga aacctcccgg cgccacggat tgccgatctt cgctgttaaa
     1561 ggtgatcttc gctgttaaat tcgctgttcg gcccttgccg caaatggcgg ggcggcgatt
     1621 tgcccgctgc tttcgtggcg gcctgccgcc cgcagacact ggcgcaccgg acacaaattc
     1681 gaacccctga cgggttcagg tgcgcaatcg ccgcccggaa agggcgatct cttcggcctt
     1741 gtggtggcac caggcgggaa gggattctat tatgtcgagg acgatgtgaa cacgctcatg
     1801 gaagcctctc agtgacacgg cgggacagga ctgcaggttc gcggcgcggg tttctgaagc
     1861 gcgcagggct gctcgctgcc attgccggca cgggattgac gcaagccgag atccggccgg
     1921 ccgcagcgga agcgggatcc gcagaagctg tcgagccatt ctacggcgcg catcagggcg
     1981 gcatcctcac acccgcacag cgccatacct atttcaccgc gttcgatctc gatacccgga
     2041 agcgcgccga tgtgatcgcg cttctgctca tgtggacaga ggccgcagcc ctgatgtcga
     2101 gaggcgagat ggcgccttcg cagggaagcg attctgatgc gcccgccgac atcgccgaga
     2161 cgatgggcct ctctccgtcc cgtctaacga taaccttcgg gttcggtccc gggctcttca
     2221 tcaagaacgg caaggaccgt tatggcctcg gcgcttcacg tccgccggcg ctcatcgaca
     2281 tgccggcctt caccggcgac cagctcctgg cggaccgcac gggcggagat atttccgtgc
     2341 aggcctgcgc cgacgatccg caggtcgcct ttcacgccat ccggcgcctc tcgcgtctcg
     2401 gcaccggaat cgcgcagatc cgctggggcc agacgggatt cctgccgcat tcgcccgaag
     2461 gtgaaacgcc gcgcaacctg atgggattca aggacggcac cggcaatccc tccctgcagg
     2521 acgggacggc gatggaaaaa ttcgtcttca ccggcgatga cggtccggcc tggatgcgcg
     2581 gcggttccta cgtcgtcgcg cggcgcatcc tcatcgcgct cgagcactgg gaccggatga
     2641 ggaccaactt tcaggaacag accttcggac gccacaagct gagcggtgcg ccgctcggaa
     2701 aaaataacga gtcggacacg ctcgatttcg aggccgccga caaggacggc aatccccgca
     2761 cgccgatcaa cgcgcatgtg aggctcgccg cagcgcagag caatggcggg gcccgcgtgt
     2821 tgcgccggcc ttattcctac aatgacgggg tgagctacac ggcggaacgc tggccaccct
     2881 ggcggcaggg catcgaatat gacgcaggcc tgttctttat ctgctatcag cgcgacccgc
     2941 gcgagggctt tgtcaggatc tacgagcgta tgtccaaact cgacctgctc aaccagttct
     3001 cgacccacac cggtggcgga atgtttgcct gcccgcccgg cgccgcgccc ggcgaataca
     3061 tcggccagaa gctgttcgag acgtaggcag agcgcccacg cggagccgcg taggccgcga
     3121 agaagagaag ttcacgggga gatagtgagc cggtgaggaa gaatcgcgcg ctgcgcgcgg
     3181 cgcagcgggt tggcgcgcaa ctggtctcac tgtcttaccg tctgcccgtg actcttacgt
     3241 ctgcttgcaa atcctggttc gccgtaaccc tccaaatgct acattgagcc atgctcgata
     3301 gcgaaataag tgctgcgctt cacatcatcg gtggagggtt ggcggggtgc gaggcggcgt
     3361 ggcaggcggc acgggccggc gtcgccgtcg tgctgcacga gatgcggccg gtgcgcggaa
     3421 cagaagcgca tgcaacggat gggctcgccg aacttgtgtg ctcgaactcc ttccgctccg
     3481 acgatcgcga aaacaatgcc gtcggcctgc tgcatgagga aatgcggcgc gcagcttcgc
     3541 tcattctgcg cgcggccgat ctcaaccggc tgcccgccgg cggcgcgctt gcagtcgacc
     3601 gcgaaggatt ttcacgcgcc gtgacgcgtg cgatcgagga gcatccgcgc atcgatatcc
     3661 gtcgtgagga gatcgacatg ttccccgaca cgcccgtcat cgccgcgacg ggaccgctga
     3721 cttctccggc gctcgcccag tcgatccgcg ctctgaccgg cgaggattcg ctcgccttct
     3781 tcgacgccat cgcgccggtc gtccacaagg acagcatcga tttctccgtc gcctggctgc
     3841 agtcgcgcta cgacaaggtg ggaccgggcg gcggcggcgc cgattacatc aattgccctc
     3901 tcgacgagga tcagtaccgc gcattcgttg cggcgctgat cgcaggcgag aaaacaagct
     3961 tcaaggaatg ggaagaatcg acgccctatt tcgaaggctg tctgcccatc gaagtgatgg
     4021 cggcgcgcgg actggaaacg cttcgcttcg gcccgatgaa gcccgtgggc ctccgggatc
     4081 cgcgcaagga acatcggccg catgcggtcg tacaactgcg ccaggacaat gcgctcggca
     4141 cgttgtggaa catggtgggc ttccagacca agctcaagta tggcgaacag acgcaaattt
     4201 tccgcatgat cccgggcctc gaacaggcgc gcttcgcgcg gcttggcggc ctgcaccgga
     4261 atacgttcat caactcgccg cgcctgctcg accggcaatt gcgcctcaga tcgtgtccgc
     4321 gcgtccgttt cgcggggcag atcaccggcg tcgaaggcta tgtcgagagc gcggcaatcg
     4381 ggctgctcgc cggccgcttc gccgccgcgg aaattctcgg cgaggattcc gctccgcctc
     4441 cggcgacgac cgcgctgggc gcgctgctcg gccacatcac gggcggcgcc gcagccgaga
     4501 cgtttcagcc catgaacgtg aacttcgggc tgttccccga aatcgtagac ggaagccgcg
     4561 gccgcgaccg gaaaaaagcc ctcgcccacc gcgccttgcg cgatctcgac aaatggctcg
     4621 gaagtgtcga gacgaaaaag attcacaatg aggcagtgag acagtgaggt gttccccgcg
     4681 cttcgcgcgc tgtctttcct caccgcctca ctgcctcatt gtgaattctt gctttcgcaa
     4741 ctcacaggcg tccaaggatt gcggagcgga gcagcaccag ctggcggcgg agttgggaga
     4801 tgtcgtgccg atcgcgcagc ggattgctgc tgcgcgcgat ttgcgacaga taggccggga
     4861 ccagcgaggc gggcaggaac gctgcacggg tctcgcgcgg aatctgcatt tttcgagcct
     4921 gggcaaaatg cgtctgcgcc gcgtcggcga cgcgtgcgat cgcgcgcttc aacgctacgg
     4981 aatcgcgcgc cgagaacacg tcggcgacag acaggccttc cgcgacgagg agatcggcgg
     5041 gtagcggcat tcttccccgc gcgacgcatc gcggaattgc acgcagaatc cttgcgaggc
     5101 cgtaagccct ccccgcttcc cgggcggcaa tctccacatc ggcccacata tcgagtatgt
     5161 gcgccgcaat ccgcatcagc gaggcggagg acgcgtcggc ataagcctca agcgcttcca
     5221 gatcggccaa cggcgccgag gcggactccg gcgcgcgcgc atcgacgagg cgctcgaaca
     5281 attcgcgcgg cggcgaggcg caggcgaaga tttcggcaag cccgatggaa gcaggatgcg
     5341 cgcgcggacg gccgtcgcac gcgccctcca gcgcctcgcg ccaccattgc aggcgaatc
//
LOCUS       DASZMC010000437         4985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5128726,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000437 DASZMC010000000
VERSION     DASZMC010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5128726"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VG274_09060"
     CDS             complement(<1..735)
                     /locus_tag="VG274_09060"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HWE06844.1"
                     /translation="MSIPQSAGGPIDSRDDLVRYLEQGGKPRAQWRVGTEHEKFVYDL
                     ATRMPLTYAGRPGIRALLEGMRRFGWEPVMEGDRIIGLSQGAASISLEPGGQFELSGA
                     PLRSLHQTCAEVNTHQAQVREVAAEIGAGVLGLGFAPTWRLDDVDQMPKGRYAIMRRY
                     MPKVGGYGLEMMFRTCTVQVNLDFADEADMVKKFRVALALQPVATALFANSPFREGQP
                     NGFLSYRSQIWTDVDNARAGMLPFVFE"
     gene            911..1249
                     /locus_tag="VG274_09065"
     CDS             911..1249
                     /locus_tag="VG274_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06845.1"
                     /translation="MTSRIMPAVVCAALLCSAAPCWSMQTVTLPQPNDGSAPYQGVDN
                     PGQTNFSNDQGATTDNSLGHFHFSTSSDSSSPDDPGYYYRPGGSSRPGYGTSNVPGSE
                     FYNSGYPFPH"
     gene            complement(1358..2104)
                     /locus_tag="VG274_09070"
     CDS             complement(1358..2104)
                     /locus_tag="VG274_09070"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008694.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HWE06846.1"
                     /translation="MPDREVTFPGGKVRLFVEEALREDARAVLSAPQSHYLLHVMRAH
                     AGDRVRLFNGCDGEWLARIDEVGRRDCTLVCERRTAEQEADCDLWLVFAPIKKTPADY
                     LAQKATELGVSVLQPVYTRRTIVHRLNMERMRTNAIEAAEQSGRLTVPEIREPLDLAR
                     LLANWPRERQLLFCDETGGPPILEALSRASAGPWAVLTGPEGGFDESERTMIRAHAAT
                     VPASLGARILRADTAALAALAVWQALYGHQ"
     gene            2395..3324
                     /gene="ubiA"
                     /locus_tag="VG274_09075"
     CDS             2395..3324
                     /gene="ubiA"
                     /locus_tag="VG274_09075"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HWE06847.1"
                     /translation="MPSTLPADALPHSWVDAAPMAVQPYLRLMRLDRPIGAALLFLPC
                     VFGLATGATLQHRGFGIADDLALLALFGLGAIVMRGAGCTYNDIIDRDIDAKVARTRG
                     RPIPSGAVSVRAASIFLALQCAAGLVILLQLNAFSIALGAGSLVLIALYPFMKRVTWW
                     PQAWLGLTFNWGALLGFSAETGSLALPAWLLYAGCFFWTLGYDTIYAHQDAEDDALVG
                     VQSTARLFGERSGPWIAAFYACAIACIGAAMWFSMARTWSLFLLAPAGLHFLWQVRRL
                     DHGDTTRYLTIFRSNRDTGLLIGAALVAASYVH"
     gene            3472..3909
                     /locus_tag="VG274_09080"
     CDS             3472..3909
                     /locus_tag="VG274_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HWE06848.1"
                     /translation="MSQFVAPQSIVALVLYALWAIALVLMVGADRVLMVIRGKAKADS
                     FTPGVPHGGDSYWRINRAHVNTVENLPIFAAIVLAGWVVGMETATFNRLAIIVVVARI
                     VQSAIHIASGSVVAITFRFTALAIQLICEIWMAILVLRAGGLF"
     gene            3984..4769
                     /locus_tag="VG274_09085"
     CDS             3984..4769
                     /locus_tag="VG274_09085"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWE06849.1"
                     /translation="MRRSVENIGHHRYDRNMMQGHPAEFIRANTELAAPPLIPEIDLH
                     LATEVVPLWRKTEEELQREGVPPPYWAFAWAGGQALARYVLDHRETVATRAVLDLGTG
                     SGLVAISAAKAGARSVLAADVDEFAAAAIALNCWANGVSCVTRRPAAKRPAVARRGEK
                     IEITTDDVIGHRCAWDVILVGDMCYERPLAERLLQWLSQCAEGGTQVFLGDPGRAYFP
                     ESGTVRLATYRVPTSRELEDREIRETSVFRLGSMNGALGLGGA"
BASE COUNT          842 a         1630 c         1528 g          985 t
ORIGIN      
        1 ttcgaacacg aacggcagca tgccggcgcg cgcgttgtcg acgtcggtcc agatctggct
       61 gcggtaggaa agaaagccgt tcggctggcc ctcgcgaaaa ggcgaattcg cgaacagggc
      121 cgtggccacc ggctgcagcg caagcgccac ccggaatttt ttcaccatgt cggcttcgtc
      181 ggcgaagtcg agattgacct gcaccgtgca agtgcgaaac atcatttcga ggccgtagcc
      241 gccgaccttc ggcatgtagc gccgcatgat tgcgtatcgg cccttgggca tctgatccac
      301 gtcgtcgagc cgccaggtcg gcgcaaaccc gagccccaga acgccggcgc cgatttcggc
      361 cgcaacttcg cgcacctggg cctgatgcgt gttcacctcc gcgcaggtct gatgcagcga
      421 tctgagcggc gcgcccgaaa gttcgaactg tccgcccggc tccaggctga tcgaggccgc
      481 gccttgcgaa agaccgatga tgcggtcgcc ctccatcacc ggctcccagc cgaaacggcg
      541 catgccttcg aggagcgcgc gaatgcccgg cctgcccgcg taggtgagcg gcatgcgggt
      601 cgcgaggtcg tagacgaatt tctcgtgctc ggtgccgacg cgccattgcg cgcgcggctt
      661 gccgccctgc tccagatagc gcacgagatc gtcgcgcgag tctatcggtc cgcccgcgga
      721 ttgggggatc gacatcggcg gtcctcctgg ctttccccct cgaagggatc agcccgtgga
      781 agtcatcaag cgggctggcc gcgtatgtag gcgcggcgtc ccgccccggc aagcggggct
      841 cgcttgccgc cattgccaca aaggccgcaa acgtgatttg atttgccggt acggttcgag
      901 gagtgccgcc atgacatcga ggatcatgcc tgccgtcgtt tgcgcagccc ttctatgttc
      961 ggctgcgcca tgctggtcca tgcaaacagt gacattgccg cagcccaatg atggcagcgc
     1021 gccctatcaa ggcgtggaca atccggggca aaccaacttc tcgaacgatc aaggcgccac
     1081 gacggacaac agcctcggcc acttccattt cagcacgtcg tccgattcaa gctctcccga
     1141 cgacccgggc tattactacc ggccgggcgg ttcttcgcgc ccaggctacg gtacgtcgaa
     1201 cgtgccgggc tccgaattct acaattcggg ctatccattc ccgcactgag cctcttcgtg
     1261 ccaccggtcc gcgcgaagcg cggccggagc gtaaacttca gcgcggaccc gtctcccggt
     1321 gtccgcacag cgcttcccgc gaaagcgggg acccatccta ctggtgtccg tacagcgcct
     1381 gccacactgc aagagcagcc agtgccgccg tatcggcgcg gagaatgcgc gcgcccagtg
     1441 acgcgggcac cgttgcggca tgggcacgga tcattgtgcg ttcggactcg tcgaaaccac
     1501 cttccggccc ggtcagtacc gcccatggcc ccgctgaggc gcgtgaaagc gcttccagaa
     1561 tcggcggacc tcctgtctcg tcgcagaaga gcaactgccg ctctcgcggc caatttgcga
     1621 gcagccgggc gagatcgagc ggctcacgga tttccggcac ggtcagccgc cccgactgct
     1681 ccgcggcttc gattgcgttg gtccgcatgc gctccatgtt gaggcgatgc acaatggttc
     1741 gccgtgtgta gacaggctgc agcaccgata cgcccagctc ggtcgccttt tgcgcgaggt
     1801 aatccgccgg cgtcttcttg atcggcgcga agaccagcca gagatcgcaa tcggcctcct
     1861 gttccgcggt ccggcgctcg cagacaagtg tgcagtcgcg ccgccccact tcgtcgatcc
     1921 gcgccagcca ttcgccgtca cacccattga acagccgcac ccggtctccg gcatgcgcgc
     1981 gcatgacatg aaggaggtaa tgcgattgcg gcgcgcttaa aacagcgcgc gcgtcttcgc
     2041 gcaacgcctc ctccacgaac agccgcacct taccgccagg aaatgtaact tcgcgatccg
     2101 gcacccaccc caccctttcc aacccgattc gactttcctc ccccatgtgt gggggaggtg
     2161 cctcccccgg attaaatccg ggggaggcgg cggggcttgg gccgacacgg cgcgccgact
     2221 ctccctccgt cttgctccgc ttcgtggagt ttatcctccg gccgcgcttc gcgcggaccg
     2281 ggcggctatc caccccacac atggggagga aaattagccg cgtgcggggg aggaaagtaa
     2341 atcgctcgtg tcaatcaaat tcagccgtga cacattcaat ccatggaccc tgttgtgcca
     2401 tccaccctgc ccgccgacgc gttaccgcac agctgggtcg atgccgcgcc catggccgtg
     2461 cagccttatc tgcgtttgat gcggctcgac cggccgatcg gcgcggcgct tctcttcctg
     2521 ccctgcgtct tcggtcttgc tacgggcgca acgttgcaac atcgcggttt tggtatcgcg
     2581 gacgatctcg cgctgctagc gttgttcggc ctcggcgcca tcgtcatgcg cggcgccggg
     2641 tgcacctaca acgacattat cgatcgcgat atcgacgcga aagtcgcgcg cacgcgcggg
     2701 cgccccattc cttctggcgc ggtcagtgtt cgcgcagcct cgatatttct cgcgcttcaa
     2761 tgtgcggcgg ggctggtcat cctccttcaa ctcaatgcgt tctccatcgc gctcggtgcg
     2821 ggctcgcttg tccttattgc tctctatccg ttcatgaagc gggtgacatg gtggccgcaa
     2881 gcctggctcg gcctgacatt caactggggg gcgctgctcg gtttttctgc ggagaccgga
     2941 tcactcgccc ttcccgcctg gcttctctat gccggctgtt tcttctggac gctcggctac
     3001 gacacgatct acgcgcatca ggacgccgag gacgacgcgc tggtcggcgt tcaatccacg
     3061 gcgcgcctgt tcggcgagcg ctctggtccg tggatcgcgg cattctatgc gtgcgcgatc
     3121 gcctgcatcg gcgctgcgat gtggttttcg atggcgcgga cttggtcatt gttcttgctc
     3181 gctcccgcag gcttgcactt tctgtggcaa gtgcgcaggc tcgaccatgg tgacaccaca
     3241 cgctatctga caattttccg ttccaaccgc gacacaggac tccttatcgg cgcagccctg
     3301 gtggctgcaa gctatgtaca ctaaaaaaga tctcaacgca gaggacgctg aggttgcgca
     3361 gtgggtgcag aagaagttgc tgcgcgccgt aagcgcgatc ctcctgcccc gcgtcctctg
     3421 cgcatccttc gcgccctccg tgttaaatct attctgtgaa aggaaatccc catgtcccag
     3481 ttcgttgcgc cgcaatccat cgtcgcgctg gtgctttatg cgctgtgggc gattgcactg
     3541 gtgctgatgg tcggcgccga ccgcgtgctc atggtgatcc gcggcaaagc gaaagcggac
     3601 agcttcacgc ctggcgtgcc gcatggcggc gattcctact ggcgcatcaa tcgcgcgcat
     3661 gtgaacacgg tcgaaaatct gccgatcttt gcggcgatcg tgctggccgg ctgggtcgtg
     3721 ggcatggaga cggcgacgtt caaccggctc gcaatcatcg tcgtcgttgc gcgcattgtt
     3781 caatccgcta tccatatcgc gtcgggatcg gttgtcgcga ttacttttcg cttcacggcc
     3841 cttgccattc aactgatctg cgaaatctgg atggcgatcc tggtgctccg tgcgggcggt
     3901 cttttctgag cgaaacagac aacgcgccct cttggccatt gtctgccgcg tcgggtaaaa
     3961 tccacggtcc ctggaataaa gcggtgcgcc ggtccgtcga gaatatcggg caccatcgtt
     4021 atgaccgaaa catgatgcaa gggcatcccg cggagttcat ccgcgccaat accgagcttg
     4081 ccgcgccgcc tctcattccc gagatcgacc tgcatcttgc gaccgaagtt gtgccgttat
     4141 ggcgcaagac ggaagaggaa ttgcagcggg aaggcgtgcc gccgccctat tgggcatttg
     4201 cctgggctgg cggtcaggcc ttggcccggt acgtgctgga ccatcgcgaa acggtagcaa
     4261 cccgggccgt actcgacctt ggcactggct cgggccttgt cgccatttcc gccgcaaagg
     4321 cgggcgcgcg gagcgtgctc gccgccgatg tcgacgaatt tgccgccgca gcgatcgccc
     4381 tgaattgctg ggcaaacggc gtgagctgcg ttacgcgccg gcccgccgcg aaacgtccgg
     4441 cagtcgccag gcggggcgaa aaaatagaaa tcaccaccga cgacgtgatc ggacatcgat
     4501 gcgcgtggga cgtcattctg gtgggggaca tgtgttacga gcggccgctc gccgagcgcc
     4561 tgctccaatg gctcagccag tgcgccgaag gcggcacgca ggtttttctg ggtgatccgg
     4621 gacgcgccta ttttccggaa agcggaacgg tccggcttgc gacctatcgc gtgccaacct
     4681 cccgcgaact cgaagaccgc gagatccgcg agacgagtgt cttccggctg gggtcaatga
     4741 atggcgcact gggtctcggc ggtgcttgac gccggcgcga aaggatggtt ttctgaggaa
     4801 caaacaggga acctgtcaat gccggtggac ggctttctcg actacatcga acttcccggg
     4861 cccgacattc cggcaacgaa gcggttctat ggctcgctgt tcggctggac cttcaccgat
     4921 tacgggccgg actatgttgc gttcgagtcc gccggacgcc agggcggctt caacgcggag
     4981 cggaa
//
LOCUS       DASZMC010000438         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1581809,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000438 DASZMC010000000
VERSION     DASZMC010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581809"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..721
                     /locus_tag="VG274_09090"
     CDS             326..721
                     /locus_tag="VG274_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06850.1"
                     /translation="MMTNFRKMRLIEHCHSFASWLLKRPRAWSFAAYALQASAIVFSG
                     ISAFLLRFEFTLPAVARPCFVWGLLVWITINPLILRWSRVVGGWRHFSTSDFGRLLPA
                     NAACLAASALVLAFACPCPSGARSCSSKP"
     gene            718..>1948
                     /locus_tag="VG274_09095"
     CDS             718..>1948
                     /locus_tag="VG274_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HWE06851.1"
                     /translation="MIYLLLSSGVRVATRISFQLAAQTLRGDDLAHIFKRRGIARILI
                     AIPSATVSQMREIVARCHEAQISFRTMPPISEILLDRGLTRQIRDVAVEDLLGRGSVR
                     LDDETIRAKIEDRVVLITGAAGSIGSELCRQVARYRPAMLAGFGVAERALFYLERGIS
                     EAFPDLDFRAEIGIIQNPERLRELFAAWRPSLVLHAAACKHVPLMEKHMFEAAGNNIL
                     GSSNPGRIAGEFAIEHFVMISSDNAVNPTDIMGAGKRAAELLIRSLQNRRARYISVRF
                     GNVLASNKSVVPIFKQQVAAGGPTTTTHPDMQRYFMTIPETAWLVLQACAMGRGEEIF
                     VLDTGKPVKIVELARQLIRLFGLEPDEDIRIEFTRMRPGEKLYEEFPTDGENILPTDH
                     EKIKRFAGSGLSPERAAA"
BASE COUNT          338 a          710 c          504 g          396 t
ORIGIN      
        1 gtttcaaccg gcgcccatgc ggaatatccg gatacggctc gatggagcgt acaaaagcgc
       61 cgaatccatc cgcagcccag gccgcctgcc gctcgaggcg catggcggtt attcggagtt
      121 cgtcctccct tctctttccg attacgagat catcgtgctc cgaaactaag tctctggaaa
      181 attccccttc gcgaattctt tattccccgg agaatttgcg ccgcacaatt cactttctcg
      241 atgtaccggc aattccgggt ctgaacgcgc ggctctcggg agcgaattcc cgtggctcgc
      301 gttccgcggc cgccactcga gtgaattgat gaccaacttc cgtaagatgc ggttgattga
      361 acactgccat tccttcgcgt cctggctttt gaaacggccc cgggcctggt ccttcgccgc
      421 ctacgcgctg caggcgtccg ccatcgtttt ttcgggcatt tcagccttcc tgctgcgctt
      481 cgagttcacc ctgcctgccg ttgccagacc ctgcttcgtt tggggtctgc tcgtttggat
      541 caccataaac ccgctgatcc tccgatggtc cagagtagtc ggcggctggc ggcacttctc
      601 cacgtccgat ttcggccgtc ttcttccggc caacgccgcc tgtctggccg cctccgcgct
      661 cgtgctcgct tttgcctgcc cctgcccttc cggcgctcga tcctgctcat cgaagccatg
      721 atttatctgt tgttgagcag cggcgtgcgc gtcgccactc gcatctcctt ccaactcgcc
      781 gcccagacgc tccgcggcga cgatctcgcc catatcttca agcgccgcgg aatcgcccgg
      841 atccttatcg ccatcccgtc cgccaccgta agccagatgc gcgaaattgt cgcgcgctgc
      901 catgaggccc aaatcagctt ccgcaccatg ccgccgattt ccgaaattct gctcgatcgc
      961 ggcctcaccc gacagattcg ggacgtggcc gtcgaagacc tcctgggccg cggcagcgtc
     1021 cgtctcgacg acgaaacaat ccgcgccaaa atcgaggatc gcgtcgtcct cattaccggc
     1081 gccgccggct ccatcggctc cgaactttgt cgtcaggtgg cgcgctaccg gcccgctatg
     1141 ctggccggct tcggcgtcgc cgaaagggcc ctcttttatc tcgagcgcgg aatctctgaa
     1201 gccttccccg atctcgactt ccgcgccgaa atcggtatca tccaaaaccc ggagcgcctg
     1261 cgcgagctct tcgccgcctg gcgccccagc ctcgtccttc acgccgccgc ctgcaaacac
     1321 gtgcccctga tggaaaagca catgttcgag gccgccggta acaacatcct cggctcctcg
     1381 aatccgggcc ggatcgcggg cgaattcgcg atcgagcatt tcgtcatgat ctcctccgac
     1441 aacgccgtca atcccaccga catcatggga gccggcaagc gcgccgccga gcttctcatt
     1501 cgctcgctgc agaatcgccg cgcccgctac atctccgtcc gcttcggcaa cgtgctcgcc
     1561 agcaacaaaa gcgtcgttcc catcttcaaa cagcaggtcg ccgccggcgg ccccaccact
     1621 accacgcacc cggacatgca gcgctacttc atgacgatcc ccgagaccgc ctggcttgtc
     1681 ctgcaagcgt gcgccatggg ccgcggcgaa gaaatattcg tcctcgacac ggggaagccc
     1741 gtgaaaatcg tcgagctcgc gcggcaactg atccgccttt tcggcctcga gccggatgaa
     1801 gacatccgca tcgagttcac ccgcatgcgc cccggagaaa agctctacga agagttcccc
     1861 acggacggcg aaaacatcct ccccaccgat cacgagaaga tcaaaagatt cgccggctcc
     1921 ggcctttccc cggaacgcgc cgccgccc
//
LOCUS       DASZMC010000439         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2655706,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000439 DASZMC010000000
VERSION     DASZMC010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655706"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VG274_09100"
     CDS             <1..572
                     /locus_tag="VG274_09100"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE06852.1"
                     /translation="MDVTDAASIAAAFANIATLDILVNNAGIGLVGDITRTSEEDFAR
                     VMRVNVHSVFLVTQAAFPLLLASRGSIVNIGSVAATVGVKQRFAYCASKGAVLAMTRQ
                     IAVDYPKELRINCIAPGTVQTPFVEGYLDKYHAHEKEKVRAELVARQPIGRLGTPEDI
                     ASLVRYLCSREAEFINGALIPIDGGWTAA"
     gene            652..1956
                     /locus_tag="VG274_09105"
     CDS             652..1956
                     /locus_tag="VG274_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWE06853.1"
                     /translation="MNDLLITAVRVIDLRFPTSRENIGSDAVNKDPDYSAAYCIIETN
                     SALEGYGLSFTLGRGTDLVVQAAEYLSRYVINRTLASITDDFRAFARELTDDTQFRWL
                     GPEKGVIQLAAAALINAVWDLYARAESKPLWQLLAEMEPERLIGAIDFRYIDDALSPE
                     EALEILTSRRKGQAERLALLQQKGYPAYTTSVGWFGYSEEKIRRLSKEALADGWTYFK
                     LKVGGDAADDLRRGHIVREEIGWSNKLMVDANQKWGVVEAIARTRQLAELKPWWMEEP
                     TNPDDILGHARIRREVPEVRIATGEHVHNRIMFKQLLQAEAIDVLQLDSCRVAGVNEN
                     LAIMLMAAKFGVPVCPHAGGVGLCEYVQHLSVFDFLSVSATLEDRVIEFVDHLHEHFV
                     DPVRIKDGHYLLPRQPGYSIQIREESLTRFAYPQGEAWSASK"
BASE COUNT          365 a          568 c          637 g          391 t
ORIGIN      
        1 caatggacgt caccgacgcc gcgtcgattg cggctgcgtt cgcgaacatt gccacgctcg
       61 acatcctggt caacaatgca ggcatcggcc tggtgggcga tatcacgcgc acgtcggagg
      121 aggactttgc ccgcgtgatg cgtgtgaatg tgcacagcgt ttttctggtg acgcaggcgg
      181 cgttccctct gctgctggcc tcgcgcggca gcatcgtcaa catcggttcg gtggcggcga
      241 cggtgggcgt gaagcagcga tttgcctact gcgccagcaa gggcgccgta ctggcgatga
      301 cgcggcagat cgcggtcgat tatcccaagg agctgcgcat caattgcatc gctcccggca
      361 cggtgcagac gccgttcgtc gagggatatc tcgacaagta tcacgcgcac gagaaggaga
      421 aggtgcgcgc cgagctggtc gctcgccagc cgatcggccg cctgggcacg cccgaagaca
      481 tcgcttcgct ggtgcgctat ctgtgctcgc gcgaggccga gttcatcaac ggcgcgctca
      541 tccccatcga cggcggatgg acggcagcct gattttttta cgcgacgacc ttaaattcga
      601 cgccgcttaa attcgacgac ccttaaattc aaagacagga aagctgccac gttgaacgat
      661 cttcttatca ccgctgttcg cgtcatcgat ctccgtttcc cgacctcgcg ggagaatatc
      721 ggctccgatg ccgtcaataa agacccggat tattcggctg cctattgcat cattgagacc
      781 aactcggcgc tcgaaggcta tggcctgtcg ttcacgctgg ggcgcggcac cgacctggtg
      841 gtgcaggcgg cggagtatct gtcgcgctac gtgatcaacc gcacgctggc atcgatcacc
      901 gacgactttc gcgcgtttgc gcgtgagctc accgacgaca cacagtttcg ctggctgggg
      961 ccggagaagg gcgtgattca actggctgcc gctgcgctga tcaacgcggt gtgggacctt
     1021 tatgcgcgcg ccgagtcgaa gccgctgtgg caactgctcg cggagatgga gccggagagg
     1081 ctgatcgggg cgatcgactt tcgttatatc gacgatgcgc tgtcgccgga ggaggcgctg
     1141 gagattctca ccagccgccg caagggacag gccgagcggc tggcgctgct tcagcagaag
     1201 ggctatccgg cttacacgac gtcggtgggg tggttcggct atagcgagga aaagattcgg
     1261 cggctttcga aagaggcgct ggccgacggc tggacgtact tcaagctgaa ggtgggcggc
     1321 gatgcggcgg acgatctgcg gcgcgggcat atcgttcgcg aagagattgg ctggagcaat
     1381 aagctgatgg tcgatgcgaa ccagaagtgg ggcgtcgttg aggcgattgc gcgcacgcgc
     1441 cagctcgctg agctgaagcc gtggtggatg gaggagccga ccaatcccga cgatattctg
     1501 gggcatgctc gcattcgccg cgaggtgccg gaggtccgca tcgcgaccgg cgaacatgtt
     1561 cacaatcgca tcatgttcaa gcagctactt caggccgagg cgattgacgt gctgcagctc
     1621 gacagttgcc gagtggcggg ggtgaatgag aaccttgcca ttatgttgat ggcggccaaa
     1681 ttcggtgtgc cggtctgccc tcatgccggg ggcgtggggc tgtgcgagta tgtgcagcat
     1741 ctctccgtct tcgacttcct gagcgtctcg gccacgcttg aagatcgcgt catcgagttt
     1801 gtcgatcatc tgcacgaaca cttcgtcgat cccgtccgca tcaaggatgg gcactacctt
     1861 ctgccgcggc agcccggcta cagcattcag attcgcgaag aatccctaac ccgcttcgcc
     1921 tatccgcagg gcgaagcctg gagcgcctcc aagtgaaatt c
//
LOCUS       DASZMC010000440         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5128976,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000440 DASZMC010000000
VERSION     DASZMC010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5128976"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1632
                     /locus_tag="VG274_09110"
     CDS             202..1632
                     /locus_tag="VG274_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWE06854.1"
                     /translation="MKFSEYVKYDGLGLAELVRQKDVTAAELADIAIARAEAVNPALN
                     FLVYSDFDRARVAARRDPPSGLFAGVPFLLKDILAFAQGMPTRQGARFIPVIPFPHDS
                     VLTTRFREAGLIFLGKTNVPEFGLLPLTESKLYGPARNPFALDRSTGGSSGGSAAGVA
                     AGVVPLAHANDGGGSIRIPASCCGLVGLKPTRGRGTLAPDMGEAVDGLAVDHVLTRSV
                     RDTAAALDVAGANIQGDPYWAPPGPSSWLAAHEAKPRRLRIAFSLRKLDGGGLHADSV
                     AAVQSAAKFCAELGHIVEEATPQFDLTALVPAFMAVWTANLAAAIDFTAMVTYQTPAA
                     ELFEGLTWGMYEAGKRVSGSEYLIAKEALHRAARGAAKFHESHDLWLAATLGTPPMKI
                     GTFNIDERDIGKGFVPLFDYVPFTALQNVTGQPAINLPLFWNAEGLPIGVQFAAPFGD
                     ELTLLQLAAELERAAPWSHRYANIKV"
     gene            1599..3068
                     /locus_tag="VG274_09115"
     CDS             1599..3068
                     /locus_tag="VG274_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWE06855.1"
                     /translation="MVASLREYQGLNVGFAEYGRHDATGLAQLVRRREVTPRELIEEA
                     IARIERHNPKLNAVVYKAYDEARAAADGKLPDGPFRGVPFLIKDMDLPVAGWPLANGS
                     AFLRNNRSTGDAELARRYRAAGLVLIGKTNVPEFGIPGTTEGRYYGVCRNPWNPDHSA
                     GGSSGGSAAAVASGMVPIAHGSDGLGSIRIPAAQCGLVGLKPTQYRNPGGPDDRGRAN
                     GLVVDHVLTRSVRDSAAMLDWTGYPEDDAPYAPAPKMRPYTDELRTLPGRLRIAFTGA
                     TTHGGALHKDVQAVLDATAALLDELGHTMIEKASLDIDWRALYRAQGAVSGAMFAANI
                     DSWAETVGHAPREDELEPLAWAAWRAGKERTATEVGEGLRTLRLLTRRILALWRDFDV
                     LLTPVTITPAPPIGHLDPVNVEPREFNRRQGRAFNFTPPYNMTGQPSLSLPLAMSPDG
                     LPIGMMFTARTADEATLFRLAAQLEQARPWGNRHPPIWD"
BASE COUNT          531 a         1051 c         1004 g          545 t
ORIGIN      
        1 gcgcaaacca cctcccccac gatgggggag gaaaaccaag tgcttcacgt ctgtgaaaat
       61 gcatgatgat tgttattgct tcaatgtccg cacattggcg ccctggcgat tggttctctc
      121 aatccgggat cgtgtcccaa agctccgtga caggctgtca ggcgatggct aaactttggt
      181 gacctcgata cgggcgcaaa catgaagttt tccgaatatg tgaagtatga cgggcttggg
      241 ctggccgaat tggtgcgcca gaaggacgta actgcggccg agctcgccga catcgccatc
      301 gcacgcgccg aggctgtgaa ccccgcgctc aattttctcg tctacagcga tttcgaccgg
      361 gcccgcgtgg cggcacggcg cgacccgccg agcggattgt ttgccggcgt tccatttctc
      421 ctgaaggaca tcctcgcctt cgcgcagggc atgccgacgc gtcagggcgc gaggttcatc
      481 ccggtcattc cgttcccgca cgattcggtt ctcaccacgc gtttccgcga ggccggactg
      541 atttttctcg gcaagacgaa cgtgcccgaa ttcgggctgc tgccgctgac ggaatcgaag
      601 ctgtacggac cggcgcgcaa tccctttgct ctcgaccgct cgaccggcgg ctcttccggc
      661 ggctcggcgg cgggagtcgc agcgggcgtc gtgccgctcg cgcatgccaa tgacggcggc
      721 ggttcgatcc gcatcccggc ctcgtgttgc gggctcgtcg gactgaagcc gacgcgcgga
      781 cgcggcacgc tggcgcccga catgggcgag gccgttgacg gcctcgccgt cgatcacgtg
      841 ctgacgcgca gcgtgcgcga taccgcggct gcgctcgacg tcgccggcgc aaacatccag
      901 ggcgatccct attgggcgcc acccggacct tcgtcctggc tcgccgccca tgaggcgaaa
      961 ccgcgccggc tgcggatcgc tttttcgctc cgcaagctcg acggcggcgg gttgcatgcc
     1021 gacagcgtcg ccgccgttca atctgcggca aagttctgcg cggagctcgg acacatcgtc
     1081 gaggaagcaa cgccgcaatt cgatctcacc gctctggtgc ccgcctttat ggcggtatgg
     1141 acggcgaatc tggcggccgc aatcgatttc accgcaatgg tcacctatca gacgcccgcc
     1201 gcagaactct tcgaggggct gacctggggc atgtacgaag ccggcaagcg cgtcagcggc
     1261 agcgaatatc tcatcgccaa ggaagcgctg caccgggcgg cacgcggcgc cgcgaaattt
     1321 cacgaatccc atgatctctg gctggccgcg acgctcggca cgccgccgat gaagatcggt
     1381 acctttaata tcgacgagcg cgacatcggc aaaggattcg tgccgctgtt cgactatgtg
     1441 cccttcaccg cgcttcagaa cgtcaccggg cagccggcaa tcaatcttcc gctgttctgg
     1501 aacgcggaag gcttgccgat cggcgtgcag tttgccgctc cgttcggaga cgagctgacg
     1561 ctgcttcagc tcgcggccga actggaacgc gcggcgccgt ggtcgcatcg ctacgcgaat
     1621 atcaaggtct gaacgtgggg ttcgcggaat atggccgtca cgacgcgacc ggcctcgcgc
     1681 agctcgtgcg gcggcgcgaa gtaacgccgc gcgaacttat cgaggaagcg atcgcgcgca
     1741 tcgagcgcca caatccgaaa ctcaacgcgg tcgtctacaa ggcctatgac gaggcgcgcg
     1801 ccgctgcgga cggaaaactt ccagacggac cgttcagggg cgtgcccttt ctcatcaagg
     1861 atatggacct tcccgttgcc ggctggccgc tcgcgaacgg ctcggccttt ttgcgcaaca
     1921 accgctcgac gggcgatgcg gaactggcgc gccgctatcg cgccgcgggt cttgtgctca
     1981 tcggcaagac caatgtgccg gagttcggga ttccgggcac gacggagggg cgttactacg
     2041 gcgtctgccg caacccgtgg aatccggatc acagcgccgg cggatcttcg ggcggctccg
     2101 ctgctgcggt cgcgtcgggc atggtgccga tcgcgcatgg cagcgacggt ctcggcagta
     2161 tccgcattcc cgccgcgcaa tgcggccttg tcggtctcaa gcccacccaa taccgcaatc
     2221 ccggcggccc cgacgaccgc ggccgcgcca acggactcgt ggtcgatcat gtgctgacgc
     2281 gcagcgtgcg cgacagcgcg gcgatgctcg actggacggg ttatcccgaa gacgacgcgc
     2341 cctacgcgcc ggcgcccaag atgcgtccgt acacggacga actccgcacc ctgcccggcc
     2401 ggctcaggat cgcgttcacc ggcgcgacca cgcatggcgg agcgctgcac aaggatgtgc
     2461 aggccgttct cgatgcgacg gccgcgctgc tcgacgaact tggccacacc atgatcgaaa
     2521 aggcctcgct cgatatcgac tggcgcgctc tctaccgcgc gcagggggcg gtctcgggcg
     2581 cgatgtttgc cgcgaatatc gattcctggg cggagacggt cggtcacgcg ccgcgcgaag
     2641 acgagctgga gccgctcgcc tgggcggcct ggcgcgcggg caaggagcgg accgccacgg
     2701 aagtgggcga agggttgcgg accttgcgcc tgctcacgcg ccggattctc gcgctgtggc
     2761 gggatttcga tgttctgttg acgcccgtca ccatcacgcc ggcgccgccg attggccatc
     2821 tcgacccggt gaacgtggag ccgcgcgaat tcaacaggcg ccagggccgc gcattcaatt
     2881 tcaccccgcc gtacaatatg accggtcagc cttcactctc gctgccgctc gcaatgtcgc
     2941 ccgacggact accaatcggc atgatgttca ccgcgcgcac cgccgacgag gccactctct
     3001 tccgcctcgc cgcgcaactc gaacaagcgc gcccctgggg caatcgccat ccgccgatct
     3061 gggattgagc agcggcggtc gacgggccgt attccggcgg acggtcgcac cagcgacaac
     3121 cgcttttgag c
//
LOCUS       DASZMC010000441         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5129350,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000441 DASZMC010000000
VERSION     DASZMC010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5129350"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..546
                     /locus_tag="VG274_09120"
     CDS             223..546
                     /locus_tag="VG274_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06856.1"
                     /translation="MFARAGAVGADPDRASPAIATGTEAVPSAEPEPVDPASDLARCF
                     LRLANLPSVALDRLSRYEAILWRQVGQILFALDALDRRKLQDRGRRSRIGSRQTLPAD
                     GGDDY"
     gene            846..1133
                     /locus_tag="VG274_09125"
     CDS             846..1133
                     /locus_tag="VG274_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWE06857.1"
                     /translation="MDITKDIQAMTTFRNNSAKFLRHLRKTKRALVLTVNGKAAAVVQ
                     DAEAYQHLLDLAAESSAAEGIRQGFEDVANGRTRPAREILDEIRAEYDIPR"
     gene            1117..1479
                     /locus_tag="VG274_09130"
     CDS             1117..1479
                     /locus_tag="VG274_09130"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWE06858.1"
                     /translation="MTYRVELTARAGRNLKRIFRHINAESSPQASVWFNELEAAILSL
                     DEHPERSPATPENPALRNLLFGKKPNVYRVIFSIDARARVVWVLHIRHGARRPLPRRP
                     SDRAGDVLEGRKVRAVGR"
BASE COUNT          431 a          658 c          672 g          392 t
ORIGIN      
        1 cttagctgcc catagaccta cggttgccgc gctccgaggt gcgctcgaag cagccggaat
       61 cgaatttacg aacggggatc aagcgggcgt gcgactccga cgcaagactt gaggtcattc
      121 gccctaattt ttcgactcgt ccgatcacgg tgctggccca tgttccgggg aatggggcag
      181 acccgccaaa ttcacccgga ctcacgaaac atagtctgcg cgatgttcgc ccgggctggc
      241 gcggtcggtg ccgaccccga tcgagcctcg cccgccattg caactggaac ggaagctgtg
      301 cccagcgccg agccggaacc cgtcgatccc gcctccgacc tcgcccgctg cttcttgcgc
      361 ctggccaatc tgcccagcgt cgcactcgat cggctcagcc ggtatgaagc gatcctgtgg
      421 cgccaagtcg gccaaatcct gtttgccctc gacgcattgg atcgtcgcaa actacaggat
      481 agagggcgcc gttcacgtat cggtagccgg caaacactgc cggctgatgg aggcgacgac
      541 tattgagctg cgcgggcccc accggtctac gccatcggcc actcggccgg aacaggcgag
      601 cgatcggaat catcatcaac gaaattgggt ttgtttcgtc aaatcccatg gtcagcccaa
      661 tctctgctat ggcaccgctc aggcaacccg acttgatctg ggcacattgc cgcaacgtcc
      721 gagttgaggt ttccgacact gacatggcag atgaagaatg tttagggcca cccacgaatc
      781 cttgtgcaat ggcgcttggc ctatggtatt accgaattcg gtagcgccag ggagaaggtc
      841 gcgcgatgga catcaccaaa gacattcagg ccatgaccac cttccgaaac aactcggcta
      901 aatttctgcg ccacctgcga aagaccaagc gggcgttagt tctcactgtg aatggaaaag
      961 ccgccgccgt ggtgcaggac gccgaagcct atcagcacct gctcgatctg gcggcggaat
     1021 ccagcgcggc cgagggcatc cggcagggat ttgaagacgt tgccaatggc cggacacggc
     1081 cggcgcgcga aattctggac gagatacgcg ccgaatatga cataccgcgt tgaactgacg
     1141 gcgcgggcgg gccggaatct caaacggatc tttcggcaca tcaatgcaga aagctcgccg
     1201 caggcgtccg tgtggttcaa cgaactcgag gcggcgatcc tgagcctcga cgagcatccg
     1261 gaacgaagcc cggcgacgcc agagaatcct gcgctgcgga acttgctttt cggcaaaaag
     1321 ccgaacgttt atcgggttat cttttcgatc gacgcgcgcg cccgggtcgt gtgggtgctg
     1381 catatccgtc acggggcgcg ccggccattg ccgagacggc cgtcggatag agccggcgat
     1441 gtactggagg ggcgtaaagt ccgggcggtt ggacgttgag gccgtgcggc cccaccgccg
     1501 gcgtggagtt catcgaccgg aaagggggtg gaccgggggt tccgcaccga aatcctcgct
     1561 gctatcgata tcgcgaaagc ctcggttgat cgcggcgaag gcaccgaaat cacccgggaa
     1621 tccatgcgcg cgctagctga ggacgtaaaa cggcgcggcc ggacgcagct tgccgccgag
     1681 catcacccca cgggggtaat gtggtgcatc gcctggcacc gcaggcgcgg actgaactct
     1741 cagcagcatc tggaattata tcgtcaaaga aggcggcaat gtcgccgccg ctgatggcgt
     1801 cttcgacgca atcacggagc gcttctacct gctcagccgg taccctcgca tcggccgctc
     1861 tcgcgacgat ttacgccccg gatttgcgca gcttttgcgc atcgacgcag aactcggccg
     1921 ggaccgacga gcaatgggaa tatcgtggct gacgaaaatt gggtttgttt tgcctctgct
     1981 tcgcgacatg cggaggcggg cgtttccgcc cgagctgcgc gtgcaatggg ctcgacagcc
     2041 agcggtgatg gcggcatgaa gccgtgggct gccgtcgtat atccgaacgc caccagcctg
     2101 ctgatcaccg ccgatggcgg cggcagcaat ggttcgcgcg tgcgattgtg gaa
//
LOCUS       DASZMC010000442         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4319853,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000442 DASZMC010000000
VERSION     DASZMC010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4319853"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VG274_09135"
     CDS             <1..323
                     /locus_tag="VG274_09135"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWE06859.1"
                     /translation="ARFLSPFDPAIRDRKRARRLFGFDYTIEVYVPEKKRRYGYYVLP
                     ILEGEHFIGRADLKAHRGEHRIEVKGTWSEPGVDFDKRRRDAFDAALAELSRFTSAGS
                     ELQA"
     gene            373..2529
                     /locus_tag="VG274_09140"
     CDS             373..2529
                     /locus_tag="VG274_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWE06860.1"
                     /translation="MKQVARMIGALALAAASAGAASLRPPAAPKIPVIDDYFGYRITD
                     NYRWMEDRSSPKFVGWIRKENAYARAVLARIPGRDALLRRIAAHTGGGTAISGVQLAG
                     GRVFYLRREPNEDTFKLFMRPSISGAEKLLVDPTRRDANGKHFAIDYFQPSQNGAMLV
                     YGISEGGSENSVIHILDTRSGIESPETINRCEDAGPSWRPDGKSFFYRREAKLEPGAN
                     ETDKYLNSRALLHVVGADPENDVPIVGTGVAGSPALTPASLPFVVVSPGSRYALAAIS
                     PGSIPEAEYYIATTAQVTDGHAPWKRVASLGDKVTGAALHGDRLFLITYKNAPRFKVV
                     ETSAVSPDIANAKTVVPAGTRVIEDIAASSDALYIRDLDGGPSRLRRYDFASGRIGEV
                     VLPADGTLTGPATDSISPDVLFGLQGWVIPPRWYALAKGRVAALALAPPWKDDYSAFV
                     AEEVKARAHDGTLIPLSIVHKRGLKLDGTNPVWLIGYGAYGLSLTPALVSRFVPLLED
                     GGVVAIAHVRGGGEYGEEWHMAAHLATKRKSYTDLLDCALYLEQNHYGSADTMAIEGR
                     SAGGITVGMALDAAPTLFRVVFSGVGDSNALRAEFETDGAANSLEYGSVKTEDGFKAL
                     ADVDAVLHVMGGVAYPAVMLTTGMNDPRVAPWQPGKMAAALQAASSSGNPIILRVDFE
                     AGHGFGSTRSQRDAEMADQMAFFYWQIGKRGYQPRH"
     gene            2689..5793
                     /locus_tag="VG274_09145"
     CDS             2689..5793
                     /locus_tag="VG274_09145"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06861.1"
                     /translation="MGLDATWNLVGIPADARDAARAAALREGLSIGDWLTRRILRSFS
                     ELNFHEHEESFDRLSDNLIELSDRLERIENRADAEPMREAVKALHQGLSRLTDDLVKT
                     AGHSAVQISSLSGKLENLNEELAQFRTLDSGISGAFDERVSRVQKGLESLDARQSDAS
                     RSLDERQTVLARKLEEAKSRAAGLSSSLEHRIAHLQRGLEGLDARHGDDCRSLAGRLE
                     TLSGAVDDVRSDGAGTRSALDQRLALVHQGLEHLNARQEDESRTWTGRLEEVRTESTS
                     RCSALDQRLASVLQRLQGLERLENYLSGSLTGKFDEIRAETSGKFGAFDESLATVRQK
                     LENLDAHGNELSQALTGKLELLTGQVSAVRTESSGAVTALERRVASVHEHLTSVDATQ
                     NDTLQILSRDLETFTKKLDDVRSVASGASTALDIRAAQIQQSLDSLDRRQNDRSRALS
                     EMRDREAANLAVVVNLEVSVAELKSQLSDLAPDSRLVALERSVSELASRTETVEHALS
                     ALQPKANPVDGMLKSLSGSFEVHTDTPPSEIPRDAIHLDASAESKASQFGEAGAASGD
                     AHNVTDQPELNADANDSQTREMPTVSDDGAQPATLFGLKDGVQGEALTAFAAKMQVSS
                     TPARDPTATLEPVSFPSASLPEPEPEQTHSDTTTPLPPFPDTRTAVSVLDPQAGLPPD
                     RAPAIAAFARSDSESSASGPISYLSAARQSLQAAAVHGEADTGSYSLFGLHFFDAERA
                     HAERKSERTSYALLAAIVLVAILAVAVAAGEWLRHSTPAKGLRIGPAVARLHMKAPAA
                     PHPKARGAAHGVRGQSAAIATSITRPAATNSQKPAMPNDLVRIVALANAGEARAQMIL
                     GLHDLDDGKGGNPEEAAKWLERAAEQGEPIAQYRMATMYAAGRGVKADDAKAFHWYEA
                     AAQAGNRKAMSNLAVAYAQGKGVGKNPQEAVRWFSKAAELGLVDAQFDLAILYERGLG
                     VRQSLSNAYRWYVIAARAGDKESRDRIDAIASQLSASDRATGEKSAAEFKSAPMNPEA
                     NESK"
     gene            complement(5906..6157)
                     /locus_tag="VG274_09150"
     CDS             complement(5906..6157)
                     /locus_tag="VG274_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWE06862.1"
                     /translation="MSILAWIILGLIAGFIGSKIVNKSGEGLILDIVLGIVGAIVGGW
                     LFRFFGSTGVSGLNLWSMLVAIIGAVVVLVAYHAIRRVA"
BASE COUNT         1274 a         1970 c         1918 g         1037 t
ORIGIN      
        1 cggcgcgctt cctgtcgccc ttcgatcccg cgatccgcga ccgaaagcgc gcgcggcggc
       61 tcttcggctt cgattacacc atcgaggtat acgtgccgga gaagaagcgg cgctacggct
      121 attacgtgtt gcccatcctc gagggcgagc acttcatcgg ccgcgccgac ctcaaagcgc
      181 atcgcggcga acaccggatc gaggtgaaag gaacctggtc cgagcccggc gtcgatttcg
      241 acaagagacg ccgggatgca ttcgacgccg cgcttgcaga gttgtcgcga ttcacgtcgg
      301 ccggctccga actccaggct tgaccacgcc gacgcggttt cattttatcg cacccatccc
      361 gggagatttt gcatgaaaca ggtcgcacga atgattggag cgcttgcgct cgccgccgcg
      421 tctgcgggcg ccgcatcttt gcggccgccg gctgcgccga agatacccgt catcgacgat
      481 tatttcggct accggattac cgacaattac cgctggatgg aagaccgctc ttcgccgaaa
      541 ttcgtaggct ggatcaggaa ggaaaacgcc tatgcgcggg cggtgctggc gcgcattccg
      601 ggccgcgatg cattgctcag gcgaatcgcg gcccacacag gcgggggaac ggcaatctcg
      661 ggcgtgcagc ttgcaggcgg ccgcgtcttc tatctcagac gcgagccgaa cgaggatacg
      721 ttcaagctct ttatgcgccc ttcgatatcc ggcgcagaaa agcttctcgt cgatcccacc
      781 cgcagagacg cgaatggaaa acacttcgcc atcgattatt tccagccttc gcagaacggc
      841 gccatgctcg tctacggtat ttccgagggc ggctccgaga acagcgtcat ccacatcctc
      901 gacacgcggt ccgggatcga atcgcccgag accatcaatc gttgcgaaga cgcggggccg
      961 tcgtggcgcc cggacggaaa gtcgttcttt taccgcaggg aggcgaagct tgaacccggc
     1021 gcaaatgaaa cggacaaata tctgaacagc cgcgccctgc tgcatgtggt gggcgccgat
     1081 cccgagaatg acgtgccgat cgtcgggacg ggcgtcgcgg gatcacccgc gctcacgcct
     1141 gcgagcttgc ccttcgtcgt cgtgagcccg ggctcgcgct acgccttggc ggcgatcagc
     1201 ccaggatcga tccccgaagc cgaatactat atcgcgacca ccgcgcaggt gacggacggg
     1261 cacgcgccgt ggaaacgcgt ggcatcgctt ggcgacaagg tgacgggcgc ggcattgcac
     1321 ggcgacaggc tgttcctgat cacctacaag aatgcgccgc gcttcaaggt cgtcgagaca
     1381 agcgccgtct ctcccgacat cgcgaacgct aaaaccgtgg tgccggccgg cacgcgcgtg
     1441 atcgaggata tcgcggcctc gtcggatgcg ctctatattc gcgatctcga cggcggacca
     1501 agccggttgc ggcgctacga tttcgcgagc ggacggatag gcgaagttgt gctgcccgca
     1561 gatggtacac tgacgggccc cgcgactgac tccatatcac ccgatgttct gttcggcctt
     1621 caaggctggg tgatcccgcc gcgctggtac gcgcttgcaa aaggacgcgt cgcggcgctg
     1681 gcgctcgcgc cgccctggaa agacgattac tcggccttcg tagcggaaga ggtgaaggcg
     1741 cgcgcacatg acggaacgct cattcccctc tccatcgttc acaagcgcgg gctgaaactc
     1801 gacggcacga atcccgtctg gcttatcggc tacggcgcct atgggctttc gctcaccccc
     1861 gcacttgtct cccgcttcgt tccgttgctc gaggacgggg gcgtggtcgc catcgcccat
     1921 gtgcgcggcg gaggcgagta tggcgaggaa tggcacatgg ctgcgcatct ggcgaccaag
     1981 cgaaaaagct acaccgacct gctcgactgc gcgctgtacc tggaacaaaa ccattacggc
     2041 agcgccgaca ccatggcgat tgaaggccgc agcgcgggag gcatcaccgt ggggatggcg
     2101 ctcgacgccg cgcccacact gttccgcgtc gtcttttcag gcgtcggcga ttccaacgcg
     2161 ctgcgcgccg agttcgaaac cgacggcgcc gccaattcgc tcgaatatgg cagcgtgaag
     2221 accgaggacg gattcaaggc gctggcggat gtcgacgccg tgcttcatgt gatgggcggc
     2281 gtggcctacc cggcagtgat gctaacgacg ggaatgaacg acccccgcgt cgcgccctgg
     2341 caaccgggca agatggcggc ggccttgcag gcggcttcgt cgagcgggaa cccgattatc
     2401 cttcgcgtcg atttcgaggc cggtcatggc ttcggctcga ccagatcgca acgcgacgcc
     2461 gaaatggcgg accagatggc gtttttctac tggcagatcg gaaagcgggg ataccagccc
     2521 cgccattgaa gtgcggagtg cgcagtcgcg agcgggaatc gttccgcggc aaacctttga
     2581 tgccccacaa cgcggttaac gcgattttta cgctgttggc acaacattgc cgggaaagat
     2641 gccgcatcgc gcgcgccccg cggatcggca aattcgcgcg aggcaaaaat ggggcttgat
     2701 gcaacctgga atcttgtcgg catccccgca gacgcgcggg acgccgcgcg cgcggccgcc
     2761 ctcagggaag gcctctccat cggagattgg ctcacacggc ggattctgag aagtttctcc
     2821 gaactcaatt ttcacgagca tgaagagagt ttcgatcgtc tcagcgacaa tttgatcgaa
     2881 ctctccgacc gtctggagcg gatcgaaaac cgcgcggacg cagaaccgat gcgcgaagct
     2941 gtgaaagcgc tccatcaagg cctgtcgcgc ctgaccgacg atcttgtgaa gacggccggc
     3001 cactccgcgg tccagatctc gagcctgtcg gggaagctcg agaacctgaa cgaagaactc
     3061 gcgcagttcc gcaccctcga ttcgggaata agcggagcgt tcgacgagcg cgtctcgcgt
     3121 gtacaaaagg gcctcgaaag tctcgatgcc cgccaaagcg acgcatcccg gtctctggat
     3181 gaaaggcaga ccgtccttgc aagaaaactc gaggaagcca aatcccgagc cgccggactt
     3241 tcctcttcgc tcgaacaccg catcgcacat cttcaacggg gcctggaggg cctggatgcg
     3301 cgtcacggcg atgattgccg ctctttggcc ggcagacttg agaccctgag cggagcggtc
     3361 gacgacgtcc gcagcgacgg cgcaggaacg cgcagcgcac tggatcaacg actcgctctc
     3421 gtacaccagg ggttggaaca cctcaatgcg cgtcaggaag acgaatccag gacatggacc
     3481 ggcaggctcg aagaagtccg gacggaatcg acttccaggt gcagtgcgct cgatcagcgt
     3541 ctcgcttccg tgctgcagag gctgcaaggt ctcgaacgtc tcgaaaacta tctgtcggga
     3601 tcgttgacgg gcaaattcga cgagatccgc gcagaaacat caggcaagtt cggcgcgttc
     3661 gacgagagtc tcgcaaccgt tcggcagaaa ctggaaaatc tcgacgcaca cggaaacgaa
     3721 ctgtcgcaag cactgaccgg aaaactcgaa ctcctgacgg gacaggtttc cgctgttcgg
     3781 actgaatcgt cgggtgcggt gacagcgctc gagcggcgag tcgcatcggt gcacgagcat
     3841 ttgacgagcg tggacgcgac acagaacgat acgctccaga tactgtcgcg cgatctggaa
     3901 acgttcacca aaaaactcga cgatgtccgc agcgtggcct cgggagcatc gaccgctctc
     3961 gacattcggg ccgcacaaat ccagcaatcc ctcgactctc tcgatcgccg tcagaacgac
     4021 cgatcgcggg cgctgtccga aatgcgcgac cgcgaggccg ccaatctggc agtcgtcgtg
     4081 aatcttgagg taagcgttgc cgaattgaaa tcgcagcttt cggatctggc gccggacagt
     4141 cggcttgtcg ctctcgaacg aagcgtctcg gaactcgcaa gccggaccga aaccgtcgaa
     4201 cacgcgctgt ccgcgctgca gccgaaggcg aaccccgtcg acggaatgct gaaatcgctg
     4261 agcggaagct tcgaggttca caccgacacg ccaccaagcg aaattccgcg ggacgcaatt
     4321 catttggacg ccagcgccga atcgaaagcg agccagttcg gcgaagcagg agcggcatcc
     4381 ggtgacgcgc acaacgtcac cgaccagccg gaattgaacg cggacgcgaa cgacagtcag
     4441 acccgcgaga tgccgacagt gtccgacgac ggcgctcagc ccgccacatt attcggattg
     4501 aaagatggag tgcagggaga ggctctgacg gcgttcgctg ccaaaatgca ggtgagctcc
     4561 acacctgcgc gggaccctac cgcgacgctg gaaccggtct cattcccgtc cgcgtcactg
     4621 ccggagcccg agcccgagca gacccattcc gacacgacga cgccacttcc gccgtttccg
     4681 gacacgcgca ccgcagtgtc ggtgcttgac cctcaggcgg gcctgccccc ggaccgggcg
     4741 ccagccattg ccgcgttcgc gcgcagcgac tccgaatctt ccgcctcggg cccgatctca
     4801 tatctctccg cggcgcggca gtcgctgcag gcggccgcgg tccatggtga agccgacact
     4861 ggttcgtaca gtctgttcgg cctgcatttt ttcgacgcgg agcgagcaca tgcagagcgg
     4921 aaatccgaaa ggacgagcta tgcactcctc gccgcgatcg tgctcgtcgc catcctggct
     4981 gtcgccgttg cggcgggcga gtggctcagg cactcgactc ccgcaaaggg attgcgaatt
     5041 ggtccggccg tcgcgaggct gcacatgaaa gcgccggctg ctccgcatcc gaaggcacgc
     5101 ggcgccgcac acggtgtccg cggtcaatct gcggcgattg cgacgtccat cacgcggcca
     5161 gcggcaacca acagccaaaa gcccgcgatg cctaacgatt tggtccgcat cgtggcgctt
     5221 gcaaatgccg gcgaagcgcg cgcacagatg attttggggc tgcacgacct cgacgacggc
     5281 aagggcggaa atcccgaaga ggcggcaaaa tggctcgaac gggccgccga gcagggcgaa
     5341 ccgatcgcgc aataccgcat ggcgacgatg tatgctgcgg ggcgcggtgt gaaagccgac
     5401 gacgccaagg cattccattg gtacgaggcc gcggcacagg ccggcaaccg aaaggcgatg
     5461 tccaatctcg cggtggccta cgcgcagggc aaaggggttg ggaaaaaccc gcaggaagcc
     5521 gtgcgctggt tctccaaagc agcggaactc ggcctggtcg acgcgcaatt cgatcttgcg
     5581 atcctctatg agcgcggtct tggcgttcgg caaagcctgt cgaatgccta tcgctggtac
     5641 gtgatcgcgg cgcgggctgg cgacaaggaa tccagggacc gcatcgacgc catcgcctcc
     5701 cagctttccg cgagcgatcg ggccaccggc gagaaatccg ccgccgagtt caaatcagcc
     5761 ccgatgaatc ccgaagcaaa cgagtcgaaa tagcggcgcg tggactccgg aacattgctc
     5821 tccataactc cgtcatggca gggccacccc gatccgcgcg acaagcaacc tcagtagccg
     5881 caacgcggga accacgctca gacagtcacg caacccgccg gattgcgtga taggcgacga
     5941 gcaccaccac ggcgccgatg attgcgacca gcatgctcca gagattgagg cccgaaacgc
     6001 cggtcgagcc gaaaaagcgg aatagccacc ctccgactat ggcgccgacg atacccagca
     6061 cgatatcgag gatgagccct tcccctgatt tgttgacgat cttgctcccg atgaacccgg
     6121 cgatcaggcc aagtatgatc cacgcaagaa tggacatcgg ttccctcccg ttttgatgcc
     6181 gtatcgatta ggctcccgg
//
LOCUS       DASZMC010000443        10290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4855521,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000443 DASZMC010000000
VERSION     DASZMC010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4855521"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..339)
                     /locus_tag="VG274_09155"
     CDS             complement(67..339)
                     /locus_tag="VG274_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06863.1"
                     /translation="MAELRGEVASEMRGPASGGAREHSWPSAAQLSSHGIRENSAQVL
                     PPGKPCETLLQKSGDAFREIFGRTGTLRIVRHPDIVTSDKRRAAAA"
     gene            338..2389
                     /locus_tag="VG274_09160"
     CDS             338..2389
                     /locus_tag="VG274_09160"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HWE06864.1"
                     /translation="MKRSLAALAVLFCTTAAAVAQLPAGHPQYGAWGIDLTAMDKSIK
                     PGDDFFEFVNGNWEKTAQIPPDRSSAGAFQDLRILSEKRMRAIVGGLDAKPYGELNDE
                     EKKLRDFYDAFMDQTQIESRGLAPVKKDLSTLARLKTLGDVAWAMGEVKLMTLAPFNL
                     NIDVDPKNPNAYAIFITQSGLGMPNRDYYLRDDKAIVETREAYKKYLAKMLGMAGLRD
                     AQKRADNVYAVELEIAKAQWPNEDRRDVDKTYNPMTVSALEQLAPQFSWKKFLGAAGV
                     SLASPHGERQVIVAEKSAFPKLAEIFAATPLSVWRDYLTVHYLHQAAPYLPKKFDDTD
                     FGFYGTVVQGNTQQLDRPTRGVHLLDNNLGEALGKLYVAKYFRPEAKAKAEQLVSNLL
                     KAYDADIRVLPWMTEATRQKALEKLHRFTPHIGYPEKWRDYSAFAVSRSALVEDVENG
                     TLFEWNRELKRIDGQVDRAEWGMSPPTINAYYTPVFNAIFFPAAILQPPFFDPNADDA
                     VNYGGIGAVIGHEISHGFDDQGSKYDGTGTLNNWWTAEDRKNFETRTKALTAQYNSYE
                     PLPGLHVNGAFTLGENIADLAGLTITYKAYHISLEGKPAPVLDGYTGDQRLFLSMGQI
                     WRSKYREGALRAQILSNEHAPAHFRPVGATRNIDAWYDAFDVKPGDKYYLPPDQRVHL
                     W"
     gene            complement(2423..2824)
                     /locus_tag="VG274_09165"
     CDS             complement(2423..2824)
                     /locus_tag="VG274_09165"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWE06865.1"
                     /translation="MTPAYRTFVAELFAEFGSVEVRRVFNFDGLYIEDTMFGLVADER
                     VFLKTDTESRKAFAAEDCGPLHYRAGDGADVEMSYYEMPSRLYDEPEEASAWARRAYG
                     IALRSPTALRKQRKPVKEKHARQPARSRSRS"
     gene            complement(2905..3300)
                     /locus_tag="VG274_09170"
     CDS             complement(2905..3300)
                     /locus_tag="VG274_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HWE06866.1"
                     /translation="MTVSTPIRARRERPKPIRLSDAAAARIRAIMEQAEGRYAGVRVG
                     VTNGGCAGMSYTMDYAEAAGPLDEVVEDRGVRIFIDPKAIMFLVGTEMDFVRDKLAAR
                     FVFNNPNQTAACGCGESVTLAPAKGEAAE"
     gene            complement(3303..3665)
                     /locus_tag="VG274_09175"
     CDS             complement(3303..3665)
                     /locus_tag="VG274_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HWE06867.1"
                     /translation="MSAEPLNMQAASTTLPPEELEAFTAKLVTALKTVYDPEIPVDIY
                     ELGLIYKVDVADNKDVAVEMTLTAPGCPVAGEMPGMVKDALVGVEGIGDVTVNMTFDP
                     PWTPEKMSDEAKLELNMY"
     gene            complement(3662..4120)
                     /locus_tag="VG274_09180"
     CDS             complement(3662..4120)
                     /locus_tag="VG274_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWE06868.1"
                     /translation="MDESLRELYQEVILDHSRHPRHFGALDGATHRGEGYNPLCGDRV
                     KVYLHVDGEGRIGDVSFEGRGCAISQASASLMTDLVKGRTLAESEKLMSGFLHLVKGD
                     GATELEGEDRERLDVMAGVSAFPMRVKCATLAWHTMKSALEEGGTTAKTE"
     gene            complement(4126..5370)
                     /locus_tag="VG274_09185"
     CDS             complement(4126..5370)
                     /locus_tag="VG274_09185"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWE06869.1"
                     /translation="MNLQLRRDDVFDVLSARKDFEILARQVYGKPLAYLDNAASAQKP
                     QTVLTAMTEFASTDYANVHRGVHYLSAKATQRYEAARETVRRFVNAAQADEIVFTKGG
                     TEAINLVAYSFLVPRIEPGDEIVLTTMEHHSNIVPWHFLRERHGSVLKWVDVNDDGSL
                     DPEAVDAAIGSRTKLLAVAHMSNVLGTVTPLREIVRRAQARGVPVLADGCQAAVHESV
                     DVQDLGVDFYALTGHKLYGPTGIGALYGKRVHLKSMRPFNGGGEMIAEVTRDNVLYAE
                     PPARFEAGTPPIVEAVGLAAALDYLTAHDRCLVRAHEQGLLDHATAEIEKLNWTRIIG
                     TARHKGAILSFEAAGMHAHDVATILDREGVAVRAGHHCAQPLMERFGVASTTRTSFAL
                     YNTHEEVDALIAGLHKARKILG"
     gene            complement(5367..6650)
                     /gene="sufD"
                     /locus_tag="VG274_09190"
     CDS             complement(5367..6650)
                     /gene="sufD"
                     /locus_tag="VG274_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HWE06870.1"
                     /translation="MNERATAEEFLLQDFAADAASRPALRPSWLDERRRRAVQSFRDA
                     GVPNRRIEQWKYTDLRGALEGQSATASSKPLLRPDRNPFAAISTFQLSIADGCLGEPN
                     SQIPAGIELFNLGQLDDGAPDWVRQNLGEVLSRSAMGEASLAMMRGGLAMRVSAATTI
                     PVHLGSPQKADSVHCRLLLVIEPGASLVLLESHGKSRGLINLGVEFVLEPNAELTHLR
                     LADSAPDAVQVEEIGIRVARDARYRGHFSQKGARLSRLELAIELEGEGAEAELSGASV
                     LGGKLHADVTTHIDHMAGRTASRQLFKNVAGGNSRAVYQGKITVRKGADGSDSRQTAK
                     AVLLGARAEADLKPELEILADDVKCAHGAAVGDLDADSLFYLRSRGLKEDEARRLLIH
                     GFLEEAIMEIDRDDVRLVVRDFLETGLAQALESGS"
     gene            complement(6647..7402)
                     /gene="sufC"
                     /locus_tag="VG274_09195"
     CDS             complement(6647..7402)
                     /gene="sufC"
                     /locus_tag="VG274_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWE06871.1"
                     /translation="MNMLEIENLHVAVQGQEILKGLSLAIRAGEVHAIMGPNGSGKST
                     LSYTLAGREGYEITEGRILYRGENLAPLTPEERAARGMFLALQYPVEIPGVTTMNFLK
                     TALNAQRRARGEKELDAMSVLKSVREAAGALNVPEEMLRRALNVGFSGGEKKRLEILQ
                     MALFRPVLAILDETDSGLDIDALKLVAGGVNALRAPDRGMLVITHYQRLLDYIVPDRV
                     HVLDKGRIVAEGGKELAHQLEAKGYAGLARDAA"
     gene            complement(7399..8850)
                     /gene="sufB"
                     /locus_tag="VG274_09200"
     CDS             complement(7399..8850)
                     /gene="sufB"
                     /locus_tag="VG274_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWE06872.1"
                     /translation="MAAIQETRERVASLGDRYKYGFITDIEMERAPRGLNEEVVRLIS
                     AKKGEPDWMLEWRLGAFRRWREMREPNWARVHYPKIDYENAYYYAAPKSSSNLASLDE
                     VDPKLLETYEKLGIPLHEQKLLAGVAVDAVFDSVSVATTFKAKLAESGVIFCPISEAI
                     REHGDLVRKYLGSVVPVTDNFFATLNSAVFSDGSFVYVPKGVRCPMELSTYFRINASE
                     TGQFERTLIIADEGAYVSYLEGCTAPKRDENQLHAAVVELVALDGAEIKYSTVQNWYP
                     GDEEGHGGIYNFVTKRGDCRGRASKISWTQVETGSAITWKYPSCILRGDDSVGEFYSI
                     AITNHFQQADTGTKMHHLGRNTRSRIISKGISAGRAQNTYRGLVSVHPRATNARNHTQ
                     CDSLLIGKECGAHTVPYIESRNPSAQIEHEATTSKIADDQLFYCLSRGIGQEEAVALI
                     VNGFCREVLQQLPMEFAVEAQKLVGISLEGSVG"
     gene            complement(8852..10003)
                     /locus_tag="VG274_09205"
     CDS             complement(8852..10003)
                     /locus_tag="VG274_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWE06873.1"
                     /translation="MPYLDHNATSTLRPEARAAMTRACEIHGNPSSVHRSGRAARALV
                     EDAREQVAQLVNANPANVIFTSGGTEANALALWGAVHGSAESSAPIERFYASAIEHDS
                     ILGTMMAITDRTAGVCAAEIPAMTDGLVDLDALHDMLRKSGGRALVAVMAANNETGVI
                     QPIAEVSKLVRKHGGLLLVDAVQACGKIATDFALDADYLTVSAHKLGGPQGVGVLAVK
                     EGAPFAAQIHGGGQERSRRAGTENVAGIAGFGAAAKAVGDDDVSRLEMLRNVFESGLR
                     NRFPAAIVFGQNSLRTANTSNFALPGIAAETALIALDLDGVMVSSGAACSSGRVKPSH
                     VLRAMAVPDNLARSALRMSLGWNSSESDVDAALASLEKLSTRIFARKAA"
     gene            complement(10013..>10290)
                     /locus_tag="VG274_09210"
     CDS             complement(10013..>10290)
                     /locus_tag="VG274_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWE06874.1"
                     /translation="YRLSRPASELRVADIILAVNEPISATRCKTGSPKGCTGTMSRCL
                     THDLWEELGRQIHVFLSSVSLADVVERRVLGRAHPRDPATPEAREAA"
BASE COUNT         1935 a         3325 c         3064 g         1966 t
ORIGIN      
        1 gacgcgcgct gattatccat aattgctcac ccttggagcg agttgactca gatcgctgca
       61 gggagattac gccgcggcgg cgcggcgctt gtcgctggtg acaatgtcgg ggtgcctcac
      121 gattcgcagc gtgccggttc ggccgaagat ttcgcggaag gcgtctccgc ttttctgcag
      181 aagcgtctcg cacggtttgc cgggcggtaa gacttgcgcg ctgttctcgc gaatgccgtg
      241 acttgaaagc tgcgctgcgc tgggccatga gtgctcccgc gccccgcccg acgcgggccc
      301 gcgcatctca gatgccactt ctccccggag ttcagccatg aaacgaagcc tcgccgcgct
      361 tgcggtcctt ttctgcacca cagcggccgc tgtcgcccag ctgccggccg gccatccgca
      421 atatggcgcc tggggcatcg acctcactgc gatggacaag agcatcaagc ctggcgacga
      481 cttcttcgag ttcgtgaatg gcaactggga gaaaaccgcc caaatcccgc ccgatcggtc
      541 ctcggccggt gcgtttcagg atctgcgcat cctcagcgaa aagcgcatgc gcgcgattgt
      601 cggcgggctc gacgcaaagc cctatggcga gctgaacgac gaagagaaga agctgcgcga
      661 tttctacgac gcgttcatgg accagacgca gatcgaatcc cgcgggctcg cgccggtaaa
      721 aaaggatctt tccactctcg cgcgactcaa gacgctgggc gatgtggcct gggcgatggg
      781 agaggtgaaa ctcatgacgc tggcgccctt caatctgaac atcgacgtcg atccgaagaa
      841 tcccaacgcc tacgcgatct tcatcactca gtcgggcctg ggaatgccca accgcgacta
      901 ctatctgcgc gacgacaaag ccatcgtcga aacgcgtgag gcgtacaaga aataccttgc
      961 caagatgctc ggaatggcgg ggctccgcga cgcccagaag cgcgccgaca atgtctacgc
     1021 ggtcgaactc gaaatcgcca aggcgcaatg gcccaacgaa gatcgccgtg acgtggacaa
     1081 gacctacaat cccatgaccg tttcggcgct cgagcagctc gcgccgcagt tttcatggaa
     1141 gaagtttctc ggcgcagcgg gcgtttccct cgcttcgccg cacggtgaaa ggcaggtcat
     1201 cgtcgccgag aaatcggctt tccccaagct cgccgagatc ttcgcggcga cccccctcag
     1261 cgtgtggcgg gactatctca ccgtgcatta tctgcaccag gccgcgccct atctgcccaa
     1321 gaaattcgac gacacggatt tcggattcta cggaacggtc gtgcagggta atacgcaaca
     1381 gctcgaccgg ccaacgcgcg gcgtgcatct tcttgacaac aatctgggcg aggcgctggg
     1441 caagctctac gtcgccaaat attttcggcc ggaggccaag gcgaaggcgg aacagctcgt
     1501 ctccaacctt ctcaaggcct atgacgcaga catccgcgtg ctgccatgga tgacggaggc
     1561 gacacgccag aaggcgctcg aaaagctgca ccggttcacg ccacacatcg gctatcccga
     1621 aaagtggcgc gattactccg cctttgcggt ttcgcgcagc gcgctggtcg aagacgtcga
     1681 gaacggcacc ctgttcgaat ggaatcgcga actcaagcgg atcgacggac aggtggaccg
     1741 tgcggaatgg ggcatgtcgc cgccgaccat caatgcctat tacaccccgg tgttcaacgc
     1801 gatcttcttt cccgccgcga tcctgcagcc gcccttcttt gatcccaatg ccgacgacgc
     1861 ggtcaattac ggcggcatcg gcgcggtgat cggccacgag atcagccacg gcttcgacga
     1921 ccagggctcg aaatatgacg gcaccgggac gctcaacaat tggtggacgg ccgaggaccg
     1981 caagaatttc gagacgcgca ccaaagcgct gacggcgcag tacaacagtt acgagccgtt
     2041 gcctggcctg cacgtgaacg gcgctttcac gctcggcgaa aacatcgccg atctggccgg
     2101 cctgacgatc acctacaagg cctatcatat ttcgcttgaa ggcaagcccg cgccggtgct
     2161 cgacggctac accggcgacc agaggctctt cctgtcgatg gggcagatct ggcgttcgaa
     2221 atatcgcgag ggtgcgttgc gggcgcagat actttccaac gaacacgcgc ccgcgcattt
     2281 ccgccccgtc ggtgccacgc gcaacatcga cgcctggtac gacgctttcg atgtgaagcc
     2341 aggtgacaaa tactacctgc cgcccgatca gcgcgtacac ctgtggtgaa gggcagcggt
     2401 gcgaaagagg gcgcagctta cctcacgacc gactccggct tctcgccggt tgccgcgcgt
     2461 gtttctcctt cacgggcttg cgttgcttgc gtaacgcggt gggcgatctg agcgcgatgc
     2521 cgtaagcccg ccgcgcccag gcagaagctt cttcgggctc gtcatagagc cgcgatggca
     2581 tctcgtaata ggacatctcg acatccgcgc cgtcgcccgc gcggtaatgc agcggcccgc
     2641 agtcttccgc cgcaaacgcc ttgcggcttt ccgtatcggt cttcagaaac acgcgctcgt
     2701 ccgcgacaag cccaaacatc gtatcttcga tgtacaatcc atcgaaattg aaaacacggc
     2761 gaacctcgac ggaaccgaat tccgcaaaca gttctgcgac gaatgttcga tacgccggcg
     2821 tcatggcagt ttcatatcca accaaatatg tgattgagtt gggtttgcga tacaatcgga
     2881 atcggcgctg aggttgtcta cggcctactc cgccgcttcg cccttcgccg gcgcgagcgt
     2941 taccgattcg ccgcagccac aggcggcggt ctggttgggg ttgttgaaca cgaagcgcgc
     3001 ggcgagcttg tcgcgcacga aatccatctc cgtgccgacg aggaacatga ttgcttttgg
     3061 atcgatgaag attcgcacgc cccggtcctc caccacttcg tcgaggggac cagcggcttc
     3121 cgcgtaatcc atggtgtagc tcatgccggc gcagccgcca ttggtcacgc ccacgcgtac
     3181 gcccgcatag cggccctccg cctgctccat gatggcgcgg atgcgcgcgg ctgctgcgtc
     3241 gctcagacga atgggctttg gcctctcgcg tcgcgcgcgg atgggggttg agaccgtcat
     3301 cctcaataca tgttcagttc gagcttggct tcgtcgctca ttttctcggg cgtccatggt
     3361 gggtcaaagg tcatgttgac agtgacatcc ccgatccctt ccacgccgac caaggcatcc
     3421 ttcaccatgc caggcatctc gccggccacc ggacagcccg gcgcggtcag ggtcatctcc
     3481 actgccacgt ccttgttgtc ggcaacatcg accttgtaga taaggccgag ttcgtagatg
     3541 tccaccggaa tctccggatc gtagacggtc ttgagcgccg tcacgagctt ggcggtgaat
     3601 gcttcgagct cctccggagg gagcgtggtg ctcgcagcct gcatgttcag cggttcggcg
     3661 ctcattcggt cttcgcggta gttccgccct cctccagcgc cgacttcatc gtgtgccagg
     3721 ccagcgtcgc gcatttgacg cgcatcggga aggcagaaac gcccgccatc acgtcgagcc
     3781 gctcgcgatc ctcgccttca agctccgtag cgccgtcgcc ctttaccagg tgcaaaaagc
     3841 cgctcatcag tttctcggac tctgcaagcg tgcggccctt gacgagatcc gtcatcagag
     3901 aggccgaagc ttgggagatg gcgcagccgc gcccttcgaa cgaaacgtcc ccgatgcggc
     3961 cctcgccgtc cacatgcaga tagactttca cgcgatcgcc gcagagcgga ttgtaaccct
     4021 cgccgcgatg tgttgcgccg tccagcgcgc cgaaatggcg cggatggcgg ctgtgatcga
     4081 ggatcacctc ctggtagagt tcgcgcaaag actcgtccat tgccgtcagc ccaaaatctt
     4141 ccgcgcctta tgcaaccctg cgatcagcgc gtccacttct tcatgcgtgt tgtataatgc
     4201 aaaggaggta cgggtcgtcg aggcgacgcc gaaacgttcc atcagcggtt gggcgcaatg
     4261 atggcccgct ctgaccgcga cgccttcccg atccaatatg gtggcgacat cgtgtgcgtg
     4321 catccccgcg gcttcgaatg acaggatggc gcccttatgt ctggccgtcc cgatgatgcg
     4381 cgtccagttg agcttctcga tttccgcggt cgcgtgatcc agcaagccct gctcatgcgc
     4441 acggaccaga cagcggtcgt gcgcagtcag ataatcgagc gcggcagcga gaccgacagc
     4501 ttccacaatg ggcggcgtgc ccgcctcgaa gcgcgcgggg ggctctgcat agagaacatt
     4561 gtcgcgcgtc acctcggcga tcatctcgcc gccgccgttg aacggccgca tggatttgag
     4621 atgcacgcgc ttgccgtaga gcgcgccgat cccggttggc ccgtaaagct tgtgaccggt
     4681 cagcgcatag aaatccacgc cgagatcctg cacatcgacg ctctcatgca cagcggcctg
     4741 acagccgtcc gcgagcacag gcacgcccct ggcctgcgcg cgccgcacga tctccctcag
     4801 tggcgtcacc gtaccgagca cgttcgacat gtgcgcaacg gcgagcagtt tcgtccggga
     4861 accgatcgcg gcatcgacgg cttcgggatc gagcgatccg tcgtcgttca catcgaccca
     4921 tttcagcacc gaaccgtggc gctcgcgcag gaagtgccat ggaacaatat tggaatggtg
     4981 ctccatcgtc gtgaggacga tttcgtcccc cggctcgatg cgcggaacaa gaaaggaata
     5041 ggccacgagg ttgatggctt cggtgccacc cttcgtaaac acgatctcgt ccgcttgcgc
     5101 ggcattgacg aagcgtcgca cagtttcccg cgcggcctcg tagcgctgcg ttgctttggc
     5161 ggagagatag tggacgccgc ggtggacatt cgcataatcg gtcgaggcaa attccgtcat
     5221 cgcggtcagc acggtttgcg gcttttgcgc cgacgcagcg ttgtcgagat aggcgagcgg
     5281 cttgccgtag acctgccgcg caaggatctc gaaatccttg cgggcggaga gcacatcgaa
     5341 cacatcgtcg cgacgcagct gaagattcat gagccggact ccagcgcctg cgccagcccg
     5401 gtttccagaa agtcccggac cacgagccgc acatcgtcgc gatcgatctc catgatcgct
     5461 tcctcgagaa agccgtgaat gagcaggcgg cgcgcctcgt cttcctttag gccgcgcgag
     5521 cgcaggtaga atagggaatc ggcatcgaga tcgccgaccg ccgcgccatg ggcgcatttc
     5581 acgtcgtcgg cgagaatctc gagttccggt ttcaaatccg cttcggcacg cgcgccgagc
     5641 agaaccgcct tcgccgtctg gcggctgtcc gatccgtcgg cgcctttgcg caccgtgatc
     5701 tttccctggt agacggcgcg cgaattcccg cccgcaacgt tcttgaacaa ttggcggctc
     5761 gccgtcctgc cggccatgtg gtcgatatgc gtcgtcacgt ctgcgtgcag tttcccgccg
     5821 agcacgcttg ccccgctcag ctcggcttct gcgccctcgc cctcgagctc gatcgccagc
     5881 tccagccgcg aaagcctggc gcccttttgc gaaaaatgcc cccggtagcg tgcatcccgc
     5941 gcaacccgaa tgccgatctc ctcgacctgc acggcatccg gtgccgaatc ggcgagccgc
     6001 aaatgggtca gttccgcgtt cggctcgagc acgaactcca ccccgagatt gatcaggccg
     6061 cggctttttc cgtgcgattc cagaaggacc agcgacgctc ccggctcgat cacgagcagc
     6121 agccggcaat gaacggaatc ggccttttgc ggagagccga gatgaaccgg aatcgtggtc
     6181 gcggcgctca cacgcatggc aagcccgccg cgcatcattg cgagcgacgc ctcgcccatg
     6241 gcgctgcgcg agagcacctc gccaagattc tgtctcaccc aatcgggcgc tccgtcgtcg
     6301 agttgtccaa ggttgaacag ctcgatgccc gccggaatct gcgaattcgg ttccccaaga
     6361 cagccgtctg cgatcgagag ctggaaggtt gaaatcgccg caaacggatt gcgatcggga
     6421 cgcaacaaag gttttgagga ggctgttgcg ctctgcccct ccagcgcgcc gcgcagatcg
     6481 gtgtatttcc attgctcgat tcggcgattg ggtacgcccg cgtcgcgaaa gctctgaacg
     6541 gcgcgccggc gccgttcatc gagccatgat ggccgcaaag ccggccggct cgccgcgtcg
     6601 gcggcaaaat cctgcagcag aaattcttct gcggtggcgc gctcgttcat gcggcgtcgc
     6661 gggccaggcc cgcatacccc ttcgcttcga gctggtgcgc cagttccttc ccaccctccg
     6721 cgacgatgcg gcccttgtcc agcacatgca cgcgatcggg aacgatgtag tcgagaagcc
     6781 gctggtaatg cgtgatcacc agcatgccgc ggtcgggcgc gcgcaacgca ttgaccccgc
     6841 ccgccaccag tttcagcgca tcgatgtcga gcccggaatc cgtctcgtcg agaatcgcga
     6901 gcacgggcct gaacagcgcc atctgcagga tttccagccg cttcttctcg ccgccggaga
     6961 agcctacatt gagggcgcgc ctgagcatct cttccgggac gttgagcgca cctgcagctt
     7021 cgcgcacgga tttcagcacg ctcatggcgt caagttcttt ctctccccgc gcgcggcgct
     7081 gggcattgag cgcggtcttg agaaaattca tcgtggtgac gcccggaatc tccaccggat
     7141 actgaagcgc cagaaacatg ccccgcgcgg cacgctcttc cggagtcagc ggcgcgagat
     7201 tctcgccccg atagagaatg cgcccctcgg tgatttcgta gccttcgcgg cccgcgagcg
     7261 tgtaggacag cgtcgacttg cccgatccgt tcgggcccat gatggcgtgc acttcgcccg
     7321 cccggatcgc gaggctaaga cccttcagaa tttcctggcc ctgaacagcg acgtgcagat
     7381 tctcgatttc gagcatgttc atcccacgct gccttccagg ctgatgccga caagcttctg
     7441 cgcttcgaca gcgaattcca tcggcaattg ctgcagcacc tcgcggcaaa agccgttgac
     7501 gatgagggcc accgcctcct cctgcccgat gccgcgcgac aggcaataga agagctgatc
     7561 gtcggcgatc ttcgatgtcg tcgcctcgtg ctcgatctga gcagaaggat tgcggctctc
     7621 gatgtagggc acggtgtggg cgccgcattc cttgccgatg agaagcgaat cgcattgcgt
     7681 gtggttgcgt gcatttgtgg cgcgcggatg cacgctcacg agcccgcgat aggtgttctg
     7741 cgcgcgccca gcgctgatgc ccttggagat gatccgcgag cgcgtgttgc ggccgagatg
     7801 atgcatcttg gtgccggtgt ccgcctgctg aaaatggttg gtgatggcga tggaatagaa
     7861 ctcgccgacg gaatcgtcgc cgcgaagaat gcagctcgga tatttccagg tgatcgccga
     7921 tcctgtctcg acctgcgtcc aggagatttt cgacgcgcgg ccgcggcagt caccgcgctt
     7981 ggtgacgaaa ttgtaaatgc cgccgtggcc ttcctcatcg cccggatacc agttctgcac
     8041 cgtggaatat ttgatctccg caccatccag cgccacgagc tcgactacag cggcgtgcag
     8101 ctggttctcg tcgcgcttcg gcgccgtgca gccttcgagg tagctcacat aggcgccctc
     8161 gtccgcgatg atgagcgtgc gctcaaactg gccggtctcc gacgcgttga tgcggaaata
     8221 ggtggaaagc tccatcgggc agcgcacacc cttcggcaca tagacgaacg acccgtcgga
     8281 aaacaccgcc gaattcagcg tcgcgaagaa attgtcggtg acgggaacca ccgagcccag
     8341 atatttgcgc accagatcgc cgtgctcgcg gatcgcttcg gagatcggac agaagatcac
     8401 gccggattcc gcaagcttcg ccttgaaagt tgtcgccacc gaaaccgaat cgaagacggc
     8461 gtcgacggcg acacctgcaa gaagcttctg ctcgtgtagc gggataccga gcttctcgta
     8521 ggtctcaagc agcttgggat cgacctcgtc gagactcgcg agattcgatg acgatttagg
     8581 cgctgcgtaa taataggcgt tctcgtagtc gattttcgga tagtgcaccc gcgcccaatt
     8641 cggctcgcgc atttcgcgcc agcgccgaaa ggcgccgagg cgccattcga gcatccaatc
     8701 cggctcgccc ttcttggccg agatgagacg caccacctct tcgttgaggc ccctgggcgc
     8761 gcgttccatc tcgatgtcgg taatgaaccc gtacttgtac ctgtcgccca gcgaggcgac
     8821 ccgttcccgt gtttcctgga tcgccgccat gtcaagctgc ctttcgcgcg aagatgcgcg
     8881 tagagagttt ctcgagagaa gcaagcgctg cgtcgacatc ggattccgag gaattccatc
     8941 cgaggctcat gcgcaacgca gatcgtgcca gattgtccgg cacagccatc gcccttagaa
     9001 catgcgatgg cttcaccctg ccagaagagc aggccgcacc cgaactcacc atcaccccgt
     9061 cgagatccag tgcaatgagc gcggtttccg cagcgatacc cggaagggca aagttcgacg
     9121 tattggctgt tcgcagagaa ttttgtccga acacgatggc ggccggaaag cggttccgga
     9181 gaccggattc aaacacattg cgcaacattt cgaggcgcga gacgtcgtcg tcaccaaccg
     9241 ccttcgcggc ggcgccgaag ccagcaattc cggccacgtt ttccgtcccg gcgcggcgac
     9301 ttctctcctg tccgccaccg tggatctgcg ctgcgaatgg cgcgccctcc ttcactgcca
     9361 acacgccgac gccctgcggt ccgccaagct tgtgcgcgga aacagtcaga tagtcggcgt
     9421 cgagggcgaa atccgttgcg atcttgccgc aagcctggac ggcgtccacg aggagcaacc
     9481 cgccgtgctt ccgcaccagt ttcgaaactt ctgcaatcgg ctgaatgact cccgtctcat
     9541 tgtttgccgc catcacggcg acgagcgcgc gtccgccgct cttccgcagc atgtcgtgca
     9601 aagcatcgag atcgaccagg ccgtccgtca tcgccggaat ttcggccgcg caaacgccgg
     9661 cggtacggtc cgtgatcgcc atcatcgtac ccaaaatcga atcgtgctcg attgccgacg
     9721 cataaaaccg ctcgatcgga gcacttgatt ccgcggagcc gtggaccgcg ccccagagcg
     9781 cgagcgcatt ggcttccgtt ccaccgctgg taaagatcac gttggccgga tttgcgttga
     9841 caagctgtgc gacctgctcc cgcgcgtctt cgaccagtgc gcgtgccgca cgtcccgagc
     9901 gatgcacaga cgatggattt ccatgaatct cacaggcgcg cgtcattgcg gcccgggcct
     9961 cggggcgaag cgtcgatgtg gcattgtgat caagataggg catctgcgca cctcacgcgg
    10021 cttcccgcgc ttccggtgtg gcggggtcgc gcggatgcgc gcgtccgagc acccggcgct
    10081 cgacgacgtc ggcgagagaa accgaagaca agaagacatg aatctgccgc ccgagttctt
    10141 cccacaaatc atgcgtgagg cagcgggaca ttgtgcctgt gcaccctttg gggctccctg
    10201 tcttgcagcg ggttgcgctg atcggctcgt tcaccgcgag aatgatgtcg gcaacgcgca
    10261 gttcgctggc gggccggctc aggcgatagc
//
LOCUS       DASZMC010000444         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2105412,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000444 DASZMC010000000
VERSION     DASZMC010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105412"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1836
                     /locus_tag="VG274_09215"
     CDS             67..1836
                     /locus_tag="VG274_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HWE06875.1"
                     /translation="MNAPASNLPPWPAMSLARAHALLTAPGARFEMEEVAIHGVRTRC
                     WKNAPPTLRDVLRMGRLFGDRDFLVYENERVTFEALWRASGAVAHALRANGLQKGDRV
                     AIVMRNLPEWAAIFYGAAVAGAIVTPLNAWWTGAELEYGLIDSGARFAFVDGERFERL
                     AEHMVNCPALERIFVSRSSDELLHPLAARLEDMIGCARDWIALPDRPLPDTPLAPDDD
                     VTILYTSGTTGKPKGALGTHRNMNSNIMAAGFSAARNFLRRGEPVPEIDPLTAPQRSA
                     LLSVPLFHATGCFAVLNPALCSGGKIVLMRKWEVEKALQLIEREKVNMAGGVPTIAWQ
                     LIEHPARAHYDLSSLESVAYGGAPSAPELVRMVAEAFPKSLPGNGWGMTETSATCTTH
                     SAEDYQHRPDSCGPAVAVCDLKVIAPDGSVLNRGEVGELWARGPNIVRGYWNRPQATA
                     ETFVDGWVRTGDLARLDEEGFCTIVDRAKDMLIRGGENIYCVEVENVLYEHPAVVDAA
                     LVAIPHHTLGEEPAAVVHLKAGLQATEEELRSFVAARLAAFKVPVKIVFWPESLPRNP
                     NGKILKSELKGQIGHLVSCAAAP"
     gene            2007..3260
                     /locus_tag="VG274_09220"
     CDS             2007..3260
                     /locus_tag="VG274_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWE06876.1"
                     /translation="MGSAACAAGVPYAPEALFATCILGVCLAFAMRAGRTGAGESGSA
                     LSDDSVDPVLLPPRARDLLERLPDPLMLLDQTSRVILANRAMVAVVGTDTRRKHVSAV
                     LRTPSVLQAIERTAATGEPASVEFSVRVPVEQHYEAYVTRTELGPRITAILLHDVTAA
                     LRAQEARADFVANASHELRTPLAALSGFIDTLRGHARNDEGARERFLDIMSVEAGRMS
                     RLIDDLLSLTRIEQNEHVPPLGEVDIMGVVCDAAAALQGLARVDEVTLCVAETDGLPP
                     IVGDRDELIQLFQNLMHNAIKYGRRGGHVSIALSPASAPVTPPGRHSAESFIAIAVKD
                     DGEGIPREFIPRLTERFYRVDVRRSRERGGTGLGLAIVKHIVNRHHGRLLIDSVAEEG
                     STFTVLLPAAPAPTREIAHAVTEMI"
     gene            3360..4076
                     /gene="phoU"
                     /locus_tag="VG274_09225"
     CDS             3360..4076
                     /gene="phoU"
                     /locus_tag="VG274_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWE06877.1"
                     /translation="MSEHTVRAFSEQLESLAAGVAQMGGLAEAQFTEAIEAIGKRDMS
                     LAEAAIAGDPRIDQLQQSIEERAVKVLALRQPMAIDLRDTLAAIKIASELERVGDLAK
                     NIAKRALVLNREPPIRLTSSLARMGKVTLGQLKLVLDSYSDRDAVGAEAVWRQDNEID
                     EIYNSLFRELLTYMMEDPRTIGLCTHLLFVAKNIERCGDHATNIAETVYHMVSGGHLA
                     SERPKADITSSTSVPFERKA"
BASE COUNT          773 a         1311 c         1378 g          761 t
ORIGIN      
        1 gccgccgttg cccggcagac gccatgtaat atgctgtgcc gttctcgata agggatcgcc
       61 tcgcatatga atgcaccggc ttcaaacctc ccgccctggc ccgcgatgtc tctcgcccgg
      121 gcgcacgcgc tgttgacggc gccgggagcg cgcttcgaga tggaagaggt cgcgatccac
      181 ggcgttcgga cgcgctgctg gaaaaatgct ccgccaactc tgcgcgacgt gcttcgaatg
      241 ggacgcctgt tcggcgatcg cgattttctc gtctacgaaa acgagcgcgt gaccttcgaa
      301 gcgctgtggc gcgcctcggg cgcagttgcg catgcgctgc gggccaatgg tctgcaaaag
      361 ggagaccgcg tggcaatcgt catgcgcaac cttccggagt gggcggcgat tttctacggc
      421 gcagcggttg ccggggcaat cgtgacacca ctcaacgcgt ggtggacggg cgcggagctc
      481 gaatacggcc tgatcgattc gggcgccagg ttcgcatttg tcgatggcga gcggtttgag
      541 cgacttgcgg agcatatggt caactgcccc gcgctggaac gaatcttcgt cagccgctca
      601 tcggacgagc tgctgcatcc gctggccgcg cggcttgaag acatgatcgg ttgcgcgcgt
      661 gactggattg cgctgccgga ccgtccgctg cccgacacgc cgctcgcgcc ggacgacgac
      721 gtgaccattc tctacacgtc ggggacaacg ggcaaaccga aaggtgcgct cggcacccac
      781 cgcaacatga attccaatat catggcggcc ggattttccg cagccagaaa cttccttcgc
      841 cggggcgagc ccgtgccgga gatcgatccc ctcacagcac cgcagcgttc ggcgcttctc
      901 tccgtgccgc tgtttcatgc gacaggctgt ttcgccgtgc tcaatcccgc gctttgctcg
      961 gggggcaaga tcgttttgat gcgcaaatgg gaggttgaaa aggccttgca gctcatcgag
     1021 cgggagaagg tgaatatggc gggcggcgtc cccacgatcg cctggcagct gatcgaacat
     1081 ccggcgcgag cgcactacga tctgtcgtct ctcgaaagtg tcgcctatgg cggcgcgcct
     1141 tccgcgccgg agctggtgcg catggtcgcc gaggcgtttc caaagtcgct tccgggcaat
     1201 ggctggggca tgacggagac atcggcgacc tgcaccaccc atagcgccga agattaccag
     1261 catcgtcccg attcgtgcgg tcccgcggtt gcggtgtgcg acctgaaagt gattgcgccg
     1321 gacggctcgg ttttgaatcg cggcgaagtg ggcgaattgt gggcccgcgg accgaatatc
     1381 gtgagaggtt actggaacag gccgcaggcc accgcagaga cttttgtcga cggctgggtg
     1441 cgaaccggcg atctcgcccg gctcgacgaa gagggcttct gcaccatcgt cgaccgcgcc
     1501 aaggacatgc tcatccgcgg cggcgaaaac atctactgcg ttgaggtcga aaacgtactc
     1561 tacgaacatc ctgccgtcgt tgacgcggca cttgtcgcca tcccccacca cacgctcggc
     1621 gaagagcccg ccgccgttgt gcacctgaaa gcgggcttgc aggcgacgga agaggaactg
     1681 cgcagctttg tcgcggcccg gctggctgcg ttcaaggtgc cggtgaaaat tgtcttctgg
     1741 cccgaatccc tgccgcgcaa tcccaacggc aagatcctca agtcggaact gaagggccag
     1801 atcggacacc tggtatcgtg cgcggcggcg ccatagccgc cccgaccatc ggggatgttg
     1861 cgcgtgaagt tttgatgacc aaggctgcgt tgctcccgat tgttgtatga tgcgccgggc
     1921 gattcaggtg gcgtggcgtg gtccgcagac ttcgtgaacg tgtagccatg cgcgaggggc
     1981 tatggtggct cagcggaggg gttgccgtgg ggtcggctgc gtgtgcagcc ggtgttccct
     2041 acgcgccgga agcgcttttc gccacgtgta ttctcggtgt gtgtctcgca tttgcgatga
     2101 gagcgggccg aaccggcgcc ggggaatccg gcagtgccct cagcgacgat tcggtcgatc
     2161 cggttctgct gccgccacgg gcgcgggact tgctggagcg ccttccggat ccgctcatgc
     2221 tgctcgacca aacaagccgc gtgatccttg ccaaccgggc gatggtcgcg gttgtcggca
     2281 ccgacaccag aagaaaacac gtgtccgcag ttctgcggac gccgtcggtg ctgcaggcca
     2341 tcgaacggac cgcggcgacg ggcgagcccg cgtcggttga gttttccgtc cgcgtgcccg
     2401 tcgaacaaca ttatgaagcc tatgttacgc gtacggagct tggtccccgc atcacggcca
     2461 ttctgcttca cgacgtgacc gccgcgctcc gcgcgcagga ggcgcgcgcc gatttcgtcg
     2521 ccaatgcgag ccacgagctt cgcacgccgc tggccgcgct atcgggtttc atcgacacgc
     2581 tgcgagggca cgccaggaat gatgagggag cgcgggaaag gtttctcgac atcatgagcg
     2641 tcgaagcggg ccgcatgagc aggctgatcg acgatttgct gtccctgacg cgcatcgaac
     2701 agaacgaaca cgtgccgccg cttggcgaag tcgacatcat gggggtcgtt tgcgacgccg
     2761 ccgccgcgct gcagggactg gcaagggtcg acgaggtgac tctctgtgtt gcggaaacag
     2821 atggtcttcc gccgatcgtc ggcgaccggg acgaactgat tcagcttttt caaaacctca
     2881 tgcacaatgc gatcaagtac ggccgccggg gcggtcatgt ctcgatcgcg ctatcgccgg
     2941 cctccgctcc cgtgacgccg cctggccgcc acagcgccga gtcgttcatt gcgatcgcgg
     3001 tcaaggatga cggcgagggc attccccgcg agttcattcc ccggctcacc gagcgtttct
     3061 accgcgtcga tgtccggcga agccgggagc gcggcggaac ggggcttggc ctcgccatcg
     3121 tcaagcacat cgtcaaccgt catcacggca ggctgctgat cgacagcgta gcggaagaag
     3181 gcagcacatt taccgtcctg ttgcccgccg cgccggcacc gacgcgcgaa atcgctcacg
     3241 ctgtcacgga aatgatataa tttcgtcaca taggcgtgtc ggatagtgga ttacgcaagc
     3301 ctatgttggg cgtgcgatgc gattcgggac cgaaagccgg agtggtgagc gaggaacgga
     3361 tgagcgagca cacggtaagg gctttcagcg agcagctcga gagccttgcg gccggggttg
     3421 cccagatggg cggcctggcc gaggcgcagt tcacggaggc gatcgaggcc atcggcaagc
     3481 gcgacatgtc tcttgcggag gcggccattg ccggggatcc gcgcatcgac cagctgcagc
     3541 aatcgatcga ggaacgggcg gtcaaagtgc tcgctttgcg ccagcccatg gcgatcgacc
     3601 tgcgcgacac gctcgcggcc atcaagatag cgagcgaact cgagcgcgtc ggcgatctcg
     3661 ccaagaacat cgccaagcgc gcgctggtgc tgaaccggga accccccatt cgcctcacat
     3721 cgagcctcgc ccgtatggga aaggtgacgc tgggacagct caagctcgtc ctcgattcct
     3781 attcggaccg cgatgcggtg ggcgccgagg cggtttggcg ccaggacaac gagatcgacg
     3841 aaatctacaa tagcctcttc cgcgagctgc ttacctacat gatggaggac ccgcgcacga
     3901 tcgggctgtg cacgcatctg cttttcgtcg ccaaaaacat cgagcgctgc ggcgatcacg
     3961 cgaccaacat cgccgaaacg gtctatcaca tggtcagcgg cggccatctc gccagcgagc
     4021 gcccaaaagc ggacatcacg tcctccacga gcgtgccctt cgagcgcaag gcatgatgct
     4081 gaaaccgact gtgctcgtcg ccgaagacga aggcgcgctc atcacgctgc tgcgctacaa
     4141 tctggagcgc gagggctacc gggtgatcga ggcgctgaac ggcgaggagg cgatacttgt
     4201 cgccaacgag cagcaacccg acc
//
LOCUS       DASZMC010000445         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_177053,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000445 DASZMC010000000
VERSION     DASZMC010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177053"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VG274_09230"
     CDS             complement(<1..468)
                     /locus_tag="VG274_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWE06878.1"
                     /translation="MKFTRLRLSGFKSFVEPTELFIEPGLTAVVGPNGCGKSNLFDAM
                     RWVMGETRPTSVRGSEMDDVIFAGSAGRPARNVAEVTIFVDNTDRTANAPYNEYDTIE
                     VSRRIEREAGSAYRINGRDVRQRDVQIFFADASSGASSTAFVRQGQIGLLVSQK"
     gene            complement(502..1182)
                     /locus_tag="VG274_09235"
     CDS             complement(502..1182)
                     /locus_tag="VG274_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWE06879.1"
                     /translation="MSRNQQIIAVVAVVIVLLAAAGYYLFFPASNTADAIPVSTDTGG
                     PGYTLTADDHMLGSPKAPITLIEYAAPTCPHCAHFNETVFPLIKAQYIDTGKVHYIFR
                     VYPLHPSDGAAEAMAECLPKDSYFQFIDLLFRNQPQWDWEYQVTDIHGGLVKMGRIAG
                     MTPEQVDKCIGDKAVQDHVNKVAQDGQTRYNISATPTFVINGYARTDVATWPDMQKAL
                     DALLAKKK"
     gene            complement(1252..1704)
                     /locus_tag="VG274_09240"
     CDS             complement(1252..1704)
                     /locus_tag="VG274_09240"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HWE06880.1"
                     /translation="MARRGRATPLGAEVNFAAHSAFVRAGFADPTLVLRWEEIAGSET
                     AALAQPVSLKDGPSGGVLTLKAEPGAALFLQHESRPLCERINAYLGRPAVARLRFVQG
                     ALIHRSKVVPRRRPPSAVSPEDPAQKYQGPEGLRAALLELARARKSDP"
     gene            1747..2838
                     /gene="mutY"
                     /locus_tag="VG274_09245"
     CDS             1747..2838
                     /gene="mutY"
                     /locus_tag="VG274_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWE06881.1"
                     /translation="MPPRRNPTQSPATRLLAWYDKHARVLPWRTPRGERADPYRVWLS
                     EIMLQQTTVQAVAAYYRKFLEQWPTVEALANAPLDDVLTAWAGLGYYARARNLHKAAK
                     VVAGELRGKFPSTMEGLRALPGVGAYTAGAIAAIAYDLREAAMDANAERVIARLFAVT
                     EPLAKAKPKLLVFGKSLVPQKRAGDFAQALMDLGSAICTPKRPACVSCPLFDDCVARK
                     QGIQETLPVKGQKAARPLKRGAAFVARDKSGAVLLIRRPESGLLGGMMQPPLGPWSED
                     FPSKAQAMKQAPFKADWKKRLDIVRHGFTHFELEIEVYETAVDKRPNVQGQWIGDLSK
                     AALPTVMRKIAVTPTDRLSRRRRTSRRSS"
     gene            complement(2783..3148)
                     /locus_tag="VG274_09250"
     CDS             complement(2783..3148)
                     /locus_tag="VG274_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06882.1"
                     /translation="MKGSALLLLSLTPLLASCSIFEVNKPLFGDENKVYQFFPLATDK
                     NFGKPILPTDKVKTEAQAVEIGRRSCGNEAADIAHWYANQSGDVWIVRWNVGQNSIYA
                     KVRKSDGTFDACEIDDPSG"
     gene            complement(3154..>3534)
                     /locus_tag="VG274_09255"
     CDS             complement(3154..>3534)
                     /locus_tag="VG274_09255"
                     /inference="COORDINATES: protein motif:HMM:NF037895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWE06883.1"
                     /translation="FIGIERESDYVKVARRRIAGVVPADSDAIEITKSKRAEPRIPFG
                     WVVERGLLPPGTMLQGTQKRHTAKVRADGTLVCSDATGSIHQIGAHVQGLDACNGWTF
                     WQYEDKGKLIPIDYLRQRLRADLV"
BASE COUNT          668 a         1039 c         1137 g          690 t
ORIGIN      
        1 cttctggctg accagcaggc cgatctggcc ctggcgcacg aacgcggtcg acgacgcgcc
       61 ggacgatgcg tccgcgaaga atatctgaac atcgcgctgg cgcacgtcgc ggccgttgat
      121 gcgataggcg gatcccgcct cgcgctcgat gcgacgcgag acctcgatcg tgtcgtattc
      181 gttgtaaggc gcgttggcgg tgcggtcggt gttatccaca aaaatcgtaa cttcggcgac
      241 gttgcgcgcg gggcgtccgg cgctgccggc gaagatcacg tcgtccattt cgctgccgcg
      301 cacggaggtc gggcgcgtct cgcccatcac ccagcgcatg gcgtcgaaca gattcgactt
      361 gccgcagccg ttcggaccca ccacggccgt gaggcccggc tcgatgaaca gctccgtcgg
      421 ttcgacgaag gatttgaaac cggaaagtct aagccgggtg aatttcaacg aacacgcgcc
      481 gccttgcgct gaaaacaaac gttatttttt cttggcgagg agcgcatcca gggccttctg
      541 catgtcgggc caggttgcaa catcggtccg cgcgtagccg ttgatgacga aagtcggcgt
      601 cgcggaaatg ttgtaccggg tctgtccatc ctgagcgacc ttgttaacgt ggtcctgtac
      661 cgccttgtcg ccaatacatt tgtcgacctg ctcaggagtc attccggcga ttcggcccat
      721 ctttacaagg ccgccatgga tatccgtcac ctgatattcc cagtcccatt gcggctgatt
      781 gcggaaaagc aggtcgatga actggaagta gctgtctttg ggcaggcatt ccgccatcgc
      841 ttcggccgcg ccgtcggaag ggtgcaaggg gtagacgcgg aagatgtagt gcactttgcc
      901 ggtgtcgatg tattgcgcct ttatgagcgg aaagacggtc tcgttgaaat gcgcgcaatg
      961 cggacaggtg ggcgccgcat attcgatcaa cgtgatcggg gccttcggcg agcccagcat
     1021 gtggtcgtcg gctgtgagcg tgtatccggg ccctccggtg tcggtcgata cggggatcgc
     1081 gtccgccgta ttgctcgcgg ggaagaagag gtagtagccg gctgccgcca gaaggacgat
     1141 tacaacggcg acgacggcga taatctgttg attccgcgac acggggctct cccaggaagt
     1201 tggacggcct gcttgtggca gagccgacgg cttccttcaa tgccggttgc gttaaggatc
     1261 ggactttcgc gctcgcgcaa gctctaaaag cgcggcgcga agtccttccg ggccttggta
     1321 tttttgcgcc ggatcctccg gagacactgc cgatggcgga cggcgccggg gaaccacctt
     1381 gcttctgtgg atcaacgcac cctgcacgaa tcgaagccgg gccacggcgg gtcggcccag
     1441 ataggcgttg atccgttcgc ataatgggcg actctcgtgc tgcaggaaca gggccgcgcc
     1501 gggttcggcc ttgagggtca gaacgccgcc gctcggtcca tcctttaggc tgaccggttg
     1561 ggcgagggca gcggtctcag aacctgcaat ctcctcccag cgcaaaacga gtgtcggatc
     1621 ggcgaagccc gcacgaacga acgccgaatg tgcggcgaag tttacctcgg cgccgagcgg
     1681 ggtcgcccgg ccgcggcggg ccatatccgg ttgtttgtct ttgggcgttt gcgccattgt
     1741 cggagtatgc cgccaagacg aaacccgacg caaagcccgg ccacgcgcct gctcgcctgg
     1801 tacgacaagc acgcgcgcgt cctgccgtgg cgtacgccaa gaggcgaacg cgccgacccg
     1861 tatcgcgtgt ggctgagcga gatcatgctg caacagacaa cggtgcaggc tgtcgctgcg
     1921 tactaccgga aattcctgga gcagtggcca acagtcgagg cgctagcaaa tgcgccgctg
     1981 gatgacgttc tcacggcgtg ggcagggctt ggctattacg cgcgcgcgcg caatctgcac
     2041 aaagcggcga aggtcgtcgc gggcgagttg cgcggaaaat tcccaagcac gatggaaggt
     2101 ctacgggctc tacccggtgt cggcgcctac acggcaggcg caattgccgc catcgcttac
     2161 gatcttcgcg aagcagcgat ggatgccaat gcggagcgcg tgatcgcgcg attgttcgcg
     2221 gtgaccgagc cgctcgcaaa ggcgaagccg aaactgctcg tattcggaaa atcgctcgtg
     2281 ccccaaaagc gcgccggcga ttttgcgcag gcgctgatgg acctcggctc cgcgatctgc
     2341 accccgaagc gtcccgcctg cgtgagttgc ccgctgttcg acgattgcgt ggcgcgtaag
     2401 caaggaattc aggaaacatt gccggtaaaa ggccagaaag ctgcgcgccc cctgaaacgt
     2461 ggcgcggcgt ttgtggcgcg cgacaaaagc ggcgcggtgc ttttgatacg acgcccggaa
     2521 agcggattgc tcggcggtat gatgcagccg ccgctcgggc catggagtga ggactttcca
     2581 tcaaaggcgc aggcgatgaa gcaggcaccg ttcaaagccg attggaagaa gcgcctcgac
     2641 atcgtgcgcc acggcttcac gcatttcgaa ctggagatcg aggtctatga aacggcggtg
     2701 gacaaaagac cgaatgtgca gggacaatgg atcggcgatc tttcaaaggc tgcgcttccg
     2761 acggtgatgc gcaagatcgc ggtcaccccg acggatcgtc tatctcgcag gcgtcgaacg
     2821 tcccgtcgct cttcctgact ttggcgtaga tcgagttctg gccgacattc cagcgcacga
     2881 tccagacatc gccagactgg tttgcatacc agtgcgcgat atcggcggct tcgtttccgc
     2941 agctgcgcct gccgatctcg accgcctgcg cttcggtctt caccttgtct gtgggcagga
     3001 tcggcttgcc gaaattcttg tcggtggcga gcggaaagaa ttgatagact ttgttctcgt
     3061 cgccaaagag cggcttgttg acttcaaata tgctgcagga cgccagtaac ggcgtcagcg
     3121 aaagcagaag gagcgcggag cctttcatgg ctttcaaacg agatcggcgc gcagacgctg
     3181 gcgcagatag tcgatcggaa tgagttttcc tttgtcttcg tactgccaga acgtccagcc
     3241 gttgcaggcg tcgagacctt gcacatgcgc gccgatctga tggatcgagc cggtcgcatc
     3301 ggaacagacg agcgtgccat cggcgcgaac cttcgcagtg tgccgcttct gtgttccctg
     3361 cagcatcgtg cccggcggga gcaatccgcg ctcgacgacc caaccgaaag gaatgcgcgg
     3421 ctcggcgcgt tttgatttcg tgatctcgat ggcgtcgcta tccgcaggca cgacgccggc
     3481 gatgcggcgg cgcgcgactt tcacgtaatc gctttcacgt tcgatgccga tgaa
//
LOCUS       DASZMC010000446         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_480741,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000446 DASZMC010000000
VERSION     DASZMC010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480741"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VG274_09260"
     CDS             <1..375
                     /locus_tag="VG274_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06884.1"
                     /translation="EISRFNAFQTSFGPELRRSDATLERMFQRLYGGRRGEAQYHAFK
                     TRLANHSSMRSIRSNYDYCREASLAFGAALASDKPTLVAFVKGVPVVEESPVNSCGLR
                     VAVGLAGVRALPDVTPRPRPER"
     gene            complement(629..1459)
                     /gene="trpA"
                     /locus_tag="VG274_09265"
     CDS             complement(629..1459)
                     /gene="trpA"
                     /locus_tag="VG274_09265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWE06885.1"
                     /translation="MSRIAGRFAELKRAGRAAFVPFVTAGDPDAETSFAILEQLPAAG
                     ADLIELGMPFSDPMADGPAIQASSQRALASGATLARVLDMVRRFRSRDNATPLVLMGY
                     YNPIHAFGTARFAKEASMAGVDGVIIVDLPPEEDEVLRLPAAAQSLDIVRLATPTTDE
                     RRLDVILNGAGGFVYYVSIAGVTGTKGFSTDEVRCAVARLKARTALPCAVGFGIKTAE
                     HAAEIACFADGAVVGSSIVDRVAQGIEKRLKRAAIVRDVTEFCATLAQSVHAAVRESL
                     "
     gene            complement(1695..2906)
                     /gene="trpB"
                     /locus_tag="VG274_09270"
     CDS             complement(1695..2906)
                     /gene="trpB"
                     /locus_tag="VG274_09270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWE06886.1"
                     /translation="MNIANSWRNGPDPTGHFGAYGGRFVAETLMPLVLRLEEAYEAAK
                     RDGGFRSELDGLLRHYVGRPSPLYFAERMTADLGGARIWLKREDLNHTGAHKINNCLG
                     QVLLARRMGKTRIIAETGAGQHGVATATVAARFGLPCVVYMGAVDMERQRPNVFRMRL
                     LGAEVREVTSGSKTLKDAMNEALRDWVANVDDTFYIIGSVAGPHPYPAMVRDFQTVIG
                     EETRSQILEAEKHLPDLLVACVGGGSNAIGLFYPFLDDDAVEIHGVEAAGAGTETARH
                     AATLGKGLPGVLHGARSYLLQTTEGQVVEAHSISAGLDYPGVGPEHSWLKDQGRVKYF
                     SATDREALEAFAALSRHEGIIPALEPAHALAHVMRVAPRMAKDSIIVMALSGRGDKDV
                     FTVAEAMGERV"
     gene            complement(2903..3565)
                     /locus_tag="VG274_09275"
     CDS             complement(2903..3565)
                     /locus_tag="VG274_09275"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002295.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWE06887.1"
                     /translation="MVQVKICGITRQEDSSAVLRAGADFAGFVFHRNSPRHVRPEQAA
                     ALAAPLRGRVRVVALVADAPDHEIAEAVCAADPDFIQLHGAESPERVAFIRVRFGKPV
                     IKALGIADETDFALLASYEDVADMLLFDAKPPMRESVPGGHGRSFDWQMIRTRTLRRP
                     WLLAGGLDAQNVSRAIRSSGAPGVDVSSGVETAPGVKSANAISDFIAAARASGLAGAV
                     QP"
BASE COUNT          689 a         1279 c         1244 g          645 t
ORIGIN      
        1 gagatttcgc gcttcaacgc ctttcagacc agtttcgggc ccgaactgcg taggtctgac
       61 gccaccctcg agcgcatgtt ccagcggctt tacggcggac gacgcgggga agcgcaatat
      121 cacgccttca agacccggct cgccaaccat tcttcgatgc gaagtatccg cagcaattac
      181 gattactgcc gcgaggcgag cctcgccttc ggcgcggccc tggcttccga caagcccacc
      241 ctggttgcgt tcgtcaaggg cgttccggtg gtggaggaat cacccgtcaa ttcctgcggg
      301 ctgcgcgtcg cagtcggact cgccggcgtc cgggccctcc ccgacgtaac gccgcggccg
      361 aggcccgagc ggtaggtagt aagagcgcgc actttcgaat ccgtccgcgc gcggacgcac
      421 gccccctgtt ttcctccccc aacgtaggga aggtggcccg aagggccggg agggggcgtg
      481 gttgcgcgcg cccccctccg cctcgctgag tttatcctcc ggccgcgctt cgcgcggacc
      541 ggggggctcg gcacctcccc cacgttgggg gaggaaagtc atgcggttgg cgtacatttc
      601 gcagctctgg aatatgacac ccattactct acaacgattc ccgaacggcg gcatgcacgg
      661 attgcgcgag cgttgcgcag aattcggtga cgtcgcgaac gatcgccgcg cgcttcagcc
      721 gcttctcgat gccctgggca acgcggtcga cgatggacga tcccaccacc gcgccgtcgg
      781 cgaagcaggc gatctcggcg gcgtgttccg ccgtcttgat tccgaaaccg accgcgcagg
      841 gaagtgcggt gcgcgccttg agccgggcca ccgcgcaccg cacctcgtcg gtcgagaaac
      901 ccttcgttcc cgtcacgccg gcaatcgaca catagtagac gaatccgccc gcgccattca
      961 ggatgacgtc gagtcggcgc tcgtccgtgg tgggcgtggc gagccgcacg atgtcaagcg
     1021 actgcgccgc ggcaggcagg cgcagcacct cgtcttcctc cggcggcaga tcgacgatga
     1081 taacgccgtc gacgcccgcc atcgacgctt ccttggcaaa gcgcgccgtg ccgaatgcgt
     1141 ggatgggatt gtagtagccc atcaacacca gcggcgtcgc gttgtccctg ctgcggaagc
     1201 ggcggaccat gtcgagcacc cgcgcgagcg tcgcgccgga cgcaagcgcc cgctgcgacg
     1261 aggcctggat cgcgggcccg tcggccatcg gatcggagaa cggcatgccc aattcgatca
     1321 gatcggcgcc tgccgcggga agctgttcga gaatggcgaa gctcgtctcc gcatccggat
     1381 cgcccgcggt gacgaacgga acgaacgccg cgcggcccgc gcgcttgagt tcggcgaagc
     1441 gtcccgcgat gcggctcacg agagcttctc tttcctcgaa ggcgccccac agccgcaccc
     1501 tgggacaagc ctacgcggag acgcggaagc gcggcgaaga aaggtcgaat cgccaaagcg
     1561 ccagggcgcc aggagtctct tgcgcgcatt cgcgtgcgat tcaacctggc gacttggcgc
     1621 cttggcggtt ttcatctccg tgcgcaaatg cgaatgccct gcctatagac ggggaagaga
     1681 gaatgcgtcc gagatcatac gcgctcgccc atcgcctcag ccacagtgaa cacatccttg
     1741 tcgccgcgtc cggagagcgc catgacgatg atggaatcct tcgccattcg cggcgcgacg
     1801 cgcatcacat gggcgagcgc gtgtgcgggt tcgagtgcgg gaatgatgcc ttcgtggcgc
     1861 gacagcgccg caaacgcctc gagcgcctcg cgatcggtgg cggagaagta tttcacccgg
     1921 ccctgatcct tgagccacga atgctcggga ccgacgccgg gatagtcgag gccggccgat
     1981 atcgaatgcg cttccacaac ctggccctcc gtcgtctgca gcagatagga gcgcgcgccg
     2041 tgcagcacgc cggggagacc cttgcccaat gtcgccgcgt ggcgcgcagt ttccgtgccg
     2101 gcgccggccg cctcgacgcc gtgaatctcg accgcgtcgt cgtcgagaaa gggataaaac
     2161 agtccgatcg cattcgagcc gccgcccacg caggcgacga gaagatcggg cagatgtttt
     2221 tcggcttcga gaatctgtga ccgcgtctcc tcgccgatga cagtctgaaa atcgcgcacc
     2281 atggcgggat aggggtgcgg gccggccacc gaaccgatga tgtagaacgt gtcgtcaaca
     2341 ttggcgaccc agtcgcgcaa cgcttcgttc atcgcgtcct tgagcgtctt cgagcccgac
     2401 gtgacctcgc ggacttcggc gcccagaagc cgcatgcgga acacgttggg cctctgccgc
     2461 tccatgtcga cggcgcccat ataaacgacg caaggaagcc cgaagcgcgc ggcgaccgtc
     2521 gccgtcgcca caccatgctg gccggcgccc gtctcggcga taatcctggt cttgcccatg
     2581 cggcgcgcga gcagcacctg gcccaggcaa ttgttgatct tgtgtgcgcc ggtgtgattg
     2641 agatcttcgc gcttcagcca gatgcgcgcg ccgccgagat ctgcggtcat gcgttcggcg
     2701 aaatagagcg gactggggcg ccccacatag tggcgcagca atccgtcgag ctccgacctg
     2761 aatccgccgt cgcgtttcgc cgcctcgtag gcctcttcga gccggagcac gagtggcatc
     2821 agcgtctcgg cgacgaagcg gccgccatag gcgccgaaat ggcccgtcgg atccggaccg
     2881 ttgcgccagg aattggcgat attcatggct gcacggctcc ggcgagcccg cttgcacgcg
     2941 cggcggcgat gaaatcggag atcgcgttcg ccgatttcac gccgggcgcg gtctccaccc
     3001 cggacgacac gtcgacgcca ggtgcgccgg aactgcggat cgcgcgcgac acgttctgcg
     3061 catcgagtcc gcccgcgagc agccacggcc tgcgtaatgt tcgtgtacgg atcatctgcc
     3121 aatcgaagga acgcccatga cctcctggaa cactttcgcg catcggcggc ttcgcatcga
     3181 agagcaacat gtcggcgacg tcttcgtagg acgcgagcag cgcaaaatcg gtttcgtcgg
     3241 cgatccccag agcctttatc accggcttgc cgaagcgcac gcggatgaag gcaacgcgct
     3301 cgggcgactc tgcgccgtgc agctggatga aatcgggatc ggcagcgcaa acggcttcgg
     3361 cgatttcgtg gtccggtgcg tccgccacca aggcgaccac gcgcacgcgc ccgcgcaacg
     3421 gcgcggcaag ggccgctgcc tgttccggcc gtacgtgccg tggcgaattg cggtgaaaaa
     3481 cgaaccccgc gaaatcggct cccgcgcgca gaacggcgct cgaatcctct tgccgcgtga
     3541 tgccacaaat tttgacttgc accatcgggc gccgcttatc cgcccccacg gtcgagaagg
     3601 caagtgggcc gaatgggttg caatgcctgg gcgcccgcgg cgggcgtcaa ggccaagagg
     3661 aaggaaattc tacaacgaga cagtgagatg gtgagggaag ataggcgtgt cggaggcgcg
     3721 caatgaaatt ctcaacgtct cactctgaat ccttttttgc cggcgggaca aacggagaag
     3781 tcactcctgc ccgccattgt tcaggcgggc gcgcagttcc ttgccggtgc ggaagacagg
     3841 ggcccgcttt tcatcga
//
LOCUS       DASZMC010000447         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4033574,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000447 DASZMC010000000
VERSION     DASZMC010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4033574"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..853
                     /locus_tag="VG274_09280"
     CDS             263..853
                     /locus_tag="VG274_09280"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWE06888.1"
                     /translation="MTASARPGSGLEKRKQKRAEIRLKGKLFVPQRGCEDDCTVIDFS
                     TDGAGVKCVGSAPIGTKVVLYIECFGRFEGMVVRRDRIRIGVEFQSSKVKRERTRAQL
                     SDFVANGMAVRAPIRTGTRAREMPPLHYFIAADGRKINCEIVDIALGGASLKTTERPR
                     VGEVLAFGETAGRVLRHTDEGIAVQFVGRRAADCAA"
     gene            993..1862
                     /locus_tag="VG274_09285"
     CDS             993..1862
                     /locus_tag="VG274_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWE06889.1"
                     /translation="MKLVTYVAGAGPRLGAVVGDSVVDVARHISGMPADMIALIRHWS
                     SARPRLEQALAGRPDRRLSDVRLRAPVARPGKILAIGLNYLDHIEETGAQRPLYPTFF
                     AKMSTAANGPFDSIELPAVSDELDYEAELVVVIGRRCRNVPRARAGEVVFGYCVGNDV
                     SVRDWQKRTGQWTLGKSFDTHAPFGPWITTADEIDPRALGVRSFVNGEPRQNSNTKQM
                     LFDCAAQIEHLSQAMTLEPGDLIFTGTPSGVGMARNPPLYLKTGDVVRVEIDGLGAIE
                     NRVEAASAASRFE"
     gene            complement(1875..2147)
                     /locus_tag="VG274_09290"
     CDS             complement(1875..2147)
                     /locus_tag="VG274_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06890.1"
                     /translation="MKRTKRASAKKTRSPAVATRRAPKEVLGEGNYTAARRFRQGETR
                     FVQRHADKSAELGQAAEPALGGEDRNVLEDAAARAAAHSHSPGEDV"
     gene            complement(2336..>2648)
                     /locus_tag="VG274_09295"
     CDS             complement(2336..>2648)
                     /locus_tag="VG274_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HWE06891.1"
                     /translation="LLPDIDLEWADVAVGAVGTAILYELGQLLIGLYLHSAGASAAYS
                     IAGGLIVVLLWIYYSAQVFLLGAELTKIYATRRGSRQGAGKTLAPRALENRGNRRLRG
                     A"
BASE COUNT          483 a          814 c          807 g          544 t
ORIGIN      
        1 aacgtacttc tttaccgtaa tctctttcga cgacatgatg accgtcccgg cgcagctcat
       61 tgcccgggaa gtgaatcacc catcgcccga aaagtgaatc acaaattcca ttcaaattga
      121 ttgacgcggg cgactagccc gagccggatg caaaacggac aatccgtcgg tttccccaat
      181 ccggtctttc gcaacgggat aaccagccgt taatttcgtt gcgccattct gttgcgcatt
      241 tcctgggttg gttggaaaaa gcatgacagc gtcggcacga ccgggtagcg gcctggagaa
      301 gcgcaaacaa aagcgcgccg aaatacgatt gaaaggtaag ctgttcgttc cgcagcgcgg
      361 ctgcgaggat gattgcaccg tcatagattt ttcgactgac ggcgctggcg tcaaatgtgt
      421 cggctccgcg ccgatcggca ccaaagtcgt gctctacatc gaatgtttcg gacgtttcga
      481 aggcatggtt gtccggcgcg accggatccg gatcggtgtg gaattccagt cgagcaaggt
      541 gaagcgtgag cgcacgcggg cgcagctttc cgatttcgtc gccaacggga tggcagttcg
      601 cgcacccatc agaacgggga cacgcgcacg ggaaatgccg ccgctgcatt atttcattgc
      661 cgccgatggt agaaaaatca attgcgagat cgtcgacatt gcgcttggcg gcgcttctct
      721 gaagaccacg gaacggcctc gcgtcggcga agtactcgcc tttggcgaaa cggcgggacg
      781 cgtcttgcgc cacacggacg aaggcattgc cgtgcagttc gtcggccgcc gcgccgccga
      841 ttgcgccgcc tgactgttgc ttccgacaaa gtcgaattgc gcggctgcac tcgcgcatca
      901 taaaagctgt tggtgcgttc tgtaacatgt gacatagccg gctacctggg caagctcggc
      961 agcgcggccc cccgctattg gaggcatcga tcatgaagct ggttacctat gtagctggcg
     1021 ccggaccgcg tctcggcgcg gtcgtcggcg acagcgtcgt cgatgtcgcc agacacatct
     1081 cgggcatgcc ggccgatatg attgcactta ttcggcactg gagttccgcc aggccgcgcc
     1141 tcgagcaggc gcttgccgga cgtcccgacc ggcggctttc cgacgtcaga ctccgtgcgc
     1201 ccgtcgctcg accgggcaag attctcgcca tcgggctcaa ttatctcgac cacatagaag
     1261 agacaggcgc acagcggcct ctgtatccta ctttttttgc aaaaatgtcc acggctgcca
     1321 acggtccgtt cgactcgatc gagcttccag cggtgtccga tgagctcgac tacgaagccg
     1381 agctcgtcgt ggtcatcggc aggcgctgtc gcaacgtgcc gcgcgcccgt gccggagaag
     1441 tcgtatttgg atattgcgtc ggcaacgatg tcagcgtgcg cgattggcag aagcgcacgg
     1501 gtcagtggac gctcggcaaa tcattcgaca cccacgcgcc cttcggaccc tggatcacga
     1561 cggcggacga gatcgatccg cgcgcgcttg gcgttcgcag tttcgtcaat ggcgaaccgc
     1621 gacagaactc caacacgaag cagatgttgt tcgactgcgc ggcgcaaatc gaacatttga
     1681 gccaggcgat gacgctggag ccgggcgatc tcattttcac cggtacgccg tcgggcgtgg
     1741 gcatggctcg caatccgccg ctctatctga agacaggcga cgttgtccgc gtcgagatcg
     1801 acggtctcgg cgcgattgaa aaccgggtgg aagcggcatc cgccgcatcg agattcgaat
     1861 agctcagggc gtttctaaac gtcctcgccg ggcgaatggc tgtgcgcggc ggcgcgcgca
     1921 gcggcatcct cgagcacgtt ccggtcctcg ccgccgagtg ctggttccgc cgcctgaccg
     1981 agttcggcgc tcttgtccgc gtggcgctgg acgaaccttg tctcgccctg acggaatctc
     2041 cgggcggcgg tgtaattgcc ttccccgaga acttccttgg gagcgcgtcg cgttgccacg
     2101 gccggcgagc gcgttttctt ggcggaagcc ctcttcgttc ttttcaccgc ggctttcttc
     2161 cgcctggggg ccgcccgttt gcgcgcagct ttcgtcggcg atttagccgc tgcccgcgct
     2221 ctgatcttct ttgtcctggc gcgcgcggtg cgtgcaggtt tcgccgtttt tcttgccctg
     2281 cgctttttgg caagcttctt catcttgttc tcctcgcggg aaacctcgcg agacatcatg
     2341 ctccgcgcag tcggcgattg ccacgatttt cgagcgcccg gggcgcaaga gttttcccgg
     2401 cgccctgccg gctgccacga cgcgtggcgt aaattttcgt cagctctgct ccaagaagga
     2461 acacctgcgc ggaatagtag atccagagga gcaccacgat caggccgccg gcgatcgaat
     2521 aggccgccga agcgcccgcg ctgtgcagat aaaggccgat gagcaattgc ccgagttcgt
     2581 aaagaatggc ggtgcccacg gcgcccacag ccacgtctgc ccactccagg tcgatgtcgg
     2641 gaaggagc
//
LOCUS       DASZMC010000448         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1585907,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000448 DASZMC010000000
VERSION     DASZMC010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585907"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            771..>2503
                     /locus_tag="VG274_09300"
     CDS             771..>2503
                     /locus_tag="VG274_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWE06892.1"
                     /translation="MTASPTSDSPRDYRATVFLPSTSFAMKAGLPDAEPKLLAGWEKM
                     DLYGRIRAQAKGRPRFVLHDGPPYANGEIHSGTGLNKILKDFVVRSQGMLGRDAPYVP
                     GWDCHGLPIEWKIEERYRAEGKTKDDVPIAQLRSDCRAFATHWIGVQREQFKRLGCIG
                     EWDKPYTTMDFRAEAAIAGELHKFVRNKLLYRGFRPVMWSPVEKTALAEAEVEYREKT
                     SPAIYVKFPVVAVCESTQTQDGRWNSVTTTPEKMPELGLDNVSLVIWTTTPWTIPGNR
                     AIAYSNDVEYGLYEVTGVSEGSLAERGERLVLADVLADQTAQAAKISLARRADVNPRN
                     IASCSHPFYGRGYDFPVPLLPGEHVTADAGTGFVHTAPGHGEDDFELGREHGLEVPRT
                     VDEEGKFYPTVPLFGGKRILTAEGKDGDANGAVIKELIAANALLAKGSLRHQYPHSWR
                     SKAPVIFRATPQWFAAIDKPLAEFGGDTLRVRAMESIRATRWFPRQAENRMAAMVDTR
                     PDWVLSRQRAWGVPIAIFVHRESGEILVDPDVDARIVDAFAQEGADAWYNSPPRRFLG
                     DGRDPDHYEQV"
BASE COUNT          521 a          727 c          779 g          476 t
ORIGIN      
        1 aaacttgcca tttccaacga ttatcgaccc cgcggcgccc ccgccacgaa cgcaacgtca
       61 cgttccgaac tcgatcgcca cgatgcggtt gcggctgatc gcggcgctcg caaagcggtt
      121 gccacgatgt ccatgttgcg caagaaaaaa tcggcgagga cgctatgcgt tatgacgcag
      181 taagattaat ctattcccga acacgccatt gcggctaaaa tagattaagc gtcccggtat
      241 ttgtaatgcg tcctttgaca ccgagtcaga agaccggtca ttccagcgaa tatcctcaag
      301 ggcatctttc gcgcaattgc tgacggcctg gaatcctcag tcaaacgggc caagtacggc
      361 acggaaattc gagatgctgg aacgtcatgg tcagtcttcg agtcgctaaa gggtggggac
      421 tcagtacgcc tgtttactga cgcgaacggg gtgcggatgg agtccgttca tgaaacactc
      481 ggcggaggtg gtcttgagat gtccatcgac aaacgcacgg cggcaatttc aggcgtttat
      541 ttcgagaaat aaagcaaact gtaccactcg acccagtgcc gggggcgctg gacgggcgtt
      601 tgaggcgctg ttagaaggca tcctgtccca ttgcaggctt cgccatgaaa ttcctgggtc
      661 tcgggaaaat attgcgaatt cgccgcccgg cgccgcgccg gggggcatga cgctgccctc
      721 gcgcggatcg ccggctcgcc gcgtccgcaa ctcctgaagg cctcgcccat atgaccgcat
      781 cgcccacgtc cgactccccg cgcgactatc gcgcaacggt ttttctgccc tcgacctcct
      841 tcgccatgaa ggcgggactg cccgatgccg agccgaaact gctggcgggc tgggaaaaga
      901 tggatctgta cggccgtatc cgcgcgcagg cgaaaggacg cccgcggttc gtgctgcatg
      961 acgggccgcc ctatgcaaat ggcgagattc attccggcac cgggctcaac aagatcctga
     1021 aggatttcgt cgtgcgcagc cagggcatgc tcggccgcga tgcgccttac gtgccgggct
     1081 gggactgcca cggtctgccc atcgaatgga agattgagga gcgataccgc gccgaaggca
     1141 agacgaagga cgacgttccg atcgcgcaac tgagaagcga ttgccgcgca ttcgccacgc
     1201 attggatcgg cgtgcagcgt gagcagttca agcgattggg ctgcatcggc gaatgggaca
     1261 agccgtacac gacgatggat ttccgcgccg aggcggcgat cgccggcgag ctgcacaaat
     1321 tcgtccgcaa caagctcctt tatcggggct ttcgtccggt gatgtggtcg ccggtcgaga
     1381 aaacggcgct tgcggaagca gaggtcgagt accgcgagaa aacatcgccg gcgatttacg
     1441 tgaagtttcc ggtcgtagcg gtttgcgaga gcactcaaac gcaggacgga cggtggaaca
     1501 gcgtcacgac tacaccagag aaaatgccgg agctggggtt agataacgtc tctctcgtca
     1561 tttggacgac aacgccttgg acgattccgg gcaatcgtgc gattgcctac tccaacgatg
     1621 ttgaatacgg cctgtatgaa gtcacaggcg taagcgaagg cagtctggca gagcgaggcg
     1681 aacgcttggt gcttgccgat gtcttagctg accaaactgc gcaagctgcc aagatatcgt
     1741 tggctcggcg tgccgacgtc aacccgagga acatcgccag ctgttctcat ccgttctacg
     1801 ggagaggata cgattttccc gtcccgcttt tgcccggaga gcacgttact gccgatgctg
     1861 gcaccggctt tgtgcatacc gcgccgggcc acggcgaaga tgatttcgag ctcggccgcg
     1921 aacacggact cgaagttcct cgtactgtcg acgaagaggg gaaattctac cccactgttc
     1981 cactgttcgg cggcaagaga attcttacgg ccgaaggtaa ggacggggac gcgaacggcg
     2041 cggtgatcaa ggagctgatc gcggcgaatg ccctgctcgc caagggttcg ctccgccatc
     2101 aatatccgca tagttggcgg tcgaaagcgc cggtgatctt ccgcgccacg ccgcaatggt
     2161 ttgcggcgat cgacaagccc ttggccgagt tcggcggcga caccttgcgc gtgcgtgcga
     2221 tggaatcgat ccgggcgacg cgctggttcc caaggcaggc cgagaatcgc atggccgcga
     2281 tggtggacac acggcccgac tgggtgctat cgcgccagcg cgcctggggc gtccccatcg
     2341 cgatcttcgt gcacagggag agcggggaga ttctggtcga tcccgatgtc gacgcgcgca
     2401 tcgtcgatgc attcgcgcag gaaggcgcag atgcatggta caattcgccg ccgcgacgct
     2461 ttctcggcga cggccgcgat cccgatcatt atgagcaggt gag
//
LOCUS       DASZMC010000449         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2661148,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000449 DASZMC010000000
VERSION     DASZMC010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661148"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VG274_09305"
     CDS             <1..665
                     /locus_tag="VG274_09305"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWE06893.1"
                     /translation="QQANRFKDEIVPLPTKMKKTDKATGTESIVDYTVDRDECNRPDT
                     TIEGLRALKPVLMGGMKVKEGKYVTAGNASQFSDGAAACVVMDAKLAEKKNLTPLGIF
                     RGFAVAGCEPDEMGIGPVFAVPRLLDRHGLKPGDIDLWELNEAFASQVLYCMGRIGIP
                     HEILNVNGGAISIGHPYGMSGARMTGHILLEGRRRKAKLGVVTMCVGGGMGAAGLFEF
                     VH"
     gene            662..1357
                     /locus_tag="VG274_09310"
     CDS             662..1357
                     /locus_tag="VG274_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWE06894.1"
                     /translation="MKRICDATLRGLALGCALAVCAACAGGGLIAKAPAPAPVDPKMQ
                     MAALESRIFQLVQDERRRINPDAKPLALDSELMGVARERSEDMAEKNYFAHRSPQGQT
                     SASLIMDEDQDFQGLLGENLAAQHFPKQSGVDVESFARRFVDTWLASPAHRDNLAFTT
                     YDRSAVGAAVGGDTIYVTELFASDLGLPAHTHDPKKREVSEWKDAKTAAAKPAKPPAK
                     QAANPPGAAQTAP"
     gene            1357..1695
                     /locus_tag="VG274_09315"
     CDS             1357..1695
                     /locus_tag="VG274_09315"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HWE06895.1"
                     /translation="MSKPIRHYSEFWPYYLREHAKPQTRALHYLGTGLATAAMIGLAI
                     TGRLWLVPLVLVAGYGPAWVAHFFVEKNRPATFTYPLWSLVSDYRLAWTWATGRLGTE
                     LAKAGVNQHR"
     gene            2043..>3521
                     /locus_tag="VG274_09320"
     CDS             2043..>3521
                     /locus_tag="VG274_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06896.1"
                     /translation="MQSELPWNVAGIAPEAREAARVSARREGLSVGEWLTRHILSDLP
                     QEADTPSEASDAPDTIGSRSPSNKLLSAQASMERLARVARSQSESHDAGRRIEEHFMG
                     LERRLQQTERSQSENNRAIGRAAEEISIASRGQAQAFGKLGAHVVGLNERVARLEKRV
                     SADMSREAIKALHLGLSRLADQITETSAHSAAQAERLAASIESVAGNLSRSHSENQQS
                     SHALENRIEAFTAHVDTIAAGMAETRNDAEQYRRAVDERIAAMESRVHSAERAALAGV
                     DAIEKALVEIQVSHAARKTDEAEIHHHDAALTQLSESLDRLIQRFSVSENGHADAIAR
                     VEAEISRIETAGRTEAVDGHLHEIGQTLAAIGNRIEKIERENTSFSNGVLQDLRDLGV
                     RMDAADRRHAESPAAAAPAESELRATPENEPPAPPPNHSNAIARIDLPPRAEVLPSET
                     EAAPPNQTETSDPAPESAFSSPDVGEVPTPDQVRGSRMGASLA"
BASE COUNT          715 a         1130 c         1106 g          570 t
ORIGIN      
        1 cgcagcaggc gaacaggttc aaggacgaga tcgtgccgct gcccaccaaa atgaagaaaa
       61 ccgacaaggc gacgggcaca gaatcgatcg tcgattacac ggtcgatcgc gacgagtgca
      121 atcggccgga caccaccatc gagggacttc gggcgctcaa gcccgtgctc atgggcggca
      181 tgaaagtgaa ggaaggcaaa tacgtcaccg ccggcaatgc gtcgcagttt tccgacggtg
      241 cggcggcgtg cgtcgtcatg gatgcaaagc tcgccgagaa aaagaatctg acgccgctcg
      301 gaatttttcg cggtttcgcg gtcgcaggct gcgaacccga cgagatgggc atcggtcccg
      361 tgttcgccgt gcctcgcctg ctcgatcgtc acggtctcaa gcccggcgac atcgatctct
      421 gggaactgaa cgaagccttt gccagccagg tgctctactg catggggcgc atcggaatcc
      481 cgcacgaaat cctgaatgtt aacggagggg caatctcgat cggccatcct tacggcatgt
      541 cgggggcgcg catgaccggt cacattctcc tcgaaggacg ccggcgcaag gcgaaacttg
      601 gcgtcgtcac catgtgcgtc ggcggtggca tgggcgcggc cggcctgttc gaattcgtcc
      661 attgaaacgc atctgcgacg caaccttgcg cgggctcgcg ttgggctgcg cgcttgccgt
      721 gtgcgccgcc tgcgctggcg gcggattgat tgcaaaggcg ccagcgcccg ctcccgtcga
      781 ccccaagatg cagatggccg cgctcgagag ccgcatcttt cagctcgtgc aggacgaaag
      841 gcgcaggatc aatcccgacg caaaaccgct cgcgctcgat tccgagctga tgggtgtggc
      901 gcgcgaacgc agcgaagaca tggcggaaaa gaactatttc gcccacagga gtccgcaggg
      961 gcagacttcc gccagcctca tcatggacga agaccaggac ttccaagggc tgcttggcga
     1021 gaacctcgcc gcgcagcatt ttccgaagca gtccggcgtc gatgtcgaga gcttcgcgcg
     1081 gcgatttgtc gacacctggc tggcaagccc ggcgcatcgc gacaatctcg ctttcaccac
     1141 ctacgaccgc agcgccgtcg gcgccgcggt ggggggcgac acaatctacg tgaccgagct
     1201 ttttgcgagc gatctgggtt tgcccgcgca cacgcacgac ccaaagaagc gcgaagtcag
     1261 cgagtggaag gatgcgaaga cggcggccgc caagccggcg aagccgccag ccaaacaggc
     1321 tgccaatccg cccggcgcgg cgcaaactgc gccgtgatga gcaagccgat ccgccattac
     1381 agcgaattct ggccatacta tttgcgcgag cacgcgaagc cgcaaacgcg cgccctgcac
     1441 tatcttggaa ccggccttgc gacggccgcg atgatcggtc tcgcgatcac gggcaggctc
     1501 tggctggttc cgcttgtgct ggttgcgggc tacggcccgg catgggtcgc gcatttcttc
     1561 gtggagaaga accgcccagc cactttcacc tatcccctct ggtctctggt ctccgactac
     1621 cggttggcat ggacctgggc gaccggccgg ctcggaactg agcttgcgaa ggcgggcgtg
     1681 aaccaacatc gctagcggtt caatttggag agttaaaaaa agactgtcat ggccccactt
     1741 catggggcca cccagccagc gcgcgtccgc gcgctgaatg gacgctccga gcggtttcac
     1801 ctgggtggcc cgctcggagt ttacactccg accgcgctct cgcgcggatc ggggtggcgg
     1861 gccacgacaa atttgtggat acgatctgct ggaatcggac cacccggcgg ctgtcggcgc
     1921 cattcgtcaa ccgcgccagc acccgaaatc gtcaaatcta caactgcttg aagggttact
     1981 tctttacaca gcatgtgggc gccggtactt gtgcttgtaa gtgtggcgta tagcgattcg
     2041 aaatgcagtc tgagctcccg tggaatgtgg ccgggattgc gcccgaagcc cgcgaagcgg
     2101 cacgtgtttc ggcgcggcgg gagggcctct ccgtcgggga atggctgacc cgccacattc
     2161 tgagcgatct cccccaggag gctgacactc cgtccgaagc ctccgacgcg cccgacacga
     2221 tcggctcccg tagccccagc aacaagctgt tatcggcaca agccagcatg gaaaggctcg
     2281 cgcgcgttgc ccgcagccaa agcgaatcgc acgacgccgg acgccgcatc gaagagcact
     2341 tcatgggcct tgagcggcgg ctgcaacaga ccgagcgcag ccagtccgag aacaatcgcg
     2401 ccatcggccg cgcggcggag gaaatcagta tcgcgagccg cgggcaggcg caggcgttcg
     2461 gaaagctggg cgcgcatgtg gtcggcctca acgaacgtgt tgcgcggctc gagaagcgcg
     2521 tctccgcgga tatgtcgcgg gaagcgatca aggcgctgca cctgggcctc tcccggctcg
     2581 ccgatcagat caccgaaact tctgctcatt ccgcggcaca ggccgaacgg cttgcggcaa
     2641 gcatcgagtc cgtcgcggga aacctttccc gatcgcacag cgaaaatcag caaagctcgc
     2701 acgcgctcga aaaccgcatc gaagcgttca ctgctcacgt cgacaccatt gccgctggca
     2761 tggccgaaac gcgcaacgat gccgagcaat atcggcgcgc cgtcgatgaa cgcatcgcag
     2821 cgatggaaag ccgcgtgcac agtgcggaac gggccgccct tgccggcgtg gacgccatcg
     2881 aaaaggcgct ggttgaaatc caggtctcgc acgccgcgcg caaaaccgac gaggccgaaa
     2941 tccatcatca cgatgccgca ctgacgcagc tttccgagtc gctcgacaga ttgatccagc
     3001 ggttttccgt gagtgaaaac ggacacgccg acgcgatcgc gcgcgtcgag gctgaaattt
     3061 cgaggatcga aacggcaggc cgcaccgaag ctgtcgacgg gcatttgcat gaaatcgggc
     3121 agactctggc cgcaatcggc aaccggatcg aaaagatcga acgcgaaaat acgagctttt
     3181 cgaacggcgt tctgcaagac ctgcgcgatc tcggtgtgcg catggacgcc gccgacaggc
     3241 gccatgccga atccccggcg gcggcggcgc ccgcagagag cgaattgcgc gcgacgccgg
     3301 aaaacgaacc gcccgctccg ccgccaaacc actccaacgc aatcgctcgc atcgatcttc
     3361 cgccgcgtgc ggaggtcctg ccgtccgaaa ccgaggctgc gccgccaaac caaaccgaaa
     3421 cgtccgaccc cgcgcccgaa tctgcgtttt cctcccccga cgtgggggag gtgccgactc
     3481 cggatcaagt ccgggggagc cggatggggg cctcattggc t
//
LOCUS       DASZMC010000450        12096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5409861,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000450 DASZMC010000000
VERSION     DASZMC010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5409861"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VG274_09325"
     CDS             complement(<1..501)
                     /locus_tag="VG274_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06897.1"
                     /translation="MSAEHPGRILPRWEGAAPLDVVIAVMAFLAALALCASLVAGRTA
                     QNWRAGLSSGLTVQILPPTQGGAEAIDAKWRAETDAVLSILHETRGIMRVRLMSDAET
                     RALVEPWLGSGALVRELPLPRLVDATSLPGADVDVSQLGKQLRQAAPDALLDDHARWI
                     LRLRRAA"
     gene            complement(498..1208)
                     /gene="ftsE"
                     /locus_tag="VG274_09330"
     CDS             complement(498..1208)
                     /gene="ftsE"
                     /locus_tag="VG274_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWE06898.1"
                     /translation="MRARRYGALDRGAMLRFENVGMRFGAGAEILRDVTFLLEAGSFT
                     FLTGASGAGKTTLLKLMFLAEPPTRGLITLFGHDLATTRRSIYPALRRRIGVVFQDFR
                     LLDHLSAFDNVALPLRLAGRRAGDYARDVEELLAWVGLGEKLQAKPPTLSGGEQQRVA
                     IARAVVARPDLLLADEPTGNVDADIAQRLIRLFAELNRLGTTVLIATHDRSLLERTQA
                     REMRLVDARLVQLRAPRS"
     gene            1604..2026
                     /locus_tag="VG274_09335"
     CDS             1604..2026
                     /locus_tag="VG274_09335"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HWE06899.1"
                     /translation="MAGWSGLAAMIVVAGWTGLRFRQEVVSLWPQSSSLYELLGVAAS
                     AKGIAIVDPAFHRELEDGQPVLAVTGRLVNTSSREVGVPAIRVALFDRDERELCHWTI
                     SPSQPTLRPGQSVKFFTQLSSPPAGARHLEMRFAASRE"
     gene            2023..2556
                     /locus_tag="VG274_09340"
     CDS             2023..2556
                     /locus_tag="VG274_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWE06900.1"
                     /translation="MNDRLNLPVLFSQEQIGTRVAALAGEISAMSDLPDLALPVLVGG
                     FVFAADLLRALDERGVSLPVEFLRLSRYGDSRVPLREPRIVMGPGERVRGLHVLIIDG
                     VLDHGHTLDAARRIVLETGARAVTTAAVIDKRRSGAPLRADFAAFTGVPHFVVGYGMD
                     DAGSLRSLPYIAAVEQA"
     gene            2553..3224
                     /locus_tag="VG274_09345"
     CDS             2553..3224
                     /locus_tag="VG274_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWE06901.1"
                     /translation="MIEAVIWDFGGVFTSSPFEAFARYERERDLPGDFIRRINSINHL
                     DNAWARFERAELDLDGFDRAFAQEALALGHGVPGKDVIALLAGDVRPEMVEALEGVRK
                     RFKTGCITNNVPSNQTGSADGRALYVREIMELFDTVIESSKLGLRKPDPRIYEIMCEA
                     LNVSPRNCAYLDDLGINLKPARAMGMTTIKVERAAQAIAELEQVLGMKLRENGQAPEG
                     PHTRA"
     gene            complement(3366..3599)
                     /locus_tag="VG274_09350"
     CDS             complement(3366..3599)
                     /locus_tag="VG274_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06902.1"
                     /translation="MIHCKIRTDIVGTRIRPAPTEYEDRTTPEGKTSSEHWIRFAFKL
                     VAITRFRSGKGRVVLAKDFAWTSRRSVSPLTHA"
     gene            complement(3596..5758)
                     /gene="katG"
                     /locus_tag="VG274_09355"
     CDS             complement(3596..5758)
                     /gene="katG"
                     /locus_tag="VG274_09355"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HWE06903.1"
                     /translation="MDKESKCPVANSQGRRNREWWPDQLDIQVLHQNSNKSDPMGEPF
                     DYAREFKSLDLNAVIRDLHALMTESQDWWPADFGHYGGLFIRLAWHSAGTYRIADGRG
                     GAGAGQQRFAPLNSWPDNANLDKARRLLWPIKQKYGRKISWADLFVLVGNVALESMGF
                     KTFGFAGGRADSWEPEELYWGPEGTWLGDERYSGERELQNPLGAVQMGLIYVNPQGPN
                     GKPDPVAAARDIRETFARMAMNDEETVALIAGGHSFGKTHGAGDPSLIGPEPEGAAIE
                     DQGLGWKNKHGMGFGADAITGGPEVTWSQTPTKWSNHFFDNLFKYEWELEKSPAGAYQ
                     WKAKNAAADVPAAHDKAKKHVPTMLTTDLSLRLDPAYEKISRRFYEHPEQFADAFARA
                     WFKLTHRDMGPIQRYLGPLVPKETLIWQDPIPAVDHKLIDDKDIESLKAEILGSGLSV
                     SQLIATAWASASTFRGSDKRGGANGARVRLSPQKDWEVNQPKQLATVLSKLEAIQKEF
                     NASPGGGKKVSLADLIVLGGAAAIEKAAKDAGAHVKVPFTPGRMDASQSQTDAVSFAP
                     LEPLADGFRNYYRGKDLMAPEEALVDKAQLLRLTGPEMTVIVGGLRVLGANAGGSKHG
                     VFTRKPETLTNDFFVNLLDMRTEWQPTASDGVYEGRDRKTKDVKWTGTRVDLIFGSHS
                     QLRAFAEVYGSADAKDKFVNDFITAWNKVMNADRFDLA"
     gene            complement(5884..6360)
                     /locus_tag="VG274_09360"
     CDS             complement(5884..6360)
                     /locus_tag="VG274_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWE06904.1"
                     /translation="MRRACQPRKMESRIDVMGRPKRQFGQHAKSGARLRAAGLRPTRQ
                     RLRLAELLFRHGNRHVTAELLHREAQDVGCAVSLATVYNTLHQFTAAGLLQQVIVDPS
                     RSYFDTNTGAHQHFYDEEDGGLIDIAGEAVEVSGLPAPPKGKAIARVDVVVRIRRA"
     gene            6462..7004
                     /gene="fabA"
                     /locus_tag="VG274_09365"
     CDS             6462..7004
                     /gene="fabA"
                     /locus_tag="VG274_09365"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HWE06905.1"
                     /translation="MLGRKPSFDLQGLLACARGELFAEGPRLPLPPMLMFDRITRISE
                     HGGSQNKGEIEAELDVHPDLWFFKCHFQDDPVMPGCLGLDAMWQLVGFFLGWLGGLGR
                     GRALGVGEVKFSGMVLPGAKLVSYYIDLKRVIMRRLVVGVADGIMKVDGKVIYEAKDL
                     RVGLFTEQAQSALASAAVPA"
     gene            7047..8273
                     /gene="fabB"
                     /locus_tag="VG274_09370"
     CDS             7047..8273
                     /gene="fabB"
                     /locus_tag="VG274_09370"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HWE06906.1"
                     /translation="MRRVVVTGMGIVSSIGNNVQEVASSLRETRSGITLAEAYRDMGF
                     RCQVHGSIKVNLEERVDRRARRFMGEGAAYNFLAMEQALADSGLEEKDIVNPRTGLIM
                     GSGGPSTRAIVAAADTARSEPVKGPKRVGPFVVPKAMSSTCSANLSTWYKIKGVNYSI
                     SSACSTSAHCIGNAGELIQWGKQDIVFAGGGEELDWTLSVLFDAMGAMSSHFNDRPEK
                     ASRAFDRSRDGFVISGGGGVLVLEELEHAKARGAKIHAELVGYGATSDGHDMVAPSGE
                     GAVRCMRQALATVNGPIDYINAHGTSTPVGDATEIAALREVFGSRIPPVNSTKSLTGH
                     SLGAAGVQESIYSLLQMQGGFIAESANIDELDLEIADAPVVRSRIDGAKIERALSNSF
                     GFGGTNATLVFERYHG"
     gene            8295..9197
                     /locus_tag="VG274_09375"
     CDS             8295..9197
                     /locus_tag="VG274_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE06907.1"
                     /translation="MTNSRGLMGGRRGLVMGVANDHSIAWGIARALARQGADVAFTYQ
                     GEAQARRLKPLAASIGSRIVLPCDVEDDAQLDEAFAVLRSEWGALDFLVHAIAHSDKN
                     ELKGRYVDTSRANFLRSLSISCYSFTAVAQRAAHLMTPPAAGGHGGALLTLTYLGATR
                     VTPNYNVMGVAKAGLEASVRYLAADLGRDGVRVNAISAGPMRTLAGSVIGEARTVFRW
                     NRAHSPLKKNIELEHVGNAALYLLSDLSAGVTGEIHFVDAGYNIVGMPPSADLKGWIA
                     PENGAENEDQEEAAGDRQSVRVGV"
     gene            9261..9635
                     /locus_tag="VG274_09380"
     CDS             9261..9635
                     /locus_tag="VG274_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE06908.1"
                     /translation="MRLNQVTVPAHDLDASIAFYEKLGFRLIVKHGPYARFELPDGEA
                     TLSVHATDKFVPGDVHVYFECEDLDKRIAELKAKGIVFDSDPEDQRWLWREAWLRDPA
                     GVKLCLYVAGENRRYPPWRLKG"
     gene            9638..10039
                     /locus_tag="VG274_09385"
     CDS             9638..10039
                     /locus_tag="VG274_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06909.1"
                     /translation="MAGYSRTPLAKKLGIRDGATLCIIAAPAEYRDWLAPLPPGVSFL
                     ARASNCDLVHLFVTSLKALERDLPKARAAIKSDGAIWVSWHKKSSKIPTDVTEDVVRA
                     RALRTDLVDVKVCAVTEDWSGLKLVVRKHLR"
     gene            10477..>12096
                     /locus_tag="VG274_09390"
     CDS             10477..>12096
                     /locus_tag="VG274_09390"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HWE06910.1"
                     /translation="MNETSGGITLNFVNADVRDVAKAVLGDYLKLNYEIAANVTGTVT
                     IQTSQPLARAQVLPALEQALGLNGLALVHSNGIYEVLPIADAHKQVGTTAPPAVTKTQ
                     GYGMEVVHVRYVSATAMQKLLEPIAGTEGAIRIDPARNVLIIEGTAQQRQTLLEDIAL
                     FDQDWLAGMSFALFEPKYMDADELAKELSDLIGGTDSPIAGVVRFVPIERLNGVLAIS
                     PQQRYLDQLRAWVERFDRPGEGNDKKIYVYRVQNGRATDIAATLIKTLFGSSSTEPIQ
                     QPAQTGASRFNATGTTGTTGTSGTTGTSGTTGTNGTSGTGGPGGLGGNTGFGQNTGTG
                     GTSNTGVGGNSLTANRSQSPAVPFAPVTTPVEQQGARTGGRYETVGGVSRENISSVNI
                     TADESNNALVILATPREYKVISDALRELDIQPLQVLLEAAIAEVTLNKNTQYGFQYFY
                     QPNSTPNSLSTFTLTNAATLAAPPGSPLLAALPGFSYMFANGNSLQAVLSAIATETHV
                     EVLSAPEVLVLNNQTASLEVGDQVPIVTATSV"
BASE COUNT         2167 a         3864 c         3847 g         2218 t
ORIGIN      
        1 ggccgcgcgc ctgagccgca gaatccatcg cgcatgatcg tcaagcagtg cgtcgggcgc
       61 ggcctgccgc aattgcttgc cgagctgaga cacatctaca tcggcgccgg gcagcgaggt
      121 cgcgtccacc agacgcggca gcggcaattc cctcacgagg gcgccggagc ccagccacgg
      181 ctcgacgagg gcgcgcgtct cggcgtcgct catcagccgc acccgcatga tccctctcgt
      241 ttcatgcagg atagacagca ccgcatcggt ttccgcccgc cactttgcgt cgatggcctc
      301 cgcgccgcct tgcgtgggcg gaagaatctg cacggtcagt cccgacgaca gccccgcgcg
      361 ccagttttgc gcggtccgtc cggcgacaag cgaggcgcag agcgcgagag cggcgaggaa
      421 cgccatcacc gcgatcacca cgtcgagtgg cgccgcgcct tcccagcgcg gcaaaatccg
      481 gcccggatgc tcggcgctca tgaacggggc gccctcaatt gcacgagccg cgcgtccacg
      541 agacgcattt cccgcgcctg ggtgcgctcc agcagggagc gatcgtgggt tgcgatcagc
      601 acagtcgtgc ccagccggtt caattccgcg aacagcctga tgagcctctg cgcgatgtcg
      661 gcgtcgacat tgccggttgg ctcgtcggcc aggagaaggt cggggcgcgc gaccaccgcg
      721 cgggcaatcg cgactcgctg ctgttcgcca ccggaaagcg tgggcggttt ggcctggagc
      781 ttttcgccga ggcccaccca ggcgagcaac tcttcgacat cgcgggcgta atcgcccgcc
      841 cgccggcccg ccagacgcaa cggcagcgcg acattgtcga acgccgaaag atgatcgaga
      901 agccggaaat cctggaacac cactccgatg cgacggcgca gcgccgggta gatcgatcgc
      961 ctggtggtgg cgagatcatg accgaagagc gtgatgagac cccgcgtcgg cggctccgcc
     1021 aggaacatca gtttgagaag cgtggttttt cccgcgcccg aggctccggt caggaaggtg
     1081 aaagagcccg cttcgagcag aaaggtcacg tcgcgcagga tctcggcgcc tgcgccgaaa
     1141 cgcattccga cgttttcaaa ccgcagcatc gcgcctcgat cgagcgcccc atagcgccgt
     1201 gcccgcatcg cgaatatcgc agccgcgcgc acggctcaag tcttgcggcg cggaacgaat
     1261 cacagccgca tgtcagcaat atggtctgcg cttgcatcgt attggggcaa gtgcgtgaat
     1321 cgggatcatg atcctcgcct gcccagaatg ccggacccgc tatcagacgg atgacctgaa
     1381 ttttcccccc gccgggcgca aggtaagatg tgcaaagtgc gcccatgtct ggcaccagcg
     1441 ggcgccgcaa cgggaggtcg agctcacatc tgggcatgaa acttccgctt ctgccaccac
     1501 tgctcccctt gccgtcgcgt tggcaggatt cgccggcagg cccgccaccc ctttagcggc
     1561 cgcaaagcgc gcgcgtcgtt cactggcgga gaagcttggc atcgtggcgg gctggtccgg
     1621 cctcgccgcc atgattgttg ttgcgggctg gacgggattg cgcttccgcc aggaggtcgt
     1681 gtcgctgtgg ccccaatctt cgtcgcttta cgaactgctt ggggttgcag ccagcgcaaa
     1741 aggaatcgcc atcgtcgacc cggcttttca ccgcgaactc gaagacggcc agccggtgct
     1801 ggccgtgacg ggtcggctgg tcaacacgtc ctctcgcgaa gtgggcgtgc cggcgatccg
     1861 ggtggcgctc ttcgatcgtg atgaacgcga actctgccac tggacgatca gtccgtcgca
     1921 gcccacgctt cgccccgggc aatcggtgaa attcttcacc cagctgtcga gcccgcccgc
     1981 gggcgcgcgt cacctcgaga tgcgttttgc ggcgagccgg gaatgaatga ccggctcaat
     2041 cttccggtgc tgttttcaca agagcaaatc ggcacccgcg tcgcggcgct cgccggagaa
     2101 atttctgcga tgagcgacct gcccgatttg gcgttgcccg ttctcgtcgg cgggttcgtt
     2161 ttcgcggcgg acctgttgcg cgcgctcgac gagcgcggcg tctcgctgcc ggtcgaattt
     2221 ctgcggctgt cgaggtatgg cgattcccgc gtccccctgc gcgaaccccg cattgtcatg
     2281 ggccccggcg aacgcgttcg cggcctgcac gtgctcatca tcgacggcgt tctcgatcat
     2341 ggccacactc tcgatgccgc gcgccgcatc gtgctggaaa caggagcgcg ggcggtcacg
     2401 acagcggccg tgatcgacaa acggcggagc ggcgcgcctc tccgtgccga tttcgccgcc
     2461 ttcaccggcg tgccgcattt tgtcgtgggc tatggcatgg acgatgccgg tagtcttcgc
     2521 tcgctgccct acatcgccgc ggtcgaacaa gcatgatcga ggcggtgatc tgggatttcg
     2581 gcggcgtgtt cacgtcctcg cccttcgaag cgttcgcgcg ctacgagcgt gagcgagacc
     2641 tgcccggcga tttcatccgg cgcatcaatt ccatcaatca tctggacaat gcctgggcgc
     2701 gcttcgaacg cgcggaactc gatctggacg gattcgaccg ggcgttcgcg caggaggcgc
     2761 tggcgctggg gcatggcgtg ccggggaagg atgtgattgc gctcctggcc ggcgatgtca
     2821 ggccggaaat ggtcgaagcc ttggaaggcg tccgcaaacg cttcaagacc ggctgcatca
     2881 cgaacaacgt accttcaaac cagacaggat ccgcggacgg ccgcgcactg tatgtgcgcg
     2941 agatcatgga gctgttcgat accgtgatcg aatcctcgaa gctcggcctg cgcaagcccg
     3001 atccgcgaat ctatgagatt atgtgcgagg cgttgaacgt ctcgccccgc aattgcgctt
     3061 atctcgacga tctcggcatc aacctgaagc ccgcgcgcgc gatgggcatg acgacgatca
     3121 aggtcgagcg cgccgcgcag gcgattgcgg agctggagca ggtgctgggg atgaagctgc
     3181 gcgaaaatgg tcaggcgccc gaaggccccc acacccgcgc ctgaacggtt gatagctctc
     3241 gttctttagc cctctcccac aaggggagag gattccgcgc gctgatttac cgccgttgat
     3301 gcaggactcg tcgttcccct ctctccttgt gggagaggga tgcagatacg gacgataagt
     3361 tctgctcagg cgtgggtgag gggtgaaaca ctgcgcctgc ttgtccatgc gaaatccttc
     3421 gcaagcacca cgcggccttt gccgcttctg aagcgggtaa ttgcgacgag cttgaatgcg
     3481 aagcggatcc aatgctccga tgacgtcttg ccttcgggcg tagtgcgatc ctcgtattcg
     3541 gtcggcgccg gcctaatgcg cgttccgacg atatctgtac ggattttgca atggatcacg
     3601 cgaggtcgaa acgatccgca ttcatcacct tgttccacgc cgttatgaag tcgtttacaa
     3661 acttatcctt ggcatcggcg ctgccgtaga cttccgcgaa cgcgcgcaac tgcgaatgcg
     3721 aaccaaagat cagatcgacg cgcgtgccgg tccatttcac gtccttggtc ttccggtccc
     3781 ggccttcgta cacgccatca gacgcggtgg gctgccattc cgtgcgcatg tcgagcaggt
     3841 tgacgaagaa atcgttggtc agcgtctccg gcttcctggt gaagacgcca tgcttggacc
     3901 cgccggcatt ggcgcccaaa acgcgcagac caccgacaat caccgtcatc tccggcccgg
     3961 tcagccgcag caactgcgcc ttgtccacca gggcttcctc gggggccatc agatctttgc
     4021 cgcggtagta gttccggaag ccgtcggcga gcggttcgag cggtgcgaaa gatacggcgt
     4081 ctgtttgact ctgcgacgca tccatgcgcc cgggtgtgaa cggcaccttc acgtgggcgc
     4141 cggcatcctt tgcggccttt tcgatggccg ctgcgccgcc gagcacgatc aggtctgcga
     4201 gcgagacttt tttcccgccg cccggcgaag cgttgaactc cttttgaatc gcttcgagct
     4261 tcgagagcac cgtcgcgagc tgcttcggct ggttcacctc ccaatccttc tgggggctga
     4321 gacgaacgcg cgcgccattc gcgccgccgc gcttgtcaga gccgcgaaac gtcgacgccg
     4381 acgcccaagc ggtcgcgatt agctgtgata ccgacaatcc cgagccaagg atctccgcct
     4441 tcagggactc aatgtcctta tcgtcgatca gcttgtgatc cacggccgga attgggtcct
     4501 gccagatcag ggtctctttc gggacgagtg ggccgaggta gcgctgaatc ggtcccatgt
     4561 cgcgatgcgt aagcttgaac caggcgcgtg cgaacgcgtc agcgaattgc tctggatgct
     4621 cgtaaaaccg ccgcgagatc ttctcataag ccgggtccaa ccgcagcgag aggtccgtcg
     4681 tcagcatggt cggaacatgt ttcttcgcct tgtcgtgcgc agcaggcacg tcagcggcgg
     4741 catttttcgc cttccactga taggcgcccg ccgggctctt ctcgagttcc cattcatatt
     4801 tgaacagatt gtcgaaaaag tgattgctcc acttcgtcgg cgtctgcgac caggtgactt
     4861 ccgggccgcc cgtgatggcg tccgcgccga accccatgcc gtgcttgttc ttccagccaa
     4921 ggccctgatc ttcgatcgcg gcgccttccg gctctgggcc gatcagggac ggatcacccg
     4981 cgccgtgcgt ttttccgaag ctgtggccgc ctgcaatcag cgctacggtt tcttcgtcgt
     5041 tcatcgccat ccgcgcgaag gtctcgcgaa tatcgcgcgc ggctgcgacc gggtcgggct
     5101 tgccgtttgg tccttgcgga ttgacgtaga tgaggcccat ctgtaccgcg ccgagcgggt
     5161 tctgaagctc gcgttcgccg ctatagcgtt cgtcgccgag ccaggtgcct tccggtcccc
     5221 aatataattc ttccggctcc cacgaatcgg cgcggccgcc ggcgaaaccg aacgtcttga
     5281 agcccatcga ctccagtgcg acgttgccaa cgagaacgaa gagatcggcc caggaaatct
     5341 tgcggccata tttctgcttg atcggccaca acagacggcg tgccttgtcg aggttcgcat
     5401 tgtccggcca gctgttgagc ggcgcgaagc gttgctgtcc ggcgcctgcg ccgccgcgtc
     5461 catcggcaat gcgataggtg cccgcgctgt gccaggcgag gcgaatgaac aggccgccgt
     5521 agtggccgaa gtcggccggc caccaatctt gcgattccgt catcaacgca tgaagatccc
     5581 tgatcaccgc gttgaggtcg aggctcttga actccctggc gtagtcgaac ggctcgccca
     5641 tcgggtcgga tttgttggaa ttctgatgga ggacttgaat gtccagctga tctggccacc
     5701 attcgcgatt ccttctgccc tgcgaattgg cgacggggca cttgctttcc ttgtccatat
     5761 cctgctcccg ggagttttgc ggttgttcgc gggcaaaaag gtcatcgcgc ccacgagcag
     5821 ctcgaaagtt atcccccggc aacccggtcg tcaactagtt tggaataatt ccaaagaaga
     5881 atttcaggcg cgacggatgc gcaccaccac gtctaccctg gcgatcgcct tgccctttgg
     5941 aggcgccggc aggcccgaga cctccaccgc ttctccggcg atatcgatga gcccgccgtc
     6001 ttcctcgtcg tagaagtgct gatgggcacc ggtattcgta tcgaaatagc tgcgcgacgg
     6061 atcgacgatt acctgttgca gaaggccggc cgccgtgaac tggtgcagcg tgttgtagac
     6121 ggtggccagc gaaaccgcgc agcccacgtc ttgcgcctcc cgatggagga gttcggcagt
     6181 cacgtgccgg ttgccatggc ggaaaagcag ttctgcgagc cgcagccgct gccgtgtagg
     6241 gcgcaacccg gctgcgcgaa gcctggcgcc ggatttcgcg tgttgcccaa actgcctttt
     6301 tggtcgtccc atgacgtcga ttcggctttc catcttgcgc ggctgacacg cgcgcctcat
     6361 ttttgctagg ctgggattct ggccactgat tagaatattt ctaatccgca ggaaatcgcc
     6421 aacaggcccg ctctgtcaag ggccgcacag gaacgaacct catgctggga cgcaaaccga
     6481 gcttcgactt gcagggcttg ctcgcctgcg cgcgaggcga gcttttcgcc gaaggccccc
     6541 gcctgcctct gccgcccatg ctgatgtttg accgcatcac caggatttcc gagcatggcg
     6601 gaagccagaa caagggcgaa atcgaagccg agctcgacgt gcaccccgac ctctggttct
     6661 tcaaatgcca tttccaggac gatccggtca tgccgggctg cctggggctc gatgcgatgt
     6721 ggcagctcgt cgggttcttt ctgggctggc tcggcggatt ggggcgtggg cgcgccttgg
     6781 gcgttggcga agtcaaattt tcgggcatgg tgctgccggg cgcgaaactg gtcagctact
     6841 acatcgacct gaagcgggtg atcatgcggc ggctggtcgt cggcgtcgcc gatggcatca
     6901 tgaaagtcga cggcaaggtg atctacgagg cgaaggatct gcgcgtcggt ctgttcaccg
     6961 agcaggcgca atcggcgctc gcgtcggcgg ccgtcccggc ctgagccgat cccggtgggc
     7021 acagacatga agaaggagat cgttgcgtga ggcgcgtggt tgtcaccggc atgggcatcg
     7081 tgtcatcgat cgggaacaac gtgcaggagg tcgcctcctc gctgcgcgag acgcggtccg
     7141 gaatcaccct tgccgaagcc tatcgcgaca tgggctttcg ctgccaggtg catggcagca
     7201 tcaaggtgaa tctggaggag agagttgacc gccgcgcgcg gcgcttcatg ggcgagggcg
     7261 ccgcctacaa ttttctggcg atggaacagg cgttggccga ttccggactt gaagagaagg
     7321 atatcgtcaa tccgcgcacg ggattgatca tgggctcggg cggaccgtcg acgcgggcga
     7381 tcgtcgcggc ggcggatacg gcacgctccg agcctgtgaa aggcccaaaa cgcgttggcc
     7441 cattcgtggt gcccaaggcg atgtcgtcca catgctccgc caatctctcc acctggtaca
     7501 agatcaaggg cgtcaactac tcgatcagct ctgcctgttc gaccagcgcc cattgcatcg
     7561 gcaatgcggg cgaactcatc caatggggca agcaggacat cgttttcgcg gggggcggcg
     7621 aggaactcga ctggacgctc tccgtgctct tcgacgcgat gggcgcgatg tcgtcgcatt
     7681 tcaacgacag gccggaaaag gcctcgcggg ccttcgacag gtcgcgcgac ggcttcgtca
     7741 tttccggcgg cggcggcgtg ctggtgctcg aggaactcga gcatgcgaag gcacgcggcg
     7801 cgaaaatcca tgccgaactc gtcggctacg gtgccacgtc ggacggccac gacatggtgg
     7861 cgccgtcggg ggagggtgcg gtgcgctgca tgcgccaggc gctcgccact gtgaacggcc
     7921 ccatcgatta catcaacgcg cacggcactt cgacgccggt cggcgacgcc acggaaatcg
     7981 ctgccttgcg cgaagtgttc ggatcgcgca tcccgccggt caattcgacc aaatccctca
     8041 ccggccattc gctgggcgcc gcgggcgtgc aggaatccat ctattcgctc ctgcagatgc
     8101 agggcggctt cattgcagag agcgccaata tcgacgagct cgacctggaa atcgccgacg
     8161 cgcccgtcgt gcgctcgcgc atcgacggcg cgaaaatcga gcgcgctctg tcgaacagtt
     8221 tcggttttgg cggcaccaac gccacgctgg ttttcgagcg gtatcacggc tgaatgaaag
     8281 ggaacggaca gagcatgacg aattccaggg gcttgatggg cggacggcgc ggcctggtga
     8341 tgggggtggc caacgaccat tccatcgcct gggggatcgc ccgcgcgctg gcccgccagg
     8401 gcgcggacgt ggccttcacc tatcagggcg aggcgcaggc gcggcggctg aagccgctcg
     8461 cagcctcgat cggctcgcgc attgtgctgc cctgcgatgt cgaagacgac gcgcagctcg
     8521 acgaggcgtt cgccgtgctt cgctcggagt ggggcgcgct cgatttcctc gtgcacgcga
     8581 tcgcgcattc cgacaagaac gagctcaagg gccgctacgt cgacacctcg cgtgccaatt
     8641 tccttcggag cctgagcatt tcctgctact cgttcaccgc ggtcgcccag cgcgcggcgc
     8701 atctgatgac gccgcccgcg gccggagggc atggcggcgc gcttctcacg ctcacctatc
     8761 tgggcgcgac ccgggtgacg ccgaactaca acgtgatggg cgtcgccaag gcggggctcg
     8821 aagcaagcgt gcgctatctc gccgccgacc tcggccgcga cggcgtccgg gtgaacgcga
     8881 tttccgccgg cccgatgcgc acgctggccg gatcggtgat cggcgaggcg cggaccgtgt
     8941 tccgctggaa ccgggcgcac agcccgctca agaagaacat cgaactcgag catgtcggca
     9001 acgccgcgct ctatctcctg agcgacctgt cggcgggagt caccggcgag attcacttcg
     9061 tcgacgccgg ctacaacatc gtgggcatgc cgccgagcgc ggacctgaaa ggctggatcg
     9121 cgccggaaaa tggcgccgag aacgaggatc aggaagaagc cgcaggagat cggcagtctg
     9181 tgcgggtcgg tgtataagct cgctgcactc catagctcac cagggctggt catggtgaaa
     9241 aaaatgagcg tgacgccatc gtgcgattga atcaggtaac cgttcccgcg cacgacctcg
     9301 atgcttcgat tgcgttctac gaaaagctcg gcttccggct gatcgtaaag cacggtccgt
     9361 atgcgcggtt cgaactcccg gacggcgagg cgacgctctc cgtgcacgca acggacaagt
     9421 tcgtgcccgg cgacgtgcac gtctatttcg agtgcgagga tctggacaaa cgcatcgccg
     9481 agctgaaagc gaagggaatc gtcttcgaca gcgatcccga agatcaacgc tggctctggc
     9541 gcgaagcgtg gctccgcgat ccggctggcg tgaagctctg tctatacgtt gccggcgaga
     9601 atcgccgtta tccgccgtgg cggctcaagg gctgagcgtg gccggttatt ccagaacgcc
     9661 gctggcgaag aaactcggca tcagggacgg agcgacgctc tgcatcatcg cggcgccggc
     9721 agagtatcgc gactggctcg cgccgctgcc gcccggcgtg tcgttcctcg ccagggcgtc
     9781 aaattgcgat ctcgtgcatc tgtttgtcac ctcgctgaag gcgctcgagc gcgatctgcc
     9841 gaaggcccgc gcggcgataa aatccgatgg cgcgatctgg gtctcctggc acaagaaatc
     9901 ctcgaagatt ccgacggacg taaccgaaga cgtggtgcgc gcccgcgccc tccggaccga
     9961 cctcgtggat gtgaaggtct gtgcggtgac ggaagattgg tcgggattga agctcgtagt
    10021 gcgcaagcat ctccgttgag cgctcaagca cggtcacgaa agcgttcctc tgccggaatc
    10081 gagggccttc tccctttact tccaacgccg gggatggttc gaaagcactc aggaaccggc
    10141 catcgggctg ggtgcggtct tcggacccgc gtgggccttc cagcctccgc gcaattcgcg
    10201 cctgccgaaa atgatgcaga cggcggcgcc ccgatttgcc tataaggaaa ggtgtccagg
    10261 ggcttggtga tggcgataac cgcgaaaccc gttcgactcc gccaatggcg gaggcttgtg
    10321 tggcccgctc tggcgctgct gctttgcacg ctgcccggct ccgcccagcc ggctggtccg
    10381 atccccccca ggccgggctt tcctggcgga cccccgggcg gcccggcgca gcctggggaa
    10441 ccagccaatt atcctccgaa cgcgcagaac gtgggcatga acgaaacctc gggcggcatc
    10501 acgctcaact tcgtcaacgc cgatgtgcgc gacgtggcga aggccgtgct cggcgactat
    10561 ctgaagctta actacgagat tgccgccaac gtcaccggca cggtcaccat ccagaccagc
    10621 cagccgctcg cccgtgcgca ggtccttccc gcgctcgaac aggccttggg cctcaacggc
    10681 ctcgcgcttg tacactccaa tggcatctac gaagtgttgc cgatcgccga cgcccacaag
    10741 caggtcggca cgacggcgcc gcccgcggtc acgaaaacgc agggctatgg catggaagtc
    10801 gtgcacgtca gatatgtgag cgcgacggcg atgcaaaagc tgctggagcc gattgccggg
    10861 acggaaggcg cgatccggat cgatccggca cgcaacgtgc tcatcatcga aggcactgcc
    10921 cagcagcggc aaacactgct cgaagacatc gcgcttttcg atcaggactg gctcgcgggc
    10981 atgtcgttcg cgctgttcga acccaaatac atggatgccg atgagctggc gaaagagctg
    11041 agcgatctga tcgggggcac cgacagcccg atcgcgggcg tggtgcgctt cgtgccgatc
    11101 gaaaggctga acggagtgct cgccatctcg ccccagcaac gctatctaga ccagctgcgg
    11161 gcgtgggtgg aacgcttcga ccggccgggg gaaggcaatg acaagaagat ctatgtctat
    11221 cgcgtgcaga acgggcgggc gaccgacatc gccgccacgc tgatcaagac attgttcgga
    11281 tccagttcca cggaaccgat acagcagccc gcgcaaaccg gcgcttcgcg cttcaacgct
    11341 acgggcacga ccggcaccac cggcacatcg ggcaccaccg gcacatcggg caccacgggc
    11401 acgaacggca catcgggcac cggcggaccg ggcggcctcg gcgggaatac gggcttcggg
    11461 caaaataccg gcacgggggg gacctcgaat accggcgtgg gcggcaactc gctcactgcc
    11521 aaccgcagcc agagcccggc cgtacccttc gcgccggtca ccacgccggt cgagcagcag
    11581 ggcgcgcgca caggcggccg ctacgagacg gtgggcggcg tgtcgcggga aaacatcagc
    11641 tcggtcaaca tcactgcgga cgaatcgaac aacgcgctgg tgatcctggc cacgccgcgc
    11701 gaatacaagg tgatctccga tgcgctgcgc gagctcgaca tccagccgct gcaggtgctg
    11761 ctcgaagcgg cgatcgccga ggtcaccctc aacaagaaca cgcaatacgg ctttcagtat
    11821 ttctatcagc ccaacagcac gcccaacagc ctgagcacct ttaccttgac caacgccgcg
    11881 acgctcgcgg ctcctcccgg atcgccgctg ctggcggcac tgccgggttt ttcctacatg
    11941 ttcgccaacg gcaacagcct gcaagccgtg ctgagtgcga tcgcgaccga gacgcatgtc
    12001 gaggttctct cggcgccgga agtgctggtg ctcaacaatc agacggcctc gctcgaggtc
    12061 ggcgaccagg tgccgatcgt gacggcaacc tcggtc
//
LOCUS       DASZMC010000451         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2394837,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000451 DASZMC010000000
VERSION     DASZMC010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394837"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VG274_09395"
     CDS             <1..897
                     /locus_tag="VG274_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWE06911.1"
                     /translation="SHATIAWRDLVRGDVEIDTATLSDPVLIREDGRFLYTLPSVADD
                     VDFGVTHIIRGEDHVTNTAAQIEIFEALKAPLPHFAHFPLLVGSGGEALSKRLGSLTV
                     RSLRDEEGIEPLALACYLAKTGTSDAIELRDSVAALAQEFAFEKIGRAPAHFDPAELS
                     HLNAKLLHALPYGAVAMRLSDMNIGGGAAFWEAVKPNLSRMQDAADLWRIVKGPVAPV
                     IEDGQLAEKAAALLPPEPWDEELWTRWTNAIADATGAKGRALFHPLRLALTGCEHGPE
                     LKKLLPLIGRAKALARLNGETA"
     gene            984..>1690
                     /locus_tag="VG274_09400"
     CDS             984..>1690
                     /locus_tag="VG274_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWE06912.1"
                     /translation="MILRLYNTLTRAKEEFAPLDPPHVRMYVCGPTVYDFAHIGNARP
                     VIVFDVLFRILRHLYGEAHVEYVRNVTDIDDKINARAQEKRGGRAILDAVRELTSETG
                     RLYHEDVAALGCLPPTHEPRATEFVPGMISLIKRLVAGGHAYEAQGHVLFDVSSKPDY
                     GKLAHRSVDEMIAGARVEIAPYKRGPMDFVLWKPSDESLPGWPSPWGRGRPGWHIECS
                     AMSEALLGETFDIHGGG"
BASE COUNT          317 a          522 c          530 g          321 t
ORIGIN      
        1 agccacgcga caatcgcctg gcgcgatctc gtgcgcggcg atgtcgagat cgacacggca
       61 acgctctccg atccggtgct gattcgcgaa gacggacgat tcctctacac cttgccctcg
      121 gtggccgacg atgtcgattt cggcgtcacg cacatcattc gcggcgaaga tcacgtgacc
      181 aacaccgcgg cacagatcga gatcttcgag gcgctcaaag cgccgctccc gcatttcgcg
      241 cattttcccc tgctcgtcgg atcgggcggc gaggcgcttt ccaaacgcct cggctctctg
      301 accgtcagat cgctgcgcga cgaggagggc atcgaaccgc tcgcgcttgc ctgctacctc
      361 gccaagaccg gaacgtcgga tgccatcgaa ctcagggatt cggtcgccgc gctcgcgcag
      421 gagtttgcct tcgagaaaat cgggcgcgcg ccagcgcatt tcgatccggc cgagctctcc
      481 catctgaacg ccaagcttct gcacgctctg ccatacgggg ctgtcgccat gcggctctcg
      541 gacatgaaca tcggcggggg cgccgcattc tgggaggcgg tgaaaccgaa cctgtcgcgc
      601 atgcaggatg cggcagacct gtggcgcatc gtcaagggtc cggttgcgcc cgtcatcgag
      661 gatgggcagc ttgcagagaa ggcggcggca ctgctgccgc ccgaaccctg ggacgaagaa
      721 ttgtggactc gatggacgaa tgcgatcgcc gatgcgacgg gagcgaaagg ccgtgcgctg
      781 tttcatccgt tgcggctcgc gctcacaggc tgcgagcatg gaccggaact gaaaaagctg
      841 ctaccgctga tcggccgcgc gaaagcgctg gcacggctca atggggaaac cgcgtgatgc
      901 tcgcgctctg gtgttgcatt tcagtccgac gctgacgggt ctttgtttcc gatcgacaac
      961 cagatttgca aagcagcttc cccatgattc ttcgtctcta caacacgctg acgcgagcga
     1021 aggaagagtt tgccccgctc gatccgcccc acgtgcggat gtatgtgtgc ggcccgacgg
     1081 tttacgattt cgcccatatc ggcaatgcgc ggccggtgat cgtattcgat gtgctgttcc
     1141 ggatactgcg ccacctttat ggagaagcgc atgtcgagta tgtgcgcaac gtcaccgaca
     1201 tcgacgacaa gatcaacgct cgtgcccagg agaaaagagg cggccgcgcg atcctcgatg
     1261 cggtgcgcga gctgacatcg gaaaccggcc gcctatatca tgaagatgtg gcggctctgg
     1321 gatgccttcc gcccacgcac gagccgcggg cgaccgaatt tgtgcccgga atgattagcc
     1381 ttatcaaacg ccttgtcgct ggcgggcatg cttacgaagc ccagggccac gtgttgttcg
     1441 atgtctcgtc gaagccggac tacggcaaac tcgcccatcg ttccgtcgac gaaatgatcg
     1501 ccggcgcgcg ggtcgaaatt gcaccctaca agcgcgggcc catggatttc gttctgtgga
     1561 agccttcgga tgaatcgctt ccgggctggc cgagcccctg ggggcgtgga cggccaggct
     1621 ggcacatcga atgctcggcg atgagcgaag cgctcttggg cgagacgttc gatattcacg
     1681 gcggcggcat
//
LOCUS       DASZMC010000452         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_996614,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000452 DASZMC010000000
VERSION     DASZMC010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996614"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VG274_09405"
     CDS             <1..921
                     /locus_tag="VG274_09405"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWE06913.1"
                     /translation="VVGGNLLTNGPLAYLLRNRVYLGEINHRDQSYPGEHEPILDPDL
                     FESVQAKLSENRRGLRQRRQSSNALLLGKLFDDRGNSMTPSYAIKKGVRYRYYVSCVL
                     AQGRKEEAGSVARVAADAVERIVLDAVRDRGPDPDTAAATIAARIGKATLGALSIELQ
                     LIDDGDRPPEKIAIPWSPEPFRRKRAVIAPANDGAHGSRPIRAEARTKLLAAIARARR
                     WLEDLISGRAASIETIAVREGVSERSARIALSFAFLAPAIVQATLDATLPRGFDVSRL
                     TDMPASWAEQRKLLGLAAPRFCEQRNPSSV"
     gene            1878..2435
                     /locus_tag="VG274_09410"
     CDS             1878..2435
                     /locus_tag="VG274_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HWE06914.1"
                     /translation="MPIEGYPRKGVVAAPNGLTPALKAKSRTRRERQRALAEIADHPP
                     KRRNDLLPQLAVSYVPIDELRQTQRRVRSSDPAQVARIRASIQKFGVCQPILIGKDRT
                     IVHGHGVVEAARAAGLKQIPVIAVEHLSPAELRLLSITLSRLGETGRWDEEALRTEFA
                     ELIDLGEDVVVSGFGDGGNRVPAAR"
     gene            2578..>2840
                     /locus_tag="VG274_09415"
     CDS             2578..>2840
                     /locus_tag="VG274_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06915.1"
                     /translation="MLPGEQARLVLTDEPYNVPNVGHVTSQAHHREFAMAAGEMSREQ
                     FVAFNRAWMSSAALYVEDGGLIGTFIDWRSVELVLACGRDLGF"
BASE COUNT          522 a          913 c          898 g          507 t
ORIGIN      
        1 gtcgttggcg gtaacctcct caccaatggg ccgttggctt acctgctgcg caaccgggtc
       61 taccttggcg agatcaacca tcgcgaccaa agctatcccg gcgagcacga accgatcctg
      121 gacccggacc tgtttgagtc cgtgcaggcg aagctctctg agaaccggcg gggccttcgg
      181 cagcggcgtc aaagctcgaa tgccttgctt ctgggtaagc tcttcgacga tcggggaaat
      241 tcgatgacgc cgagctacgc gatcaagaag ggggttcgtt atcgctacta tgtctcctgc
      301 gtcctggccc agggccgcaa ggaagaagcc ggatcggtcg cgcgcgtcgc cgcggacgca
      361 gtcgaacgga tcgttctcga tgcggtccgc gatcgcggcc cggatccgga tacagccgcc
      421 gcaacgattg ccgctcggat cggcaaagcc actctaggcg cactgtcgat cgagcttcag
      481 cttattgacg acggcgatcg gccgcccgaa aagatcgcaa tcccctggtc tccggaacca
      541 tttcgccgca agcgcgcggt gatcgcccct gcgaacgatg gcgcgcatgg ctcaaggccg
      601 atccgggccg aagcccggac gaagctgctg gcggccatcg ccagagcgcg ccggtggctc
      661 gaggacctca tctcaggccg cgccgcgagc atcgaaacga ttgcggttcg cgaaggggtc
      721 agcgagcgat cggcgcgaat tgctctctcc ttcgccttcc tggccccagc catcgtccaa
      781 gctaccctcg atgcaacctt gccgcgcggc ttcgacgttt cgcgattgac ggacatgccg
      841 gcgagctggg ccgagcaacg aaagctcttg ggcttggctg ccccgcgctt ctgcgagcag
      901 cgaaacccat caagcgtttg acaagcttcg tcctcgcgtc atgtcccaat gcgagcgcgt
      961 tcatgtgtct ggcgcttacg aattccgtga ccgccgacac cgctcggtaa ctcgtctgtc
     1021 tacgcgatct ctcatgaagg gccagaatcg aaccgctctc ctcgaccgag cgaaccggtc
     1081 tccgtctgac cccatgcaac atcccacgga aaaggttttt gtcaggcgga gacacggcgt
     1141 cgccggagcg gtacgagccg caaattcggc gtctcccgac gcgaatccac gtgcgctcag
     1201 cgcccaagcc tcggaaatgt cggacagatt gccggagaca gaaaaacgac gcaaatcacg
     1261 gagatgggtg gtagtggacg cagtcccgcg gaaccggtct ccgtcaggaa aattccctgg
     1321 ggtcagggaa ttttgcaggg agtttacccg aagaccgctt tttgcgcggt cgggtggtcg
     1381 agttaagcat cggaaattgc gtactaattt gcgcgattgc ccgcaaattc cctactggcc
     1441 ggggcaggga atagcaggga attgaggtcc ggcggcgcca aggcttggct tcggcctttt
     1501 gccgctgtcc acctttgcgg tggctggcgc gcgatcccgg cggtcccggc tctggttgct
     1561 ggcactcaac acgaacaacc aagcaggacc ggcatgcacg cgttttttcc ggcgttaact
     1621 attcccgagc cttgcaggtt catctcacgt gcctcatata tcccctcgga tctccgctgc
     1681 ctgagggcga gcggtcatcc aagcgagacg acgcttccag actaggatgg tcggaggtcc
     1741 gggcgctcga agatgagtga tcgcggccca gggggtcggg ttgactgctt cgaggcccag
     1801 attccgtccc tcggttgggt ggtcgcctct tcctcttcag acaacctgtt ccggaaaccg
     1861 agaagaggaa gtgaccaatg ccgatcgaag gatacccccg caaaggcgtc gtcgcggccc
     1921 ccaacggcct cacgccggct ctgaaagcaa agtcgcgcac ccgccgggaa cgccagcggg
     1981 cgctcgccga gatcgccgac caccctccga agcggcgcaa tgacctgctg ccacagctgg
     2041 cggtgtccta tgtgccgatc gacgagctgc gccagacgca gcgtcgcgtg cgcagttcgg
     2101 accccgcgca agtcgctcgg atccgcgcca gcatccaaaa gtttggcgtc tgccagccga
     2161 tcctgatcgg caaggacagg acaatcgtcc acggccacgg ggtcgtcgag gcggcgcgcg
     2221 ccgccggcct gaagcagatc ccggtcatcg cagtcgagca tttgtcgccg gcggagctgc
     2281 gcttgctgtc aatcacgctc agccgtctcg gcgaaaccgg gcgctgggac gaagaggcgc
     2341 tgaggaccga attcgcggag ctgatcgatc tcggcgagga cgtcgtggtt tcggggttcg
     2401 gagacggcgg aaatcgagtt cctgctgctc gatgacgagt cggacgacgg gacgggggaa
     2461 ctcgatccgg ccgcagcggc ggaagcagcc gtctcgcggc caggtgacct ctggctcctc
     2521 ggccgccatc gccttctgca ggccgacgcc cgcgattcgg cgagctacga acggctcatg
     2581 ctgccgggcg aacaggctcg tctcgtgctg accgacgaac cctacaatgt tcccaacgtc
     2641 ggacatgtga cctcccaggc ccatcaccgg gaattcgcga tggcggccgg cgaaatgagc
     2701 cgcgagcaat tcgtggcgtt caaccgcgcc tggatgtcca gcgcggcgct ctacgttgaa
     2761 gacggcggcc tgatcgggac gttcattgac tggcgcagcg tcgaactggt cctcgcctgc
     2821 ggtcgcgacc tcgggttcga
//
LOCUS       DASZMC010000453         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4591555,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000453 DASZMC010000000
VERSION     DASZMC010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4591555"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VG274_09420"
     CDS             <1..538
                     /locus_tag="VG274_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE06916.1"
                     /translation="TELLDFATTAKATAYLAVNPMGKVPAIKHGDVVVTESAAICAYL
                     ADAFPAARLAPPSGDRLRGPYYRWLFFGAGPVESAATNKAFGFVVPDDRKGMAGYGSL
                     AQVMDVLESAVSRGDYLVGGNFTAADLYLGSHIGWGMQFGTLEKRPAFERYWDRISKR
                     PAAVRAREIDDALIPRKE"
     gene            699..1244
                     /locus_tag="VG274_09425"
     CDS             699..1244
                     /locus_tag="VG274_09425"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HWE06917.1"
                     /translation="MSTQMLKPRLMLVAAMILAAAASRLLPHPPNLTSVTAVALFGGA
                     CLSDRRPAFLVPLSALFLSDLVLGFHDQMALVYGCFALTVCIGLWLQQRRSAPAIAGA
                     ALASSVLFFIVTNFGVWALDNIYPRTFAGLIACYTAALPFFRNTLEGDLLYTLALFGG
                     FALLERQFPALRSETAEPLAA"
     gene            complement(1251..>2991)
                     /locus_tag="VG274_09430"
     CDS             complement(1251..>2991)
                     /locus_tag="VG274_09430"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWE06918.1"
                     /translation="IAYGADFHDMFEVRGAQRPARGQILAPRLSDRSVVLGYEGLDRM
                     ARSTAIAFSAMPERIGGDRAEFRITLAPGARYELYIEIGPEEADVPARNRYRSAAARA
                     RSSMRRSRRQGGGMRSSGRLFDDWMERSRADLALLTTQLATGPFPYAGIPWFSTPFGR
                     DAIITSLQILWLDPGLARGVLAFLAQTQAHERSAFQDSAPGKIMHEARKGEMTTLKEV
                     PFGQYYGAVDTTPLFVMLAGAYAERTGDLETIEQLWPSIEAAIFWIENEQAMDPDGFV
                     TYRSAGAGLVNQGWKDSNDSIFHADGTLAQGPIAVVEVQGYAFAAFRAMAALCDRRRD
                     SETAARWRKRAENLRAAVEAQFWMDDLGFYAIARDGERQTCRVRASNPGHLLYTGLPS
                     RKRARRVIEQLLSPAFNSGWGIRTLAQGEARYNPMSYHNGSVWPHDMALCAAGFARYG
                     DRQGVVRLMSEMFEAAVRFDMRLPELFCGFPRSPGGPPIAYPVACLPQAWSSGSAFMI
                     LQSCLGLRVDGLRGEIHVAQPMLPVGIDSIVLRHLFVGNASADIIFHRVGERVVAFPY
                     SKDSGAVPLFIHA"
BASE COUNT          542 a          992 c          921 g          536 t
ORIGIN      
        1 gacggagctg ctcgatttcg cgaccaccgc gaaagcgacg gcttatcttg ccgtcaatcc
       61 gatgggcaaa gtccctgcca tcaagcatgg cgacgttgtc gtcaccgaat ctgcggccat
      121 ctgcgcctat ctcgccgacg cgttccctgc tgcccgtctc gcgccgcctt cgggcgatcg
      181 tctgcgcggt ccctattacc gctggctgtt cttcggcgcc ggtccggtcg aatccgcggc
      241 gacgaacaag gctttcggct tcgtcgtacc cgacgaccgc aaaggaatgg cgggttatgg
      301 ttcgctcgcc caggtcatgg acgttctcga aagcgcggta tcccgcggcg actacctggt
      361 tggcgggaat ttcactgcag ccgatcttta cctcggttcc catatcggct gggggatgca
      421 attcggaacg ctcgagaaac ggccggcctt cgagcgctat tgggaccgca tcagcaaacg
      481 tccggcagcg gtgcgagcgc gcgagatcga cgatgcgctg atacccagga aggaatgaaa
      541 cagcgcccgc acggccgatc gatcctgttg atcgagcctg tcgatcgatc cttgcggtgg
      601 catgggcgaa ttcgtacctt ggcatcagct ctcatggttc gacaggctcg ccatgagggc
      661 ctcgccatga gcctgccgaa ggaagaggct cgagccacgt gtccacgcaa atgctgaaac
      721 cccgattgat gctggtggcc gcgatgattc tggctgccgc tgcatcgcgc ctgctcccgc
      781 atcccccaaa cctgacctcg gtgacggcag tcgccctctt cggcggcgca tgcctttccg
      841 atcggcggcc ggccttcctc gtgccgctga gcgcactttt tctcagcgac ctcgtgctcg
      901 gattccacga ccagatggcg ctggtctatg gctgctttgc gctgacggtc tgcattggtc
      961 tgtggctgca acagcgccgc tctgcgccgg cgatagccgg cgcggcgctt gcaagctcgg
     1021 tgctgttttt catcgtcacc aatttcggcg tctgggcgct tgacaacatc tatcccagga
     1081 cgttcgcagg actgatcgcc tgctacactg cggccctccc cttcttccgc aatacgctcg
     1141 aaggcgatct cctgtacacg ctcgccctgt tcggcggctt cgcgctgctg gagcgccagt
     1201 tccccgcatt gcggagcgag accgccgaac ctctggcggc gtgagagcat tcaggcgtgg
     1261 atgaacagcg gaacggcgcc tgaatctttg ctgtacggaa aagcgaccac gcgctcgccg
     1321 acgcgatgaa agataatgtc cgcgctcgcg tttccaacga agagatggcg cagaacgatg
     1381 ctgtcgattc cgaccggcag catcggctga gccacatgga tctctccgcg cagaccatcg
     1441 acgcgcaagc caaggcacga ctgaaggatc atgaaagccg accccgacga ccacgcctgc
     1501 ggcaaacagg cgacgggata ggcgatcggc gggcctccag gcgaacgcgg aaagccgcag
     1561 aacagttccg gcagccgcat gtcgaaccgc acagcggcct cgaacatctc actcatcagc
     1621 cgcaccacgc cctgcctgtc gccgtagcgc gcgaatccgg ccgcgcacag tgccatgtcg
     1681 tgcggccaga ccgatccatt gtgatacgac atgggattgt aacgcgcctc gccctgcgcc
     1741 agcgtccgga tgccccagcc cgaattgaat gcgggcgaaa gaagctgttc gatcacgcgg
     1801 cgcgcgcgct tgcgcgatgg cagccccgta tagagaagat gtcccggatt tgacgcccgc
     1861 acgcggcaag tctggcgctc cccgtcgcgc gcgatcgcat agaagccaag atcgtccatc
     1921 cagaactgcg cctcgacggc ggcgcgcaaa ttctcggcgc gttttcgcca gcgcgcggca
     1981 gtttcggaat cgcgcctccg gtcgcagagc gccgccatcg cccgaaaagc ggcgaaggcg
     2041 tagccttgga cctccacgac agcgatgggg ccctgcgcaa gggtgccatc ggcgtggaag
     2101 atcgaatcgt tgctgtcttt ccagccctgg ttgaccagac cggcgccggc ggaacgatag
     2161 gtgacaaatc cgtcgggatc cattgcctgt tcgttttcga tccagaaaat cgccgcctcg
     2221 atcgaaggcc acaattgctc gatcgtttcg agatcgccgg tacgttccgc ataggcgccc
     2281 gccagcatga cgaagagcgg cgtggtgtcg acggcgccgt aatactgacc gaagggaacc
     2341 tctttcagag tcgtcatctc gcccttgcgg gcttcgtgca tgatcttgcc gggcgccgaa
     2401 tcctggaatg ccgagcgttc atgcgcctgg gtctgcgcga gaaaggcgag cacgccgcgc
     2461 gcgagacctg ggtccagcca caggatttgc agcgaggtaa tgatcgcatc gcgcccgaac
     2521 ggcgtcgaaa accacggaat gccggcatag ggaaagggcc cggtcgccag ctgcgtcgtc
     2581 agcagcgcca gatcggcacg cgagcgctcc atccagtcgt cgaacagccg gcccgacgaa
     2641 cgcatgccgc cgccctgacg gcgcgatctg cgcatgctcg atcgcgcccg tgccgcagca
     2701 gagcgatagc gatttcgggc gggaacgtct gcctcctccg gtccgatctc gatgtacagc
     2761 tcgtagcgcg cacccggcgc cagcgtgatc ctgaattcgg cgcggtctcc cccaatgcgc
     2821 tcgggcatcg ccgagaaggc gatagccgtc gagcgcgcca tgcggtcgag gccttcgtag
     2881 cccaggacca cgctgcggtc ggacagcctg ggcgccagaa tctggccgcg cgccggccgc
     2941 tgcgccccgc gaacttcaaa catgtcgtga aaatctgctc cataggcgat g
//
LOCUS       DASZMC010000454         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2662885,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000454 DASZMC010000000
VERSION     DASZMC010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662885"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VG274_09435"
     CDS             <1..792
                     /locus_tag="VG274_09435"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWE06919.1"
                     /translation="SQYTEEFAYYRTYFSGSVTGLGKGTLVRYNGIQVGNVSALEFDP
                     NDPKRVIVTLQVDPKLQIHSDSLASIASEGLTGGTYVEIDGGSQSAPILPRTMFGEYP
                     VIRSKRSTLQELEQSAPQVLAKIGRIADRLNDVLSDKNRQAFAQTLSNLRDVTATLAR
                     HSSDLDRIMANVATASTGLNTDIGDLHNVLTHAGQTEAKIDRLADSFDRLSGDLDTQV
                     NGARIDQLMGEARELVRSLTHLSDELEHEPTRLLYGDRRKGYTPP"
     gene            789..1418
                     /locus_tag="VG274_09440"
     CDS             789..1418
                     /locus_tag="VG274_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HWE06920.1"
                     /translation="MSDPTFVPTRRHMVLAGAAMLALAGCGSLLGPSSPPLQLYRLRP
                     AFPAAGGPFVSWQLAVARPSAPQSLDTERIALMRGALMDYYADAQWSDSVPRLVQSLL
                     VEAFERSGRILAVAAESGGVHGDYTLECEIRDFDAQYATDNGAPTVIVAIEAKLLGPQ
                     GKVVASLEAKQAEPASANSVPSVVAAFDQAIAATLTQIVTWALGAPPPK"
     gene            1584..2000
                     /gene="mscL"
                     /locus_tag="VG274_09445"
     CDS             1584..2000
                     /gene="mscL"
                     /locus_tag="VG274_09445"
                     /inference="COORDINATES: protein motif:HMM:NF010557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWE06921.1"
                     /translation="MFDEFKKFAMRGNVIDLAVGVVIGAAFGAIVTSLVKDVIMPPIG
                     WAMGGIDFANFFLTLKGPAAATLAEAQKAGAVTVNYGVFVNAVVNFLIVALALFLLIR
                     AINQLQTKPPESPAAAPPPPEDVVLLREIRDLLKAR"
     gene            2267..2512
                     /locus_tag="VG274_09450"
     CDS             2267..2512
                     /locus_tag="VG274_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWE06922.1"
                     /translation="MDESVSASDANRKFSLLLRRVREGQSYVVTSHGKPVARLIPPGK
                     HDKAAADARSALLARLRKQRVVHAGRWTRDELYEADR"
     gene            2509..2958
                     /locus_tag="VG274_09455"
     CDS             2509..2958
                     /locus_tag="VG274_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWE06923.1"
                     /translation="MKAAVDTNILAYAEGVNGTKRRDSALDLLRRLPHEAAVIPVQAL
                     GELFNVLVRKAGKSRKHARNALLSWSDACPVVQTSPEGMAAAAGLATDHRLGIWDAVI
                     LSAASQAGCRLLLSEDLQEGFTWGGVTVVNPFAPSPHALLKALLGNE"
     gene            3548..>3870
                     /locus_tag="VG274_09460"
     CDS             3548..>3870
                     /locus_tag="VG274_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HWE06924.1"
                     /translation="MAQTETVLHSFRGGGHPGGRLLLGKSGALFGTASGGTLSLGNAY
                     ELVQRQGVWRRIRLHRFQGTDGSKPAAGLVQGSDGSLYGTTESGGTDGFGTVFELTRS
                     QGIWT"
BASE COUNT          691 a         1254 c         1225 g          700 t
ORIGIN      
        1 tctcagtaca cggaggagtt cgcctattac cgcacctatt tctccggcag cgtcaccgga
       61 ttgggcaagg gtacgctggt gcgttacaac ggcatccagg tcggcaacgt ctccgcgctc
      121 gagttcgatc cgaacgatcc caagcgggtg atcgtcaccc ttcaggtcga tccaaagctc
      181 caaatccatt ccgattcgct ggcctcgatc gccagcgaag ggctgaccgg cggcacctat
      241 gtggagatcg atggcggttc gcaatcggcg ccgatcctgc cgcgcaccat gttcggcgaa
      301 tatcccgtga tccgctcgaa acgctcgacg ctgcaggagc tcgaacagtc cgcgccgcag
      361 gttctcgcca agatcggccg catcgccgac cggctgaacg acgtgctgag cgacaagaac
      421 cgccaggcct tcgcgcagac gctgtccaat ctgcgcgacg tgaccgcgac gctcgcgcgg
      481 cattcctccg atctcgacag gatcatggcc aatgtggcaa ccgcctccac cggcctcaac
      541 accgatatcg gcgatctgca caatgtgctc acccatgccg ggcagacgga agcgaagatc
      601 gaccggcttg ccgattcctt cgaccggctg tccggcgatc tcgatacgca ggtgaacggc
      661 gcacgcatcg accagctgat gggcgaggcg cgcgaactgg tgaggagcct cacccatctc
      721 tccgacgaac tcgaacacga gcccacgcgc cttctctacg gcgatcgccg caaaggatat
      781 acgccgccat gagtgatccg acgttcgtgc cgacgcggcg ccacatggtg cttgccggcg
      841 ccgcgatgct ggcgctcgcc ggctgtggca gcctgctcgg cccatcgagt ccgcccctgc
      901 agctctatcg gctgagaccc gcgttcccgg cggcgggagg gcctttcgtt tcctggcagc
      961 tcgcggtcgc acggccgtcg gcgccgcaaa gcctcgacac cgaacgcatc gcgctgatgc
     1021 gcggcgcgct gatggactat tatgccgacg cgcaatggag cgattccgtg ccgcgtctcg
     1081 tgcagtctct gcttgtcgag gcgttcgaga ggagcgggcg cattctcgcc gtggcggccg
     1141 aatccggcgg cgtgcacggc gattacacgc tcgaatgcga gatccgcgat ttcgacgcgc
     1201 aatatgccac cgacaacggc gcgcccaccg tcatcgtcgc aatcgaggcg aagcttctcg
     1261 ggccgcaggg caaggtggtg gcgtcgctcg aggcgaagca agccgagccc gcttcggcga
     1321 acagcgtgcc gagtgtcgtt gcggccttcg accaggcaat agccgccacg ctcacccaga
     1381 tcgtcacctg ggctctcggc gccccgccac ccaaatagta tcgagcgtgg aacacgggaa
     1441 agaaagattg aacgcggagg tcgcggtggt tgcgcggagg acgcaggggg aaatcgcgcg
     1501 cctgtggcgc gcaatccgtt tttcggcgcc ctctgcgcat cctctgcgtc ctctgcgtta
     1561 agtctttttt cagagaggga tcgatgtttg atgagttcaa gaagttcgcg atgcggggca
     1621 acgtcatcga cctcgcggtc ggcgtggtca tcggcgcggc gttcggcgcc atcgtcacct
     1681 cgctcgtcaa ggacgtcatc atgccgccca tcggctgggc gatgggggga atcgattttg
     1741 cgaatttctt cctgacgctg aagggtcccg cggcggcgac gcttgccgaa gcgcagaagg
     1801 cgggcgcggt gaccgtcaat tacggcgtgt tcgtcaacgc cgtcgtcaat ttcctgatcg
     1861 tggcgctggc gctgttcctg ctgatccgcg ccatcaacca gctgcagacc aagccgcccg
     1921 aatcgcccgc cgccgcgccg cccccgcccg aagacgtcgt tcttctccgc gaaatccgcg
     1981 atctgttgaa ggcgaggtag tccgcaacgc cccctccgcc ccttcggggc accccgcact
     2041 tgatgcgggg tgcccccgaa gcgggcggag gggcaggcgc cgcgccgtgt atgatcgccg
     2101 cgcgattccc agcggcgcgc gcttcagcca atgatcgagc ttcgcgagct ttccaaatcc
     2161 tacgccgcgc gcgggccgga ggggaatcgc cgctgcggct tcggcccttg cccgctgcgc
     2221 tggagcgctc tattgtaggc aatataacta cattatagga attgacatgg acgagtctgt
     2281 ctcggcgtcg gatgccaacc gcaaattctc ccttctgctg cgcagggttc gcgagggaca
     2341 aagctatgtc gtgacaagcc acggcaagcc cgtcgctcgc ctcatccctc ccggcaagca
     2401 tgacaaggcc gcagccgacg ctcgctccgc gctgcttgcc cgcctccgga agcagcgcgt
     2461 cgtgcatgcc ggacgctgga cgcgcgacga gctttacgag gcggatcggt gaaggccgcg
     2521 gtcgacacga atattcttgc ctacgcggaa ggggtcaacg gaacgaaacg gcgggacagt
     2581 gctctcgatc tccttcgcag actgccgcac gaggcggcag tcatcccggt acaggcgctc
     2641 ggcgagctgt tcaatgtgct ggtgcgcaag gcgggaaaat cgcggaagca cgcgcgcaat
     2701 gcgctgctca gctggagcga cgcctgtccc gttgtccaga cgtcgcccga aggcatggcg
     2761 gctgcagccg gcctcgcaac ggatcatcgt ctcggcattt gggatgcggt catcctgtcg
     2821 gcggcttcgc aggccggctg ccggctgctg ctctccgaag atcttcaaga gggcttcaca
     2881 tggggcggcg taaccgtggt caatccgttc gcgccttcac cgcacgcatt gctgaaggcg
     2941 ctgttgggaa acgaatgagg cgaggcagac ggtggtgcct gcaggcggta gtgcctgcgt
     3001 tgccccctcc gcctcgcccg gcggaatgcc gggctcgccc cctccgcccc ttcggggcac
     3061 ctcccccacg ccaaagcgtg ggagaggaaa atcgcttttc tttcctcccc agcgcgcgaa
     3121 gcgcggggga ggtgcccgcg cagcgggcgg agggggccga cgtcgcgccg tgtatgatgc
     3181 cggagtgatc ccgcgaacct tccacagcct acgcgccgcc cacgccgccg tccgcgaggt
     3241 cgacaatggc gcctgcttgg ctgaggcggc acggaggatg aaagaacgat gaaacactac
     3301 aggtgcgccg aacctctcat caggagccgc atctccaagt tccgttgggc gcgacgatga
     3361 attccctggc ctcagaattt caagcacagt gtttgttggc gcgcgcacga atttcgactg
     3421 ccacatattg tcagttacat cgttcatcgc tatcgttcgg ttgtgaattc taggtagtgg
     3481 aatctaaatg cgcagtcttc aaataatctc cttggcgatt gcgtgcgcaa tcgcgcagac
     3541 aagcgctatg gcacagaccg aaaccgtcct gcacagcttc cgcggcggcg ggcatccagg
     3601 gggtcgcctt ttgctgggca agagcggagc gctgtttggc actgcttccg gcgggaccct
     3661 ctcacttggc aatgcctacg aacttgtgca acggcaagga gtgtggagga ggatccggct
     3721 tcaccgtttc cagggcaccg acggatcgaa gcccgccgcg ggtctggtgc aaggttcaga
     3781 tggatcgctg tacggtacaa ctgaatcggg cggtaccgat ggcttcggaa cagtattcga
     3841 actgacaagg tcccaaggaa tttggacaga
//
LOCUS       DASZMC010000455         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3216077,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000455 DASZMC010000000
VERSION     DASZMC010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3216077"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VG274_09465"
     CDS             <1..550
                     /locus_tag="VG274_09465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWE06925.1"
                     /translation="ILGGKSRSFGNLHGHLKGMTSVKSLSHWVSGQLDAGLSWKDLEW
                     IRKLWRGPLIIKGILDVEDARCAVGAGADAIVVSNHGGRQLDGAPSSIAMLPKIADAV
                     GSETEVLVDSGVRSGHDVLRAVALGAKACLIGRAYIYGLGAGGEAGVAKAIDIIRREL
                     DATMALTGCKSIADIGPHALVR"
     gene            729..1775
                     /locus_tag="VG274_09470"
     CDS             729..1775
                     /locus_tag="VG274_09470"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HWE06926.1"
                     /translation="MSTVLVTGGSGFIGSHCILQLLAAGHTVRTTLRNLKREDEARSM
                     LREGGAEPGERVSFVAADLESDAGWPQAVAGCDCVLHVASPFPQGVPKDANELIVPAR
                     EGALRVLRAARDAGVKRVVLTSSFAAIGYGQKPRSAPFDETNWTDPDAAGVQPYAKSK
                     TLAERAAWDFIAREGGSLELSVVNPVGVFGPVLGPDYSTSILLVQRLMDGAMPGVPRL
                     TFGVVDVRDVADLHTRAMTHPAAKGERFLAVSGDFMSIREIAMVLKERLGLAARRVPT
                     IQLPDFVVRVAALGDPAVKQFLPELGKVKNATGEKAKRLLGWAPRPREEAIIATAQSL
                     LRLGLLKESAKKAA"
     gene            complement(1870..2580)
                     /locus_tag="VG274_09475"
                     /pseudo
     CDS             complement(1870..2580)
                     /locus_tag="VG274_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
BASE COUNT          476 a          799 c          871 g          467 t
ORIGIN      
        1 catcctcggc ggcaagagcc ggtcgttcgg aaatctccat ggacatctca agggaatgac
       61 cagcgtgaaa tcgctgtcgc actgggtcag tggacagctc gacgcggggc tcagctggaa
      121 agacctggaa tggatccgca agctgtggcg ggggccgctg atcatcaagg gcattctcga
      181 cgtggaagac gccagatgcg ccgtcggcgc gggcgccgat gcgatcgtgg tctccaatca
      241 tggcgggcgt caactcgatg gcgcgccatc ttcgatcgcg atgctgccga aaatcgccga
      301 cgcggtgggc tcggagaccg aagtgctggt cgacagcggc gttcgctccg gccacgacgt
      361 gctgcgcgct gtggcgctcg gcgcgaaggc ctgtctgatc ggccgcgcct atatttacgg
      421 gcttggcgct ggcggcgaag cgggcgtggc gaaggccatc gacatcatcc gccgcgagct
      481 cgacgccacc atggcgctca ccggctgcaa atccatcgcc gatatcggcc cgcacgcgct
      541 ggtgcggtaa caagacaaag taggttcaca gggagacagt gagacggtga ggaagcatgg
      601 cgcgccaaag acgcgcaata attctcagtt ctcactgtct cactgcctcc ctgtgaattt
      661 tctaccttgt tggcggctca gcggtttatc gtcgaactct aggcgcaaat aaaataggga
      721 ggcggcacat gagcacggtt ctggttacgg gcgggtcggg attcatcggc agccattgca
      781 tcctgcagct gctggccgcg gggcataccg tgcgcacgac gttgcgcaac ctgaagcgcg
      841 aggacgaggc gcgcagcatg ctgcgggagg gcggcgcgga gcccggcgag cgcgtgtcgt
      901 ttgtcgccgc cgatctcgaa agcgatgccg gctggccgca agccgtcgcc ggctgcgact
      961 gtgtgctgca cgtcgcctcg cctttcccgc agggcgttcc caaagacgca aacgaattga
     1021 tagttccggc gcgcgaaggc gcgctccgtg tgctgcgcgc cgcgcgcgac gccggcgtga
     1081 agcgtgtggt gctcacctct tcgttcgcgg cgatcggcta tggccagaag ccgcgcagcg
     1141 cgcccttcga cgagacaaac tggacggacc cggacgccgc cggcgtgcag ccctatgcga
     1201 aatcgaaaac ccttgccgaa cgcgccgcct gggatttcat cgcgcgcgaa ggcgggagcc
     1261 tcgagctctc ggtcgtcaat ccggtcggcg tgtttggccc cgtgctggga cccgattatt
     1321 cgacgtcgat cctgctcgtg cagcgcctga tggacggcgc catgcccggc gttccgcggc
     1381 ttactttcgg cgtggtcgat gtgcgcgacg tggcggatct gcatacccgc gccatgacgc
     1441 atcccgccgc aaagggcgag cgcttcctcg ccgtctcggg cgatttcatg tcgatccgcg
     1501 agatcgcgat ggtgttgaaa gaacggctgg gcttggcagc caggcgggtg ccgacgatac
     1561 agcttcccga ctttgtcgtg cgcgtcgcgg cgttgggcga tccggcggtg aagcagtttc
     1621 tgcccgaact tggcaaggtg aagaacgcca ccggcgaaaa ggcgaaacgc ctgctcggct
     1681 gggcgccgcg cccgcgcgag gaagccatca tcgccaccgc ccaaagcctg ctgcgcctcg
     1741 gactgctgaa ggagagcgca aagaaggcgg cgtagaattt tgttcaccta agcactgagc
     1801 tctgatcgaa gcgggagctg acctccccac aagggggagg ttggcgcgtt cgtcgagcgc
     1861 gtgactcaac tattccgaat cgcgccactc caccgacgcc gcggggcggc ggttgatggt
     1921 gagctggaag acgtcaaggc gggcatagtg gccggtggtg tcgagcgtac ggcgcgcggc
     1981 ggcgatgcgt gcgagatcga tgtccgcgta aaggatgccg tgctccttgt gcagcggtcc
     2041 ggcgacaatc attccgcccg gcgcgacgac gacggaatcg cccggatgta tccactcctc
     2101 cgtgtcggga tagagccgcg gcttgccggg aaacgtatcc ggaatatccg agcctttcag
     2161 tgcaaatccg ctgccgagaa cccagcatcc gccttcgcgc gcaatgtggc gcatggtcga
     2221 aagccaaggc tcgccgtcgt cataggtaga cgccacatag atctcgacgc cctgcgcgta
     2281 gacggcatag cgcgccagcg gcatgtaatt ctctcagcag atgagcgtcg acagccgccc
     2341 gtgtggcgtt tcgacaaccc tgagaccgga tgcatcgccc atgccccaca ccatgcgttc
     2401 gggattggtg ggcatgagct tgcggtgacg gttcagtatg gttccgtccg gcccgatcac
     2461 gacgatcgtg ttgtacaacg tgccgtggcc gaacctgctc tcgcgttcat tcatcccgca
     2521 agcgattgcg ggaatgacaa caagtggggt tgcaattccg ctatcggaca gtgccctaat
     2581 cttactgagt cagaaaggtg agtcagaaag ccg
//
LOCUS       DASZMC010000456         5124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4860112,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000456 DASZMC010000000
VERSION     DASZMC010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4860112"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..940
                     /locus_tag="VG274_09480"
     CDS             395..940
                     /locus_tag="VG274_09480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE06927.1"
                     /translation="MDELVTAIRIARPEDAAAVARIYIESWHDTYAAILPVRMLCAMT
                     PKGQAARWRAAIRARGREQVLVAESDRHGIVGMSSIGPSRDGGLGYDGEVYTLYVDPA
                     FFGRGAGRALLRAGFASLRTRGFSSCMIWAHARNPVRFFYEAMGGRLVAERVQRLMGD
                     PVPEAAFAWRRLALAERSPAR"
     gene            complement(958..1488)
                     /locus_tag="VG274_09485"
     CDS             complement(958..1488)
                     /locus_tag="VG274_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWE06928.1"
                     /translation="MKRLFILRHAKAGPHDEKHDKERALIGRGRSDCALMGAEMRKKD
                     YLPDLVLCSSARRTTQTWEYAAPEIGAHPKTRFLDSLYDATAAAILKCVRTESGDARA
                     VAVIGHNPGLEDFARKLVRKPANTEERSRAAALAAKFPTCALAVFDFDVDEWAKIAPG
                     TGVLTDYIAPAGLKAR"
     gene            complement(1520..1930)
                     /locus_tag="VG274_09490"
     CDS             complement(1520..1930)
                     /locus_tag="VG274_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HWE06929.1"
                     /translation="MNAISHHAALIYVMVVVSASDGNMSERELRTIGDIVRTLPVFRG
                     FDERRLLAVSQECAAILQEQDGLDAVLGLIKDALPEPLRETAYWLALEVALTEPRVML
                     EEVRIIELLRRSLEIDRLSAAALDHAAEARYRTP"
     gene            2023..3213
                     /gene="metZ"
                     /locus_tag="VG274_09495"
     CDS             2023..3213
                     /gene="metZ"
                     /locus_tag="VG274_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HWE06930.1"
                     /translation="MTTHKKPDGWRPRTLAVRGGQIRTSFQETAEPLFLTSGYAYESA
                     EEAESRFKGAPGFMYTRYGNPTTANFEARIAALEGAPVARATASGMAAVTAAFLCCLR
                     HGDHVIAAKAMFGACRYVIEEVLARFGITHTLVDGEDPAQWAGALRPQTRMFFLETPA
                     NPTLSLVDLRAVCAIAAHAKAKVIVDNAFATPVLQRPMEFGADIVVHSSTKYIDGQGR
                     CLGGMILCGEDFLKEHLQIFLRNTGPSISPFNAWVHLKSLETLGPRMAAHCANAAAVA
                     DFLSAQNTILRVLYPFRQDHPQHELARAQMEGGGGVVAFEIEGGKPAAFRFANSLQLI
                     DISNNLGDTKSLITHPATTTHQRLTAQARAELGITDGMLRLSVGLEDPADLCDDIAGA
                     LRTV"
     gene            3227..3682
                     /locus_tag="VG274_09500"
     CDS             3227..3682
                     /locus_tag="VG274_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWE06931.1"
                     /translation="MSEHRAQLRWKRTSHDFTYESYNRAHEILFKDGAIVLPSSSAPE
                     FRGDPERVDPEEAFVASLSGCHMLTFLAISARKRLTLDAYDDDASGHLEKADGGRLWM
                     ARVTLRPLVRFALGIGVDAQLLAALHHQAHADCFIANSVKTEVLVLPRA"
     gene            3751..4191
                     /gene="apaG"
                     /locus_tag="VG274_09505"
     CDS             3751..4191
                     /gene="apaG"
                     /locus_tag="VG274_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HWE06932.1"
                     /translation="MDQHPSSRPSAPRHQYTYERTTRSIRVAVKPAYLDDQSDPSEGR
                     YVWTYAITIENRGSETVQLLSRYWHITDARGHVQEVRGPGVVGAQPVLEPGQVFEYAS
                     GCPLPTPSGAMVGRYELRAASGETFEADIPMFLLESPYERRQIH"
     gene            4245..4886
                     /gene="folE"
                     /locus_tag="VG274_09510"
     CDS             4245..4886
                     /gene="folE"
                     /locus_tag="VG274_09510"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWE06933.1"
                     /translation="MSELPKLSARSLAPAALGARPSRGEAEQAVRTLLKWAGDDPTRE
                     GLRETPERVARAFEDWFAGYAMDPEEYLARTFEEVEGYDDMVVLRDIRFESHCEHHLA
                     PIIGRVHVGYLPTNKVVGISKLARVVEAYAKRLQVQEKMNAQIARCIQKVLEPKGVAV
                     VIQAEHQCMTTRGVHKPGVSMVTSTMLGEFRKNPLTRREFLSVIGNPASSVAD"
BASE COUNT          906 a         1629 c         1625 g          964 t
ORIGIN      
        1 ctcctgctta gcctctcgct gcctgctgaa gtaatcctca agcatctgcg ttcgttccac
       61 atttgttagc cgctggggca cggcttcccg ttcgcacgaa cctcgtgcgg cgcgatccta
      121 cgaacacggt tttatccgac accgtttcga cggtcctgaa ctcgagcccg gcacaagcaa
      181 atcagcgacg atcgataagc cattgtaact ccttttcctt cggatgccat agcggcgccc
      241 tgaaattcct gtgggcgcca cattcccaac aagattctgc cggtcgcggg aaattccgcc
      301 cagcgcgggc agccggaacc gtgctgtgct gcacccgcac aattcagcgt gaagcgcttg
      361 ccgacatcac cgttcttcgg tcaatctcgt gcggatggac gagcttgtta ccgcgatacg
      421 catcgcgcgg cctgaagatg ccgccgcggt cgcccgaatc tacatcgagt cctggcacga
      481 cacctacgcc gcgatattgc cggttcgcat gctctgcgcg atgacgccga aagggcaggc
      541 ggcgcgatgg cgcgcggcga ttcgcgcgcg cggccgcgaa caggttttgg tggccgagag
      601 cgacaggcac ggcatcgtcg gcatgtcgag catcggccct tcgcgcgacg gcggtctggg
      661 ctacgacgga gaggtctata cgctgtacgt cgatccggcg ttcttcggcc ggggagccgg
      721 gcgcgcattg ctgcgggcag gattcgcgtc gctgcgcacc cgcggctttt cgtcctgcat
      781 gatctgggcg catgcccgca atccggtgcg attcttctac gaggcaatgg gcggacgtct
      841 tgtcgccgaa cgggtccagc gtttgatggg ggatccggtt cccgaagcgg cgttcgcctg
      901 gcgaaggctg gctctcgcag agcgcagccc ggcgaggtag tgcaggagtt aaggctctta
      961 tcgcgctttc aaaccggccg gcgcgatgta atcggtcaga acgcccgttc ccggcgcgat
     1021 ctttgcccat tcgtcgacgt cgaaatcaaa caccgcgagc gcgcaggtcg ggaatttcgc
     1081 agcgagggcc gcggcgcggc tgcgctcttc cgtattggcc ggcttgcgca cgagtttgcg
     1141 cgcgaaatcc tccaagcccg gattgtggcc gatgacggct accgcacgcg cgtctccact
     1201 ctccgtccgg acgcatttca ggattgctgc ggccgtggcg tcgtagagcg aatcgaggaa
     1261 tcgcgtcttg gggtgggcgc cgatttccgg cgccgcgtat tcccaggtct gcgtcgtgcg
     1321 tcgcgcgctc gagcaaagca caaggtcggg gagatagtct ttcttgcgca tctccgcgcc
     1381 catcaacgcg caatcgctgc ggccacgccc gatgagcgcg cgctccttgt cgtgtttttc
     1441 gtcatgaggc ccggctttgg cgtgccgcaa tatgaacaag cgtttcatga aaatgcgctg
     1501 tcgtttcgtt cgatgcgcgt caaggggtcc ggtagcgcgc ctcggccgcg tggtcgagcg
     1561 cggcagcgga aagtctgtcg atttcaagac tgcggcgcaa gagttcgatg atgcggactt
     1621 cctccagcat cacgcgcggt tcggtgagag cgacttcgag cgcgagccag taggccgtct
     1681 cgcgcaaggg ttcgggcaag gcatccttga tcagccccag cacggcgtcg agcccgtcct
     1741 gctcctgcag gatcgcggcg cattcctgcg agacagcaag caggcgacgc tcgtcgaacc
     1801 cgcggaacac cggcaaggtg cggacgatgt ctccgatcgt gcgcaattct cgctcgctca
     1861 tattgccatc ggaagccgag acgacaacca tcacatagat cagcgccgcg tggtggctta
     1921 tggcgttcat gaaggccctc gaatttggcg cgaccttaat gcgccttgac cgcgcggggc
     1981 aaggcgcgtc accaaacggc ccgataacag gtgcacagca tcatgacgac gcacaagaaa
     2041 ccggatggat ggcggccgcg cacacttgcg gtgcgcggcg gccagatccg cacctcgttc
     2101 caggaaaccg cggagccgct ttttctgacc tccggctacg cttacgagtc ggccgaggaa
     2161 gccgagtcgc ggttcaaggg tgcgcccggc ttcatgtata cgcgctacgg caatccgacg
     2221 accgccaatt tcgaagcgcg aatcgcggcg ctggagggag cgccggtcgc gcgcgcgacc
     2281 gcaagcggaa tggcagcggt gaccgcggct tttctctgtt gccttcgcca tggcgatcac
     2341 gtgattgccg ccaaggccat gttcggcgcc tgccgctatg tgatcgagga ggtgctggcg
     2401 cgctttggca tcacgcacac gctggtggac ggcgaggatc ccgcccagtg ggcgggcgcg
     2461 ctgcggcccc aaacccgcat gttcttcctg gagacgcccg cgaatcccac tctttctctt
     2521 gtcgacctcc gtgccgtctg tgcgatcgcc gcgcacgcga aggcgaaagt gattgtcgac
     2581 aacgcgttcg cgactcccgt tctgcagcgg ccgatggagt tcggcgccga cattgtggtc
     2641 cactcctcga cgaaatacat cgatggccag gggcgctgtc tcggcggcat gatcctctgc
     2701 ggcgaggatt tcctgaaaga acatctgcag atttttctgc gcaatacggg gccgtcgata
     2761 agtcccttca acgcctgggt tcatttgaaa agccttgaga cgctcgggcc gcggatggcc
     2821 gcgcattgcg cgaacgcagc cgccgtcgca gatttcctgt ccgcgcagaa tacgatcttg
     2881 cgggtactct acccgtttcg ccaggaccac ccccagcacg aactcgcgcg cgcgcagatg
     2941 gagggaggcg gcggcgtcgt tgccttcgag atcgaaggcg gcaagccggc tgcctttcgc
     3001 ttcgccaatt cgctgcagct gatcgacatt tccaacaacc tcggcgacac gaaaagcctg
     3061 atcacccatc ccgcgacgac gacgcaccag cgcctgacgg ctcaggcccg cgccgaactc
     3121 ggcatcaccg acggaatgtt gcgcctgtcg gtgggtctgg aagacccggc ggacctgtgc
     3181 gacgacattg ccggggccct gcgaaccgtt tgagggaggt ctcgccatgt cggaacatcg
     3241 cgcgcaactt cgctggaagc gcacaagcca cgacttcacc tacgaaagtt ataatcgcgc
     3301 acacgagatc ctgttcaagg atggcgccat cgtgctgccc tcgtcctcgg cgccggaatt
     3361 tcgtggcgat ccggagcgcg tcgatcccga agaagcgttt gtcgcctcgc tgtcggggtg
     3421 ccacatgctg acgtttcttg cgattagcgc gcgcaagcgc ctcacgctcg acgcgtacga
     3481 cgacgatgcg agcggccatc tggaaaaggc ggatggcggc cggctctgga tggcgcgtgt
     3541 gacgctccgg ccgcttgtgc gcttcgccct gggcatcggc gtagacgctc aattgctggc
     3601 ggcgctccac catcaagctc atgcggattg cttcatcgcc aactccgtca agacggaagt
     3661 tctggtattg ccgcgagcct gaggccgcac gttaacgtct cgagcgggaa gttcttgcgc
     3721 ggggcgcctg catgggacga tagtgattcg atggaccagc atccctcaag ccggccctct
     3781 gcgccgcgac atcaatatac gtatgagcgc acgacgcgca gcattcgcgt cgccgtgaag
     3841 cctgcctatc tcgacgatca gtccgaccca tccgaagggc gctacgtctg gacctacgca
     3901 atcacgatcg agaaccgggg ttcggagacc gttcagctcc tttcgcgtta ttggcacatc
     3961 accgacgcgc ggggacacgt ccaggaagtc cgcgggccgg gcgtggtggg cgcgcaaccg
     4021 gtgctcgaac cggggcaggt gttcgaatat gcgagcggtt gcccgttacc gactccctcc
     4081 ggcgccatgg tggggcgcta tgaattgcgc gccgcctcgg gtgagacctt cgaggccgat
     4141 atccccatgt tcctccttga aagtccctac gagcgccgcc aaatccactg aaccccgagt
     4201 cgctcgtgga ctcgctggac ccgcatttcc gtaaagggag aaccatgtcc gaattgccaa
     4261 agctgtctgc gcgatctctc gctccggcgg cgcttggcgc ccggccgtcg cgcggggaag
     4321 ccgagcaggc agtccgtacg ctccttaagt gggcgggaga cgatcccacg cgcgaaggcc
     4381 tgcgggaaac gcccgagcgc gtggcgcgcg cgttcgagga ctggtttgcc ggttacgcca
     4441 tggaccccga agaatatctc gcgcgcacat tcgaagaggt ggaggggtac gacgacatgg
     4501 tcgtgctgcg cgacatccgc ttcgaatcgc attgcgaaca tcatctggcg ccgatcatcg
     4561 gccgcgttca tgtaggttat ttgccgacca acaaggtcgt tggcatctcg aagctggcgc
     4621 gcgtcgtcga ggcctatgca aagcggctgc aggtgcagga gaagatgaac gcccagatcg
     4681 cgcgctgcat ccagaaggtg ctcgagccca agggcgttgc ggtcgtcatc caggccgagc
     4741 accaatgcat gacgacgcgc ggcgtgcaca agcccggcgt gagcatggtg acctcgacga
     4801 tgctgggcga attccgcaag aatcccctca cgcggcggga gttcctgagc gtcattggca
     4861 atccggcatc gagcgtcgcc gactgagggc ggggagagcc ccggtggttc agttccaaca
     4921 attgaatccc caaaaattgt catggccccg tttaacgggg ccacccagcc agcgcgcgtc
     4981 cgcgcgctga atatacgctc tgggcggttt cgtctgggtg gcccgctctg cggcgggcca
     5041 tgacaaattt ttgctaccaa gtgttggaac cggtacatca gccaaggtcc agtggctcga
     5101 cgcgctcggc gcccttcgcg ccgg
//
LOCUS       DASZMC010000457         9114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5412006,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000457 DASZMC010000000
VERSION     DASZMC010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5412006"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..945
                     /locus_tag="VG274_09515"
     CDS             106..945
                     /locus_tag="VG274_09515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HWE06934.1"
                     /translation="MAGKPNISLLLADVDGTLVTRDKVLTPRARQAVIRLRERNIHFA
                     ITSGRPPRGMAMFVDPLGLVEPIAGFNGGVYVAPDLRVLESHGLSPDAARKAVELLAG
                     HGLDAWLYTGKDWYVRNPDAPHVARETRTVTFAPSVAETFDSFYSEAVKVVGVSDDQA
                     RMRWASDELNRVLGPDASVSLSQSYYIDITNPIANKAAVLRYHASRLGIAASAIATIG
                     DMPNDVLMFGASGFSISMGNAAPDVQAEATVVTDSCDDDGFAKAVERYLLDGQPPAST
                     DSS"
     gene            1084..2184
                     /locus_tag="VG274_09520"
     CDS             1084..2184
                     /locus_tag="VG274_09520"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWE06935.1"
                     /translation="MAPEEAGRSASNSGGATAHQGEESPNLFQRPAVLAGGGILLAVL
                     IVAGLVWWLIARQYEDTDDAFIDTHVVHVAPQIAGRIARIFVNDNQLVEAGQLLVEID
                     PSDETARLNQAQAQEVQSETQLNQARAQVGVSEAAFDQASASARGAEAQAVNAARDLA
                     RYRALKASTPLAVAQEQLDTAIATARNTAAAHDAASQQVEGAKAQIVAVKAQLSGAQA
                     SVNAFKAEFAQAKLNLGYTRIAAPVAGHIAQRSVAIGNYVQSGEQLMAVVPLKIWVTA
                     NFKETQLDRMRPGETVDIEVDACPSASVRGHVDSIQRGAGQAFGLLPPENATGNYVKV
                     VQRVPVKIVLDSVPDNCPLGPGMSVVPSVKVR"
     gene            2286..3824
                     /locus_tag="VG274_09525"
     CDS             2286..3824
                     /locus_tag="VG274_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWE06936.1"
                     /translation="MVGVISIATFMVVLDTSIANVALAHIAGSLSASYDEATWVVTSF
                     LVANAVIIPISGWLADVIGRKRFYMISVALFTVSSFFCGLAPSLGLLVIARVLQGIGG
                     GGLTPVEQSMLVDTFPPEKRGLAFAAYGVVVIVGPILGPSIGGWITDNASWHWVFLIN
                     VPVGILSLFLTSIFVDEPKALREQRHDLLKDGLKVDFIGFALEALFLGCLEVTLDRGQ
                     REDWFSSGLITAFAIIAALSLAIFIPWELTRKDPIVRLSMFGCRNFGIASLLMLVMGM
                     VIFSSTQFIPQLLQEVLGYTATDAGFALTAGGVATFLVMPLGGVLSSRVDPRFLIGGA
                     FLLQSFALWHMSHLNALMSFNDAAFARMLQSIGLPFLFIPISSIAYVGLKPEENNQAS
                     ALLNMARNLGGTIGISAVQTMLARRSQIHQAQYVETLNPLNPNYQYGTQQIIDALVAR
                     GQSQWEATRQASAELYRELVQQAAILSYIDTFHVLMIVVLCAVPLAFLLKKPGKQPAA
                     EAVP"
     gene            3821..5305
                     /locus_tag="VG274_09530"
     CDS             3821..5305
                     /locus_tag="VG274_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWE06937.1"
                     /translation="MTRALAVACGLPLLLAACNVGPDYRKPEIPVPARFSEAGSAVPA
                     VAQSDLSKWWTQFQDPVLDALVARALSANLDLQTAASRVRQAREQVIVAGAAEWPTLN
                     ANGLGLNLHSNSSPLSQLASAGAPPGTAPSATPTNIKLYSLGFDATWELDVFGGTRRA
                     VEAAQANSEAALWELHDGEVTLTAEIANDYLGLRAAQARIALLEEELKDQRQMLDLVR
                     ARAKNGFVTQLDVNQQRALASSTEAQIPDLDAQSRATMHALAVLLAEPPESVDSALQE
                     AQPMPAAPLAIPASLPSDLLRRRPDIRDSERKLAAATANEGAAIAALYPKFNLIGAAT
                     FAGSSLSGLLSTDNFGTVGIGSIMWPIFEGGKIHANIRSSEEAELQAYLAYRKSVLGA
                     LQNVEDALARYADDGRHVRALEDAESAAASSAAIAQQQYANGLVNFITVLSTETTLLS
                     ARDQLIQARQNLAQDFGALYKALGGGWDEASVDWQTRPSEPENR"
     gene            complement(5322..5537)
                     /locus_tag="VG274_09535"
     CDS             complement(5322..5537)
                     /locus_tag="VG274_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06938.1"
                     /translation="MSAGVTYRFDLLNDSGTRNCLFIAQCFGDEHAVLLARALFESTF
                     VRMKSIRVWRDDVLVHEANKSKKAKIN"
     gene            complement(5701..7980)
                     /locus_tag="VG274_09540"
     CDS             complement(5701..7980)
                     /locus_tag="VG274_09540"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWE06939.1"
                     /translation="MAQSARAAIGGGAPRIDGRLKVTGDAPYGSDFMTPSAPVFAFLR
                     TSSIARGHIADIDEREARGLPGVLDILTFKTVGSAIKPGNVFDKGGYLGTSIAPLASA
                     EIRHDGQIVAVVLAETFEAARDGAHRLRIAYVEERPAATFGSPGTVSAAASAVSKSHK
                     DPMVGDAERAFARAPVTIEAQYSTPTQHHNPMELFATTCAWSNGKLTVWESSQNVWGF
                     KNGIADQLGIASDDVLVISPYVGGAFGSRGSLTQRTALIALASRRIGRPVKLVCSRSD
                     GFTIGTYRAETRHRFRLGADREGHLTALSHEGWEVTSRPDNYMVAGTDASTRLYACAN
                     VASRVSIVHADRNTPGFMRSPPETPYIFPLESAMDELSYALGIDPVELRRRNDTMREP
                     IKGLLYTSRSLMKCFDEGARQFQWERRNPKPASMRDGDWLIGQGCAATMYPTVMGAAT
                     ARVILSPDARVRVQTAAHDLGTGAYTAIALVAADALGVNLSDVTVELGRSDLPPAPVA
                     GGSNTTASVCNVVAKACGEIRQRLQRTAAANGPLAGRDRGGFVFADRTIRGPDGASER
                     LEPVVARAANGAIEVYAENIPHDVSPEAIGKLYKGHPTLAGGAHMKDRIQFAFGADFV
                     EVRVHARTREIRVPRIVGAFAAGRIVNPVTARSNLMGGLIWGVSAVLHEATEIDARAA
                     RYTNKDLAEYMIPVNADIEDLEVILVPEEDTKVNPLGIKGLGELGNVGVNAAVANAVY
                     HATGIRIRDLPIRLEKLLA"
     gene            complement(7983..8996)
                     /locus_tag="VG274_09545"
     CDS             complement(7983..8996)
                     /locus_tag="VG274_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWE06940.1"
                     /translation="MRPFTLHRVSDAAAAISAARSPVAAPTLAPIQYLAGGTTLLDLM
                     KLDVMRPAEVVDINDLVRDFGYIEPRTDGLHLGALARMAEVADEARIRRDYPVIAQSL
                     SLAASAQLRNMATLGGNVLQRTRCSYFRDSSWRECNKRDPGSGCAALDGVNRKHAILG
                     TSAHCIATYPGDFANALVALDGQVEILGRQGARRIAMENLHREPGGTPHIETVLAPGD
                     LITGFFVPAAPWTRRSLYLKIRDRQSYEFALAGAAVALDLQDGIVRAVRIALSGVATR
                     PWRAREAESALVGRALNPHTAREAAAAAFAQSVTHGGNAYKPELGRRVVERALFEAAA
                     LEA"
BASE COUNT         1637 a         2936 c         2836 g         1705 t
ORIGIN      
        1 gcagacggaa tcgtcacagc ggtcgcagcc tgctcggctg tgctgtagaa atcctcgtct
       61 ggacgttttg gaacggcacc acgtcccgcc caatggagga ttccgatggc cggaaagccc
      121 aacatttccc ttctgctcgc cgacgtggac ggaaccctgg tcacgcggga caaggtcctt
      181 acccccaggg cgcggcaggc cgtcatccgc cttcgcgagc ggaatatcca tttcgcaatc
      241 acctcgggac gcccgccgcg cggcatggcg atgttcgtcg atccgctggg actcgtcgag
      301 ccgattgcag gcttcaacgg cggcgtgtac gtcgcgccgg acttgcgcgt tttggaaagt
      361 cacgggctgt cgccggatgc ggcgcgcaag gcggtcgagc tactcgccgg acacggactc
      421 gatgcctggc tttatacggg caaggactgg tacgtgcgca atcccgatgc gcctcacgtc
      481 gcgcgcgaga cgcgcacggt caccttcgca ccgagcgtcg cagaaacctt cgactcgttt
      541 tattcggagg cggtcaaggt tgtaggggta agtgacgacc aggcgcgcat gcgatgggcc
      601 agcgacgaac tcaaccgcgt gctggggcca gacgcctcgg tgtcactgtc acaatcctat
      661 tatatcgaca tcaccaatcc gatcgccaac aaggcggctg tcctgcgtta tcacgcgagc
      721 cggctcggca tagccgcatc ggcgatcgcc acaatcggag acatgccgaa cgatgtgctg
      781 atgttcgggg caagcggatt ttccatctca atgggcaacg ccgctcccga cgtgcaggca
      841 gaagcgaccg tggtgacgga ttcctgcgac gatgatggtt tcgccaaggc ggtggagcga
      901 tatctgctcg acggccagcc cccggcctcc accgattcca gctgacgcgg cgttcggcag
      961 cggtctgttg ccgccggaca ccatgtggat cgttggtctg gacggaacca agccgaccct
     1021 cgtgcgtcta atcttggccg tctctatcat ttggcaatcc gcgggatgcg accggagtga
     1081 gccatggcgc ctgaagaggc tggccggagt gcgagcaata gtggcggagc gacggcccat
     1141 cagggagaag aaagtcccaa tctcttccag cggcccgccg ttctcgcagg cggcggcata
     1201 ctccttgcgg ttctcatcgt agccggtctc gtctggtggc tcattgcgcg ccagtacgaa
     1261 gacacggacg acgctttcat cgatacccat gtcgtgcatg tggcgccgca gatcgccggc
     1321 cggatcgcgc gcatcttcgt caacgacaat cagctcgtcg aagcgggaca gctattggtc
     1381 gagatagatc catcggatga gaccgcgcgg ctcaatcagg cgcaggccca ggaggtacag
     1441 tccgaaacac aactcaacca ggcgcgcgcg caagtcggcg tgagcgaagc cgctttcgat
     1501 caggcgagcg ccagcgcgcg aggcgccgag gcgcaggccg tgaatgccgc ccgcgacctt
     1561 gcacgctatc gcgcattgaa agcgtcgaca ccgcttgcgg tcgcccagga gcagctcgac
     1621 accgccattg ccacggcacg aaacaccgct gccgcgcatg acgcggcaag tcagcaggtc
     1681 gagggcgcca aggcccagat tgtcgcggtc aaggcgcagc tctcgggtgc gcaggcaagt
     1741 gtgaacgcat tcaaggcgga gtttgcgcag gccaaactca accttggcta cacgcgaatc
     1801 gcggcgcccg tcgcaggcca tatcgcgcag cgcagcgttg ccattggcaa ttacgtgcag
     1861 tcgggcgagc agctgatggc agtggtgccg ctcaagatat gggtcacggc gaacttcaag
     1921 gagactcaac tcgaccggat gcgtccgggc gagaccgtcg atatcgaagt ggacgcgtgt
     1981 ccctcggctt cggtgcgcgg tcacgtcgat tcgattcagc gcggcgcggg ccaggctttc
     2041 ggcctgctgc cgcccgagaa cgccacgggc aactacgtca aggtggtgca gcgcgtaccg
     2101 gtcaagattg ttctcgacag cgtgccggac aattgccctc tcgggcccgg catgtcggtc
     2161 gttccgtccg tcaaggtgcg ctagtgtccg gagctggcgc gagcgggacc caacgcgcag
     2221 ctggcggttc atccgcggga tggacgcccg cacgctccgt cgccggcggc cgcaatccct
     2281 gggtcgtggt cggcgtcatt tcgatcgcca ccttcatggt ggtgctcgat acgtcgatcg
     2341 ccaacgtcgc gctcgcccac atcgccggca gcctgtccgc aagctacgac gaagcgacgt
     2401 gggtagtaac gagctttctc gtcgccaacg cggtgatcat tccgatcagc ggctggctcg
     2461 ccgacgtcat cggccgcaag cgcttctaca tgatctcggt tgcgctgttc accgtgtcgt
     2521 cgttcttctg cgggctggct ccgagtctcg gactcctcgt catcgcccgc gtgctccagg
     2581 gcatcggcgg cggaggcttg acgccggtcg aacaatcgat gctcgtcgat acattcccgc
     2641 cggagaagcg cgggctcgcc ttcgcggcct acggagttgt cgtcatcgtg ggccccattc
     2701 tcggtccgag catcggcggc tggatcaccg acaacgcctc gtggcactgg gtattcctga
     2761 tcaacgttcc ggtggggatt ctctctcttt tcctgacatc cattttcgtc gacgagccca
     2821 aagcgctgcg cgagcagcgc cacgatctcc tcaaggacgg cctcaaggtc gatttcatcg
     2881 gcttcgcact tgaagcgctg ttccttggtt gcctcgaagt gacgctcgac cgcggccagc
     2941 gcgaagactg gttctccagc gggctgatca ccgccttcgc gatcattgcc gcactttctc
     3001 ttgccatctt cataccgtgg gagctcacgc gcaaggatcc gatcgtgcgc ctgtcgatgt
     3061 tcggctgccg caatttcggc attgcgagcc tgctcatgct cgtgatggga atggtcatct
     3121 tttcgagcac gcagttcatt ccccagctcc tgcaggaggt gttgggatat accgccaccg
     3181 acgcgggatt cgcgctaacg gcgggaggcg tcgccacatt tctcgtcatg ccgctgggcg
     3241 gtgtcctgtc gagcagggtc gatccgcgct ttctcatcgg cggcgccttt ctgctgcaga
     3301 gcttcgcgct ctggcacatg agccatctca atgcgctgat gtccttcaat gacgccgcct
     3361 ttgcgcggat gctgcaatcg atcggtctgc cgtttctgtt cattcccatc agcagcatcg
     3421 cctatgtcgg attgaagccc gaggagaaca accaggcgtc cgcgcttctg aacatggcgc
     3481 gcaatctggg agggacgatc ggcatctcgg ccgtccagac aatgctggcg cggcgctcgc
     3541 agattcatca ggcgcaatat gtcgagacgc tcaatccgct caatccgaac tatcaatacg
     3601 gcacacagca gatcatcgac gccctcgttg cgcgcggaca gtcgcaatgg gaggccacac
     3661 ggcaggccag tgcagaactg taccgcgaac tcgtgcaaca ggccgcgata ctctcctaca
     3721 tcgatacatt tcatgtcctg atgattgtgg tcttgtgcgc ggtgccgctc gcgttcctgc
     3781 tgaaaaaacc cgggaagcag ccggccgcgg aggccgtgcc gtgacccgcg cacttgccgt
     3841 tgcgtgcggc ctgcccctgc ttctcgccgc gtgcaatgtt ggcccggact acagaaaacc
     3901 cgaaattccc gtacccgcca ggttttccga agccggaagc gcggtccctg ccgtcgcgca
     3961 atccgatttg tccaaatggt ggacgcagtt tcaggatcct gtgctcgatg ctctcgttgc
     4021 gcgcgcgctt tccgccaatc tcgacctgca gaccgccgcg tcgcgcgttc gccaggcgcg
     4081 cgaacaggtg atcgtcgcgg gcgccgccga gtggccaacc ctgaacgcca atggtctcgg
     4141 actgaacctc cattccaact ccagcccgct ctcgcagctg gccagcgcag gcgcaccgcc
     4201 cggaaccgcg ccctccgcca cgccgacgaa tatcaagctc tactcattgg gattcgatgc
     4261 gacgtgggag ctcgacgtct tcggcggaac gcggcgtgct gtggaggctg cacaggcgaa
     4321 tagcgaggcg gcgctatggg agctgcatga cggcgaggtg acgctcaccg ccgagatcgc
     4381 caacgattat ctcggcctgc gcgcagcgca ggcacggatc gctctgctcg aagaagaact
     4441 gaaagaccag cgccagatgc tcgatctcgt tcgcgccaga gcgaagaacg gtttcgtaac
     4501 ccagctcgac gtgaatcagc agcgcgcgct tgcatcgtca accgaagcgc aaatcccgga
     4561 cctcgatgcg cagtcgcgcg cgacaatgca cgcgctggca gtgctgctcg cggaacctcc
     4621 ggagtcggtg gatagcgcgt tgcaagaagc gcagccgatg cccgccgcac ctcttgccat
     4681 tcctgcctcc ctgccgtcgg atcttctccg ccgcaggccc gacattcgcg actcggaacg
     4741 caagctcgcg gcggcgacgg cgaatgaggg cgcagcaatc gccgctctct atcccaaatt
     4801 caatctcatc ggtgctgcga cctttgccgg aagcagtctg agcgggctgc tatcgaccga
     4861 caatttcgga accgttggaa taggttcgat catgtggccc atctttgaag gcggaaaaat
     4921 ccacgcgaac attcgttcga gcgaagaggc ggagctgcag gcctaccttg cctatcgcaa
     4981 atccgtgctt ggcgcgttgc agaacgtgga ggatgcgctg gcgcgatatg ccgatgacgg
     5041 ccggcacgtg cgggcactcg aagacgccga gagtgcggcc gcgtcctcgg cggcaatcgc
     5101 gcagcagcaa tacgcgaacg gtcttgtcaa tttcatcacc gtgctgagca cggagacgac
     5161 gctgctctct gcgcgcgatc agctcatcca ggcccggcaa aacctcgccc aggatttcgg
     5221 agcactctac aaggcgctcg gcggcggttg ggacgaggcg tcggtcgact ggcagacccg
     5281 cccatccgaa ccggaaaacc gctgattgcg gcgccgcgcg ttcaattgat cttggccttc
     5341 ttcgatttgt tggcctcgtg aacgagcacg tcgtcgcgcc acaccctgat gcttttcatg
     5401 cggacgaacg tgctctcgaa caacgcgcgg gcgagcagca ccgcgtgttc gtcgccgaaa
     5461 cattgcgcaa taaaaagaca attgcgcgtg ccgctgtcgt tgagcagatc gaaccggtac
     5521 gtcacgcccg cactcatgga cgtcctccaa cccaaccgcg cgagagcccc gactggcggt
     5581 caatgcgtac attttccatg caggcaatcg tgatgtcgag cggaattttc acactttgcg
     5641 atgcggttct gttcgccagc gccgccgccg tccagacgca tgcggcgcat caaagataat
     5701 ttacgccaac agcttttcga gccggatggg caagtcgcgg atgcgaatgc cggtggcgtg
     5761 atagaccgcg ttggcgacag ccgcgttaac acccacattg cccagctcgc cgagcccctt
     5821 gatgccaagc ggattcactt ttgtgtcctc ttccggcacg aggatcactt ccaggtcttc
     5881 aatgtctgcg ttcaccggta tcatgtactc cgcaagatcc ttgttggtat aacgcgccgc
     5941 gcgagcgtcg atctcggtcg cttcgtgcag gacggcggag acgccccaga tgagaccgcc
     6001 catcaggttg ctcctggccg tcacgggatt gacgatccgc ccggccgcga acgcgccgac
     6061 gatccgggga acgcgaattt cgcgcgtccg cgcgtgaacc cgcacttcga cgaaatctgc
     6121 gccaaatgcg aactggatgc ggtccttcat atgggcgccg cccgcgagtg tgggatggcc
     6181 cttgtagagt ttcccgatcg cctcgggaga aacatcgtgc ggaatgtttt ccgcgtagac
     6241 ttcgatggcg ccattggcgg ctcgcgcgac gaccggttcg agcctttcgc tggcgccgtc
     6301 cgggccgcgg atcgtgcggt cggcgaagac aaagccaccg cgatcgcgtc cggcgagcgg
     6361 tccgttcgcg gcggccgtcc gctgcaggcg ctgcctgatt tctccgcacg ccttggccac
     6421 gacgttgcag acgctggccg tcgtgttgga cccgccggca accggcgccg gcggcaaatc
     6481 ggagcggccg agctcgaccg tcacatcgga gagattgacg cccaacgcat cagcggcgac
     6541 aagcgcaatc gcggtatagg cacctgtgcc cagatcgtgg gctgcagtct gcactctcac
     6601 gcgtgcgtcc ggcgacagga tcactcgcgc tgtggcggcg cccatgaccg tcgggtacat
     6661 cgtggccgca caaccctggc caatgagcca gtcgccgtcg cgcatggaag cgggttttgg
     6721 attgcgcctt tcccattgaa attgccgcgc gccctcgtcg aagcatttca tgagggagcg
     6781 gctggtgtac agcaatccct tgatcggctc gcgcatcgtg tcgttgcggc ggcgcaattc
     6841 gactggatcg atgcccagtg cgtaactcag ctcgtccatt gcgctctcga gcgggaaaat
     6901 atagggcgtc tccggcggcg agcgcatgaa gcccggtgta ttccggtccg cgtgcacaat
     6961 gctcaccctg gacgcgacat tggcgcaggc gtagagccgc gtcgaagcgt cggttcccgc
     7021 gaccatgtaa ttgtccggcc ggctggtcac ttcccaaccc tcatgagaaa gagcggtcag
     7081 gtgtccctcg cggtccgcgc cgagccgaaa gcgatgacgc gtctcggcgc ggtaggtgcc
     7141 gatggtgaac ccatcgctgc gcgagcacac gagcttgacc ggcctgccga tacgtctgga
     7201 agcgagtgct atgagggcgg tcctctgcgt gagcgatccg cgcgaaccga aggcgccgcc
     7261 cacgtagggt gaaatgacca ggacatcgtc ggacgcgatt cccagctgat cggcaattcc
     7321 gttcttgaat ccccatacgt tctggctcga ttcccacacc gtcagcttgc cgttcgacca
     7381 cgcgcaggtg gtcgcgaaca gctccatcgg attgtggtgt tgcgtgggcg tcgaatactg
     7441 cgcctcgatc gtgaccggcg cccttgcaaa ggcgcgttcc gcgtctccta ccatcgggtc
     7501 cttgtgcgat tttgatactg cactggccgc cgcagagacc gtgccggggc tgccgaatgt
     7561 ggcggctggc ctttcctcga cataagcaat ccggagacgg tgtgcgccgt cgcgtgccgc
     7621 ttcgaatgtc tcggccagca cgaccgcaac gatctgaccg tcatgtcgga tttcggcgga
     7681 agcaagtggc gcgatcgacg tgccgagata tccgcctttg tcgaaaacat tcccgggctt
     7741 gattgcgcta cccaccgtct tgaaggtgag gatatcgagg acccccggaa ggccgcgcgc
     7801 ctctcgttcg tcgatatccg cgatgtgtcc acgggcgatc gagcttgtac gaaggaacgc
     7861 gaagaccggc gcgctcggtg tcatgaagtc cgaaccgtac ggcgcgtcgc cggtaacttt
     7921 cagccgcccg tcgatgcgcg gagcgccgcc tccaattgca gcacgtgcgg attgcgccat
     7981 cttcacgcct cgagagctgc ggcttcgaac agagcccgct caacgacgcg gcgcccaagt
     8041 tcgggcttgt aggcgttccc gccatgcgtc acggattggg cgaacgccgc agcggcggct
     8101 tctcgcgccg tgtgcggatt cagcgcgcgg ccgaccagcg cggattccgc ctcgcgagcc
     8161 cgccacggcc tggtcgcaac tccggaaagc gcgatgcgca ccgcgcgcac gatgccatct
     8221 tgcagatcga gggcgacggc cgctcccgca agggcgaact cataggattg acggtcgcga
     8281 atcttcaggt agagcgatcg tcgcgtccat ggcgccgccg gcacgaaaaa gccggtgatc
     8341 aggtcgccag gcgcgagcac cgtttcaatg tgcggtgtac cgccgggttc gcggtgcaga
     8401 ttctccattg cgatcctccg ggcgccctgc ctgccaagaa tttcaacctg gccatccagg
     8461 gcgacaagtg cgttggcgaa atcgccagga taggtcgcaa tgcaatgcgc gctcgtgccg
     8521 agtatggcgt gcttgcggtt cacgccgtcc agagctgcgc atcccgagcc aggatcccgc
     8581 ttgttgcatt cccgccagga agaatcgcga aaatagctgc atcgcgttcg ctgcagcaca
     8641 ttgccgccga gcgtcgccat attgcggagc tgcgcgctcg ccgccagaga cagcgactgc
     8701 gcgatgacag gatagtcgcg gcgaatccgc gcctcgtcgg cgacctcggc catacgcgcg
     8761 agcgccccca gatgcaggcc gtccgttcgt ggttcgatgt agccgaaatc gcgaacaaga
     8821 tcgttgatgt cgaccacctc tgcaggtcgc atgacgtcga gcttcatcag atcgagaagc
     8881 gtcgtgcctc ctgcgaggta ctggatcggc gcaagagtgg gcgctgccac ggggctgcgc
     8941 gcggccgata tggcagccgc cgcgtccgaa actcgatgaa gcgtgaaagg ccgcacggct
     9001 catcccatcc gcgaggccgc ctgcctgacc gcctgcacga tatggggata ggcagcgcag
     9061 cggcacagat tgccgctcat atattcgcgg atgtcgctct cggatttcgc gtgc
//
LOCUS       DASZMC010000458         4374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2665236,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000458 DASZMC010000000
VERSION     DASZMC010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2665236"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VG274_09550"
     CDS             complement(<1..443)
                     /locus_tag="VG274_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWE06941.1"
                     /translation="MPHFVPSLSLSPQLQLLLVDNAINFVAAVVILIAGWTVAGWAAR
                     AVRGGLRRIPRFDATLRPLAGSVVRYTIVILTVIAVLQRFGVETTSLIAILGAAGLAV
                     GLALQGTLSNVAAGVMLIFLRPFRRGEKIECDKCIGVVRELGLFRT"
     gene            complement(452..1300)
                     /gene="lpxC"
                     /locus_tag="VG274_09555"
     CDS             complement(452..1300)
                     /gene="lpxC"
                     /locus_tag="VG274_09555"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HWE06942.1"
                     /translation="MTRRSILREVSCDGVALHAGSSVRARLLPAPAGAGVVFRRNDLG
                     GREIPALYDRVGETRLGTVIAENGATVAVIEHLMAAVAGAGVDDLIVALDGPEPPILD
                     GDALSWLLLLEQAGFRDQPGTRGAIRIARQVEVAQGGASAALVPAEALDFDFDISFPS
                     AAIGTQRLLWAFSGEAFRRDIAPARTFGFVNELDALRGAGLARGASLENTLAIDGDTM
                     VNPDLQRFPDEFVRHKILDAIGDLALAGVHIIGRFVGRRSGHALNNALLRALFSDPVN
                     YELIET"
     gene            complement(1394..2902)
                     /gene="ftsZ"
                     /locus_tag="VG274_09560"
     CDS             complement(1394..2902)
                     /gene="ftsZ"
                     /locus_tag="VG274_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWE06943.1"
                     /translation="MSINLRIPEVTELRPRITVLGVGGAGGNAVNNMIASKLEGVEFI
                     VANTDAQALAQSRAERRIQIGARVTEGLGAGARPDIGRAGAEESLEEILEHLSGSHMV
                     FITAGMGGGTGTGAAPVIARAIREQGVLTVGVVTKPFAFEGDKRMRVAERGIDELQAY
                     VDTLIIIPNQNLFRIANDRTTFSQAFAMADDVLHSGVRGVTDLIVMPGLINLDFADIK
                     TVMSEMGKAMMGTGEADGEERALKAAEAAISNPLLDDVSMKGARGVLINITGGPDMML
                     FEVDQAANRIRAEVDPDANIIFGGTILENMEGRLRVSVVATGIEAEQIAHMPDNVEQL
                     RPRARAAQLGAKLEPRAAANPVRSQMDVLAQEMNASIAPRQSISPVEPIILGTPEVQA
                     IEDEEIPAPEPVRIQPLPRAAEARPAPEPQEEKKRRFGFLGRRKAEPVRAEPQMASGS
                     PHGQRATAQLITREAPGPRPAAPSQANAEDLFPDFKKDDQFEIPAFLRRQTN"
     gene            complement(3057..>4374)
                     /gene="ftsA"
                     /locus_tag="VG274_09565"
     CDS             complement(3057..>4374)
                     /gene="ftsA"
                     /locus_tag="VG274_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWE06944.1"
                     /translation="QRAEAANAGKRGIMGETIRMRTSDAQSPAYRTGLVAALDVGSSK
                     VVCLIGRAEPASLRVIGASLRESRGIKAGTVTSLEHVEESIREAVAAAENLADLRVQH
                     VLLSVNCGAPQSVTARAAMSLEGALVSDEYLLDLLAEGRANCRLDGHEIIQCAPVSYV
                     VDEARGVRDPSGMFCQRIGVQMHAVAVRPSPLQNLKLAVERCHLSIAGALFAPYASGL
                     ATLTPDEMQLGATVIDMGGGVTSIAVFLEGALVHADVVPMGGAYVTADIARMLASPLS
                     AAERIKTLYGAALGDIETGTDVVAVPQMGENGQDCDLRVPRSMLTRIIQPRLEEIFGE
                     VQNRLRASGFDVAAGRRAVLTGGACQLAGARELVQRILNKQVRIGRPHGLAGLPAASA
                     GPDYATAVGLLMAGATMRPEALNPDLAPTLPARQSFLSRITGGLIR"
BASE COUNT          784 a         1446 c         1392 g          752 t
ORIGIN      
        1 gtgcgaaaca gcccgagttc gcgaacgact ccgatgcatt tgtcgcactc gatcttctcg
       61 ccgcggcgga acggcctgag gaagatgagc atgacgccgg ccgccacgtt ggacagcgtg
      121 ccttgcaggg ccagtccgac cgcgagtccg gcggcgccca ggattgcgat gagactggtc
      181 gtttcgacgc cgaaccgctg caacaccgcg atgacggtga gaatgacgat cgtgtatcgc
      241 accacggatc cggccagcgg ccgcagcgtg gcgtcgaacc gcggaatgcg gcgaagaccg
      301 ccgcgaaccg cgcgtgccgc ccaacccgca accgtccagc ccgcaatgag aatgacgact
      361 gcagcaacga aattgatcgc attgtctacg agcaggagct gcaattgcgg cgacagcgac
      421 aggctcggca cgaaatgggg cacgcagaca cctatgtttc gatcaattcg taattcacgg
      481 ggtcggagaa aagcgcgcgc aacagcgcat tgttaagcgc atggccggat cttcggccca
      541 cgaagcggcc gataatgtgc acaccggcga gcgcgaggtc gccgatcgcg tcgagaatct
      601 tgtggcggac aaactcgtcg gggaagcgct gaagatccgg attgaccatg gtgtcgccgt
      661 cgatggcgag cgtgttctcg agggaggcgc cacgcgcgag acccgctccc cgcagcgcat
      721 ccaactcgtt cacgaagccg aatgtgcgcg ccggcgcaat gtcgcgacgg aacgcctcgc
      781 cggaaaaagc ccaaagcagg cgctgcgtgc cgatcgcagc cgaggggaag gaaatatcga
      841 aatcgaaatc gagcgcctcg gcgggaacga gcgccgcgct tgccccgccc tgtgcaacct
      901 cgacctgtct cgcaattctg atcgcgccgc gcgttccggg ctggtcgcgg aaaccggcct
      961 gctcgagcaa caacagccag gagagcgcat cgccgtccag aattggcggc tccggaccgt
     1021 caagcgcaac gatgagatcg tcgacgcctg cgcccgcgac ggctgccatc aggtgttcga
     1081 tgacagcgac ggttgcgccg ttttccgcga tgacggtacc aaggcgcgtt tcgccgaccc
     1141 ggtcatagag tgccggaatt tccctgccgc caagatcatt gcggcgaaag acgactcccg
     1201 cgccagccgg tgcaggcaaa agccttgcgc gcacgctgga acccgcatgc agtgcgaccc
     1261 cgtcgcacga aacctcccgg aggatggagc gccttgtcat tcaccacaaa gacacgaagt
     1321 acacgacgac gaattccagg ctatcttgtc cttcgtgtcc cggtggtgaa atacttcaaa
     1381 ctcccgattg ccgtcaattc gtctgtctgc ggaggaaagc cgggatttcg aactgatcgt
     1441 ccttcttgaa gtcgggaaac agatcttcgg catttgcctg cgacggcgcc gccggacgcg
     1501 gcccaggcgc ttcgcgcgtg atcagctgag cggtcgcgcg ctggccgtgc ggcgatccgg
     1561 acgccatctg gggctcggcg cgcaccggtt cggcctttcg ccgacccagg aatccgaagc
     1621 ggcgcttctt ctcctcctga ggctccggcg ccgggcgcgc ttcggcggcc cgcggcagag
     1681 gctggatgcg aacgggctcc ggcgcaggaa tctcttcgtc ctcgatcgcc tgcacttccg
     1741 gcgttcccag aatgatcggc tcgaccggag aaatcgactg tctgggagcg atgctcgcat
     1801 tcatctcctg cgccagcacg tccatctggc tgcgcacagg gttggccgcg gcccgtggct
     1861 cgagtttcgc gccaagctgg gctgcgcgtg cgcggggccg cagctgctcg acattgtccg
     1921 gcatgtgcgc gatctgctcg gcctcgatgc cggtcgccac caccgataca cgcagccggc
     1981 cttccatgtt ctccaggatc gtgccgccga agatgatatt ggcatccgga tccacctcgg
     2041 cacggatacg gttcgccgcc tggtcgacct cgaacagcat catgtcgggg ccgccggtga
     2101 tgttgatgag cacgccgcgg gcgcctttca tcgacacgtc gtcgaggagc ggattggaga
     2161 tggcagcctc tgcggcctta agcgcgcgct cctcgccatc ggcctcgccc gtgcccatca
     2221 tggccttccc catctccgac atcaccgtct tgatatcggc gaaatcgaga ttgatcagac
     2281 ctggcatgac gatcagatcg gtcacgccgc gcacgcccga atgcagcacg tcgtcggcca
     2341 tggcgaaggc ctggctgaac gtggtgcggt cattggcgat gcggaacaga ttctggttcg
     2401 ggatgatgat cagcgtatcg acataggcct gcagctcgtc gatgccgcgc tcggcgacgc
     2461 gcatccgctt gtcgccttca aaggcgaacg gcttggtgac gacgccgacc gtgagcacgc
     2521 cctgctcgcg gatcgcgcgc gcaatgacgg gagcggcgcc cgtgccagtt cctccaccca
     2581 tgcccgccgt gatgaacacc atgtgcgagc cggaaagatg ctcgagaatc tcctcgagtg
     2641 actcctccgc gcccgcgcgg ccgatatcgg gccgggcacc ggcgcccagt ccttccgtga
     2701 cgcgcgcgcc gatctggatg cgccgttccg ctctcgactg ggcgagagcc tgcgcatcgg
     2761 tgttcgcgac gatgaattcc acgccttcga gtttcgaggc gatcatattg ttgacggcat
     2821 ttcctccggc gccgcccacg ccaagcacgg tgatgcgcgg ccgcagctcg gtgacttccg
     2881 gaattctcaa atttatcgac attttccgct ctccttgtcg tgtgcgacca tggtcctcac
     2941 cgaacgccca acgaatcgcc ttgttattgt tgaatcacgt tgtccctgta gcgcaaagcg
     3001 atattcagag cccgggacag cagctcctgc cagcgagaaa tccccgcgca tcgccatcat
     3061 cggatcagtc ctccggtgat gcggctgaga aagctttgcc gcgccggcag cgtcggcgcg
     3121 aggtcgggat tgagggcctc cggccgcatc gttgcgccgg ccatgagcag gccgacagcc
     3181 gttgcatagt cgggacccgc ggacgcggca ggcaaaccgg caagcccgtg ggggcgcccg
     3241 attcgcacct gcttgttgag tatgcgctga acaagctcgc gcgcgccggc gagctggcag
     3301 gcgccacccg tcagtacggc gcggcggccc gcggcgacgt cgaagccgct ggcgcgcaag
     3361 cgattctgta cttcgccaaa gatctcttcg agccgcggct ggatgatcct cgtcagcatc
     3421 gagcgcggaa cgcgcaggtc gcaatcctgg ccgttctcgc ccatctgcgg cacggcaacg
     3481 acgtcggtgc ccgtctcgat atcgcccagg gctgcgccat agagcgtctt gatccgctcg
     3541 gctgcggaaa gcggcgaggc gagcatgcgg gcgatgtccg ctgtcacata ggcgcccccc
     3601 atcggcacca catcggcatg cacgagcgcg ccttccagaa acaccgcgat ggaggtgact
     3661 cctccgccca tgtcgatgac agtcgcgcca agctgcattt cgtcgggcgt gagcgtcgca
     3721 aggccgctgg cgtacggcgc gaagagggcg ccggcgatcg aaaggtgaca acgctcgacg
     3781 gcaagtttca gattctggag cggcgatggc cggaccgcga cggcatgcat ctgcacgccg
     3841 atgcgctggc aaaacatgcc gctcggatcg cgcacgccac gcgcttcgtc gacgacatag
     3901 gaaaccggcg cgcactggat gatctcgtgg ccgtcgagcc ggcagttcgc acggccctcg
     3961 gcaaggagat cgagcagata ctcgtcggaa acgagcgcgc cttcgagcga catggcggcg
     4021 cgcgcggtca cgctttgcgg cgcgccgcaa ttgaccgaga gcaggacgtg ctgcacccgc
     4081 aggtcggcga ggttctcggc cgccgccacg gcctcacgaa tcgattcctc cacatgctcg
     4141 aggcttgtga cggttccggc cttgatcccg cgcgattccc ggagcgatgc gccgatcacg
     4201 cgaagactgg cgggttccgc gcggccgatg aggcacacga ctttcgacga gcccacatct
     4261 agcgctgcga caaggcccgt ccggtaggct ggtgattgtg cgtcgctcgt gcgcatgcga
     4321 atcgtctcgc ccattatgcc gcgtttcccc gcatttgctg cttctgcccg ttgc
//
LOCUS       DASZMC010000459         3398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4861594,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000459 DASZMC010000000
VERSION     DASZMC010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4861594"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            568..2628
                     /gene="tkt"
                     /locus_tag="VG274_09570"
     CDS             568..2628
                     /gene="tkt"
                     /locus_tag="VG274_09570"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWE06945.1"
                     /translation="MPDQIDKLCIDTIRTLSMDAVEKAKSGHPGTPMALAPLMYTLWQ
                     RHLRYDPQNPLWPARDRFVLSAGHASMLLYSTLYLAQVREVDPDGNSTGRPAVGLDEI
                     ERFRQLGSKCPGHPEYGHTTGVELTTGPLGQGVGMSVGLAIAAKWRAEHFNRPGLELF
                     DYRIWVICSDGDMMEGVSSEAASLAGHLRLPNLVWIYDQNHISIEGSTALTFDEDVAT
                     RFAAYGWNVQHVADANDTAAIDEALSVAAAAKAQPSLIIVRSHIGYGAPNKQDTKEAH
                     GEALGADEVRAAKRAYHWPEDSSFYVPDGVKERFAAGVGSRGAKLHAEWEALQRRYRD
                     EQPDLARELDQMGARRLPDGWDAGIQNFPADEKGVATRDSAGKVLNAIAASVPWLIGG
                     AADLAPSTKTLISGAGDFEPGSFGGRNFHFGIREHAMGTVVNGLTLSGIRAYGATFLI
                     FSDYMRPAIRLSALMQIPSIFIFTHDSIGLGEDGPTHQPIEQLVGLRAIPGIVVLRPC
                     DANETAECWRVIMTLANRPTCLVLSRQKLPTLDRGKYGPARGVTHGAYILADPDGAAP
                     QAIIMASGSEVSLAVAAQESLGDAGIPVRVVSMPSWELFEAQPQRYRDSVLPPGIGAR
                     VSVEAGSTIGWERYVGRDGARIGMTSFGASAPAADVYEHFGITAAAVEEAVRAEIARA
                     RSQS"
     gene            2625..>3398
                     /locus_tag="VG274_09575"
     CDS             2625..>3398
                     /locus_tag="VG274_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HWE06946.1"
                     /translation="MNRVRQLEQFGQSVWLDFLSREFLGSAEFRQLLDEDGLKGMTSN
                     PSIFEKAISHGTEYDADIRRCVSDGCGVGTIFRHLSVRDIQIAADALRPVYDTGHGAD
                     GYISIEVSPYLAYDTAASIAEARALWREIGRPNLMVKIPGTREGIPAIRTLIGEGINI
                     NITLLFARSRYEEVVEAFLAGLETLSSHSRPDSIASVASFFVSRIDSKVDAELENVLA
                     HDEGARSSVESLQGKAAIANAKLAYQYFKQAFAGERWEKL"
BASE COUNT          643 a         1055 c         1085 g          615 t
ORIGIN      
        1 agaagggtgg cagcaaaagc tgccgccatg gccggtgttg cgcgcacgga taccccgcgg
       61 ttcgatgcag tcgggttttg tttgcccgcg ctcctgttga tagctttacc agacaggacc
      121 ggctcatcca atgaattcct cgccatttct gtggccggcg ggccacaggt ttcggcggga
      181 atcggccagt ccggacgccg attcccccga attccggaac gggagcaagc gccaccggcc
      241 gccagtcgtt cggaaggctc gccgcatcct ggtggaccaa atctgatact tcgtacccga
      301 aaattgtcat ggcccggttt aaccgggcca cccagccagc gcgcgtctgc gcgctgattg
      361 gacgctccga gtggtttcgt ccgggtggcc cgctctgcga aggcgaaaaa gacggccgcg
      421 ccgcgcaccg gaccggacgc gtcggccgga gtttgccaaa tccggccttg ccgtcatgcc
      481 cggaactatc gcaacttccg cacgttggaa ttttcggctg tccggttccg cgatatgccg
      541 tccaaggctg gattcgcacg cagcatcatg ccggatcaaa tcgacaagct ttgcatcgac
      601 acgatccgca cgctctcgat ggatgccgtg gagaaagcga agtccggcca tcccggcacg
      661 ccgatggcgc ttgctccgct gatgtacacg ttgtggcagc gccatcttcg ttacgatccg
      721 cagaatcccc tctggccggc ccgcgacagg ttcgtgcttt ccgcgggcca cgcctccatg
      781 ctcctttatt cgacgctata tcttgcgcag gtgcgcgaag tcgatccgga tggcaactcc
      841 acgggccggc cggcggtggg gctcgatgag atcgaacgct ttcgccagct gggcagcaaa
      901 tgccccggtc atcccgaata cgggcacacc acgggcgtag aactgaccac cggtccgctc
      961 ggtcagggcg tgggcatgag cgtcggattg gcgatcgcgg cgaaatggcg cgccgagcat
     1021 ttcaatcgcc ccggcctcga attgttcgac taccgaatct gggtgatctg cagcgacggc
     1081 gacatgatgg aaggcgtgtc gagcgaagcc gcatcgctcg ccggacatct gcggctgccc
     1141 aacctcgtgt ggatctacga tcagaaccac atatccatcg aaggatcgac cgcgctaacg
     1201 ttcgacgagg acgttgccac ccgctttgca gcctatggct ggaacgtgca gcacgtcgcc
     1261 gatgcaaacg atacggctgc gatcgacgag gcgttgagcg tcgcggcggc ggcgaaggct
     1321 caaccctcgc tcattatcgt gcgaagccac atcggctacg gcgctcccaa caagcaggac
     1381 accaaggagg cgcatggcga agcgctcggc gccgatgaag tgcgcgccgc aaagcgggcc
     1441 tatcactggc ccgaagacag cagcttctac gttcccgacg gtgtgaagga gcgtttcgct
     1501 gccggagtgg gcagccgcgg cgccaaattg cacgccgagt gggaggcgct gcagcggcgc
     1561 taccgcgacg agcagcccga tctggcgcgc gaactcgatc agatgggcgc gcgccgcctg
     1621 ccggacggat gggatgcggg gattcagaac tttcccgccg acgaaaaggg tgtcgccacg
     1681 cgtgacagcg ccggcaaggt gttgaatgcg atcgccgcct cagtgccgtg gctgatcgga
     1741 ggcgccgccg atctcgcgcc gtcgacgaaa acactgatca gcggtgcagg cgatttcgag
     1801 ccgggctctt tcggcggacg caatttccat tttggaattc gcgaacacgc gatgggcacg
     1861 gtggtcaatg gactcacgct ctccggcata cgcgcctatg gcgcgacatt cctcatcttc
     1921 agcgactata tgcgccccgc aatccgcctc tccgcactga tgcagattcc gagcatcttc
     1981 atcttcaccc atgattcgat cggccttgga gaagacggtc ccacgcacca gccgatcgag
     2041 cagctcgttg ggctgcgcgc cattccgggc attgtggtgc ttcggccgtg cgacgccaac
     2101 gagaccgcag aatgctggcg ggtcatcatg acgctcgcca accgcccgac gtgcctcgtt
     2161 ctctcgcgac aaaagcttcc gacgctggat cgtggaaaat atggaccagc gaggggcgtg
     2221 acccatggag cttatattct tgccgatccg gacggcgcgg cgccgcaggc gatcatcatg
     2281 gcaagcggga gcgaggtttc gttggcggtg gcggcgcagg aatccctggg cgacgcgggg
     2341 atcccggtgc gcgttgtatc gatgcccagc tgggagctgt tcgaggcgca gccccaacgc
     2401 tatcgagaca gcgttctgcc gccgggaata ggcgcgcgcg tctcagtcga ggccgggagc
     2461 acgatcggct gggagcgtta tgtggggcga gatggcgcga gaattggcat gacgagcttc
     2521 ggcgcttcgg cgcctgcggc cgacgtctat gaacatttcg gcattaccgc cgcggcggtt
     2581 gaggaagcgg tgcgcgccga gatcgcgcgc gcaaggagcc agtcatgaac cgcgtgagac
     2641 agctcgagca gttcgggcaa tccgtctggc tcgattttct gagccgcgaa tttctcggga
     2701 gcgcggaatt ccggcagctg ctggatgagg atggactcaa agggatgacg tccaacccct
     2761 ccatcttcga gaaagccatc agccacggca cggaatacga tgccgacatc aggcgctgcg
     2821 tctccgacgg ctgcggagta ggcacgatct ttcgtcattt gagcgtgcgc gatatccaga
     2881 tcgcggcaga tgcgttgcgc cccgtctacg acacgggtca cggagcggat ggctatatca
     2941 gcatcgaggt ctcgccttat cttgcctatg acacggcagc ttccatcgcc gaagcgcgcg
     3001 cgctttggcg cgaaatcggc cggcccaacc tgatggtgaa gattccgggc acacgcgaag
     3061 gcattccggc gattcgcaca ctcatcggtg aaggcatcaa tatcaacatc acgcttctgt
     3121 tcgcgcgcag ccggtacgag gaggttgtcg aagctttcct ggcaggcctc gagacgctct
     3181 ccagccacag ccggcccgac tcgattgcga gcgttgcgag cttcttcgtc agccgaatcg
     3241 attcgaaagt cgacgcggag ctggagaacg tgctcgccca tgacgagggc gcacgcagca
     3301 gcgtcgaatc gttgcagggc aaagcggcaa tcgccaacgc caagcttgcc tatcagtatt
     3361 tcaagcaggc gttcgccggc gaacggtggg agaagctg
//
LOCUS       DASZMC010000460         5474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4593384,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000460 DASZMC010000000
VERSION     DASZMC010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4593384"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VG274_09580"
     CDS             <1..619
                     /locus_tag="VG274_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06947.1"
                     /translation="MGGKYLRRWPDEANPQLTRYTPQPAEVSLVDNPCIPTATFEIVK
                     EDGTTELRKFRDASAEPVAKAGARNSSEDLARVQAMHDTAVDLGATCPPADDSGDDNI
                     DDAEDDFADKFVGFPLLDRVLTLIEKLHERVSVLESEPLPGGPVLRGTRTISKSEDVG
                     GAGANVGNDPAKAFGKHLDTLPADQRALALMKFTLANPLPHAPGR"
     gene            903..2423
                     /locus_tag="VG274_09585"
     CDS             903..2423
                     /locus_tag="VG274_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06948.1"
                     /translation="MNVTAETMALMKAALKDSSADLAKAVTTAQGLTFYDLQAPAKNL
                     YPTITKLRNMTPRVGRPAGYGTACNWKQVAGVTGSGFDAMGWIPEGQRSASMSYNTAN
                     VSAPYITLGEEDYLTFEAESAAEGFEDLNATVSMRLLQKTMRKEETGLLAGNSTLALG
                     TPPAPTLSAPANTASTLPSGTYSVIVAALTLEGWLNCKGSAAMGFTPSRTITGMDGQT
                     YALNGGNSNKSANATQAVTVGSTMLAATVPVMFGAAAYAWFVGSAGSETLQAITTINS
                     ATFSAPLATGRQAATTVSADNSNNGALAFDGYLVNAFKGGIVAPQPTGTAGAGTPLTS
                     SGRGSIVEIDNLLKAMWDSNRIGPTVIFVSSQEQQNITNKVLSNASGPLLRYDVSATP
                     GQPYAISAGGQIRYYYNPYTGGGMESGPGGGDMIPVVAHPDLSPGTIFAHCEKLPEWY
                     QSNEVPNVAEVITRRDYYRIDWPLRTRRREYGIYAEEVLAVYAPFALGAITNIANG"
     gene            2609..3439
                     /locus_tag="VG274_09590"
     CDS             2609..3439
                     /locus_tag="VG274_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06949.1"
                     /translation="MAANDLCRLADVKAWLGRADTNSDAVLTALIARASRQILSWLQR
                     SVILPHTVTEIRGGNGGRFLMLRQWPVTSVTSLVVAGQTIPPMRSGGSMPGPGWALEP
                     WDGTPPGRAQEIVLCDYYFGYWGTGKTQNTQVVYQAGYQVSAEPQTIVNGTAIVTAPY
                     GAWASDVGVTYADGTPLVPVTGAPAIGQYQLTPGMPGSYTFNTGDNGAAVLISYGFVP
                     ADLADACIELASERFRYAQRIGEKTHSLGGNETVSFDNARFTPLVAALLQPYRNLLPM
                     "
     gene            3456..3935
                     /locus_tag="VG274_09595"
     CDS             3456..3935
                     /locus_tag="VG274_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06950.1"
                     /translation="MNITTEIDAARAVAKLAALPGAVRSSVRETMQAELLELLATVQA
                     KLSGGVLNARTGNLRASIEGELGEDDTSVGARVFGDGSVPYARIQEYGGRIEVPAMTP
                     VNAKALAFAYGGRMVFAKRTAAHTVTIPERSYLRSSLAEFAERFVDGIRKVALEAAR"
     gene            3932..4378
                     /locus_tag="VG274_09600"
     CDS             3932..4378
                     /locus_tag="VG274_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06951.1"
                     /translation="MSEPQFAREAIYAALFALVAGNSAFRTTTRRIKDYSAVDQATQP
                     ALLQVELGEKWNARVGLPPVVTLSARLFLYCEENDPTEPVSTQMNALLDAVMAALAPP
                     VPPHGPFRQTLGGLVQHAGISGEIVIAEGLSGQSEAVIAIEILVNP"
     gene            4548..5291
                     /locus_tag="VG274_09605"
     CDS             4548..5291
                     /locus_tag="VG274_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06952.1"
                     /translation="MQLNFGVGTAIGKRTDIANAKPSFLGVLQDLEIDIAVTLKELTG
                     QYRMPVDVAPANMKVTGKAKFARIQGASVNNLLLGQTETDSSGIDMAVAEAFGVPAST
                     PFTYTTVNSARFIEDLGVFYAGGAQLQPVASAPAQGQYSVSAGVYTFAAADEGAAMVA
                     YYSYSSTSLVQLSLANQLMGTGPVFELQAKQNYFVQGVEKKLVLKLNSCRGSKWSLPF
                     KNTDYTIQDFEFTAFCDANNNWGTFAFSE"
BASE COUNT         1032 a         1854 c         1688 g          900 t
ORIGIN      
        1 catgggcggg aaatatctgc gccgctggcc ggacgaggcg aatccgcaac tgacgcgcta
       61 caccccgcag cccgcggaag tcagcctggt cgacaatccc tgcatcccca ctgcgacctt
      121 cgagatcgtc aaggaagacg gcacgacgga actgcgcaag ttcagggacg cgagcgccga
      181 acccgtcgcg aaggccgggg cgcggaattc gagcgaagat ctggcgcgcg tgcaggcgat
      241 gcacgacacc gcggtcgatc tcggcgccac ctgcccgccc gctgacgatt cgggcgacga
      301 caatatcgac gacgcggagg acgacttcgc cgataagttc gtcggctttc cgttgctcga
      361 tcgcgtcctg acactgatcg aaaaactgca cgagcgcgtc tcagtcctgg aatccgaacc
      421 gctgcccggc ggtccggttc tgcgcggcac gcgcaccatc tccaaaagcg aagacgtcgg
      481 cggcgcgggc gcgaacgtgg ggaacgatcc cgccaaggca ttcggcaaac atctcgacac
      541 gcttcccgcc gaccagcgcg cgctcgcgct gatgaagttc accctcgcca accccctccc
      601 ccacgccccg ggccgctgac cgcgatcgcg cgcttcccaa aaaattgtca tggcccgcgc
      661 gtgaagcatg tcctccggcc gcgcttcgcg cggaccgggg ggcgggccag ccagttggaa
      721 gcgctcgtgc ggaaatcgaa caggaagaat tcccgcgcga agcgaccaac tcatcacaga
      781 tttcacctgg gtggcccgct cggagtttac actccggccg cgctctcgcg cggaccgggg
      841 tggcgggcca tgacaccgat agtttttggc aatcacgatc accaacgaaa ggaaacatgc
      901 acatgaacgt tactgctgaa accatggcgc tgatgaaggc ggcgctgaag gattcctccg
      961 ccgatctcgc caaagccgtc accaccgccc agggcctgac cttctacgac ctgcaggcgc
     1021 cggcgaagaa tctctatccc accatcacca agctccggaa catgacgccg cgcgtcggcc
     1081 ggcctgcggg ctatggcacc gcgtgcaact ggaagcaggt ggcgggcgtc acgggctccg
     1141 gcttcgacgc gatgggctgg attcccgaag gccagcgctc ggcgtcgatg agctacaaca
     1201 ccgcgaacgt cagcgcgccc tatatcacgc tcggcgagga agactacctg accttcgaag
     1261 cggaatcggc ggccgaaggc ttcgaagatc tgaacgccac cgtctcgatg cgcctgctgc
     1321 agaaaacgat gcgcaaggag gagacgggcc tgctcgccgg caattcaact ctcgcgctcg
     1381 gcacgccgcc cgcgcccacg ctctccgcgc ccgccaatac cgcgtcgacc ctgccctctg
     1441 gaacctattc cgtcatcgtt gccgcgctca cgctggaggg ctggctcaac tgcaagggca
     1501 gcgccgcaat gggcttcacg ccgtcacgca ccatcaccgg catggacgga cagacctacg
     1561 cgctgaacgg cggcaattcg aacaaatccg ccaacgccac gcaggcggtg acagtcggct
     1621 cgaccatgct cgcggcgacc gtgcccgtga tgttcggcgc cgccgcctat gcgtggttcg
     1681 tcggcagcgc gggttcggag acgttgcagg cgatcaccac catcaacagc gcgaccttct
     1741 ccgcgccgct cgccaccggc cgccaggcag cgacgaccgt ctccgcggac aattccaaca
     1801 atggcgcgct cgcctttgac ggctatctcg tcaatgcctt caagggcggc atcgtggcac
     1861 cgcaaccgac cggcacggcc ggcgccggca cgccgctcac ctcgtccggc cgcggctcga
     1921 tcgtcgagat cgacaatctg ctgaaggcga tgtgggacag caaccgcatc gggcccaccg
     1981 tgatcttcgt cagctcccag gaacagcaga acatcaccaa caaggtactg tcgaatgcca
     2041 gcgggccgct gttgcgctac gatgtcagcg cgacgcccgg ccagccctat gcgatctcgg
     2101 ccggcggtca gatccgctac tactacaatc cctataccgg cggcggcatg gagagcggtc
     2161 cgggtggcgg ggacatgatt cccgtcgtcg cccatcccga tctctcgccc ggcacgatct
     2221 tcgcgcattg cgagaagctg ccggaatggt accagtcgaa cgaggtgccc aatgtcgccg
     2281 aggtgatcac gcggcgcgac tattaccgca tcgactggcc gctccggacg cggcggcgcg
     2341 aatacggcat ctacgccgag gaagtgctcg cggtctacgc cccctttgcg ctgggcgcga
     2401 tcacaaacat cgccaacggg taagacacga agtgcatgaa ggcggctttt aacgcagggg
     2461 gcgccgggga tgcgcagggg acgcagagaa attgttgcgc gccgcgggcg cgcgatcttg
     2521 tgccgcgtcc tccgcgcatc cgccgcgccc tctgcgttga atcttccttc gtctccttct
     2581 ttgtcgtgaa ttttgcaggt tcgatccgat ggctgcgaac gatctctgcc ggctcgccga
     2641 tgtgaaggcg tggcttgggc gcgccgacac aaactccgac gcggtgctca ccgcgctgat
     2701 cgcgcgcgcg agccggcaga tcctttcctg gcttcagcgc agcgtgatcc tgccgcacac
     2761 cgtcacggag attcgcggcg gcaatggcgg ccgttttctg atgcttcgcc aatggcccgt
     2821 aacgtcggtg acctctctcg tcgtagcggg gcaaaccatt ccgccgatgc gatcgggcgg
     2881 atcgatgccc ggccccggct gggccctcga accctgggac ggaacgccgc ccggccgggc
     2941 gcaggagatt gtgctgtgcg actattactt cggctactgg ggcacgggaa aaacacagaa
     3001 cacccaggtg gtctatcagg ccggctatca ggtcagcgcc gagccgcaga ccatcgtcaa
     3061 tggaaccgca atcgtgaccg cgccctatgg agcgtgggcc agcgatgtcg gcgtcaccta
     3121 tgcggacgga acgccgcttg tgccggtgac cggcgccccc gcgatcggcc aataccagct
     3181 gacgcccggc atgccgggct cctacacctt caataccggc gacaacggcg ccgcggtgct
     3241 gatcagctac ggcttcgttc ccgccgatct tgccgacgcc tgcatcgagc tggcgagcga
     3301 acgcttccgc tatgcccagc gcatcggcga gaagacgcac agcctcggcg gcaacgagac
     3361 ggtgagcttc gacaatgcgc gcttcacacc cctcgtcgcc gccctcctcc agccctaccg
     3421 caacctgctg ccgatgtgaa ttcccggaaa aaacgatgaa cataacgacc gaaatagatg
     3481 ccgcgcgcgc cgtcgcgaag cttgccgcgc tgcccggcgc ggtgcgcagc agtgtgcgcg
     3541 agaccatgca ggcggaattg ctcgagctgc tggccaccgt gcaggcgaaa ttgtcgggcg
     3601 gcgtgctcaa tgcgcgcacc ggcaatttgc gcgccagcat cgagggcgaa ctcggcgaag
     3661 acgatacgtc ggttggcgcg cgcgtgttcg gcgacggttc ggtcccctat gcgcgcatcc
     3721 aggaatatgg cggacgcata gaagttccgg cgatgacgcc ggtgaacgcc aaggcgctcg
     3781 cgttcgccta tgggggcagg atggtgttcg ccaaacgcac cgccgcgcat acggtgacaa
     3841 ttccggaacg cagctatctg cgttcctcgc tcgccgagtt cgccgaacgc ttcgtcgacg
     3901 gcatccgcaa ggtcgccctg gaggcagcac gatgagcgag ccgcaattcg cgcgcgaggc
     3961 gatctacgcg gctttgttcg cgcttgtggc gggcaattcc gccttccgca ccacgacgcg
     4021 gcggatcaag gattattccg ctgtcgacca ggcgacgcag ccggcgctgt tgcaggtcga
     4081 gctgggcgag aaatggaatg cgcgcgtcgg cctgccgccg gtggtcacgc tgagcgcgcg
     4141 gctgtttctc tattgcgaag agaacgaccc gacggagccc gtctcgacgc agatgaacgc
     4201 gctgctcgat gcggtgatgg cggcgctcgc gccgcccgtc ccgccgcacg gcccgttccg
     4261 ccagacgctc ggcggcctcg tgcagcacgc cggaatttcc ggcgagatcg taatcgccga
     4321 aggactttcc ggccaatccg aagccgtgat cgccatcgaa atcctcgtca acccctgagc
     4381 cacgaagatt ccaaggaaga ttgaacgcgg aggacgccga ggattacgcg gaggacgcag
     4441 gggacgaatc gggcgccgtt ggcgcgcaat aattcttctg cgtcccactg cgcatcctct
     4501 gcgacctccg cgttcaattt tcctgtcttt tttgaaagga agcacagatg caactgaact
     4561 tcggcgtcgg gacggccatc ggcaagcgca ccgacattgc caatgccaaa ccctctttcc
     4621 tcggcgtgct gcaggatctc gagatcgaca tcgccgtcac gctcaaggag ctgaccggcc
     4681 agtaccggat gccggtcgac gtggcgccgg cgaacatgaa ggtcaccggc aaggcgaaat
     4741 tcgcgcgcat ccagggcgcg agcgtgaaca atctgctgct cgggcagacg gagaccgaca
     4801 gttccggcat cgacatggcg gtcgccgagg cgttcggcgt gcccgcctcc acgcccttca
     4861 cctacacgac cgtgaacagc gcgcggttca tcgaggatct cggcgtcttc tacgcgggcg
     4921 gcgcgcagct tcagcccgtc gcctccgcgc ccgcgcaggg gcaatacagc gtcagcgccg
     4981 gcgtctacac cttcgccgcg gccgacgaag gcgcggccat ggtcgcctat tacagctact
     5041 cctcgaccag cctcgtgcag ctcagcctcg ccaaccagct catgggcacg ggcccggtgt
     5101 tcgagctgca ggcgaagcag aattatttcg tgcagggcgt ggagaagaag ctggtcctca
     5161 agctcaattc ctgccgcggc tcgaaatggt cgctgccgtt caagaacacc gattatacca
     5221 tccaggattt cgagttcacc gccttctgcg acgccaacaa caattggggc acgtttgcgt
     5281 tcagcgaata ggaataagaa acaagattta acgcagagga cgcagaggat gcgcagtgga
     5341 cgccgacaaa aaatggttgc gcgccgacgg cgcgccattc ttgttccccc gcgtcctctg
     5401 cgcatcctct gcgcctactg cgctaaaatc ttgttcctta ttcctactcg ctgaaggcga
     5461 acgtgcccca gttg
//
LOCUS       DASZMC010000461         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4862896,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000461 DASZMC010000000
VERSION     DASZMC010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4862896"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..593)
                     /locus_tag="VG274_09610"
     CDS             complement(18..593)
                     /locus_tag="VG274_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06953.1"
                     /translation="MNSRILSVIALSAIAVSAAPRAQAAVTVLGNGVAHSCFEFAEFA
                     GNPTDGVATCTFALEQTTLSVKDRAATYINRGILLARNDQTQNALADYNRGLTMDADL
                     GEGYVDRGAIMIVLRRYDDAVADFDKGISLGANRLQIAYYDRAIADEALGNIRAAYED
                     YKKAAEIQPDFALATQQLARFRVVRKAADGS"
     gene            941..2164
                     /locus_tag="VG274_09615"
     CDS             941..2164
                     /locus_tag="VG274_09615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWE06954.1"
                     /translation="MSSRALPEGFLARSIGRIQPAQTIAANQKARDLKAQGRDVIALG
                     AGEPDFDTPDNIKEAAIAAIRRGETKYTAVEGIPELRKAISAKFKRENGLDYAPAQCF
                     AAPGGKSVIFHALLATLNPGDEVIMSAPYWVSYPDIVLFADARPVIVETSIENGFRLR
                     PEDLERAITPKTKWLIFNQPGNPSGACYTRAQLHALTDVLLRHPHVWILADDMYEHLV
                     YDGFRFETVAAAEPRLYDRTLTMNGVSKAYAMTGWRIGYCGGPEPLIKAMAKLQSQTV
                     SNPASISQWASVEALNGPQDFLDEFRRAFQERRDLVVSMLGQAHGIECTRPEGAFYVY
                     ASIRKLIGRRTPAGVPISTDEDFVRELLETEGVAAVHGAAFGLSPYFRVSYAASNASL
                     EEACRRIQRFCAGLR"
BASE COUNT          445 a          792 c          757 g          451 t
ORIGIN      
        1 gtgagcctgt cgaaccatca cgagccgtcg gctgctttgc gcaccacccg gaaacgggcg
       61 agctgctggg ttgcgagcgc aaagtcgggc tgaatttccg cagccttctt gtaatcttcg
      121 taggcggcgc ggatgtttcc cagcgcttca tcggcgatcg cccggtcgta ataggcgatc
      181 tgcaggcgat tggcccccag cgatattccc ttgtcgaaat cggcgaccgc atcgtcgtac
      241 cgccgcagca cgatcatgat ggcgccgcga tcgacatagc cttcgccgag atcggcatcc
      301 atcgtgagcc ctctgttgta gtcggcgagg gcattctgcg tctggtcgtt gcgcgccaac
      361 agaatgccgc gattgatata ggtcgcggcc cgatccttga cggacagtgt cgtctgttcg
      421 agcgcgaacg tgcaggtggc gacgccgtcg gttggattgc cggcgaactc ggcgaactcg
      481 aaacagctgt gtgccacgcc attgcccagg accgtaacgg cggcctgcgc ccggggcgcg
      541 gcggaaacgg cgatcgcgga aagcgcgatc acgctcaata tgcgggaatt catggcggcc
      601 ctcctccgta tgccgcagga attgtctcac ccgccccggt gctcgtccat gccggccact
      661 tcacgaattg cagaggttgc caggccggtt gtgcgcgagc ctgcctcgaa gcactttgta
      721 tgccacggtt tcttgcgatt ccgccgccat tggcgatcgc aaggggcgct tcaacttggc
      781 gtgagcccgc accctttatc tcgacgatcg acgggatgga ccgctgtgct cacgacattc
      841 cgcgcgaatc gcctgaaatc ggtgagcgag cgattgtgcc tcttgcctcc ggcatccggg
      901 cagcggatat gtcgcgcccg catacggagg attcggattc gtgagctcgc gcgccctgcc
      961 cgagggcttt ctggcccggt cgatcggccg aattcaacca gcacagacga ttgccgcgaa
     1021 ccagaaggcg cgcgatctca aggcccaggg ccgcgacgtg atcgcactcg gcgccggcga
     1081 gcccgatttc gacacacccg acaacatcaa ggaggcggca atcgccgcga tccgtcgggg
     1141 cgaaacgaaa tacaccgccg tcgagggaat tcccgagttg cgcaaggcga tctccgcgaa
     1201 attcaagcgc gagaacggcc tcgactatgc gcctgcacaa tgcttcgcgg cgccgggcgg
     1261 aaagagcgtc atcttccacg ccctcctggc gacgctcaac ccgggcgacg aagtgatcat
     1321 gtccgccccc tattgggtca gctatcccga tatcgtgctg tttgcggatg ccaggcccgt
     1381 catcgtcgaa accagcatcg agaacggctt tcgcctcagg cccgaagatc tcgagcgggc
     1441 gatcacgccg aaaaccaagt ggctgatctt caatcagccc ggcaatcctt ccggcgcctg
     1501 ctacacgcgc gcgcagctgc acgcgctcac cgacgttctg ctgcgccatc cccatgtctg
     1561 gatcctcgcc gacgacatgt acgagcatct cgtctacgac ggcttccgct tcgaaaccgt
     1621 cgccgcggca gagccgcgtc tctacgaccg cacgctgacg atgaacggtg tctcgaaggc
     1681 ctacgcgatg accggctggc gaatcggcta ttgcggcggc ccggagccgc tcatcaaggc
     1741 catggccaag ctccagtcgc agaccgtttc gaatcccgct tcgatctccc aatgggccag
     1801 cgtcgaagcc ttgaacggtc cgcaggattt tttggacgaa ttccgccgcg cctttcagga
     1861 acggcgcgat ctcgtcgttt cgatgctcgg ccaggcgcac ggaatcgaat gcacgaggcc
     1921 ggaaggcgct ttctacgtct atgcatcgat tcgaaagctg atcggcagac gcacgccggc
     1981 gggagttccg atttcgaccg acgaagattt cgtcagggag ctccttgaaa cggaaggagt
     2041 cgcggccgtg catggcgcgg ctttcgggct ctcgccgtat ttccgtgttt cgtacgctgc
     2101 gtcgaacgca tcattggagg aggcctgccg ccgcatccag cgcttctgcg ccggcctgcg
     2161 ctgacagagc gcgagcgcag ttcgcgtacc gttccgcagc aagcggaacg gccggccaaa
     2221 tcaccctctt gccagcgccg ttgcaaggcg cgagcattcc cttggggtgg tgacgcggcg
     2281 tcgtcttgag cgaagacgtg cgcccgtgct cgccccgatc cccaaccggg gccaggaggc
     2341 gagcatgtcc agaaaaaatg gaggaagtgg gccacgcgca gtggccctgg tgggaccgtt
     2401 cggcagcggc aagacgacgt tgctggagag cattctcgcc atcac
//
LOCUS       DASZMC010000462        10522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3492771,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000462 DASZMC010000000
VERSION     DASZMC010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3492771"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="VG274_09620"
     CDS             complement(<1..2026)
                     /locus_tag="VG274_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HWE06955.1"
                     /translation="MQFFSAERARLASLVLRRAGWLAIALAAGAVFFCLGLLFRLLMG
                     PVSLGPFNGDLHAALTEVVPGLDVRFDDAALEWTRTEGRVNLVILGTRVFDEGQHIIA
                     QAPKAEIGLAAGPLLSGHVVVNRIALVGVQLTLVHTKSGELRLGVESETGGSDVLKRI
                     RDAISRSHGGTSALKSFAVHEARLAFYDEETGAFVVAPEAELQISTLNNAASTVKNST
                     TANLTARIEISGKPARLYASIDLPERGNVVTGDISVSGLNVSALAHDGKSFAFLAPLA
                     LTADVTGSWTLVNGTDLRLADFGIGAYGYVNGFGRPVHVKSLRLVGRYDGATGRLLID
                     DAALAGEQASAHLTGSAALTFNRGGGLNSSSFALALDRIGIDLPGAMERAVTLGHAVV
                     KGAYTAADNSILVEEGLLSGGPLSATFAGRIILAANETPELNFDGRIGSIAVRDLLSY
                     WPLRVVPGARGWISEKVSSGRLGPVLVHAKIPAGAFDRPALPDEALSVTFPLVGATIR
                     YLNGLTPLTNVAGTGNLGGDTFKADIDSAAVGPLSLTRGRVTIAELHVHGTPANITAH
                     VTGQVPQFLALLDMKPLQYPTRFHINSSSAQGAGAFDASFRVPTIRGESIDSVGISVS
                     GTASAFAISLGPHTKISNGTVDFHVDNTQLRATGKVGIGGVNLGMDWLEAF"
     gene            2161..2631
                     /gene="bcp"
                     /locus_tag="VG274_09625"
     CDS             2161..2631
                     /gene="bcp"
                     /locus_tag="VG274_09625"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HWE06956.1"
                     /translation="MTKELRPGDRAPSFKLPTDSGSEISTAQLKGKPFIVYFYPRDDT
                     SGCTKEAISFSEEKRKFDSLGVEIIGISKDTAASHEKFKKKHKLGITLASDSEIKVAQ
                     AYGVWVEKSLYGRRYMGMDRATFLMDAKGVIKQIWRKVKVPGHVAAVLEAARVL"
     gene            2766..4253
                     /locus_tag="VG274_09630"
     CDS             2766..4253
                     /locus_tag="VG274_09630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWE06957.1"
                     /translation="MVEPLIERAWAWLHATFPERQIYIRSDGRVQFFTFGSTLQATCA
                     GLSLIFLGWVAFASVNVIFKDRIITSKDHRFQQMQANYENRVADLQLSYDELNNALVS
                     AEDRFKSTADQLEAKQQTVANLLGRKQVIEATVAGLGSGKAAAAAIKSLDVASAPSSS
                     ARQDVASDSLEADPALGRPSAPPRSTIGPAAPQASGAYFGSGASQLNVMPQSVEPQPR
                     TARPTRASFLDDTVLRFAEVLFHSSQARANAPLPKTPALQALAQQTDRVRQLDSTETS
                     LLVGVDQRISGRIGDVQRVLGRVGVNADDLERQASEAGVGGPLVSAQSARLDGVSDTV
                     FTAAYEGAVAHTEELNTLFTALRHVPLTTPVHGPQFEMTSGFGARVDPFTRRIALHTG
                     MDFAGPWGSTVTATAPGVVTFAGARGGYVNMVEIDHGFGFHTRYGHLAAVLVRPGTRV
                     SEGSPVGKLGSTGRSTGPHVHYEVWVADAVKDPGKFIEAGRHVFE"
     gene            4240..4803
                     /locus_tag="VG274_09635"
     CDS             4240..4803
                     /locus_tag="VG274_09635"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWE06958.1"
                     /translation="MFSNKNRDRDGTEVASSMPSSQPQPAKRAGRAGAPSIISADLTV
                     NGTLTSAGDVQIDGCVEGDVHSAGLVIGEKALIRGEIVAEDVTVRGRVNGSIRARKVL
                     LCSSCHVEGNILHEAFAVEAGAFFEGNCRHSDNPLSDEAARPRAAESKQAAAANPTSS
                     RPDPTPSIQPARQSSPEAVAMPLRSGA"
     gene            complement(4990..6057)
                     /gene="prfB"
                     /locus_tag="VG274_09640"
     CDS             complement(4990..6057)
                     /gene="prfB"
                     /locus_tag="VG274_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWE06959.1"
                     /translation="MFDWDRALRRLDELNAKAEDSSLWKDPQAAQALMRERQKLDISL
                     SGVRSLESELADALGLLDLAEADADAAMTRESEQLIGALRSRAEQLRLASMLSGEADA
                     NDTYLDIHAGAGGTESQDWAEMLERMYMRWAERKGFMLQLIEESAGEGAGIKSATLLV
                     KGDNAYGWLKTEAGVHRLVRISPFDANARRHTSFASVTVFPVIDQTIEIDIPDKDVRV
                     DTYRSSGAGGQHVNKTDSAVRFTHLPTGIVVSCQTERSQHQNRAICRDMLRSRLYEIE
                     LEKKQAETAAHVGQKTDIGWGHQIRSYVLQPYQLVKDLRTGVESRAPQDVLDGNLDPF
                     MAAALAQAIGGKPRETIADIE"
     gene            complement(6188..6523)
                     /locus_tag="VG274_09645"
     CDS             complement(6188..6523)
                     /locus_tag="VG274_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWE06960.1"
                     /translation="MTVLCVSSASASESPPSVDISRPHPQPPYPDSAQLNGEQGTVMV
                     DVFVHPNGRAAKFKVSRSSGFTDLDNAAVEGVLNWRYLPAIRDGETVSDWTTVKIVFQ
                     LPPAATPSN"
     gene            complement(6679..8532)
                     /locus_tag="VG274_09650"
     CDS             complement(6679..8532)
                     /locus_tag="VG274_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWE06961.1"
                     /translation="MDSSERELERTPSRVGAFNFARSARFLYATFQRELRANELVQIF
                     ACAFIGSGVGVAVAVLRALVQFLHEVDFGMMGGVLLSAGLNTDRARILVVPALGGLVL
                     GMLALVARRFATRDIVDPIEANALYGGRMSLADSLRLTASTVISNAAGASLGMEAGYT
                     QFGSGVFSGIASYFHLRRADHRVFVTAGSAAAIAAAFNAPLAGAFYGYELILGSYMPN
                     ALAPVAVAAVCGALSQRLIGGGQPLFAVAGGVPLDVHSYVLFGLMGVVAAGIAITAMM
                     AVTWTERSLKNSPIPDWLRPCVGGVALSAIASFYPQVLGSGHGAIEYYLHASLPFLPM
                     ALLLVAKLLASAISVGSGFRGGLFSSALFLGCLFGGVFAKCVSWLDPHFAVQYMAFLL
                     VGMGSVAAAIIGAPLTMVLLVLESTGDFPVTAGVLVSVIASATITRLMFGYSFSTWRF
                     HVRGLGLRGAHDVGWISELTVSRLMRSDPKTALTSMPLKALRELYPPGTAKRVFATAP
                     DGHYAGWVDMALVHDPQLDDAMEAGAVADVVQGGGEYLLPSENVRTALARFEDAQSET
                     LPVLSARADPRIVGYLTEAYALRRYAQELERRRSAELGEQGLFALGQSPPG"
     gene            complement(8573..>10522)
                     /locus_tag="VG274_09655"
     CDS             complement(8573..>10522)
                     /locus_tag="VG274_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWE06962.1"
                     /translation="LDATYPKQKILELYLNEIFLGENSYGVAAAALNYFDKSLDQLDI
                     SEAAFLAALPKAPSNYDPRFNKAAALDRRNWVIGQMAENGYITYDEARAAIAEPLVTQ
                     MRPLGIQAVDADYFVEEVRRILYTKYGERALYDGGLQVRSTLDARLQNFAVNALRAGL
                     VRYDRRHGWRGARSNVPVTGNWKETLAAVGNRSGIDTWRAAVILGFGPERSVEIGLDD
                     GARARIPFSELLWARKELHDALVGAVPERPQEVVKIGDVVYVEPVGTDGEFGLRQVPE
                     VNGAIVAMDPHTGRVLALSGGFSYASSQFDRAMQAQRQPGSAFKPFVYAAALDHGFTP
                     VSKVEDAPISLPQGPGLPMWTPKNFENKYLGLQTLRRGIELSRNLMTIRLAYEVGMKN
                     VVPYPIRFGVYDTLTPWLSNAIGAADTTLVRLTTGYAEFVNGGKKINATLIDRIQDRN
                     GRDIYRHDPRFCEGCNGRWQNQAEPLLPDDREQVIDPRTAYQIVSLLQGVVQHGTGIA
                     VSAVGKPLAGKTGTTNDSKDTWFVGFSPDLVCGVFVGFDNPRTLGRREQGATAAAPIF
                     RDFMKGALADQPATPFRVPPGIDLVTIDRHSGALVNSGDPGAIQEAFKSGTEPGLASA
                     DSDQPPNPNDNKPGATVDQGTGGLY"
BASE COUNT         2115 a         3302 c         3207 g         1898 t
ORIGIN      
        1 tgaacgcctc gagccaatcc attccgagat tgacaccacc gattcccacc tttcccgtcg
       61 cgcgcaactg ggtattatcg acatggaaat cgactgtccc gttcgagatt ttcgtatgcg
      121 gcccgagcga aatggcaaac gcgctggctg ttccgctgac agagatgccg accgaatcga
      181 tactctcgcc gcgaatcgtc ggcacgcgaa acgacgcatc gaacgcgccc gccccctgcg
      241 ccgaactcga attgatgtgg aatcgcgtcg gatattgcag cggtttcatg tcgagcaaag
      301 cgagaaattg cggcacctgg cccgtgacat gcgcggtgat gttggccggc gtgccatgga
      361 catgcagttc cgcaatcgtc acgcggccac gagtcagcga gagaggcccg acggcggcgg
      421 agtcgatatc ggccttgaag gtgtcgccgc ccagatttcc ggtccccgcg acattggtga
      481 gcggggtcaa tccattcaga tagcgaatcg ttgcgcccac aagtggaaag gtgacggaga
      541 gcgcttcgtc gggtagcgcg ggacggtcga acgccccggc gggaatttta gcatggacga
      601 gaaccggacc aaggcgtccc gacgacactt tttctgaaat ccacccgcgc gcgcccggca
      661 cgacgcggag cggccaatat gagagcagat cgcgaacggc gatgcttccg atcctgccgt
      721 cgaaattcag ctccggagtt tcgttggcgg caagaattat gcgtcccgca aatgtcgccg
      781 atagcggtcc tcccgagaga agtccctcct ccacgaggat gctgttgtcc gcggcggtgt
      841 aggcgccttt cacgacggca tggcccagtg taacggcccg ctccatcgcg ccgggcagat
      901 cgatcccgat ccggtcgagc gcgagcgcga agcttgagga attgagtccg ccgccccgat
      961 tgaaggtgag ggcggcgctt cccgtgagat gcgcgctcgc ctgctccccg gcaagagccg
     1021 catcgtcgat cagcaagcgg cctgtcgcgc catcgtagcg tcccacgagg cgcagcgact
     1081 tgacatgcac cggccggccg aaaccattca catagccgta agctccgatt ccgaaatcag
     1141 cgagtctgag atccgtaccg ttcaccagag tccatgagcc cgtgacgtcg gcggtcaacg
     1201 ccagcggcgc caggaacgca aagctctttc catcgtgggc aagtgcggaa acattcaatc
     1261 cgctgacgct gatatcgccg gttacgacgt ttcccctctc cggcagatcg atgctggcgt
     1321 agaggcgcgc gggttttccc gatatctcga tacgggcggt cagattcgcg gtggtggaat
     1381 ttttcacggt cgaagcggca ttgttcagcg tggaaatctg caattcggcc tcgggcgcca
     1441 cgacgaaggc gcctgtctcc tcgtcgtaga atgcgaggcg cgcttcatgg accgcgaaac
     1501 tcttgagtgc ggacgtgccg ccgtggctgc gcgagatcgc atcgcgaatt cgcttgagaa
     1561 cgtcgctgcc gcccgtctcg gattcgacgc cgagacgcaa ctcgccgctc ttcgtgtgca
     1621 cgagagtgag ttgaacaccg accagtgcaa tacggttgac aacgacatgc ccgctcagca
     1681 gaggaccggc ggcgagtccg atttcggctt tcggcgcctg cgcgatgata tgctggccct
     1741 catcgaagac gcgcgtgccc agaatgacga gattgacgcg cccctccgtg cgcgtccatt
     1801 cgagcgcggc atcgtcgaaa cgcacatcaa ggcccggtac gacctccgtg agtgcggcat
     1861 gaaggtcgcc gttgaacggt ccaagcgaca ccggtcccat cagtaggcgg aacagcagtc
     1921 ccagacagaa gaacaccgcc ccggcagcaa gcgcgattgc gagccagccg gcgcggcgca
     1981 ggacaagcga agcaagcctc gcgcgctcgg cgctgaagaa ctgcatacgt ggttccgaaa
     2041 ctttcaattc atctccgggc tgccgcccgg cccgtgcccc ccgcttgcac aggcaccaat
     2101 ctcagcttat cccccaagcc cgtcgacgcg taaactccgc aatccccttc ggacaaaccc
     2161 atgaccaagg aacttcgccc aggcgatcgg gcgccgtcgt tcaagctgcc caccgatagc
     2221 ggttccgaga tctcgacggc tcaactgaag ggcaagccat tcatcgtcta tttctatccc
     2281 agggatgaca catcggggtg cacgaaagag gccatttcct ttagcgagga aaagaggaaa
     2341 ttcgattctc tgggtgtcga gattatcgga atatccaagg ataccgctgc ttcacacgaa
     2401 aagtttaaga aaaagcacaa gctcggaatc acgcttgcgt cagattcgga gatcaaggtt
     2461 gcgcaagcct acggagtgtg ggtcgaaaag agtttgtatg gacggcgcta tatgggcatg
     2521 gacagagcga cattcctgat ggacgcgaag ggcgtcatca agcagatctg gcgcaaggtg
     2581 aaggtgccgg gccatgtcgc cgcagttctg gaggccgcac gcgtactttg accaacgccc
     2641 aaactgaacc tgcggttgtt cccaaccgga cttctaatgc caagggcttg aacgatcatc
     2701 cagaataggg tggaatgatg gcacaagggg ttggtggggg gtctacagga aaggaaagct
     2761 ggggcatggt ggaaccgctc atagagcgtg cgtgggcgtg gcttcacgcg acgtttccag
     2821 agcggcaaat ctatatccgc agcgatggcc gggtgcagtt tttcaccttc ggctcgacac
     2881 tacaggcgac atgcgcaggc ctctccctca ttttcctcgg ctgggtcgcc ttcgcttcgg
     2941 tcaatgtcat tttcaaagac cggatcatta cgtcgaagga tcatcgattt cagcagatgc
     3001 aggcgaatta cgagaaccgg gtggccgacc tccagctttc ctacgatgaa ctgaacaacg
     3061 cgctcgtctc ggccgaggac cggttcaaat cgactgccga tcaactcgag gccaagcagc
     3121 agaccgttgc aaatctcctg ggacgcaagc aggtcatcga ggcaactgtt gcgggcctcg
     3181 gctcgggcaa agccgcagcg gccgcgatca aatctctcga cgtcgcgagc gcgccgtcat
     3241 cgagcgcccg tcaggatgtg gcaagcgaca gtctggaggc cgacccggcg cttggccggc
     3301 cttctgcacc gccccgatcc acaatcgggc cggccgcgcc gcaggcaagt ggcgcctatt
     3361 tcggaagcgg cgcgtcgcag ctgaacgtca tgccacaatc ggtggaaccg cagcctcgga
     3421 ctgcgcgccc cacaagggca agctttcttg acgacaccgt gttgcgcttt gccgaagttt
     3481 tgttccactc aagtcaagcg cgcgcgaacg cgccgctgcc caagacgcca gcactccagg
     3541 ctctcgcaca acagaccgac cgggtgcggc agctcgactc gacagagacc agcctgctcg
     3601 tcggcgtgga ccaaaggatt tccgggcgca tcggcgacgt gcagagagtt ctcgggcgcg
     3661 tcggcgtgaa tgccgacgat ctggaacggc aggcctccga ggctggcgtc ggcggcccct
     3721 tggtttccgc ccaatccgcc cgcctcgacg gggtttccga tacggtgttc acggcggcgt
     3781 atgagggggc cgtggcgcac acggaggaat tgaacacgct gttcacggcg cttcgccatg
     3841 tgccgctaac aacgccggtg catggacccc agttcgaaat gaccagtggg ttcggtgcgc
     3901 gcgtggatcc gttcacccgg cgcatcgctc ttcacaccgg catggatttc gccggcccat
     3961 ggggatcgac cgtcacggcc acggcgccgg gagtcgttac ctttgccggt gcacgcgggg
     4021 gatatgtcaa tatggttgag atcgaccatg ggttcggctt tcacacgcgc tatggccatc
     4081 ttgccgcggt tctcgtcagg cccggaacga gggtaagcga gggctcaccc gtcggaaagc
     4141 ttggctcgac tggccgcagc accggacctc acgtacacta cgaagtgtgg gtcgccgacg
     4201 cagtaaagga cccgggcaaa ttcatcgagg caggacgaca tgttttcgaa taagaaccgc
     4261 gaccgcgacg gaaccgaagt tgcgtcatca atgccgtcat cacagccgca gcccgcgaaa
     4321 cgcgcaggcc gcgccggcgc cccttcgatc atcagtgccg atctcaccgt caatggaacg
     4381 ctgacatcgg ccggcgacgt tcagatcgac ggatgcgtcg agggtgatgt gcatagcgcc
     4441 ggacttgtca tcggggaaaa ggcactcatc cggggtgaaa tcgtggcgga ggacgtcacc
     4501 gtgcgtggcc gcgtcaacgg aagcatccgc gcccgcaagg tgctcctctg ctcgagctgc
     4561 catgtcgaag gaaacatcct gcatgaagct tttgccgtgg aggccggcgc gttcttcgaa
     4621 ggaaactgcc ggcattccga caatccgctc tccgatgaag ccgcgcggcc gagagccgcc
     4681 gaatcaaaac aggcggcagc cgcaaacccg acttcttcgc ggcccgatcc gacgccatcc
     4741 atccagcccg cccggcaaag ttcccccgaa gccgtcgcta tgcccctaag aagtggcgcc
     4801 tgaccggcca aatgccgtcc aatccggtcg aaaccgcgat ctactcatcg tgcgcatgaa
     4861 gtttcacagg gcgacagtga ggcggtgaaa atcgttgggc gctttgcgcg cgatacttac
     4921 tcaccgactc cctgcaccca tgtggatctt tttttcgggc ggctttgggc ttcaaggctg
     4981 cgggcgcggc tattcgatat ccgcgatggt ttcacgcggc ttgccgccga tcgcctgggc
     5041 cagtgcagcc gccatgaatg gatcgagatt tccatcgagc acgtcctgtg gtgcgcggct
     5101 ttcaacgccg gtgcgcagat ccttcaccag ctgataaggc tgaagaacgt atgaacgaat
     5161 ctggtgtccc cagccgatgt cggttttctg accgacatgc gcggcggttt cagcttgctt
     5221 tttttcgagc tcgatctcgt agagtcttga gcgcaacatg tcccggcaga ttgcccggtt
     5281 ctgatgctgc gaccgttcgg tctggcaact gacgacgatt ccggtgggca gatgggtgaa
     5341 acgcacggcg ctgtccgtct tgttcacatg ctggccgccc gcgcccgatg agcgataggt
     5401 gtcgacgcgc acgtccttgt ccgggatatc gatctcgatg gtctggtcga tgacggggaa
     5461 tacggtgacg ctggcgaagc ttgtgtgacg ccgcgcattg gcatcgaacg gtgaaatgcg
     5521 caccagccgg tgcacgccgg cctcagtctt cagccatcca taggcgttgt cgcccttgac
     5581 cagcagcgtc gcggacttga tgccggcgcc ctcgcccgcg ctctcctcga tgagctggag
     5641 catgaagcct ttgcgctccg cccagcgcat gtacatgcgc tcgagcattt cggcccagtc
     5701 ctggctctcg gttcctcctg cccccgcatg aatgtcgaga taggtgtcgt tggcgtccgc
     5761 ctcccccgac agcatcgatg cgagccgcag ctgttccgcg cgtgaacgga gcgctccgat
     5821 tagctgttcg gactcccgcg tcatcgccgc gtcggcgtcc gcttcagcca gatcaagaag
     5881 gcccagtgcg tccgcgagct ctgattccag ggatcgcacg ccggaaaggg aaatatccag
     5941 cttctgccgc tcgcgcatca gagcctgcgc ggcctgcgga tccttccaca acgaggaatc
     6001 ttcggccttt gcgttcagct cgtcgagccg cctaagcgct cgatcccagt caaagatgcc
     6061 tcctcagcag gccgattgcc tgcctgatct cttcaacacc gcgctcgatt tctggacgca
     6121 caaacaattc cctctggcca ccatcgatat ggccggggtc atcgcgcttg taaagacccg
     6181 tccagattca attggacgga gtggccgccg gcggaagctg gaacacgatc ttgacggtcg
     6241 tccagtccga cacggtttcg ccatcgcgaa ttgccggaag gtaacgccag ttcaacacgc
     6301 cttcgactgc ggcgttgtcc agatctgtga atccgctcga tcgtgacacc ttgaacttag
     6361 cggcacgtcc gttgggatga acaaacacgt cgaccatcac ggtgccctgt tcaccgttca
     6421 gttgcgccga atccgggtag ggaggctgcg gatgaggccg cgagatatcg acgctcgggg
     6481 gcgattccga agccgatgct gaagaaacac acaagaccgt catgcacgat gccactgcgc
     6541 acagcgaaga atttcgcaaa atgcgccaag attgcccgct cagcattgtc agctcgcacg
     6601 ttgagtccac ggcaaaattc taccgggaat ccgtcaatcc gacaatcacg acagcgtgtg
     6661 ggtgccggat cggtgttgtc agccaggcgg gctctgtccg agggcaaaaa gaccctgctc
     6721 gcccagctcg gcgctgcggc ggcgttcgag ctcctgggca tagcgccgta aggcgtaggc
     6781 ttccgtcagg tagcccacaa tgcgcggatc ggcgcgcgcc gaaagtaccg gcagggtttc
     6841 agactgcgcg tcctcgaagc gcgcgagcgc cgtgcgcacg ttttcactcg gaagcagata
     6901 ctcacccccg ccctgcacaa cgtcggcaac cgcgcctgcc tccatcgcgt cgtcgagctg
     6961 gggatcatgg acgagagcca tgtccaccca gccggcgtag tgaccgtctg gcgccgtagc
     7021 gaacacccgc ttggccgtgc ctggcggata gagctcgcgc agagccttca gcggcatcga
     7081 cgtcagcgcc gtcttcggat ccgagcgcat cagccgcgaa accgtcagtt cggaaatcca
     7141 ccccacatcg tgtgcgcccc tgagacccag tccgcgcaca tggaagcgcc aggtcgagaa
     7201 cgaatagccg aacatcaggc gcgtgatcgt cgccgacgcg atgacgctca ccagcacacc
     7261 cgcggtaaca ggaaagtcgc ccgtcgattc caggacgagc agcaccatgg tgaggggcgc
     7321 accaatgatg gccgcggcga ccgagcccat cccgaccaga agaaatgcca tgtactgaac
     7381 cgcgaaatgc ggatcaagcc atgacacgca tttcgcaaac acccctccga acagacagcc
     7441 gagaaagagc gccgagctga acaggccgcc gcggaatccc gatccgaccg agatcgccga
     7501 agcgagaagc ttggcgacga gaagcagtgc catcggcaag aacggcaaac tggcatggag
     7561 gtagtactcg atcgcgccgt gtccgctccc gagaacctgg ggatagaaac tcgcgatggc
     7621 ggagagcgcg actccgccca cgcatggccg cagccaatcc ggaatcggtg aattcttgag
     7681 gctgcgttcc gtccacgtca cggccatcat tgccgtgatc gcgattccgg cggcaacgac
     7741 acccatcagt ccgaacagca cgtaactgtg cacgtcgagg ggaacgccgc cggcaacggc
     7801 gaagagcggc tgcccacctc cgatcagacg ctgagagagg gcgccgcaaa cggcggcaac
     7861 tgcgacgggc gcgagagcat tgggcatgta gctgcccaga atgagctcgt agccgtaaaa
     7921 ggcgcccgcc agcggcgcgt tgaacgcagc cgcgatcgcc gcagcggagc ccgcagtcac
     7981 gaacacgcga tgatcggcgc gcctcaaatg aaaatagctg gcgatgcccg agaacactcc
     8041 ggagccgaac tgggtgtagc ccgcctccat gccgagcgac gcgcccgcgg cattggagat
     8101 gaccgtcgaa gctgtgagcc tcaggctgtc cgcgagcgac atgcgtccgc catagagcgc
     8161 gttagcctcg atcggatcga cgatgtctct cgtcgcgaaa cgccgcgcga cgagtgcgag
     8221 cattcccaga acaaggccgc cgagggccgg gacaacgagg atacgggccc ggtcggtgtt
     8281 gagcccggcg gacagcagca cgccgcccat cattccgaaa tcgacttcgt gaaggaactg
     8341 aacgagcgcg cgcagcaccg ccactgccac gccaaccccg gagccgataa aggcgcaggc
     8401 gaaaatctgc accagttcgt tggcgcgcaa ttcgcgctga aacgtggcgt agagaaagcg
     8461 tgccgaccgc gcgaaattga aagcgcccac tctggacgga gttctctcca gctcgcgctc
     8521 gctcgagtcc atttgccgtt ccgccggccg tggccctgta cgccacgcgc ggctagtaga
     8581 ggcctccagt accctggtcg acggtggctc cgggtttgtt gtcgttcgga ttcgggggct
     8641 ggtcggaatc ggcacttgcg agtcccggct cggttcctga tttgaaagct tcctggatgg
     8701 cgcccggatc tcccgaattc accagtgctc ccgaatgacg atcgatcgtc acgagatcga
     8761 ttcctggcgg aacacggaac ggcgtcgccg gctggtcggc gagcgcgccc ttcatgaaat
     8821 cgcggaagat cggagctgca gcggtcgcgc cttgctcgcg ccggccaaga gttcgcggat
     8881 tgtcgaatcc gacgaacacg ccgcaaacga gatcgggcga gaatccgacg aaccaggtgt
     8941 ccttcgaatc attggtcgtg cccgtcttgc cggcgagcgg ctttcccacg gcgctcaccg
     9001 caatgccggt gccgtgctgt acaacccctt gcaagagcga aacgatctgg taggcggtcc
     9061 tggggtcgat gacctgttcg cgatcgtcgg gaagcagagg ctccgcctgg ttctgccagc
     9121 gcccgttgca tccttcgcaa aagcgcggat cgtggcggta aatgtcgcgg ccgttgcgat
     9181 cctggatgcg gtcgatcaaa gtcgcgttga tcttcttgcc tccgttgacg aattcggcgt
     9241 agccggtcgt tagccgcacc agcgtcgtat cggccgcgcc gatcgcgttc gaaagccagg
     9301 gcgtcagcgt atcgtagaca ccaaagcgaa tcggataagg cacgacattc ttcatgccga
     9361 cctcgtaggc gagcctgatc gtcatcaggt tgcgcgacag ttcgatgccg cgacgcaagg
     9421 tctggagccc gagatatttg ttctcgaaat tcttgggtgt ccacatcggc aggcccggtc
     9481 cctggggcag cgagatgggt gcgtcctcga ctttgctgac cggcgtgaac ccatggtcga
     9541 gagctgcggc gtaaacaaag ggcttgaaag ccgaaccggg ctggcgttgc gcctgcatcg
     9601 cccgatcgaa ctggctggag gcgtagctaa agccgcccga gagcgcgagc acgcgccccg
     9661 tatgcggatc catggcgaca atcgctccgt tgacctcggg aacctgccgc agcccgaact
     9721 cgccatccgt tccgaccggc tcgacgtaga cgacatcgcc gatcttgacg acttcctgcg
     9781 gccgttccgg caccgctcct accagtgcgt cgtgcagttc cttgcgcgcc cacaagagct
     9841 cgctgaatgg aatacgggcg cgcgcgccgt cgtcgagccc tatctcgaca gatcgctccg
     9901 gcccgaatcc gagtatgaca gcggcgcgcc acgtatcgat gccggagcgg tttccaaccg
     9961 ccgcgagcgt ctctttccag ttgccggtaa cgggaacgtt gctgcgcgca ccgcgccagc
    10021 catgccggcg atcatagcgc acgaggccgg cgcgcaacgc attcacggca aagttctgaa
    10081 gccttgcgtc gagagtggag cgaacctgca ggccaccgtc atagagagcg cgttcgccat
    10141 atttcgtgta aaggatacgc cgcacttctt cgacgaaata atctgcgtct acagcctgaa
    10201 tgccgagcgg ccgcatctgg gtgacaagcg gttcggctat cgcggctctg gcctcgtcat
    10261 aggtaatgta gccgttctcc gccatttgcc cgatcaccca attccggcga tcgagggcag
    10321 cggccttgtt gaaacgcgga tcgtaattgc tgggtgcctt cggcaatgcg gccagaaagg
    10381 ccgcttccga gatatccagt tggtcgagcg acttgtcgaa ataattgagc gccgccgcag
    10441 ccacgccata agaattctca cccagaaaaa tctcgttcaa atagagctcg aggattttct
    10501 gcttcggata agtagcgtca ag
//
LOCUS       DASZMC010000463         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3218736,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000463 DASZMC010000000
VERSION     DASZMC010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218736"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VG274_09660"
     CDS             <1..924
                     /locus_tag="VG274_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06963.1"
                     /translation="KGASAPKAEAAHILRRLTGSEQFEQAEFAAFIRGLQDNGIEFLP
                     IDKFVECYDGYYQANPGRQKDDARFGHLKFDIHGDIARPVEMARILSKSKAPGLFLVM
                     HRHALNDAWHGTQKMWDALREIRDLGHEIGLHADPFHLITTHNDLYAGFQTALDELRE
                     HGFPIRTMTLHGDSRAHIKARKLQANDFFADEFRRSRWDGIAPEGQEMLADHVGRYKH
                     KKLYRQCGIEYVADVNMVHCGKLIVEQPMMYLSDNQRRLRIGHVAAKAVAEKTLEAPE
                     VFSIPPSFTADAAKVLARRPFLALFHPQWYR"
     gene            921..1721
                     /locus_tag="VG274_09665"
     CDS             921..1721
                     /locus_tag="VG274_09665"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HWE06964.1"
                     /translation="MSGVEFLRGKQPKRGAPKPLVDPVNKIILFWMHRCGSTTGQLWF
                     FNVAGWEKRMKGKGASQLSPEWYEEHAEAYEHLQEYYRDPAFTKIAVVRNPLERAVSA
                     YTVVTDTKSGSQWRAASRSIADPDDERRLSFNEFLDFLETEDLATANYHWRLQSAQDC
                     FDLALPDVHLARVETLQQDLDKLAKRMGKKPVAMRMNSAQTKVDDRLKDVDITSLVRS
                     DFLKLFGLDTRGVIRFPHYTRFLTDKTIPRLMKLYARDFEVLGYKPTI"
     gene            complement(1873..>2370)
                     /locus_tag="VG274_09670"
     CDS             complement(1873..>2370)
                     /locus_tag="VG274_09670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWE06965.1"
                     /translation="TKVWHFAHILGGTRIGEHCVISQNVMIGPKVSVGNGCKIQNNVS
                     LYEGVTLEDDVFCGPSMVFTNVLLPRAFVNRREEFLPTLIRKGASIGANATIVCGNTV
                     GSYAMVGAGAVVTRDVEDYALVLGAPAKRVGWVSRSGDRLGADLVCPRTGERYREDGG
                     KLSLI"
BASE COUNT          499 a          757 c          689 g          425 t
ORIGIN      
        1 aaaggcgcaa gcgcacccaa ggctgaagcg gcgcatatcc tgcgcaggct gaccggcagc
       61 gagcagttcg agcaggcgga gttcgcagcg ttcattcgcg ggcttcagga caacggcatc
      121 gaattcctgc cgatcgacaa attcgtcgaa tgctatgacg gctattacca ggcgaacccg
      181 ggccgccaaa aggacgacgc caggttcggc catctgaagt tcgacattca cggcgacatc
      241 gcccgcccgg tcgagatggc ccgcatcctg agcaagagca aggcccccgg cctgttcctc
      301 gtcatgcatc gtcatgcgct gaacgacgcg tggcacggca cacagaagat gtgggacgcg
      361 ctgcgcgaaa tccgcgatct cggccacgag atcggattgc acgccgaccc atttcatctc
      421 atcaccacgc acaacgatct ttatgccggt ttccagacgg cattggacga actgcgcgaa
      481 catggcttcc cgatccgtac gatgacgctg cacggcgatt cccgcgcgca tatcaaggcg
      541 cgcaaactgc aggccaacga cttcttcgcc gacgaattcc gccggtcgcg atgggacggc
      601 attgcgcccg aaggccagga aatgctcgcg gaccatgtcg gacgttacaa gcacaagaag
      661 ctctatcgcc aatgcggcat cgaatacgtc gccgacgtga acatggtcca ttgcggcaaa
      721 ctgatcgtgg agcagccgat gatgtatctc tccgacaacc agcggcgcct gcgtatcggc
      781 cacgtcgccg ccaaagccgt cgcggaaaaa acactcgaag cgccggaggt gttcagcatc
      841 ccgccttcgt tcaccgccga cgcggcaaag gttctcgccc gccgcccgtt cctcgccctc
      901 ttccatccgc aatggtatcg atgagcggcg tcgaattcct gcgcggcaag cagccgaagc
      961 gcggcgcgcc caagccgctc gtcgatccgg tgaacaagat catcctgttc tggatgcatc
     1021 gctgcggcag cacgacgggc cagctctggt tcttcaatgt cgcgggctgg gaaaagcgca
     1081 tgaaaggcaa aggcgcaagc cagctttcgc ctgagtggta cgaggagcac gccgaagcct
     1141 acgagcatct tcaggaatac tatcgcgatc ccgcgttcac caagatcgcc gtcgtgcgca
     1201 atccgctgga gcgcgcggtc agcgcctaca ccgtcgtcac cgataccaag agcggctcgc
     1261 aatggcgcgc cgcatcacgc tccatcgccg atccggacga cgaacgccgt ctgagcttca
     1321 acgaatttct cgacttcctc gaaacagaag acctcgcgac cgcgaattat cattggcgcc
     1381 tgcagagcgc gcaggattgc ttcgacctcg ctttgcctga tgtacatctg gcgcgggtcg
     1441 agacgttgca gcaggacctc gacaagttgg ccaaacgcat gggcaagaag ccggtcgcga
     1501 tgcgcatgaa ctccgcgcag accaaggtcg atgacaggct gaaggacgtc gatatcacct
     1561 cgctggtgcg ctccgatttc ctgaaattgt tcggcctcga cacccgcggc gtgatccgct
     1621 tcccgcacta cacacggttc ctcaccgaca agacgatccc gcgcctgatg aagttatacg
     1681 cacgggattt tgaggtgttg gggtataagc caacgatcta atgctccact tgtcatcccc
     1741 ggccgagcat cgcgtagcga tgtgagggga aggggaccca gatggaaata tctgatcggc
     1801 ttttaccgcc tgggtcccct tccctcgaat tgcacgcaag cgcgcaattc tcgccgggga
     1861 tgacaagcag agctaaatca acgacagctt cccaccgtct tcgcggtagc gctcgcccgt
     1921 gcgcggacag accagatctg caccgagccg atcaccgctg cggctgaccc agccgacgcg
     1981 tttcgccggt gcgccaagca cgagcgcgta atcctcgaca tcgcgtgtga cgaccgcgcc
     2041 ggcaccgacc atggcgtagg agccaacggt gttgccgcag acgatggtcg cgttggcacc
     2101 gatgctggca cctttcctga tgagtgtcgg caggaattct tcgcggcggt tcacgaaggc
     2161 gcgtggcagg agtacgttgg tgaacaccat cgacggacca cagaacacgt cgtcttccag
     2221 cgtcacgcct tcatagagtg agacgttgtt ctgaatcttg cagccgttgc cgacgctgac
     2281 cttgggcccg atcatcacgt tctggctgat gacgcaatgc tcgccgatgc gcgtgccgcc
     2341 gaggatatgg gcgaagtgcc agaccttcgt
//
LOCUS       DASZMC010000464         7048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1833796,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000464 DASZMC010000000
VERSION     DASZMC010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833796"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VG274_09675"
     CDS             complement(<1..771)
                     /locus_tag="VG274_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06966.1"
                     /translation="MNRSRLAALLLILALNGARAADVPGQHFSISPASLPKPYATTAV
                     DNSPQTIARPTAAMPKVPDGFAVSIFAGNVPHARWMAVAPNGDVFLAESDLGKIVVLR
                     PSADGMHAARIVDFAEAYDHPHGLAFAHGALYVADVKSIWRLSYADGQLKSTGRRRRI
                     ANTTPAGGVGHFTRDVVFDTKGNLFLTIGSRNNVDEEPLPNASVEKVNPDGSLSTFAS
                     GLRNPVGIAFYPGTNDLFAAVNERDGLGDNLPPDYFTYV"
     gene            complement(768..1706)
                     /locus_tag="VG274_09680"
     CDS             complement(768..1706)
                     /locus_tag="VG274_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HWE06967.1"
                     /translation="MPYRGARRSVQLVGAEISGDIAGQGVHVVDLDAYFERIGYRGTC
                     EPNLALLRSLHRLHPSAIAFESLDPLLGRPVAIDVQSLETKLVRMGRGGYCFEQNGLF
                     LHVLSTLGFATTPLAARVRWMLPPEAPQTPVSHMLLKTVLPEGIFVCDVGFGGQSPTA
                     PLRLEPGLMQETEHGIYRFCDAGTGYDLEMRLPDGWVAMYRFTEEPQTVRDYEVYNRF
                     TATHPASRFVNNLVAALVVGADRLTLLNTELTLQRRDGRRAHRLLAGPAELHEALTRD
                     FGIRIARAQIEIVWRRLPKPPDVADAGSQPSEGENR"
     gene            1759..2226
                     /gene="tsaE"
                     /locus_tag="VG274_09685"
     CDS             1759..2226
                     /gene="tsaE"
                     /locus_tag="VG274_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWE06968.1"
                     /translation="MASRFAGGLVVKGLAETEVLAARIASGLRSGDVVALEGDLGAGK
                     TTLARGILRTLGVAEAVPSPTFTLVQEYAAAGLRIAHYDLYRVESMTELDELGLDDAL
                     PEGVVLVEWPERAPERIPGAALNIRIEITGESERRMHLSGPQRWAHLLASKGS"
     gene            2226..3344
                     /locus_tag="VG274_09690"
     CDS             2226..3344
                     /locus_tag="VG274_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWE06969.1"
                     /translation="MDSERANSARAFLADAGWKGATLSPLPGDASTRRYIRVMRNGLS
                     AMLMDQPQNAEAAPCPPSATPDERRALGYNAVARLAGADCRRFIAAACFLRARGLAAP
                     EIYADDFTKGFVLLEDFGDDLYCDTLGGGGAEDDLYGAAIDAIAFLHAEPAPPQLAPE
                     MSLHAYDQTACLAETDLMTEWFVPLALGRRARVDEVEEHRALWRDALGSIRKHAPVFV
                     HRDYHAQNLFWLPGRTGVARVGLIDFQDAVAGNRSYDLISLLEDARRDVTPELALAMK
                     QRYATTMRQRGQELDGEAWAGELAVTAAQRNAKIAGIFTRLARRDGKPRYLSYLPRVW
                     GHLESDLEHPALANLRAWYDRTIPREKRHAGAEEGAAA"
     gene            3341..4063
                     /locus_tag="VG274_09695"
     CDS             3341..4063
                     /locus_tag="VG274_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWE06970.1"
                     /translation="MTRPKRAMILAAGLGTRMRPLTNNVPKPLVKVRGKTLIDHVIDR
                     LVAAKVELVIVNLHHHADLLKAHLAKRKDVEIRTSDETDRILGTGGGVFNALPLFEGE
                     AFFVHNADSIWVEGYGRALERMIGRWNPETMDALLLAASLVNAIGYEGPGDFLMDSEG
                     HLSRLPEQRLSPFAYPGVQIVHPRLFVGAKPGAFSINPLWDRAIEKGRLYGVRLDGIW
                     MHVGTPEALVQSEAYLADLLPA"
     gene            4068..7046
                     /gene="addB"
                     /locus_tag="VG274_09700"
     CDS             4068..7046
                     /gene="addB"
                     /locus_tag="VG274_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HWE06971.1"
                     /translation="MNARFFTIPPGTAFADVLARGVIESVGAERDPLALASATIYLPT
                     RRALRTLADSFARVLNGAALLPQLRALGDIDEEEILFDAASENLLLPPAIDPVRRRLL
                     LATLVQRWAQRRRDAPPGFAQAASMARSLARFLDDAETQQAPLDTLEQLAPQSLAEHW
                     MEVREFLILLRDEWPKLLAAEGAIDPTRRRNLLLAELARRYREKPPSTLVIAAGTTGS
                     IPATADLLDAIAHLPEGAVVLPGLDREIDDDSWDRLDPGHPQFGMKQLLGRVGISRND
                     VADWPHAVAQRPQRTMLLRETLRPAPTTDAWRALADQGGETLAQGLDGIHIVAASHPG
                     EEALAIALILREAVEEPSRMAALVTPDRNLARRVAAELMRWNIAIDDSAGTPLAHTPP
                     AAFLALLADAAADGFPPVALLALLKHPLAAGRDRPSEFRRRARALDRFVLRGRRPDPG
                     LVGLRDALDAARAEAMDSPIAPLLEELSAWFANVAAMLGPFAEAMGERAVALPELARL
                     HGDAAEALAATQEKKGGARLWAGDAGEAAADLIEALSRAGADLPPIEPSAYPVLFRQL
                     AGERAIRPAYGRHGRLAILGPLEARLQHYDVVVLGGLNEGTWPQSAAADPWLSRPMRQ
                     ALGLESPERAIGLAAHDFATLAAAPLVFLTRALKADGAPTIASRWLQRFEQLTRGLGL
                     APSRGAPYETYARLFATADHAAAPIPRPEPRPPVSVRPRELSVTEIEKWIRDPYAIYA
                     RHVLKLRPLEPLDAEIGPLDRGSVMHEILELFMKEAGETLPEQAEARLIAIADAAFAR
                     HRIPRATLALWRPRFARAASWFIEDERRRRSGIGRSFLETKGRLEIAGPAGTFVLKCR
                     ADRIDALRAGGAAIIDYKTGQPPTDPQVGLFAPQLPLEGAILDRGGFADIGPLETTQL
                     IYIRFAGGAVAGDRHIVNRDTRALVAETIARLVQLIAAFDRESTPYVPRVAPFRTDLA
                     GDYDHLARVREWSASGWSAE"
BASE COUNT         1209 a         2303 c         2314 g         1222 t
ORIGIN      
        1 cacataagtg aaatagtccg gcggcaggtt gtctcccagc ccgtcacgtt cgttcacggc
       61 tgcgaacaga tcgttggtgc cgggatagaa ggcgatgccg acgggattgc gcaaaccgct
      121 cgcgaaggtc gagagcgaac cgtcgggatt taccttctcc acgctcgcat tgggcagcgg
      181 ctcctcgtcc acattgttgc ggctgccgat ggtgaggaac agattgccct tcgtgtcgaa
      241 tacgacatcg cgcgtgaaat gccccacgcc gcccgcggga gtcgtgttcg ctatacgtcg
      301 ccgccgtccc gtgcttttca gttgaccatc tgcataggaa agccgccaga tcgacttcac
      361 atcggccacg tagagcgcgc cgtgcgcgaa ggcgagaccg tgcgggtggt cgtatgcttc
      421 ggcgaaatcg acgatccgcg cggcatgcat tccgtccgcc gaaggccgca gcaccacgat
      481 cttgcccaga tcggactcgg caaggaagac atcgccattg ggcgcaaccg ccatccagcg
      541 ggcatgcggc acgttgcccg cgaaaatgga taccgcgaac ccgtccggca ccttcggcat
      601 tgccgctgtt ggccgcgcaa tcgtctgcgg cgaattgtcg accgcggtcg tggcataggg
      661 cttgggaaga ctggcgggcg aaatggagaa atgctggccg ggcacatcgg ccgcgcgcgc
      721 gccgttcagg gcgaggatca gcagcaacgc agcaagcctg cttcgattca tcgattctct
      781 ccttccgatg gttgcgatcc ggcatccgcc acatccgggg gcttgggcag ccgtcgccag
      841 acgatttcga tctgggcccg cgcgatgcgg attccaaaat cgcgagtcaa tgcctcgtgc
      901 agttccgcgg ggccggcaag caggcggtgt gcgcgccggc cgtcgcggcg ctgaagcgtg
      961 agttcggtgt tgagcagagt gaggcgatcc gcccccacaa ccagcgccgc aaccagattg
     1021 ttgacgaagc gcgaggcggg atgagtcgcg gtgaaccggt tgtacacttc atagtcgcgc
     1081 accgtctggg gctcctcggt gaatcgatac attgccaccc agccatcggg cagccgcatc
     1141 tcgagatcgt agccggtccc ggcatcacaa aaccggtaga tgccgtgctc ggtctcctgc
     1201 atgagcccgg gttcaagacg gagcggcgcg gtcgggctct gcccgccgaa gcccacatcg
     1261 cacacaaaga ttccttccgg gaggacggtc ttgaggagca tgtggctcac gggcgtctgc
     1321 ggcgcctcgg gcggcagcat ccagcgcacc cgtgcggcga gcggggtggt cgcgaaaccg
     1381 agcgtggaca acacatgaag aaaaagcccg ttctgctcga agcaatagcc gccgcgaccc
     1441 atgcggacga gcttggtttc gagcgattgc acgtcgatcg ccaccggacg gcccagcagc
     1501 gggtcgaggc tttcgaatgc tatggcggac ggatggagcc ggtgcaggga cctgagaagc
     1561 gcgaggttcg gctcgcacgt tccgcgatag ccgattcgtt cgaaataggc gtcgaggtcg
     1621 accacatgca ctccctggcc ggcaatgtcg ccggaaattt ctgccccgac aagttgtacc
     1681 gaccggcgcg caccacggta tggcatcgcg tgttgtagcg gcgcgcccga ccggctagaa
     1741 cgcgcgcgaa aggcggacat ggcgtcgaga tttgcgggcg gtctcgtggt gaagggccta
     1801 gccgaaaccg aagttttggc tgcacggatc gcatccggcc tccggtcggg cgatgtggtc
     1861 gcgcttgaag gcgatctcgg cgcgggcaag acgacgctgg cgcgcggaat attgcgcacg
     1921 ctcggcgtcg cggaagccgt gccgagcccg acgttcacgc tggtccagga atatgcggcc
     1981 gccggtctca ggattgctca ttacgatctt taccgcgtgg aaagcatgac ggagttggat
     2041 gagctgggac tcgacgacgc acttccggaa ggcgtggtgc tcgtggaatg gcccgaacgc
     2101 gcaccggagc gcattcccgg cgccgcattg aacattcgga tcgaaatcac gggcgaaagc
     2161 gagcggcgta tgcatctgtc tggaccgcaa cgctgggcgc atctgctcgc gagcaagggg
     2221 agctaatgga cagcgagcgg gcgaattctg cgcgcgcgtt tcttgcggat gcgggctgga
     2281 agggcgccac gctttctcct ctgcccggcg acgcttcgac gcgccggtac attcgcgtga
     2341 tgcgcaatgg cctcagcgcc atgctcatgg accagccgca gaatgccgag gcggcgccct
     2401 gtccgccttc ggccacgccc gacgagcggc gggcgcttgg ctacaacgcg gtggcgcggc
     2461 tggcaggcgc cgattgccgc cgcttcatcg cggcagcctg ttttctgcgc gcgcgcgggc
     2521 tggctgcgcc ggaaatctat gcggacgact tcacgaaagg attcgtcctg ctagaggatt
     2581 tcggcgacga tctctattgc gacacgctcg ggggcggcgg tgcggaagac gatttgtatg
     2641 gcgccgcaat cgacgcgatc gcgttcctgc atgccgaacc ggcgcccccg cagctcgcac
     2701 ctgaaatgtc gcttcacgct tacgaccaga cggcctgtct tgccgaaacg gatctgatga
     2761 cggagtggtt cgtgccgctg gcgctcgggc gccgtgcgcg agtggacgaa gttgaagagc
     2821 atcgcgcgct atggcgcgac gctctggggt cgatccgcaa acacgcgcct gtcttcgtgc
     2881 atcgcgacta tcacgcccag aatcttttct ggctgcccgg gcgaacgggc gtcgcccgcg
     2941 tgggcctgat cgatttccag gatgcggtgg ccggcaaccg aagctacgac ctgatctcgc
     3001 tcctcgaaga tgcacggcgc gatgtcacgc cggaactggc gctcgcgatg aagcagcgct
     3061 atgccacgac catgcggcag cggggacaag aactcgacgg ggaagcgtgg gctggcgaac
     3121 tggcggtcac ggcggcccag cgcaatgcca agattgcagg catcttcacg cggcttgcaa
     3181 gacgcgatgg caagccgcgc tatctttcct atcttccccg cgtttggggg catctcgagt
     3241 ccgacctcga acatcctgcg ctcgcaaacc tgcgtgcgtg gtacgaccgc acaattccgc
     3301 gggagaagcg ccatgcaggc gcggaagaag gggcggcggc gtgactcgtc cgaagcgcgc
     3361 gatgatcctg gcagcagggc tcggcacccg catgcgccct ctcaccaaca atgtgccgaa
     3421 gccgctcgtg aaggtgcgcg gcaagacgct catcgaccac gtgatcgatc gtcttgtggc
     3481 agcgaaggtc gaattggtga tcgtcaacct gcaccatcac gccgatctgc tgaaggccca
     3541 tctcgcgaag cgcaaagacg tcgaaattcg cacgtcggac gagactgacc ggattctcgg
     3601 caccggcggc ggcgtgttca acgccttgcc gttgttcgaa ggcgaagcct tcttcgttca
     3661 caatgccgat tcgatctggg tcgaaggcta tggccgcgcg cttgaaagga tgatcgggcg
     3721 atggaatccg gagacgatgg acgcgcttct cctcgcggcg tcgctcgtca acgccatcgg
     3781 ctacgagggg ccgggcgatt tcctcatgga ttcggaaggt catctctccc gcttgccgga
     3841 acagcgcctc tcgcctttcg cctatccggg tgtgcagatc gtgcatccac gtctcttcgt
     3901 cggcgcaaag cccggtgcgt tctcgatcaa tccgctctgg gaccgcgcaa tcgaaaaggg
     3961 tcgcctctat ggcgtgcggc tcgacggcat ctggatgcat gttggaacgc cggaagcgct
     4021 tgttcaatcg gaagcctatc tcgcggacct cctgcccgcg tgaagcgatg aacgcccggt
     4081 ttttcacgat accgccgggc acagcctttg ccgacgtgct tgcgcgcggg gtgatcgaaa
     4141 gtgtgggagc cgagcgcgat cctctcgccc tcgcgtctgc gaccatttac ttgccgacgc
     4201 gccgcgcgct tcgcacgctc gcagattctt tcgcgcgcgt gctgaacggg gcagcgctgc
     4261 tgcctcagct tcgcgcgctc ggcgacatcg acgaggaaga gattctcttc gacgccgcga
     4321 gcgagaatct cttgctgccg cccgcaatcg atccggtccg ccgccgcctg ctcctcgcca
     4381 cgctggttca aagatgggca cagaggcggc gtgacgctcc gcccggcttc gcgcaggcgg
     4441 cttcaatggc gcgcagcctg gcacgatttc tcgacgacgc cgaaacccag caggccccgc
     4501 tcgacacgct ggagcaactc gctccccaat cgctcgccga acactggatg gaggttcgcg
     4561 aattcctcat cctgttgcgc gatgaatggc cgaagctgct cgcggcagaa ggcgcgatcg
     4621 accccacgcg ccggcgaaat cttctgcttg cggagctcgc gcggcggtac cgggagaagc
     4681 cgccatcgac gctcgtgatc gcagcgggaa ccaccggaag cattcccgcg acggccgatc
     4741 ttctcgatgc catcgcccac ctgccggaag gcgcggtcgt gctgccgggt ctcgaccgag
     4801 aaatagacga cgacagctgg gaccgtctcg atccgggtca cccgcaattt ggcatgaagc
     4861 agctcctcgg gcgtgtcgga atttcacgca acgacgttgc agactggccc cacgcagttg
     4921 cgcagcgtcc gcaacgtaca atgctgctgc gtgaaacctt gcggcctgcc ccgacaaccg
     4981 acgcgtggcg cgcgctcgcg gatcaaggcg gcgaaacgtt ggcgcagggc ctcgatggca
     5041 tccacatcgt tgccgcttcg catccgggcg aagaggcgct cgcgatcgcg ctgatcctgc
     5101 gcgaagcggt cgaggaacca tcgcgaatgg ccgcgctggt cacgcccgac cgcaatcttg
     5161 cgcgccgcgt tgccgcggaa ttgatgcgct ggaacattgc gatcgacgat tccgcgggca
     5221 cgccgctggc gcacacgcca cccgccgctt ttctcgctct gctggccgac gcggcggcag
     5281 atgggtttcc gcccgtcgca ctgctcgccc tcctcaaaca tccgctcgcc gcgggcaggg
     5341 atcgcccgag tgaattccgg cgccgcgcgc gcgccctcga ccgtttcgtt ctgcgcggcc
     5401 ggcgccccga tccgggcctg gtgggattgc gcgatgcact tgatgccgca cgcgccgagg
     5461 cgatggactc gccgatcgcg ccgcttcttg aggaactgtc tgcatggttc gcgaacgtgg
     5521 ctgcaatgct cggtccgttc gcggaagcga tgggcgagcg cgctgtcgct ctgcccgagc
     5581 tcgcgcggct gcacggcgac gctgcggaag cgcttgcggc aacacaggaa aaaaagggcg
     5641 gcgcgcggtt gtgggccgga gatgcaggag aggccgcggc cgatttgatc gaagcgctct
     5701 cgcgcgccgg cgcggatctg ccgccgatcg agccctcagc ctatccggtc ctgttccggc
     5761 agcttgccgg cgaacgcgcc atccgtccgg cttatggccg gcacgggcgt ctggcgatcc
     5821 tcggcccgct cgaagcgcgg ctgcaacact acgatgtcgt cgtgctcggc ggattgaacg
     5881 agggcacgtg gccgcaatcc gcggccgccg atccttggct gtcgcggcca atgcgccagg
     5941 ctttgggcct ggaatcgcct gaacgggcga tcggactggc cgcgcacgac tttgcgacgc
     6001 tcgccgccgc gcccctcgtg ttcctcacgc gcgcgctgaa ggcggacggc gcgccgacga
     6061 ttgcctcacg ctggcttcag cgcttcgagc aactcaccag aggcctcgga ctcgcgccgt
     6121 cgcgcggcgc gccctacgaa acctatgcgc gtctcttcgc gaccgccgac catgccgcgg
     6181 cgccaattcc gcggccggaa ccgcggccac ccgtatcggt gcgtccgcgc gagctatccg
     6241 tcaccgagat cgagaaatgg atccgcgatc cctacgcaat ctacgcgcgg catgttctca
     6301 agcttaggcc gctcgaaccg ctcgatgcag agatcggtcc actggaccgc ggcagcgtga
     6361 tgcacgagat cctcgagctg ttcatgaagg aggccggcga gaccttaccg gaacaggcgg
     6421 aagcccgatt gatcgcgatc gcggatgccg cattcgccag gcatcgcatc ccgcgagcga
     6481 cgctcgcgct ttggcggccg cgctttgccc gcgccgcgtc gtggttcatc gaagacgagc
     6541 gcaggcggcg ctccgggatt ggccgctcgt tcctcgagac caagggccgg ctggaaatcg
     6601 cgggaccagc cgggaccttt gttctgaaat gccgcgccga tcgcatcgat gcgttgcgcg
     6661 ccggaggcgc ggcgatcatc gactacaaga ccggacagcc gcccaccgat ccgcaggtcg
     6721 ggctgttcgc gccgcaattg cccctcgaag gcgccatcct cgatcgggga ggcttcgccg
     6781 atatcggtcc gctcgagacc acacagctca tctatatccg cttcgcgggc ggcgcggttg
     6841 cgggcgacag gcacatcgtg aaccgcgata cgcgggcgct ggtcgccgaa accattgcgc
     6901 gactcgtgca attgatcgcc gctttcgacc gggaatcgac tccctacgtt ccgcgcgtcg
     6961 ccccgttccg caccgatctg gcaggcgatt acgaccatct cgcccgtgtg cgcgaatggt
     7021 cggcttccgg atggagcgca gaatgagt
//
LOCUS       DASZMC010000465         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2669209,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000465 DASZMC010000000
VERSION     DASZMC010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669209"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VG274_09705"
     CDS             <1..470
                     /locus_tag="VG274_09705"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HWE06972.1"
                     /translation="DNGELQAMFAEVNNLPPDDPLRGGDTQQTAAVSSDGDAHGVSTS
                     AAVAPPPAPTETASNTDNAGLHDVAQPASPAADTQETLPAARPESGTLVTDTKHRVAV
                     PSFVGASVRQVVERAGTAGLAVQLLGNGLARAQAPAAGTLVPMGTEIVVRFAR"
     gene            651..>2008
                     /locus_tag="VG274_09710"
     CDS             651..>2008
                     /locus_tag="VG274_09710"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWE06973.1"
                     /translation="MSEALRGVEILRRIGDDEPVTGVEYDSRRVQPGSLFVAMRGETT
                     DGNRYIEQAVARGAGAVVTDSAAAYDATADGPLQVALAEVPHGRRALGIIAANVFGHP
                     QQRIRLSGVTGTNGKTTTAYLLEQLLRAAGRITALIGTIEYRIAGEVCPAPHTTPESR
                     DLLALLADGVAHGVTEGVMEVSSHALEQGRVWGVPYDVAIFTNLTQDHLDYHGTMEAY
                     FAAKRKLFDGSQVRETGARPPRVAVLNAEDDTQAALVETAQTAGSDIWSYGADHGDFR
                     ARDTVMRPNGMRFTLMSPQGEAPIEVALTGRVNVLNLLAASAAAMARGLSLAQIAENA
                     PRLHAVPGRFEAVDAGQPFTVVVDYAHTDDALRNVLALARDLVSPKRDGEKDGRVLTV
                     FGCGGDRDRTKRPRMGRAAAEASELVIVTSDNPRSEDPRAIIDEILPGTAGLPATVEV
                     EP"
BASE COUNT          377 a          595 c          679 g          357 t
ORIGIN      
        1 aggacaatgg cgaactgcag gcgatgtttg ccgaggtgaa caatcttccg cccgatgatc
       61 cattgcgtgg aggcgacacg cagcaaactg ccgcggtctc gtccgatggc gacgcgcacg
      121 gcgtctccac ctcggctgcc gttgcgcccc cgcccgcgcc gaccgagacc gccagcaaca
      181 ccgataatgc cggactgcac gatgtagcgc agcctgcatc gccggccgcc gatactcaag
      241 agactttgcc cgctgcccgg ccggagagcg gcacgctggt tacagacacg aaacatcgag
      301 tggcggtgcc atcctttgta ggcgcatcgg tgcgccaggt ggtggagcgc gctggaacgg
      361 cggggcttgc ggtgcaattg ttgggcaatg ggctggcgcg cgcccaggct cctgcggcag
      421 gcacactggt accgatgggg acggagatcg tcgtgcgctt cgcacgttag cgcaaaggtg
      481 cgcttcgcac gctaatgcaa aaattcgatt tgcgcgttag cgcaaaggtg cggttcgcac
      541 gcaaacgcaa aaatgcgctt cgcgtgttca tgcaaagctt cgatttgcgc gttagtgtag
      601 aggcgcggtt cgcacgataa gattccatca cgatgccagc ccctgtgaca ttgagtgaag
      661 cattgcgcgg cgttgaaata ctgcggcgca ttggcgatga cgagccggtc actggcgtgg
      721 agtacgactc ccgtcgcgtg cagcccggct cgctctttgt cgcgatgcgc ggagagacga
      781 cggacggcaa tcgctacatc gagcaggctg tcgcgcgtgg cgcgggtgca gtcgtgactg
      841 attcagctgc cgcctacgat gccacagccg acggaccgct gcaggtcgca cttgcggagg
      901 ttccgcatgg gcggcgtgcg cttgggatca ttgccgcaaa tgtcttcggc catccgcagc
      961 agaggattcg gctaagcggc gtcaccggca ccaacggaaa aacaacgaca gcgtatctgc
     1021 tggagcaatt gctccgggca gcaggccgca tcaccgcatt gataggaacg atcgaatatc
     1081 gcatcgcggg cgaggtgtgt cctgctccgc acaccacgcc ggagtcgcgc gatttgcttg
     1141 ccctgctggc cgacggtgtc gcgcacggcg tgacagaagg tgtgatggag gtctcctcgc
     1201 acgctctcga acagggacgt gtctggggtg tgccctacga tgtggcgatc ttcacgaatc
     1261 tgacgcagga ccacctcgat tatcacggga cgatggaggc gtactttgcg gccaaacgaa
     1321 agctcttcga cgggtcccaa gtacgcgaaa cgggagcgcg gccgccgcgt gtggccgtgc
     1381 tgaatgcgga ggacgacacc caggctgcac tggtcgagac ggcgcagacc gccggctcgg
     1441 atatctggag ctatggtgcg gaccatggcg attttcgcgc gcgcgacacc gtcatgcggc
     1501 cgaatggaat gcgcttcacc ctgatgtctc cgcaaggcga ggcgccgatc gaagtggctc
     1561 ttaccggccg ggtgaatgtg ctgaatctgc tggcggcaag cgccgcggcg atggcgcgag
     1621 gcttatcgct ggcgcagatc gctgagaacg caccgaggct gcacgctgtg ccggggcggt
     1681 tcgaggccgt ggatgcaggg cagccgttta ctgtagtggt cgactacgcg cataccgacg
     1741 atgcgttgcg caatgtgcta gcgctggcgc gtgatctggt ttccccaaag cgtgacggag
     1801 aaaaagatgg ccgcgtcctc actgtcttcg gttgcggcgg cgatcgcgat cgcaccaagc
     1861 ggccgcgcat gggtcgagcc gcggcagagg cgagtgagtt ggtcatcgtc acctcggaca
     1921 atccgcgttc ggaagacccg cgcgccatca tcgatgagat acttccgggc acagccggat
     1981 tgccggccac ggtcgaagtg gaaccgga
//
LOCUS       DASZMC010000466         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3494209,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000466 DASZMC010000000
VERSION     DASZMC010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3494209"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VG274_09715"
     CDS             <1..223
                     /locus_tag="VG274_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06974.1"
                     /translation="ISGFWKQDKGQGTISNGLFVGNSLAFSYFEPWNQARGTALLMLG
                     SDGRTLTGRWAERDGQGKETNGDWVLTRN"
     gene            complement(260..940)
                     /locus_tag="VG274_09720"
     CDS             complement(260..940)
                     /locus_tag="VG274_09720"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWE06975.1"
                     /translation="MSVETFLKQRAVNVAVNGSYSLERRWYDSAGKLRSFACRTSRVS
                     PFRMMVEVPVVGRIGERYTSYFREFGELEGTISHTMRGGFLVDMEMTAERRARFAEQL
                     AWLERKLADASVQDARVDARIIPSASHSVMILADGSVHGCFIIDISPTGAGVSAEIQL
                     PLGTPLAIGACVGRVVRVFETGFAVKFVARQDIEDLSRLIIVQPRRTTLAEERIERAL
                     GQPSAQVA"
     gene            complement(1145..1540)
                     /locus_tag="VG274_09725"
     CDS             complement(1145..1540)
                     /locus_tag="VG274_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE06976.1"
                     /translation="MAIRAGVRENRLRDPSRVTADHVRAYVDDGRTFVWIEAQKVVGF
                     SVADPRNGNIVGLFVEEGHERRGIGRALFERACDILVVAGCPRVWLTTWPGTRAERFY
                     RAAGWQVTGEDDGNLVFERPQMPPARNPP"
BASE COUNT          343 a          563 c          601 g          304 t
ORIGIN      
        1 gatttccggg ttctggaagc aggacaaggg ccaggggacg atcagcaacg ggctgttcgt
       61 cgggaacagc ctcgcgttct catattttga gccgtggaac caggcgcgcg ggactgcttt
      121 gctgatgctc ggttcggacg gccgaacgct gaccgggcgt tgggccgagc gggacggaca
      181 gggcaaggag acgaacgggg attgggtcct gacgcggaat tagcgagcag cggctcgagc
      241 gcttaatccg ggctatgcgt cacgcaacct gtgccgatgg ctggccgagc gcgcgctcga
      301 tccgctcctc ggccaaggtt gtccggcgcg gctgcacgat gatgaggcgc gagaggtcct
      361 cgatgtcctg tcgtgcgacg aacttgaccg caaatccggt ctcgaagact cggacgaccc
      421 gacccacgca ggcgccgatc gccagcggtg ttcccagcgg cagctgaatt tcggcagaca
      481 cgccggcccc tgtcggcgat atgtcgatga tgaagcagcc atgcacgctg ccgtcggcga
      541 gaatcatcac cgagtgggac gccgacggga tgatgcgcgc gtcgacccgt gcgtcctgaa
      601 cgctcgcatc cgcgagcttt ctttcgagcc aggcgagctg ctcggcgaat cgcgcgcggc
      661 gctccgccgt catctccatg tcgaccagaa agccgccgcg catggtatgg ctgatggtgc
      721 cttcgagctc gccgaactcg cggaaatacg acgtgtagcg ctcgccgatg cggccgacga
      781 ccggcacctc gaccatcatg cgaaacggcg agacgcggct ggtccggcag gcaaagctgc
      841 gcagctttcc cgcggaatca taccagcgcc gctcgagcga atagctgccg ttgacggcga
      901 cattgaccgc gcgctgcttg aggaacgttt cgaccgacac cacgacccca cacgagcgga
      961 tgctccgcgg cgatgttggt ctacagaatc taaaaacgga ttaacggtat gtgatcggtc
     1021 atgttggtgg ctgatagttg tgggaaaagg ttccagtggg tgaaatctac accgaaatcc
     1081 gcatgcgcaa tggaaccggc ggatagcgcg gattgagtaa tcgcgccttt gctcgcaacg
     1141 cgagctacgg cgggtttcgt gcgggcggca tttgcggtcg ttcgaatacg aggttgccgt
     1201 cgtcctcgcc ggtaacctgc catccggccg cgcggtagaa acgctcggcg cgcgtgcccg
     1261 gccaggtcgt cagccacacg cgaggacaac cggccacgac gagaatatcg cacgcgcgct
     1321 cgaacagggc ccgaccgatc ccgcgccgct cgtggccctc ttccacgaac agcccgacga
     1381 tgttgccgtt gcgcgggtcg gcgaccgaaa agccgacgac tttttgcgcc tcgatccaga
     1441 caaaggttct gccgtcatcg acataggcgc gaacatgatc ggcggtgacc cgggagggat
     1501 cgcgcaaccg gttctcgcgc accccggcgc gtatcgccat gatgcgcgcg atgtcgtctc
     1561 tggtcgcagg gcggatcaag cggggcctcg aagcgggatg atccgctttg cgccgcttgc
     1621 tacactcgcg gtccagtcgg gcgcaagcga gagccgcatg gacatcgaga aggagcaggt
     1681 gggagacgcc catgtggtga cggcgcatgg ccgcctcgac gggatctaca gcaccgcgtt
     1741 cgccaacgag gtcggcgcgc tgatcagcgg cacgcacccg aaaatcctga tcgacttcac
     1801 cgacatcgat c
//
LOCUS       DASZMC010000467         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4328050,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000467 DASZMC010000000
VERSION     DASZMC010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4328050"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..>1734
                     /locus_tag="VG274_09730"
     CDS             26..>1734
                     /locus_tag="VG274_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWE06977.1"
                     /translation="MRLGVELGLLALALTPVIVSGGIDLSVGSMMGLCAVVFGYLWRD
                     LHFSIAIAALCAILTGVAGGALNALVITRFGGAPLIVTLGTYSLFRGIAEGLTRGVDN
                     FSGFPAGFLYLGQGYFGGIVPAQAPVFLIFAIFYGLLLQRSIYGRAFRAIGFARRGAR
                     WAGIPVERRLALAYTLSGLTAAVASLIYVAHLGQARSDAGTGYELLAITAVVLGGADI
                     MGGRGSIWGTLLGLCAIVVLQNGLRVSAFPAELAGILTSAILVATIAAGRFGKKRIPR
                     QPAAELQEPAAELQEDEMKNSQLAILSAVIIAGSLIVAGSNWWLAKSLRASTAPAASA
                     AAAQRRLTLGVMPKAKGDPYFVSCRVGADEGAKELGADMIWDGPTDLDPAKQNEIVEA
                     WITRGVDSIAVSVVNGPGISSVLRKARARGIKVLTWDADAEPDARDFFINQATPRGLG
                     DTLGDEANRILHGSGDFAIVTGALTAANQNEWIKFIKARIAKYPGIHLIAIRPSDDDR
                     NRAFSEAQTLMKVYPNMKLIMAISAPAVPGASEAVKQSGRTDVKVTGLSLPNMCKPYI
                     QAG"
BASE COUNT          299 a          635 c          464 g          336 t
ORIGIN      
        1 ttccccctct aacgccttcg aaatcgtgcg cctcggtgtg gaattagggc tgctcgcgct
       61 cgccctcacg cccgtaatcg tgtcgggcgg catcgatctt tccgtcggct ccatgatggg
      121 actttgcgcc gtcgtgttcg gttatctctg gcgcgatctg cacttctcca tcgccatcgc
      181 cgcgctctgc gccatcctca ccggagtcgc cgggggcgct ctgaacgccc tcgtcatcac
      241 ccgcttcggc ggcgcgcccc tgatcgtcac gctcggaact tattcgcttt ttcgcggcat
      301 cgccgaaggc ctcacccgcg gagtggataa tttctccggc ttccccgccg gtttcctcta
      361 cctcggtcaa ggctatttcg gcggaattgt tcccgcccag gctccggtat ttctgatatt
      421 cgccatcttt tacggattat tgctccagcg cagcatttat ggacgcgcct ttcgcgccat
      481 cggcttcgcc cgccggggcg cccgctgggc cggcatcccc gttgagcggc gcctcgcgct
      541 cgcctacacg ctatccggtc tcactgccgc cgtcgcctcg ctgatctatg tcgcgcatct
      601 cggccaggcc aggtccgacg ccggaaccgg ctacgagctg ctcgccatca ccgccgtcgt
      661 gctcggcggc gccgacatca tgggcggacg cggcagtatc tggggaacgc tgctcggcct
      721 ctgcgccatc gtcgtactcc agaacggact ccgcgtatcc gcctttcccg ccgaactcgc
      781 cggcattctc accagcgcaa tcctggtcgc caccatcgcg gccggacgct tcggaaaaaa
      841 acgaattccc cgtcagcctg ccgctgaatt acaagagcct gccgctgaat tacaagagga
      901 tgaaatgaag aactcacaac tcgcgatact cagcgccgtt attatcgccg gatctctgat
      961 cgtcgccgga agcaactggt ggctcgccaa atcgctccgt gcatccacgg cgcccgccgc
     1021 atccgccgcc gccgcgcaac gccgcctcac gctcggcgtc atgcccaaag ccaagggcga
     1081 tccctacttc gtgagctgcc gcgtcggagc cgatgagggc gccaaagagc tcggcgccga
     1141 tatgatctgg gacggtccca ccgacctcga tcccgccaag caaaacgaga tcgtcgaagc
     1201 atggatcacc cgcggtgtcg attcgatcgc ggtctccgtt gtgaacggac ccggcatctc
     1261 ctcggtactc cgcaaagccc gcgcccgcgg cataaaagtc ctcacgtggg atgccgatgc
     1321 cgagcccgac gcccgcgatt tcttcatcaa ccaggccacc ccgcggggtc ttggcgacac
     1381 cctgggcgat gaagccaatc gcatcctcca tggcagcggc gacttcgcca tcgtcaccgg
     1441 cgctctcacc gccgccaatc agaacgagtg gattaaattc atcaaggccc gcatcgcgaa
     1501 atatcccggc attcacctca tcgccatccg gcccagcgat gacgaccgca accgcgcctt
     1561 ctccgaagcc cagaccctga tgaaggtcta tcccaacatg aagctgatca tggcgatctc
     1621 cgcgcccgcc gtacccggag cctccgaggc cgtaaagcaa tccggccgca ccgatgtcaa
     1681 ggtcaccggc ctctcgctgc ccaatatgtg caagccttat atccaggccg gtat
//
LOCUS       DASZMC010000468         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4042776,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000468 DASZMC010000000
VERSION     DASZMC010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4042776"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..733)
                     /locus_tag="VG274_09735"
     CDS             complement(17..733)
                     /locus_tag="VG274_09735"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWE06978.1"
                     /translation="MPRDLWLFAIAVSVAWIMRTEAHAGVTIPSAQTQSPDTKSPAPN
                     GPHLCRTYYPRYAVRAHEEGTTTLAFTVTAQGNVKNVSVKTSSGHASLDDASITCVGQ
                     WRYRPATKSGVPFDAPLAASIVWSLQEPTSLPPVFYTIVASVQGCIRNAHPSDEALAK
                     ATGMTVAKIQFYRSEAPTVDVTAPSGNEDLDKLVIQCLNELPPTIVGAPPIKFGFIIP
                     FSWKTDASANPSALPAATSR"
     gene            complement(878..4486)
                     /gene="metH"
                     /locus_tag="VG274_09740"
     CDS             complement(878..4486)
                     /gene="metH"
                     /locus_tag="VG274_09740"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWE06979.1"
                     /translation="MSESDFRGQRFAAHDHDLKGNNDLLTLTRPEVVRQISHDYLAAG
                     ADIIETNTFNSTSVSQADYGLSHLVRELNEAGAALAREICDQASTSTRPRLVAGVLGP
                     TNRTASLSPDVNDPGFRNISFDELRGTYRDSVLGLIAGGADLILIETVFDTLNAKAAI
                     FAAEEAFDEAGERLPIWISGTITDLSGRMLTGQTAEAFWHSVRHVRPFAVGLNCALGA
                     KELRPYVDELSQAADTLVSAHPNAGLPNAFGGYDDGPESMAAVMGEFARSGLVNIIGG
                     CCGTTPAHIKAFADAVRGVTPRVIPEKPRFLRLSGFEPFTLTPQTNFVNIGERTNITG
                     SAKFRKLIAADDYDAALEVARSQVENGAQIIDVNMDEGLIDSEAAMRRFLSLVAAEPD
                     IARVPVMIDSSKWSVIEAGLKVVQGKPIVNSISLKEGEEPFIEHARKVLRYGAAVVVM
                     AFDEQGQAETAERKFAICARAYDILVNRVGFPPEDIIFDPNIFAVATGIEEHNAYARG
                     YIEAARRIREGLPHSHVSGGLSNVSFSFRGNETVRQAMHSVFLYHAIEAGMDMGIVNA
                     GQLAVYEDIPLELREGVEDVILNRRPDATERLLDLAQRFKNDSGGAREETTLAWRQAP
                     VDDRISYALVHGIADFIIEDTEEARLKSVRPLDVIEGPLMGGMNTVGDLFGAGKMFLP
                     QVVKSARVMKKAVGHLIPFMEQESAAMREPTGRIVMATVKGDVHDIGKNIVGVVLQCN
                     GYEVIDLGVMTPAQKILDTAREKRANLIGLSGLITPSLDEMCHVAAEMERQGFDIPLL
                     IGGATTSRVHTAVKIDPNYRRGQTVHVVDASRAVGVAAQLIGTATRVPYAANVRREYA
                     EIAERHAGANRAPGRRLSLGAARANRLKFDWPRYTPPRPRFLGLREFQTYDPAELVPY
                     IDWTPFFRTWELVGPYPSILDDPAVGEAARALFADAQAMLEKIVRERWLTARAVIGFW
                     PANAETDDILIYDNESRDAPPTVFHTLRQQMSRESNRPNLALADFVAPRESGAADYLG
                     GFVVTTGHGEEAHIKRFEADKDDYSAILLRALADRLAEAFAERLHARVRREFWAYAPD
                     ENLTCEELIAEAYRGIRPAPGYPAQPDHTEKDTLFTLLDAERRAGVKLTESFAMWPAS
                     SISGLYFSHPESRYFGIGKIERDQVEDYARRKHWSVKEAERWLAPILNYDPRAAAA"
BASE COUNT          816 a         1407 c         1418 g          857 t
ORIGIN      
        1 caccgcgata tctcgctcat cttgaagttg cggcaggcaa ggcggaaggg tttgccgacg
       61 cgtccgtctt ccacgagaag ggaatgataa acccgaactt aatgggcggg gcgccgacaa
      121 tcgtcggtgg cagttcgttc agacactgta tgacaagctt atccaagtct tcgttcccgc
      181 ttggtgccgt cacatcaacg gtgggagctt cgctacgata gaactgaatc ttggcaacgg
      241 tcatgccggt agcctttgcc aatgcctcgt cgcttggatg cgcgttcctg atgcagccct
      301 gtactgacgc cactatggtg tagaagactg gtggcagcga ggtcggttcc tgtaaggacc
      361 acacgatgga tgccgccaat ggcgcgtcga aggggacacc ggacttcgtt gcaggccgat
      421 atctccattg cccgacgcat gtgatcgaag cgtcgtcaag gctagcatga ccgctggacg
      481 ttttgacgct gacgttcttc acgtttccct gtgctgtgac cgtgaaggcc agcgtcgttg
      541 ttccctcttc atgggctcgt acggcgtatc gaggatagta ggtcctgcaa agatgcggcc
      601 cgtttggggc aggagacttt gtatctggcg attgcgtttg cgctgaaggg atcgtgacac
      661 cagcatgcgc ctccgtcctc atgatccagg ccaccgagac agcgatggca aagagccaga
      721 gatcgcgcgg cattgtctcc cccgatgcgt aagaggccgc caatctagtt cttcggcacc
      781 gtggttgcta ggccggcttc tgtgcacaac agcgcaccgg acgggattcg aaccagtggt
      841 ccctcgatcg gagatttaat ggcaaatcct gccaagccta cgccgccgcg gcacgcggat
      901 cgtagttgag gatcggcgcc agccagcgtt ccgcttcctt tacgctccaa tgtttgcgcc
      961 gcgcataatc ttccacctgg tcgcgttcga tcttgccgat gccgaaatag cggctttccg
     1021 gatgggagaa atagaggccg gagatcgacg acgccggcca catggcgaag ctttcggtga
     1081 gcttcacgcc ggcgcgcctc tcggcgtcga gcagcgtgaa cagcgtgtct ttctccgtgt
     1141 ggtcgggctg ggcgggatag cccggcgccg gccggatgcc ccggtaagct tcggcgatca
     1201 gttcctcgca ggtcagattt tcgtccggcg cataggccca gaactcccgg cggacgcgcg
     1261 cgtgcaaccg ctccgcaaag gcttcggcca ggcggtcagc gagcgcgcgc agcagaatgg
     1321 cgctgtaatc gtccttgtcg gcttcgaaac gcttgatgtg cgcctcctcc ccatgtcccg
     1381 tggtgacgac gaaaccgccg agataatcag ccgcgccgct ctcgcgcggc gccacaaaat
     1441 cggcgagcgc aaggttgggc ctgttcgact cgcgcgacat ctgctggcgc aaggtgtgaa
     1501 acacggtggg tggcgcatcg cgcgattcgt tgtcgtagat caggatgtcg tcggtctccg
     1561 cgttcgcggg ccagaaaccg atcacggccc gcgcggtcag ccagcgctcg cgcacgatct
     1621 tctcgagcat cgcctgcgcg tcggcgaaga gggcgcgtgc cgcttctccg accgccgggt
     1681 cgtccagaat cgacggatac gggccgacca gttcccaagt gcggaagaac ggagtccagt
     1741 cgatataggg cacgagttcg gcgggatcgt acgtctggaa ttcgcgcaag ccgaggaaac
     1801 gcggcctggg cggcgtgtag cgcggccagt cgaatttcag acggttggcg cgcgctgccc
     1861 caagcgacag ccggcgtccg ggcgcgcggt tcgcgcccgc gtggcgctcg gcaatctccg
     1921 cgtattcgcg gcgcacattg gcggcgtagg gcacgcgcgt cgcggtgccg atgagctgcg
     1981 ccgcgacgcc cacggcgcgc gaggcgtcca cgacgtgaac cgtctggccg cgccggtaat
     2041 tgggatcgat cttcaccgcg gtgtgcacgc ggctcgtggt ggcgccgccg atcagaagcg
     2101 gaatgtcaaa accctgccgc tccatttccg cggccacgtg acacatctcg tcgagcgacg
     2161 gggtaatcag tccggaaagc ccgatcagat tggcgcgttt ctcgcgcgcg gtgtcgagaa
     2221 tcttctgcgc cggcgtcatg acgccgagat cgatcacctc gtagccgttg cactgcagaa
     2281 ccacgccgac gatgttcttg ccgatgtcgt gcacgtcgcc cttcacggtc gccatgacga
     2341 tgcggcccgt cggctcgcgc atcgcagcgc tttcctgctc catgaacggg atgagatggc
     2401 caaccgcctt cttcatgacg cgggcggact tcaccacctg cggcaggaac attttccccg
     2461 cgccgaacag atcgccgacg gtgttcatgc cgcccatcag cggaccttcg atgacgtcga
     2521 gcggccgcac cgatttcagg cgggcttcct ccgtatcctc gatgatgaaa tcggcgatgc
     2581 cgtgcacaag cgcataggag atccggtcgt ccaccggagc ctggcgccag gcgagcgtcg
     2641 tctcttcgcg ggcgcctccc gaatcgttct tgaaacgttg cgcgagatcc agcaaccgct
     2701 ccgtcgcgtc cgggcggcga ttgaggatca cgtcttccac gccttcccgc agctcgagcg
     2761 gaatgtcctc gtacaccgcg agctgtccgg cattgacgat gcccatatcc atgccggcct
     2821 caatcgcgtg atagagaaag accgaatgca tcgcctgccg gaccgtctca ttgccgcgaa
     2881 atgaaaagga gacgttggaa agtccgcccg acacatgcga atggggcaag ccttcgcgaa
     2941 tgcgccgcgc cgcctcgatg tagccgcggg cataggcatt gtgctcttcg atgccggtag
     3001 cgacagcgaa gatgttggga tcgaagatga tgtcttccgg cggaaagccg accctgttca
     3061 ccaggatgtc ataggcgcgg gcgcagatgg caaatttgcg ctccgcggtt tccgcctgac
     3121 cctgttcgtc gaaagccatc accacgaccg ccgcgccgta gcgcaggacc ttccgcgcgt
     3181 gttcgataaa cggttcctca ccctccttga ggctgatgga attgacgatg ggcttgccct
     3241 gcaccacctt cagccccgcc tctatgacgc tccatttcga cgaatcgatc atcaccggta
     3301 cgcgtgcgat gtctggttcg gcggcgacga gcgacaggaa tcgccgcatg gcggcttccg
     3361 aatcgatcag cccctcgtcc atgttcacgt cgatgatctg cgcgccgttc tcgacctggc
     3421 tgcgcgccac ttcgagagcg gcgtcgtaat cgtcggcggc aatcagcttg cggaatttcg
     3481 ccgagccggt gatgttggtc cgctcgccga tattgacgaa attcgtctgc ggcgtgagcg
     3541 tgaacggctc gaagccggag aggcgcagga agcgcggctt ttcgggaatg acacgcggcg
     3601 ttacaccgcg gaccgcatcg gcaaacgcct tgatatgcgc gggcgtcgtt ccgcagcaac
     3661 ccccgatgat attgacgaga cccgagcgcg cgaactcgcc catgacggcc gccatgcttt
     3721 ccgggccgtc gtcgtaaccg ccgaacgcgt tcggcagacc agcattggga tgggcgctga
     3781 caagcgtgtc ggcggcctgg gacaattcat cgacataggg acgaagctcc tttgctccga
     3841 gcgcgcagtt gagacccacg gcaaagggtc tgacatgccg cacggaatgc cagaacgcct
     3901 ccgcggtctg cccggtgagc attcgacccg aaagatccgt aatcgtaccc gatatccaga
     3961 tcggcaggcg ctcgcccgct tcatcgaagg cttcctcggc ggcaaagatc gcagccttcg
     4021 cattgagcgt gtcgaagacg gtctcgatca ggatgagatc ggcccctccc gcaatgagcc
     4081 cgagaacgct gtcgcggtac gtgccgcgca actcgtcgaa gctgatgttt cggaagcccg
     4141 gatcgttgac gtcgggcgac agggatgccg tccgattagt cgggcccagc acgcccgcca
     4201 ccagacgagg gcgtgtcgaa gtcgacgcct ggtcgcaaat ctcgcgggcg agcgccgcgc
     4261 cggcctcgtt caattcgcgc acgagatgcg aaagtccgta atcggcctgc gacaccgacg
     4321 tggaattgaa tgtgttggtc tcgatgatgt cggcgcccgc ggcgagatag tcgtggctga
     4381 tctgccgaac gacctcgggc cgcgtgagcg tcaggagatc gttgttgccc ttgagatcgt
     4441 ggtcatgcgc ggcgaagcgc tgcccgcgaa aatcggactc gctcaatccg tagccctg
//
LOCUS       DASZMC010000469         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5422166,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000469 DASZMC010000000
VERSION     DASZMC010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5422166"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VG274_09745"
     CDS             <1..879
                     /locus_tag="VG274_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06980.1"
                     /translation="RRDFTMNALYASADGEVFDSVGGVEDLNARRVRFVGDPVTRIRE
                     DYLRILRLFRFHAWYGEGEIDSAALHAAAAEKAGLAGLSGERVQKEVLRLLGAEDPMQ
                     SLRAMAATGILGDVLPGALGLPRLERIIAIDTDNFFEADAVLRLAALLPADGAGAAAD
                     RLKLSNAQRERLADLARVQEKIVSYLSIKEVRKLLYVLGAQRFKDRVFLKWAEDPKAS
                     NVMQWRALLALADSWQRPKFPLTGENVMAAGVTQGPLIGRILGEVEEWWIDSDFIEDE
                     FSLAERLKAVVQATAY"
     gene            881..1729
                     /locus_tag="VG274_09750"
     CDS             881..1729
                     /locus_tag="VG274_09750"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWE06981.1"
                     /translation="MEFTFLDALSLPGNPAKPNEDAWGQTSGAAVVLDGATSLGEALL
                     PGESDPAWLARFAARRLMAHCQDGAEHRDAVRNALADAEKSFHGLRKRAPKEGYENPY
                     ASMMFAGASDKGIEALWFGDCVALVKRPDEPVEIIGDAFDKRAAESRRVAMLAKAKGL
                     APAAGVNRPEFLSALRIARNTVNTEKGGWLFGPNAEAAEHVRSQHVEAQPGTLMLLAS
                     DGFLALASDYGIHDAKSLVATAETKGLDALGQQLRAVEADDPDGVKFPRFKTSDDATA
                     VLLRLT"
     gene            complement(1726..>2122)
                     /locus_tag="VG274_09755"
     CDS             complement(1726..>2122)
                     /locus_tag="VG274_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06982.1"
                     /translation="LLLWVAWRLFRDVRHMNAEPHENGEAVRCAPEINEPGGGAILMR
                     RAILQITIADISMSLDNVLAVAGVAIHHVWVLAVGLVLSIGLMGIAANMVAKMLNRHP
                     WISYAGVFIVLYVALNMIWHGGMLMVSPP"
BASE COUNT          415 a          621 c          709 g          377 t
ORIGIN      
        1 cgccgcgatt tcacgatgaa cgcgctttac gcctcagccg atggcgaagt gtttgacagc
       61 gtcggtggcg tcgaggattt gaacgcgcgc cgcgtgcgct tcgtcggcga tccagtgacg
      121 cgtatccgcg aggattatct gcgcatcctg cggctgttca ggttccatgc atggtacggc
      181 gagggcgaga tcgacagcgc ggcgcttcat gccgcggccg ctgagaaagc cggtctcgct
      241 gggctttccg gtgaacgcgt gcagaaagaa gtgttgcggc tgctcggtgc cgaagatccg
      301 atgcagagtc tgcgtgcgat ggcggcaacc ggaattcttg gcgatgtatt gccgggcgcg
      361 ttgggcttac cgcgtctgga acgtatcatc gccatcgaca cggacaattt cttcgaggcg
      421 gacgcggtgt tgcgtctggc ggcgctcctt ccggccgatg gtgccggagc ggctgcagac
      481 aggctcaaac tctcaaacgc gcagcgcgaa agactggccg atctggcgcg ggtgcaggag
      541 aagatcgtct cttatctgtc gatcaaagaa gtgcgcaaac tcctttatgt attgggcgcg
      601 cagcgcttca aggaccgtgt cttcctgaaa tgggcggaag atccgaaagc ctccaatgtg
      661 atgcagtggc gcgcgttgct ggcgcttgcg gactcgtggc aacggccgaa attcccgctc
      721 accggcgaga acgtcatggc ggcgggtgtc acgcaagggc cgctgatcgg acgcatcctc
      781 ggcgaagtcg aggaatggtg gatcgacagc gatttcatcg aagacgaatt ctcgctcgca
      841 gaacggctca aagccgtggt gcaggcgacg gcctactgag atggaattca ccttcctcga
      901 cgcgctctcg ctgcccggca atcccgccaa gccgaatgaa gacgcgtggg ggcagaccag
      961 cggcgcggcg gtcgtgctcg atggtgcgac gagccttggc gaggctttgc tgcccggcga
     1021 aagtgatcct gcatggcttg cgcggttcgc ggcgcggcgc ctgatggcgc attgccagga
     1081 cggcgcagaa catcgcgatg ccgtccgcaa cgcgctggcg gatgcggaga aatccttcca
     1141 cggccttcgc aaacgcgcac cgaaggaagg ctacgagaat ccgtatgcct cgatgatgtt
     1201 cgcgggcgca tcggacaaag gcatcgaggc gctgtggttc ggcgattgcg tcgcgctggt
     1261 aaaacgtccc gatgaacccg tagaaatcat tggcgacgcc ttcgacaagc gcgcggcgga
     1321 atcgcgccgt gtcgcaatgc tcgccaaggc caaaggactg gcgccggcag cgggcgtcaa
     1381 tcgcccggag tttctgtccg cgctgcgcat cgcccgcaac acggtcaaca ccgaaaaagg
     1441 cgggtggttg ttcggaccga acgccgaggc agcggaacat gtgcgcagcc agcatgtcga
     1501 ggcgcagccc ggcacgctca tgctgctcgc gagtgacggg tttctggcgc tggcgagtga
     1561 ctacggaatc cacgatgcca aaagtcttgt cgctacggcg gaaacgaagg ggctggacgc
     1621 gcttggccag caattgcgcg cggtggaagc ggacgatccg gacggcgtca aatttccgcg
     1681 cttcaagacc agcgacgatg cgacggcagt gctgctgcgc ctgacttagg gtggcgatac
     1741 catcagcatg ccgccgtgcc agatcatgtt cagcgcgaca tagagcacaa tgaacacgcc
     1801 ggcgtagctg atccagggat ggcgattgag catcttggcc accatgttgg ccgcgattcc
     1861 catcaatccg atggagagca cgagaccgac cgccagaacc cagacgtgat ggatcgcgac
     1921 accggcaacc gcgagcacgt tgtcgagcga catcgagata tcggcgatgg tgatctgcag
     1981 gatcgcgcgg cgcatgagga tcgcgccccc gccgggttcg ttgatttccg gtgcgcatcg
     2041 tacggcctcg ccgttctcgt gcggctcggc attcatgtgg cgcacgtcgc ggaacaaacg
     2101 ccatgcgacc caaagcaaaa ga
//
LOCUS       DASZMC010000470         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3745733,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000470 DASZMC010000000
VERSION     DASZMC010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3745733"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2327
                     /locus_tag="VG274_09760"
     CDS             <1..2327
                     /locus_tag="VG274_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HWE06983.1"
                     /translation="SAIVVLALGAGANIGAFSAVRDLVWRPLPYPSPERLVSLFETAS
                     DGSPRAVAEANLLDWAAQAHTFDSMAAYRPRSFGLTFGDTGAPTVIQTGQTMATFFQV
                     AAIRPALGRSFSSEEETAEAHVIVLTDRLWRGMFAADPQAPGRVVELNEEPYTIVGVM
                     PAGFEFPMGDVQPDAFIPLSRRDYCCGRTGSLSAAGRVKPGFSLDAARAEFAAVASNV
                     AAIHPATNRGGSAGLLPLSEAFTASRRESLLLLYAASSLLFLIACANVSGLMLARWMS
                     RQRELAIRVSLGARGRQLAAPFFAEAAILAFVAAALGLFAAEGIERAIPRFLTGVPVP
                     PDLDGYSFAFAAVLACVTTIVTASAPLLITRRASLNSTLKGGATRLRGAFAIAQIALS
                     VALLLSAGLLLHSFLNLVNADPGFTTARAWRFGIGIPERRYDTEAKEIQFHQRLLERL
                     AALPDVSSAGATGRLPLGGSPGSGGAFQIAGAGIPPVERPRARTNVASPAYFASMGIP
                     LLRGRDFSWTEDRPGGRRVVIVNQAFASGFLRDRDPIGASLELGWTSELNPRNSLWEV
                     VGVAGNTRQNSMDRNPAPEIFLSMTQTGADGAGYVIRARDGRGDIARVIAETVVQFDP
                     RVQRVHPTPLVTLIERNLDSRATAIKLIGGFGAVALLLTAAGIFGIVAFHAQERSREM
                     AIRMALGSPTADVQRLIILQALRLAGVGSLIGVFGFWWGSPWLRNQLYGVGRADAATF
                     AGVLALVAAVAVSASLAPSRRASRTDPARLLREG"
     gene            complement(2347..2751)
                     /gene="vsr"
                     /locus_tag="VG274_09765"
     CDS             complement(2347..2751)
                     /gene="vsr"
                     /locus_tag="VG274_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HWE06984.1"
                     /translation="MDTFSAAERSAVMKRVRSKDTQPEMAVRSMVHRMGFRYALHRRD
                     LPGNPDMVFPSRQRIIFVHGCFWHGHSCRSGRNRPASNTDYWIAKLNRNVARDSSNRR
                     RLRSLGWRMLVVWECELKDPERLRARIARFFE"
     gene            2881..>3483
                     /locus_tag="VG274_09770"
     CDS             2881..>3483
                     /locus_tag="VG274_09770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWE06985.1"
                     /translation="MTPNPPIEDADVHIPTRDIDIPAYQALPAAPGPFPIVLIAPEIF
                     GVNEHIRSVARRFASEGYYAIAPDLFIRQGDVSKLTDSQEIQQIIAKVPDMQVMSDLT
                     AAAEFASASGRGNIERLLMTGFCWGGRIAWLYAAENSHLRAAVAWYGRLTAPLDALHE
                     RHPLNITDDLKCPVLGLYGGQDKSIPLEHVERMRAEIARSQ"
BASE COUNT          559 a         1171 c         1019 g          734 t
ORIGIN      
        1 tttccgccat cgttgttctt gcgctcggcg cgggggccaa tattggagcc ttttccgcag
       61 tccgtgactt agtctggcgg ccgctgccct acccgtcacc ggagcgtctg gtctcgctct
      121 ttgaaactgc atctgacggc agtccccgcg ccgtcgccga agccaacctg ctcgattggg
      181 ctgctcaggc tcacaccttc gactccatgg ctgcctatcg tccgcgttcg tttggtctta
      241 ctttcggcga tacgggcgcg cccacggtaa ttcagaccgg ccaaacgatg gcgaccttct
      301 tccaggtcgc cgcaattcgg cccgctctcg ggcgctcctt cagttcagaa gaagagaccg
      361 cggaggcgca cgtcatcgtc cttaccgatc gcctctggcg cggcatgttc gcggccgacc
      421 cgcaggcgcc cggccgggtg gttgaactga acgaggagcc ctacacgatc gtcggcgtga
      481 tgcccgcggg ttttgagttc cccatgggcg acgtgcagcc cgatgccttc attccgctca
      541 gccgccgcga ttactgttgc ggccgcaccg gctcactgag cgctgccggg cgcgtgaaac
      601 ccggcttttc tctcgacgcc gcgcgcgccg aatttgcagc cgtcgcctcc aacgtcgccg
      661 ccattcaccc tgcgaccaat cgcggcggca gcgcgggcct gcttccgctc tccgaagctt
      721 tcacggcctc gcggcgtgaa agcctcctgc tgctctatgc ggcatcctcg ctgctctttc
      781 tgatcgcttg cgctaacgta tccggcttga tgctggctcg ctggatgtcg cggcaacgcg
      841 aacttgcgat acgggtctcg ctcggggcgc gcgggcgtca actggcggct ccattctttg
      901 ccgaggccgc catcctcgcg ttcgttgcgg cagcgcttgg gttgtttgcc gccgagggga
      961 tcgagcgcgc tatcccgcgc tttctgactg gagttcccgt gccaccggat ctcgacggtt
     1021 attcgtttgc ttttgccgcc gtattggcct gcgtcacgac tatagtcact gcaagcgcgc
     1081 ctttgctaat cacgcggcgc gccagtctga attcaacgtt gaagggcggc gcaacgcggt
     1141 tgcgcggcgc ctttgctatc gcgcagatcg cccttagcgt ggccctgctg ctgagcgccg
     1201 gactgctgtt gcacagtttc ctgaaccttg tgaacgccga cccaggcttc acaactgctc
     1261 gcgcctggcg ttttggaatc ggaattccgg agcgccgcta tgacaccgaa gccaaagaga
     1321 tccaatttca tcagaggctg ttggagcgct tggccgcgct gcccgatgtt tcttcagccg
     1381 gagcaaccgg ccgcttgcct ctcggcggaa gtcccggctc cggcggcgca tttcagattg
     1441 caggcgccgg catcccgccg gtcgagcggc cgcgcgcgcg aaccaatgtc gccagtcccg
     1501 cctatttcgc ctccatgggc attccgctcc ttcgcggacg cgatttctcc tggaccgaag
     1561 atcgtcccgg cggacgccgc gttgtaatcg tcaatcaagc ctttgccagc ggatttttgc
     1621 gcgaccgcga tcccatcggc gcttcgctgg agttgggctg gacaagcgag ctcaacccgc
     1681 gcaattcgct ttgggaagtc gttggcgtgg ccggaaacac tcgccagaat tccatggacc
     1741 gtaatccggc cccggaaatc ttcctttcca tgacgcagac gggcgcggat ggcgcgggct
     1801 acgtcatccg agctcgcgat ggccgcggcg atatcgcgcg agtcatcgcc gaaaccgtcg
     1861 tgcagttcga cccgcgcgtt cagcgcgtgc atcccacgcc gctggtcacg ttgatcgagc
     1921 gcaatctcga tagccgcgcc accgccatca aattgatcgg cggtttcggc gcagtcgcgc
     1981 tattgctcac ggccgcgggc atctttggaa ttgtcgcgtt tcatgcacag gagcgctcgc
     2041 gcgaaatggc catccgcatg gctctcggct cacccactgc tgatgttcaa cgtctcatca
     2101 ttctccaagc cctgcgactc gcaggcgttg ggtcattgat aggcgtgttc ggattttggt
     2161 ggggcagtcc gtggttgcgc aatcaacttt atggggtcgg ccgcgccgat gctgctacgt
     2221 tcgccggcgt tctggcgcta gtagccgccg tcgccgtttc tgcttcgctt gcgccatcgc
     2281 gccgcgcctc ccgcaccgac ccggcgcgcc tgttgcgcga aggctgacgc gcagtcgaac
     2341 tgccgatcat tcaaagaatc gcgcaatgcg cgcgcgcaat cgttcggggt ctttcaactc
     2401 gcactcccaa accaccagca tgcgccatcc caggctccgc aagcgccgcc ggttggacga
     2461 atcgcgcgcc acattccggt tgagcttcgc aatccagtaa tcggtgttcg atgccggccg
     2521 gttgcgtccc gaacggcacg agtgtccatg ccagaagcac ccgtgcacga agatgatcct
     2581 ctgccgggac ggaaagacca tgtcgggatt ccctggaaga tcgcgccggt gcagcgcgta
     2641 gcgaaatccc atgcgatgca ccatgctgcg gaccgccatt tccggctgag tatccttgct
     2701 gcgcacccgc ttcatgaccg cgctgcgctc ggccgccgaa aacgtgtcca ttctccccga
     2761 tgatatccct caaggcgcgc gcggcgccca gccgtttatc gctgtaaatc gcccgtttcg
     2821 tgcactatgc cacggcgcgg ctccgcctgg ggacgataga atcgcagcgg gagaccgttc
     2881 atgacgccga atccaccaat tgaagacgca gacgttcata ttccaactcg ggacatcgat
     2941 attcccgctt atcaggcgtt gcccgccgct ccaggtccct tccccatcgt gctgattgcc
     3001 ccggaaatct ttggcgtgaa cgaacacatt cgttccgtcg cgcgacgctt cgcttccgag
     3061 ggctactatg ccatcgcgcc ggatctcttt atccgccagg gcgacgtttc gaagctcacc
     3121 gacagccagg aaattcaaca gatcatcgcc aaggtgccag atatgcaggt gatgagcgat
     3181 ctcactgccg ccgccgagtt cgcctcagcc agcgggcgcg gcaacatcga gcgtctcttg
     3241 atgaccggat tctgctgggg tggccgtatt gcatggcttt atgccgcgga aaacagccac
     3301 ctgcgcgccg ccgtcgcttg gtatggacgt ctcaccgccc cgcttgatgc gctgcacgaa
     3361 cggcatcctt tgaatatcac tgacgacctg aagtgtcccg ttcttggcct gtatggcggt
     3421 caggacaaga gcatcccgct cgaacacgtc gagcgcatgc gcgccgaaat tgcgcgatcg
     3481 cag
//
LOCUS       DASZMC010000471         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1003288,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000471 DASZMC010000000
VERSION     DASZMC010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003288"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VG274_09775"
     CDS             <1..1527
                     /locus_tag="VG274_09775"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWE06986.1"
                     /translation="PPCGQGAMAQTILIVDDDPVQRRLLEAAITRAGMTVVTAPGGQP
                     ALDLLNGPRGDQFTLVLLDLVMPDMDGLTVLAKLKVSHRDLPVIVLTAKGGIDSAVEA
                     MRAGASDFLVKPASPERITVSIRNALKIGTLAGEVTRLKKRADNRLDFDDLIGVSAEM
                     NQVVRLGRRAAQSNIPILIEGESGVGKEVIARAIQGSSERAGRPFVTVNCGAIPENLI
                     ESILFGHEKGSFTGATDKHLGKFQEAGGGTLFLDEIGELRLDMQVKLLRALQEGEIDP
                     VGSKRPVKVDVRIISATNRDLAERTREGLFREDLYYRLNVFPIYVPALRQRREDVAEL
                     ARYFIEKFAAEENKPIGGVTPEAGGILERFDWPGNVRQLENTIFRAVVLCDGATLDVC
                     DFPQLAAAMGVETKLRKAPIAVPPLRILEPVKEIVSVAQVSGVAIAATNEFGHVRRLE
                     DIEGDLIRMAIDHYDGHMSEIARRLGIGRSTLYRKLRELGLDQPESAEPEEERIREAV
                     "
BASE COUNT          318 a          480 c          481 g          276 t
ORIGIN      
        1 ccaccatgcg gtcaaggcgc aatggcacag accatcctca tcgttgacga cgatcccgtt
       61 cagcgcaggc tgctcgaggc ggcgatcacg cgcgccggaa tgacggtcgt caccgcgccc
      121 ggcggccagc cggccctgga cctgctgaac gggccgcgcg gagaccagtt cacgctcgtc
      181 cttctggatc tggtgatgcc ggacatggac ggtcttaccg tgctcgcaaa actcaaggtg
      241 agccatcgcg atcttcccgt catcgtactg acggccaagg gcggaatcga ttcggcggtt
      301 gaagcgatgc gtgccggcgc gagcgatttt ctggtgaagc ctgcaagccc cgaacggatt
      361 acggtctcga tccgcaatgc gctgaagatc ggcacgctgg ccggagaagt gacgcggctc
      421 aagaaacgcg cggacaaccg tctcgatttc gatgatctca tcggcgtgag cgcagagatg
      481 aaccaggtcg ttcgccttgg ccggcgagcc gcacaatcga acattccgat tctgatcgag
      541 ggcgagtccg gcgtcggcaa ggaagtcatc gcccgcgcca tccaggggtc ttccgagcgc
      601 gcgggccggc cattcgtcac ggtgaattgc ggcgccattc cggaaaacct tatcgaatcg
      661 atcctgttcg gccacgagaa aggttcgttc accggcgcaa ccgacaagca tctggggaag
      721 ttccaggaag caggcggcgg caccctgttc ctcgacgaga tcggcgaact tcgcctggac
      781 atgcaggtca agcttctgcg cgctttgcag gagggcgaga tcgaccctgt tggctcgaag
      841 cgcccggtca aggtagatgt tcgcatcatt tcggcgacca accgcgatct cgcggaacgc
      901 acgcgcgaag gtctgttccg cgaggatctc tattaccggc tgaacgtctt tccgatctac
      961 gtgccggcac tgcggcaacg ccgcgaggac gtcgcggaac tggcgcgata cttcatcgag
     1021 aaatttgccg cggaagaaaa caagccgatc ggaggcgtaa ctccggaagc aggcgggatt
     1081 ctcgagcgct tcgattggcc cggcaacgtc cgccagctcg aaaacacaat cttccgcgcg
     1141 gtcgtgctgt gcgacggcgc cacgctcgat gtctgcgatt tcccacagct cgctgccgca
     1201 atgggcgtcg agacgaagct gaggaaagcg cccatcgccg ttcctcccct tcggatcctg
     1261 gagccggtga aggaaatcgt ttccgttgcg caggtctccg gcgtcgcgat cgcggcaacg
     1321 aacgagtttg ggcacgtgcg caggctcgaa gacatcgagg gcgacctgat tcgcatggcc
     1381 atcgatcact atgatggaca catgtcagaa atcgctcgcc gcctcggcat cggacgttcg
     1441 acactctacc gcaaactcag agagctgggt cttgatcaac cggaaagcgc cgaacccgaa
     1501 gaagaacgca tccgcgaagc cgtttgacgg agatgcaacc gacgcgttac ttcgc
//
LOCUS       DASZMC010000472         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_189811,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000472 DASZMC010000000
VERSION     DASZMC010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189811"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VG274_09780"
     CDS             <1..664
                     /locus_tag="VG274_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE06987.1"
                     /translation="IRPDFLPYVFDRFRQADSSTTRRHGGLGLGLSIVKSLVELHGGC
                     VRAESGGEGLGSAFFVTLPIGPELDHLAPASTDLASAADADRVFASFGLTGVRVLVVD
                     DEQDARDLARRLLEECHAHVATAASVEEALLQFNAVLPDVLICDIGMPGQDGYDMIRR
                     VRGRSPEAGGIVPAAALTAFAREEDRRRAMSAGYQAHIAKPVDAISLLSAIARLAGRA
                     RA"
     gene            complement(1002..>1831)
                     /gene="nadC"
                     /locus_tag="VG274_09785"
     CDS             complement(1002..>1831)
                     /gene="nadC"
                     /locus_tag="VG274_09785"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWE06988.1"
                     /translation="TSRLMVPESAVGVGTLVQKEVGIACGLPIVEMVCRTYDERLRVE
                     LIPGFHLEIIEGRFSDAQKTPLLRLRGPLRSLLSAERVILNFLQRMSGVATHTHRYVR
                     RTTGTGAKIFDTRKTIPGHRLLDKYAVRCGGGFNHRIGLFDGLLVKDNHVAAVHLKEL
                     QAYLTKVVATCRAEDASRLIEIEIDNLEQLREVIKVDGVQVILLDNMDCPKMELAVEI
                     RNKAGKKGIVELEASGGVTLETIRRIAETGVDRIAVGALTHSAPALDISLEVEESVQ"
BASE COUNT          331 a          566 c          558 g          376 t
ORIGIN      
        1 aatccgcccc gatttcctgc cttatgtttt cgatcgcttt cgccaggcgg attcttcgac
       61 gactcgccgg catggcggac tgggcctggg gctgtcgatc gtcaaaagcc tggtcgaact
      121 tcacggcgga tgcgttcgcg ccgagagcgg cggcgaagga ctcggatcgg cattcttcgt
      181 cacgcttcct atcggcccgg aactggatca tctcgcgccc gcctcgaccg atctcgccag
      241 cgccgccgat gccgatcgcg tatttgcttc attcggcctg accggcgtgc gcgtgctggt
      301 ggtcgatgac gaacaggatg cacgcgacct ggcccgccgg ttattggaag aatgccatgc
      361 ccacgttgca acggcggcgt cggttgaaga ggcgctgctg cagtttaatg cggttctgcc
      421 cgatgtgctg atctgcgaca tcgggatgcc gggccaggat ggctacgaca tgatccgccg
      481 cgtgcgcggg cgctcgccgg aagccggcgg gatcgtgccg gcagccgcgc tcactgcctt
      541 cgcgcgggaa gaagaccgcc gccgcgccat gagcgccggt tatcaggccc acatcgcaaa
      601 gcccgtggat gcgatcagcc tcttatccgc gatcgcgcgc ctcgcgggcc gcgcccgggc
      661 atgacgaaat ctttgcccgg acatgacgaa gactttgccc gggcatgacg aagactttgc
      721 cgcgaagatg cgaagggacg aagagcttct cgaacttcgg gcacaaatcc gtgatatttg
      781 tttatattta agggcaggct ggcgtgaacc gagataaggc gctgtgcatg aataagaccg
      841 catttctgta gggtgggtgt actcgcccac caatgcgcca cgtgcgcgga aacaaacggt
      901 gggcgagtac acccacccta cagaaatgcg gatttacttt tgcacgcctc ctaagcgtgc
      961 gatgccacgg ggcttgttac gttttagtca ttactcattt ctcattgaac agactcctcc
     1021 acctccaggc tgatgtccag cgccggcgca gaatgggtca gtgcgccgac ggcgattcga
     1081 tcgacgccgg tctcggcgat gcgccggatg gtttccagcg tcacgccgcc ggaggcttcg
     1141 agctcgacga tgcctttctt ccctgccttg ttgcgaatct cgacggctaa ttccattttc
     1201 gggcagtcca tgttgtccag gagaatcacc tgcacgccgt cgactttgat cacctcgcga
     1261 agttgttcga ggttgtcgat ttcgatctcg ataagccgcg atgcgtcttc ggcccggcag
     1321 gtggcgacga ccttcgtcag ataagcctga agctccttca agtggacggc ggccacatgg
     1381 ttgtctttga cgagcagtcc gtcgaacagc ccgatgcggt ggttgaatcc tccgccgcag
     1441 cggacggcgt atttgtcgag caggcgatgg ccggggatgg tcttgcgcgt gtcgaaaatc
     1501 ttggcgccgg ttcccgtcgt gcgacggaca tatcgatgcg tgtgcgttgc cacgccgctc
     1561 atccgctgca ggaaattcag gatcacgcgc tcggctgaga gcaacgaccg caacggcccg
     1621 cgcagccgca agagcggcgt cttctgcgca tcgctgaatc tgccctcgat gatctccaga
     1681 tgaaagccgg gaatcagttc gacccgcagc cgctcgtcat aggtccggca gaccatctcg
     1741 acaatcggca acccgcaagc gatgccgact tccttctgca ccagcgtccc gacgccgacg
     1801 gcgctttccg ggaccatcag ccggctggta a
//
LOCUS       DASZMC010000473         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2402276,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000473 DASZMC010000000
VERSION     DASZMC010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402276"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..>1729
                     /locus_tag="VG274_09790"
     CDS             277..>1729
                     /locus_tag="VG274_09790"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="HWE06989.1"
                     /translation="MQFLKTLFAHTTNPIYVCSFPNERNDESQVGERHVITRTPAHIT
                     KFVAKWDRKKRGLFFGVGTVQASASTRKKETIAETIGLHVDIDFKHVDLNYTRDQIVR
                     KLTELKYPPSAIVFSGGGLHCYWLFKEPLETQGNIERIELALHQLADLVAGDPQCCEV
                     SRVLRVPGSHNTKDGAWTEVEVLALNDLRYELDDLEEWLSEQSPILLRKSRERGRTVG
                     EIEEDFFARFAKEHGIKPPIDVDRRLKDMIYMGAGDSSIHQTQIQCAASMLSRGMAVD
                     DVVAILLAATKVAAGDYGTRWNWRIEERNIRRDCVGWIKKHPPEKKPKAEAKAKPKAE
                     SSDGGAAGAQEQEQEETAQAASGGAGSGNAGAGAKTDDDNVVEFPQPGVSKGELHIAL
                     GRAVLAHMRAQGEALVNGRDGSWLYAAGIWELRVDIKAWLDARIEEVCRGLGFKSNNK
                     LVNETRCWIIRNPELRRDEIPWDQHGKIPTRSGL"
BASE COUNT          378 a          516 c          548 g          287 t
ORIGIN      
        1 gaagcgcgtc cacatcagcg accgccgtgt tggctggatg gtcgaggaga tcgatgcgca
       61 tgtcgctgcg aaaatcgcca agcgtcacgc cagcgtcgtc tctaacaaac ccgccgcagc
      121 ggcctagcaa aaaaacccca gcgctgccgc gctggggttt gaacaactgg aaccactcga
      181 atgctttcct ataacgatga caccgcgcac gcgcaagcgg ttagcagcga cgatcccggg
      241 ctgaagccga ccggcgagcc gcgaccgaac ctgctcgtgc agttcctcaa gacactgttc
      301 gcgcacacga ccaacccgat ctatgtctgc tcgttcccga acgaacgcaa tgatgaaagc
      361 caagtcggcg agcgtcacgt catcacgcgc acgcccgcgc atatcacgaa attcgtcgcc
      421 aagtgggacc gcaagaagcg cgggctgttc ttcggcgtcg gcaccgtgca ggcaagcgcc
      481 agcacgcgga agaaagagac catcgccgag acgatcgggc tccacgtaga catcgatttc
      541 aaacacgtcg atctcaacta cacgcgcgac cagatcgtgc gcaagctgac ggagctgaag
      601 tatccgccgt ctgcgatcgt gttctcgggc ggtggcctgc attgctattg gctgtttaag
      661 gagccgctgg agacgcaggg caacatcgag cgcattgagc tggccctgca tcaacttgcc
      721 gatctcgtgg ccggtgaccc gcagtgctgc gaagtctcgc gcgtgttgcg cgttcccggc
      781 tcgcacaata ctaaggacgg cgcatggacc gaggtcgaag ttctggcgct gaatgatttg
      841 cgctatgaat tggacgatct tgaagaatgg ctgagcgagc aatcgccgat cttactccgc
      901 aagagccgcg agcgcggcag gacggtcggc gagatcgaag aagatttctt cgcgcgcttc
      961 gccaaggagc acggcatcaa gccgccgatc gatgtcgatc ggcgcttaaa agatatgatc
     1021 tatatgggcg ctggcgatag ctcgatccat cagacgcaaa tccagtgcgc cgcctcgatg
     1081 ctcagtcggg gtatggccgt tgatgatgtc gtggcgatcc tgctggccgc aaccaaagtc
     1141 gccgctggcg actatggcac ccgttggaat tggcgcatcg aggagcgcaa catccgccgc
     1201 gattgcgtag gttggatcaa gaagcatcca cctgagaaga agccgaaggc tgaggccaaa
     1261 gccaaaccca aggccgaaag cagcgacggc ggcgcggctg gggctcagga gcaggaacag
     1321 gaggagaccg cgcaggcggc ttctggcggc gctggcagcg gcaacgccgg cgctggcgcc
     1381 aagactgatg acgacaacgt cgtggaattt ccgcagccgg gcgtgagcaa gggcgagctt
     1441 catatcgcgc tcggccgggc cgtgctcgcg catatgcgtg cgcagggcga ggcgctggtc
     1501 aacggccggg acggatcgtg gctttacgcg gcgggcatct gggaattgcg cgttgacatc
     1561 aaggcgtggc tcgacgcgcg catcgaggag gtgtgtcgcg gcctcggctt caagagcaac
     1621 aacaagctag tcaacgagac ccgctgttgg atcatccgca atcccgaact ccggcgcgat
     1681 gaaatcccat gggatcagca cggcaaaatc ccaacgcgct ccggcttgg
//
LOCUS       DASZMC010000474         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_764373,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000474 DASZMC010000000
VERSION     DASZMC010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764373"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1372)
                     /locus_tag="VG274_09795"
     CDS             complement(116..1372)
                     /locus_tag="VG274_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWE06990.1"
                     /translation="MLTDVAIKALRSKEKDYKVADRDGMYVHVTTKGALSFRMDYRLN
                     GRRETIYLGKYGPDGLSLARAREKCIDARRAIADGISPAIEKQREKRRLKESKSFGEF
                     GEKWLTAAPMADSTRAMRRSIFERELLPVWRNRLLTEISPDDLRAHCASIVDRGAPAT
                     AIHVRDILKQIYGFAILHGEKVANPAEEVGPASIATFVPKDRSLSPSEIRVLFKVLEK
                     VPTLPTIRLGMRFFLVSMVRKSELQDAIWDEVDFENAVWTIPKERMKRSKAHNVYLSR
                     QMLDILIALKTCAGNSRYLLPSRYDADAPMSRATFNRVTYSVVEQAKQEGLPLEPFTV
                     HDLRRTGSTLLNELGFNSDWIEKCLAHEDGRSSRGVYNKAEYEVQRRHMMQEWSDIVD
                     AWINGRKYVPTLIPPSMPVLEPDPAL"
     gene            complement(1665..3212)
                     /gene="guaA"
                     /locus_tag="VG274_09800"
     CDS             complement(1665..3212)
                     /gene="guaA"
                     /locus_tag="VG274_09800"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWE06991.1"
                     /translation="MSAPVLVIDFGSQVTQLIARRVREGGVYCEIVPFNKADEAIEAA
                     PPKAIILSGGPASITEAASPRAPARAFELGVPVLGICYGQQAMVDQLGGRVEGSSKRE
                     FGRATIEVSGQSPLFDGVWALGARDDVWMSHGDRVTALPNGFHVIAESENAPYAVIAD
                     ERRRFYGVMFHPEVVHTPRGAALLTNFVQNIAGIAPDWNMKTFEREAIARIRMQVGRG
                     RVICGLSGGVDSSVAALLIHEAIGDQLTCIFVDHGLLRAGEAEEVVSLFRGHYNIPLV
                     HVDAAELFLGRLAGVDEPEKKRKIIGASFIDVFEQEAGKVGGAEFLAQGTLYPDVIES
                     VSPLGGPSVTIKSHHNVGGLPARMRMKLVEPLRELFKDEVRALGRELGLPETFVGRHP
                     FPGPGLAIRIPGEVTHEKLEVLRRADTIYLEEIRKAGLYDEIWQAFAVLLPVRTVGVM
                     GDDRTYDNVCALRAVTSRDGMTADSYPFDHAFLARVATRIVNEVKGINRVVYDVTSKP
                     PGTIEWE"
     gene            complement(3432..>3630)
                     /locus_tag="VG274_09805"
     CDS             complement(3432..>3630)
                     /locus_tag="VG274_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE06992.1"
                     /translation="LACENADRVAAFLSRHPEFTVIPAAQIWHDATALPPPPGMAEFL
                     KATPRATGTDGFFAAVLIRRG"
BASE COUNT          683 a         1193 c         1090 g          664 t
ORIGIN      
        1 ccgcgttgcg ttgtctggga tctggcggag gtcgaggcgt ggctcgcggc gcgccgatca
       61 gttccgatcg cgcgcgccaa acaccctgtc gttcagcagc gccgcgcccg ccccgtcaaa
      121 gcgccgggtc gggctcaaga accggcatcg agggcgggat aagcgtcggc acgtactttc
      181 gcccgtttat ccaagcatca acgatgtccg accattcctg catcatgtgc cgacgctgca
      241 cctcgtattc ggccttgttg tagacgccgc gagaggatcg gccgtcctca tgcgcaaggc
      301 acttctcaat ccagtcgctg ttaaacccca gctcattcaa gagcgtcgac ccggtccgtc
      361 gcagatcgtg gaccgtgaat ggctcaaggg gcaggccctc ttgcttcgcc tgctcaacaa
      421 cggaataggt cacacgattg aaggtcgccc gggacatcgg cgcgtccgca tcatatcgcg
      481 agggaagcag ataccgtgag tttcccgcac aggtcttcag ggcaatgaga atgtcgagca
      541 tctgacgcga caggtaaaca ttgtgcgctt tcgagcgctt cattcgctcc ttcggaatcg
      601 tccagacggc attctcaaaa tcaacctcgt cccagatcgc atcctgtagt tcgctcttgc
      661 ggaccatcga cacaaggaag aaccgcatcc ccagccgaat ggtgggcagc gtcgggactt
      721 tctctaagac tttgaacaga acccgaatct cggaaggaga cagcgaccga tccttaggca
      781 cgaaggtcgc aatcgacgca gggccaacct cctccgcggg gttggcaacc ttctcaccgt
      841 gaaggatcgc aaacccgtag atctgcttca ggatatcccg cacgtggatg gccgtcgctg
      901 gcgcgccccg atcgacgatg ctggcgcagt gcgctcgcag gtcatccggg gaaatttccg
      961 tcagcagcct gttgcgccaa accgggagca gctcccgctc gaagatcgag cgtcgcatcg
     1021 cgcgcgtgct atccgccatc ggcgccgcgg tcagccactt ctccccaaac tccccaaagc
     1081 tcttggactc ctttaggcgc cgcttctcgc gctgcttttc gatcgccggc gagatcccat
     1141 cggcaatggc gcgcctcgcg tcgatgcact tctctcgtgc ccgggcgagc gacaatccgt
     1201 cgggtccata tttgccgaga tagatcgtct cccggcgccc gttcagacgg taatccatcc
     1261 gaaaagacag cgcgcccttg gtcgtaacat ggacatacat cccgtcgcga tccgcgacct
     1321 tgtagtcttt ttccttggat ctcagagcct taatcgcgac gtccgtcagc atcgcacacc
     1381 tcgccagaaa actaccgtca ggcaatctca gcgcgccaac gatcagaaaa cgtgtgtatt
     1441 ttcagatcac tagacgaaag aaactaccgt cagaggcccg cgtgaccatg acggtagtcg
     1501 cgattgtgat gagaggcaat agggtcgacc accgtcagcc gtcccgtcag atcggatttc
     1561 tgcaccccga tagccaccga taggtggtgc catatttatt tatctctttc aatggtttat
     1621 ggcgttttca gggatagcct gggatacccc aggatgggca aaaatcattc ccactcgatc
     1681 gtacctgggg gtttcgacgt cacgtcatag accacgcggt tgatgccttt gacctcgttc
     1741 acaattctcg tagcgacgcg ggcgaggaag gcgtggtcga acggatagga atccgcggtc
     1801 atgccgtccc tcgacgtcac cgccctgagc gcgcagacat tgtcgtaggt gcggtcgtcg
     1861 cccatcacgc ccacggtgcg gacggggagc agcaccgcga aggcctgcca gatctcgtcg
     1921 tacaagccgg ctttgcggat ttcttccaga tagatcgtat ccgcgcgccg caaaacttcg
     1981 agtttctcgt gggtcacttc gccggggatg cggatcgcca gtccgggccc cgggaaggga
     2041 tgccggccaa cgaaggtttc cggcaagccg agctcgcggc cgagcgcgcg cacctcgtcc
     2101 ttgaacaatt cgcgcaaggg ctcgaccagc ttcatgcgca tgcgcgccgg caacccgccc
     2161 acattgtgat gcgattttat ggtgaccgac ggcccgccga gcggcgacac gctctcgatg
     2221 acgtccgggt agagcgtgcc ttgcgcgaga aattccgcgc cgcccacctt cccggcttcc
     2281 tgctcgaaca cgtcgatgaa ggaggcgccg atgatcttgc gcttcttctc cggctcgtcg
     2341 acgcccgcga gccggccgag gaacagctcc gccgcgtcca catggacgag cggaatgttg
     2401 tagtggccgc ggaacagcga gacgacttcc tccgcctcgc ccgcgcgcag gagtccgtga
     2461 tcgacgaaga tgcaggtgag ctgatcgccg atcgcttcgt gaatcagcag cgctgcgacc
     2521 gaggagtcga caccgcccga gagtccgcag atcacccgcc cgcggcccac ctgcatgcgg
     2581 atgcgcgcga tcgcctcgcg ctcgaacgtt ttcatgttcc agtcgggcgc gatgccggcg
     2641 atgttctgca cgaaattcgt cagcagcgcg gcgccgcgcg gcgtgtgcac cacttcggga
     2701 tggaacatca cgccatagaa gcggcggcgc tcgtcggcga tgacggcgta gggcgcgttt
     2761 tcgctttcgg cgatgacgtg aaacccgttg ggcagcgcgg tcacgcggtc gccatggctc
     2821 atccacacat cgtcgcgcgc gccgagcgcc cacacgccgt cgaacagcgg gctttgacca
     2881 ctcacctcga tcgtcgcccg cccgaactcg cgcttcgacg agccttcgac gcggccgccc
     2941 agctgatcga ccatcgcctg ctggccatag cagatgccga gcaccggcac gcccagttcg
     3001 aacgcccgcg ccggagcgcg cggcgaagcg gcttcggtga tgctcgcggg tccgccggaa
     3061 agaatgatcg ctttgggcgg cgcagcctcg atcgcctcgt ctgctttatt gaaagggacg
     3121 atctcgcaat agacgccgcc ctcgcgcacg cgccgcgcga tcagctgcgt cacctgcgaa
     3181 ccgaaatcga tgacgaggac gggagcggac atgacgctaa catcctcccc ggggcttcgc
     3241 agcgggcccc ctccaccgct tcgcggtccc cctcccccac gcttcgcgcg ggggaggaaa
     3301 cacgcttctc tctttcctcc ctcgccgaag gcgggggagg tgcccgaagg gcggaggggg
     3361 caacgcggaa tcggtggaaa acatgccatc acctaatggc acgccaccac cttgtccaga
     3421 agcggggact atcacccgcg ccgaatgagc acagccgcga agaatccgtc cgtgccggtg
     3481 gcgcgcggcg tggctttgag gaattcggcc atccccggcg gcggtggcag agcggtggcg
     3541 tcatgccaga tttgggcggc cgggatgacc gtgaactccg ggtggcggct gagaaaagct
     3601 gccacgcggt cagcgttctc gcaggcaagc
//
LOCUS       DASZMC010000475         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2402526,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000475 DASZMC010000000
VERSION     DASZMC010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402526"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1869)
                     /locus_tag="VG274_09810"
     CDS             complement(193..1869)
                     /locus_tag="VG274_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWE06993.1"
                     /translation="MSARNIIRGTAARTVEALAGPGVAPDIVAGLTLAAIAVPEQMAT
                     ARLGHFSPEIGFLAFVAGTLGFIALGASKTVSVGADSTITPIFAASLALIVAAGSPAY
                     AALAPMLALMVGAIVAVSGLCRMGWISNLLSVPVTAGFLAGISVHIAASQLPVLCGLP
                     AGAGGTIARLVSLAGNLGQANPYAVLLGLGVLAITALTERLGPQWPGALIGVAAATLV
                     TLVFALERNGVTVLGAISPPVPRFPTYWPTVEEISRLAPLSFLVAIVVMVQSAATTRS
                     FPSGSSPDIDRDFVGVGVANLVSGLVGGFPVNASPPRTAIVAEAGARSKRAGLVAAGA
                     VTLLAVYGSTLLAHVPHAALAGVLLFVALRILQLRVASAVWRESKGEFALILATAFAI
                     VALPIETGVAIGIVLSLLHGMSTTTRARVIELVRVPGTSIWWPPAENTRAGETAGVLV
                     LAFQAPLSFLNANIFRRGILDAVARAPAPVRLVVLEAGSIVEIDFTAAHVMSEVVRAC
                     RDKGALFAIARLESVRAQHSLRRFGIVDLVGAAHIYRSVYEAVEALAPSG"
     gene            1901..2524
                     /locus_tag="VG274_09815"
     CDS             1901..2524
                     /locus_tag="VG274_09815"
                     /inference="COORDINATES: protein motif:HMM:NF020432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HWE06994.1"
                     /translation="MPVVREPVLKPIPLADLRPTQMTLGLREVEERRKQWRARKGKKE
                     NSFFGTHLIPVVLGPKGRHYVIDHHHLARALLDEGMRDIFVTVVARIEKLSEDAFWVV
                     LDNLGWMHPFNAKGRRCGYGDIPGSIKDMEDDPFRSLAGALRRAGGYSKETTPFSEFL
                     WADFLRRRMKRQIVKHHFNEAMRKALSLAKSKDADYLPGWCGAVDRP"
     gene            2616..3665
                     /locus_tag="VG274_09820"
     CDS             2616..3665
                     /locus_tag="VG274_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWE06995.1"
                     /translation="MQLLGKQSKARLAMAAIVLALCIGMFDYTTGTHISLSAFYLLPV
                     SLAAWFVGTGFALFIAALCVAVWMIGNLGAGNADFADPFLTAWNGSVQLMSFVVVVLV
                     LVKLRSLNHLLESRIRERAAALTREIAERERLQHLLLEASEQEQRRIGQDLHDGLGQH
                     LAGTAIAGQVLREKLERRRLHEAADAKKVVELIEEGVSLTRRSAKGLHPVDLSAEGLM
                     LALEEFAVTTTRLFEVNCRFVCELPVLVHSAATAEHLFRIAQEATRNAIQHGGARDIA
                     IELNTLDEGHELRIEDDGAGIPANRPSTAGMGLRIMAHRARLIGATFEVGARDGGGMI
                     VTCRLPVAIEDQIAS"
BASE COUNT          762 a         1183 c         1233 g          642 t
ORIGIN      
        1 tttcagtctc actgaacgcg ccgtttcctg tcattcctcg ttccggatgc acgatgtgcc
       61 gaagagcata tctgctggct atacccttgg cgtaagtaat cggtgtctcg ttccgcatcc
      121 gccacctctt gccgcgaaaa aaacaaacga acgcgcgcaa accctccctt tggcgacaac
      181 cattgacgtc cgctatccgg acggcgcgag agcttcgacc gcttcgtaaa cactgcgata
      241 gatgtgcgcg gcgccgacga ggtcgacaat gccgaagcgg cgcaatgaat gttgtgcccg
      301 gaccgattcg aggcgggcga tcgcaaaaag ggcgcccttg tcgcggcagg ctctcacgac
      361 ctcggacatg acgtgggcgg ccgtgaagtc gatctcgacg atgctgccgg cctcgagaac
      421 gacaaggcga acgggcgccg gcgcacgcgc caccgcatcg aggatgccgc ggcggaagat
      481 gttcgcattg agaaatgaca acggcgcctg aaaggcaagg acaagaaccc cggcggtttc
      541 cccggcgcgc gtgttttcgg caggcggcca ccagatggaa gttccgggca cgcgtacaag
      601 ctcgatcaca cgggcgcgcg tcgtcgtcga catgccgtga agcagggaca gaactatgcc
      661 gatcgcaacg cccgtctcga tgggaagcgc tacgatggcg aacgcggtcg ccagaataag
      721 cgcgaattcg cccttgcttt cgcgccagac cgcgctggcg accctgagct gcaggatgcg
      781 caacgcgacg aagagaagca cgcccgccag tgcggcatgg ggaacatgcg cgagaagagt
      841 cgagccgtag accgcaagca gcgtcaccgc tccggccgcg accagtccgg ctcttttcga
      901 tcgggcgccg gcttcggcga cgatcgccgt ccgcggcggg ctggcgttga ccggaaaccc
      961 gccgacgagt ccggaaacca gattggccac gccgacaccg acgaaatcac gatcgatgtc
     1021 gggtgacgaa ccggatggaa aggatcgcgt cgtggccgcg gactggacca tcaccacgat
     1081 ggcgaccaga aacgatagcg gagcgaggcg cgagatctct tccaccgtcg gccagtaggt
     1141 ggggaagcgc ggcactggcg gcgaaattgc tcccaacacc gtcaccccat tgcgctccag
     1201 ggcgaaaacg agcgtgacga gcgtcgcggc ggcgacaccg atcaacgccc cgggccattg
     1261 ggggccgagc ctttccgtca gcgcggtgat cgcgagcacg ccgagcccga gcaggaccgc
     1321 gtaggggttc gcctgcccga gatttcccgc gaggctcaca agccgtgcga tcgtgccacc
     1381 cgcgccggcg ggaaggccgc acaggaccgg aagctgcgat gcggcgatgt gcacggatat
     1441 tccggccaga aaccccgcgg tgacggggac cgaaagcaga ttggaaatcc agcccatgcg
     1501 gcagaggccc gacaccgcaa cgatcgcccc gaccatgagc gccagcatgg gcgcgagcgc
     1561 tgcgtaggcg ggcgatccgg ccgccacaat gagtgcgagg ctggccgcga aaatgggggt
     1621 gatcgtcgaa tccgcgccga cggaaaccgt cttgctggcg ccgagcgcga tgaagcccag
     1681 cgtgcccgcg acgaaggcga gaaacccgat ctcgggcgaa aagtgaccga gccgcgcggt
     1741 tgccatctgt tccggcacgg cgatcgccgc caaagtaagg ccggcaacga tatccggcgc
     1801 gacgcccggg ccggccaagg cttccacggt gcgcgcggca gttccgcgga tgatatttct
     1861 tgccgacatg gttttgattc gtccccgagg agtctagccg atgccggtcg tacgcgaacc
     1921 cgtcctcaaa cccataccgc tcgccgatct caggccgacg cagatgaccc ttggcctgcg
     1981 cgaagtggaa gagcgccgca aacaatggcg cgcgcgcaaa ggcaagaaag agaacagctt
     2041 cttcggaacc cacttgatcc ccgtcgtgct cgggccaaaa gggcgtcact atgtgatcga
     2101 tcatcatcac ctcgcgcggg cgcttctcga cgagggaatg cgcgatatct tcgtgacggt
     2161 agtcgcccgc atcgagaagc tgagcgagga tgcgttttgg gtcgtgctcg acaatctggg
     2221 ctggatgcat cccttcaacg cgaaggggcg cagatgcgga tacggggaca tacccggctc
     2281 gatcaaggat atggaggacg atccgtttcg ctcgctcgcc ggtgctctcc gccgcgccgg
     2341 cggctactca aaggaaacca cgccgttcag cgaatttctg tgggcggatt tcctgcgtcg
     2401 gcgcatgaag cggcagatcg tcaagcacca tttcaacgag gcaatgcgca aggcgctatc
     2461 tctcgcgaag agcaaggacg ccgattatct tcccggctgg tgcggcgccg tcgaccgacc
     2521 gtgaccatct ggcggatgga tttctcgcgc attgcggcgc acgatcaggc cggtattcaa
     2581 gccgggagag gttcaatcca cagatgggga cgtggatgca gcttctgggg aagcaatcga
     2641 aggcgcgtct cgccatggcg gcgatcgttc ttgcgctgtg tatcggcatg ttcgattaca
     2701 cgacgggcac gcatatctcg ctttccgcct tctatctcct tcccgtttcc cttgcagcat
     2761 ggttcgtcgg caccggcttc gcgttgttca tcgcagcctt gtgcgtcgcc gtctggatga
     2821 tcggcaattt gggtgcgggg aatgccgatt ttgccgatcc atttttgact gcctggaacg
     2881 gcagcgtaca attgatgtct ttcgtggttg tcgtgctcgt cctcgtcaaa ttgcggagcc
     2941 tgaaccatct tctcgaatct cgcattcgcg agcgggcggc ggcgctcacg cgcgaaatcg
     3001 ccgagcggga gcggctgcag cacctgttgc tcgaagccag cgaacaggag cagcggcgca
     3061 tcggtcagga tctgcacgat ggtctgggcc agcacctggc gggcaccgcc atcgccggcc
     3121 aggtgttgcg tgaaaagctc gagcgccggc gtttgcacga agcggccgat gcaaagaaag
     3181 tcgtggaact gatcgaagaa ggcgtctcgc tcacgcgccg ctccgccaaa gggttgcatc
     3241 ctgtcgacct gagcgccgaa ggcctgatgc ttgcgcttga agaatttgcg gtcacgacca
     3301 cgagactgtt cgaagtgaac tgccgattcg tttgcgaatt gcccgtcctc gtacactcgg
     3361 cggcgacggc cgaacatctc ttccggatcg cccaggaggc cacccgcaac gcaatacagc
     3421 atgggggcgc gcgcgacatt gcaatcgaac tcaacacgct cgatgaaggt cacgaattgc
     3481 gcatcgaaga cgatggcgcc gggattccgg cgaaccggcc ctcgaccgcc ggtatggggt
     3541 tgcgtatcat ggcgcaccgg gcgcggctga tcggcgcgac cttcgaggta ggggcacgcg
     3601 acggcggcgg aatgatcgta acctgccgct tgccggtcgc aatcgaggac cagatcgcct
     3661 catgagcaag cgcatttcga gagtttttct cgtcgacgat catccgctgg ttcgcgagtg
     3721 gctgaccacc ctgctgaacc agatcacgga tctgcgagtc tgcggcgagg cggccggtga
     3781 ggaaaaggcg atcgagggaa tccgcgccac caagcccgac
//
LOCUS       DASZMC010000476         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_191658,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000476 DASZMC010000000
VERSION     DASZMC010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191658"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VG274_09825"
     CDS             complement(<1..663)
                     /locus_tag="VG274_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE06996.1"
                     /translation="MASFSGRIALVTGASRGIGFAAAKALAAEGAHVILVARTVGGLE
                     EADDEIRTSGGTATLVPLDLKDFAAIDRLGASIFERWGRLDALLGNAGVLGTLTPLAH
                     LDPKTFAECLDVNVTANWRLIRSLDPLLRKSDAGRALFITSGASHRFRSYWGAYAMSK
                     AALEAIVLTYAAECAGTSVAANLLNPGPLRTRMRQKAMPGEDPATLDPPEAVCPLILE
                     LLS"
     gene            complement(771..2219)
                     /gene="purF"
                     /locus_tag="VG274_09830"
     CDS             complement(771..2219)
                     /gene="purF"
                     /locus_tag="VG274_09830"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWE06997.1"
                     /translation="MSTPFDDKFREECAVFGVFGHADAGALTVLGLHALQHRGQEAAG
                     IVTYDGMQFIAERHPGLVGDIFGKNSAHAQKLKGEQAVGHTRYSTTGGSGARNIQPMF
                     ADLAGGGIALAHNGNLTNARALRSDLVRQGAIFQSTSDTETIIHLMARSLYNPVVDRL
                     VDALKQVEGAYSLVALTSKKLIGARDPFGVRPLVLGQLDGAPILASETCALDIIGADF
                     VRDVERGEVVVATKDGIESYHPFSQQPDRFCIFEYVYFSRPDSMVEGRNVYEARKRIG
                     EELARESPVAADMVIPVPDSGVPAALGYAAESGIPFELGIIRNHYVGRTFIEPSERIR
                     HLGVRLKHNANGAMLKGKRVVLVDDSIVRGTTSKKIVEMVRDAGAAEVHIRIASPPTT
                     NSCFYGVDTPNREELLAHSMTVEDMRQHIHADSLAFVSIDGLYRATGESARNAKRPQF
                     CDACFSGDYPIELTDVMGGRKDVQLSLLAEVA"
     gene            complement(2216..2848)
                     /locus_tag="VG274_09835"
     CDS             complement(2216..2848)
                     /locus_tag="VG274_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWE06998.1"
                     /translation="MHALNVSWVDLAVVGVVVISTILAVVRGFVRETLSIFSWAAAAL
                     ATLYFGPAATALLRAHISTPLAAPLIAYIGIFLVVLIALSLVSHLLSEAVRRSRIGAL
                     DRSLGVPFGILRGLALVGFAYLAFSLLIPVRSQPPWLAHARFLPLIQGSSDALLALLP
                     AQYRATATGSTTTSADRAPAAGRGVRVAHAIHDLQKRLTEATGNSGNEKP"
     gene            complement(2862..>4064)
                     /gene="radA"
                     /locus_tag="VG274_09840"
     CDS             complement(2862..>4064)
                     /gene="radA"
                     /locus_tag="VG274_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWE06999.1"
                     /translation="SGEERSTRRHLTGIAEFDRVCGGGLVPGSALLVGGDPGVGKSTL
                     ILQALASFARSGGRAVYISGEEAMGQVRLRAARMGLADAPVEMGAATCVEDILATLEA
                     RPTPGLVAIDSIQTIWTSALDAAPGTIAQVRTASFDLVRYAKNTGAAMLLVGHVTKDG
                     QIAGPKAVEHLVDAVLYFEGERGHHFRVLRAVKNRFGATDEIGVFEMTGMGLMEVANP
                     SALFLGDRNSNAPGAAVFAGLEGTRPLLVEVQALVAQTSYGTPRRAVVGWDSGRLAMI
                     LAVLDARAGLGAGGHDVYLNVAGGLEIGEPAADLAAAAALISSLSGRPLPKGSVFFGE
                     ISLSGEIRPAPQPELRLKEAAKLGFTAALVPPGLKAANSGLALREVRNVGELAQWAGV
                     RQAREPAS"
BASE COUNT          756 a         1309 c         1338 g          661 t
ORIGIN      
        1 cgacagaagt tcgagaatga gcggacacac cgcttcgggt ggatcgagtg tggccggatc
       61 ctcgcccggc atcgccttct ggcgcatgcg ggttcgaagc ggtcccggat tgaggagatt
      121 cgccgccacg gatgtccccg cgcattccgc agcgtaggtg agcacaatcg cttcgagcgc
      181 ggccttgctc atcgcgtagg cgccccaata ggaccggaag cggtgcgacg cgcccgaggt
      241 gatgaagagc gcgcgccccg catcggattt tctcagcagc ggatcgagcg aacggatgag
      301 tcgccaattg gcggtgacat tgacgtcgag acattcggca aaggttttcg gatcgagatg
      361 cgcaagcggc gtcagcgtgc cgagcacgcc cgcattgccg agcagcgcat cgagccggcc
      421 ccaacgttcg aagattgacg cgcccagcct gtcgatggcg gcgaagtcct tcagatcgag
      481 cggcacaagc gtcgccgttc cgccgctcgt ccggatttcg tcgtccgctt cttccagccc
      541 gcccacggtg cgtgcgacga gaatgacatg agcgccctct gcggccagag ctttggcggc
      601 tgcgaagccg atcccgcgcg aggcgccggt gacgagtgca atacgtcccg aaaacgaagc
      661 catggtttgc tgcgcccagt gtggcgaatc acagaatcac aatgcgcatg accggctgtc
      721 atggctcgac aagctcacca tgagggcctc atcccgagcc tggcgaagga tcacgcgacc
      781 tcggcgagaa gcgagagctg gacgtctttg cggccgccca tcacgtcggt gagttcgatc
      841 ggataatcgc cggaaaaaca ggcgtcgcag aattgcggac gctttgcgtt gcgcgccgat
      901 tcgcccgtcg cgcgatagag accgtcaatc gagacgaaag cgagactgtc ggcgtggatg
      961 tgctggcgca tgtcctcgac ggtcatgctg tgggccagaa gctcttcgcg gttgggcgtg
     1021 tcgacgccgt agaagcagga attcgtggtg ggcggactcg cgatccggat atgcacttcg
     1081 gccgcgccgg cgtcgcgcac catctcgacg atcttcttcg acgtcgtgcc gcgcacgatg
     1141 gaatcgtcga ccagcacgac gcgtttgccc ttgagcatgg cgccgttcgc attgtgcttg
     1201 agacgcacgc caagatgacg gatgcgttcc gaaggttcga tgaaggtgcg ccccacatag
     1261 tggttgcgga tgatgccgag ttcgaaggga atgccggatt ccgccgcata gccgagcgcg
     1321 gcgggaacgc cggaatcggg caccggaatg accatatccg ccgcgacagg cgattcgcgc
     1381 gcaagctctt cgccgatgcg cttgcgcgcc tcgtagacat tgcgcccctc gaccatcgaa
     1441 tcgggccgcg agaaatagac atattcgaag atgcagaagc ggtccggttg ctggctgaac
     1501 ggatggtagg attcaattcc gtctttcgtg gccacgacga cctcgccgcg ctccacgtcc
     1561 cgcacgaagt cggcgccgat gatatcgagc gcgcaggttt cggaggcgag aatgggagcg
     1621 ccgtcgagct gtcccaggac gagcggccgc acgccgaacg gatcgcgcgc gccgatcagc
     1681 ttcttcgaag tcagcgcaac cagcgaatag gcgccctcca cctgtttgag cgcgtccacg
     1741 agccgatcca cgaccgggtt gtaaaggctg cgcgccatga gatggatgat cgtttccgtg
     1801 tcggaggtgg actggaaaat cgcgccctgg cgcacgaggt cggaacggag cgcgcgcgca
     1861 ttggtgaggt tgccgttgtg cgcaagcgcg attccgccgc ccgccagatc ggcaaacatt
     1921 ggctggatgt tgcgcgcgcc cgagccgccg gtcgttgagt aacgcgtatg gccgaccgcc
     1981 tgctcgcctt tcagcttctg cgcgtgcgca ctgttcttgc cgaagatgtc gcccaccagg
     2041 ccaggatggc gttcggcgat gaactgcatc ccgtcatagg tgacgattcc ggccgcttcc
     2101 tgtccgcgat gctggagcgc gtggagaccg aggacggtca gggcgccggc atcggcgtgc
     2161 ccgaacacgc cgaacaccgc gcattcctcg cgaaacttgt cgtcgaacgg ggtgctcatg
     2221 gcttctcgtt ccccgaattt cctgtggctt cggtgagcct cttctgcaga tcgtggatcg
     2281 catgggcgac gcgaactccg cgccccgcgg cgggcgcacg gtcggcagat gttgtcgtgg
     2341 atccggtcgc cgttgcgcga tattgggcgg gcagcaatgc cagcagcgcg tcgctcgatc
     2401 cctggatgag cggcaggaaa cgcgcgtgag ccagccaggg cggctgcgat cgcaccggaa
     2461 taagcaacga aaaggcgagg taggcgaacc ccacgagagc cagcccgcgc aggatgccga
     2521 acggaacgcc cagcgagcga tcgagcgcgc cgatccggga gcgtcgcact gcttcggaca
     2581 aaaggtggct caccaaggac agcgcaatca gcacaacaag aaagatgccg atataggcga
     2641 tgagcggtgc ggcaagcggg gtcgagatgt gcgcccgcag cagtgccgtg gccgccgggc
     2701 cgaaatagag cgtcgcaaga gccgctgccg cccaggagaa gatcgacagg gtttcgcgca
     2761 cgaagccgcg gacaaccgcc agaatcgtcg aaatgacgac gacgcccacg acggcgagat
     2821 cgacccagga gacattcaat gcatgcatcg attcgagtct atcatgaggc tggttcgcgc
     2881 gcttgccgca ctccggccca ttgcgccaac tcgccgacat tgcggacctc gcgcagtgcg
     2941 aggccggaat ttgccgcctt cagtccaggc ggcacaagcg cggccgtgaa gcccagcttt
     3001 gccgcctcct tgagccgcag ctccggctgc ggcgccgggc gaatttcccc cgacaggctg
     3061 atctcgccga agaaaacact tcccttcggc aggggtcggc cgctaagcga agaaatcagt
     3121 gccgcagcgg ccgcaagatc ggcggcaggc tcgccgatct ccaaaccgcc cgccacattc
     3181 agatagacat catggccgcc tgcgccaagc ccggcgcggg catcgagcac ggcgaggatc
     3241 atggcgagcc tgccgctgtc ccagccgacg acggcgcgcc gcggtgtgcc atagcttgtc
     3301 tgcgcgacca gggcctgaac ctcgaccaga aggggccgcg taccttcgag gccggcaaac
     3361 acagcggcgc ccggcgcgtt gctgttgcgg tcgcccagaa acagcgccga cgggttggcg
     3421 acttccatga ggcccattcc cgtcatctcg aatacgccga tctcgtccgt ggcgccgaag
     3481 cggtttttca ccgcgcgcag gacgcggaag tgatggcccc gttcgccctc gaaatagaga
     3541 accgcatcga cgagatgttc gaccgctttc ggacccgcaa tctggccgtc cttggtgacg
     3601 tgcccgacca gaagcatcgc cgcgccggtg ttctttgcat agcgcaccag atcgaacgag
     3661 gccgtgcgca cctgggcgat ggtccccggc gccgcgtcga gcgccgaggt ccagatcgtc
     3721 tggatggaat cgatggcgac gagaccgggc gtcggccgcg cctcgagtgt cgccagaatg
     3781 tcttcgacgc aggtcgcggc gcccatttca accggcgcgt cggcgaggcc catgcgcgcg
     3841 gcgcgcaacc ttacctgccc catcgcctct tcgccggaga tgtagacggc gcggccgccg
     3901 cttcgcgcga aggaagccag cgcctggaga ataagagtcg atttcccgac gcccggatcg
     3961 ccgcccacca gcagcgccga tcccggaacc agcccgccgc cgcacacccg gtcgaactcg
     4021 gcgatgccgg tcagatggcg gcgtgtgctg cgctcctcgc cgct
//
LOCUS       DASZMC010000477         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2947723,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000477 DASZMC010000000
VERSION     DASZMC010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2947723"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..754)
                     /locus_tag="VG274_09845"
     CDS             complement(23..754)
                     /locus_tag="VG274_09845"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE07000.1"
                     /translation="MSDIFREVEEEVRRERVEKLWKKYGDHVIALAALVVIGAAGVQL
                     YRVYEEREALKASAAFVAASQQLERGQPAAAAARFVSLAKSAPGGYAQVSRLAEADAL
                     FASGRRGDAIRIYRQIAEGSDVYLGAVSRLHAAWAIADGAPGSEVETLLAPLTDPANP
                     WHNMAREVLAYEDLRSGRTAEALKAYERIAAESDAQSPLRTRAAAMARFLNAGGDANY
                     GSVPPPKPEKSSNLSPTPSGGPPAK"
     gene            complement(851..>1651)
                     /gene="panB"
                     /locus_tag="VG274_09850"
     CDS             complement(851..>1651)
                     /gene="panB"
                     /locus_tag="VG274_09850"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWE07001.1"
                     /translation="RITVPEIRAHKNAKPVVCLTCYHAHTARLLDEHVDLMLVGDSLG
                     MVMHGMENTLGVTLDMMILHGKAVMRGSRRALIVIDMPFGSYEENPQIAFRNAARVIQ
                     ETGCSAVKLEGGARMAETIRYLSLRGIPVMGHIGLTPQMIQVLGGFKTQGRTAEEWPA
                     IEADAQAVAEAGAFAVVLEGMAEPLAAKITRQIAIPTIGIGASAECDGQILVMEDMLG
                     LNPKPAKFVRQYANMGADIEAAVKSYAAEVRTRRFPGEENIYKMKRAG"
BASE COUNT          271 a          531 c          511 g          338 t
ORIGIN      
        1 gttgcgcgca acgaattgac gttcatttcg ctggcggacc tccggagggg gtgggcgaca
       61 agttggacga tttctcaggc ttcggcggcg gcacgctgcc gtagtttgca tctccgccag
      121 cgttaagaaa tctggccatg gcggcggccc tggtgcgcag cggcgattgc gcatcggatt
      181 cggcggcgat gcgttcatac gctttcaggg cctcggctgt tctgcccgat cgcagatctt
      241 cgtaagcgag tacctcacgc gccatattgt gccaggggtt cgcgggatcc gtgagaggcg
      301 cgagcagcgt ttccacttcc gatcctggcg ccccgtccgc gattgcccag gcggcgtgaa
      361 ggcgtgagac ggcgccaaga tagacatcgc tcccctcggc gatctgcctg taaatccgta
      421 tcgcgtcgcc gcgcctgcct gaggcaaaca gcgcgtcggc ttcggcgagc ctcgagactt
      481 gcgcatatcc gccgggcgcg ctcttggcga gactgacaaa cctggctgct gcggcagcgg
      541 gctggccgcg ctccagctgt tgcgaagccg ctacaaacgc ggccgaggct ttgagcgctt
      601 cgcgctcctc gtagacgcga tagagctgca cgcctgccgc gccgatcaca accagcgcgg
      661 ccaacgcaat gacatgatcc ccatatttct tccagagctt ttcaacgcgc tcgcgacgta
      721 cctcttcctc gacttcgcga aaaatgtctg acacggagct gatccttctg gcggagcggg
      781 ccgccattcc cttctttgat ccggcgtgta tccgtggttt cgggactcga tatgcccgcg
      841 aacgcggtct ttagcccgcg cgcttcatct tgtagatatt ttcctctccg ggaaaccggc
      901 gtgtccgcac ttcggcagca taggacttca ccgccgcttc gatgtcggcg cccatattcg
      961 cgtattggcg cacgaacttc gccggcttcg ggttgaggcc cagcatgtct tccatgacca
     1021 gaatttgtcc gtcgcattcg gcggacgctc cgatgccgat cgtcgggatg gcgatctgcc
     1081 gcgtaatctt cgccgccagc ggctccgcca ttccttcgag gacgacggca aacgcgcccg
     1141 cttcggcgac ggcttgcgcg tccgcctcga tcgccggcca ttcctcggcc gtgcggccct
     1201 gcgtcttgaa tccgcccagc acctgaatca tctggggcgt gaggccgata tggcccatga
     1261 cgggaatgcc gcgaagcgac agatagcgaa tcgtttccgc catgcgcgcg ccgccttcca
     1321 gcttgacggc gctgcatccg gtttcctgga tgacgcgcgc ggcgttgcgg aacgcgatct
     1381 ggggattttc ctcgtaggat ccgaacggca tgtcgatgac gatcagagcg cgccgcgagc
     1441 cgcgcatgac ggccttgccg tgaagaatca tcatgtcgag cgtcacgccg agcgtatttt
     1501 ccatgccgtg catgaccatg ccaagcgaat cgcccaccag catcagatcg acgtgctcgt
     1561 cgagcaggcg cgcggtgtgc gcgtggtagc aggtaaggca gaccaccggc ttcgcattct
     1621 tgtgggcacg aatttcgggg acggtgatac g
//
LOCUS       DASZMC010000478         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5427654,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000478 DASZMC010000000
VERSION     DASZMC010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5427654"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..613)
                     /locus_tag="VG274_09855"
     CDS             complement(335..613)
                     /locus_tag="VG274_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07002.1"
                     /translation="MRRTATRAAIVCAILWPASAWCCGGPAGNYVAIANDGQKYTVTN
                     IGRRPVHVTFTAWSTTFDLQLAPGQSGTPYNPGVFGQMMSGYQSCYAS"
     gene            726..875
                     /locus_tag="VG274_09860"
     CDS             726..875
                     /locus_tag="VG274_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07003.1"
                     /translation="MIRKLKSGGYRLYSSKKNPKTGKRRNLGTFASRAAAEKHERAVQ
                     FFKRH"
     gene            complement(1129..1749)
                     /gene="thiE"
                     /locus_tag="VG274_09865"
     CDS             complement(1129..1749)
                     /gene="thiE"
                     /locus_tag="VG274_09865"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWE07004.1"
                     /translation="MSDRCRLYLVTPPVFDPRSFASTFKNALAAGDIASVQLRLKDVA
                     DDEIRRSADLLMPIAQTAGVAFILNDRPDLAAELGADGVHIGQDDASYREARDRVGKD
                     AIVGVTCHASRHLAIEAAEQGADYVAFGAFFPTTTKQPKARADIDLLAWWSELMSVPC
                     IAIGGITIDNARPLIAAGANFLAVCAGVWNHPDGAAAAVKDFNALF"
     gene            complement(1746..2771)
                     /locus_tag="VG274_09870"
     CDS             complement(1746..2771)
                     /locus_tag="VG274_09870"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWE07005.1"
                     /translation="MNTVELARVAHLMVTKGKGILAADESGGTIKKRFDKIGVESTET
                     NRRDYRELLFRTGDAMKNCISGVILYDETIRQNAADGTPLAKLIEESGAVPGIKVDLG
                     ARPLALCPGETITEGLDGLRERLAGYRALGAHFAKWRAVIDIGPGIPTFTAITTNAHA
                     LARYAALCQEQTLVPIVEPEVLMDGDHDIEACAVVTERVLKEVFYALYNQRVELDGMV
                     LKPNMIVPGAKCAKQASAEDVAERTIRCLKACVPASVPGIAFLSGGQSDEDATAHLNA
                     MNRRSDLPWPLTFSYGRALQAAPQKAWSGKAENVPAAQRAFAHRAHMNSLATRGEWNR
                     DREKMAA"
     gene            complement(2768..>3095)
                     /gene="pgk"
                     /locus_tag="VG274_09875"
     CDS             complement(2768..>3095)
                     /gene="pgk"
                     /locus_tag="VG274_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWE07006.1"
                     /translation="VGPDTIEQFSQRLEHARTLVWNGPLGAFETPPFERGTVAAARYA
                     AERTSAGHLLSVAGGGDTVAALNMAGVTEQFSYVSTAGGAFLEWLEGRELPGVAALMQ
                     TREKRQ"
BASE COUNT          602 a          970 c          960 g          563 t
ORIGIN      
        1 cgccgcaagt acgacgaccg caagaagaat cagcccgccc atccatgtga gtctggcggg
       61 ccggcgtgcg ggcgtccatg gtggggattg atttcattcc ccatctctat tgaccgggcg
      121 tgcaacggct gccggcggga ggattgcaac ggcccgcgca gcagcgaatt tggatgatat
      181 cagtacttgg aatcaatgac gagtgacaca aagacagatt taacgcggag gacgcggaaa
      241 aacgttggcg cgccgcaggc gcgcgattct caccctgcgc cctccgcgtt aaaaatctgc
      301 cgacagccga gtcactaaag atcaaaaatg tgcgctagct cgcgtagcag gattgatagc
      361 cggacatcat ctggccgaac acgcccggat tgtagggcgt acccgattgc ccgggcgcga
      421 gctgcagatc gaaggtggtg ctccaggccg tgaaggtgac gtgaaccggg cggcgtccaa
      481 tgttggtcac cgtgtacttt tggccgtcat tggcgattgc gacatagttg ccggccggtc
      541 cgccgcaaca ccacgccgaa gccggccaca ggatcgcgca aacgatggct gccctcgttg
      601 cggtgcgtct catgcgtcgc tcatggagtt gctccaatgt tcaaacgggt gcgcgaacgg
      661 caaggttccg gtttgcggcc ggaaccctgc gctcgggcga ccgtttcctg cgtcggagca
      721 gcaccgtgat ccgcaaactc aaatccggcg gttaccgtct ctattcgagc aagaagaacc
      781 caaaaacggg gaagcgccgc aatctcggca ccttcgcctc gcgcgccgcc gcggaaaagc
      841 acgagcgcgc cgtccagttc ttcaagcgcc actgacagcg ccggtcgggg ccgcttcccc
      901 gcaagcggca agttgcgtat gaatgcctgc gaaccgatcc agggaggtca ggatggcaaa
      961 tcgggaacag cgctccaaca gggagaaaaa gaaaccgaaa gccgacaagg acaaggtgaa
     1021 accggcagca gtacaaagct cgccattcgc agaaccgcgc agtcagaagc cgcctctgcc
     1081 gggaaagaag tagaggctgc ggcggacgag cgggtccttc gcgccggctc agaacagcgc
     1141 gttgaaatcc tttaccgccg cggcggcgcc gtcggggtga ttccagacgc cggcacagac
     1201 ggcgaggaaa tttgcgcctg ccgcaatcag cggcctcgca ttatcgatcg taattccgcc
     1261 gatggcgatg cagggcacgc tcatcagttc ggaccaccag gccagcaggt cgatgtcggc
     1321 gcgcgctttg ggctgcttcg tcgtagtggg aaagaacgcg ccaaacgcca cataatcggc
     1381 gccctgctcc gccgcctcga tcgcgagatg ccgcgacgcg tggcacgtaa caccgacaat
     1441 cgcatctttt ccgacacgat cccgtgcctc ccggtaggac gcatcatcct gtccgatatg
     1501 cacgccgtcg gcgcccagct cggctgcaag gtccggccga tcattgagaa tgaaagccac
     1561 tccggccgtc tgcgcgatag gcatgagcaa atcggcggag cgacggattt catcatccgc
     1621 aacgtccttc agccgaagct gcacggacgc gatgtcgcca gcggcgagcg cgtttttgaa
     1681 ggttgatgca aagctgcgcg ggtcgaaaac gggtggcgta acgagataaa gtcggcaacg
     1741 atcgctcacg cggccatttt ctcccgatcg cgattccatt cgccgcgcgt ggcgaggctg
     1801 ttcatatggg cgcgatgggc gaaggcgcgc tgcgccgcgg gaacattttc cgccttgccg
     1861 ctccacgcct tttgcggtgc cgcctgcagc gcacgcccgt aggagaaggt gagcggccag
     1921 ggaagatcgg aacgccggtt catcgcgttc aggtgcgccg ttgcgtcctc gtcggattgc
     1981 ccacccgaaa gaaacgcgat cccgggaacc gaagccggga cgcaagcctt caggcagcgg
     2041 atcgtgcgtt cggcgacgtc ttcggcactc gcctgcttcg cacatttcgc gcccggcacg
     2101 atcatgttgg gcttgagcac catgccgtcg agttcgacgc gctgattgta gagcgcgtaa
     2161 aaaacttcct tcagcacccg ttcggtcacc acggcgcagg cctcgatgtc atggtcgcca
     2221 tccatcagca cttcgggctc gacgatcggc acgagcgtct gctcctgaca aagcgcggca
     2281 taacgggcga gtgcgtgtgc attggtggtg attgccgtga acgtcggaat gccggggccg
     2341 atatcgatga ccgcgcgcca tttggcgaaa tgtgcgccga gcgcgcgata tccggcgagc
     2401 ctctcgcgca atccgtcgag gccttcggtg atcgtttcgc cggggcagag cgccaatggc
     2461 ctggcgccga ggtccacctt gatgcccgga accgcgcccg attcttcgat cagcttcgca
     2521 agcggcgtcc cgtcggctgc gttctgccgg atggtttcgt catagagaat gacgcccgag
     2581 atgcaattct tcatcgcatc gccggtgcga aacagaagct cgcgatagtc gcgcctgttg
     2641 gtctccgtcg actccacgcc gatcttgtcg aagcgcttct tgatggtgcc cccgctttcg
     2701 tcggccgcca gaatgccttt gcctttcgtc accatcaaat gcgcgacccg cgccagttcc
     2761 accgtgttca ttgccgtttt tccctggttt gcatgagagc cgccaccccg ggcagctctc
     2821 gtccttccag ccattcgaga aatgcgccgc cggctgtgga gacataggag aattgttccg
     2881 tcacgcccgc catgttgaga gcagccacag tgtcgccgcc acctgcaaca gatagcaggt
     2941 gaccggctga cgttcgctcc gccgcgtacc gcgcggctgc gacggtgcca cgctcgaagg
     3001 gcggggtttc gaaggcgccg agtggtccgt tccagaccag cgtccgcgcg tgttcgagcc
     3061 tttgcgagaa ttgctcgata gtatcgggtc ccaca
//
LOCUS       DASZMC010000479         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1841671,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000479 DASZMC010000000
VERSION     DASZMC010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841671"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VG274_09880"
     CDS             <1..673
                     /locus_tag="VG274_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HWE07007.1"
                     /translation="AADGTERLVHSFNVNHGGFFPFADLLINADADLYGTDIYGGPKN
                     FGTVFEIAPGGAETVLRTFKGSPSDGAEPQGGVIMDGMGNLYGTLRSGGHSGCDSDSG
                     CGAVFKLASDGTETILHFFSGKHGDGGNPVAGLTADQAGNLYGTTEYGGGSCTLEEAY
                     GCGTVFRIMPDGTEAILYFFSRQNGANGAFPMSGLVADGAGNLYGTASGGGAYGYGNV
                     FEITP"
     gene            complement(804..1817)
                     /gene="glk"
                     /locus_tag="VG274_09885"
     CDS             complement(804..1817)
                     /gene="glk"
                     /locus_tag="VG274_09885"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HWE07008.1"
                     /translation="MIPAKTATAKTFGLVADIGGTNVRFATVDLQAPQPEIDSLRFLS
                     TRGHPDIVAAAKTYLRDAELHAPPRALVFAVAGPVENNEIHLTNAGWRISAEELREGL
                     GAPYARLVNDFEAVALAVPHLQEHDLAAIGPSPKFDPHGDGTIAIVGPGTGLGVAGLV
                     NAHDAHIALVTEGGHAALAPTDDLEMRLVEILMRKFGRVSAERILSGPGLLNLYGAMA
                     EIDGFTPDERTPEGITQIARGDPSSFEAKIFARFCAILGSVAGDIALTMGARQGVLIA
                     GGILPDAAELFAASRFRERFEDKARFRDYMRAIPTSLILQPHAGLIGAASILLQACGK
                     ASR"
     gene            complement(1814..>2766)
                     /gene="zwf"
                     /locus_tag="VG274_09890"
     CDS             complement(1814..>2766)
                     /gene="zwf"
                     /locus_tag="VG274_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWE07009.1"
                     /translation="FRIDHYLGKETVQNLLALRFANVLFEPLWNRACIDHVQITVAES
                     VGVEGRWAYYDAYGALRDMVQNHILQLLCLVAMEPPARLDPDAVRDEKAKVLRSLRPI
                     RGREAAAKTARGQYVQGQIEGVPVPGYESEGSESNTETFVAIEAEIENWRWAGVPFYL
                     RTGKRLAQRHTEIVIQFLGVPHSPFGRAGNRDLIPNRLTITLQPQERISLLLMNKTPG
                     LTADGMRLGPLPLDLSLTDAFRAQRRRIAYESLLLDALTGNPTLFVRGDEVEAAWAWI
                     DGIAAGWKEAGIAPEFYAAGSRGPEAADALIARRGYSWSA"
BASE COUNT          524 a          838 c          873 g          531 t
ORIGIN      
        1 cgctgcggac ggcacggaac gactggtcca ttccttcaac gtcaatcatg gcggattttt
       61 tccattcgcc gatctgctca tcaacgcaga tgccgatctg tacggcactg atatctacgg
      121 cggtccgaag aattttggta ctgtcttcga gattgcacct ggaggtgctg aaacagtgct
      181 ccgcacgttc aaaggttcgc cgagtgatgg tgccgagcct cagggtggcg tgatcatgga
      241 tgggatgggt aatctctatg ggacgttgag aagtggcggc cattcgggct gtgactcaga
      301 ttcaggctgc ggcgcggtgt tcaagttggc atcggacggc acggagacga ttctccattt
      361 cttctcaggc aagcatggag acgggggtaa tccggtcgct ggactgactg ccgatcaagc
      421 cggcaatctc tatggcacca ctgaatacgg cggcggcagt tgcactctcg aggaagccta
      481 tggctgcggc actgtcttca ggattatgcc agatggcaca gaagcgattc tttatttctt
      541 ctccaggcag aatggcgcga acggcgcctt tccgatgtcg gggcttgtcg cagacggtgc
      601 gggcaatctt tacggaacgg cgagcggcgg aggcgcctac ggctatggca acgtgttcga
      661 gatcacgccc tgaatggtgg gcaaatcgct ggcaacgaag ggcaatgatg gcgtccggac
      721 gaacggtgcc tcctggtcgt atttcgccaa gggcctcgac ctgacggggg gccggccaag
      781 tcttggcgaa gatcaggcag gcttcatcgg ctcgctttgc cgcaagcctg caacaggatc
      841 gacgcggctc cgatcagccc cgcatggggc tgcagaatga gcgaggtcgg aatcgctctc
      901 atgtaatccc tgaaccgtgc cttgtcctca aagcgctcgc ggaacctgct cgcggcaaag
      961 agttcggccg cgtccggcag tatcccgccc gcgatgagga cgccctggcg cgcgcccatg
     1021 gtgagcgcga tgtcgccggc gaccgagccc aatatcgcgc agaaccgcgc gaatatcttt
     1081 gcttcgaatg acgaaggatc gcctcgcgca atctgcgtga tgccttcggg cgtgcgttca
     1141 tccggggtga aaccgtcgat ttccgccatc gccccgtaaa gattgagcaa gcctggaccc
     1201 gagagaatgc gctcggccga gacgcgcccg aatttccgca tcaggatctc aacgagccgc
     1261 atttccaaat cgtcggtcgg cgcaagggcc gcgtggccgc cctccgtcac gagcgcaatg
     1321 tgggcatcat gggcattgac caatccggca acgcccaatc cggtgcccgg accgacgatt
     1381 gcaattgttc catcgccgtg cggatcgaat ttgggcgatg gaccgatcgc cgcgagatcg
     1441 tgctcctgca aatgaggaac ggccagggcg accgcctcga aatcattcac cagcctggcg
     1501 taaggggcgc ccagcccctc tcgcaattcc tccgcggaaa tgcgccagcc cgcatttgtc
     1561 agatggattt cgttgttttc gaccgggccc gccacagcga agacgagcgc cctgggcggc
     1621 gcgtgaagtt ccgcgtcgcg aagataggtc tttgcagcgg caacaatgtc gggatgtccg
     1681 cgcgtggaca agaagcgcag cgaatcgatt tccggttgcg gcgcttgcag atcgaccgtg
     1741 gcgaagcgga cattggttcc gccgatgtct gcgacgagac cgaacgtctt cgccgtcgcg
     1801 gtcttcgccg ggatcatgcg gaccagctat agccgcgccg ggcgatcagc gcatcggccg
     1861 cttccggccc gcgcgaacca gccgcgtaga attccggtgc gatcccggcc tccttccagc
     1921 ccgcggcgat accatcgatc caggcccacg ccgcttccac ctcgtcgccg cgcacgaaaa
     1981 gcgtcggatt gccggtcagc gcatcgagaa gcagactctc ataggcgatg cggcggcgct
     2041 gggcgcggaa cgcatcggta aggctcagat caagcgggag cggaccgaga cgcatgccgt
     2101 cggccgtcaa tcccggtgtt ttgttcatca gcagaagaga tatccgttcc tgcggctgga
     2161 gcgtgatcgt gagccggttc ggaatcagat ccctgttgcc cgcgcggccg aatggcgagt
     2221 gcggcacgcc aagaaactgg atcacgattt cggtgtgccg ctgcgcgagc cgcttgccgg
     2281 ttcgcaaata gaacggcacg cctgcccagc gccaattctc gatttcggcc tcgatcgcca
     2341 cgaaggtttc cgtgttgctc tcgctgccct ccgactcata accaggaacg gggacgccct
     2401 cgatttgtcc ttgcacatat tgcccgcgcg cggtttttgc ggcggcttcg cgtccgcgga
     2461 tcggcctgag cgagcgcaga accttcgcct tctcgtcgcg gaccgcatcg ggatcaagcc
     2521 tggccggcgg ctccatggcg acaaggcaga gaagctgcag gatgtgattc tgcaccatgt
     2581 cgcgaagcgc gccataggcg tcgtaatagg cccagcgccc ctccacgccg acggattcgg
     2641 cgacggtaat ctggacgtga tcgatgcagg ctctgttcca caagggttcg aacagcacgt
     2701 tggcgaagcg cagcgcgagc aggttctgga ccgtttcctt gccgagataa tggtcgatgc
     2761 ggaaga
//
LOCUS       DASZMC010000480         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5428012,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000480 DASZMC010000000
VERSION     DASZMC010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5428012"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VG274_09895"
     CDS             <1..1307
                     /locus_tag="VG274_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWE07010.1"
                     /translation="TAVVVSNVAVGIWFGATQEQITEASITERLLDRAVSAATLLSGI
                     PAREREAAVQTMSSGPWRFRLVYGKPVIRPMTDEEARYAARVRAMLPPERARQPIAVS
                     FRSGVLPPAAGVPNAVARSGTIVEVTLPVVRRTELVTSFSRPGSPSWPLEVGIAGLVA
                     MVTTSMGAALVARRIARPISQLAAAATEAARGGAAPRVPEEGPDDVRRAAHAFNLMTD
                     QVSRTLESQRQLLSAVGHDLRTPITAMRINSEFIEDVELRERMQKSLEELQELTEAVL
                     SAGRGAGGEKSRKIDLAALIESLCTDMDEMGEPVSWSTHAAAPITCRPNEIRRAVRNL
                     IQNAVAYGSRADVCLDAKPDRYDIIVEDDGPGIAETDRKRVFEPFVRLEASRSTETGG
                     SGLGLTLVKAIAEGHGGHITLESRSERGLRARLSLPLAPADA"
     gene            1316..1942
                     /locus_tag="VG274_09900"
     CDS             1316..1942
                     /locus_tag="VG274_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWE07011.1"
                     /translation="MQFTRNSPARTPRIGAIVLAAGTSSRMGRNKLLIEIDGEPLVRH
                     VAATVAASRASPVVVVTGSEEENMRAALTGLPVSIVFNPDYREGLSASLRTGVQEIPD
                     GCDGAVVVLGDMPGVSGTLVDRLIDAFDPDENREICVATSGGKRGNPVLWGRRFFADL
                     LGIAGDVGAKHLIAENEEMVCEVEAPDEDPLTDIDTPEALAAWLARHK"
     gene            1939..2388
                     /locus_tag="VG274_09905"
     CDS             1939..2388
                     /locus_tag="VG274_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWE07012.1"
                     /translation="MSDTLYTMDLLRLAADAHGAGKLAVSSASGHAFNPACGDKVAVD
                     LLLAGGKILEMAHETKACVLAQASSSILGQALRGADQAAVERLHASVEAMLISTAAPP
                     PVPFERYAAFQGAVEYPSRHRCVLLPIQAVLDALDRSSRATCNEMNP"
     gene            2538..>2772
                     /locus_tag="VG274_09910"
     CDS             2538..>2772
                     /locus_tag="VG274_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07013.1"
                     /translation="MRMGAGPFTYQTFEDDDPRVLYHDVVVALDEDNEINNGQPSLWA
                     RVFDELNTQPGERILHIGCGSGYYSAILAEIAGT"
BASE COUNT          510 a          883 c          899 g          480 t
ORIGIN      
        1 ccacggcggt ggtcgtctcc aacgtcgccg tcggtatctg gtttggtgcg acacaggagc
       61 agattaccga agcttcaatc acggagcggc tgctcgaccg cgctgtttcc gccgcgaccc
      121 tgttgagcgg cattcccgcg cgcgaacgcg aagcagccgt gcagaccatg agttcagggc
      181 catggcggtt tcgtctcgtt tacggaaagc ccgtgatccg cccgatgacc gacgaggagg
      241 cccggtatgc agcgagagtg agggccatgt tgccgcccga acgcgccagg caaccgatcg
      301 ccgtgagctt tcgttcgggc gtcctgcctc ccgcggcggg cgtgccgaat gccgtggcga
      361 ggagcggcac gatcgtcgag gtgacgttgc ctgtggtgcg gcgcaccgaa ctcgtcacca
      421 gtttctcacg ccccggctcg ccctcgtggc ccctggaagt cggcatcgcc gggctggtgg
      481 cgatggtcac gacgtcgatg ggcgcagccc ttgtcgcccg caggattgcg cgccccatct
      541 cgcagctggc tgcggcagcg accgaagcgg cccgcggggg cgccgctccc cgcgtgccgg
      601 aagaagggcc cgacgatgtg cggcgcgccg cgcacgcgtt caatctgatg acggatcaag
      661 tgtcgcgcac gctggaaagc cagcgtcagc ttctttcggc ggtcggccac gatctgcgca
      721 cgccgattac cgcgatgcgc atcaattcgg aattcatcga agatgtggaa ttgcgcgagc
      781 gcatgcagaa aagtcttgaa gagttgcagg aattgaccga agcggtcctc tccgccggtc
      841 gcggggccgg cggcgaaaag tcccgaaaga tcgatctcgc tgcgctgatc gagagcctgt
      901 gcaccgacat ggacgagatg ggcgaaccgg tttcctggtc gacgcatgcc gccgccccca
      961 tcacctgccg gccgaatgag attcgccgcg cggttcgcaa cctgattcag aatgccgtcg
     1021 cctatggcag ccgcgcggac gtctgcctgg acgcgaaacc ggatcgctac gacatcatcg
     1081 tcgaggatga tggccccgga atagcggaaa ccgaccgcaa gcgcgtcttc gaaccgttcg
     1141 tgcggctcga agcctcgcgc agcaccgaga cgggcggctc gggtctcggc ctgacgctgg
     1201 tcaaggccat cgccgaaggc catggcggcc acatcaccct cgagagccgc tccgaacgcg
     1261 gcctgcgcgc ccggctcagt ttgcccctcg cgcccgcgga cgcctaatct cgcgcatgca
     1321 attcacacgg aactcgcccg cgcgcacacc caggatcggc gcaatcgttc tggcggctgg
     1381 cacgtcgtcg cgcatgggac gaaacaagct gctgatcgag atcgatggcg aaccccttgt
     1441 gcgccacgtt gcggcgacgg tcgcggccag tcgcgcgagc cccgtcgtcg tcgtaaccgg
     1501 cagcgaagag gagaacatgc gcgcggccct gacaggcctt cccgtgtcga tcgtcttcaa
     1561 tcccgattac cgcgaaggtt tgagcgcgtc gctgcgaacc ggtgttcagg aaatcccgga
     1621 tggctgcgat ggcgcggtgg tggtgctggg cgacatgccc ggcgtatcgg gcacgctggt
     1681 cgacagattg atcgacgcgt tcgaccccga cgagaatcgc gaaatctgtg ttgccacgag
     1741 tggaggcaag cggggcaacc cggtgctctg gggtcgccgc tttttcgcag acctgctcgg
     1801 cattgccggc gatgtgggcg cgaagcatct gattgccgag aatgaggaga tggtctgcga
     1861 agtcgaagcg cctgacgagg acccgctcac cgacatcgat acgcccgagg cgctcgcagc
     1921 ctggctcgcc cgccacaaat gagcgatacg ctttacacca tggatctcct gcggcttgcg
     1981 gccgacgctc acggcgcagg aaagcttgcg gtgtccagcg cctccggtca tgccttcaat
     2041 cccgcatgcg gagataaagt ggcggtcgac cttcttctcg cagggggaaa gattctcgag
     2101 atggcgcacg agacgaaagc ctgcgtactc gcccaggctt cgtcgtccat tctgggccag
     2161 gcgctcagag gtgccgatca ggcggcagtg gaacgattgc acgcaagcgt ggaagcgatg
     2221 ctcatatcca cggccgcacc tccgccagtc ccttttgagc gttacgcggc ctttcaaggc
     2281 gccgtcgaat accccagccg tcaccgctgt gtgctcttgc ccatccaggc ggtactcgac
     2341 gcgctcgacc gaagttcccg cgcgacatgc aacgagatga acccgtgacg aactcgatcg
     2401 aagaggcacg aatatggttt gcggaagatt tgcgcgtggc cgcttccgtc gggtcaagac
     2461 cagtaatcga cgcattcgcc cgggtgccac gcgaaaggtt catcgggccg ccaccatggc
     2521 gtatcggcac gcgactgatg aggatgggtg cgggaccgtt cacgtatcaa acatttgagg
     2581 acgatgatcc tcgcgtgctc taccatgatg tcgtcgtcgc gctcgatgag gacaatgaaa
     2641 tcaacaacgg ccaaccgagt ctgtgggcca gggttttcga cgagctgaac acccagcccg
     2701 gggaacggat cctgcacata ggctgcggca gcggctatta ctcggcaatt ctggccgaaa
     2761 tagcgggtac aa
//
LOCUS       DASZMC010000481         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5141988,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000481 DASZMC010000000
VERSION     DASZMC010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5141988"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VG274_09915"
     CDS             complement(<1..1376)
                     /locus_tag="VG274_09915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWE07014.1"
                     /translation="MKSVLARFLRALPLLLLAPFLLFIAALALALADFVALFTPPIPP
                     TSRKPSSRAASLVIPNWNGRHLLENFLPSWLAAIEAHPGSEVLIVDNGSTDGSAEWIR
                     SHYPQARVLALERNLGFGGGSNAGFREASNDVVVLLNSDMRVEPDFLAPLLAGFAPHG
                     KGEDESVFAVSCQIFLGDPSKRREETGLTEGWWQDGGLRVSHREDPAVDRLFPCFYGG
                     GGSCAFDRAKFLELGGFDELLAPFYLEDTDIGFQAWKRGWKVLYQPASVVHHEHRGTI
                     GRKFSASYIQSVLRKNYALFCWKNIHEWRRLASHLLHSFAGSVVTAFAGDSPTRASAA
                     GIARAFLQLPAAVRSRIRARRLAVISDSEAFRRSRPLYFHDRFSPFHPHPARPRVLFV
                     SPYPICPPTHGGGLFMFNTIRELSRNADVHAIVMLDWPEERAANEELRRWCKSLELLV
                     RTADRAPHL"
     gene            complement(1373..2515)
                     /locus_tag="VG274_09920"
     CDS             complement(1373..2515)
                     /locus_tag="VG274_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWE07015.1"
                     /translation="MHEFTGERVIPGLVDADLFNEHLSRYRFAARFAAPEMAVLDAGC
                     GAGYGSAEFNARAVAAIDLSPEAVVHAATHYARPGAFFAAASCAALPFRDAAFDLVVS
                     FEVIEHLPDYRALLSEARRVLKSGGVFLVSTPNRAYYAESRAQAGPNPFHVREFDYAE
                     FTQILAGFFPHVEIWTQNHTDAIVFSPASPAPISTKAPALESSASADPQTAHFFLAAC
                     SSQPIASRPLFAWLPSAANTLRERELHIARLAAELATKDAWLNDALARHESLHRAHQE
                     LLSELERRSQWAGHLNHEIAQRDARIAELQQEAAVRLTWVRDLEDQIARGGAQIETLN
                     AELIERAKWAQSLEAEIESLSHTRWMRLGKKLHALPHAESPGEHAE"
     gene            complement(2508..3791)
                     /locus_tag="VG274_09925"
     CDS             complement(2508..3791)
                     /locus_tag="VG274_09925"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE07016.1"
                     /translation="MTAVEFEHVSKRYAIYDSPADRLKEFATLNRRRFHRDFEALRDV
                     SFEIRQGEIFCIVGENGSGKSTSLQLMAGILQPSAGAVRVNGRVAALLELGAGFNPEF
                     TGRENARLYCSILGLTARDIAQRSAAIEAFAEIGAFIDQPIRTYSSGMLVRLAFACAI
                     HTGPEILIVDEALSVGDAYFRQKCMRRIHELRAGGVTIVFVSHAIAEVKAIGERVLWL
                     DRGRVAAVGDPDAVISAYAGAMSERDRRYESATHPRAARDETEVVRGIPNIDARQGDG
                     SAEILGIEVRDAFGDPLHLMTPESSIAVRISARAIADIARPDLGFTLRNHLGVEFARV
                     TLAQEGRRLAPLRPGDTVTACFRVRIPELYPGAFSFSPFISGGSPANPAFCDLVENAI
                     TIPMARGERIVYGYLHVPCRVERAPTPQPAERSLA"
     gene            3895..4143
                     /locus_tag="VG274_09930"
     CDS             3895..4143
                     /locus_tag="VG274_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWE07017.1"
                     /translation="MTVSVTEAKNKLTQLLTAVENGERVTIERHGHIIAEIVKPSGKK
                     IPKFGTLKGIVHMTPEQFHEATRPMTDEEVDAFLEGRY"
     gene            4144..4527
                     /locus_tag="VG274_09935"
     CDS             4144..4527
                     /locus_tag="VG274_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE07018.1"
                     /translation="MRVLLDTTTLYAAAGVSAVSFTPKVRRLLEDPDTVRLVSPVSFN
                     EIAIKANKGLTPLTREHIRKLISDLALTVLPLTAEHSLRLFGLPAHHGDPFDRMLIAT
                     ALAEDVAIVASDREFKRYKGVRVIW"
BASE COUNT          891 a         1202 c         1836 g          785 t
ORIGIN      
        1 agatgcggcg cgcggtcggc ggtccggaca aggagttcga gcgatttgca ccagcgccgg
       61 agttcttcgt tggcggcgcg ttcttccggc cagtcgagca tcacgatggc atggacatcg
      121 gcgttacgcg agagttcgcg gatggtgttg aacatgaaca atccgccgcc gtgcgtgggc
      181 ggacagatgg gatagggcga gacgaagaga acgcggggac gcgcggggtg cgggtggaaa
      241 ggcgagaagc ggtcgtgaaa gtagagcggc cgggagcggc ggaaggcttc ggaatcgctg
      301 atgacggcga ggcggcgggc gcggatgcgg gagcggacag cggcggggag ttgcaggaag
      361 gcgcgggcga tgccggcggc gctcgcgcgg gtgggggaat cgcctgcaaa cgcggtgacg
      421 acggatccgg cgaacgaatg aagcaagtgt gacgcgaggc ggcgccattc gtggatgttt
      481 ttccagcaga agagcgcgta gttcttgcgc aggacggact gaatatagga cgcgctgaat
      541 tttcggccta tggtgccgcg gtgctcgtga tgcacgacgc tggccggctg atagaggact
      601 ttccagccgc gcttccaggc ctgaaagcca atatcggtgt cttcgaggta gaaaggcgcg
      661 agcaactcgt cgaaaccgcc gagttccaga aatttggcgc gatcgaaggc gcaggagccg
      721 ccgccgccgt agaagcaggg gaaaagtcga tcgacagccg gatcttcgcg atggctgacg
      781 cgcaggccgc cgtcctgcca ccagccttca gtgaggccgg tctcctcgcg acgtttggac
      841 ggatcgccga ggaagatctg gcaggagacg gcgaagacgg attcgtcttc tcctttccca
      901 tgcggggcga agccggcgag gaggggcgcg aggaaatcgg gctcgacgcg catgtcgctg
      961 ttgaggagaa cgacgacgtc attcgaagcc tcgcggaaac cggcattgga gccgccgccg
     1021 aagccgaggt tacgttcgag cgcaagcacg cgggcttgcg gataatggct gcgaatccat
     1081 tcagcgctgc catcggtgga gccgttgtcg acaatcagga cctcgctgcc cgggtgggct
     1141 tcgatggcgg cgagccagga ggggagaaag ttttcgagga gatggcggcc gttccagttg
     1201 gggataacga gggaggcggc acggctggag ggtttgcggc tggtgggcgg gataggcgga
     1261 gtaaagagcg cgacgaaatc cgcgagggca agagcgagcg ccgcgataaa gaggagaaag
     1321 ggcgcgagga gcagaagcgg cagggcgcgg agaaagcgcg cgaggacgga cttcattcgg
     1381 cgtgttcgcc ggggctctcg gcatgcggaa gagcgtgcag ctttttgccg agccgcatcc
     1441 agcgggtatg tgagaggctt tcgatttcgg cttcgagaga ttgcgcccat ttggcgcgct
     1501 cgatgagttc ggcgttcagg gtttcgatct gggcgccgcc gcgggcgatc tggtcttcga
     1561 gatcgcgaac ccaggtgagg cgaacagcgg cctcctgctg gagctcggcg atgcgggcgt
     1621 cgcgttgcgc gatttcatgg ttgagatgcc cggcccactg gctgcggcgc tcgagttccg
     1681 agaggagttc ctggtgtgcg cgatggagtg attcgtggcg cgcgagagcg tcattgagcc
     1741 aggcatcttt ggtggcgagt tcggcggcga ggcgggcgat gtggagttcg cgctcgcgca
     1801 gagtgttggc ggcggagggg agccaggcga agagagggcg cgaggcgatg ggctggctgc
     1861 tgcaggcggc gaggaagaag tgggcggttt ggggatccgc ggaggcggac gactcgaggg
     1921 ccggggcttt ggttgagatg ggcgccggcg aggcgggcga aaagacaatg gcgtcggtat
     1981 ggttctgggt ccagatttcg acgtggggga agaagccggc gaggatttgg gtgaattcgg
     2041 cgtaatcgaa ctcgcggacg tggaacggat tggggccagc ctgggcgcgg gattcggcat
     2101 aatacgcgcg gttgggggtg gagacgagaa agacgccgcc ggatttcagc acgcggcgcg
     2161 cttccgagag cagcgcgcgg taatcgggca ggtgctcgat gacttcgaag gagacgacga
     2221 gatcgaaggc ggcgtcgcgg aacgggaggg cggcgcagct ggcggcggcg aagaaggcgc
     2281 cggggcgggc gtaatgggtg gcggcgtgga cgacggcttc gggcgagagg tcgatggcgg
     2341 cgacagcgcg cgcgttgaat tcggcgctgc cgtagccggc gccgcagccg gcgtcgagga
     2401 cggccatttc gggcgctgcg aaacgagcgg cgaagcggta gcgcgagagg tgctcgttga
     2461 acagatcggc gtcgacgagg ccggggatga cccgctcgcc ggtgaattca tgcaagcgaa
     2521 cgctccgcgg gttgaggggt tggcgcccgc tccacgcggc aggggacgtg gaggtagccg
     2581 tagacgatgc gctcgccgcg cgccatggga atggtgatgg cgttctcgac gagatcgcag
     2641 aaggcggggt ttgcgggcga gccgccggag ataaatggcg agaaggagaa cgcgccggga
     2701 tagagctcgg ggatgcggac gcggaagcac gcggtgacgg tgtcgccggg gcgcaggggc
     2761 gcgaggcggc ggccctcctg ggcaagggtg acgcgcgcga attcgacgcc gaggtgattg
     2821 cgcagggtga agccgagatc ggggcgggcg atatcggcga tggcgcgggc gctgatgcgg
     2881 acggcgatgg aggattcggg cgtcatgagg tgaagcgggt cgccgaacgc gtccctgact
     2941 tcgatgccga gaatttcggc gctgccatcg ccctgacgcg cgtcgatatt ggggatgccg
     3001 cggacgactt cggtttcgtc tcgcgcggcg cgcgggtggg tggcggattc gtagcggcga
     3061 tcgcgctcgg acatggcgcc ggcgtaggcg gagatgacgg catcgggatc gccgacggcg
     3121 gcgacgcggc cgcgatcgag ccagaggacg cgctcgccga tggctttcac ttcggcgatg
     3181 gcgtgggaga cgaagacgat ggtgacgccg ccggcgcgga gctcgtgaat gcgacgcatg
     3241 cacttctggc ggaagtaggc gtcgccgacg gagagggctt catcgacgat gaggatttcg
     3301 gggccggtgt ggatggcgca ggcgaaggcg aggcggacga gcatgccgct cgagtaggtg
     3361 cggatgggtt gatcgatgaa ggcgccgatt tcggcgaagg cttcgatggc ggcggagcgc
     3421 tgggcgatgt cgcgcgcggt gaggccgagg atggagcagt agaggcgggc gttttcgcgg
     3481 ccggtgaatt cggggttgaa gccggcgccc aattcaagca aagccgccac gcggccgttg
     3541 acgcggacgg cgcccgcgct gggctggagg atgccggcca tgagctggag cgaagtgctt
     3601 ttgcctgagc cgttttcgcc cacgatgcag aagatttcgc cctggcggat ttcgaagctg
     3661 acgtcgcgca gggcttcgaa atcgcgatgg aaacggcgcc ggttgagagt ggcgaattcc
     3721 ttcaggcgat cggcgggcga atcgtaaatg gcgtagcgct tcgagacgtg ctcgaattcg
     3781 acggcggtca cgaacttttt agtttaatcg cgcacaactg accagaggtt ttggcccgcg
     3841 ggcgtccgct ggcggatatt tgcaaattgg ctagccaata gccacaatat agatatgacc
     3901 gtcagcgtta cggaagcgaa gaacaagttg actcagcttt tgacggcggt cgagaacggc
     3961 gagcgcgtga ccattgaacg tcacggccac atcattgccg aaatcgtgaa gccgtccggg
     4021 aaaaagattc cgaagttcgg aacgctgaaa ggcattgtac acatgacgcc tgagcagttt
     4081 cacgaggcga cgcggccgat gacggacgaa gaggtcgacg cgttcctcga agggcgctat
     4141 tgattgagag ttcttctcga cacaaccacg ctgtatgcgg cggcgggtgt ttccgcggta
     4201 tcgttcaccc cgaaggttcg gcgcctgctg gaggatccgg acacggtgcg tctggtcagt
     4261 ccggtctctt ttaacgaaat cgcgatcaag gcgaacaagg gactcacccc attgacgcgg
     4321 gaacacatca ggaagctcat ctctgacctg gccttgaccg ttttgccatt aacagccgag
     4381 cattccctca ggctgtttgg actgcccgct caccatggcg atccgtttga ccgaatgctg
     4441 attgcgacgg cgctggcgga agacgtcgcc atcgtggcca gcgatcgcga attcaagagg
     4501 tacaaaggag tccgggtgat ctggtgattt cacgcgaaat ccactcgtta gcgcgcgggc
     4561 gcgggttgtc tggcaatcga tgtccggagt gcttccagga ttatcgacca ggctgacagc
     4621 agaagaagca tggcggcgag ccatccggca gcattgtaaa ctcgaagcgc gaccgggcga
     4681 acggcctgaa ctttgaacgg attggcgcgc gagt
//
LOCUS       DASZMC010000482         8265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3500537,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000482 DASZMC010000000
VERSION     DASZMC010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3500537"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1152)
                     /locus_tag="VG274_09940"
     CDS             complement(316..1152)
                     /locus_tag="VG274_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWE07019.1"
                     /translation="MTDRLTVTILGCGSSGGVPRIGGPDGKGEWGACDPANPKNRRRR
                     CSILVRRHGAQGVTTLLVDTSPDMREQLLGARTAWLDGVIITHDHADQLHGIDDLRTV
                     VHAMRRRVDVYSDRTTLDTVEQRFGYCFSTPPGSFYPPILNSHVLAEPFVEFAIEGAG
                     GPIPVLPFWQEHGSIRSLGLRCGPIAYSSDVNGLPEDAFMALEGVACWIVDALRYTPH
                     PTHANVAAALAWIARLKPAHAVLTNLHFDLDYETLRRELPEGVEPAYDGMVVTAALPH
                     TC"
     gene            complement(1149..1931)
                     /locus_tag="VG274_09945"
     CDS             complement(1149..1931)
                     /locus_tag="VG274_09945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWE07020.1"
                     /translation="MLVDSHCHLDFPEFAAELPDVVARAKAAGVGVCVTIGTRLSGFA
                     AVAAVAAHFPDIYCSVGIHPHEAEKEPLDEAAPLLALARNPKVVGIGETGLDYYYEHG
                     PRPRQIANFRAHITAARTLALPLIVHTRDAEDDTIAILREEMGRGAFAGLIHCFTGTQ
                     RLADAALDLGLCISASGIATFKKSDTLRAVLERVPLDRLLVETDSPYLAPTPHRGKRN
                     EPAFVVHTAAMLAGLRGVAVDELARATTDNFFRLFTRAARPA"
     gene            complement(1947..2996)
                     /locus_tag="VG274_09950"
     CDS             complement(1947..2996)
                     /locus_tag="VG274_09950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HWE07021.1"
                     /translation="MAKRARDEETIETDRIAGVPHPRETTTLIGQDEALARAARAIRG
                     GRPPQAWLIGGPPGVGKATFAYRVARYLLRYGATDAGPEDLSLPANDPVAALVRAGAH
                     PGLLAIRRGPHPDTGKPMTVLSVDEIRKLGNFFGLTSASGGWRIAVIDTADEMNEQAA
                     NALLKILEEPPRRAMLMLLAHAPARLASTIRSRCQALHLQPLSEASLAAALAERLPQV
                     SAADGARLVALSGGSIGAALRLVGEDGLKLAAEAEKLIERAAAPDFAATLALAERVAK
                     FDRGMETFGVYLTHMLAARILARAREGAPHLDRWVELCNRIRASFIRSAALHLDPRQT
                     IVSAAQAIASRRGTL"
     gene            complement(2996..3628)
                     /gene="tmk"
                     /locus_tag="VG274_09955"
     CDS             complement(2996..3628)
                     /gene="tmk"
                     /locus_tag="VG274_09955"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWE07022.1"
                     /translation="MAAPARFITLEGGEGAGKSTQAKRLAAALEARGIAVCATREPGG
                     SPGAEEIRRLLVDGAPGRWDALSETLLLFAARADHLAHTIRPALAAGRCVVCDRFTDS
                     TYAYQGAGRGLNEATISAIEAAAIGSLRPDLTLILDIAPAAGLARVDSRGRAETRFEQ
                     FDAGFHARLREWFRALAQSEPDRCVLVDACAPVDEVTAQIWQTVSARFGL"
     gene            complement(3628..4830)
                     /locus_tag="VG274_09960"
     CDS             complement(3628..4830)
                     /locus_tag="VG274_09960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWE07023.1"
                     /translation="MRPLRFLLPLFAAVALSTAAAQAAMETSAEHALLMDGESGQVLW
                     AKDADTPSPPASMSKLMTLELLFQRLRDGRVKLTDTFAVSQRAWSTQGSKGFAMIGSR
                     IPVETLIRGIIIASANDMCVVVAESLGGSVEAFADMMNARARQLGLTHSHFVNPDGLP
                     DPPGQMMSALDLAKLARHLIYEYPQYYHYFAERSFTMDVGNGRSITQQNRDSVLDKFP
                     GTDGLKTGHTDAAGYGITVSAVQNGHRLILVLNGLRYPQLDKMTPAAQDWHGVQLRGD
                     EAARLMGVAFREFRLYSLFKPGAAVGEAQVWQGERDSVPLTVAAPFDVTLQVESRPGM
                     KVTLVYDGPVRAPIEKGQKIGTLRVTAPDFPGAQAPLVAAESVSRANVFARIYLGLRA
                     LLVGQSGG"
     gene            complement(4859..5782)
                     /locus_tag="VG274_09965"
     CDS             complement(4859..5782)
                     /locus_tag="VG274_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWE07024.1"
                     /translation="MGMRGAMRQVAIVCASAAFAAACSTAPPESPTVSLPSGTGVYKI
                     GEPYEQNGVWYQPHVQPDYDETGVASWYGPGFHGQRTANGEIFDAAGLTAAHPTLPMP
                     VNVRVTNLDNGRSLVLRVNDRGPFARGRIIDVSARAARLLGFYGKGTANVRVTYLARA
                     DMPGAAPAETAPEIAAAAAAPAAEVETAALDPVADAPATEQVPQSPPADSQPESAVSD
                     SQPAAVAAGVAAVTHIYVQAGAFAARENAERMQSRLSALGNSSISSIDRNGRLLYRVR
                     IGPFDDVGAADAALARVSGFGSSDAKIVVDQ"
     gene            5985..6299
                     /locus_tag="VG274_09970"
     CDS             5985..6299
                     /locus_tag="VG274_09970"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWE07025.1"
                     /translation="MATLGYDRRARKLLTATERAAAELEIALAPLAWPVITGTGGARK
                     ARAARGGKGKSGGARVIYYVVIRRNVLYLIDIYAKSDKEDLTDADKKEIRKLTAALQA
                     AP"
     gene            6304..6588
                     /locus_tag="VG274_09975"
     CDS             6304..6588
                     /locus_tag="VG274_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE07026.1"
                     /translation="MGRRIVTGLRHALAHARGEIELSEYTVTVPESVDVAGLRKALGL
                     SQSAFARRFGLDVTAVHAWEQDRRRPDRAARVLLAVIAKEPEAVRRALAQ"
     gene            6682..6771
                     /locus_tag="VG274_09980"
     tRNA            6682..6771
                     /locus_tag="VG274_09980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6716..6718,aa:Ser,seq:tga)
     gene            complement(6831..7130)
                     /locus_tag="VG274_09985"
     CDS             complement(6831..7130)
                     /locus_tag="VG274_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein, HigA family"
                     /protein_id="HWE07027.1"
                     /translation="MTTRKPATVGEILVGEFMQPMGRTQATLAGAIGVRRKHVNELCN
                     NRPDVNDGNRTFFSKSVYYNPFWLIWRPRSPKILAFFDIVKLSPGGDWCRRPVIC"
     gene            complement(7218..7991)
                     /locus_tag="VG274_09990"
     CDS             complement(7218..7991)
                     /locus_tag="VG274_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07028.1"
                     /translation="MMGTSRILTLAAMFALGVAIPAWPQSGFDSWSRCANARLLPEKR
                     IAYCTRVLDAGGMPNGDIAGLVMLGGIYRDLHQYAQALQAYSRAVAYEAQGTSRYDHS
                     LASPEALVGAYGARAETYAVTGQRDLAMADVASIFRLVPDSAAPYAFRCHIRAAMKAE
                     LDAALADCNEAARRDARATEVLDATGFVQFRLGHLKDAAADYDAALARNSRLAGALFM
                     RGIIKLRDGDAPGGNADIVAAKDIEPAIADHYAEYGVTP"
BASE COUNT         1468 a         2671 c         2748 g         1378 t
ORIGIN      
        1 ggcgtagatc agtcgcacta acttagtccc gaccaagggg gcgcccccaa gcgcgagggc
       61 gctcatggct taccccttac agcagaacct accccctgcg gttgggcatg tgaagtataa
      121 tatcgccaat cgaatttttg gcaaaggccg tatgaatgca cggttcaaaa atcccggcct
      181 cgcccaaacg gtcctctaat cgatggtctg ttatcgtcgc ccagggctgt agacagaaag
      241 ggatgtcaag atgaccagga attggtggac gcggctgtgc cgcaccctgt cgttgatgct
      301 gttgccgaga cggacctagc acgtgtgcgg cagcgccgcc gtgacgacca tcccgtcata
      361 ggcgggctcg acgccctccg gcagttcgcg ccggagcgtc tcgtagtcca gatcgaagtg
      421 caggttggtg agcaccgcgt gggccggctt cagccgcgcg atccaggcga gcgcggcggc
      481 gacattggcg tgggtgggat ggggcgtgta gcgcagcgca tcgacgatcc agcatgccac
      541 gccttcaagc gccatgaatg cgtcttccgg caacccgttg acatcgcttg aataagcgat
      601 gggcccacaa cgaagcccaa gcgagcggat gctgccgtgc tcctgccaga acgggagcac
      661 cgggatgggg ccgccagccc cctcaatcgc gaattccacg aacggttcgg cgagaacgtg
      721 gctgttgagg atcggcggat agaagctgcc cggcggcgtg gagaagcaat agccgaagcg
      781 ctgctccacg gtatcgagcg ttgtgcggtc gctgtacaca tcgacccgcc gccgcatcgc
      841 atggacgacc gtgcgcagat cgtcgatgcc gtgcagctga tcggcgtggt catgggtgat
      901 gatcacgccg tcgagccagg ccgtgcgcgc gccgagcagc tgctcgcgca tgtcgggcga
      961 agtgtcgacg agcagcgtgg tcacgccctg ggcgccgtgg cgccggacga ggatcgagca
     1021 tcggcggcgg cggtttttcg gattggcggg atcgcaggcg ccccattcgc ccttcccgtc
     1081 cggaccaccg atgcgcggca cgccgcccga cgatccgcaa ccgagtatcg tgacggtcag
     1141 acgatcggtc atgccggccg cgcggcgcgg gtgaacagac gaaagaaatt gtcggtggtc
     1201 gcgcgcgcca gttcgtcgac cgcaacgcct ctcagcccgg ccagcatcgc cgccgtatgc
     1261 acgacgaagg cgggctcgtt gcgcttgccg cgatggggcg tcggcgcaag atagggcgaa
     1321 tcggtttcga cgagcagccg gtccaggggc acgcgctcga gcaccgcgcg cagcgtgtcc
     1381 gacttcttga atgtcgcgat gcccgatgcg gaaatacaaa ggccgagatc gagcgccgcg
     1441 tcggcgagac gctgggtccc ggtgaagcaa tggatgagac cggcgaaggc gccccgcccc
     1501 atctcctcgc gcaggatggc gatcgtgtcg tcttccgcgt cgcgcgtgtg cacgatgagc
     1561 ggcagcgcca gtgtgcgcgc ggcggtgata tgggcgcgaa agtttgcgat ctgcctgggg
     1621 cgcgggccgt gctcgtaata ataatcgagg ccggtttcgc cgatgccgac gaccttggga
     1681 ttccgcgcca gagccagcag cggggccgct tcgtcgagcg gctccttttc ggcctcatgc
     1741 ggatggatgc cgaccgaaca atagatgtcg ggaaagtgtg cggccaccgc ggccacagcg
     1801 gcgaagcccg aaagacgcgt gccgatcgtg acgcatacgc cgacgccggc cgctttcgcg
     1861 cgcgcgacca catcgggcag ctcggctgca aattccggga agtcgagatg gcaatgactg
     1921 tcgacgagca tcgaaaggaa atggacttac agggtgccgc ggcgcgaggc gatcgcctgc
     1981 gcggccgaga caatcgtctg gcgcggatcg agatgcaacg cggccgagcg gataaagctc
     2041 gcgcggatgc ggttgcacaa ttcgacccag cggtcgagat gcggtgcgcc ctcgcgcgcc
     2101 cgggcgagaa tccgcgccgc cagcatgtgc gtcagataga cgccgaacgt ttccatgccg
     2161 cgatcgaact tcgccacgcg ctcggcgagt gcgagggttg cggcgaaatc cggcgccgcc
     2221 gcgcgctcga tcagcttctc ggcttccgcc gcgagcttca atccgtcctc gccgaccagc
     2281 cgtagcgccg cgccgatcga gccgccggac agcgcaacga ggcgcgcgcc atccgccgcc
     2341 gaaacctgcg gcagtcgctc ggcgagcgcg gcggccaaag aggcctcgct caaaggctgc
     2401 aggtgcagcg cctggcagcg cgaacggatg gtcgaagcga gcctcgccgg cgcatgggcg
     2461 agaagcatca acattgcgcg ccgcggcggc tcctccagaa tcttgagcag tgcattcgcc
     2521 gcctgctcgt tcatttcgtc ggccgtgtcg atcacggcga tgcgccagcc gcccgatgca
     2581 gacgtgagtc cgaagaagtt tccgagtttg cggatctcgt cgactgacag cacggtcatc
     2641 ggcttgccgg tatcgggatg gggaccgcgc ctgatggcga gcaatcccgg atgcgcaccg
     2701 gcgcggacca gcgccgcgac cggatcgttt gcgggcagtg aaagatcttc gggccctgcg
     2761 tcggtggcgc cgtagcgcag aaggtagcgc gcaacgcgat aggcaaatgt cgccttgccg
     2821 acgccgggcg gcccgccgat caaccaggcc tgtggcggcc gtccgccgcg gatcgcgcgg
     2881 gcggcgcgtg caagcgcctc gtcctggccg atcaatgttg tcgtctcgcg cggatgcggt
     2941 acgcccgcaa tccggtccgt ctcgatcgtc tcttcgtcgc gcgcgcgctt cgccatcaga
     3001 gtccgaacct tgcggaaacg gtttgccaga tctgcgccgt tacttcatct accggcgcgc
     3061 atgcgtcaac aagcacgcag cggtcgggtt cgctctgcgc gagcgcgcga aaccactccc
     3121 gaaggcgcgc atggaacccc gcatcgaact gctcgaagcg cgtctcggcc cggcctcgcg
     3181 aatcgacgcg cgccagtccc gctgcgggcg caatatcgag gatcagcgtg agatcgggcc
     3241 gcaaggatcc gatcgccgcg gcttcgatgg ccgaaatcgt agcttcgttg aggccgcgtc
     3301 ccgcgccctg ataggcatag gtcgaatcgg tgaaacggtc gcagacgacg cagcggcctg
     3361 ccgcaagcgc cggcctgatc gtgtgggcga gatgatcggc gcgcgcggcg aacagcagca
     3421 gggtctcgga gagcgcgtcc cagcgtcccg gtgcgccatc caccagcaag cgccgaattt
     3481 cttccgcgcc gggcgaaccg ccgggctcgc gcgtcgcgca taccgcgatg ccgcgcgcct
     3541 cgagcgcggc ggcgagacgt ttggcctgcg tggatttgcc ggcgccctcc ccgccttcca
     3601 gcgtgatgaa acgcgcgggc gcggccatca gccgccggat tgtccgacaa gcagcgcgcg
     3661 caggccgaga tagatccgcg cgaacacgtt ggcgcgcgaa accgattcgg cggcgacgag
     3721 aggcgcctgc gcgcccggga aatcgggcgc ggtgacgcgc agcgttccga tcttctgtcc
     3781 cttctcgatg ggggcgcgca ccggtccgtc atagacaagc gtgactttca tgccggggcg
     3841 cgattcgacc tgcagcgtca cgtcgaaagg cgcggcaacc gtcaagggaa cgctatcccg
     3901 ctcgccctgc cagacctgcg cctcgccgac ggcggcgccg ggcttgaaca ggctgtagag
     3961 ccggaactcg cggaaggcga cgcccatgag gcgcgcggcc tcgtcgccgc gcagctgcac
     4021 gccgtgccag tcctgcgcgg cgggcgtcat cttgtcgagc tgcggataac gcaggccgtt
     4081 cagcaccaga atgagccggt gaccgttctg caccgcagaa acggttatgc cgtagcccgc
     4141 cgcgtcggtg tggccggtct tcaggccatc ggtgcccgga aacttgtcga gaacggagtc
     4201 gcggttctgc tgcgtgatgc tcctgccgtt gccgacatcc atcgtgaagc tgcgctcggc
     4261 aaagtagtga tagtactgcg gatattcgta gatcaggtgg cgcgcgagct tcgccaggtc
     4321 gagcgccgac atcatctgtc cgggaggatc cggcaatccg tcgggattga cgaaatgcga
     4381 atgcgtcagg ccgagctgtc tggcgcgcgc gttcatcatg tcggcgaagg cttcgaccga
     4441 accgcccagc gattcggcga caaccacgca catgtcgttg gccgacgcga tgatgatgcc
     4501 gcgtatcagc gtctctacgg ggattcgcga cccgatcatc gcaaacccct tcgaaccctg
     4561 ggtgctccag gcgcgctgcg aaaccgcaaa cgtgtccgtc agcttcacgc gcccgtctct
     4621 cagcctttga aagagcaact cgagcgtcat cagcttgctc atcgatgcgg gcggcgatgg
     4681 cgtatcggcg tccttcgccc acagcacctg tcccgattcg ccgtccatca gcagcgcatg
     4741 ctccgccgag gtctccatgg cggcctgggc ggcggccgtt gacagcgcta ccgctgcgaa
     4801 caacggcaag agaaagcgca acgggcgcat cggcaggaaa ctcctttcgt gatgcagtct
     4861 attggtcgac gacgatcttc gcgtcgctgc tcccgaagcc ggaaacccgg gcgagcgccg
     4921 catcggcggc gccgacatcg tcaaacgggc cgatgcgcac cctatagaga agccggccgt
     4981 ttctgtcgat tgaagaaatc gaggaattcc ccaacgccga aagccggctc tgcatgcgtt
     5041 cggcgttctc gcgcgctgca aacgcgccgg cctgaacata gatgtgcgtg acggccgcca
     5101 cgccggcagc gactgccgcg ggttgactgt cggagaccgc agactcgggt tgagaatccg
     5161 cgggcggaga ttgcggcacc tgctctgttg ccggcgcgtc tgcgaccgga tcgagcgcgg
     5221 ccgtctcgac ctcggctgcg ggcgctgccg cagcggccgc gatctcgggc gcggtttcgg
     5281 cgggcgcggc tccgggcata tcggcacgcg cgagataggt cacgcgtaca ttcgcagttc
     5341 ccttgccata gaagccgagc agcctggcgg cgcgcgccga gacatcgatg atgcgtcccc
     5401 gcgcgaaagg cccgcggtcg ttgacgcgca ggacgagcga acgcccattg tcgagattgg
     5461 tgacgcggac gttgaccggc atgggcaagg tcgggtgggc ggcggtgagc ccggcagcat
     5521 cgaagatttc gccattggcc gtgcgctggc cgtggaaacc cgggccatac caagaagcga
     5581 cgcccgtctc atcatagtcc ggctgcacat ggggctgata ccagacgccg ttctgctcgt
     5641 agggctcgcc gatcttgtag acgccggtgc ccgaaggaag gctgacggtg ggagactcag
     5701 ggggcgccgt cgagcatgcc gccgcaaacg cggcggacgc gcacacaatc gccacctgtc
     5761 gcatggcacc ccgcatgccc atgagtcagc cctcgaattg ctaaacgata ggttaaggag
     5821 gaggccggct caacgggacg ggagcgaaca ggccgcgaag tatgagcgat gacctgggct
     5881 gcgccgcttg gatatggacc gcgccgccgc ggcggctgac gcctggcgca acgcgtggct
     5941 tttcttcctg taagacattg ccatatagtt gggctatgag ggttgtggcg acgcttggat
     6001 acgaccggcg tgcgcgtaag ctcctgacgg ctaccgagcg ggccgccgcc gagcttgaga
     6061 tcgcgcttgc cccactggca tggccggtga tcacaggcac cggtggcgct cgcaaggcgc
     6121 gagccgctcg gggtggcaag ggcaaaagcg gcggcgcccg cgtcatctac tacgtcgtga
     6181 tccgccgcaa tgtgctgtac ctgatcgaca tttacgccaa gagcgacaaa gaggatttaa
     6241 ccgatgccga caagaaagaa atccgcaagc tcacggccgc gctccaagcc gcgccatagc
     6301 ccggtgggcc gtcgcatcgt cacgggactc cgtcatgcgc tcgcgcacgc gcgcggcgaa
     6361 atcgagcttt ccgaatatac tgtgacagtg cccgagagcg tcgacgtcgc aggcttacgg
     6421 aaagcgctgg gcctgtcgca gagcgctttt gcccggagat tcgggcttga cgtgactgcc
     6481 gttcatgcgt gggagcaaga tcgccgccgc cccgaccgcg cggcccgggt tctgcttgct
     6541 gtcatagcca aggagcctga ggcggtgcgc cgggcgctcg cgcaataaca ggctgctggg
     6601 tgctctcgtg ggcgcctcaa taagctgggg cgcgcaatcc ttgccgttgt gaccgttgcc
     6661 ccgtatgtgg accaaatcgc gggatgggtg gccgagcggt tgaaggcacc ggtcttgaaa
     6721 accggcaggc ccgcaagggt ctcgtgggtt cgaatcccac cccatccacc aaccggagcg
     6781 aacgccgcaa gcggagccca atccctgcta acagcgattt tatcagcgat tcaacagatg
     6841 accggccgtc tgcaccagtc tcctcccggg gagagcttca caatgtcaaa gaacgccagg
     6901 attttagggg aacgaggccg ccagatcaac caaaaagggt tataataaac cgatttggaa
     6961 aaaaaggtgc ggttgccgtc atttacgtcc gggcggttgt tgcaaagttc gttcacgtgt
     7021 ttgcgccgga ctccgatcgc accggccagc gtggcctggg tccgccccat cggctgcata
     7081 aactccccga ccagaatctc cccgaccgtg gctggcttgc gcgtcgtcat cagcctggtt
     7141 cggcatatcc tccgcgcctc ccggctctga ttcgcgaatg gattggtaga tttgagcgat
     7201 ctcttcccag ggtttgacta aggcgtcacg ccatattcgg catagtggtc ggcgatcgcg
     7261 ggctcgatgt ctttcgcggc cacgatgtcg gcgttaccgc ccggcgcatc tccatcgcgc
     7321 agcttgatga ttccccgcat aaacagtgcg ccggccagcc gtgaattgcg cgcgagggcg
     7381 gcgtcgtaat cggccgcggc atctttcaga tgtccgagcc ggaattgaac gaaacccgtg
     7441 gcgtcgagaa cctcggtcgc ccgggcatcg cgccgcgcgg cttcgttgca gtcggcgagc
     7501 gccgcgtcca gttccgcttt catcgcagcg cgaatgtggc aacggaaggc atagggcgcg
     7561 gcgctgtcgg gcacgagcct gaagattgac gccacgtccg ccatcgccag atcgcgctgg
     7621 ccggtcaccg cgtaggtttc ggcgcgcgcg ccgtacgccc ccacgagcgc ttcgggcgat
     7681 gcaagcgaat ggtcgtatcg cgaggttccc tgcgcttcat atgccaccgc gcggctgtag
     7741 gcctgaaggg cctgcgcata ttgatgcagg tcccgataga tgccgccaag catgaccagc
     7801 ccggctatat cgccgttcgg catgcccccc gcgtcgagga cgcgtgtgca ataggcgatg
     7861 cgcttctcgg gcaggagcct ggcatttgcg cagcgcgacc acgaatcgaa accgctctgc
     7921 ggccacgccg gaatagcaac gccgagcgcg aacatcgccg ccaacgtcaa tattcgcgat
     7981 gttcccatca tgactctgtt tcctttgcaa cgcgcagcgc tgccaagcac atgcgttcgc
     8041 gcgcggacct cccccatcgt agccaacgtg agcacacatc ttttcatgtt tcgtaatacc
     8101 gtgcaggacc tcgttttcct cccccatcgt gggggaggtg ccgagcgaag cgaggcggga
     8161 gtggcgcgcg ccgcgcgcgg actcccccct ccggtttgct ccgctccgct gcgcaaacca
     8221 cctcccccac gatgggggag gaaaacgagg tcctgcacgg tatta
//
LOCUS       DASZMC010000483         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2948631,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000483 DASZMC010000000
VERSION     DASZMC010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2948631"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VG274_09995"
     CDS             <1..1032
                     /locus_tag="VG274_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07029.1"
                     /translation="SYINFSGSDAICIAVSTSSDATGSYSRYEYDYSALPDYPKYAVW
                     PDAYYGASNLNGSFAQPCAYDRNAMLAGQGAAQICFTTGEFSLLPSDLDGITAPPSGA
                     PAHYLDLGNTTNELQEFDFHVDFANPAKSSFTGPNNIAVPNFSEACGGFANCVPQPAP
                     GEEVEVLGDRLMFRLAYRNFGDHEAMVVAHNVSPGAGSSAQSAMRWYELRATPVGSGF
                     ALFQSGTYQDKTNSLWMGSAAMDKQGNMALGMSVSGSSRDPGVWYTGRLASDPVGKLE
                     APTVAARGTAVETGDSQRWGDYSSMGVDPSDDCTFWYSQMYYNRNHGGDASGDWDTRI
                     VAFKFDTCQ"
     gene            complement(1072..1698)
                     /locus_tag="VG274_10000"
     CDS             complement(1072..1698)
                     /locus_tag="VG274_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE07030.1"
                     /translation="MKWIVFGDKGSGAFSTEAALAEAEAPYEFREVSLERNAQREPAF
                     LAINPSGKIPAVQLPEGGILTETLAILVTIAERFPSAKLLPPSGTFARAQALRWLAFM
                     ASEIYPMVEISDYPVRFAPEGEESKALRRKAGERIRARLAMVEEAVAGPWLLAEGFSA
                     ADIYAAMFSRWSEARGAHLPKLAVLSRALAERPRIAPIWNRHFPPQAQ"
     gene            complement(1842..2459)
                     /gene="rpsD"
                     /locus_tag="VG274_10005"
     CDS             complement(1842..2459)
                     /gene="rpsD"
                     /locus_tag="VG274_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWE07031.1"
                     /translation="MTKRATSKYKLDRRMGESVWGRNKSPVNKRSYGPGQHGQRRARK
                     LSDFGTQLQAKQKLKGYYANINERQFRRYYQEAARRPGDTGENMIGLLERRLDAVVYR
                     AKFVPTPFAARQVVSHGHVRVNGRRVTVSSYLVKEGDTVELAGKAKDMALVLESVRSP
                     ERDVPDYVIADHGKMTAKFLRTPKLSDVPYAVQMQPHLIVEFYSR"
     gene            2656..2865
                     /locus_tag="VG274_10010"
     CDS             2656..2865
                     /locus_tag="VG274_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07032.1"
                     /translation="MAEVVSTSLSPLERARKYREFARETLTLAESAAKPELKATYISL
                     SQCWRSLAHEAESVAKLDPDKLRQH"
BASE COUNT          550 a          945 c          920 g          534 t
ORIGIN      
        1 tcctacatca atttctcggg cagcgacgcg atctgcatcg cggtgtcgac aagctccgat
       61 gcgaccggca gctacagccg ctacgaatac gattattccg cgctgcccga ctatccgaaa
      121 tatgccgtgt ggcccgacgc ctattacggc gcctccaatc tcaacggctc ctttgcgcag
      181 ccctgcgcct atgaccgcaa cgccatgctg gccgggcagg gcgccgcaca gatctgcttc
      241 acgacgggcg agttcagcct gcttccttcc gatctcgacg ggatcacggc gccgccgagc
      301 ggagcgccgg cgcattatct cgatctcggc aacacaacca atgaattgca ggaattcgat
      361 ttccacgtcg atttcgccaa tcccgccaag tcgagcttca ccggccccaa caacatcgcg
      421 gtgccgaatt tcagcgaagc ctgcggggga tttgcgaact gcgtgccgca gccggcgccc
      481 ggcgaagagg tcgaagtcct gggcgaccgc ctgatgttcc ggctggccta ccgcaatttc
      541 ggcgatcacg aagccatggt ggtggcgcac aatgtttcgc cgggcgccgg ctcgagcgcg
      601 caatcggcga tgcgctggta cgaactgcgc gcgacgccgg tggggagcgg ctttgcgctc
      661 tttcagtcgg ggacctacca ggacaaaaca aacagtctct ggatgggcag cgcggcgatg
      721 gacaagcagg gcaacatggc gctcgggatg agcgtctccg gatcatccag ggaccccggc
      781 gtgtggtaca ccggccggct cgcgagtgat cccgtgggca aactggaagc gccgacggtc
      841 gcggcgagag gcaccgccgt cgaaaccggc gacagccagc gctggggcga ctacagcagc
      901 atgggcgtcg atccgtcgga cgactgcacc ttctggtaca gccagatgta ctacaacagg
      961 aatcacggcg gcgacgcgag cggcgactgg gacacccgca tcgtcgcatt caaattcgac
     1021 acgtgtcagt aaggatccct cgccggcagc tagccgcgga agcgggccat gctattgcgc
     1081 ctgcggaggg aaatggcgat tccagatggg cgcgatgcgc gggcgctcgg cgagcgcgcg
     1141 cgaaagcacc gcgagcttcg gcagatgggc tccgcgcgcc tcgctccagc ggctgaacat
     1201 cgccgcatag atgtccgccg ccgagaatcc ttccgcaagc agccatggac cggcgaccgc
     1261 ctcttcgacc atcgcgagcc gcgcgcggat gcgctcgcct gcctttcttc gcagagcctt
     1321 gctctcctcg ccttccggtg caaagcgcac gggataatcc gagatctcca ccatcggata
     1381 gatttcggac gccatgaagg cgagccagcg caaggcttgc gcgcgcgcga aagttcccga
     1441 tggcggcagc agttttgccg aaggaaaacg ttcggcgatg gtgacgagga tggcgagcgt
     1501 ctcggtgagg atgccgcctt cgggcagctg caccgcggga atttttcccg acggattgat
     1561 ggcgaggaag gcgggctcgc gctgcgcgtt gcgctcgagc gagacttcgc gaaactcgta
     1621 aggcgcttca gcttccgcga gcgccgcttc cgtggagaac gctcccgacc ccttgtcgcc
     1681 gaacacgatc catttcatcg cccgccctcc cctggcacga agaagaaaag attcagcgca
     1741 gtggacgcag aggaagcgca gagggcgcag gaaaaaggag ttgcgcgcct tcttctctgc
     1801 gtaccctgcg cttcctctgc gtccactgcg ctaaaatctt ttcagcggga gtagaattcc
     1861 acgatgaggt gcggctgcat ctgcaccgca tagggcacgt cggaaagctt gggcgtgcgc
     1921 aggaatttcg ccgtcatctt gccgtgatcg gcaatgacgt aatcgggaac gtcgcgctcc
     1981 ggactcctga ccgactcgag caccagcgcc atgtccttcg ccttgccggc gagctcgacc
     2041 gtgtcgcctt ccttcaccag atagctcgac acggtgacgc gccgcccgtt caccctcacg
     2101 tgaccgtgac tgacgacctg gcgcgcggcg aagggcgtgg gcacgaattt tgccctgtag
     2161 acgacggcat cgagccggcg ctccagcagc ccgatcatgt tctcgcccgt gtcgccgggg
     2221 cggcgcgcgg cttcctgata gtaacggcgg aactgccgct cgttgatgtt ggcgtaatag
     2281 cccttcagct tctgcttcgc ctgcagctgg gtgccgaaat cggaaagctt gcgcgcgcgc
     2341 cgctgcccat gctgtcccgg cccgtaggaa cgcttgttga cggggctttt gttgcggccc
     2401 cacacgcttt cgcccatgcg gcggtcaagt ttgtatttcg aggttgcgcg cttcgtcatg
     2461 ttggtccgag aagaatgatc ccgcgggcgg gcgcccgcgc ggaaggcggg tactatagtc
     2521 agagacgata acgtgtcaaa tgccgcaacg ggaactttcc gcggcggctg ttcttatcat
     2581 ttaggcccgc aaattgaatt cggaacctct tgttcggcca tgtgtttcgc ctttgtgggg
     2641 aattaaaggc gaagcatggc cgaagtggtg tcgacgtcgc tttctcccct ggaacgagcc
     2701 cggaaatacc gcgaatttgc gcgggaaacc ctgacgctgg ccgagtccgc cgcgaagccc
     2761 gagctgaaag cgacgtacat ttcgctttcc caatgctggc gcagcctcgc ccacgaagcc
     2821 gaaagcgtcg cgaagctcga ccccgataaa ctccggcagc actgagctcg cgagcaacat
     2881 ccgccgtccc cctccggccc ttcgggccac ctcccccaga aatgggggag gaaaatgttg
     2941 cgcgcattt
//
LOCUS       DASZMC010000484         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3227070,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000484 DASZMC010000000
VERSION     DASZMC010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3227070"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VG274_10015"
     CDS             <1..345
                     /locus_tag="VG274_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07033.1"
                     /translation="AASFQAAIIDVVVDRTRAAAQRFRSEFGGIANHLVVAGGVAANH
                     ALRHALAAFCESEDFTIAIPPPALCTDNAAMIAWTGVERSGLGLFDELSFSPRARWPL
                     QDVAEGIGRHVP"
     gene            complement(366..494)
                     /locus_tag="VG274_10020"
     CDS             complement(366..494)
                     /locus_tag="VG274_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07034.1"
                     /translation="MMHNPNQLAPTGSRVSEVVVEVLSTTVLILAGIIAVAQFAYY"
     gene            753..1553
                     /locus_tag="VG274_10025"
     CDS             753..1553
                     /locus_tag="VG274_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWE07035.1"
                     /translation="MKLATWNINSVRLRLDLVLKFLSEQTPDVLCLQETKVINDLFPA
                     EALRNAGYLHQAISGQKGYHGVAILSRLPFARTEAQRYCGKDDCRHVSAVFENGVELH
                     NFYVPAGADIPDPDLNPKFAHKLNFLREMARAFETRRGRRTARAILTGDLNVAPLEND
                     VWSHKQLLDVVSHTPVETGLLRQAREAFDWIDVTRSFVPEDEKIYSWWSYRARDWQAS
                     DRGRRLDHIWISQGLRGQVTDHAIFKPARGWPRASDHVPVMATLDIEG"
     gene            complement(1589..2740)
                     /locus_tag="VG274_10030"
     CDS             complement(1589..2740)
                     /locus_tag="VG274_10030"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWE07036.1"
                     /translation="MRAAARGSQAPAPFTFRQAMTQQYEFELHTPMLTGVAGTDTRLA
                     RNVPALIPKEPGSAHPLLKPRVQKAQPSLLPPEAIALDAFRLTLVQCRWHIAANVAAA
                     VETREVEALHQLRVALRRLRVALASFGGEFRTPELEAIKLRAKSLTNLLAPARDLDVF
                     TRELLEPAANANGAHDAFAVLRTRAQTARAKAWSDAVVVIAGPRFRMFMADLADIVDR
                     GALALVHGRGASKRVSAFEAPAQRVAERMLAYRQRQACKKARRLEALSVADRHELRIR
                     LKKLRYTSEFFAPFFDRARMEKFIARLSRMQDILGALNDVAVAKKTLDTLISENTLGD
                     GHAGSDLSFAAGIVYGWHLERAAHTWRDAVKRWKKFAGTREFWTAGAAQ"
BASE COUNT          556 a          910 c          935 g          557 t
ORIGIN      
        1 gctgcctctt ttcaggctgc catcatcgat gtcgttgttg accggaccag ggccgcggca
       61 caacggtttc gctccgagtt cggcggcatc gccaaccatc tcgtcgtggc tggcggagtc
      121 gcggccaatc atgcgctgcg ccatgctctc gccgcgtttt gcgagtcgga ggatttcacg
      181 atcgcaatac cgccgcccgc tctttgcacc gacaacgccg caatgatcgc gtggacggga
      241 gtcgaacgga gcgggcttgg attattcgat gagctttcct tctcgccgcg cgctcgctgg
      301 ccgcttcagg acgtggcaga ggggattggc cgacacgtcc catagcaagg cgaaagagca
      361 ggctcttagt agtaggcgaa ctgcgcgaca gcgatgatgc ccgccagaat gagcacggtt
      421 gtggacagaa cttccaccac gacctcgctc acccgcgagc ctgtcggcgc cagctgattg
      481 ggattgtgca tcatggtcgc tctcctgttg cccggccatg cgagtgcgct cttgcgcccc
      541 ttaagccgat cagaaactgt gccggcgcta ccggcttccc acggcggata tgggagccct
      601 ttgcgactgc gcaatgaacg attccgtaag gattcaccca cgaagctgcc agcttctgcc
      661 gtaactcatt gaatcaaatg gacaaagttg gagctattta tgctgcactg caaaattcac
      721 atggacgtac cgccaagaag gcattggccg gcatgaagct ggcgacgtgg aacatcaatt
      781 cggttcggct gcggctggat ctggtgctga agttcttgag tgagcagacc ccggacgttc
      841 tgtgcctgca ggaaaccaag gtcatcaacg atctttttcc cgcggaagcc ctcaggaatg
      901 ccggctatct ccatcaggcg atcagcggcc agaagggtta ccacggcgtg gcgattcttt
      961 cgcgcctgcc cttcgcgcgg acagaggcac agcggtattg cggcaaggac gattgccggc
     1021 acgtttcggc ggttttcgaa aacggcgtgg aactgcacaa tttctacgtc ccggccggag
     1081 cggatattcc cgatcccgac ctcaacccga aatttgccca taagctgaat tttttgcgcg
     1141 aaatggcgcg cgcgttcgag acgcgccgcg gacggcgcac cgcgcgcgcg attctcactg
     1201 gcgatctcaa tgtcgctccg ctcgaaaacg acgtctggag ccacaagcag cttcttgacg
     1261 tcgtcagtca cacgccggtc gaaacaggac ttctgcgcca agcacgcgaa gcgttcgact
     1321 ggattgacgt gacgcgcagc ttcgtgcccg aagacgagaa aatctactcg tggtggagct
     1381 accgcgcgcg cgactggcaa gcgtccgatc gcggacgcag gctcgatcac atctggatca
     1441 gccagggact aaggggccaa gtgaccgatc acgccatttt caaacccgcg cgcggctggc
     1501 cgagagcctc cgaccatgtt cctgtcatgg cgacgctcga catcgaaggg tgatcgcggc
     1561 cgcacttcgg cgacggttca gtaatttcct attgcgcggc gcccgcggtc caaaattccc
     1621 gggtgcccgc aaatttcttc cagcgcttga ctgcgtcgcg ccaggtgtgg gccgcgcgct
     1681 cgagatgcca gccgtaaaca atgcccgccg cgaatgagag gtccgatccc gcatgcccgt
     1741 cgccgagcgt gttttcggaa atcagcgtgt ccagcgtctt cttggcgacg gccacgtcgt
     1801 tcagggcacc aaggatgtcc tgcatgcggc tcaatctggc gatgaatttt tccatgcgcg
     1861 cgcggtcgaa aaagggcgcg aaaaactccg acgtgtagcg cagcttcttg agacgaatcc
     1921 gcaattcgtg gcgatcggcg accgaaagtg cctcgaggcg gcgcgccttt ttgcacgcct
     1981 ggcgctggcg atacgccagc atgcgctcgg cgacgcgctg cgcgggagct tcgaatgcgc
     2041 tgacgcgctt gctcgctccg cggccatgga cgagggcgag tgcgccgcga tcgacgatat
     2101 cggcgaggtc ggccatgaac atgcgaaacc gcggtccggc gatcaccaca acagcgtcgc
     2161 tccacgcctt ggcgcgtgcg gtttgggccc gcgtacgaag cacggcgaat gcgtcgtgtg
     2221 cgccgttcgc gttcgccgct ggctccagaa gctcgcgggt aaacacatcg aggtcgcgcg
     2281 ccggcgcgag gaggttcgtc aaagactttg cccgaagctt gatggcttcg agctccgggg
     2341 tgcggaattc gccgccgaaa gaggcaagcg caacgcgaag ccggcgaagg gctacgcgca
     2401 gctgatggag cgcttccacc tcgcgcgttt ccactgccgc tgcaacattg gccgcgatgt
     2461 gccagcggca ctgaaccagg gtgagacgga aggcgtctaa agcgattgcc tccggcggaa
     2521 gcaatgacgg ttgagctttc tggacgcgcg gcttcaggag agggtgcgcc gagccgggct
     2581 ctttgggaat aagcgcggga acgttgcgcg cgaggcgcgt gtccgtgccg gcgacgccgg
     2641 tgagcatcgg cgtgtgcaat tcgaactcgt attgctgggt cattgcctgt cggaacgtga
     2701 atggcgcggg cgcctggctg cccctcgcag ccgcacgcat catccatgat tccaaactgt
     2761 catcaatggc cgtactctcc gcgtcaacgg tccgcgcgaa gcgcggccga cgcttaaact
     2821 tcagcaggga cccagcttca atcttgtccg gttggggcgc gtccggagcg ggtcagtgac
     2881 gcagccgcgc ccatgcggca acggtgaaca ggcagcgccc gcaggccgct tcggcaggca
     2941 cggccgtggt tttgcaaa
//
LOCUS       DASZMC010000485         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4873426,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000485 DASZMC010000000
VERSION     DASZMC010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4873426"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..2126
                     /locus_tag="VG274_10035"
     CDS             327..2126
                     /locus_tag="VG274_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWE07037.1"
                     /translation="MISSARSAERSVNLFLFSVIAIVVGVVTGFGAVLFRGLIALIHN
                     LFFLGHVSLVYDANFYTPASPWGALVILVPVIGGLGVVFLVRTFAPEARGHGVPEVMD
                     AIFYNDGRIRPVVAAVKSLASALAIGSGSAVGREGPIIQIGASLGSSMGQLIRLEAWQ
                     RITLVAAGAGAGIAATFNTPIGGVIFAVELMMPEVSARTFLPVALATGTATFIGRLFF
                     GPRPAFDVPAVASLSVHPSDAYLLVAYAVLGAVTGAGATAFIRGLHFAEQQFDRIENP
                     YLRHALGMGIVGAMMYAFLRLSGHYQVDGVGYATIEAVMVGGLATAPFLALLFASKLA
                     ATSLSLGSGSSGGIFSPSLYMGATLGGAFGALLQLGFPGAGISVPAFAMVGMAAMVGG
                     ATGAAMTAVTMIFEMTLDYSIVMPMIIAAAISIGCRRVLSAENIYTIKLLARRHFIPK
                     ALHANMFLVRRAADVMDRDVLVLPDSMPFDDFLRLPGHADRLRHVVITHGDRIVGVLR
                     VNTALRQGLEKAFTGVTLGVIASREFTIAREEDVMFNVIGRMWRRKSTMAVVASGGMV
                     PHQSDVVGVITKEHVADSVAESIKPYAASDALL"
     gene            complement(2184..2882)
                     /locus_tag="VG274_10040"
     CDS             complement(2184..2882)
                     /locus_tag="VG274_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE07038.1"
                     /translation="MLSQRQQIEFHEIGTVLEYGHGGRTVFAEGEDAHFVYSVVAGVV
                     RIGRHTDSGRRQVLALMLPGDLFGLPDGGIYVNSAEVACPSTLFRVPWLRLRDLLMRE
                     PAMQLNLLNRVAFDLREAQRRIMILGQQNTTQRLASLLLDFVQHGAFFDEGRSQVAVP
                     LSRFDIADYLGTSAETVARGFARLERGGLVRRIAARVVEICDLDGLRQLQTGKRRSLL
                     RRDQPATAPCRSSR"
BASE COUNT          555 a         1011 c         1027 g          638 t
ORIGIN      
        1 gctcctgttt atcaggcgaa atacaggcga ttcttcggaa agcggcggaa caaagagtca
       61 cactcgccga aaagccgtgc aaatcgccgg aattgcgcga gtccggttct gccgcggaac
      121 aggtgcgctt cgcctgttat cacgagcaga cggcgatgga cggctcatgc caaccgtgct
      181 cggtggccag gcgcgcattg ccgcggcgcc tggcaggacg atcgccagag cggcgtttcg
      241 ggggcgagtg gagagggacg gcttgtaatt tcccgtgtcg gtgtggcacc tcgatcccgt
      301 ctgccagcaa tgcgacggca aatccgatga tcagcagcgc ccgctccgcc gagcgatccg
      361 ttaacctgtt cctgttttcg gtcatcgcaa ttgtcgtcgg ggtggtcacc ggtttcggcg
      421 cggttctctt ccggggactg atcgcactca ttcacaatct ctttttcctc gggcatgtct
      481 cgctcgtcta cgacgccaat ttctacacgc cggcttcgcc gtggggggcg ctggtcattc
      541 ttgtcccggt gatcggcggc cttggcgtcg tctttctcgt ccgcaccttc gcgcccgaag
      601 cgcgcgggca cggcgttccc gaagtcatgg acgcgatctt ctacaatgac ggacgaatac
      661 gtccggtcgt tgccgccgtg aagtcgctgg cttcggccct cgccattgga agcggctcgg
      721 ctgtcgggcg cgaggggccg atcatccaga tcggcgcttc gctcggatcg agcatggggc
      781 agctgatccg gctcgaagcc tggcagcgca tcacgctggt ggcggcgggc gcgggcgcgg
      841 gaatcgccgc gacgttcaac acgccgattg gcggggtgat attcgccgtc gagctgatga
      901 tgcccgaggt cagcgcgcgc acgttccttc ccgtggcgct ggcgacagga accgcgactt
      961 tcatcgggcg ccttttcttt ggccccagac cggcattcga cgtgcccgct gtagcgagtc
     1021 tgtcggttca cccttccgat gcctacctgc tggtggccta tgccgtgctc ggcgcggtca
     1081 ccggtgccgg cgcaacggcg ttcatccgcg ggctgcactt tgccgaacag caattcgacc
     1141 gcatcgaaaa tccgtatctg cgccacgcgc tcggcatggg catcgtggga gcaatgatgt
     1201 atgcgttcct tcgcctgtcc gggcattacc aggtcgatgg cgtcggctat gcgaccatcg
     1261 aagcggtgat ggtgggcggg ctcgccacgg ccccgtttct ggcgcttctc ttcgcgtcca
     1321 agctcgcggc aacctcgctc agcctcggat ctggctcgtc cggcggcatc ttctcgccgt
     1381 cgctttacat gggggcgacg ctcggtggcg cattcggtgc gctcctgcag ctcggatttc
     1441 ccggcgcagg catcagcgtt ccggctttcg caatggtcgg aatggccgcg atggtcggcg
     1501 gtgcgacggg cgccgcgatg acggcggtaa cgatgatctt cgagatgact ctcgactaca
     1561 gcatcgtcat gccgatgatc atcgccgccg ccatcagcat cggttgccgg cgggttctct
     1621 ccgccgagaa catttacacc atcaagttgc tggcgcggcg ccacttcatc ccaaaggcgc
     1681 tgcacgccaa catgttcctc gtccgtcgtg cagcggacgt gatggacagg gatgtgctgg
     1741 tacttcccga cagcatgcct ttcgacgatt ttctccggct gccgggtcac gccgatcggc
     1801 tgcgccatgt cgtgattacg catggcgacc gaatcgtcgg cgtgctgcgc gttaacaccg
     1861 cgctgcggca gggtctcgaa aaggcgttca cgggtgtaac cctgggcgtc atcgcgagcc
     1921 gggaattcac cattgcgcgc gaggaggacg tgatgttcaa cgtgatcggc cgcatgtggc
     1981 gcaggaaatc gaccatggcg gtcgtggcgt cgggcggcat ggttccgcac cagtccgacg
     2041 tcgtcggtgt catcaccaag gagcatgttg ccgactcggt cgcagagagc atcaagccct
     2101 acgcggcatc cgacgcgctg ctctgagtgt cgatgcgcgc ggtcgccgca aacgccccga
     2161 ctgcgcagat ccggcgaaat tggctagcgc gagctacgac acggtgcggt cgccggttgg
     2221 tcccggcgaa gcaaggaacg gcgtttgcct gtctggagtt ggcggagacc atcgagatcg
     2281 cagatttcaa cgacgcgcgc agcgatgcgg cgcacaaggc cgccgcgctc cagtcgcgca
     2341 aatccgcggg cgacagtttc cgcggaggtg cccaaatagt ccgcaatgtc gaatcgggag
     2401 agcggcacgg cgacctgtga gcgcccctcg tcgaaaaagg cgccatgctg gacaaagtcg
     2461 agcaggagcg acgccagccg ctgagtggtg ttctgttggc ccagaatcat gatgcgccgc
     2521 tgggcttcgc gcaaatcaaa tgcgactcgg ttcagcaaat tgagttgcat cgcgggctcg
     2581 cgcatcagca ggtcccgcaa tcgcagccac ggcacgcgga agagcgtcga cgggcacgcg
     2641 acctcggccg aattcacata aattccaccg tccggaagtc cgaacagatc gcccggaagc
     2701 atgagggcga gtacctggcg ccgtccgctg tcggtgtgcc ggccgattcg aaccaccccg
     2761 gcgaccacgg aatagacgaa gtgtgcgtct tcgccttcag cgaaaaccgt tcttccgcca
     2821 tgtccatatt cgagcacggt tccgatctcg tgaaactcga tctgctgcct ctgcgagagc
     2881 aaatttccga tgcgtgattg aagatcatgt tcgcgatcaa atacgcgggc gcgaatttcc
     2941 ggtagtcttt gcctcggggg ggatttcgtg gaacgggaca tcttccttgg cctgccgacc
     3001 atgtcgctgc acccgctgtg tcatcttgca gccgcactat agcggaacgg ctgacttcat
     3061 tcagcgcgca gcggccaaag cgctgggccc cgggtccata tccgggatac cggccaaaat
     3121 gcgtactcaa agctgctcga ccgaactcaa cgcaaacgtc tcgcccggcg gattttcgcc
     3181 aatgacatag agacgagcga ggagcggccg ctacgacgct ctcttgcgtt c
//
LOCUS       DASZMC010000486         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3749538,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000486 DASZMC010000000
VERSION     DASZMC010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3749538"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VG274_10045"
     CDS             complement(<1..188)
                     /locus_tag="VG274_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HWE07039.1"
                     /translation="MAELAELFQLSAAERMQLAEDLWDSVTVDPRQIPPLTEAQLAEI
                     ERRLAEHAKNPSSALPWE"
     gene            484..888
                     /locus_tag="VG274_10050"
     CDS             484..888
                     /locus_tag="VG274_10050"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HWE07040.1"
                     /translation="MKAISLLLALAIPAGLALIACTEAGSGTSKDSFVAASLLASCTH
                     SVSDARNQKDKGDLELVCATYFRGLTDAMFVMQALADHGTRTCLPQDEAVGIPEARAV
                     FQTYVRDHPESASNSAGLVATMALVGVHKCMQ"
     gene            complement(1645..2826)
                     /locus_tag="VG274_10055"
     CDS             complement(1645..2826)
                     /locus_tag="VG274_10055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HWE07041.1"
                     /translation="MTRRPLLRLKPREGRRARAGAPWIFSNEIATDLKSLPPGALVDM
                     QGDDGFEFGTGYCNAGSLIAVRVLDAAPGMEIDRQFFAARIGRALSLRETLFDASFYR
                     LVHSEGDRLPGLSIDRFGDTCVVQITTSGMEALIDPILQALDEVLKPTNVVLRADSPS
                     RALEGLSNYVRAAKGEVPVHLGLEENATRYFADLLGGQKTGWYFDQRDNRAFMASLAP
                     NKTVLDVYCHSGGFALAAARAGATEVSGLDSSAPALALAEDGAAGNAASGICRFVKVD
                     AMEELERLASAREAFDIVICDPPPFVKSRKDLEAGARAYRKLARLAARVIAPGGFLLL
                     ASCSHNIAMERFQIECAAGIARAGRRASLIRQASAGPDHPIHPMLPETAYLKALVYAV
                     E"
     gene            complement(2832..>3548)
                     /locus_tag="VG274_10060"
     CDS             complement(2832..>3548)
                     /locus_tag="VG274_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07042.1"
                     /translation="CSDCHRADFAGRLSKGAEPLRVWSSNLRLSAARMSDGEFERALR
                     RGIASDATSLWAMPSADYAYMSEDDVAAILAYLRAMGAAGPTRPRPVWDRDARLALLE
                     GRIVPAVLAVRDAPSSLDLGPRYDGGRYLARISCSECHGADLAGTPERSIPDLDIVYL
                     YSRPAFFDLLRRGIGAHHRRVPAMMRLSSIRFHVLADYEIMALYDYLDARAHAPPALR
                     AREKANELRRRAAIEAQSEE"
BASE COUNT          730 a         1051 c         1093 g          674 t
ORIGIN      
        1 tcctcccaag gcagcgcgga ggacggattc ttggcatgct cggccaaacg tcgctcaatt
       61 tcggccagtt gagcttccgt cagcggcgga atctgacggg gatcaacagt cacgctgtcc
      121 cataagtctt ccgcaagctg cattcgctcg gcggctgaca gttggaacaa ttctgctagc
      181 tcggccattg tatacctccg tcgctaggca aattctccct caaatgcttc gcgcaaacaa
      241 ggtcgacaac ccatgagcac tcgaggcttc ccaagtcacg cgtgaatcgt tttcggtgtc
      301 gtcgcgccga acttggctgg acggagaaat cacaagacgc ctccagttct ggcgatgact
      361 gcgtccctgc tcgcgaagaa ccgcgcaata acgagatttt agcaatcgta gcgcaatttc
      421 ggccaatcac cgcgcaagcg gtccagccca tgcatatcga cgcattcaaa tgcggagcgc
      481 agtgtgaaag cgatctcgct cctattggcg ttggcaatac cagctggcct ggctctcatc
      541 gcatgcacgg aagcaggatc gggcacctcg aaggattcgt tcgtcgcagc atctcttctt
      601 gcgtcctgta cgcattctgt aagcgatgcc aggaaccaaa aggacaaggg cgacctcgag
      661 ctggtttgtg cgacgtattt tcgaggcttg acggatgcca tgttcgtcat gcaagcgctc
      721 gcggatcatg ggacacgaac gtgtctgccg caagatgaag ctgttggcat tccggaggcg
      781 cgcgccgtct tccaaaccta cgtgcgagat catcctgaga gtgcatcgaa ttcggcgggg
      841 ttggtcgcca caatggcttt ggttggcgtc cacaaatgca tgcaatgaat gaactgggcg
      901 gctgcatcgt tggaaacgac ttgtcagaca gaaacgtccg tctttggcac gaagcttccg
      961 aatacactcg gcggctttga gagaggcgct acaacaaaca acgagcctac cagtagtgga
     1021 atgggttgta cgatcaggta ttacagcgct tcgtctggaa aggcgacggt ctttgtctac
     1081 aacaaatgta ttatgcttat tccgaatggc ccaatggacc cgctaatccg acgcgaattc
     1141 gaccaggcga gtcgagacat actgaggtcc agtcaatatt ctcatcggac attcgaattg
     1201 atcgaacagt acgccacagg cagcccgggc gacggtccag aatttctttg ctccgagttt
     1261 ctggagcggt cctctagtag tacggtgcgg acgtttctgt atgtcaccgg agcgcgcggc
     1321 agtttcgtaa agctcagagt gacactgacc accgacgatc cgcgcgactc cacggcgcga
     1381 gatttcgcgg attccatcgc gaccattctg cgaagtcgga cgaaagcggt caattaggat
     1441 cggactcgtt tccacgtccg cgagcgccgg ccgaggcaaa gacggccttc gctgtcgccg
     1501 ctaccttatt ccaagttcgc ggcggttgtt ttgtgaaccc ccgaagaatg acgaatccat
     1561 agttgggttg acgatgctgt ttcacacgct atgcgaaatg attggtgatg acccagccgg
     1621 tctcgatgcg cttgctgcca gcgcttattc caccgcgtac acaagagctt tgaggtaggc
     1681 ggtttcgggc agcatggggt ggattggatg gtcggggccg gcgctggcct gccgaatcag
     1741 acttgccctg cggccggcgc gcgcgattcc ggcggcgcac tcgatctgaa agcgctccat
     1801 cgcgatgttg tgcgagcagg aggcgagcag cagaaagccg cccggcgcaa tcacccgcgc
     1861 cgcgagccgc gccagcttgc gataggcgcg ggcgcccgcc tcgaggtcct tgcgcgattt
     1921 cacgaagggc ggcggatcgc agatcacgat gtcgaacgcc tccctggccg aagcgaggcg
     1981 ttccagctct tccatcgcat cgaccttgac gaaacggcag atgccgctcg cggcgttgcc
     2041 cgccgcgcca tcttcggcga gagcgagcgc cggtgcggag ctgtcgagac cgctcacctc
     2101 cgtcgcgccg gcccgggcgg cagctagggc gaacccgccg gaatggcaat agacatcgag
     2161 tacggtctta ttgggcgcca gcgacgccat gaacgcgcgg ttgtcgcgct ggtcgaaata
     2221 ccatcccgtc ttctgcccgc ccaagagatc ggcgaaatag cgcgtggcgt tctcttccag
     2281 gccgagatgc actggcactt caccttttgc cgcccgcaca taattcgaaa gcccttcgag
     2341 cgcgcgcgaa ggcgaatccg cgcgcagaac cacgttcgtg ggcttgagga cttcgtcgag
     2401 cgcttgcagg atgggatcta tgagcgcttc catgccggat gtggtgatct gcaccacgca
     2461 ggtgtcgccg aaacgatcga tggataggcc gggcaaccgg tcgccctctg aatgaacgag
     2521 gcgatagaag gacgcatcga agagggtttc gcgcagagac agtgcgcggc cgatgcgggc
     2581 ggcgaaaaac tgccgatcga tctccatgcc cggcgccgcg tcgaggacac gaacggcgat
     2641 caggctgccc gcgttgcaat agcccgtacc gaactcgaat ccgtcgtcgc cttgcatatc
     2701 gacgagcgcg ccgggcggca ggcttttcaa atcagtcgca atttcgttcg agaaaatcca
     2761 tggggctccg gcgcgggcgc ggcggccttc gcgtggcttg agacgaagca gaggacgcct
     2821 ggtcacgcgg attattcctc ggactgcgct tcgatcgccg cgcgacgcct caactcgttg
     2881 gccttctcgc gcgcacgcag agccggcggc gcgtgagcgc gtgcgtcgag atagtcgtaa
     2941 agcgccatga tttcgtaatc cgcaagcacg tggaagcgaa tcgaagacag acgcatcatc
     3001 gcaggaacgc ggcgatgatg cgcgccaatg ccacggcgaa gaagatcgaa aaaggcgggg
     3061 cggctgtaga ggtaaacgat gtcgagatcg gggatgcttc gctccggcgt tccggcgaga
     3121 tcggcgccgt ggcattcgct gcaggaaatt cgtgccagat atcgcccgcc atcgtagcgc
     3181 ggaccgagat cgagggagga cggcgcgtcc ctgacggcga gcacggccgg aacgatgcgt
     3241 ccttcgagga gtgcgagccg cgcatcacga tcccaaacgg gccggggcct ggttgggcct
     3301 gcggcgccca tcgcacgaag ataagcgagg atcgccgcga catcgtcttc gctcatatag
     3361 gcgtaatcgg ccgaaggcat cgcccacagg ctcgttgcgt ccgatgcgat gccccggcgc
     3421 agcgcacgtt cgaactcgcc atccgacatg cgcgccgcgg aaagacggag atttgacgac
     3481 catacgcgca acggctcagc gccttttgac agccggcctg cgaaatctgc gcggtgacaa
     3541 tcgctgca
//
LOCUS       DASZMC010000487         5176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2678410,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000487 DASZMC010000000
VERSION     DASZMC010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2678410"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..3128)
                     /gene="polA"
                     /locus_tag="VG274_10065"
     CDS             complement(174..3128)
                     /gene="polA"
                     /locus_tag="VG274_10065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWE07043.1"
                     /translation="MNDAPSADRPIAKGDHLYLVDGSGYLFRAYHALPPLSRKTDGLP
                     TGAVSGFSNMLWKLLEDMRAGDRPTHLAVIFDAGRTTFRNAIYPLYKANRPDPPEDLI
                     PQFPLVRAATRAFGVACIEEAGFEADDLIATYARLAREAGARTTIVSSDKDLMQLVID
                     GEIELFDTMKNKRIASAEVIEKFGVPPSKVVEVQALAGDSTDNVPGVRGIGVKTAAEL
                     INAFGTLEALLTRTAEIKQPRRRETLIENAQNARISLKLVQLDYHVPIKTQIDDFAVH
                     DPEPGALIGFLKAMEFSALCRRAASHFGVDDVDAVPPAPDPAQPALAAPGEMAPLPPR
                     AKAPLPAEKSVGLAKEPPRGGTPGAVMDRALLLKPIDHDAYETVIALERLHHWIARAR
                     EAGRVCVDTETTSLDAMQAQLCGVSLAVAAGEACYIPCGHRTGSGLDLRGEGAIVQLD
                     ETDVLERLKPLLEDPGVLKIAQNLKYDYLIFLQRGIRIAPFDDTMLISYVLGTALHGH
                     GMDELSGLHLQHKPITFNEVTGTGKDHINFDCVPLKEATRYSAEDADVTFRLHALLKP
                     RLVAEAMVTVYETLERPLVPVLADMERAGVMIDPERLRRLSNDFGRDMVKLEDQIHKL
                     AGGGFNIGSPKQLGDILFGKFKLEGGRKTKMGAWSTDADALEELAAQGHEIARLVLDW
                     RQLQKLRGTYTDALPGFINPQTGRVHTSYAMASTSTGRLASTDPNLQNIPIRTEEGRR
                     IRAAFIAPPGMTLISADYSQIELRLLAHIADIPELKQAFAENLDIHAMTASEIFGVPV
                     KGMPAEVRRRAKAINFGIVYGISAFGLANQLGIARSEADDYIRKYFERFPGIRSYMEA
                     TKELARDKGYVKTLFGRRIHIREIRSKIPGLRGGAERAAINAPIQGTAADIIRRAMIR
                     VPPALAQKKLDARMLLQVHDELVFEAAGKDAAPTCAAAKRVMEKAALPAISLSVPLTV
                     EARAAANWDEAH"
     gene            3128..3964
                     /locus_tag="VG274_10070"
     CDS             3128..3964
                     /locus_tag="VG274_10070"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWE07044.1"
                     /translation="MGMRPRAQDESAVAIRTLDGGLVAHLEVDARVAQRAAAPVAGDA
                     GVIDFDNLRGIDGHGNVLLRRAAIIAAVRGLATREAWPVGAGALASAMVILNRIYTRT
                     GDKGETALGDGTRLPKHAPRIAAYGTIDEANAAIALARLHVSGEIDAMLARIQNDLFD
                     LGADLCVPETSRRSEGALRIVDSQVERLEREIDAMNGELAPLASFVLPGGTPAAAYLH
                     LARTIVRRAERQIVELAGNETVGAGALEYVNRLSDHLFVVSRFVNDKGSKDVLWIPGA
                     NR"
     gene            complement(3965..4327)
                     /locus_tag="VG274_10075"
     CDS             complement(3965..4327)
                     /locus_tag="VG274_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07045.1"
                     /translation="MRRHRAPDSVRGKRALEAAMGVILRFVSLVLLVVALMLLGADAV
                     TSLEKGGQVTVRSIDQVWSLLNPAGITSFKAWLEHSLPSPLPGWFYAVLALPAWALTG
                     VLGVVLAFLFGRHAPEQA"
     gene            complement(4373..4837)
                     /locus_tag="VG274_10080"
     CDS             complement(4373..4837)
                     /locus_tag="VG274_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWE07046.1"
                     /translation="MPKIDVLSVAERKGSGYPAPFHLKAGDRVRQALGDAGGLQDFGV
                     NLLRLPPGAWSSQRHWHSAEDEFVYVLSGEVTLVADAGEQVLRAGDCAAFPRNHDDGH
                     HLINQSTGPAVVLEIGTRSTEDICEYPDIDMRIDARIGHYTHRDGTPYPERK"
     gene            complement(4898..>5176)
                     /locus_tag="VG274_10085"
     CDS             complement(4898..>5176)
                     /locus_tag="VG274_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWE07047.1"
                     /translation="KLAKGTRVLDKTVNIGEAKTNRSRLISSVEAGEEIVIARAGEPV
                     ARLVPIKPKKKKRSDRRPGYLKGRIWSAPDFDDPLPDDILSAFRGERD"
BASE COUNT          932 a         1603 c         1690 g          951 t
ORIGIN      
        1 cagcgaaatc tgcgagccga acgagaacaa cagatcgtag gcgtgatgga ggatgccggt
       61 gaggccggcc gcgatgcgtt ccatggacgt cgtccgcgcg tgcgagagga gcggctgtcg
      121 ccgcgcgggt attctggtgg aacgcgatta acgatttgtt aagggggagc cgctcaatgc
      181 gcctcgtccc aattggcggc ggcacgcgct tcaacagtca gaggcaccga aagagagatc
      241 gcgggaagcg cagccttctc catgacgcgt tttgcagcgg cgcaggtggg ggccgcatcc
      301 tttcccgccg cttcgaagac aagttcgtcg tgcacctgca gcagcatcct ggcgtcgagc
      361 ttcttctgcg ccagcgccgg cggcacgcga atcatggcgc ggcggatgat gtcggcggcc
      421 gttccctgaa tgggtgcatt gatggccgcg cgctccgcgc cgccgcgcag acccgggatc
      481 ttcgaacgga tttcgcggat atgaatgcgc cgcccgaaca gcgtcttcac atagcccttg
      541 tcgcgcgcca actctttggt cgcctccatg tagctgcgga tgccggggaa ccgctcgaaa
      601 tatttgcgga tatagtcgtc ggcttcgctg cgcgcgatac cgagctggtt ggcgagaccg
      661 aaggccgaga tgccgtagac gatgccgaaa ttgatcgcct tggcgcggcg gcggacttcg
      721 gcaggcatgc ccttcaccgg cacgccgaaa atctccgatg ccgtcatggc gtggatgtcg
      781 agattttccg caaacgcctg cttcaattcg ggaatgtcgg cgatatgggc caaaaggcgc
      841 aattcgatct ggctgtaatc ggcgctgatg agcgtcatgc cgggcggcgc gatgaaggcc
      901 gcgcgaatgc gccgtccttc ttccgtgcga atgggaatgt tctgcaggtt cggatcggtg
      961 gaggcgagcc ggcccgtgga cgtcgacgcc atcgcgtatg aggtgtgcac gcggccggtc
     1021 tgcggattga taaaacccgg cagggcgtcg gtgtaggtgc cgcgcagctt ctgcaattgc
     1081 cgccagtcga gaaccagcct ggctatctcg tggccctgcg ccgccaattc ctccaatgcg
     1141 tcggcatccg tcgaccaggc gcccatcttc gtcttgcggc cgccctccag cttgaatttg
     1201 ccgaacagga tgtcgcccag ctgtttgggc gagccgatgt tgaatccgcc gcccgcaagc
     1261 ttgtgaattt gatcttcgag cttgaccatg tcgcggccga aatcattcga cagcctgcgc
     1321 agccgctcgg gatcgatcat cacgccggca cgctccatgt cggcaaggac cggcacgaga
     1381 ggccgttcga gcgtctcgta gaccgtgacc attgcttcag ccaccaggcg cggcttcagc
     1441 aaggcgtgca ggcggaacgt gacatcggcg tcctcggcgg aatatctggt ggcctccttg
     1501 agcggcacgc agtcgaaatt gatgtggtcc ttgccggtgc ctgtgacctc gttgaaagtg
     1561 atcggcttgt gctgaagatg cagcccggaa agctcgtcca tcccgtgtcc gtgcagcgcc
     1621 gtgccgagca cataggagat gagcatggtg tcgtcgaaag gcgcgatgcg gatgccgcgc
     1681 tggaggaaga tcagataatc gtatttgagg ttctgcgcga tcttgaggac gcccggatct
     1741 tcgagaagcg gcttcaatcg ctccaggacg tcggtttcgt cgagctggac gatggcgccc
     1801 tcgccgcgca ggtcgagccc gctgcccgtg cggtgaccgc atggaatgta gcaggcctct
     1861 cccgccgcca ccgcaagcga tacgccgcac aattgcgcct gcattgcatc cagagatgtc
     1921 gtttcggtgt ccacgcaaac gcggccagcc tcgcgggcgc gggcgatcca gtgatgcagc
     1981 cgctcgagcg cgattaccgt ctcgtaggcg tcgtgatcga tcggcttgag cagcaacgcc
     2041 cggtccatca ccgcgcccgg cgttccgccg cgcgggggtt ccttcgccaa accgacagat
     2101 ttttcggccg gaagcggcgc ctttgcccgc ggcggaagcg gcgccatctc gcccggtgct
     2161 gcgagtgccg gctgcgcagg atcgggcgcg ggcggcaccg cgtctacgtc gtcgacgccg
     2221 aaatgcgagg cagcgcggcg gcaaagcgcg gagaactcca tggccttcag aaagccgatc
     2281 agcgcccccg gttcgggatc gtggaccgca aagtcatcga tttgagtctt aatggggaca
     2341 tgatagtcga gctgcaccag tttcagcgag attcgcgcgt tctgggcgtt ctcgatcagc
     2401 gtttcgcggc gcctgggctg cttgatttct gccgtgcggg tcaacagcgc ttcgagcgtg
     2461 ccaaaggcgt tgatcagttc ggcggccgtc ttgacgccga tgccgcgcac gccgggaaca
     2521 ttatcggtcg aatctcccgc gagcgcctgc acctcgacga ccttcgaagg gggcacaccg
     2581 aatttttcga tgacttccgc cgatgcgatg cgcttgttct tcatcgtgtc gaacagctcg
     2641 atctcgccat cgatgacgag ctgcatcagg tccttgtcgg aggaaacgat cgtggtgcgc
     2701 gcgcctgctt cgcgggcgag acgcgcatag gtggcgatca ggtcgtcggc ctcgaagccg
     2761 gcctcctcga tgcaggcgac gccgaaggcg cgggtggcgg cgcgcaccag cggaaattgg
     2821 gggatcaggt cctcgggcgg gtcgggccgg ttggccttgt agagtgggta gatcgcattg
     2881 cggaatgtcg ttctgcccgc atcgaaaatg accgcgagat gcgtgggccg atcgccggcg
     2941 cgcatatctt cgagcagctt ccagagcatg ttcgaaaagc cggacacggc gcccgtcggc
     3001 agcccgtccg ttttgcgtga cagcggaggc agcgcgtgat aggcgcgaaa caggtagccg
     3061 gaaccgtcga cgagatagag atggtcgccc tttgcaatcg ggcgatctgc ggacggggcg
     3121 tcattcaatg ggcatgcgcc cccgcgcgca ggacgaatcg gcggtcgcaa tacggacact
     3181 cgacggaggt ctcgtcgccc atctcgaggt agacgcgcgg gtggcccagc gtgccgccgc
     3241 ccccgtcgca ggcgacgcgg gtgtcatcga cttcgataat ctcaggggca tcgacggcca
     3301 tgggaatgtc ctgctgcggc gggcggcgat catagccgcc gtccgcggtc tcgcaacccg
     3361 cgaggcttgg cccgttggcg ccggcgcgct agcctccgcc atggtcattc tcaaccgcat
     3421 ctacacacga acgggcgaca agggcgaaac cgcgctcggc gacggcacaa ggctgcccaa
     3481 gcatgcgccg cgtatcgcag cttacggcac catcgacgag gccaatgcgg cgatcgctct
     3541 cgcccgtttg catgtcagcg gcgaaatcga tgcgatgctg gctcgtatcc agaacgacct
     3601 cttcgatctc ggcgcagatc tttgcgttcc cgagacgagc cggaggagcg agggcgctct
     3661 gcgcatcgtc gactcgcagg tcgagcggct cgagcgcgag atcgacgcga tgaacggcga
     3721 actggccccg ctcgcgtcct ttgtgctgcc gggcggaacg cctgctgcgg catatctgca
     3781 tctcgcgcgc acgatcgttc ggcgcgccga gcggcagatc gtggagctgg ctggaaacga
     3841 aacggtgggc gccggcgccc tcgaatatgt gaaccggctg tccgaccacc ttttcgtcgt
     3901 cagccggttc gtgaacgaca agggcagcaa ggacgtgctc tggatacccg gcgccaaccg
     3961 ctgatcaggc ctgttcgggc gcatgccgcc caaaaagaaa cgccagcacc acccccagga
     4021 cgccggtcaa tgcccaggcc ggcagtgcga gcacggcgta gaaccatccc gggagtggag
     4081 aaggcaggct gtgctcgagc cacgccttga acgacgtgat gccggcagga ttgagcagcg
     4141 accacacctg gtcgatcgag cggacggtca cctgcccacc cttttcgagc gaggtcacgg
     4201 catcggcgcc cagaagcatg agggcgacca ccagcaggac aagactgacg aaacgcagga
     4261 tcacgcccat cgccgcctca agcgcgcgtt tgccgcgaac gctatcgggc gcgcgatggc
     4321 ggcgcaagaa agcggccggc ggtcctcata gcgtccgaag ggagccggct cgtcacttgc
     4381 gctcgggata aggcgttccg tcccggtgcg tgtagtggcc gatgcgggca tcgatccgca
     4441 tgtcgatatc gggatattcg caaatgtctt ccgtggagcg cgtgccgatt tcgagaacga
     4501 ccgccggccc cgtgctctgg ttgatcagat ggtgcccgtc gtcgtgattc ctgggaaacg
     4561 ctgcgcaatc ccccgcgcga agaacctgtt cgcccgcgtc ggccaccagc gtgacctcgc
     4621 ccgacaggac atacacgaac tcgtcttcgg cgctgtgcca gtgacgctgg ctcgaccagg
     4681 cgcccggagg caggcgaagc aggttgacgc cgaaatcctg caggccgcct gcgtcgccca
     4741 gcgcctgacg cacgcgatca ccggccttca gatggaacgg agcggggtag ccggatcctt
     4801 ttcgctcggc aacagagagc acgtcgattt tgggcatggc cttcgatcct tcctacgaga
     4861 gtacgtgtga accgtgagtg agaatcaaga aggaatttca atcccgttcg ccacggaacg
     4921 cggagagaat gtcgtccggc aacgggtcat caaaatctgg tgcgctccaa atccttccct
     4981 tcagatatcc aggtcggcga tctgaacgtt ttttcttttt cggcttgata ggtaccagcc
     5041 tggcgacggg ttcgcccgct cgcgcaatga cgatctcttc gccagcttct acactggata
     5101 taagcctgga acgattcgtc ttcgcttcgc caatattgac ggtcttgtcc aagacgcgag
     5161 tacccttagc caattt
//
LOCUS       DASZMC010000488         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4875333,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000488 DASZMC010000000
VERSION     DASZMC010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4875333"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..>1769)
                     /locus_tag="VG274_10090"
     CDS             complement(242..>1769)
                     /locus_tag="VG274_10090"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWE07048.1"
                     /translation="QMAYVADEFAGGKGHLTTRQNIQYHFVPLARVADLLQSLADVHL
                     TTREACYNTVRNVTGCPLAGIHPEEPFDVQPYARRVAFAFLHKELTDNLPRKFKIAFS
                     ACPEDCIATAINDCGLRGIIRDGVRGFSMTVAGGLGPLPTEAKLLHEFIPADDLLHRI
                     EAVIRVFNEHGNRKNKNTARLKFVMRTRGFDWLRDTIEEQYQDIIKNGGIALPAEVPD
                     SFGGFKAQKPPLGSGALLPVWEPATPEFAEWRQTNVMAQKQKGYNIVTVTTPQGNLTG
                     EQMRGLADVADQAGDGFLRFSMGQNVQFGWIGEGSLQKFYGALQKLGLADGHADEISD
                     PISCPGAYSCNLGLTKTMGLGEAIAAAMKDETDALVRKLRINSSGCPNSCGQHWIADI
                     GFYGNARKIEVPEGGKKEVPHYQMMLGGTQHEFGVQIMSVPAKLAPVAAKRVLEHYKI
                     HRQPGDTFREYVLRHKVETFKELLADLAKPPADVPEMFRDWGDDMEYSLKLGRGECAA
                     "
BASE COUNT          327 a          550 c          523 g          369 t
ORIGIN      
        1 cctctagccg catgagtccc ggctttaagt cgcgcgaaag agactggaga ttagcctgaa
       61 cggacgcttg gcgtcggtgt ctctccgtcg cctggcgaat gcaataaaaa ccaacggcac
      121 acaaaatcaa gataatcgcg atcgcggtta cggccaacgt ccttttcatc ctagacatct
      181 caacgcaaaa aggcccaccg gagttcgtcc gatgggcctt ttatttaaag aggagaagat
      241 tttaggcggc gcactcaccg cggccgagct tcaggctgta ctccatatcg tcgccccaat
      301 cgcggaacat ctcggggacg tcggcggggg gcttcgccaa atcagccagc agctctttga
      361 acgtttccac tttgtgtcgc agcacatatt cgcggaacgt gtcgcccggt tggcgatgga
      421 tcttgtagtg ctccaaaaca cgcttggcgg ccacgggagc gagcttcgcc gggacgctca
      481 taatctgcac gccaaactca tgctgcgtgc cgccgagcat catctgatag tgaggcactt
      541 ccttcttgcc tccctcaggc acctcgatct tgcgtgcgtt tccgtaaaag ccgatatcgg
      601 cgatccagtg ctggccgcat gagttcgggc agccgctgga attaatgcgc agcttgcgca
      661 ccagtgcgtc ggtttcatcc ttcatcgccg cggcgatcgc ttcgcccagg cccatcgttt
      721 tagtcagacc caggttgcag ctatacgcgc cggggcagga gatgggatcg ctgatctcgt
      781 cggcgtggcc atccgccagg cccagcttct ggagcgcacc ataaaacttt tgcagcgagc
      841 cttcgccaat ccagccgaac tgcacgttct ggcccatgga gaagcgcagg aaaccgtcgc
      901 ccgcctgatc ggcgacgtcg gccaggccgc gcatctgctc gcccgtgaga ttgccctgcg
      961 gcgtggtcac ggtgacgatg ttgtagccct tctgcttctg cgccatcacg ttggtctggc
     1021 gccactcggc gaactccggc gtcgcgggct cccatacggg cagcagcgcg cccgatccga
     1081 gcggaggctt ctgggctttg aatccgccga aactatccgg cacttcggcc ggcagcgcga
     1141 tgccgccgtt cttgatgatg tcctgatact gctcctcgat ggtgtcgcgc agccaatcga
     1201 acccgcgcgt gcgcatcacg aacttcaggc gcgccgtgtt cttgttcttg cgattgccgt
     1261 gctcgttgaa tacgcggatc acggcttcga tgcgatgcag caggtcgtcg gcggggatga
     1321 actcgtggag cagctttgct tcggtcggca gcggtcccag gcctcctgcg accgtcatac
     1381 tgaagccgcg aacgccatcg cgaatgatgc cgcgcaggcc gcaatcgttg atcgccgtgg
     1441 cgatgcaatc ctccgggcag gccgaaaacg cgatcttgaa tttgcgcgga agattgtcgg
     1501 tgagttcctt gtgcaggaac gcgaaggcaa ccctacgcgc gtatggctgc acgtcgaacg
     1561 gctcttcggg atgaatgccc gccagcggac atcccgtcac gttgcgcacc gtgttgtagc
     1621 aggcttcgcg cgtggtcaag tgcacatcgg ccagcgattg cagcaggtct gctacccggg
     1681 ccagcggcac gaaatgatat tgaatgttct gacgcgttgt cagatggccc ttgccgcccg
     1741 cgaactcgtc ggctacatac gccatctgg
//
LOCUS       DASZMC010000489         6869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_501234,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000489 DASZMC010000000
VERSION     DASZMC010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501234"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1564)
                     /gene="argJ"
                     /locus_tag="VG274_10095"
     CDS             complement(230..1564)
                     /gene="argJ"
                     /locus_tag="VG274_10095"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWE07049.1"
                     /translation="MAKTRAAPPSRRARSAVRPFTRLVASKSGSTKAAATPVSPLAPK
                     SLAALPPLAGVRLATIAAGIGYRDRDDVLLAVLAPGTQAAGVFTRSKTASASVEWCRA
                     NLGKRTARALVANSGNANAFTGAAGRDAVREVAASAAAALDCPANEVFVASTGVIGEP
                     LPSHKITKIMATLADAGAAGGWRAAAEAIMTTDTFPKMATARAMIEDHRVTINGIAKG
                     SGMIAPDMATLLAFVFTDANLSAPVLQELLSSGVRNSFNAITVDGDTSTNDTVLVFAT
                     GKGAPHPQIAKAGDRRLGGFRQKLDDLLLELALQVVRDGEGAQKLIRIDVSGAETYES
                     ARRVGFAIANSPLVKTAIAGGDANWGRVVMAVGKAGEPADRDRLRIDFCGYLVAESGQ
                     RAASYDEGLASRAVSGREVAIGVDLGVGAGRARVWTCDLTEGYIRVNASYRS"
     gene            1776..4532
                     /gene="secA"
                     /locus_tag="VG274_10100"
     CDS             1776..4532
                     /gene="secA"
                     /locus_tag="VG274_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWE07050.1"
                     /translation="MLGLNENAQRFLGGIGQRFFGSSNERKLRPMQRRVQDINALEPA
                     FEAKSDDDLRGLTATFKARLEAGESLDDLLPEAFATVREAAKRTLGQRHFDVQLVGGM
                     VLNDGRIAEMKTGEGKTLVATLPVYLNALAGEGVHVVTVNDYLARRDAEWMGQIYKFL
                     GLKVGCIVHGLTDEQRHEAYACDVTYGTNNEFGFDYLRDNMKYSIASMVQRGHHFAIV
                     DEVDSILVDEARTPLIISGPTDDQTEIYRAVDVLIPKLEKNDFELDEKQRTVTLTEAG
                     NERMVELLREANLLETGDLYDIENISMVHHVNQALKAHKMFQRDRDYIVKDGKVVIID
                     EFTGRMMEGRRYSEGLHQALEAKENTAIESENVTLASITFQNYFRLYEKLAGMTGTAM
                     TEAAEFMDIYKLDVLEVPTNMPVNRTDSDDEVYRTAGEKDEAIIALVKDCHERGQPML
                     VGTTSIEKSEHLSALLKKRKIRHNVLNARYHEQEAVIVAQAGVSGTVTIATNMAGRGT
                     DIQLGGNADMRIRTELGGVVDEAERTRRIEEIRAEIATDKERAIGAGGLYVVGTERHE
                     SRRIDNQLRGRSGRQGDPGASKFFLSLQDDLMRIFGSDRLDSMLQRLGLEEGEAIAHP
                     WVNKALEKAQQKVEARNFEMRKNILKYDNVLNDQRRVVFEQRREIMSADDVSDQIADM
                     REQIVDDLVARHIPPKAYAEQWDAIGLQAEIEKIFAITLPVIEWTKEEGIADEEVRER
                     MLKAVESRAAERAANVGPDIMRYVEKSVLLQTLDHDWREHIVNLDHLRQYVGLRGYGQ
                     RDPLNEYKSEAFALFEGLLSKLRSDVVRQLMHLQITVEQPPPPIERALPPMRAQHIDP
                     LTGEDEFAPGLVPADQADLRATRIRDVPVDPNDPGTWGKVQRNAPCPCGSGRKFKHCH
                     GALV"
     gene            complement(4551..5360)
                     /locus_tag="VG274_10105"
     CDS             complement(4551..5360)
                     /locus_tag="VG274_10105"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWE07051.1"
                     /translation="MALLDDDACAKLFAMGKSGAGGNIHPGEFFEHVIAEIYTSILNP
                     GDVTLDGGAHVGRHTFPMAEKVGDQGLVLAVEPLPDLARKLAKKAKHRKLGQIRIVGK
                     ALYDRVARIPFHWVKAHSAYSGIERRRYDFKDKIRVVEVEATTIDAILLPGGHGREAP
                     FRFCKLDLEGGEIRALEGARATLVASRPLIIFENDQELSARYYRYTKDEWFGFFKSVG
                     YDVFTLWGRPYRPEDWGRRDIPWYFIAAAGGSDDKAFVTRQLPRLLRHFLR"
     gene            complement(5377..6336)
                     /locus_tag="VG274_10110"
     CDS             complement(5377..6336)
                     /locus_tag="VG274_10110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HWE07052.1"
                     /translation="MHAYLDFERPIAELEGKIVELRQLAAQDPAMDIDDEVARLQARA
                     ESLINDTYAKLTPWQKALVARHPARPHFSDYVSALVEDFTPLAGDRLYSEDRAIISGL
                     GRLRGRVVAIVGQEKGSDTKSRLTHNFGMAMPEGYRKAQRLFSLAERFKLPVVSFADT
                     PGAYPGVGAEERGQAEAIARSTDASLSLGVPFVTMIIGEGMSGGALGIAVANRVYMLE
                     HAIYAVISPEGCASILWRSADKAQEAAAAMRITAQDLLDLKVIDGIVREPPGGAHRAP
                     LQSINAVGDTIARALSEFDELTPAQLRQERRDKYLAIGRSLNA"
     gene            complement(6465..>6869)
                     /locus_tag="VG274_10115"
     CDS             complement(6465..>6869)
                     /locus_tag="VG274_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWE07053.1"
                     /translation="AGAARERHWNDLWHYPLFRLAQAARAHGLIPIDGPFGDYSDAEG
                     FRVQAMRTAVLGCVGKWAIHPSQVALANEVFTPPAGEVARAEAILAALEDAHARGLGA
                     TALDGVLIDAASIRQAHIIVGQMQMIRELESS"
BASE COUNT         1372 a         2152 c         2107 g         1238 t
ORIGIN      
        1 cgcgcaattc ccgaatgagc gcatcctccg gcctctccgc cggctccacc ttgccgcccg
       61 gaaattccca tagcctcgcc atctccttcg cgaccggccg ctgagcgatc aggacccggc
      121 gatcggcatc gatgagcgcg caggctgcaa cgaggacaag ccctttcggc acctttcgat
      181 gctcgcctat cacagattgt gccgtgttaa ggcgccgcca aaggatgctc tagctacggt
      241 aagaagcgtt gacacggatg tacccctcgg tcagatcgca ggtccatacc cgggcgcgtc
      301 ccgctcctac tccaagatcg acgccaatcg cgacttcgcg gccggagact gctcgactcg
      361 caagcccttc gtcgtaggaa gcggcgcgct gtccgctttc tgcgaccagg tatccacaga
      421 aatcaatgcg caaccgatcg cggtccgcgg gttcgcccgc cttgccgacc gccatgacca
      481 cgcgccccca attggcgtcg cccccggcga tggcggtctt caccagaggc gaattggcga
      541 tcgcaaaccc cacgcgccgt gcggattcgt aggtctctgc gccgctcacg tcgatgcgga
      601 tcagcttttg cgcaccttcg ccgtcacgaa cgacctgaag cgcaagctca agcagaaggt
      661 cgtcaagttt ctggcggaac ccccccaaac gtcgatcacc cgcttttgcg atttgcggat
      721 ggggggcacc ttttcccgtg gcaaagacga gaacggtgtc gtttgtcgag gtatcgccat
      781 cgacggtaat cgcgttgaac gaattccgca cgcccgacga aagaagttcc tggagcacag
      841 gcgcggaaag gttcgcgtcc gtgaacacaa aagcgaggag cgtcgccatg tcgggcgcga
      901 tcatgccgga gcccttggcg atgccgttga tggtcacgcg gtgatcttcg atcattgcac
      961 gtgctgtcgc catcttggga aacgtgtcgg tcgtcatgat ggcttccgcc gcggcacgcc
     1021 agccgcctgc cgcaccggca tcggcgaggg tggccatgat ttttgtgatt ttgtgcgagg
     1081 gcaacggttc cccaataacc ccggtcgacg ccacgaacac ctcgtttgcg gggcagtcga
     1141 gcgcggcagc ggccgaagcc gccacttcgc gtaccgcgtc ccggccggcg gcgccggtga
     1201 atgcattggc atttccgcta ttggcgacga gcgcgcgggc tgttcgtttc cccagattgg
     1261 ccctgcacca ttcgactgaa gctgacgccg ttttcgaccg cgtgaaaacg cccgctgcct
     1321 gcgtgccggg cgccaggact gcaagcagca cgtcatcgcg gtcccggtaa cctattcccg
     1381 ctgctatcgt ggccaggcgt acgccagcga gcggaggaag cgcggccagg cttttggggg
     1441 cgagcggcga cactggcgta gccgccgcct ttgttgaacc ggacttcgat gcaaccagtc
     1501 gggtgaaggg gcggacagcc gatcgtgccc gccggctcgg tggcgcggcg cgcgtcttgg
     1561 ccatggaatg ctccccttcc ccatcgctca cgccgcatcg gcggcgttgg gcgcacgcgg
     1621 atttggcccg tttcccttga attgacaagg gggaagggtc ttcttatctc tgccgtcgcg
     1681 cggccggcgc aatccgtgtg accggcggta ttgctgcctt tggcgggttt tcccgcaaaa
     1741 ggctcgcccg gccgtccggc ctcacgagga tatccatgct cggcctcaac gagaatgcgc
     1801 agcgattcct gggcggcatt gggcaacgat tcttcggctc ttccaacgag cggaagttgc
     1861 gcccgatgca gaggcgcgtc caggacatca acgcgcttga gccggctttc gaggccaaat
     1921 ccgacgacga ccttcgcggc ttgacggcca ccttcaaggc gcggctggaa gcaggcgaat
     1981 cgctcgacga tcttttgccc gaagccttcg caacggtccg agaagcggcc aaacgaacgc
     2041 tcggacagcg gcatttcgac gtgcaactcg tgggtggcat ggtgctgaac gacggtcgca
     2101 tcgccgaaat gaagactggc gaaggcaaga cgctcgtcgc cacactgccc gtctacctca
     2161 atgctctcgc cggcgaaggc gtgcatgtag tgaccgtgaa cgactacctc gcgcggcgcg
     2221 atgccgagtg gatgggccag atatacaaat tcctcgggct gaaggttgga tgcatcgtgc
     2281 acggcctcac cgacgagcag cgccatgagg cctatgcctg cgacgtgacc tacggcacca
     2341 acaacgaatt tggtttcgat tatctgcgcg acaacatgaa atacagcatc gcgtcgatgg
     2401 tgcagcgtgg gcaccacttc gcaattgtcg acgaagtcga ctccattctg gtcgacgagg
     2461 cacgcacgcc tctcatcatc tccggaccga cggatgacca gaccgagatt tatcgcgccg
     2521 tggacgtgct gatcccgaaa ctggaaaaga acgacttcga actggacgaa aagcagcgca
     2581 ccgttacgct gaccgaagcc ggcaacgagc ggatggtgga actgctgcgc gaggccaacc
     2641 tgctggagac cggcgatctc tacgacatcg aaaacatcag catggtgcat cacgtcaatc
     2701 aggcgctgaa ggcccacaag atgttccaga gggatcgcga ctacatcgtc aaggacggca
     2761 aggtcgtgat catcgacgaa ttcaccggac gcatgatgga aggccgccgc tattcggaag
     2821 gactgcacca ggctcttgaa gccaaagaaa acacggctat cgaatccgaa aatgtgaccc
     2881 tcgcctcgat cacgttccag aactacttcc ggctctacga aaagcttgcg ggtatgaccg
     2941 gaacggcaat gaccgaagca gccgagttca tggacatcta caagctcgac gtgctcgaag
     3001 ttccgacgaa catgcccgtc aaccggaccg actccgacga cgaggtctat cgcaccgcag
     3061 gcgagaaaga cgaggcgatc attgcgctcg tcaaggactg ccacgaacgg ggtcagccca
     3121 tgctggtggg aacgacgtcg attgaaaagt ccgagcacct ttccgctctg ctcaagaaac
     3181 gcaagatccg gcataacgtg ctgaatgcgc gctaccacga gcaggaggcg gttatcgtgg
     3241 cgcaggccgg cgtatccgga acggtcacca tcgccaccaa catggccggc cgcggcaccg
     3301 acatccagct cggcggcaat gccgacatgc gcattcgcac cgagcttggc ggcgtggtcg
     3361 acgaggcgga acgcacgagg cgcatcgaag aaatcagggc ggagatcgcg accgacaagg
     3421 agcgggcgat cggagcgggc gggctttacg tggtcggcac cgaacggcac gagagccggc
     3481 gcatcgacaa ccagctacgc ggccgctcgg ggcgccaggg cgaccccggc gcatccaagt
     3541 tcttcctgag cctgcaggac gatctcatgc ggatttttgg ctcggaccgg ctggactcga
     3601 tgctgcagcg gctgggactc gaagagggcg aagcgatcgc ccatccctgg gtcaacaagg
     3661 cgctcgaaaa agcgcagcag aaggtcgaag cgcgcaattt cgaaatgcgc aagaacatcc
     3721 tcaaatacga caacgtcctc aacgaccagc ggcgcgtcgt cttcgagcag cggcgcgaga
     3781 tcatgtcggc cgacgatgtg agcgatcaga tcgcggacat gcgcgagcag atcgtggacg
     3841 atcttgtcgc gcgccacatc ccgccgaaag cctatgccga gcaatgggac gcaatcggtc
     3901 tgcaggcgga aatcgagaag atttttgcga tcactcttcc ggtcatcgag tggaccaagg
     3961 aagagggaat cgctgacgag gaagtgcgcg aacgcatgct gaaggcggtc gaatcgaggg
     4021 cggcggagcg cgccgccaat gtcggccccg acatcatgcg ttatgtcgaa aaatctgtcc
     4081 tcctgcagac gcttgaccat gattggcgcg agcacatcgt caatctcgat catttgcgcc
     4141 agtatgtcgg cctgcgcggc tacgggcagc gcgatccgct caacgagtac aagagcgaag
     4201 cgttcgcgct gttcgagggc ttgctgagca agctgcgcag cgacgtcgtg cggcagctca
     4261 tgcatctgca gattaccgtc gagcagcctc ccccgcccat cgagcgcgcg ctgccgccca
     4321 tgcgcgcgca gcacatcgat ccgttgaccg gcgaggacga gttcgcgccc ggacttgtgc
     4381 ccgccgacca ggcagacctg cgcgcgacgc gaatacgcga cgtgccggtc gatcctaacg
     4441 atccgggcac gtggggcaag gtgcagcgca atgccccttg tccgtgcggc tcaggccgca
     4501 aattcaagca ctgtcacggg gcgctggtct gagttgcgat tcaagcgcct tcaacgaaga
     4561 aaatgacgga gcaggcgcgg cagttgtctg gtgacgaacg ccttatcgtc cgacccgccg
     4621 gctgctgcga tgaagtacca gggaatgtct ctgcggcccc aatcttccgg ccggtacggg
     4681 cggccccata gcgtgaacac gtcgtagccg acggatttga aaaacccgaa ccattcgtct
     4741 tttgtatagc ggtagtagcg agccgatagc tcctggtcgt tttcgaatat gatgaggggg
     4801 cgcgatgcaa cgagggtggc acgggcgccc tccaaagcgc ggatctctcc accctcgaga
     4861 tcgagtttgc agaatcggaa tggcgcttcc cgaccatggc cgccgggcaa caaaattgcg
     4921 tcgattgtcg tggcctccac ctccacgaca cggattttat ccttgaaatc gtatcgccgc
     4981 cgttcgatcc cgctatacgc ggaatgggcc ttcacccaat ggaatggaat gcgggcgacc
     5041 ctgtcgtata gcgccttgcc cacaattcga atctgtccga gttttcgatg tttcgccttc
     5101 ttcgccagct tgcgcgcgag atcggggaga ggctccacag ccaggacgag accttggtcg
     5161 ccaaccttct ccgccattgg gaaagtatgg cggccgacat gcgcgccacc gtcgagcgtt
     5221 acgtcgccgg gattcaggat actcgtgtag atttcggcaa tgacatgttc gaagaattcg
     5281 cccggatgga tatttccgcc tgccccactt ttacccatcg cgaaaagctt cgcgcaggca
     5341 tcgtcgtcaa ggagcgccat atgcgcacta taaccattag gcgttcaggc tgcgacctat
     5401 ggcgaggtat ttatcgcgcc gttcctgccg cagttgcgcc ggcgtgagtt cgtcgaattc
     5461 ggacaaggcg cgcgcgatcg tgtcgcccac ggcgttgatc gactggagag gagcacgatg
     5521 cgccccgcct ggcggttcgc gcacgatacc gtcgatcact ttcagatcca gcaaatcctg
     5581 ggccgtgatg cgcatcgccg cggccgcctc ctgcgccttg tcggcgctgc gccacaatat
     5641 cgatgcgcag ccttcaggcg aaatcaccgc gtatatggcg tgctcgagca tgtaaacccg
     5701 gttggcgacc gctatgccga gcgcccctcc cgacatgccc tcgccgatga tcatcgttac
     5761 gaacggcacg ccgagcgaaa ggctggcatc cgtcgaacgc gcgatggcct ccgcctggcc
     5821 ccgctcttcg gcgcccacgc cgggataggc gcccggcgtg tcggcgaacg aaactaccgg
     5881 cagtttgaac cgctcggcga gagagaagag ccgctgcgct ttgcggtagc cttccggcat
     5941 cgccatgcca aaattatgcg taagacggct cttggtgtcg ctgcccttct cttggcccac
     6001 aattgcgaca acccggccgc gcaaccggcc aagaccagaa ataatggcgc gatcttccga
     6061 ataaagccga tcgccggcca gtggcgtgaa atcttcgaca agcgcggaca cgtagtcgga
     6121 gaagtgcggt ctggcgggat ggcgagccac gagcgccttt tgccagggcg tcagttttgc
     6181 gtaggtgtca ttgatcaggg actccgccct tgcctgcagg cgtgccactt cgtcgtcgat
     6241 atccatcgcg ggatcctgcg ccgcaagctg tcgcagctcc acgattttac cttcgagctc
     6301 ggcgatggga cgttcgaaat ccagataagc gtgcattgct tctcattgtt ggcgcccgcg
     6361 gaaacgtggc aagcggcccc cggcctgtca acaaacagcg cccggtaata gaactgcggc
     6421 gtaagttaac aattgccgaa tggacgaacc acggcgcgag ttgctcagct gctctcgagt
     6481 tcacgtatca tctgcatctg cccgacgatg atatgggcct ggcgtataga ggccgcatcg
     6541 atcaagactc cgtcgagtgc agtggcgccc agccctctgg catgggcatc ctcgagcgcg
     6601 gcgaggatgg cttccgcgcg cgcgacctct ccggccggcg gcgtgaacac ctcgttggcg
     6661 agtgcgacct ggcttgggtg aattgcccat ttgcccacgc atcccaaaac tgcagtgcgc
     6721 atcgcctgaa cgcgaaagcc ttcggcgtcg gaatagtcgc cgaacgggcc gtcgatcggg
     6781 atgagaccgt gcgcgcgggc ggcctgcgcc agacggaaca gcggatagtg ccacaggtcg
     6841 ttccagtggc gttcgcgcgc ggcgccggc
//
LOCUS       DASZMC010000490         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2679995,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000490 DASZMC010000000
VERSION     DASZMC010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679995"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VG274_10120"
     CDS             complement(<1..399)
                     /locus_tag="VG274_10120"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HWE07054.1"
                     /translation="MKRLLLLFLFFSSAAFAAPAPESLPNPAQEAHARALQKEFRCLV
                     CQGESIDESNAPLAADLRRLIREHVARGESDAQIRDYLVSRYGQFILMDPPVEPATYA
                     LWAAPFLVLLLGGVTAAFIVFRARNRDAAGG"
     gene            complement(396..2321)
                     /locus_tag="VG274_10125"
     CDS             complement(396..2321)
                     /locus_tag="VG274_10125"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HWE07055.1"
                     /translation="MTGEIGQLALCLALALSLVQSAAGILGVRSAQSRAIASSSALGM
                     FVFVGLAFAALEYAFVTSDFSVADVANNSHTLKPLIYKITGVWGNHEGSMLLWVLVLS
                     VYSAAMALFRRGGDELTSAALGVQGLLAASFILFILLTSNPFLRLDPMPFEGAGLNPL
                     LQDPGLAFHPPVLYTGYVGLSATFAYAAAALITGDIDRTWARAARPFMLIAWIALTVG
                     IAGGSWWAYYDLGWGGFWFWDPVENASLMPWLVGTALLHSALATERTGAFRTWTLLLS
                     IMAFSLSLIGTFLVRSGVITSVHAFASDPKRGVFILLILSLAIGGALALFAWRAPKLA
                     SGAAFEPVSRETTLLINNVFLVTALAVVFIGTLYPILLSALGTKISVGAPYYELFFAP
                     IFLGLMIVLPFGPRLNWRHGDFKGALRTLYPAIGAAAVAAIAVLALVSPRTVAGAGAF
                     AVAAWVIGASVIDVIRIARGNTISIAAFASALAHAGLGITLMGIAGTTLWRAEALEVL
                     GPGETMHIAGYDLTLAHVGAVNGPNYQAIRATLDVRSGGALLGQLAPERRMFPAEGQE
                     TVQTAIRTTGFSDLYLALGDDRGSGRWTIRAYVNPLAPFIWFGAGIMALGGLASLWGR
                     LRSMFFAKSSAPAIEAA"
     gene            complement(2318..2758)
                     /gene="ccmE"
                     /locus_tag="VG274_10130"
     CDS             complement(2318..2758)
                     /gene="ccmE"
                     /locus_tag="VG274_10130"
                     /inference="COORDINATES: protein motif:HMM:NF009731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HWE07056.1"
                     /translation="MTPKKRRRLAFAIALVVAGAGAAALIVFALKDNVLYFYSPSDVQ
                     TKHIASGVAFRIGGLVEKSSVHHGGGAAVDFVVTDGKARVPVEFSGVLPALFREGQGV
                     VAMGRLNDARVFDASEVLAKHDERYMPPEVVDALKRSGHWKEGQ"
BASE COUNT          624 a          933 c          879 g          421 t
ORIGIN      
        1 tccgccggcc gcatcccggt ttcgggcgcg aaatacgatg aacgcggcag taacgcctcc
       61 aagaagcaga accagaaacg gcgcggccca gagcgcgtag gtcgccggtt cgaccggcgg
      121 gtccatcagg atgaactggc cgtagcgcga tacgagataa tcgcggatct gcgcgtcgga
      181 ctcgccacgg gcaacatgtt cgcggatcag gcggcgcaga tcggcagcga gcggtgcgtt
      241 ggactcgtcg atcgactcgc cctggcagac gagacagcga aattcctttt gcagcgcgcg
      301 ggcatgcgct tcctgcgccg ggttcggaag actctccggc gcgggtgccg cgaacgcggc
      361 actggagaaa aacagaaaca aaagcagaag ccgtttcatg ccgcttcgat cgctggcgct
      421 gaactctttg cgaagaacat cgaacgcaac cggccccaaa gactcgcgag tccgccaagc
      481 gccatgatgc cggcgccaaa ccagatgaac ggcgcgagcg gatttacata agcgcggatc
      541 gtccatcgtc cactgccacg atcgtcgccg agcgcgagat agagatcgga gaaacccgtc
      601 gtgcggatcg cggtctgcac cgtctcctga ccttcggcag gaaacatccg ccgctccggc
      661 gcgagttggc ctagcaatgc gccgccactg cgcacgtcga gcgtcgcacg gatagcctga
      721 tagttcggtc cattgacggc gccgacatgc gcaagcgtaa ggtcgtagcc tgcgatatgc
      781 atcgtctcgc ccggtccaag cacttcgaga gcctccgcgc gccacaatgt cgtgcccgcg
      841 atgcccatca gcgtaatgcc gagaccggca tgtgccagcg ccgaagcgaa ggcggctata
      901 gagatcgtgt ttccccgcgc aattcggatc acgtcgatga cgctggcgcc aatgacccag
      961 gcggcaacgg caaacgcccc tgcgcctgcg accgtccgcg gagaaacgag cgcgagaacg
     1021 gcaatcgcgg cgacggcggc ggctccgatc gccggatata aagtccgcaa cgcgcctttg
     1081 aaatcgccat gccgccagtt cagtcgcggg ccgaacggca acacgatcat cagtccgaga
     1141 aagatcggcg cgaagaacaa ctcgtaatac ggcgcgccga cggaaatctt cgtgccgagc
     1201 gccgacagca ggatcggata gagcgtaccg atgaacacca ccgcgagcgc ggtgaccaga
     1261 aacacattgt tgataagcag cgtcgtttcg cggctgaccg gctcgaacgc cgcgccgctc
     1321 gcaagcttcg gtgcccgcca ggcgaacaat gccagcgcgc cgccgatggc gagagagagg
     1381 atcaacagaa tgaaaacacc acgtttcgga tcgctggcaa acgcgtgtac cgacgtgatg
     1441 acgccggagc gaaccaggaa cgtgccgatc aggctcagcg aaaacgccat gatcgagaga
     1501 agcaaagtcc atgtccggaa cgcgccggtg cgttcggtcg cgagcgcgga atgcaacaac
     1561 gcggtgccga cgagccacgg catcagcgac gcgttctcga ccggatccca gaaccagaag
     1621 ccgccccagc cgagatcgta ataggcccac cacgacccac cggcgatgcc gacggtcagc
     1681 gcgatccagg cgatcagcat gaacggccgc gcagcacgcg cccacgtgcg gtcgatgtcg
     1741 ccggtgatca gcgccgctgc ggcgtaagca aatgtcgccg acagcccgac atagccggtg
     1801 tagagaaccg gcggatggaa cgcgagaccg ggatcctgca acaacggatt gagaccagcg
     1861 ccttcaaacg gcatcggatc gaggcgcagg aacgggttgg acgtcagaag aataaaaaga
     1921 atgaacgacg ccgcgagcaa tccctgcacg ccgagcgccg ccgatgtcag ctcatctccg
     1981 ccgcgccgga acaacgccat cgccgccgaa tacaccgaca gcaccaacac ccacagcaac
     2041 atcgagcctt cgtggtttcc ccacacgccc gtgatcttgt agatcagcgg tttcagcgtg
     2101 tgcgaattgt tggcgacgtc ggcgacacta aaatccgatg tgacgaacgc gtactcgagc
     2161 gccgcaaacg ccagccccac gaacacgaac atgcctagcg cactgctcga cgcgatggcg
     2221 cgcgattgcg ccgagcggac gccaagtatc cctgcggccg actgcacgag cgacagcgcc
     2281 agcgcgagac acagcgcgag ttgaccgatc tcgcctgtca ttggccttct ttccagtggc
     2341 cgctgcgctt cagcgcatcg acgacttcgg gtggcatgta gcgctcgtca tgcttggcga
     2401 ggacctcgct cgcatcgaac acgcgcgcgt cgttcagacg tcccatcgcg acgacgccct
     2461 gcccttcgcg gaacagcgcc ggcagcacgc cggagaattc gaccggcacg cgcgccttgc
     2521 cgtcggtcac gacgaaatcg acggccgcgc cgccgccatg atgcacactg ctcttctcga
     2581 ccagcccgcc gatccggaac gcgacgccgg aggctatatg cttcgtctga acgtcgctgg
     2641 gactgtagaa gtaaagaacg ttgtccttga gcgcgaaaac gatcagcgcg gccgcgcccg
     2701 cgccggccac gaccagggca atcgcgaagg caagtcggcg gcgttttttc ggtgtcacgc
     2761 cggttcgtat atataaggtg gggcactctt ccaagattcc gccccggttc cgccggaatc
     2821 gggtccgatg ctcgcgtcat tgctcagggg gatttcc
//
LOCUS       DASZMC010000491         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4876499,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000491 DASZMC010000000
VERSION     DASZMC010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4876499"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /gene="rsmI"
                     /locus_tag="VG274_10135"
     CDS             complement(<1..666)
                     /gene="rsmI"
                     /locus_tag="VG274_10135"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWE07057.1"
                     /translation="MGKPTPSRPKSQSGLERGLFVTATPIGNARDITLRALDVLGRCD
                     AILTEDTRVTAKLLAIHAISRPLVAYNDHNAARMRPAILTRLAAGERIALVSDAGTPL
                     ISDPGYKLVREALAAGERVHVVPGASAVLAALVISGLPTDRFLLVGFLPPGSSARRSV
                     LEELKQLRSSLIFFESPRRLSESLRDMCDVLGRRQGAIVRELTKLHEEVCRGALSDLS
                     LRYS"
     gene            845..1978
                     /locus_tag="VG274_10140"
     CDS             845..1978
                     /locus_tag="VG274_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HWE07058.1"
                     /translation="MIRPLPSATQPTHPLLGDRSGFLRLGNMEPGRTPLRIGVLLPFS
                     SGSATTRMLASGMMKAAAMALFDRKNPDLLLISADEGSGGQAAIAGAQTLMAQGAEVI
                     VGPLFAQSVSAVAPLTRDHGIPLISFSTDRGIAGDGVYLLSFQPENEVQRIVRYAAAQ
                     GHNAFAALIPATVYGAHVAQAFQESVKTAAGRVVGMERFTPASGDVAAPAQKIAEAHP
                     DAILIAQGGTLLVDIAPTLAADGAGSQRVQYLGTGLWDDPATLREPSVAGGWFAAPDP
                     EARRNFEARFRAAYGASPQPLASLAYDAVSLVSVLASGTPYHRFTAQALTDPNGFSGV
                     DGIFRFDPDGTSEHGLAVLSIRPAGGGAIILSPAPTTFLGSAS"
     gene            complement(1980..>2919)
                     /locus_tag="VG274_10145"
     CDS             complement(1980..>2919)
                     /locus_tag="VG274_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HWE07059.1"
                     /translation="LMSGHAVDATLDAIAKRWPLAEEVEITLEANPSSADAKRFRDYR
                     MAGVNRLSLGVQALNDRDLKFLGRVHDAEEARNAANLAAANFERVSIDLIYARPDQTI
                     AQWRGELRDAISLGAAHLSLYQLTIEEGTPFAALVRTGAFAPIDDDTAAALFETTQEI
                     TEDAGLAAYEISNHARPGQECRHNLVYWRYGCYAGVGPGAHGRLLFGDQRMATSNERL
                     PERWLARVAQDGHGIAESVSIPQDVAAREQLLMNLRLSEGIDVEAYRTQWNREIDARR
                     IAALANDGLVSLNGPRLAATCKGRLVLNALIAALAD"
BASE COUNT          504 a          919 c          937 g          559 t
ORIGIN      
        1 cgaatacctt aatgataaat cgctcagcgc gccgcggcag acctcctcat gaagtttcgt
       61 caattctcgc acaatcgcgc cctggcggcg gccgagcaca tcgcacatgt cgcgaagaga
      121 ctcggagagg cgtctgggcg attcgaaaaa gatcaggctg gatcgcaatt gcttcagttc
      181 ctccagcacc gagcgccgcg cactgcttcc aggcggaaga aagccgacga gcaggaaacg
      241 atcggtcggc aagcccgaaa tcaccagcgc tgcaaggact gccgatgcgc caggaacgac
      301 atggactctt tcgccggccg cgagggcttc gcgaaccagc ttgtagccgg gatcggagat
      361 gaggggcgtg ccggcgtcgc tgaccagagc gatgcgttcg ccggcggcaa gccgggtcag
      421 gatggccggc cgcatgcgtg ccgcattatg atcgttgtag gctacaagcg gtcgggagat
      481 ggcgtgaatg gcgagtagtt ttgccgtaac cctcgtatct tcggtcagga tcgcatcgca
      541 gcgaccaagg acgtccaaag cacggagggt gatgtcgcgg gcattgccga tgggcgtcgc
      601 cgtaacgaac aatccccgtt ccaggccgct ttgagatttc gggcgcgagg gggtaggttt
      661 gcccattcca gatccttacc tctccgcgag tgagctctcc atggtccggc attcggcggc
      721 tgctccagcc gtttcacttt cccgcccgct gtggcgattg tgcgcgattg gatcgatcgt
      781 tgcgatggcg gcgatttggg gttgcgcacc gcctccgtca ccgaagccgg ttccgccagc
      841 cccggtgatt cggccgcttc cctccgcgac gcaacccacc catccgctgt tgggcgatcg
      901 ctccggcttc cttcggcttg gcaacatgga gccgggacgc acgcctctga gaattggcgt
      961 cctgctgccc ttttcgagcg ggtcggccac aacccgcatg cttgcttcgg gcatgatgaa
     1021 ggcggcggcg atggcgctct tcgacagaaa gaatcccgac ctgctactca tttccgccga
     1081 cgagggttcg ggcggccagg cggcgatcgc gggagcgcag acgcttatgg cgcaaggcgc
     1141 cgaggtgatc gtgggccctc tgttcgcgca atccgtttct gccgtggcgc cactgacccg
     1201 cgatcatggc atcccgctca tatccttctc gacagaccga ggaattgcgg gagacggcgt
     1261 gtatctcctg agctttcaac cggagaacga ggtccagcga attgttcgct acgcggccgc
     1321 acagggccac aatgcttttg cagcgctgat ccccgccacc gtctacggtg cgcatgtggc
     1381 gcaggcattt caggagagcg tcaagaccgc agccggccgt gtcgtcggca tggaacgctt
     1441 caccccggcg agtggcgacg tcgccgcgcc ggcacaaaaa atcgccgagg ctcatccgga
     1501 cgcgatcttg attgcgcagg gcggcacgct gcttgtggat atcgcgccaa cgcttgcggc
     1561 cgacggggcg ggaagccagc gcgtgcaata tctgggcacg gggctctggg acgatccggc
     1621 aaccttgcgc gaaccgagtg tggcgggcgg ctggtttgcg gcgccagatc cagaggccag
     1681 gcgcaatttc gaggcgcgat tccgcgccgc atacggcgcc agtccgcagc ctcttgcgtc
     1741 gctcgcctat gatgcggttt ctctggtttc cgttctcgcg tccggcacgc cctatcaccg
     1801 attcaccgcg caggcgctga ccgatccaaa cggattttcc ggcgtcgatg gcatttttcg
     1861 gttcgatccg gacggcacgt ccgagcacgg gctcgccgtg ctgagcatcc gccccgccgg
     1921 cgggggtgcg attatcctca gtcccgcgcc gaccaccttt ctgggatcgg cgtcgtaagt
     1981 cagtccgcga gtgcggcaat caacgcgttg aggacgaggc gccccttgca ggttgccgcg
     2041 aggcgcggac cgttcagcga tacaaggccg tcattcgcca gggccgcaat tcgccgcgca
     2101 tcgatttcgc ggttccattg cgtgcggtaa gcctccacat cgattccttc cgaaagacga
     2161 agattcatca gaagctgttc gcgcgcggca acatcctgtg ggatggaaac gctttcggcg
     2221 atgccgtgac catcttgcgc cacacgggcg agccagcgct ctggcaaccg ctcattcgac
     2281 gtagccatgc gctggtcgcc aaacagcagc ctgccgtgcg ctcccggtcc cacgccggcg
     2341 tagcagccat agcgccagta aacgaggttg tggcggcatt cctgcccggg ccgcgcatga
     2401 tttgaaattt cataggcggc caatcccgcg tcttcggtaa tctcctgcgt ggtctcgaag
     2461 agagcggcgg cggtgtcgtc gtcaatcggc gcgaatgcgc ccgtgcggac aagggccgca
     2521 aagggcgttc cctcttcgat ggtcaattgg tagagcgaga gatgtgcggc gcccagcgag
     2581 atcgcgtcgc gtagctcgcc gcgccattgc gcgatcgttt gatcgggccg ggcgtagatc
     2641 agatcgatcg agacgcgctc gaaatttgcg gccgcgagat tcgcggcatt cctcgcttcc
     2701 tccgcgtcat gaacccgccc gagaaatttg agatcgcggt cgttcaatgc ctggactccg
     2761 agcgagagac gattgacgcc tgccattcgg tagtcgcgga agcgcttggc gtctgcgctg
     2821 gatggattgg cttccagcgt gatttccacc tcctcggcga gcggccagcg ttttgcaatc
     2881 gcgtcgagcg tggcatcgac ggcgtgaccg ctcatgagc
//
LOCUS       DASZMC010000492         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1339101,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000492 DASZMC010000000
VERSION     DASZMC010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339101"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VG274_10150"
     CDS             <1..337
                     /locus_tag="VG274_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWE07060.1"
                     /translation="ALALQLFGNCPHRVSVTTVAREAEVSPKKLIRLFAEEVGMTPKA
                     YLRVARFQQVLERVHAAPRVDWMEVVERHGYYDQPHFIREFREFSGFTPTEYFRLRGP
                     QIQHVPLTV"
     gene            384..788
                     /locus_tag="VG274_10155"
     CDS             384..788
                     /locus_tag="VG274_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE07061.1"
                     /translation="MTEKRPDVSINLIARDAAKVLDFYVAGLGAVEISRWIDPKNGKI
                     GHSEFRIGSQRLFLADEYESMDALGVKSPSALGGTSLNLWIEVADVGAAQERAVKAGA
                     KLLVPVETMAVDEGRRCRIADPAGHVWTLAGP"
     gene            complement(785..>1932)
                     /locus_tag="VG274_10160"
     CDS             complement(785..>1932)
                     /locus_tag="VG274_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWE07062.1"
                     /translation="IEDKYPATQRVELRTLAGRMLRFDITFMPGQTLPPPAKQSRYRS
                     NIDRALDDTIAQQIGDKRHFQTVGAGNDFDIRVEVHDGVLRMIVPRERATVSAPDIFV
                     LWMVGSALILLGVAILFLRNQVRPIERLARAADAFGKGRAVPDFKPYGAIEVRRAAQA
                     FLTMRERLERYLQQRTDMLAGVSHDLKTPLTRIRLQLALLKPSPDIDAMRNDVVQMEH
                     MLNEYLDFARGGAGEQSSPNDLTALARDAAERAVRARMAEPRRLTVDAISPIMIAVKP
                     NALGRCVTNLVDNALKHGRHIFVSFARDEKAVDIIVDDDGPGIPENKREDAFRPFHRL
                     DEGRNLQTGGVGLGLAIARDIARAHGGDLLLDKSPQDGLRAIIRLPV"
BASE COUNT          362 a          592 c          605 g          373 t
ORIGIN      
        1 cgcgcttgca ttgcagctct tcggcaattg tccacatcgc gtgtcggtca cgacagtcgc
       61 gcgtgaagcc gaagtgagtc caaagaaact cattcgcttg tttgccgaag aggtcggcat
      121 gacgcccaaa gcatacttgc gcgtcgcgcg attccagcag gttctggagc gtgttcacgc
      181 cgcaccgcgc gtggactgga tggaagtggt cgagcgtcac ggctattacg accagccgca
      241 ttttatccgt gagttccggg agttctcagg cttcacgccg acggaatatt tccgcctgcg
      301 cggcccgcag atacagcacg tcccactgac ggtatgagaa aaatttacaa tcccatctgt
      361 gcgctctgcg ctatgactca cgcatgaccg aaaaacgccc cgatgtttcc ataaatctga
      421 ttgcacgcga tgctgcgaag gtactggatt tctatgtcgc aggccttggc gctgtagaga
      481 tctcgcgctg gattgatccc aagaacggca agatcgggca ttccgagttc cgtatagggt
      541 cacagcgcct gtttctcgca gacgaatacg agtcgatgga tgcgcttggc gtgaaaagcc
      601 cgtccgcact tggcggaacg tcactcaatc tctggatcga agtcgccgat gtcggggcgg
      661 cacaggaacg cgccgtcaaa gcaggcgcaa agctgctcgt gcccgtcgag acaatggccg
      721 tcgatgaagg ccgccgctgc cggatcgccg atcccgccgg ccacgtgtgg acgctcgctg
      781 ggccttaaac cggcagccgg ataatcgcgc gcaaaccatc ctgcggactc ttgtcgagca
      841 gaaggtctcc gccgtgcgcg cgcgcgatgt cgcgggcgat ggcaaggccc aggcccacgc
      901 cgccggtttg cagattgcgg ccttcgtcga ggcgatggaa ggggcggaag gcgtcctcgc
      961 gcttgttctc cggaatgccc ggtccatcgt cgtccacgat gatgtcgaca gctttttcgt
     1021 cccgtgcgaa cgagacgaag atgtggcggc catgcttcag cgcgttgtcg acgagattgg
     1081 tgacgcagcg cccgagcgcg ttcggcttga cggcgatcat gatcggcgag attgcgtcca
     1141 cggtcaggcg gcgcggctcc gccatgcgcg cccgaacggc acgttccgcc gcatcgcgcg
     1201 ccaatgccgt cagatcgttc ggactcgact gctcgccggc accgccacgc gcgaagtcga
     1261 gatattcgtt cagcatgtgc tccatctgga cgacatcgtt gcgcatggcg tcaatgtcag
     1321 gactgggctt cagcagcgcc agttgcaggc gaatgcgggt aagcggtgtc ttcaggtcat
     1381 ggctgacgcc ggccagcatg tcggtgcgct gctgcagata gcgctccagc ctttcgcgca
     1441 tggtgaggaa cgcttgcgcc gcgcgccgca cctctatcgc gccatagggc ttgaaatccg
     1501 gcacggcgcg gcccttgccg aacgcgtcgg ctgcacgcgc gagacgctcg atcgggcgga
     1561 cctgattgcg caggaacagg atcgcgacgc cgagcagtat cagcgccgag cccaccatcc
     1621 aaagaacgaa gatatccggt gccgaaaccg tggcgcgttc acgcggcacg atcatgcgca
     1681 acacgccgtc atgcacttca acgcgaatat cgaaatcgtt gccggcgccc acagtctgga
     1741 agtgccgttt gtccccgatc tgctgcgcaa tcgtatcgtc gagcgcgcgg tcgatgttgc
     1801 tgcgatagcg ggactgtttg gccggcggcg gaagcgtctg tcccggcatg aaggtgatgt
     1861 cgaagcgtag catgcggccc gcaagcgtgc gcaattccac gcgctgcgtc gccggatatt
     1921 tatcttctat cg
//
LOCUS       DASZMC010000493         5543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4056483,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000493 DASZMC010000000
VERSION     DASZMC010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4056483"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..852
                     /locus_tag="VG274_10165"
     CDS             226..852
                     /locus_tag="VG274_10165"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWE07063.1"
                     /translation="MQGGLESKWRRRKDARPQEILDAALAEFAARGFAAARMDDIAAR
                     AGVTKGTVYLYFPSKEELFKSLVRESIGAAIEARMSDARSFEGDSSELLALVLRAIGY
                     FARTSDRAVLPKIVMAEAGNFPELLRFYRAEVVDKGLSLLGSIVTRGIARGEFRSLEP
                     QHAARLCIAPLLFAVFWRSSFAAQDAVPYDLEGFIETHIDVLLKGLAA"
     gene            917..1789
                     /locus_tag="VG274_10170"
     CDS             917..1789
                     /locus_tag="VG274_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWE07064.1"
                     /translation="MRWGIRIAGLLVLAALAGFVWWFTHRERPPQELTLYGNVDLREV
                     DLAFNNNERVLKVLVAEGDRVHRGQVLARVDTSRLAPQVAEAEANVALDGVNLANARD
                     QYQRNLTLSRNSGGRGVARQDVDNTKAAFDAQAARRQADAAQLALLRQELADAVLVSP
                     ADATVRTRIMEPGEMASPTRPVFSLALTDPKWVRVYVSETDLGKLRPGMAATISVDAF
                     PGKRFPGWIGFISPTAEFTPKSVETEDLRTSLVYEVRVFVKDPADALRLGMPATAHVK
                     LARFAAEARPDSRP"
     gene            1786..3528
                     /locus_tag="VG274_10175"
     CDS             1786..3528
                     /locus_tag="VG274_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWE07065.1"
                     /translation="MSALALRADAIRKTFRRGPKETVVALDGVSFTAQTGSLSALVGP
                     DGAGKTTLLRLATGLLSADSGAMEVLGIDAARAPQDIQNRIGYMPQRFGLYEDLTVQE
                     NLDLYADLHGVSRATRPGRYRRLMEMTALGPFTRRLAGQLSGGMKQKLGLACALVRSP
                     EMLLLDEPTAGVDPLSRQELWEIIGELVADRQTTVILCTSYLDEAERCGHVVVLHEGR
                     VLAQGAPHEVSGRAEGCVFLADPPAGQTARGLQARLLDSVDIVDADPDAGRVRFVLKH
                     NASAPRGVEATPVEPRFEDGFMTLLQHKRESGASASFTLERPLKDHTTEAVEVRDLVR
                     MFGSFTAVDHVSFDVRRGEIFGLLGPNGAGKTTTFRMLCGLLPASSGTLKVAGLDLRT
                     ARASARQRIGYVAQKFSLYGDLTVRENLDFFASAYGLRGEARRRRTRWALEQFELGSH
                     VDLRSAELPGGYKQRLAMAAALLHEPEILFLDEPTSGTDPLARREFWRRITALGEQGV
                     TVIVTTHFMEEAEYCDHVAILDGGRVLAQGTPAEIRTHVPTEAGRAPTMENAFIAIVE
                     KARREESARRRSAA"
     gene            3525..4658
                     /locus_tag="VG274_10180"
     CDS             3525..4658
                     /locus_tag="VG274_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWE07066.1"
                     /translation="MTRDDFTAKLRRVLALMRKEGFQIVRDPSSIALGVVMPVMLILL
                     FGYGLSLDVKNVPVAVVIENPAPLATEAAAGFRLSPYFDAHIMTSMKDAEKLMLERKV
                     DGIVRLRSNFSRDLSLGDAQAQILVHGADANRARIIEAYAQGALAQWAARQQAQGESV
                     TGGPVGVVNRLWFNAANDSHYFLVPGLIVLIITLIGAFLTTMVVAREWERGTLEALFV
                     TPMRTDEFLLGKTLPYFLLGMIGLGLCFLASRFMFGVPFRGSIWLFAGISTLYLMIAL
                     GIGLLISSAVKSQFVASQLTMVVTFLPAMMLSGFIFDLRSMPAAVRAITYVLPARYFV
                     ALLQTVFLAGNVWSVVLPDTAILAAMVVMLLVLSRRATRKKLA"
     gene            4667..>5543
                     /locus_tag="VG274_10185"
     CDS             4667..>5543
                     /locus_tag="VG274_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWE07067.1"
                     /translation="MRESLARIFALIRKELLAMLKDPRARWSIVVPPVLQCLIFGYAA
                     TYDLNNVSYAVLDQDRSIASHDLLARFDGSRVFQRVANLERAADIAGVIDNRDAILVI
                     QVPQDFERDLLLGKPANVQLIADGRNSNTAGVAQAYAGAVVDAFNADWRSQHGEAGPP
                     IDVASRAWFNSNLETRWNMIPSLIGTITLIQTMMLAAMSIAREREQGTFDQLLVTPFR
                     PLEIMAGKAVPSMIIGTIQATNILLVAQLWFRIPFQGSFVVLYAGLVLFLLAAVGIGI
                     FVSSIAATMQQAMLYS"
BASE COUNT          902 a         1813 c         1832 g          996 t
ORIGIN      
        1 tgcctcaacc gcagcatctc ccctcagtga ggaacaatct cccgccactt tctttcctcc
       61 tccgtagctc gccgcggctc gcgcgcgcgg cgcggcggga ggggcaggtg cccgccccat
      121 ggtccgcgca aagcgcggcc ggaggataaa ctccgcggga ggaggccgga gtgggggcac
      181 cttttgaggt attgtaaata actcaccggt tagttacggt tcggcatgca ggggggcctc
      241 gaatcgaaat ggcggcggcg gaaggacgcc cggccgcagg aaattctcga tgcggcgctg
      301 gccgagttcg ccgcgcgcgg gtttgccgcc gcccggatgg acgacatcgc ggcgcgcgcc
      361 ggggtgacca agggcaccgt ctatctctac tttcccagca aggaggagct gttcaaaagc
      421 ctggtgcgcg agtcgatcgg tgcggcgatc gaagcccgca tgtcggatgc gagaagtttc
      481 gaaggcgact cctccgagct tctcgcgctg gttctgcgcg ccatcggata tttcgcccgc
      541 accagcgacc gcgccgtgct gcccaagatc gtcatggcgg aggcggggaa ttttcctgag
      601 ctcctgcgct tctaccgcgc cgaggtggtc gacaaggggc tttcgcttct cggttccatc
      661 gtcacgcgcg gcatcgcgcg cggcgaattc cgcagcctgg agccgcagca cgcggcccgg
      721 ctgtgcattg cgccgctgct gttcgcggtc ttctggcgct cgagtttcgc cgcgcaggac
      781 gctgttccct acgatctcga aggcttcatc gagacgcata tcgacgttct gctcaaaggg
      841 ctcgccgcat gaacgataag ggagacatga tgacgggaag gggcgctgaa acaacgcgac
      901 cgcggggcgg ccgccgatga gatggggaat ccgcatcgcg ggtctgctcg tgctcgcggc
      961 gctcgccggc ttcgtctggt ggtttaccca ccgcgaacgg ccgccgcagg agctgaccct
     1021 ctacggcaat gtcgatctcc gggaggtcga tctcgccttc aacaacaatg agcgcgttct
     1081 aaaggtgctg gtggcggagg gtgaccgcgt ccatcgcggc caggttctgg cgcgcgtcga
     1141 tacgagccgg ctggccccgc aggtcgcaga ggcggaagcg aatgtggcgc tcgacggcgt
     1201 caacctcgcc aatgcgcgcg atcaatatca gcgcaacctg accctgtcgc gcaattcggg
     1261 cggacgcggc gtcgcccgcc aggacgtcga caataccaaa gccgccttcg acgcgcaggc
     1321 ggcgcgccgc caggcggatg ccgcccagct ggcgctcctg cgccaggaac tcgccgatgc
     1381 ggttctcgtc tccccggccg acgcgactgt ccgtacacgg atcatggagc cgggcgagat
     1441 ggcgtcgccg acgaggccgg tgttctcgct cgccctcacc gatcccaaat gggtgcgcgt
     1501 ctatgtctcc gagacggatc tgggcaaact gcgtccgggc atggcggcga cgatctcggt
     1561 ggatgcgttt ccgggcaagc gcttcccggg ctggatcggg ttcatttcgc cgaccgcgga
     1621 attcacgccg aaatcggtgg agacggagga tctgcgcacc agcctcgtct acgaggtgcg
     1681 cgtcttcgtc aaggatccgg cagacgcgct tcgcctcggc atgccggcga ccgcacacgt
     1741 gaaactggcg cgcttcgccg ccgaggcgcg gccggattcg cggccatgag cgcgctcgca
     1801 ctccgcgccg atgcgatccg caagacattc cggcgcgggc cgaaggaaac cgtggtggcg
     1861 ctcgacggcg tctccttcac tgcgcaaacg ggatcgctga gcgcgctggt cggtcccgac
     1921 ggcgccggca agaccacgct gcttcgactc gcgacgggac tgctgagtgc ggattcgggt
     1981 gcgatggaag tgctgggcat cgacgccgcg cgcgcgccgc aggacatcca gaaccgcatc
     2041 ggctacatgc cgcagcgctt cggcctttac gaagatttga ccgtgcagga gaacctcgat
     2101 ctctacgccg atctccatgg cgtgagccgc gccacgcggc cgggccgcta caggcggctg
     2161 atggagatga cggcgctggg tcccttcacg cggcgactcg cgggccagct ctccggtggc
     2221 atgaagcaga aactgggcct cgcctgcgcg ctggtgcgct cgcccgagat gctgctcctc
     2281 gacgagccga cggccggcgt cgatccgctg tcgcgccagg aattgtggga gatcatcggc
     2341 gaactcgtcg ccgaccggca gacgacggtc atcctctgca cgtcctatct cgacgaagcc
     2401 gaacgctgcg gccatgtcgt ggtgctgcat gagggcaggg tgcttgcgca gggcgcgccg
     2461 cacgaggtga gcgggcgggc agaggggtgc gtgtttcttg ccgatccgcc ggcaggccag
     2521 acggcgcgcg gccttcaggc gcggcttctc gacagcgtgg acatcgtcga tgcggacccg
     2581 gacgcggggc gcgtgcggtt cgtccttaaa cacaacgctt ccgcaccgcg cggagtcgag
     2641 gccacccccg tcgagccgcg cttcgaagac gggttcatga cgctgctgca gcacaagcgc
     2701 gagagcggtg cgtccgcatc gttcacgctc gaaagaccgc tgaaagatca caccaccgag
     2761 gcggtggagg ttcgcgacct cgtgcgcatg ttcggaagct tcaccgccgt cgaccacgtc
     2821 agcttcgatg tccggcgcgg agaaatcttc ggactgctcg gacccaacgg agcaggaaag
     2881 acaacaacct tccgcatgct gtgcgggctg cttccggcca gcagcggcac gctcaaggtg
     2941 gcaggcctcg atctgcgtac ggcgcgcgct tcggcacgcc agcgcatcgg ttacgtggcg
     3001 cagaaatttt cgctctatgg cgatctgacg gtgcgggaga atctcgattt cttcgccagc
     3061 gcctacggtc tgcgcggcga ggcgaggcgg cgccgcacac gctgggcgct cgagcaattc
     3121 gagctgggct cccatgtcga tctgcggagc gcggaattgc cggggggcta caagcagcgg
     3181 ctggcgatgg ccgcggcact tctccatgag ccggagatac tctttctcga cgagccgacg
     3241 agcggcaccg acccgctcgc ccgccgcgaa ttctggcggc gcatcaccgc actcggcgaa
     3301 cagggcgtca ccgtcatcgt caccacgcat ttcatggaag aggcggaata ttgcgaccac
     3361 gtcgcgattc tcgacggcgg gcgcgtgctc gcgcagggca cgccggccga gatcagaacc
     3421 catgtgccca cggaagcggg gcgggcgccc accatggaga acgccttcat cgccattgtc
     3481 gagaaggcgc gccgtgagga aagcgcgcgg cggcggagcg cggcatgacg cgcgacgatt
     3541 tcacggccaa gcttcgccgc gttctggcgc tgatgcgcaa ggagggtttt cagatcgtgc
     3601 gcgatcccag cagcatcgcg ctcggcgtcg tcatgccggt catgctgatc ctgctcttcg
     3661 gttacggact ttcgctggac gtgaagaatg tgccggtcgc ggtggtgatc gagaatcccg
     3721 cgccgctggc gacggaagcc gcagccggct tccggctgtc gccctatttc gacgcgcaca
     3781 tcatgacgtc gatgaaagac gcggaaaagc tgatgctcga gcgcaaggtg gacggcatcg
     3841 tccggctgcg atcgaatttc agccgggacc tcagcctggg tgacgcccag gcgcagatcc
     3901 tcgtgcatgg cgcagacgcg aaccgcgcgc ggatcatcga ggcctatgcg caaggcgcgc
     3961 tggcacaatg ggcggcgcgg caacaggcgc agggcgagag cgtaaccggc ggaccggtcg
     4021 gcgtcgtcaa ccggctgtgg ttcaatgcgg ccaatgacag tcactatttt ctggtgcccg
     4081 gcctgatcgt tctgatcatc acgctgatcg gcgcatttct caccaccatg gtggtggcgc
     4141 gcgagtggga gaggggaacg ctcgaggcgc tgttcgttac gcccatgcgc accgacgaat
     4201 tcctcctcgg caagacgctg ccctatttcc tgctggggat gatcggcctc ggtctgtgct
     4261 ttctcgcctc gcggttcatg ttcggcgtgc cgttccgcgg ctcgatctgg ctctttgccg
     4321 gcatctcgac gctctatctg atgatcgcgc tcggcatcgg cctgctcatc tcctcggccg
     4381 tgaagagcca gttcgtggcg agccagctca cgatggttgt gacctttctt ccggcgatga
     4441 tgctgtcggg attcatcttc gacctcagaa gcatgccggc ggcggttcgc gccatcacct
     4501 atgtgctgcc cgcccgctat ttcgtcgcgc tgctgcagac ggtgttcctg gccggcaatg
     4561 tgtggagcgt cgttctgccc gacaccgcga tcctggcggc gatggtcgtc atgctcctgg
     4621 tgctgtcgcg gcgcgcgacg cgcaagaagc tcgcctgagc atcgccatgc gggaaagcct
     4681 cgcgcgcatc ttcgctctca tccgcaagga actcctggcg atgctcaagg acccgcgcgc
     4741 ccgctggagc atcgtcgtgc cgccggttct gcaatgcctg atcttcggct acgcggccac
     4801 ctacgatctc aacaacgtgt cctatgccgt gctcgaccag gaccgcagca tcgcttcgca
     4861 cgacctgctg gcgcgcttcg atggttcccg cgtcttccag cgcgtcgcca accttgagcg
     4921 cgccgccgat attgccggcg tcatcgacaa tcgcgacgcg attctggtca tccaggtgcc
     4981 acaggatttc gagcgcgatc ttctgctcgg aaagccggcg aacgtgcaac tgatcgccga
     5041 cggccgcaat tcgaacaccg cgggcgtggc gcaggcctat gcgggcgcgg ttgtggacgc
     5101 tttcaatgcg gactggcgca gccagcacgg cgaggccgga ccgccgatcg atgtcgcgtc
     5161 gcgcgcctgg ttcaattcca atctcgaaac gcgctggaac atgatcccca gcctcatcgg
     5221 cacgattact ctgattcaga ccatgatgct ggcggcgatg tcgatcgcgc gcgaacgcga
     5281 gcagggcacc ttcgaccagc ttctcgtcac cccgttccgg cccctggaaa tcatggccgg
     5341 caaggcggtg ccgtcgatga tcatcggaac aatccaggcg accaacattc ttctcgtggc
     5401 gcagctgtgg ttccgtattc cctttcaggg ctcgttcgtc gtgctctatg ccggcctcgt
     5461 gctgtttctt ctggctgcag tcggcatcgg catcttcgtc tcctcgatcg ccgccaccat
     5521 gcagcaggcg atgctctaca gct
//
LOCUS       DASZMC010000494         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2409316,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000494 DASZMC010000000
VERSION     DASZMC010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409316"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1018
                     /gene="mmsB"
                     /locus_tag="VG274_10190"
     CDS             95..1018
                     /gene="mmsB"
                     /locus_tag="VG274_10190"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HWE07068.1"
                     /translation="MATGNCNCEDLRMTNIAFIGVGNMGGPMARNLVKAGHGVTAFDL
                     SPAVLQPVVEAGARRAASAAEAVREADAVITMLPAGQHVRSVYLDGGILAHARKSAVL
                     IDSSTIDIDSARAVHAAAAEAGVDFLDAPVSGGVGGAEAATLTFMCGGTESAFERARP
                     ILEQMGKRIVLAGGAGAGQTAKMCNNMLLAISMIGTCEAFVLAEKLGLDAQKLFDVAS
                     TSSGQCWSLSAYCPVPGPAPSAPSNRDYQGGFAAALMLKDLNLSQAAAQASGATTPLG
                     AEAAQLYSLFAAKGHGGLDFSGIIRMLRGEL"
     gene            1086..1598
                     /locus_tag="VG274_10195"
     CDS             1086..1598
                     /locus_tag="VG274_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HWE07069.1"
                     /translation="MTAMAKPQLQAAALEGDRSLRKRYLEVLSLIERLHRQALDVIKD
                     ELDRRDEREINSVQALLLFNVGDQELSAGELRSRGHYLGSNVSYNLKKLVESGYVHHE
                     RSVVDRRSVLVRLTRKGEAVRDMLAGMFERHLLSLEAVGNVGAGELETVSTSLRRIER
                     FWIDQVRFQL"
BASE COUNT          311 a          539 c          595 g          336 t
ORIGIN      
        1 gcgacgatca tcgacaagga tggcgcaccg cgctggcggc cggactctct ggcggatgtg
       61 agcgccgccg atgtgacgtc ctgcttcgcg cagcttggcg acagggaact gcaattgtga
      121 ggatctgcgc atgacgaaca tcgctttcat cggtgtcggc aacatgggcg gccccatggc
      181 gcgcaatctc gtgaaagcgg ggcacggcgt gaccgccttc gatctcagtc cggctgttct
      241 gcaaccggtc gtcgaagcag gcgcgcgccg tgcggcttcg gcggcggaag ccgtgcgcga
      301 ggccgatgcg gtgattacca tgcttccggc tggccagcat gtccgcagcg tgtatctcga
      361 cgggggcatt cttgcgcacg cacgcaaaag cgctgttttg atcgacagct ccacgatcga
      421 catcgattcc gcgcgtgcgg ttcacgcggc ggcggcggag gcgggcgtcg attttctgga
      481 tgcgccggtc tccggcggcg tgggcggcgc ggaagccgcc acgctcacct tcatgtgcgg
      541 cggcacggag agcgccttcg agcgcgcccg gccgattctc gaacagatgg gcaaacgcat
      601 cgtgctcgca ggcggcgcgg gggcgggcca gacggcgaag atgtgcaaca atatgctgct
      661 ggccatttcg atgatcggga cctgcgaagc gttcgtgctg gcggagaaac tcgggctcga
      721 cgcgcagaaa ctgttcgacg tcgcatcaac gtcatcgggg cagtgctggt cgctttcggc
      781 ctattgtccg gtcccgggac cggcgccgtc cgcgccgtcc aatcgcgatt accagggcgg
      841 attcgctgcc gcattgatgc tgaaggatct caacctctca caggcggccg cccaggcttc
      901 gggcgccaca acgccgctgg gcgccgaagc cgcgcagctc tattcgctgt tcgctgccaa
      961 gggtcacggc gggctcgatt tttccggaat catccgcatg ttgcgcggcg agctgtgacg
     1021 gtttaccggc ccttaaggtc tcgcgcctag tgtttcgcga aagcgggtcc ggtttggggt
     1081 ttgatatgac ggcgatggca aagccgcagt tgcaggctgc agcgctggaa ggcgatcggt
     1141 ctctgcgcaa gcgctacctt gaagtgctca gcctgatcga gcgccttcat cgccaggcgc
     1201 tggacgtcat caaggacgag ctcgaccgcc gcgacgaacg cgaaatcaac agcgtccagg
     1261 ctctcctgct cttcaacgtc ggcgaccagg aattgagcgc cggcgagctg cgatcgcgcg
     1321 ggcactatct gggctccaac gtgtcctaca atctgaagaa gctcgtcgag tccggctacg
     1381 ttcatcacga acgctccgtc gtcgaccgca ggtctgtcct ggtgcggctg acgcgcaagg
     1441 gcgaagcggt gcgcgacatg ctggccggaa tgttcgagcg ccatctgctg tctctggaag
     1501 cggtcggcaa tgtgggggcg ggcgagcttg aaacggtcag caccagcctc aggcggatcg
     1561 agcgtttctg gatcgaccag gtacggttcc agctctaacg gcaaatctgc gacttcccat
     1621 acgtccgtca tggccgggcc accccggtca gcgtgagcgc gcggccggag tgtaaaccgt
     1681 gccggccatg atatgttcac aggcgtgcgc gccagtcttc agttggaatt tcggatgtcc
     1741 aagcatgtaa tttggccgcg aagaatacag aaaataattt a
//
LOCUS       DASZMC010000495         5176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2953003,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000495 DASZMC010000000
VERSION     DASZMC010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953003"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..2506
                     /locus_tag="VG274_10200"
     CDS             113..2506
                     /locus_tag="VG274_10200"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HWE07070.1"
                     /translation="MTNLSDSTVSRIGWKAERHQRMIALGRLLLAWERAWPALWPASG
                     VLGLYAAAALFGLLDPLSGALRSLLLLGALTAAGYALYRGFQRWKMPSWDDAARRVEC
                     DSRLLHRPITEGRDRLAAGAGDPMAELLWRAHAAQLLRGLGKLRLAPPSPGLAAKDPH
                     ALRYLVLLLVLGGFFTAGADWQRRLSLASSFDQAASVPVASIDAWITPPAYTGWAPLY
                     LRPAGGEVAVPAGSELVMRVHGSGAAPRLALDPAPRGGVPDFGGANGAWGTSVKLAQD
                     TRVIVRSAGHFLGRWRIRAIADQPPVIAFSAAPSRTERDALKIAFTAGDDYGVTGARA
                     VIRPLSRNSRASLAVDLPLPEASAKTVTETIYRDLTAHPFAGLDVEMVLEASDGAGQI
                     GRSQPVHVRLPERIFANPLARALVEQRQTLALGGHDARAAKALDALAFAPQRFYANQP
                     SLYLGLRTAYWTLRVARSAEDIARVQDLLWQMANALEQGGLLDAAERLRELQQLLSQA
                     LARNAPQSEIDALLQRYREALQHYLQALAQNGPRSNGPLPANAKVLRPEDLEAMLKAI
                     QQLAQTGARGKAAEMLSMLQNMLENLHAGSGPGDSGGAGQADKAMSDAIQGLGDMIGR
                     QRQLLDKSLRQGQGAGDPKDGGGKGLSAQQGRLREDLGRIEKGLGQQKLPGGDRLGEA
                     EREMGNAENALGAQAFDQAGQAQKNALEAMKEGAGQLAQELMKKSGQNNGNGQPNEDP
                     LGRAEGAVGGAGSDTKLPNQTEMQRARAILEELRKRAAQPGRPKEELDYIDRLLRQF"
     gene            3580..>5176
                     /locus_tag="VG274_10205"
     CDS             3580..>5176
                     /locus_tag="VG274_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWE07071.1"
                     /translation="MQARSPILIAGVIAAGFARAALAQTAPQQGIETVTVIAVSPLPG
                     TGIDVDKIAGETETLSMSDVRRDSPQSALPDAAAKRLSSVSIADEQGSQFQPDFVYRG
                     FEASPVSGAAEGVAVYQDGVRLNESFGDNVNWDLVPEFAVDRLTVQSNNPVFGLNAIG
                     GAVTLAMKNGLDFEGGDAELGGGSFGNLNGSAEYGARIGKLGIYLGIGGLEDDGFRHH
                     SPTALRQAYGDLAWQNGRLTFHLSASGALDNIGAVGPTPVQLLAQDPRSVFTWPQSTE
                     NGMELVQLRGNWRATDELSLSANAYFRRFLQHIVDGNTTDVVRCANDSSELCLEGNDL
                     YPGDALYDAQGMQVPASVLPPGATPGETDFTRTDTNSAGAAIQASLTAPVLAHANNLT
                     IGASFDRGVSDYAAHGELGTLTENLHVLGSGVIIDQGLSPTAEPPIEEPVNVDATNLY
                     AGFYAIDVFDVTPQLSWTLSARLNLAQIGLQDRLGDALTGDHAFTRLNPGTGLAYKLS
                     DRVTLYGGYSESNRAPTAGELSCA"
BASE COUNT          916 a         1706 c         1703 g          851 t
ORIGIN      
        1 acggcgggcg cctatggcgc ggtaatggct tccgcctaca atgcgcggcc tcccgcctgc
       61 gaggttctgg tgaaagacgc ggcgtggagc attgtgcggc cacggctgag ctatgacgaa
      121 cttgtcggat tcgaccgtct cccgcattgg ctggaaggct gaacggcacc aacgcatgat
      181 cgcgctgggg cgtctcctgc tcgcatggga gcgcgcatgg ccggcgctgt ggcctgcaag
      241 cggcgttctc gggctttatg cggcggccgc cctcttcgga cttctcgatc cgctttccgg
      301 cgcgctgcgc tcgcttcttc ttctgggcgc tctgacggcg gcgggatatg cgctttaccg
      361 cgggttccag cgctggaaaa tgccatcctg ggacgatgcg gcccggcgcg tcgaatgcga
      421 cagccggctg cttcaccgcc cgattaccga aggccgcgac cgccttgcgg cgggcgccgg
      481 agatcccatg gcggagctgc tgtggcgggc gcacgcagca cagctcttgc gcgggctggg
      541 caagcttcgc ctcgccccgc cgtcgccggg gctcgccgcg aaggacccgc atgccctgcg
      601 ttacctggtg ttgctgctcg tgctcggcgg atttttcacc gcgggcgccg actggcagcg
      661 ccggctgtcg ctcgcttcgt ccttcgacca ggccgccagc gttcccgtcg catcgatcga
      721 tgcctggatc acgccccccg cctacacggg atgggcgccg ctctatctgc ggcctgcggg
      781 cggcgaggtt gcggtgcccg cgggctcgga gctggtaatg cgcgtgcacg ggtcgggcgc
      841 cgcgccgcgt ctggccctcg atcccgcgcc gcgtggcggc gtgccggatt tcggcggcgc
      901 gaacggcgcc tggggcacaa gcgtgaagct cgcccaggat acgcgcgtca ttgtccgctc
      961 ggccgggcat ttcctcggac gctggcgcat ccgcgcgatc gccgaccagc cgccggtcat
     1021 cgcgttttcc gcagccccca gccgcaccga gcgcgacgcg ctgaagattg ccttcaccgc
     1081 cggcgacgat tacggcgtca ccggtgcgcg cgcggtcatc cgcccgctgt cgcgcaattc
     1141 gcgcgccagc ctcgcggtcg atctgccgct ccccgaagcc tccgcaaaga cggtcaccga
     1201 aacaatctac cgcgatctca cggcccatcc cttcgccggc ctggatgtcg agatggtgct
     1261 cgaagcgagt gacggggccg ggcagatcgg ccgcagccag cccgtgcatg tgcgcctgcc
     1321 cgaacgcatc ttcgccaatc cgctggcgcg cgcgctggtc gagcagcgcc agacgctggc
     1381 attgggggga catgatgcgc gcgcggcgaa agcgctcgac gcgctcgcct tcgcgccgca
     1441 gcgcttctat gccaatcagc cttccctgta tctcggcttg cgcaccgctt attggactct
     1501 gcgcgtcgcg cgcagcgcgg aggatatcgc gcgcgtgcag gatctgctct ggcagatggc
     1561 gaacgcgctc gaacagggcg gattgctcga cgcggcggag aggctgcgcg agctgcagca
     1621 gcttctgagc caggcgctcg cccgcaacgc cccgcagagc gagatcgacg cgctgctgca
     1681 gcgctaccgc gaggcgctgc agcactatct gcaggcgctg gcgcagaacg gtccgcgttc
     1741 caacggaccc ttgccggcca acgcgaaggt gctgcggccc gaagatctgg aagcgatgct
     1801 gaaggccatc cagcagctcg cgcagacggg cgcgcgcggg aaagccgcgg aaatgctttc
     1861 gatgctgcag aacatgctgg agaatttgca tgcgggatcg ggtccgggcg acagcggggg
     1921 cgcgggccag gccgacaagg cgatgagcga cgccatccag ggactgggcg acatgatcgg
     1981 acgccagcgc caattgctcg acaaatcctt gcgccaaggt cagggcgcgg gcgatccgaa
     2041 ggacggcggc ggcaaaggtc tttccgcgca gcagggccgg ctgcgcgaag atctcggccg
     2101 catcgaaaaa gggctcgggc agcagaagct tcccggcgga gaccggctcg gcgaagccga
     2161 acgcgaaatg ggcaatgccg agaacgcgct gggcgcgcag gctttcgacc aggcgggcca
     2221 ggcgcagaag aacgcgctcg aagcgatgaa ggaaggcgcc ggccaactcg cgcaggagct
     2281 gatgaagaag agcggacaga acaatggaaa cggccaaccc aacgaagatc cgctcggccg
     2341 cgccgaaggc gcggtgggcg gcgccggcag cgacacgaag ctgcccaacc agacagagat
     2401 gcagcgcgcg cgcgccatcc tcgaagagct gcgcaaacgc gccgcccagc ccggccgccc
     2461 gaaggaagaa ctcgactaca tcgacagact gctcaggcag ttttgaccgg cggaggcttg
     2521 ggctcgtttt ctgagcgcgc ccctggaaaa tgcgcctgga cagcccgttc cacccaacgg
     2581 acgaacgtct ccctatgctc ccctcacaag ttcgaatcct gtccggtgcg ccggtgtctt
     2641 cgggcggcag gccgccacga aagaagcgag caaatcgctg ccccgccatt tgcggcaagg
     2701 gccgaacagc gaatttaaca gcgaagatca gctttaacag cgaagatcgg caatccgtgg
     2761 caccgggagg tttcgtcgcg cgggtcaata aaaacccgga ccaccgcttc ggcgccgggt
     2821 gcaacatttc acgatgacga agggccgaac agcgatttta gcagcgaaaa tcgacaatcc
     2881 gcggggccgg gaggtttcgt cgcgccggtc aataaaaagc ccggccaccg ctccggcgcc
     2941 gggtgcaact tttcaccatg ccgcaatcga tacggcgttt gatcaaacac gtcaaggtca
     3001 agtacgtttc acagcgcaat cctctccgta catggaacgt ttgcatcccg cgccggggcg
     3061 attgctttgg gctggccgac cttcagaatc cggcgacagc tcatgaggta ccattacacc
     3121 ggcatgtttg gactgcagcc tcgtgttgac ggaaaacaag aggccgcaaa atctatctca
     3181 tgtcccttga tcggaacaaa gaccaagttg ttcgatccag ttttttgtaa cacgcccgat
     3241 agccctcgca atttccgtat gcgcaagcgc cgccccctcc gcctcgcttt gctcggcacc
     3301 tgctccaagg gaggaaagaa gatgggggag gaaagatatt agcgcggaaa ggtccgcaca
     3361 ttgttttcct cccgcatcgc tcgccgcgct gcgcgcgtgt ctttcctccc ccatcgtggg
     3421 ggaggtggcc cgaagggccg aagggggagt gatacgggga cagtttactt gttatgcggg
     3481 atgcaggctc gcgggacggc ttcacgatcg cgctccctgc ttgcattgca cgggcgcttg
     3541 gtggaaaacg gacgggcgag gcgggggacg cgcgagggca tgcaggcgag gtcgcccatc
     3601 ctgattgcgg gcgtcatcgc ggcgggattt gcgcgcgcgg cgctggcgca gaccgcgccc
     3661 cagcagggca tcgaaacggt cacggtgatt gcggtatcgc ccctgccggg caccggcatc
     3721 gacgtggaca agatcgccgg cgagaccgaa accctctcca tgtccgatgt caggcgcgac
     3781 agcccgcaga gcgcgcttcc cgatgccgcc gccaagaggc tctcgagcgt gagcatcgcc
     3841 gatgagcagg gaagccagtt ccagcccgat ttcgtctatc gcggtttcga agcctcgccg
     3901 gtttcgggcg ccgccgaggg cgtcgccgtc tatcaggacg gcgtgcgcct gaacgagtcg
     3961 ttcggcgaca acgtaaactg ggatctcgtt ccggaattcg ccgtcgaccg cctcaccgtc
     4021 cagagcaaca atccggtttt cgggctgaac gccatcggcg gcgcggtcac gctggcgatg
     4081 aagaatggat tggacttcga gggcggcgat gcggaactcg ggggcggttc gttcggaaat
     4141 ctcaacggca gcgccgaata tggcgcaagg atcggcaaac tcggaatcta tctgggaatc
     4201 ggaggtctcg aggacgatgg tttccgccat cattcgccca ccgcgctccg gcaggcctat
     4261 ggcgatcttg cctggcagaa cggcaggctg acgtttcatc tgtccgcatc gggagcgctc
     4321 gacaatatcg gcgcggtggg cccgacgccg gtgcaattgc tcgcgcagga tccgcgatcg
     4381 gtcttcacct ggccccaatc gaccgagaac ggaatggagc ttgtccagct gcgcggaaac
     4441 tggcgcgcca ccgatgagct ttcgctgagc gcgaacgcct atttccgccg cttcctccag
     4501 cacatcgtgg acggcaatac gaccgatgtc gtccgctgcg cgaacgattc ctccgagctc
     4561 tgcctcgagg gcaacgacct ctatccgggc gacgccctct acgatgcgca agggatgcaa
     4621 gtgccggcga gcgtgctgcc gcccggcgct acgccaggcg agaccgattt cactcgcacc
     4681 gacaccaaca gcgcgggggc tgcgatccag gcctcgttga cggctcccgt gctcgcccac
     4741 gccaacaatc tcacgatcgg ggcgtctttc gaccggggcg tcagcgatta tgctgcccat
     4801 ggcgagctcg gcacgctcac cgaaaatctc cacgtcctcg gttccggcgt gatcatcgat
     4861 caaggcctca gtccgaccgc tgagccgcca atcgaggaac ccgtgaacgt ggacgcgacc
     4921 aacctttatg cggggttcta cgcgatcgac gtcttcgatg tgacgccgca gctttcgtgg
     4981 acgctgagcg cgcggctcaa cctcgcgcag atcggattgc aggacaggct gggcgatgcg
     5041 ctcaccggcg accacgcctt cacccgtctc aatccgggca cgggcctcgc ctacaagcta
     5101 tccgacaggg tgacgctcta tggcggctat tccgaatcga accgcgcgcc gaccgcgggc
     5161 gagctgtcct gcgcga
//
LOCUS       DASZMC010000496         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1846346,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000496 DASZMC010000000
VERSION     DASZMC010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846346"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VG274_10210"
     CDS             <1..293
                     /locus_tag="VG274_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWE07072.1"
                     /translation="AQHLVKQLDHAAARAAVTAERAMLGTLGGGCQVPIGGYATVEGA
                     MIHLMAIVASPDGSRIIRSTLSGTDAEAVGATVGRDLLDKGARAILEEVYAA"
     gene            290..1768
                     /gene="cobA"
                     /locus_tag="VG274_10215"
     CDS             290..1768
                     /gene="cobA"
                     /locus_tag="VG274_10215"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR01469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWE07073.1"
                     /translation="MKVYLVGAGPGDPGLITVKGRRVLEHADSILYDHLASDRLLDLA
                     PAQCERVYVGKKRSAHEFSQEEISRMLIERARRGWNVVRLKGGDPFIFGRGGEEMEAL
                     AAAGIPFEIVPGVTTPLGLAAYTGVPLTHREHTSAVTFVTGHSVEAIDWSKVGACETI
                     VLFMGLVNFPSIARELIKHGRAAETPAMAVRWATRPDQQTIAGTLGDLAGRIEQAGMK
                     PPATIVIGEVVALRERFNWFEKLPLFGKRVVVTRDRRQSSMLAEPLAALGAEVLFVPV
                     IEIADAAESSPLEQAIRNLASYDWLIFTSVNGVRHFVEALDRSDQDLRSLRAKLCAIG
                     PATRAAVEALHLRVDVTPEEYVAESLLEALSGEDLKGKRILLPRAAVARDLVPVTLRE
                     RGAVVDVVEAYRTIIPADAAARAKEALAHKPDWITFTSSSTVKNLLAVTGTEAIAGIK
                     IASIGPVTSATAREAGLAVDVEADPHTIDGLVAAIARTPASR"
     gene            complement(1740..>2260)
                     /locus_tag="VG274_10220"
     CDS             complement(1740..>2260)
                     /locus_tag="VG274_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE07074.1"
                     /translation="HIGNGAGSATRTEDFITCQLAEPRLAASFIVDYHHLPDEFLRRA
                     LEAKGAERSILVTDAAAPALCAPGPYMLGGVAVELRDDDRVTLLGGNRLAGSSLRMDR
                     AIGNVMARAGVSLAHAVTMATTNPARVGRVPGRLRGLQPGSRADFVRFRMDGGRVEVL
                     ETWLSGKRVYAR"
BASE COUNT          404 a          650 c          797 g          409 t
ORIGIN      
        1 aagcgcagca ccttgtgaag cagctcgatc atgccgcagc gcgggcggcg gttacagcgg
       61 agcgcgcgat gctcggaacg ctcggcggag gctgccaggt tccgatcggc ggatatgcga
      121 cggtcgaagg agcgatgatt catctgatgg cgatcgttgc gtcgcctgat gggtcgcgaa
      181 tcattcgcag cacgctctcg ggcaccgatg ccgaagcggt cggagcaacg gtcggacgcg
      241 atctgctcga caaaggcgcg cgggcgatcc tcgaagaagt ctacgcggca tgaaggttta
      301 tctggtgggc gcgggtccgg gcgatccggg gctcatcacg gtcaagggcc gccgcgttct
      361 cgaacacgcc gactcgattc tctacgatca tctggcgagc gatcgtctgc tcgatctcgc
      421 gcctgcgcaa tgcgagcgcg tttatgtcgg taaaaagcgc tctgcgcacg aattttcgca
      481 ggaagaaatt tcgcggatgt tgatcgagcg cgcccgccgc ggctggaacg tcgtgcggct
      541 gaagggcggc gatccgttca tcttcggacg gggcggggaa gagatggaag cgctggcggc
      601 ggcgggcatc cccttcgaaa tcgtgccggg cgtgacgacc ccgctcggct tggccgccta
      661 caccggcgtg ccgctgacgc atcgcgaaca cacgtcggcg gtgacgttcg tcacggggca
      721 cagcgtggag gcgatcgact ggagtaaggt cggcgcatgc gagaccatcg ttctcttcat
      781 gggactcgtt aattttccgt cgattgcgcg cgaactgatc aaacatgggc gagcggcgga
      841 gactccggcg atggcggtgc gctgggcgac gcgtccggat caacagacca tcgcgggtac
      901 gctcggcgat ctggcagggc ggatcgagca ggcaggcatg aagcctcccg cgacgattgt
      961 aattggcgaa gtggtggcgc tgcgcgaacg tttcaattgg ttcgagaagc tgccgctgtt
     1021 cggaaagcgc gttgtggtta cccgcgaccg cagacaatcg tcgatgctgg ccgagcctct
     1081 cgcagcgttg ggcgcggagg tcttgttcgt accggtgatc gagatcgcgg acgcggcgga
     1141 gtcgagtcct ttggagcagg cgattcggaa tctcgcttcc tacgactggc tgatcttcac
     1201 gagtgtcaac ggcgtgcggc attttgttga agccctggac cgctcggatc aagatttacg
     1261 gtctttgcga gcgaagctgt gcgcgattgg accggccacg cgggcggcgg tggaggctct
     1321 gcatctgcgc gtggatgtga cgccagagga atacgttgcg gagagtttgc tggaggcgct
     1381 gtcgggtgaa gacctgaagg gcaaacgcat tctgttgccg cgtgcggcgg tggctcgcga
     1441 tttagttcct gtgacgctgc gtgaacgcgg cgcggtcgtg gatgtggtcg aagcctatcg
     1501 caccatcatt ccggcggatg ccgccgcgcg ggcgaaagaa gcgctggcgc ataaaccgga
     1561 ctggatcacg ttcacaagtt cgtcgacagt taagaacctg ctggcggtga cgggcacgga
     1621 ggcgatcgca ggaataaaga tagcctcgat tggtccggta acctctgcaa ccgcgcgtga
     1681 ggccgggctg gctgtcgatg tcgaagcaga cccgcacaca atcgacggac tggtcgccgc
     1741 tatcgcgcgt acacccgctt cccgctgagc cacgtttcaa gaacctcaac ccgcccgccg
     1801 tccatgcgga agcgaacaaa atccgcgcgc gatccgggct gaagaccgcg caggcgcccg
     1861 gggactcggc cgacgcgcgc ggggttggtg gtcgccatgg tgacggcgtg cgcgagactt
     1921 actccggcgc gtgccatcac gttgccaatg gcgcgatcca tgcgcaagct ggatccggcc
     1981 agtcgattcc cgccgagcag cgtgacgcgg tcgtcgtcgc gcaattcgac ggcgacgcca
     2041 ccgagcatat acggtcccgg agcacagagc gcgggcgcgg cagcgtcggt caccaggatg
     2101 ctgcgttcgg cgcctttggc ttcgagagcc cggcgcaaga actcgtcggg cagatgatgg
     2161 taatcgacga tgaaggacgc tgccaggcgc ggttcggcaa gttggcaagt gatgaagtct
     2221 tcggtgcggg tggcactccc ggccccattg ccgatgtggg
//
LOCUS       DASZMC010000497         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1846386,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000497 DASZMC010000000
VERSION     DASZMC010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846386"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VG274_10225"
     CDS             complement(<1..294)
                     /locus_tag="VG274_10225"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWE07075.1"
                     /translation="MSQTAPAFAARDPEEVEGLGAAASGANLFVLKQGDTFLVADVFG
                     DVRGEADGLFHNDTRVLSRLRLLLGGGRPSLLGGTVSQDNVFFIAHLTNHPLAP"
     gene            complement(300..1364)
                     /locus_tag="VG274_10230"
     CDS             complement(300..1364)
                     /locus_tag="VG274_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWE07076.1"
                     /translation="MRIAQIAPLTEAVPPLLYGGTERVVAHLCNALAEMGHEVTLFAS
                     AEARTRARLVPMRAQAIRLDASPLKSDVAAHLVMLDEVRRRKDDFDILHFHLDLLHFP
                     FFEDIAERTLTTLHGRLDLKDLDGVYARWSAYPLVAISNRQRAPLPDANWIATVPHGL
                     PIDQFKFTPSPEGGYLAFLGRVSPEKGVDRAIALARAADVPLKIAAKIDPFDVPYFNG
                     RIEPLLHDPMVEFVGEIADADKPAFLGNALALLFPVDWPEPFGLVLIEAMACGTPVVA
                     WDRGAVPEIVEDGVSGFVVRSHEEALQAIGKLKSVSRLRIRRVFERRFSASAMARRYL
                     EVYARLLRGAACDGTFKDAV"
     gene            1553..1837
                     /locus_tag="VG274_10235"
     CDS             1553..1837
                     /locus_tag="VG274_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HWE07077.1"
                     /translation="MRELLVMFVTIFVAELGDKTQIATLLFASEHKAPPLGVFCASAG
                     ALVIGAALSVLIGTAASRYLEFVPLKLIAGLGFVAIGIWTLIEYFRGAAA"
     gene            1908..2738
                     /locus_tag="VG274_10240"
     CDS             1908..2738
                     /locus_tag="VG274_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE07078.1"
                     /translation="MPIGADTMSCLEWRAPDAATMLFVHATGFNAETYRALLAPLALQ
                     FHVFACDLRGHGFSTLPTRPGLAEGWTIFRDDIVMLLGQLTAGPVIVAGHSLGATVGL
                     MAAAADPSRVRALILVEPVLAPSAGMDDRKPSTGLALRAAQRRSIFPSPKAALDSYRG
                     RGIFASWPDSAIGDYLAGGLIEAGDDTWRLACAPAWEAEIFHEAPGGIAQAARDIACP
                     MSILHGSIASTSTASELALIRRLKPDARVVTLEGGTHFLPIEQPGRVAAQMLSALKGL
                     "
     gene            complement(2753..3319)
                     /locus_tag="VG274_10245"
     CDS             complement(2753..3319)
                     /locus_tag="VG274_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWE07079.1"
                     /translation="MRARNATRSAILDAARRVAARDGARNLSLRAAAAEAGFAPAALY
                     GYFQSKDEILLALAADDLLAIARAMRAAAENSGAESPLAAASSAALALLQHTETIAAA
                     SQALPQTAGTGEAERLFNGRLIAALTALSHATGRTAESRTAQADIVLLAAALTGLAVF
                     ARSGRLGALGFSTEEVVARLDARFTEPM"
     gene            complement(3384..3827)
                     /locus_tag="VG274_10250"
     CDS             complement(3384..3827)
                     /locus_tag="VG274_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07080.1"
                     /translation="MLTRSGFAFLVLLASGGAAAAQSMCGEPPIAPVIPTAAEIRQKP
                     AADAATVRHNAFLDIKRWQASLKSYRDCLSATINTDKRDLYEDARASKPDKDKMASIQ
                     QEMKASNDASDASANEEEKVVNDFHAAQVAYCTRTDVDVSTCPKN"
BASE COUNT          710 a         1303 c         1261 g          716 t
ORIGIN      
        1 cggagcaagc ggatggttcg tcaaatgcgc gatgaagaac acattatcct ggctgacggt
       61 tccgccgaga agcgatggcc ggccaccgcc gagaagcagc cgcaaacggg agagcacgcg
      121 cgtgtcgttg tggaacaggc cgtccgcctc tccacgtaca tcgccgaaga cgtcggcgac
      181 cagaaacgtg tcgccctgct tgaggacgaa cagattggcg cccgacgcgg cggcgccgag
      241 tccctccact tcttccggat cgcgggcggc aaaagccggc gctgtctgcg acatgtggtt
      301 cagactgcgt ctttgaacgt gccatcgcaa gccgcgcccc gcagcaggcg cgcatagact
      361 tcgaggtagc gcctcgccat cgcgcttgcc gagaaacggc gttcgaagac acgacgaatg
      421 cgcaggcggc tgacgctctt caacttcccg atcgcctgca gcgcctcttc atgcgagcgg
      481 accacaaaac ccgacacgcc atcttctacg atctcgggca cggcgccgcg atcccaggcc
      541 acgacgggcg tgccgcacgc catcgcctcg atcaacacca ggccgaacgg ctccggccaa
      601 tcgacgggaa acaggagcgc cagcgcgttg ccgaggaaag ccggcttgtc cgcatctgcg
      661 atctcaccca cgaattccac catcggatca tgtagcagcg gctcgatccg gccgttgaaa
      721 taggggacgt cgaatgggtc gatcttggct gcgatcttga gcggtacgtc cgccgctcgc
      781 gcgagcgcaa tggcgcgatc cacgcctttc tccggcgaaa cgcggccgag aaatgcgaga
      841 tacccgccct cgggcgaagg agtaaatttg aactggtcga tgggcaagcc gtgcggcacc
      901 gtcgcgatcc agtttgcgtc cggcagtggc gcgcgctgac ggttggagat tgcgaccagc
      961 ggataggccg accagcgtgc atagactccg tcgagatcct tgaggtcgag gcgtccgtgc
     1021 agcgttgtaa gcgtccgctc ggcgatgtcc tcgaaaaagg gaaaatgcaa aagatcgagg
     1081 tgaaaatgca ggatatcgaa atcgtctttg cgccggcgca cctcgtcgag catgaccaga
     1141 tgggcggcta cgtccgattt caggggactg gcatccaggc gtattgcctg ggcgcgcatg
     1201 ggaacgagcc tggcgcgcgt tcgcgcctcg gcgctggcaa acagggtgac ctcgtgaccc
     1261 atttccgcca gtgcgttgca gagatgggcc accacccgtt cggtcccacc gtacaaaagc
     1321 ggcgggacgg cttcggtgag cggagcgatc tgtgcaattc tcattttgat ccttcttggt
     1381 tcctggacgc aggccgacct accctgcccg ccggtttctg cccgcattta aaacggcacg
     1441 gccgcccttt tggtgcccgt cagaatcgtg gaaattcccc cgggctcgcg gacgcggcag
     1501 tgcgggcgca tatcggacga tgtttcatcg aaccactacg gacggagacc ccatgcgcga
     1561 attgctcgta atgttcgtca ccatcttcgt cgcggagctg ggcgacaaaa cgcagatcgc
     1621 gaccctgctc ttcgcttccg agcacaaggc gccgccgctg ggcgtcttct gcgcttcggc
     1681 cggcgctctc gtgatcggcg cggctctttc ggtgctgatc ggcacagccg caagccggta
     1741 tctcgagttc gtgcccctca agctgatcgc cggactgggc ttcgtcgcca tcggaatctg
     1801 gaccctgatc gaatattttc gcggcgccgc cgcttgaccg gcccatcgct tccactgctc
     1861 gacacatcag cctgacggtg aggagcaacg atcccgtccg gacggctgtg cccatcgggg
     1921 cggacacgat gtcgtgcctc gagtggcgcg cgccggatgc cgcgacgatg ctttttgtcc
     1981 atgcaaccgg gttcaacgca gagacctatc gcgctctgct tgcgccgctc gctttgcaat
     2041 ttcacgtgtt cgcctgcgac ctgcggggtc acggattctc cacgctgccg acgcgacctg
     2101 gtcttgccga gggatggacg attttccgcg acgatatcgt catgctgctc gggcagctga
     2161 ccgccgggcc tgtgattgtc gccggtcatt cgctgggagc gacggtcggc ctcatggcag
     2221 ccgcggctga tccttcccgc gtccgtgcct tgatccttgt cgagccggtt ctcgcgccgt
     2281 cggcgggcat ggacgatcgc aagccgtcga ccggtctcgc actcagagcc gcccagcgcc
     2341 gcagcatatt tccgtctccc aaagccgccc tcgattccta tcgcggccgc ggaatattcg
     2401 cctcctggcc ggattccgcg atcggggatt atctcgcggg cgggctgatc gaggccgggg
     2461 acgacacatg gcgactggcc tgcgcgcccg cgtgggaggc agaaattttc cacgaagccc
     2521 caggcggcat tgcgcaggcc gcgcgcgaca tcgcctgtcc catgagcatt ctgcacggct
     2581 cgatcgcgag cacgtcaacc gcgtccgaac tcgcgctgat ccggcgcctt aagcctgacg
     2641 cgcgggtggt gacgcttgaa ggtgggacgc acttcctgcc gatcgaacag cccgggcgcg
     2701 ttgcggcgca aatgttgagt gccttgaagg gattgtagaa tcgcagccac ccttacatgg
     2761 gttcggtgaa gcgcgcatcg agacgcgcaa caacttcctc ggtggaaaat cccagcgctc
     2821 cgagccggcc cgagcgggcg aagacggcga gccccgtcaa cgcggcagcc aacaaaacaa
     2881 tatccgcctg cgccgtgcgg ctctccgccg tgcggcccgt tgcatgcgaa agtgcggtaa
     2941 gcgccgcgat caagcgcccg ttgaacagac gttccgcctc gcccgtgccg gccgtttgcg
     3001 gcaaggcctg cgaggccgcg gcaattgttt ccgtatgctg cagaagcgcc agtgcggcgg
     3061 aggatgctgc ggcgaggggg ctctccgcgc cgctattctc cgctgcggct cgcatcgcgc
     3121 gcgcgatcgc caggagatcg tccgcagcga gggcaagcaa aatctcgtcc ttgctctgaa
     3181 aatagccata gagagcggcg ggggcgaaac ccgcctcggc cgcagcggcg cgcagcgaaa
     3241 gattgcgggc gccgtctcgc gccgccaccc gccgcgccgc atcgagaatc gccgagcggg
     3301 tcgcattgcg cgctctcaag cggcgttgga tacgcgggtc gcctgccatg cccgcgaaca
     3361 taagcgattc gcggtgggac ttgtcagttc ttggggcagg tggaaacgtc gacatcggtg
     3421 cgcgtgcagt aggcgacctg cgccgcgtgg aagtcgttca ccaccttttc ctcttcattg
     3481 gctgaagcat ccgacgcatc gttcgacgcc ttcatctcct gctggatcga agccattttg
     3541 tccttgtcgg gctttgaagc gcgcgcgtcc tcgtagagat cgcgcttatc ggtgttgatc
     3601 gtcgcgctca ggcagtcgcg ataggatttg agcgaagcct gccaacgctt gatgtccagg
     3661 aaggcgttgt gccggaccgt cgccgcatcc gcggcgggct tctgtctgat ttcggcggca
     3721 gtcggaatga cgggcgcaat cggcggctcg ccgcacatgc tctgggctgc agccgctccc
     3781 cccgaagcta gcagcacaag gaatgcgaaa cccgatctcg tcagcatgcg aacccctggc
     3841 cgtgtcttcg acgagatcga taagagcaca ccacacgctc ccggataaag ccccgaaact
     3901 caaacccccg cggcgtaggg aatgccgatc ctgctcatgt ggagtccccg cgacgcgcaa
     3961 acgcaccggc cagaccctcg acgggagagc
//
LOCUS       DASZMC010000498         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5147495,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000498 DASZMC010000000
VERSION     DASZMC010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5147495"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..1163)
                     /locus_tag="VG274_10255"
     CDS             complement(423..1163)
                     /locus_tag="VG274_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWE07081.1"
                     /translation="MRIAIVSDTHDSLIALEAVLADLRDASPDLVLHGGDLVGGGSSP
                     AEVVDRIRNLGWPGVFGNTDEAIARPETLEEFASQSSAPPLLWDKVREMTAFTRAALG
                     KDRVAWLAALPRVIIHPPVALVHASPASAWRSPGAEATNAELESAYQALGQPIAVYGH
                     IHQPFIRRLAGITVINTGSISQSFDGDPRASYLLIDDGAPEIRRVEYDVEREIRAVAA
                     SGLPHADWVARTLRRPPADALITREETA"
     gene            complement(1172..>1778)
                     /locus_tag="VG274_10260"
     CDS             complement(1172..>1778)
                     /locus_tag="VG274_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWE07082.1"
                     /translation="TIDQRYSVGSVVEGRVRNMTEFGAFIEIEEGIDGLVHISDLSWT
                     KRVKHPSEILRKGSVVQAAILAIDSKNKRLSLGIKQLQPDAWETFFQSHNVNDTARGK
                     VLRLAGFGAFVELAEGVEGLCHFSEVPGWSGKRADPPPLNPGDEVDFKIIRMNETERK
                     IGLSIKALADVEERNRLDEYRKRAAAASSSFEDYAPRSDRR"
BASE COUNT          323 a          544 c          581 g          330 t
ORIGIN      
        1 ggccgccgca aggatgtttt cgcgaacgat ctgcggtgat gggttgccgc gcggcataca
       61 aagtatccta ctgcaatgta ataccgcgcg agaaagcgcc gaatggccag aggcgccctg
      121 aacgtgagcg agatcaggca gcccggatgc agcccgcgcc cggcggtcaa ctccttgacc
      181 gggcaaacgg acggaaacgt aatcagcggt cgcgacggat agatctatcg gctcggcgcc
      241 cgcacggttg gcggcgtcga cactgccact ctcgcgagcg agatgcagcc aaatggcggc
      301 gagctgctct tccgtctcag gcgtcttcca cctcaggcga aagacgaaac tcgctcatct
      361 gcgcttgttc tactcgatgg ccaacggcgc catgccgcgc cacctgtgat gtcgtctgcg
      421 agtcaggcgg tttcctcgcg cgtgatcagg gcatctgcgg gcgggcggcg gagcgtccgc
      481 gcaacccagt cggcatgcgg cagcccgctc gcggcgacgg cacggatttc ccgttcgacg
      541 tcgtattcaa cccggcggat ctctggcgcg ccgtcgtcga tcagcagata ggaagcgcgg
      601 ggatcgccgt cgaaagactg gctaatgctg cctgtgttga ttacggtgat cccggccagg
      661 cggcggatga acggctggtg gatgtgaccg tagaccgcga tgggctgccc gagggcctgg
      721 taagccgatt cgagttccgc attggtggct tcggcgccgg gcgagcgcca cgcgcttgcg
      781 ggactggcgt gcaccagcgc gacgggaggg tgaatgatga cgcgcggaag agcggcgagc
      841 caggcgactc ggtctttgcc caaagctgcg cgagtgaagg cggtcatctc gcgcacctta
      901 tcccacagca atggcggcgc ggaagactgg cttgcgaatt cctccagcgt ttccggcctc
      961 gcaatggctt cgtccgtgtt gccgaaaacg ccgggccagc ccagattgcg aatacggtcc
     1021 accacctccg cgggactgga tccgccgccc acaaggtcgc ctccatggag gaccagatcc
     1081 ggcgaagcgt cgcgcagatc ggcgagaacg gcttcgagcg cgattagact atcgtgggta
     1141 tcggatacga tggcgatccg cacgccgagc gctaacgccg gtcggaacgg ggagcgtagt
     1201 cctcgaagct cgagcttgcc gcggccgcgc gcttgcggta ttcatcgaga cggttacgct
     1261 cctcgacatc ggccagggct ttgatgctga ggccgatttt ccgctccgtc tcattcatgc
     1321 gaatgatttt gaagtccact tcatcgcctg gattcagggg cggcggatcg gcgcgtttgc
     1381 cgctccagcc gggcacttcg gagaagtggc aaagcccttc gacgccttcg gccagttcca
     1441 cgaaggcgcc aaaaccggcg aggcgaagca cttttccccg cgccgtatcg ttcacattat
     1501 ggctctggaa gaacgtttcc caggcgtccg gctgcaactg cttgatgccg agcgacaggc
     1561 gtttgttttt ggaatcgatg gcgagaatcg cggcctgcac gacggagccc ttgcgcagga
     1621 tttcgctcgg atgtttgacg cgtttggtcc aggaaagatc cgagatgtgc acaaggccgt
     1681 cgatgccttc ctcgatttcg atgaaagcgc cgaactcggt catgttgcgg acgcggccct
     1741 ccactaccga cccgacgcta tagcgctgat cgatcgtg
//
LOCUS       DASZMC010000499         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1847663,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000499 DASZMC010000000
VERSION     DASZMC010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847663"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="cysE"
                     /locus_tag="VG274_10265"
     CDS             <1..753
                     /gene="cysE"
                     /locus_tag="VG274_10265"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HWE07083.1"
                     /translation="ASELAESEPSLASLAHATILNHEQLEDALSYHLARKIGSEELSA
                     MQTREVFDEAMGEDEEIGAAVRADLSAVFERDPACHSYVQAFLFFKGFHALQCHRIAH
                     WLWEKDRTPLAHLLQSRVSELFAVDIHPAAKIGRGIMMDHATGIVIGETAVVEDDVSM
                     LHGVTLGGSGKEKGDRHPKVRRGVMLSAGAKVLGNIEIGEYSRVGAGSVVLKPVPAHC
                     TVAGVPAQVIGCAGSDCPSQTMDQLIEALESD"
     gene            complement(815..1408)
                     /locus_tag="VG274_10270"
     CDS             complement(815..1408)
                     /locus_tag="VG274_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWE07084.1"
                     /translation="MPGAAHFITKVFAFALMLTVAGCGRPARSDTTSIAGVMPRLAFA
                     MIRANDDAAVTAANYRGKAVLLYFGYTHCPDECPTTLANLASALKLLGPNAERVRVLF
                     VTVDPSRDTIPALKSYVKVFAPQIDGLRGSANAVEELARRYRVIYEVTPVSPAHSYSV
                     MHSDSVFMFDGSGRAQFVTLQTNDTAALARRIAQLGS"
     gene            1521..>2436
                     /locus_tag="VG274_10275"
     CDS             1521..>2436
                     /locus_tag="VG274_10275"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWE07085.1"
                     /translation="MSILSTTRTEARPATVDRNRREWLYYCALLVVGASISVACRFFP
                     ADLPFWMPWDFSWPVFLLTGLGLGWFALGLARLPQSLRPAIWRQVSFVLGTLSIYAVL
                     QTHVDYFAQHMFFVHRGQHFVLHHLGAFLIALGMPGQAIWVGMPRFLRPLLASRPVRA
                     VVDFIQHPAVAPVVFVGLIYLWLIPAFHTRVMLDADLYNIMNASMAVDGIFFWCLVLD
                     NRPHPPARIGSGLRALLLIAVEPFQMVLGAVLSLAHTDYYPVYRICGRIWDIPALSDQ
                     HYGGLIIWLPSTLTSFAGVICVLVAMRIN"
BASE COUNT          426 a          720 c          764 g          526 t
ORIGIN      
        1 gcttccgagc tggccgagag cgaaccctcc ctcgcgagcc tcgcgcacgc cacgatcctc
       61 aatcacgaac aactcgagga cgcgctctcc tatcatcttg ccaggaagat cggcagcgag
      121 gaactgagcg cgatgcagac gcgcgaggtg ttcgacgagg ccatgggcga agacgaagaa
      181 attggcgcag cggtcagggc agacctttcc gccgttttcg agcgcgatcc ggcctgccat
      241 tcctatgtcc aggcgtttct tttcttcaag ggatttcatg cattgcagtg ccatcgcatt
      301 gcgcattggt tgtgggagaa ggatcgcacg ccactcgccc atcttctgca gagccgtgtt
      361 tcggaacttt tcgcggtcga tatccatccg gcggcaaaga tcgggcgcgg catcatgatg
      421 gaccacgcga ccggaatcgt gatcggtgaa accgctgtgg tcgaagacga tgtctcgatg
      481 cttcatggcg tgacgctggg cggatcgggg aaagagaaag gcgaccgtca tccgaaggtt
      541 cgccgtggcg tcatgctgtc ggctggcgcc aaggtgctcg gcaatatcga gatcggcgag
      601 tattcgcgcg tgggcgcggg gagcgtggtt ctcaagcctg tgccggccca ttgcacggtg
      661 gcaggggttc ctgcgcaagt catcggctgc gccggttcgg actgcccgtc ccagaccatg
      721 gaccagctga tcgaagcgct tgaatcggat tagcgaaagc ggttttcggg tgacaagcgc
      781 ccgttgccat acctcgcatt cgcgctattc cgccctacga cccgagttga gcgatgcgcc
      841 ttgcaagcgc agcggtgtca ttggtttgaa gggttacaaa ttgggctcgt ccggacccgt
      901 cgaacatgaa caccgagtcg gaatgcatga cggaatagga atgagccgga gagacaggcg
      961 tcacttcgta gatcacgcga taacgcctcg cgagttcctc cacggcgttt gcgctgcccc
     1021 gcaaaccatc aatctggggc gcgaacactt tcacgtagga tttgagagcc ggtatcgtgt
     1081 cgcgcgacgg atccaccgtg acgaacagca cgcgcacacg ctccgcgttg ggtccaagca
     1141 gctttagcgc cgaggcgagg ttggccagtg tcgtcggaca ttcgtcggga caatgcgtgt
     1201 agccgaaata gagcagcacc gccttgccac gatagttcgc ggcggtaacg gccgcatcgt
     1261 cgttcgcgcg gatcatcgcg aatgcgaggc gcggcatcac gccggcgata ctggtggtgt
     1321 cgctgcgcgc cggcctgccg caaccggcca cagtcaacat cagcgcaaac gcgaacacct
     1381 tcgtgatgaa atgggcggca ccgggcatcg tcgcgggaga tagcatggcg cggcgcgata
     1441 aggctaaaac ccgcagcgca aatctcctgt gaccaatcgc cccggcgaac gggcaggagg
     1501 cgagactata agggccggcg atgtccattc tttcgacgac gcggacagag gcgcgccccg
     1561 cgactgtcga tcggaacagg cgcgaatggc tgtattattg cgctctcctt gttgtgggcg
     1621 cgtccatttc ggtcgcctgc cggttttttc ccgccgatct gccgttctgg atgccttggg
     1681 atttctcctg gccggtcttt ctcctcacgg gtctcgggct cggctggttt gcgcttggcc
     1741 tggcgcgcct gccacagtca cttcgccccg cgatatggag gcaggtctcg ttcgtgctgg
     1801 gcacactttc gatctacgcc gtgctgcaga cccatgtcga ttatttcgcg cagcacatgt
     1861 tcttcgtcca tcgcggtcag cattttgtgc tgcatcatct gggcgcgttt ttgatcgcgc
     1921 tgggcatgcc gggtcaggcg atctgggtcg ggatgccgcg ttttttgcgt ccgctccttg
     1981 cttcgaggcc tgtgcgtgcc gttgtcgatt tcatccagca tcccgcggtg gcgcccgttg
     2041 tgttcgtcgg gctgatctat ctttggctga ttccggcctt tcacacccgc gtgatgctcg
     2101 atgccgatct ctacaacatc atgaacgcga gcatggcggt ggatggcatc ttcttctggt
     2161 gcctcgttct ggataaccgg ccgcatccac cggcgcgaat cggaagcgga ctgcgcgcgc
     2221 ttctgctgat tgcagtcgaa ccgtttcaga tggtgctggg tgcggtgttg tcgcttgcgc
     2281 acacagacta ttatcccgtc taccgtattt gcggccgcat ctgggatatt ccggcactaa
     2341 gcgaccagca ctatggaggg ttgatcatct ggctgccgag cacgctcacg agcttcgcgg
     2401 gcgtgatctg cgtgctcgtg gcgatgcgca tcaacg
//
LOCUS       DASZMC010000500         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2682825,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000500 DASZMC010000000
VERSION     DASZMC010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2682825"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VG274_10280"
     CDS             <1..296
                     /locus_tag="VG274_10280"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWE07086.1"
                     /translation="NGLARRKNVVAEASSGIEAQLKRRADLIPNLVETVKGSALLNMF
                     RRRDGSLAFASGKVLSRMDSAEAIADLASHCLRLLTAVSTLVFNYQPDDASLQ"
     gene            500..781
                     /locus_tag="VG274_10285"
     CDS             500..781
                     /locus_tag="VG274_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07087.1"
                     /translation="MTPHVHVRGAKKPRVRATLEQARKTGLLGGVKDTRISGRVSRRL
                     VTAAKRSAGAQSDTDVIEIALATLALEDDFGQKLVRRKGSVPRDLDLGL"
     gene            771..1340
                     /locus_tag="VG274_10290"
     CDS             771..1340
                     /locus_tag="VG274_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07088.1"
                     /translation="MGFDLDAALRWLKPQRRAKPLIRRADDELPWIGDAPPIGPPVLL
                     DSTVYIDTLQGRSPVALDALVSLRTCNHSAVCLSELSHAFGRLNPTDARTPGALKALR
                     QTTRDIPAHRLSAPDVELWGIAGILAGILFRLGSFARGAERKCLNDALVYLQARKLGI
                     SVLTGNIRDFDMLNQLVPDGRVMMYRRQP"
     gene            1411..1995
                     /locus_tag="VG274_10295"
     CDS             1411..1995
                     /locus_tag="VG274_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07089.1"
                     /translation="MRTLFVTLGAAAACLLLAGCDQNSSSKSNDVTVSGHDGNVTISA
                     NGQKFTMKAGDGKSGNFTMSGDNGHFTMHASDGKQTVDINATGDTSTLKLPGFVAVYP
                     GAKVQSTVIGAGAGANDGGGTFTFETTDSPAAVIAFYKEKSASQGLAQAINMSTGTTT
                     MFSANSDKTKRAIQVVAASSGSGARVQVNWSGAD"
     gene            1996..2274
                     /locus_tag="VG274_10300"
     CDS             1996..2274
                     /locus_tag="VG274_10300"
                     /inference="COORDINATES: protein motif:HMM:NF023158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HWE07090.1"
                     /translation="MAIAVTSDSIPDAPPDRLSVNPKSPHYDDAVLKRGVGVRFNGVE
                     KHNVEEYCVSEGWVRLAAGRSVDRAGNPMTVKLKGTVEPYFEDIPREA"
     gene            complement(2225..2743)
                     /locus_tag="VG274_10305"
     CDS             complement(2225..2743)
                     /locus_tag="VG274_10305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE07091.1"
                     /translation="MDMLRTPGPADLDALLALSNVHEREIGAIAKPAFEELVFMSFRT
                     RMTEAGDAFLIALADRAPEVAPNYHWFAGRFDRFVYIDRVVVAAASRKRGLATLLYRD
                     LIDASRRAGHTRLCCEVNSDPPNPASDAFHATFGFEEIGRAYLPDRGKTVRYLTLTLR
                     GECPRSTARLCP"
     gene            3091..3657
                     /gene="efp"
                     /locus_tag="VG274_10310"
     CDS             3091..3657
                     /gene="efp"
                     /locus_tag="VG274_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWE07092.1"
                     /translation="MPKIAGNEIRPGLLIAHEGGLWLVVKSQAVKAGRGGAYNQVELK
                     NIVTGTKLNQRFRADETVEETSLEKRDFQFLYASGDALTFMDLESFDQIDVPRDFVGE
                     QLPFLQDGMKVLLQLHEGRPISIALPQQVILSVAQADPVLRGQTAAPGYKSAVLENGL
                     RVLVPAFIEPGARVVVATTGGTYVRRAE"
     gene            3658..>3951
                     /locus_tag="VG274_10315"
     CDS             3658..>3951
                     /locus_tag="VG274_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWE07093.1"
                     /translation="MPYLSPALNVMTTAARKAGRGLIRDFGELENLQVSLKGPADFVT
                     AADRRTERILIQELSRARPGYQFLVEEAGAIEGPDRSHRFIIDPLDGTTNFLHG"
BASE COUNT          753 a         1202 c         1268 g          728 t
ORIGIN      
        1 tcaacggcct cgcgcggcgc aagaacgtcg tggccgaagc gtcgagcggt atcgaagcgc
       61 agctcaagcg ccgcgccgat ctcattccca atctggtcga gacggtgaag ggcagcgcct
      121 tgctcaacat gttcaggcgc cgggatggtt cgttggcatt tgcatccggt aaggtgctat
      181 cgcgaatgga ttcggcagag gcaatcgcgg atctcgcaag tcattgtctt cggctcctca
      241 ctgcggtctc caccctcgta tttaactatc agccggatga cgcctcgcta caataagtga
      301 cttccgcgac aggaaggcaa ttgagctcca gaccggcgcg cctcaggcgg tgcattgggc
      361 caagcgcctg agagctttca ccccggattt ttcgttgctc ggcggaatgc gcaggccaaa
      421 taggtttcct agctcagttc gcgtggactc cgtgttcaac atatgccata cttcgttgaa
      481 cggttcagag aggttggaaa tgacgcctca tgttcacgtt cgcggcgcaa aaaagccgcg
      541 cgtcagggcc acgctggagc aggcgcgcaa aacgggactg ctcggcggcg tcaaggacac
      601 gcgcatttcc ggacgcgtct cccggcggct ggtcacggcg gccaagcgga gcgcaggcgc
      661 gcaatccgat acggacgtaa tcgagatcgc cctggcgacg ctcgcgctcg aggacgattt
      721 cggccagaag ctcgtccgcc gcaagggctc ggtcccgcga gacctcgatc ttgggctttg
      781 atctcgacgc tgcgcttcga tggctgaagc cgcagagacg tgcgaagccc ctgatccggc
      841 gagcggacga cgagctcccc tggatcggcg atgcaccacc gatcggtccg cccgtgctcc
      901 tggattcgac cgtctacatc gacacattgc agggccgttc gccggtcgct ctggatgcgc
      961 tcgtcagcct gaggacgtgc aatcattcgg cggtttgcct ttcggaactc tcgcacgcat
     1021 tcgggcggtt gaacccgact gatgcacgaa ctccaggcgc tctcaaggcg ctgcggcaaa
     1081 caacccgcga tattcctgcc catcgtctct ccgcgcccga tgtcgaactt tggggcattg
     1141 ccgggattct ggcgggcatc ctgttccgcc tcggcagctt cgcgcgcggc gccgagcgca
     1201 aatgcctgaa cgatgcgctc gtctatctcc aggcccgcaa gcttggtatt tctgtcctta
     1261 ccggaaacat tcgggatttc gatatgttga accagcttgt gccggacggg cgcgtgatga
     1321 tgtaccggcg tcagccgtag tctgaggctt gtgctgccga gcgtttccgg cgaatggttg
     1381 gcccggggcg tcgatgcaaa gggaaattcc atgcgcactt tatttgtgac gcttggcgcg
     1441 gccgcggcct gcctgctgct cgcgggctgc gaccagaatt cgtcgagcaa gtccaacgac
     1501 gtcacagttt ccggtcatga cggcaatgtc accatctccg ccaacggcca gaaattcacg
     1561 atgaaggcgg gcgatggcaa gagcggcaat ttcaccatgt cgggcgacaa cgggcatttc
     1621 accatgcacg cctccgacgg caagcagacc gtggacatca acgcgacggg cgatacgtcg
     1681 accttgaagc tgccgggctt cgtcgccgtc tatccgggcg cgaaggtgca atcgaccgtg
     1741 atcggtgcgg gtgcgggtgc gaatgacgga ggcggcacat ttacgttcga aaccaccgat
     1801 tcgcctgctg ccgtgattgc tttctacaag gagaagtcgg caagccaggg cctcgcccag
     1861 gcgataaaca tgtccaccgg cacgacaacg atgttctccg ccaattcaga caagacaaag
     1921 agggcgatcc aggtcgtcgc cgcctcgtcc ggcagcggcg cgcgcgtcca ggtgaactgg
     1981 tcgggcgcag actgattggc cattgccgtg acgagcgatt ccatacccga cgcgcctccg
     2041 gatcgcctga gcgtcaatcc gaagagcccg cattatgacg atgcggttct caagcggggc
     2101 gtgggggtgc gcttcaatgg cgtggagaag cacaatgtcg aagaatattg cgtgagcgaa
     2161 ggttgggttc gcctggccgc agggcgaagt gtcgaccgcg cgggcaaccc gatgacggtc
     2221 aagctcaagg gcacagtcga gccgtacttc gaggacattc cccgcgaagc gtgagcgtca
     2281 gatagcgcac cgttttgccg cgatcgggta ggtaggcgcg gccgatttct tcgaagccga
     2341 acgtggcgtg aaaagcatcc gatgccggat tgggtggatc gctgttcact tcgcagcaga
     2401 gtcgcgtatg acccgcgcgt cgggaggcgt cgatgagatc gcgatagagc agcgtggcca
     2461 gaccgcgctt gcgcgaggct gcggccacca ccacgcgatc gatgtagacg aatcggtcga
     2521 agcggccggc gaaccaatga taattgggcg cgacttcggg cgcgcggtcg gcaagcgcga
     2581 tgaggaacgc gtcgcccgcc tcggtcatcc gtgtgcggaa cgacatgaag acaagctctt
     2641 cgaacgcggg cttcgcaatc gcgccgattt cgcgctcatg cacattgctg agagccagca
     2701 gcgcgtcgag atcggcagga ccaggtgtgc ggagcatgtc catgtccgtt cgcagctcgt
     2761 gccgtttgcg cccgcgcggc ccctgctgct agaacgcgcg acattccgcc agagacctcc
     2821 ataatggaat ctcatgagtc tgcaatcctg ggaccgcggg catcccgccc gcaccggggg
     2881 cggcaccaag ggcgcggggt aaacggcgcg gtttgggcgg cagattattt cgatcgcttc
     2941 attcgcgacg gcatacattt tgccagtgca gtctcgtaca ttgaagacaa tccggtgaag
     3001 gcgggaccgt gccggcactc ccgcgactgg ccgtactcct cggcaagcga aagtgcgggc
     3061 gggacgcccg cggtcccggg ataatttgtg atgcccaaga ttgccggcaa cgaaatccgc
     3121 cccgggcttc tcattgcgca tgagggcgga ctatggctcg tggtgaagag tcaggcggtg
     3181 aaagccggaa ggggcggcgc ctacaaccag gtcgagctga agaacatcgt caccggaacc
     3241 aagctcaacc agcgctttcg cgccgacgag accgtcgagg aaacctcgct cgagaagagg
     3301 gacttccagt ttctctacgc cagcggcgac gcgctgacct tcatggattt ggagagcttc
     3361 gaccagatcg atgtgccgcg cgattttgtg ggcgagcaat tgccgttcct gcaggacgga
     3421 atgaaggttc tcctccagtt gcatgaaggc aggccgatct ccatagcgct tccacagcag
     3481 gtgatactct ccgtcgcgca ggcggatccg gtgctgcgcg gccagaccgc tgcacccggc
     3541 tacaaatcgg cggtgctcga aaacgggctg cgcgtgctcg tgcccgcctt catcgagccg
     3601 ggtgccagag ttgtggtggc gacgaccggc ggcacctatg tgcggcgcgc cgaatagatg
     3661 ccctatctct cgccggcgct gaacgtgatg acgacggccg cgcgcaaggc ggggcgcggc
     3721 ttgatccgcg atttcggcga gctcgaaaat ctgcaggtct cgctcaaagg cccggccgat
     3781 ttcgtcaccg cggcggacag gcgaaccgag cgcatcctga ttcaggagct ttccagggcg
     3841 cggccgggct accagtttct cgtcgaggag gctggcgcga tcgaagggcc ggaccgatca
     3901 caccgcttca tcatcgaccc gctggacggc accacgaatt tcctgcacgg c
//
LOCUS       DASZMC010000501         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_508399,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000501 DASZMC010000000
VERSION     DASZMC010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508399"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1161)
                     /locus_tag="VG274_10320"
     CDS             complement(256..1161)
                     /locus_tag="VG274_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWE07094.1"
                     /translation="MSIRPVKRVSQSVPTMEGAGVKLRRAFGFGDTSDFDPFLMMDDF
                     RGDRPEDYLAGFPWHPHRGIETITYVLAGEVDHGDSLGNRGKLGSGDVQWMTAGSGIL
                     HQEMPSGDSKGRMHGFQLWANLPAHLKMVKPRYQDVPATAIPEVIDDDGTSVRVICGE
                     FWGKTGPIDGVAAEPRYLDVTVPAGKKKRLKVEDFRQAFAYIFEGSAMFADSSEPFGV
                     LREAEIDGEEVVVRDKVGNRSLVVFGAGDEIVVQAGEEGVRFLLVSGRPLREPIAWHG
                     PIVMNTQDQIRQALSDLRSGAFIKN"
     gene            complement(1328..>2189)
                     /locus_tag="VG274_10325"
     CDS             complement(1328..>2189)
                     /locus_tag="VG274_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWE07095.1"
                     /translation="TDVIVRPEGPLARGSDRRASIAVRPGGSAANQAAWLASFGAGVD
                     FVARVGAADVESETARFKAIGVTPHLAGDRTHETGRLIALIDPDGERSFLTDRGANEA
                     LEARDIPDALIEGAALIHLSGYSFFAPSPRAAVLDVMRRVGNKPTSIDPASAEFLREV
                     GVDNFLAWTRGAAILFPNEEEAAILAGSEDPETQCARLAARCPLVVVKRGAAGAEAAA
                     GEKRWRVNAPKIEAIDTTGAGDAFVAAFLAARLAGADIQPALERAAAAGAAASASVGG
                     RPKAASVQRD"
BASE COUNT          380 a          723 c          713 g          373 t
ORIGIN      
        1 ttctccagca ccacaacatg aacgccggcg cgcgccagca ggaatccgag catcatcccg
       61 gcggggccgc cgcccgcaat gcagcattgc gtcgtgatcg ttgagttcgc catgccgctc
      121 gactccgctg ggcgcgcgag cccgggttcg cgatcgtaga acgcgaaggt taaggcgacg
      181 ttccgactag agcaggagca gtgatgttgc ggttccggtt cgagccacgg gacgggtagc
      241 tcgaaccgat tctcgtcagt tcttgatgaa cgcgccgctg cgcaagtcgc tgagcgcctg
      301 acggatctgg tcttgcgtgt tcatcacgat cggcccgtgc caggcgatcg gctcgcgtaa
      361 gggtcggccg gagacgagga gaaagcgcac gccctcttcg cccgcctgga cgacaatttc
      421 gtcgccggcg ccgaacacga ccagcgaccg gttgccgacc ttgtcgcgca ccaccacctc
      481 ttcgccgtcg atctcagctt cccgcagcac gccgaagggt tcggacgagt cggcgaacat
      541 tgccgagccc tcgaagatat aggcgaaggc ctggcggaaa tcctcgacct tgaggcgctt
      601 cttcttgccc gcgggcacgg tgacgtcgag atagcgcggc tccgcggcga cgccgtcgat
      661 cgggcccgtc ttgccccaga actcgccgca gatgacgcgc acgctcgtgc cgtcatcgtc
      721 gatcacctcg gggatcgccg ttgccggaac gtcctggtag cgcggcttca ccatcttgag
      781 atgcgcgggg aggttcgccc agagctggaa gccgtgcatg cgtcccttgg aatcgccgct
      841 cggcatttcc tgatgcagga tgccactgcc ggcggtcatc cactggacgt cgcccgagcc
      901 gagcttgccg cgattgccga gactgtcgcc gtgatcgacc tcgcccgcca gcacgtaggt
      961 gatggtctcg atgccgcgat ggggatgcca gggaaagccg gcgagatagt cctcgggccg
     1021 atcgccacgg aaatcgtcca tcatgaggaa cggatcgaaa tcggaggtgt cgccgaaccc
     1081 gaacgcccgg cgcagtttta cgcccgcgcc ctccatggtc ggaacggact ggctgacgcg
     1141 cttgacggga cgaatggaca ttttgccctc ctggtctgaa ctgagatggg cttccgaaat
     1201 actgccgctc atccatcgtt gcgactgaag aagttcgacg aattcgctaa tcggaaaccg
     1261 tgttgaaggc gaagataggt atagaaatcg aaggccgcaa gtctatcgaa agatgtgatt
     1321 ggatctatca gtcgcgctga acgctcgctg ccttcggcct cccgccgacg cttgccgaag
     1381 cggcggcgcc ggccgcggcg gcgcgctcga gcgcgggctg gatgtccgcg ccggccagtc
     1441 gtgcggctag aaaggcggcg acgaacgcgt cgcccgcgcc agtcgtgtcg atcgcctcga
     1501 tcttcggtgc gttgacacgc cagcgctttt cacccgcagc cgcctcggcg ccggcagcgc
     1561 cgcgcttcac gacgaccagc gggcagcggg ccgcaagtcg cgcgcactgg gtttctggat
     1621 cctcggagcc tgcgagaatt gcggcttcct cttcgttggg gaacaggatt gccgcacctc
     1681 gcgtccacgc gagaaaattg tccaccccga cctcgcgcag gaattcagcc gacgccggat
     1741 cgatgctggt gggcttgttt cccacgcgcc gcatgacgtc gagaaccgcc gctcgcggcg
     1801 aaggggcgaa gaacgaatag ccagacagat ggatgagcgc cgcgccctcg atcagcgcat
     1861 ccgggatatc gcgcgcttcg agcgcttcgt tggcgccgcg atcggtgagg aagctgcgtt
     1921 cgccgtccgg gtcgatgagc gcgatcagcc gcccggtctc atgggttcga tcaccggcga
     1981 gatgcggtgt gacgccgatg gccttgaacc gcgctgtctc gctttcgaca tcagcggcgc
     2041 cgacgcgggc gacgaaatcg acgcccgcgc caaacgaggc gagccacgcc gcctgattgg
     2101 ctgccgatcc accaggccga accgcaatcg acgcgcgccg gtcggagccg cgcgccagcg
     2161 gcccttccgg ccgcacgatg acgtccgtc
//
LOCUS       DASZMC010000502         4191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1848835,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000502 DASZMC010000000
VERSION     DASZMC010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848835"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VG274_10330"
     CDS             <1..310
                     /locus_tag="VG274_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWE07096.1"
                     /translation="PRLLVTVGSLESHPSATLMDDYRRYYTKHPEAIPGETVDQAIKE
                     LFADAGGYDKAEDTRLMTERLAKSGVKATFVEFDGEEHMSAAINALNRGIPFALRPAP
                     "
     gene            324..1136
                     /locus_tag="VG274_10335"
     CDS             324..1136
                     /locus_tag="VG274_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWE07097.1"
                     /translation="MADKKTAGEILVGIGGWTYEPWRGGMFYPKGHAQARELEYASRA
                     VTSIEINGTFYGSQKPASFIKWAEETPDDFVFSLKAPRFAVNRRVLAEAGPSIEKFFT
                     SGVHHLKSKLGPILWQFAPHKKYEPDDFAAFLNLLPKSVEGLKLRHAIEVRSETFLVP
                     EFIALLRKHNAAIVYAHHDEYAEAADVTADFVYARLQSSAAKVETGYTTADIKKWKAR
                     AEDWAQGKSPKDLPLIAKTAKPATTDVFMYFISGAKERNPAAAQALIKELRK"
     gene            1169..1969
                     /locus_tag="VG274_10340"
     CDS             1169..1969
                     /locus_tag="VG274_10340"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWE07098.1"
                     /translation="MNAPTSEPLVLTSRDGHVLKLTLNRPSARNALSEGLMAALQNAL
                     DAAANDDSRVIVLSAEGPAFCSGHDLKEMTAHRKEADAGEAAFAAIFAQCSKLMQTIV
                     RHPKPIIAQVQGIATAAGCQLVASCDLAVASANARFATPGVNIGLFCSTPMVALSRNV
                     SRKHAMEMLLTGDMISANQAYHMGIVNRVVTHETLDAETMALAQQIASKPRATVKIGK
                     EAFYRQLEMPLSDAYDYASKVMTENMLHAEANEGICAFVEKRDPKWPE"
     gene            1966..2238
                     /locus_tag="VG274_10345"
     CDS             1966..2238
                     /locus_tag="VG274_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07099.1"
                     /translation="MTKDPVLIALLCVFVLTGAISAYYLVLGLREGRIGRKFNFPYRT
                     APLSDGRDPGYAYRSHSPGLFWNEIGMQVLAVAVSIVMIFILLTAP"
     gene            2238..2750
                     /locus_tag="VG274_10350"
     CDS             2238..2750
                     /locus_tag="VG274_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWE07100.1"
                     /translation="MPQYSDDYIKDILRSTKVIAMVGASGNEMRPSFFAMKYLLAKSF
                     IIHPINPSMVGKDILGQKVYASLKDVPAPVDMIDIFRAADAAPEVVREALAEKDRLGI
                     KTIWMQLGVISDEAAKLAADAGLNVVMDRCPKIEWGRMSGEIGWLGVNRKVLDNRKPL
                     LFGKGGSLKY"
     gene            2779..4050
                     /locus_tag="VG274_10355"
     CDS             2779..4050
                     /locus_tag="VG274_10355"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HWE07101.1"
                     /translation="MSEYGFNTLSVHAGAQPDPATGARATPIYQTTAYVFEDADHAAA
                     LFNLQVFGNIYSRIMNPTNSVLEERVAALEGGRAGLAVASGHAAQLIAFHPLMNPGDE
                     FVAARQLYGGSITQFKHSFAKFDWHAKFADCQKPDTFKAAITSKTKALYVESIANPGG
                     IITDLEPIAKIAHDAGVPLIVDNTMATPYLIKPIDWGADIVLHSATKFLGGHGNSMGG
                     VIVDGGKFNWGNGKFPTLSEPCPSYHGMKIWETFGDMAYAIACRVLSLRDLGPSLSPM
                     NAFLILTGCETLPLRMDRHCANALTVAKWLKAHPKVAWVNYAGLEGDASYGLHKTYCP
                     KGAGSIFTFGVKGGYEAGKTLVNSVKLFSHLANIGDTRSLIIHPASTTHRQLESEQRA
                     AAGAGDDVVRLSIGIESVEDIIADLDQALAA"
BASE COUNT          872 a         1319 c         1245 g          755 t
ORIGIN      
        1 gccgcgtctt cttgtaacgg tcggctcgct ggagtcacat ccgagcgcca cgctcatgga
       61 tgactatcgc cgctactaca ccaaacaccc ggaggccatt cccggcgaga ccgttgacca
      121 ggcgatcaag gagttgttcg cggacgcagg tggctacgac aaagcggaag acacgcgcct
      181 tatgaccgag cgcctcgcca aaagcggcgt gaaggcgacg tttgttgaat tcgacggcga
      241 agagcatatg tcagccgcga tcaacgcgct gaaccgtggt attcctttcg cgttgcggcc
      301 agcaccatag ttgcgaggaa cccttggcgg acaagaagac agcaggcgag attctggtcg
      361 gcatcggcgg atggacctat gagccatggc gcggcgggat gttctatccc aagggccacg
      421 cgcaggcgcg cgaactggaa tacgcgagcc gcgcggtcac gagcatcgag atcaacggaa
      481 cgttctatgg ctcgcagaaa cccgcgagct tcatcaaatg ggcggaagag acgccggatg
      541 atttcgtgtt ctctctgaaa gcgccgcgct tcgcggtgaa ccgccgcgtg ttggcagaag
      601 cagggccctc aatcgagaag ttcttcacca gcggcgtgca tcacctgaaa tcgaagctcg
      661 gcccgatcct gtggcagttc gcgccgcaca agaaatatga gcctgacgat ttcgcggcgt
      721 ttctcaatct gcttcccaag tccgtcgagg gtttgaaact ccgtcacgcc atcgaggtgc
      781 gcagcgaaac cttcctggtg ccggagttca tagcgttgct gcgcaagcac aacgccgcca
      841 tcgtctacgc gcatcacgac gaatacgccg aggcagccga tgtgaccgcc gacttcgtct
      901 atgcgcgcct ccagtcttcg gccgcgaagg tcgagaccgg ctacaccacc gccgacatca
      961 agaagtggaa ggcgcgcgct gaagattggg cccagggcaa atcgcccaag gacctgcccc
     1021 tcattgcaaa aaccgcgaag cccgcaacga cagatgtctt tatgtacttt atctccggcg
     1081 ccaaggagcg taaccccgcg gcggcgcagg cgctgatcaa ggaactgcgc aagtagcggg
     1141 ccgcgctgcg acgcattata taggcgctat gaacgcaccc acctccgaac cgctggtcct
     1201 cacgtcacgc gacggccacg tactgaagtt gacgctcaac cgccccagcg cgcgcaacgc
     1261 tctgagcgaa ggcttgatgg ccgcgttgca gaacgcgctt gatgctgcgg cgaacgacga
     1321 ctcgcgcgtg atcgtgctga gcgcggaagg cccggccttc tgctccggcc acgacctcaa
     1381 agagatgacg gcgcaccgca aggaagccga cgccggcgag gccgcgttcg ctgcgatctt
     1441 cgcgcaatgc tccaagctga tgcagaccat cgtgcggcac ccgaagccca tcatcgcgca
     1501 ggtgcagggc atcgcgaccg ctgccggctg ccaactggtt gcaagctgcg atctcgccgt
     1561 cgcttccgcc aatgcgcggt tcgcgacgcc gggcgtaaac atcggcctgt tctgctcgac
     1621 gccgatggtc gccctctccc gcaacgtctc gcgcaagcac gcgatggaga tgctgctgac
     1681 cggcgacatg atcagcgcca accaggccta tcacatggga atcgtcaatc gcgtcgtgac
     1741 gcatgagacg ctcgacgccg agacgatggc gctcgcgcaa cagatcgcat ccaaaccgcg
     1801 cgcgacggtg aagatcggca aggaagcgtt ctatcgccaa ctcgaaatgc cgctgagcga
     1861 cgcctacgac tacgcctcaa aggtcatgac cgagaacatg ctccacgccg aggccaatga
     1921 gggcatctgc gccttcgtcg aaaaacgcga tccgaaatgg ccggagtgac gaaggatcct
     1981 gttctcattg cgctcctgtg cgtgtttgtg ctgacgggag cgatctccgc ctattatctc
     2041 gtgcttggtt tgagagaggg acgcatcgga cgcaagttca actttccgta tcggacggcc
     2101 ccgctttcgg atgggcgcga cccaggctac gcctatcgca gccacagccc gggcctgttc
     2161 tggaatgaaa tcggcatgca agtcttggcc gtagcggtca gcatcgtaat gatattcatc
     2221 ctgctaacgg cgccctgatg ccccaatatt ccgacgacta tatcaaagac atccttcgtt
     2281 cgaccaaggt catcgccatg gtgggcgcga gcggcaatga gatgcggccc tcttttttcg
     2341 cgatgaaata tcttctcgcg aagagcttca tcatccaccc gatcaatcca agtatggtcg
     2401 gcaaggatat tctcggccag aaagtctacg cgtcgctcaa agacgtgccc gcgccggtcg
     2461 atatgatcga catctttcgc gcggccgatg cggcgccgga ggtcgtgcgc gaggcgctgg
     2521 ccgagaaaga tcggctcggc atcaagacga tctggatgca gcttggcgtt atcagcgacg
     2581 aagccgcgaa gcttgcggcc gatgctggcc tcaacgtcgt catggatcgt tgccccaaga
     2641 tcgaatgggg ccgcatgtcc ggcgagatcg gctggcttgg cgtaaaccgc aaggtgctcg
     2701 acaatcgcaa accgcttctc ttcggcaagg gcggcagcct gaaatattga tctgatttcc
     2761 gaaccacgga ggaatgccat gtccgaatac ggcttcaaca cgctgagcgt tcacgcgggc
     2821 gcgcagcccg atcccgccac tggcgcgcgc gcgacgccga tctatcaaac cacggcctat
     2881 gtgtttgagg acgccgatca cgctgccgcg ctgttcaacc tccaggtctt cggcaatatc
     2941 tattcgcgca tcatgaaccc gactaactcg gttcttgagg agcgcgtcgc ggcgctggaa
     3001 ggcggacgcg cggggctcgc tgttgcgtcg ggacatgccg cgcagctcat cgcgttccat
     3061 ccgctgatga accctggcga cgaattcgtc gcggcgcgcc agctctatgg cggctcgatc
     3121 acgcagttca aacattcctt tgcgaagttc gactggcacg caaaattcgc agattgccag
     3181 aagccggaca cgttcaaggc cgcgatcacc tcgaaaacca aggcgctcta tgtcgagagc
     3241 atcgcgaacc ccggcggcat catcaccgac ctcgagccca tcgcgaagat cgcgcatgac
     3301 gcgggtgtgc ccctgatcgt cgacaacacg atggcgacgc cttatctcat caagcccatc
     3361 gattggggcg cggacattgt tctgcactcg gcgacgaaat tcctcggcgg tcacggcaat
     3421 tccatgggcg gcgtcatcgt cgatggcggc aagttcaact ggggcaacgg caaattcccc
     3481 acgctgtcgg agccctgccc gtcctatcac ggcatgaaga tctgggagac gttcggcgat
     3541 atggcctatg ccattgcctg ccgtgttctg tctctgcgcg atctcggtcc gtcattgtcg
     3601 ccaatgaacg cattcctcat tctcactggt tgcgagacgc tgccgctgcg catggatcgc
     3661 cattgcgcca acgcgctgac cgtcgcgaaa tggctgaagg cgcatccgaa ggtcgcgtgg
     3721 gtgaactacg cgggcctcga aggcgacgcg agctatggcc tgcacaaaac atattgcccc
     3781 aaaggcgcgg gcagcatctt caccttcggc gtgaagggtg gctatgaggc cggcaagacg
     3841 ctggtcaaca gcgtgaagct gttcagccat ctcgccaaca tcggcgacac ccgcagcctc
     3901 atcatccacc cggcatcgac cacacaccgt cagctcgaat ccgagcaacg tgcagcggcc
     3961 ggcgcaggtg acgacgttgt gcgcctctcg atcggcatcg aaagcgtcga agacatcatc
     4021 gccgatctgg atcaggctct cgccgcgtaa cgcagttcaa acgcctgcca caccgtcgcg
     4081 cagaagatca gcgcggcggt gagttggtgc agcgcggaca ggccgagcgg cgcttgccat
     4141 agcagcgtgg cgatgccgag gccgacctgg aatacgacga ggctgccgac a
//
LOCUS       DASZMC010000503         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5150001,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000503 DASZMC010000000
VERSION     DASZMC010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5150001"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..955
                     /locus_tag="VG274_10360"
     CDS             326..955
                     /locus_tag="VG274_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07102.1"
                     /translation="MEEARDAADVFFCRTVKPTHDPLDDFLAGEVTFSWAGIGSAVNQ
                     EGVFLMNKYLMLSAAAVLASATGAYAGTFQHSFTFGTAGGSPYCDGGSVYTSGATVWS
                     WQHTNNNCSGGVSYGQGLLEKNGLYGKNANMSDNFFGKNYGIFSEYLNYVLPKKIKNG
                     KPWELYVGINGTTSFFGNSGVLVDAAAAKGRVSTASMLKGLIQTHRASH"
     gene            1194..2864
                     /locus_tag="VG274_10365"
     CDS             1194..2864
                     /locus_tag="VG274_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWE07103.1"
                     /translation="MPSGPPPEISPSSPLDRLLGRAGELIDQGRLAEAIAPMTEAADL
                     LPENASLQANLGILCVEAGRPAKALAPLRRAIALNPRIAVAHWRLGEALHALGDIEGA
                     IGSLEHAVQIRPELSDANLRLALIYVGQGRRRDATECYRRAAQSTPEPAEKQFLEAQA
                     LALEGRESEAEPLLRSALELQPDLPTAHGLLGQVLTASGKFDEAYRHYEAQLARSPRA
                     GLCYYDLVRSRRITDADGDVLKSIDAAIADREVDEINRAVLLLARGKALDDLGRYEEA
                     MQSLDAASELRARAFAPDIESFERQVEEIMALFRTEMLVHRATANHDRTPVLILGMPR
                     SGTTLVEQIVSSHPDVAGGGELIFWRKRLQKALEAGVGGLDSRFPNEAAGEYLRELHT
                     FSNGERVTDKDPFNFLAVGLIHMAFPRAAIIHCRRNPVDAAISIHQTHFSRSTGMPTG
                     GQDLVRYFRANQRLMAHWRRVLPAGRMYEIDYEHLTASPQTEIRALIDHIGLNWDPAC
                     LAPHVNSRLVRTPSGWQVRQSINTGSVDRWRRYEPWLGPLASLREEVA"
     gene            complement(2868..>3263)
                     /locus_tag="VG274_10370"
     CDS             complement(2868..>3263)
                     /locus_tag="VG274_10370"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWE07104.1"
                     /translation="CYKQAFAPGAMYLAYDQTDIGRYYRDYVEVTAHYDRVLPGRVHR
                     VLYENLLREPEAEIRRLLAYCDLPFEEQCLRFHETDRSVRTSSSQQVRQPIQKKKVEA
                     WQQYDTWLQPMKDALGDVLKRYPEAPDFD"
BASE COUNT          630 a         1000 c         1008 g          625 t
ORIGIN      
        1 cgcgggggcc agtgtcgcaa gtacggcggc gagtgtgaca aatcgaacgc gcatgaaacg
       61 ctcccatggg tgatgcgtcg gaagccttgc gcccgcgact gccggatgca agtcgccggg
      121 ccgcacagtc gcgtgattgg caatcacctg acggtgagtt ggtaaataca attccgtgcc
      181 gcccggtttg ccaggcatcc cggggcaggg gattggttcg cccctcggcc tttacctccc
      241 aacgctgcaa cgaccccgcc ggaacgggac agccggcctc gccttcgacg gccgaagata
      301 tcgacgacgg gctggatcgg ctctcatgga agaggcgagg gatgccgccg atgttttttt
      361 ttgccgaacg gtcaaaccga cccatgatcc gcttgacgac tttctcgctg gcgaggtaac
      421 attctcgtgg gctggcatcg ggtcggccgt aaatcaagag ggggtcttcc tcatgaataa
      481 gtatttgatg ttgagcgcag ccgcggttct cgcaagcgcg acgggcgcgt atgccggtac
      541 attccagcat agctttacgt tcggcaccgc tggcggcagt ccctattgtg acggcggcag
      601 cgtctacacc agtggcgcga ccgtttggtc atggcagcac accaacaaca actgcagcgg
      661 tggcgtcagc tacggtcagg gcctgctcga gaagaatggg ctttatggca aaaacgccaa
      721 tatgtccgac aacttctttg gcaaaaacta cggcatcttc agcgaatatc tgaactacgt
      781 cctgccgaag aagatcaaga acggcaagcc gtgggagctg tatgttggca tcaacggaac
      841 gacgtcgttc tttggtaatt ccggcgtgct cgtcgacgcc gctgccgcga agggccgcgt
      901 cagcaccgct tccatgttga agggccttat ccagacgcat cgcgccagcc actaaggcag
      961 gcgattttcg gtttgcaatg cgaagcagcg gcgccccgtg cgccgctgct tttttgcgta
     1021 tgcacagatc cttcatgccc gctttcgccg atttccgaat aatgtctcaa ccttcgacag
     1081 gttcgggatg acgagctcat ggcgagcatg tcgaatcaaa gtccggaacg cttcggaata
     1141 atccttcgaa ttctcctctt tcgggactcc gcggccgccc ctatgttcgc ttcatgccgt
     1201 ctggaccgcc tcccgaaatt tcgccgtcgt cgccattgga ccgccttctt ggccgagctg
     1261 gagaactgat cgatcagggg cgtctcgccg aggcgattgc gccgatgaca gaggccgcag
     1321 acctgctgcc cgaaaatgcc tcgctccagg cgaatctggg cattctatgt gtcgaagcag
     1381 ggcggcccgc gaaggctctg gcgccgctgc gcagggccat cgcgctcaac ccccgaattg
     1441 ctgtcgcgca ttggcggctg ggcgaggcgc tccatgcgct gggcgatatc gaaggcgcaa
     1501 tcgggtccct ggagcacgcc gtgcagatcc ggcccgaatt gagcgatgca aatttgcgcc
     1561 tggcattgat ttacgtagga caggggcgaa ggcgagatgc caccgaatgc tatcgccggg
     1621 ccgcccaatc gacgcccgag ccggcagaaa agcagttcct cgaggcgcag gccctcgcgc
     1681 tcgaaggccg cgaaagcgag gccgagccgc tcttgcgctc ggctctcgag ctccagcccg
     1741 atctcccgac ggcgcacgga cttctcggtc aggtcctgac ggcctcgggg aagtttgacg
     1801 aggcgtatcg gcactacgaa gcgcagctcg cgcgatcgcc ccgcgccggg ctctgttact
     1861 acgatctcgt gcgaagccga aggattacag acgccgatgg agatgtcctg aaatcgatcg
     1921 atgccgcgat tgcggaccgg gaagtcgacg agatcaaccg cgccgttctg ttgctcgcgc
     1981 ggggcaaggc gctcgacgat ctgggccgtt acgaggaggc gatgcaatcg ctggatgcgg
     2041 catcggagct gcgcgcgcgc gccttcgcgc cggacatcga atcattcgag cggcaggtcg
     2101 aagaaatcat ggcgcttttt cgcacggaaa tgctcgtgca ccgtgcaacg gcaaaccatg
     2161 accggacacc ggtgctgata ctgggcatgc cgcgctccgg gacgacgctc gtcgagcaga
     2221 tcgtctccag ccatccggac gtcgcgggcg gcggggaact catcttctgg aggaagcgtc
     2281 tgcaaaaagc gctggaggcg ggagtcggcg gactcgacag ccggttcccg aacgaggccg
     2341 ccggcgaata tctgagagaa ttgcatacat tctcgaatgg cgaacgcgta accgacaagg
     2401 acccgttcaa tttcctcgcc gttggattga ttcacatggc gtttccgcgc gccgcgatta
     2461 ttcactgccg gcgcaacccg gtggacgcgg cgatttcaat ccatcagaca cacttctcca
     2521 gatcgaccgg aatgcccacg ggtggccagg atcttgtccg ctattttcgc gccaatcagc
     2581 ggctcatggc gcattggcga agggtcctgc ccgcgggccg catgtacgag atcgattacg
     2641 agcatctgac cgcctcgccg caaaccgaaa ttcgggcgct catcgaccac atcgggctga
     2701 attgggatcc cgcctgcctt gctccgcacg tcaactcgcg tctggtccgc acgccgagcg
     2761 gctggcaggt gcgtcaatcc atcaacacgg gttccgtcga tcgttggcga agatacgagc
     2821 cctggctcgg cccgctcgcg tcgctgcggg aggaggttgc gtaaccgcta gtcgaagtcc
     2881 ggtgcttcgg gatagcgctt gagcacatca ccgagtgcgt ccttcatcgg ttgaagccac
     2941 gtgtcgtatt gctgccaggc ttccaccttc ttcttctgaa tcggctgacg aacctgctgc
     3001 gagctcgaag tgcgcacgct gcggtcggtt tcgtggaaac gcaggcactg ctcctcgaac
     3061 ggcagatcgc aataggcgag cagacggcga atctccgcct ccggctcgcg cagcaaattc
     3121 tcataaagga cacggtgcac gcggcccgga agcacgcggt cgtaatgcgc cgtcacctcc
     3181 acataatcgc ggtagtagcg cccaatgtcg gtctgatcgt aggcgagata catggcgccg
     3241 ggcgcaaagg cctgcttgta gca
//
LOCUS       DASZMC010000504         6556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4062938,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000504 DASZMC010000000
VERSION     DASZMC010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4062938"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VG274_10375"
     CDS             <1..471
                     /locus_tag="VG274_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HWE07105.1"
                     /translation="VNPKGYVPTLKLDDGATLTEGPAIVQYLADQRPASGLAPANGTF
                     ARYKLQEWLTFINGEIHKAFGPLFGSPGDEVKKDALAKIAKRFGYVEKELAGRPFLTG
                     DNFTIADAYFFVMLTWTQHFHIDLKPFPNITAWFTRVASRPKVHAAMKAEGLVK"
     gene            518..2398
                     /gene="nhaA"
                     /locus_tag="VG274_10380"
     CDS             518..2398
                     /gene="nhaA"
                     /locus_tag="VG274_10380"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HWE07106.1"
                     /translation="MANEAMNGTWLLEPIDGARDHMRGDRAARDAVEVVLYGDYLCPF
                     CRRLRPVFLQLREVLGDCLVYVFRHFPNERAHPGATFMARATEAAAQQDRFWDMHDWI
                     YDSNPAPTQKEAVEFIRSLGLDMDRFERDLQSEEVRLRVEEDVADGKRNGVSGTPTIF
                     IDGLRYDGAWDFYSMLEALEQPVATRIERSARAFASLPASGGIALLLAAAVALVLANT
                     ALAPLYGLFINAPFGIGPPGGMLSLRVADWCSEGLLAVFFLLVGLEIRRELTAGSLTD
                     LRAAALPAIAAVGGVVVPAAIYLLFNPGPTAPGWAVPTATGIAFTLGILALFGQRIPL
                     SLRVFIAAFAVVDDVLSVLTLAIFYPHGFHIAWLGAVAAALAVLYALNRWRVYAAWPY
                     VVSTIGLWIFLHLSGVHAALAGIFLAVLLPARPTPDAGPLLAQAATALATLEHAQTEV
                     SKAGAKKPGVDIEAVWDWAGRNLLAVSDRLLSPSERIEQSVAPWTAYLALPLFAFSTS
                     GVGLSLDLSSPDTTRVLAGVVLGLLAGKPAGVCVASFLAIRSRVAIAPKGIDLRNFLG
                     AACLCGVGDTLSFLMADQAFPHGSDAATAKIGVLAASVLAAALGALILATNKDHNEPV
                     RR"
     gene            complement(2476..3030)
                     /locus_tag="VG274_10385"
     CDS             complement(2476..3030)
                     /locus_tag="VG274_10385"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HWE07107.1"
                     /translation="MSTASLDDSLFHGRVRASSRGIFWLGLAMVVLGIAAIVFPMFST
                     LVAALLVGWVLLISGGFTFAGSFSIHGTGPFFGALLLGLLSIAGGVFLLFNPLAGAVA
                     LTLIVGVLFMFQGAFEIFFAIETRPHAGWVGMLISGIASVVMAVLIAAGWPAISVIVL
                     GILLGVNFLTTGLGYIFVSNSLKP"
     gene            complement(3505..4017)
                     /locus_tag="VG274_10390"
     CDS             complement(3505..4017)
                     /locus_tag="VG274_10390"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HWE07108.1"
                     /translation="MFRAVSSDIGFLMNLRVIIETFRRNVTDHYFDLQGRVSRQEFWR
                     FVLVCVVIYFIAFIVEEIIRRRLLTPVIGLALLLPIAGLGARRLQDTGRNGMLIWAYS
                     IPTAILELVSLAGAYGPNGMSVSLYSIFMLACLVWLVAAIAFAWLWAQPGTAGINAYG
                     PDPKAAIAAA"
     gene            complement(4092..4529)
                     /locus_tag="VG274_10395"
     CDS             complement(4092..4529)
                     /locus_tag="VG274_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWE07109.1"
                     /translation="MSTDSRVTVRVAHRYSAPAERVFDAWLDPKTADKWLFATPTGQM
                     TRMEIDPRQGGKFTIVRRDGEEFEHVGDYVEIDRPRRLVFTFGVPRLSPEFTRVAIEI
                     FPAEPGCELTLAQDGVLAGYAEPSVKGWTMILDSLERALGDTA"
     gene            complement(4771..5961)
                     /locus_tag="VG274_10400"
     CDS             complement(4771..5961)
                     /locus_tag="VG274_10400"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWE07110.1"
                     /translation="MPGKRRGSGRTFVVVAVVVAAVAAAAVIFSTRFPRWYAVRPYPG
                     MPPVVDRSNIYGEAGRNRFSRAVAGALERIYVPNIRSGDVYVIDPRTLRVVDRFKTGR
                     NAQHVVPSWDLRTLWVTGSATTGRHDGSLTPIDPKTGKPGRIIPVPDAYNMYFTPDGR
                     AAIVVAEAQRRLEFRDPRDMAFEDALPVRECAGINHADFAADGSYAIFTCEFGGGLVK
                     LDLLARRIVGHLNLSRGRMPQDIRLAPDGSIFYVADMMADGVFLIDGERFAEVGFIPT
                     GIGAHGLVVSRDGRKLYVSNRGAHSALGPPHGPGSVSVIDFATRRVERTWPIPGGGSP
                     DMGNVSADGKHLWLAGRFDNVAYDIDTATGAVRTIPVGHEPHGLAVWPQPGRYCLGHT
                     GIMR"
     gene            6182..>6556
                     /locus_tag="VG274_10405"
     CDS             6182..>6556
                     /locus_tag="VG274_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07111.1"
                     /translation="MGKNIVQLGTRAAQAGAVALYILAGNPASAVGKYTRIDPQGSKY
                     TSPTGINRPGAIAGTYSDASSLLHGFVRTGDGTITSFDPTGSSGTFPTAIDDGGAIAG
                     YCSDASHVTHGFLRKPDGTIKSF"
BASE COUNT         1201 a         2107 c         2057 g         1191 t
ORIGIN      
        1 gtaaacccca agggctatgt gcccacgctg aagctcgacg acggcgccac gctgacggaa
       61 ggtcctgcga ttgttcagta tctcgccgat caacggcccg ccagcggcct cgcgcccgcc
      121 aacggcacct tcgcgcgtta caagcttcag gagtggctca ccttcatcaa tggcgagata
      181 cacaaggctt tcggaccgct gtttggctcg cctggcgacg aggtgaagaa ggacgccctg
      241 gcgaaaattg ccaagcgctt cggctatgtg gaaaaggaac tcgccggcag gccgtttctc
      301 accggtgaca attttacgat cgccgacgcc tattttttcg tgatgctgac ctggacccag
      361 catttccaca tcgatctcaa gcccttcccg aacatcaccg cctggttcac ccgcgtcgcc
      421 tcacgcccca aagtccacgc cgcgatgaaa gccgaaggtc tggtgaagta ggtacgcgtt
      481 tcctccccca tcgtggggga ggaaatgagc atgagcgatg gcgaacgagg cgatgaacgg
      541 cacctggctc ttggagccca tcgacggggc gcgcgaccac atgcgcggag accgcgcggc
      601 gcgcgatgcc gtcgaggtcg tgctgtacgg cgattatctg tgcccgtttt gccgacggct
      661 caggccggtg tttctccagt tgcgggaagt gctgggcgat tgcctcgtct atgtgttccg
      721 gcattttccg aatgagcgcg cccatcccgg tgcgaccttc atggcgcggg cgacggaggc
      781 cgcagcgcag caggaccggt tctgggacat gcatgactgg atttacgaca gcaatccggc
      841 gccgacgcag aaggaagccg tcgaattcat tcgttcgctg ggcctcgaca tggatcgctt
      901 cgagcgtgac ctgcagtccg aagaggttcg tctgcgcgtc gaagaggatg tggccgatgg
      961 caaacgcaat ggcgtttcgg ggacgccgac catcttcatc gacgggctgc gttacgacgg
     1021 cgcctgggat ttctactcca tgctcgaggc gctcgaacag ccggtcgcca cgcgcatcga
     1081 gcgctcggcg cgcgccttcg ccagcctgcc ggcttcggga ggcattgcac tgctgctcgc
     1141 ggcggccgtg gcgctcgttc ttgcgaacac cgcgctcgcg cccctctacg gcctcttcat
     1201 caatgcgccg tttggaatcg gcccgccggg cggcatgctg tccctgcgag tcgccgattg
     1261 gtgctccgaa gggctgctcg ccgttttctt tctgcttgta ggtctggaaa taaggcgcga
     1321 gctgacagcg ggctcgctca cggatctccg ggcggccgcg cttccggcaa ttgccgcggt
     1381 gggcggcgtg gtggtaccgg cggcaatcta tctgctcttc aatcccggac caacggcgcc
     1441 gggctgggcg gtgcccactg caaccggaat cgcgttcacg cttggaatcc tcgccctctt
     1501 cggacagcgc attccgctct ccctgcgcgt gttcatcgcg gccttcgcgg tcgtcgatga
     1561 tgttctttcg gtgctcacgc tcgcgatctt ctacccgcac ggatttcaca tcgcgtggct
     1621 cggcgccgtc gcagcagcgc tggcggtcct ttatgcgctg aatcgctggc gcgtgtatgc
     1681 ggcgtggccc tatgtcgttt ccaccatcgg actgtggatt ttccttcacc tctcgggagt
     1741 tcatgccgcg ctcgccggta ttttcctggc cgtcctcctg cccgcgcggc ccacgcctga
     1801 cgcggggcct ttgctggcgc aggcagccac cgcgctcgcc acgctcgaac acgcccagac
     1861 ggaggtgtcg aaggcgggcg cgaagaaacc gggggttgat atcgaggcgg tgtgggactg
     1921 ggcgggccgc aatctcctgg ccgtcagcga ccgtttgctc tcgccctccg agcgcatcga
     1981 acaatcggtt gctccctgga ccgcctatct cgctttgccg ctcttcgcct tctcgacatc
     2041 cggtgtcggt ctcagtctgg atctgtcgtc tccggacacc acaagagttc tcgccggagt
     2101 ggtgctgggg ctcctggccg gcaaacccgc aggcgtttgt gtggcgtcgt ttctggcgat
     2161 caggtcgcgc gtcgccatcg cgcccaaggg catcgatctg cgcaactttc ttggcgccgc
     2221 ctgtctgtgc ggcgtcggcg atacgctctc cttcctgatg gcggaccagg cgttcccgca
     2281 cgggtccgac gccgccacgg caaaaatcgg cgtgctcgcc gcctcggtcc tcgccgccgc
     2341 cctcggcgcg ctgatcctcg ctacaaacaa ggatcacaat gaaccagtga ggcggtgaga
     2401 aggagatcgc gcgcctttgg cgcgcaattg ttctcaccgc ctcactgtct cattgtgaat
     2461 ctttctgctg gttcgtcacg gcttcaggga attcgagacg aagatgtagc cgaggccggt
     2521 ggtgaggaaa ttcacgccga gcaggatgcc cagcacgatc accgagatcg caggccagcc
     2581 cgccgcgatg agcactgcca tcacgacgct cgcaatgccc gaaatcagca tgcccaccca
     2641 gcccgcatgg gggcgcgtct cgatggcgaa gaatatctcg aacgcgccct ggaacatgaa
     2701 gagcacgccc acgatcagcg tcagcgcaac cgcgccggca agcggattga agagcaggaa
     2761 cacgccgccg gcgatcgaga gcaggccgag cagaagcgcc ccgaagaacg gcccggtgcc
     2821 gtggatcgaa aacgaaccgg caaaggtgaa tccgcccgag atcagcaaca cccagccgac
     2881 gagaagcgcc gcaaccagag tggagaacat cggaaagacg atggccgcga tgccaagaac
     2941 aaccatcgcc aggccgagcc agaaaatccc gcgcgacgat gcgcgcacgc gcccatgaaa
     3001 caggctgtcg tccaaagatg cagtgctcat tgccggctcc ccctgacgag acggccccgg
     3061 atggcagccg tttcggacgc cagcgtaatc cacgccgctc cccgctgcaa tcgagggcag
     3121 gtatttctgc ccgtcacaac cgaatgtaga ttcccgcgcg cccgcgagtg aatgatctgg
     3181 aatccggcca gggttctgtt caaagcgcgc aacgctcgcg agctttccga tatcaaatgc
     3241 gaacccgatc atatcgtcgc gaaaacgctg tcaagcgtct catacgaaac cgcaactcgc
     3301 gcgtttgtga ttcgagcgcc ggcggagatc atgcatgttg gacaatctgt cttttggcaa
     3361 gcgcatcgta tttggactcg tatttggaat catgttctgg ggtggggtcg ccctgtcgag
     3421 cgcttatcac ctgtaaagcc aggcagacgc gaacctgctt cgcgccgcga tggcgcattg
     3481 gtctttcaat caattccgaa gacttcacgc ggcagcaatt gcggccttcg ggtcgggacc
     3541 gtaggcgttg atgcctgccg tgcccggctg cgcccagagc caggcgaacg caattgctgc
     3601 gaccagccag acgagacatg ccagcatgaa gatggaatag agcgagaccg acattccgtt
     3661 cggcccgtac gctcccgcga gcgaaaccag ttccagaatt gcggtgggaa tcgaataggc
     3721 ccagatgagc atgccattcc tgccggtgtc ctgcagacgc ctcgcgccga ggcccgcgat
     3781 gggaagcagc agagccaggc ctatcaccgg cgtcaggagt cggcggcgaa tgatctcttc
     3841 gacaatgaag gcgataaaat agatcaccac gcagacaagc acgaagcgcc agaactcctg
     3901 ccgcgaaacg cggccctgca ggtcaaaata atggtcggtg acgttgcggc ggaacgtttc
     3961 gatgatgacg cgcaggttca tgagaaagcc tatgtcggac gatacggcgc ggaacaaata
     4021 ttcatcccgt gcaagcccca aggggtgtgc gcggacaatg tacaggcatc cgagggtttt
     4081 gggaggaggg gtcaggccgt gtcgcccagc gcgcgctcga gactgtcgag gatcatcgtc
     4141 cagcctttca cgctgggctc ggcatagccg gccaacacgc cgtcctgggc gagggtcagt
     4201 tcgcaaccag gctccgcggg aaagatttcg atcgcgacgc gcgtaaattc gggggacagc
     4261 ctggggaccc cgaacgtgaa tacgaggcgg cgcggccgat cgatctcgac atagtcgccg
     4321 acatgctcga actcctcacc gtcgcggcgg acgatcgtga attttcctcc ttggcgcgga
     4381 tcgatctcca tgcgggtcat ctgacccgtc ggcgtcgcaa acagccattt atccgccgtc
     4441 ttcggatcga gccacgcgtc gaacacccgc tccgccggcg ccgaatagcg atgcgcgacc
     4501 cgcacggtga ccctggagtc tgtggacatc attttcttcc tttgcaggtg cgagaggagc
     4561 gcccctcttt cctcccccaa cgtgggggag gtgtcgagcg cagcgagacg gagggggacg
     4621 tcgcgcgcgc agacacatcc ccctccggcc cttcgggcca cctcccccac gttgggcgag
     4681 gaaatgtgca atttggtccg cgtcgtcggt gttccccgcg gctctgcgag ggcctctcgc
     4741 cgacttgcac tctcgcaccg ttagacgcgt tcaccgcatg atgccggtgt ggccgaggca
     4801 gtagcggccg ggttgcggcc acactgcgag gccgtgcggt tcatggccta cggggatcgt
     4861 ccgcacggca ccggtcgcag tgtcgatgtc atacgcgaca ttgtcgaagc ggccggccag
     4921 ccacaggtgc tttccgtcgg cgctgacatt gcccatgtcg gggctgccgc ctcctggaat
     4981 gggccaggtg cgttccaccc gccgcgtggc gaaatcgatg accgacacgc tgccgggacc
     5041 gtgtggcggg ccgagcgcgc tgtgcgcgcc gcgattggaa acataaagct tcctgccatc
     5101 gcggctgacc accagcccgt gggcgccgat gccggtggga atgaatccca cctccgcaaa
     5161 tcgctcgcca tcgatcagga acacgccgtc ggccatcatg tcggcgacat agaagatact
     5221 gccgtcgggt gcgagacgga tgtcctgggg catccggcca cgcgacaggt tgaggtggcc
     5281 cacgatgcgg cgtgcgagga gatcgagctt gaccagcccg ccgccgaatt cgcaggtgaa
     5341 gatcgcgtag cttccgtccg cggcgaaatc ggcatgattg atgccggcgc attcgcgcac
     5401 cggcagcgca tcctcgaacg ccatgtcgcg cggatcgcgg aactcgagcc gccgctgcgc
     5461 ttcggccacc acgatcgccg cccgcccgtc cggcgtgaaa tacatgttat aggcgtcggg
     5521 aaccgggatg atgcgccccg gcttgccggt cttcggatct atcggcgtaa ggctgccgtc
     5581 gtggcggccc gtcgtcgcgc tgccggtcac ccaaagcgtg cgcaaatccc atgacggcac
     5641 aacatgctgg gcgttgcggc ccgttttgaa ccggtcgacc acgcgaagcg tgcgcgggtc
     5701 gataacatag acatcacccg aacggatatt ggggacatag atgcgctcga gtgcgccggc
     5761 gaccgcgcgg ctgaagcgat tgcgtcctgc ctcgccatag atgttgctgc ggtcgacaac
     5821 cggcggcatg ccgggatagg gccgcacggc ataccagcgc gggaagcgag tcgagaaaat
     5881 caccgctgcc gccgcaaccg ccgccaccac cactgcaacg acgacgaacg ttctccccga
     5941 tccgcggcgc tttccaggca cgttcgttta ccctcgctcg ggcttccgcg ggtccgtgtg
     6001 cgatatcgac gggcgccaag tggcaacgtt tctgtccggc acgcggtttc gcatgcttgc
     6061 ggcggtttcg tgctcaccgc catggttcga caagctcacc atgaggatct catcctgagc
     6121 ttgtcgaagg atgtggtccg ggacaggtgt cgcggtggca ggcgcgagga agcggaacat
     6181 cgtgggcaaa aatatcgtgc agctcggaac gcgcgcagcg caggctggcg cagtagcgct
     6241 ttacatcctg gcgggcaacc cggccagcgc cgtgggcaaa tacacgagga tcgatcctca
     6301 aggatcaaag tacacgtccc caaccggtat caaccggcca ggtgcgatcg cgggaaccta
     6361 ctcggatgcc agcagcctct tgcatggctt tgtgcgcacg ggcgacggga cgatcacgag
     6421 cttcgacccg acgggatcga gcggcacctt tcccaccgcc atcgacgatg gcggtgctat
     6481 cgccggttat tgcagcgacg cgagccatgt aacgcacggc tttctccgca agccggacgg
     6541 aacgatcaag agcttc
//
LOCUS       DASZMC010000505         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2412485,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000505 DASZMC010000000
VERSION     DASZMC010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412485"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1605)
                     /locus_tag="VG274_10410"
     CDS             complement(1..1605)
                     /locus_tag="VG274_10410"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001968.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWE07112.1"
                     /translation="MASEFAPELARAAHAWPFEEARKVLARLERWQNRGLAKPGAITF
                     ETGYGPSGLPHIGTFGEVARTSWVRRAFALMSGIPTRLCSFSDDMDGLRKVPGNVPNQ
                     DMLREHLGLPLTSIPDPFGTHSSYGAHNNARLRAFLDSFGFDYEFLSATECYKSGRFD
                     GALMTVLANYEKVLDIVLPTLGPERRATYSPFLPICPRSGRVLQVPILEWNAARGTVV
                     YEDEGRRSEAPVTGGHCKLQWKADWAMRWLALGVDYEMSGKDLISSVELSSKILRALG
                     GEPPEGFNYELFLDENGERISKSRGNGLSIDEWLAYGPQESLALFMYQKPRTAKRLYF
                     DVIPKAVDEYIELLRQYWNLTDDSQRIECAVWHIHAGDPPPDSYPVSFALLLNLVSAS
                     NAQDRDVLWGFIRAYAPGADPNTDPALDRLVGHALRYYEDRVKPQKRYRVPNEKERAA
                     LEDLARVLQSMPEERNGEAVQNLVYEVGKRHGFEPLREWFRALYEILFGQSQGPRFGS
                     FAALFGCGPTAALIHRAVAGELTGVA"
     gene            1733..>2622
                     /locus_tag="VG274_10415"
     CDS             1733..>2622
                     /locus_tag="VG274_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07113.1"
                     /translation="MDEIELKELADVPALVPAPGGAHIALAGGACRETGWDEARTLFR
                     SGAVLIANTAFVAGRLGMHPLKPVFDLVELFAFVRPATPFVPSALGVARAAGVSLPSS
                     RDASVRALREAAGVLVDQLRKFPEPERVRLQPLADLLARAGWRWGPLVVGAVGELKRA
                     APPLAALAAWQSLPQWEDDAPPPKPSSIPVGSDETRARLARLIGASGETRLEQSAYAD
                     AATHAFASRERAGAPRVALLEAGTGVGKTLGYLAPASLWAEKNGPGLWLSTYTRNLQR
                     QLVQEIAHLWPDPIERAEKAV"
BASE COUNT          491 a          835 c          805 g          491 t
ORIGIN      
        1 tcatgcgacg cctgtgagct cgcccgcaac ggcacggtgg atcagcgcgg cagtcggtcc
       61 gcatccgaac agcgccgcga atgagccgaa gcgcggaccc tggctttggc cgaaaagaat
      121 ttcgtagagc gccctgaacc attcccgcaa cggctcaaat ccgtgccgct tgccgacttc
      181 atagaccaaa ttctgcaccg cttcgccatt gcgctcctcc ggcatcgatt gaagcacgcg
      241 ggcgaggtct tccagcgcgg cgcgttcctt ttcattcggc acacggtagc gcttctgcgg
      301 cttcacccga tcctcgtagt agcgaagcgc atgtccgacc aggcgatcga gcgccggatc
      361 ggtattggga tcggcgccgg gcgcgtacgc acggatgaac ccccacagca catcgcggtc
      421 ttgcgcgttc gacgcactga cgagattgag caggagggca aaacttaccg gataggaatc
      481 cggcggcgga tcgccagcat gaatgtgcca cacggcgcat tcgatccgct gcgaatcgtc
      541 ggttagattc caatattgcc ttagaagctc gatgtactcg tccaccgcct tggggatcac
      601 gtcaaaatag aggcgctttg cagtgcgcgg cttctgatac atgaaaagcg cgaggctttc
      661 ctgtggccca taggcgagcc attcgtcgat ggagaggcca ttgccgcgcg atttggagat
      721 gcgttcgcca ttttcgtcga ggaaaagctc gtagttgaaa ccctccggcg gctcgccgcc
      781 aagcgcccgg aggattttcg acgacaattc cacggaggaa atcaggtcct tgccggacat
      841 ctcgtaatcg acgccgagcg ccagccagcg catcgcccag tccgccttcc attgcagctt
      901 gcagtgaccg ccggtgaccg gcgcctcgct gcggcggccc tcgtcctcat agacgactgt
      961 accccgcgcg gcattccatt cgaggatcgg cacttgcagc acccggcccg agcgcgggca
     1021 gatcggcaag aacggcgaat aggtcgcgcg ccgctccggg ccaagcgtgg gcagcacgat
     1081 atcgagcacc ttttcataat tcgcgagcac ggtcatgagg gcgccgtcga accggccgct
     1141 cttgtagcat tccgtcgcgc tcagaaattc atagtcgaat ccaaagctgt cgagaaaggc
     1201 ccgaagccgc gcattgttgt gcgcgccgta gcttgaatgc gttccaaacg gatcgggtat
     1261 cgatgtaagg gggagcccca gatgttcgcg cagcatgtcc tggttgggaa cgtttcccgg
     1321 caccttgcgc aggccatcca tgtcatccga aaacgaacag agccttgtcg gaattccgct
     1381 catcagcgcg aaagcccgcc tgacccagct tgtccgcgcg acctcgccga atgtaccgat
     1441 atgtggcaga cctgacggtc cgtaacccgt ttcgaacgta atcgccccgg gttttgcaag
     1501 cccgcggttc tgccagcgct cgagacgcgc cagaaccttc cgcgcctctt caaagggcca
     1561 cgcatgagcc gcgcgggcga gctcaggcgc aaattcagaa gccattggaa cagctgtgcc
     1621 ttgagaattt cgacgagcgc gaaagctagg ctttgccgtg ggattccgtc aatgtgcgtt
     1681 gcgcaaagca atgaaaccgt caaatgcagt tacggtttgc gcgcccattg caatggatga
     1741 aatcgaatta aaggaacttg ccgacgttcc cgcgctcgtt ccagcgccgg gaggcgcaca
     1801 tattgcgctt gccggggggg cttgccgcga gacgggttgg gacgaggcgc gcacgctctt
     1861 tcgttcgggc gccgttctga ttgcgaacac ggccttcgtc gccggcagac tcggaatgca
     1921 ccctctcaag ccggtgttcg atctggtcga attgttcgca ttcgtccggc cggcaacgcc
     1981 ctttgttccg agcgcactgg gtgttgcccg cgcagccggt gtctccctgc cttcatctcg
     2041 cgatgcttca gtgcgggcgc tgcgtgaagc ggcgggcgtt ctcgtcgatc aactgcgcaa
     2101 atttcccgag cccgagcgcg tgcgtcttca acctctggcg gatttgctcg cgcgagcagg
     2161 gtggcgatgg ggaccgctcg ttgtcggcgc ggtcggcgag ctgaaacgtg cagccccgcc
     2221 gctcgcggct ctggccgcct ggcagagcct tccccaatgg gaagatgacg cgccgccgcc
     2281 caagccctct tcgattccgg tagggagcga cgaaacgcgt gcgcgcctcg cgcgtctgat
     2341 cggcgcctcg ggagaaacca ggctcgaaca gagcgcctat gcggacgcgg ccacacatgc
     2401 tttcgcttcg cgcgagcgcg ccggagctcc acgcgttgca ctgctggaag cgggcacggg
     2461 cgtggggaaa acccttggct acctcgcacc ggccagcctg tgggcggaaa aaaacggccc
     2521 cggtctgtgg ctctccacct acacccgcaa tctgcagcgc caactggtgc aggagatcgc
     2581 ccatctctgg cccgatccaa tcgagcgcgc ggagaaggcg gt
//
LOCUS       DASZMC010000506         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4341416,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000506 DASZMC010000000
VERSION     DASZMC010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4341416"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VG274_10420"
     CDS             <1..295
                     /locus_tag="VG274_10420"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWE07114.1"
                     /translation="GGSSGLSIGHVSPEAAEGGLIGLVEEGDVIEFDIPNRKVDLKVA
                     DAELKRRREAMVAKGNAAWKPAAPRKRAVSTALKAYAAFASSAAKGAVRILPE"
     gene            complement(320..532)
                     /locus_tag="VG274_10425"
     CDS             complement(320..532)
                     /locus_tag="VG274_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07115.1"
                     /translation="MSRFVVQFMKDVLGGNGREREVCQGALEIDASSEGQATEIAKLK
                     FCEQQALCDWSLHADRIRIDAADLHA"
     gene            complement(1145..>1906)
                     /locus_tag="VG274_10430"
     CDS             complement(1145..>1906)
                     /locus_tag="VG274_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HWE07116.1"
                     /translation="CTAAGVLVVNQSGGNANSVAEHALGMMLTLSKRIIQSDRRLRRE
                     PNVNRNDLIGNELKEKTVGIVGLGNVGRRIAELCRGLLHMKVIAYDPYLSAEEMAKRG
                     GEKVELDDLLRRADFVSISCPLDYKSRGMISTREFALMQPHAYFVTTARGFIHDEKAL
                     EEALREKRIAGAGLDVWDKEPPPPDHPLLQFDNVLASPHTAGVTREARINMGKIAAEQ
                     ILDALDGKRPPRIINPEVWPDYAKRFEKAFGFVPK"
BASE COUNT          335 a          614 c          618 g          339 t
ORIGIN      
        1 cggcggatcg tcaggcctgt cgatcggtca cgtctcgccg gaagccgccg aagggggcct
       61 gatcggcctt gtcgaggagg gcgatgtgat cgaattcgac atccccaacc gcaaggtcga
      121 tctcaaggtc gcggatgccg agctcaagcg ccgccgcgag gcgatggtcg cgaagggcaa
      181 cgctgcgtgg aagccggcgg cgccgcgcaa gcgcgccgtc tcgaccgcgc tgaaagccta
      241 tgcggccttc gccagcagcg cggccaaggg cgcggtgcgc atcctgccgg agtgatcggc
      301 gttatcaggt cacgccagat caggcgtgaa ggtcggccgc atcgatccgg atgcggtcgg
      361 catgcaacga ccaatcgcac agcgcctgct gctcgcagaa tttgagtttg gcgatttcgg
      421 tggcctggcc ttcggacgag gcgtcgattt cgagcgcccc ctggcagacc tcgcgctcgc
      481 ggccgttgcc gccgagcacg tccttcatga actggacgac aaagcgcgac atggaatcct
      541 ccccgcacgc cgtagcattg cacggactca cattagcact tgcgccagaa cgattggatt
      601 aaatcagcgc gaacgatgcg cgaaaggctc gagcaggaca gctgtcctta cgcgccagcc
      661 tgaggcaggc tggcggcggc gatcgcgacg atctgcgcaa tgcggcggcc gatcctggcc
      721 ggagcagcgc ggagaaatgt ctggacctgt cgcatgacac attttccttc ggtggccaga
      781 gcgcccggcc acgatgggaa gatggcgata tcaggctaag tcgtcgatat ctcagggaaa
      841 ataacaacgg acgcgtcgcc aagcggtggt ttggcagtgg gtttgttgct tcattcgagc
      901 ctgcggcgaa ggagattttc tccgttcgct tccagcccca cgacgcactc cacctctccc
      961 gcttgcgggg gaggtcgacg cgctcggcaa cgcacttgcg tgccggagcg cggcgggtgg
     1021 aggctctctc cgctcaaagg ctccgactca tggaggtacc cccaccccgc cctcccccgc
     1081 acgcgggaga gggagcccac cgtccgcgca tgcggcacga ttagaatcgg ataccgcagc
     1141 ctccctactt cggcacgaac ccgaacgctt tctcgaaccg cttggcgtag tcaggccaga
     1201 cctcgggatt gatgatccgc ggcggtcgct tgccgtccag cgcgtcgagg atttgctcgg
     1261 cggcgatctt gcccatgttg atgcgggctt cgcgggtgac gccggcggtg tgcgggctcg
     1321 ccagcacatt gtcgaactgc agcagcggat gatccggcgg cggcggctcc ttgtcccaga
     1381 cgtcgaggcc ggcgccggcg atgcgttttt cacgcaatgc ttcctcaagc gccttctcgt
     1441 catggatgaa gccgcgcgcg gtggtgacga aataggcgtg cggctgcatt aacgcgaatt
     1501 cgcgggtgct aatcatgcct cggctcttgt agtccagcgg gcacgagatc gagacgaaat
     1561 ccgcacgccg caggagatcg tcgagctcga ccttctcccc gccccgcttc gccatctcct
     1621 ccgccgaaag atacggatcg taggcgatca ccttcatgtg cagcaggcca cggcacagct
     1681 cggcgatacg acgtccgaca ttgccgaggc cgacgatgcc gacggtcttc tccttcagct
     1741 cgttgccgat caaatcgttg cggttgacgt tgggctcgcg ccgcagccgg cggtccgact
     1801 ggatgatccg cttcgacagc gtcagcatca tgccgagtgc gtgctcggcg accgaattgg
     1861 cgttgccgcc ggactgattg acgacaagca cgcccgccgc ggtgca
//
LOCUS       DASZMC010000507         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2413405,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000507 DASZMC010000000
VERSION     DASZMC010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413405"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="VG274_10435"
     CDS             <1..1804
                     /locus_tag="VG274_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE07117.1"
                     /translation="ATREAWTPLPNENPFVSFLRHSPQHYGLVQGTVALAFADHLFET
                     VPPAMIGAGVDVIARGNASMIARLGFRTIASRIGFLGAASTALWLLDACVGYFHSTTS
                     ERLALAVENDLRNEAYRHLERLDAAVIESRPVSDWIVLIDDDIARIGRFIGVDIDPII
                     TIAANSLIVLTTFLSFSPVLALAHLALAPFLFFLSRAILSRLVERYKTMREYETRLTR
                     VLHGNVAGITTIASYTQQEYEANRVAQAAEEWGEAMTRFNNLSAFYGPAIRVTGGIGF
                     LSTLLWGSVLAARGDVSTGAYSQMSSASLRLLNALGRLGGAIEHYERANVSMARVVRM
                     LERRPEVTGGAGFLSDTAARGDIVFEAVRFGYNSENPVLRDVDIRFPGGKISALVGST
                     GAGKTTVLKLLLRFYESQSGSIRIGGANIRDVELNSLRGQMALVPQNTFLFGGTIREN
                     IAYSRRNASDAEVREAARIAGANAFISELPDGYETIITEGGFQLSGGQQQRIAIARAV
                     LSKRSVLLFDEATSAIDFETEAAIQHSLREAAGDQTIVLIAHRLSTVRNADIIYVIDD
                     GRVVEEGRHHDLIRANGLYASLWRIQTGETASRS"
     gene            complement(1813..2838)
                     /locus_tag="VG274_10440"
     CDS             complement(1813..2838)
                     /locus_tag="VG274_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07118.1"
                     /translation="MSQTNYVSELISGQSILYLSERDVLDLDVYDFKRALPLMVDVLR
                     IHHAGHTRMPRSEYLGYNGRSAYDRMISLLGYVGNGREIAGAKLINSSTLNVRRNLPR
                     ASGILILCDTETQRIYCMISASHVSAVRTAAITGLAISLLRPPRVEKVALIGSGALAW
                     VHVRMWSQIFPEPAAVFALFDINRSRAEALVKHCAALGVSAHVCSAAGEAIAGADIVV
                     PATTAGAPWIKGEWLKPGSLYAAVSLLDAELDVFTQANQVVVDDLELCTQENRPLHVL
                     QQRGRLRDLRVTTLGALVAGDAQIAVSGSDRIVFNPMGTVMTDLVLGASLFERARANG
                     AGQVLPA"
BASE COUNT          569 a          925 c          923 g          566 t
ORIGIN      
        1 cgccacgaga gaggcctgga cgcctcttcc aaacgaaaat ccgttcgtca gtttcctgag
       61 acacagcccg cagcactatg gcctggttca aggcacggta gcgctggcgt ttgccgacca
      121 tctgtttgaa accgtgccgc ccgccatgat cggcgcgggc gtcgacgtaa tcgcgcgcgg
      181 aaacgcctcg atgatcgcga ggctcggttt ccgcaccatt gcctcgcgga tcggatttct
      241 gggcgcggcc agcaccgctc tctggctgct cgatgcgtgc gtcggctatt tccactcgac
      301 cacatccgaa cgcctggctc tcgcggtcga gaacgatctc cgcaacgaag cctaccgaca
      361 cctggagcgg ctcgacgcgg cggtaatcga gagccggccc gtttcggact ggattgtgct
      421 gatcgacgat gacatcgcgc gtatcggcag gttcatcggc gtcgatatcg atccgattat
      481 taccattgca gcgaactccc tcatcgttct caccacgttc ctttcgtttt cgccggtgct
      541 ggcgctggcg catctggcgc tcgcgccgtt cctgtttttc ctgtcgcgag caatcctcag
      601 ccggctcgtc gagcgctaca aaacaatgcg cgaatacgag acccggctta cacgggttct
      661 gcacggcaac gtggccggaa ttacgacgat cgcgagttac acgcaacaag agtacgaggc
      721 gaaccgggta gcgcaggccg cggaagagtg gggcgaggca atgacgcggt tcaacaatct
      781 gagcgccttt tacggaccgg cgatccgcgt cacgggaggc atcggctttc tgagtacttt
      841 gctctgggga tccgttctgg cggcgcgcgg cgacgtctcg accggcgcgt acagccagat
      901 gagctccgcc tcactgcggc ttctgaacgc gctcggccgt ttgggcggcg cgattgaaca
      961 ttatgagcgc gcaaacgttt ccatggcccg tgtagtccgt atgctcgagc gccgccccga
     1021 ggtgacgggc ggcgccggct ttcttagcga taccgcggcg cggggggaca tcgtgttcga
     1081 ggccgttcga ttcggctaca attccgaaaa tcccgtgttg cgcgacgtcg atatccggtt
     1141 cccgggcgga aagatatcgg cgctggtggg ctcaaccggc gccgggaaga cgacggtgct
     1201 gaagctgctg cttcgcttct atgagagcca aagcggatcg atccgcatcg gcggcgccaa
     1261 catccgcgac gtcgagttga attctctgcg cggacagatg gccctggtac cccagaacac
     1321 gttcctgttc ggcgggacaa tacgcgagaa cattgcttat tcgaggcgca acgcctcaga
     1381 tgcggaagtc cgcgaagcag cgcgcatcgc cggggcgaac gctttcatca gcgagcttcc
     1441 cgacgggtac gaaaccataa tcacggaagg cggatttcag ctttccggcg gtcaacagca
     1501 gcggatcgca atcgcgcggg ccgtcttgtc gaagcgctcc gtcctgctgt tcgatgaggc
     1561 gacttccgcc atagacttcg agaccgaagc cgcgattcaa cactctctgc gcgaggcggc
     1621 gggcgaccag acgatcgtac tcatagccca tcggctttcc actgtgcgga acgccgatat
     1681 catctacgtg atcgatgacg gccgcgtggt cgaagagggg cgtcaccacg atctgatccg
     1741 cgcgaacggg ctgtacgcaa gtctgtggcg aatccagacc ggcgaaacgg cgagccgttc
     1801 atagccgcca ggtcacgcag gcagtacctg gcctgccccg tttgcgcgcg cgcgctcaaa
     1861 gagcgacgcg cccaggacga ggtcggtcat tactgtcccc attgggttga agacgatccg
     1921 atcgctgccc gacaccgcga tctgcgcatc acccgccacc agcgcgccca atgttgttac
     1981 ccgcagatcg cgaagcctgc cgcgctgctg gagaacgtga agcggccgat tctcctgagt
     2041 acaaagttcg agatcgtcga ccacgacctg attcgcctgg gtaaaaacgt cgagctcggc
     2101 gtcaagcagg gaaactgccg cgtagagcga acccggcttc agccattcgc ccttgatcca
     2161 gggcgctccc gcggtggttg ccggtactac tatatcggcg cccgcgatcg cctcaccggc
     2221 tgcgctgcag acgtgggctg agacgccgag cgcggcgcaa tgcttcacga gggcctccgc
     2281 gcggctccgg tttatatcga agagggcgaa aacagcggca ggttcgggga atatctggct
     2341 ccacatcctg acatgtaccc aggcgagggc gcccgatccg ataagagcca ctttctcgac
     2401 gcgagggggg cgcaggaggg aaattgccag acccgtaatc gcggccgtgc gcaccgcgct
     2461 cacatgcgag gcgctgatca tgcagtaaat ccgctgcgtt tcggtgtcac agaggatgag
     2521 aatcccggaa gcgcgcggca ggttgcgccg cacgttcaac gtagaactgt tgatgagctt
     2581 tgctcccgca atctctcgtc cgttcccgac gtaacccagc agcgagatca tgcgatcgta
     2641 agcggatcgc ccgttgtatc ccaggtactc ggagcgcggc attctggtgt gtccggcatg
     2701 gtgaatccgc agcacatcga ccatgagagg cagcgcgcgt ttgaagtcat atacatcgag
     2761 atcgagcacg tcccgctcgg aaaggtagag aatggactgg ccactgataa gttcgctgac
     2821 gtagttggtt tggctcattc caattcctcc cggacggccg cccgttcaga ccgccgccgc
     2881 ggcggtcgcg ctgagtggcg cgccaaccca ttcgtcgtcg taaatcgtgt cgaggtattt
     2941 gtatccggaa tcggccgcga tgatgaccac cgtggagccg gat
//
LOCUS       DASZMC010000508         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1850787,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000508 DASZMC010000000
VERSION     DASZMC010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850787"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1325)
                     /locus_tag="VG274_10445"
     CDS             complement(81..1325)
                     /locus_tag="VG274_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE07119.1"
                     /translation="MTTYVHAGTLLARPGREPLTRHTIAIDGGRISAVAAGFTAPQSD
                     DVVIDLSDCFVLPGLIDCHVHLTGEFGPKYKLDLVEESPAAVALHAARHARVTLEAGF
                     TTVRDLGEIGAPGDAIFALRNAVAKGFVPGPRIFAAGSIISPTGGHGVTCGYRDDINF
                     LLDANGRGDGADGCRVAVRRQVSRGADFIKFVATGGVLTDTATGTGQQFFDDEYAAIV
                     ATARMLGRRTTAHAHGADGMKAALHAGVDGIEHGTFMDEETIDIMRRRGTFYVPTTLA
                     GVTVAEYATTQDFMPPAIRDKALAVGPQIVETLRRGHDAGLKIAFGTDTAVSPHGENA
                     REFALMVQAGMTPMEAIVAATVTAAEHIGQHERLGAIEPGKAADIIATAASPLDDVSE
                     LRKVQFVMRDGKPFKHARAKAV"
     gene            complement(1538..2314)
                     /locus_tag="VG274_10450"
     CDS             complement(1538..2314)
                     /locus_tag="VG274_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWE07120.1"
                     /translation="MSAPAFNAYGVWAIYSFEMARALRTIWQSLVTPVITTSLYFIVF
                     GAAIGSRMSHVGGVSYGAFIVPGLILLSLFTQSIFNGSFGIYLPRFVGTIYELLSAPV
                     SPLEIVLAYVGAAATKSVVLGLVILATASLFVHIHVLHPVWMIGFLVLTSVTFSLFGF
                     ILGIWSKNFEQLQIIPMLIVTPLTFLGGAFYSIDMLPPMWRGVAYSDPVVYLISGFRW
                     SFYGTADVRVAFSLGATLGFFFVCLGIVSWIFKTGYRLKN"
     gene            complement(2311..3333)
                     /locus_tag="VG274_10455"
     CDS             complement(2311..3333)
                     /locus_tag="VG274_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE07121.1"
                     /translation="MNRIDLVDGSRAARGATNPSETEAPRNAAPPIVTIRNLTKTYAT
                     GLEALKPINLEIRKGEIFALLGPNGAGKTTLINIVCGIVTPTSGEIIAGGHDIRRDYR
                     AARMKIGLVPQELTTDRFESVWTTISFSRGLFGRAPNDAYMEGVLRDLSLWEKRKDQI
                     QSLSGGMKRRVMIAKALSHEPSILFLDEPTAGVDVELRRDMWALVRRLRDGGVTIILT
                     THYIDEAEEMADRIGVINKGELILVEDKATLMKKLGQKQLTLVLQEPMSAIPEGLAEW
                     RLTLKAGGNELEYRFDINAEHTGIPSLLRRLSDLGIGFRDLATTQSSLEDIFVSLVSD
                     RGSNSR"
     gene            complement(3467..>3678)
                     /locus_tag="VG274_10460"
     CDS             complement(3467..>3678)
                     /locus_tag="VG274_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07122.1"
                     /translation="ALAEEPWATNSALDQAKITVACAVFHVRLVDPDTVPAGRFPVLD
                     RLSAQCEERPEFAATRPMTYTLPRG"
BASE COUNT          670 a         1177 c         1145 g          686 t
ORIGIN      
        1 cccgcgtcgt gggctacggg attcacgcat cgtgactgtc acaggttcct cctccgcgcg
       61 aagcgcgggg gaggaaccac tcacaccgcc tttgctctcg cgtgtttgaa tggttttccg
      121 tcgcgcatca cgaattgaac tttccgcagc tcgctcacat cgtcgagcgg gctggccgcg
      181 gtcgcgatga tatccgcggc cttgcccggt tcgatcgcgc cgagacgctc gtgctggccg
      241 atgtgctcgg ccgcagtgac ggtcgcggcc acgatcgcct ccatgggagt catgcccgcc
      301 tgcaccatca gggcgaattc gcgtgcgttc tcgccgtgcg gcgaaaccgc ggtgtcggtg
      361 ccgaaggcga ttttcagtcc cgcgtcgtgc ccgcgcctga gcgtctcgac gatctgcgga
      421 cccaccgcga gcgccttgtc gcggattgcg ggcggcatga agtcctgcgt cgttgcatat
      481 tccgcgacgg tgacgccggc gagcgtcgtc ggcacgtaaa acgtcccgcg ccggcgcatg
      541 atgtcgatcg tctcctcgtc catgaacgtg ccgtgctcga tgccgtccac cccggcgtgc
      601 agggcggcct tcatgccgtc ggcgccgtgc gcatgcgcgg tggtccttcg ccccagcatc
      661 cgcgcagtcg ccacgatcgc cgcatattcg tcgtcgaaga attgctggcc ggtgcccgtc
      721 gccgtgtccg tcaggacgcc gccggtggcg acaaatttga tgaaatcggc gccgcgcgac
      781 acctgccggc gcacggcgac acggcagcca tccgcgccgt ctccgcgccc gttcgcgtcg
      841 agcaggaaat tgatgtcgtc gcgatagccg caggtgacgc catgtccccc ggtcggcgaa
      901 atgatcgagc cggcggcgaa gatgcgcggg cccggtacaa atcccttcgc caccgcgttg
      961 cgcaacgcga agatggcgtc gccgggcgcg ccgatctctc ccagatcgcg caccgtcgtg
     1021 aatccggcct ccagcgtcac gcgcgcgtgg cgcgcggcat gcaacgccac cgctgccggc
     1081 gattcctcca ccagatccag tttgtatttc ggtccgaatt cgccggtgag gtggacatgg
     1141 caatcgatga ggcccggcag cacgaaacag tcggacaggt cgatcaccac atcgtcggat
     1201 tgcggcgcgg tgaagccggc ggcgactgcc gagatacgcc ccccgtcgat tgcaatcgta
     1261 tgcctggtga gcggctcccg tccgggcctc gccagcaggg ttccggcgtg aacgtaagtg
     1321 gtcatcgctc gcgacctttc ctttgcgtac gatagatttt taacgcagag gacgcggagg
     1381 gtgcgctgag gatgcagagg aatttcttgc gcgccacgtg gtcgggttaa acccgaccat
     1441 tccgcgcggt cgtcttcctg cgtccactgc gctgcctccg cgtcctctgc gttaaatctg
     1501 ccttcttgtc atccggcagg caaccgccac atcgtactca gttcttgagc cggtatcccg
     1561 tcttgaaaat ccagctcacg atgccgaggc aaacgaagaa aaaacccagc gtggctccca
     1621 gactgaaggc cacgcgcacg tcggcggtgc cgtagaaact ccagcggaag ccgctgatca
     1681 gatagacgac cgggtcggaa taggcgacgc ccctccacat cgggggcagc atgtcgatgg
     1741 aatagaaagc cccgcccaga aacgtcaggg gcgtaacgat cagcatggga atgatctgca
     1801 gctgttcgaa attcttcgac cagatgccga gtatgaatcc gaacaggctg aaggtgacgg
     1861 atgtcagcac caggaagccg atcatccata ccggatgcag aacgtgaatg tggacgaaca
     1921 gcgaagccgt tgccaggatc acgagaccca gcacgacgga tttggtggcc gcggcgccga
     1981 cataggcgag cacgatctcg agcggcgaca ccggcgccga caggagttcg tagatcgtgc
     2041 cgacgaatcg cggcaggtag attccgaacg aaccgttgaa gatgctctgg gtgaacagcg
     2101 acagcaggat caggcccggc acgatgaagg ccccgtagga aacgccgccg acatgggaca
     2161 tgcgcgagcc gatcgccgcc ccgaagacga tgaaatacag cgaggtcgta atgaccggcg
     2221 tgaccaggct ctgccagatg gtgcgcagcg cgcgcgccat ttcgaaactg tagatcgccc
     2281 acacgccata agcgttgaaa gccggcgcgc tcatcgcgaa ttgctcccgc ggtcgctgac
     2341 aaggctgacg aaaatatcct ccagcgaact ctgcgtcgtt gcgaggtccc tgaatccaat
     2401 tccgagatcg ctgagccgcc gcagaagaga aggaattccc gtatgctcgg cgttgatgtc
     2461 gaagcgatat tcaagctcgt tgccgccggc cttgagcgtc aggcgccatt cggcaagccc
     2521 ctcggggatc gccgacatcg gctcctggag aaccagggtc agctgcttct ggcccagctt
     2581 cttcatcagc gtcgccttgt cctcgaccag aatcagttcg cccttgttga tcacgccaat
     2641 gcggtcggcc atttcctcgg cctcgtcgat gtagtgcgtc gtcaggatga tggtgacgcc
     2701 accgtcgcgc aggcgccgca ccagcgccca catgtcgcgg cgcagctcga catccactcc
     2761 tgccgtcggt tcgtcgagaa agagaatgct gggctcgtgg ctcagtgcct tggcgatcat
     2821 cacccggcgc ttcatgccgc ccgacaggct ttgaatttgg tctttccgct tctcccacag
     2881 cgacagatcg cgcagcacgc cctccatata ggcgtcgttc ggcgcacggc cgaacaggcc
     2941 gcggctgaag ctgatcgtgg tccagaccga ttcgaaccgg tcggtggtca gctcctgcgg
     3001 caccagaccg attttcatgc gcgcggcgcg atagtcgcgc ctgatgtcat gaccgccggc
     3061 gatgatttca ccgctcgtcg gcgtgacgat tccgcagacg atgttgatca gcgtcgtctt
     3121 tccggcgccg ttggggccca gcagcgcaaa tatctcgccc tttcggattt ccaggttgat
     3181 cggcttcaag gcctcgagcc cggtggcgta ggtcttggta agattgcgga tcgtgacgat
     3241 cggcggggcc gcattgcgcg gggcctccgt ctccgacgga tttgtggcgc ccctcgccgc
     3301 cctggaaccg tcaacaagat cgattcgatt cacgcctgct ctcccgtcct tacagcccga
     3361 ctcgcagatg gtaacgcgca ccgtgtttcg caaaccacaa attgtggacg ttgattatcc
     3421 gaattgcggc ggctgtgcac agcggtgagc cggcatgcct gtgcttctat ccgcgcggga
     3481 gcgtgtaggt catcgggcgc gtcgcggcga attcgggccg ttcttcgcat tgagcggaaa
     3541 gcctgtcgag gacaggaaag cggccagctg gaacggtatc cggatctacc aggcgcacat
     3601 gaaagacggc gcatgcaacc gtgatctttg cctggtcgag cgcggaattc gtggcccagg
     3661 gttcctccgc aagagcag
//
LOCUS       DASZMC010000509         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2414488,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000509 DASZMC010000000
VERSION     DASZMC010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414488"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VG274_10465"
     CDS             <1..792
                     /locus_tag="VG274_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWE07123.1"
                     /translation="NNRDQPPAWPTSDDPRREEPSLDTLVPDNANKPYDMKELILKIA
                     DEGDFFEIQENFAKNMITGFGRLEGSTVGFVANQPMVLAGVLDSDASRKAARFVRFCD
                     CFNIPIVTFVDVPGFLPGVAQEHGGIIKHGAKLLFAFTEATSPKVTVITRKAYGGAYD
                     VMASKHMRADVNYAWPTAQIAVMGAKGAVEIIFRQDIGDKEKIAARTKEYEDRFLNPF
                     IAASRGYIDDVIRPHSTRWRVIRALRMLRKKQVPQIWKKHGNIPL"
     gene            complement(884..1558)
                     /locus_tag="VG274_10470"
     CDS             complement(884..1558)
                     /locus_tag="VG274_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWE07124.1"
                     /translation="MARLARVVAAGSPHLVTQRGNRGQKVFFSDADYELYKSLLAEGC
                     REAKVSVWAYCLLPSRVHLILVPRDEDGLWAALGEAHRRYTREINARKKWRGYLWQGR
                     FSSFPTDEPHLLACARYVELAPLRAKLVKRARDWRWSSAQAHLRSRDDGLVTARPLVD
                     RAGHWSEFLGEGMSAEEQEAIRSHESTGRPLGTPAFVARLEKRLGRTLARQKPGPKPK
                     KKRERK"
     gene            complement(1601..1984)
                     /locus_tag="VG274_10475"
     CDS             complement(1601..1984)
                     /locus_tag="VG274_10475"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWE07125.1"
                     /translation="MEFISSHGTKSIGLPFSDAVRTGDVLHLSGQIGNLPGRLELVPG
                     GLGPETRQMMENIARTLNSAGLSFNDVFKCTAMLADMSRWAEFNEIYVSYFESDRLPA
                     RSAFAVSALALGAAVEMECAAWMGT"
     gene            2077..4074
                     /locus_tag="VG274_10480"
     CDS             2077..4074
                     /locus_tag="VG274_10480"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HWE07126.1"
                     /translation="MFQKILIANRGEIACRIVKTVRRMGIATVAVYSEADAAALHVDM
                     ADEAVEIGAAPASQSYLNIERILQACRDTGADAVHPGYGFLSERVGFVEALAGSGIAF
                     IGPNAKAIAAMGDKIESKKLALAAGVSIVPGFVGEIRDAAHAKVLARDIGYPVMVKAS
                     AGGGGKGLRIVQREGELLQAIRSSQNEARASFGDDRLFVEKYVTEPRHIEIQVMGDKH
                     GHVIHLNERECSIQRRHQKVIEESPSPLLDAGTRKAMGEQAMALAKTVGYDSAGTVEF
                     IVDRDRNFYFLEMNTRLQVEHPVTELVTGFDLVELMIRSAAGEPLSIRQDEVRLDGWA
                     IESRIYAEDPNRGFLPSTGRLVRYRPPAEGGHGAATLRNDTGVCEGGEISIYYDPMIA
                     KLCSHAGSREAAIDAMDDALDAFRIEGISHNIDFLAAVMHHPRFREGRLSTGFIADEF
                     PDGFHGRPLDRDRARRFAAAAVAARILHGERARAISGTLNGPVPPLDAFRVSLGDREL
                     DVSEASMQGGIFRAIVDAGPYEAHVDWHPGCPVMRLAEGGADHALQMFRVRGGYRVSQ
                     GGSSAATVVYGPRAAELASLMPRKELADVSKLLLCPMPGLVVSVNVLEGQDVKLGETL
                     AVIEAMKMENVLTAEREGKIKKISARKGSSLALDDVIMEFA"
BASE COUNT          763 a         1216 c         1343 g          752 t
ORIGIN      
        1 aacaatcgcg accagcctcc cgcatggccg acaagcgacg atccgcgccg cgaggaacct
       61 tcgcttgata cgctcgtgcc ggacaacgcg aacaagccct acgacatgaa ggaactgatc
      121 ctaaagattg cggacgaggg cgattttttc gaaatccagg agaattttgc gaagaacatg
      181 attaccggct tcggccggct cgaaggatcg acggtgggct tcgtcgccaa ccagccgatg
      241 gtgctggcgg gcgtgctcga ttcggatgcg agccgcaagg ccgcgcgctt tgtccgtttt
      301 tgcgactgct tcaacatccc catcgtcacc ttcgtggacg tcccggggtt tcttcccggc
      361 gttgcgcagg agcatggcgg catcatcaag cacggcgcga agttgctctt tgccttcacc
      421 gaggcgacct cgcccaaggt cacagtcatt acccgcaaag cctatggcgg cgcttacgac
      481 gtgatggcgt ccaagcacat gcgcgccgac gtgaactatg cctggcccac ggcgcagatc
      541 gccgtgatgg gcgccaaagg cgcggtggag atcattttcc gccaggacat cggcgacaag
      601 gagaagatcg ccgcgcgcac caaagagtat gaggatcgtt ttctcaatcc gttcatcgcg
      661 gcaagccggg gctatatcga cgacgtgatc cgcccccatt ccacgcgctg gcgggtgatc
      721 cgcgcgctcc gcatgctgcg gaaaaaacag gtcccgcaga tctggaagaa gcacgggaac
      781 atcccgctgt gaggttggtc gatggggggc gcccgccgag agcttcctca tcctgagctt
      841 gtcgaaggat gagacccaac tcagccgtag atttttgtgt gtgctacttt ctttcgcgct
      901 tcttcttcgg ttttggccca ggcttctgcc gcgcgagggt gcggccaagg cgtttttcga
      961 gccgcgcgac gaaggccggc gttcccagtg gccgcccagt gctctcgtgg gagcggatcg
     1021 cctcttgttc ctccgcgctc atgccttcgc cgaggaattc gctccagtgg ccggcacggt
     1081 cgacgagcgg gcgggccgtc accaggccat cgtcgcggct gcgaagatgc gcctgcgcgc
     1141 tggaccagcg ccagtcgcgc gcccgtttga cgagcttcgc tcgcaggggc gccagttcga
     1201 catagcgggc gcaggcgagc agatggggtt cgtcagtcgg gaaggaagaa aatcgcccct
     1261 gccagagata gccgcgccac ttcttgcggg cgttgatctc gcgtgtgtag cggcgatgtg
     1321 cttcgccgag cgccgcccac agcccgtctt cgtcgcgtgg cacgaggatg agatgcacgc
     1381 ggctggggag caggcaataa gcccagacgg acaccttcgc ttcgcggcat ccctccgcaa
     1441 gcagcgattt gtacaattca tagtcggcgt cggaaaagaa tactttctgg ccgcggttgc
     1501 cccgctgcgt caccagatgg ggggacccgg ccgccaccac tctcgccaat cgtgccatca
     1561 ccgcaccttg gcgattgaga aggggtctgt caattccgta ctatgtcccc atccatgcgg
     1621 cgcattccat ctccacggcc gcgccgaggg cgagggcgga aactgcgaac gccgaacgcg
     1681 cgggcaggcg atcggattcg aaataggaaa catagatttc gttgaactcg gcccagcgcg
     1741 acatgtccgc cagcatcgcg gtgcatttga acacgtcgtt gaatgacagg cctgcggaat
     1801 tgagcgtgcg cgcgatgttc tccatcatct ggcgcgtctc agggcccagg ccgcccggca
     1861 caagttcgag acggccgggc agattgccga tctggccgga gagatggagc acgtccccgg
     1921 tgcggacggc gtccgagaac ggcaggccga tgcttttcgt gccgtgcgag gaaatgaatt
     1981 ccatgccgcc gcctccctgg gagcctgccg tttaccggcg gttcgcgagc gcctaaaacc
     2041 tagtgtcaca gtgtcgatgg cgggggaagg ggccgcgtgt tccagaaaat cctgattgcc
     2101 aatcgcggcg aaatcgcctg ccgcatcgtc aagacggtgc ggcgcatggg gatcgccacg
     2161 gttgcggtct attccgaggc cgacgcggct gcgcttcacg tggacatggc ggacgaggcg
     2221 gtcgagatcg gcgccgcgcc ggcctcgcaa tcctatctga acatcgaacg gattttgcaa
     2281 gcctgccgcg acacgggagc cgacgccgtt catccgggct atggatttct ctcggagcga
     2341 gtcggctttg tggaggctct cgccgggtcc ggcatcgcat tcatcgggcc gaatgcgaag
     2401 gccatcgccg cgatgggcga caaaatcgag tcgaagaagc tcgctctggc ggcgggtgtt
     2461 tcgatcgtgc caggcttcgt gggcgagatc cgcgacgccg cccacgccaa ggttctggcg
     2521 cgcgacatcg gctatccggt gatggtgaag gcttcggcgg gcggcggcgg caaggggctg
     2581 agaatcgtgc agcgggaggg cgaattgctg caggcgatcc gatcgtcgca gaacgaggcc
     2641 cgggcgagct tcggcgacga ccgcctgttc gtggagaaat acgtcactga gccgcgccac
     2701 atcgaaattc aggtgatggg cgacaagcac ggccatgtga tccacctcaa cgaacgcgag
     2761 tgctcgattc agcgccggca ccagaaagtg atcgaggaat cgccttcgcc tcttctcgac
     2821 gccggcacgc gcaaagcaat gggcgagcag gcaatggcac tcgcgaagac tgtcggctat
     2881 gacagcgccg gcacggtcga gttcatcgtc gatcgcgaca ggaatttcta tttcctcgaa
     2941 atgaatacgc gactgcaggt cgagcacccg gtgaccgaac tggtgaccgg cttcgatctg
     3001 gtcgaactga tgatccgctc cgctgcgggg gaacctcttt cgatacgcca ggacgaagtc
     3061 cggctggacg gttgggcaat cgagtcgcga atctatgccg aagaccccaa tcgcggtttc
     3121 ctgccttcga cgggaaggct tgtgcgctac aggccaccgg cggaaggcgg acacggagcg
     3181 gcgactctgc gcaatgatac cggcgtgtgc gaaggtggag agatttcgat ctattacgat
     3241 ccgatgatcg ccaagctgtg ttcgcacgcg ggatcgcgcg aggcggcaat cgacgcgatg
     3301 gacgatgcgc tcgatgcgtt tcgcatcgag gggatcagcc acaacatcga ttttcttgcc
     3361 gccgtcatgc accatccgcg tttccgcgaa ggcagacttt cgactggctt catcgcggac
     3421 gagttcccgg acggctttca tggccgtcct ctcgatcggg atcgcgccag gcgcttcgcc
     3481 gcggcggcgg ttgcggcgag gatacttcac ggcgaacgcg ctcgcgccat ttcgggcacg
     3541 ctcaacggac ccgtgccgcc gcttgatgcg ttccgggtat cgcttggaga ccgggaactg
     3601 gacgtgtccg aagccagcat gcagggggga atattccgcg cgatcgtaga tgcaggacca
     3661 tacgaggcgc atgtcgattg gcatcctggc tgcccggtca tgcggctggc cgagggcggc
     3721 gccgatcacg cacttcagat gttccgggtg cggggaggat atcgcgtgtc ccagggggga
     3781 tcgtcggctg caacggtggt gtacgggccg cgggcagctg agcttgcatc gttgatgccc
     3841 aggaaggaac tcgcggatgt gtcgaagctt ctgctttgcc cgatgccggg cctggtggtt
     3901 tccgtcaatg tcctggaggg ccaggacgtg aagctgggcg aaacgcttgc ggtcatcgaa
     3961 gcgatgaaaa tggaaaacgt gctgaccgcg gagcgggagg gcaagatcaa gaagatcagc
     4021 gccaggaagg gctcaagcct tgcgctcgat gacgtcatca tggagttcgc atga
//
LOCUS       DASZMC010000510         4906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5156582,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000510 DASZMC010000000
VERSION     DASZMC010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5156582"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2142
                     /locus_tag="VG274_10485"
     CDS             <1..2142
                     /locus_tag="VG274_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWE07127.1"
                     /translation="TMFWEVPGRRLNVLAGSAIADLAKVAGALASDAAIKGLVITSGK
                     TNGFCAGAALDEMEGNAVGAKSSAEATAARYNGVMQFHRALRGLETCGKPVAAAINGL
                     ALGGGLEVALACHYRIVADDPKIQFGLPEAKVGLLPGGGGTQRLPRLIGAMATLQLIL
                     KGDTADPQKALSLGMVHAIAPVGELVARAKAWIGTSPNSVQPWDRKDFKLPGGGPYSS
                     GGSQVFTMGNAMLRKETYGNYPAQRYIMSCVYEGIQLPIDAALRIEARYFVRLLMQPE
                     SRNMIRSLFLSLQELGKGARRPKDEPRTDVKTLGVLGAGVMGAGIAYVAALAGIEVVL
                     LDTTMEKANAGRDHAAGVIDRQIARGRSTPEKKAEILGRIHPANDFAALAAADLVIEA
                     VFEDRAVKAEATKKAEATIGANAIFGTNTSTLPITGLAEASARPTNFVGIHFFSPVDR
                     MGLVEIILGKRTAPHALAVAVDFVQKIRKTPIVVDDSRGFYTSRCFGTYTREGLAMLS
                     DGIHPAMIENVGRMAGMPMGPLEVMDSVGVDTALKITRETRKELGASGSDAAEQLLAW
                     LVEKSNRPGRKTGKGFYDYDAKGKRLRLWPDLVASRSGAWRTDADVEEMKARLLTIQA
                     LEAARCFEEQVITDPRDADVGAILGWGFAPFTGGPVSLIDTMGAAAFVARCETFAAKY
                     GDRFRPNRLLRDMAERNDTFYARFAPQKAAA"
     gene            2337..3203
                     /locus_tag="VG274_10490"
     CDS             2337..3203
                     /locus_tag="VG274_10490"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWE07128.1"
                     /translation="MNYEQISTTLRDNILTLTLNRPGKLNAFTRLMMTEMIDVFERAN
                     DNDEVRAIVVTGAGRAFCAGADLSAGAETFSRHNAELEIELSDERFRDGGGRLTLAIY
                     ECLKPVIAAVNGPAVGVGATMLLAMDIRIASDDARFGFVFARRGIVPEACSSWFLPRL
                     VGMQQALDWCYSGRVFDAQEALRGGLVKEMMKPEELQPRATALAAEIRDNAAPVSVAL
                     IRQMMWRLSALDHPMEAHKIDSRGIYARGKSADVREGVAAFLEKRPAKFPDKVSTDMP
                     AYFPWWSERRYS"
     gene            3604..3843
                     /locus_tag="VG274_10495"
     CDS             3604..3843
                     /locus_tag="VG274_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4424 family protein"
                     /protein_id="HWE07129.1"
                     /translation="MLSTGNSWNGPIGHFHLVLDKLEPGNVLSLCLDGALARKGPATF
                     ESTRERFAPEGDLGILVLQQSPRQRVRLPISAGVR"
     gene            complement(4125..4460)
                     /locus_tag="VG274_10500"
     CDS             complement(4125..4460)
                     /locus_tag="VG274_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07130.1"
                     /translation="MTSTCRNARRSFCLAALALVLAGCSIFGSSSDRAARNSPDYREG
                     YGDGCAAATDQGADLRDRPVGDASLYKSDETYRAGWSSGFQICRRTDMQPGASPADNP
                     IRDPVPGAH"
     gene            complement(4457..>4906)
                     /locus_tag="VG274_10505"
     CDS             complement(4457..>4906)
                     /locus_tag="VG274_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWE07131.1"
                     /translation="MTRSPPTIRDDASVADAAGALVAQRQMNLPVVDGEGRYAGMFGI
                     GDLLSLLVPRVALAGNLMANLRFVGDDPDELRRKFQEVKNLRVADVADRNAAAFDPES
                     SEIEAIRHFCRNPSALPVVEKESRKVVGMISSGDAIRAIAGSASSPK"
BASE COUNT          902 a         1528 c         1637 g          839 t
ORIGIN      
        1 acgatgttct gggaggtgcc gggacgcagg ctgaacgtgc ttgcgggcag cgcgattgcg
       61 gatctcgcga aagtcgcagg cgcgcttgcg tccgatgccg cgatcaaggg tttggtcatc
      121 acgagcggga agacgaacgg cttctgcgcc ggcgcggcgc tcgatgaaat ggagggaaac
      181 gccgttggcg caaaatcttc ggctgaggcg acggctgcgc gctacaatgg ggtgatgcag
      241 ttccaccgcg cgctgcgcgg gctcgaaacc tgcggcaagc ctgtcgctgc ggcgatcaac
      301 gggctggcgc tgggcggcgg actggaagtc gctctcgcct gccactatcg catcgtggcg
      361 gacgatccga aaatccagtt cggcctgccg gaagcgaaag tcgggctcct gccgggtggc
      421 ggcggcacgc aacgcctgcc gcggctgatt ggggcgatgg ctacgctcca gctcatcctg
      481 aaaggcgaca ccgccgatcc gcaaaaggcg ctgtcgctcg gaatggtgca tgcaattgcg
      541 cctgtgggcg aactcgtggc gcgcgcgaaa gcctggatcg ggacgtcgcc caacagcgtg
      601 cagccctggg accgcaagga ttttaaactg ccgggcggcg gaccctattc cagcggcggc
      661 agccaggtct tcacgatggg caatgcgatg ctgcgcaagg agacctacgg aaattatccg
      721 gcgcagcgtt acatcatgtc ctgcgtctat gaaggcattc agctacccat cgacgccgcg
      781 ttgcggatcg aggcgcgcta tttcgtcagg ctgctgatgc agcccgagag ccgcaacatg
      841 atccgctcgc tgttcctgtc gctgcaggaa ctcggcaagg gcgcgcggcg gccgaaggac
      901 gaaccccgca cggacgtcaa aacgctcggc gtgctcggcg cgggcgtcat gggcgccggc
      961 atcgcctacg tcgcggcgct tgcgggaatc gaggtggtgc tgctcgacac gacgatggag
     1021 aaggcgaatg ccggccgcga tcatgccgcg ggcgtgatcg accggcagat cgccaggggg
     1081 cgctcgacgc ccgaaaagaa agccgagata ctcgggcgca ttcatccggc caatgatttc
     1141 gcagcgctcg cggccgccga tctggtgatc gaagcggtgt tcgaggatcg cgcggtgaaa
     1201 gccgaagcaa cgaagaaagc ggaggccaca atcggcgcca acgcgatctt cggcaccaac
     1261 acgtcgacgc taccgatcac cggcctggcc gaagcaagcg cacgtccgac gaatttcgtc
     1321 ggcatccatt tcttctcgcc ggtggaccgc atgggcctgg tcgagatcat tcttggcaag
     1381 cggaccgcac cgcacgcgtt ggcagtggcg gtcgatttcg tgcagaaaat ccgcaagacg
     1441 ccgattgtcg tcgacgattc gcgcggcttc tatacgtcgc gctgcttcgg cacctacacg
     1501 cgcgaaggct tggcgatgct ctccgatggc attcatcccg cgatgatcga gaatgtcggg
     1561 cgcatggcgg gaatgccgat ggggccgctc gaagtcatgg attcggtggg cgtcgacacc
     1621 gcgctcaaga tcacgcgcga gacgcgcaag gagttgggcg cttcgggcag cgacgccgcc
     1681 gagcagctgc ttgcgtggct ggtggagaaa tcgaaccggc cgggccgcaa gacgggcaag
     1741 ggcttctacg actacgatgc gaaaggcaag cgtttgcggc tgtggccgga cctcgttgcc
     1801 tcgcggagcg gcgcctggcg caccgatgcg gacgtcgaag agatgaaggc gcgcttgttg
     1861 accatccagg cgctcgaagc cgcgcgctgc ttcgaggagc aggtgatcac cgatccgcgc
     1921 gatgccgatg tcggcgcgat cctcggctgg ggcttcgcgc ccttcaccgg tgggccggtc
     1981 tctttgatcg ataccatggg cgccgccgct ttcgtcgcgc gctgcgagac cttcgccgcg
     2041 aagtatggcg accggttccg tcccaaccgg ctcctgcgcg acatggcgga gagaaacgac
     2101 acattctacg cccgcttcgc cccgcagaaa gcggccgcgt gaacgagcga agaaaaggaa
     2161 gtatttaacg cagaggacgc tgaggttgcg caggggacgc agaaaaatga ttgcgcgcca
     2221 aaggcgcgtg attcttacct cagcgtcctc cgcgcaacct ctgcgtcctc tgcgctaaac
     2281 cctttttttt ccgtgattaa cttcttccgt tgtgcatatc gacaggaaca gggatcatga
     2341 actacgagca gatatcgacg acgctgaggg acaacatcct cacgctgacg ctcaatcggc
     2401 ccgggaagct caacgccttc acgcgcctca tgatgaccga aatgatcgat gtgttcgagc
     2461 gcgcgaacga caatgacgag gtgcgcgcca tcgtggtcac cggcgccggc cgcgccttct
     2521 gcgcgggggc cgacctgtcc gccggagcgg aaacgtttag tcggcataat gcagagctcg
     2581 aaattgaact tagcgatgag cgcttccgcg acggcggcgg ccggctgacg cttgcgatct
     2641 acgaatgcct gaagcccgtc attgctgcgg tgaacggccc ggcggtcggc gtgggcgcaa
     2701 cgatgctgct ggcgatggat atccgcatcg cgagcgacga cgcccgcttc ggcttcgtgt
     2761 tcgcgcgccg cggcattgtg cccgaagcct gctcgagctg gtttctgccg cggcttgtgg
     2821 gcatgcagca ggcgctcgac tggtgctatt ccggccgcgt gttcgatgcg caggaagcgc
     2881 tgcgcggcgg gctggtgaag gaaatgatga agcccgaaga gcttcagccg cgcgccactg
     2941 cgctcgccgc cgagatccgc gacaacgctg cgcccgtctc cgtcgcgctc atccgccaga
     3001 tgatgtggcg cctctccgcg ctcgatcacc cgatggaagc acacaagatc gacagccgcg
     3061 gcatctacgc ccgcggcaag tcagccgatg tgcgcgaagg cgtggccgcc ttcctcgaaa
     3121 agcgcccggc gaagtttccg gacaaggtct cgacggatat gccggcttac tttccgtggt
     3181 ggagcgagcg gagatattcg tagttacgca attctaattt cctcccccat cgtgggggag
     3241 gtggtttgcg cagcgaagcg aagcaaaccg gaggggggag tccgcgcggc gcgcgccact
     3301 tcccctcacc gcctcgcgga gtttatcctc cggccgcgct tcgcgcggag cggggggctc
     3361 gggacctccc ccggaggaaa accaagtgtc cggcgcgcga gtcaaattct gtgctgacga
     3421 cctcgctcgg attcgtaaga aaatttgtgc caacgccagt accgtgtagg agagggaaac
     3481 actcgatgtc gcccgggcgc cgatgcgcgg gtggggggta tccgcgtctt tatgcttggg
     3541 gcagattacc tggatcgggg ttgatagaac ggcggttatc tcaccgcgct gaccaccgac
     3601 tacgtgctat cgaccgggaa cagctggaac ggcccgatcg ggcatttcca tcttgtgctc
     3661 gacaagctcg agcccggaaa cgtcctctcg ctctgcttgg acggcgcgct cgcgcgcaag
     3721 ggtcccgcga cgttcgagag cacgcgcgaa cgcttcgcgc ccgaaggcga tctcggaatt
     3781 ctggtattgc agcagtcccc ccgccagcgg gtacggctgc cgatcagtgc cggtgtccga
     3841 tgagcatgtg aaaaacgcgc atcatccagc ccgagccgcg gcgcgcaaca ggagcgggct
     3901 ctgccgccct tccgtagcgt ccaaacacaa ggtgacccaa catcgacata ctccgatgcc
     3961 gcctcccgac gtacacggac gatacgcccg ttggcgcgcg gccacaacgg caatccgtcc
     4021 gcgcctatca ccatgtggcg agaagcgtta acggcgaatt aagaatgccg gatggggtcg
     4081 gccgttccat gaacagcgca tgaagcttgt gcgacaggca atgctcagtg cgcgccgggc
     4141 acgggatcgc gaatgggatt gtcggcaggc gaggcgccgg gttgcatgtc ggtgcgccgg
     4201 caaatctgaa agccgctgct ccatcccgcg cgataggttt cgtcgctctt gtagagcgac
     4261 gcatcgccga cgggacgatc gcgcaaatcg gcgccttggt ccgtcgcagc ggcgcatccg
     4321 tccccgtagc cttctcggta gtccggactg ttgcgggcgg cgcgatcgct ggagctgccg
     4381 aagatagagc acccggcgag gacaagcgcg agcgccgcga ggcagaagga gcggcgcgca
     4441 ttcctgcacg tcgatgtcat ttggggctgc tcgcggatcc ggcgatggcg cggatcgcgt
     4501 cgccgctcga aatcattccc accaccttgc gcgactcctt ctcgaccacg ggcagcgcgg
     4561 acggattgcg gcagaagtga cggatggctt cgatctcgga cgattcggga tcgaacgccg
     4621 cggcattgcg atcggccacg tcggccacgc gcaggttctt cacttcctgg aatttgcgcc
     4681 gcagctcgtc cggatcgtcg ccgacgaatc gcagattggc catgagattg ccggcgagcg
     4741 ccacgcgcgg cacgagcagg ctgaggagat cgccgatgcc gaacatgccg gcatagcggc
     4801 cctcgccgtc gaccacagga agattcatct ggcgttgcgc aacaagcgcg cccgcagcat
     4861 ccgccacgct cgcgtcgtcg cggatggtcg gcggcgatct ggtcat
//
LOCUS       DASZMC010000511         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3515535,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000511 DASZMC010000000
VERSION     DASZMC010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3515535"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VG274_10510"
     CDS             complement(<1..616)
                     /locus_tag="VG274_10510"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HWE07132.1"
                     /translation="MDSFEPIRKAAEDLHHQVAGDGTPKKPMDLVNAAIKHLKLELTW
                     LPTGDPALKGARAVFDDQSGTIFAEDVGNESQRALVVAHEIGHECIHAGSIACSAEDV
                     DPSRSMEAAPVGLQRVEDYGAHERRELQANVFARAFLFPRSLARQMFVDQALTASDIA
                     KQLDLPVPLVRQQILDVILLPPPPMDEDSDSPLTRPSRPDPAQDR"
     gene            complement(620..1276)
                     /locus_tag="VG274_10515"
     CDS             complement(620..1276)
                     /locus_tag="VG274_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07133.1"
                     /translation="MITAATQTSLRDAMYAMSLAKAVPDAELLDEFARRYPQHADALT
                     EFAIELAIDSLMHQSDEEDVPTDADAISDVVSRVMSQFENRLYERRQARAATSPARAA
                     TASVENPFATLDRPGFRALASRLDINSAFLSKLRDRTIEPISIPKTYCRHLAEEMDEE
                     TEAMAAHLYAPQESVAAGRQLYKAEGKPAATARQSFEEAVKTSGLSEEQQRRLLSFRD
                     "
     gene            complement(1273..1812)
                     /locus_tag="VG274_10520"
     CDS             complement(1273..1812)
                     /locus_tag="VG274_10520"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWE07134.1"
                     /translation="MPQLLKRAEANLERTVPDSRMRDAKSVQHEILSDLAMMFTQDGT
                     EGHEDELDFYECKFLRALRFLRIDHVRKALSERKELTDLPEADDGEGEAAFDEEMLAR
                     LSRMASVGPSQEDRLYLPEVIKALDKLPPDQKRAVVLRRIIGHTEEQTAKICKVDKRT
                     IRYRLAAADKQLKTLKEDL"
     gene            1864..1995
                     /locus_tag="VG274_10525"
     CDS             1864..1995
                     /locus_tag="VG274_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07135.1"
                     /translation="MNETFRRQEIAGLRIRLARAPGEGGPILGQRDVDLPFDTRRAG"
     gene            complement(2220..3035)
                     /locus_tag="VG274_10530"
     CDS             complement(2220..3035)
                     /locus_tag="VG274_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07136.1"
                     /translation="MRRLGAELDRLTIVLTQDGDGLSVAELGAECSGIDLDAIAAKDQ
                     TVAEEVQELRNRLMEAREARNTARQTFEAIGGDDLAARDAADRQAALAEMTEIAEQYV
                     RLRSAVILLQWAIDRYRREKQAPMLKRAGQLFAILTCGSFQTLQLEFDEHDNMQLAGL
                     RHDGRRVPVGGMSTGTADQLYLALRIAAIEDYLDHAEPMPFIADDLFINFDDKRAAAG
                     FRVLGELAKKTQVLFFTHHEHLLEVARKALGTPITGVTLPVAASPPPPRSQAA"
BASE COUNT          557 a          968 c          986 g          652 t
ORIGIN      
        1 cgcgatcttg cgccgggtcc ggccgagacg gacgggtcag ggggctgtcg gaatcttcat
       61 ccatgggggg aggcggcaga agaatcacat ctaggatttg ctggcgcacg agcggcaccg
      121 gcaaatcgag ctgcttggcg atatcggacg ccgtcagggc ttggtcgaca aacatctgtc
      181 gcgcgagcga tctaggaaaa agaaacgccc gcgcaaacac atttgcttgc agctcccgac
      241 gctcatgcgc gccgtagtcc tcgacgcgct gcagacctac cggcgcggct tccattgaac
      301 gcgatggatc cacgtcctca gcgctgcagg cgatggatcc cgcgtggatg cattcgtgcc
      361 cgatctcgtg cgcaacgacg agcgcgcgtt gggattcatt gccgacgtcc tcggcgaata
      421 ttgtgccgct ttgatcgtcg aagacggcgc gcgcgccctt gagcgccgga tcgcctgtcg
      481 gaagccatgt caattcaagc ttgagatgct tgattgcggc attgaccaga tccataggct
      541 tcttgggcgt cccgtcgccc gccacctgat ggtgcaggtc ctccgcagcc ttccggatgg
      601 gttcgaacga atccattctc tagtcccgga aagagagcaa acggcgttgc tgctcttcgc
      661 ttaatccaga tgtctttacc gcttcctcga aactttgcct tgcggttgca gcaggctttc
      721 cctccgcctt gtagagttgg cggccggccg cgacggactc ttgtggcgca tagaggtgcg
      781 cagccattgc ttcggtttct tcatccatct cttcagcgag atgccggcaa tatgtttttg
      841 gaatcgaaat aggctctatc gtcctatccc gcagcttcga aaggaaggcg ctattgatgt
      901 cgagccgtga tgcgagcgca cgaaatcccg gccgatcgag cgtcgcgaat ggattttcca
      961 cggaagccgt cgcggcgcgg gcgggcgagg tcgccgcccg cgcctggcgg cgttcgtaga
     1021 gccggttttc aaactggctc atgacgcgcg acaccacgtc gctgatcgcg tcggcatcgg
     1081 tggggacatc ctcctcgtcg ctctggtgca tgagcgagtc aatcgcaagt tcaatagcga
     1141 attcggtgag cgcgtcggca tgctgcggat agcgccgcgc gaattcgtcg agcagttcgg
     1201 cgtccggaac agccttcgcc agcgacatgg cgtacatggc gtctcgcagg gacgtttggg
     1261 tggcggcggt gatcataggt cctccttcaa tgtcttgagc tgcttgtcgg cggcggcaag
     1321 gcggtaccga atcgtccgct tgtccacctt gcaaatcttt gcggtctgtt cttccgtgtg
     1381 tccgatgata cggcggagca cgacggcgcg cttttgatcc ggcggcagct tgtcgagcgc
     1441 cttgatcacc tcgggcaggt acagccggtc ttcctgcgac ggaccgacgc tcgccatccg
     1501 ggacagccgg gccagcattt cctcatcgaa agcggcctcg ccctcgccgt cgtcggcttc
     1561 gggcagatca gtcagctcct tgcgttcgga cagcgccttg cggacgtgat cgatccgaag
     1621 gaagcgcaac gcccgtagaa acttgcattc ataaaagtcc agctcgtcct cgtgcccttc
     1681 cgtgccgtct tgcgtgaaca tcatcgcgag gtcgctgaga atctcgtgct ggaccgactt
     1741 ggcgtccctc atccggctgt ccggcaccgt gcgctcgaga ttggcttcgg cgcgcttgag
     1801 gagctgcggc atcagcaccc tcgcgatacg gtcctcgccg cggcggaggg catcgcgaag
     1861 gagatgaacg agacattccg acggcaggaa atcgccggat tgcggattcg tctcgcgcgc
     1921 gcgccgggag agggtggccc aatcctgggg cagcgcgatg tcgatcttcc gttcgatacc
     1981 cggcgggcgg gttaggtcct tcttcgacaa cggacgtgcc atctcgcccc ctttcaagca
     2041 gcgggtttta ccgggcgtcc aattcgttcg gcccaccctt cactaggtga tcgaaaaaaa
     2101 gagggggtac ccggaaagct gaatccgcaa atcttttaag tctaatttca tcaagaaaaa
     2161 tcaacatgtt agatggagtg ataacacaag ttacggaaat agccgaagat tcggggccgt
     2221 tacgcagcct gcgaacgtgg cggcggcggg cttgcggcca ccggcagcgt aacgccggtg
     2281 atcggagtgc ccagggcttt tcgcgcgacc tccagcaggt gctcgtggtg cgtgaagaac
     2341 aagacttgcg tctttttggc gagctcgccc aagacacgga agcctgccgc cgcgcgcttg
     2401 tcgtcgaagt tgatgaacag gtcgtccgcg atgaacggca tgggctcggc atggtcgaga
     2461 tagtcctcga tcgcggcgat ccggagcgcg aggtagagct ggtccgcagt gccggtgctc
     2521 atgccgccaa ccgggacccg tcggccgtca tggcgtaggc cggcgagctg catgttatcg
     2581 tgttcgtcga attcaagctg cagcgtttgg aaagacccgc aggtgaggat ggcgaacagc
     2641 tggcccgcgc gcttgagcat cggcgcctgc ttctcccgcc ggtagcgatc aatcgcccat
     2701 tgcagcaata tgaccgcgga gcgaaggcgc acatactgct cggcgatctc ggtcatctcg
     2761 gcgagcgcgg cttgccgatc cgcggcgtcc ctcgccgcca ggtcgtcccc gccgatcgcc
     2821 tcaaaggtct ggcgggcggt attgcgggcc tcgcgcgctt ccatcagccg attgcgcaat
     2881 tcctgcactt cttcggcgac cgtctggtcc ttggccgcga tggcgtccag gtcgatgccc
     2941 gagcactcgg cacccaactc cgcaaccgaa agaccatcgc catcctgcgt cagcacgatc
     3001 gtaagccgat caagctcggc gccgagccgc cgcatctcgt cggagcgctg aattgcgatc
     3061 cgcaattcgt ctgtcgatgc gacattggcc ttctgctgga gctgtgcaat ggtttcgcgc
     3121 gcttcgcggc tcgattcacg gcagtcgtcg atcttctcct gca
//
LOCUS       DASZMC010000512         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1852850,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000512 DASZMC010000000
VERSION     DASZMC010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852850"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /gene="recN"
                     /locus_tag="VG274_10535"
     CDS             <1..1599
                     /gene="recN"
                     /locus_tag="VG274_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWE07137.1"
                     /translation="LLFGARAAAEMVRTGAERARISGIFEIDPTLLPEGVEAENGELI
                     IEREILANGKSRAFAGGRPVTAAVLREIAERLGDIHGQHDQQQLFSPATQLEMLDAFP
                     GSGSGAADLVTGVREAFARWHAVSDELAELERTSQEKLRQADLWKFQVQEIEAVMPES
                     GEDARLENERRVLRNVVRLEESAAAAYAALYEDPASVSVQLRAVVKRLEEVARIDESV
                     GEVIGALQPASIGIDEASHALRNYLGKLESDPARLEEIETRLAALEKLKRKYGATTLG
                     SAVGSTLEDVIAFLENARAQLAAVETSGERQESLRKELRALTAAYESAAGSLSARRKE
                     AAKSLSKRVQTELAALAMERSRVEIRVSPTEWSPSGIDAVEFLISPNVGEEPKPLEKI
                     ASGGELSRVALALKTCTAGSSRKTAIPRTAIPQTAIPQTLVFDEIDAGVGGSAAEAVG
                     RKLKKLAANSQVLCVTHLAQIAGFGDHHYFVEKHAERGRTVTTIEELSPAARTREIGR
                     MLSGERVTPEALRHAEQLMKLGAE"
BASE COUNT          325 a          497 c          525 g          266 t
ORIGIN      
        1 ctcctgtttg gagcccgcgc cgccgccgag atggtccgca ccggagccga acgcgcgcgc
       61 atttccggaa tcttcgaaat cgatcccacg cttcttcctg aaggtgtcga agccgagaac
      121 ggcgagctga taatcgaacg cgaaattctg gcgaacggca aatcgcgagc cttcgccgga
      181 ggccgcccgg tgacggccgc tgtgctgcgc gagattgccg agcgcctggg cgacattcac
      241 ggccagcacg atcagcaaca gctttttagt cctgcaacgc agcttgaaat gctcgacgca
      301 tttcccggtt ccggctccgg cgccgccgac ttagtcaccg gcgtgcgcga ggctttcgcc
      361 cgttggcacg cggtctccga cgaactggcc gaactcgaac gcacctcgca ggaaaagctg
      421 cggcaggccg atctgtggaa gtttcaggta caagagatcg aggcggtgat gcccgaatcg
      481 ggcgaggacg cgcgattaga gaacgagcgc cgcgtgctgc gcaatgtcgt gcgcctggaa
      541 gaatcggcgg cggcggccta tgccgcgctc tatgaagatc ccgcgagtgt ctccgtgcaa
      601 cttcgagccg tcgttaagcg cctggaagag gtcgcgcgga ttgacgaatc cgtgggcgaa
      661 gtgattggcg cgctgcagcc cgcctccatc ggtatcgacg aagcgtcgca cgcgctgcgc
      721 aattatcttg gaaagctgga atcggacccc gcgcgtctgg aagaaatcga gacaaggctc
      781 gcggcgcttg aaaaactgaa gcgaaagtac ggtgcaacga ccttgggctc agcagtgggt
      841 tcaacattgg aagacgtcat cgcgttcctg gaaaacgccc gcgcacagct cgccgcggtg
      901 gaaacttcgg gcgagcgcca ggaatccctg cgcaaggaat tacgcgcgct gactgccgcg
      961 tacgaatccg ctgccggatc gctttccgcg cgtcgcaagg aagccgcgaa aagtctgtcg
     1021 aagcgcgtac agacggaatt ggccgcgctg gcgatggagc gttcccgcgt ggagatccgc
     1081 gtgtcgccca ctgaatggtc gccgtcggga atcgatgccg tggagttcct gatttcaccc
     1141 aacgtgggcg aggagccgaa gcctctcgaa aaaatcgcct caggcggcga gctctctcgc
     1201 gtcgcgctgg cgctcaaaac ctgcacggcc gggtcttcgc gaaaaaccgc gattccccgg
     1261 acggccattc ctcaaacagc cattcctcaa acattagtgt tcgacgaaat cgacgcaggc
     1321 gtcggcggca gcgcggcgga ggccgtgggc cgcaaactca agaagttggc cgccaacagc
     1381 caggtgctgt gcgttacgca cctggcgcag atcgcgggct tcggcgatca tcattacttc
     1441 gtggaaaagc acgccgagcg cggccgcact gtgacgacga tcgaagagct gagcccggcg
     1501 gcgcgcacgc gcgagatcgg ccgcatgctt tcgggcgagc gcgtcacgcc tgaagcgctg
     1561 cgccacgccg agcaactgat gaagctcggg gcagagtagc ggttgacacg cgg
//
LOCUS       DASZMC010000513         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2960675,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000513 DASZMC010000000
VERSION     DASZMC010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2960675"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..973
                     /locus_tag="VG274_10540"
     CDS             71..973
                     /locus_tag="VG274_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE07138.1"
                     /translation="MFEGFTRTEIETPSSVFGGARIHLRHGGDGPPLLLLHGNPLTHV
                     SWHKIANRLAEHFHVVAPDLRGYGDSVGPDDAGPNNINYSFRAMAQDQVEVMEKLGYR
                     EFFVAGHDRGARTTARMCLDHPDRVKRAALVDILPNHHIWTHTSKEWELRSWHWALMA
                     QPCDLPERLLAGVSAQWFMEKKLSKPGIGLGFFDPEAFAEYVRCFDWKTIKGSCADYR
                     ACVTCDLDMDTADFAAGHKITVPLLVVWGQRSHTEGVHGDVLAVWQKNYATNAWGGAL
                     PCGHYVPEEAPDETYAKFIEHFRR"
     gene            999..1532
                     /locus_tag="VG274_10545"
     CDS             999..1532
                     /locus_tag="VG274_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07139.1"
                     /translation="MMQSPRQQSARELYWRQLAQLKADNVYIRLYRDDRSGWVSRLGI
                     LRAVASSGSIAAWAIWREYAFVWALVIAASQVADVLKDVFPFARQYQAAGEYMLTLDR
                     LLIDAEMEWSKVFRGDYPEDDILDRRRVLMTLQLEAQRKSFPSGLPPRNQGLWEVAEG
                     EAAAYLSKMYSTGERDD"
BASE COUNT          302 a          481 c          482 g          296 t
ORIGIN      
        1 tcgattagcg gtcgcgtgcg gcccagcctg cactaagttg cgggtcggcc aaggcgcagg
       61 aggcaggcaa atgttcgaag gctttactcg caccgagatc gagaccccct cctccgtctt
      121 tggcggcgcg cgcatccatc tgcgccatgg cggcgacggc ccgccattgt tgctgctgca
      181 cggcaacccg ctgacgcatg tctcctggca caagatcgcc aaccgcctcg ccgagcattt
      241 ccatgtcgtc gcccccgatc tgcgcggcta cggcgacagc gtcggcccgg acgatgccgg
      301 cccgaacaac atcaactatt cgttccgggc gatggcgcag gaccaggtcg aggtgatgga
      361 aaagctcggc taccgcgagt tcttcgtcgc cgggcacgac cgcggcgccc gcaccaccgc
      421 gcgcatgtgc ctcgaccacc ccgaccgggt gaagcgcgcc gcgctcgtgg acatcctgcc
      481 gaaccaccac atctggaccc acacctcgaa ggagtgggag ttgcgctcgt ggcattgggc
      541 gttgatggcg cagccctgcg atctgcccga gcggctgttg gccggcgtct cggcgcagtg
      601 gttcatggag aagaaattat cgaagccggg catcggcctc ggctttttcg atcccgaggc
      661 gttcgccgaa tacgtccgct gcttcgactg gaagacgatc aagggctcct gcgccgatta
      721 ccgcgcctgc gtcacctgcg acctcgacat ggacaccgcc gatttcgcgg ccggccacaa
      781 gatcaccgtg ccgcttttgg tggtctgggg ccagcgcagc cacaccgaag gcgtccacgg
      841 cgacgtgctc gccgtctggc aaaagaacta cgccaccaac gcctggggcg gcgccctccc
      901 ctgcggccac tacgtccccg aagaagcccc cgacgagacc tacgccaaat tcatcgagca
      961 tttcaggcgt tgatcgaaag gacttcgagg aacgaacctt gatgcagagc ccaagacagc
     1021 aatccgcccg ggaactttat tggcgacagc tggcacagtt aaaagctgat aatgtttaca
     1081 ttcgcttata tcgcgacgat cgctccggtt gggtttcgcg gctggggatc ttgagggctg
     1141 ttgcctccag tggaagcatt gccgcttggg cgatctggcg ggaatacgcg tttgtatggg
     1201 ctctggtgat agcagcgtcg caggtggctg atgtacttaa ggacgtattc ccgttcgcgc
     1261 gacaatacca ggcggcggga gagtacatgc tcacgctcga ccggctgtta atagatgctg
     1321 aaatggaatg gtcaaaggta ttccgaggcg attatccgga ggacgatatc cttgaccggc
     1381 gtcgtgtgtt gatgaccttg caactcgaag cgcaacgcaa aagcttccct agcggattac
     1441 cgccacgaaa ccagggctta tgggaggtag cggaaggcga ggccgcggct tatctttcga
     1501 agatgtattc tacgggtgaa cgcgatgatt gaggtgccga tgcctcgcgg caatccgact
     1561 g
//
LOCUS       DASZMC010000514         5403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1854679,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000514 DASZMC010000000
VERSION     DASZMC010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854679"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VG274_10550"
     CDS             complement(<1..409)
                     /locus_tag="VG274_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07140.1"
                     /translation="MRDFTVLGIETSCDETAAAVVRGRAPGPGEILSNVVFSQIEEHR
                     PYGGVVPEIAARAHVELLDQVIARALSQSKLALSQVDVVAATGGPGLIGGVMVGVTTA
                     KALALAAEKPFVAVNHLEAHALTARLTGGVAFPF"
     gene            489..1430
                     /gene="hemC"
                     /locus_tag="VG274_10555"
     CDS             489..1430
                     /gene="hemC"
                     /locus_tag="VG274_10555"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWE07141.1"
                     /translation="MTLQSPSSSTLRLGTRGSKLARAQADIVREALAARCGFAAELVA
                     IKTSGDRMRDRPLAEAGGKGLFAKELEEALLAEHIELAVHSLKDLPTILPDGLAIAAI
                     LPREDTRDAFISANARQLHDLPKGARLGTSSVRRAAQIARRRPDICVVPLRGNVDTRL
                     GKLDAGEADAIILAYAGLKRLGLQARATSLLPTEIWLPALSQGAMGIEMREQDPRRST
                     IAAALDDEMTSIAIACERAFQAALDGSCRTPIAGLATVADNRLVFRGEVLAPDGSACV
                     DTSLDLALGNNPLAEAAQAGREAGLAIRDRAQLWLAL"
     gene            1437..2162
                     /locus_tag="VG274_10560"
     CDS             1437..2162
                     /locus_tag="VG274_10560"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWE07142.1"
                     /translation="MRVLVTRPAEDASRTARMLARLGHDALIAPLLELRFLDGPEISL
                     AGVQAILATSGNGIRALARRSPRRDIPVFAVGTQTADLARAESYFEIVDAAGDSRALA
                     ALVRGRLRPDAGVLLHAAGTDSRDEWREQLRSAGFEVRRSVLYQALEAPELPAAAQTA
                     LRSGSIDALIVFSPRSAQIFADCVQRAGLASTCAHVTACCISDTAAGALKNIPFAAIH
                     VAAHPDHDAVLALLDNCARRGGL"
     gene            2189..3139
                     /locus_tag="VG274_10565"
     CDS             2189..3139
                     /locus_tag="VG274_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE07143.1"
                     /translation="MRKFLLGLVVFVAAIAGPLYYFSSPDIPRSALVAKYATPPSQFV
                     TLRDGARVHVRDRGPRTAPALVLIHGSNASLYTWEPWARRLDDAFRVVTLDMQGHGLT
                     GAVPSRDYSEPAMVRTVAEVAGRLGLARFAIGGNSMGGAVAARFVEEYPGRVTQLILV
                     DAGGMPTKEGDRIPLAFRLARIPVLNQILLHVTPRELVIEGLDDAIVRKSVIDDDMID
                     RYWDFARMEGTREATLERFNLPRDDYVSLHAGKIAVPTLVLWGEEDHLVPVEAGRMWA
                     RTIRSARLIVYPATGHLPQEEMADKSAADVRTFLLATSRP"
     gene            complement(3167..4453)
                     /locus_tag="VG274_10570"
     CDS             complement(3167..4453)
                     /locus_tag="VG274_10570"
                     /inference="COORDINATES: protein motif:HMM:NF037860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07144.1"
                     /translation="MHRSVQGAASHVMQDHASQAGQKALLIISGGRQAADAARRAKEL
                     GFYVIVADRDPEAPAFAVTDSILIADADGADETSAAAERFSRKIRRIDGAISMAANAP
                     MTMAAVARRLGLPGPSERISQLACDKLAMKRTLREAGLPVPWFAPVETPQALARIVIE
                     HGRDLVIKPVDSRESRGVQRLAAVADIDRAFALARSHSITGRVMVERCLDGPQIFAQS
                     LVLDGHCMTPVLFDRNFEYLERYAPFFVQNGGELPSALPAEIQKDTRALVAQAAQVLG
                     VTSGAVKAGIVVHDDKAQLIDLATTICGGALCTRAIPLSSGVDFIGAAMRLAVGDAVA
                     MEELEPLKTASVVQRYLFPQPGRVVSISGVEAACEIPGIADVIVTVRPGDVFPRSNDG
                     RPSAMMVLATGDARDIALSAANEALARIRIVTEAAG"
     gene            4946..>5403
                     /locus_tag="VG274_10575"
     CDS             4946..>5403
                     /locus_tag="VG274_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07145.1"
                     /translation="MARIFWFAVALAGASAAYAISGARDLVYDGSFYLLGIASHGAFQ
                     LFEPGRVSVQLLQQGFAVIGARLGIHDLWTLGRLFSLGASGWPIVLTALCWFVLPRAE
                     KSWIAGPLLNLVFAIPATNFIGIGEGVIASCLLWLAFLLAWFRMDRSWGAL"
BASE COUNT          922 a         1767 c         1707 g         1007 t
ORIGIN      
        1 gaaacggaaa cgcgacaccc ccagtcagcc gtgccgtcaa cgcatgcgct tccaggtgat
       61 tgacggcgac gaaaggtttt tccgccgcga gagccagcgc cttggccgtt gtcactccga
      121 ccatcacgcc gccgatgagt ccggggccgc cggtcgccgc caccacatcg acctgcgaaa
      181 gcgccagctt cgattgtgaa agcgcccgtg caatcacctg gtcgagcaat tcaacgtggg
      241 cgcgcgctgc gatctcgggc accacgccgc catagggacg atgctcttcg atctggctga
      301 agacgacgtt cgacaggatc tcgccggggc cgggcgcacg cccgcgcacc accgctgccg
      361 ccgtttcgtc gcagcttgtt tcgatgccga ggacagtgaa atcgcgcata tctgtgctgg
      421 tgtctcgtgt atcgctcgaa tccgattccg ggtagtagct tacagtgcgc cgcccgtaac
      481 caaagccaat gacgctgcaa agtccttcat cgtcgacact gcgcctcgga acgcgcggct
      541 cgaagctcgc tcgtgcgcaa gccgacatcg tacgcgaggc gcttgcggcg cgctgtggtt
      601 tcgcggccga gcttgtcgcc atcaaaacct ccggcgaccg gatgcgggat cgcccgctcg
      661 cggaggcggg cggcaagggg ctcttcgcca aggaactcga agaagcgctg ctggcggagc
      721 atatcgagct ggccgtgcat tcgctcaagg atcttcctac gatcctgccc gacggcctcg
      781 caattgcggc aatccttcca cgggaagata cccgcgacgc cttcatctcc gccaacgcca
      841 ggcagcttca cgatctgccg aaaggagcgc ggctgggaac gagttcggtg cgccgcgccg
      901 cccagattgc acgccggcgc cccgatattt gcgtggtccc gctgcgggga aacgtcgata
      961 cgcggctcgg caagcttgac gctggcgaag cggacgcgat catcctggcg tatgcagggt
     1021 tgaaacgcct cgggcttcag gcgcgcgcga catcgctcct gcccaccgag atctggttgc
     1081 ccgcactgtc gcagggcgcg atggggatag aaatgcgcga gcaggatccg cgccgctcga
     1141 cgattgccgc ggccctcgac gatgagatga cctcgatcgc aattgcctgc gagcgggcgt
     1201 tccaggcggc gctcgacggc tcatgccgca cgccgattgc gggccttgcc accgtcgcgg
     1261 acaatcggct cgtgttccgc ggcgaggtgc tggcgccgga cggaagcgct tgcgttgata
     1321 catcgctgga cctggcgctc ggcaacaacc cgcttgcgga agccgcgcaa gccggccgcg
     1381 aggcgggcct ggcaatcaga gaccgcgcgc aactgtggct cgccttgtga acggcgatgc
     1441 gtgtgctcgt caccaggccc gcggaagatg cgtcacgcac cgccaggatg ctcgccaggc
     1501 ttggacatga cgcgctgatc gcgccgctgc ttgaactgcg ctttctcgat ggtcccgaga
     1561 tttcgcttgc cggcgtgcag gccatactgg cgacgagcgg caacggcatc cgcgcactgg
     1621 cccgacgcag tccgcgacga gacattcccg ttttcgccgt cgggacgcag accgcggacc
     1681 tcgcgcgagc cgagagctat ttcgaaatcg tggatgcagc cggcgattcc cgagcgctcg
     1741 cagccctggt gcgcggtcgc ttgcggcctg atgccggtgt cctgctgcac gcggccggaa
     1801 cagactcgcg ggatgagtgg cgtgaacaac tccggagcgc cgggttcgag gttcgccgct
     1861 ccgtgcttta ccaggcactc gaagctccgg aactccctgc cgctgcgcaa accgcgttgc
     1921 gatcgggatc gatcgatgca ctgatcgtgt tctcgccgcg cagcgcgcaa atctttgcgg
     1981 actgcgtcca gcgcgcgggc ctggcttcca cgtgcgcaca cgtgaccgcc tgctgcatta
     2041 gcgatacggc ggcgggtgcg ctgaagaaca tccccttcgc cgcgatccat gtcgccgcac
     2101 atcccgatca cgacgccgtg ctcgcgcttc tcgataattg cgcgaggcgc ggcggccttt
     2161 agaatcgtga tctgccggga gggttcgaat gcgaaaattt ctgctgggcc tcgtcgtttt
     2221 tgtcgccgcg atcgccggcc ctctctatta tttcagctcg ccggacattc cccgcagcgc
     2281 gctcgtcgcg aaatacgcca cgcctccgtc acaattcgtc acgctgcgag acggcgcgcg
     2341 cgtgcatgtt cgcgacaggg gaccgcgcac agcacctgca ctggtgctca ttcacggctc
     2401 caacgcgtcg ctctacacct gggagccatg ggccaggcgt ctcgacgatg cgtttcgcgt
     2461 cgtcaccctg gacatgcagg gccacgggct gacaggcgcg gtcccgtccc gcgactattc
     2521 cgaaccggcg atggtcagaa ccgtcgccga agtcgccggc aggcttggcc tcgcccgctt
     2581 cgccatcggc gggaattcga tgggcggagc ggtcgcagcg cgtttcgtgg aggaatatcc
     2641 cgggcgcgtc acgcaattga ttctcgtcga tgcggggggg atgccgacga aagagggcga
     2701 caggattcca cttgccttcc ggcttgcgcg cataccggtg ctcaaccaga tcctgcttca
     2761 cgtcaccccg agagagcttg tcatcgaggg acttgacgac gccatcgtcc gcaaatccgt
     2821 catcgacgac gatatgattg accgctattg ggatttcgcg cgcatggaag gtacgcgtga
     2881 ggcgacgttg gagcgtttca atcttccccg tgacgactat gtgtcgctgc atgccggcaa
     2941 gatcgcggta ccgactctcg ttctgtgggg cgaggaggat cacctggttc ccgtcgaggc
     3001 tggccgcatg tgggcgagaa cgatccgcag cgcgagactg atcgtctatc cggcgaccgg
     3061 ccatctgccg caggaagaga tggccgacaa atccgccgcc gatgtacgaa ccttccttct
     3121 cgcaacgagc cggccgtaat ggggcacgca gatcggattg cacattctag cctgcggctt
     3181 ccgtaacgat gcggattcgc gcgagggctt cgttcgcggc ggagagcgcg atgtcgcgcg
     3241 catcgccggt tgccagcacc atcatggcgg agggccgccc gtcgttggag cgcgggaaca
     3301 cgtcgccggg tcgtacggtg acgatcacgt cggcaatgcc gggaatttca cacgccgctt
     3361 caacgccgct gatcgaaacg acgcgtccgg gttgaggaaa cagatagcgt tgcacgacgg
     3421 acgcagtttt cagcggctcc agttcctcca tcgcaactgc atcgccgacg gccaggcgca
     3481 tggcggcgcc gatgaaatcg acgccgctgg agagtgggat cgcgcgcgtg cagagagcgc
     3541 cgccgcaaat cgtcgtggcc agatctatca actgcgcctt gtcgtcatgc acgacgatgc
     3601 cggctttcac cgcaccgctt gttacgccca ggacctgcgc ggcctgcgcg acaagcgctc
     3661 tcgtgtcttt ctggatttca gcaggcaacg cgctcggaag ctccccgcca ttctggacaa
     3721 agaatggcgc gtagcgttcg agatattcga aattgcggtc gaacagcacg ggcgtcatgc
     3781 aatgtccgtc gaggacgagc gactgcgcga agatctgcgg gccgtcgaga caacgctcga
     3841 ccatcacccg gcccgtgatc gaatgggagc gcgccagcgc gaaagcgcga tcgatgtccg
     3901 cgacggctgc caggcgctgc acgccgcggc tctcgcggct gtccaccggc ttgatcacga
     3961 gatctcgccc atgctcgatc acaatgcgtg cgagcgcctg gggcgtttcg acgggcgcaa
     4021 accagggaac cggaaggccg gcttcgcgaa gcgtccgctt catcgcgagc ttgtcgcaag
     4081 caagttgcga gattctttcg gaaggaccgg gcagtccgag gcggcgggcg accgccgcca
     4141 tcgtcatcgg cgcgttcgcc gccatggata tcgcgccatc gatgcggcgg atcttgcggc
     4201 tgaaccgctc cgccgcagcg gaggtctcgt cagcgccatc cgcgtctgca atgagaattg
     4261 aatccgtgac ggcgaaggcc ggcgcctcgg gatcgcgatc ggcgacgatc acgtagaagc
     4321 cgagttcctt cgcgcgtctg gctgcatccg cagcctgcct gccaccggaa atgatgagaa
     4381 gcgccttctg tcccgcctgc gaagcatggt cctgcatgac gtgagaggcc gcgccctgaa
     4441 cgctgcgatg catcgaaatt gtccggtccg gcgcctgcgg ggcactctcc gcatgcccca
     4501 ttaatatttc gctatatgga acggcgcacc atactctctg gggagaaagc gtccgcttgc
     4561 tcctgcctca tagaatcgtg ggctggcgtt gcagaaatcg tttcatcttt cagtcggtgc
     4621 tgaccaagcg ctcctatttt cctccccaac gtgggggagt cggcgcgcgg cttgcgcgcc
     4681 agcccccccc tccgcctccc cccggattta attcggggtc ggcacctccc tcacgttggg
     4741 ggaggaaaaa agagagcaat cgcgcgcgtt aaaccccgca tggacactcg tgaaaaatgt
     4801 gcgctcgcgt tagcctcgtg gggagatcaa atacatccgt aaacggtcca ctttacttgc
     4861 gcgcagactc ctgcctgatc ttcatcaaat gagcgaaccc ccaacaagtc agcgagcgtc
     4921 cgcaacgtca atttcggatg tcgtcgtggc gcgcatcttc tggttcgcgg ttgcgctcgc
     4981 cggagcaagc gccgcttatg ccatcagcgg cgcgcgcgac ctcgtctacg acggctcctt
     5041 ctatctgctc ggcatcgcct cgcatggcgc gtttcagctc ttcgagcccg gccgcgtatc
     5101 ggtgcagctt ctgcagcaag gtttcgcagt catcggcgcg cggctcggca ttcacgattt
     5161 gtggactctc ggccgactct tttcgctcgg tgcatcgggc tggccgatcg tgctgaccgc
     5221 gctttgctgg ttcgtcctgc cgcgcgcgga aaagagctgg atcgccggac cgcttctcaa
     5281 cctcgtgttc gcgatccctg cgacgaactt catcggcatc ggcgagggcg tgatcgcatc
     5341 ctgtctgttg tggcttgcct tcctgctcgc ctggtttcgc atggatcgga gttggggcgc
     5401 cct
//
LOCUS       DASZMC010000515         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2690182,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000515 DASZMC010000000
VERSION     DASZMC010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2690182"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1346)
                     /locus_tag="VG274_10580"
     CDS             complement(126..1346)
                     /locus_tag="VG274_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWE07146.1"
                     /translation="MAQFQYRAMTGSGAVVRGMLDAPSRDQVVRQLRTRGLVPLDASE
                     RGEGGLFGTLAKNLRFGARVTLRGLAMATQELSTLLKAGLELDRALGILVGLSDIGAL
                     RVPFESVRNRVRDGMSFAEALAADPVFPKFYVSMVRAGEHGGTLDVTLQRLTDYLSRT
                     MAIREQITSALVYPSLLLLTAGGSIIVILVFVLPEFEPLFADAGKSLPMATRMVMGAG
                     HVVRDYGWLFVALIVAGVIGLRRALRTPANRLKWDAFVLKWPVLGKLFAAIEVERLMR
                     TFGTLLSNGVPLPTALNLSKDVLSNTVLAAAVKDAAVSLREGERLAQRLARSKVFPAV
                     TLDLIQIGEESGKLDEMLLRQADLDELRIKHKVDRALALLVPVLTITLGMMVAGLIAS
                     MLVAIISVNDLALQ"
     gene            complement(1349..>2982)
                     /gene="gspE"
                     /locus_tag="VG274_10585"
     CDS             complement(1349..>2982)
                     /gene="gspE"
                     /locus_tag="VG274_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HWE07147.1"
                     /translation="AIERAQHLAVESQERLESVLTRLGLVSERDLAEALSQFLHLPLA
                     VAADYPPQPILEEQLGRKFLREAKVLPLREVPEGVVVAMANPLDSYAAHAVRFAVGRP
                     ILPQVALPADLEAAHERLYGDGKSGIHQISGETNEHVGESIADDVDRLKDIASEAPVI
                     RLVNLLITQAVEARASDIHIEPMENELRVRYRVDGVLRPVESPPHRLASAMISRVKIM
                     AKLNIAERRLGQDGRVAMAVRGKDIDLRVATTPTIHGESVVIRILDRAHVELDLDSLG
                     FDESVITPFRALCAHPHGILLVTGPTGSGKTTTLYAALSEINTNDKKILTIEDPVEYQ
                     LQGVNQVQVKPQIGLSFAGALRSFLRHDPDIIMIGEIRDLETAQIAVQAALTGHLILS
                     TLHTNDAATAVTRLLDMGVEDYLLTSTINGIAGQRLLRKLCDACSEEYEPIPELVRRL
                     KLDRLTEGRSIVLRRAVGCAQCHHTGYRGRTSIVEVLTATDAIKQAVLKSTDAGHIQK
                     IAVEEGMQTMATHGLKKALAGTTTIDEVLRVVPVV"
BASE COUNT          546 a          954 c          951 g          531 t
ORIGIN      
        1 caattgcggc gagcaggccc agaatcgcga gcaccacgag cagttccatc aatgtgtaac
       61 cggcctcgtc cttgcgcgtg acggggacgg gaaggcgatt tctccaactc ttcacggatg
      121 aactcctatt gaagcgcgag atcgttcacg ctgatgattg caaccagcat cgatgcgatg
      181 agacctgcga ccatcatgcc cagcgtgatg gtcaggaccg ggacaagcag cgccagcgcg
      241 cgatcgacct tgtgcttgat cctcagctcg tcgagatcgg cctgtcgaag cagcatctcg
      301 tcgagcttgc cggactcctc gcctatctgt atcagatcga gggtcaccgc aggaaacact
      361 tttgagcgag cgagccgctg ggcgaggcgt tcgccttcgc gcagcgaaac cgccgcgtcc
      421 ttgaccgccg cagccaggac cgtattcgaa agcacatctt ttgaaaggtt gagcgctgtc
      481 ggtaacggca ctccgttgga cagcaatgtg ccgaaagtgc gcatcaggcg ctcgacttcg
      541 atggcggcaa aaagcttgcc gaggaccggc catttgagga cgaaggcgtc ccatttgagc
      601 cggttggcgg gcgttcgcag cgcccggcgc agcccgatga cgcccgccac gatcagggcg
      661 acgaagagcc atccgtaatc gcgcaccacg tggcccgcgc ccatgaccat cctggtcgcc
      721 atgggaagcg atttccccgc atcggcgaag agcggctcga actcgggcag gacgaacacc
      781 aggatcacga tgatcgaacc gccggcggtg aggagaagca ggctcggata aaccagcgcc
      841 gacgtgatct gctcgcgaat cgccatcgtg cgcgagagat agtcggtcag gcgctgcagc
      901 gtcacatcga gggtgccgcc atgttcgccg gcgcgcacca tgctcacgta gaatttcgga
      961 aacacgggat cggcggcgag ggcttcggcg aagctcatgc cgtcgcgcac gcggttgcgc
     1021 acgctctcga acggcacgcg caaggcgccg atgtcggaga ggccgaccag gattccgagc
     1081 gcgcggtcga gctcgaggcc ggccttcagc agcgtggaca gctcctgcgt cgccatcgcc
     1141 agaccgcgca gcgtcacccg agcgccgaaa cgaagattct ttgccagcgt tccgaagagg
     1201 ccgccctcgc cgcgctcgga ggcgtccagc ggaacgagcc ccctggtgcg cagctggcgc
     1261 accacctggt cgcgcgacgg cgcatccagc atgccgcgca cgacagcgcc cgaacccgtc
     1321 attgccctgt actgaaactg cgccatcttc agaccaccgg caccacgcgc aacacctcgt
     1381 cgatcgtcgt cgtgcccgcg agcgccttct tgaggccgtg ggtcgccatc gtctgcatgc
     1441 cttcctcgac ggcaatcttc tggatgtggc cggcgtcggt gctcttgagg accgcctgct
     1501 tgattgcgtc cgtcgccgtc aggacttcca cgatcgacgt gcggccccga tagcccgtgt
     1561 gatggcattg cgcgcatccc accgcgcgcc tgagcacgat cgaacgcccc tccgtcagac
     1621 ggtcaagctt gagacgcctc accagttcgg gaatcggctc gtactcttcg ctgcaggcgt
     1681 cgcacaattt gcgcagaagc ctttgcccgg caatgccgtt gatggtcgag gtgagaagat
     1741 aatcttccac gcccatgtcg agcaggcgcg tcaccgccgt cgccgcgtcg ttggtatgaa
     1801 gcgtcgaaag gatgagatgg cccgtcagcg cggcctgaac ggcgatctgc gcggtttcca
     1861 gatcgcggat ttccccgatc atgatgatgt cgggatcgtg acgcaggaac gagcgcagcg
     1921 cgcccgcgaa gctcagcccg atctgcggct tgacctgcac ctggttgacg ccctgcagct
     1981 gatactcgac cggatcctcg atggtcagga tcttcttgtc gttggtgttg atctccgaca
     2041 gcgccgcgta aagcgtcgtc gtcttgccgc tgcccgtggg tccggtcacc agaagaatgc
     2101 catggggatg cgcgcacaag gcacggaagg gagtgatgac gctctcgtcg aagccgagcg
     2161 aatccaggtc gagttcgaca tgcgcgcggt cgagaatgcg gatcaccacg ctctcgccgt
     2221 gaatcgtggg cgtcgtcgcg acgcgcagat cgatgtcctt gccgcgcacc gccatcgcga
     2281 cgcgcccatc ctggccgagc cggcgttccg cgatgttgag cttcgccatg atcttgacgc
     2341 gcgagatcat tgcggaggcc aggcgatgcg gcggcgattc gacgggcctc aagacgccgt
     2401 cgacgcggta gcgcacgcgc aattcgtttt ccatcggctc gatatggatg tccgacgcgc
     2461 gcgcttcgac ggcctgggtg atgagcagat tgacaaggcg gatgacgggc gcttcgctgg
     2521 cgatgtcttt caggcgatcg acatcgtcgg cgatgctttc gccgacatgc tcgttggttt
     2581 ctcccgagat ctggtgaatg ccggatttgc cgtcgccata gagccgctca tgtgcggctt
     2641 cgagatccgc cggaagcgcg acctggggga gaatgggcct gccgacggca aagcgcactg
     2701 catgcgccgc ataggaatcg agcggattgg ccatcgcgac caccacgcct tccggcactt
     2761 cgcgcagcgg caggaccttg gcctcgcgca ggaattttcg tcccaattgt tcttcgagaa
     2821 tcggctgcgg gggatagtcg gcggcgaccg cgagcggcag gtggagaaat tgcgacagtg
     2881 cttcggcgag atcgcgttcg ctgacgagac ccagccgcgt caaaacactc tcgagccgct
     2941 cctggctctc gacagccaga tgctgggcgc gctcgatcgc ct
//
LOCUS       DASZMC010000516         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5447412,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000516 DASZMC010000000
VERSION     DASZMC010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5447412"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..400
                     /locus_tag="VG274_10590"
     tRNA            326..400
                     /locus_tag="VG274_10590"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:358..360,aa:Thr,seq:ggt)
     gene            complement(555..1475)
                     /locus_tag="VG274_10595"
     CDS             complement(555..1475)
                     /locus_tag="VG274_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWE07148.1"
                     /translation="MKRAEVAQYWEANAETWTRHARAGYDIYRDRLNTPAFLAMLQPV
                     HGLSGLDIGCGEGSNTRELARLGARMHAIDVAPTFIRYARDAERAEPLGIAYVVADAT
                     DLPFASRSFDFATAFISMMDMANHGDALTEAARVLRPGGFLQFSILHPCFVPPHRRVL
                     REPDGTTRAIETGGYFDATDGRIDTFRFENVPEEERRKTEPFHVPRFHRTLSGWVHLI
                     VKAGLVIERFGEPRASVEVAKAEPALEDTLVAPPFLHIRVLKPAEPRRKRGSRQRPGE
                     QVARIMPQFWRTISECSGLYLREICTTLPS"
     gene            complement(1695..>2354)
                     /locus_tag="VG274_10600"
     CDS             complement(1695..>2354)
                     /locus_tag="VG274_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWE07149.1"
                     /translation="TPNPALPEVRSAFAERLARQEPPMSFLRAIRAALPADGIYVEEV
                     TQVGFASRLALPVPGPRLFLSPGYQDTLGWGYGTALGAQAAAPGRKVVLATGDGGFMF
                     QAAELATAMRHRLPVVVVVFDDGAFGNVRRIQQQQYGNRLIACDLANPDFVKFAESFG
                     MAAFRAGSPDALETALRQAFALDAPALVHVPVGEMPSPWDMILLPRVRGFADAWRPAM
                     P"
BASE COUNT          440 a          770 c          744 g          400 t
ORIGIN      
        1 gcacgaccgg ttgcgaagga cgtggcggag ccggcggcga gcacggcgga ttaacagtcg
       61 agcccggtca gcgcgcgcaa gcagtggtcg gttcagggtg ctcccgccga actggcagcg
      121 tttgttagtt ggggaagacg agcactggcg ctgactgcct cgcggccatc tgttgagggc
      181 agtctgatcg accttccgcg gtagataggg tgggcggtgc ctttgagccg tccggggcgg
      241 agcctgctgc tcagcagggt tgcgccagtg tagccgacgg tctaggaaca gcccgctggg
      301 aacgagccgt ccccagagcc cgggcgctgc tgtagctcag tggtagagca cccccttggt
      361 aagggggagg tcgagagttc aatcccctcc agcagcacca ttcgctaaat ccacgaaggc
      421 cttcaattcc agagactttt ccggtagcgg tgcgcctccg aaagggggga ttcgccgtgg
      481 gacatggaca gaacacgcca cctttggacc gcgattacgc gacaaatatc gttgagtcgt
      541 tcatgagccg ttcgtcatga tggcaaagtg gtgcaaatct cgcgcaggta caggccagaa
      601 cattcactta tcgttcgcca aaactggggc atgatgcgcg ccacctgctc gcccggccgt
      661 tgccgggaac cgcgtttcct ccgcggctcg gcaggcttga gcactcggat atgcagaaaa
      721 ggcggcgcga ccagcgtatc ttccaatgcg ggctccgcct tggccacttc gacgctggcg
      781 cgcggttcgc caaagcgttc gatgaccagc ccggccttga cgatgaggtg gacccacccg
      841 ctcaacgtcc ggtgaaaccg gggaacatgg aacggctccg tctttcggcg ttcctcctcc
      901 ggtacatttt cgaagcgaaa ggtatcgatc ctcccatcgg tggcatcgaa atagccaccg
      961 gtctcgattg ctcgcgtcgt gccgtcgggc tccctcaaga cgcggcgatg cggcgggacg
     1021 aagcatggat gcaaaatcga gaattgaagg aagccgcctg gtcgcaagac ccgggctgcc
     1081 tcggtcaagg catcgccgtg gttggccatg tccatcatcg atatgaaagc cgtcgcaaaa
     1141 tcgaacgagc gggaggcgaa cggcaaatcc gtcgcatcgg ccacgacata ggcgatgccg
     1201 agcggctcgg ctctctccgc gtcccgcgcg tatcggatga aggtcggcgc gacatctatc
     1261 gcgtgcatcc tcgccccgag tcgcgcgagt tcgcgcgtgt tcgacccctc cccgcagccg
     1321 atatcgagcc ctgagagacc atgcaccggc tgaagcatgg ccaagaatgc gggcgtattc
     1381 aatctgtccc gatatatgtc gtaaccagcg cgggcgtgcc gcgtccaggt ctcggcattc
     1441 gcctcccaat actgcgcgac ttcggctcgt ttcacggaag ccactccgcg gacacactga
     1501 aatctaactc gtcaactaaa cggagagcat tcgatatact ccgtactgcc cgctaagttt
     1561 gcggggcacg atgcgagcgg ccggccagcc ggtcaagccg cggcgcctga aggaacgatt
     1621 ttcaaagtgg cccaacctct cgggcgacac ctctgcgcaa tcgccgggtt cagattgggc
     1681 gtccgaaccg gacctcacgg catcgctggc ctccaggcat ccgcgaaccc gcgcacccgc
     1741 ggcaacagga tcatgtccca cgggctgggc atttccccca ccggcacatg gaccagcgcc
     1801 ggcgcgtcca gtgcgaacgc ctgccgcagc gccgtctcca acgcgtcggg cgagcccgcc
     1861 cggaacgccg ccattccgaa actctctgcg aacttcacga agtctgggtt ggccaggtcg
     1921 caggcgatca gccggttgcc gtattgctgc tgctggatgc gccggacatt gccgaaggcg
     1981 ccgtcgtcga acaccacgac gaccaccggt agccggtgcc gcattgccgt ggcgagttcc
     2041 gctgcctgga acatgaatcc gccgtcgccg gtggccagca cgaccttccg ccccggcgct
     2101 gccgcctgcg cccccagagc cgtgccatat ccccacccca gggtgtcctg atagccagga
     2161 gaaagaaaca gcctgggccc cggcaccggc aaagccagac ggctggcgaa gccgacttgc
     2221 gtgacttcct cgacgtagat cccatccgcc ggcagcgcgg cgcggatcgc gcgcaggaag
     2281 ctcatcggcg gctcctggcg ggccagtcgt tcggcgaagg ccgagcggac ctcgggcagc
     2341 gccgggttcg gagt
//
LOCUS       DASZMC010000517         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_782579,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000517 DASZMC010000000
VERSION     DASZMC010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782579"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VG274_10605"
     CDS             <1..209
                     /locus_tag="VG274_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07150.1"
                     /translation="TLAMQMQFLKSARAGDIVQVQPLLLRRTRVFMFVRGDFTVEDEA
                     IFTAMSTWKLLDQGHIDGDSGSNS"
     gene            348..2180
                     /locus_tag="VG274_10610"
     CDS             348..2180
                     /locus_tag="VG274_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWE07151.1"
                     /translation="MNMAFLSSFRAGRETVYPDSLRDRLRAFPLLKDVGDATLRRLLS
                     EATWFGLPGGTLLARDGENDHALFLVITGSLGVFVEDEKAQRKLVSLVPAGETVGEMS
                     LISGEPHSAQLVALRDSELLRISREGFEALIARHPRVMFNLMRMLVRRLQDATHRPSD
                     GARPKTFAIVPVQEGLADEPIAQRLAAALVEMGSKAAVLDASAAEQSADWFNAFEAAR
                     DVVFYRGDAPDSAWTHLCVRQADKVFLLARADRPLPRRAVDIPAFKARDFGLPELLLL
                     HPTGLTRRLPEHFSLRSGLFESHHHVRAGMNEDIRRLARFVSGRAVGLVLAGGGARGY
                     AHIGVIKALMEAGVPFDRLGGTSMGAIIAAGLAAEWGLDELSARMRDAFVEHSPLSDF
                     TFPLIALLRGKQVSSLLHEHFGEMCIEELPKPFFCVSSDLTSGRIHVHRSGPLWRALR
                     ASVALPGILPPVTHHGHLLVDGGVMNNLPVDVMARQAHGPIIASDVTGELDLRAMDSR
                     YGERPLWWLLWQRMRGTPSIVSILMRSGTVGSEAQRRVVREQADLLFEPPLPDIGLRD
                     WKSLDRAVAEGYAHARTLIEQHGVPISDTWAEGPAVAIPQKEAV"
     gene            complement(2188..2334)
                     /locus_tag="VG274_10615"
     CDS             complement(2188..2334)
                     /locus_tag="VG274_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07152.1"
                     /translation="MSLARNGPALGAQAGEHRLREIVVDKGGFTKFRRATETPFNIIL
                     EARP"
     gene            complement(2508..>3190)
                     /locus_tag="VG274_10620"
     CDS             complement(2508..>3190)
                     /locus_tag="VG274_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWE07153.1"
                     /translation="SSTGKHFSAGMHLSVFKDREQQQERLERGRTGAQLRERLLALQE
                     SFNAIERARMPVLAAIQGGCVGGAVDLVSACDCRYASEDAFFVIQEINLGMTADVGTF
                     PRLCHLIPQGWARELAYTGRRLPAQQAREIGLVNAVLPSHEALLKHVMDVAKDIAEKS
                     PLAVYGSKVMINYARDHTIADGLDYIATWQAGMYNPETDMREALEARQAKRDPQFADL
                     PPRRKDVL"
BASE COUNT          526 a         1024 c         1037 g          603 t
ORIGIN      
        1 tcaccctcgc gatgcagatg cagtttctca aatccgcgcg cgccggcgac atcgttcagg
       61 tgcagccgct gctgctgcgc cggacgcggg ttttcatgtt cgtgcgtggc gatttcacgg
      121 tcgaagacga ggcgatcttc accgcgatga gcacatggaa actcctggac cagggccata
      181 ttgacggcga ttccggcagc aattcataac gccctgttta tcgcgggaat ctaacctctc
      241 caggcgagga gctgggcagg ccggaacgtg gccagcgaga gcggcgggag cgccggggcg
      301 atggcgaatc ggccgtccga tgcgttagaa gggtcgaggg acattcaatg aacatggcgt
      361 tcctttcgtc ctttcgtgcg ggccgcgaga ccgtttatcc ggattcgctg cgcgatcgtc
      421 tgcgcgcgtt tccgctcctg aaagacgtcg gcgatgcgac gttgcgccgg ctgctctcgg
      481 aagccacctg gtttggattg ccgggtggca cgctgttggc gcgcgacgga gagaacgacc
      541 acgcgctatt tctggtgatc acgggttcgc tgggcgtgtt cgtcgaggac gaaaaagccc
      601 agcgcaagct cgtgtcgctc gtaccggccg gagaaacggt gggcgagatg tcgctgatct
      661 cgggcgagcc ccactcggcg cagctcgtcg ccttgcgcga cagtgagctt ttgcgcatca
      721 gccgcgaggg cttcgaggcg ctgatcgcac gccatccccg cgtgatgttc aacctgatgc
      781 gcatgcttgt gcggcgcctg caggacgcaa cacatcggcc gtccgacggc gcgagaccga
      841 agacatttgc catcgtgccc gtgcaggaag gcttggctga cgaacccatt gcgcagcggc
      901 tcgcggccgc gctcgtcgag atgggctcga aagcggccgt gcttgacgca agcgccgccg
      961 agcaatcggc ggactggttc aacgcattcg aggcggcgcg cgatgtcgtc ttctatcgcg
     1021 gcgacgcgcc cgacagcgcc tggacgcatc tctgcgtgcg acaggcggac aaggtctttc
     1081 tgctcgcccg cgccgacagg cccttgccac ggcgcgccgt cgacattccg gccttcaagg
     1141 cgcgcgattt cggcctgcca gagcttctgc tgctccatcc gaccggactg acgcgccgtc
     1201 tgccggagca tttctcgctt cgcagcgggc tcttcgaatc gcaccaccat gtgcgcgccg
     1261 gcatgaacga agatatccgc cggctcgcgc gctttgtatc cggacgcgcg gttggcctcg
     1321 tgctggcggg aggcggcgca cgtggttacg cccatatcgg cgtcataaag gcgctgatgg
     1381 aagccggcgt ccccttcgac cgtctgggcg gcaccagcat gggtgcgatc atcgccgccg
     1441 gccttgcggc ggaatggggc ctcgacgagc tgagcgcgcg catgcgcgac gccttcgtcg
     1501 agcacagccc gctttccgat ttcaccttcc cgctcatcgc tttgctgcgc ggcaagcagg
     1561 tatcgagcct gctgcacgaa cattttggcg aaatgtgcat cgaggagttg ccgaagccgt
     1621 tcttctgcgt ctcctccgat ctcacttcgg gtcgcattca tgtgcatcgc tccggtccgc
     1681 tgtggagagc gctacgcgcc agcgtggctt tgcctggaat cctgccgccc gtgacccatc
     1741 acgggcatct gctggtcgac ggcggcgtga tgaacaatct gccggtcgac gtgatggcca
     1801 ggcaggcaca cggacccatc atcgcttcgg acgtcacggg cgaactcgat ctccgcgcca
     1861 tggattcgcg ctatggcgag cgcccgctct ggtggcttct ctggcaacgc atgcgcggca
     1921 cgccttcgat cgtctcgatc ctgatgcgct ccggcacggt gggttcggaa gcgcagcggc
     1981 gcgtcgtgcg cgagcaggcc gatctgctct tcgaaccgcc gcttccggat attggtctgc
     2041 gcgactggaa atccctcgat cgcgccgtcg ccgaaggcta cgcgcacgcc aggacgctca
     2101 tcgagcagca tggcgtaccg atcagcgaca cctgggccga aggcccggcc gtcgccatcc
     2161 cgcagaaaga agcggtgtag agagtcctta cggtcgcgct tccaggatga tgttgaaggg
     2221 cgtttcggta gcgcggcgga atttcgtaaa gccccccttg tcgaccacga tttcgcgcag
     2281 ccgatgttcg ccggcctgag cgccgagcgc cggcccgttg cgggccagcg acatgggaac
     2341 gcagaccagc gccgatgctc cgtctgagtg taatgaagat tcgccgcttc gtgcgaaagg
     2401 ccggccggcc ccaatgatcc acttcactca cgccgtcgag cgcgagggcg aatcgtctca
     2461 catcgccaca gatcgacatg cgagcacgcc ggtggaggaa aaccctctca caatacgtct
     2521 ttgcgccttg gcgggaggtc ggcgaactgt ggatcgcgct ttgcctgccg cgcctcgaga
     2581 gcctcgcgca tgtcggtctc gggattgtac atgccggcct gccaggtcgc gatgtaatcg
     2641 agaccatcgg cgatcgtgtg gtcgcgggcg tagttgatca tcaccttcga gccgtacacg
     2701 gcgagcggcg atttctctgc gatgtctttc gccacgtcca tgacgtgttt gagcagcgct
     2761 tcgtgcgagg gcaacacggc gttgaccaga ccgatctctc gcgcctgctg cgccggcaac
     2821 cgccggcccg tataggcgag ctcgcgcgcc cagccttgcg ggatcagatg gcagaggcgc
     2881 gggaacgtgc cgacatcggc ggtcatgccg agattgattt cctgaatcac gaagaatgcg
     2941 tcttcgctcg cgtagcggca atcgcaggcg gacacgagat cgactgcgcc gccgacgcac
     3001 ccgccctgga ttgcggccaa cacgggcatc cgggcccgct cgatcgcatt gaaagactcc
     3061 tggagcgcga gaagtctttc gcgaagctgc gcgcccgtcc gcccgcgttc gagcctttcc
     3121 tgctgctgtt cgcggtcctt gaacaccgag agatgcatac cggcggagaa gtgtttgccg
     3181 gttgatgaga
//
LOCUS       DASZMC010000518         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2420514,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000518 DASZMC010000000
VERSION     DASZMC010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420514"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..531)
                     /locus_tag="VG274_10625"
     CDS             complement(109..531)
                     /locus_tag="VG274_10625"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HWE07154.1"
                     /translation="MRWFSPFTLCAALLALPSPAAATGIFGLWATEKNDGRVLIELCG
                     KAICGRVVDGNQLRANPDQMDVNNPDPAKRTRRVKGLTILEGYTGSPPQWSGGSVYDP
                     QTGDETHDSTLILVAPDTLKVEGCRIVFCRSETWTRTN"
     gene            complement(518..1369)
                     /locus_tag="VG274_10630"
     CDS             complement(518..1369)
                     /locus_tag="VG274_10630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWE07155.1"
                     /translation="MAETQSPACLAIHPRDIAFGRASSPSPPRWWLGGDPIATAFFNA
                     LSASFPQGERFFMDAVRLYRGRVDQRLGEQIQAFIAQEAVHSREHLVFNNKMAAQGFD
                     FGRIDSYLKKRFDFGRTLPPLNRLCATIALEHFTAILAHAILADGDDLAGAPPELKRM
                     WQWHAIEEIEHKAVAFDTFLAAARHFSPFRRWQLRCVSMALTTIMFLQFVGFGVREFL
                     RHDGIDNLRSWRALFRYLWIGPGIMRRVAPSYFVYYKPGFHPWRVDDRALIADVEATL
                     TPGHAVV"
     gene            1436..>1620
                     /locus_tag="VG274_10635"
     CDS             1436..>1620
                     /locus_tag="VG274_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWE07156.1"
                     /translation="MRKPKPRPAPRRRRSADEARGEALASARKLLIERGPGAVTLKAV
                     ADDLGMTHTNLLHHFGTA"
BASE COUNT          326 a          496 c          517 g          281 t
ORIGIN      
        1 cagaaaatgg tgcagcccgc acaggaattc cacccggttc ttgcggatca atgtggtgct
       61 cacgcgccat ggacattccg cggtgacgat cgtcccgttt ttcccggatc aattcgtgcg
      121 ggtccaggtt tcggagcggc agaagacgat gcggcagcct tcgactttga gcgtgtcggg
      181 cgcgacgagg atcaaggtcg agtcgtgcgt ctcatcgccg gtctgcggat cgtagacact
      241 gccgccactc cattgcggcg ggctcccggt gtagccttcc aggatcgtga ggcccttcac
      301 gcgccgcgtc cgtttcgccg gatcgggatt gttcacatcc atctgatccg ggttggcgcg
      361 cagctggttg ccgtcgacca cgcgtccgca aatcgctttg ccgcacagct ctatgagcac
      421 gcgtccgtca tttttctcgg tcgcccaaag tccaaaaatg ccggtcgccg cagccggaga
      481 cggcaacgcg agcagcgcgg cgcacaaagt gaacggacta aaccaccgca tggcccggtg
      541 tcagtgttgc ttccacatcg gcaatcagcg cgcggtcgtc aacgcgccag ggatggaagc
      601 ctggcttgta gtagacgaag tagctgggcg ccacgcgccg catgataccg ggcccgatcc
      661 agagataccg gaaaagcgcg cgccagcttc gaagattgtc gatgccgtcg tggcgtaaga
      721 actcgcgcac gccgaacccc acgaactgca ggaacatgat cgtggtcaac gccatcgaga
      781 cgcatcggag ctgccagcgc cggaaaggtg agaagtgccg cgcagcggcc agaaacgtat
      841 cgaaggcgac ggccttgtgc tcgatctcct cgatggcgtg ccactgccac atgcgcttca
      901 gttcaggcgg cgcaccggca agatcgtcgc catcggcgag aatcgcatgc gcgagaatgg
      961 cggtgaaatg ctcgagcgcg atggtggcgc aaagccggtt gagtggcgga agcgtgcggc
     1021 cgaaatcgaa tcgcttcttg agatagctgt cgatacggcc gaaatcgaat ccctgggcag
     1081 ccattttgtt gttgaagacg aggtgctccc ggctatgcac ggcttcctgc gcaatgaagg
     1141 cctggatttg ctcgccaagc ctctggtcca ctctgccgcg atagagccgc acggcatcca
     1201 tgaagaagcg ttccccctgg ggaaatgagg cggacagcgc gttgaagaag gccgtggcga
     1261 tcggatcgcc gccgagccac cagcgtggag gggaagggga agatgcgcgc ccgaaggcga
     1321 tatcgcgggg atggatggcc agacaggccg gcgattgcgt ttcagccacg aaagcgtgat
     1381 cctctgactg tcgaccctga tggtagttaa actgacaacg atatcaatac gcaggttgcg
     1441 aaagcccaag ccgcgccccg cgccgcggcg tcgccgctcg gccgacgaag ctcgaggcga
     1501 agctctggcc tcggcgcgca agctcctgat cgagcgcgga ccaggcgccg tcacgctcaa
     1561 agccgtggcc gacgacctcg gcatgacgca caccaacctt ctccatcact tcggtacggc
//
LOCUS       DASZMC010000519         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4889631,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000519 DASZMC010000000
VERSION     DASZMC010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4889631"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VG274_10640"
     CDS             complement(<1..806)
                     /locus_tag="VG274_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWE07157.1"
                     /translation="MSLIDALIKALAMAFAMAWEILWPLILGFALSAVVEAVVSHKQM
                     GRLLPDDRPRSIAVAIALGAASSSCSYAAVALARALFRRGADFTAAMAFELASTNLVA
                     ELSIVLIVFLGWTFMAAEAVGAFVMVAILVVLFRAFLTPGLLAEAKQQADRGIDGRME
                     GHAAMDMSVTEGGSLWRRITSPQGLTAISHYFVMDWASVWMDITGGLLIAGALAASVP
                     PSFWQGLFLTGHPILATIWGPIVAPLVAIISFVCSVGNIPLAAVLWNGGI"
     gene            complement(934..1551)
                     /locus_tag="VG274_10645"
     CDS             complement(934..1551)
                     /locus_tag="VG274_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWE07158.1"
                     /translation="MVDHGDDILEEAFGRGPERELAHGESLFRQGDRAAAIFLIVRGR
                     LRMIRRLATGAQVTIHTGRAGEMFAEGALFSEAYQCDAIAAEATQVRMCAKAELLAAA
                     KRSPTTMLAILERVARQLHHARTMRELRNILSAEERALEHLRLSLPRGGAGDVVVFDR
                     PLMEIADDLGLAHEAYYRALAALASQGRIERKGRTIILLAEAGKR"
     gene            complement(1551..2327)
                     /locus_tag="VG274_10650"
     CDS             complement(1551..2327)
                     /locus_tag="VG274_10650"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HWE07159.1"
                     /translation="MLTRRHVLQAGLTTGCSVCAALAFGTMSRGLAAPATAPTEIDGP
                     GYRLRFIGSQRETIMMGNRAALLDLRTLKGQPHLYGIGPIEGLSGEVAIADSRPSLAR
                     VGADKRAHVEESYEAGAPFFVWAEVPRWQDAPMPAQVRSFADLEAFIAEAGKTAALTQ
                     AFPFVVRGRPALIDFHIMDAKPETPPGMAEHQKMQIPFELHTRQATLVGFWSNQHRGI
                     FTPMGTNIHIHFQTPENDTSGHVQGLDLGQGGMVLSLPKG"
     gene            complement(2415..>3050)
                     /locus_tag="VG274_10655"
     CDS             complement(2415..>3050)
                     /locus_tag="VG274_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWE07160.1"
                     /translation="LPAHGEGNAARGARVFGACAACHALQPDRNITGPSLPGLWNRKA
                     GSLPSFERYSSALKGANIVWDDATLDAWLADPQHLVPGNQMTFAGIKDAPARADLLAY
                     LKQATKPGAQLAQQPRGMMGGGAVPNLKKLDAEDRVQAIRYCRDTYRVTTADGKTHVF
                     WERNLRLKTDSSSDGPEKGAPALVGAGMAGDRADVIFADPAEISTSIVPNC"
BASE COUNT          547 a          988 c          987 g          528 t
ORIGIN      
        1 ctgatgccgc cgttccaaag cacggcggcg agcgggatgt tgccgaccga gcagacgaac
       61 gagatgattg ccaccagcgg agcaacgatc ggaccccaaa tcgtggccag tatggggtga
      121 cccgtgagga acaggccctg ccagaaggag ggcggcaccg acgcggccag cgcgcccgcg
      181 atcaaaaggc cacccgtaat gtccatccat accgaagccc aatccatcac gaagtaatga
      241 ctgatcgccg tcaggccctg gggcgaggtg atcctccgcc acagcgaacc gccttcggtt
      301 accgacatgt ccatcgcggc gtggccttcc atgcggccgt cgatgccgcg gtcggcctgt
      361 tgcttcgctt cggccaggag cccgggcgtg aggaaagcgc gaaacagcac aacgaggatc
      421 gccaccatga cgaacgcgcc gacggcctcg gccgccatga acgtccagcc gagaaacacg
      481 atcaggacga tgctcagctc ggcgacgaga ttggtcgagg ccagctcgaa cgccatcgcg
      541 gccgtgaaat ccgcgcccct gcgaaacagc gcgcgcgcca aagcgaccgc cgcgtaggag
      601 caggacgacg aggcggcgcc gagtgcgata gccacagcta tcgagcgtgg ccggtcatcg
      661 ggcaacaaac ggcccatttg cttgtgcgag acaacggcct cgacgacggc ggagagcgca
      721 aagccaagga tcagcggcca caggatttcc caggccatgg cgaaggccat cgcgagggct
      781 ttgatgaggg catcgatcag agacattcag gacctcgcta cgctgatcct cattttatct
      841 ttcggtaggg ctcgagacgt gcgccacaca ccggaatcat gctcctgaac tgagcatact
      901 gcaccaagat tcgcgggctg ggtcggcgcg cgcttagcgc tttcccgctt cggccaggag
      961 aatgatcgtc cggcccttac gctcgatcct gccctggctc gccagggcgg cgagcgcccg
     1021 ataatacgct tcatgggcca gacccagatc gtccgcgatt tccatcaatg ggcgatcgaa
     1081 tacgacgaca tcgcccgcgc cgccccgcgg cagtgatagc cgcaggtgtt caagcgcgcg
     1141 ttcctcggct gaaaggatgt tgcgcaattc gcgcatggtg cgcgcatggt gaagctggcg
     1201 cgccacgcgt tccaggatgg caagcatggt ggtcggagag cgcttggccg ccgccagcag
     1261 ttcggccttg gcgcacatgc gcacctgcgt cgcctcggcg gcgatggcgt cgcattgata
     1321 ggcttccgag aacagcgcgc cctcggcaaa catctcgcct gcgcgccccg tgtggatcgt
     1381 gacctgggct ccggtggcga gacgccggat catgcgcagg cgcccgcgca cgatcagaaa
     1441 tatcgcggcc gcgcggtcac cttgacgaaa aagcgattcg ccgtgcgcca gctcacgctc
     1501 ggggccgcgc ccaaacgctt cctctaaaat gtcgtcgccg tgatcgacca tcatcctttc
     1561 ggcaagctga gcaccatgcc gccttgcccg agatcgaggc cttgcacatg gcctgacgta
     1621 tcattctcgg gggtctggaa gtggatgtga atattcgttc ccatgggcgt gaagataccc
     1681 cgatgttggt tcgaccagaa gccgacaagg gtcgcctgcc tcgtgtgcag ctcgaaggga
     1741 atctgcattt tctgatgctc cgccatgccg ggcggcgtct cgggttttgc gtccatgatg
     1801 tggaaatcga tcagcgcagg ccggcctcgc acgacgaacg ggaacgcctg ggtcagcgcg
     1861 gccgtcttgc cggcctccgc aatgaaggct tccagatctg caaagctccg tacctgcgcg
     1921 ggcatgggtg catcctgcca gcgcggcact tcggcccaca cgaagaaggg cgctccggct
     1981 tcataggatt cctcgacgtg cgcgcgcttg tccgcgccca cccgggcgag cgaaggccgg
     2041 ctgtctgcga tcgctacttc gccgctcagg ccctcgatcg ggccaatacc gtagagatgc
     2101 ggctgtccct tgagggttcg caggtcaaga agcgcggcgc ggtttcccat catgatcgtt
     2161 tcgcgctggc tgccgatgaa ccgcaggcgg tagcccggtc catcgatctc ggtcggtgcc
     2221 gtcgccggag cggcgaggcc gcgcgacatt gttccgaaag caagcgcggc gcaaacggag
     2281 cagccagtcg taagaccggc ttgaaggaca tggcgtcggg tcagcatgga ggtctcctgg
     2341 gttgcccgca ccctgcgctg tctgcgccgc tgcctcctat gatccggatc atgtcgcgcg
     2401 gccggaggat cgattcaaca gttcggcacg atcgaagtgc tgatttccgc tggatcggcg
     2461 aaaatcacat cggcgcggtc gcccgccatc ccggcgccga cgagagcggg agcgcctttc
     2521 tcgggaccat cactgctgct gtcggtcttg agccgaagat ttcgttccca aaacacgtgc
     2581 gtcttgccgt cggccgtcgt cacccggtag gtatcgcggc aatagcggat cgcctgcacg
     2641 cggtcttcgg cgtcgagctt cttgaggttc ggcaccgcgc ccccgcccat catgccgcgc
     2701 ggctgctggg cgagctgcgc tcccggcttc gtcgcctgtt tgaggtaggc aagcaggtcg
     2761 gcgcgtgctg gcgcatcctt gatgccggca aatgtcatct ggttgccggg gaccaggtgc
     2821 tgcgggtcgg cgagccatgc atcgagcgtg gcatcgtccc acacgatatt tgctcccttc
     2881 agggcggacg agtaacgctc gaaactgggc aggctgccgg ctttgcggtt ccagaggccg
     2941 gggaggcttg gccccgttat gttgcggtcc ggttgaagcg catggcacgc cgcacaggca
     3001 ccgaacaccc gtgccccgcg cgccgcgttg ccttcgccat gcgccgggag
//
LOCUS       DASZMC010000520         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3763113,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000520 DASZMC010000000
VERSION     DASZMC010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3763113"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VG274_10660"
     CDS             <1..253
                     /locus_tag="VG274_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07161.1"
                     /translation="KVYVRGAIYGNLTVDPGGRVHVFGLIQGNLMVHKGAKVIHSGSV
                     GGDAINLGGRLYIEKDAKVSGKIKAKKGETRVNGHLMEE"
     gene            complement(326..841)
                     /locus_tag="VG274_10665"
     CDS             complement(326..841)
                     /locus_tag="VG274_10665"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWE07162.1"
                     /translation="MNVVLVGYRGSGKTTIGRLLAERLWVNFVDLDEMIVARAGKSIK
                     AIFDELGENGFRDLETNAIAEAVKETDTVLALGGGALGREENRALLRSEHFRIIYLRC
                     DAEELLRRITADPQTALARPSLTRLGGSLEEVATLLAQREPIYREMMHGELDVTHLTP
                     REAVVHIARMV"
     gene            1096..1890
                     /gene="pyrH"
                     /locus_tag="VG274_10670"
     CDS             1096..1890
                     /gene="pyrH"
                     /locus_tag="VG274_10670"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWE07163.1"
                     /translation="MPQPTQSLDIKQQIGATTDGAPPAYKRVLLKISGEGFCHEGGFG
                     IEAPELENIARQCVEVAKMGVQLAIVVGGGNFIRGATFAEDGNIPRATADYMGMLATV
                     LNAVALQETMEKFGQPTRVQSAISVYSVCEPFIRRRAVRHLEKGRALILAAGTGNPFF
                     TTDTCAALRATEIQADVLLKATKVDGIYSADPKKDASATLYHDITYPQVLAQQLRVMD
                     LTAITLCMERKIPLVVFNLKRDGNIARVMRGEKVGTKIVPQDGDLK"
     gene            1908..>2444
                     /gene="frr"
                     /locus_tag="VG274_10675"
     CDS             1908..>2444
                     /gene="frr"
                     /locus_tag="VG274_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWE07164.1"
                     /translation="MPADDILFDAEEAMEKAMEHLKHELRGIRTGRATPALVEHIKVD
                     YYGSPTDLKSIASISVPEAKQLLIKPFSPGDLKAIEKAINDSKVGLTPHSDGKQLRLM
                     LPPLSQERRLQLAGQCKGFAETAKIAIRNARRDANKLIDTEHKGSVMTEDQANAAKEQ
                     VQELTKATEGKIDELIEHK"
BASE COUNT          542 a          787 c          703 g          412 t
ORIGIN      
        1 caaggtttac gtgcgcggag ccatctacgg caatctcacc gtcgatcccg gcggtcgagt
       61 acacgtcttt ggactcatcc agggaaatct gatggtgcat aaaggggcca aggtgatcca
      121 cagcggcagc gtcggcggcg acgccatcaa cctcggcggc cgtttgtaca tcgaaaagga
      181 cgcgaaagtg tccggcaaaa tcaaagccaa gaagggcgag acccgcgtca atggacatct
      241 gatggaggag tgacttcggg aagcacgctc cccacgacaa cgggtccgcc agagacggcg
      301 agtggaggga aagccaccgc gacgctcaca ccattcgcgc aatatgcacc accgcctccc
      361 gcggcgtcag gtgcgtcaca tccagttcgc cgtgcatcat ctcccggtag atcggctcgc
      421 gctgcgccag cagcgtagcg acttcttcga ggctgcctcc caggcgggta aggcttggcc
      481 gggccagcgc cgtttgcgga tcggcggtaa ttcgccgcag caattcctcg gcatcgcagc
      541 gcaggtagat gatgcggaaa tgttcgctgc gcagcagggc gcggttttcc tcgcgaccca
      601 gtgccccgcc gcccaaagcc agcactgtgt cggtctcctt gaccgcctcg gcgatggcgt
      661 ttgtctcgag atcgcgaaat ccgttttcgc caagttcgtc aaaaatggct ttgatgctct
      721 tgccagcgcg agcgacgatc atttcgtcaa gatcgacaaa gttgacccac aagcgctcgg
      781 caagcagcct gccgatggtg gtctttcccg acccgcgata gccaaccaaa acgacgttca
      841 ttggcgagat tatcgcaata tgtggggtgc aagtcgattg tgtgccccgc ttcgactccg
      901 cgagctaccg ctcctggcta tcaaagagat tttgccgccc caacttcacc tctccaaaag
      961 ccggcgtctg agggtagtcc cgccggattc ttctcgcgtc gcgcctgccc cggggaaatt
     1021 tagccgcgcc ccaaatcggt tcgcgtcgaa tcacatctgg taactgccgt cgcgcccccg
     1081 tattattcag gccatatgcc ccagcccacg caaagcttag acatcaagca acagatcggc
     1141 gcaaccacag acggcgcgcc gccggcgtac aagcgcgtcc tgctgaagat cagcggtgaa
     1201 ggattctgcc acgagggcgg gttcggcatc gaagcgcccg agctcgagaa catcgcccgc
     1261 caatgcgtcg aagtcgccaa gatgggcgtg caattggcta tcgtcgtcgg cggcggaaac
     1321 ttcattcgcg gggccacctt cgcggaagat ggcaacatcc cgcgcgccac cgccgactac
     1381 atgggcatgc tggccacggt gctcaatgcg gtggcgctgc aggaaacgat ggaaaagttc
     1441 ggccagccga cgcgggtgca gtcggcgatc agcgtctaca gcgtctgcga gccgtttatc
     1501 cgacgccgcg ccgtacgcca tctggaaaaa ggccgtgccc tgattctggc ggccggcacg
     1561 ggaaacccgt tcttcaccac cgacacctgc gccgcgctgc gggctaccga gattcaggcc
     1621 gatgtgctgc tcaaagccac caaagtggac ggcatttatt cagccgaccc caagaaagac
     1681 gccagcgcca ccctgtacca cgacatcact tatccgcagg tgcttgccca gcagcttcgg
     1741 gtgatggatc tgacggccat taccctgtgc atggagcgga agatcccgct ggtggtgttc
     1801 aacctcaaac gcgacggcaa tatcgcccgg gtcatgcgcg gcgaaaaagt gggcaccaaa
     1861 atcgtccccc aggacggcga cctcaaataa aatatctgga gtaaactatg ccagccgatg
     1921 acatcctttt cgacgccgaa gaagccatgg aaaaagccat ggagcacctc aagcacgagc
     1981 tgcgcggcat tcgcaccggc cgcgccacgc cggcgctggt cgagcacatc aaggtcgact
     2041 attacggatc ccccaccgac ctcaaatcca tcgcctcgat cagcgtcccc gaggccaagc
     2101 aactgcttat caagcccttc agccccggcg atctcaaggc catcgagaag gcgatcaacg
     2161 acagcaaggt gggcctgacg ccgcacagcg atggaaagca gcttcgcctg atgttgcccc
     2221 ccctctcgca ggagcggcgt ctgcaactgg ccggccagtg caagggtttc gccgagaccg
     2281 ccaagatcgc catccgcaat gcccgccgcg atgccaacaa gctgatcgac accgagcaca
     2341 aaggcagcgt catgaccgaa gaccaggcca atgccgccaa ggaacaggtc caggagttga
     2401 ccaaagccac ggaaggcaag atcgacgaat tgatcgagca taag
//
LOCUS       DASZMC010000521         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_518648,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000521 DASZMC010000000
VERSION     DASZMC010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518648"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(561..1163)
                     /locus_tag="VG274_10680"
     CDS             complement(561..1163)
                     /locus_tag="VG274_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07165.1"
                     /translation="MRRLISVVVAMLAVALTAVSNSWPQEPKRPTIRSLPPNGQAWRI
                     EKIADIPPPLKSAIDRTQCRLEEYILHESPIQIFRIGRTVIGLVTCQGIIWYGRAFVF
                     EHDPSVEPKPVLFPILASPSGVGTSEMPGYLAWDASTKILTATQGNDVGGGDEFRHVY
                     RYDGRLFTLIRVETQKCCRASADEAWTPIWDAPAWPPPPR"
     gene            complement(1160..>1520)
                     /locus_tag="VG274_10685"
     CDS             complement(1160..>1520)
                     /locus_tag="VG274_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HWE07166.1"
                     /translation="TIGHNFEAVIADLEARMREAAADLNFEEAARLRDEVKRLRATEL
                     AVVDDPTAKRPALPSPARGGSAREARRGGVTARATGPRKPNLDEMGPGPESAPYRGPR
                     STLGRPGMRGGFKRKGR"
BASE COUNT          246 a          473 c          503 g          298 t
ORIGIN      
        1 aggccagccc cggatgcgtg caccgctgaa tagcggggat agatggaatc ctgcttgcgt
       61 ttccggcgca agtggcgcat ggcagtaagt gcagagtgac attggcgcgt cctgagcccg
      121 cgcacgcttt gctactcccg aaggtcgtcc gaaaatctac gaacaaaact cgcgcctcac
      181 cggccgcgcc tcgcgcgcgc cgccgcccac tgccatccgc gttacaaccg aacattgtga
      241 actcgcacgg atttttcatt gtttccattg ttgttcgcat ttgttctttc attgcttccc
      301 attgttgttg cgctcacggc gtttctaccg cctttgcaag gccttacatt gcagatggcg
      361 tcgattttcc attgcagatc gattgttttt attttggtat accctggcgg cgcgttcgga
      421 cgagctgggg gagcggcgtc ccttgagacg ctacggccgg ctggtgacgc aggcgcgcgg
      481 cggccgagtt ggcagaacgg cagaacaaag ccatggctgg aaagctcagt agttgcagcg
      541 aggttctcga tccagcgtca ctatcgaggc ggcggcggcc acgcgggcgc atcccatatc
      601 ggtgtccacg cttcgtcggc ggaggcgcgg cagcattttt gcgtctcgac gcggatcagc
      661 gtgaacaagc gtccgtcgta gcgatagacg tggcggaact cgtcgccgcc gccgacatcg
      721 ttgccctgcg ttgccgtcag tatcttggtg ctcgcgtccc aagcgagata gccaggcatc
      781 tcggaggtgc caactccgga tggcgacgca aggatcggaa acagcaccgg cttgggctcg
      841 acgctcgggt cgtgttcgaa cacgaaggcg cggccgtacc aaatgatccc ctggcacgtg
      901 acgagtccga tcaccgtccg gccgattcga aagatctgga tcggagattc gtgcaggatg
      961 tattcctcga gccggcattg ggtgcgatcg atggcgcttt tcagcggcgg cggaatgtcg
     1021 gcaatcttct cgatgcgcca cgcctgtcca ttgggtggaa gcgacctgat ggttgggcgc
     1081 ttcggctcct gaggccacga attcgagacc gcggtcaggg cgaccgccag catcgccacg
     1141 acgacgctga tgagccgcct catcggccct tgcgcttgaa gccgccgcgc atccccgggc
     1201 ggccgagggt ggagcggggg ccgcgatagg gcgcggactc cggaccgggc cccatctcgt
     1261 cgaggttggg cttgcgtgga cccgtggcgc gcgcggtcac cccaccccgg cgcgcttcgc
     1321 gcgccgaccc tccccttgca ggggagggta aagcgggccg cttcgcggtc gggtcgtcga
     1381 ccacggcgag ctcggtcgcg cgcaggcgct tgacctcgtc gcgcaggcgg gcggcttcct
     1441 cgaaattgag atcggcggcg gcctcgcgca tgcgcgcctc gaggtcggcg atcaccgcct
     1501 cgaaattgtg gccgatggtg
//
LOCUS       DASZMC010000522         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5448907,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000522 DASZMC010000000
VERSION     DASZMC010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5448907"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VG274_10690"
     CDS             <1..285
                     /locus_tag="VG274_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07167.1"
                     /translation="AGCPSLTKPSSPKDAIVRLVLKGTVVNLEIKKPIGKWEKISGAP
                     YAKIEADPETGFPRGLGLDTISGFTTLMQDNKNRMVFAKMSEPAKSSNSE"
     gene            complement(319..453)
                     /locus_tag="VG274_10695"
     CDS             complement(319..453)
                     /locus_tag="VG274_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07168.1"
                     /translation="MMVADNGSNWYISGAPANSHLVDELSQVQASNFEVVRMKGIVTP
                     "
     gene            845..1375
                     /locus_tag="VG274_10700"
     CDS             845..1375
                     /locus_tag="VG274_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HWE07169.1"
                     /translation="MNEKDAKDAKARRTAPLRTPNELGANATKDISGALTTLLADAYT
                     LYLKTKNFHWHMSGPHFRDYHLLLDEQADQIFAITDPIAERVRKLGGTTLRSVGHMAR
                     LQRLRDNDAEYVTPLDMLAELREDNKQLTGYMRETHGLCDEHGDVATASLLEIWIDEA
                     ERRTWFLFEAGRRGES"
     gene            complement(1386..1856)
                     /locus_tag="VG274_10705"
     CDS             complement(1386..1856)
                     /locus_tag="VG274_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWE07170.1"
                     /translation="MPGIVRRVGKCPRSRKWRPILLFCIPLLACSPQAEPETTKSVES
                     VLLHQEAAWNRGDIAAFMSGYWKSPGVRFLSGDKIVSGWNETLARYRAKYATRAQMGI
                     LGFSDLRVDMLAPDAALVVGRWRLDRAGDRPHGVFSLTFRKISGRWVIVLDHTS"
BASE COUNT          426 a          626 c          602 g          322 t
ORIGIN      
        1 gccggttgcc ccagcctgac caaaccatcg tcgcccaaag atgccatcgt ccgactcgtg
       61 ctgaagggca ctgtcgtgaa cctcgagatc aagaagccga tcggcaaatg ggagaaaatt
      121 tctggcgcgc cctatgcgaa gatcgaagcg gatccggaga cgggattccc gcgcggcctt
      181 ggcctcgaca cgatttccgg tttcacgacg ctgatgcagg acaacaagaa ccgcatggtg
      241 ttcgcgaaga tgtcggaacc agccaaatcc tccaattcgg agtgaccccc cggccgcaac
      301 gcactcgaag ggtgccgctt acggcgtcac tattcccttc atcctgacga cctcgaaatt
      361 cgaggcctga acctgcgaca gttcgtcgac aaggtgcgag tttgcaggtg cgccggagat
      421 gtaccagttg ctgccattgt ccgcgaccat catgccgtat ctctgcagcg ccttgattgt
      481 cggccggaaa cggcgccgcg ccgttgagac ttgcgccgag gctcgcgttc gccatcgatc
      541 cgtattgcca gaccggacgt agtgcagatc ccgcgagtgc cggcgcgaat gcgatcatgg
      601 cagccgaaac tgcggcgatc gacagggcaa cacgaagaag cgtcatcatc tcgcgctcca
      661 gccgagcact cgccccgcgc ggcgcctttc ccgaaaccca ggatgccggg agagccggac
      721 ccggcagcct cacgcatcgg ctgcagattt ccatgccgcg gcaacattca ggttagaaaa
      781 gtgttgccgc aggagcggca atttgtgcga atatcgcatc ccccaacgca agagcgagac
      841 tgccatgaac gagaaggacg caaaagacgc aaaagcacgg cgaaccgcgc cgctgcgcac
      901 acccaacgag ctaggcgcca atgcgacgaa ggatatatcc ggggcactga cgacgctcct
      961 cgccgacgcc tacacactct acctgaagac gaagaacttt cactggcaca tgtccggccc
     1021 tcacttccgc gactatcacc tgttgctgga cgaacaggcc gaccagattt tcgccatcac
     1081 cgatcccatc gccgaacgcg tccgcaaact cggcggcaca actttgcgct cggtgggcca
     1141 tatggcgcgc ctgcagcggc tgcgggacaa tgatgcggaa tacgtcacgc cgctggacat
     1201 gctcgcggag cttcgcgagg acaacaagca gctgaccggc tacatgcgcg aaacgcatgg
     1261 gctgtgcgac gagcatggcg acgtggccac cgcgagtctg ctggaaatct ggatcgacga
     1321 agcggagcgg cgtacctggt ttctgttcga ggcgggccga aggggcgagt cctagccacg
     1381 caaactcacg aagtgtgatc gagcacgatc acccagcggc cggaaatctt gcggaaagtc
     1441 agcgagaaga cgccgtgggg ccggtcacct gcgcgatcga gccgccatcg gccgacgacg
     1501 agcgccgcgt cgggggcaag catgtccacg cgaagatcgg agaagccgag gatgcccatc
     1561 tgcgcgcgtg ttgcgtattt cgcgcggtag cgtgcgagcg tttcgttcca tcccgagacg
     1621 atcttgtcgc cagagagaaa acgcacgccc gggctcttcc agtagccgga catgaacgcc
     1681 gcgatgtcgc cgcgattcca cgccgcttcc tggtgcaaaa gaacggattc gacggatttc
     1741 gtggtttcgg gctcggcttg cggagagcag gcgagaagag ggatgcagaa caggagaatg
     1801 ggccgccact tacgcgaccg cggacatttc ccgacccgcc tgactattcc gggcatttcc
     1861 acgcggacgc catgccgcaa caacgctcgg taaccgccac agcgggccgg cggtctgctc
     1921 tactcggccg cggtatgata accgttaacg acccgggcgg gcggtaacgc tggctc
//
LOCUS       DASZMC010000523         6307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4619182,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000523 DASZMC010000000
VERSION     DASZMC010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4619182"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..352)
                     /locus_tag="VG274_10710"
     tRNA            complement(278..352)
                     /locus_tag="VG274_10710"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Glu,seq:ttc)
     gene            complement(402..2153)
                     /gene="recJ"
                     /locus_tag="VG274_10715"
     CDS             complement(402..2153)
                     /gene="recJ"
                     /locus_tag="VG274_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HWE07171.1"
                     /translation="MSVTRSFSGRKWIFRQTDRDEARNLALGADISPTLAQLLLTRGV
                     TVESVADYLRPTLKRLLPEPMTLKDMDKAVARAELAVRRGETIAIFGDYDVDGSCSAA
                     LLYEFLAALGSTPRLYVPDRLLEGYGPNAPALLRLKESGVSLVITVDCGAAAHAPLDT
                     ARDAQLDVIVLDHHAVEKAVPACAHVNPNQRGDQSGLGYLCAAGVTFLFAVALNRSLR
                     ESGRYAQTGTVEPDLFASLDLVGLATVCDVVPLVGVNRAFVRAAESRLSKLERCGIRA
                     LAAVARVEPPFSLYHCGFVFGPRINAGGRVGRASLGAELLTARSDQDAAPLAELLDRH
                     NRERQAIETLIIEEAIGLAAMQDNSPFILVAGESWHPGVVGIVAGRLKERFLKPAFVV
                     GFSDGRGRASARSVMGIDIGAIVRAARDDGLLDDGGGHAMAAGFSIAADRLDALREYL
                     GTAFASSAGSFGGVADLWLDALVSPGGATPQLATEIGRAGPFGAANAEPVLAACDVRL
                     VFADTVGKGHLRLRLQGGDGSRLSAIAFRAAGTPLGENLLRARGERIHVAGFLRENRW
                     NGRTEAQLQLEDAAAAA"
     gene            complement(2182..3141)
                     /gene="glpX"
                     /locus_tag="VG274_10720"
     CDS             complement(2182..3141)
                     /gene="glpX"
                     /locus_tag="VG274_10720"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HWE07172.1"
                     /translation="MPQPMDRKLALEAVRVTEAAAIAASRQIGRGDERAADEVAVEAM
                     RAALNGLAIDGTVVIGEGERDEAPMLYIGEHVGTGGVEVDIALDPLEGTTLTAKALPN
                     ALTVIAIAEKGSLLNAPDTYMDKIAIGPGYPDGVVDLDADPAHNIAALAKAKGVPASE
                     ITACILDRPRHAAIIESVRKAGGAVRLITDGDIAGVIATSDPVTGIDIYMGQGGAPEG
                     VLAAAALRCVGGQIQARLVFRNDEERSRATRLGISDFRRKYKLTDLAGGDVAFAATGV
                     TDGSMLEGVHRHGQFVTTSSILMRSGTRTVRWVKARHIRGHAP"
     gene            complement(3167..4456)
                     /locus_tag="VG274_10725"
     CDS             complement(3167..4456)
                     /locus_tag="VG274_10725"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWE07173.1"
                     /translation="MSRPLRIAVAGLGTVGGGVVRVLSERADDLAERAGRELVPALVC
                     ARDRRKRRAAGIAAIPWADDPLALAQADVDVVVELIGGENGVALGLIKASLDAGKHVV
                     TANKALLSRHGGELAAVAEQRGVGLKFEAAVAGGIPIVKALRESLVAHGVGAVRGILN
                     GTCNYILTQMESTGRSFGDVLADAQRMGFAEADSTLDVGGGDTAHKLAILASLAFGAR
                     PSVELMSITGIAHVTPDDIAFAREFGYRIKLLGVARMSAEGIDQRVQPAMVKLGTPLA
                     DVDGAFNAVVADAGEAGPFFFEGRGAGEAPTAAAVIADLMDIARGSVGPAFGRPAANL
                     TTASAAPPGARTSAFYLRFEAVDRPGVLAEIAGHLAQFDVSIESMIQRGRAPGESVAI
                     VMITHECTHIAVESALNVIAGSDKVLSAPVMIPMEAG"
     gene            complement(4467..5300)
                     /locus_tag="VG274_10730"
     CDS             complement(4467..5300)
                     /locus_tag="VG274_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07174.1"
                     /translation="MTSKTGFRSNLLARIAMPILVASGLCACSDIPDWVDPTTWIGGD
                     SQTAGDQDQASSASTDTAQTPDIASVPEKPAPPSTSDEQKQVAESLEADRTRAHYSTE
                     ALRGGTEAAAPPPSAEPAPSPADQGASNAQPQPSADTGQAADNAAPAASESAGPDQNA
                     AATPPPAPAPQPAAAPPPAPATSAGRMASADTASTSEPVAAPMPAATATASMQASFAP
                     SSAPPLDPSVAQFVPQPILLRYRQTAAAASAPGVAEQASAIRATHRHRKKAKPVAAAQ
                     P"
     gene            5579..>6307
                     /locus_tag="VG274_10735"
     CDS             5579..>6307
                     /locus_tag="VG274_10735"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07175.1"
                     /translation="MSKHAEPRKPAKRPPHGPDSRTNAAAAATGAPAEDAYRITDPGE
                     FARNMVRVGIQSQRILGDFLKNQTAKFGSEPLDPLNLASTFMDLLRGMVADPSAIMEA
                     QFALWRDYMGLWERTARRMMGGEAQPSVLPLPGDKRFKDRDWQENQIFDFIKQSYLLT
                     ANWLQNTVARVEGLDPQSRRRIEFYTRQFADAFAPTNFVLTNPEVLRATLKSNGENLV
                     RGLNNLLEDLDRGQGQLSIRQTTDA"
BASE COUNT         1143 a         2100 c         2023 g         1041 t
ORIGIN      
        1 cgacacgaag cggactcacg gagcattgtc ccaatccctg tcaaccggac cgggctccag
       61 ttacgcgggg ccatggacgc ccataacggg aggggttccc gtcgcacgcg tccgggagag
      121 cgccggggcg tcggaactga atcttcctcc gcagtcgcag ggtgccggcg acgtctccat
      181 acctcccgtg gtcgttccaa caaggcgtgg cggccatgaa ggcaacattc cgagatatcc
      241 gccttgacgt gcccgcgttt acaacagcca acagacatgg cgcgccctag gggagtcgaa
      301 cccctcttgc aagattgaaa atcttgagtc ctaaccgata gacgaagggc gcgggatcgg
      361 gcggctataa agacagcgcg cagggttggc aagatacggg ttcaggcagc cgcggcggca
      421 tcttcgagct gaagctgagc ttcggtccgg ccgttccacc ggttctcgcg caagaatccc
      481 gcaacatgaa tgcgttcgcc gcgcgcgcgc aggagatttt cgccgagcgg cgttcccgcg
      541 gcgcggaagg cgattgccga aagccttgaa ccgtcgccgc cttgcaggcg tagccgcaga
      601 tggcccttcc caacggtgtc ggcaaagaca agccgcacgt cgcaggccgc aaggaccggt
      661 tcggcattcg cggcgccgaa cggacccgcg cgtccgattt ccgttgccag ttgtggcgtc
      721 gcgcccccgg gcgaaacgag cgcgtcgagc cacaaatccg ccactccgcc gaacgaaccc
      781 gctgagctcg cgaaagccgt gcccagatat tcccgcaacg cgtcgaggcg gtctgctgcg
      841 attgaaaagc cggcggccat cgcgtggccg ccgccgtcat cgagcaggcc gtcatcgcgg
      901 gctgcgcgga cgatcgcgcc gatgtcgatg cccatgacgg accgcgcaga ggcccgtccg
      961 cgcccgtcgc tgaatccaac cacaaaggca ggtttgagaa agcgctcctt tagccgtccg
     1021 gcgacgatcc ccaccactcc gggatgccag ctttcgcccg ccacgagaat gaacggagaa
     1081 ttgtcctgca tggcggcaag cccgattgcc tcctcgatga tcagggtttc gatcgcctgc
     1141 cgctcgcggt tgtggcgatc gagcagctcc gcaagcggcg cggcatcctg gtcgctgcgc
     1201 gccgtcagca attctgcgcc gaggctcgcc cggccgacac ggccgcccgc gttgatgcgc
     1261 ggaccgaaca cgaaaccgca atgatagagc gagaatggcg gctccacgcg cgcgaccgcc
     1321 gcaagggccc ggattccaca acgttcgagt ttcgaaagcc tcgactccgc cgcgcgcaca
     1381 aaggcccggt tgacgcccac cagcggcacc acatcgcaaa ccgtcgcgag gccgacgaga
     1441 tccaggcttg caaacaaatc cggctcgact gttcccgtct gcgcatagcg gccggactcg
     1501 cgaagactcc ggttgagagc aaccgcgaac aggaacgtca cgccggcggc gcagagatac
     1561 ccgagtccgg attggtctcc gcgttgattg ggattgacat gggcgcaggc gggaacggct
     1621 ttctccaccg cgtgatggtc gagaacgatc acgtcgagct gcgcgtcgcg cgcagtatcg
     1681 agcggcgcat gcgccgcggc gccgcaatca acggtgatga ccagtgaaac acccgattcc
     1741 ttcagcctca gcaaagcggg cgcattgggt ccgtaaccct ccagcagccg gtcgggaaca
     1801 tagagacgcg gcgtcgatcc gagagccgcc agaaattcgt aaagcaaagc ggccgaacag
     1861 gatccgtcga catcgtagtc tccgaagatc gcgatcgtct cgccgcgcct gacagccagc
     1921 tccgcccgcg caaccgcttt gtccatgtcc ttgagcgtca tcggctccgg caaaagccgc
     1981 ttgagagtcg gcctcagata gtcggcgacg ctctcgacgg tgacgccgcg cgtcaaaagc
     2041 aattgcgcca gcgtcggtga gatatcggcg cccagcgcga ggttacgggc ttcgtcccga
     2101 tcggtctggc gaaagatcca tttgcggccg gaaaaagagc gcgtgacgct caaggccgcg
     2161 gcagacgcac aaaaggcagg atcaaggcgc atgcccacga atgtggcgcg ctttgaccca
     2221 gcgcacggtt cgcgtgccgg agcgcatcag gatgctcgac gtggtcacga actggccgtg
     2281 ccgatggaca ccctccagca tcgaaccgtc ggtgacgccc gtagcggcga acgcgacatc
     2341 gccgccggca agatcggtca atttgtattt gcgcctgaaa tcggaaatgc cgaggcgcgt
     2401 tgcgcgcgaa cgttcttcgt cgttgcgaaa gacgaggcgc gcctggatct ggcctccgac
     2461 gcagcgcagc gccgcggcgg ccaggacgcc ctccggggca ccaccctgcc ccatgtatat
     2521 gtcaatgccg gttacgggat cgctggtcgc gatcacgccc gcaatgtcgc catcggtgat
     2581 caagcgcacc gcgccgccgg ccttgcgcac gctttcgatg atggcggcgt gccgcggcct
     2641 gtcgaggatg caggcggtaa tctcggatgc cggcacgccc ttcgcctttg cgagggcggc
     2701 aatattgtga gccggatcgg catcgagatc gacgacgccg tcgggatatc ccggtccgat
     2761 ggcgatcttg tccatgtagg tgtcgggcgc gttgagaagc gagcccttct ccgcgattgc
     2821 aatcaccgtg agcgcattcg gaagcgcctt ggctgtgagc gtcgttcctt ccagcggatc
     2881 gagcgcgatg tcgacttcga cgccgccggt cccgacatgc tctccgatgt acagcatcgg
     2941 cgcttcgtcg cgctcgccct cgccgatgac caccgttcca tcgatcgcca gaccattgag
     3001 cgccgcgcgc atggcctcga ctgccacctc gtcggcggcg cgctcgtcgc cgcgcccgat
     3061 ctgacgcgag gcggcgatcg ccgcagcctc ggtcacccgg acagcttcga gcgccagttt
     3121 gcgatccatc ggctgtggca tttgtctctc cggatgccgg cggaattcag cccgcttcca
     3181 tgggaatcat caccggtgca ctcaatacct tgtccgagcc ggcgatgacg ttcaaggcgg
     3241 actcgaccgc gatgtgcgtg cattcatggg tgatcatgac gatcgcgacg gattctccgg
     3301 gcgcgcgtcc gcgctggatc atgctttcga tggaaacatc gaattgcgcc agatggcccg
     3361 cgatctccgc cagcacgcct ggcctgtcga ccgcctcgaa ccggagatag aaggcgctgg
     3421 ttctggcgcc gggcggcgca gcacttgcgg ttgtcagatt ggcggccggc cgtccgaaag
     3481 ccgggccgac cgagccgcgc gcgatgtcca tcagatcggc gatcaccgcc gccgccgtgg
     3541 gcgcttcgcc ggcgccccgg ccctcgaaga aaaacggccc cgcttccccc gcatccgcga
     3601 caacggcgtt gaaggcgccg tcgacgtcgg cgagcggcgt tcccagcttg accatagccg
     3661 gctgcacgcg ctgatcgatt ccctccgcgc tcatgcgcgc gacgccaagc agcttgatgc
     3721 ggtagccgaa ttcgcgcgcg aaggcgatgt cgtcgggcgt aacgtgcgcg atgcccgtga
     3781 tcgacatcag ttcgacactg ggtctggcgc caaaagcgag gcttgcgagg atggcgagct
     3841 tgtgcgcggt gtcgccgcct ccgacgtcca gagtcgaatc ggcctccgcg aagcccatcc
     3901 gttgtgcgtc ggcgagaacg tcgccgaagc ttcgccccgt cgattccatc tgcgtcagaa
     3961 tgtagttgca ggtgccgttc agaatcccgc gaacggcgcc gaccccatgc gccaccaggc
     4021 tctcgcgcaa agccttgacg atgggaattc cgccggccac cgctgcttcg aatttgagtc
     4081 cgacgccccg ctgttccgcg actgcggcaa gctccccgcc gtgccgcgac aacaaagcct
     4141 tgttggccgt gacgacatgt ttgccggcat cgagagaagc cttgatgaga cccagcgcga
     4201 cgccgttctc gccgccgatc aattcgacga ctacgtccac atccgcttgc gcaagcgcca
     4261 gcgggtcgtc ggcccacggg attgccgcaa taccggcggc acgccgcttg cgccgatcgc
     4321 gcgcgcaaac cagcgccggc accaattcac ggccggcgcg ctcggcaaga tcgtccgcgc
     4381 gttcggaaag aacacggacg acccctccgc cgacagtgcc gagtccggcg acggcgatcc
     4441 gcaacggcct gctcatgccg attatctcag ggctgcgcgg cagctacggg ttttgccttc
     4501 ttgcgatggc ggtgcgtggc tctgatggca gaagcctgct ctgcgacacc cggagcggac
     4561 gccgctgccg ccgtttgccg atagcgcaac aggatcggct ggggcacgaa ctgcgcaacc
     4621 gagggatcga gcggcggggc actggacggc gcaaagctcg cctgcatgct tgcggtggct
     4681 gtcgcggcgg gcattggagc agcaaccggc tcagacgtgc tggccgtgtc ggcgctcgcc
     4741 atccggccag ccgatgtcgc cggtgcgggg ggcggcgcag cagcgggttg tggcgccggg
     4801 gccggcggag gagtagccgc ggcgttctga tccggacccg ccgattcgct tgcggccggg
     4861 gcagcgttgt ccgctgcctg accggtgtcg gcggacggtt ggggctgcgc attcgatgcg
     4921 ccctgatcgg caggggatgg cgccggctcg gcggacggag gcggagccgc ggcctcggtg
     4981 cctccgcgca aagcctctgt gctgtaatgc gccctggtgc ggtcggcttc gagcgattcg
     5041 gcgacctgct tctgctcgtc gctcgtggac ggcggcgcgg gtttctcagg gacgctcgcg
     5101 atgtccgggg tttgcgcggt atccgtgctg gcgctggagg cctggtcctg atcgcccgcg
     5161 gtctggctgt cgccgccgat ccacgtcgtg ggatcgaccc aatcgggaat atccgagcac
     5221 gcgcacagtc ccgaagcgac cagaatcggc atggcgatgc gcgcaagcaa attggagcga
     5281 aagccagttt tcgatgtcat gatgtatctc cgatgagagc gacgcccgat gctccgcacc
     5341 gcgccagggc aaccaaatcc tatactaggc caaaacacgc cgtccaagtg aatccgtgac
     5401 agacgcccga cgcaggccca catggttcga caagctcacc atcaggacaa atcctccatc
     5461 ctgagcctgt cgaaggatgc ggctcgaaat cccgccatgt tgttgggatt gcagggttcg
     5521 cgccttgcgg gtgcaagcag ccgcagagta tcaagcttgc gacacggaaa ggcatttgat
     5581 gagcaagcac gcagagccca gaaaacccgc gaaacgcccg ccgcatggac cagacagccg
     5641 cacgaacgcg gccgcggcgg cgaccggcgc gcccgccgaa gacgcgtacc ggatcacgga
     5701 tccgggcgag tttgcccgca acatggtgcg tgtcggcatt cagtcccagc gtattctcgg
     5761 ggactttctc aagaaccaga ccgcaaaatt cggcagcgaa ccgctcgatc cgctcaatct
     5821 cgccagcact ttcatggatc tcctgcgcgg catggtcgcc gatccgtccg ccatcatgga
     5881 agcgcagttc gcgctctggc gggactatat ggggctttgg gaacgcacgg cgcgccgcat
     5941 gatgggcggc gaagcccaac cttcggtgtt gccgttgccc ggcgacaaac gattcaagga
     6001 ccgcgactgg caggaaaacc agatcttcga ctttatcaag caatcctatc ttctgaccgc
     6061 caactggctt cagaatacgg tcgcgcgcgt cgagggactc gatccgcagt cccgccggcg
     6121 catcgagttt tacaccaggc agtttgcgga cgcgttcgcg ccgacgaact tcgtgctcac
     6181 aaacccagag gtgctccggg cgacgctcaa gtccaatggc gagaatctcg tccgcggact
     6241 gaacaatttg ctggaggatc tcgaccgcgg ccagggccag ctttcgatcc gccaaaccac
     6301 cgacgcc
//
LOCUS       DASZMC010000524         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5162753,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000524 DASZMC010000000
VERSION     DASZMC010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5162753"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..2293)
                     /locus_tag="VG274_10740"
     CDS             complement(407..2293)
                     /locus_tag="VG274_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE07176.1"
                     /translation="MTDEEEETPEPVAPAPPAANAGMDSPADPATRDLLAALTAEQIG
                     LAIMQRQHLHMQHIRDRFLLAFDLSLAVGGVVIVAIIVALFWDAWTSRSVIVDPFDVP
                     ASFAAQGVSGRVVASELLDRLKAFQDATRTNQLKRAVADAWSNKIELRIPEAGISIDE
                     VENLLHRWLSRDEHISGSVVEDGSGIALTIRGDRFSARTFTGKRQQLHALTIKAAEYV
                     YGGSEPYLFGVYLEEQGRAPEVIVLAQSAFSMASRSDKPLLLSVWSNALADLGRYREA
                     RDKAREAVRLDPRFWLGYDNMMGDEQSLGEEEEIVDTGRDMERLARRGSWLAADVPAG
                     YWQNLDNSVADWPAFHRDVVFDMAQNGGQGTENAAEAPIDAEALARMHDWRGAELELE
                     TSPGTNTDHYVIAQSAFVRAIIALDEGDFAHAAALMRTADKIASTDSSVASNLAAPWA
                     CWLARAEALSGDSKKVDADIARGGRFVDCYRFKGDIADRRGDWPAAQKDYAAAVALAP
                     SIPSSYESWGEGLARHGHADAAFAEFALAHEKGPHWADPLEHWGEVLAGEGRFEDAIA
                     KYKDASAYAPDWGSLYLDWGRALDRLQRHDEAREKFHRAWSLDLAPHERMSIAGCCGR
                     QAPQ"
     gene            2503..>2874
                     /locus_tag="VG274_10745"
     CDS             2503..>2874
                     /locus_tag="VG274_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07177.1"
                     /translation="MKLNLTNGFIGAAALVAVGAIAGHFIHVGPFTGQPYPPDSGCNG
                     VAWNSNSSAANSYKIVFATPSGSSITVMVDFTWDYTPGYHHNVAMPAGNTFDIDQDAC
                     TNSNNVLTLNLHPQSSNPHLAD"
BASE COUNT          522 a          974 c          817 g          561 t
ORIGIN      
        1 tagcgtcatc gccggcgatt cgatcaatac atacactgtc cccagatcac tccccctccg
       61 ccgcctagct gatatctttc ctctgccatt tactttcctc ccccgcgctt cgcgcggggg
      121 aggaacctca cgcccgctgc ccccgcatcc cgatggcgta gtccggcagc ggttcccgat
      181 cgcggatgtt tgtttccacg ctcgcccttc gacaggctca gggcgaggaa cttcaccaag
      241 gccctcatgc tgagcctgtc gaagcatgag cagattctcc gccttcgccg ccttgcgtgc
      301 aacttcggcg cgttccgccg cggcgagtct ttcaaaccac tggcgcagcg cccgtttgtt
      361 gggtccctct tcgggatgcg gatttacaat accctgcgtt ttcccctcac tgaggcgcct
      421 gccttccgca acagccggcg atcgacatac gctcgtgggg cgcaaggtcg agcgaccacg
      481 cccggtggaa tttttcgcgc gcctcatcgt gacgctgaag gcgatcgagc gcgcggcccc
      541 aatcgagata gagcgatccc caatcgggcg cgtaggccga cgcgtccttg tatttcgcaa
      601 tagcgtcttc aaacctgccc tcgcccgcca gcacctcgcc ccaatgttcg agcggatcgg
      661 cccagtgcgg ccccttctcg tgggcgagcg cgaattcggc gaatgccgca tcggcgtgtc
      721 cgtgccgcgc gagcccttcg ccccagctct cataggacga cggaatcgaa ggcgcgagcg
      781 ccaccgccgc cgcataatcc ttttgcgccg ccggccagtc gccgcgccgg tccgcgatgt
      841 cgcccttgaa gcggtagcaa tcgacgaagc gtccgccgcg cgcgatatcg gcgtccactt
      901 ttttgctatc gccggagagc gcttcggcgc gcgccagcca gcacgcccat ggcgccgcga
      961 gattcgaggc gaccgaactg tccgtcgatg cgatcttgtc cgcggtccgc atcagggccg
     1021 ccgcatgcgc aaaatccccc tcgtccaacg caatgatcgc gcgcacgaac gcgctttgcg
     1081 cgatgacgta gtggtcggtg ttcgtgccgg gcgaggtttc gagttccagt tccgcgccgc
     1141 gccaatcgtg catccgcgcc agcgcctcgg cgtcgatcgg cgcctccgcg gcgttctcgg
     1201 ttccctggcc gccgttctgc gccatgtcga acacgacgtc gcgatgaaac gccggccagt
     1261 ccgcgacaga gttatctaaa ttctgccagt aacccgcagg gacgtcggct gcgagccagc
     1321 ttccgcgccg ggccagccgc tccatgtccc gtccagtatc cacgatttcc tcctcttcgc
     1381 cgagcgactg ttcgtcgccc atcatgttgt cgtagccaag ccagaagcgc ggatcgagac
     1441 ggacggcttc gcgcgccttg tcccgcgctt cccggtagcg cccgagatcg gccagcgcat
     1501 tgctccagac actgagcaag agcggtttgt cgcttcggct tgccatcgaa aaagcacttt
     1561 gcgcgagcac aatcacttcc ggagcccgcc cctgctcttc gagatagacg ccgaagagat
     1621 agggctcgct gccgccatag acgtactccg cagccttgat cgtcagcgcg tgcaattgct
     1681 gccttttccc cgtgaatgtt ctggcggaaa aacgatctcc cctgatggtc aaggcgatcc
     1741 ccgatccgtc ctcgaccacg ctgccggaaa tgtgctcgtc cctcgacagc cagcgatgca
     1801 acaggttttc cacttcgtcg atcgagatac ccgcttccgg aatccgcagc tcgatcttgt
     1861 tgctccaggc atccgcgacg gcgcgcttga gctggttggt gcgcgttgca tcctgaaatg
     1921 ccttcaggcg gtcgagcagc tcggaagcaa ccacccttcc cgaaacgccc tgcgccgcga
     1981 agctcgcagg cacgtcgaaa ggatcgacga tgaccgagcg cgacgtccag gcgtcccaga
     2041 acaaagccac gatgatcgca acgataacca cacctccgac ggcaagcgaa agatcgaagg
     2101 caagaagaaa ccggtcgcga atgtgctgca tatgcagatg ctggcgctgc atgatcgcca
     2161 gcccgatctg ctcggccgtc agcgcagcca ggagatcgcg cgttgcggga tcggccggag
     2221 aatccatgcc ggcattggcg gcgggcggtg cgggcgccac cggttccggc gtttcttctt
     2281 cctcgtctgt catcggcgcc cccaaccctg tgggcaaccc catgatcatg gcttgcaaag
     2341 aaagacgtca agctaatttg tgacggattg gggccagtct atcacatccc tgccccattg
     2401 aaccgcctcg cttgttgaag agggtgagaa aacctatagt cgcggcggtc cgcttcgcgt
     2461 ctgtctgaag cgcgggcagc ggcggacaag ggggatgcaa ctatgaagct taatctcacg
     2521 aatggattca tcggcgccgc tgcactggtt gcggtgggcg ccatagccgg acatttcatt
     2581 cacgtcggac cctttacggg ccaaccctac ccgcccgatt ccggctgcaa cggcgtggcg
     2641 tggaacagta atagtagcgc cgcgaacagc tacaaaattg tgttcgccac accgagtggc
     2701 tcttcaatca cggtgatggt tgacttcacg tgggactaca cgcccggtta ccatcacaac
     2761 gtcgctatgc cggcgggtaa cacattcgac atcgatcagg atgcctgtac gaattcaaac
     2821 aatgttttga cgttgaatct gcatccccag tcttcgaacc cacacctcgc ggat
//
LOCUS       DASZMC010000525         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1618062,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000525 DASZMC010000000
VERSION     DASZMC010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618062"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VG274_10750"
     CDS             complement(<1..318)
                     /locus_tag="VG274_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07178.1"
                     /translation="MKKHIMPGSLAAAAVLLATAALAAAEKTSGGAGNSPNGSGEAIL
                     VAQIALLLLIGRGLGEIMQRLGQPTVIGQLLAGLILGPSLFGWVWPGAHDVIFPESAT
                     QKSL"
     gene            complement(315..2279)
                     /locus_tag="VG274_10755"
     CDS             complement(315..2279)
                     /locus_tag="VG274_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWE07179.1"
                     /translation="MPQTAMPATNKDGPVAAAPDAQLRFAASAVIAAIVVGAIYFGRP
                     ILVPIALAILLAFALAPIVSLLRRLRAGRVPSVIFATLAAIAIIGALVLFIGSQLARL
                     AADLPRYQHTIEDKIHAVETSASSNGVIARTASLLKNLDNQIAPSPTTGKFVAAKGTL
                     HARSPEGREVPVPVEIRAPAATPVEIIGNIFRPLIDPLATAGIAIIFVVFILLQKEDL
                     RDRFIWMAGSSDLQRAKTALDDGADRLSRFLLTQTAVNAVFGIFVGAGLWVIGVPLPW
                     LWGLVTSMFRFVPYVGVPLAAAMPIILSLAADPGWSMMLWTAGLYLILEPVTGQLVEP
                     FVYGHSVGLSPVAVVVAATFWTWVWGPVGLLLSTPLTLCVAVLGRHVERLKFLDVLLG
                     NRPPLAEEESFYLLTLAADPDEAARLAEPFLRQHSLCEYYDVALKALVLAQVDANRGA
                     LDGERSASIKATIEGLIENLSDHEERGTGEAALEHNGTGSDSVFGRELLPKDWSGQPV
                     LCVAGRSVLDEAAALLLVHLLEKRGIGARVISSTEASPARIQSLDPAHVRFICLSYLD
                     AGSGTNAHYLMRRLRRRIPDAHAMTAFWGFAGDDSRYLNAVAATGCEVVTSLKEAVAR
                     IVASVGKPPTSETPEVSDQETARARVAATA"
BASE COUNT          450 a          730 c          791 g          375 t
ORIGIN      
        1 gagactcttc tgggttgcgc tctccgggaa aatcacgtca tgcgcaccgg gccacaccca
       61 gccgaacagg gacggcccga gaatgagacc ggcgagcaat tgcccgatca cggtcggctg
      121 acccagtcgc tgcatgatct cgccgagtcc gcgcccgatc agcagcagca gggcgatctg
      181 ggcgacaagg atcgcttcgc cgcttccgtt tggcgaattg ccggcgccgc cggatgtttt
      241 ttccgccgca gccagcgcgg cggtcgcgag caggaccgcc gcggctgcga gcgaacctgg
      301 catgatgtgc ttcttcacgc ggtggcggcg acgcgcgcgc gcgcggtttc ctggtccgaa
      361 acctccggtg tttcggacgt cggcggcttt ccgacagacg ccacgatgcg cgcgaccgct
      421 tccttgaggc tggtgacgac ctcgcatccc gtcgccgcga ccgcattgag atagcggctg
      481 tcgtcgcccg cgaaccccca aaacgccgtc atcgcgtgtg cgtcgggaat ccggcggcga
      541 agccgtcgca tcagatagtg cgcgttcgtg ccgctgcccg cgtcgagata agagagacag
      601 atgaaccgga cgtgcgccgg atcgagactc tggatccgtg cgggcgacgc ttcggtggag
      661 gatatgacgc gcgcgccaat gccacgcttt tcgaggagat ggacgaggag cagcgctgcg
      721 gcctcgtcga gcacgctgcg gcctgccacg caaagaacgg gctgccccga ccagtctttg
      781 ggcagcaatt cccggccgaa aacgctgtcc gaacctgttc cgttgtgttc cagggccgct
      841 tcgccggttc cgcgctcttc atgatccgaa agattctcga tcagcccttc gatcgtcgcc
      901 ttgatgctcg cgctgcgttc gccgtcgagc gcgccccgat tcgcgtcgac ctgcgcgagg
      961 acgagggcct tgagcgcgac gtcgtagtac tcgcagagcg aatgctgtct gaggaacggt
     1021 tcggcgaggc gcgccgcctc gtccggatcc gcagcgagcg tcagcagata gaagctttcc
     1081 tcctccgcga gcggcgggcg attgccgagc aggacatcga gaaatttgag gcgctcgacg
     1141 tggcggccga gcacggcgac gcagagcgtc aatggagtcg acaacaaaag gccgaccggt
     1201 ccccaaaccc acgtccagaa agttgctgca acgaccaccg cgacgggaga aaggccgacg
     1261 ctgtgcccgt aaacgaaagg ctcgacgagc tgtccggtaa ccggttcgag aatgaggtag
     1321 aggcccgcag tccagagcat catcgaccat ccgggatcgg ctgccaggga caggatgatt
     1381 ggcatcgctg cggcgagcgg aacgccgaca tagggaacga agcgaaacat cgacgtgaca
     1441 agcccccaca gccacggaag cggcacgccg atcacccaca accccgcgcc gacgaatatg
     1501 ccgaaaacgg cgttcacggc ggtttgcgtc aggagaaacc ggctgagccg gtcggcgccg
     1561 tcgtcaagcg ccgtcttggc gcgctgcaga tcgctggagc cggccatcca gatgaaccgg
     1621 tcgcgcaaat cctccttctg caaaaggatg aagacgacga aaatgatcgc gatgcccgcc
     1681 gtcgccaggg ggtcgatgag cgggcggaag atattgccta tgatctcgac cggcgttgct
     1741 gcgggcgcac ggatctccac tggcacgggc acctctcttc cctcgggcga gcgcgcgtgg
     1801 agcgttcctt ttgccgcaac gaacttgccg gtggttggcg aaggagcaat ctgattgtcg
     1861 aggtttttga gcagggacgc ggtgcgcgcg atcacgccgt tcgaagatgc agacgtctct
     1921 acggcatgga tcttgtcttc gatcgtgtgc tggtagcggg gaaggtccgc cgccagacgg
     1981 gcaagctgcg acccgatgaa caggacaagc gcaccaatga tcgcgatcgc ggcgagtgtc
     2041 gcgaagatga cagagggcac gcggcccgca cgaaggcggc gcaacagcga gacgatgggt
     2101 gcgagagcga aagcgagcag gatcgcgagc gcgataggca cgaggattgg ccggccgaaa
     2161 tagatcgcgc ccaccacgat cgccgcaatg accgcggatg ccgcgaatcg cagctgtgca
     2221 tcgggcgccg cggcgacggg tccgtccttg ttcgttgcgg gcatggcggt ctgaggcatc
     2281 ggccccgtcc ctcggcaagg caaaggatgg gaacgtttcc ggggttatta agttcgcctg
     2341 ggcggg
//
LOCUS       DASZMC010000526         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2693076,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000526 DASZMC010000000
VERSION     DASZMC010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693076"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..850)
                     /locus_tag="VG274_10760"
     CDS             complement(8..850)
                     /locus_tag="VG274_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07180.1"
                     /translation="MSGGRFDGWIGRTESVADVAVPGPLERLSALLDHKNPPWPQREV
                     PPLAHWLYFLPCAPQSQIGPDGHPRRGDFLPPIDPPRRMWAGSRVSFLRPLPIGAAVL
                     RRSAIASVTEKSRGGGPMTLVTIRHEISAEGTLAIVEEQDIVYRNVGSPASRPSREAG
                     QPMRAAASREIVLDSIALFRFSALTFNAHRIHYDREYARNVEGYPGLVVQGPFTATLL
                     MDHFLGTNAVARIGTVAIRALRPLFADAPFQLCLAQDGDRVKLWALDPSGREAMAAHL
                     TVEN"
     gene            complement(860..>1915)
                     /locus_tag="VG274_10765"
     CDS             complement(860..>1915)
                     /locus_tag="VG274_10765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWE07181.1"
                     /translation="TYPKEFVQALTREGFLSVLIPEGFGGAGLGLGAAIATLEEIHHS
                     GANGAAAHAQMYTMGTLLRHGSEEQKRRWLPQLTSGALRLQAFGVTESGSGTDTTRIR
                     TFARRDGDTYVVSGSKIWISRAEHSDLMLLLVRTSARQEPNAGEGMSVLLVDLRDAVG
                     RGLSIRPIRTMINHATTELFFDDLSVPSENLIGREGQGFRCILDGLNAERILIASECI
                     GDGRFFVDRATAYAKDRKVFGRPIGENQGVQFPIARAHVQIEAAAEMVRKAAALFDAG
                     KPCGAEANMAKLLAAEASWSAADMCLQTHGGIGFAEEMDIERKFRETRLYQVAPISTN
                     LILSHVATHVLGLPKSF"
BASE COUNT          352 a          615 c          602 g          346 t
ORIGIN      
        1 gcggtgttca attctctacg gtcaggtgcg ccgccatcgc ttcgcggccg gagggatcga
       61 gcgcccataa tttcacgcgg tctccgtctt gcgcaaggca gagctggaac ggcgcgtctg
      121 caaaaagcgg ccgtaacgct cgaatggcga cggtaccgat acgcgcgacg gcatttgtcc
      181 ccagaaaatg atccatcagc agggtcgctg tgaatggccc ctggacgaca aggccgggat
      241 agccctccac gttccgcgcg tattcgcggt cgtaatgtat ccggtgcgca ttgaatgtga
      301 gcgcagagaa gcgaaacaga gcgatcgaat cgagcacgat ttcgcgcgag gcggctgctc
      361 gcatgggctg ccccgcctcg cgcgatggcc tggacgccgg cgagccgaca ttgcggtaga
      421 cgatgtcctg ctcttcgacg attgcgaggg ttccctccgc cgatatttcg tgccggatag
      481 tgacaagcgt cattggcccg cccccacggc tcttttccgt tacgcttgcg attgccgaac
      541 ggcgcaggac cgccgcaccg ataggaaggg gccgaagaaa actgacgcgg cttccagccc
      601 acatgcgccg gggcggatcg atcggcggca ggaaatctcc gcgtcgcgga tgcccgtccg
      661 gaccgatctg cgattgaggc gcgcaaggaa gaaaatacag ccaatgggcg agcggcggca
      721 cttcgcgttg cggccagggc ggatttttgt gatccagcaa tgcggagagg cgttcgagcg
      781 gccccggcac cgccacgtcc gccacggact cggtgcggcc gatccagccg tcgaatcttc
      841 ctccgctcat cacaggagct caaaacgact tcggcaggcc caatacatgc gtcgccacat
      901 gcgaaaggat gaggttggtg gagatgggcg ccacctgata gaggcgcgtc tcgcggaact
      961 tgcgctcgat gtccatctct tccgcgaagc cgatgccccc atgcgtctgc aggcacatgt
     1021 ccgcggccga ccaggacgct tccgcggcca gcagcttcgc catgttcgcc tccgcgccgc
     1081 agggtttgcc ggcatcgaac agcgctgcag ccttgcgcac catttccgcc gccgcttcga
     1141 tctgcacatg ggcgcgggcg atcggaaact gaacgccctg attctcgccg atcggccggc
     1201 cgaacacctt gcgatccttc gcgtaggccg tcgcgcgatc gacgaagaag cgtccgtcgc
     1261 cgatacattc ggaggcaatg aggatccgtt ccgcgttcaa tccgtcgaga atgcagcgga
     1321 acccctgccc ttccctgccg atgagattct ccgacggcac gctcagatcg tcgaagaaga
     1381 gctcggttgt cgcatgattg atcatggtgc gtatggggcg gatggaaagc ccgcgcccga
     1441 ccgcatcgcg cagatcgacg agaagcacgc tcatcccctc gccggcgttt ggttcctgtc
     1501 gtgcgctcgt tcgcacaagc agcagcatca gatcggaatg ctccgcgcgg ctgatccaga
     1561 ttttgctgcc gctcacgaca tacgtgtcgc cgtcgcgccg cgcaaaggtg cgaatgcgcg
     1621 tcgtgtcggt gccgctcccg gattcggtga caccgaaagc ctgcaggcgc agcgcgcccg
     1681 aggtgagttg cggcagccag cgacgcttct gttcttcgct gccgtgacgc aacagcgtgc
     1741 ccatcgtgta catctgggca tgagcggcgg caccattcgc gcccgagtga tggatttctt
     1801 cgagagttgc gatcgcggcg ccaagtccga ggccggcgcc gccaaagcct tccgggatca
     1861 gcacgctcag aaagccttcc cgcgtcaatg cctgcacgaa ctctttggga taggt
//
LOCUS       DASZMC010000527         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5163472,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000527 DASZMC010000000
VERSION     DASZMC010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5163472"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VG274_10770"
     CDS             complement(<1..940)
                     /locus_tag="VG274_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWE07182.1"
                     /translation="MAHIAELLQVAKLRDPAPHHNSLKSRSILGGLALAGMAVVGVSW
                     HHAGATPDPEMAANAAIPVTVAAVEQRPVRLSSEFSGRMTAVDSADVRPEVAGRITEI
                     RFKDGQTVQAGDVLFVIDPRPYEAAVAKAQADLATANTNARLAHTELDRAKKLMEAEA
                     LARDTYDQRANTVGVTQASIQSAQAVLAQANLDLDHAYVKAPISGRVGRAEITVGNLV
                     QTQSAPLLTSIVSNDGIYADFEVDEQTYMRTVRAHAATLAKEQKLPVQLTAQGDTAHT
                     YDGVIESFDNKIATGSGTIRARARFANADGALMPGMF"
     gene            1103..1678
                     /locus_tag="VG274_10775"
     CDS             1103..1678
                     /locus_tag="VG274_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWE07183.1"
                     /translation="MTVFWKNGYEGASLSDLTTAMGINAPSLYAAFGSKEGLFKAVLE
                     RYDERRSGFMEKVLSAPDAFGVARNYLEGIAEFAADTNGGANPPGCLLAQSGSCSDAA
                     IPEEVARHRAEKEQALRRRFEQAKKDGDLPKSADPTTLARYLSAMANGICMQAVAGAA
                     TAELLEIARMALASWPVQEKTKPKKKSHVAA"
     gene            1737..>1974
                     /locus_tag="VG274_10780"
     CDS             1737..>1974
                     /locus_tag="VG274_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWE07184.1"
                     /translation="MPHTTVYTIIAQSIAILFAIGGAFQLVGPAFVQRAYERWEMPRK
                     FYRVTGAIELTTALFLVNAETRVWGIVLGAIVTFS"
BASE COUNT          406 a          577 c          605 g          386 t
ORIGIN      
        1 cgaacatgcc gggcatcagc gcgccatcgg catttgcaaa acgggcacgc gcacggatcg
       61 tgccgctgcc ggtcgcgatc ttgttgtcga agctttcgat cacgccgtca taagtatgtg
      121 cggtgtcgcc ctgcgccgtc agctggacag gcagcttctg ctccttggcc agcgtcgcgg
      181 catgcgcacg cacggtgcgc atataggtct gctcgtcgac ctcgaaatcg gcatagatgc
      241 catcgttcga gacgatagaa gtgagcaacg gcgcggactg cgtctgcacc agattgccga
      301 cagtgatttc cgcccggccg acgcggccag agatgggcgc cttcacataa gcgtggtcga
      361 gatcgaggtt cgcctgcgcc agaaccgcct gcgcggactg gatcgatgcc tgtgtcacac
      421 cgaccgtatt ggcgcgctga tcataggtgt cgcgcgccag cgcctcggct tccatcagtt
      481 ttttggcacg atcgagttcg gtgtgtgcga gacgcgcatt ggtattcgcc gtggcgagat
      541 cggcctgcgc tttggcaacc gccgcttcat agggacgcgg atcgatcacg aacaacacgt
      601 cgccggcttg aacggtctgt ccgtccttga agcgaatttc ggtgatacga ccagccactt
      661 cgggccgcac atcggcggaa tccacggctg tcatgcggcc ggaaaactca gacgagaggc
      721 ggacaggcct ctgctcgacc gcggcgacgg tcacgggaat cgcggcgtta gcagccattt
      781 ccgggtccgg cgtggcgccg gcatgatgcc aggacacgcc gacgacggcc atgccagcca
      841 acgcaagccc acccagaata gagcggcttt tcaaagagtt atgatggggc gcgggatccc
      901 gaagtttggc gacctgaaga agttctgcaa tatgggccat ggaggcctcc tatttctgta
      961 ttgaacgata cagatatagg acctagacga accgcgtcaa gagcattatg tatcactcga
     1021 tacaataata aaagggaaaa cgctctcgat ggccatgggc cgcccaagaa gtttttgtgt
     1081 cgatcaggcc gctgatcgcg cgatgacggt cttttggaag aacggctatg aaggcgcgtc
     1141 tctgtccgat ctgacgacag cgatgggcat caacgcaccg tctttatatg cggcgttcgg
     1201 cagcaaggaa ggtctcttca aggccgtgct cgaacgctat gacgagcggc gcagcggatt
     1261 catggagaaa gtgctctccg cacccgatgc gttcggcgtg gcgcggaact accttgaagg
     1321 tatcgcggaa ttcgcagccg acaccaatgg tggcgcgaac ccgccaggct gtcttctggc
     1381 acagagcggc tcttgctcgg atgcggcgat ccccgaagaa gtcgctcgcc atcgtgccga
     1441 aaaggaacag gcactgcgca ggcgctttga gcaggcgaag aaggacggcg atctgcccaa
     1501 gagcgccgat cccactaccc tcgcccgcta cctttctgcg atggcgaatg gcatctgcat
     1561 gcaggcggtc gccggggcgg cgacggcaga actgctcgag attgcgcgca tggcgctcgc
     1621 gtcctggccg gtgcaggaaa agacaaagcc caagaaaaaa tcacacgtcg cggcttgatt
     1681 atgtattgat cgatacatat gttccggcgg tcgacttttg gccaccgagg catggaatgc
     1741 cgcacacaac cgtttacacc atcattgcac aatcgatcgc gatcctcttt gcgattgggg
     1801 gcgcgtttca gctcgtcggc cccgcgttcg tacagcgcgc ttacgagcgc tgggaaatgc
     1861 cgcgcaagtt ttatcgcgtg accggcgcaa tcgaactgac aaccgcactg tttctcgtga
     1921 acgctgaaac ccgcgtttgg ggaatcgtcc tcggcgcgat tgtcacgttt agcg
//
LOCUS       DASZMC010000528         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3250288,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000528 DASZMC010000000
VERSION     DASZMC010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3250288"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VG274_10785"
     CDS             <1..277
                     /locus_tag="VG274_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07185.1"
                     /translation="LAYGAAHRLALAALRHKGYRSENRITVFQTLVHTLGSDHADLQV
                     FLKANNVRNRAEYEGRMEIDGGLLADLIRCTKKLETAVAKLDPPPAE"
     gene            452..748
                     /locus_tag="VG274_10790"
     CDS             452..748
                     /locus_tag="VG274_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07186.1"
                     /translation="MLLASVLVAAGLAISGLSLVQIAVRNPQQMAQATPPLQSSPTPT
                     PQASPPAESKPGGDRPTTPAPEPARPDPQAQKEGAKPALPPAPAEKTAPPITQK"
     gene            1069..1809
                     /locus_tag="VG274_10795"
     CDS             1069..1809
                     /locus_tag="VG274_10795"
                     /inference="COORDINATES: protein motif:HMM:NF002614.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HWE07187.1"
                     /translation="MKIDAVRKTAYSMPLTSPSFPPGPYRFFNREYFVIAYRTDPEAL
                     AAVVPEPLEVTGPIVKYEFIRMPDSTGFGDYTETGQVIPVRLNGVEGAYTHAMYLDDN
                     APIAGGRELWGFPKKRASPKIEVESDVLVGTLHYGSVLCASATMGYKHRPVDHDTVLN
                     GMKTANFILKIVPHVDGSPRICELVRFYLEDIQLKEAWTGPAALGLFPHALADVARLP
                     VREVLSALHFKADLTLGLGSVAFDYMKK"
     gene            complement(1932..>3084)
                     /locus_tag="VG274_10800"
     CDS             complement(1932..>3084)
                     /locus_tag="VG274_10800"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWE07188.1"
                     /translation="DTSSVDIMTMCREIDALYIDTVVEPWLGFYFDKNIGSEKRSNYA
                     LRETLLAAKHKSPGGTTAVSTCGANPGMVSWFVKQALLDIARDTNTEFNEPKSREGWG
                     QLAHKLGVKGIHIAERDTQRSKRPKPMNVFVNTWSVEGFVSEGMQPAELGWGTHETWM
                     PDNGRTHPDGCGAAIYLLQPGANTRVRSWCPTPGAQYGFLVTHNESISIADYFTVRDG
                     SHVIYRPTCHYAYHPANDAVLSLHEMFGATGKMQPKWHILDENEIEDGIDELGVLLYG
                     HAKNAYWYGSQLSIEETRAIAPYQNATGMQVSSAVLAGMVWALENPTAGIVEADEMHF
                     RRCLEIQRSYLGPVKGFYTDWTPLTDRPGLFPEDIDTSDPWQFRNVLVR"
BASE COUNT          601 a          927 c          957 g          599 t
ORIGIN      
        1 cctggcgtat ggcgctgcac atcgcctagc gctcgctgca ttgcgccaca agggataccg
       61 gtccgaaaat cgcattacgg ttttccagac gctcgttcac acgctggggt cggatcatgc
      121 tgatctgcaa gtttttctga aggccaacaa cgtacgaaat cgggcagaat atgagggccg
      181 tatggaaatc gacggcggcc tcttggctga tctaatccgt tgcacaaaga aattggagac
      241 agcggtagcg aagctcgatc ctcctcctgc cgaatgactg aggcggcggt gtctggaaag
      301 gtcagccgcg gatttcatcg ctagtacggt gcccggacat ggcgccaagt tcccgggaac
      361 aaggcgtccc aacatccgtt gatagcgcga atgaattttc gcggtgaacg agatgtctcc
      421 ggaggaaaga aaacgcgcgt actggaggga tatgctgctc gcgtcggtgt tggtcgcggc
      481 ggggcttgcc atatccggtc tttcgttggt ccagatcgcc gttcgcaatc cgcaacagat
      541 ggcgcaggcg acgccgccgc tgcagtcgtc gccgacgccc acgccgcagg cgagcccgcc
      601 agccgaatcg aagcccggcg gcgatcgccc caccacgccg gcgcccgagc cggcccgccc
      661 cgatccccag gcgcaaaagg aaggcgcaaa accagcgctg ccgccggctc ccgcggaaaa
      721 gaccgcgccg ccgatcacgc agaaataact gccgtcattc tccgcgcaag cgcgtagcgc
      781 ttgtcgctgg gggcgcgaag ccttgcgaca aaattgatgc gcgcatcaat ttgtgctggg
      841 cggagcgagc ccggaatcca taaccacgaa tgggggttat ggattccccg acgcgcaatt
      901 gcgcgtctga ggtctgtcgc ctccggtcgg cgattgccga ccggaggcgc atcccggaat
      961 gacgggccta tgacaaggag atgctcattg ccgaatttac agcctcgctt gttgccgtcg
     1021 gctgccgcat agtcccgcaa aggggcgcga caacattatg aggcaagcat gaagatcgat
     1081 gccgtcagga aaaccgcgta ttcgatgccg ctgaccagcc cgtcgtttcc gccggggcct
     1141 tatcggttct tcaaccggga atattttgtc attgcctatc gcaccgatcc ggaggcgctt
     1201 gccgccgtgg tgccggagcc gctggaagtt accggaccga tcgtcaaata cgaattcatc
     1261 cggatgcctg actcgaccgg cttcggcgat tacactgaaa ccgggcaggt gattccggtt
     1321 cgcctgaacg gcgtggaggg cgcctatacc cacgcaatgt atctcgacga caacgcgccg
     1381 atcgcgggcg gtcgcgagct ctgggggttt cccaagaagc gcgcctcgcc gaaaatcgaa
     1441 gtcgagagcg acgtgctggt gggaacgctg cattacggct cggtgctctg cgcctccgcc
     1501 accatgggtt acaagcaccg gcccgttgac catgacacag tgctgaacgg catgaagacg
     1561 gcgaatttca tcctcaagat cgttccgcat gtcgacggca gccccaggat ctgcgagctg
     1621 gtgcgttttt atctcgagga cattcaattg aaggaagcct ggacgggccc cgccgcgctc
     1681 ggcctgttcc cgcatgcgct tgccgacgtg gcgcggctgc cagtgcgcga ggtgctctcg
     1741 gcgctgcatt tcaaggccga cctgacgctg gggttgggaa gtgtggcgtt tgattatatg
     1801 aaaaaatagc caaaactcgt catggccagg cttgtccctg ccatccacgt ggctggcgaa
     1861 ggaaaggaga cgtggatgcc cgcgacaagc gcgacaagcg tgggcatgac ggtgtgagag
     1921 tttacgccgc ctcagcgcac cagcacgttc ctgaactgcc agggatcgga cgtgtcgata
     1981 tcctccggaa acagtccggg ccggtcggtg agcggtgtcc aatccgtgta gaaacctttc
     2041 accgggccga gatacgaccg ctggatttcg aggcagcgtc gaaaatgcat ctcatcggcc
     2101 tcgacgatcc cggcagtcgg attttccagc gcccacacca tgccggcgag cactgctgac
     2161 gaaacttgca tgccggtggc gttctggtag ggcgcaatgg cgcgcgtctc ctcgatcgaa
     2221 agttgcgatc cgtaccaata ggcgttcttg gcatggccat agagcagcac gccgagctcg
     2281 tcgatgccgt cctcgatctc gttctcgtcc aggatatgcc atttcggctg catcttgccg
     2341 gtggcaccga acatctcgtg cagcgacagc accgcgtcat tggcggggtg ataggcgtag
     2401 tggcaggtcg ggcgatagat cacatggctg ccgtcacgca cggtgaagta atccgcgatc
     2461 gaaatcgatt cattgtgagt gacgagaaaa ccgtattgag cacccggcgt cgggcaccat
     2521 gagcgtaccc gcgtgttggc gcctggctgc agcagataaa tcgccgcacc gcagccgtcc
     2581 gggtgggtgc ggccattgtc cggcatccac gtctcgtgcg tgccccagcc aagctcggcc
     2641 ggctgcatgc cctcggacac aaagccctcc accgaccagg tgttgacgaa cacgttcatc
     2701 ggcttcggcc gcttggagcg ctgggtatcg cgttcggcga tatggattcc cttgacaccg
     2761 agcttgtggg cgagctggcc ccagccttcc cggctcttgg gctcgttgaa ttccgtattg
     2821 gtatcacgcg cgatatcgag cagcgcctgc ttgacgaacc acgacaccat gccgggattc
     2881 gcgccgcagg tggataccgc agttgtgccc cccgggcttt tgtgttttgc cgcgagtagc
     2941 gtctcgcgca gcgcgtaatt cgagcgcttt tcggagccga tattcttgtc gaagtagaag
     3001 ccgagccatg gctcgactac agtgtcgata tagagcgcgt cgatctcgcg gcacatcgtc
     3061 atgatgtcga ccgaggaggt gtcg
//
LOCUS       DASZMC010000529         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3520940,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000529 DASZMC010000000
VERSION     DASZMC010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3520940"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VG274_10805"
     CDS             complement(<1..1122)
                     /locus_tag="VG274_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWE07189.1"
                     /translation="MKSHTRTLASGGSKHRHPPRASARPHGETQLARMQAIAGLGTVE
                     IDFGTGAVSMSDEARRLLGWDAEAAPSVRGILQAVHEDDVAAMALFLSAPSHGKPATE
                     ECFFRLAIDERRGTLIYGRAGVSLSARGKPELVVVVVQDMTRLVEMERAAHSQAQFYR
                     GIFENSIWGIFQTTAVGHYLMANAALAQIYGFETVDALLAALTDIGVQLYVDPTRRDA
                     FVKQMRERGVVSGFESQIYRRDGSVIWISESCREVRSDDGKFLYYEGMVEEITERKRV
                     EDELRAAKEAAEAANRAKSEFLGTMSHELRTPLNAIIGFSEIMALQAFGPLGNPQYQT
                     YARDIEGSGRHLLSLISDILDLSRLEAGKADLHEEPVQIA"
     gene            complement(1204..1626)
                     /gene="ndk"
                     /locus_tag="VG274_10810"
     CDS             complement(1204..1626)
                     /gene="ndk"
                     /locus_tag="VG274_10810"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWE07190.1"
                     /translation="MAVERTLSIIKPDATRRNLTGKINARFEEKGLKIIAQKRLQLTK
                     AQAEAFYAVHKARPFFGELVTFMISGPVVAQVLEGENAVTANRDIMGATDPKKAADHT
                     IRKDFAESIEANSVHGSDSAENAAIEIAFFFAGIEIVP"
BASE COUNT          334 a          646 c          640 g          375 t
ORIGIN      
        1 cgcgatctgc accggctcct cgtgcaggtc cgccttgccg gcttcgaggc gggagagatc
       61 gaggatgtcg ctgatcagcg agagcagatg ccggcccgat ccctcgatgt cgcgggcata
      121 ggtctggtac tgcggattgc ccagcgggcc gaacgcctgc agcgccatga tctccgagaa
      181 gccgatgatg gcattgagcg gcgtgcgcag ctcatggctc atcgtcccga ggaattccga
      241 tttggcgcga ttggcggctt cggccgcttc cttggccgcg cgcaactcgt cctcgacgcg
      301 cttgcgctcg gtgatctcct cgaccatgcc ttcgtaatag aggaacttgc cgtcgtcgct
      361 gcgcacctcg cggcagcttt ccgagatcca gatgacgctg ccgtcgcggc gatagatctg
      421 cgattcgaag cccgaaacga caccgcgctc gcgcatctgc ttgacgaacg catcgcggcg
      481 cgtcggatcg acatagagct gcacgccgat atcggtcagc gccgcgagca gcgcatccac
      541 cgtctcgaag ccgtagatct gcgccagggc cgcattcgcc atcaggtagt ggccgacggc
      601 ggtggtctgg aaaatgcccc agatcgaatt ctcgaaaatg ccgcgataga attgcgcttg
      661 gctgtgcgcc gcgcgttcca tttcgaccag ccgcgtcatg tcctgcacga ccacgaccac
      721 cagctcgggc ttcccgcgcg cgctgagcga aacgccggcg cggccataga tcaacgtgcc
      781 gcgccgctca tcgatcgcga gacggaagaa acattcctcg gtcgccggct tgccatgcga
      841 cggcgccgag aggaacagcg ccatggccgc gacatcgtcc tcgtgcaccg cttgaaggat
      901 accgcgcacg ctcggcgccg cttccgcatc ccagccgagg agccggcgcg cctcgtccga
      961 catggacacc gcgcccgtgc cgaaatcgat ctccaccgtg cccagcccgg cgatcgcctg
     1021 catccgcgcg agttgcgtct cgccatgcgg ccgcgccgaa gcacgcgggg ggtgtcggtg
     1081 cttgctcccg ccagaggcga gcgtgcgggt atgggatttc atgccgccgt cttaccggac
     1141 ggcggctaaa atcccataaa ttcaattgct taaattttat gcagcgggta cgcagcgccc
     1201 cgatcagggg acgatttcga tcccggcgaa gaagaaggcg atctcgatcg cggcgttttc
     1261 cgccgaatcc gagccgtgca ccgagttcgc ctcgatcgat tcggcgaaat ccttgcggat
     1321 cgtgtgatcg gcggcctttt tcgggtcggt ggcgcccatg atgtcacggt tggcggtgac
     1381 agcgttctcg ccttccagca cctgggccac gaccgggccg gaaatcatga aggtgaccag
     1441 ttcgccgaag aacggccgcg ccttgtggac ggcatagaag gcctcggctt gggctttggt
     1501 gagctgcagg cgcttttgcg cgatgatctt gaggcccttc tcctcgaacc gagcattgat
     1561 cttgccggtg aggttccgcc gggtcgcgtc cggcttgatg atcgacaggg tgcgctcgac
     1621 ggccatgaaa agtgtccatt cagttgtgga tgggggcgcc tttggcgccc ccggaatttg
     1681 ggcaagagcg ctccagggat ttgtggatag gtgcgccccg gaattgcgcg gggtatagcg
     1741 agcgcgctgc ggtggttcaa cccacggcgg gcccacctcg ccggagttcc tattcgtttc
     1801 cccgcgaaag cggggaccca gggcaccaca gggctgcgca tccgcatcgg attcccgctt
     1861 gcgtgggaat gacggtgatg agggagcgga gacctactcg cccctcttca cccaacctga
     1921 cggcgtctgc tcccaatagg ttagcgtgtg cccgaccgcc ttggcctcgc gccaacgttg
     1981 gcgggcctct tcgac
//
LOCUS       DASZMC010000530         8209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4073551,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000530 DASZMC010000000
VERSION     DASZMC010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4073551"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VG274_10815"
     CDS             complement(<1..1151)
                     /locus_tag="VG274_10815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWE07191.1"
                     /translation="MMKLSFHGADHDVTGSCHLLEAAGRRVLVDCGLFQGGRELNEDN
                     AAPFGFDAAAIDHVLLTHAHLDHCGRLPLLGNRGFKGEIITSSASRELAQLVMLDSAH
                     LQEEDSRRQSMKLARRGASRRAATPLYTTSDATRILINFGRTAEYGQTLELGPGLRAT
                     WFDAGHILGSASILVEVDEAGTTKHVVFSGDLGHADGPLLRPPMAPPSADAVVMETTY
                     GDRLHKPRDASIEELYSAILETFARGGNVVIPTFALERAQEILFFLHQGVADNRLPPS
                     IQVYLDSPMAISATAIFARHPECLKPEAAALFHDGQDPFHVPGLHFTRETADSMAINK
                     IRNGAVIMAGAGMCTGGRVRHHLKHNLWRPEASVVFVGYAAQGTPAQQI"
     gene            complement(1148..2128)
                     /locus_tag="VG274_10820"
     CDS             complement(1148..2128)
                     /locus_tag="VG274_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWE07192.1"
                     /translation="MKKNRRAPRRSKIPQPPNPVERRTPLPSQHPKPAAEDPLAPAAL
                     KAILASPSYRQADQDTDFLARRDTRNTRLALDYEKAELLLKERGIAHTVVVFGSTRIC
                     EPAEAQRNVDVLTNELEAAPANKDVARRLDVARRILAKSKYYEIAREFGRLVSESGAK
                     RRRNRTVIVTGGGPGIMEAANRGASDAGAQSVGLNITLPREQFPNPYVTPELCFRFHY
                     FAIRKLHFLLRARALVAFPGGYGTMDELFEVLTLGQTRKQSPLPVILVGESYWRKVFD
                     PEFLVAEGVIDPEDRELFWFAESAEGIWKSILDWYDAAGRPLLGQEQGPE"
     gene            complement(2147..5533)
                     /locus_tag="VG274_10825"
     CDS             complement(2147..5533)
                     /locus_tag="VG274_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWE07193.1"
                     /translation="MKLQVWRRAALAAVIALGCTQAQPGSAHDWTFMRFPALHGGSIV
                     FEAHGNLWQVGRWGGAASRLTADPGYDLMPRYSPDGSAIAFTGFYQGNQDVYVMPAGG
                     GAAKRLTFHSDVVESAPTRWGPDNMVVTWTPDSKNVVFLSRRMAWNSWYGRLFEVPAG
                     GGLAQPLPLDRGGLMTYSPDGKSIAYNRIFRNFRTWKRYNGGLGQDVYTYAFDTKKLT
                     RITTWLGTNTAPMWVGRKIYYLSDHDAKRRENIWVYDLDSKQFREVTHFTNYDIDFPS
                     LGLGGKGGDGIVFAQAGALWVIDLPSEKLHRLNVSVPDDGTRTGPRYADASKAIRDQD
                     TAQDTDYALSPNGKRAIFSARGDLFTAPAEHGATRNLTGTSNADEDHPGWSPDGKTIA
                     YTTDVSGEQQIAVRPATGGAEKILTHFTAGFLYGPLWSPDSKRLAVADGNHRLWLVDA
                     SSGNATQVAQDPYSEIHDQSFAPDGRWLAYSKTAPNQQKGVWVDDIGSGRSVRVSEPL
                     ANDTDPVFSPDGKYLYFVSTRHENPTANEIEFNVASIEMDGIYVATLRKNEPSAFAPR
                     SDEGAFESEKDSKSKPWKPGASAPVAIDFDGLMARAVPLPIPAADIAQIDARDDNVYY
                     RIAQPQMLEGPLPRMKSAMHVYNLEKRKDATLVEDLDSYSLSYNGEKALYKKTGAGDD
                     SDDDGKGYAIIDAKMPEDDKKPDPKSLDLSHMRTRIEPVQEWAEMFNNAWRLERDLFV
                     NPKMNGVAWQAVHDAYAKLLPLVGSREDLTYLIGEMQGELGNSHTYVGGGDEDNPADT
                     VSTALLGVDFAPTPSGHYSLAKIYPGDNTREKYRSPLLEPGVDVREGDVLLAVNGRDL
                     KAPADPYSLLVGVDDQPVTLTVERAGGKPHDVTVKPIKNELSLREKDWIDHNRAVVDK
                     LSGGKIAYIYMSDMETLGMEQFIRQFYPQIDRQALILDDRWNGGGNIDQMILERLRRI
                     LVGLDTNRERASTSIPQQLISGPKVCLINNYSASDGDIFPYYFRKYGLGPLIGTRTWG
                     GVRGIRGDWTLLDGGYITVPEDSLYGLRSEWVIENHGVDPDMNIDTDPGQLLAGRDAQ
                     LEAGVQYLMRALQKHPGGLPPPPPPLPAYPPQGQIPPPSL"
     gene            6747..7010
                     /locus_tag="VG274_10830"
     CDS             6747..7010
                     /locus_tag="VG274_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWE07194.1"
                     /translation="MKQVTLREANQQFSRLVREVEETGERVLVLRDGKPAVEIAPTAA
                     RRAMRKLTREQETAFGAFVDSAVKRPGSSKGARRWSRDELHER"
     gene            7000..7455
                     /locus_tag="VG274_10835"
     CDS             7000..7455
                     /locus_tag="VG274_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWE07195.1"
                     /translation="MNAERFTLDTNILVYAVDARDRRKHALAVDILKAAVRLDCPLAL
                     QAIGEFYVAAVGKVKLAPDDARERALQLMTGFEAFAHSASAVRAGLEESARGRFSFWD
                     AVLLASAAEAGCSVILSEDMHDGARLGSIAICNPFGAKGLSPAARELFG"
     gene            complement(7834..>8209)
                     /locus_tag="VG274_10840"
     CDS             complement(7834..>8209)
                     /locus_tag="VG274_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07196.1"
                     /translation="VTGARDSFHLAMDMASMHSMRDWCVSNVEDHISAFADHDVVDSV
                     ALSRALDTLLNPAPSDPARLAGCRSAIQAELETKLRQVESFIQAGERDSARGLLEQID
                     ARFGGMAAPRSVELAARLKEKN"
BASE COUNT         1430 a         2500 c         2671 g         1608 t
ORIGIN      
        1 atgatctgtt gcgcgggcgt gccctgcgcc gcatagccga caaaaaccac gctcgcttcc
       61 ggccgccaga gattgtgctt gaggtggtgg cggacgcggc ctcccgtgca cattccggcg
      121 ccggccatga tgaccgctcc atttcgaatc ttgttgatcg ccatcgaatc cgcggtctcg
      181 cgcgtgaaat gcaatccggg aacgtgaaaa ggatcctgcc cgtcatgaaa aagcgccgca
      241 gcctccggtt tgagacattc cggatgccgg gcaaaaatgg cggtggccga aatggccatt
      301 ggcgaatcca gatagacctg gatggagggc ggcaggcgat tgtcggcaac accctgatga
      361 agaaagaaca ggatctcctg cgcccgctcg agcgcaaagg tggggatgac cacattgccg
      421 ccgcgcgcga aggtctccag gattgccgag tagagttctt cgatcgacgc gtcgcgcggc
      481 ttgtgaagac gatctccata ggtcgtttcc atgacgacag cgtctgcgga aggaggcgcc
      541 atgggcgggc gcaaaagtgg gccgtcggca tggccgagat ccccggaaaa gacaacatgc
      601 ttcgtcgtgc cagcctcgtc gacttcgacg agaatgctcg ccgaaccgag aatgtgcccc
      661 gcgtcgaacc atgtggcgcg cagaccgggt ccaagctcga gtgtttgtcc gtattccgcg
      721 gtccgcccga agttgatgag aatgcgcgtc gcgtccgacg tcgtatagag aggcgttgcg
      781 gcgcgccggg aagcgccgcg gcgggcgagc ttcatggatt gacgccgcga atcctcctcc
      841 tggagatgcg ccgaatcgag catcaccagc tgcgcgagtt cgcgcgaggc gctgctggtt
      901 atgatctcgc ccttgaagcc gcggttgccg agaagcggca gacgcccgca atgatcgaga
      961 tgcgcgtgcg tgagcagaac gtgatcgatg gcggccgcat cgaatccgaa tggcgcggcg
     1021 ttgtcttcgt tgagttctct tccgccctgg aagagtccgc agtctaccag aacgcggcgt
     1081 cccgcggctt ccagcagatg gcaggaaccg gtgacgtcgt gatccgcacc atgaaaggaa
     1141 agcttcatca ttcgggtcct tgttcctggc cgagcagcgg tcggcccgcg gcgtcgtacc
     1201 agtccagaat gctcttccag atgccctcgg ccgattccgc aaaccagaag agttcgcgat
     1261 cctcggggtc gatcacgcct tcggcaacaa ggaattccgg atcgaacact tttcgccaat
     1321 aggattcgcc cacgaggata accgggaggg ggctctgctt gcgcgtctgg ccaagggtca
     1381 gaacctcgaa aagctcgtcc atcgttccgt agccgccggg aaacgccaca agcgcccggg
     1441 cgcgcaacag gaagtgcaat ttgcgaatgg cgaaatagtg gaaacggaag cacagctcgg
     1501 gagtgacata ggggttgggg aactgctcgc gaggaagggt aatgttgagg cccacggatt
     1561 gtgcgcccgc atcgctggcg cccctgtttg ccgcttccat gattcccggt ccgccgccgg
     1621 tcacgatcac agtccggttg cgcctgcgtt tcgcgccgga ttcgctgacc aggcggccga
     1681 attcgcgcgc gatctcgtaa tatttgctct tcgcgagaat gcgcctggca acgtcgagcc
     1741 ggcgcgccac gtccttgttg gcgggcgcgg cttcgagttc gtttgtgaga acatcgacat
     1801 tgcgctgggc ttcagccggt tcgcagatgc gcgtgctgcc gaaaacaacc accgtgtggg
     1861 caatgccgcg ctccttgagc agcagttcgg ccttttcgta atcgagggcg agccgcgtgt
     1921 tccgtgtgtc gcggcgggca aggaaatcgg tgtcctggtc ggcttggcgg tagctcggac
     1981 tcgcgagaat ggccttgagg gctgcgggcg ccagcgggtc ttccgcggcc ggcttggggt
     2041 gctgcgaagg cagcggcgta cgccgctcga ccgggttcgg cggctgcgga atcttcgagc
     2101 gccgcggcgc cctgcgattt ttcttcacga tggcgagccg agcggtttac aggcttggtg
     2161 gcggtatctg gccttgcggg ggataggcgg gcagcggcgg cggcggcggg ggcagcccgc
     2221 cagggtgctt ctgcagcgcc cgcatcaggt attgcacgcc agcttccagc tgggcgtcgc
     2281 ggccggcgag cagctggccc ggatccgtat cgatgttcat gtccgggtcg acgccgtggt
     2341 tctcgataac ccactcggat cgcaatccgt acaggctgtc ttcgggcacc gtgatgtagc
     2401 cgccgtcaag cagggtccag tcaccacgaa tgccgcgaac acctccccag gtacgcgtgc
     2461 cgatgagcgg accaaggccg tatttccgga agtaataggg gaagatatcg ccgtcggacg
     2521 ccgaataatt gttgatcagg cacactttgg gtcccgaaat cagctgttgc ggaatggagg
     2581 tggatgctct ctcgcgattg gtatcgaggc ccaccaggat gcgtcgcagc cgttcgagaa
     2641 tcatctgatc gatgttgccg ccgccattcc agcggtcgtc gagaatcagc gcctgcctgt
     2701 cgatctgcgg atagaactgc cggatgaact gttccatgcc gagtgtttcc atgtccgaca
     2761 tgtagatgta ggcgatcttg ccgccggaca atttgtcgac caccgcccgg ttgtggtcga
     2821 tccagtcttt ttcgcgcagc gaaagttcgt tcttgatggg cttgaccgtt acatcgtggg
     2881 gttttccgcc agcccgctca accgtcaggg tcaccggctg atcgtcgact ccgaccagga
     2941 ggctgtaagg atctgccggc gctttcaggt cgcggccgtt gaccgcgagc agaacatcgc
     3001 cttcgcgcac gtccacgccc ggctccagca ggggactgcg atatttctcg cgcgtgttgt
     3061 cgcccggata gattttcgca aggctgtaat ggcctgatgg cgtcggcgcg aaatcgacgc
     3121 cgagcagagc tgtggacacg gtgtcggccg gattgtcctc gtcgccaccg cccacatagg
     3181 tgtgcgaatt gccgagttcg ccctgcatct cgccaatgag ataggtcaga tcctcgcgcg
     3241 acccgacgag cggaagaagc ttcgcataag catcatgcac cgcctgccat gccacgccgt
     3301 tcattttcgg attgacgaac aggtcgcgct cgagccgcca ggcgttgttg aacatctcgg
     3361 cccattcctg gaccggttcg atgcgtgtgc gcatatgcga gagatcgagc gatttggggt
     3421 ccggcttttt gtcgtcttcc ggcattttgg cgtcgatgat ggcgtagcct ttgccgtcgt
     3481 cgtcgctgtc gtcgccggcg ccagtctttt tgtagagtgc cttttctcca ttgtaggaca
     3541 ggctgtaact gtccaggtcc tcgaccagcg tggcgtcctt gcgcttttcg agattgtaga
     3601 cgtgcatggc ggacttcatg cgcggcaggg gcccttcgag catttgcggc tgcgcgatgc
     3661 ggtaatagac attgtcgtcg cgggcatcga tctgcgcgat gtcggccgcc ggaatcggca
     3721 gcggcacggc gcgcgccatc aggccgtcga aatcgattgc caccggcgcg gacgcgcctg
     3781 gtttccatgg cttgcttttc gagtctttct cggattcgaa agcgccctca tcggagcgcg
     3841 gcgcgaacgc cgaaggttcg ttcttgcgca acgtcgccac ataaatgccg tccatctcga
     3901 tcgatgcgac attgaactcg atttcgttgg ccgtcggatt ctcgtgacgc gtcgaaacga
     3961 aatacagata tttgccgtcc ggcgagaaca ccggatcggt gtcgttggca agcggctccg
     4021 agacgcgcac ggacctgccg ctgccgatat cgtccaccca gacgcccttt tgctgattgg
     4081 gcgcggtttt gctgtaggcg agccagcggc cgtccggtgc gaagctctgg tcgtggattt
     4141 cggaataggg atcctgcgcc acctgcgtcg cgttcccgct gctcgcatcg acgagccaga
     4201 gccggtgatt tccgtctgcg acggccagcc gtttgctgtc cggagaccac agggggccgt
     4261 agaggaagcc ggccgtgaaa tgggtgagga ttttctcggc gccgccggtg gcgggccgga
     4321 ctgcgatctg ctgctcgccg ctcacatcgg tggtgtaggc gatggtcttg ccgtcgggcg
     4381 accagcctgg atggtcctcg tcggcgttgc tggttcccgt gagattgcgt gtggcgccat
     4441 gctcggccgg cgcggtgaac agatcgccgc gcgccgagaa tatcgcgcgt ttgccgttcg
     4501 gcgacagcgc gtagtcggtg tcttgcgccg tgtcctggtc gcggatcgcc ttgctcgcgt
     4561 cggcatagcg gggtccggtg cgcgtgccgt catccggcac gctgacgttc agccggtgca
     4621 gcttctccga tggcagatcg atcacccaga gcgctccggc ctgcgcgaac acaatgccgt
     4681 cgccgccctt gccgcccagt ccgaggctgg ggaaatcgat gtcgtagttg gtgaagtgcg
     4741 tcacttcccg gaactgcttc gagtcgaggt cgtagaccca gatgttctcg cggcgcttcg
     4801 cgtcgtgatc cgacagatag tagattttcc gtccgaccca catcggcgcg gtgttggtgc
     4861 ccaaccacgt ggtgatgcgg gtcagcttct tcgtgtcgaa cgcgtaggtg tagacgtcct
     4921 ggccgaggcc gccattgtag cgtttccagg tgcggaaatt gcggaagatg cggttgtagg
     4981 cgatcgactt gccgtcgggg ctgtaagtca tgagcccgcc gcgatcgagc ggcaatggct
     5041 gcgcaagacc gccgcccgcc ggcacttcga acagccgccc gtaccagctg ttccacgcca
     5101 tgcgccggga aagaaagacg acattcttcg aatccggcgt ccaggtgacc accatgttgt
     5161 cggggcccca gcgggtaggc gcgctctcga ccacgtcgga atggaaggtg agccgcttcg
     5221 cagcgccacc gccggcgggc atcacataga cgtcctggtt gccctggtag aagccggtga
     5281 aggcaatcgc gcttccgtcg ggcgaatagc ggggcatcaa atcgtagccg ggatcggcgg
     5341 tcagccggct cgccgcgccg ccccagcgcc cgacctgcca cagattgccg tgcgcctcga
     5401 acacgatcga tccgccgtgc agcgccggaa accgcatgaa tgtccagtcg tgcgcgctgc
     5461 cgggttgggc ttgcgtgcac cccagcgcga tcaccgccgc gagcgccgcc cggcgccaga
     5521 cctggagctt catgcgtgtg tcctcgtgct gcagtgcctg ccgaatcgcg ggtcactcaa
     5581 gaaaccgcta acaaatcgcg ccgcgaggct ccttgcaagc ccggtcgccg ctcgtatcgg
     5641 aatgttgaac aaggccgcgc ggactgttac cgacgaaccg gcacctcgat gcagctgaca
     5701 ctgaaccatc tgatccgcaa gggtacgccc gcatcggcga tggcttcgtc gccgacatcc
     5761 atgccgcgaa atcgtgtcga cgcattcggc tagcgccaag ggccgttttc aggggtcatg
     5821 cagtccttcc aaacaatgcg cagtcggcag aagcgcgcca gttcttgccg ttcgcgtgca
     5881 caatgtcttc gatgcgagat aacctctagc gccgggcacc aacatcatca tcgacggcga
     5941 gccgcgaagc gttcgtgagt ccggtgatcg agccgcacct tgcactggcg gtgaatccgg
     6001 ggacgcctga tcgattacag cagcctcagc gcgtccgcgt ttgagtcctt gcggttgcgt
     6061 cctggcgccc gctttgcaat ggcccgtccg agcatgcgtt ccagtccggc aatgaattct
     6121 gcgttgccga gcggtctgcc cgttccttcg gaagcctgag gcggaaggaa gacttgctca
     6181 tcctttgcgt ccggcgcaat gagcctggca aagtcgggaa tgcgcgacag gaccggtgcg
     6241 acgttgaacc gggggaccag cgcgcattga tgaaggtggt gtactgccga tgcagccgcc
     6301 cagggcccgc gcgagacccg cttcgtcgct cggcgcgagg atgaggtgaa cttggttggg
     6361 catcaggcaa caggcccaga cggagacgga ggttttccgg cagtgcccgg cgagcaggcc
     6421 gcgatagatt tcctggtcgc cgggttcgaa ggagatgggt tcgcggctat cgcggcgctg
     6481 cgtgacgtga tgcgggcacc cgggggacga cgatgcgggt gagacgggcc atcgcaggga
     6541 tataccgccc ccggcgcgtc tggcgtagct cccgtcaacg ccattccgcg cgtctttgtt
     6601 tgtcgccaag gcgagctacg gcaaagttcg gaaaacggat cagacgtccc cgtattccaa
     6661 aaaagacgac cgcgatcccg ttgccgcgaa agtcaaaggc ttagaggccg ttgccttggt
     6721 gctacggccg tgctacacgg cgtcccatga aacaggtgac gctccgcgaa gccaatcagc
     6781 agttctcccg gcttgtccgc gaagtcgaag aaacgggcga acgcgtgctg gtgctccgcg
     6841 acggcaaacc cgccgtcgag atcgctccaa cggccgccag acgggccatg cgcaagctga
     6901 cgcgcgaaca ggagaccgca ttcggcgcgt ttgtggattc cgcggtgaaa cgtcccggca
     6961 gttccaaagg cgcgcgacgc tggtcgcggg acgaactgca tgaacgctga gcggttcacg
     7021 ctcgacacca acatcctcgt ctacgccgtg gacgcgcgcg acaggcgcaa gcatgcgctc
     7081 gccgtggaca ttctgaaagc cgccgtccgg ctcgattgtc ccctcgcgtt gcaggcgatc
     7141 ggcgaattct atgttgcggc cgtcggcaag gtgaaactcg cgccagatga tgcgcgcgag
     7201 cgggccttgc agctgatgac gggattcgaa gccttcgcgc attcggcgag cgccgtccgc
     7261 gccggactgg aagaatctgc gcgtggccgg ttctcgtttt gggatgcggt actgctcgct
     7321 tcggccgccg aagccggatg ttcggtcatt ctttccgaag acatgcatga cggcgcgcgg
     7381 ctgggctcga tcgccatttg caaccccttc ggagcaaagg ggctgtctcc cgcggcacgc
     7441 gaactctttg gataggcgga aacgcgagaa atggcttgcc gctgccgcct ggtgcacgct
     7501 tgcgcttgcg gccctgcccc cattgtccca gccgtttccg tcggatgcgg aaattttgca
     7561 gggttcacac cctgcggacg aagccgaagc atccttgagg agatggtgtg atcattcgat
     7621 caaagacctg ggacattccg cctttcgtca cggcattcga aaccgcggcc atgtttggaa
     7681 cccaggttgc gcttaccatc tgggctgtgg gcaatgcccg cagataccgc acatatgagc
     7741 gcgttcgagg cgatacacgc ttcacttgcg aggctgggcc gtccacatat gacgaggatt
     7801 ttggaagttg cagattcgcg ctattccgtg ccctcagttt ttttccttca gcctggccgc
     7861 cagttcgacg ctccggggtg cggccatgcc gccgaaacgt gcgtcgattt gctcaagcag
     7921 gccgcgggcg gagtcgcgct cgcccgcctg gatgaacgac tcgacctgcc gcagtttcgt
     7981 ttccagctct gcctggatcg ccgaccggca gccggcgagc ctggcaggat cggacggcgc
     8041 cggattcaat agcgtgtcga gagccctgga gagcgccacc gagtcgacga cgtcatggtc
     8101 ggcgaaggcc gagatgtggt cttcgacgtt cgacacgcac cagtcccgca tcgaatgcat
     8161 gctggccata tccatggcga ggtgaaaact gtcgcgcgcg cccgtgacg
//
LOCUS       DASZMC010000531         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_788014,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000531 DASZMC010000000
VERSION     DASZMC010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788014"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="VG274_10845"
     CDS             <1..1369
                     /locus_tag="VG274_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07197.1"
                     /translation="FALGPNINLPMQEDAAAPAWAALGKASALESHASPQEREWIDAL
                     AQRYSADPEADRKPLDEAFAQAMGKLWRAHGDNLDAGVFYAEAMMDTQPWDYWEHDGE
                     TPKGHGQEIVSTLENLIKQEPSHPGALHLYIHAVEATTHPERAEAVADRLEPLMPGAG
                     HIVHMPSHIYYRVGRYADAVHVNELAAKADEDYIAACKAQGFYPLAYYSHNIHFLWTS
                     SEMMGRYQAAIDAARRVVKASDDGPAEQVPPLQLYLFTPIVTDIRFGKWDAALAESRP
                     APNLRLASAMWLYASGFAHANKHNFAAARKDRAELEAMIDRKDFTAIDAFGVPGTQMA
                     KLGEALLDGEILRASGHLARAIAQYGKAREIERALPYTEPPYWHQPVSHILGAALLAA
                     HRPAGAEAVYRDSLKSYRIDGWALYGLAQALEAQHKTKEAAGIRADFDRAWAMADVKL
                     ASSRF"
     gene            1427..1639
                     /locus_tag="VG274_10850"
     CDS             1427..1639
                     /locus_tag="VG274_10850"
                     /inference="COORDINATES: protein motif:HMM:NF022456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HWE07198.1"
                     /translation="MRKLVGTLGMIVWLAVYAIVAAGIGVRVLPRAAWPIILVYYALA
                     GTLWIVPVGLMLPWMHRTSRRGPEAS"
     gene            1639..2250
                     /locus_tag="VG274_10855"
     CDS             1639..2250
                     /locus_tag="VG274_10855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWE07199.1"
                     /translation="MRIVMTRHGHVEGIMPERFRGRADVPLTERGMGEARMVAGRIAR
                     DWMPAAIYSSPLKRCMSTAEQIAQACRATIVPLEDLNDLDYGDLQWKPFEEAKREYAE
                     LFQTWFAAPQQVRFPNGESLQDLGARTSNVLRLLRERHGDDVVVLVGHDSVNRAILLQ
                     LLDQPLSAYWRLAQSPCSINEIVMSGRLTRVESVNDTSHLRGA"
BASE COUNT          477 a          772 c          748 g          434 t
ORIGIN      
        1 ctttgcgctc ggccccaaca tcaacctgcc gatgcaggag gatgcggctg cccccgcctg
       61 ggccgcgctc ggcaaagcgt ccgcgctgga gagtcatgct tcgccccagg agcgcgaatg
      121 gatcgatgcc ctcgcccagc gctactccgc cgatcccgaa gcggaccgca agccgctcga
      181 cgaagcgttc gcgcaagcca tgggaaaatt gtggcgggcg cacggggaca atctggacgc
      241 cggcgtgttc tacgcggagg cgatgatgga tacgcagccc tgggattact gggaacacga
      301 tggcgagacg ccgaaaggcc acggtcagga aatcgtatcc acactcgaaa acctcatcaa
      361 gcaggagccc agccatccgg gtgcgctgca tctgtatatc cacgcggtgg aggccacgac
      421 gcatccggag cgcgccgaag ccgtcgccga ccggctcgag ccgctcatgc cgggagccgg
      481 tcacatcgtg cacatgccgt cgcacattta ttaccgtgtg gggcgctacg cagacgccgt
      541 gcacgtcaac gaactcgctg ccaaggccga cgaggattac atcgcagcct gcaaggcgca
      601 gggtttctat cccctcgcct actactccca caacatccat ttcctgtgga catcgtcgga
      661 aatgatgggc cgctaccagg cagcgatcga cgcggcgcgc cgcgtggtga aagcttccga
      721 cgacggtccc gccgagcagg tgccacccct tcaactttat ctcttcactc cgatcgtgac
      781 cgatatccgg tttggaaaat gggatgcggc gctcgccgaa agccggcccg cacccaatct
      841 tcggctggct tcggccatgt ggctctacgc aagcggcttc gcgcacgcca acaaacacaa
      901 ctttgccgct gcacgaaaag atcgcgccga actcgaggcg atgatcgacc gcaaggactt
      961 caccgcgatc gacgcgttcg gcgtgcccgg cacgcagatg gcaaagcttg gcgaggcact
     1021 tctcgacggc gaaatcctgc gggcaagcgg ccatctggcc cgcgcgatcg cgcagtatgg
     1081 aaaggcgcgc gaaatcgagc gcgcgctccc ctataccgaa ccgccttact ggcaccaacc
     1141 tgtgagccac attcttggtg cggcgctgct tgccgcgcac aggcccgccg gggcggaagc
     1201 cgtgtaccgc gacagcctga aatcgtatcg catcgacggc tgggcgcttt acggtcttgc
     1261 ccaggcgctt gaggcgcagc acaagacgaa ggaagccgcc gggatccgcg ccgatttcga
     1321 cagggcgtgg gcgatggcgg atgtgaagct cgcgtcgtcc agattctgaa ccgacgccgc
     1381 agggaatgcg catgatccga cagtatttgc gcccatgacg gcgcacatga ggaaacttgt
     1441 cgggactctc ggcatgatcg tctggctggc ggtctacgcg atcgttgcag ccggaattgg
     1501 cgtgcgggtc ctgccccgag ccgcatggcc catcatcctg gtctattacg ctctcgcggg
     1561 tacgctgtgg atcgtgcccg tcggcctcat gctgccatgg atgcatcgca cttcccgcag
     1621 agggccggaa gcaagttgat gcggattgtc atgacgcggc acggtcatgt cgaaggcatt
     1681 atgcccgagc ggtttcgcgg gcgcgccgat gttccattga ccgaacgcgg aatgggtgaa
     1741 gcccgcatgg ttgccggacg catcgcacgc gactggatgc cggcggcaat ctactccagc
     1801 ccgctgaaga gatgcatgag cactgccgaa cagattgcgc aggcatgccg ggccacaatc
     1861 gtcccgctgg aggatctcaa cgatctcgac tatggcgatc tgcaatggaa gccgttcgaa
     1921 gaggcaaaac gcgaatatgc ggaactgttt caaacatggt tcgccgcgcc gcaacaggtg
     1981 cgctttccga atggcgaatc ccttcaggat cttggcgcgc gcacgtcaaa cgtcctccgg
     2041 ttgctgcgcg agcggcacgg cgatgacgta gtggtgctcg tcggacacga cagcgtcaat
     2101 cgcgccatat tgcttcaact gctggatcag ccgctttcgg cctattggcg gctcgcccag
     2161 tcgccgtgtt cgatcaacga gatcgtgatg tcgggacggc taacccgtgt cgagagcgtg
     2221 aacgacacga gccatttgcg aggggcgtga actccggtca gccgtcgccg gactcgttcg
     2281 gcttcgtctg cagctggacg aaattctgca atcccatctg ttcgatgagc cggaactggg
     2341 tctgcagcca gtcgatatgc tcttcctcgc tctcgagtat ctggacgaac aattcgcgcg
     2401 agacatagtc gcccacccgt tcacaaagtc c
//
LOCUS       DASZMC010000532         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4621416,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000532 DASZMC010000000
VERSION     DASZMC010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4621416"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /gene="pbpC"
                     /locus_tag="VG274_10860"
     CDS             complement(<1..1890)
                     /gene="pbpC"
                     /locus_tag="VG274_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HWE07200.1"
                     /translation="MSASLLLGLAVTLAAVDWVCPPDLSRYRRLSPEVVDRRGALLRP
                     FLSKDGYWRLATHVADVSPRYLALLKAYEDKRFDSHPGIDMLALARAAFQYARAGHVV
                     SGASTLTMQVARLLEPRPRGVASKLIQMARALQLEERYTKDEILSIYLTLAPMGGNLE
                     GVRAASLAWFGKQPSELDLGEAALLVALPQSPARLRPDRHMLAARSGRDKVLSRMVQD
                     GVLASADAALAERESVSSLRLAMPLAAPHLAQELVRQVRAPARIASTLDASLQGAVER
                     LATREKAYLADRSSLAVVVVENRTRDVLAYLGGTNYWGASGQVDLAARPRSPGSALKP
                     FIYGLAFDDLILHPSTMMEDAPMNFGDYAPRDFEGAFQGAVTARDALRMSLNVPAVMV
                     LDRIGPLRFSLALENAGAHLAYPTHEAAPSLPIALGGLGISLRDITMLYAGIADGGLV
                     QALRTRTDAPPDTGHRLFGPAAAWYLRDILLGASLPEGWAMGQGLARAHAVAFKTGTS
                     YGFRDAWSIGFSNDYTVGVWVGRADGAPSADSIGREAAAPILLKVFELLPPDRRPPAS
                     RPASAILARSTGDLPPGLRVFTRDSESAAVRQTNIVPPSIAFPPNGATVPLPTPQAKD
                     PTILFR"
BASE COUNT          359 a          641 c          699 g          309 t
ORIGIN      
        1 cctgaagaga atggtcgggt ccttcgcttg cggcgtggga agtggaacgg ttgcgccatt
       61 cggcggaaac gcgatcgatg gtggcacgat attggtctgc cgtacggcag cggattcgct
      121 gtcgcgggta aagacgcgca ggcccggcgg cagatccccg gtcgagcgcg cgaggatcgc
      181 gctggccggc cgcgacgccg gcggtcggcg gtcgggcggc aggagctcga acaccttgag
      241 caaaatgggc gcggccgcct ctcttccaat gctgtccgcg ctcggcgcgc catctgcgcg
      301 accgacccag acgccgacag tgtaatcgtt cgagaagccg atcgaccacg catcgcggaa
      361 gccataggag gtgcccgtct tgaacgcgac cgcgtgagcg cgggcgagcc cctgccccat
      421 cgcccagcct tcgggcagcg acgcgccgag aagaatgtcg cgcaggtacc atgcggcggc
      481 cggcccgaag aggcgatgcc ctgtatcggg cggcgcgtct gtgcgggtgc gcagtgcttg
      541 cacgaggccg ccatcggcga tgcccgcata gagcatcgtg atgtcgcgaa ggctgatgcc
      601 gagcccgcca agcgcgatcg gcagcgacgg cgctgcctca tgggtgggat aggcgagatg
      661 cgccccggca ttctcgagtg cgagcgagaa acgcagggga ccgatgcggt cgagcaccat
      721 gaccgccggc acgttgagcg acatgcgcag cgcgtcgcgc gcggtcaccg cgccctggaa
      781 cgcaccttcg aaatcccgcg gtgcgtagtc gccgaaattc atcggcgcat cttccatcat
      841 ggtcgaggga tggaggatga gatcgtcgaa ggcgaggccg tagatgaaag gcttgagcgc
      901 cgagcccggc gagcgcggac gcgcggcaag gtcgacctgt ccggacgcac cccaataatt
      961 cgtgccgccg agataagcga gaacgtcgcg cgtgcgattt tccaccacga cgacggccag
     1021 actcgagcgg tctgcgagat acgccttctc gcgcgtcgcc agtctttcga cggcgccttg
     1081 cagcgatgca tcgagcgtgc tcgcgatccg cgccggcgcg cgaacttggc gcacgagttc
     1141 ctgcgccaga tgcggcgcgg caagaggcat ggcgagcctc agcgacgaca cgctttcccg
     1201 ctcggccagc gctgcgtccg cggacgcgag cacgccgtcc tggaccatcc gcgatagcac
     1261 cttgtcgcgt ccggatctgg ccgccagcat gtgccggtcc gggcgcagcc gcgcgggcga
     1321 ttgcggcagc gcgacaagca gcgcggcttc tcccagatcg agttcggaag gctgtttgcc
     1381 gaaccaggcg agcgaggcgg cgcgcacgcc ttcgagattg ccgcccatcg gcgccagcgt
     1441 cagatagatg gaaaggattt cgtccttcgt atagcgctct tcgagctgca gcgcgcgtgc
     1501 catctggatg agcttgctgg ccacgccgcg tggcctcggt tcgagcaggc gcgccacctg
     1561 catggtgagc gtcgacgcgc cggagacgac atggccggcg cgcgcgtatt gaaaggcggc
     1621 gcgcgccagc gccagcatgt cgattccggg atgcgaatcg aagcgcttgt cctcataggc
     1681 cttgagcagc gcgaggtagc gcgggctgac atcggcgaca tgggttgcga gtcgccaata
     1741 gccgtccttc gacaggaacg gcctcagcag cgcgccccgg cgatcgacca cttcgggcga
     1801 gagcctgcga tagcgcgaca gatccggcgg gcagacccaa tcaaccgcgg caagcgtgac
     1861 ggcgagccct aggagcagag acgcgctcat ccgccttgtg aaaatcatgt gcatcaatct
     1921 gcgcatgcgg cgagagagac gattcggcga gttttcctcg gcagtatcgc acaacggaaa
     1981 caatttggcg cgtttctttt gacaatcg
//
LOCUS       DASZMC010000533         5582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_788340,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000533 DASZMC010000000
VERSION     DASZMC010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788340"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /gene="zapE"
                     /locus_tag="VG274_10865"
     CDS             complement(<1..1107)
                     /gene="zapE"
                     /locus_tag="VG274_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HWE07201.1"
                     /translation="MSEDLEFDAAQLAVARGLNALSTALKSWRSGRPFLFGRRRAPPR
                     GIYLWGAVGRGKTMLMDAFFARAPLARKRRVHFNAFMADTHARIHEWRKLDPEARAQR
                     AEFIREAGDDPIPPIARAIAAEAPLLCFDEFQVRDVADAMILGRLFEQLFARDVVIVA
                     TSNTRPDRLYQGGLNRQLFLPFIALIQQMLDVVELGGARDWRLGRMAGVGLYLTPLGP
                     EADAALDMAWLQLTDGTCGKAESITLLGRRIAIPRAAHGAARFSFDELCARPLAAADY
                     LAIARRYHVLMIKRIPLMRAEMRDEARRFIILIDTLYDQNVKLVCSAVARPDALCVEG
                     DSAEAFRRTASRLTEMQSLDYLERTHGLRAARPAR"
     gene            complement(1104..1976)
                     /locus_tag="VG274_10870"
     CDS             complement(1104..1976)
                     /locus_tag="VG274_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWE07202.1"
                     /translation="MHAAIRPRRSILYMPGSNARALQKARTLPADVLILDLEDAVAPD
                     MKEQARVQVGEAVRARAFGSREVVIRINALSTEWGNADLNVATEARPDAILLPKVSRA
                     DDLRQADARISGRGIALWAMIETPLAILNIASIADAGGVLACFVMGTNDLVKDMRGQH
                     MPRRENLAPALALAVTAARASGLAIIDGVHNDIADLDSFRDACIQARAFGFDGKTLIH
                     PSQLAICNDVFAPARDEVEAARKIIAAFERPENSGKGAIALDGHMVERLHAEAARGAV
                     ALAEAIAARENTGS"
     gene            complement(2524..3549)
                     /locus_tag="VG274_10875"
     CDS             complement(2524..3549)
                     /locus_tag="VG274_10875"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWE07203.1"
                     /translation="MSYNVAVVGVTGIVGREMLGVLAERKFPVADVAALASTHSIGTE
                     VSFGERTLKVKALDYHDFAGTDIVLMSAGSAVAKQWAPKIAATGALVIDNSSCWRMDR
                     EVPLIVPEVNAKVLEDGIPKGIIANPNCSTAGVVVALKPLHDEAAIKRVVVATYQSVS
                     GAGKAAMDELFRQTRAVFVADPIEGGKFTKQIAFNVIPHIDDFLDSGYAKEEWKMMVE
                     TQKILDPDIQLTATCVRVPVFIGHSAAVNIEFERPITAQRARAILREAPGILVVDKRE
                     DGGYVTPVEAAGEDATYVSRIRKDPTVEHGLSLWVVTDNLRKGAALNAVQIAECLINR
                     RLLKKAA"
     gene            complement(3635..4018)
                     /locus_tag="VG274_10880"
     CDS             complement(3635..4018)
                     /locus_tag="VG274_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07204.1"
                     /translation="MRLDVLTCAFLLAAIPATARPLATPKPLPPAPRPLLDLSGLKFF
                     HEQQPSDSGTTGSPAGTGAQWSGSRDVTGLSLGPLRAQVGADDKPRDGLQSYELQGMD
                     LLGNAMSKNNTGQTAKLLFVWPTDQ"
     gene            complement(4115..4375)
                     /locus_tag="VG274_10885"
     CDS             complement(4115..4375)
                     /locus_tag="VG274_10885"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE07205.1"
                     /translation="MLRENGSKFLGGRGPTYPDLSRFQTVGGLGYAFPKAMARLERRH
                     RGLTVDHGRIAARPRIRAYLNSPRRLALSKNGIFRHYPELDG"
     gene            complement(4407..>5582)
                     /locus_tag="VG274_10890"
     CDS             complement(4407..>5582)
                     /locus_tag="VG274_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07206.1"
                     /translation="IAVLLDLILMAVSGMHSLRTGKIPMDEGQTSWRAARLLWRGENP
                     YGVGALTDLGAWQARAQERKATGVATGLAGAKVGATLAKYDATLDEGLRRELLPVPAK
                     PAGAAMRELLVCGYKYGPLILLVTALAAPFGIPALVLLLNGLACFALYAVHWRILSRI
                     AAPALSLAAAAMLALLLDRHITRNYIDRSATDVWALLFGSLAVLACISRRPLAMAAAI
                     ALAVASKSLPGLLFAPLLLKFRSPIPALLFVGLTGAIYLPWLLWDPQGILYNVFLWPF
                     LMARDYTSWEFFAPPAAALAVQGVGACALAVLWLRYLLGRETRLFWTLAVSNLVVLLA
                     AGFLRNGYVPWVSLWVVAAIAEAFRERSLVSSRRAEAPIVVAEREMQPISVAAARNS"
BASE COUNT         1011 a         1818 c         1799 g          954 t
ORIGIN      
        1 ccttgccggc cgcgccgcgc gcagcccatg cgtgcgctcc agatagtcga ggctctgcat
       61 ttccgtaagc cgcgacgcgg tgcggcggaa cgcctccgca ctatctccct ccacgcacag
      121 cgcgtccggc cttgccacgg cggagcatac gagtttcacg ttctggtcgt agagcgtgtc
      181 gatgagaatg atgaatcgcc gcgcctcatc gcgcatctcc gcacgcatca gcggaatgcg
      241 cttaatcatg agcacatgat aacgtcgcgc aatcgccagg taatccgccg ccgccagagg
      301 ccgcgcacaa agctcgtcga aagaaaaccg cgccgcgccg tgcgcagcgc ggggaatggc
      361 aattctacgg ccaagcagcg tgatcgattc cgcttttccg cacgttccat ccgtcagctg
      421 caaccacgcc atgtcgagcg ctgcgtccgc ttccggtccc agcggcgtca gatagaggcc
      481 gacgccggcc atgcggccaa gccgccagtc gcgcgcgcca ccgagctcca cgacatcgag
      541 catctgctga atgagtgcga tgaaaggcag aaagagctgg cggttcagcc cgccctgata
      601 gagccggtcc ggccgcgtgt tcgaggtggc gacgatcacg acatcgcgcg cgaaaagctg
      661 ctcgaacagg cggccgagaa tcatcgcgtc ggccacgtcg cgcacctgaa actcgtcgaa
      721 gcagagaagt ggtgcctccg cggcgatcgc gcgcgcgatg ggagggatcg gatcgtcgcc
      781 cgcctcgcgg atgaattcgg cgcgttgcgc gcgcgcctcc ggatcgagct ttcgccattc
      841 atggatgcgc gcatgcgtgt cggccatgaa ggcattgaag tgaacgcggc gtttgcgcgc
      901 gagcggcgcg cgcgcgaaga acgcgtccat cagcatggtc ttgccgcggc ccaccgcgcc
      961 ccacagatag atcccgcgtg gtggcgcacg ccggcgcccg aagaggaaag gccggccgga
     1021 gcgccagctt ttcagcgcag tcgaaagcgc gttcagccct cgcgcgacgg caagctgcgc
     1081 tgcgtcgaat tcgaggtcct cgctcaagag ccggtgtttt cccgtgccgc gatggcctcc
     1141 gcgagcgcga ccgcgccgcg cgcggcctcc gcatgaagcc gctcgaccat gtgcccgtcg
     1201 agggcgatcg cgcccttgcc ggagttctcc ggccgctcga acgccgcgat gatcttccgc
     1261 gccgcctcga cctcgtcgcg agccggcgcg aagacgtcgt tgcagatggc gagttgggag
     1321 ggatgaatca acgtcttgcc gtcgaagccg aaagcccgcg cctgaatgca agcatcgcgg
     1381 aagctgtcga ggtcggcgat gtcgttgtgg acgccgtcga tgatggcgag accgctggca
     1441 cgcgccgcgg ttaccgcaag cgccagcgcg ggtgcgagat tctcgcggcg aggcatgtgc
     1501 tgcccgcgca tgtccttgac gagatcgttg gtgcccatga cgaagcaggc gagcacgccg
     1561 cccgcatccg cgatcgaggc aatattgaga atggcgagcg gcgtctcgat catcgcccag
     1621 agcgcgatcc ctcgtccgga tatccgtgca tcggcctggc gcagatcgtc tgcgcgcgag
     1681 actttgggaa ggagaatcgc atcgggccgc gcctcggttg ccacgttgag atccgcgttt
     1741 ccccattcgg tcgagagcgc attgatgcgg atgacgacct cgcggctccc gaacgcgcgg
     1801 gccctgaccg cctcgccgac ctggacgcgt gcctgttcct tcatgtcggg cgcgacggcg
     1861 tcttcaagat cgaggatcag cacatccgcg ggcagcgtgc gcgccttctg gagcgcgcgg
     1921 gcattcgagc ccggcatgta gaggatggag cggcgcggcc ggatcgcggc atgcatcggg
     1981 atttccccgc aagttttcga ggcgacacct agcccagcca atgccagccg ccaatacctg
     2041 ctcacctttc gacaagctcg gggtgaggat ttattgcctc gtcctgagcc tgccgaagga
     2101 caagagaatc gtgaacatcc tttctgtgca gtccgaagtc gtcgccaagc ccggcatcgg
     2161 cgaggcaatc gcacgcatgc ttttgccgct cgccgatatc gttacgccca atgcgttcga
     2221 gctgacgcag ctcgcggctt tgccggtgcg caatcccgac gaagccggaa cagccgccgc
     2281 acagctcggc cgtccgcttg tcgttgcgac atcgattccg gatggagttg gccgcatcgg
     2341 cgctcttgca tcgacgcgca acgaatgctg gttcgcgtcg acgccgcgat gttccgcacg
     2401 gcgcgggcga ccttttcgcg gcgctctttc tgacggcaag gctttccggc ctgcccgtgg
     2461 cccagtcgct cacagcgact ttggcatctg tctttcacat cctctggggc agccgcgtac
     2521 ccgtcacgcc gccttcttga gcagcctccg gttgatcagg cattcggcga tctgcaccgc
     2581 gttgagtgcg gcgcctttgc gcagattgtc cgtcaccacc cagagcgaga gcccgtgctc
     2641 gaccgtgggg tccttgcgga tgcgcgacac gtaggtggcg tcttcgccgg ccgcttcgac
     2701 gggcgtcacg tagccgccgt cttcgcgctt gtcgacgacg agtatgcccg gcgcctcgcg
     2761 tagaatcgcc cgcgcccgct gcgccgtaat cggccgctcg aattcgatgt tcacggctgc
     2821 agagtgcccg atgaacacgg gcacgcgcac gcaggtcgcg gtgagctgga tatcgggatc
     2881 gagaatcttc tgcgtttcga ccatcatttt ccattcctct ttcgcgtaac cggaatcgag
     2941 gaagtcgtcg atgtgcggga tgacattgaa ggcgatctgc ttggtgaact tcccgccctc
     3001 gatcgggtcg gcgacgaaga cggcgcgggt ctgccggaac aattcgtcca tcgctgcctt
     3061 gcccgcaccc gaaaccgact gatacgtcgc aacgaccacc cgcttgatgg cggcttcgtc
     3121 gtggagcggc ttcagcgcaa caacaacccc ggcggtcgag cagttgggat tggcgatgat
     3181 gcccttcggt atgccgtcct cgagaacctt cgcgttgacc tcgggcacga tcagcggcac
     3241 ttcgcgatcc atgcgccagc acgaggaatt gtcgatgacg agcgcgccgg tggcggcaat
     3301 ctttggcgcc cattgcttcg cgacggccga acctgcggac atcaatacga tgtcggttcc
     3361 ggcgaaatcg tgatagtcga gcgccttcac cttcagcgtg cgctcgccga aggaaacttc
     3421 ggtaccaatc gagtgtgtag aagcaagcgc cgcaacatcg gccacgggga atttccgctc
     3481 cgcgagcacg cccagcatct cgcggccgac gatgccggtt acgccgacga cggcgacgtt
     3541 gtaggacatg agatcgttcc ctgtttttct gcagcgcagt gcggcgcgcg agagcgttta
     3601 ctccaactct cggccgcaga gaagcggcga cgggctattg gtccgtcggc cagacgaaaa
     3661 gaagtttggc cgtttggccc gtattgtttt tcgacatggc gttgcccaaa agatccatgc
     3721 cctgcaattc atagctctgg aggccgtcgc gcggcttgtc gtcggcgccg acctgcgcgc
     3781 gcaacggacc gagcgaaagc ccggtgacgt cgcggcttcc cgaccattgc gcgccggttc
     3841 cggccggtga accggttgtg ccggagtcgg aaggctgctg ctcgtgaaag aatttgagac
     3901 cgctcagatc gagaagcggc ctcggggctg gcggcagggg tttgggggtc gcaagcggac
     3961 gtgccgttgc cggaattgca gcgagcaaaa aggcgcaggt gagtacatcc agacgcatga
     4021 cgcgtggctc ccgctctgcc gaattctacc tctttttttg ccggagtctc acaccggccc
     4081 tgcctgatca ttaaaaattt atacctgttc cgggtcagcc gtccagctcc ggatagtgcc
     4141 ggaatatgcc atttttgctg agggcgagac gccgcggcga attcagatag gcccggatgc
     4201 gcggccgggc ggcgatccgg ccgtggtcga ccgtcaggcc ccgatgccgg cgttccagcc
     4261 gcgccatggc cttcgggaac gcgtaaccca agcctccgac ggtctgaaaa cgggacagat
     4321 cgggataggt gggcccgcgt ccgccgagaa atttcgagcc gttttccctc agcacgccgc
     4381 caaaacaggg tcaagacatc cgcgcgtcac gaattgcggg ccgcagccac gctgatgggc
     4441 tgcatctccc gttccgctac gacaatgggc gcttcggcgc ggcgggagga aaccaggctg
     4501 cgctccctga acgcctcggc gattgccgcg acgacccaca gcgacaccca tggaacatag
     4561 ccgttgcgca gaaatccggc tgccagcagt acgacgagat tggagactgc gagagtccag
     4621 aacagccgcg tttccctgcc gagcaggtag cgcagccaca gcacggccaa cgcgcacgcg
     4681 ccaacgccct gcaccgcgag ggcggcggcc ggcggcgcga agaactccca cgatgtgtag
     4741 tcccgggcca tcaggaacgg ccaaagaaag acattgtaca aaatgccctg cgggtcccag
     4801 agcagccacg gcaaatagat ggctccggtc aggcccacga acagcagcgc cggaatgggc
     4861 gagcgaaact tgagcaggag cggcgcgaac agaaggccgg gcaggctctt gctggccacc
     4921 gccagcgcga tcgcagccgc catcgcgagc ggccttcgcg aaatgcaggc gagcaccgcc
     4981 agcgatccaa acagaagcgc ccagacatcg gtggccgagc ggtcgatata gttgcgggtg
     5041 atatgacggt cgagcagcag cgccagcatg gcggcggcag cgagcgacag ggcgggcgcc
     5101 gcgattcgcg acaggatgcg ccagtggacg gcgtagagcg cgaagcaggc gagcccgttc
     5161 agaagcaaaa ccagcgccgg aattccaaag ggtgcggcaa gcgccgtgac cagaaggatg
     5221 agcggcccat atttgtatcc gcacaccagc agctcgcgca tcgccgctcc ggctggtttg
     5281 gcgggaacag gaagcaattc gcgacgcagc ccctcatcca gcgtcgcgtc atatttcgcg
     5341 agagtggctc cgactttcgc gccggccagt ccggtcgcta cccccgttgc tttgcgttct
     5401 tgcgcgcggg cctgccaggc gccgagatcg gtcagggctc caacgccgta ggggttctcg
     5461 ccgcgccaca ggagccgcgc ggcgcgccag ctggtctgtc cctcgtccat gggaattttg
     5521 cccgtccgca gactgtgcat gccgctgacg gccatcaaga tcagatcaag cagcacagcg
     5581 at
//
LOCUS       DASZMC010000534         4812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2136821,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000534 DASZMC010000000
VERSION     DASZMC010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2136821"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VG274_10895"
     CDS             complement(<1..221)
                     /locus_tag="VG274_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07207.1"
                     /translation="MLTRKQHELLMFIHERVRETGVPPSFDEMKDALDLKSKSGIHRL
                     VTALEERGFLKRLEKRARALEIIRLPENVA"
     gene            complement(353..1540)
                     /gene="glp"
                     /locus_tag="VG274_10900"
     CDS             complement(353..1540)
                     /gene="glp"
                     /locus_tag="VG274_10900"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWE07208.1"
                     /translation="MISVEDAVRRIGAAVAPLGSERVPLAIAHRRVLSADAIAALDQP
                     PHPISAMDGYAVRLADTHTVPVTLRVVGAAPAGHPFAGSVRAGEAVRIFTGGVVPEGA
                     DAIVIQENTEAKDDRATLRAPAAARHIRAARFDFQKRDVLCAAGRKLTARDLALLAAG
                     GISIVDVQRRPVIAFAATGDELAPPDAPQRPGGIVASSGYALAALIEIWGGQPLDLGI
                     FPDTIEALQRIPELASPADVVVTMGGASVGDHDLVQRALEPKGFTLDFWRIAMRPGKP
                     LIFGKLGEKPLLGLPGNPVSSYVCAVLFLQPLIAGLLGVSFSRPLQQARLAGALPAND
                     ARQDYLRGRLIRRDGELWVEPFTIQDSSMQSALAAADALIVRRPHAAAAQAGEPVEVL
                     LLD"
     gene            complement(1537..2025)
                     /gene="moaC"
                     /locus_tag="VG274_10905"
     CDS             complement(1537..2025)
                     /gene="moaC"
                     /locus_tag="VG274_10905"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWE07209.1"
                     /translation="MSKLTHLDEHGAARMVDVSAKEASVREAQAEAIVVLSADAFAAV
                     CGGAAPKGDVIAAARLAGIMAAKKTSELIPLCHPIALTQAAVEIEHLPERHALRIVTS
                     TKTKAETGVEMEALTAAAIAALTIYDMTKAIDRGAVIESVRLLSKSGGKSGSFAAPRR
                     DR"
     gene            complement(2274..3149)
                     /gene="trpC"
                     /locus_tag="VG274_10910"
     CDS             complement(2274..3149)
                     /gene="trpC"
                     /locus_tag="VG274_10910"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001370.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWE07210.1"
                     /translation="MTILDAIAVYKREEIARAKHEMPESMLDEQARTAGPPRGFRAAL
                     AACRDAGYYGLIAEIKRASPSKGLIRADFDPGTIARAYRDGGAACLSVLTDGPSFQGA
                     PAHLETAHAAVTLPVLRKDFLFDPYQIVQSRALGADCILIILAAVDDDDARALLAAAR
                     HWGMDALVEIHDEIERDRALALGAEMIGINNRDLKTFVTDPAVTLRLAPAIPRHVLVI
                     GESGLSSPSDLRRLADAGVTTFLIGESLMREKNIAAATRALLRDSEYSALRKKGSRGD
                     GEVLNRQGAKTPRRT"
     gene            complement(3146..4180)
                     /gene="trpD"
                     /locus_tag="VG274_10915"
     CDS             complement(3146..4180)
                     /gene="trpD"
                     /locus_tag="VG274_10915"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWE07211.1"
                     /translation="MSADEDFPSLLKRLAEGRSLSAESSLRAFGAIMAGEVSEARMAA
                     FLTALTLREPTVAEIVGAARAMRAAMRTIEAPAGAIDLCGTGGDGRGTLNISTAASFV
                     VAACGVPVAKHGNRNMSSRAGAADVLEALHVRIDLTPQAAQTCLDRLGICFLFAPAYH
                     PAMKHVAPVRKELGFRTVFNLLGPISNPAQVHRQVLGVFAERWVEPLANVLVQLGTEH
                     AWIVHGRDGMDELTTTDASLVVEIAQGKIRRFQLTPEDVGLGRVSPEDVKGGDAAFNA
                     NELEAMLQGDGNCAYRDIVVLNAAAALVVGGKAADISEGACQADAAIRDGGAFDMLAR
                     LRTTTQALAS"
     gene            complement(4190..4774)
                     /locus_tag="VG274_10920"
     CDS             complement(4190..4774)
                     /locus_tag="VG274_10920"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWE07212.1"
                     /translation="MLLLIDNYDSFTYNLVHYLSELGAEVDVRRNDEIAVRDALELRP
                     EAIVLSPGPCDPDRAGICLGLIRAADGRVPILGVCLGHQAIAQAYGGSIVRAPVPMHG
                     KVSRIHHDGSGVFRGLGNDFEATRYHSLTVDPESVPTCLAVNAGTSDGVIMGVMHKEH
                     PVHGVQFHPESIASENGHALLRNFLALARERVPA"
BASE COUNT          857 a         1532 c         1600 g          823 t
ORIGIN      
        1 gcgacgtttt cgggcagccg gatgatctcc agcgccctgg cgcgtttctc gagccgcttg
       61 aggaaacctc gctcctccag cgccgtgacg aggcggtgga tgcccgattt cgatttgagg
      121 tcgagcgcgt ccttcatctc gtcgaaggag gggggaacgc ccgtctcgcg cacccgctcg
      181 tgaatgaaca tcaaaagctc atgttgcttg cgcgtcagca tgtcagggct cccagaacgg
      241 cgttccggcg gctaccgaac aaatccgaaa cagccgcgaa tcgttctagt ttagttctcc
      301 ccaaccgtca acgctcaaga tgtgggagcg ctgttcccgt tggtggctct cgtcagtcga
      361 gcaacagtac ctcgacaggt tctcccgctt gtgcggctgc ggcatgcgga cgacgcacga
      421 tgagcgcgtc ggcggcggcg agagcgctct gcatcgaaga atcctggatc gtaaacggct
      481 cgacccatag ctcgccatcg cggcgtatga gacggccacg cagataatcc tggcgcgcat
      541 cgttggcggg aagcgcgccc gccagccgtg cctgttgcaa gggccgcgag aaggaaacgc
      601 ccagcaagcc ggcgatcagc ggctggagga acaacaccgc acagacgtaa ctcgacaccg
      661 ggttgccggg caggccgagc aatggctttt ctcccagctt cccgaagatc aggggctttc
      721 ctggccgcat cgcgatcctc cagaaatcga gggtgaaacc cttgggctcg agagcgcgct
      781 ggacgaggtc atgatcgccc accgacgcgc ctcccatggt cacgacaacg tcggcaggcg
      841 aagccaattc cggaatccgc tgcaaggctt cgatcgtatc gggaaagatt ccgagatcga
      901 gcggctggcc gccccagatc tcgatcagcg cggcgagcgc atatcccgac gaagcaacga
      961 ttccgcctgg gcgttgcggt gcatcgggtg gtgcgagttc gtcgcccgtt gcggcgaagg
     1021 cgatgacagg ccggcgttgc acatcgacaa tggaaatgcc gcccgccgcc agcagcgcca
     1081 gatcgcgcgc cgtgagtttg cgccctgccg cgcagagcac gtcacgcttt tgaaaatcga
     1141 aacgcgccgc gcggatgtgc cgtgccgcgg cgggcgcgcg gagcgtggcc cgatcgtcct
     1201 tcgcctccgt attctcctgg atgacgatcg cgtccgcacc ttccggaacg acaccgcccg
     1261 tgaagatacg tacggcttcg ccggcgcgca ccgacccggc aaagggatga ccggcgggtg
     1321 cggcgccgac gacgcgcaaa gttacgggga cggtatgcgt atcggcgagg cgcaccgcgt
     1381 agccatccat cgccgaaatg gggtgcggtg gctggtcgag cgcggcaatc gcatcggccg
     1441 agaggacgcg gcgatgcgcg attgcgagag ggacgcgttc gcttccaagt ggcgcgacgg
     1501 cagcgccaat gcggcgcacg gcgtcttcga cggaaatcac ctgtcccgcc gcggtgcagc
     1561 aaagctgccg cttttgccgc cggacttgga gagcagccgc accgattcga tgacagcgcc
     1621 gcgatcgatc gccttggtca tgtcgtagat ggtgagtgct gcgatcgcgg ccgccgtgag
     1681 cgcttccatt tcgacgccgg tttcggcctt cgttttcgtg gaagtgacga tccgcagcgc
     1741 gtggcgctcc gggagatgct cgatctccac ggccgcctgc gtcagagcaa tggggtggca
     1801 aagcgggata agttccgaag tcttcttggc ggccatgatc ccggcgagcc gcgcggccgc
     1861 gatcacgtcg cccttgggcg cggcgccccc gcagaccgcc gcgaatgcat ccgccgagag
     1921 cacgacgatc gcttccgcct gcgcctcgcg cacgctcgcc tctttcgcgg agacgtccac
     1981 catgcgcgcc gcgccatgct cgtcgagatg ggtaagtttg ctcatactgc cgatctatag
     2041 ctcgtttcgc tcaattcgaa aaattgcaga tgcatgagcc gggaatggtc acgcggagcc
     2101 gcggaggacg cggaggacgc ggaggacgcg gaggacgcgg aggacgcgga ggacgcggag
     2161 gacgcggagg acgcggagaa aaaactgaaa cgccaaggcg ccaagaaaag tatcgcgcgc
     2221 gttcggcgcg caatgctgag ccggctgcgc cgtctcacgc aggcggcatt gcgctacgtg
     2281 cgccttggcg tcttggcgcc ttggcggttc agcacttctc cgtctccgcg tgaacctttt
     2341 ttccgcaacg cggagtattc actatcgcgg agcagggcgc gggtcgctgc ggcgatgttt
     2401 ttttcgcgca tcaggctctc gccgatcaga aatgtggtga cgcccgcgtc ggcgagccgc
     2461 cgcagatccg acggcgagga cagtccgctt tcgccaatga caaggacgtg gcggggaatt
     2521 gccggagcaa gccgcaacgt cactgcggga tcagtgacga aagtcttcag gtcgcgattg
     2581 ttgatgccga tcatctccgc gccgagcgcc agcgcgcggt cgcgctcgat ttcgtcgtga
     2641 atctccacca gtgcgtccat cccccagtgc cgagccgcgg cgaggagcgc gcgcgcatca
     2701 tcgtcgtcga cggcggccag aatgatgaga atgcaatccg caccgagggc gcgcgattgc
     2761 acgatctggt agggatcgaa caagaaatct tttcgcaaca cgggaagcgt cacggcggcg
     2821 tgggcggttt caagatgcgc cggcgctccc tgaaacgatg gtccatcggt gaggacggaa
     2881 agacaggcgg cgccaccgtc gcgataggcg cgggcgattg tcccgggatc gaaatccgcg
     2941 cgaatgagac ccttcgatgg actcgcccgc ttgatttcgg caatgagtcc gtaataaccg
     3001 gcatcgcggc aggcggccag tgcggcgcgg aagccgcgcg gcggtccggc agttcgcgct
     3061 tgttcgtcga gcatcgattc gggcatttcg tgttttgcgc gcgcgatttc ctcgcgcttg
     3121 tagacggcga ttgcgtcgag aatggtcacg aggcgagcgc ctgtgtcgtt gtccgcagcc
     3181 gggcgagcat gtcgaaagcg ccgccgtccc gaattgcggc atcggcctga cacgcgcctt
     3241 cgctgatgtc cgccgccttg ccgccgacaa cgagcgccgc ggctgcgtta aggacaacaa
     3301 tatcccgata cgcgcaattt ccgtcgcctt gcagcatcgc ttcaagctcg tttgcgttga
     3361 acgccgcatc gccgcccttc acgtcttcgg gcgagactcg gcccaggccg acatcttcgg
     3421 gagtcagttg aaatcgcctg atcttgcctt gcgcgatctc gacgacgagg ctcgcatcgg
     3481 tggtcgtcag ctcgtccatg ccgtcccggc catgcacgat ccaggcgtgc tcggtcccga
     3541 gctgcaccag aacattggcg agcggctcga cccacctctc ggcgaaaacg cccagcacct
     3601 ggcggtgcac ctgtgccgga ttgctgatgg ggccgagcag gttgaaaacg gtccggaagc
     3661 ccagttcctt gcgtacgggc gccacgtgtt tcatcgccgg atgataggcg ggcgcgaaga
     3721 ggaagcagat gcccagccgg tcgagacagg tttgtgccgc ctgcggtgtg agatcgatgc
     3781 ggacgtggag cgcttccagc acgtcggcgg ctcccgcgcg cgacgacatg ttgcggttgc
     3841 cgtgcttggc aacgggcacg ccgcaggccg caaccacgaa agacgcggcc gtggaaatgt
     3901 tcaacgtccc gcgtccgtcg cctccggtgc cgcagagatc gatggcgccc gcgggcgcct
     3961 cgatcgtccg catcgcggcg cgcatcgcgc gcgcggcgcc tacgatctcg gcgaccgtcg
     4021 gctcgcgcag cgtcagagcc gtcaggaacg ccgccatgcg ggcttcggaa acttctcccg
     4081 ccatgatggc gccgaaggcg cgcagggacg attccgcact gagcgagcgg ccttcggcca
     4141 gccgcttgag cagggacggg aaatcctcgt cggcgctcat cgggtccgtt caggcgggca
     4201 cgcgctcgcg cgccagcgcg agaaaattgc gcaggagggc gtggccgttc tccgaggcga
     4261 tgctctccgg atggaactgc acgccatgca cgggatgttc cttgtgcatc acgcccatga
     4321 tcacgccgtc gctcgtaccc gcgttgacgg cgaggcaagt gggcacgctt tcgggatcga
     4381 ccgtgagcga atggtagcgc gtcgcctcga aatcattgcc gagaccgcgg aacacgccgc
     4441 tgccgtcgtg atggattcga ctaaccttgc cgtgcatggg aacgggcgcg cgcacgatgc
     4501 ttccgccgta agcctgggcg atcgcctggt ggccgagaca gactccgagg atcgggacgc
     4561 gaccgtcggc ggcacggatg aggccgaggc agataccggc cctgtcggga tcgcacgggc
     4621 ctggcgacag cacgatcgcc tccggtcgca actcgagggc gtcccgaacc gcaatctcgt
     4681 cattgcggcg gacatctacc tccgccccca attccgacag atagtggacg aggttgtaag
     4741 tgaaactgtc gtaattatcg atcaggagca gcatggcgca accgtaccga tttcacgctt
     4801 ccggcgccat tg
//
LOCUS       DASZMC010000535         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2137017,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000535 DASZMC010000000
VERSION     DASZMC010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137017"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1106
                     /locus_tag="VG274_10925"
     CDS             24..1106
                     /locus_tag="VG274_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWE07213.1"
                     /translation="MSLNKGFHSLRIAEVRRETADAVSIRFDVPGELREAFAFQAGQH
                     LTLKAEIGGEDVRRTYSVCVSPAENEIRIAIKQMPAGKFSAWANADLRADQRIDVLPP
                     MGRFVLPQSDETSPHYVALSGGSGITPVISILKTVLQTKPRSKFTLLYGNRDTASIMF
                     LEELAALKNRYIDRLEVYHFLENEAEDIELFNGRLDRAKCEEVLSTLVDVSATDAFFI
                     CGPGPMMDAAEAALLARGFPAGRIFIERFTTSALSAEQVARNEALQQKAAGTALLVTL
                     DGRRSRIRFDADKGNILESVQASGLPAPYACKSGVCTTCRAKILSGTVTMKKNYGLSD
                     AELSQGYVLTCQAVPTSSEVALSYDA"
     gene            complement(1729..3072)
                     /locus_tag="VG274_10930"
     CDS             complement(1729..3072)
                     /locus_tag="VG274_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWE07214.1"
                     /translation="MSAKRTLVVGAVAVAVLAAAAFYWIVLGPGPMDFAGGARVALSA
                     YHGNDPTGVPQEIRNASPVKRGEYLARAADCTACHTARGGTLYAGGLAFVLPFGTIYS
                     ANITSDKDTGIGNWSDAEFLNAIHKGVNRDGVPLYPAMPYASYTGMADADALAIKAYL
                     FSLAPVHRDVPHSKLIFPFNQRWLIGVWSFLFNPDKRFEPNPERSPQWNRGAYLAENL
                     AHCGECHTPRNLAFALDNRNKFAGATQQGWRAYNITSDSTSGVGAWSAGQLVQYLSLG
                     HADGRGSAAGPMGEAVDKSLKYLTADDTAAIVAYLQTVPAIASSDLPTPKAGPAPASH
                     KQGVIAGFDPRGKQIFEGACASCHEWTGVSFLTRYATLTGARAVNDPTAVNVAQIVLQ
                     GTMRDTPHGKVFMPSFGRAYSDSEVAAVANYVTARFGAKPSAVTVKDVAMMRRQD"
     gene            complement(3069..5255)
                     /locus_tag="VG274_10935"
     CDS             complement(3069..5255)
                     /locus_tag="VG274_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWE07215.1"
                     /translation="MTIQALSALSRRTVLQTAAAAGGGFLLGFHIPVAQAEVSNTAFG
                     VTPDAAPFAPNAFIRIDRVGKVTLIMPQVEMGQGVYTSLAMVLAEELDTDWNRVAVEH
                     APANEKLYANPMLSVQATGNSNSVRAFWLPMRKAAAQSRALLIAAAAKEWNVDPGACA
                     AANGSVIHKPSNRTLDYGALADRANGTPPTNPPLKTPKEFRLIGKPLKRLDTPGKVNG
                     ATLYGIDVMLPGMKFATIAASPTFGGKVGHVDDRAAKQIPGVQKVVVLDDMVAVVADH
                     MWAAKRGLDTLIVTWNAGPNGHVDSALIWKQIKSASNQSGLVAKKIGDADRALQSGDR
                     FEAHYELPFLAHATMEPMNCTVHLTQGACEVWLGTQIMARVQSTAAATAGVPLDRVIV
                     HNHMLGGGFGRRLEPDMVVKAIRVAQHVDGPVKVVWTREEDIQHDLYRPVYYDRLTAT
                     LKDGKIDGWHHRVTGSSVVARWLPPAYKANHNIDFDTVDSAADVPYDIPNLRVEVVRC
                     EPPAVPTGFWRGVGPNNNVFAIESFIDELAKRAGKDSVEFRRAMLTKTPRLLAALDLA
                     AEKAGWGSPLPRRHGRGVSCQVSFASFIATVAHVEVDDQGEVRLHRLTTAVDTGIAVN
                     PDTIIAQLQGGMIFGATAVLYGEITIRNGRVEQSNFNDYRMLRIDQAPAIDVHLIKSG
                     EPPGGIGETGVTTSSPAIRNAIYAATGIPLRRLPVDRDVLAGRKRA"
BASE COUNT         1010 a         1738 c         1769 g         1053 t
ORIGIN      
        1 gcttcaagtg ccattgagcc cgcatgtcgc ttaacaaggg cttccattcg ctgaggatcg
       61 ccgaggtgcg ccgcgagacg gcggatgcgg tttccattcg cttcgacgtc cccggcgaat
      121 tgcgcgaagc cttcgctttt caggcgggcc agcacctgac gctgaaagcg gaaatcggcg
      181 gcgaggacgt gcggcgtacc tattcggtct gcgtctcgcc ggccgaaaac gaaatccgca
      241 tcgcgatcaa gcagatgccg gccggaaagt tctcggcatg ggcgaacgcg gacctgcgcg
      301 ccgaccagcg gattgacgtg ctgccgccga tgggccgctt cgttctgccg caaagtgacg
      361 aaacgtcgcc acattacgtc gcgctttccg gcggctccgg cattacgccg gtcatctcca
      421 tcctcaagac cgtgctgcag acgaaaccgc ggagcaagtt cacgctgctg tacggcaatc
      481 gcgacacggc ttcgatcatg tttctggagg agctggcggc gctgaagaac cgctatatcg
      541 atcgtctcga agtgtatcac ttcctcgaaa acgaagcgga ggacatcgag ctattcaacg
      601 gccggctgga ccgcgccaaa tgcgaggagg ttctgtccac gcttgtcgat gtgagtgcga
      661 cggatgcatt cttcatctgc ggccctggtc cgatgatgga tgccgccgaa gccgctctcc
      721 tggcgcgcgg tttcccggct gggagaatct tcatcgagcg cttcaccacc agcgctctct
      781 cagccgaaca ggttgcgcgc aacgaggcgc tgcaacagaa ggccgcgggt accgctctcc
      841 ttgtcacgct cgacggccgc cgctcgcgca tccgtttcga cgcagacaag ggcaatattc
      901 tcgagagcgt gcaggcgtcg ggccttcccg cgccctacgc gtgcaaaagc ggcgtgtgca
      961 cgacgtgccg ggcgaagatt ctgtcgggca cggtgacgat gaaaaagaac tatgggttga
     1021 gcgatgcgga actgtcacag ggctacgtgc tgacctgcca ggccgttccc accagcagcg
     1081 aggtcgcact cagttacgat gcgtgaagcg tgcttcgatc agagtgaatc gccatctgga
     1141 ctggcgtttt cctcccccgc caagggcggg ggagggggac cgcgaagcgg tggagggggc
     1201 actcgcgcac gaggatgcgg caggcgtgca tcgatcaaat caagcgccgc agaaagcgag
     1261 cgatagacgc ttcccggtgg gagtcgtgcc agcgctcggc gtaactgcca gccgcgcgct
     1321 ggctgcgaag gttgggattt tgtgcgcgct gcgtaacaaa gtctggcgca caacgcgcac
     1381 gtcgctcgcg cgccccctcc accgcttcgc ggtccccctc ccccgcgcat tcgcgcgggg
     1441 gaggaaagaa caggtgtaca cgatttgtag acgcgctagc gctttcgcgg gctgacatgt
     1501 ttggtttaat atgaacgaaa cattttaacg caaagtacgc ggaggacgca gaggagtttg
     1561 ttgcacgctt cctcctacgc tgctgcgcag cttcggagga catcctcgct cgcccccgaa
     1621 gcttttgcgt aggcgcttcg cgcgccgtcg tcttcctgcg tccgctgcgc ttcctcggcg
     1681 ccctctgcgt taaatctgtg tttgcgtcac tcgtcgaggt tccgcgacct agtcttgtct
     1741 ccgcatcatc gcgacgtcct tgaccgtgac ggccgagggt ttggctccga accgggcggt
     1801 gacgtaattg gcgacggccg cgacttcgga atccgaatag gcgcggccga acgacggcat
     1861 gaagacttta ccatgcggcg tgtcgcgcat cgtgccttgc aggacgattt gcgccacgtt
     1921 gacggcggtc ggatcgttga ctgcgcgcgc gccggtcagc gtggcataac gcgtgaggaa
     1981 actgacgccc gtccattcgt gacagctcgc gcaggcgcct tcaaagatct gcttgccgcg
     2041 cgggtcgaag ccggcaatca cgccttgctt gtgcgacgcg ggcgcgggcc cagcctttgg
     2101 cgtgggcaaa tcggacgagg cgatcgccgg cacggtctgc agataggcga cgatggcagc
     2161 cgtgtcgtcc gcagtcagat atttgaggct cttgtccacg gcctcgccca tcggaccggc
     2221 ggccgacccg cgtccgtctg catggccgag cgagagatat tgcacgagct gacccgcgct
     2281 ccacgcgcca acaccggagg tggagtcgct cgtgatgttg taggcgcgcc agccctgctg
     2341 ggtcgcgccg gcaaatttgt ttcggttgtc cagcgcgaat gccagattgc gcggcgtatg
     2401 acattcgccg caatgcgcca gattctcggc cagataggcg ccgcggttcc attgagggct
     2461 gcgctccgga ttgggttcaa agcgcttgtc cggattgaac aaaaacgacc atacgccgat
     2521 cagccagcgc tggttgaacg gaaaaatcag cttgctgtgc ggcacgtcgc gatgcacggg
     2581 tgcgagactg aacagatacg ccttgatcgc cagcgcgtcg gcgtcggcca tgccggtata
     2641 cgaagcgtag ggcatcgccg gatagagagg gacgccgtcc ctgttgacgc ccttgtggat
     2701 ggcgttcagg aattcggcgt cgctccaatt gccgatgccc gtatccttgt ccgacgtgat
     2761 gttcgccgag tagatcgtcc cgaacggcag aacgaaagcg agaccgcccg catagagcgt
     2821 gccgcccctg gcggtatggc aggccgtgca atccgcggcg cgcgccagat attccccgcg
     2881 cttgacagga cttgcgttcc ggatctcctg cggaacgcca gtgggatcgt ttccatgata
     2941 ggcagaaagg gccacacgtg cgccgcccgc aaaatccatc gggccgggcc caagaacgat
     3001 ccagtaaaac gcggccgcgg cgagaacggc aaccgctact gcgccgacga cgagcgtgcg
     3061 ctttgcgctc atgcccgttt cctcccggcg agaacgtcgc gatcgacggg aaggcgccga
     3121 agcggaatgc ctgtcgcggc atagatcgcg ttgcggatgg ccggcgacga cgtggtcacg
     3181 cccgtttcgc cgatgcctcc gggcggctcg ccgctcttga tcaggtgcac gtcgatggcg
     3241 ggggcctgat cgatgcgcag catgcggtaa tcgttgaaat tggattgctc cacgcggcca
     3301 ttcctgatcg tgatctcgcc atagagcacg gccgtcgcgc cgaagatcat gcccccctgc
     3361 agctgggcaa tgatcgtgtc cggattgacg gcgatacctg tatccacagc cgtcgtgagg
     3421 cgatgcaggc gcacttcgcc ctgatcgtcc acctccacat gggcgacggt tgcgatgaag
     3481 ctggcgaacg aaacctggca cgacacgccg cgtccatgac gccgcggaag cggcgaaccc
     3541 cagcccgctt tctccgcggc aagatcgagc gcggcgagca gacgcggagt tttggtgagc
     3601 atcgcgcggc ggaattcgac cgaatccttg ccggctcgct tcgccagttc atcaatgaag
     3661 ctctcgatgg caaaaacatt gttgttgggg ccgacgccgc gccagaagcc ggtgggcacc
     3721 gccggcggtt cgcagcgcac cacttccacg cgcagattgg ggatgtcgta aggaacgtcg
     3781 gccgcgctgt cgaccgtgtc gaaatcgatg ttgtgattgg ccttgtacgc gggcggcagc
     3841 cagcgcgcga ccaccgacga gccggtgacg cggtgatgcc agccgtcgat cttgccgtcc
     3901 ttcagcgttg cggtgagacg gtcgtaataa acgggacggt agagatcgtg ctggatatcc
     3961 tcctcccgcg tccacacgac cttgaccgga ccgtcaacat gctgggccac gcggatggcc
     4021 ttcacgacca tgtcaggctc gagccggcgg ccgaagccgc ccccgagcat gtgattgtga
     4081 acgatcaccc tgtcgagcgg aacgccggcg gtcgccgcag ccgtcgattg cacgcgcgcc
     4141 atgatctgcg tgccgagcca gacttcgcag gcgccttgcg tcaggtgaac cgtgcagttc
     4201 atcggctcca tggtcgcgtg cgccaggaac ggcaattcgt aatgcgcttc gaatctgtcg
     4261 ccgctttgga gcgccctgtc ggcatcgccg atcttcttcg caacgagtcc actctgattg
     4321 ctggcggatt tgatctgttt ccagatcagt gctgaatcga catgcccgtt cggccctgca
     4381 ttccaggtga cgatcagcgt gtcgagcccg cgtttcgccg cccacatgtg atctgcgacg
     4441 accgccacca tatcgtccag caccacgacc ttctgcacac cgggaatttg cttcgcagct
     4501 cggtcatcga cgtgaccaac cttgccgccg aaggtcggac tggcggcgat ggtcgcgaac
     4561 ttcatgccgg gcagcatcac atcgatgccg tagagcgtcg cgccattgac cttgccaggc
     4621 gtatcgaggc gcttcaacgg tttgccgatc agccgaaatt ctttgggcgt tttcagcggc
     4681 ggattggtgg gcggcgtacc gttcgctctg tcagccaacg caccgtaatc gagcgtccga
     4741 ttgctcggtt tgtgaatcac cgaaccgttc gcggcggcgc aggcgccggg gtcaacgttc
     4801 cactctttcg ccgcagcggc aatcagcagt gcgcgcgact gcgcggcagc cttgcgcatc
     4861 ggcagccaaa aggcacggac ggaattggaa ttgccggtcg cctgcacgga gagcatcgga
     4921 ttggcgtaaa gtttctcgtt ggcgggcgca tgctccaccg cgacacggtt ccaatccgta
     4981 tcgagctctt cggcgaggac catggcgagg gaggtataaa cgccctgccc catctcgacc
     5041 tgcggcatga tgagcgtgac cttgccgacg cgatcgatgc ggatgaacgc gttgggggca
     5101 aacggagcgg catccggcgt gacgccgaat gccgtgttcg aaacttccgc ctgcgcgacc
     5161 ggaatatgaa aaccgagcag gaaaccgccg cccgccgcgg cagctgtttg cagcaccgtg
     5221 cgtcgtgaca gggccgatag tgcttgaatg gtcatgaacc ctcccgctat tccggcaatc
     5281 agtggtccac ttctgagaat cgaattccaa aataattgtc atggccccgc tcgacggggc
     5341 aacccagcca gcgcgcgtcc gcgcgctgaa taaacgctcg gagcggtctc gtccgggtgg
     5401 cccgctggag tttacactcc ggccgcgctc tcgcgcagac cgggttggca ggccatgaca
     5461 gtttttttgg caggaagtgt cggattcgga ccactaaacc tgctccgtgc ctgccgcctt
     5521 cttgatcgct tcgcgaatgc gcacataagt gccgcagcgg cagatgttgc
//
LOCUS       DASZMC010000536         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_221122,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000536 DASZMC010000000
VERSION     DASZMC010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221122"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VG274_10940"
     CDS             <1..1180
                     /locus_tag="VG274_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWE07216.1"
                     /translation="STAVLAMANLAMVTGNIGRPGVGVNPLRGQNNVQGSNDVGSFPH
                     ELTGYRHVSDPALRADFEREWHVTLDAEPGLRIPNMFDAALDGSFKGLYIEGEDIAQS
                     DPNSQHVASALQAMEIVIVQDIFLNETARYAHVFLPGSSFLEKDGTFTNAERRISMVR
                     KVIPPLAGKEDWEVTCALSNALGYPMNYAHPSEIMDEIARLTPTFAGVSYEKLDRLGS
                     VQWPCNDDAPEGTPVMHAGQFVHGKGRFMPTEYVPTDERTSARYPLILTTGRILTQYN
                     VGTQTRRTRNVAWHAEDRLEIHPHDAEQRGVRDGDWVEVSSRAGAIALRAEITERVQP
                     GVVYTTFHHPETGANVVTTENSDWATNCPEYKVTAVQVMPTSRRPERLTADRMMHAAK
                     "
     gene            1209..1445
                     /locus_tag="VG274_10945"
     CDS             1209..1445
                     /locus_tag="VG274_10945"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HWE07217.1"
                     /translation="MEIHHLARMANEIGGFFEQMPDHAEAVNSISAHLKNFWEPRMRR
                     QIIEYAREGGAELTAIVREAVLTLEPPGAPIHHG"
     gene            1449..2309
                     /gene="fdhD"
                     /locus_tag="VG274_10950"
     CDS             1449..2309
                     /gene="fdhD"
                     /locus_tag="VG274_10950"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWE07218.1"
                     /translation="MPEPPKTVSEFVKDHPALKWRGGAAAREPDHLAVEEPLEIRLAG
                     RRFTLTMRTPGHDEELAAGFLLAEGFIASREELGEIRRVRGAKGLPEPNAIDVVLNVP
                     AAGMRERLKRNFTVTSSCGVCGKTSIEALERRIAPLNDGPEVAARTLFRLPAKMREAQ
                     RVFTATGGLHAAALFRIAGTSDAPGEGARMLVLREDVGRHNAVDKAIGHALTHHMIPL
                     ADSIMMVSGRLSFEIVQKAAAAGIPILCAVSAPSSLAVELAEEVGMTLVGFLREPNFN
                     IYTRQERILA"
     gene            2306..2806
                     /locus_tag="VG274_10955"
     CDS             2306..2806
                     /locus_tag="VG274_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07219.1"
                     /translation="MTGRRVATHHRYVLWLIAVLLPAAGCVSPPRDFPPWMRVTRVEV
                     LKTDTPRGDVVKEIRDPGQVARIVAFANANLSGYREPWFGEPLPAIDVAFYDRRRYVG
                     HFGVGLEFFEIHRGSITFMSRPASEQQVHEIMQLINVDDDVLGSVPDEELPDSDCGSP
                     LIGECP"
     gene            2803..>3602
                     /locus_tag="VG274_10960"
     CDS             2803..>3602
                     /locus_tag="VG274_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWE07220.1"
                     /translation="MKKNESDAPSKPDSARVVRTLAVDIGGTGIKVMVLGEAGTPLTE
                     RLREPTPAKATPKAVIGVVRELAKQQGEFDRVSVGFPGVVKDGVVYSAPNLGKGWDGF
                     NLREALEKKLDHPVRVANDADVQGLGSVTGRGLELVVTLGTGFGSVLFANGTRIHLEL
                     GHHPFHKGKTYEDELGVRALKKKGVKKWNAMFREALGDLKRTFNYDRLYIGGGNAKAI
                     NFKLPDSVKIVSNTEGLLGGIKLWSEPDTSSVAEDTAKVPDSGQADAAA"
BASE COUNT          709 a         1072 c         1195 g          626 t
ORIGIN      
        1 cagcaccgcg gtgctcgcga tggcgaacct tgcgatggtg actgggaata tcgggcggcc
       61 gggggtggga gtgaatcccc tgcgcgggca gaacaacgtg caaggctcga acgacgtcgg
      121 ctcgttcccg cacgagttga ccggctatcg ccacgtctcg gaccctgctc tgcgcgcgga
      181 cttcgagcgc gaatggcacg tcacgctcga cgccgagccg ggtctcagga tcccgaacat
      241 gttcgacgcc gcgctcgacg gcagcttcaa ggggctctac atcgaaggcg aggatatcgc
      301 gcagtcggat ccgaattcgc agcacgtcgc ctcggcgttg caagcgatgg aaatcgtgat
      361 cgtgcaggac atcttcctga acgaaaccgc caggtacgcg cacgtgttcc tgcccggctc
      421 gtcgttcctc gagaaggacg gcacgttcac caacgccgag cggcgcatct cgatggtgcg
      481 gaaagtaata ccgccgctgg cgggcaagga agattgggaa gtgacctgcg cgctgtcgaa
      541 cgcgctaggc tatccgatga actatgcgca tccgtcggaa atcatggacg agatcgcgcg
      601 cctgacaccg accttcgccg gcgtcagcta cgaaaagctc gaccgcctcg gcagcgtgca
      661 atggccttgc aacgacgacg cccccgaggg cacacctgtc atgcatgccg gccagttcgt
      721 gcatggcaag ggccggttca tgccgaccga atatgtgccg acggacgagc gcaccagcgc
      781 gcgctatccg ctgattctca cgaccgggcg catcctcacg caatacaacg tcggcacgca
      841 gacgcggcgc acccgcaacg tcgcatggca cgccgaggat cggctggaaa ttcatccgca
      901 tgacgccgaa cagcgggggg tgcgcgacgg tgactgggtg gaagtgtcga gccgggcggg
      961 cgcgatcgcg ctgcgcgcgg aaatcacgga aagggtgcag ccgggcgtcg tgtacacgac
     1021 gttccatcat ccggagacgg gcgccaacgt cgtcacgacg gaaaattccg actgggcgac
     1081 gaattgtccc gaatacaagg tgacggcggt gcaggtgatg cccacatcgc gccggcccga
     1141 acggctgacc gccgatcgaa tgatgcacgc ggcgaaataa ttgcgcgccg ttccggagcc
     1201 actcccgaat ggagattcat cacctggcca ggatggccaa cgagatcggc gggttcttcg
     1261 agcagatgcc cgatcatgcg gaggcggtta actcgatctc ggcgcatctg aagaatttct
     1321 gggagccgcg gatgcgccga cagataatcg agtatgcgcg cgagggcggc gccgaactca
     1381 ccgccatcgt ccgtgaagcc gtcctcacgc tggaacctcc gggcgcgcca atccatcacg
     1441 gctgaaccat gccagaaccg ccaaagaccg tgagcgaatt cgtcaaggat catccggcgc
     1501 tcaaatggcg cggtggcgcg gccgcgcgcg aacccgacca tctcgcggtt gaggagccgc
     1561 ttgaaatcag gctggccggg cgccgattca cgctcacgat gcgcacgccg ggccatgacg
     1621 aggagctggc ggcgggcttc ctgctggcgg aaggattcat cgcatcgcgc gaagaattgg
     1681 gtgagattcg ccgcgtgcgc ggcgcgaaag gattgcccga gccgaacgcg attgatgtcg
     1741 ttctcaatgt gcccgctgcc ggaatgcgcg agcggctaaa gcgcaatttc accgtcacgt
     1801 cgagttgcgg agtatgcggc aagaccagca tcgaggcgct cgaacggcgt atcgcgccgc
     1861 tgaatgacgg ccctgaagtc gcggcgcgca ctcttttccg gctgcccgcg aaaatgcgtg
     1921 aagcgcagcg ggttttcacc gcgaccggcg ggcttcacgc ggcggcattg ttcagaatcg
     1981 ccggaacttc cgatgcgccg ggagaaggtg cgcggatgct tgtcctgcgc gaggacgtcg
     2041 gacgtcataa cgcggtggac aaggcgatcg gccacgcgct cacgcatcac atgattccgc
     2101 tcgcggattc aatcatgatg gtcagcgggc ggctgagctt cgagattgtg caaaaggcgg
     2161 cagccgccgg cattccgatt ttgtgcgcgg tctccgcgcc gtcatcgttg gccgtggaac
     2221 tggcggagga ggtcgggatg acgctggtcg gatttcttcg cgagccgaat ttcaatatat
     2281 acacacgcca ggaaaggatc cttgcgtgac aggccgccgc gttgcgactc accatcggta
     2341 tgttctctgg ctcatcgcgg tcctgcttcc agccgccgga tgcgtttcgc cgccgcgcga
     2401 ttttccgccg tggatgcggg tgactcgcgt cgaggttctc aaaaccgata ctccgcgcgg
     2461 cgatgtggtc aaggagattc gcgatcccgg acaggtcgcg cgcatcgtcg cgttcgccaa
     2521 cgcgaacctg agcggttatc gcgagccgtg gttcggagag ccgcttccgg cgatcgacgt
     2581 cgcgttctac gaccggcgcc gttacgtcgg ccatttcggc gttgggctgg agttctttga
     2641 aatccatcgc gggagtataa ccttcatgtc gcggcctgca tccgagcaac aggtccacga
     2701 gatcatgcaa ctgatcaacg tggacgatga tgtcctaggt agcgttcctg acgaggagct
     2761 gccggattcg gattgcggct cgccgctcat aggggaatgc ccatgaagaa aaatgagtcc
     2821 gacgctccgt caaagccgga ttcggcccgc gtggtccgca cactggcggt cgatatcggc
     2881 ggcaccggca tcaaggtgat ggtcctcggc gaggcgggca cgccgctgac cgagcgattg
     2941 cgcgagccga ctcccgcgaa ggcgacgcct aaggcggtaa tcggcgtagt tcgcgagttg
     3001 gcgaagcagc agggcgagtt cgatcgcgtg tcagtgggct ttcccggcgt ggtcaaggac
     3061 ggggtcgttt attcagcgcc gaacctcggc aagggatggg acggcttcaa tctgcgggag
     3121 gcattggaaa aaaagctgga ccatccggtg cgcgtcgcca atgacgccga tgtgcaggga
     3181 ctcggcagcg tgaccggccg cgggcttgaa ctggtggtga cgttggggac cggctttgga
     3241 tcggtgctat tcgccaacgg cacgcgcatc catctggaac tcgggcatca tccgtttcac
     3301 aagggcaaga cctacgaaga tgaattgggc gttcgcgcgc tgaagaaaaa aggcgtcaag
     3361 aagtggaacg ctatgtttcg cgaggcgctc ggcgatctca agcggacgtt caattacgac
     3421 cgcctgtata tcggcggcgg caacgcgaaa gcgatcaatt tcaagctgcc cgacagcgtc
     3481 aagatcgtta gcaacactga aggattgctc ggcggcatca agttgtggag cgagcccgat
     3541 acatcttcgg tcgcggaaga taccgccaag gttccggatt cgggacaggc ggacgcggcg
     3601 gc
//
LOCUS       DASZMC010000537         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1034318,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000537 DASZMC010000000
VERSION     DASZMC010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034318"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VG274_10965"
     CDS             <1..833
                     /locus_tag="VG274_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HWE07221.1"
                     /translation="DPGTILAAVTPVPDTISEYQFAGLLRGARVELADCVSQPLKVPA
                     EAEIVLEGEVSLTDYRDEGPFGDHTGYYNSVEQFPVFTVTAITMRREPIYLSTYTGRP
                     PDEPSILGEALNEVFIPLLQQQFPEIVDFWLPPEGCSYRIAVVSMRKAYPGHAKRVMM
                     AVWSYLRQFMYTKWVVVVDDDIDARNWKDVMWAVATRMDPARDITVVENTPIDYLDFA
                     SPDSGLGSKIGLDATNKLPPETKRDWGRLIRMDDEVIRTVTEKWPRLGLPGTGKPIWR
                     "
     gene            892..1947
                     /locus_tag="VG274_10970"
     CDS             892..1947
                     /locus_tag="VG274_10970"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HWE07222.1"
                     /translation="MRAAWQSAGICLIAFAAPATPAAAQACGPLKIVASVDLKAAPND
                     AAVFVPVSLRQQTKYMLLDTGGTISELTPAAVSELGLESVKTPTLRFYDVRGNYVDHH
                     TIVPDFAIGGLTGHNVDFVVGPNDLFRDSSDVAGILGPGILRYYDVALDIAGRKLTLL
                     SQDHCDGKVVYWKADSVAVVPMQVAKASGHIVVSVTIDGQSLSANLDTGASRSTLNLS
                     VAEGDFHLNEKQPDMIPVGRMRDKDDSIVYRHAFKSLSFEGLTISNPSLDIIPDLERG
                     QMERPPATGTRMPDSSVEQKLPDLLIGMDILRHLHLYIAYGEQKLYITPAAEPAAADA
                     SSPASAAARPNDTAATH"
     gene            2023..2457
                     /locus_tag="VG274_10975"
     CDS             2023..2457
                     /locus_tag="VG274_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HWE07223.1"
                     /translation="MLLFESSIYAFGFYAALNALIMLVLSVRVVRARVKTQTPIGDGG
                     IPKMAGALRAHANNAEYVPMALILLWALASPLGRSIWLIHGMGVPLTIGRVLHAIALT
                     RTTGPSTLRVLGMALTWIAYIVGIAGVLWFVFLPQPNPLLTP"
     gene            2454..3797
                     /locus_tag="VG274_10980"
     CDS             2454..3797
                     /locus_tag="VG274_10980"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWE07224.1"
                     /translation="MTENRESREILEKLIRVALRAGADAADAILASNVSASVSYRLGR
                     LEELERAESRDLGLRVFAGAKVAFVSSTDISPGTLSELPERALAMARLAPEDRFASLA
                     PADRLATSFPTLDLEDTEEPSADVLIERAKTVEGAAMAIAGVTNSEGGGASFGRSEIA
                     LATSAGFFGCYAGTSHGIGVAVLAGDGTSMERDYDGVSARHVADLEPAESIGRRAGER
                     TVARLNPRKAKSQTVPVVFEPRVSSGLLGHFAGAISGASIARGVSFLKDNLGAEVFTR
                     GITIVDDPHRPRGLRSKPFDGEGVRNARMSLVVDGQLKTWILDCASARQLGLSTTGHA
                     ARGTGGPPSPATTNLYMEAGKRPPGDLIADIVQGFYVTELMGMGVNQVTGDYSRGASG
                     FWIENGAIAYPVAGVTIAGNLKDMFRRLTPADDLVFRYGTNAPTLRIEGMTVAGA"
     gene            3784..>4026
                     /locus_tag="VG274_10985"
     CDS             3784..>4026
                     /locus_tag="VG274_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWE07225.1"
                     /translation="MRERDAADLLVVENAVRKAGEIARHYFGGEYRRWSKSRGEPVTE
                     ADLAIDKFLHATLERERPDYGWLSEETGRDPANADAS"
BASE COUNT          757 a         1255 c         1264 g          750 t
ORIGIN      
        1 cagatccagg aacgattctc gccgcggtca cgcccgtacc cgacacgatt tccgaatacc
       61 agttcgcggg attgctgcgc ggcgcgcgag tcgaactggc cgattgcgta agccaaccgc
      121 tcaaagtgcc agccgaagcc gagatcgtcc tcgaaggcga agtgtcgctg accgattatc
      181 gcgacgaagg accgttcggc gatcacaccg gctactacaa ttcggtcgag caattcccgg
      241 tgttcaccgt gacggcgatc accatgcgcc gcgagccgat ctatctttcg acctacaccg
      301 gacggccgcc cgacgaaccg tcgattcttg gcgaagcgtt gaacgaagtg ttcattccgt
      361 tgctgcagca gcagttcccg gaaatcgtcg atttctggct gccgcccgaa ggctgctcct
      421 accgcatcgc ggtagtgagc atgcgcaaag cctatccggg ccacgccaag cgcgtgatga
      481 tggcagtgtg gtcgtatctg cggcagttca tgtacacgaa atgggttgtt gtcgtggacg
      541 acgacatcga cgcgcgcaac tggaaggacg tgatgtgggc cgttgcaacg cgcatggacc
      601 ccgcccgcga catcaccgtc gtcgaaaaca cgcccatcga ctatctcgat tttgcgtcgc
      661 cggattcggg gctgggttca aagatcggtc tcgacgcaac gaataagctt cctcccgaga
      721 cgaagcgcga ctggggccgt ctcatccgca tggacgacga agtgatccgc accgtcaccg
      781 aaaaatggcc ccgccttggc ctgccgggca cgggcaaacc gatttggcgt tagcggcttg
      841 gcgttgccgt gtctcgctgg taagaactcg ctgtcgaatc ccggggctga aatgcgcgcc
      901 gcgtggcaaa gtgcagggat ctgtctcatt gcgttcgcgg cgcctgcaac gcccgcggcc
      961 gcacaggcgt gcgggccgct gaagatcgtc gcgtccgtcg atctcaaggc ggccccgaac
     1021 gatgcggccg tatttgtacc ggtctcgctc cggcagcaga cgaaatacat gctgctcgat
     1081 accggcggca ccatcagcga actcacgccc gcagcggtat cggaacttgg gctcgaatcg
     1141 gtcaagacgc caacccttcg cttctatgac gttcgcggca actacgtcga ccaccacaca
     1201 attgttcctg atttcgcgat cggcggcctg actggccaca acgtcgactt cgtcgtcggc
     1261 ccaaatgacc ttttcaggga cagcagcgac gttgcaggga tcttggggcc gggtattttg
     1321 cgctactacg acgtcgcact cgacatcgcc ggacgaaagc tcacgctcct atcgcaggac
     1381 cattgcgacg gcaaggtggt gtattggaaa gcggattcgg tcgcggtcgt acctatgcag
     1441 gtcgcaaagg ccagtggtca tattgtggta tccgtgacga ttgatgggca atcgctgagt
     1501 gcgaatctcg atacgggcgc atcgaggagc acactcaatc tcagcgtcgc cgaaggagat
     1561 ttccacctca acgaaaagca gcccgacatg atccccgtcg ggcgcatgag agacaaggac
     1621 gactcgattg tgtaccggca cgctttcaag tctttgagct tcgagggtct caccatttcc
     1681 aatccctcgc tcgacatcat ccccgatctg gagcgcgggc agatggaacg gccaccggcc
     1741 accggaacgc gaatgccgga ctcgagcgtc gaacagaagc tgccggatct tctgataggc
     1801 atggacatcc tgcgccatct gcatctctac atcgcttacg gggagcagaa gctctacatc
     1861 acgccggcag cggagcccgc ggcagccgac gcaagttcgc ccgcttcggc ggcagcacgc
     1921 ccgaatgaca cagcggcgac gcattaatga ccgcggcttg accgcacgcg gactccactt
     1981 gcacattgcg ccgcgcccgg ccggggcggt caggggaaaa tcatgttgct gtttgaatcg
     2041 tcgatctatg cctttggttt ctacgcggcg ctgaacgcgc tcatcatgct cgtcctctcg
     2101 gtccgtgtgg tgcgcgcgcg ggtcaagacg caaacgccga tcggcgacgg cggcataccg
     2161 aagatggccg gagcgctgcg cgcgcacgcc aacaatgccg aatacgttcc gatggccctg
     2221 atcttgctat gggcgctcgc ttcgccgctc ggccgttcga tctggctcat ccatggcatg
     2281 ggcgttcccc tcaccattgg gcgggtgttg cacgcgatag cgctcactcg aaccaccggc
     2341 ccatcgacac tgcgcgtcct gggcatggcg ctgacctgga ttgcctacat cgtggggatc
     2401 gcgggcgtgt tgtggttcgt ttttctgccg caaccgaacc cgcttctcac tccgtgacgg
     2461 aaaatcgcga atccagggag atcctcgaga aactcatccg tgttgcgttg agggcggggg
     2521 ccgacgcggc cgatgcgatc cttgcttcca acgtatcggc aagcgtgtcc taccggctcg
     2581 gccggctcga ggagcttgag cgcgccgagt cccgcgacct tggtctgcgt gtttttgcgg
     2641 gcgcaaaggt ggcgtttgta tcttcgacgg atatttcgcc cggcacattg agcgaactgc
     2701 cggaacgggc gcttgcgatg gcgaggctcg cgccggaaga cagatttgcc tcgcttgcac
     2761 cggccgaccg gcttgccaca agctttccga cactcgacct cgaagacacg gaggagcctt
     2821 cggcggatgt cctgatcgag cgggcgaaaa ccgtcgaggg cgcggcaatg gcgattgcgg
     2881 gtgtcaccaa ttctgaaggc ggtggcgcaa gtttcggccg cagcgagatc gctttggcga
     2941 ccagcgcggg attcttcggc tgctatgccg gtacgtctca cggcatcggg gtcgctgtgc
     3001 tggccggtga cggcacctcg atggaacgcg attatgacgg cgtcagcgcg cggcatgtgg
     3061 ccgacctcga accggccgaa tcaatcggcc gccgcgccgg cgagcgcacg gttgcacgcc
     3121 tcaacccgcg caaggcgaag tcgcagaccg taccggtcgt gttcgagcca cgcgtttcct
     3181 ccggcctgct cgggcatttc gcaggcgcca tctcgggtgc gtcgatcgcg cgaggcgtca
     3241 gcttcctcaa agacaatctg ggggcggaag tgttcacccg tggaatcaca atcgtcgacg
     3301 atccgcatcg gccgcgcggc cttcgctcca agcccttcga cggtgaaggg gtgcgcaacg
     3361 cccgcatgtc gctggtcgta gacggccagc tcaagacctg gattctcgac tgcgccagcg
     3421 ccaggcagct cggcctgtcg acgaccggtc atgcggcgcg cggaacgggc ggcccgccct
     3481 cgcccgcgac gaccaatctc tacatggagg ccggaaagcg gccgcccggt gacctgatcg
     3541 cagacatcgt ccaggggttc tacgtgaccg agctgatggg aatgggagtc aatcaggtga
     3601 ccggcgacta cagccgcggg gcctcgggct tctggatcga aaatggcgca atcgcctatc
     3661 ctgttgcagg cgttacgatt gccggaaatc tcaaggacat gttccgccgc ctgacgccgg
     3721 cagacgacct tgtgttccgt tacggcacga acgcaccgac attgcgcatc gagggcatga
     3781 ccgttgcggg agcgtgacgc agccgatctc ctggttgtcg agaatgcggt gcgcaaggcg
     3841 ggagagatcg cgcggcacta ttttggcggc gaataccggc gctggagcaa gagccggggc
     3901 gaaccggtta cggaagccga ccttgccatc gacaaatttc tgcacgccac attggagcgc
     3961 gagcggcccg attatggctg gttgtcggag gagaccggcc gagatccagc gaacgcggac
     4021 gcttcg
//
LOCUS       DASZMC010000538         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2137660,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000538 DASZMC010000000
VERSION     DASZMC010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137660"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="VG274_10990"
     CDS             <1..1370
                     /locus_tag="VG274_10990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWE07226.1"
                     /translation="FKVRARGLKTVYVPHAIVYHYEGASSGTSVASGAKRFQEVNRPK
                     FKRKWSSAFAGNGQEGVNVELERDRGPRFRALMLDFEVPRIGFDAGSYAGIQEIRALQ
                     ALGFKVTFLPTNCAYLGRHTEALQRIGVETSYAPFVRSVEAFLAAHGREFELVYVTRH
                     QVAAQALAAIRKYAPQAKVVLNLADLHFLRELRAGVAAQDASRIDAAVRIRDSELAVL
                     RTVDLALSYSAVEEAVILSHNLNSTKTGRLPWIAEPIVELPGFAERRNIGFLGGFRHT
                     PNAEAVDFFLDRVMPGLIRELPDVQFEIYGSAISRENRKRWTRENAVVKGRVDRVEEM
                     LGHIRVFVAPLASGAGVKGKVFDALAHGVPAVLSPLAAEGIGVRDGTEVLIAWAPEEW
                     VKQIVRLYSDEKLWRHLSAAGRRLIETDYSFARGVETMRVALDKAGFYCEPDPGYLVL
                     NRCLP"
     gene            1410..>1641
                     /locus_tag="VG274_10995"
     CDS             1410..>1641
                     /locus_tag="VG274_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07227.1"
                     /translation="MTIVNTTYKFIYVHIPKTAGTAVKKYLGRFTRFCDVEVGGARDA
                     EGVAEYYRKRFRLAKHSFAREIKEAVGVEAFQH"
BASE COUNT          296 a          497 c          539 g          309 t
ORIGIN      
        1 ccttcaaggt acgcgcacgc ggactgaaga ccgtctacgt cccgcacgcg attgtctatc
       61 actacgaggg cgcatcatcg ggcacctcgg tcgcttcggg agcaaagcgc tttcaggagg
      121 tcaatcggcc caaattcaag cgcaaatggt cgagcgcctt cgcgggcaat ggccaggaag
      181 gcgtgaatgt agagcttgaa agagaccgcg gaccgcgatt tcgcgcgttg atgctcgatt
      241 tcgaggtgcc gcgcatcggc tttgacgcag gcagctatgc cggcatccag gagatacgcg
      301 ctttgcaggc gctggggttc aaagtgacgt tccttccgac gaactgcgcc tatctcggac
      361 gccacaccga agcgctgcag cgcatcggtg tggagacgtc ctatgcgccc ttcgtgcggt
      421 cggtcgaagc cttcctggcc gcgcacgggc gggaattcga actggtttac gtcacgcgtc
      481 atcaggtggc ggcgcaggcg ctcgcagcga tccggaagta tgcgccccag gcgaaggtcg
      541 tgctcaatct cgccgacctg cacttcctgc gcgagcttcg ggccggcgtg gcggcacagg
      601 atgcgtcccg gatcgacgct gcggtccgca ttcgggactc cgagctcgcc gtgctccgca
      661 cggtcgacct cgcgctgtcc tacagcgccg tcgaagaggc ggtcatcctc agccacaatc
      721 tgaacagcac gaagaccggc cgcctcccct ggatcgccga gccgattgtg gagctgccgg
      781 gctttgcgga gcgcaggaat atcgggttcc tcggcggctt ccgtcacacg cccaatgccg
      841 aagccgtgga tttcttcctt gatcgcgtca tgccggggct gatccgcgag ctgccggacg
      901 ttcagttcga gatttatggc tccgccattt cacgcgagaa ccgcaaacgc tggacgcgcg
      961 aaaatgcagt cgtgaaaggg cgggttgacc gcgtcgagga aatgttgggg catattcgcg
     1021 tgtttgttgc tccgctcgcc tctggcgcgg gcgtcaaggg caaggtgttc gacgcgctcg
     1081 cacacggcgt tcccgccgtg ctgtcgccgt tggcagccga gggcatcggc gttcgcgacg
     1141 gtacggaagt cctgattgcg tgggcgcccg aagagtgggt gaagcagatc gtgcgcctct
     1201 attcggacga gaagctctgg agacaccttt ccgccgccgg ccggcggctg atcgagaccg
     1261 actattcctt tgcgcgtggc gtcgagacga tgcgcgtggc gctcgacaag gcgggcttct
     1321 actgcgagcc ggaccccggc tatctggtgc tcaaccgctg cttgccgtaa agggcggagc
     1381 agcgggagaa tcgccgggca cgatccgaga tgacgatcgt caacaccacc tacaaattca
     1441 tctacgtgca tattcccaag acggcgggaa ctgccgtcaa gaaatatctg ggccgcttta
     1501 cgcgcttctg cgatgtcgaa gtcggagggg cgcgggacgc cgagggggtt gcggaatatt
     1561 accgcaagcg cttcaggctg gccaagcatt cgttcgcgcg cgaaataaag gaggcggttg
     1621 gcgtcgaagc cttccagcat t
//
LOCUS       DASZMC010000539         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2969277,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000539 DASZMC010000000
VERSION     DASZMC010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2969277"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VG274_11000"
     CDS             <1..609
                     /locus_tag="VG274_11000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWE07228.1"
                     /translation="SAQLASDRLARVDDPVVKRLAPRIVASLDHAVTLATRTLRFGRA
                     DEHPPRRERFALAPLVDEVGEAAIPGDAGDFAVRFDNRVDPALEANADHEQLYRMLLN
                     LIRNAAEAVKPRDGGRIEVSAEQENANLAIRVLDNGTGISDLVRPKLFQPFAASTRSA
                     GTGLGLAIARDLARAHGGDVVLESTGESGTCFRIDIPGHEKS"
     gene            660..1433
                     /gene="tam"
                     /locus_tag="VG274_11005"
     CDS             660..1433
                     /gene="tam"
                     /locus_tag="VG274_11005"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: protein motif:HMM:NF002463.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HWE07229.1"
                     /translation="MSWDPKTYLAFGDERTRAAADLLARIGTESPNLVADLGCGPGNS
                     TALLARRWPRARIDGIDNSRAMIEEARATPIPARWIEADVASWAPDGPCDVIYANATF
                     QWVANHEALLPRLMSHLVPHGTLAFQVPRNFHEPSHAIAQEIAGEPRWRDKLARVREW
                     WNVLEPEAYYTLLEPVAARIDIWETRYVQALDGDDAVYRWVLGTGLRPYRDALDGAER
                     EAFLAEYRRRAALAYPRRANGVTLLPFQRLFCIAEGRKL"
     gene            complement(1631..>2381)
                     /locus_tag="VG274_11010"
     CDS             complement(1631..>2381)
                     /locus_tag="VG274_11010"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWE07230.1"
                     /translation="RLAFADRAMWLADPDVVHVPVAGLLDKSYLASRGRLIDPQHDMG
                     TASAGEPPTKHAEFAPQRTPQLPGTSHLTVVDGRGEAVSMTTTVESTFGAEMMAKGFF
                     LNNQLTDFSLEPVRDGKPVANAPAPGKRPMSAMAPTIVFNSDGSLRIALGSPGGPAII
                     PFVAETLVAMLDGGLDPQSAAALPHHVNPNGPTLLEAGTPIADYGSALTAMGHRVVNM
                     ELESGLNIVERVKNGYIGGSDPRRDGVAKGE"
BASE COUNT          410 a          790 c          748 g          433 t
ORIGIN      
        1 agcgcccagc tggcctccga ccgtctcgcg cgcgtcgacg atcccgtcgt caagcggctc
       61 gcgccgcgca tcgtcgcctc gctcgatcat gcggtgacgc tggcgacgcg cacgctgcgt
      121 tttggccgcg ccgacgagca cccgccgaga cgcgagcgct ttgcgctggc gccgcttgtc
      181 gacgaggtgg gcgaggcggc gatccccggc gacgcgggcg atttcgccgt tcggttcgac
      241 aaccgcgtcg atccggctct cgaagcgaac gccgatcacg agcagcttta tcgcatgttg
      301 ctcaatctga ttcgcaatgc tgccgaagcc gtgaaacccc gggacggcgg acggatcgag
      361 gtgagcgcag agcaggagaa cgccaatctt gcgatccgcg ttctcgacaa cggcacgggc
      421 atttcggatc tcgtgcggcc aaaattgttc cagcctttcg ccgcgtcgac gcgctctgcc
      481 ggcaccggtc tcgggctcgc catcgcccgc gaccttgcgc gcgcccatgg cggcgacgtg
      541 gttctcgaat cgaccggtga atcgggcacc tgcttccgca tcgatattcc cggtcacgag
      601 aaaagctgag tgaaccgttc ttccaaatta aatcacaaac taaacgaaac acgctccaaa
      661 tgagctggga tcccaaaacc tatctcgcct ttggcgatga gcgtacgcgc gcggctgcgg
      721 atctgctggc gcgtatcgga acagaatctc cgaaccttgt cgcggatctc ggctgcggtc
      781 ccggcaattc gactgctctt ctcgctcggc gctggccacg cgcgaggatc gacggcatcg
      841 acaattctcg cgccatgatc gaggaagcgc gcgccacgcc gatcccggcg cgctggatcg
      901 aagccgacgt cgcgagctgg gcgcccgatg ggccctgcga cgtgatctat gccaacgcca
      961 cgttccaatg ggtcgccaat cacgaggcgc tgctgccgcg gctgatgtcg catctggttc
     1021 cgcacggaac actcgccttc caggtgccgc gcaatttcca cgaacccagt catgcgatcg
     1081 cgcaggaaat cgcgggggag ccgcgctgga gagacaaact cgcccgcgtg cgcgaatggt
     1141 ggaacgtgct cgaacccgag gcgtattaca cgcttctcga acccgtcgcc gcgcgcatcg
     1201 acatctggga aacgcgctat gtgcaggcgc tcgacggcga cgacgccgtc tatcgctggg
     1261 tgctcggcac gggtctgcgt ccctaccgcg atgcgctcga cggcgcggag cgcgaagcgt
     1321 ttcttgccga ataccggcgg cgcgcggcgc tggcctatcc gcgccgggcg aacggcgtca
     1381 ccctcttgcc cttccagcgc ctcttctgca tcgccgaagg aagaaaactt taacgcggag
     1441 gacgcagagg atgcgcagtg gacgcagaga agaggaagat tcacaatgag acagtgggga
     1501 cagtgaaaaa cttgttgcgc gccttcggcg cgcgatcctt cttcctcact gctctcactg
     1561 tctcattgtg aattcttttg ccggcgtcct ccgcgcatcc tccgcgtcct ctgcgttaaa
     1621 tcttcctggt tcactcgccc ttggccacgc cgtcgcggcg ggggtcggag ccgccgatgt
     1681 agccgttctt gacgcgctcg acgatgttga gtccgctttc gagttccatg ttcaccacgc
     1741 ggtgtcccat cgcggtcaac gccgagccgt aatcggcgat cggcgtgccc gcctcgagga
     1801 gcgtgggccc gttcggattg acgtgatggg gcagtgccgc ggcgctttgc ggatcaaggc
     1861 cgccatcgag catcgcgacc agcgtctctg ccacgaaggg aatgatcgcc ggcccaccgg
     1921 gagagcccag agcaatccgc aagcttccgt cggaattgaa cacgatggtg ggcgccatcg
     1981 cactcatcgg tcgcttgccc ggcgccggcg cattggccac aggttttccg tcgcgcacgg
     2041 gctcaaggga aaaatccgtc agctgattgt tgaggaaaaa acccttcgcc atcatctccg
     2101 cgccgaacgt ggattcgacc gtcgtcgtca tcgaaacggc ttcgccgcgt ccgtccacga
     2161 ccgtgagatg actggtgccg ggcagttgcg gcgtgcgctg gggagcaaac tccgcgtgtt
     2221 tcgttggcgg ctcgcccgcg cttgccgtcc ccatgtcgtg ctgcggatcg atcagcctgc
     2281 cgcgcgatgc caggtaggat ttgtcgagca gccccgcgac cggcacatgc accacgtcgg
     2341 gatcggcaag ccacatcgca cggtcggcga aggcgagccg g
//
LOCUS       DASZMC010000540         9345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2697225,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000540 DASZMC010000000
VERSION     DASZMC010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697225"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /gene="glyS"
                     /locus_tag="VG274_11015"
     CDS             <1..1879
                     /gene="glyS"
                     /locus_tag="VG274_11015"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWE07231.1"
                     /translation="YVALLHRAGRTTAEVLAELLPDAISRLQWPKSMRWGAGAARWIR
                     PLHRIVCIFDGEIVPFTFAGVKSGNATIGHRFMSAGEIEVRGLEDYEEKLWVANVVLD
                     PCRRGEIILHETRQKAFALGTELIEDNALLDEVVGLVEWPVVLTGKIDDDFMNLPPEI
                     LQTSMRVHQKFFALRDIATAKLSDRFVTVANIIADDGGKEIIAGNERVLRSRLADARF
                     FWDQDRKRSLASRLSELRKIVFHARAGSMYDRTLRIALVALLLARTLKFDVEKSCRAA
                     MLAKVDLVTGIVGEFPELQGVMGSRYVLNDGEDSEVADAIRNQYRPVGPADRVPRHAV
                     AIVLALAEKIDSLVALWRAGEKPTGSKDPFALRRAGLGIIRIVLDNGLRLPLSEPMGL
                     VAAALEASDGAIWRSAAGHAVPDHHVLARLAADLFERAVSPAYGNGAPHGTSRPDQAV
                     VPGVLEFLTDRLKVALRDEGVRHDLIDAVFASGGEDDVVRLVSRVRALEAFLETGDGI
                     NLLAAHRRAANILRIEEKRDGRSYEGEPDSGLLEASEEKNLFVALSSARDQIMVEIRE
                     ERFGEAMTCMARLRAPVDAFFDKVTVNADNASLRQNRLLLLAGMRNVLHTVADFSRIE
                     G"
     gene            1884..4553
                     /gene="ppdK"
                     /locus_tag="VG274_11020"
     CDS             1884..4553
                     /gene="ppdK"
                     /locus_tag="VG274_11020"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HWE07232.1"
                     /translation="MPKWLYAFGAGAAEGRADMKALLGGKGANLAEMSRLGLSVPPGF
                     TITTEVCTYFYAHGQTYPPELRPAADAALAHLGEIAHGKLGDSERPLLVSVRSGARAS
                     MPGMMDTVLNLGLNPATVEGLAKLTGDVRFAYDSYRRFIHMYASVVLGLPHENFEAIL
                     ELEKETKGVEYDTDLDADDLRRIADLYRAEVQKQRGSPFPDDVIEQLWGAISAVLMSW
                     NSPRAKTYRRLHRIPDDWGTAVSVQAMVFGNRGGTSATGVAFTRDPSTGEKVLYGEFL
                     ANAQGEDGVAGIRTPQQISKAVREQSGGRKLSMEELMPASFAELKHVAETLEAHYRDM
                     QDVEFTVQEGKLFMLQTRSGKRSVEASLKIAVDMEREGLIDRRQALLRVDPSALDQLL
                     HPTLDTTVATSSIAKGLAASPGAATGEIAFTAEEAEKLAADGRDIILVRMETSPEDIH
                     GMHAAKGILTARGGMTSHAAVVARGMGRPCVSGAGTLKIDALAGVLNIGDRRLGRGDV
                     ITIDGGTGQVFEGRVPMRQPELTGDFGTLMEWADSIRLLRVRANADTPSDAAIARRFG
                     AEGIGLCRTEHMFFDAERIVAVRGMILADTPEEREAALARLLPMQRADFEAIFRVMRG
                     LPVTIRLLDPPLHEFLPQRDEEIAEVARASGGDPARIRQRVAALHETNPMLGHRGCRL
                     GITFPEIYEMQTRAIFEAALNVEREGGDAVDLEIMIPLVGFRSELEMLKTYIANVAAA
                     VRSERGALPDYMIGTMIELPRAALRAGEIAEIAEFFSFGTNDLTQTALGLSRDDAGAF
                     LNEYLKKGLIARDPFVSLDTDGVGELISIASQRGRTSRPGLKLGICGEHGGDPDSIRF
                     CHSCGLDYVSCSPFRVPVARLAAAQAALGTKPS"
     gene            4806..5513
                     /locus_tag="VG274_11025"
     CDS             4806..5513
                     /locus_tag="VG274_11025"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HWE07233.1"
                     /translation="MAKRIGAAARRADLMIAGFLALTFAAAAAAAGAAAIGPSTDKNV
                     QSASFRQIEVAPLPLRLAQADPATTFVSPPPMSWSTAPISFAPDLADPALERMLAEQR
                     CLAEAMYYEARGEGVDGEKAIAEVVFHRMKSARYPHSVCGVVYQGAALKHACQFSFTC
                     SGELQQRKTPGAWARAKRLAAKILGGLVQLGDITEDAISFHAVDVEPGWGDHLVKTIQ
                     IGNHVFYRSALPATQGS"
     gene            complement(5522..6385)
                     /gene="nadC"
                     /locus_tag="VG274_11030"
     CDS             complement(5522..6385)
                     /gene="nadC"
                     /locus_tag="VG274_11030"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWE07234.1"
                     /translation="MNNLDLAFARPPHPLIVEPIVRRALEEDFGRGGDLTTDLIVSSG
                     QHASAKIVARQSGVLAGIIAAECAFRLVDISLRLEPAAMDGQAVAASDLIAHISGSAR
                     GILSAERVALNFIGHLSGVATATHMLVDAVVGTKARIVCTRKTIPGMRALEKYAVRCG
                     GGFNHRFGLDDAVLIKDNHIAAAGGIPSAIERVRQGLGHMVRIEIEVDTLSQLDQALA
                     LGVDTVLLDNMTIEEMKQAVALTRGRAILEASGGVTAASVHAIAETGIDYISVGAITH
                     SARSLDVALDF"
     gene            complement(6382..7965)
                     /locus_tag="VG274_11035"
     CDS             complement(6382..7965)
                     /locus_tag="VG274_11035"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HWE07235.1"
                     /translation="MARSADNVFECEGALILGAGVAGLFTALKLAPFPALVLAAARPG
                     STGSSIWAQGGIAAALGPDDSWASHAADTLAAAAGTGESSVAEFVAREAADRIDDLLR
                     YGAALDRTPDGTLALAREAAHTHARIVHVSGDRAGAEVTRALAQAAKSTPSISVFEGF
                     HALELATVDGRAIGLFARHGARSRLTLFRAQAIIFATGGIGALYAVTTNPPESRGEGL
                     GMAARAGAAIADAEFVQFHPTAIDIGRDPAPLATEALRGEGAILVDEHGRRFMQSVHA
                     AGELAPRDVVARAVHREIAQGRRVFLDCRAAIGSRFALRFPTVYAACIGGGIDPAVQP
                     IPLAPAAHYHMGGIASDHLGRSTLPGLWCVGECASTGLHGSNRLASNSLLEALVFGTR
                     IAEDVSDCGAPAAPSRRTPAPDRFAAAPPPRMLREAMTRNVGLERDEAGLKRALATIA
                     SVERASGSEPSLLNMTAAAKLVATASLLRRESIGAHFRRDFPVAGASRKRSLLTLAAA
                     DSIALEIRPPEESRRSNLQ"
     gene            complement(7973..9004)
                     /gene="nadA"
                     /locus_tag="VG274_11040"
     CDS             complement(7973..9004)
                     /gene="nadA"
                     /locus_tag="VG274_11040"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWE07236.1"
                     /translation="MGMVQMASAAGAEREIYAKVSHVIPEFEWGVHAPYVSAINEIKR
                     RKNAVILAHNYMTPEIFHCVADFVGDSLQLAREAAKNQARIIVQAGVHFMAETAKILS
                     PDKTVLIPDPRAGCSLAASITPADVRLLKERFPGVPVVTYVNTSAAVKAESDVCCTSS
                     NAAAVVEDIARERGVDTVIMIPDEYLAQNVSRQTTVKILSWKGRCEVHERFSAGDIRT
                     YRDANPGVFVLAHPECPPEVIAEADFAGSTSAMIGYVGRHRPARVVMVTECSMSDNVA
                     VEFPDVDFVRPCNLCPHMKRITLPGILQALETMTVEVTVDPAIAARARASVERMLAVG
                     VAKRSLLAA"
     gene            complement(9134..>9345)
                     /locus_tag="VG274_11045"
     CDS             complement(9134..>9345)
                     /locus_tag="VG274_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWE07237.1"
                     /translation="ANLERAGESYDLEPGDVFGEEALEVGTVYERTVTARSEMRLLVL
                     SGDDLRRLVRKFALLRNRLLNEAAW"
BASE COUNT         1814 a         2783 c         2980 g         1768 t
ORIGIN      
        1 ctatgtcgcg ctcttgcacc gcgcgggccg cacaacggcc gaggtcctgg cggagctgct
       61 tccggatgcg atttcgcgtt tgcagtggcc gaaatcgatg cgctggggcg ccggcgctgc
      121 gcgctggatc cggccactcc accggatcgt gtgcattttt gacggtgaga tcgtgccgtt
      181 cacatttgcg ggcgtgaaaa gcggcaatgc gacgatcggc catcgcttca tgtcggcggg
      241 cgaaatcgaa gtgcgcggcc tggaagatta tgaggagaaa ttgtgggttg caaatgtcgt
      301 tctggatccg tgtcggcgcg gggaaatcat ccttcacgag acccgacaaa aggcattcgc
      361 gcttggaacg gagctcatcg aagacaatgc gctcctggat gaggtcgttg gactcgtcga
      421 atggccggtc gtgctgactg gcaagatcga cgatgacttc atgaacctgc caccggagat
      481 attgcagact tcgatgcgtg tgcaccaaaa attctttgcc ctgcgggaca ttgctacggc
      541 aaagctttca gaccggttcg tgacggtcgc gaacatcatt gccgatgatg gaggcaagga
      601 gattatagct ggcaatgaac gcgtgcttcg gtcgagactg gccgacgcca gatttttctg
      661 ggatcaggac cgaaagcgtt cgctggcttc ccggttgtct gagctccgaa aaatcgtgtt
      721 tcacgcgagg gcgggaagca tgtacgaccg aacgcttcgg atcgcgctcg tcgccctgct
      781 tctcgctcga accctgaaat tcgatgtgga aaaatcgtgc cgcgccgcga tgcttgcgaa
      841 ggtcgatctg gtaaccggca ttgtgggtga atttccggaa cttcaaggcg tgatgggttc
      901 cagatacgta ctgaatgacg gtgaagattc cgaagtcgca gacgctatcc gaaatcaata
      961 caggcccgtc gggcctgccg atcgtgttcc ccgccatgcc gttgcaatcg tgctcgctct
     1021 ggcggaaaag attgacagtc tcgtcgcgct ctggcgtgcc ggcgagaaac cgaccggctc
     1081 caaggacccg tttgcgttgc ggcgagccgg tcttggaatc attcgcatcg ttctcgacaa
     1141 cggcttgcgc ctcccgcttt cggagccgat gggattggtc gccgcggcat tggaggcgag
     1201 cgacggcgcg atctggcgat cggctgccgg gcacgcggtg cccgatcatc acgtgctcgc
     1261 aaggcttgcg gcggatttgt tcgagcgcgc ggtctcgccg gcctacggca atggcgcgcc
     1321 gcacgggaca tcgcggccgg atcaagcggt agtgcccgga gttctggaat ttctgacgga
     1381 tcggctcaaa gttgcattgc gcgacgaggg cgtgaggcat gatctgatag acgcggtatt
     1441 tgcgagcggt ggagaggacg atgtcgtccg cctcgtttcg cgtgttcgcg cgctggaggc
     1501 ttttctggaa acaggcgatg gtatcaacct gctggcagcg catcggcgcg ccgcgaacat
     1561 cctcaggatc gaagaaaagc gggacggccg gagttatgaa ggcgagcctg attcgggcct
     1621 gcttgaggcg tcagaggaaa aaaatctgtt cgtggccctc tcatcggcac gcgatcagat
     1681 catggtcgag attcgagagg aacggttcgg tgaagcaatg acctgcatgg cgcggctccg
     1741 cgcacctgtc gatgctttct tcgacaaggt gacggtcaat gccgacaacg cgtcgctgcg
     1801 ccaaaatcgc ttgctgcttc tggccggcat gaggaatgtt ttgcacaccg tagcggattt
     1861 ttcccggatc gaaggttaag cagatgccca aatggctgta cgcgttcgga gccggcgcgg
     1921 cggaggggcg cgctgatatg aaggctctgc ttggcggcaa gggtgcaaat ctggccgaga
     1981 tgagcaggct cggcctgtcc gtgccccccg gcttcaccat cacgacggag gtctgcacct
     2041 acttctatgc acacggacaa acctatcctc ccgagttgag acctgcggcg gacgcggccc
     2101 tcgcgcattt gggtgagatt gcccacggga aattgggcga cagcgagcgt ccgcttctgg
     2161 tttcggtgcg ttcgggcgcc cgggcgtcga tgccgggcat gatggacacg gtgctcaatc
     2221 tgggtctcaa tccggcaacg gtcgagggtc tcgcgaaact caccggcgat gtccgctttg
     2281 cctatgattc ctaccggcgc ttcattcaca tgtacgcgag cgtcgttctc gggctgcccc
     2341 acgaaaattt cgaggcgatt ctcgagcttg agaaggaaac caaaggcgta gagtacgata
     2401 cggacctcga tgcggacgat ctgaggcgca tcgcagatct ctatcgggcc gaagtgcaga
     2461 agcagcgcgg ctcgccattt cccgacgatg tgatcgaaca gctttggggc gcgataagcg
     2521 ccgtccttat gtcctggaac agtccgcggg cgaagacata ccgcaggctt cacaggattc
     2581 cggatgattg gggcaccgct gtaagcgtgc aggcaatggt gttcggcaat cgcggtggca
     2641 ccagcgcgac aggtgtggcc tttacgcggg atccttcgac cggggagaaa gtgctgtatg
     2701 gcgagttcct cgctaatgcg cagggcgagg acggtgtggc ggggatccga acgccgcaac
     2761 agatttcaaa agcagtgcgc gaacaatcgg gtggccgcaa actgtccatg gaagagctca
     2821 tgccggcctc ttttgccgag ttaaagcacg tcgccgaaac gctcgaagcc cattaccgcg
     2881 acatgcagga tgtggaattc accgtgcagg agggaaagct gttcatgctg cagacgcgct
     2941 ccggcaagcg gtcggtagaa gcatctctca agattgccgt cgatatggaa cgcgaggggc
     3001 tcatcgacag gcgccaggcg ctgcttcgcg tcgatccgtc cgcactggat cagctccttc
     3061 atccgacact cgacacgacg gttgccacat ccagtatcgc gaaaggtctg gcggcgtctc
     3121 cgggcgccgc caccggcgaa atcgcgttta ctgccgagga ggcggaaaaa ctggcggcgg
     3181 acggacggga catcattctc gtacgcatgg aaacgagccc ggaagacatc cacggcatgc
     3241 acgccgcaaa aggaattctc acggcacgag gcgggatgac gagccacgcc gcggtcgtcg
     3301 cgcgcggtat gggccgacca tgcgtgtcgg gcgccggtac gctcaagatc gatgcgctgg
     3361 cgggcgtatt gaacattggc gatcgccggc tcggccgtgg cgacgtcatc acgatcgacg
     3421 gaggaacggg gcaagtgttc gagggacgcg ttcccatgcg gcagcccgaa ctgacgggcg
     3481 acttcggcac gttgatggag tgggcggatt ccattcgcct gctgcgggtc cgcgcaaatg
     3541 cggacacgcc atccgacgcg gcgatcgcac ggcgcttcgg cgccgaaggc atcggactct
     3601 gccgcaccga acacatgttc tttgatgccg agcggatcgt cgcggttcgc ggcatgatcc
     3661 tcgccgacac gcccgaagag cgtgaagccg cacttgccag gctgctgccg atgcagcgtg
     3721 ccgatttcga ggcgatattc cgcgtcatgc ggggccttcc ggtgacgatc cgccttctcg
     3781 atccgccact gcacgaattt ctgccgcaac gggacgagga aattgccgag gtggcccgcg
     3841 cgtccggcgg cgaccctgcg cggattcggc agcgcgtggc cgcgctgcac gaaaccaatc
     3901 ccatgctcgg ccacaggggc tgcaggctcg gcattacctt tcccgagatc tacgagatgc
     3961 aaacgcgcgc cattttcgaa gcagccctca acgtcgaacg cgaaggaggc gatgcagtcg
     4021 atctcgagat catgattcct ctcgttggat tccgcagcga gctcgaaatg ctgaagacgt
     4081 atattgcaaa tgtcgctgcg gcggtgagaa gcgagcgcgg tgcgctcccg gattacatga
     4141 tcggcacaat gatagaactg ccgcgcgctg ctctcagggc aggtgagatt gcggagatag
     4201 ccgagttctt ctcgttcggc acaaacgacc tcacccagac ggcgctggga ctgagccgcg
     4261 acgacgcagg agcgtttctg aacgagtacc tgaaaaaggg tctgatcgct cgtgatccct
     4321 ttgtaagcct cgatacggac ggcgtcggcg aattgatttc aatagcgtcg cagcgcggcc
     4381 gtacgagccg ccccggactc aagctcggta tttgcggcga gcatggcggg gatccggact
     4441 cgatccgttt ttgccactca tgcgggcttg attacgtctc gtgctcgccg ttccgtgttc
     4501 ctgtcgcacg acttgccgcg gcccaggcgg cgctcgggac caaacccagt taatccgtac
     4561 acggttaatt aacccgcaat tctccagtca ccttaaagct tttcatgctg aacatgacat
     4621 cgagtcacgt gatgcgtgac tcgatgtcag aatatcagtg acatcttgtc acgttctgga
     4681 acgtaattca gttgaccgtg cggctcttcg ctggcagaag gcggctactc gagagcgggg
     4741 aaggggaccc cttctgaaca agacatcgaa tttcttcgaa gccacatccg agagcgaatt
     4801 cccgaatggc gaaaagaatt ggtgctgcgg cgcgacgcgc cgatctcatg atagcaggat
     4861 ttctggcgtt gacatttgcg gccgctgcgg ccgctgcggg cgccgcggcg attggtccgt
     4921 ctactgacaa aaacgtccag agcgcttcct tccggcagat cgaggtagcg cccttgccct
     4981 tgcggctggc gcaggcagat ccggcaacca cgtttgtcag cccgccgccc atgtcgtggt
     5041 cgaccgcgcc gatttcgttt gcgccggatc tggccgatcc tgcactcgaa aggatgctgg
     5101 ccgagcaacg ctgtcttgcg gaagcgatgt actacgaggc gcggggcgaa ggcgtggatg
     5161 gcgagaaggc gatagccgag gtcgttttcc atcgtatgaa atcggcacgc tatccgcatt
     5221 cggtctgcgg tgttgtctat cagggcgcag ccttgaaaca tgcctgccag ttttcgttca
     5281 cctgcagtgg cgaactgcag cagcgcaaga cgccgggagc gtgggcgagg gcgaagcggc
     5341 tggcggcgaa aattctaggt gggttggttc agctcggaga catcaccgaa gacgcgatct
     5401 cgttccacgc tgtggacgtg gagccagggt ggggcgatca cctggtcaaa accatccaga
     5461 tcggcaatca cgtgttctac cggtcggcgc tgccagcgac gcaaggcagc tgaacgacgg
     5521 tctaaaaatc cagggcaacg tccaaagagc gcgcgctatg ggtgattgcg ccgacggaaa
     5581 tgtagtcgat tcccgtttcg gcaattgcat gcaccgaggc agcggtaacg cctcccgacg
     5641 cctccagaat ggcccgtcca cgcgtcaggg cgaccgcctg tttcatctcc tcgatcgtca
     5701 tattgtcgag aagaaccgtg tcgacgccca gcgcaagcgc ctgatccagc tgggagagag
     5761 tgtccacctc gatttctatt ctcaccatat ggcccagccc ctggcgcacg cgctcgattg
     5821 ccgacgggat tccgcccgcg gctgcaatgt ggttgtcttt gatcaggacg gcgtcgtcga
     5881 gcccgaagcg gtgattgaac cccccgccgc accggaccgc atatttctcg agcgcccgca
     5941 tgccggggat tgttttgcgc gtacagacga tccgcgcttt cgtgccgacg acagcgtcga
     6001 caagcatgtg cgtggcggtg gctacgcccg acagatggcc aatgaaattc agcgcgaccc
     6061 tttccgcact caagatgccg cgcgcgctgc ccgagatgtg cgcgatgaga tcggaggcgg
     6121 cgaccgcttg cccgtccatt gcagcaggct cgagccggag agagatatcg accagccgga
     6181 acgcgcattc cgcggcgatg atgcccgcca aaacgcccga ttgacgcgcg acgattttgg
     6241 ccgaagcgtg ctgcccggac gaaacgatga ggtccgttgt caggtcgccc ccgcggccaa
     6301 aatcctcttc gagcgcccgt ctgacaatgg gttctacgat cagtgggtgc ggtggccgcg
     6361 caaaggcaag gtcaaggtta ttcattggag attcgagcgg cgggattctt caggcggacg
     6421 aatctcgagc gctatgctgt ccgctgcagc gagcgtgagc aggcttcgct tgcgcgatgc
     6481 gccggcgacc ggaaagtcgc ggcggaagtg tgcgccgatg ctttcccggc gcaaaagcga
     6541 cgcggtcgca acgagcttgg ccgccgcggt catgttgaga agtgagggtt cgcttcccga
     6601 tgcacgttcc acgctcgcaa tcgtggcaag cgcgcgcttg agcccggctt catcgcgttc
     6661 gagtccaacg ttgcgcgtca tggcttcccg caacatgcgt ggcggcggcg cggcagcgaa
     6721 ccggtccggc gccggagtcc gccgcgaagg cgcggccggc gcgccacaat cgctaacgtc
     6781 ttccgcgatg cgggtgccga agacaagcgc ttccagcagg gagttcgaag caagacgatt
     6841 tgatccgtgc agacctgtcg acgcacattc gccgacgcac cagagacccg gcagcgtcga
     6901 gcggccaaga tggtcgctgg caattcctcc catgtgatag tgcgcggcag gcgcaagcgg
     6961 aatcggttgc acggccggat cgataccgcc gccgatgcag gccgcatata cggtaggaaa
     7021 ccgcaatgca aagcggcttc cgatcgccgc gcggcagtcc aggaagacgc ggcgtccctg
     7081 tgcaatttcg cgatggacgg cacgcgcaac gacgtcgcgc ggtgccagct ctcccgcggc
     7141 gtgaaccgac tgcatgaagc gccggccatg ctcatccacc aggattgcgc cttctccgcg
     7201 cagcgcttct gtcgcgagcg gcgcaggatc gcgcccgatg tcgatcgccg tcgggtggaa
     7261 ctgcacaaat tccgcgtcgg cgatcgccgc gccggcgcgc gctgccatgc cgaggccctc
     7321 tccccgcgat tcgggaggat tggttgtaac ggcgtaaagg gcgccgatac caccggtagc
     7381 gaagatgatg gcctgggcgc gaaagagcgt cagtctcgat cttgctccgt ggcgcgcaaa
     7441 caacccgatt gcgcgaccgt cgacggtggc gagttcaagc gcgtgaaatc cctcgaaaac
     7501 agagatcgac ggcgtggact tggccgcctg ggcgagcgcc cgtgtaacct cggcgccggc
     7561 gcggtctccg gaaacatgga cgatcctcgc atgggtgtgg gcagcctcgc gcgcgagggc
     7621 cagcgtgccg tcgggcgtgc ggtcaagggc cgcgccatag cgcaagagat cgtcaatccg
     7681 gtcggctgct tcacgggcaa cgaattcggc aacgctgctt tcgccggtgc ccgctgccgc
     7741 agcaagcgtg tcggccgcat gtgacgccca gctgtcgtcc ggtcccaatg ccgccgcgat
     7801 cccgccttgc gcccaaatgc tcgatccggt cgagcccggg cgggccgccg caagcacgag
     7861 ggctggaaag ggtgcgagct taagcgccgt gaaaaggcct gcgacgccgg cgccgagaat
     7921 caacgcacct tcgcattcga agacattgtc cgcggagcgc gccatctcgc gactaggccg
     7981 ccagcagact ccgctttgcg acgccaaccg caagcattcg ttcgacggat gcccgcgctc
     8041 gcgcagcaat cgcggggtcg accgtcacct ccaccgtcat ggtctcgagc gcctgcagga
     8101 tgcccggcag cgtaatccgc ttcatatggg ggcaaagatt gcagggacga acgaagtcca
     8161 cgtccggaaa ttcaacggcg acattgtcgc tcatggagca ttcggtgacc atcacgacac
     8221 gagccggccg atgcctgccc acatagccga tcatggccga agtcgatccg gcaaaatccg
     8281 cttcggcgat tacttcgggc gggcattccg gatgggccag gacgaaaacg ccggggttgg
     8341 cgtcacgata ggtccggatg tcgcccgccg aaaaacgttc atgcacttcg catcggccct
     8401 tccatgacag gattttcacc gtcgtctggc gcgaaacatt ctgggcgaga tactcgtccg
     8461 ggatcatgat tacggtatcc acgccgcgct cgcgggcgat atcctcgacc accgccgcgg
     8521 cattcgagga cgtgcagcat acgtcgctct ccgcctttac cgccgccgac gtgttcacat
     8581 aggtgacgac gggaacgccg gggaatcttt ccttgaggag gcgcacatcc gccggggtga
     8641 tggacgccgc gagtgagcat ccggcgcggg gatccggaat gagaactgtt ttatcgggcg
     8701 agagaatctt tgcagtctcc gccatgaagt gaacacccgc ttgcacgata attctggctt
     8761 ggttcttcgc ggcctcgcgc gccagctgaa gcgagtcgcc cacgaagtcg gcgacgcaat
     8821 ggaagatttc cggtgtcata tagttgtgcg caaggatcac cgcgttcttg cgccgcttga
     8881 tctcgttgat cgcgctcaca tagggagcgt gaactcccca ctcgaactcc ggaatgacat
     8941 gggaaacctt cgcgtaaatc tcccgttcgg cgccggcggc ggaagccatt tgaaccatac
     9001 ccatccgtct tctccctgcg ttccggctca ataaatgctc tatttgagta tatataaggt
     9061 ccaagagtgc ccgatcaagg cccgcaatgc ggtcatttat gctacacaat agcataaatg
     9121 ggtcaagggg cagtcaccag gcggcttcgt tgagcaatcg gttgcggagg agggcgaatt
     9181 tgcgcaccag gcgccgcaga tcgtcgccgg aaagaaccag aagacgcatc tctgagcgcg
     9241 ccgtgaccgt tcgttcgtat accgttccga cttcaagcgc ttcttccccg aacacgtcac
     9301 cgggctcgag atcataggac tctccggcgc gctcgagatt cgcgc
//
LOCUS       DASZMC010000541         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1621706,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000541 DASZMC010000000
VERSION     DASZMC010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621706"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..782)
                     /gene="lptE"
                     /locus_tag="VG274_11050"
     CDS             complement(258..782)
                     /gene="lptE"
                     /locus_tag="VG274_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWE07238.1"
                     /translation="MSARIAAAVVLMSVALSASCGYSTGGRGELLPTTIHTIAVPAFV
                     NATTRYKITDGMAEAITREFITRTRYKIVSNPNEADAVLHGTVINYTSATTVLDPTTG
                     RASAVDIHVYLRLDLTERTTSKVLFTRPFMDVRERYQISTDAAAFFEESDEAIKRASE
                     QTARQVVSAILETF"
     gene            complement(779..1384)
                     /locus_tag="VG274_11055"
     CDS             complement(779..1384)
                     /locus_tag="VG274_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HWE07239.1"
                     /translation="MLLAALSRRTTIRRSLVHVFEPASERGQKGLDELQQQTIATLNF
                     RKLPTDVFDAQLSFNMLARYGEEAMEPLEGIEQRIEKHVASLLSAHPAVPMPSLRVIQ
                     APVFHGHSFSLWVEFAENPGVKALGEILTEAGIDVRPDDPPSNALIAGQSGLSAGAIT
                     VDSNQPRAAWIWAVADNFRLVAENAVAAAQPFLTDAQEPRQ"
     gene            complement(1827..2723)
                     /gene="pssA"
                     /locus_tag="VG274_11060"
     CDS             complement(1827..2723)
                     /gene="pssA"
                     /locus_tag="VG274_11060"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWE07240.1"
                     /translation="MAKMTWRDRLIDPRSPERRPRRAAYALPTLFTAGNIFLGFYAII
                     ELFKAALQMPTSGAATIPHFRLASIAIGAAVFLDGLDGWIARATNTVSDFGREMDSLA
                     DVITFGIAPAILAFVWGVQFVDVAIDQMTLEYLRRAGYFVTFLFLLCGAMRLARFNVQ
                     KNPIPSNPGRPDKKYFVGMPIPSAAGMVASVVYAAESEPLHNWIISAAWLCLVALLSF
                     LMVSTWRYPSFKGVNLLKPRSPLIVVLLGSSVYLIIYFSQPLLLGMCAFYVGSGIVIR
                     IGGFVRRRLRHQPPSHPEHQVG"
     gene            complement(2737..>3125)
                     /locus_tag="VG274_11065"
     CDS             complement(2737..>3125)
                     /locus_tag="VG274_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HWE07241.1"
                     /translation="FDVHVNRTPIPGRIAKIEYKEGQFLNASVKDASHLNEQNVVTVD
                     GEFNGHKTRVVFSQIAGLIARRIIFYKQTGDVVKPGERVGLIKFGSRVDVFFGPEWEI
                     VVRAGDRVSAGSSVVARFRNASNQGV"
BASE COUNT          621 a          882 c         1042 g          580 t
ORIGIN      
        1 ctcgctgagg tcgtgccgcg caaagccgtt ctcgcgcgct tcgggatcct cgccgagcgc
       61 gacgcggatc agctcgcggc ggcagcgctc gcgctgatag gattcgggtc ccaggaataa
      121 gtagacgggc gccgggccgc ttttttgcag gccggagaga aactggtcgg gcgacattgt
      181 aagttttatg atcgctcaca agaactgtgc gcgcgagaaa tcacgagaca gaagtcggga
      241 gacagaatgg gaatccgtca gaaagtttcg aggatggcgg agacgacctg gcgggcggtt
      301 tgttcgctgg cgcgtttgat ggcttcgtca ctttcttcga agaaggcggc ggcgtctgtg
      361 gagatctgat agcgctcgcg aacgtccatg aagggtcggg tgaacagcac cttgctggtg
      421 gtgcgctctg tcaggtccaa acgaagatag acgtgaatgt ccacggcgct ggcgcgtccg
      481 gtggtgggat ccaatacggt ggtagcggac gtatagttga tgaccgtccc gtgcaggacg
      541 gcgtcggcct cgttgggatt cgagacgatc ttatagcgcg tacgcgtgat gaattcgcgc
      601 gtgatggctt ccgccatacc gtccgtgatc ttatagcgcg tggtggcatt cacaaaagcc
      661 ggcacggcga tggtgtgtat ggtcgtgggc agcagttcgc cgcggcctcc ggtggaatag
      721 ccgcaggatg cggagagcgc gacgctcatg aggacgacgg ctgctgcgat gcgcgcactc
      781 attggcgagg ctcttgcgcg tcggtgagaa acggttgcgc cgcggctacg gcgttctcgg
      841 caaccagccg gaagttatcg gccacggccc aaatccacgc ggcgcgcggc tggttggaat
      901 cgacggtgat ggcgcccgcg cttaagccgc tttgtccggc gatcaacgcg tttgagggag
      961 gatcgtcggg gcgcacgtcg atgccggcct ccgtgagaat ttcgccgagc gctttgacgc
     1021 cggggttttc cgcgaactcc acccacagtg aaaaactgtg accgtgaaag acgggagcct
     1081 ggatgacgcg caacgaaggc atgggcacag ccgggtgggc cgacagcagg gacgcaacgt
     1141 gtttttcaat gcgctgctcg atgccctcga gaggctccat cgcttcttcg ccgtaacgcg
     1201 ccagcatgtt gaagctgagt tgcgcgtcga aaacgtcggt cggcagctta cggaagttca
     1261 gcgtggcgat agtctgctgc tgcaattcgt cgagtccttt ttgtccccgc tcgctggccg
     1321 gttcgaaaac atgcaccagc gaccggcgaa tagtggtgcg gcgagagagc gcggcgagca
     1381 gcatagcaat ggcgatggcc gcgggatgcg ccaccacttg tatggaggac gcgctcacgg
     1441 cgcccccggt ttccgcggtt tcggtggagg gcgcgcgcag gcgtgcttgc ggctgatctt
     1501 ccagggctcc ggtgagatcg atcagcactg gaccaccggg cggattcaag cgcgcggctt
     1561 tgcggctgga ggcgggtgag cccgcgagga atgcgacgcc cgcgcccgcg aggctcgcgg
     1621 cggtgagagg gctcaaagcg atggcctctt cttcgccgct ttttacgtcc agcgcgagca
     1681 ccgatgcatt ttcagatgcc ccggaaatca gctcgatagc gggcgcgggt tccgcttcgc
     1741 gcaagaggtc gcggagttca cgcgcgagta acgagtcgcc gccaacgata gctacacggc
     1801 gccgggggct ctcagctgca cgttccttag ccaacctggt gctccggatg cgaaggtggt
     1861 tggtggcgga gtctgcggcg cacaaaacct cctatgcgaa tcacaatgcc gctaccgacg
     1921 tagaaggcgc acattcccag cagcaagggc tgcgagaaat agatgatgag ataaaccgag
     1981 cttcccagca ggacgacaat gagcggtgaa cgcggcttca gcaggttcac acccttaaaa
     2041 ctgggatagc gccacgtgct gaccatcagg aaagaaagca gcgcgaccag gcacagccag
     2101 gccgccgaga taatccaatt gtgcagcggt tcgctttcag cagcgtaaac gacggaggcc
     2161 accatgccgg ccgccgacgg gatcggcatt ccgacgaaat attttttatc cggacggccg
     2221 ggattcgatg gaatggggtt cttctgaacg ttgaagcgcg ccagacgcat ggcgccgcac
     2281 agcaggaaca ggaaggtgac gaagtagccc gcgcggcgca ggtattccag tgtcatctga
     2341 tcgatggcga cgtcgacgaa ttgcacgccc cagacgaaag caagaatcgc gggcgcgatg
     2401 ccgaaggtga tcacgtcggc gagcgaatcc atctcgcgtc caaaatcgct gaccgtgttg
     2461 gtcgcgcgcg caatccaccc atccagcccg tcgagaaaga cggcggcgcc gatggcgatg
     2521 gaggcgaggc gaaagtgcgg aatggtggcc gcgccggaag tcggcatttg aagggcggcc
     2581 ttgaagagtt caatgatggc gtagaagccc aggaatatat tgcccgcggt gaacagcgtc
     2641 gggagtgcat aggcagcgcg ccgcggacgc ctctcaggcg agcgaggatc gatgagacga
     2701 tcgcgccagg tcatttttgc catgcgttgc cctctctcat accccttgat tcgacgcgtt
     2761 tcggaaacgc gccacaacgc tcgatccggc gctgacgcgg tcgcccgcgc gtaccacgat
     2821 ttcccattcg gggccaaaga acacatcgac acgagagccg aatttgatca gtccgacacg
     2881 ctcgcccggc ttgacgacat ctcccgtctg cttataaaaa atgatgcgcc gcgcgatgag
     2941 tccggcgatc tgcgaaaaaa cgacgcgcgt tttgtggccg ttgaactcac cgtccacggt
     3001 gacgacattc tgctcgttca ggtggctggc gtctttcacg cttgcgttga ggaactggcc
     3061 ctctttgtat tcgatcttcg cgatgcgtcc ggggatgggc gtgcggttca cgtgcacatc
     3121 gaaga
//
LOCUS       DASZMC010000542         4698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5166935,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000542 DASZMC010000000
VERSION     DASZMC010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5166935"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VG274_11070"
     CDS             <1..1225
                     /locus_tag="VG274_11070"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07242.1"
                     /translation="VLRDNYDQTLALSVAVARSARDLDSHARFIRDLERRGRLDRAVE
                     FLPDEDELHRRAQLGLGLTRPELAVLLAYAKLDLDAQIVASDLPDQAFFASELAAYFP
                     ASAVMRLPDELIHHRLRREIIATVLANRIINLGGPVFVHRIEEVSSAPGALIARAFVL
                     AEGALGLTALKSRIDALDGKVRASVQIGAYNEIAELLRRLGLWFLVNVPARAGLGDTI
                     ARYRAGVESLRGSFSTLVSPYEAQDTETRIAELQKAGVPLTVAEDVAVLPLMGGAPEI
                     VLLAEARGLPIDLVAGAYFAVGAAVGLDRLRGLAGRIAGHEHWDRLAVRRIVDDLFAG
                     QRALAADALRDADRSLEGNMRAQGADAVKRWENTHSDGLARTRSFLAELERSGELSIA
                     KLTLANSQIHALAAQ"
     gene            1401..1778
                     /locus_tag="VG274_11075"
     CDS             1401..1778
                     /locus_tag="VG274_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07243.1"
                     /translation="MLRRLVAISICGLLAACATARKPIQPQAPLVALPPAPPPGEPSD
                     LAGLRSAQLQIVFGAPAFVRREGGAEMWRYDGAACKVFFFLYPYAGSLLVRHVETLPR
                     GREMAADQACLDSLRPRAPAPVS"
     gene            complement(1727..2566)
                     /gene="nth"
                     /locus_tag="VG274_11080"
     CDS             complement(1727..2566)
                     /gene="nth"
                     /locus_tag="VG274_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWE07244.1"
                     /translation="MVIRAHGLRMRFCAEMDKEAGTCRAVRIAEERQMRQCRGETFRE
                     AAGLAKPFSKKECETFFERLKQQMPEPKTELVYSNPFTLLIAVVLSAQSTDKGVNKAT
                     QALFRVADAPETMVRLGEEGLIAHIRTIGLFRSKAKNVIALSRILIAEHDGKVPRDRA
                     ALEALPGVGRKTANVILNVAFGEPVIAVDTHIFRVANRTGLAPGKTPLAVERKLASVV
                     PEKYRLHAHHWLILHGRYTCVARRPKCPGCVVRDLCRYTKKTLAAQETGAGARGRSES
                     RQA"
     gene            2549..3034
                     /locus_tag="VG274_11085"
     CDS             2549..3034
                     /locus_tag="VG274_11085"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HWE07245.1"
                     /translation="MSADNHPVLFDQTLRPNPPMNIGALVFVATAVATINLAFGLYFV
                     LRGAWPVTPFMGLDAILLAWAFRASFRTARCYERICLTPTMLRVEHHPARGRPIRSEF
                     NPYWVRVDFGEAPGRGRSLALASHGLFVQIGAFLPPAQKLSVAQGLTAALARSRQTNF
                     D"
     gene            3084..3947
                     /locus_tag="VG274_11090"
     CDS             3084..3947
                     /locus_tag="VG274_11090"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWE07246.1"
                     /translation="MNMHAIDHEPAEDSSHYRRIARAVRFLAENYRDQPTLEEAAGAA
                     GLSPFHFQRLFSRHVGVSPKSFVANLTLGHAKTRLAQGASVLEAALDSGLSGPSRLHD
                     LCLKIEAMTPGEFAREGRGLHIAHGFCDSPFGRTLVMSTVQGVCGLAFADEEEMPEAL
                     TAMKSRWPQAEYFEDAPYIEVLGEKIFAPQPGGHLTLHLIGTRWQIKVWQALLAIPPG
                     TVVSYRTIAQHVRNAHDARAVGGAVGSNPIAWLIPCHRVLASDGTLHGYHWGLARKRS
                     MLAVEAARKAA"
     gene            complement(3969..>4698)
                     /gene="dapB"
                     /locus_tag="VG274_11095"
     CDS             complement(3969..>4698)
                     /gene="dapB"
                     /locus_tag="VG274_11095"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWE07247.1"
                     /translation="AFSLAGALEAPAHPDLGTDAGRMVHLPPADIPVAEDARAALAGA
                     DALIDFTTPSVSVALAALSAQARIAHVIGTTGFSTAEEERIRVVAREAVIVKSGNMSL
                     GIALLAALVKRAARALPDFDVEIVEMHHRMKVDAPSGTALLLGRAAAEGRGVDLGTHV
                     APPRTGRTGARKDGSIGFASLRGGTMAGEHKVILAGPQERVVLEHVAEDREIFARGAL
                     AAAKWARGQKPGLYTMADVLGLAG"
BASE COUNT          798 a         1512 c         1448 g          940 t
ORIGIN      
        1 tgttctgcgc gacaactacg atcagacgct tgccctttcc gtagcagtgg cgcgcagcgc
       61 gcgggatctc gattcgcatg cgcgattcat ccgtgatctg gaacgaagag gccgtctcga
      121 ccgcgccgtt gaatttcttc ccgacgagga cgaattgcac cgccgcgcgc agctggggct
      181 cggcctcaca cgtccggaac tggccgtgct gctggcttac gccaagctcg acctcgacgc
      241 ccagatcgta gcgagcgatc ttcccgatca ggcgttcttt gcctccgaac ttgccgccta
      301 ctttccagcc tcggccgtga tgcgcctgcc cgatgaactc attcatcacc ggttgcggcg
      361 cgagatcatc gcgaccgtgc tcgccaatcg aattatcaac ctgggcggcc cggttttcgt
      421 gcaccgcatc gaggaagttt ccagcgcccc cggcgcactg atcgcgcgcg cctttgtgct
      481 cgcagaaggc gcgctgggtt tgaccgcact caaatcccgt atcgacgcgc tcgatggcaa
      541 agtgcgcgcg tcggtgcaga ttggcgcata caatgaaatt gccgaacttc ttcgccgcct
      601 ggggctgtgg ttcctggtca atgtccccgc gcgcgccggc ctcggcgaca cgattgcgcg
      661 ctatcgcgcg ggcgtggaat ccctgcgggg aagtttttcg actcttgttt cgccttatga
      721 ggcgcaggat accgaaacgc gcattgctga actgcagaag gccggagtcc cccttactgt
      781 agcggaggac gtcgccgtac tgcccctgat gggcggcgct cccgaaatcg tgcttctggc
      841 cgaggcgcgc ggcttgccga tcgatctggt ggcaggtgcg tattttgcag tcggtgcggc
      901 agttgggctc gacaggctgc gagggctcgc aggccggatt gccggccacg aacattggga
      961 ccggcttgcg gtgcggcgga tcgtggatga tctgttcgcc ggacagcggg cactcgccgc
     1021 cgatgcgctc cgtgacgccg accgctctct tgaaggcaac atgcgcgccc agggcgcaga
     1081 cgccgtcaag cgttgggaga acacgcactc cgacggactc gcgcgcacca gaagcttcct
     1141 ggccgaactc gaacgcagcg gcgagctgtc gatcgccaag ctcacccttg cgaattcgca
     1201 gattcacgcg ctcgccgcgc agtgatttga gtggcccggc aatgagtgtt acgtgacgat
     1261 ggctctcgtg gttcgcctcg ccgcggctcg catttgtggc gcggcgggcg aactcatcat
     1321 gagggcccca tcctgagctt gccaaaggat gaggtccgat tcaggtgtca acgtcagtgg
     1381 gtgccaggct caacaaaggc atgcttcgcc gtcttgtcgc gatctcgatc tgcggcttgc
     1441 ttgcagcctg tgcgaccgcg cggaagccaa tccaaccgca ggcgccgctc gtcgcgctac
     1501 cgcctgcgcc gccgcccggc gaaccgtccg atctcgcagg cctgcgatcc gcacaactgc
     1561 agatcgtttt cggcgcgccg gccttcgtgc gcagggaggg aggcgccgag atgtggcgtt
     1621 acgatggggc tgcgtgcaaa gtcttctttt tcctctatcc ttatgccggc tcgctgctcg
     1681 ttcggcatgt ggagacgctg ccgcgcggac gcgaaatggc ggcagatcag gcctgtctcg
     1741 attcgctgcg tccgcgcgca ccggcgccgg tttcctgagc ggccagcgtc tttttcgtat
     1801 agcggcacag atcgcgcacg acgcaacccg ggcattttgg ccttcgcgcg acgcaggtgt
     1861 agcgaccgtg caatatcagc caatggtggg cgtgcaggcg atatttctcc ggcacaactg
     1921 aagcaagctt cctttcgacc gcgagcggcg tcttgcccgg ggcgaggccg gtgcggtttg
     1981 cgacccggaa gatgtgggtg tcgacggcaa taacgggctc accgaaggcg acattcagga
     2041 tgacgttcgc cgtctttcgt ccgacgccgg ggagcgcctc gagcgctgcc cggtcgcgcg
     2101 gcacttttcc atcatgttca gcgatcagaa ttcgcgatag cgcgatgacg ttcttcgcct
     2161 tgctgcgaaa caggccgatc gtcctgatat gcgcgatcag accctcttcc ccgagccgca
     2221 ccatcgtctc cggggcatcg gcgacgcgga agagcgcttg tgtagccttg ttgacgccct
     2281 tgtccgtcga ttgggcggac agaacgaccg ctatcagcaa cgtgaaggga ttggagtaga
     2341 cgagttccgt tttcggctcc ggcatctgtt gcttcagccg ttcaaagaac gtctcgcact
     2401 cttttttcga aaatggcttc gccaagcctg ctgcctcgcg aaaagtttcg ccacggcatt
     2461 gcctcatttg tctttcttcc gcaatccgca ccgcccgaca ggtcccggcc tccttgtcca
     2521 tctctgcaca aaagcgcatc ctcaagccat gagcgcggat aaccatcccg ttcttttcga
     2581 ccagacgctg cggcccaatc cgcccatgaa cattggcgcg cttgtttttg tcgccactgc
     2641 cgtcgcaact atcaatctgg cattcggact ctattttgtt ttgcgcggcg catggcccgt
     2701 gacgccgttc atgggtctgg acgcgatcct gctggcctgg gcctttcgcg caagcttccg
     2761 tacagcaagg tgttacgagc ggatttgtct gacgcccacg atgctgcggg tcgagcacca
     2821 tcctgcgcgc gggcgtccaa tccgttccga gttcaacccc tattgggtgc gtgtcgattt
     2881 cggggaagcc cccggcaggg gacgcagtct cgctctggca agccatggcc tgttcgtgca
     2941 gatcggtgca ttccttcctc cggcccaaaa actttcggtt gcccagggtc tcacggcggc
     3001 gctggcaaga tcgcgccaaa ccaatttcga ctgaccgcaa gattcgggtc gcaaagcgat
     3061 ttcggataac ccatcttccc atgatgaaca tgcacgctat cgatcacgag ccggccgagg
     3121 attcttcgca ctatcgcagg atcgcccgcg cggtccggtt cctggcggaa aattatcgcg
     3181 atcagccgac gctcgaagaa gccgccggcg ccgccggtct ttcgcccttt cattttcaac
     3241 gtctattcag ccggcatgtt ggagtgagcc cgaagtcgtt cgtcgcaaac ctcacgctcg
     3301 ggcatgccaa gacgaggctg gcgcagggcg caagcgtgct cgaggctgct ctggactccg
     3361 gactttccgg tccgtcgcgc cttcacgacc tctgtctcaa aattgaggcc atgacgcccg
     3421 gggaatttgc aagagaagga cgcgggctgc atatcgccca tgggttctgc gattcgcctt
     3481 tcggacgtac gcttgtcatg tccaccgtgc agggtgtttg cggtctggct tttgcagacg
     3541 aagaggaaat gccagaagca ctgaccgcca tgaagtcgcg atggccgcag gccgaatatt
     3601 ttgaggatgc gccttacatc gaggtcctcg gcgagaagat cttcgccccg caacctggcg
     3661 gtcatctgac gctgcatctg atcggcacgc gctggcagat caaggtctgg caggcgctgc
     3721 ttgcgatacc gccgggcacg gttgtcagct atcgcacgat tgcacaacat gttcgcaacg
     3781 cacacgacgc ccgtgcggta ggcggcgccg tcggaagcaa tcccatcgcg tggctgatcc
     3841 cgtgccatcg cgtgctcgcc agcgatggaa cgcttcacgg ctatcattgg ggcctggcgc
     3901 gaaagcgcag catgctggcc gttgaagcgg cgcgaaaggc ggcgtagggc cgtcgccggc
     3961 gccagttcct acccggccaa gccgagcaca tcggccattg tgtagagccc gggcttctgc
     4021 cctcgcgccc attttgccgc cgcgagcgca ccgcgcgcga aaatctcgcg atcctcggcg
     4081 acgtgttcga gaacaacccg ctcttgaggg cccgcgagta tcaccttgtg ctcccccgcc
     4141 atcgtgccgc cacgcaggga ggcgaaaccg atcgaaccgt ctttgcgcgc gcctgtgcgt
     4201 ccggtgcgtg ggggcgcgac atgcgttccg agatcaacac cgcggccctc cgctgcggca
     4261 cggcccagaa gcaatgccgt acccgacggc gcatccacct tcatccgatg atgcatttcg
     4321 acgatctcga catcgaagtc gggcaatgcc ctggcggcgc gtttgacgag tgccgcgagc
     4381 aaagcgatgc cgaggctcat attgccggac ttgacgatca ccgcctcgcg cgccacgact
     4441 ctgatgcgct cttcctcggc ggtcgagaat cctgtcgtgc cgatcacgtg cgcgatgcgt
     4501 gcctgcgccg aaagcgcggc gagggccacc gagacggaag gcgtggtgaa atctatgagc
     4561 gcgtcggctc ccgccagcgc ggcgcgcgca tcttccgcga ccgggatatc tgccggggga
     4621 agatgaacca tccgtccggc atcggttccg agatcgggat gagcgggcgc ttcgagagcg
     4681 ccggccagcg aaaacgcg
//
LOCUS       DASZMC010000543         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_790444,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000543 DASZMC010000000
VERSION     DASZMC010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790444"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VG274_11100"
     CDS             <1..346
                     /locus_tag="VG274_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07248.1"
                     /translation="EASALGITQLLDKPALAKLYRNPFGSSRTPDFVAITKHGLIYTS
                     GTKLAEHGGFAADDRNVALVVSNPSIGAATNNANVETRQIASTVLEVLGINPRELEGA
                     RKENTKPLPGGY"
     gene            complement(622..>2635)
                     /locus_tag="VG274_11105"
     CDS             complement(622..>2635)
                     /locus_tag="VG274_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWE07249.1"
                     /translation="QGIKTAIIQVAAEELDLLPDQISLVTADTAQTPDEGFTAGSHSM
                     QGSATAVMNAAAQARALLIAEAARQWNLRPASLSARDGVVAAPDGRKLTYGALAQLAS
                     LHVAAAPSSPLKAPRDHELIGKSLPRVDIPEKLTGGTAFVQDLRLPDMVHARVVRPPG
                     YGARLVSADIDAAARMPGVLKLVRQGSYLAIVAAREWEAIQAARALHASAKWTPPLPL
                     PDQARLHATLEQFHAENVRVYETRAAAAPSVRTLKARYTRPYIMHGAIGPSCAVARFE
                     NKALTVWTHSQGVFPLSRALSELLHMPQEAIRCIHMEGAGCYGHNGADDAAADAALIA
                     RELPGKPVRVQWMRDDEHRWEPYGPAMIVETSASLGADGRVVDWRHAVWSNTHTRRPT
                     AGGLFLQNAVLPQPLPAPPAKPIPMPEGGGDRNSIPLYAFANAEIVYHFIPEMPLRVS
                     ALRSLGGQMNVFAIESFMDELARAASIDPVAFRLKHLADPRAIAVIHAAASQFGWTHW
                     RPRGPLHGKGFAFARYKNLAAYCAIALDLSVEPETGAVQLGRVIAAVDSGEAVNPDGI
                     RNQIEGAIVQAASWTLFEQVGFDGTHITSTDWNSYPILRFPAAPQSKEVIVIDRPGEP
                     FLGSGEAGQGPACAAIANALADAIHNRLRDLPFSAARVKEAMRRRS"
BASE COUNT          444 a          889 c          881 g          421 t
ORIGIN      
        1 cgaggcctcc gcgctcggca tcacccagct gctcgacaag cccgcgctcg cgaagctcta
       61 tcgcaacccg ttcggcagca gccgcacgcc cgacttcgtc gccatcacca aacacggcct
      121 gatctatacg agcgggacga aactcgccga gcatggcgga ttcgcggccg acgaccgcaa
      181 cgtcgcgctg gtcgtttcca acccgtcgat cggcgcggcc accaacaatg caaatgtcga
      241 gacgcgccag atcgcgtcca ccgttcttga ggtcctcggc atcaatccca gggagctcga
      301 aggcgcccgc aaggaaaaca ccaagccgct tcccggcggc tattgatccg cctcggcggc
      361 cgctccccac cggagccgcc gttctttttc ctcccccacg cattggcgtg ggggaggtgc
      421 cccccaccgg tccgcgcgaa gcgcggccgg aggacaagct ccggggcgga gggggcaagc
      481 ggcagacgcc ccacttaccc ccgccggcgc ttcccccggc ccaaggccgg cggaagcgcc
      541 acctcccccg cgaaagtgcg ggggaggata aatgagcgga aaatcatctt cgggcgcgga
      601 gatcgaccgg tccactcctt ctcagctgcg cctgcgcatt gcctccttca cgcgcgctgc
      661 ggagaagggc aggtcgcgca ggcggttgtg tatggcgtcg gcgagcgcgt tggcgattgc
      721 tgcgcaggcg ggcccctggc ccgcttcgcc ggagccgagg aacggttcgc cgggccgatc
      781 gatgacgatc acttccttgg attgcggcgc cgcgggaaag cgcaggatcg gataggagtt
      841 ccagtcggtg ctcgtgatgt gcgtgccgtc gaacccgacc tgctcgaaaa gcgtccagct
      901 cgcggcctgc acgatggcgc cctcgatctg gttcctgatt ccgtcgggat tgacggcttc
      961 gccggaatcg accgcagcaa tcacgcggcc gagctggacc gcgccggtct cgggctcgac
     1021 ggaaagatcg agtgcaatcg cgcaatacgc cgcgaggttc ttgtagcggg cgaaggcgaa
     1081 gcctttgccg tggagcggac cgcgcggccg ccaatgcgtc cagccgaatt gcgacgcggc
     1141 tgcatggatg acggcgatcg cacgcggatc ggcgagatgc ttcagccgga atgcgaccgg
     1201 atcgatcgaa gccgcgcgcg cgagttcgtc catgaagctc tcgatggcga acacgttcat
     1261 ctgcccgcca agcgagcgca gcgccgagac gcgcaacggc atctccggaa tgaagtgata
     1321 aacgatctct gcattcgcaa aggcgtaaag cggaatgctg ttgcgatcgc cgccgccttc
     1381 cggcatcggg atgggtttgg cgggcggcgc cggcaatggc tggggcagaa cggcattctg
     1441 caagaagaga ccgccggcgg tcggacgcct tgtgtgtgtg ttgctccaca ccgcgtgacg
     1501 ccagtccacg acccggccgt cggcgccgag cgaagcgctc gtctcgacga tcatcgccgg
     1561 accgtaaggc tcccagcggt gctcgtcgtc gcgcatccat tgcacgcgca cgggcttgcc
     1621 cggaagttca cgcgcgatga gcgccgcgtc tgccgccgca tcgtccgcgc cgttgtggcc
     1681 gtaacagcct gcaccctcca tgtggatgca ccggatcgcc tcttgaggca tgtgcagcag
     1741 ttcggagagc gcgcgactga gcgggaacac gccctgcgaa tgggtccaca ccgtcagcgc
     1801 cttgttctcg aaccgcgcca ccgcgcagga cggcccgatt gcgccgtgca tgatgtaggg
     1861 gcgcgtgtag cgtgccttca gtgtacggac ggatggcgcc gctgcggccc tggtttcgta
     1921 gacgcgcaca ttttcagcgt gaaattgctc aagcgtggcg tgaagccgcg cctgatcggg
     1981 caagggcaac ggcggtgtcc atttcgccga cgcgtgaagg gctcgggcgg cctgaattgc
     2041 ctcccactcg cgcgcagcca caatcgcgag ataactgcct tggcgcacga gtttgagcac
     2101 gcccggcatg cgcgcagcag cgtcgatgtc ggcgctgacg agccgcgcgc catatccggg
     2161 cggccgcacg acgcgcgcat gaaccatgtc gggcaggcgc agatcctgca cgaaggccgt
     2221 gcccccggtc agcttctccg gaatatcgac gcgcggcagc gatttgccga tcagctcgtg
     2281 gtcgcgcggg gctttgagcg gcgaagacgg cgccgccgcg acatgcagcg acgcaagctg
     2341 cgccagcgca ccgtaggtga gcttgcgccc atcgggcgct gcgacgacgc cgtcgcgcgc
     2401 cgacaggctt gccggccgca ggttccattg gcgggccgct tccgcaatga gaagcgcacg
     2461 tgcctgcgcg gcggcgttca tcaccgcggt tgcgctgccc tgcatcgaat ggctgcccgc
     2521 ggtgaaacct tcgtcgggag tctgcgcggt gtccgcagta acgagcgaaa tttgatccgg
     2581 cagcaggtcg agctcctcgg cggcaacctg aatgatcgcc gtcttgatcc cctgc
//
LOCUS       DASZMC010000544         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_790678,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000544 DASZMC010000000
VERSION     DASZMC010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790678"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..>1919
                     /locus_tag="VG274_11110"
     CDS             121..>1919
                     /locus_tag="VG274_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE07250.1"
                     /translation="MISLRCFAIAVFAAWVAPLAAQDASSGYIGGAACGRCHASQFGS
                     QSKTGHAHALRRAQPTDPGPGSHAQWAFGAGAKATTWVSQTGEESIAEHGLTYYASTK
                     SLALTPGHTTSADITFRTFDPVGTALRCFRCHSTGPVMLAANFKVQPSEPGVHCEACH
                     GAGRAHAESGGKAAIQNPRRMTAGQINVLCGACHRQASDLDDETDWSNAWNVRHEPSY
                     LHRAACFRNSKGALSCITCHDPHQPLQTAAASYDAKCVSCHPKVAHAAPVASGSCAGC
                     HMPQVATSANLKFTNHWIGIYDSRGRKLTPSKRAVKDLRPASAGAESGDGIVVPGDAS
                     TLTPVYEKAVTERERTAGPESAKVARASSDLGSFLLEIRKSAEAEAPLRRAVSIDEHN
                     ADPAIDKDRESLALALQAEGRRDESFDLFKRAAEGNNPRVAARSLAKLAEMDSEHADV
                     YYKNAVAFEEKASGTGSPRVAVLLQEYALALRARDRDAEAEPVLRRALSIQQSSPKAD
                     PQVTIGVLNTLGNLLEGRRQFEQAEKLERAALALSEEKMGPESVQLAMTCTNLADVLW
                     NEKNLRGAGLLYRRAIAIDASLYGPDRPETAAD"
BASE COUNT          354 a          607 c          630 g          328 t
ORIGIN      
        1 atttgcgcgg gaacgtgtac agagctgtcg aaggccgcat ataatctcct ttcgcggagc
       61 tttcgccacg gcgaccaggg ccgctctggt ggagcttggc gaaaggcagc ggccgggccg
      121 atgatttcgc ttcgatgttt cgcgatcgct gtcttcgccg cctgggtggc gccgttggcc
      181 gctcaggatg ccagctcggg ctacatcggc ggcgcagcct gcggcaggtg ccacgcatcg
      241 cagttcggat cgcaatcgaa aacgggacac gcgcacgcgc tccggcgtgc gcagccgacc
      301 gaccccgggc cgggcagcca cgcgcaatgg gcgttcggcg ccggtgcgaa ggccaccacg
      361 tgggtgagcc agaccggcga ggaaagcatc gccgaacatg gcttgactta ttacgcttcg
      421 acgaaatcgt tggccctgac tcccggccat acgacctctg ccgatatcac ctttcgtact
      481 ttcgatccgg taggaacggc gttgcgctgt tttcgctgcc actcgaccgg gcccgtcatg
      541 ctggcagcga acttcaaagt acagccgagt gagccgggcg ttcattgcga ggcctgccac
      601 ggcgccgggc gcgcacacgc ggaatcgggc gggaaggcgg cgattcagaa tcccagacga
      661 atgacggcgg ggcagatcaa cgtcctctgc ggcgcatgcc atcgccaggc gagcgatctt
      721 gacgacgaga cggactggag caatgcctgg aatgtgcgcc atgagccttc ctatcttcat
      781 cgcgccgcct gttttcgcaa cagcaagggc gcgctttcct gcatcacgtg tcatgatccg
      841 catcagcctc tccagacggc ggcggcttct tacgacgcga aatgcgtctc ctgccatccc
      901 aaggtggcgc acgcggctcc ggtcgcgtcc ggcagttgcg cgggctgcca tatgccgcag
      961 gtggcaacca gcgcaaacct gaagttcacg aatcactgga tcggcattta cgactcgcgc
     1021 ggccgcaaac tgacgccttc gaagcgggct gtgaaggacc tgcgcccggc ttcggcaggc
     1081 gcggaatccg gggacggaat cgtcgtgccg ggggacgctt cgacgctcac gcccgtctat
     1141 gagaaggccg tcacggaaag ggagcgaacg gcggggccgg agagcgcgaa ggtcgcgcgc
     1201 gcctcgtcgg atctcgggtc atttcttctc gagatcagga agagcgcgga agccgaagcg
     1261 ccattgcggc gcgccgtttc gatcgacgaa cacaacgcgg atccggcaat cgataaggat
     1321 cgcgaaagcc ttgcgcttgc actccaggcg gaaggcaggc gcgatgaaag ttttgatctg
     1381 tttaagcggg cggcggaagg gaacaatccg cgcgtcgccg cgcgttctct cgcgaagctg
     1441 gccgagatgg attccgagca cgccgacgtt tattacaaga acgccgtcgc gttcgaggag
     1501 aaggcgtccg gaaccggctc gccacgcgtc gccgtccttt tgcaggaata tgcgctggca
     1561 ttgcgcgctc gcgaccgcga cgctgaagcg gagcccgtct tacgccgcgc gctttccatt
     1621 cagcagtcct ctcccaaagc cgatccgcag gtgaccatcg gcgttctgaa cacgctgggc
     1681 aacctgctcg agggacggcg ccagttcgag caggccgaga agctggagcg cgccgccctg
     1741 gcgctttcgg aggagaagat gggtccggag agcgttcaac tggccatgac ttgcaccaac
     1801 ctcgcggacg tcctctggaa tgaaaagaac ctccgcggcg ccgggctgct ttaccggcgc
     1861 gccatcgcca tcgacgcttc gctctatggt ccggaccggc cagaaacggc ggcggacat
//
LOCUS       DASZMC010000545         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1357944,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000545 DASZMC010000000
VERSION     DASZMC010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357944"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /gene="ssb"
                     /locus_tag="VG274_11115"
     CDS             complement(<1..191)
                     /gene="ssb"
                     /locus_tag="VG274_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWE07251.1"
                     /translation="MAGSINKVILVGNLGRDPETRRTTAGDPIVNFTLATSESWRDKQ
                     SGERRERTEWHRVVIFNEQ"
     gene            complement(336..2321)
                     /locus_tag="VG274_11120"
     CDS             complement(336..2321)
                     /locus_tag="VG274_11120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWE07252.1"
                     /translation="MIATNGTGRAGSTYRPEIDGLRALAILPVVLFHYRAPGFGGGFV
                     GVDVFFVISGYLITSLIQPEMDRGTFSLTRFYERRVRRIFPALFAMLAIVSLAALVFF
                     FPADLVRYAQSLFATALFAANFEFWREVGYFDVAANQKPLLHLWSIAVEEQFYLLYPA
                     LLLALRRVSPWHRMAAIAGVFLASFAVSVWGAGAAQAAAFYLLPARAWELMLGALLAL
                     GAVPDLKSRVASDGLALLGLVLIAGSVFGFTPQTPFPGFAALAPCLGAALVIYAARPD
                     RTIAGKLLAARPLVFVGLISYSLYLWHWPVFVFATYVAFREPSGVGSALLIALSVVLA
                     GISWRYVEQPLRRPLPGAPRRAPFAAAALVAGLTAASAALAASTDGLPQRLRPALQRI
                     LAEQDDHEPRIARCFGLTSEDVRKGRLCRIGAVGAVAPSFLLWGDSHADAILPAVAEA
                     AARVGRAGVFAGGEACPPLLGVTTPRPDCRAFNDAVAALAKRPEITEIVLESRWAKYA
                     EGSTYGEEPRGRVVLLDNSCRTAAPNDNHGVFLRGLDRTVSELRKEGKKVVIVASVPE
                     IGWPVPAVLARRALAEDAEGADPDAGTYLNRQRFALNAFASLRQRYGAIVLYPHSVLC
                     RTGSCEVALNGIPLYRDEHHLSVKGALQLLPLFTQTF"
BASE COUNT          496 a          848 c          825 g          393 t
ORIGIN      
        1 atctgctcgt tgaagatcac gacccgatgc cattccgtcc gttcccggcg ctcgccactc
       61 tgcttgtccc gccagctttc cgacgtcgcg agcgtgaaat tgacgatcgg gtcgcccgcg
      121 gtcgtacgcc gcgtctcggg atcgcgcccg agattgccca ccaggatcac cttgttgatg
      181 ctgccggcca tctgcattct ccactgatag agttccgtga cgttagccgc cccgccccca
      241 cccgcgccat cgcgcggctc ccgccggctt gtggacagaa cgactgggct tcttaccact
      301 gttcttgttg tgttccaata gcaattccag cgcggctaga acgtctgcgt gaacagtggc
      361 aggagctgga gggcgccctt cacgctcaga tgatgttcgt cgcgatagag cgggatgccg
      421 ttgagcgcca cttcgcagga ccccgtccgg caaaggacgc tgtgcggata gaggacgatc
      481 gcgccgtagc gctgccgcag actcgcgaag gcattcagcg caaacctttg ccggttcaga
      541 taggtgccgg catcgggatc ggccccttct gcatcctcgg ccagcgcgcg gcgcgcgagt
      601 accgcgggca ccggccagcc gatctcggga acggaggcga cgatgacgac cttcttgccc
      661 tccttccgaa gctcactgac cgtccggtcg agtccgcgga gaaatacgcc gtgattgtcg
      721 ttgggagcgg cggtgcggca ggaattgtcg agaagcacga cgcggcctct cggttcctct
      781 ccgtaggtcg agccttctgc gtatttcgcc caacgcgatt ccaggacgat ctcggtgatt
      841 tccggccgct tcgccagcgc ggccaccgcg tcgttgaagg cgcggcaatc tggcctgggt
      901 gtggtgacgc cgagaagcgg cggacaggct tcgccgcccg cgaacacgcc agcgcggccg
      961 acacgcgcgg ccgcctctgc gacagcaggc agaatcgcgt cggcgtggga atcgccccac
     1021 aggagaaagc ttggtgcaac ggcgcccacc gcgccgatgc ggcacagacg gcccttgcgc
     1081 acgtcttcgg atgtcagccc gaaacagcgc gcgatgcgcg gttcgtggtc gtcctgttcg
     1141 gccagaatgc gctgcaaggc cggccgcaga cgctgtggca atccgtcggt cgacgccgcc
     1201 agtgccgccg aagcggcggt gagtccggcc acaagagccg cggcggcgaa cggagcgcgg
     1261 cgcggcgcgc ccggcaaagg cctgcgcaac ggctgctcca cgtaacgcca tgagatcccc
     1321 gccaacacaa cggacagcgc aatgagcaag gcgctgccca cgcccgatgg ttcgcgaaag
     1381 gcgacatagg tcgcgaagac gaagaccggc cagtgccaca gatagaggga ataggagatc
     1441 agcccgacga acacgagcgg gcgcgccgcc agcaattttc cggcgatggt cctgtccgga
     1501 cgcgccgcgt agatgaccag cgccgcgcca agacatggcg caagcgccgc gaatcccggg
     1561 aacggcgtct gtggcgtgaa gccaaaaacc gatcccgcga tcaggaccag cccgagcaag
     1621 gccaacccgt cggacgccac cctggatttc aggtccggca ccgcgcccag ggcgagaagc
     1681 gcgcccagca tcagctccca ggcgcgcgcc ggaagaagat agaacgccgc ggcctgcgcc
     1741 gcgccggcgc cccagacgct cactgcgaag gatgccagga aaacgcccgc gatcgcggcc
     1801 atgcggtgcc atggagagac gcggcgcagc gcgaggagga gtgccggata gagaaggtag
     1861 aactgttcct cgaccgcaat cgaccaaaga tgcagaagcg gcttttgatt cgcggcgacg
     1921 tcgaaatagc ccacctcgcg ccagaattcg aaattggccg cgaacagtgc ggtcgcgaac
     1981 aggctctgcg catagcgcac gagatctgcc gggaagaaga agacaagggc agccagcgag
     2041 acgatcgcca acatggcgaa cagggcgggg aaaatccgcc gcacccgcct ttcgtaaaat
     2101 cgcgtgagcg agaatgtgcc ccgatccatt tcgggctgga tcagcgacgt gatcagataa
     2161 cccgagatca cgaagaacac gtcgacaccg acgaacccgc caccgaagcc gggcgcgcga
     2221 tagtggaaga gcacgaccgg caggatcgcg agcgcccgca gcccgtcgat ttccgggcgg
     2281 tacgtggagc ctgctcggcc ggttccgttc gttgcgatca tcgaccttta cccggactca
     2341 aaactgagtg atgcattttc gcggcccgcc gcaagacttt aacgcgaagg acgctgtggt
     2401 tgcgcggagg gcgctgtggt ttatttgcgc gcgtagcgcg ccatgagtgc cctctgcgtc
     2461 ccccgcgctt ccaccgcgac cgctgcgaaa aacttctgtc tcggcggcca cccgttgcat
     2521 atcgcgcccg ttctacctac gttccaaagg gaatcgtggg cc
//
LOCUS       DASZMC010000546         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4358977,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000546 DASZMC010000000
VERSION     DASZMC010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4358977"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="VG274_11125"
     CDS             complement(<1..1949)
                     /locus_tag="VG274_11125"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HWE07253.1"
                     /translation="MNPVSVKSGLFRDRREAGRVLAEKLATYANRSDLLVLALPRGGV
                     PVAYEVARALRAPLDVYVVRKLGVPGYEELAMGAVATGGVRVLNDQIVERLGIPDQMI
                     DAVAAREQRELARRERLYRGDRPPPHVNGRTVILVDDGLATGATMQAAVEALRQQNPA
                     RIVVAVPTASPDTCEEMKKKADEVICAITPEPFYAVGRWYDDFSQTTDDEVTALLGER
                     TTSEQGKSRHGSTADTPLIKALRESAYPLAGSVRDYDPLIGRIGEARFALLGEASHGT
                     HEFYCERAEITKRLITERKFTAVAVEADWPDAYRVNRYVRGVSDDVDAVEALADFRRF
                     PTWMWRNTVVVEFIEWLRAHNDALPLGVEKVGFYGLDLYSLHASMKAVLRYLEKVDPE
                     AAGLARERYSCFDHVGENSQAYGLMTRLKIAKSCEEEVIAQLVELQRRASDYMRRGGR
                     LAEDAQFYAEQNARLVKNAEAYYRSMYLEEVSSWNLRDRHMAETLDALAGHLGREGRL
                     AKIAVWEHNSHLGDARATDMSQRGELNVGQLAREKYHSEAVLVGFTTYHGTVTAAADW
                     DSPAERKRVRPALPGSYEALFHATQPDRFLLIWNDNHTVTEQLKFPRLERAIGVIYRP
                     ETERQSHYFQARLADQFDAVLHFDET"
     gene            complement(2087..>2920)
                     /locus_tag="VG274_11130"
     CDS             complement(2087..>2920)
                     /locus_tag="VG274_11130"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWE07254.1"
                     /translation="QAQKFGAKMMIPTEVRGLDLSGTPFAVHLDDGRRMKASTVVVAT
                     GARYRRLDVSNLEEFEGRGVWYWASPIEARLCREEEIVLVGGGNSAGQAAVFLRSFAA
                     KIWMLVRGPSLARSMSQYLIDRIRAVDNIEVLTHTEIVALYGSREKQLERVRWRNNVT
                     GEETEKPIRHVFLFIGAEPATTWLRGSGVALDTKNFVLTGPDIPFDARRSNNGSSRPL
                     ALETSVRGVFASGDVRSGSVKRVGAAIGEGAVTGAELHVALPNGTAALSRDAQNTKRD
                     S"
BASE COUNT          544 a          953 c          840 g          583 t
ORIGIN      
        1 gtctcgtcga aatgcagcac ggcgtcgaat tggtcggcta ggcgcgcctg gaaataatgg
       61 ctctgccgct cggtttcggg ccggtagatc accccgatcg cccgctcgag ccgagggaat
      121 ttcaattgct ccgtcaccgt atggttgtca ttccagatca gcaggaaacg atcaggctgc
      181 gtggcgtgga acagcgcctc atagctgccc ggcagcgccg gacgaacgcg cttacgctcg
      241 gctgggctgt cccaatcggc ggcagccgtg acagtaccgt ggtacgtggt gaagccgacc
      301 agcaccgctt cgctatgata tttttcacgg gcgagctgtc cgacattcag ctcgccacgc
      361 tggctcatgt cagtcgcccg tgcatcgccg agatgagaat tgtgttccca gacggcgatc
      421 ttcgcaagtc gtccttcgcg accaagatgt ccagccagcg cgtcaagtgt ctccgccatg
      481 tgccggtcgc gtagattcca tgacgacacc tcctcgaggt acatggatcg ataatacgcc
      541 tccgcattct tcaccaggcg ggcattctgc tcggcgtaaa actgcgcatc ctcggcgagc
      601 ctgccaccgc gccgcatata gtcggatgcc cggcgttgca gttcgaccag ttgagcgatg
      661 acttcctcct cacacgattt cgcgatcttc aatcgcgtca tgaggccata ggcctggcta
      721 ttctcgccga cgtgatcgaa gcaggagtaa cgctcgcgcg ccaatccagc ggcctccgga
      781 tccacctttt ccagatagcg caacacggcc ttcatcgatg catgcagact gtagaggtcg
      841 aggccgtaaa agccgacttt ttcgacgccc aacggcagcg cgtcgttgtg cgcccgcagc
      901 cattcgatga actcgaccac taccgtgttg cgccacatcc acgtcggaaa acgccgaaaa
      961 tcggcgagtg cttccacggc atcgacgtcg tcgcttactc cgcgaacgta gcggttgact
     1021 cgataggcat cgggccaatc tgcttcgacc gcgacggcgg tgaatttccg ttccgtgatg
     1081 agccgcttgg tgatctcggc gcgctcgcaa tagaactcgt gcgtcccatg ggacgcttcg
     1141 cctagtagcg cgaaccgcgc ttcgccgatg cggccgatca gcggatcgta gtcgcggaca
     1201 gatcccgcca gtgggtaagc gctttcacgc aacgccttga tgagtggcgt gtccgctgtc
     1261 gaaccgtgcc tgctcttccc ctgctctgac gtggttctct cgccgagcag cgccgtcacc
     1321 tcgtcgtcag tcgtctgcga aaagtcatca taccagcgcc cgaccgcgta aaatggctcc
     1381 ggtgtgatag cgcagataac ctcatccgcc ttctttttca tctcctcgca cgtatcgggt
     1441 gacgcggtag gaaccgcgac gacgatgcgc gccgggttct gctgccgcaa tgcctcaact
     1501 gccgcctgca tggtggcgcc ggtcgccagg ccatcgtcga caagaatcac ggtgcggccg
     1561 ttgacatgag gcggcgggcg gtcgccgcga taaagtcgct cgcgccgtgc cagttctcgc
     1621 tgttcgcgcg ccgccactgc gtcgatcatc tgatcgggga taccgagacg ttcaacgatt
     1681 tgatcgttga gcacgcgcac gccaccggtc gcgacggcgc ccatcgccag ctcttcgtag
     1741 ccgggaacac caagtttgcg gactacataa acgtccagcg gtgcacgaag cgcgcgggcc
     1801 acttcatacg caaccggcac gccgccgcgc ggcagagcca gcacgagcag gtccgaacga
     1861 ttggcataag ttgcgagttt ttcggcgagc acgcggcctg cttcgcggcg atcgcggaag
     1921 agtcctgact tgacgcttac cgggttcatt gctcgtcctc ctccctcatc gaggcaggct
     1981 ccaacggagt tgctcaaagc ttctcactga gcaagacgat gtgccatttg agccgcttca
     2041 cgaccatgac agccatcaat tgcgagagcc gccgttcgtc gatcatctag ctatcgcgct
     2101 tggtgttttg tgcatcgcgg gacaatgccg ccgtaccgtt cggaagagcg acgtgaagct
     2161 ccgctccggt gaccgcgcct tccccgattg cggcacctac ccgcttcact gatccggacc
     2221 gcacatcgcc ggaggcgaat acaccacgca cgctggtttc cagcgccaac gggcggctcg
     2281 atccattgtt cgatcggcgc gcgtcgaacg ggatatccgg gcccgtcaaa acaaaattct
     2341 tggtgtccaa cgcgactccg cttcccctga gccaggtcgt cgccggctcc gcgccaatga
     2401 acaggaagac atggcggatt ggcttttccg tttcctcacc ggtcacattg ttgcgccagc
     2461 gaacgcgctc cagctgcttt tcgcggctcc cgtacagggc aacgatttcc gtgtgcgtca
     2521 acacctcaat gttgtcgacc gcacggatac ggtcgatgag atattgcgac atgctccgtg
     2581 caaggcttgg cccgcgcacc agcatccaga tctttgccgc gaagctccgc aggaaaacgg
     2641 cggcttgacc agctgagttg ccgccgccga ccagcacgat ctcctcctcc cggcacaggc
     2701 gcgcttcgat gggcgaagcc cagtaccaga cgccgcgtcc ctcgaattcc tcgaggttgg
     2761 aaacatcaag acgtcgatag cgggcgccgg tcgcgacgac cacggtcgag gccttcatgc
     2821 gccggccatc atcgaggtga accgcaaagg gcgttccaga gagatcgagg cctctcacct
     2881 cggtggggat catcattttc gcaccgaatt tctgcgcctg
//
LOCUS       DASZMC010000547         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2139336,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000547 DASZMC010000000
VERSION     DASZMC010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139336"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2694)
                     /locus_tag="VG274_11135"
     CDS             complement(<1..2694)
                     /locus_tag="VG274_11135"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWE07255.1"
                     /translation="MPSLSREELDRREALKLLAAAMAATMTRCSRPDEEIIPYVDMPE
                     GLVAGEPMRFATTLSLDGYGRGFLGITVDGRPIKIEGNPRHPFSLGATDIFAESEVLS
                     LYDPDRSKTAMRKNRIATWDEFAANWNSVVGTASGHGLALVTGRIVSPTTLAQIDTLK
                     KRFPDLRWYRYEPVNDDSSRAGARLAFGRVVTALPRWAEADIILSLDADPLGHGPDEL
                     RIARAYASRRGSNRKRQRLHVVEPAWTLTGAAADFHLALLPGEIREIARAVANGLGAG
                     LGESKLPQHAGDFVKRVGSEIAAAPGRALVLAGEGQPPELHALCHWINAKIKAPVDYL
                     PPMDPVTSDHAEELVAFSRDLHAGRITALIVADANPVYDAPDSLHIADGIAAAPFSAH
                     AGLYADETADRCTWQLPMSHALEAWGDMRALDGTASLVQPLIRRLYDTRTTDEFLALL
                     CGQTGSNAFGLTQATWRNPFNADFETKWRTALHDGVIANSAAAPLHAPVSKAPNVPYA
                     ASARNLTLVMSPDSSVRDGRFANNAWLQECAKPFTRQVWGNALQISAGDAARLGIHDQ
                     DTIDLELGGATIRAPAILMQGQAEGVVAATWGQGRVKSGRIGTAIGTRVCGAGSLPFA
                     RALEGGTIRRAARAIRFRSAQAHTRLNGRAEDLFQIASLSGLTAEGRETERPAPSLLN
                     IPAKGEYAWAMVVDTDSCIGCNACVVACQAENNVPVVGPEEISQGRDMHWLRVDSYLR
                     PQAAAMRRGFEPVPCMHCELAPCEPVCPVEASVHDHEGLNVQVYNRCIGTRFCEANCP
                     YKVRRFNWFDYAGDQAYAGEGSALKAQHNPDVSVRGRGVMEKCNYCLQRISRARKTAE
                     KESREIAEGEMITACQAACPTRAIHFGDLNGTGSDVSRLRRA"
     gene            complement(2682..>3210)
                     /locus_tag="VG274_11140"
     CDS             complement(2682..>3210)
                     /locus_tag="VG274_11140"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWE07256.1"
                     /translation="HLTRAQPVPFSHEHHVGALGLDCRMCHTGVEKAAFAGIPTTHIC
                     MTCHSQIWTNAQMLAPVRASLASGRPLHWLRVNRVPDYVFFDHSIHVAKGIGCSVCHG
                     PVQKMALMRQEQPLTMGWCLECHRHPDLYIRPRNEVFNMFWSVPPDQIAQGRKLIAEY
                     LIHTAHLTDCSTCHR"
BASE COUNT          577 a          999 c         1040 g          594 t
ORIGIN      
        1 cgcgcgccgc agtcttgaaa cgtcggaccc ggttccattc aaatccccga aatggattgc
       61 ccgtgttggg cacgcggcct ggcaggcggt gatcatttcg ccttcggcga tctcgcggct
      121 ctccttctcc gccgttttgc gcgcgcggct gatgcgttga aggcagtagt tgcacttctc
      181 catcacgccg cggccgcgca ccgaaacgtc gggattatgc tgcgccttga gcgcggagcc
      241 ttcgccggca taagcctggt ctcccgcata atcgaaccaa ttgaagcgcc ggaccttgta
      301 aggacaattg gcctcgcaaa aacgcgtacc gatgcagcgg ttgtagacct ggacgttgag
      361 accttcgtgg tcgtgcaccg acgcttcgac cgggcagacg ggctcgcatg gggcaagctc
      421 gcaatgcatg catggtacgg gttcgaatcc gcggcgcatc gcagcggcct gcggacgcag
      481 ataggaatcc acccgcagcc agtgcatgtc gcggccctgc gaaatctctt ccggccccac
      541 aaccggcacg ttgttctctg cctggcatgc gacgacgcag gcattgcatc caatgcagga
      601 gtcggtgtcc acgaccatcg cccaggcgta ttcgcctttg gcggggatat tgaggaggct
      661 tggagccggt cgctccgttt cacgcccttc tgcggtcagg ccggaaaggc tcgcgatctg
      721 gaagaggtct tcggcgcgtc cgttcagccg cgtgtgagcc tgagcggagc ggaagcgtat
      781 cgcccgggct gcccttctga tcgttccccc ctccaatgcg cgcgcgaaag gaagcgagcc
      841 ggcaccgcag acgcgcgtcc cgattgctgt tccgatgcgc ccggatttga cgcgaccttg
      901 tccccacgtc gccgccacga caccctcggc ctgcccctgc atgaggatgg cgggcgctct
      961 gatcgtagcg ccgccgagtt cgagatcgat cgtgtcctgg tcatggatcc ccaaccgtgc
     1021 ggcatcgcca gccgagatct gcagagcgtt tccccaaacc tgccgggtga acggtttggc
     1081 gcattcctgc agccaggcgt tgttggcgaa acgtccatct cttaccgacg aatcgggaga
     1141 catgacgagc gtcaaattgc gggcagaggc ggcatagggc acgttcggcg ctttggagac
     1201 cggcgcgtgc agcggcgcgg ccgcagaatt cgcgatcacc ccgtcatgca gggccgttcg
     1261 ccacttcgtc tcgaaatcgg cgttgaacgg gtttcgccag gtggcctgcg tgaggccgaa
     1321 agcattgctg ccggtctggc cgcagaggag cgcgagaaac tcgtccgtcg tgcgtgtgtc
     1381 gtagaggcgg cgaatgagcg gctgaaccag cgaagcggtg ccgtcaagcg cgcgcatatc
     1441 accccacgct tcaagcgcgt ggctcatcgg caactgccat gtgcaacggt ctgcggtttc
     1501 gtcggcgtaa aggccggcat gagcgctgaa tggcgcggcg gcaatgccgt cagcaatgtg
     1561 cagtgagtcc ggtgcgtcgt ataccggatt ggcatccgca actatcaacg ccgtaatgcg
     1621 tccggcatgc aggtcgcgcg aaaatgcgac aagttcttcg gcgtgatcgc ttgtcaccgg
     1681 atccatcggc ggcagataat cgacgggcgc tttgattttg gcgtttatcc agtggcagag
     1741 cgcatgcagt tccggcggct ggccttcgcc tgcgagcacg agagcgcgtc cgggagcggc
     1801 ggcgatttcg ctgccgacgc gcttgacgaa atcgcccgcg tgttggggca atttcgactc
     1861 cccaagcccc gcgcccaggc cgttggcgac cgcgcgggca atctcgcgaa tctcccccgg
     1921 cagcaacgca aggtggaaat cggctgcggc gcccgttagc gtccaggcgg gttcgacaac
     1981 atgaagacgc tgccgcttcc ggttgctgcc gcgccgggac gcataggccc gcgcgatccg
     2041 caattcgtcg ggtccgtgcc ccagaggatc ggcgtcgagt gagaggatga tgtccgcctc
     2101 ggcccagcgc ggcaaagccg tcacaacccg cccgaaggcg agccgcgcgc cggcacgcga
     2161 ggaatcgtcg tttacgggct cgtagcgata ccaccgcagg tcgggaaacc gtttcttcaa
     2221 tgtgtcgatc tgcgccagcg tcgtcggcga gacaatgcgg ccggtaacaa gtgcaaggcc
     2281 gtggccggac gccgttccca ccaccgagtt ccagttcgcc gcaaattcgt cccatgtcgc
     2341 aatgcggttt tttcgcatcg cggttttcga gcggtcggga tcgtaaagcg acaacacttc
     2401 ggattcggcg aagatatcgg ttgcgcccag actgaacgga tggcgcggat tgccttcgat
     2461 cttgatcggc cggccatcga ccgtgatgcc gagaaagccg cgaccgtaac catcgagcga
     2521 caaagtcgtg gcaaagcgca tgggctcgcc cgcgaccaaa ccttccggca tatcgacata
     2581 ggggatgatc tcctcgtcgg ggcggctgca gcgcgtcatc gtcgcggcca tcgcggccgc
     2641 cagcagcttc agcgcctcac gccggtcgag ttcttcgcga ctcagcgatg gcatgtggag
     2701 caatccgtca gatgcgcggt gtggatcaga tattcggcga tgagcttgcg cccctgcgcg
     2761 atctggtcgg gtggcacgct ccagaacatg ttgaacactt catttctggg gcggatgtag
     2821 aggtccggat ggcgatggca ttcgaggcac cagcccatcg tgagcggttg ctcctgacgc
     2881 atgagcgcca tcttctgcac cggcccgtgg cacacggagc acccgatgcc tttggcgaca
     2941 tggatgctgt gatcgaagaa aacgtaatcg ggcacgcgat tgacacggag ccaatgaagc
     3001 ggcctgccgc tcgcaaggct cgcgcggacc ggcgccagca tctgagcgtt ggtccagatc
     3061 tgggagtgac aggtcatgca gatgtgggtg gtgggaattc cggcgaaagc ggccttctcc
     3121 acaccggtat gacacatgcg gcagtcgagc cccagtgcgc ccacatggtg ctcatgactg
     3181 aaagggacgg gctgagcacg cgtcagatgc
//
LOCUS       DASZMC010000548         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4898735,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000548 DASZMC010000000
VERSION     DASZMC010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4898735"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VG274_11145"
     CDS             <1..679
                     /locus_tag="VG274_11145"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HWE07257.1"
                     /translation="TDVDNQRAGMLPFVFENGFGYERYVDYALDVPMYFVYRDGRYID
                     MAGKSFRDFLARRVPDLKDIEPSLSDWADHLTTIFPEARLKHFLEMRGADGGTWRRIC
                     GLPALWVGILYDSTALDAAWDLVKDWTREEREAMRAAVPVLAFNTPFRGRTLRDLARD
                     MLEMSQAGLRSRAKEDAIGTTEEGFLQPLNDLVERGYTRAEELLRKWRTEWNGDLSRL
                     FTEYNFL"
     gene            complement(733..2028)
                     /locus_tag="VG274_11150"
     CDS             complement(733..2028)
                     /locus_tag="VG274_11150"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWE07258.1"
                     /translation="MVTESTLPPANANVVIIGAGFAGIEVARALGATNIPAILLDRQN
                     FHLFQPLLYQVATAALSPADIAEPVRKMVRRYASVEVRLAEVIRIDHPARTIMLSTGE
                     RLAYRWLVLAAGATHSYFGRDEWMQVAPGVKTIEDARAVRARLLLSFEQAEASRDPDE
                     QRRLTTFAVVGGGPTGVEIAGSIAELARYALAKDFRRIHPGSAKVLLFEAGDRILTSF
                     PEPLAVYAEKQLARLGVEVRTKCPVGGISERWITASGEKIPIGLAIWAAGVEASPLGG
                     TLAVQMDKTGRIKVKPDLSVPGMEGVYIAGDLALVRDRKGRALPGLAQVAKQQGIYLG
                     RALVRKIKTGVDSAPFVFKNRGNTAIIGRHSAIFDFGWCRVRGWPAWLLWAVIHVYLL
                     VGFQHRLLVVVQWVWRYLTYERGARLILEPPCQSHADGT"
BASE COUNT          418 a          665 c          698 g          409 t
ORIGIN      
        1 gaccgacgtc gacaatcagc gcgcggggat gctgcccttc gtgttcgaaa atggattcgg
       61 ctacgagcgc tacgtcgact acgccctcga tgtgcccatg tacttcgtct atcgcgacgg
      121 gcgctacatc gatatggctg gcaagagctt ccgagatttc ctggcccggc gcgtcccgga
      181 tttgaaggac atcgagccat cgctctccga ctgggccgat catctcacca cgatctttcc
      241 cgaagcgcgg ctgaagcatt ttcttgaaat gcgcggcgcc gatggcggca cctggcgtcg
      301 catctgcggt ctgcccgccc tgtgggtcgg catcctgtac gattcgacgg ctctcgatgc
      361 cgcatgggat ctggtgaagg actggactcg cgaagagcgt gaggcgatgc gcgccgcggt
      421 tccggtgctg gcgttcaata cgccgttccg cggccggaca ttgcgcgatc tggcgcgcga
      481 catgctggag atgtcgcaag ccggtcttcg cagccgcgcg aaggaagatg cgatcggcac
      541 caccgaggaa ggattcctcc agccgctcaa cgatctcgtc gaacgcggct acacgcgcgc
      601 cgaggaattg ctccgcaaat ggcggaccga atggaacggc gatctctcga gattattcac
      661 ggagtacaat ttcctctgac atggatgctg cagcggtgcc actcgtgcgt gctcctgata
      721 agcgcgcgcg gattaagtgc catcggcatg tgactggcat ggcggttcaa gaataagccg
      781 ggcgcctcgc tcgtaggtga gatagcgcca gacccattgc acgaccacca gcagccggtg
      841 ctgaaagccc accagcagat agacgtggat caccgcccag agcagccagg ccggccagcc
      901 tctgacgcgg caccagccaa aatcgaatat ggccgaatga cgccctatga tcgcggtgtt
      961 gccgcggttc ttgaaaacga agggcgcgct gtccacgccg gttttgattt tcctgacgag
     1021 ggcgcggccc agatagattc cttgttgctt tgcgacctgc gccaggccag gcagcgctcg
     1081 ccccttcctg tcgcgcacga gggccagatc gcccgcaatg taaacgccct ccatgccggg
     1141 aaccgacaga tctggtttca ctttgatgcg tccggttttg tccatttgca ctgcaagcgt
     1201 gccgccgagc ggcgacgcct cgacgccagc cgcccagatt gcgaggccga ttggaatctt
     1261 ttcgccagac gcggtgatcc atcgttcgga aatgccgcca accgggcatt ttgtgcgcac
     1321 ctcgacgccg aggcgggcga gttgtttctc ggcgtagacc gccagaggtt ccggaaacga
     1381 ggttagaatc cggtcaccgg cttcgaagag aagtaccttt gcggagcctg gatggatgcg
     1441 gcgaaagtcc tttgcgagcg cgtaccgcgc cagttcggcg atcgaacctg cgatctcgac
     1501 accggtgggc ccgccgccga ccaccgcaaa ggtcgtcagg cggcgctgct cgtcggggtc
     1561 gcgggatgcc tccgcctgtt cgaagctcag caggagtctg gcgcgaaccg cgcgtgcgtc
     1621 ctcgatcgtc ttgacgcccg gcgcgacctg catccattcg tctctgccga aataggagtg
     1681 ggtcgcgcct gcagcgagga caagccatcg atatgcgaga cgctcgccgg tcgaaagcat
     1741 aatggtccgc gccggatgat cgatgcggat tacctcggcg agtctcacct ccaccgaggc
     1801 gtagcgccgg accatcttgc ggacgggctc ggcgatatcg gcaggcgaga gggccgccgt
     1861 ggcgacctgg tagagcaatg gctggaaaag gtggaaattt tgacgatcga gcaggatcgc
     1921 ggggatattc gtggcgccga gcgcgcgcgc cacttctatg cctgcaaagc cggccccgat
     1981 gatgacgaca ttcgcattgg cgggcggaag agttgattcg gtgaccattt ccgggatgct
     2041 gcaccataca ggcaacgacg gacagggcca tgaacatcat ggtgcggccg agcgtgaagc
     2101 tgccagcggg ctggaaattc ggctatttca gggcatacac cgccgagccg tcggatttca
     2161 ggaagtcata cgagcatgtc cgcaagctga
//
LOCUS       DASZMC010000549         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1865794,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000549 DASZMC010000000
VERSION     DASZMC010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865794"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VG274_11155"
     CDS             complement(<1..749)
                     /locus_tag="VG274_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWE07259.1"
                     /translation="MTLSSAATMSGNALSTVNVLIVNEGLSVLAGVLILIVGWLIATW
                     AKRLTARGLAHLPLDPTLKPLIASLMRYTILIFTIVLVLGQFGVQTTSLIAVLGAAGL
                     AIGLALQGTLSNVAAGVMLLLLRPFRVGHFVQAGGVSGTVREIGLFTTLMTTRDLIYV
                     SVPNSAVWSGVISNYTREPLRRIVFTLPVDIANDIDKVEKAILCALTKNEHVLKAPEP
                     WVGVSALQEYSVVMSVNCYVKSEDYWRSLPA"
     gene            864..1640
                     /locus_tag="VG274_11160"
     CDS             864..1640
                     /locus_tag="VG274_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWE07260.1"
                     /translation="MLKMSVWVLPFVMVAGSASAMPDCAGPLEITTAHIVRVEHTNDV
                     LVMRDGRAIHMEGIRIPHGSRDRAPAFIADQSYDAVNALVRGKTVDVTAVVPKEDRYD
                     RVRGQVINSDDQWLQMNLLKSGLARVDIAPDRTECAAELYAAEGAARATNTGLWAVPA
                     YRVRTPDTLGGDTGTFQIVQGKVLTADMKEGRVYLDFGADWKTDFTVTISPEDMANFR
                     AMDVDPRDYAGKTIRVRGIIQQFNGPEIEIANPKQVEVLQ"
BASE COUNT          366 a          542 c          565 g          328 t
ORIGIN      
        1 attgccggga gggagcgcca gtaatcttcg ctcttgacgt agcagttcac cgacatcacc
       61 acagaatatt cctgcaatgc cgagacgccg acccacggct cgggtgcctt cagcacgtgc
      121 tcgttttttg tcagcgcgca aaggatcgct ttctcgacct tgtcgatatc gttggcgatg
      181 tcgacgggca gcgtaaacac gatccggcgc agtggctcgc gcgtgtaatt gctgataacg
      241 ccgctccaca ctgcggagtt cgggacagag acgtaaatga gatcgcgcgt cgtcatcagc
      301 gttgtgaaca gccctatctc gcgcaccgtg ccgctgacgc cgccggcctg tacgaaatgg
      361 ccgacacgga atgggcgcag caacaacagc atcacgccgg cggcgacgtt ggagagcgtg
      421 ccctgcagcg cgaggccgat cgccaaaccc gccgcgccca ggacagcgat caggcttgtc
      481 gtctggacgc cgaactgacc gagcacgagg acgatcgtga agatcaggat ggtgtagcgc
      541 atcaaggatg cgatcagcgg cttgagcgtg gggtccagcg gcaggtgtgc gaggccgcgc
      601 gccgtcagcc gtttcgccca tgtcgcgatg agccacccga cgataaggat cagaacgccc
      661 gcaaggacgc tcaaaccctc attgacgatg agtacgttga cagtggagag cgcgttgccc
      721 gacatcgtgg ctgcgctgga cagagtcatg gcgtggttaa ccttcgtgac gttttacctg
      781 ttatggaaac gcgtcgcgcg ctaaatcggc catggtgcgc gaagccggat tttgcgcaca
      841 atcaggatgt tatgggggcg acgatgctga aaatgtccgt gtgggttctg ccgttcgtta
      901 tggtcgcggg ctcggcaagc gccatgcctg actgcgccgg gccgctggag atcacgaccg
      961 cacacatcgt tcgcgtcgag cataccaatg acgtcttggt gatgcgcgat ggccgcgcca
     1021 tccacatgga aggcatccgc attccgcacg gaagtcggga tcgcgcgccc gccttcatcg
     1081 ccgaccagtc ctacgatgcg gtcaatgcac tggtgcgcgg caagaccgtg gatgtaacgg
     1141 ccgtcgtgcc gaaagaggat cgctacgacc gcgtgcgcgg acaggtcatc aacagcgacg
     1201 accaatggct gcagatgaac ctgctgaaaa gcggtcttgc gcgcgtggat atcgcgccag
     1261 accgcaccga gtgtgcggcg gagctttatg cggccgaggg tgccgcgcgc gcgaccaaca
     1321 ctggactctg ggccgtgccc gcatatcgcg tccgtacgcc cgacacgctc ggcggcgaca
     1381 ccggcacgtt ccagatcgtg cagggcaagg tgcttaccgc cgatatgaag gagggccgcg
     1441 tctatctcga tttcggcgcc gactggaaga cagatttcac ggtgacgatc tcgcccgaag
     1501 atatggcgaa ctttcgcgcc atggatgtcg acccgcgcga ctacgccggc aagacgattc
     1561 gcgtgcgcgg tatcatccag cagttcaacg gccccgaaat cgaaatcgcg aaccccaagc
     1621 aggtcgaagt cctgcaataa cgccgcattc cggcccacaa atcgcgttca caacttccaa
     1681 ttgatacgct atcctgtccc cgggctcgtc ggatcggact ggggatcatg gatacattcc
     1741 ggaagtggtg gtatcacgaa acgacgtgga agcgctggct gtttcgcggc gccgcggtct
     1801 t
//
LOCUS       DASZMC010000550         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4082519,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000550 DASZMC010000000
VERSION     DASZMC010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4082519"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1031
                     /locus_tag="VG274_11165"
     CDS             222..1031
                     /locus_tag="VG274_11165"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWE07261.1"
                     /translation="MHDTWTAIALGIVEGLTEFLPVSSTGHLLVTEKLLQLDDNRWHA
                     FTIVIQLGAILSVVAVYWRKFWQVLVGLPSDPAARRFAAMIIVAFIPAGILGATLIKT
                     INTVLLNPSRALPVIGTAWILGGILILVLERIAPRPRYTQSDNLPLWKCVQIGFCQCL
                     ALLPGVSRSGATILGAELLGVERKAAAEFTFYLAVPTMLGASVFELYKEGATLTRHDE
                     SLIAIGFVVAFVVAYGVVRTFIAFIGRYGLKPFGWYRIASGLALFAFIWAH"
     gene            complement(1015..1980)
                     /locus_tag="VG274_11170"
     CDS             complement(1015..1980)
                     /locus_tag="VG274_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HWE07262.1"
                     /translation="MTRLVTVFGGSGFLGRYVVRALARAGYRIRVGVRRPNLGNYLLP
                     MGHVGQIQLVRTDVADADSVARAMKDAAAAINLVGVLYESGRQTFQRLHADSPATIAE
                     SARAEGVNALVHVSALGASGQSPSLYARTKAEGEMRLRETLPSAVILRPSIVFGPEDD
                     FFNRFAALARLLPALPLIGGGHTRFQPVYVGDTARAIATCMANQATQGRIYELGGPRV
                     YTFRELMQLVLAETRRRRFLIPLPFSFAMMQASILGLLPKPMLTRDQVLLLKQDNVVT
                     PGAATLADLGIEAETVEAIIPTYLWRFRREGQFEAPPREPVSAPR"
     gene            complement(2044..2328)
                     /locus_tag="VG274_11175"
     CDS             complement(2044..2328)
                     /locus_tag="VG274_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07263.1"
                     /translation="MLRNVLMLMCMAALGTGAVCLLLGADPPGFMLLIWGGILFAAVV
                     YERFRYKPLAQARPGPGWERTSERFVDAESGRTVTVYIQPASGERQYVEE"
     gene            complement(2318..2659)
                     /locus_tag="VG274_11180"
     CDS             complement(2318..2659)
                     /locus_tag="VG274_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HWE07264.1"
                     /translation="MIVATAEGVAGYRAKQTLGQCFGVVVRSRGLGGNIMAGLRSIVG
                     GEIHEYTQLLEEARRHAVDRLVRNATVMGANGVIMMRFDSAEIGQTMSEIVAYGTAVI
                     LEPVDAKTNAA"
     gene            3027..3111
                     /locus_tag="VG274_11185"
     tRNA            3027..3111
                     /locus_tag="VG274_11185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3061..3063,aa:Leu,seq:cag)
     gene            3283..3894
                     /locus_tag="VG274_11190"
     CDS             3283..3894
                     /locus_tag="VG274_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWE07265.1"
                     /translation="MKIKLHKGDLPDGLDLAPSVAIDTETLGLNPFRDRLCLVQLSSG
                     DDVCHAVQFAPGEYAAPNLKRMLGDPKVLKLFHFGRFDIGMLKRHLGVTAAPVYCTKI
                     ASRLARTYTDKHGLKDIVRELLGVELSKEQQSSDWGAPNLSERQLAYAANDVAYLHRL
                     KVALDAMLVREGRASHAEACFAFLPARVELDLAGWQDTDLFAH"
BASE COUNT          769 a         1371 c         1311 g          829 t
ORIGIN      
        1 gctaggacca agtttcgatc ctgcatgagt cgtgcaccag cagattttaa cgcagagggc
       61 gcggaggttt cgcggaggaa ttgcttgcgc gcttcgcgcg ccatcatgtt cctgcgccca
      121 ctgcgctgcc tctgcgccct ctgcgttaaa tctgcttttt gtgttactcg tcatcgactc
      181 gaaccaccag gcgttactcg ccgataacga cgggctcccg catgcacgac acctggactg
      241 ccatcgccct cggcatcgtc gagggattga cggagttcct gcccgtgtcc tcgaccgggc
      301 atctgctcgt cacggagaag ctgctccagc tcgacgacaa tcgatggcac gccttcacca
      361 tcgtcatcca gctcggcgcg attctctccg tcgtcgcggt ctattggcgg aagttctggc
      421 aggttctggt cggactgccg agcgatcctg cggcgcgccg ctttgccgcg atgatcatcg
      481 tcgccttcat tcccgccgga attctgggcg ccacgctgat caagacgatc aataccgtcc
      541 tgctcaatcc ttcgcgcgca cttccggtga tcggcacggc ctggatcttg ggcggcattc
      601 tcatcctcgt gctcgagcgc attgcgccca ggccgcgtta cacgcagagc gacaatcttc
      661 cgctgtggaa atgcgtgcag atcggcttct gccaatgtct cgcgctcttg cccggcgtgt
      721 cgcgatccgg cgccacgatt ctcggtgccg agctgctggg cgtggaacgc aaggccgcgg
      781 cggaattcac cttctacctg gcagtgccca ccatgctcgg cgcttcggtg ttcgagctgt
      841 acaaggaggg cgcgacgctc acgcgccacg atgaatcgct catcgcgatc ggctttgtcg
      901 tggccttcgt ggtcgcctat ggggtggtga ggacgttcat cgccttcatc gggcgctacg
      961 ggctgaaacc cttcggctgg tatcgaatcg catcggggct ggctctgttc gcgttcatct
     1021 gggcgcactg accggttcgc gcggcggcgc ttcgaactga ccttcgcgcc ggaagcgcca
     1081 gagataggtg gggatgatgg cttccaccgt ctccgcttcg atgccgagat cggcaagcgt
     1141 ggccgcgccc ggcgtaacca cattgtcctg cttgagcagc agcacctggt cgcgcgtgag
     1201 catcggcttc ggcaacaatc cgagaatgct tgcctgcatc atggcgaagg aaaagggcag
     1261 gggtatcaga aatcggcggc gtctggtctc ggcaagtacg agctgcatca attccctgaa
     1321 cgtatagacg cgcgggccgc cgagctcata gatgcgccct tgcgtcgctt gattcgccat
     1381 gcaggtcgca attgcccgcg cggtatcgcc cacatagacg ggttggaagc gtgtgtgacc
     1441 gccgccgatc aggggaagcg ccggcaacag gcgcgcaaga gctgcgaaac ggttgaagaa
     1501 atcgtcctcc ggtccgaaga cgatcgaggg acgcaggatc accgccgaag gaagggtttc
     1561 gcgcaggcgc atctcgcctt cggccttcgt gcgggcgtag agcgaaggcg attgtccgct
     1621 ggcgccgagc gcggagacat gaacgagcgc gttcacgccc tccgctctgg ccgactcagc
     1681 aatggttgcg ggcgaatccg catgcaggcg ctgaaatgtc tggcggcccg attcgtacag
     1741 aacgcccacg aggtttattg cagctgcggc gtccttcatg gcgcgcgcga cggaatcggc
     1801 atccgcgaca tctgttctga caagctggat ctggccgaca tgccccatcg gcaaaaggta
     1861 attgccgaga ttgggccggc gcacgccgac gcggatgcga tagccggcgc gggccaatgc
     1921 gcgcactacg tagcggccga ggaaacccga accgccgaaa acggtgacaa gcctggtcat
     1981 gaaaacccaa acctggaaat cgccggcgcc gtcttagcgc caacccggct tgttcggaag
     2041 gcgctactct tcgacgtatt gccgctcacc gctcgcgggc tggatataaa cagtgacagt
     2101 gcggcccgat tcggcgtcga caaagcgttc gctggtgcgc tcccagccgg gccccggccg
     2161 cgcctgggcg agaggcttgt agcggaagcg ctcatagacc acggccgcga acagaatgcc
     2221 gccccagatc agcagcatga agccgggcgg atcggcgccg agcaacaggc acactgcgcc
     2281 ggtgccgagc gcggccatgc acatgagcat cagtacgtta cgcagcattg gtcttcgcat
     2341 ccacgggctc caggatcacc gcggttccgt aagccacgat ctcgctcatc gtctggccga
     2401 tctccgcgga atcgaaacgc atcatgatca cgccgttcgc gcccataacg gttgcgttgc
     2461 gcaccagccg gtctacggcg tggcggcgcg cttcctcgag caattgcgtg tattcgtgaa
     2521 tctcgccgcc gacgatcgaa cgcagccccg ccatgatgtt gccgcccagt ccgcgcgaac
     2581 gcaccaccac gccgaagcac tggcccagcg tctgtttcgc ccggtatcct gccacgcctt
     2641 cggctgtcgc cacgatcatg tgcgcctccc tgctatccga gtcgagcgcc attatggcgg
     2701 gaaatggctt ccgatgccca gcgggtcgaa ccagacagcg gatgaaacct gtcgaaggca
     2761 cgtcgacccc tcacccgctc ctgaacggtt tcgacgcccg tatctgcatc cctctcccgc
     2821 aaggggcgag gggaatcccg agtcctgcat cgacgtcggg aaatcagcgc gagaaattcc
     2881 ctctccttgt gggagagtgg ctgagcgcga cgtccttgca accgatcagt cgcaggtgag
     2941 gggtgggcaa cacgctcgca tcctccatgg ttctttgctc gcgttgacag cacgggggcg
     3001 cgctcctaca catccccgcc ccacgggccc agatggcgga attggtagac gcactagttt
     3061 caggtactag cgccgcaagg cgtggaggtt cgagtcctct tctgggcacc agatttttta
     3121 cgctaattaa tcttcgtcac ggccgggaca acggccgggc catgacgaag atctagagaa
     3181 cgtccgcatc gctgcggagc gtgtgcggcg ttggcttgtc ttcaccgttg ggctatagcc
     3241 catgcaccgc agtataagtc ccatctggag agaacctcag gcgtgaagat caagttgcac
     3301 aaaggcgatc tgccggacgg gctcgatctg gcccccagcg tcgctatcga caccgaaact
     3361 ctcggcctca atcctttccg cgaccggctc tgtctcgtgc agctttcctc aggcgatgac
     3421 gtctgccatg cggtgcagtt cgcacccggt gaatatgccg cgccgaacct gaagcggatg
     3481 cttggcgatc cgaaggtgct gaagctcttc cacttcgggc gcttcgacat cggcatgctg
     3541 aaacggcatc ttggcgtcac ggccgcgccc gtctattgca ccaagatcgc gtcgcggctc
     3601 gcgcgcacct acaccgacaa acacgggctc aaggacatcg tgcgcgagtt gttgggcgtc
     3661 gagctttcca aggaacagca aagctcggac tggggtgcac ccaatctgag cgaaagacag
     3721 cttgcctatg ccgccaacga cgtggcgtat ctgcatcgac tcaaggtcgc gctggatgcc
     3781 atgctcgtcc gcgaaggccg cgcctctcat gcagaagcgt gctttgcctt ccttccggcg
     3841 cgcgtcgagc tggatctcgc cggctggcag gataccgacc ttttcgcgca ttgacgcgaa
     3901 gcggatggcg cgtcgactgg ttccccgcgc ttgaatgccg caggataaag ccgcataatc
     3961 ggatcggtcc ggcacgattt tcgcttaaga gccgtctccg tcgggggcgc attgaaaagg
     4021 ccgcggtgtt tctgggggag agcccgcggc tatattgttg acaggtgtcc tgcatgcatt
     4081 ccactgcccc gattgccgcg tcccgtgtgt tgacgcggaa agaaccttcg gggaatccgc
     4141 ccgacctcac agcagcgcgc cgcgtcctcg attgcgccac cgacgccatc gaaggattga
     4201 gcgcctccct cgatgggtct ttcagccgcg cggtcgatct catcctgggc atccggggcc
     4261 gcgtgatcgt cagcggaatg
//
LOCUS       DASZMC010000551         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3527672,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000551 DASZMC010000000
VERSION     DASZMC010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527672"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(441..1196)
                     /gene="aat"
                     /locus_tag="VG274_11195"
     CDS             complement(441..1196)
                     /gene="aat"
                     /locus_tag="VG274_11195"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWE07266.1"
                     /translation="MHGTPRFTAEMLLQAYAEGVFPMAQRRDGDELYFVSPEERGVIP
                     LDGFHVSHRLARTVRSDRFEVRIDSAFDDVIRACAAAAPGRAESWINAEIVTLYNALH
                     RRGNAHSVECWRDGRLAGGLYGVNLGAAFFGESMFSRERDASKVALVHLVARLRRGGF
                     ALLDAQFLTEHLARFGAIAVPRSTYLDCLDLAIHKTAEFPCGLYCPEPSGNSGTASFA
                     SASGDVGFTGAVEATTGATWPGWLVLQLITQTS"
     gene            complement(1207..>2220)
                     /locus_tag="VG274_11200"
     CDS             complement(1207..>2220)
                     /locus_tag="VG274_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWE07267.1"
                     /translation="LMGDKIAAKQAVAQLGIPTVPGSEGAVGSDADAAGIAAAIGYPV
                     LIKAAAGGGGRGMKVATRPEDLPLALTTARAEAKTAFADDSVYIEKYLDRPRHIEVQV
                     LADSFGKVLHLGERDCSLQRRHQKLWEEAPSPALNAAQREEIGHTVTSALSKLGYLGA
                     GTVEFLFEDGRFYFIEMNTRLQVEHPVTEAITGIDIVREQIRIAAASPVELEQSDIVI
                     AGNAIECRINAENPFSFQPSPGTITQFHAPGGLGVRFDSAMYTGYRIPPYYDSLAGKL
                     IVHGRNRNECLMRLRRALDEIVVGGIETTIPLFQKLVRETDILNGDYHIHWLEQYLAA
                     HGV"
BASE COUNT          421 a          706 c          699 g          394 t
ORIGIN      
        1 cgcgatctac ggaaactccg gaagcaaata atggcaatcg catcgacagg agttcgaatt
       61 gcctgcggca tcgctatggc cgcgcccgct ctgacgcagg cgcgcgcgga actggtgaat
      121 gttccgcaat cgcaaaaatc actgagcctt gccgatgccc tgccaggcaa ggacgggatc
      181 aagcgggcgc tagttctgcg ggggctcgac aagatcaccg gccgtccgac caacgtcttt
      241 gcgccggtcg gagtaccggt gaactttgct tcgctcacca tcacggcccg gtattgctac
      301 tcgaccccgc caagcgagcc gcccgagacg acggcgttcg tcgaaattca ggaccatcgg
      361 cccgatcagc cggcgcgcag cgtgttttcc ggctggatgc tggcgtccag tccatcgctg
      421 aacggcctgc aacatccgct ctatgacgtc tgggtaatca gctgcaaaac taaccagcct
      481 ggccaggtcg cccccgtggt ggcttctacc gctcccgtga agcccacgtc accggacgcc
      541 gaggcgaagg aagccgtgcc cgaattgccg gaaggttcgg gacagtagag gccgcacgga
      601 aactcggcgg tcttgtggat cgccaggtcg agacaatcca gataagtgct ccgcggaacg
      661 gcgatggcgc caaagcgcgc gagatgttcc gtaaggaatt gcgcatcgag gagcgcaaaa
      721 ccaccccgtc tcaagcgggc aacgagatga accagcgcaa ccttgctggc gtcgcgttcg
      781 cgcgagaaca tgctttcgcc gaagaaggcg gcgccgagat tcacgccata aaggccgccg
      841 gccagccttc cgtcgcgcca gcattcaacg ctgtgggcgt tcccgcgccg gtgcaacgca
      901 ttgtagaggg tgacgatttc cgcattgatc cagctttccg cccgtcccgg cgccgctgcc
      961 gcacaggccc ggatcacgtc atcgaacgcg ctgtcgatac gcacttcgaa acggtcgctg
     1021 cgcacggtgc gtgccaggcg gtgcgaaacg tgaaatccgt cgagtggaat gacgccgcgc
     1081 tcttcgggcg acacgaagta gagctcgtcg ccatcgcgcc gctgggccat cggaaatacg
     1141 ccttcggcat aagcctggag taacatctcg gcggtgaagc gcggcgttcc gtgcatcgca
     1201 tccacctcag acgccgtgag cggccaggta ctgctcgagc caatggatgt gatagtcgcc
     1261 gttcaggatg tcggtttccc ggaccagctt ctggaacagc ggaatcgttg tctcgattcc
     1321 gcccaccacg atctcgtcga gcgcgcgcct gagacgcatg aggcattcgt tccggttgcg
     1381 tccatgcacg atcagcttgc cggcgaggct gtcgtaatag ggcgggatcc ggtagcccgt
     1441 gtacatcgcc gagtcgaagc gcactccgag gccacccgga gcatgaaact gcgtgatggt
     1501 cccaggcgag ggctggaaag agaacggatt ttccgcattg atccggcatt caatcgcgtt
     1561 gcccgcaatc acgatgtcgc tttgctcaag ctcgaccgga ctcgcggcgg caattcgaat
     1621 ctgctcgcgc acgatgtcga tgcccgtgat cgcctcggtg acaggatgct cgacctggag
     1681 ccgcgtgttc atttcgatga aataaaaccg cccgtcctcg aacagaaact cgaccgtacc
     1741 cgcgcccaga tagccgagct tcgacagcgc gctcgtgacg gtgtgcccga tctcttcgcg
     1801 ctgggcggcg ttcagggcgg gggagggcgc ttcctcccag agtttctgat gtcggcgctg
     1861 caacgagcaa tcacgctcgc caaggtgaag caccttgcca aaggaatcgg cgagcacctg
     1921 cacttcgatg tgccgcggac ggtcgagata tttctcgatg tagacggagt cgtcggcaaa
     1981 ggcggtcttc gcctcggcac gcgccgtggt aagggcgagc ggcaggtcct cgggccgcgt
     2041 ggcgaccttc atcccgcggc cgccgccgcc cgcggcggcc tttataagga cgggatagcc
     2101 gattgcggcc gcgatcccgg cggcgtcggc atcgcttccg acggcgccct cggagccggg
     2161 aacggtggga attccgagct gcgccacggc ctgtttggcg gcaatcttgt cgcccatcag
//
LOCUS       DASZMC010000552         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_228273,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000552 DASZMC010000000
VERSION     DASZMC010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228273"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2419)
                     /locus_tag="VG274_11205"
     CDS             complement(<1..2419)
                     /locus_tag="VG274_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWE07268.1"
                     /translation="MLSQARQRKLEGIDVVIAVVETHGRVETDLLTKSIEKIPKKRVA
                     YKGRVLAEMDLDAVLQRRPKLALVDELAHTNIDGSRHPKRYMDVEELLAAGIDVYTTL
                     NVQHLESLNDVVAKITRVRVGETVPDSVLDRADDVELVDLTPEDLLQRLKEGKVYLPE
                     AAEWAAQNYFVPGNLTALRELALRRTAQRVDDQMVSYMRAHAIQGPWEASERVLVCVN
                     ERPGAGSLVRYARRLAGRLRASWTAIHVETPGTRDMSETERDRVADALRVAERLGGQG
                     VSVPAASVADGVIDYAHANNFTHIVTSTAQRSWWSELIRGSAAHEIIRRAGDVSVHVV
                     PEQLTRAMKGATHRGPQSDKAGPSVWDSKAFAGSFGMVIVALLIGLVFQKFGITNVAL
                     VFLTAVLASAITYGLWPSLFACLVAILAYNFFFLPPLYTFTIAATENIVALFFFTVVA
                     LIASYLSARMRAQALSARERAATTENLYLFSRKLAVVVTLDDLLWATAFQFAQMLKLR
                     VVILLPQDTSIAVRAGYPPEDTLDQADLAAAKWVWQHGSAAGRGADTLPGAKRLFLPM
                     RTGRGTVGIVGLDSDRLGPPLTPDQKRLFDALSDQAALAIERINLSEDIDRARLAAET
                     ERLRSALLTSISHDLRTPLASILGSASSLNSQRGNLDEGAREELIGTIQEEAERLNRF
                     IANLLDMTRLESGAIEPRLDFVDLGDVVGSAIRRATGVLACHQVQIDLAPDLPMLRLD
                     PVLFEQVLFNLLDNAAKYAPPDTKIGLHARQDGDLVRLEIEDEGDGIPANDLDRIFDK
                     FYRVQAMD"
BASE COUNT          449 a          770 c          819 g          464 t
ORIGIN      
        1 ggtccatggc ctgcacgcga tagaacttgt cgaagatccg atcgaggtcg ttggcgggaa
       61 tgccgtcgcc ctcatcttct atttccaggc gaacgagatc gccgtcctgc cgggcatgca
      121 gcccgatttt cgtatctggt ggcgcatatt ttgcggcgtt gtcgagcaga ttgaacagca
      181 cctgttcgaa gaggacggga tcgagcctga gcatcggcag atcgggcgcg aggtcgatct
      241 ggacttgatg gcaagcgagg acgccggtag cgcggcggat tgcgctgccc accacgtctc
      301 ccagatccac gaaatcgagc cgcggctcga tggctccgga ttcgagccgc gtcatatcca
      361 gaaggttcgc gatgaagcga ttcagccgct cggcttcctc ctggatcgtt cctatcaatt
      421 cctcccgggc gccttcgtcc agatttcccc gctggctgtt gaggctgctg gccgaaccga
      481 ggatggaggc caacggcgtt ctgagatcat gcgagatcga ggtgagaagt gcggaacgca
      541 ggcgttcagt ctctgcggcg aggcgcgcgc gatcgatatc ctccgacagg ttgatgcgct
      601 cgatcgcaag cgccgcctga tccgacagcg catcgaaaag cctcttctgg tccggcgtga
      661 gcggggggcc gagccggtcg ctgtcgagac ctacaatgcc gaccgtgcca cgcccggtcc
      721 gcatcggaag aaagaggcgt ttggcgccgg gcagcgtgtc ggcgccgcgc ccggcagccg
      781 aaccatgctg ccaaacccat ttcgcggccg ccagatcggc ctggtcgagc gtgtcctccg
      841 gcggatagcc ggcgcgtacg gcgatggagg tgtcctgggg aagcaggatc acaacgcgga
      901 gcttgagcat ctgcgcgaac tgaaatgcgg tagcccaaag gagatcgtcg agggtgacga
      961 cgaccgcgag cttgcggctg aacagataga gattctccgt tgttgcggca cgctcgcgcg
     1021 ccgacagcgc ttgggcgcgc atgcgcgcgg aaagatagct tgcgatcagc gcgacgacgg
     1081 tgaagaagaa cagcgcgacg atgttctcgg tcgcggcaat cgtgaaggtg tagagcggcg
     1141 ggaggaagaa gaaattgtag gcgagaatcg cgacgagaca ggcgaagagc gagggccaca
     1201 gtccgtaggt gatcgcgctc gccagcactg cggtgagaaa tacgagtgcg acgttggtga
     1261 tcccgaattt ctgaaagacg aggccgatca ggagggcgac gatcaccatg ccgaagcttc
     1321 cggcaaaggc tttcgagtcc cagacggagg gtccggcctt gtcactctgc ggtccgcgat
     1381 gcgtcgcacc tttcatcgcg cgcgtcagtt gttcgggaac gacatgcacg ctgacatcgc
     1441 ccgcgcggcg aatgatctcg tgcgccgccg agccgcgaat gagctcagac caccagctgc
     1501 gttgcgcggt cgaggtgacg atgtgggtaa agttgttggc gtgcgcgtag tcgatcaccc
     1561 cgtcggcgac gctcgcggcc ggcacgctga cgccctgtcc gccgagccgc tcggcgaccc
     1621 gaagcgcatc cgcgacgcga tcgcgctccg tttcgctcat gtccctcgta cctggagtct
     1681 ccacgtggat tgcagtccag gaggcacgga gcctgcccgc cagccggcgc gcatagcgca
     1741 ccagagaccc cgcgcccgga cgttcgttca cacagacgag aacgcgttcg ctcgcttccc
     1801 acggcccctg gattgcgtgc gcgcgcatgt agctgaccat ctggtcgtcg acgcgttgtg
     1861 cggttcggcg cagcgcaagc tcgcgcagcg cggtcaggtt gccgggtacg aagtagttct
     1921 gcgcggccca ctcggccgct tcgggaagat agacctttcc ctccttgagg cgctgaagca
     1981 ggtcttcggg cgtcagatcg actagctcga catcgtcggc gcgatccaga acggaatccg
     2041 gcaccgtctc gcccacgcgc acgcgcgtga tcttcgccac gacatcgttc aggctctcga
     2101 gatgctgcac gttcagcgtg gtatagacgt cgattccagc ggcaagcagt tcttccacgt
     2161 ccatgtagcg cttgggatgg cggctgccgt cgatattcgt gtgcgcgagt tcgtccacca
     2221 gcgcgagctt cggccggcgc tggagaacgg cgtccagatc catttcggcc aggacccggc
     2281 ccttataggc gacgcgcttc ttgggaattt tttcgatgct cttggtcaac aggtcggttt
     2341 cgacgcggcc gtgcgtttcc acaactgcga tgacaacgtc gatgccttcg agcttgcgct
     2401 gtcgcgcctg actcagcatc tcatgggtct tgccgactcc cggcgccgcg ccaagaaaga
     2461 ttttcaaccg gccgcggcct tccttcgccg cctggtcgag aa
//
LOCUS       DASZMC010000553         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_795079,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000553 DASZMC010000000
VERSION     DASZMC010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795079"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VG274_11210"
     CDS             complement(<1..885)
                     /locus_tag="VG274_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE07269.1"
                     /translation="MIALEVRNLSKFYPIGGSQWSRFRHVLAAGRVPRDRGIRALYDV
                     SFHVERGEAFGVVGANGSGKSTLLQIVAGILQPTSGSVTANGRLSALLELGSGFSPEF
                     TGRENVYLNASILGLAKEEIDARFAAIEAFASIGDFIDRPIRTYSTGMVLRLAFAVVA
                     HVDPEILIVDEALAVGDIAFRQRCMKRIHALRGAGVTILFVSHETGDVKALCERCVWL
                     DKGEVRALGDTDSVIADYLSATLVRERAHNLHAPAAHVNAAAEAPGDIEGRRRYGSGA
                     ARFISAELLGERDVVLRAK"
     gene            complement(882..1694)
                     /locus_tag="VG274_11215"
     CDS             complement(882..1694)
                     /locus_tag="VG274_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWE07270.1"
                     /translation="MNTTDFIERARAAGLEDADRYFWYHTVDLGDGLITPGLYDYRDT
                     IAAFQFPDDMRGMTVLDVGSATGYFAFEFERRGARVISVELPSLRDLDRFPGQDVESS
                     LRKIEAMAFADRGYSERDLYWYLLEGPFRFCRERLRSRVERCYSTVYDLTLERTGVRE
                     GFDLVFVGDVLIHTLYPLKALASLAALCKGMLVFAGVLPDGPQEPPAMAYIGGDDLAG
                     DEVAWWHPNLSCLVQILRKLGFASVEQVGFHRGLLQPAGFPFERVILRAVKP"
     gene            complement(1691..2455)
                     /locus_tag="VG274_11220"
     CDS             complement(1691..2455)
                     /locus_tag="VG274_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWE07271.1"
                     /translation="MNAEQIRKGIEQFGPWFYSFDFGEGLRTTPAIPPEVAGIHDTRL
                     AMLDAAVSRHFGGRTSVLNCLDIGCHQGFYSIAMARRGWNVTGVDARDESLFAARFAS
                     SAAGFPQIAFHRGLVETLARDENRAYDLTLFLGVLYHVEDPMLCLRQVAAVTKEMCVI
                     ETQVVDEVEGYAEWGARAWTRPYQGILALIDESGEFQAGNRETGVTPMATCPSPKALL
                     FMCRQAGFARAEILSPPPGAYEQHARGKRVVCAAWK"
     gene            complement(2452..3219)
                     /locus_tag="VG274_11225"
     CDS             complement(2452..3219)
                     /locus_tag="VG274_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HWE07272.1"
                     /translation="MNYTINNDEQLLVLEEGGLYREIPLYSREAYETLSTQWLRVGWA
                     LGSYYTFSWAGQPLLQLPEDVLRLQEVMFDLKPAVIVETGVCGGGSLLLHATVCEAMG
                     AGRVIGIDRHIPESTRAGVESHRLSHRISMIEGDSAAPSTIEKVREIVGTSAPVMVIL
                     DSDHSLAHVSDELQSYAPLVTPGSCIVAEDGVMRDLTDVPGGSATWNFDNPATAARAF
                     LAAHPEFEMREPAWKYNRGELRRNVTYWPDGWLWRTR"
     gene            complement(3227..>5006)
                     /locus_tag="VG274_11230"
     CDS             complement(3227..>5006)
                     /locus_tag="VG274_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWE07273.1"
                     /translation="HSETDPAAQVKIWSLREAALGLSMASREDAKSISFVEDTAVAPE
                     KLSAFIGEFLKIVAAHNTTAGIYAHASVGCLHVRPVVNMKTAEGVAKFESIANAVADL
                     VLEYGGALSGEHGDGLVRSPFMERMFGPVLYSAFREIKRTFDPEGLFNPGKIVDAPSM
                     TANLRYGPGYKTTQPFTFFDYSEYGGMAGAVEMCSGVGACRKKLSGTMCPSYMVTREE
                     GASTRGRANVLRMAMSGRLDEAGLGDDGVYNVLDLCLECRACKSECPVGVDVARFKSE
                     FLADYYTRHGTPARARALGNVHKTAVWGSRFATIANAVAARPLVRRLNEKFLDIDARR
                     TLPAWRSETFAHWSRRHKRAPGTPVTLFNDTFTNHYDPEIGSAAVEVLERGDCSVGVV
                     RPGCCGRPLISQGLLSEARAHAAKLIDALHPIASRGESILFLEPSCLSAVKDDAIGLL
                     RGERQSKARTVARAARLFDEFASELALSLRPVAGRILLHGHCHQKSLGLLPATMALLA
                     KIPEAKVVDLDAGCCGMAGSFGYTRGHYDVSKAIAERKLLPAVRQMQPGDILVAPGTS
                     CRHQVLDFANANAVHPAVLIRSLLTS"
BASE COUNT         1001 a         1496 c         1629 g          880 t
ORIGIN      
        1 ctttgcgcga aggacgacat cgcgctcgcc gaggagctcg gcacttatga agcgggcggc
       61 gccggagccg taccggcggc gtccttcgat atcgccgggc gcctccgccg ccgcgttcac
      121 gtgcgctgcg ggagcgtgca ggttgtgagc gcgctcccgc acaagagtgg cgctgaggta
      181 atccgcgatt acggaatcgg tatcgccgag tgcacgtact tcgcctttat ccagccatac
      241 gcagcgctcg caaagcgcct taacatcgcc ggtctcatgc gacacgaaca ggatggtcac
      301 gccggcgccg cggagcgcat gaatgcgctt catgcagcgt tgacgaaagg cgatgtcgcc
      361 gacggcgagc gcctcgtcca cgatcaggat ttccggatcg acatgggcga ccaccgcgaa
      421 ggcgagacgc agcaccatgc cggtggaata cgttcggatt ggccgatcga tgaagtcccc
      481 gatcgaggcg aaggcttcga tcgcggcaaa gcgcgcgtcg atttcttctt ttgcgagtcc
      541 gaggatggag gcgttgaggt agacgttctc gcggccggta aattcgggtg aaaagcccga
      601 gccgagctcc aggagagcgg agagacgtcc gttggcggtc acggaacccg aggttggctg
      661 aaggattccg gcgacgatct ggaggagtgt gctcttgccg gagccgttgg cgccgaccac
      721 accgaaagcc tcaccccgtt cgacgtgaaa gctgacatca tacagcgctc gaatgccgcg
      781 gtcgcgggga acgcggccgg cggcgagcac atgccggaag cgcgaccact gcgatcctcc
      841 gatggggtag aacttcgaga gattgcgaac ttccagcgcg atcacggttt gacagcgcgc
      901 aggataacgc gctcgaaagg aaatccggcc ggttgcagca gtccccggtg aaaaccgacc
      961 tgctcgacgg acgcgaagcc cagcttgcgc aagatctgca ccagacagga aagattggga
     1021 tgccaccagg cgacctcgtc ccccgcgaga tcgtcgccgc cgatgtaggc catcgcggga
     1081 ggctcctgcg ggccatccgg aaggacgccc gcaaatacga gcatgccctt gcaaagtgca
     1141 gccagtgacg cgagtgcttt cagaggatac agcgtgtgga tcagaacatc gcccacaaaa
     1201 acgaggtcga aaccttcgcg aacgcccgtg cgttcgaggg tgaggtcgta aacggtcgag
     1261 tagcagcgtt ccacgcgcga acgcaggcgt tcacggcaaa agcggaaggg gccttccagc
     1321 agataccagt acagatcgcg ctcagagtag ccgcggtccg cgaaggccat ggcttcgatt
     1381 ttgcgcaatg agctttccac gtcctggccg gggaagcggt cgagatcgcg aagcgaggga
     1441 agttcaacgg aaatcacgcg ggcgccgcgg cgctcgaatt cgaacgcgaa gtatccggtg
     1501 gcggagccga cgtcgagcac ggtcatgccg cgcatgtcgt caggaaactg gaaggccgcg
     1561 atggtgtcgc ggtaatcgta gagacctggc gtaatcaggc catcgccgag atcgaccgtg
     1621 tggtaccaga agtatcggtc ggcgtcttcg agaccggcgg cgcgggcgcg ctcgatgaaa
     1681 tccgtcgtgt tcatttccag gccgcgcaga ccacgcgttt gccccgcgca tgctgttcgt
     1741 aagcgccggg gggcggcgaa aggatttcgg cgcgagcgaa accggcctgg cggcacatga
     1801 atagcagcgc cttcggcgag ggacaggtcg ccatgggagt gacgccggtc tcgcggttac
     1861 cggcctggaa ctcgccggat tcatcaatca gggcgagaat gccctgatac gggcgggtcc
     1921 acgcgcgcgc accccactcc gcgtaccctt ctacttcatc gaccacctga gtttcgatca
     1981 cgcacatctc tttggttacg gcggcgacct ggcgcagaca cagcatcggg tcttcgacgt
     2041 gatagagcac gcccagaaac aacgtgagat cgtacgcgcg gttttcgtcg cgggcgagtg
     2101 tttccaccag gccgcgatga aatgcgattt gcgggaagcc ggcggcggag gacgcaaaac
     2161 gcgccgcgaa cagactctca tcgcgggcgt cgacgccggt cacgttccaa ccgcgccgcg
     2221 ccatggcgat cgaataaaaa ccctggtggc agccgatatc gaggcaattg agaacgctcg
     2281 tgcgcccgcc gaagtggcgg gatacggcgg cgtcaagcat cgccaggcgt gtgtcgtgaa
     2341 tgcccgcgac ttcgggcgga atggcggggg tggtgcgcag gccttcgccg aaatcgaagg
     2401 aatagaacca ggggccgaac tgttcgattc ccttgcgaat ctgctccgcg ttcaacgggt
     2461 tctccagagc cagccatcgg gccagtaggt cacgttgcgg cgcaactcgc cgcgattgta
     2521 tttccatgcg ggctcgcgca tctcgaattc gggatgggct gcgaggaatg cgcgcgcggc
     2581 ggtagcgggg ttgtcgaaat tccaggttgc gctgccgccg ggcacatcgg tcaagtcgcg
     2641 catcacgccg tcttcggcaa cgatgcacga gccgggcgta acgagaggag cgtaggactg
     2701 aagttcatcc gagacgtggg cgagcgagtg atccgaatcg agaatcacca tcacgggcgc
     2761 gcttgttccc acgatttcgc ggactttttc gatcgtcgag ggcgcggccg aatcgccttc
     2821 gatcatcgag atgcgatgcg acaaccgatg actctcgacg cccgctcggg tggattccgg
     2881 aatgtgccgg tcgattccga ttacgcgccc ggcgcccatg gcttcgcaga cggtcgcgtg
     2941 gaggaggagc gaaccgccac cgcaaacgcc ggtctcgacg atcacggcgg gtttgagatc
     3001 gaacatgact tcctgcaatc ggagcacgtc ttcgggaagc tgtagcagcg gttgcccggc
     3061 ccaactgaac gtgtaataag agccgagcgc ccaacccacg cggagccact gggtgctgag
     3121 ggtttcgtaa gcctcccggc tgtaaagcgg gatctcccga tacaggccgc cttcctcaag
     3181 cacgagaagt tgctcgtcgt tattaatcgt gtagttcaat tttcaattat gacgtgagga
     3241 ggcttcgaat caggacggcc ggatgcacag cgttggcgtt tgcgaagtcg agcacctggt
     3301 gacggcacga cgtgcccggc gcgacaagga tatccccggg ttgcatctgg cgcacggcag
     3361 gcagaagctt ccgctcggcg attgctttcg agacatcgta gtggcctcgg gtgtaaccga
     3421 aagagcccgc cattccgcag cagccggcat caagatcgac cactttggct tcgggaattt
     3481 ttgcgagcag ggccatggtg gcggggagaa ggccgagcga tttctggtga caatgcccgt
     3541 gcagcagaat cctgcccgca acggggcgca gggacaacgc tagttcggaa gcaaattcgt
     3601 cgaagaggcg cgccgcgcgc gctacggtgc gtgctttact ctgccgttcg ccgcgcagca
     3661 atccgattgc gtcgtccttg acggccgaga gacagcttgg ttcgaggaac agaatggact
     3721 cgccgcgcga tgcaatcggg tgcaaggcgt cgatcagctt tgcggcgtgc gcgcgcgctt
     3781 cggagagcag tccttgcgag atcaaaggac gcccgcagca accgggacgc accacgccga
     3841 cgctgcaatc gccccgttcg agcacttcga ccgcggcgct gccaatttcg ggatcgtagt
     3901 gattggtgaa cgtatcgttg aaaagagtga cgggcgttcc gggcgcgcgt ttgtgacgtc
     3961 ggctccaatg cgcgaaagtt tccgaacgcc acgccggaag ggttcgccgc gcatcgatat
     4021 ccaggaattt ctcattgagc cggcgcacca ggggtctcgc cgcgacggca ttcgcgatag
     4081 tcgcgaagcg gcttccccac accgcggtct tatggacgtt gccgagcgca cgggcgcgtg
     4141 cgggcgtgcc gtgccgagtg taatagtccg ccagaaattc gcttttgaag cgcgccacat
     4201 cgacgcccac ggggcattcc gatttgcagg cgcggcattc gagacacaag tcgagaacgt
     4261 tgtaaacgcc gtcatcgccg aggcccgctt cgtccagacg cccgctcatc gccattcgca
     4321 gcacgttggc gcgtccacgc gtggaggctc cctcctcgcg cgtcaccata tacgagggac
     4381 acatggttcc ggaaagcttc ttgcggcatg cgccgacgcc gctgcacatt tcaaccgcgc
     4441 ccgccattcc gccatactcc gaataatcga agaacgtgaa aggctgggtg gttttgtagc
     4501 cggggccgta acgcaggttc gcggtcatcg agggagcatc cacgatcttg ccgggattga
     4561 agagaccttc gggatcgaac gtcctcttga tctcgcggaa cgcggaatac agcacgggac
     4621 cgaacatccg ctccataaac ggactgcgaa cgagcccgtc gccatgctca ccggaaagcg
     4681 cgcctccgta ttcgagcacg agatcggcaa cggcgttggc gatggattcg aatttggcga
     4741 cgccctccgc tgtcttcatg tttacgaccg ggcgcacgtg caggcaaccg acggacgcgt
     4801 gcgcataaat gcccgccgtc gtgttgtggg cagcgacgat tttgaggaat tcgccaatga
     4861 aggcgctcag cttttccggg gcaaccgctg tatcttcgac aaatgaaatg gatttcgcgt
     4921 cttcgcgcga cgccatggag agacccagcg ccgcttcgcg caggctccag atcttcacct
     4981 gcgcggcggg atcggtttcc gaatga
//
LOCUS       DASZMC010000554         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2146100,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000554 DASZMC010000000
VERSION     DASZMC010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146100"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1309
                     /locus_tag="VG274_11235"
     CDS             191..1309
                     /locus_tag="VG274_11235"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HWE07274.1"
                     /translation="MTAAPAKPLGMTLLERIGNTPLLRLESLTAHLQGVQVLGKAEWA
                     NPGGSVKDRAASAIVGDAQKKGLLTAGNASSGMPRASSEMPRALSEMPRAVSGMPRAL
                     SGMPRALLDATSGNTGIAYAMLGAAQGFAVTLCMPSNVSPERRRILDAYGATIVWTDP
                     SEGSDGAIRMARKLASEQPDRYFYADQYGNDNNWRAHYYGTANEIWEQTEGRVTHFVA
                     GLGTSGTFMGTTRRLRELNPTIQCISMQPDSPFNGLEGLKHMATAIVPPIYDANLADR
                     NIDMPTERAYAMAKRLGRTQGLLVGVSAAAAVAASLQVAEEEARAGREAVIVTILCDS
                     ADKYLTERFWSEPSENESSGDAAAADAVDSATYEGQRA"
     gene            1306..1740
                     /locus_tag="VG274_11240"
     CDS             1306..1740
                     /locus_tag="VG274_11240"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWE07275.1"
                     /translation="MSLILPRALYDEMRAHGEETYPNECCGIMLGTADAGAIRVAELM
                     RAGNTRTDSAHNRYNIAPQELIAAQREGRRKGLDIVGFYHSHPDHPAQWSRTDFAEAH
                     WFGCAYVITGVDRGKARTTNSFLLDGTGEDDKAFCAQAIAVE"
     gene            1769..2071
                     /locus_tag="VG274_11245"
     CDS             1769..2071
                     /locus_tag="VG274_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWE07276.1"
                     /translation="MTINIPTPLRSYTDRQDAVSVHGVTVAEGLEQLVKQFPAMRQHL
                     FTTEGKLRSFVNVYVNDEDVRYLPEKENTGVADADELTIIPSIAGGCPCRAAGCCR"
BASE COUNT          429 a          695 c          774 g          380 t
ORIGIN      
        1 ggttgtttct tgagctgttt catgatcctg ggcaatgcag ctactctggt ttcctgctcg
       61 cggacgggtt tccgccggta gactcagctt agactgtcgc acatcggtgc ggcaggttca
      121 aacccggcgc ggcctctggc tgaagccgcg ccgcatggaa gaggggtacc cgcagttggg
      181 cgtggagacc atgaccgccg cacccgcaaa gccgcttggc atgacgctgc tggagcgcat
      241 cggcaacacg cccctgctgc ggctggagag tctgaccgcg cacctgcaag gcgtgcaggt
      301 cctggggaag gccgaatggg ccaacccggg cgggagcgtc aaggaccggg cggcctcggc
      361 catcgtgggc gatgcgcaga agaagggcct gctgacggcg ggcaatgcgt cttccgggat
      421 gccgcgggcg tcttccgaaa tgccgcgggc gctttccgaa atgccgcggg cggtttccgg
      481 gatgccgcgg gcgctttccg gaatgccgcg ggcgctgctg gacgcgacct ccggcaacac
      541 cggcattgcg tatgcgatgc tgggcgcggc gcagggcttt gccgtgacgc tgtgcatgcc
      601 gtccaatgtg tcgccggagc gcaggcggat cctcgatgcg tatggcgcga cgatcgtgtg
      661 gaccgatccg tccgaggggt ccgacggcgc gattcgcatg gcgcgtaagc tcgcctccga
      721 gcagccggac aggtacttct atgccgacca gtacggcaac gacaacaact ggcgcgcgca
      781 ctactacgga accgccaacg agatatggga gcagacggag ggccgcgtga cccattttgt
      841 ggccgggctg ggcacgagcg ggacgtttat gggaacgacg cggcggctgc gcgagctgaa
      901 cccgaccatc cagtgcatct cgatgcagcc ggattcgccc ttcaacggcc tggaaggcct
      961 gaagcacatg gccacggcga ttgtgccgcc gatctacgat gcgaacctcg ccgaccgcaa
     1021 catcgatatg ccgaccgaac gcgcctacgc catggccaag cggctggggc gcacccaggg
     1081 tctgctggtc ggcgtgagtg cggcggccgc ggtcgcggca tcgctgcagg tggccgagga
     1141 ggaggcgcgc gccgggcgcg aggcggtgat tgtcaccatc ctttgcgact ccgcggacaa
     1201 gtatctgacc gagcggttct ggagcgagcc gagcgaaaac gagtccagcg gggatgcggc
     1261 ggccgccgat gcggtggaca gcgccaccta cgagggtcag cgcgcatgag cctgattctt
     1321 ccgcgcgctc tctacgacga gatgcgcgct cacggtgagg agacctatcc caacgagtgc
     1381 tgcgggatca tgctgggcac ggcagatgcc ggagcgatcc gcgtggcgga gctgatgcgc
     1441 gcgggcaaca cgcgcaccga ttcggcccac aaccgctaca acatcgcacc gcaggagctg
     1501 atcgccgcgc agcgcgaggg gcgcaggaag ggcctggaca tcgtcggctt ctatcactct
     1561 cacccggacc atcccgcgca gtggtcccga accgatttcg ccgaggcgca ctggtttggc
     1621 tgcgcctacg tcatcaccgg cgtggaccgc gggaaggccc ggacgacgaa ctcgtttctg
     1681 ctggatggaa cgggcgagga cgataaagcg ttctgcgcgc aggcgattgc agtcgaataa
     1741 agccggctga tgggcgataa tagagacaat gacgattaat atccctacac cgctgcgctc
     1801 gtacaccgac aggcaggatg ctgtctcggt gcacggcgtt actgtggcgg agggactgga
     1861 gcaactggtc aagcagttcc cggccatgcg ccagcatctc ttcaccaccg agggcaagct
     1921 gcgttcgttc gtcaatgtat atgtgaacga cgaagacgtg cgctacctgc ctgagaagga
     1981 aaacaccggc gttgccgacg ccgatgaact gacgatcatc ccctcgatcg ccggcggctg
     2041 tccgtgccgc gccgcaggtt gttgtcgcta gacgcgagaa ccctggcaag cccttttcta
     2101 caatcgaatc gataccgccc gatgggacat ctgttccaat gggagatgcg tcgagttccc
     2161 gcaggcagca aagcagatcc ttcgctccgc tcaggatgac aatttcaggg attcaagatg
     2221 acgaatccgt agcggcgctg aagggttgag gacaacacca gatgattgcc gttgaaga
//
LOCUS       DASZMC010000555         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4083957,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000555 DASZMC010000000
VERSION     DASZMC010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4083957"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VG274_11250"
     CDS             <1..363
                     /locus_tag="VG274_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWE07277.1"
                     /translation="QPAPPRQVKAWLYRTVRNEAITQWRSSRSRGRRERQAALPESMF
                     EYDATAGGDVEALTTAIAKLPPTQREILILRMWGELTLAEISELIGAPVSTVFQNYRT
                     ALGTMRKWMEEPCQKKTH"
     gene            342..821
                     /locus_tag="VG274_11255"
     CDS             342..821
                     /locus_tag="VG274_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07278.1"
                     /translation="MPEEDALNAAERETVAAIARFKPATIRIDLFRIRSEAQRRRAKR
                     QVWLWRGIAAALAAGLALSWIWRPGSAVREHVIYVRSPAPAITPEASSPTFAATRIEP
                     RVPALASDDYLAVRDRVLAFGIDALRPHPPAVRHCNPPPIPDASPWHAMDILGGGSL"
     gene            836..>1934
                     /locus_tag="VG274_11260"
     CDS             836..>1934
                     /locus_tag="VG274_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07279.1"
                     /translation="MLTIVAIVVQIGRAQAQATTQPKPIEVRIHPMALAEPALKDRLL
                     PPYSEQTPGNAAPLYLTAFSLVQSNSDSWDQKRSDEFDELMDASIDDLDAEKARRFLV
                     PNVFELLDIAGRRSRCEWDTSMREQGYRALLPYLNHARTSANLLSIQIKLDIKQGDFD
                     HAIQMLRSGFAMANHLETEPILVQCLVAAGIREVMLSDVRALIQRPDAPNLYWPLADL
                     YGSRDLWPRIMESEREALFDTIPQLRHPEDLTAEQARKVIEEVNAYTDQLRGSDPQDA
                     LGRDVATLLKIIRAYPLARDALIRRGTPAAVVDSMPANAVALAWDVDGFQRRADAMER
                     WTCLPPWQALAGLRREAIVHREDDDLNPLKQL"
BASE COUNT          368 a          622 c          607 g          337 t
ORIGIN      
        1 cagcccgccc cgccgcgtca ggtgaaagcg tggctttacc gcaccgttcg gaacgaggcg
       61 atcacgcaat ggcgttcgtc gcgcagccgt ggacgacgcg agcgccaagc cgctctgccc
      121 gaatcgatgt tcgagtatga cgcgacggcc gggggagacg tggaagcgct gacgacggca
      181 atcgcgaaat tgccgccgac tcagcgggaa atccttatcc tacggatgtg gggcgagttg
      241 acgctcgcgg aaatctcgga actgatcggg gcgccagtct cgaccgtgtt tcagaattac
      301 aggacggcac tgggaacgat gagaaagtgg atggaagaac catgccagaa gaagacgcat
      361 tgaacgccgc agagcgcgag actgtggcgg ccatcgcccg attcaagccc gccacgattc
      421 ggatcgatct ttttcggatc cgatcggaag cgcagcggcg ccgtgcaaag cgccaggtat
      481 ggctatggcg cggcatcgcg gcggcgctcg cggcgggtct ggctctctca tggatctggc
      541 ggccgggcag cgctgtccgc gagcatgtga tctacgtgcg ttcgccggcc cccgcaatta
      601 ctccggaggc gtcatcgccc acattcgccg cgaccaggat cgagccgcgg gtgccagcgc
      661 tggccagcga tgattatctc gcggtccgcg accgcgtact cgcctttggc attgacgcct
      721 tgcggccgca tccgcccgca gtgcggcact gcaacccacc gccgatcccg gacgcgtccc
      781 cctggcacgc catggatatc ctgggcggag gttcactatg agatcccgaa tttgcgtgct
      841 gacgatcgtg gcaatcgtcg tccagatcgg ccgcgcgcaa gcgcaggcaa ccacgcaacc
      901 caagccgatc gaggtgcgca tccatcccat ggcgttggcc gagcctgccc tcaaggaccg
      961 gctgctcccg ccgtactctg agcaaacgcc gggcaacgcg gcaccgctct atctcaccgc
     1021 cttttcattg gtgcagtcga actccgattc atgggaccaa aagcggtccg acgagttcga
     1081 tgagctgatg gacgcatcca ttgatgacct ggacgcggaa aaggctaggc gcttcctggt
     1141 gccgaacgta tttgagctgc tcgacattgc cggccgtcga tcgcgctgcg agtgggacac
     1201 gtcgatgagg gagcaaggct accgcgcgct gctgccttac ctgaaccacg cccgcacgtc
     1261 ggccaacttg ctctcaattc agatcaagct cgacatcaag cagggcgatt ttgatcacgc
     1321 cattcagatg ttgcgatccg gcttcgcgat ggccaaccac ctggaaaccg agccgatcct
     1381 ggttcaatgt ctggtcgccg cgggcattcg cgaagttatg ttgagcgacg ttcgagcgct
     1441 gatccagcgt cccgacgccc cgaacctgta ctggcccctg gccgaccttt acggctcgcg
     1501 cgatttatgg ccgcgcatca tggaatccga gcgggaggcg ttgtttgaca cgattccaca
     1561 acttcgccat ccagaggacc tgaccgccga acaggcccgc aaggtgatcg aagaggtcaa
     1621 tgcatacacg gatcaattac gagggtcgga cccccaggac gccctcgggc gagacgtcgc
     1681 gactctcctg aaaatcattc gcgcctatcc gctggcccgg gacgctctga tccggcgcgg
     1741 gacgccggcc gcagtggttg acagcatgcc ggccaacgcg gtcgcgctgg cgtgggatgt
     1801 ggacgggttt cagcggcgcg ccgatgcaat ggagcgatgg acctgcctcc cgccctggca
     1861 ggctttggcc gggctcaggc gggaggccat cgtccaccgc gaagacgacg atctcaaccc
     1921 tctcaagcaa ctgg
//
LOCUS       DASZMC010000556         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_796251,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000556 DASZMC010000000
VERSION     DASZMC010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796251"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VG274_11265"
     CDS             complement(<1..146)
                     /locus_tag="VG274_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HWE07280.1"
                     /translation="MAEAQKHLFTSESVTEGHPDKIADQISDAVLDAVLEQDPYGRVA
                     CEVLV"
     gene            complement(311..1150)
                     /gene="rsmI"
                     /locus_tag="VG274_11270"
     CDS             complement(311..1150)
                     /gene="rsmI"
                     /locus_tag="VG274_11270"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWE07281.1"
                     /translation="MPGRLYIVSTPIGNLEDITYRAVRILGEADVIACEDTRTTRRLL
                     DHYGISTGAISYHEHNEQERAKELIARLESGVSIALVSDAGTPLLSDPGYRIVRAAVE
                     SGITVEALPGPSALLTALVASGLPTDQFHFAGFFPPKESQRIRVLESLRDEPATLIFY
                     EAPHRILETLADIESVLRDREIAVARELTKIHEELLRGTAASIRQTLSARDAIRGEFV
                     ILIAKAMKPAGDETPVDQAVESLMLAGVSRMDALKTVARERGLSKRDIYRLVNGNRGP
                     KTK"
     gene            complement(1162..2325)
                     /locus_tag="VG274_11275"
     CDS             complement(1162..2325)
                     /locus_tag="VG274_11275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE07282.1"
                     /translation="MTITSLLSDACYEMVLAVLPGFLPIIHVAAAALGWIEGASDAFS
                     SFLKLFAGWYSDRIGQRKRMVVLGYLFTGTGLSIFSVASTWIPIFLGRLVSWFGKGIR
                     GSLRDAMLSESVDPAVRGRAFGFHRAGDTVGAIVGPLAGVALLSWLPKPSPDIPFREI
                     FLFSLIPGLAAPIAFALLVHETRRQPRPGLKLASSLRELPAAFRKFLVGVGIFGCGDF
                     SPTLLTLAAATLLRAQHGALRAAELAALLYVIRNAVYAAASWPIGAIADRAPKIPLLA
                     AGFLCAAAVAAWTGILFNGHYASFAVLAGVFSLSGIFAAAQDTLQGAIPPDLTSPESR
                     GTVYGTLGAVNGIGDLAASALVGTLWTAVAPSIAFFSAAAIMAVGALALLASL"
BASE COUNT          495 a          644 c          923 g          416 t
ORIGIN      
        1 accagcactt cacaggcgac gcggccatag ggatcctgct cgagaacggc gtcaaggacg
       61 gcatccgaga tctggtcggc aattttgtca ggatgcccct ccgtgaccga ttcggacgta
      121 aaaagatgct tttgcgcttc tgccatgcac actccggttt gatggatgga accaggctat
      181 ttcgatttgc cctgcagaga acggctgaag agtttcaggc cggtcaatac aaactcttag
      241 tttacatcgg tagtgtgtgg cgcccggccg cagcgttcca gtaccgggaa gcgggcaagt
      301 agcattacag ctatttcgtt ttcggccccc gattgccgtt cacgagacgg taaatgtccc
      361 gtttcgaaag gccgcgttcc cgggcgacgg ttttgagagc gtccatgcgg ctgacgccgg
      421 cgagcatcag ggattcgacc gcctgatcga ccggtgtttc gtcgccggcg ggcttcatcg
      481 ctttcgcgat gaggatgacg aattcgccgc ggatggcatc gcgcgcggag agagtctggc
      541 ggatggaggc ggcggttcca cggaggagtt cctcgtgaat tttggtgagc tcgcgggcga
      601 cagcgatttc gcgatcgcgc aggacggatt cgatatcggc cagagtttcg agaatgcgat
      661 gcggcgcttc gtagaagatg agcgtcgccg gttcgtcgcg cagggattcg aggacgcgga
      721 tgcgctgcga ttctttcggg gggaaaaagc cggcgaagtg gaattgatcg gtgggcaggc
      781 cggaagcgac gagcgcggtg aggagcgcgg atgggccggg gagggcttcg accgtgatgc
      841 ccgattcgac cgcggcgcgg acgatgcgat agccagggtc agagagaagc ggggtgccgg
      901 catccgagac gagagcgatc gacacgcccg attcgaggcg ggcgatgagt tctttggcgc
      961 gctcctgctc gttgtgttcg tggtagctga tggcgccggt tgaaatgccg tagtgatcga
     1021 ggaggcggcg cgtggtgcgg gtgtcttcgc aggcgatgac gtccgcttcg ccaaggattc
     1081 ggaccgcccg ataggtgatg tcttcgagat ttccgatggg ggtggaaacg atgtagaggc
     1141 ggccgggcac ggctgagttg attataagga cgcaaggagg gctaacgccc ctaccgccat
     1201 gatggcggcg gcgctgaaga aggcgatgga gggagcgacg gcggtccaga gagtgccgac
     1261 cagggcgctg gcggcgaggt cgccgatgcc gttcacggct ccgagagtgc catacacggt
     1321 gccgcgcgat tcggggctgg tgagatcggg cgggatggcg ccctgaagag tgtcctgggc
     1381 ggcggcgaag ataccggaga gggaaaaaac gcccgcgagc acggcgaagc tggcgtagtg
     1441 gccgttgaag agaatgccgg tccacgccgc cacggcggcg gcgcagagga atccggcggc
     1501 gaggagcggg attttggggg cacggtccgc gatggcgcca atgggccagg aagcggcggc
     1561 ataaaccgcg ttgcggatta cgtaaagcag ggccgcgagt tcggcggcgc gcagggctcc
     1621 gtgttgggcg cggaggagcg tggcggcggc gagggtgagc agtgtgggag agaaatcgcc
     1681 gcagccgaag atgccaacgc ccacgaggaa tttgcggaag gccgccggga gctcgcggag
     1741 cgatgacgcc agcttcaggc cgggacgcgg ctggcggcgt gtttcatgga ccagcagagc
     1801 gaaagcgatg ggggcggcga ggccggggat cagggaaaag aggaagattt cgcggaaagg
     1861 aatgtcgggg gacggtttcg gcagccatga cagcagcgcg acgcccgcga gcgggccgac
     1921 gattgcgccc accgtgtcgc ccgcgcgatg aaagccaaaa gcgcggccgc gaacggcggg
     1981 atcgaccgat tcggaaagca tggcgtcgcg cagcgagccg cggatgcctt tgccgaacca
     2041 gctcacgagg cggccgagga agatggggat ccaggtggaa gcgacggaaa agatggagag
     2101 cccggttcca gtgaagagat aaccgaggac gaccatgcgc ttgcgctggc cgatgcggtc
     2161 ggaataccag ccggcgaaga gtttcagaaa gctggagaag gcgtccgagg cgccttcgat
     2221 ccagccgagg gccgccgcgg ctacgtggat gattggcagg aagccgggca gaacggccag
     2281 caccatttcg taacaggcgt ccgagagcag actggtgatg gtcatgcccc agacgctacg
     2341 gtttagccag cgcggattcc ccactttccg atgttcacat ggcggcgcga aaccgaactc
     2401 ggagtctttt ggtccgcgtt cctgatacaa tcggatcgaa acaaagtttg tccttatgat
     2461 cgatacgttt gtgatcac
//
LOCUS       DASZMC010000557         4286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2704678,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000557 DASZMC010000000
VERSION     DASZMC010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704678"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1152)
                     /gene="purM"
                     /locus_tag="VG274_11280"
     CDS             complement(79..1152)
                     /gene="purM"
                     /locus_tag="VG274_11280"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016137333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWE07283.1"
                     /translation="MASDERSRRNGLTYRQAGVDIDAADALIARIAPAARATRRAGAD
                     AELGGFGGLFDLKAAGFKDPILVASTDGVGTKLRLAIEARDFSTIGQDLVAMCVNDIV
                     VQGAEPLFFLDYYATGKLAIDDAAVLIEGIARACKESGCALIGGETAEMPGLYAQGDF
                     DLAGFSVGAVERDGVLPKPADMRAGDVLIGVASSGLHSNGFSLVRRILSDASLVCAAP
                     APFARELNLGKILLAPTRLYVKSALAAIREGGVKGFAHITGGGISENLPRVLPDGLDA
                     EIDLAAWERAPLFAWLARAGGIEDAEMLRTFNCGIGLIAVVGPAQAQTCIDALSASGE
                     SARVIGKLVAGSGEPQVRYTGAL"
     gene            1515..2540
                     /locus_tag="VG274_11285"
     CDS             1515..2540
                     /locus_tag="VG274_11285"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HWE07284.1"
                     /translation="MGLTGVRAAGDPYTISNIAVDASAPSAVEAQTLAINSGRDKAWQ
                     ALYRKLTKQQDWGHQPVLDPVTLQRMVRSYAVSKERRSTTRFVASMTYVFNPEAVRRV
                     LRQANVAFADVAAKPLLVIPMSPGYAPHTPWAQAFADPHIAKGSVPLLLPLGDAVDSS
                     ALGALQFGNASWQDIEPAASRMHATEAWLALAEPGTGHITIKLKRLGAGASPSIPDVN
                     VTVPPKTPPAQAFASAANAAAAAIVDAWKAHSVVDFGKKAKLFAALHVDSLEDWSEML
                     KRLGSASNVTNVDVVALDIGEARVAISYVGSPDQLNDQLAHAGLSLASADGQMWLSRA
                     SSSAATP"
     gene            2546..3097
                     /locus_tag="VG274_11290"
     CDS             2546..3097
                     /locus_tag="VG274_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWE07285.1"
                     /translation="MLKHLPNLLTGLRLAAAPALALLLFAGADRAALGVFAFAGLSDA
                     ADGFLAKRFGFATRFGRFLDPAADKFLMFASFVTLSLMNVTPLWLTALVIARDLAIVF
                     GILVARVLALPIRVEPLLLGKASTAMQIVYVALALVLLTFGLHWTLLENIAADMTAVV
                     TVASWLAYAGVGARALARRRSTA"
     gene            3094..3759
                     /locus_tag="VG274_11295"
     CDS             3094..3759
                     /locus_tag="VG274_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07286.1"
                     /translation="MKPSQLPLPLSPAPQLSRADLIVSPANAEAVAFIDSWPGWSVAT
                     AALYGPQGCGKTHLVAIWSGMSDATLVSAREFSANLPRGPLAIEDVDSTPVTLARDSV
                     LFAALEAANSTAPLLLTGRQPPASWAHELPDLASRFAALAAFPLREPGEDVLAALTHK
                     LFADRQLPVPDAVISRMLHLLERSPGAIRDFVARADAAALAQSRPVNLSLIRDLIAEE
                     EGA"
     gene            3900..>4286
                     /locus_tag="VG274_11300"
     CDS             3900..>4286
                     /locus_tag="VG274_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07287.1"
                     /translation="MNAESLARMENRLLPLEEELAAAEGAPTPTIDANSPERFINREL
                     SWLAFNRRVLEEARNLRHPLLERLRFLSISAANLDEFFMVRVAGLYGQWRSGVTTPSQ
                     DGLAPADQLAQIRTRVAVLTDEQQQRW"
BASE COUNT          698 a         1459 c         1364 g          765 t
ORIGIN      
        1 cgctccgatg ggcgcgcgca cgtccttcaa cctccccaca agggggaggt gaagtgacga
       61 agtcgcgccg cctcgttgtc acagcgcgcc cgtgtagcgg acctggggtt cgcccgaacc
      121 cgcgacgagt ttgccgatga cgcgggcgga ttcgccggaa gcgctgaggg catcgatgca
      181 ggtttgcgcc tgcgcgggtc cgaccaccgc aatcaggccg atgccgcaat tgaaggtgcg
      241 cagcatctcc gcatcctcga tgccgcccgc gcgcgcgagc caggcgaaga gcggcgcgcg
      301 ttcccacgcc gcaagatcga tttccgcgtc gagaccatcg ggcaacacac gcggcagatt
      361 ctcgctgatg ccgccgccgg taatatgggc gaagcctttc acgccgcctt cgcgaatggc
      421 tgcgagcgcg ctcttcacat aaagccgcgt cggtgcgagc aggattttgc cgagattcag
      481 ctcgcgcgcg aaaggcgccg gcgcggcgca gacgagggag gcgtcggaca gaatccgccg
      541 caccagggag aaaccgttcg aatgcagtcc cgaagacgcg acgccgatca gcacatcgcc
      601 cgcgcgcatg tccgccggtt tcggcagcac gccatcgcgt tcgaccgcgc cgaccgaaaa
      661 tcctgccaga tcgaaatcgc cttgcgcata aagccccggc atctccgccg tctcgccgcc
      721 gatcagcgcg cagccgcttt ccttgcaggc gcgcgcgatg ccttcgatca gcaccgcagc
      781 atcgtcgatc gcgagttttc ccgtcgcgta gtaatcgaga aagaacaacg gctccgcgcc
      841 ctgcacgacg atgtcgttca cgcacatcgc cacgaggtcc tggccgatgg tggagaaatc
      901 gcgcgcctcg atggcgagac ggagcttggt gccgacgcca tcggtagagg cgacgaggat
      961 ggggtccttg aatccggccg ccttgagatc gaacaaacct ccgaagccgc ccagctccgc
     1021 atccgcgccg gcgcgccgcg tcgctctggc cgcgggcgcg atgcgcgcga tcaacgcgtc
     1081 ggcggcgtcg atgtcgactc ccgcctgccg ataggtcagc ccattgcggc gcgatcgttc
     1141 gtcagaggcc atggcgtcca agatcaggtg ccgacatttc gtctagcctt cccttaaggc
     1201 gcgcgtaaag aaaaagcggc gcacgtgatc cccccctcac ccgcttcgga gctgaattca
     1261 acgcccgtaa ctgcatccct ctcccgcaag gagagagggg aatcggagtc ctgccccgat
     1321 ggtagatcag cgcgcggagt cccctctccc ctcgtgggag aggggttaaa gaggacacat
     1381 ttcaacatca ccggcgtggg tgagggggaa actccgccgc ttcccgcctt ccggaaccag
     1441 ccgcccgccc ctatagtccc gctcgccagc agggtcccgc gatgcgcctt ctccttataa
     1501 tatgtgtatt ggctgtgggg ctgaccggcg tccgggcggc gggcgacccc tacacgatca
     1561 gcaatatcgc cgtcgacgcg tccgcaccct ctgcggtcga ggcccagacg ttggccatca
     1621 acagcggccg cgacaaggcg tggcaggccc tctaccgcaa gctcaccaag cagcaggatt
     1681 gggggcacca gccggtgctc gatcccgtca ccctccagcg gatggtccgt tcttatgcgg
     1741 tgagcaagga gcgccgctcc accacgcgct ttgtcgccag catgacctat gtgttcaacc
     1801 cggaggcggt acgccgggtg cttcgccagg cgaatgtcgc ctttgccgat gtggcggcga
     1861 agcccctcct ggtcatcccg atgtcgcccg gctatgcgcc gcacacgccc tgggcgcagg
     1921 ctttcgccga tccgcacatt gcaaagggga gcgtgccgct gctgctgccg ctcggcgacg
     1981 ccgtcgattc ttccgcgctc ggcgcgctgc agttcggcaa tgcgagctgg caggacatcg
     2041 agcccgccgc gtcgcgcatg cacgcgacgg aggcctggct tgccttggcg gagccgggca
     2101 cgggacacat caccatcaag ctcaagcggc tcggcgcggg cgcttcgccc tccattcccg
     2161 atgtcaacgt cacggtgccg cccaaaactc cgcccgcgca ggcctttgcg agcgcggcca
     2221 atgcggccgc ggctgccatc gtcgatgcgt ggaaagcgca ttcggtcgtc gatttcggca
     2281 agaaggcaaa actcttcgcc gcgctgcacg tcgattcgct cgaggactgg agcgagatgc
     2341 tgaagaggct cggctcggcc tcgaacgtca ccaatgtcga tgtcgtcgca ctggatatcg
     2401 gcgaggccag agtggcgatc tcctatgtgg gctcgccgga ccagctgaac gatcagctcg
     2461 cccatgcggg gttgagcctg gcaagcgcgg acgggcagat gtggctgagc cgcgcgagtt
     2521 ccagcgcggc cactccgtga gccgcatgct gaagcacctg ccgaacctgc tcacgggatt
     2581 gaggcttgct gccgctcctg cgctggcgct gcttctgttt gccggcgccg atcgcgcggc
     2641 gctcggcgtg tttgctttcg cggggttgag cgacgcggcg gacggtttcc tcgccaagcg
     2701 tttcggtttc gcaacgcgtt tcgggcgatt tctcgatccg gcggccgaca agtttctgat
     2761 gttcgcgtcg ttcgtcacgc tctcgctgat gaacgtcacg ccgctgtggc tgaccgcgct
     2821 ggtcatcgcg cgcgatctcg cgatcgtttt cgggattctc gtcgcgcgtg tgcttgcgct
     2881 gccgattcgc gtcgagccgc tgttgctcgg caaggcgagc actgcgatgc agatcgtcta
     2941 tgtcgcactt gcgctcgtgc tcctcacctt cggcctgcac tggacgctgc tggaaaacat
     3001 cgccgcggac atgacggcgg tcgtcaccgt cgcgtcgtgg cttgcctatg ccggcgtggg
     3061 cgcgcgggca ttggcgcgcc gccgcagcac cgcgtgaagc cgtcgcagct ccccctgccg
     3121 ctgtcgccgg cccctcagct ttcgcgcgcc gatctgatcg tctcgccggc aaacgcggaa
     3181 gcggttgcct tcatcgattc ctggccggga tggtctgttg caacggcagc gctctacggc
     3241 ccgcaaggtt gcggcaaaac gcatctcgtc gcgatctgga gcgggatgtc ggatgcaacg
     3301 ctggtttccg cgcgcgagtt ttccgcaaat cttccgcgcg ggccgctcgc aatcgaagat
     3361 gtcgattcca cgccggtgac gctcgcgcgc gactcggttt tgttcgcggc gctcgaagcg
     3421 gcaaactcca ccgcgccgtt gcttctgacc ggacggcaac cgcccgcatc atgggcgcat
     3481 gagcttcccg atcttgcttc gcgctttgcg gcacttgccg cgtttccgct gcgggagcct
     3541 ggcgaggacg tgctcgcggc tctcacgcac aaattgttcg cggaccgcca acttcccgtg
     3601 cccgatgcgg tgatctcccg catgctgcat ctgctcgagc gctcgccggg cgcgatccgc
     3661 gatttcgtcg cccgcgccga cgcggcagcc ctcgcgcaat cgcgccccgt caatctctcc
     3721 ctcatacggg acctgatcgc agaggaagaa ggggcctgag gcgcgaacat gcatctatcc
     3781 tccccttgag ggagggtgga accacactgg tggatgaggg gaaccgccat gacagattca
     3841 tatatttgct gtaggctctt gcccatgaat ccagaagcaa atgccatggc ggccagccga
     3901 tgaacgccga aagccttgcc cggatggaaa atcgcctgct gccgctcgag gaagagttgg
     3961 ccgcggcgga gggcgcgccc acgcccacga tcgacgcgaa ttcgcccgag cgcttcatca
     4021 atcgcgagct gtcctggctg gccttcaacc ggcgcgtgct ggaagaggcg cggaacctcc
     4081 gccacccgtt gctcgaacgc ctgcgcttcc tgtcgatctc ggccgccaat ctcgatgaat
     4141 tcttcatggt gcgcgtggcc ggactatacg gccagtggcg gtccggcgtg acgactccgt
     4201 cgcaggacgg gttggcccca gccgatcaac tcgcccagat tcgcactcgc gtcgccgtgt
     4261 tgacagacga gcagcagcag cgttgg
//
LOCUS       DASZMC010000558         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2976513,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000558 DASZMC010000000
VERSION     DASZMC010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976513"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1691)
                     /locus_tag="VG274_11305"
     CDS             complement(165..1691)
                     /locus_tag="VG274_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWE07288.1"
                     /translation="MLARQAMAQTILIVDDDPVQRRLLEAAITRSGMAVVTAPGGQPA
                     LDLLNGPRGDQFTLVLLDLVMPDMDGLAVLARLKVSHHDLPVIVLTARGGIDSAVEAM
                     RAGASDFLVKPASPERIAVSIRNALKIGALAGEVSRLKKRADNRLDFDDLIGVSGEMH
                     QVVRLGRRAAQSNIPILIEGESGVGKEVIARAIQGSSERAGRPFVTVNCGAIPENLIE
                     SILFGHEKGSFTGATDKHLGKFQEADGGTLFLDEIGELRLDMQVKLLRALQEGEVDPV
                     GSKRPVKVDVRIVSATNRDLAERMRAGLFREDLYYRLNVFPVHVPALRQRREDIAELA
                     RYFIERFAAEENKPIAGMTAEASTLIERFDWPGNVRQLENTIFRAVVLCDGDTLDICD
                     FPQIAAAMGIEAKPRRLPVAAPAIHIVESVEEFVHVPAVSTFAIPATNETGHMRRLED
                     IESEVIRMAIDHYDGHMSEIARRLGIGRSTLYRKLRELGLDQTESVHELEDERVREAV
                     "
BASE COUNT          375 a          625 c          633 g          384 t
ORIGIN      
        1 tctagataaa aaaattttag cgcagagggc gcagagggtg cgcgggggac gcggggaaaa
       61 tcgcgcgccg gaggcgcgta gttcgtttac tgcgtcctcc gcgcaccctc tgcgtcctct
      121 gcgctaaaga gttttggttg ctcgactgca tcacgactcg gtcgtcaaac ggcttcgcgg
      181 acgcgctcgt cttccagttc gtggacgctt tcggtctgat cgagccccaa ctcccgcagc
      241 tttcgataga gagtcgagcg tccgatgccc agtcggcggg cgatttccga catgtggccg
      301 tcataatgat cgatggccat gcggatcact tccgactcga tgtcttcaag cctgcgcatg
      361 tgaccggtct cgttggtggc ggggatcgcg aacgtggaga cggcgggcac gtgaacaaat
      421 tcttccacgg attccacgat atgaatcgcc ggagccgcca ccggcagccg cctcggcttc
      481 gcctcgatgc ccatcgcagc ggcgatctgc ggaaaatcgc agatatcgag cgtgtcgccg
      541 tcgcaaagca cgacggcgcg gaagatcgtg ttttcaagct ggcggacgtt tccgggccaa
      601 tcgaatcgct cgatcagcgt gcttgcctca gccgtcatgc cggcgatggg cttgttttct
      661 tccgcagcga atcgttcgat gaaatatcgc gccagttccg cgatgtcctc gcgccgctgc
      721 cgaagtgccg gaacgtggac cgggaagaca ttcagccggt aatagagatc ctcgcgaaac
      781 agacccgcgc gcatacgctc tgcgagatcg cgattggtcg ccgaaacgat gcggacgtcg
      841 accttcaccg ggcgcttgga gccgacggga tcgacctctc cttcctgcaa cgcgcgcaac
      901 agcttgacct gcatgtcgag gcgaagctcg ccgatctcgt cgaggaacag cgttccgccg
      961 tcggcttcct ggaacttgcc aagatgtttg tcggtcgcgc cggtgaacga acctttctcg
     1021 tggccgaaca gaatcgactc tatgagattc tcgggaatgg cgccgcagtt cacggtgacg
     1081 aagggccgcc ccgcgcgctc ggaagaaccc tggatggcgc gggcgatgac ctccttgccg
     1141 actccggatt ctccttcgat gaggatcgga atgttcgact gcgccgcccg ccggcccagg
     1201 cggacgacct gatgcatctc gccgctgaca ccgatgagat cgtcgaaatc gagacggttg
     1261 tcagcacgtt tcttgagtct cgacacttca cccgcgagcg cgccgatctt gagtgcgttc
     1321 ctgatggaaa ccgcgattcg ctcggggctt gccggcttca ccagaaaatc gctcgcgccc
     1381 gcgcgcatcg cctcgacggc ggaatcgatt ccgcccctgg ccgtcagcac gatgacggga
     1441 agatcgtgat gactgacctt gagccgcgcg agcaccgcca ggccgtccat gtcgggcatg
     1501 acgagatcga ggagcacgag cgtgaactgg tcgccccgcg gaccgttcag caggtcgagc
     1561 gccggttggc caccgggcgc ggtgacgacc gccataccgg agcgcgtaat cgccgcttcg
     1621 agcagcctgc gctggaccgg atcgtcgtcg acgataagta tcgtttgtgc catcgcttgc
     1681 cttgccagca tggtcactcc ggaagggtaa aagcgggttt aaggtcccgc tttgcgacac
     1741 caccatccgg agatgatatg caagctgtgc cgatttcggt cgcatttcgc gatgggaatg
     1801 gccgatcgta gtgaagaaac actgaaggcg gaatgcggcg tgaaaccatg aaagaccgcg
     1861 aaaacggctt cgcgcgccgg ctgacgcgct tcgcgcgcgt ttccgccggc ctgaccggcg
     1921 ttgccgcgcg gggcgccgga cgggcactca atggaacacg catcttcagc ccgtcgaatg
     1981 cggccgacct gacccaggtg ctcggcaatc tgcgcgg
//
LOCUS       DASZMC010000559         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4903011,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000559 DASZMC010000000
VERSION     DASZMC010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4903011"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..325)
                     /locus_tag="VG274_11310"
     CDS             complement(101..325)
                     /locus_tag="VG274_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWE07289.1"
                     /translation="MKVYGADWCPLTRRAIDHLKRRGIPFEYINLEEDPRAAEWVKAQ
                     NGGKEKKPTIDLDGQILSEPTNDELDAALA"
     gene            complement(363..1364)
                     /locus_tag="VG274_11315"
     CDS             complement(363..1364)
                     /locus_tag="VG274_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWE07290.1"
                     /translation="MEIERAGHVLTAREIFDLVREDLEAVEKAIDIESIASVDTVTTI
                     GRYLHQSGGKRLRPALLLLCARFAGGGGKTAVRLGAVVEMIHAATLVHDDVIDVARTR
                     RGRPSANVQWGNHTCVLAGDWLYMQAFQVALRERNFHVLDLLISLTQMMVEGELIQLD
                     RIGRIDITEADCMELVDRKTACLFSACARLGAVAAGADTSLEEKLGEFAWNLGMAFQL
                     IDDTLDFTSREQTLGKPVGGDLKEGKVTLPLVYALEGAGAAEGQQVEKVLQDRSYDAV
                     PFDQILTFVEARGGLRRTRERAQQFRDRALQIVSEFPDTPYQRALVTVTEMVTERDR"
     gene            complement(1354..2163)
                     /locus_tag="VG274_11320"
     CDS             complement(1354..2163)
                     /locus_tag="VG274_11320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWE07291.1"
                     /translation="MTLTDSHCHLDDKKYAQDLDKVLARAAEAGVARMLTIGTGDGPP
                     ELDRAVRLAEKYDQLFASIGVHPHDAAKCEPRSFDDLCALGRHSKVVAFGEIGLDYHY
                     DFSPREIQREVFAAQLKIARELDLPVIIHTREAWEDTLNLLRQHWSGPGIMHCFTGDS
                     AQAEQALELGFHLAFGGVITFRTAESVREAAAVVPDERLLIETDAPYLAPIPWRGKRN
                     EPAMMVETAKTLAALRNTTPERIAEITSGNFELLCLRPRNVNRYTEVSDGD"
BASE COUNT          430 a          643 c          732 g          451 t
ORIGIN      
        1 cgattgggat tcggcgaaga tgaaattcac gaacaacgcg gaagcgactg agatggtgcg
       61 gccgaagttc cgaaaagggt ggggctggag ctgatcggct tcaggcgagc gccgcgtcga
      121 gctcgtcgtt cgtcggttcc gagagaattt gcccatcgag atcgatggtg ggcttttttt
      181 ctttcccgcc attctgagct ttcacccatt cagcggcgcg agggtcttct tcaagattga
      241 tgtattcgaa cgggatgcct cgccgtttta aatgatcgat ggcgcgccgc gtcaatgggc
      301 accagtcggc tccgtaaact ttcaccttcg tcacattccg ataaggagca atttgacagc
      361 cgtcagcggt cgcgttccgt caccatttcg gtaacggtga cgagagctcg ttgatacggc
      421 gtatccggaa attcggaaac gatctggagc gcgcggtcgc ggaactgctg ggcgcgctcg
      481 cgagttcggc gcaatccgcc gcgcgcttcc acgaaggtca aaatctgatc gaagggaacg
      541 gcgtcgtagc ttcggtcctg aagcactttt tccacctgct ggccctcggc cgcgcctgcg
      601 ccttcgagcg catagacaag gggtaatgtc accttgcctt ctttgagatc gccgccgacc
      661 ggtttgccca gcgtctgttc gcgcgaagtg aaatcgagcg tgtcatcaat aagctggaac
      721 gccattccga gattccaggc gaactcgccg agcttctctt cgaggctggt atcggcgccg
      781 gcggccacgg ctccaaggcg cgcgcacgcg gaaaagaggc aggcggtttt gcggtccacg
      841 agttccatgc aatcggcctc ggtgatgtcg atgcggccga tgcgatcgag ctggatcaac
      901 tcgccttcga ccatcatctg ggtgaggctg atgagcagat cgagaacatg aaaatttcgc
      961 tcgcgcaacg ccacctggaa ggcctgcata taaagccaat cgcccgcgag aacacaggtg
     1021 tggttgcccc actggacatt ggcggaaggg cgtccgcggc gggtgcgcgc aacgtcgatc
     1081 acgtcatcgt gaaccagcgt ggcggcgtgg atcatttcca caacggctcc gaggcgaacg
     1141 gcggtttttc cgccgccccc ggcgaagcgc gcgcaaagca gaagcagcgc ggggcggagg
     1201 cgtttgccgc cactctggtg aagataacgc ccgatggtgg tgacggtgtc gaccgaagcg
     1261 atggattcga tatcgatcgc tttttcgacc gcctccaaat cttcgcgcac gaggtcgaat
     1321 atctccctcg cggtcagcac gtggcctgcg cgttcaatct ccatcggaaa cctcagtgta
     1381 acggttcaca tttcgtggcc gcaaacacag cagttcgaag ttgccggatg tgatctcggc
     1441 aatgcgctcc ggcgtggtgt ttcgcagcgc ggcgagggtt ttagccgttt ccaccatcat
     1501 ggccggttcg ttgcgcttgc cccgccaggg gatgggcgcg aggtaaggcg cgtcggtttc
     1561 gatgagcagc cgctcgtccg gcacgacggc ggcggcttcg cgcacggatt cggccgtgcg
     1621 aaaggtgatg acgccgccga aagcgagatg aaagccgagt tcaagcgcct gttcggcctg
     1681 agcgctatcg cccgtgaagc aatgcatgat gcccgggccg gaccagtgct ggcgaagcag
     1741 gttcaatgtg tcttcccagg cttcgcgggt gtgaatgatc accggcaagt cgagttcgcg
     1801 ggcgattttc agttgggcgg cgaagacctc gcgctggatt tcgcggggtg agaaatcgta
     1861 gtgataatcg aggccgattt cgccgaatgc gacgacctta gagtggcggc cgagggcgca
     1921 cagatcatcg aaactgcgcg gctcgcattt ggcggcgtcg tgcgggtgaa cgccgatgga
     1981 ggcgaacaac tggtcgtatt tctcggccag gcggacggca cggtcaagtt ccggaggacc
     2041 gtcgcccgta ccaatggtga gcatgcgtgc aacgccggct tcggcggcgc gagcgagtac
     2101 cttgtcgaga tcctgcgcgt actttttatc gtcgagatgg caatgggaat cggtcagcgt
     2161 catttcagac gcgcgccgac cggcacgtcc tcgagaaacc cggcgagcac gggcggaccc
     2221 ccttcgagcg aggcggcgac gatcatgccg ttggac
//
LOCUS       DASZMC010000560         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3779517,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000560 DASZMC010000000
VERSION     DASZMC010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3779517"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VG274_11325"
     CDS             <1..402
                     /locus_tag="VG274_11325"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HWE07292.1"
                     /translation="SHFGLGFGPMDRLRRKFSGNIHQVSAVFAGVNRRVDSQTFEGAD
                     LNSTFGELKVDLRGATISTPNRQATIRVNAAFGAIKLRVPETWRVIVHGDAVFGAFQD
                     KTVPPRPTPGLDPPTLFIVGNSAFGGVEIEN"
     gene            412..1443
                     /locus_tag="VG274_11330"
     CDS             412..1443
                     /locus_tag="VG274_11330"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWE07293.1"
                     /translation="MHPLLSNWRRILLYLTGWIEFGVVLGLIVPASSGLRRPEAVAVV
                     VPLMLVIGIVCLAPWYICRSVPLRGIGLRSVLQHHILGAVIATVVVTVIGRILASMLA
                     SPWPGLDNLFSKAIPVITIFVALTYMLSTSLHYAFLEIESSRRAEILAREAQLRALKA
                     QINPHFLFNSLNSISALTSLDPARAREMCIGLADFLRASLRLGERDTVPFGEEIALTN
                     MYLSVEKARFGDRLRLIRDIDSACESCEVPALIVQPLVENAVKHGIAMMAEGGEISIV
                     SRCEHDQLRFSVSNPFDPEAPVQDRNGIGLRNVRARLEARYGAAAKMQIDATDSSYQV
                     TLTIPAKSI"
     gene            1440..2195
                     /locus_tag="VG274_11335"
     CDS             1440..2195
                     /locus_tag="VG274_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE07294.1"
                     /translation="MKTLRAIIADDEELARRILREYLAGEADIEIIQECPNGFEAVKA
                     VNDLKPDVLFLDVQMPRLDGFEVLELVDTSVAVVFVTAFDQYAMKAFDAAAVDYLLKP
                     FTVERFKAALDRVRTKLNTSAPPEPLPRDLKLASRAPGEYLERLVVKDGPRVHVIPAA
                     KLDYAEAQDDYVALRSEGKTWLKQQTISGLESALDPRRFLRVHRGFLINMERIARVEP
                     NTKDTWLAILADGTKIPMSRAGYSRFRELADGN"
     gene            2225..2482
                     /locus_tag="VG274_11340"
     CDS             2225..2482
                     /locus_tag="VG274_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07295.1"
                     /translation="MPARKAAVRQSITLPGGVASEVRAMARKRHLSANRVILELIEGG
                     IEAEKRRQQEFFELAGRFRDATDPAEIQRLGDEMGRMVFGG"
     gene            2566..2727
                     /locus_tag="VG274_11345"
     CDS             2566..2727
                     /locus_tag="VG274_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07296.1"
                     /translation="MADLNQLRLWIDSHPEVPTGEWYKDFGTFKICGEGALPKTFLLR
                     GQAAHGEKI"
     gene            complement(2763..>3052)
                     /locus_tag="VG274_11350"
     CDS             complement(2763..>3052)
                     /locus_tag="VG274_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07297.1"
                     /translation="NFTSSSSTITTAVGDTLNGTFLQDKSGAVDSIDGTINGEFVVNG
                     NISLLSNGTLKGLGFTATPEPGSFTLGLVSCFGLAGLVFARRRRNRVSAIG"
BASE COUNT          610 a         1058 c          811 g          573 t
ORIGIN      
        1 agtcatttcg gtctcggttt cggacccatg gatcgtctgc gccgcaagtt ctccggcaac
       61 atccaccagg tgagcgcggt cttcgccggc gtcaaccgcc gcgtcgattc gcagactttc
      121 gaaggcgccg atctcaacag caccttcggc gagctcaaag tcgacctccg tggcgccacc
      181 atctccacgc ctaaccgcca ggccaccatt cgcgtcaacg cggcgttcgg cgccatcaag
      241 ctccgcgtgc cggaaacctg gcgagtgatc gtgcatggcg atgccgtctt cggcgccttc
      301 caggacaaaa ccgtcccgcc gcgacccacg cctggcctcg acccgcccac gctcttcatc
      361 gtcgggaact cggcgttcgg cggcgttgaa atcgaaaact gatccgccgc catgcaccct
      421 ctcctcagca actggcggcg gatcctgctc tacctcaccg ggtggatcga attcggtgtg
      481 gtcctcggcc tgatcgttcc cgcgtcctcc ggactccggc gtccggaagc ggtggccgtc
      541 gttgttccat tgatgctcgt gatcggaatc gtgtgcctcg ccccctggta catctgccgc
      601 tccgtcccat tgcgcggcat tggactgcga agcgttctgc agcaccacat cctcggcgcg
      661 gtgattgcaa cagtcgtcgt gaccgtcatc ggccgcatcc tcgccagcat gctcgcctcg
      721 ccgtggcccg gccttgacaa cctcttctcg aaagccatcc cggtcatcac gatcttcgtt
      781 gcgctcacat acatgctttc cacctcgctc cactacgcgt ttctcgaaat cgaatcttcg
      841 cgacgcgccg aaatccttgc gcgtgaagcg cagctgagag cgctgaaagc ccaaatcaat
      901 ccgcacttcc tcttcaacag cctcaactcc atcagcgcgc tcacttcgct cgaccccgcc
      961 cgcgcccgcg aaatgtgcat cggcctcgcc gatttccttc gcgcctccct tcgtctcggc
     1021 gagcgcgaca cggttccgtt cggcgaagaa atcgccctca ccaacatgta tctgagcgta
     1081 gaaaaggcgc gcttcggaga tcgccttcgc ctcatccgcg acatcgattc cgcctgtgaa
     1141 tcctgcgaag ttccggccct catcgtgcag cctctcgtcg agaacgccgt caagcacggc
     1201 atcgccatga tggcggaagg cggcgagatt tccatcgtca gccgttgcga gcacgatcag
     1261 cttcgcttct ccgtttcgaa tccgttcgat cccgaagcgc ccgtgcagga ccgcaatggc
     1321 atcggcctgc gcaacgtgcg cgcccgcctc gaagcgcgct atggcgccgc cgcgaagatg
     1381 cagatcgacg ccacggattc cagctaccag gtcactctca ccataccggc caaatcaata
     1441 tgaaaaccct gcgcgccatc atcgccgacg atgaagaact cgcccgccgc attctccgcg
     1501 aatatctcgc cggcgaggcc gatatcgaaa tcattcagga gtgccccaac ggtttcgaag
     1561 ccgtcaaggc cgtcaacgat ctcaaacccg acgtcctgtt cctcgatgtc cagatgccga
     1621 ggctcgatgg tttcgaagta ctcgaactgg tggatacttc cgtcgccgtc gtcttcgtca
     1681 ccgcgttcga tcagtacgcg atgaaagcct tcgacgccgc agcggtcgat tatcttctga
     1741 agccgttcac cgtcgaacgt ttcaaagccg cgctcgatcg cgtgcgcaca aaactcaaca
     1801 cctccgcgcc gccggaaccg ctcccgcgcg atctgaagct cgcctcacgc gcgccgggcg
     1861 aatatctcga acggttggtt gtgaaagacg gtccgcgagt ccatgtcatc cccgccgcga
     1921 aactcgacta cgccgaagcg caggacgatt atgtcgccct tcgcagcgaa ggcaaaacct
     1981 ggctgaagca gcagacaatc tccggcctcg aatccgctct cgatccccgg cgcttccttc
     2041 gggtccaccg cggctttctc atcaacatgg agcgcatcgc ccgcgtggag ccgaacacga
     2101 aagacacctg gctcgccatt ctcgccgacg gcacgaaaat ccccatgagc cgcgcgggct
     2161 attccagatt ccgtgaactc gccgacggca actaacgcgc accggattgg tgcataatgg
     2221 tgacatgccc gcaagaaagg ctgcagtccg ccaaagcatc acactgcccg gcggtgtcgc
     2281 atcagaagtg cgcgccatgg cacgcaagcg tcacctgagc gcaaaccgag tcatcctcga
     2341 attgatcgaa ggcggaatcg aagcagagaa gcggagacag caggagtttt tcgaactcgc
     2401 tggccgcttc cgcgatgcaa ccgacccagc cgagatccag cgcctcggcg acgaaatggg
     2461 tcgaatggtt ttcggcggct aatgccgaaa atcaggatct gggcggagct ccccgaaggt
     2521 gtccgccgcc acctcatccg gcgaatgcac gagcgagccg tcagcatggc cgatctcaac
     2581 caacttcgct tgtggattga ctcccatccc gaagtcccaa ctggcgaatg gtacaaagac
     2641 ttcggaacgt tcaaaatttg cggcgaaggc gctctgccaa agacatttct tttgcgcggg
     2701 caggcagcgc acggcgaaaa aatctgatcc ggacttggaa cgaacgcgaa acgccgaacg
     2761 cgtcagccga tcgcgctcac ccggttcctg cggcgccgcg caaacaccaa ccccgccagc
     2821 ccgaagcacg aaaccagacc gagcgtgaaa ctacccggct ctggcgttgc cgtgaaaccc
     2881 aggcccttca gcgttccgtt cgaaagcagc gagatgttcc cgttcaccac aaactcgccg
     2941 ttgatcgtcc catcgatgct gtctacggca ccgctcttgt cctggaggaa agtcccgttc
     3001 aacgtgtcgc ccactgccgt ggtgatcgtc gaactcgagg acgtgaagtt at
//
LOCUS       DASZMC010000561         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1047311,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000561 DASZMC010000000
VERSION     DASZMC010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047311"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VG274_11355"
     CDS             complement(<1..330)
                     /locus_tag="VG274_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07298.1"
                     /translation="MRGHDNHRHVDPRLARAFQNRLLNRVMGANQSWNYAGNIAAALA
                     GMVLVSALGLESVFYSVGACALLAAVSVVLIRERDLDERVATGRTHEENAERSGRTCC
                     AIAYFSSS"
     gene            319..1143
                     /locus_tag="VG274_11360"
     CDS             319..1143
                     /locus_tag="VG274_11360"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWE07299.1"
                     /translation="MTAHGDSGSVIEAMRLGAYDFAAKPLDFEQLLTQLDRALSRRKI
                     SRELKSDGAAPEFSGKAAIIGYSPAMPRVYKLIGQVAASDATVLVRGESGTGKELVVN
                     AIHQNSLRAKGPLVKVNCAAIPETLLESELFGHEKGAFTNAIQRRVGRFEEANGGTLL
                     MDEIGELTPALQSKLLRVVQKRAIERLGASNVIRVDLRLVTATSRNLEEAVSQGQFRE
                     DLYYRLNVMVIELPPLRERKQDIPALVEYFLRRGGRNLSVTPAALNLLCDYHWPGM"
     gene            1161..1430
                     /locus_tag="VG274_11365"
     CDS             1161..1430
                     /locus_tag="VG274_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWE07300.1"
                     /translation="MERGAALAKGGGILDAGDIDLKSVARATGNWTDQIPLGDGWKAN
                     VAAVEKAMVARPLRMAAGKKSRAAESLGIHRRLLYEKMREHGVEG"
     gene            complement(1480..>1666)
                     /locus_tag="VG274_11370"
     CDS             complement(1480..>1666)
                     /locus_tag="VG274_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07301.1"
                     /translation="PPGILFEFDQTEHPFRDAIIRERLAMDPADGAVAVPEGPGLGVT
                     VIPEAVAEFRTGLFSIP"
BASE COUNT          339 a          438 c          614 g          275 t
ORIGIN      
        1 agaggaagag aaatacgcga tcgcgcagca ggtacgtcca gagcgctcgg cgttctcctc
       61 gtgcgtgcgg ccggtggcca cgcgttcatc gagatcgcgc tcgcgaatca ggaccacgga
      121 taccgccgcg agcagcgcgc acgcgcccac cgaatagaac acggattcga gaccgagcgc
      181 cgagaccagc accatgcccg ccagggcggc tgcgatattg cccgcgtagt tccagctctg
      241 gtttgcgccc atcacgcggt tcagcagacg attctgaaac gcgcgcgcga ggcggggatc
      301 gacgtgccgg tgattgtcat gaccgcgcat ggcgacagcg gctccgtcat agaagcgatg
      361 cggctgggcg cgtacgattt cgcggccaag cctctggatt tcgagcaact gcttacacaa
      421 ctcgatcgcg cgctgtcgcg gcggaagata tcgcgcgagc tgaagagcga tggcgcggcg
      481 ccggagtttt cgggcaaggc ggcgattatc ggttacagcc cggcgatgcc gcgggtatac
      541 aagctgatcg ggcaggtggc ggcgagcgat gcgacggtgc tggttcgcgg cgaatcgggc
      601 acgggcaagg aactggtggt aaacgcgatt caccagaaca gcctgcgcgc gaagggtccg
      661 ctggtgaagg tgaattgcgc ggcgataccg gagacgctgc tcgaatccga actgttcggg
      721 catgagaagg gcgcgtttac gaatgcgatc cagcggcgcg ttggccgctt cgaagaagcg
      781 aacggcggca cgctgttgat ggacgagatt ggcgagctga cgccggcgct gcaatcgaaa
      841 ctgctgcgag tggtacagaa gcgcgcgatc gagcggctgg gcgccagcaa cgtgattcgg
      901 gtggatctgc ggctggtgac ggcaacctcg cgcaatctgg aagaggcggt aagccagggg
      961 cagtttcgcg aggatcttta ttatcggttg aatgtgatgg tgattgagct gccgccgcta
     1021 agggaacgaa agcaggatat tccggccctg gtggagtatt ttctgcggcg cggaggacgg
     1081 aacctcagcg tcacgccggc cgcgctgaat ctgctttgcg attaccactg gccgggaatg
     1141 tgagggaact cgagaatacc gtggaacgcg gcgcggcgct ggcgaagggc ggcggcattc
     1201 tggacgcggg ggatatcgac ttgaaatcgg tagcgcgcgc aacggggaac tggacggacc
     1261 agattccact tggagatggg tggaaggcga atgtggcggc ggtggaaaaa gccatggtgg
     1321 cgcggccgct gcgcatggcg gcagggaaaa agtccagggc ggccgaaagt ctggggattc
     1381 acaggagatt gctttacgaa aagatgcggg agcacggggt ggaagggtga gggcagcgct
     1441 ggtttattgc tgacgagtct ccgggctggt tgcgccgggt caggggatcg aaaacaagcc
     1501 ggtacgaaat tccgcgaccg cctcggggat gacggttact cccaaaccgg ggccttcggg
     1561 aaccgcgacc gcgccatcag cgggatccat cgcgagccgc tcgcggatga tggcgtcgcg
     1621 gaacggatgt tcggtctggt cgaattcaaa gaggatgccg ggcgga
//
LOCUS       DASZMC010000562         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4369509,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000562 DASZMC010000000
VERSION     DASZMC010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4369509"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..944)
                     /locus_tag="VG274_11375"
     CDS             complement(72..944)
                     /locus_tag="VG274_11375"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HWE07302.1"
                     /translation="MGFMAWRPYVPVAVRHRNAQKKLARLKKRGQPAAPVIIAGRNIA
                     TSFWGKSWCENLESYSDYANRLPRGRTYVRNGSVVDLQIAKGEVAAFVCGSELYKVAI
                     AITPVAPHHWKAICRDCAGSIDSLVELLGGRLAKGVMERVCRKGGGLFPAPGAIKLSC
                     SCPDWADMCKHVAAALYGVGARLDEKPELLFVLRGVDEKDLLAAAAKHVPLAKKTPGA
                     AKLLDESDVADVFGIEMAASADAPRLNGKSPAPSKKSRPAKPGGKDKVRTTNYIKTAT
                     RVARTSPKSENRAS"
     gene            complement(948..3635)
                     /locus_tag="VG274_11380"
     CDS             complement(948..3635)
                     /locus_tag="VG274_11380"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWE07303.1"
                     /translation="MKLAPVLTPHGALILRRMEDAPELAGDPGARLEAAFARGSGHGL
                     LCLGADEVGTVLPPLLSYWRELGAQYLAGLCALPELSEGAAKPALPPPADGAFATMVL
                     SAPPMLGAEYLSAAVLDGLWRAIDAAFDVERSEARLPIADFLKARNAAWNLVGRVHFS
                     LAENRKDESAPFAFLATYTTRLSAQGRAQHLPLGRALQEYAGDRARLLSLLAPVQRAA
                     APCAWLRHMVDAGEIFHPLRWSAQDALQFLRDVPALESAGVVVRMPPNWRMNRPARPT
                     MRATVGGNAPSQLGMNALLDFRMELTLDGETLAPEEIELLLAQSDGLAFIRGKWVEVD
                     SARLARTIEQFEAVARRAGEEGLSFGEAMRLLARAPLSPDDISANAEIDWSETVAGPW
                     LAETLAGLRHGGTRALVDPGPSFHGTLRPYQRSGAHWMYLLSRLGLGACLADDMGLGK
                     TIQILSLLLVLKREAPGKPSLLVAPASLLANWSSEIDRFAPSVVAIVAHPSTPPESRP
                     RLDGSGDFSGADLVITSYGFLARAPWMVEIPWRLAILDEAQAIKNPAANQTRSVKRLN
                     AQARIALTGTPIENRLGDLWSIFDFLNPGLLGSSREFSAFVKSLAERAHNPYGPLREL
                     VSPYILRRLKTDRSIIADLPDKTEVRVFCPLSRRQAALYQEAVGDLAEALADKEGIER
                     RGIVLAFLLRLKQICNHPSHWLGDGAWAEADSGKFFRLRDIVEEIAARQEKALIFTQF
                     RETTAPLAAFLGSVFGRPGLVLTGETPVRTRKDLVRRFQEDENVPFFVLSLKAGGAGL
                     NLTGASHVIHFDRWWNPAVENQATDRAFRIGQTRNVLVHKFVCSGTVEDQIDRMIEAK
                     KELAGDLLGAGVDMQLTEMKDEDLLKLVTLDLNAAMKEG"
     gene            complement(3872..>4212)
                     /locus_tag="VG274_11385"
     CDS             complement(3872..>4212)
                     /locus_tag="VG274_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HWE07304.1"
                     /translation="AIDALSAGMPAGTLTGAPKIRAMEIIDEFETEKRGAAYAGAVGY
                     FAANGTMDTCIVLRTAVVKDGMMYVQAGGGVVADSVPQLEFEESVNKARALFSAADEA
                     ARYAALPRAE"
BASE COUNT          714 a         1369 c         1377 g          752 t
ORIGIN      
        1 gccgaaccgg ctgaggggct ccgtggccgc cgcgtctacg cataggcata cccgctttgc
       61 caggccatgc atcatgaagc gcggttctcg gattttggcg aagtgcgcgc aacacgtgtc
      121 gctgttttga tgtaattcgt cgtccgcact ttgtcttttc cgcctggctt tgccggtcga
      181 gacttttttg aaggggcagg ggacttgccg ttcaggcggg gcgcgtcggc ggaggcggcc
      241 atctcgattc cgaacacgtc ggcgacatcg ctctcgtcga gcagctttgc ggcgccgggc
      301 gtcttcttcg ccagcggcac atgctttgcg gcggcggcga gcagatcctt ctcgtcgacg
      361 ccgcgcagga cgaagagcag ttcgggcttc tcgtccagcc tcgcgccgac tccgtaaagc
      421 gcggcagcga cgtgcttgca catgtccgcc cagtccgggc agctgcagga gagcttgatc
      481 gcgccgggtg cgggaaagag gccgccgccc ttgcggcaga cgcgctccat gacgcctttg
      541 gccaggcggc cgccgagcaa ctccaccagc gaatcgatgg accccgcgca atcccggcag
      601 atggccttcc agtgatgcgg agccaccggg gtgatcgcga tggcgacctt gtagagttcc
      661 gagccgcaga caaaggctgc cacttcaccc tttgcgatct gcagatcgac taccgaacca
      721 tttcgcacat aggtgcgccc gcgcggcagg cgattggcat agtcgctgta gctttcaaga
      781 ttttcgcacc acgactttcc ccagaagctt gtcgcgatgt tgcggccggc aatgatcacg
      841 ggcgcggcgg gttgcccgcg tttcttcagt ctggcgagct tcttttgcgc gttgcggtgc
      901 cgcacggcaa ccggcacata gggacgccac gccatgaatc ccatcgctca gccctccttc
      961 atcgccgcgt tcagatcgag cgtcacgagc ttcagaaggt cttcgtcctt catctcggtc
     1021 agctgcatgt cgactccggc gcccagcaga tccccggcca attctttttt tgcctcgatc
     1081 atccggtcga tctggtcctc gacagtgccg ctgcagacga atttgtgcac cagcacgttg
     1141 cgagtctgcc cgattcgaaa cgccctgtct gtcgcctgat tctcgaccgc gggattccac
     1201 cagcggtcga aatgaatgac gtgagatgcg ccggtcagat tcaatcccgc gccgcccgcc
     1261 ttcaacgaca gcacgaagaa gggaacgttt tcgtcctcct ggaaccggcg cacgagatcc
     1321 ttgcgtgtcc gcaccggggt ctcccccgta agcacgaggc cggggcggcc gaacacggaa
     1381 ccgaggaacg cggcgagcgg cgccgtggtc tcccggaact gtgtgaagat cagcgccttt
     1441 tcctgacgcg cggcgatttc ttccacgatg tcgcgcaaac ggaagaactt tccgctgtcg
     1501 gcctctgccc aggcgccgtc gcccagccag tgcgacgggt gattgcagat ctgtttcagc
     1561 cgcaggagga aggcgagaac gatgcccctg cgctcgattc cctccttgtc ggcgagcgcc
     1621 tcggcaagat cgcccaccgc ctcctggtag agcgccgcct gcctgcggct cagcggacag
     1681 aacaccctga cctcggtctt gtcgggcaga tccgcgatga tgctgcggtc ggtcttcagg
     1741 cggcgcagga tgtagggcga gacgagctcg cgcagcggcc catagggatt gtgcgcccgc
     1801 tcggcgaggc tcttgacgaa agctgaaaat tccctggacg aacccagcag tccgggattg
     1861 aggaaatcga agatcgacca cagatcgccg aggcggttct cgatcggcgt gccggtcagc
     1921 gcgatgcgcg cctgggcgtt gagacgcttg acggatcgtg tctgattggc ggcgggattc
     1981 ttgatcgcct gcgcctcgtc gaggatcgcg agccgccagg gaatctccac catccagggc
     2041 gcgcgggcga gaaagccgta gctcgtgatg accagatcgg cgcctgagaa atctcccgag
     2101 ccgtcgagcc tggggcgcga ttccggcggc gtggagggat gggcgacaat cgccacgacg
     2161 ctcggggcga agcggtcgat ttcgctcgac caattggcga ggagagaggc cggcgcgacg
     2221 agcagggagg gtttgccggg cgcctcgcgc ttcaggacga gcagcaggga aagaatctgg
     2281 attgtcttgc cgagccccat gtcgtcggcg aggcaggcgc caagcccgag ccgcgagaga
     2341 aggtacatcc aatgcgcgcc cgatcgctga tagggcctga gcgtgccgtg aaaggacgga
     2401 ccggggtcga cgagcgcgcg tgttccgccg tgccgcagtc ccgccagcgt ttccgcaagc
     2461 caggggccgg cgacggtctc gctccagtcg atttcggcgt tggccgatat atcgtccggg
     2521 gataagggcg cacgggcgag caggcgcatc gcctcgccga acgacaggcc ttcctcgccg
     2581 gcgcggcgcg cgaccgcctc gaactgttcg atcgtgcggg caagccgcgc ggagtccact
     2641 tcgacccatt tgccgcggat gaacgccagt ccgtccgatt gtgcgagcag aagctcgatc
     2701 tcctccggcg cgagggtctc gccgtcgagc gtcagctcca tgcggaaatc gagcagcgca
     2761 ttcatgccga gctgtgacgg ggcgtttccg ccgacggtgg ctctcatggt cgggcgcgcc
     2821 ggacggttca tgcgccagtt cgggggcatg cgcacgacaa cgccggcgct ctcgagtgca
     2881 ggaacgtctc tcaggaattg cagcgcgtcc tgcgcactcc agcgcagcgg gtgaaaaatc
     2941 tcgccggcgt cgaccatgtg ccgcagccag gcgcaaggcg cggcggcgcg ctgcaccggc
     3001 gcgaggagcg acagcaggcg tgcgcggtcg cccgcatatt cctgcaatgc ccggccgagc
     3061 ggcaggtgct gcgcgcggcc ctgggcggaa aggcgcgtcg tgtaggtggc gaggaacgcg
     3121 aaaggcgcgc tttcgtcctt tcggttctcg gccagactga aatgcacgcg gccgacgagg
     3181 ttccacgccg cattgcgggc cttgagaaaa tcggcgatcg gcagccttgc ctcgctgcgc
     3241 tccacgtcga acgccgcatc gatggcgcgc cacagaccgt cgagtacggc ggcggagagg
     3301 tattcggccc ccagcatggg cggcgccgag agcaccatcg tcgcaaacgc gccgtccgca
     3361 ggcggcggca aggcaggttt ggcggcgcct tcgctcagct cggggagcgc acagagcccg
     3421 gcaaggtatt gcgctccaag ctcccgccaa taggagagaa gcggcgggag cacggtgccg
     3481 acttcgtctg cgccgagaca aagcaatccg tggccgctgc cgcgcgcaaa tgcggcttcg
     3541 agacgcgcgc ccggatcgcc cgcaagctcg ggcgcatcct ccatgcggcg gaggatcaat
     3601 gcgccatgcg gcgtcagaac cggcgcgagc ttcatgcgga gaacgtcatg gccatcatgt
     3661 cggcgacgca tgtttcacat gaaacaatcg gtcgcagttt ggtcctgatt ctcctgtttg
     3721 ccgccaccgc agttcctgtt ctttcaaata actgccgctg cgcaaatcgt tgattgcctt
     3781 gcgcattcgc gtccgggcag cccgatttcg gcgtctggcg caagaatcgc ctgtatttcc
     3841 cctgataaac aggagccaac gccacaaagc gtcattcggc ccgcggcagc gcggcgtaac
     3901 gcgccgcttc gtcggcggcg ctgaacagcg ccctggcctt gttgacgctt tcctcgaatt
     3961 ccaattgcgg cacgctgtcg gcgacgacgc cgccgcccgc ctgcacatac atcatgccgt
     4021 ccttcaccac cgcagtgcga agtacgatgc aggtgtccat cgtgccgttc gccgcgaaat
     4081 agccgaccgc gccggcatag gctgcgcccc gtttttccgt ttcgaactca tcgatgattt
     4141 ccatcgcgcg aatcttgggc gcgccggtca gtgtgccggc cggcattccc gcggacagcg
     4201 cgtcgatggc gt
//
LOCUS       DASZMC010000563         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1628373,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000563 DASZMC010000000
VERSION     DASZMC010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628373"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VG274_11390"
     CDS             <1..233
                     /locus_tag="VG274_11390"
                     /inference="COORDINATES: protein motif:HMM:NF017931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HWE07305.1"
                     /translation="FGGDPAIMGAIGMLSGFCGTLTTPMAANFNIVPAALLELPDRYA
                     VIKAQLPTALLLLAANTILMYVLVFRFGAHHA"
     gene            230..1249
                     /locus_tag="VG274_11395"
     CDS             230..1249
                     /locus_tag="VG274_11395"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HWE07306.1"
                     /translation="MRSLTKDIASRFAQAALCHVGREWPNKLDHVMGGPVDVKTPRAL
                     HPAFFGSFDWHSCVHGTWLLARLYGRFPQLPENDPIRALLDVQLTSQNIAGEIAYLAR
                     AESRGFERPYGWAWALMLAAELARHRSEEGRSWSRAVAPLAQAFERRFEAWLPLATYP
                     VRAGTHANTAFALLLALGYARTVDNSSFTALCTEKSLQWYANDLDCQAWEPCGDDFLS
                     STLVEAACMRKVLGRDDFARWFSRFLPRLAQGEPAPLFSPAFVSDRSDGKIAHLDGLN
                     LSRAWCWRVIVDAIADQDTRGRIEDSIDRHLDSALPHVTGEYMGEHWLASFALLAMDS
                     DPPAA"
     gene            1429..1641
                     /locus_tag="VG274_11400"
     CDS             1429..1641
                     /locus_tag="VG274_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07307.1"
                     /translation="MAANDDAVCRSGGADRGTEAYSGCRSQLTEEREQVSTQYLQNQQ
                     MYEPAPAANMPYHPTADAVTVVELHL"
     gene            complement(1681..>2181)
                     /locus_tag="VG274_11405"
     CDS             complement(1681..>2181)
                     /locus_tag="VG274_11405"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single strand break in
                     duplex DNA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWE07308.1"
                     /translation="RLAEGLMLKRWDSIYQAGRPKGPWFKWKRDPQLVDAVLMYAQRG
                     HGKRSSYYSDYTFGLWREDASGERTLTPVGKAYFGFTDEELKALDKFVRDNTTERFGP
                     VRAVRADRDFGLVLEIAFEGLQRSTRHKSGVAMRFPRISRIRWDKPAREADELATLER
                     MLDARR"
BASE COUNT          386 a          699 c          663 g          433 t
ORIGIN      
        1 aattcggcgg cgacccggca atcatgggcg caatcggcat gctgtccggt ttttgtggaa
       61 cgctgacgac gcccatggcc gccaatttca atattgtgcc cgctgcactc ctcgagctgc
      121 ccgaccgata tgcggtcata aaggcgcagc tgcccaccgc ccttctgctt ttggccgcca
      181 acaccatcct gatgtatgtg ctggtcttcc gttttggcgc gcaccacgca tgagatcgtt
      241 gacgaaggac atcgcttcgc gtttcgcgca ggccgctctc tgccatgtcg gacgcgaatg
      301 gccgaacaaa ctcgaccatg tgatgggtgg ccccgtcgac gtgaagacgc cgcgcgcgct
      361 gcacccggcg tttttcggca gcttcgactg gcattcctgc gtgcacggca cctggcttct
      421 tgcgcggctc tacggccgat ttccgcaact tcctgagaac gacccaatcc gcgcgctatt
      481 ggatgtgcag ctcacatcgc agaacattgc gggcgagatt gcctatctcg cgcgcgccga
      541 aagccgcggt ttcgaacggc cttatggctg ggcatgggcg ctgatgctgg cagccgagct
      601 tgcacgccac cgaagtgaag aaggcaggag ctggagccgc gcagtcgcgc cgctcgcgca
      661 ggcgttcgaa cgcaggttcg aagcctggct tccgcttgcc acctatcccg tgcgcgcggg
      721 cacgcacgcc aacacggcat tcgcgcttct tctcgcgctc ggctacgcgc gcacggtcga
      781 taattcgtcg ttcactgctc tctgcacgga gaagtcactg cagtggtacg caaacgacct
      841 ggattgtcag gcctgggagc cctgcggcga cgactttctt tcctcgacgc tcgtcgaggc
      901 cgcttgcatg cgcaaagttc ttgggcgcga cgatttcgcg cggtggttct cacgctttct
      961 tccacgcctt gcgcagggcg aacccgcgcc gctgttttcg ccggctttcg tcagcgaccg
     1021 cagcgatggc aagatcgcgc atctcgatgg gctcaacctg tcgcgtgcct ggtgctggcg
     1081 ggtaatcgtc gacgcgatcg ctgatcagga cacgcgcgga cgcattgaag attcgattga
     1141 ccggcatctg gacagcgcgc tcccgcatgt gacaggcgaa tatatgggcg aacactggct
     1201 ggcgagcttc gccctgctcg cgatggactc cgatccgccc gcagcgtgac aaagcgtgtg
     1261 ctgaataaaa ggcgcggaaa cccgttagcc tgccgctaac gccgaggctc tatacggaaa
     1321 gcggaatggc gcgacttggg tcgcggccac cgattcgagg gcctggacat gagatcggga
     1381 ctgcttgcat tggcgttgct ccttgtgttg ccgggatgtg cgtcccgcat ggctgcaaat
     1441 gacgatgccg tttgccgttc ggggggcgcg gatcgcggaa cggaagctta ctccggttgc
     1501 cgcagccagc tcaccgaaga acgcgaacag gtgtccacgc aatatctgca aaaccagcag
     1561 atgtatgagc cagcaccggc cgcaaacatg ccttaccacc cgacggcaga tgccgtcacc
     1621 gttgtcgaat tgcatctctg acggcggcgg ttttcgcaaa ctgtgattga tcgcatacgc
     1681 tcaacggcgc gcgtccagca tgcgctcaag cgtcgctagt tcatcggctt cccgcgcggg
     1741 cttgtcccag cggatgcggc tgatgcgcgg gaagcgcatc gcgacgcccg atttgtggcg
     1801 cgtcgagcgc tgcaagccct cgaaggcaat ctcgagcacg aggccgaaat cccggtccgc
     1861 gcgcaccgcg cgcaccggac cgaaccgttc ggtcgtgttg tcgcgcacga atttgtccag
     1921 cgccttcagc tcctcgtcgg tgaaaccgaa ataggccttg ccgaccggcg tcagcgtccg
     1981 ctcgccgctc gcatcctcgc gccagagacc gaaggtgtag tccgaatagt agctcgaacg
     2041 cttgccgtgg ccacgctgcg cgtacatgag cacggcatcg accagttgtg gatcgcgctt
     2101 ccatttgaac cacggtcctt ttggccgccc agcttggtag atcgaatccc agcgctttag
     2161 catcaaacct tcggcgagac g
//
LOCUS       DASZMC010000564         5981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5178115,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000564 DASZMC010000000
VERSION     DASZMC010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5178115"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VG274_11410"
     CDS             <1..562
                     /locus_tag="VG274_11410"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWE07309.1"
                     /translation="TRRNGMKRLMMFASMMAAMFAGPAMAALAVGAAAPDFSAQASLG
                     GKEFSFSLADALKKGPVVLYFYPAAFTKGCTIEAHDFAEATAQFAALGATVIGVSHDS
                     IATLDKFSVSECRSKFAVAADPDETVIKSYDAVLAQKPEYANRTSYVIAPDHTVLMTY
                     TDLNPDKHVELTLAAVKKWRDAQVQK"
     gene            610..966
                     /locus_tag="VG274_11415"
     CDS             610..966
                     /locus_tag="VG274_11415"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWE07310.1"
                     /translation="MIEITQEIVFDAAHSLGTGAPENRRVHGHSFYVDVTLRGEPAAA
                     TGMLREFGEVKSVLATIVDELDHRMLNDVPGLGAPTLENLARFIFGRAKEKLPEVARV
                     RIRRPSCGQSCLYEEA"
     gene            970..1428
                     /gene="queF"
                     /locus_tag="VG274_11420"
     CDS             970..1428
                     /gene="queF"
                     /locus_tag="VG274_11420"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWE07311.1"
                     /translation="MARQTKLTQLGKRVSAPQSPQSAHLETVANPHPDQDYVVRFTAP
                     EFTTLCPITGQPDFAHFIIDYCPARHLVESKSLKLLLAGFRNHAAFHEDCTLFIGKRV
                     VAAIHPKYLRIAGYWYPRGGMPIDVFWQTGKLPRSVWLPDPGVAPYRGRG"
     gene            complement(1432..2034)
                     /locus_tag="VG274_11425"
     CDS             complement(1432..2034)
                     /locus_tag="VG274_11425"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWE07312.1"
                     /translation="MNTGAVRCPWPGKDPLYVAYHDEEWGVPEFDDRALYEKLVLDGF
                     QAGLSWITILRKRENFRRAFAGFEPQKIARFGQRQVSRLLQDEGIIRSHAKIEAAIKG
                     ARLWLEIMEKDKGGFTHFIWKHVDGRPRVNRYRTTGQVPAKTRMSEALSKDLKDRGFN
                     FCGPVIVYAFAQAVGMVNDHIVACDRYEPCAKLAAAAQRR"
     gene            complement(2031..2534)
                     /locus_tag="VG274_11430"
     CDS             complement(2031..2534)
                     /locus_tag="VG274_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE07313.1"
                     /translation="MLHHVSIGVRDVASAAEFYDAVLGALGYKRTAQYLPYAIAYGEG
                     VSEFWIQLPHDRETPSPGNGAHVGFSARSQDAVNRFHKAALASGGTDDGAPGPRPDYG
                     PDYYGAFVRDPFGNKVEATLLIVAKKKAAPRTKARAKPAARRSVKTKAKTKSRRPTAK
                     KRKAKRR"
     gene            complement(2586..3401)
                     /locus_tag="VG274_11435"
     CDS             complement(2586..3401)
                     /locus_tag="VG274_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07314.1"
                     /translation="MSSPFVLTDRAILAVSGADSRNFLQGLITNDVGRLASGAVLYAA
                     LLTPQGKILFDFLLYSDECGVLLDCAHEAAEALHRRLSLYRLRAKVDIARRDDLAVLA
                     RWGGESGSAGWWRDPRLLALGWRTVVERTDVAAAAASASAYLDHRLALGVPEGPDFGQ
                     DRMFALDADLDELHGVSFDKGCYVGQELTARMKHRGTARKKLLPVETSAHSLPEAGTP
                     ITAGGSEIGAITSVYDGRGFALVRTDRLEGAGSAALDARGIPVRVLKPPWLFS"
     gene            complement(3442..4569)
                     /locus_tag="VG274_11440"
     CDS             complement(3442..4569)
                     /locus_tag="VG274_11440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWE07315.1"
                     /translation="MSGLPRASPKQLHLLLAPKERFEPGGAGAFALNVVETSLASRWQ
                     AGITVFGSPVARPFPGIHFYPIEVSRWSLRGRNVAMAGRYIDVVKENPPDVAEIYNRP
                     VMVEPLVRQLAHVPIALHFGNDPRRMDGSRSIRERRLLLGRCAAIICVSDFIRRCFLD
                     GIDGPVGERASVVHTGVEWSPEFPAGKERKILYVGRIVPEKGVLELVQALARVLSTHP
                     QWHAEILGAHWFGTAGAPSEYEQKVSRAAAGCDRILLAGFRPHDQVVSSLRSANIAVV
                     PSLWDDPFPRTVLEALAQGCALICSRNGGIAEIGVERALFLDTISADAIAGALERLIS
                     NDTERLALQRRGWESSPFDVVRTTRQLDDLRGAIMQASPGR"
     gene            complement(4566..5615)
                     /locus_tag="VG274_11445"
     CDS             complement(4566..5615)
                     /locus_tag="VG274_11445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWE07316.1"
                     /translation="MILNCMLGTGYGGLEKLFLDEIEMLPMAGLAARGLVRRESPLAR
                     YARDRGLAFDEMIALSDWDPLSIAGARRIVRRQAPQLIMCVGRKAHRLLGRAIGTRIP
                     IVPMVQKRRFDRDFPYAGVLVAAEHRRRTLIEDGVPAQDIIVIPNAVRLPERPKWNYR
                     TGDTTKIVTLGRLHEKKGFGVLIEAIGILASRSVDCTCAIAGDGPERSNLQSQIDRGT
                     LASRISLSGWTDNVADFLTSGDIFVLPSFQEDLPLAVLDAMASGMPIVASDIDGPNEL
                     LVDGETALLVPSHDPEAVASALTRLAQDEILRERLGRGARAAAKRSYSFGAVGRSLAL
                     ALHNVLEGRPISSGT"
     gene            5680..>5981
                     /locus_tag="VG274_11450"
     CDS             5680..>5981
                     /locus_tag="VG274_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE07317.1"
                     /translation="MFDLLISGGTVVSPMGIGSADVGTRDGRIAAIGSLSGVPAVETY
                     DARGLHVLPGVIDTQVHFREPGNEHKEDLESGSRGAVLGGVTAVFEMPNTHPPTTT"
BASE COUNT         1118 a         1875 c         1886 g         1102 t
ORIGIN      
        1 gacgaggaga aatggcatga agcgactgat gatgttcgcg tccatgatgg ccgcaatgtt
       61 tgccgggcct gcgatggcgg ccctggctgt aggagctgcg gcaccggatt tttcggctca
      121 agcctcgtta ggcggaaagg agttcagctt ctccctcgcc gatgcactca agaagggtcc
      181 ggtcgtcctt tatttctatc ctgcggcgtt caccaagggt tgcacgatcg aggcgcatga
      241 ttttgcggag gcgactgcgc aatttgcggc cctgggggca acggtcatcg gcgtgtcgca
      301 tgattcgatc gcaactctcg acaaattctc ggtcagcgaa tgccgcagca aattcgcggt
      361 tgccgccgat ccggacgaga cggtgatcaa atcctacgac gcggttctgg cgcagaaacc
      421 ggaatacgca aaccgcacct cctatgtgat tgcgcccgac cacacggtcc tcatgaccta
      481 caccgatctc aatccggaca agcatgtcga gctgacgctt gccgcggtca agaaatggcg
      541 cgacgcgcag gtccagaaat agccgtcggt gcttgacggg cgcgacgcgg cctgccaaac
      601 gcgcgcgcca tgatcgagat cacgcaggag atcgtcttcg atgcggcgca ttcccttggc
      661 accggcgcgc cagaaaaccg ccgcgtccat ggtcattcct tttatgtcga tgtgacgctt
      721 cgcggcgagc ctgcggctgc gaccggcatg ttgcgggagt tcggcgaggt caagtcggtg
      781 ctggcgacca tcgtggacga gctcgatcat cgcatgctga atgacgtgcc ggggctcggc
      841 gcgccgacgc tcgagaatct ggcgcgcttc atcttcggaa gagcgaaaga gaagttgccc
      901 gaagtcgctc gcgttcgcat ccgccggccg tcctgcggcc agtcctgcct ctatgaagag
      961 gcgtaaccga tggcgcggca gacgaagctg acgcaactgg gcaagcgggt atcggcgccg
     1021 caatcgccac agtcggcgca tctggaaacg gtcgcgaacc cgcatcccga tcaggattat
     1081 gtcgtccgct tcacggcgcc ggaatttacg acgctttgtc cgatcaccgg ccaaccggat
     1141 ttcgcgcatt tcattatcga ttactgcccc gcacggcatc tcgtcgagtc caagtcgctc
     1201 aaactgttgc tggccggctt tcgcaatcac gccgcattcc atgaggactg cacgctcttc
     1261 atcggcaagc gcgtcgtcgc ggcgatccat cctaagtatc tgcgcatcgc aggatactgg
     1321 tatccgcgcg gcggcatgcc catcgatgtg ttctggcaga cgggaaaact tcctcgtagc
     1381 gtctggctgc ccgatcccgg tgtcgcgcct tatcgcggaa ggggatgatc actatctgcg
     1441 ctgcgccgcc gcggccaatt tcgcgcaggg ttcgtagcgg tcgcaggcga cgatgtggtc
     1501 gttgaccatg ccgaccgcct gcgcaaacgc ataaacgatc accggtccgc aaaaattgaa
     1561 cccgcggtcc ttcagatctt tcgacagcgc ctcgctcatt ctggtcttcg ccgggacctg
     1621 gccggtcgtt ctgtagcggt tgacgcgcgg ccgtccgtct acgtgcttcc agataaaatg
     1681 cgtgaagccg cccttgtcct tctccatgat ctccagccag aggcgtgcgc ctttgatcgc
     1741 cgcctcgatt ttggcgtggc tgcgaatgat gccctcgtct tgtagcaggc ggctgacctg
     1801 tctttggcca aagcgtgcaa tcttttgtgg ttcgaaaccg gcgaatgccc gccggaaatt
     1861 ctcccgcttg cgcaatatgg tgatccacga cagaccggcc tggaatccgt cgagcacgag
     1921 cttctcatac agcgcgcggt cgtcgaattc cggaacgccc cattcctcat cgtgataggc
     1981 gacgtaaagc ggatccttgc cgggccacgg gcagcggacc gcgccagtgt tcaccggcgc
     2041 ttggctttgc gctttttcgc ggtggggcgc cgggatttcg ttttggcctt ggttttcacg
     2101 ctgcggcgcg cagctggttt ggcgcgggcc ttcgtacgcg gcgccgcctt ctttttcgcc
     2161 acgataagca gcgttgcctc gaccttgttc ccgaacggat cgcgcacgaa ggcgccgtaa
     2221 tagtcgggac cgtaatcggg acgtgggccg ggcgccccat cgtcggttcc gccactggcg
     2281 agagcagcct tgtggaaccg gttcacagcg tcctggctcc gcgcggaaaa tccgacatgc
     2341 gcgccattgc ccggactcgg cgtttcgcgg tcgtggggca gctggatcca gaattccgag
     2401 acgccttcgc cataggcgat cgcgtagggc aggtattgcg cagtgcgttt gtagccgagc
     2461 gcgccgagaa cggcgtcgta aaactccgcc gcgcttgcaa cgtcgcgcac gccgatggaa
     2521 acatgatgaa gcatgcgtgc agtctccttg aaggacggcg cactcttacc gtttcgtccg
     2581 tcgcgtcaag aaaaaagcca tggcggtttc aggacgcgga caggaatgcc cctggcgtcc
     2641 agcgcagcgc ttccagcacc ttccagacga tcggtgcgga caagcgcaaa tccccttccg
     2701 tcatagaccg acgttatcgc cccgatctcg ctgccgccgg cagtgatggg cgttccagct
     2761 tcgggcaggg aatgagcgct ggtttcgacc ggcagcagct tcttgcgcgc agtcccgcga
     2821 tgcttcatcc gtgccgtcaa ttcttgcccc acatagcagc ccttgtcgaa ggacacgccg
     2881 tgcagctcgt ccaggtcggc atcgagcgcg aacatcctgt cctgtccgaa atccgggccc
     2941 tcgggtacgc cgagggccag acgatggtcg agataggccg atgcagacgc cgccgcagcg
     3001 gcaacatccg ttctttccac gaccgtgcgc cagccgagcg cgaggagccg cggatcgcgc
     3061 caccagccag cggagccact ttctccgccc catcgcgcca gcaccgcgag atcgtcgcgg
     3121 cgcgcgatgt cgaccttcgc ccgcagccgg tagagcgaga gacgcctgtg cagcgcctcc
     3181 gcagcctcat gcgcgcagtc gagcagcact ccgcattcat ccgaatagag gagaaaatcg
     3241 aaaaggattt tgccttgggg cgtgaggagc gcggcataaa gcacggcgcc ggacgcgagc
     3301 ctgcccacgt cattggtgat caggccttgc aggaaattcc gcgaatccgc tccagagacg
     3361 gcaaggatcg cgcgatccgt cagaacaaag ggggagctca tggctcctga ggtagtcggt
     3421 cgccatgcgg ttgccaagcc gctatcggcc gggcgaagcc tgcatgatgg cgccacgcag
     3481 atcgtcgagc tggcgcgtcg tgcggacgac gtcgaagggc gaggattccc atccacggcg
     3541 ctgcagagca aggcgttcgg tgtcgttcga gatcaggcgc tcgagcgcgc cggcgatcgc
     3601 atctgcggat atcgtatcaa ggaaaagcgc ccgttcgacg ccgatctcgg cgatgccgcc
     3661 attgcggctg caaatgagcg cgcagccctg tgcaagagct tcgagaacgg tgcgggggaa
     3721 gggatcgtcc caaagcgagg ggacgaccgc gatgttcgcg ctgcgcagcg acgagaccac
     3781 ctgatcgtgc ggcctgaaac cagcgagaag gatgcgatcg catcctgctg ccgcgcgcga
     3841 caccttctgt tcgtattcgc taggcgctcc ggcagtcccg aaccagtgtg cgccgagaat
     3901 ctccgcgtgc cactggggat gggtggacaa cacgcgagca agcgcctgga cgagctcgag
     3961 cacgcctttt tcgggaacga tacggcccac atagagtatc ttccgttcct ttccggccgg
     4021 aaactccgga gaccattcga cgccagtatg gactacgctt gccctctcgc caacagggcc
     4081 gtcgattccg tcgagaaagc agcgccggat aaaatcgctc acgcagatga tcgcggcaca
     4141 tcggccaagc agcaaacgcc gctcgcggat cgaacgcgat ccatccatcc tgcgcgggtc
     4201 gttgccgaaa tgcagagcga tgggaacgtg cgccagctgc cgcacgagcg gttcgaccat
     4261 gacggggcgg ttgtagattt ccgccacgtc gggcggattt tctttcacca cgtcgatgta
     4321 gcgccctgcc atcgcgacgt tgcgaccgcg cagcgaccat cttgatacct caatgggata
     4381 aaaatggatt cccggaaagg gtcgggcgac aggcgaaccg aatacggtga tgcccgcctg
     4441 ccaccggctg gcaagcgatg tctccactac gttcaacgcg aaggcgccgg cgccgcccgg
     4501 ttcgaagcgc tccttgggcg cgaggagaag atgcagctgc ttcggactcg cgcggggcag
     4561 cccgctcatg tgccgctcga aatcggccgg ccctcgagca cattgtggag ggcaagtgcg
     4621 aggctgcggc caacggctcc gaagctgtag cttcgcttgg ccgcagcgcg tgcgccacgc
     4681 ccgagccttt cgcgcaggat ctcatcctgg gcaagccgcg tgagtgcgct tgccactgcc
     4741 tccggatcat gggacggaac gagcagggct gtttcgccat ccaccaggag ttcattcggt
     4801 ccgtcgatgt cgctcgccac gatcggcatt ccgctcgcca tcgcatccag caccgcgagc
     4861 ggcaaatcct cctggaatga cggcagcacg aatatgtcgc cgctggtgag gaagtcggcg
     4921 acgttgtctg tccagccgga aagggatatc cgggacgcaa gagtcccgcg atctatctgg
     4981 ctctggagat tgctgcgttc ggggccatcg cctgcaatcg cgcaggtgca atcgacagag
     5041 cggctggcaa gaatcccgat cgcctcaatg aggacaccaa agcccttctt ctcgtgcaaa
     5101 cgaccgagcg tcacgatttt cgtcgtatcg ccagtgcgat agttccattt gggacgctca
     5161 ggcagtcgca cggcgttcgg gatgacgatg atgtcttgcg cgggaacgcc gtcctcgatg
     5221 agcgtgcggc ggcggtgttc ggccgcgacc agcacgccgg catagggaaa atcgcgatcg
     5281 aagcggcgct tctgtaccat gggaacgatc ggaatgcgcg ttccgatcgc gcgtccgagc
     5341 agacggtgcg ccttgcggcc gacgcacatg atgagctgcg gcgcctgtcg gcgtacaatt
     5401 cttcgcgcgc ctgcaatgct gagcggatcc caatcgctca gtgcaatcat ctcatcgaat
     5461 gccaggccgc ggtcgcgcgc gtaacgcgcg agcgggctct cgcggcggac caggccacgc
     5521 gccgctaggc cggccatcgg aagcatctcg atctcgtcga ggaaaagctt ttcgaggccg
     5581 ccataaccgg taccgagcat gcagttgaga atcatgaccg gagctgggct aaggtggcgg
     5641 gtggaagcgg aaataacacg acatccggcg gcggcccaaa tgtttgatct cctcatctcc
     5701 ggcggaacgg tcgtcagccc gatggggatc ggcagcgccg atgtcggaac gcgcgacggc
     5761 cgaatcgcgg caatcggcag cctttcgggc gtgccggccg tcgaaaccta cgacgcgaga
     5821 gggctccacg tcctgccggg cgttatcgat acgcaggtgc atttccgcga gcccggaaac
     5881 gagcacaagg aggatctgga gagcggcagc cggggcgcgg tgctgggcgg ggtaacggcg
     5941 gttttcgaga tgcccaatac gcatccgcct accaccacca g
//
LOCUS       DASZMC010000565         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1048875,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000565 DASZMC010000000
VERSION     DASZMC010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048875"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VG274_11455"
     CDS             complement(<1..699)
                     /locus_tag="VG274_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07318.1"
                     /translation="MRIRRMHRRFYDAGCHGIHPDAIPGIFDGERAGHGVQPALRQCG
                     QARAGAGDRLADHGGGDLHDMTALPGQHVRNRQLRDVEKTIQVDMENISIVVRCERRE
                     GLADENTGIVHERIDPSEMPDGSFYDSFGDGALANIAGHRNHTGIAGFLDEAGICDDG
                     VSGVPVTFDDSGSDALRGTCDDRNFLRLSHGQFPLIGWLGAVRQSRTQRRAPIPLRMN
                     ARRSRLMMAAWVVAM"
     gene            884..1210
                     /locus_tag="VG274_11460"
     CDS             884..1210
                     /locus_tag="VG274_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HWE07319.1"
                     /translation="MIYELRLYRPMPGKLPALLRRFEDHTLKIWERIGIRSVGFWTTL
                     IGESNQQLTYMLAWENMAERERLWSVFAADVVWNEVVAESEREGPIVQNIANQLLRPT
                     AFSPMK"
     gene            1541..3229
                     /locus_tag="VG274_11465"
     CDS             1541..3229
                     /locus_tag="VG274_11465"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWE07320.1"
                     /translation="MADIPAVEGARPIENPPLRVLAAIVLTVFCAVLAGVAVSWIRGA
                     LLPVWLGTAVVVYWLLHRPSREWPVIAVVCWAANVAAAIAIGRGMPRCILLSTVNLIQ
                     ALLVAAPLRIFRLDRDFARPRALCVFYALVLGPATMLSALIGAAFVHRLTGAPFLQVW
                     TEWYITTALGQAVLLPPLMVVRRESLAAIFTRELLPGTLLVLGIVAVTIAINVRFPDY
                     PLGFLFFPAVVLVTFQRGFAGGALALVLVVLYKAGVQHDVHSTVVASDARLRLVFVQL
                     YTAAIGFTVVIVGAGLDQRRKLESSLAAAREEAVAARHAAEAASRAKTMFLANMSHEL
                     RTPLNAVIGFSNLIHREICGPLGNGRYREYASNIQDAGRHLLGIIGGILDMSKIEAGA
                     YEILCEELELNELVGECVELMRERAAERQVSLVPELAGPEWIVADRGAMRQIVLNLLS
                     NAIKFTPAGGNVSVATKTMGDKFVLAVADTGIGIPAEDLHRLGKPFVQLRQATAAGSA
                     QSGTGLGLALVRALAEKHGGTFIIESEAGRGTCAKIEIPILAPADPHACLKSAA"
     gene            complement(3407..4711)
                     /gene="purB"
                     /locus_tag="VG274_11470"
     CDS             complement(3407..4711)
                     /gene="purB"
                     /locus_tag="VG274_11470"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWE07321.1"
                     /translation="MIPRYARPEMTAIWSPETRFRIWFEIEAHAADAMARAGIIPKEA
                     ARAIRDKGATVEFDVARIDAIEREVKHDVIAFLTHLAELIGPEARFVHQGMTSSDVLD
                     TCLSVQLRRASDILIVNVDRLLAALKRRAFEFKHAVTIGRSHGIHAEPTTFGLKLAGF
                     YAEFARARARLAAAREEISVCAISGAVGTFANVDPRVEEHVAHALGLKVEPISTQVIP
                     RDRHAAFFAALGVVASSIERLATEIRHLQRTEVLEAEEYFSAGQKGSSAMPHKRNPVL
                     SENLTGLARMVRGYVAPALEDVALWHERDISHSSVERFIGPDATITLDFALARAAEVV
                     EKLVVYPERMKENLERTKGLVHSQRVMLALTQGGMGREEAYRLVQKHAMQAWAGEQPL
                     LDLLKADKEVSRVLDATTLERLFDLDYHLKHVDTIFARVFGT"
     gene            complement(4830..5294)
                     /locus_tag="VG274_11475"
     CDS             complement(4830..5294)
                     /locus_tag="VG274_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWE07322.1"
                     /translation="MKKTAASAENVYVDPSGLLKLYLHEPESRAMTRWRARLHGAVAV
                     TLFGRVELVNAIGLALARGFLGKTAHKAALAALDDDFVNGRCVVVDVSWRAAFRLADD
                     ISRKWTPRLICRSLDVLHVASALALNRKFFVTFDVRQRELARATGLKLVFPR"
BASE COUNT         1000 a         1673 c         1694 g          969 t
ORIGIN      
        1 catggccacc acccacgcgg ccatcatcaa tcgcgacctc ctcgcgttca ttcgcaacgg
       61 gatcggtgcc cgacgctgag tccgggattg gcgcaccgcg cccaaccaac cgatcaacgg
      121 aaattggcca tgactcaagc gcagaaagtt gcgatcgtca caggtgcctc gcaaggcatc
      181 ggagccggaa tcgtcaaagg ttaccgggac gccggatacg ccgtcgtcgc aaattcccgc
      241 ttcatccaga aatccggcga tcccggtgtg attgcggtgc ccggcgatat tggcgagcgc
      301 accgtctccg aagctgtcgt aaaaactgcc atcgggcatt tcggacggat cgatacgctc
      361 gtgaacaatg ccggtatttt catcggcaag cccttcgcgg cgttcacaac ggacgactat
      421 cgaaatgttc tccatgtcaa cctggatggt tttttccaca tcacgcagct ggcgattgcg
      481 cacatgctga cccggaagag cggtcatatc gtgcaaatca ccaccgccat ggtccgccag
      541 ccgatcgccg gcgccggcgc gagcctggcc gcactgacga agggcgggct ggacgccgtg
      601 acccgcgcgc tcgccatcga atatgccagg gatggcgtcc gggtgaatgc cgtggcaccc
      661 ggcatcataa aaacgccgat gcatgcgccg gatacgcatg aatttctctc ggcactacat
      721 ccgcttggcc ggatgggaga ggtgcatgag atcgtcgacg ccatcctcta tctggaaaag
      781 gccggcttcg taacgggcgc ctccatgtcg atggcggcca acacgccggt cgctggtagg
      841 cagccgggag tcagatccac gatcaagata ttgggagacg ccgatgatct acgaattgcg
      901 cctttatcgc ccgatgccgg ggaaactacc ggcgttgctc cggcgcttcg aggaccacac
      961 gctgaagatt tgggaaagga taggcatccg ctccgtcggc ttctggacga cgctgatcgg
     1021 agaaagcaac cagcagctca cctacatgct cgcctgggag aacatggccg agcgcgagag
     1081 attgtggagc gttttcgccg ccgatgtggt gtggaacgag gtcgtggcag agtccgaacg
     1141 ggagggaccg atcgtgcaga atatcgccaa ccagctgctg cggccgacgg cattttcgcc
     1201 gatgaaataa ggcgaccgcg aaaagttcaa caccaaacgc agcgacagcc tcatcgcgtt
     1261 tgcctggcgg agtaataacc ccaaacgtgg cgcgcggaga cgcggccctc atgattcgac
     1321 agactcacca tgaggcttga gagagagcct caggccgagg agcgttctct cgcctgcccg
     1381 gcgaacgcgg taacctcagg cggcgatggc ggcgatgatc ggacccgggg ccgcggcagg
     1441 aacacactta atgcattctt atggcgccgc agggtaagga acagcaggga aaagtcttcc
     1501 ggacgaaccc gggggccgaa aacgatattg gcgaaggatg atggccgata ttccagccgt
     1561 tgaaggtgcg cgtccaattg aaaacccgcc gctgcgcgtg cttgccgcga ttgtgctcac
     1621 cgtattctgc gccgtgcttg ccggcgtcgc tgtgagctgg atccgcggtg cgctgttgcc
     1681 cgtgtggctg gggacggcgg tggtggtgta ctggcttttg cacaggccga gccgcgaatg
     1741 gcccgtgatt gctgtggtgt gctgggccgc caacgtagcc gcggcgatcg ccatcgggcg
     1801 cgggatgccg cgctgcatac tgctttcgac ggtcaatctc atccaggcgc tcctggtcgc
     1861 ggcgccgctg cgcatcttca ggctcgaccg cgatttcgcc cggccgcgcg cgctctgcgt
     1921 gttctacgcg ctcgttcttg ggccggcgac catgctgtcg gcgctcatcg gcgccgcctt
     1981 cgtgcacagg ctgacgggcg cgccttttct ccaggtctgg acggaatggt acataacgac
     2041 ggcgcttggt caggcggttc ttctgccgcc gctgatggtg gtgcgccggg aatcgctcgc
     2101 ggcgattttc acccgcgaat tgctgccggg cacactcctg gtgcttggca tcgtggccgt
     2161 cacgatcgca atcaatgtcc ggttcccgga ctacccgctc ggcttccttt tctttccggc
     2221 agtggtgctg gtcacgttcc agcgcggctt cgcaggaggc gctctcgcac tcgttctcgt
     2281 cgtactctac aaggccggcg ttcagcacga cgttcacagc acggtcgtgg cgtcggatgc
     2341 gcgcctgcga cttgtcttcg tccagcttta cacggcagcg atcggcttca cggtcgtcat
     2401 cgtcggcgcc ggtctcgatc agcgaaggaa gctcgaatcg agtctcgccg cggcgcgcga
     2461 ggaggccgtc gccgcacgcc atgcggccga agcggcaagc cgcgccaaga ccatgttcct
     2521 ggccaatatg agtcacgaac tgcggacgcc gctgaatgcc gtgatcgggt tttccaatct
     2581 catccatcgc gagatctgcg gtccgctcgg aaacgggcgc tatcgtgaat atgccagcaa
     2641 catccaggat gccgggcgtc atctgctcgg aatcatcggc ggcattctgg acatgtcgaa
     2701 aatcgaggcg ggcgcatacg aaatcctgtg cgaagagctc gagctgaacg agcttgtggg
     2761 cgaatgcgtc gaactgatgc gtgaacgcgc cgccgaacgg caggtgagcc tcgtgccgga
     2821 actggcgggg cccgaatgga tcgtcgccga tcgcggcgcc atgaggcaga tcgtgctcaa
     2881 tctgctgtcc aacgccatca aattcacgcc cgccggcggc aatgtatcgg tggccacaaa
     2941 aacgatgggt gacaaattcg tcctcgctgt ggccgatacc ggcatcggca tccccgccga
     3001 ggatctgcac aggcttggaa aacctttcgt gcagctccgg caggcgaccg ccgcaggttc
     3061 agcgcagtcc ggcacggggc ttggactggc gctggtgcgc gcgctcgccg aaaaacacgg
     3121 cggcacgttc atcatcgaga gcgaagccgg ccggggaact tgcgcgaaaa tcgaaatccc
     3181 gatccttgcg cccgccgatc cgcacgcctg cctgaaaagc gccgcgtgac ggcaaaagtt
     3241 gccgcgcgca gccgcggaga gcgcagagga agaagaaaat ttcacaataa gacagtgaga
     3301 tagtgagaac tatagtgcgc cggaggcgcg cgattccttt ctcaccgtct cactgtctca
     3361 ttgtgcatcc ttttctttct gcgcgtctcc gcgcgaaact ctttgtttat gtgccaaaca
     3421 cgcgggcgaa gatcgtgtcg acgtgtttga ggtgatagtc gaggtcgaac agccgttcga
     3481 gcgtcgtcgc atctaggacg cgcgagactt ccttgtccgc cttgagcaga tcgagcagtg
     3541 gttgctcgcc ggcccaggcc tgcatcgcgt gtttctgcac caggcgatag gcctcctcgc
     3601 gccccatgcc cccttgcgtc agcgccagca tcacgcgctg gctgtgcacc aaaccttttg
     3661 tgcgttcgag attctccttc atccgctccg gatagacgac gagcttctcg accacctccg
     3721 ccgcgcgcgc cagcgcgaag tcgagcgtga tggtggcgtc ggggccgatg aagcgctcga
     3781 ccgaggaatg cgaaatgtcg cgctcgtgcc acagcgccac gtcttcgagc gccggggcga
     3841 cgtagccgcg caccatgcgg gcgaggccgg tgagattctc cgacagcacc ggattgcgct
     3901 tgtgcggcat cgccgacgag cccttctggc ctgccgagaa atactcctcc gcctccagaa
     3961 cttcggtgcg ttggagatgg cggatctcgg tcgccagccg ctcgatcgaa ctggcgacga
     4021 cgccgagcgc ggcgaagaac gccgcgtggc ggtcgcgcgg aatcacctgc gtcgaaatgg
     4081 gctcgacctt cagccccagc gcgtgggcga catgttcctc gacgcgcggg tcgacattgg
     4141 cgaaggtgcc caccgcgccc gatatcgcgc acaccgagat ctcctcgcgc gcggcggcga
     4201 ggcgggcgcg ggcgcgggcg aactccgcgt aaaatcccgc gagcttcagc ccgaaagtgg
     4261 tgggctcggc gtgaatgccg tggctcctgc cgatcgtgac cgcgtgtttg aactcgaagg
     4321 cgcggcgctt gagcgcggca agcagcctgt ccacattcac gatcaggatg tcgctggcgc
     4381 ggcgcagctg gaccgaaagg caggtgtcga gcacgtcgga cgaggtcatg ccctgatgca
     4441 cgaagcgcgc ctcggggccg atgagttcgg ccagatgggt gaggaacgca atcacgtcgt
     4501 gcttgacctc gcgctcgatc gcatcgatgc gcgccacgtc gaactcgacc gtcgcgccct
     4561 tgtcgcggat cgcgcgcgcg gcttccttcg ggatgatgcc ggctcgcgcc atcgcatcgg
     4621 ccgcatgcgc ctcgatctcg aaccagatgc ggaacctggt ctcgggcgac cagatcgcgg
     4681 tcatttcagg ccgggcgtag cgggggatca tggagcggtc acggcgtacc tgggcaagag
     4741 cagaacgacc ctaacttgag cggagcacag acgaaagccc gcaccgcaac gcgcgcttga
     4801 tggccttgac cgcagcgaat gcgtcactgt catcgcggga aaacaagctt cagtcccgtc
     4861 gcgcgcgcga gttcgcgctg gcgaacatcg aacgtcacga aaaatttgcg gttcagcgcc
     4921 aaagcgcttg caacatgcaa aacgtccagc gagcggcaaa tcagacgcgg agtccatttg
     4981 cgactgatgt cgtccgcaag tctgaacgcc gcgcgccagg atacatcgac gaccacgcat
     5041 cggccgttca cgaaatcgtc gtccagcgcg gccagcgctg ccttgtgggc ggttttgcca
     5101 agaaacccgc gagccaatgc cagaccgatc gcgttgacga gctccacgcg gccgaagagc
     5161 gttacggcaa ccgcgccatg gagccttgca cgccaacggg tcatcgcccg ggactcgggc
     5221 tcgtgcagat agagcttcag aagcccgctc ggatcgacgt agacgttctc agcgctcgcc
     5281 gcggttttct tcatgaagct tgcgcgattg cgctttcgtc agcggccgcg gctgat
//
LOCUS       DASZMC010000566         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_799169,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000566 DASZMC010000000
VERSION     DASZMC010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799169"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VG274_11480"
     CDS             <1..1248
                     /locus_tag="VG274_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07323.1"
                     /translation="PGRYKTEEKLSSESEWARLTRAFSHIANEPGFALVAPSEILRGA
                     DLKRQPLRLESAACPIPVKKQRKYNLSRWAVTGRDDLGINAACERIYHGLEAANADRV
                     SWKELCYLWSSDFRTHITDKRWIAFCARLQDTETRWPGTEPATPLPQGAAITQRHIDI
                     TTPALTARLDRRRGLALSSLHFAHHTKAALGGLPHGHFDDIQVQADWYTGDSVFEAPG
                     EHKITDLENCEARSWVANGDTFAFAKIDTPKGPIEKTLRFHANAPRVDFDLAFHWQEF
                     GKGSLRLGHFTLLPDAFDWSKLSLTTHNGGKTSETFALADQTVDHGAPVSFLVSASHG
                     LGMTEGWAEIGDDKTRLRIEIDRTAAPLLGLLTHRRIGGSLFCQFVLSALELDDTRKP
                     TLYRDGPRRFRFSVNGVLPVAAC"
     gene            1307..2392
                     /gene="pseB"
                     /locus_tag="VG274_11485"
     CDS             1307..2392
                     /gene="pseB"
                     /locus_tag="VG274_11485"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HWE07324.1"
                     /translation="MKKIYLAVKDDHETAPPANTSLRDFTQPWLDLNDKSVLVTGGTG
                     SFGKHFVKTVIENYKPRRLVIYSRDELKQYEMQQMFPMEQHPFMRYFIGDVRDRDRLD
                     MALNNIDYVIHAAALKQVTTAEYNPFECIRTNVFGAENLVYAALRRNVRKVIALSTDK
                     AANPVNLYGASKLASDKIFVAANNLAGADGTKFSVVRYGNVFGSRGSVVPLFRKLAAE
                     GAETLPITDPRMTRFWITLTQGVNFVLSSMELSRGGEIFVPKIPSTTMTDLATTICPS
                     LGHKIVGVRPGEKLHETMIPADDSRWTVEMEDRYIILASFAAAAREAYLNRGAKPVTE
                     GFAYSSDCNPETLDVRGLQSLLAEAFA"
     gene            2389..>2810
                     /locus_tag="VG274_11490"
     CDS             2389..>2810
                     /locus_tag="VG274_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWE07325.1"
                     /translation="MSLPALKVARAAEAPTQSFLPYGRQVIEDDDIAAVTEVLRGDYL
                     TTGPAVARFEEAMAKYVGAKHAVVCANGTAALHMAARALELGQGSKIIVPAITFLATA
                     SAPHMNGAEIVFADVDPTTGLMTPETFSNALARAGRADA"
BASE COUNT          564 a          888 c          811 g          547 t
ORIGIN      
        1 cccggccgct acaagacgga agagaagctt tccagcgaga gcgaatgggc gcgactgaca
       61 cgcgcgtttt cgcatatcgc aaatgagccg ggcttcgcac tggttgcacc gtccgagatt
      121 cttcgtggtg cggacttgaa acgccaaccg ttgcgcctcg aaagcgcggc ctgccccatc
      181 ccggtcaaaa agcagcgtaa atacaatctc tcgcgctggg ccgtcaccgg ccgagacgat
      241 ctcgggatca acgccgcctg cgaacgcatc tatcacggcc tcgaggcagc gaacgccgat
      301 cgcgtatctt ggaaagagct ctgctatctg tggtccagcg atttccgtac gcatatcacg
      361 gataagcgtt ggatcgcgtt ctgcgcacgt ctgcaagaca cggagacgcg ctggcctgga
      421 actgaacccg cgacgccgct tccgcaaggt gctgcgatca cgcagcggca tatcgacata
      481 acgacaccgg cacttaccgc acgcctcgac cgccgccgcg ggctcgcgct gtcatcgttg
      541 catttcgcac atcatacaaa agcggcgctt ggcggattgc cgcacgggca tttcgacgac
      601 atccaggtac aggcggactg gtacaccggc gacagcgttt tcgaggcgcc gggtgagcac
      661 aagatcaccg atctcgagaa ctgcgaggcc cgcagctggg tcgcgaacgg cgacactttt
      721 gctttcgcaa aaatcgatac gccgaaaggc ccgatcgaga aaacgttgcg ctttcacgca
      781 aacgcaccgc gtgtggattt cgatctcgcg tttcattggc aggaattcgg caagggcagt
      841 cttcgcctcg gccatttcac gctgctgccc gatgcattcg actggtctaa gctttcctta
      901 acgacgcaca atggcggtaa gacttccgaa actttcgcgc tcgcagacca gaccgtcgat
      961 catggcgcgc cggtttcctt ccttgtttcg gcatcgcacg ggctcggcat gaccgaaggc
     1021 tgggccgaga tcggcgacga caaaacccgc ttgcgcattg agattgaccg cacagcggcg
     1081 ccacttctgg ggctcctgac ccatcgccgt atcgggggca gcttgttttg ccagttcgtc
     1141 ctgtcggctc ttgaactcga cgacacgcgc aagcccacgc tttatcgtga cggcccccgg
     1201 cgttttcgat ttagcgtgaa tggagtgttg ccggtagccg cttgttaact gtgttctgca
     1261 accatgtctt cgcacaaagc ggacgagaga catggggcag caccgggtga aaaagatcta
     1321 tctcgcggtc aaggacgatc atgaaaccgc gccgccggca aataccagcc ttcgcgattt
     1381 cacgcagccc tggctcgatc tgaacgacaa gtcggtgctc gtcacaggcg gcacaggctc
     1441 gttcggcaag catttcgtca aaaccgtgat cgagaactac aagccgcgcc gtctggtgat
     1501 ttattcgcgc gacgaactca agcaatacga gatgcagcag atgttcccca tggaacagca
     1561 tccgttcatg cgctatttca tcggcgatgt gcgcgatcgc gaccgcctcg atatggcgct
     1621 caacaacatc gattacgtga ttcacgcggc ggcgctgaag caggtgacga ccgcagaata
     1681 caatccgttc gagtgcattc gcacaaatgt gttcggtgcc gaaaatctcg tctatgccgc
     1741 cctgcgccgc aatgtgcgta aagtgatcgc gctgtcgacg gacaaagccg cgaacccggt
     1801 caacctctat ggcgcgtcca agcttgcctc cgacaagatc ttcgtcgccg ccaataatct
     1861 ggccggtgcc gacgggacca agttctccgt cgtgcgttac ggcaacgtgt tcggctcgcg
     1921 tggcagcgtc gtacccctgt tccgcaagct tgcggctgag ggcgccgaga cgcttccgat
     1981 caccgatccg cgcatgacac gcttctggat cacgcttacg caaggcgtga acttcgtgct
     2041 gtccagcatg gaattgtcgc gcggcggcga gatattcgtg ccgaaaattc cctcgaccac
     2101 gatgaccgat ctggcaacca cgatctgccc gtcgctcggc cacaagatcg tcggcgtgcg
     2161 tcccggcgag aagctgcacg agaccatgat cccggccgac gactcccgct ggaccgtcga
     2221 gatggaggat cgctacatca ttctggcaag ctttgccgcc gcggcgcgcg aggcctatct
     2281 caaccgcggt gccaagcccg tgaccgaggg ctttgcctat tccagcgact gcaatcccga
     2341 aacactcgac gtgcgcggct tgcaatctct cctcgccgag gccttcgcgt gagtctgccg
     2401 gcgctgaaag tcgcgcgcgc ggcggaagcg cccacgcaat cgtttcttcc ctatggccgg
     2461 caggtcatag aagatgacga cattgccgcg gtcactgagg tgctgcgcgg cgattatctc
     2521 accaccggtc ccgccgtcgc ccgcttcgag gaagcgatgg cgaaatatgt cggcgccaaa
     2581 catgctgtgg tctgtgccaa cggcacagcc gcgcttcata tggcggcacg tgcgctcgag
     2641 ctcggtcagg gttcgaagat catcgtgccg gcgatcacgt ttcttgcgac cgccagcgcg
     2701 cctcatatga acggcgctga aatcgtcttc gccgatgtcg atcccacgac cggcctgatg
     2761 acacccgaga ctttcagcaa tgctctggcg cgcgcaggcc gggcggatgc
//
LOCUS       DASZMC010000567         4384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2979190,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000567 DASZMC010000000
VERSION     DASZMC010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2979190"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VG274_11495"
     CDS             <1..355
                     /locus_tag="VG274_11495"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HWE07326.1"
                     /translation="YRRGIRHIVGGMCETDYSGYPDCRDETIRALQTALNLGMDTQFE
                     LHTPLMWLDKAATWRLADELGGAGLVGLICEHSHTCYLGERGARHDWGFGCGECPACK
                     LRAKGWWEYAGAKLA"
     gene            complement(428..808)
                     /locus_tag="VG274_11500"
     CDS             complement(428..808)
                     /locus_tag="VG274_11500"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HWE07327.1"
                     /translation="MDAATAREATSAVPGIPRDDDGPVFREPWEAQAFAMALALHERG
                     LFSWNEWAAALADEIKRAQASGDPDTGETYYRHWLATLERLVAEKGVASSKTLHLYRD
                     AWDHAADRTPHGSPIDLRAEDFAN"
     gene            complement(808..1467)
                     /gene="nthB"
                     /locus_tag="VG274_11505"
     CDS             complement(808..1467)
                     /gene="nthB"
                     /locus_tag="VG274_11505"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HWE07328.1"
                     /translation="MNGVHDMGGMDGFGKVEPEPNEPMFHAEWEARVLAMVRAMGAAG
                     AFNIDASRFYREKLPPDVYLASSYYKKWLLGLEDLLIDKGFIAAGEIAAGHGTEPAKP
                     LKRGKFTLDDIERIMVRGKFARAAPAPAKFKPGDHVRTKNIHPRTHTRLPRYVRGHVG
                     VVERDHGCQVFPDSAAAGSGENPQWLYTIVFDGTELWGPDADPTVKISIDAFEPYLET
                     A"
     gene            complement(1464..2096)
                     /gene="nthA"
                     /locus_tag="VG274_11510"
     CDS             complement(1464..2096)
                     /gene="nthA"
                     /locus_tag="VG274_11510"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HWE07329.1"
                     /translation="MSDRDHDHHHDHDHSDLSETELRVRALETVLTEKGYVEPAALDA
                     IIQAYETKIGPHNGARVVAKAWSDPEFKKALLDDGTRAVSTLGHVSRVGDHLVVVENT
                     WQRHNMVVCTLCSCYPWEMLGLPPVWYKAAPYRSRAVKDPRGVLADFGVSLPKETEIR
                     VWDSTAETRFLVLPMRPEGTGGWNEQQLAELVTRDSMIGTGFPKAPGAPS"
     gene            2259..2750
                     /locus_tag="VG274_11515"
     CDS             2259..2750
                     /locus_tag="VG274_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWE07330.1"
                     /translation="MQLENKKIAILATHGFEQSELEVPRDKLKAAGAIVHIVSLQAGE
                     IRGWDMKDWGRPVKVDKTLDQVSADDYDAIVLPGGQINPDLLRVEPKALAFIKDIFNQ
                     KKIVAAVCHAPWLLIETGIAKGRRMTSYKSIKTDVKNAGAKWEDSELWSTPALLRRAI
                     PAT"
     gene            2912..3706
                     /locus_tag="VG274_11520"
     CDS             2912..3706
                     /locus_tag="VG274_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWE07331.1"
                     /translation="MTIEKGLGPEGRIAVVRFDRGDGVNALSPEALRQLTGAARSFED
                     DGDTSVVVLTGGNNAFSAGFDLKDPEGRSRRTMDLGSLRRHLKMGPRLTRAWQEMEQI
                     TIGAIEGFCIGGGVALAVALDFRVMGRDAHLRVPEIGLGMNMSWQSIPRMLHLIGPAR
                     TKQAVILANQRISATEAYEWGLVQEVAGPGGAFDAAMALAWKVAEQPPVSVAMTKLTV
                     NRLAYALDDLASHMDVDQFALASLSEDHREGVAAFLERRKPRFKGQ"
     gene            complement(3820..>4384)
                     /locus_tag="VG274_11525"
     CDS             complement(3820..>4384)
                     /locus_tag="VG274_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWE07332.1"
                     /translation="LTFEYCLIAAGASHNRAPSDQRWEKADVLSLDSGGNFHGYIGDL
                     ARMAILGEPDAELQDLLSEIEAIQRAAFEAVRPGRMGGDIYVSAEKLVKQSKQRDNLE
                     FLAHGMGLVSHEAPRLTANGPVAYDDADARRPLEPGMVVSIETTLKHPRRGFIKLEDT
                     VAVTDNGHEIFGEGGRGWNRGGMARAG"
BASE COUNT          832 a         1314 c         1394 g          844 t
ORIGIN      
        1 ctatcgccgc ggcatccgtc acatcgtcgg cggcatgtgc gagaccgatt attccggcta
       61 tccggactgc cgtgatgaaa cgatccgggc gttgcagact gcgctcaacc ttggcatgga
      121 cacgcagttc gagttgcaca ccccgctgat gtggctggac aaggccgcca catggcggct
      181 tgcggatgaa ctcggtggag cggggctggt cggcctgata tgcgaacact cccacacctg
      241 ctatctcggc gaacgcggcg cgcggcatga ttggggtttt ggttgcggcg aatgtccggc
      301 gtgcaaattg cgcgcgaagg gatggtggga atatgcgggg gctaagctcg cgtgagtact
      361 tgtgattggc gcgccttttt ctcgttcgct tcgctctttg cggggcgagg taaggaggcg
      421 cgcgccacta gttcgcaaaa tcctccgccc gcaggtcaat cggcgagccg tgcggggtgc
      481 ggtcggcggc gtgatcccaa gcatcgcgat aaaggtgcaa ggttttggag gaggcgacac
      541 ctttttccgc aaccagtctc tccagtgtcg cgagccaatg acggtaatag gtctcgccgg
      601 tatcgggatc gccgcttgcc tgtgcgcgct tgatctcgtc ggcaagcgca gccgcccatt
      661 cattccagct aaagagcccg cgctcatgca gcgcgagcgc catcgcgaaa gcctgcgctt
      721 cccagggttc gcgaaacacc gggccatcat cgtcgcgggg aataccaggc accgcgctgg
      781 ttgcttcacg cgctgtagcc gcatccatca tgccgtctcc agatacggtt cgaacgcatc
      841 gatggatatc ttcacggtcg gatcagcatc cgggccccat aattcggtcc catcaaacac
      901 gattgtgtaa agccattgcg gattctcgcc ggaaccggcc gcggccgagt ccggaaacac
      961 ctggcagccg tgatcgcgtt cgaccacgcc gacatggcca cgcacgtagc gtggcaggcg
     1021 cgtgtgtgtt cgcggatgga tgttcttggt tcgcacatga tcgccgggtt tgaatttcgc
     1081 cggcgcaggc gccgcgcggg cgaacttgcc gcgcaccatg atgcgctcga tatcgtcgag
     1141 cgtgaatttg ccgcgcttaa gcggtttcgc aggttccgtc ccgtggccgg cggcgatttc
     1201 gccggcagca ataaaaccct tgtcgatcag caggtcttcg agcccgagca gccatttctt
     1261 gtagtatgaa ctggcgagat agacgtcggg cggcaatttc tcccgataga agcgcgaggc
     1321 atcgatgttg aatgcgccgg cggcgcccat cgcacgcacc atcgccagca cgcgcgcctc
     1381 ccattcggcg tgaaacatcg gctcgttcgg ctcgggctcg accttgccga aaccgtccat
     1441 gccgcccatg tcgtgcacgc cattcacgat ggtgcgcccg gcgccttcgg aaatccggta
     1501 ccgatcatcg agtcccgcgt cacgagttcg gcgagctgct gctcgttcca gcccccggtg
     1561 ccctcgggcc gcatcggcag caccaggaaa cgcgtctctg ccgtggaatc ccacacgcgg
     1621 atctcggttt ccttcggcaa gctgacgcca aagtcggcga gcacgccgcg cggatccttt
     1681 actgcgcgcg aacgataggg cgcggccttg taccagaccg gaggaagccc cagcatctcc
     1741 caagggtaac aggagcacag cgtacacacg accatgttgt ggcgctgcca ggtattctcg
     1801 acaaccacga gatgatcgcc gacacggctg acgtgaccga gcgtgctgac ggccctggtg
     1861 ccgtcatcaa gcagtgcctt tttgaactca gggtcggacc aggcttttgc gacgacacgg
     1921 gcgccattat gcgggccgat tttggtttca taggcctgga tgatggcatc aagcgccgcc
     1981 ggttcgacgt agcccttttc ggttaaaacc gtctcgagtg cgcgcacgcg caattcggtt
     2041 tcggacaaat ccgaatggtc gtgatcgtgg tgatggtcgt gatcccggtc gctcatgccg
     2101 cggaggatag gcgaaatact gccgccatgt cgagacgtcg cctgaggcgt cggatagccg
     2161 agatttgatc gctcggcggg atttcgagtt ggccgaggaa cgctcgcact cgtgcgtcat
     2221 tgcccgatcg gcgtgcgtca tttccggaga gatgatccat gcaactcgag aacaagaaga
     2281 tcgccattct cgcaacgcac ggcttcgaac agtcggaact cgaagttccc cgcgacaagc
     2341 tcaaggccgc aggcgccatc gtccatatcg tctcgcttca ggcgggcgag atcagaggat
     2401 gggacatgaa ggactggggc cggccggtta aagtcgacaa gacgttagat caggtttcag
     2461 ccgacgacta cgacgccatc gtgctgccgg gcggccagat caatcccgac ctgctgcgcg
     2521 tggaaccgaa ggcgcttgcc ttcatcaagg acatcttcaa ccagaagaag atcgtcgctg
     2581 cggtctgcca tgcgccctgg ctcctgatcg aaaccggcat cgccaaaggc cgccggatga
     2641 catcgtacaa atcgatcaag actgacgtga agaacgccgg cgcgaaatgg gaggattccg
     2701 agttgtggtc gacaccggcg ttattacgtc gcgcaatccc ggcgacctga atgtctttag
     2761 cgccaagatc atcgaagaag tcggggaagg caagcacgcc aagaggagcg ctgcgtagcc
     2821 tcgggccgcc aaaagttgac atgcgtcacc gcgtgctttt gcgctattgt taaataaaca
     2881 gcgcgatggg aggcggggag tggcggaatt cgtgaccatc gaaaagggcc tcggtcccga
     2941 ggggcgcatc gcggtcgtgc ggttcgatcg cggcgacggc gtcaacgccc tgtcacctga
     3001 ggcgctgcgt caattgaccg gcgcggcgcg cagctttgag gatgatggcg atacctcggt
     3061 tgtggtgctc acgggcggca acaacgcgtt cagcgcgggc ttcgatctca aagaccccga
     3121 aggccgatcg cgccggacca tggatctcgg atcattgcgc cggcacctga aaatgggacc
     3181 gcgactgacg cgcgcctggc aggagatgga gcagatcact atcggcgcca tcgaaggctt
     3241 ttgcatcggc ggcggcgtcg cgctggcggt ggcgctggac tttcgcgtca tgggccgtga
     3301 cgcccatctg cgggtgccgg aaatcgggct tggcatgaac atgagctggc agagtatccc
     3361 gcgcatgctg cacctgatcg ggccggccag gaccaagcag gcggtgatcc tggccaacca
     3421 gcgcatctca gccactgaag cctacgaatg gggtctggtg caggaggtgg ccggtcccgg
     3481 cggagccttc gatgctgcga tggcgctcgc ctggaaagtc gcggaacaac cgccagtgtc
     3541 ggtcgcgatg acgaaattga ccgtcaatcg gctggcgtac gcgctcgacg atctcgccag
     3601 ccacatggac gttgaccagt ttgcactggc gagcttaagc gaggatcaca gggaaggcgt
     3661 ggctgcgttt ctggagcggc gcaagccgag gttcaaagga caatagaagc ggtgaatgcg
     3721 cgccgacggg cctggcgcgg ttactacatc gcggtgatat gctgccaatc caatccagca
     3781 ataggtcggc gatctgagcg ggggcaaacc cggttcttat tatcctgccc gtgccatccc
     3841 gccgcgattc cagccgcggc caccttcgcc gaagatttcg tgcccattgt ccgtcacagc
     3901 caccgtgtct tccagcttga tgaagccgcg gcgcggatgc ttgagggtcg tttcgatcga
     3961 aaccaccatg cctggttcga gcggacgcct cgcgtcggca tcatcatacg cgacaggacc
     4021 gttggccgtg agccgtggag cctcgtggct gacaagaccc atgccgtgcg ccaaaaattc
     4081 caggttgtcg cgttgcttcg attgcttcac gagcttctct gcggaaacgt agatatcgcc
     4141 gcccatcctg cccggtcgaa ccgcttcgaa ggcggctcgt tggatggcct cgatttcgga
     4201 caggaggtct tgaagttcgg cgtccggttc tcccaggatg gccatgcggg cgaggtcgcc
     4261 gatatagccg tggaagtttc ccccggaatc cagcgacagc acgtctgcct tttcccagcg
     4321 ctggtcggag ggagcgcggt tgtgactggc tccggcggca ataaggcaat attcgaatgt
     4381 cagg
//
LOCUS       DASZMC010000568         4874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1872459,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000568 DASZMC010000000
VERSION     DASZMC010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872459"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VG274_11530"
     CDS             <1..301
                     /locus_tag="VG274_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWE07333.1"
                     /translation="REFVNRTKQGFGIPLTKWLSGPLNAQMTHVLSDRRLMEPFDPAE
                     IARVAHEFRTGRTHHTSRLWALFMYGLWRDSEGHAPHFDTAPGRHADVRVESVAP"
     gene            583..933
                     /locus_tag="VG274_11535"
     CDS             583..933
                     /locus_tag="VG274_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07334.1"
                     /translation="MFDTFQLTNQPRPRNVEIMIDQWPDAPEEPDRVRKGGVAIEGFF
                     IDPPRMDEEEPRIARRLKRVYREAGGFRADGPEHVAQRLDHAFLLAFASMKAGEDEKL
                     HYSSVAPAKPAQTS"
     gene            1121..4210
                     /locus_tag="VG274_11540"
     CDS             1121..4210
                     /locus_tag="VG274_11540"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HWE07335.1"
                     /translation="MFPRLCHIGRVTRANTARTALERTAALGFDGVIVEAEGGALCAL
                     GEACHSRGLDLLLDVDFSRWDLRQEFVESHPECFAIRRHSDGEVIDPRSARHDRGHAL
                     LRGGDDLAPVAAWCAERLAEAVAAGAGGFRVLHPGGACAELWSALIGPARARANRSLA
                     LIADTTGLARGELPTLAECGFDFTLSSLPWWDGRANWFIEEHEALSRVAPVMAQIDSP
                     AKLPPASVIARQARLTLAALTGSGIMMPLGFADAPAGHGGGDGVCDRIRAVNAFLGAQ
                     PNSPFLLRKPTGEGAALTVLLRIPGADPTLAGEAFVAFVNPSASISIQPGAEISFALA
                     DFDALRAVLDTGVADSKLPPGAARLFHATRSAAIRQVPTEEASGAANATEAPRIVISD
                     ISPCVAGGRFPARRIVGERIAVEADIFMDGHPVIAAELSWRAEDERGWRRERMIALAN
                     DRWRAEFDLARIGRYRFVISAWLDNYGSFVRDLVRKRDAGQDLALDVQEGLAFLRARG
                     ERASGAALSVLDRIVEVFDELALADRVALLAAPETLEAMARADERRFLVRSPACAIDV
                     ERAAARFASWYELFPRSQTDSARRPGTLCSVIARLPAIAQMGFDVVYLTPINPIGKSH
                     RKGRNNALRAEPGDPGSPYAIGAKEGGHDTVRPELGTIEDFRALVPAAHEHGMEIALD
                     FAIQCSRDHPWLQTHAGWFAWRPDGSIHYAENPPKKYEDIVNIDFYAPAAMPAAWLAL
                     RDIVLFWIDSGVRIFRVDNPHTKPFPFWEWMIADIRARHPNTIFLSEAFTRPKPMYRL
                     AQLGFSQSYTYFIWRNTKRELTDYLTELTTPPVREFFRPHFFVNTPDINPYFLQQSGR
                     SGFLIRAALAATLSGLWGMYSGYELCEAEPLRGREEYQDSEKYEIRPRNWKAPGNIIA
                     EISALNRLRRAEPALQNHLGLTFYNAFNEQVLYYGKSSAGHGDRILVAVCLDPHHTQE
                     ADFEAPLWEWGLADDQALECEDLLSGMHALWQGKMQRLRLTPELPYAIWRVRPARER"
     gene            4213..>4874
                     /locus_tag="VG274_11545"
     CDS             4213..>4874
                     /locus_tag="VG274_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWE07336.1"
                     /translation="MARRRSAALAADPFWYRDAVIYQLHVKAFFDANNDGVGDFPGLI
                     QRLDYLAELGVNAIWLLPFYPSPLRDDGYDISDYRGVHSEYGTIADVRAFIDAAHVRG
                     IRVITELVINHTSDQHPWFRKARRSKPGSAARDFYVWSDTDRAYAGTRIIFLDAEKSN
                     WTWDDEAKAYYWHRFYAHQPDLNFDNPQVLKAVISAMRFWLDMGVDGLRLDAVPYLME
                     REG"
BASE COUNT          839 a         1574 c         1547 g          914 t
ORIGIN      
        1 cagggagttc gtcaacagaa ccaagcaggg cttcggcatc ccgctcacga aatggctgag
       61 cggcccgttg aacgcgcaga tgacgcacgt gttgtccgac cggcgcctga tggagccctt
      121 cgatcccgcc gaaattgcgc gcgtcgccca cgaattccgc accggccgga cgcaccacac
      181 gtcgcgcctg tgggcgcttt tcatgtacgg cctatggcgc gactcggagg gtcatgcgcc
      241 tcatttcgac acggcgcctg gacgacacgc ggacgtgagg gtcgaaagcg tggcgccgtg
      301 aattggagcg ttttctggcc cgcttgcgcg caagtgctgg taggccgaga gacccctcac
      361 ccgcgccgga gcagaaatca ttgcatgtaa ctgcatccct ctcccacgag gggagagggg
      421 aatcggagtc ttgcatcagc agcgggaaat ccgcgcgcgg gcccctctcc cctcggggga
      481 aaggggaaac attcgaggcc tcgctgctgc tccgaaatgg gtgaggggcc actccggctt
      541 tgttatttcg tcgcctgtgt gttgcgtggg tcacgacatt ttttgttcga tacgttccag
      601 ctaaccaacc agcctcgtcc gcgtaacgtc gagatcatga tcgatcagtg gccggacgcg
      661 cccgaggaac ctgaccgtgt gcggaagggt ggcgtggcga tcgaaggctt cttcatcgac
      721 ccaccgcgaa tggatgaaga agagccgcgg atcgcgcgtc gattgaaacg cgtctatcgc
      781 gaggcaggcg gattccgcgc ggacgggccc gagcatgtcg cgcaacgcct cgaccacgcc
      841 tttctcctgg ccttcgcgag catgaaagcg ggcgaagatg aaaagctcca ttattcctcc
      901 gttgcgcctg ccaaacccgc gcagaccagc taattacgca ggcgcccaat ctcaagaatg
      961 acgcccccgc aaaccctgtc cggacccgtt tcgtgctcat cggggaagtt gaccgggacg
     1021 cgaattcaac cggcagcggg aacctggagc gggcctcccg gttagggatt cgggcggctt
     1081 ccgcggcagg ggaggcggtc gcgtgcgcgc cgaggtcgga atgtttccca gactttgtca
     1141 cattggccga gtcacgcggg cgaatacggc gcggacggcg ctggaacgaa cggcggcgct
     1201 tggcttcgat ggcgtgatcg tcgaggcgga gggtggggcg ctttgtgcgc tcggcgaggc
     1261 atgccattcg cgcggcctcg acctgctgct cgacgtggac ttctcgcgct gggacctccg
     1321 tcaggagttc gtcgagtcgc accccgaatg tttcgcgatt cgacggcact ccgatggcga
     1381 ggtgatcgat ccgcgttcgg cacggcatga tcgcggtcat gcattgctgc gcggcggcga
     1441 tgacctcgcc cccgtcgcgg cgtggtgcgc ggagcgcctc gcggaagccg tggcggcggg
     1501 cgccggtgga ttccgcgtcc ttcatcccgg cggcgcctgc gcagaattgt ggtccgcgct
     1561 catcggaccc gcgcgtgccc gcgccaatcg cagtctcgcg ctcatcgccg acacgacggg
     1621 attggcgcgc ggcgagttgc ccacgctggc ggagtgcggt ttcgatttca cgctctcctc
     1681 gctgccctgg tgggacggtc gcgcgaactg gttcatcgag gagcacgaag cgctctcacg
     1741 cgtggcgcca gtgatggcgc agatcgactc gccagcgaaa ttaccgccgg cttcggtgat
     1801 cgcgcgccag gcccggctca cacttgcggc gctgaccgga tcgggcatca tgatgccgct
     1861 cggcttcgcg gatgcgcctg cgggacacgg cggcggcgat ggagtttgcg accgcatccg
     1921 cgccgtcaac gcatttcttg gagcacagcc gaattctccg ttcctgctca gaaagccaac
     1981 cggcgagggg gcagccctca ccgttctcct gcgaatacca ggcgccgacc ccactctggc
     2041 cggagaggct ttcgtggcat tcgtcaatcc gagcgcatcg atttccatcc agccgggagc
     2101 ggagataagt ttcgcgctcg cagatttcga tgcgctgcgg gcggttctcg acaccggcgt
     2161 tgcggattcg aaattgccgc ccggcgcggc gcggcttttc cacgccacgc gctccgccgc
     2221 aatccgtcag gttccaactg aagaagccag cggcgcggcg aacgccacgg aagcgccgcg
     2281 catcgtcatc agcgatatca gcccctgtgt cgccggcgga cgctttcccg cacgccgcat
     2341 cgtcggcgag cgtatcgccg tcgaagccga tattttcatg gatggccatc ccgtcattgc
     2401 tgccgaattg tcctggcgcg ccgaggacga gcgcggctgg cggcgcgagc ggatgattgc
     2461 tctcgccaat gatcgctggc gcgcggaatt cgatctcgct cgcatcggac gctatcgctt
     2521 cgtcatttcg gcatggctcg acaattacgg cagcttcgta cgcgatctgg tgcgaaagcg
     2581 cgacgccggc caggatctcg ccctcgacgt gcaggaaggt cttgcgtttc ttcgcgcgcg
     2641 gggggaacgc gcgagtggcg ccgcactttc cgttctcgat cgcatcgtcg aggttttcga
     2701 cgaactcgcc cttgcggatc gcgtcgctct gcttgcggcg ccggagactc tcgaagcaat
     2761 ggcgcgggcg gacgagcgcc ggtttctcgt gcgaagcccg gcctgcgcga tcgacgtgga
     2821 acgcgcagcc gcgcgcttcg caagctggta tgagctgttc ccgcgctcgc agaccgattc
     2881 ggccaggcgg ccgggcacct tgtgcagcgt catcgcacgc ctgccggcaa tcgcgcagat
     2941 gggcttcgac gttgtctatc tcaccccaat caatccgatc ggcaaaagcc atcgcaaggg
     3001 ccgcaacaat gcgctgcgcg cggagcctgg cgatccgggc agcccctatg ccatcggtgc
     3061 gaaggaaggg ggacacgata ccgtccgtcc cgaactcggc acgatcgagg actttcgggc
     3121 gctggtaccg gccgcgcatg agcacggtat ggagatcgcg ctcgattttg ccattcagtg
     3181 ttcgcgcgat catccctggc tccaaacgca cgccggctgg ttcgcctggc gtcccgacgg
     3241 ttcgattcac tatgcggaga atccgcccaa gaaatacgag gacatcgtca acatcgattt
     3301 ctacgcgccc gccgccatgc cggcggcatg gctggcgctc cgcgacatcg tgcttttctg
     3361 gatcgattcg ggagtgcgga tcttccgcgt cgacaatccc cacaccaagc cattcccgtt
     3421 ctgggaatgg atgatcgccg acatccgcgc gcggcacccc aataccatct ttctctcgga
     3481 ggccttcacg cggccgaaac cgatgtatcg cctggcgcag ctcggcttct cgcagtccta
     3541 cacctacttc atctggcgca acacgaagcg cgagctgacg gattatctga ccgaactgac
     3601 gacgccgccc gtcagagaat tcttccggcc gcatttcttc gtcaacacgc ccgacatcaa
     3661 tccgtatttc ctgcagcaat ccggccgcag cggatttctg atccgcgccg cgctggcggc
     3721 gacgctgtcg ggtctttggg gcatgtactc gggatacgag ctttgcgaag ccgagccttt
     3781 gcggggccgg gaggaatacc aggattccga aaaatacgag atcaggccgc gcaactggaa
     3841 ggcgccggga aacatcatcg cggagatttc cgcgctcaac cggttgcgca gggcggagcc
     3901 ggcgctgcaa aatcacctgg gacttacttt ctacaacgcg ttcaatgagc aggtacttta
     3961 ttacggaaag tcttccgccg ggcatggcga ccgcattctc gtcgcggtct gcctcgatcc
     4021 gcatcatacc caagaggccg atttcgaggc gccgctttgg gaatggggtc tcgcggacga
     4081 tcaggcgctc gaatgcgaag acctgctcag cggcatgcat gcgctctggc agggtaagat
     4141 gcagcgcctt cgcctcacgc cggaattgcc ctatgcgatc tggcgcgtga ggccggcgcg
     4201 ggagcggtga gcatggcgcg ccggcgatcg gccgcacttg ccgccgaccc tttctggtat
     4261 cgcgatgcgg tcatctatca gctgcacgtc aaggccttct tcgacgcgaa caatgacggc
     4321 gtcggcgatt tccccggcct gatccagcgc ctcgactatc tggcggagct gggggtcaat
     4381 gcaatctggc tcctgccttt ctatccttcg ccgcttcgcg acgacggcta cgacatctcg
     4441 gattatcgcg gcgtgcattc cgagtacgga acgatcgccg acgtgcgcgc gttcatcgac
     4501 gcggcgcatg tgcgcggcat tcgcgtcatc accgaactgg tgatcaatca cacctccgac
     4561 cagcatccct ggtttcggaa ggcgcggcgc tcgaagcccg gctcggcggc gcgcgatttc
     4621 tacgtgtggt cggacaccga cagggcctat gccggaacgc ggatcatttt cctcgacgcg
     4681 gaaaaatcga actggacgtg ggacgacgaa gcgaaggcct attactggca ccgattctat
     4741 gcccaccagc ccgatctcaa tttcgacaac ccccaggttc tcaaggccgt catttcggcc
     4801 atgcgcttct ggctcgacat gggagtcgac ggcctgcggc tcgacgcggt gccctacctc
     4861 atggagcggg aagg
//
LOCUS       DASZMC010000569         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4374399,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000569 DASZMC010000000
VERSION     DASZMC010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4374399"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VG274_11550"
     CDS             complement(<1..1220)
                     /locus_tag="VG274_11550"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HWE07337.1"
                     /translation="MSLEGAIAANRFGLGARPGEIAHASENPKAWLMSQMNGPAPQLV
                     PLDGQPFPNAGKLVSEEDDYKVAKFMDKGNKLARKKQQEKDIAKVAANTKPSDAMTSD
                     SMRAADDTVDTKKHGKGGGRDIEPVRKIEYTNEMAARFALGFTTDRPFAERLVRFWSN
                     HFTISDKKKKVLLFAGDFEREAIRPNINGTFEDMLFAVVLHPAMQIYLDNWRSIGPDS
                     SAGKDRGKGLNENLGRELMELYTLGVDGGYTQADVIAMAKLLTGFGVYAGHANGFGFF
                     PERHEPGPITLRGKTYPAGWDGSVAAIKDLAHDPATARNIARKLAAHFISDNPPKESV
                     ARLEKAFNDSHGNLHAIAQAIVDDPAAWNAEQKKMRTPVEYVTAAMRLCGWSQARDMD
                     HERRKLRGLLSSVRV"
     gene            1295..>1841
                     /locus_tag="VG274_11555"
     CDS             1295..>1841
                     /locus_tag="VG274_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWE07338.1"
                     /translation="MTTPDNTDKSSLTYKDAGVDIDAGEELVSRIGPAAKATRRPGAD
                     ADLGGFGGLFDLKAAGFKDPIIVASTDGVGTKLKIAIETRLFDGIGQDLVAMCVNDLV
                     VQGAEPLFFLDYYATGMLDVDAAARVVESIARACKESGCALIGGETAEMPGFYPEGEY
                     DLAGFIVGVVERERIVTGKTVE"
BASE COUNT          365 a          553 c          539 g          384 t
ORIGIN      
        1 acacgtacgg acgagagaag accgcgcaac ttgcgacgct catgatccat atcgcgcgct
       61 tgcgaccagc cgcaaagacg catcgccgcg gtcacatatt caaccggtgt acgcatcttc
      121 ttctgctccg cgttccaagc agccggatcg tccacgatcg cctgcgcgat ggcgtgcaaa
      181 ttcccatgtg aatcgttgaa tgccttctcc agacgcgcga ccgactcctt tggaggattg
      241 tcggagataa aatgcgccgc aagcttgcgt gcgatgttgc gtgctgtcgc gggatcgtgc
      301 gcaagatcct tgatcgccgc cacgctgcca tcccaaccgg cgggataagt cttgccgcgc
      361 aacgtgatcg gaccgggttc gtggcgttcc ggaaagaagc cgaaaccgtt ggcatgaccc
      421 gcgtagacac caaagccggt taggagcttt gccatcgcaa tgacgtcggc ctgcgtgtag
      481 ccgccatcga cgccaagcgt atagagttcc atcaattcgc ggccaagatt ctcattcagc
      541 cccttgccgc gatcttttcc ggcactcgaa tccggcccga tcgaacgcca gttgtcgagg
      601 tagatctgca tcgcgggatg cagcacgacc gcgaacaaca tatcctcgaa ggtgccgttg
      661 atgttgggac ggatcgcctc gcgttcgaaa tcgccggcga acagaagaac ttttttcttc
      721 ttgtcggaaa tcgtgaagtg gttgctccaa aagcgcacaa ggcgctccgc aaaaggccgg
      781 tcggtcgtga agccgagcgc gaaccgcgcg gccatctcat tggtatattc gatcttgcga
      841 acgggttcga tatcgcgacc gccaccttta ccatgctttt tcgtgtccac ggtatcgtcc
      901 gccgccctca tagaatcgga tgtcatggca tcagagggct ttgtgtttgc agcaactttc
      961 gcaatgtcct tttcctgctg cttcttgcgg gcaagcttgt tgcccttgtc catgaatttg
     1021 gcgaccttgt agtcgtcctc ctcgctcacc agctttccgg cgttcggaaa cggctgaccg
     1081 tccagcggca caagctgcgg cgcgggaccg ttcatctggc tcatcagcca ggccttggga
     1141 ttttcgctcg cgtgcgcgat ttcacccggc cgggcgccga gtccgaatcg attggcggcg
     1201 attgcgcctt caagcgacat tgttgctccg attcaatggt tgcgcggttc catatgcgtg
     1261 attaaacgga tggcgaaccc tgttccgtcg cgccatgaca acgccagaca ataccgacaa
     1321 aagcagcctc acttacaaag acgccggcgt cgatatcgat gcgggcgaag aactcgtgtc
     1381 ccggatcggg ccagccgcga aagcgacgcg gcgtccaggc gcagacgcag atctcggtgg
     1441 cttcggcggt ttgttcgacc tgaaggccgc tggcttcaag gatcccatca tcgtcgcctc
     1501 gacagacggc gtcggcacca agctcaagat cgccatcgaa acgcggctgt ttgatggcat
     1561 aggccaggac ctcgtcgcga tgtgcgtgaa cgaccttgtc gtgcagggcg cggaacctct
     1621 gttcttcctc gactactacg cgaccggaat gctcgacgtc gatgcggcgg cgcgcgtggt
     1681 cgaaagcatt gcgcgcgcat gcaaggaaag cggatgcgcg ctgatcggcg gcgagacggc
     1741 ggagatgccc ggcttttatc ccgaaggcga atacgatcta gcaggattca tcgtgggcgt
     1801 ggtggagcgc gaacgcatcg tcaccggaaa gaccgtcgaa c
//
LOCUS       DASZMC010000570        20884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2155305,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000570 DASZMC010000000
VERSION     DASZMC010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 20884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20884
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155305"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1133)
                     /locus_tag="VG274_11560"
     CDS             complement(111..1133)
                     /locus_tag="VG274_11560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWE07339.1"
                     /translation="MSTTIATSNGSLREKTVGFMLAHEQFPVPDLVELGRRAASVGFG
                     LLATSDHFQPWQANEAHSGLAWVTMGALGERANSAWIGTTVTCPTLRYHPAVVAEAFA
                     SLSLLYPGRVFLGVGSGEALNEQAATGEWPHWKERWERLIEAIAIIRALWSGERVNHK
                     GRYYTVDGRLYDVPPEPIPLLTAANGPRAMRLAGRHGDGLITDPLTWKQHKSEWEQGV
                     RESGRTPAEMPVLVEQFVVVGDRAQAEESAKLWRFIPRAFKKYVGVPDPVEIQREAEA
                     DLQLEQVYKDWPCGTDPDVHASAIEALFASGVTIVNIHSGQKDQENVVDFYGNDVLPR
                     LGGYMR"
     gene            1275..3437
                     /locus_tag="VG274_11565"
     CDS             1275..3437
                     /locus_tag="VG274_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWE07340.1"
                     /translation="MPISRAFMCMLLGSLAVAAPAASKPRAADFTMAQVLHYPFADQL
                     AAAERADRIAWVRNVGGVRNVWFADGPVFRPHQVTQYSADDGQEITQLAFSPDGKHLV
                     YVRGGDHDANWPAEGNLAPDPDASPQQQMVTIWAVSLAGAAPVKIAAGDTPAISARGT
                     LAYTINDQVWTAPLDGKGKPARLFFDRGKDSDLHWSPDGSRLAFVSNRGDHAFIGEFV
                     SKDKPLIYLAPSTSSDLSPRWSPNGTKIAFARRQGDGGPPLPILKQTPQPWSIWVTDA
                     ADGTGHAVWSSPDTLAGSWPDVEGEANLHWAAGDRVVFLAYLDDWPHLYSVAATGGAP
                     LLLTPGAFMVEHVALSRDRKFIIYDANTGKTADDDDRRHLFRVLVDRAAPVALTSGET
                     IEWRPVAASRDRVAFVSTGAQHPPEIGMVGIDGAHRTTLDAGALPADFPVAELIVPKS
                     VAFAAADDTRVHGQLFQRRDGGGAKPGIIFVHGGPPRQMLLGWHYMDYYSNAYAVNQY
                     LATHGFIVLSVNYRLGIGYGRKFHQPDHAGPTGAAEYQDVVAGAHFLQHTAGVDAQRI
                     GMWGGSYGGYLTALALARNSDIFKAGVDLHGVHDWSRLLDELGGKPVTRFEKGDRDAA
                     LRVAWTSSPDADIDRWKSPVLLIQGDDDRNVPFQQTIDLARRLQARHLPFEELVIPDE
                     IHGFLRHESWLSADTATAAFLARKLATGTYTRSGALER"
     gene            3793..5064
                     /locus_tag="VG274_11570"
     CDS             3793..5064
                     /locus_tag="VG274_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWE07341.1"
                     /translation="MNVEALKKDAAAAVDSMRAELLELSHAIHQEPELALEEFKAAVR
                     LSDAVAAHALPVQREAFGVKTAYVADFGTDSGPAIAVLSEYDALPGVGHACGHNIIAT
                     AGLGAALALAKLGANLPGRVRYLGTPAEERFGGKEILARAGAFEGVDAAMMIHPANVD
                     LVTMPCIAISEVEVTYFGRAAHASAMPYRGLNALDAVVTAYQSIAQLRQHIRQTERIH
                     GIITDGGLAANIVPERAACRFYVRAADVHELAALKKRVQACFEAAALATGCTVKTGWG
                     AVDYLDLKTNWPMAEAMAKNIESLGRELVPLGSIPPGFAGSTDMGNVSHRVPSIHPML
                     AVAPANVIIHNAEFARWAASERGDAAVLDGAKSLAWTALELKGDPALLERTRADFAAT
                     AEISRCALARLEEKVADAHPAVHAHPGCGCA"
     gene            5068..5433
                     /locus_tag="VG274_11575"
     CDS             5068..5433
                     /locus_tag="VG274_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HWE07342.1"
                     /translation="MKPWLAVAAINGFLAVAAGAFGAHALQGRIDAHALAVFETGARY
                     QMYHALAIGLAVLSLRGTPGAELACALFLLGIVLFSGSLYALALSGMRAFGLVTPLGG
                     LAFLAGWGTLAYSALKTVS"
     gene            5445..6413
                     /locus_tag="VG274_11580"
     CDS             5445..6413
                     /locus_tag="VG274_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE07343.1"
                     /translation="MIDFPAPTMIRTNGIDMAVYEAGPRDGFPVVLCHGFPELAFSWR
                     HQLPALAEAGYRAIAPDQRGYGKTSRPVEMCDYDMAHLTGDLVGLLDAFGIEKAVFCG
                     HDWGGSVVWAMPLYHPDRVAGVIGVNTPFFPRPPVDPIALMRARFGDDMYIVFFQKPG
                     EADAILAADVGKTFRFFMRRNVATADEYAKRPQAERNLKLVRALQSDESRWRGEALLD
                     AQEMKVFVDIFTRTGFTGGINWYRNISRNWELSEGKEPIVGAPALMIMAEDDVILAPS
                     MTEGMERFVPDLEKILLRRCGHWTQQEKPDETNDAMIGWLKRRFPC"
     gene            6448..7344
                     /locus_tag="VG274_11585"
     CDS             6448..7344
                     /locus_tag="VG274_11585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWE07344.1"
                     /translation="MGDTASDNFSYAEPDDPRLKRFVIRLIERMSGQPYLKWLYDDHL
                     TNPLPGESFWDAAVRRLELKVVCNEEVLQTWPKEGPLIVVSNHPFGVLDGVVICHLVS
                     RVRGDFRVLTNSVLARAEQVRKFLLPIDFTESPEAVRTNIESRAAAKKHLLEGGCLIV
                     FPAGGVSTTPTVWHRRAVDTEWKTFTARLIRQAKAPVASVFFAGQNSRMFQLASHIST
                     TLRLSLLFKEVHDKIGSELHIRIGVPVPFEKLPATADRQEFMRQLRETTYRLGEGVHP
                     PPRPRVKRPRRAPAHSRKPHAK"
     gene            complement(7549..8112)
                     /locus_tag="VG274_11590"
     CDS             complement(7549..8112)
                     /locus_tag="VG274_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWE07345.1"
                     /translation="MRNESRSAWKGARSTLDLDRFLPYRLSVLTNRVSNAIARHYSER
                     FDLSPPEWRVMAALGQASGLSAREVAVRTEMDKVQVSRAVARLMRGKRMQKQTDMSDG
                     RVTRLSLSARGRAIYSEIVPLALALEEQFLAALGPRERAALDVLMSKLAQQVLSLGDF
                     PPRLRERSAPPGPAEGRPEDKLRPGRE"
     gene            complement(8102..8761)
                     /gene="maiA"
                     /locus_tag="VG274_11595"
     CDS             complement(8102..8761)
                     /gene="maiA"
                     /locus_tag="VG274_11595"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HWE07346.1"
                     /translation="MPGPEFVLYGYFRSSAAFRVRIALNLKGIKPELRFVHLLKGGGQ
                     QHGADYSAVNPQQLVPTLVHDGHAIGQSLAIVEYLDELRPDPPLLPRDAAGRARVRQL
                     ACMVGCDIHPLNNLRVNLYLRDRLGANEEARLRWQRHWMELGFAALETMMAQSPQTGT
                     FCHGDAPTLADICLIPQLANARRVHLDLGAYPTLLRIEEQALALPAFEAALPKNQPDA
                     E"
     gene            complement(8748..9731)
                     /locus_tag="VG274_11600"
     CDS             complement(8748..9731)
                     /locus_tag="VG274_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWE07347.1"
                     /translation="MKLASLRHGRDGLLVVADRDLARCVAVPHIASTLQEAMDDWHHA
                     APRLMRVYDLLNEGAADGARPFASEDCAAPLPRAYQWADGSAYVTHVELVRKARGAEM
                     PASFWTDPLMYQGGSDGFIGAREPILAADESFGIDFEAEVAAITDDVPMGTAPDAALP
                     HIKLLMLVNDVSLRNLIPAELAKGFGFFQSKPASAFSPVAVTPDELGEAWSEGKVHLP
                     LSVHLNEAQFGAPNAGDDMTFSFAQLIAHAAKTRGLGAGSIIGSGTVSNRDASRGSCC
                     IAEKRTREQLETGRPATPFLHFGDRVRIEMLDPRGHSIFGAIDHEVVRARP"
     gene            complement(9728..10876)
                     /locus_tag="VG274_11605"
     CDS             complement(9728..10876)
                     /locus_tag="VG274_11605"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HWE07348.1"
                     /translation="MSAKNWIEFPRVEGVTSRQAHADLPDGTFEREMGREGFFGPATH
                     FYHRHAPTGWTSIEGALRPRAFDAAKAAEVSSTPFGAAPILASQQCAVAMWTCAAPMR
                     RLARNADGDTLLFIHEGAGDLFCDFGHLGWHDGDYILLPRGTMWRIEPKPRAVILTIE
                     AVDGAYKLSERGLLGPHAIFDPAALETPRLDEAFAAQADGEWRVDVKRRKQVSTIVYP
                     FNPLDAAGWKGNLAPVRLNWRDIRPVMSHRYHVPPSVHSTFVADRFVVCTFVPRPIET
                     DPGALKVPFFHSNDDYDEAIFYHRGEFFSRDNIHPGMMTVHPGGITHGPHPRAFAAGA
                     RAARKETDEVAVMIDSRDPFELAPQAEAHEDRAYADSWGEKPESSRRT"
     gene            complement(10873..11937)
                     /gene="hppD"
                     /locus_tag="VG274_11610"
     CDS             complement(10873..11937)
                     /gene="hppD"
                     /locus_tag="VG274_11610"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HWE07349.1"
                     /translation="MAALWDNPMGTDGFEFVEYTAPDTGALHALFERMEFRAVARHRS
                     KAVTLYRQGGINFVVNAEPDSHGSRFARAHGPSACAMAFRVHDAGKAYARALELGAVP
                     FHNQVGPMELNIPAIEGIGGSVIYLVDRYGDRSIYDVDFVATDAAKGFAHDGAGLAAI
                     DHVTHNVFRGNLTRWAGFYEKLFNFREVRYFDIEGKLTGLKSRAMTSPCGTIRIPINE
                     SSDDKSQIEEYLHLYKGEGIQHIALATEDIYGSVAKLRGRGVAFQDTPDTYYERVDNR
                     VKGHGEDVERLKRERILLDGAPTEDQGLLLQIFTQNAIGPIFFEIIQRKGNEGFGEGN
                     FQALFESIEADQIRRGVFEA"
     gene            12342..13268
                     /locus_tag="VG274_11615"
     CDS             12342..13268
                     /locus_tag="VG274_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07350.1"
                     /translation="MTNRIPVGQTISGAYNFAFTRFLSILAIAWLPYAVFAVIAVGLA
                     LLAVPDLPRMLTTHDIDIEAMMGLSRVVLLIAVIGFIMSSMITVGLQRQALGKQTAPV
                     WYYFSLGAPVWRMAGAFFLAGLVIVFIAIVISAACAAIWFAAGTIPGAVWPIRVVAIA
                     AGTCFLIYIATRLLFFLPAVVTAEESIGIERAWILGRGNFWRIFLVWLAVIVPVAIAF
                     WIVSRAVLGPFVPWPHAHMGTREMIRMFVTQSSAFAPLVLVVEFVERVVILGVANGAV
                     ANAYLAVAGAPPVAPAAARQGSEPGFGSGTGA"
     gene            13315..14175
                     /locus_tag="VG274_11620"
     CDS             13315..14175
                     /locus_tag="VG274_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07351.1"
                     /translation="MIRIPVAETIRFAYAFAFGQIGAIIGLVWLPLLVAALLQFLPYA
                     IGTAYPPDASDAAGATGAALLNLAFVAATLVLYAMNCVSVTRQALGLRQGAATVHFSL
                     ARPEWRMFAAILVCGLITVSVLMFCLLVATMPGRLAGAPGLLEVVAALAGLAAAVWVT
                     LRLIFFVPPVVVAEERIDLPRAWMLSRQNFWRILAIVLAATVPFLVIQGVVFLILLGP
                     GFVAPLPTNTAAAAVALTRRLALFDRHMPTVIGFALVLAPFNLGLTLGASAFAYRALV
                     PARPSVRSAG"
     gene            complement(14341..14937)
                     /locus_tag="VG274_11625"
     CDS             complement(14341..14937)
                     /locus_tag="VG274_11625"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWE07352.1"
                     /translation="MAQPKKNARGRPRDPGTRRRILAAAAKLLDEGGLGAVTMEAIAA
                     LAGVGRPTIYRGWPNAHAVAMSAFLERDRTAPPSHKSRSALAALRSRLRELAETLATR
                     AGRSTAMMIAAAQNDSELAKVFRTHFVMKSREEGRALLARAAAEGGIRRKIDIEAALD
                     LIYAPFYFRLLIGHAPLTSRDTDAILDLALAGLAARRR"
     gene            15021..15494
                     /locus_tag="VG274_11630"
     CDS             15021..15494
                     /locus_tag="VG274_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWE07353.1"
                     /translation="MSATSPTRGSPVRGSITPYIIVKGADAAIAFYTKVFGATEESRL
                     SDPDGRIGHAELAIGDGHLMLADETAAFGALSPPAIGGTPVKLHLPVDDVDAVMERAR
                     QAGATILRPAQDQFYGERSGMLADPFGHQWFLGTRKEAISPEEMQKRYTDSFKVA"
     gene            15518..16762
                     /locus_tag="VG274_11635"
     CDS             15518..16762
                     /locus_tag="VG274_11635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HWE07354.1"
                     /translation="MRSRKAGIALVLAVVYVDMLGIGLAFPILPRLVQQFEHGDVSRA
                     AWIYGLLAAAYALMQFMLAPMLGALSDRFGRRPVLLLSLLGMGLNYAVLAAAPSLAWL
                     AAGRIVAGAMGASYSTASAYLADITPPEKRAQSFGLIGAAFGFGFITGPALGGLLGDI
                     DLRLPFVVASVLSFANLALGYFILPESLPPEHRKDFSLARANPVGALGTIGRYPAIGG
                     LLLVFVLATFANKVAETTWVLFATYKFHWNAASVGLSLAMVGVIFVIGQGGLVRVVVP
                     RLGERRSILLGLFVNAVVFLLYGGVPEGWMVYPAMVLGLFGWTIAQPASMGVMSRALP
                     ANEQGLLQGGIASVNSLAAVIGPPVWTSIFAYFVGPATSVVIPGAAFDGAALVFFIAL
                     VLAIRTLPAESMADVALANTGT"
     gene            17131..17598
                     /locus_tag="VG274_11640"
     CDS             17131..17598
                     /locus_tag="VG274_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07355.1"
                     /translation="MGVAAHPPFISMPVGALDRKGRVCIPASYRQILTAQSTNGVYVC
                     PSFYEPALECFGENVLQEFHQQQAGDDPFFAETHDVKAFSVLAMTQLLALDETGRVRL
                     PDALIAHAGLVENVTFVGMGRKFEIWDTQRFAPVLNERLASARALRKSQPVAS"
     gene            17595..18533
                     /gene="rsmH"
                     /locus_tag="VG274_11645"
     CDS             17595..18533
                     /gene="rsmH"
                     /locus_tag="VG274_11645"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWE07356.1"
                     /translation="MNQGHAPVLLAEVLDALAPRDGAHYVDGTFGGGGYAQAILDRAK
                     CKVLGIDRDPDAIIRGAALKERYAGRLALAQGRFSAMEQLLAEQHETGSDGVVLDLGV
                     SSFQLDEPGRGFSFRGDGPLDMRMERQGSSAGDFVNTAGEAELADVIARLGEERQARR
                     IARAIVAARPLSRTGELAEIVVEALGPGAARHKIHPATRTFQALRLHVNNELGELASG
                     LAAAERVLRPSGRLAVVSFHSLEDRIVKQFLVERSGRAPVGSRHAPASPAARAPTFRL
                     LPPQPRMSSAAEIARNPRSRSARLRAAERTSVPLAA"
     gene            18543..18923
                     /locus_tag="VG274_11650"
     CDS             18543..18923
                     /locus_tag="VG274_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07357.1"
                     /translation="MIRILNFFCFAAAALCCLALYHISEQTRVSRIHLLAVERQIADE
                     HGAMKVLQADWERVSEPSHIQALAQTKLGLSDVPAVALASLELLPRRGETPLAGSPVQ
                     TASITVGTISSDSHLRLAAAHAGN"
     gene            18910..20568
                     /locus_tag="VG274_11655"
     CDS             18910..20568
                     /locus_tag="VG274_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWE07358.1"
                     /translation="MRGTDGQIYAPVERRQDGREAVSVIQRRIVVAAGICVMAFALIG
                     VRLVDVGIMKGSVTGASAPIGEQPQPARADILDRNGVVLARDLPVGDLYARPHVFADR
                     REAARQLAALAGVSSERLARAFDSRHNYVLVARQLVPDVQDKIRRLGLPGLEFEPAAK
                     RYYPEGLAAVQVLGTTDPDGNGVDGLELGLDSTLGQAEPGAGIAVSLDMRVQYALAHE
                     VEQARETFGARAAGGIVLNVNTGEILAMASLPDAPGETGAAANADPRRNRMAQDVYEL
                     GSVFKIFSFALAMEDHTIRLDEMFPIAAGFHIGRYTIHDAEHMPATLAARDILAQSSN
                     VGTAQIALRSGPERQRAFLAHLGLLAPVRTELPERAAPLVPRHWSEVETATIGFGHGI
                     SVSPLSFVTAAAALVNGGRRIVPTFLKQASDARGEQLIKPETSATMRELLRYVVTNGT
                     GKKADVPGYDIGGKTGSAEKVSGRGYVAHRLLTSFCAVFPIENPRYLVFVMLDEPHGT
                     KATFGLALAGFTAAPLAGRVVAHIAPMLGMPTDASAVVQAKDNS"
     gene            20568..>20884
                     /locus_tag="VG274_11660"
     CDS             20568..>20884
                     /locus_tag="VG274_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HWE07359.1"
                     /translation="METQSGAIVAAGMASIAPMQEFSEFAGLASDSRSVGPGYLFAAL
                     PGTQADGALFIADAVRRGAIAVLGRPSVRPEAEKHGVPLIADDNPRLKLAKIASEHFG
                     AQPR"
BASE COUNT         3408 a         6823 c         6721 g         3932 t
ORIGIN      
        1 agcgcgccca taccgccaaa gagggacagg gaagtgaacg aggcttgcag cgatcggccg
       61 ttgccctgca cagatccatt cgaacttcaa gcaccacagc ccgtcccgtt ctagcgcata
      121 taacctccaa gcctgggcag cacgtcgttg ccgtagaaat cgacgacgtt ctcctggtcc
      181 ttctgtcccg aatggatgtt cacgatcgtg acgccgctgg caaagagggc ttcgatcgcc
      241 gatgcgtgaa catcgggatc cgtgccgcag ggccaatcct tgtaaacctg ttcgagctgc
      301 agatccgctt cggcttcgcg ctggatttcc acgggatcgg gaaccccgac atatttcttg
      361 aacgccctgg gaatgaatct ccacaatttg gccgactctt cggcttgggc gcgatcgccc
      421 acgacaacga actgctcgac gaggactggc atttcagccg gagtccggcc cgattcgcgg
      481 acgccctgtt cccattcgga tttgtgctgc ttccaggtca gcggatcggt gatcagcccg
      541 tcgccgtggc gcccggcgag ccgcatcgcc ctggggccgt ttgcggcagt gaggagcgga
      601 atgggctccg gcggtacgtc gtaaagcctg ccatcgaccg tgtagtacct gcccttatga
      661 ttgacgcgtt cgccgctcca cagcgcgcga atgattgcga tggcttcgat gagccgctcc
      721 cagcgctctt tccaatgcgg ccactcgccc gtggcggcct gttcgttgag cgcttctccc
      781 gagccgacgc cgagaaagac gcggcccgga tagagcaggc tcaacgaggc gaaggcttcg
      841 gcgacgacgg cgggatggta gcgaagcgtc gggcaggtca ccgtcgtgcc gatccaggcg
      901 gagttcgcac gctcgccaag cgcacccatc gtcacccacg ccaggcccga atgcgcttcg
      961 ttggcctgcc agggctgaaa gtggtcgctc gtcgccagca atccgaagcc tacgctggcg
     1021 gcgcgccggc caagctcgac aagatcgggc acgggaaact gctcatgcgc cagcatgaaa
     1081 cccaccgtct tttcacgcag cgaaccgttc gatgtcgcaa tggtcgtgct cattgtttgc
     1141 ttcatccttt cgtgcggctg cgcttcgcga acgcgttatc gcggatcggg ttccgggcgc
     1201 cgcttgcggg cgcaggtccg gcaggcgaaa ctgtggttcc cgctttgcat gattctcctt
     1261 tcaggactcg atcgatgcca atttcgcgcg cgttcatgtg catgcttctc ggctctctgg
     1321 ctgtggcggc gcccgccgcg tcgaagccgc gcgctgcgga tttcaccatg gcgcaggtgc
     1381 tccactatcc tttcgccgac cagctcgcag ccgcggaacg cgccgaccgg atcgcatggg
     1441 tgcgcaatgt cggtggcgtg cgcaatgtat ggtttgccga cggcccggtg ttccggccgc
     1501 atcaggtgac gcagtacagc gcagacgacg ggcaggagat cacccagctt gctttctcgc
     1561 ccgacggcaa acatctcgtc tatgtccgcg gtggcgacca cgacgcgaac tggccggcgg
     1621 aaggcaatct cgcgcccgat ccggacgcct cgccgcaaca gcaaatggtg acgatctggg
     1681 ctgtatcgct cgcgggggct gctccggtga agatcgcagc cggcgacacg cccgccattt
     1741 ccgcgcgcgg cacgcttgcc tacacgatca acgaccaggt gtggaccgcg ccgctcgacg
     1801 gaaagggcaa gcccgcgcgc cttttcttcg accgcgggaa agacagcgac ctgcactggt
     1861 cgcccgatgg aagccggctc gccttcgttt ccaatcgcgg cgatcacgcg ttcatcggcg
     1921 agtttgtgtc gaaggacaag ccgctgatct atctggcccc gtccacgagc agcgacctct
     1981 cgccgcgatg gtcgcccaac ggcacgaaga tcgcgtttgc gcgacggcag ggcgatggcg
     2041 gcccgccgct gccaatcctg aaacaaacgc cgcagccctg gtcgatctgg gttaccgatg
     2101 ccgcggacgg cacgggccac gcggtatggt cgagcccgga caccctcgcc ggctcatggc
     2161 ccgatgtcga aggcgaggcg aatctccatt gggcggcagg agatcgtgtg gtgtttctcg
     2221 cctatctcga cgattggccg catctctatt ccgttgcggc cacgggagga gcgccgctgc
     2281 tgctcacgcc gggagcgttc atggtcgaac acgtcgccct gagccgtgac cgcaagttca
     2341 tcatctacga tgcgaacacc ggcaagacgg cggacgacga cgaccgccgc catctcttcc
     2401 gagtgctggt cgatcgcgcg gcgccggtgg ctctgacttc gggagaaaca atcgaatggc
     2461 ggccggtcgc ggcatcccgg gatcgcgtgg cgttcgtgtc gacgggcgcg cagcacccgc
     2521 ccgagatcgg catggtcggc atcgacggcg cgcatcgcac gacgctggac gccggtgccc
     2581 tgcccgcaga ctttcctgtt gctgaactca tcgttcccaa atccgtggcg ttcgctgcgg
     2641 ccgacgacac gcgcgtgcac ggtcagttgt ttcaacgccg ggacgggggc ggcgccaagc
     2701 ccggcatcat tttcgtgcat ggcggcccgc cgcggcagat gctgctcggc tggcattaca
     2761 tggattatta cagcaacgcc tatgcagtga atcagtatct cgcgacgcac ggctttatcg
     2821 tgctgtcggt caattacagg ctgggcatcg gttatggccg gaagttccat cagcccgatc
     2881 acgcaggacc gacaggcgcc gccgagtatc aggacgtcgt cgcaggcgcc catttcctgc
     2941 aacacacggc cggcgtggac gcgcagcgca tcggcatgtg gggcggttcc tacggcggct
     3001 atctcaccgc gctcgcgctg gcgcgcaact ccgacatctt caaggccggt gtcgatcttc
     3061 acggcgtgca tgactggtcg cggctgctcg acgaactggg tggcaaaccg gtgacgcgct
     3121 tcgagaaagg cgatcgcgac gcggcattga gggtcgcatg gacctcttcg ccggatgccg
     3181 acatcgaccg ctggaaatcg ccggtgctgc tcatccaggg cgacgacgac cgcaatgtcc
     3241 cgtttcagca gacgatcgat ctcgcgaggc gcctgcaggc gcgtcacctg cccttcgagg
     3301 aacttgtgat ccccgatgag atccacggct tcctgcgtca cgagtcctgg ctgagcgccg
     3361 acaccgcgac ggcggcgttc ctcgcacgaa aactcgcaac cggcacttac acaagaagcg
     3421 gtgcgttgga gcggtagtgt tcggtttcca acgcttcgta tccaaaaaaa gattgtcatg
     3481 gccccgctgg ttcagggaat caatgacgag tgacacaaag acagatttaa cgcagagtac
     3541 gcggaggcag cgcagtggac gcagagttgg tgcgccgcag gcgtgcgatt cttaccctgc
     3601 gccctccgcg catcctccgc gccctctgcg ttaaaaatct gccgacacac gattcaccaa
     3661 agaccaaaag ctggtaccag gcgatgattt tgggcgcggt ttatcgcgtc gccgaacgga
     3721 gccgatcgca ggttttgggc tttcgcgggg gttccgctat tgtgtgcggc atccgtccgg
     3781 agaagccgtt tcatgaatgt cgaagcgttg aagaaggacg cggccgctgc ggtcgattcg
     3841 atgcgcgccg aacttctgga actcagtcac gcgatccacc aggagccgga gctcgcgctc
     3901 gaggaattca aggctgcggt tcggctttcc gatgcggttg cggcgcacgc tctgccggtg
     3961 cagcgcgaag cgttcggcgt gaagaccgcc tatgtcgcgg atttcggcac ggacagcggt
     4021 ccggcgatcg cggttctctc cgaatatgac gcgctgccgg gcgtcggcca tgcctgcgga
     4081 cacaacatca tcgccaccgc ggggctggga gcggcgctgg cgctggcgaa actcggcgcc
     4141 aaccttccgg gccgcgtgcg ctatctcggc acgcccgccg aagagcgttt tggcggcaag
     4201 gaaattctgg cgcgcgccgg cgccttcgaa ggcgtggacg cggcaatgat gatccatccc
     4261 gccaatgtcg atctcgtcac catgccgtgc atcgccatct cggaggtgga agtcacctat
     4321 ttcggccgcg ccgcgcacgc ctctgccatg ccctatcgcg gcctgaatgc gctcgacgcg
     4381 gtggtcaccg cctatcagtc gatcgcccaa ctacgccagc atatccgcca gaccgagcgc
     4441 attcacggca tcatcaccga tggggggctt gccgcgaaca tcgtgcccga acgcgccgcc
     4501 tgccgcttct atgtgcgcgc cgcagacgtg cacgagctgg cggcgctgaa gaaacgcgtg
     4561 caggcctgtt tcgaggcggc agcgcttgcg acgggctgta cggtcaagac aggctggggc
     4621 gctgtggact atctagacct gaagaccaac tggccgatgg ccgaggccat ggcaaagaac
     4681 atcgagagcc tgggccgcga actcgtaccc ctcggttcga ttccacccgg ttttgccggc
     4741 tcgaccgaca tgggcaatgt cagccatcgc gtcccctcca ttcacccgat gctcgcagtc
     4801 gcccctgcga acgtgatcat ccataatgcc gagtttgcgc gctgggcggc atccgaacgg
     4861 ggcgacgcgg cggtgctcga cggcgcgaag tcgcttgcct ggaccgcgct cgagctgaag
     4921 ggcgatcccg cgctgctcga gcggacgcgc gccgacttcg cggcgaccgc cgaaatatcc
     4981 cgctgcgcgc tcgcgcgtct cgaagagaag gtggcagacg cgcaccccgc ggttcacgcc
     5041 catcccggct gcggttgcgc ctgacggatg aaaccctggc tggcggtcgc ggcgatcaac
     5101 ggatttctgg cggtggcggc cggagcgttc ggcgcccatg cactgcaagg ccgcatcgat
     5161 gcgcatgccc ttgccgtttt cgaaaccggc gcgcgctatc agatgtatca tgcgcttgcc
     5221 atcggacttg cggtgctttc gcttcgcggg accccgggtg ccgagctcgc ctgcgctttg
     5281 ttccttctcg gcattgtcct gttctcgggc tcgctgtatg cgctggcgct gagcggcatg
     5341 cgggcattcg gtcttgtgac gcccttgggc ggtctggctt ttctcgccgg ctggggaacg
     5401 ctggcttatt cggctttgaa gacagtttct taggaatcgt ggagatgatc gattttcccg
     5461 cgcccacaat gattcgtacc aatggaatcg acatggccgt gtacgaagca gggccgagag
     5521 acggatttcc ggtcgtgttg tgtcacggct ttccggagct tgcattttcc tggcgccatc
     5581 aattgccggc gctcgccgag gccggctatc gcgccatcgc gcccgaccag cgcggatacg
     5641 gcaagacctc gcgaccggtg gagatgtgcg actacgacat ggcgcatctg accggcgatc
     5701 tcgtcggcct cctcgatgcg ttcggaatcg agaaggcggt gttctgcggt cacgattggg
     5761 gcggcagcgt ggtgtgggca atgccgctct atcatccaga ccgtgtcgcc ggcgtgatcg
     5821 gcgtcaacac gcctttcttc ccgcgtccgc ctgtcgatcc gatcgccctg atgcgcgccc
     5881 gcttcggcga tgacatgtac atcgtcttct tccagaagcc gggcgaggcc gacgcaattc
     5941 tggccgccga tgtcggcaag acgtttcgct ttttcatgcg ccgcaatgtc gcgacggcag
     6001 acgagtacgc gaaacggccg caggcagagc gcaacctcaa gctcgtccgc gcccttcagt
     6061 cggacgagtc cagatggcgc ggcgaggcgc tgctcgacgc acaggaaatg aaagtgttcg
     6121 tcgacatttt cacccgcacg ggcttcaccg gcggcattaa ctggtaccgc aacatcagcc
     6181 ggaactggga actgtcggaa ggcaaggagc cgatcgtcgg cgcgccggcc ctcatgatca
     6241 tggcggagga cgacgtcata ttggcgccgt ccatgaccga aggaatggag cgcttcgtgc
     6301 ccgatctcga gaagattctc cttcgccgct gcggacactg gacccagcag gaaaagcccg
     6361 acgaaacaaa tgacgcgatg atcggctggc tgaaacggcg cttcccctgc tgacgctggc
     6421 cgcgcttttt gccattctgc gtaaatcatg ggcgacaccg cctccgacaa tttttcctac
     6481 gccgagccgg acgatccgcg cctcaagcgc ttcgtgatcc ggctcatcga gcgcatgtcg
     6541 gggcagccct atctcaaatg gctctatgac gatcatctga ccaatccgct tcccggcgag
     6601 agcttctggg atgccgccgt gcgccggctc gagctgaagg tcgtctgcaa cgaggaggtg
     6661 cttcagacgt ggcccaaaga ggggccgctc atcgtcgtct ccaatcaccc cttcggcgtg
     6721 ctcgacggcg tcgtcatctg ccatctcgtg tcgcgggtgc gcggagactt ccgtgtactg
     6781 acgaactcgg ttctggcgcg cgccgagcag gtccgcaaat tcctgctgcc catcgatttc
     6841 accgaatcgc ccgaagcggt gcggacgaat atcgagtcgc gcgccgccgc gaagaagcat
     6901 cttctggaag gcggctgcct gatcgtcttt ccggccggcg gcgtctcgac cacgccgacc
     6961 gtctggcacc gccgcgccgt cgatacggaa tggaagacgt tcaccgcacg gctgatccgc
     7021 caggcgaagg cgcccgtcgc atcggtgttc ttcgccggac agaacagccg catgttccag
     7081 ctcgcgagcc atatcagcac gacgctgcgc ctctcgctgc tgttcaagga agtgcacgac
     7141 aagatcggca gcgaactcca catccgcatc ggcgtgccgg tgccgttcga aaagctcccc
     7201 gccactgccg atcggcagga gttcatgcgt caactgcgcg agacgaccta ccggctcggc
     7261 gaaggcgtgc acccgccgcc aagaccgcgc gtcaagcgcc cgcgccgcgc gccggcccat
     7321 tcccgcaagc cccacgcgaa atgaccgcgg gcgcgcctaa cgacgcgtga ctgacgccaa
     7381 cattttcctc cccgcttgcg ggcaacgggc atctacacat ttcaacgggc gctcctcagg
     7441 ttcctcctcc gcgcgaagcg cgggggagga accgtcaaga tgtatgaata tcgtaggctt
     7501 gcggggggtg gtccgaaggg cctgaggggg agaacagcgg cgcgcgactc actccctccc
     7561 cggccgcagc ttatcctcgg gccggccttc ggccggaccc gggggggcgg acctctcccg
     7621 caagcggggg ggaaaatctc cgagcgacaa cacttgctgc gcgagcttcg acatcagtac
     7681 gtccagcgcc gcgcgttcgc gggggcctag cgcggcgagg aattgttcct cgagcgcgag
     7741 cgcgagcggc acgatctcgc tgtagatggc gcggccgcgt gcgctcagcg acaggcgcgt
     7801 gacgcggcca tccgacatat cggtctgctt ctgcatgcgc ttgccccgca tgaggcgcgc
     7861 gacggcgcgg ctgacctgca ccttgtccat ttccgttcgc acagcgacct cgcgcgccga
     7921 caggcccgac gcctggccga gcgccgccat cacccgccat tccggcgggc tgagatcgaa
     7981 gcgctccgaa tagtgccgcg cgatggcgtt gctgacgcgg ttggtgagca ccgaaagccg
     8041 gtagggcagg aagcggtcga gatcgagcgt ggagcgcgcg cccttccacg cggaacgcga
     8101 ttcattccgc atcgggctgg ttcttcggca gagccgcttc gaaagcggga agcgcaagcg
     8161 cctgctcttc gatgcgcagg agcgtcggat aggcgccgag atcgagatgc acgcgccgcg
     8221 cgttcgccag ctgcgggatc aggcagatgt cggcgagcgt cggcgcatcg ccatggcaga
     8281 atgttcccgt ctgcggcgac tgcgccatca ttgtttcgag cgcggcgaag cccagttcca
     8341 tccaatggcg ttgccaccgc aggcgcgcct cttcgttcgc gcccagccgg tcacgcagat
     8401 agagattcac ccgcagattg ttgagcggat ggatgtcgca gccaaccatg caggccagct
     8461 gccgcacgcg ggcacggccg gccgcgtcgc gcggcaggag cggcggatcg gggcggagct
     8521 cgtcgagata ctcgacgatc gcgagcgact gtccgatcgc gtggccgtca tgcacgagcg
     8581 tgggaacgag ctgctgcgga ttgacggcgc tgtaatccgc accgtgctgc tggccgccgc
     8641 ccttcagcag atgcacgaac ctcaactccg gtttgattcc tttgagattg agcgcgatgc
     8701 gcacgcggaa cgccgcggag gagcggaaat acccgtagag cacgaactca gggccgggca
     8761 cggacgacct cgtgatcgat ggctccgaag atcgaatggc cgcgcgggtc cagcatctcg
     8821 atgcgcaccc ggtcgccgaa atgcaggaac ggcgttgcgg ggcgtcccgt ctccagctgc
     8881 tcgcgcgtgc gcttctccgc gatgcagcag gagccgcgcg aagcgtcgcg attggagacg
     8941 gtgcccgagc cgatgatcga gccggcgccg agcccgcgcg tcttcgccgc atgcgcgatg
     9001 agctgggcga aggagaacgt catgtcgtcg cccgcattgg gggcgccgaa ctgcgcttcg
     9061 ttcagatgca ccgacagcgg cagatggacc ttgccctcgg accacgcttc gcccagctcg
     9121 tccggcgtga cggcgacggg cgagaaggcg ctcgcgggct tcgactggaa gaacccgaag
     9181 cctttcgcca gctccgccgg gatcaggttc cgcaggctca catcattgac cagcatgagc
     9241 agcttgatgt gaggaagagc cgcgtccggc gcggttccca tcggcacgtc gtcggtgatc
     9301 gcggcgactt ccgcttcgaa atcgatgccg aaggattcgt ccgccgcgag gatcggctcg
     9361 cgcgcgccga tgaaaccgtc ggagccgccc tgatacatca gcggatcggt ccagaacgaa
     9421 gccggcattt ccgcgccgcg cgccttgcgc accagctcca catgcgtgac gtaagccgaa
     9481 ccgtcggccc attgataggc gcgcggcagc ggcgccgcgc agtcttcgga cgcaaatggt
     9541 ctggcgccat cggcggcgcc ttcgttcaac agatcataca cgcgcatcag ccggggcgcc
     9601 gcgtgatgcc agtcgtccat cgcctcctgc agggtcgagg cgatgtgggg caccgcgaca
     9661 cagcgcgcga gatcgcgatc ggccaccacc agcaggccgt cgcggccatg gcgaagcgac
     9721 gcgagcttca tgtccgccgc gaactttccg gcttttcccc ccagctgtcg gcataggcgc
     9781 gatcttcatg cgcctcggcc tgtggcgcca gctcgaacgg atcgcgcgaa tcgatcatca
     9841 ccgcgacctc gtctgtctcc ttgcgcgcgg ccctggcgcc cgccgcaaac gccctgggat
     9901 gcgggccatg ggtgatgccg ccgggatgca cggtcatcat tcccggatgg atgttgtcgc
     9961 ggctgaagaa ttcgccgcga tggtagaaga tcgcctcgtc gtaatcgtcg ttggaatgga
    10021 agaatggaac cttgagcgcg ccgggatcgg tctcgatcgg ccgcggcacg aaggtgcaga
    10081 cgacgaagcg gtcggcgacg aaggtggaat gcacggatgg agggacatga taacggtggc
    10141 tcatcaccgg cctgatgtcg cgccaattga gcctgacggg cgcgaggttt cccttccagc
    10201 ccgccgcgtc gagcggattg aacggataga cgatggtgct cacctgtttg cggcgcttga
    10261 cgtcgacgcg ccactcgccg tcggcctgtg cggcaaaggc ttcgtcgagc ctgggcgttt
    10321 ccagcgccgc cgggtcgaag atcgcgtgcg gccccagaag cccgcgctcc gagagtttat
    10381 aagcgccgtc caccgcctcg atcgtcaata tgaccgcccg cggcttcggt tcgatgcgcc
    10441 acatcgtgcc gcgcggcaac aggatgtagt cgccgtcgtg ccagccgaga tggccgaaat
    10501 cgcaaaagag atcgcccgca ccttcgtgga tgaagagcag cgtgtcgccg tcggcgttgc
    10561 gcgccaggcg ccgcatcggt gcggcgcacg tccacatcgc gacggcgcat tgctggctcg
    10621 caagaatcgg ggcggcgccg aacggtgtgc tggagacctc ggccgccttg gcggcatcga
    10681 aggcgcgcgg acgcaacgcc ccctcgatcg atgtccagcc ggtcggcgca tggcgatgat
    10741 aaaaatgcgt cgcggggccg aagaatccct cgcggcccat ttcgcgttcg aacgtgccgt
    10801 ccggaagatc ggcatgggcc tggcgcgagg tgacgccttc gacgcgcgga aactcgatcc
    10861 agtttttcgc gctcatgcct cgaaaactcc tctgcggatc tgatcggctt cgatcgattc
    10921 gaacagcgcc tggaaattcc cctcgccgaa accctcattg cccttgcgct ggatgatctc
    10981 gaagaagatc gggccgatgg cgttctgggt gaagatctgc aggagcaggc cctgatcctc
    11041 ggtgggcgcg ccgtcgagca ggatgcgctc gcgcttcagc cgctccacat cctcgccatg
    11101 acctttgacg cggttgtcga cgcgctcgta ataggtgtcg ggcgtgtcct ggaaggcgac
    11161 gccgcgcccg cgcagctttg ccaccgagcc gtagatatcc tcggtcgcaa gcgcgatgtg
    11221 ctggatgcct tcgcccttgt agagatgcag atattcctcg atctgcgact tgtcatcgga
    11281 ggattcgttg atgggaatgc ggatcgtgcc gcaggggctg gtcattgcgc gcgacttcag
    11341 gcccgtcagc ttgccctcga tgtcgaaata gcggacctcg cggaaattga agagcttctc
    11401 gtagaagccg gcccagcggg tgagattgcc gcggaagaca ttgtgggtga cgtggtcgat
    11461 cgccgcgagg ccggcgccgt catgcgcaaa tcctttcgcc gcgtcggtgg ccacgaagtc
    11521 cacatcgtag atcgagcgat cgccgtagcg gtcgacgaga tagatgacgc tgccgccgat
    11581 gccctcgatc gcgggaatgt tgagctccat cgggccgacc tggttgtgga acggcacggc
    11641 gcccagttcg agggcgcgcg cataggcttt gcccgcatcg tgcacgcgga acgccatcgc
    11701 gcaggcggac ggtccgtgcg ccctggcgaa acggctgcca tggctgtccg gctccgcatt
    11761 gacgacgaaa ttgattccgc cctggcggta aagcgtcacc gccttggagc ggtgccgcgc
    11821 cacggcgcgg aattccatgc gttcgaacag cgcgtgcagt gcgcctgtat cgggcgcggt
    11881 gtactcgacg aattcgaacc cgtcggttcc catgggattg tcccacaatg ccgccatcac
    11941 gctctccttc ccgccatgtc ccggattagt ttcaaatgaa accagttgcc tggtcaagcg
    12001 gatttgccgg cgttgctcgt tttgctttcc tcccccatcg tgggggaggt ggtttgcgta
    12061 gcgaagcgga gcaaaccgga ggggggagtc cgcgcgcggc gcgcgcgcca ctcccccctc
    12121 cgcctcgcgg agtttatcct tcggccgcgc ttcgcgcgga ccgggggctc ggcacctccc
    12181 ccacgatggg ggaggaaaat ggagggcatg ttcggcatcg aatggaacat gaaacgatgt
    12241 gtaccgacgc tcccccgcgt tgggggagga aaaggagctg cacattttgg tttcttatgt
    12301 cggtggcgcg ctttagtctg gcggcacgcg agggggatga gatgacgaac aggattcctg
    12361 tcggacagac gatttccggc gcgtataatt ttgcgttcac gcgctttcta tcgatcctgg
    12421 cgatcgcttg gctgccctac gccgtcttcg cggtgattgc agtggggctt gccctgcttg
    12481 cggtgccgga tctcccgcgc atgttgacga cgcacgatat cgacatcgag gcaatgatgg
    12541 gcctgagccg cgtcgtgctg ctcatcgccg tcatcggctt catcatgtcg tccatgatca
    12601 cggtcggcct gcagcgccag gcgctcggaa agcaaaccgc gcccgtctgg tactatttct
    12661 cgctgggcgc tccggtctgg cgcatggccg gcgcgttctt ccttgccggg ctcgtcatcg
    12721 tcttcatcgc catcgtgatt tccgcggcct gcgccgcgat ctggttcgcc gcgggaacca
    12781 tcccgggcgc ggtgtggcca atccgcgtcg tcgccattgc ggcagggacc tgtttcctca
    12841 tttacatcgc gacccggctg ttgttcttcc tgcccgccgt cgtgactgcg gaagagtcga
    12901 tcggaatcga acgcgcctgg attctcggcc gcggcaattt ctggcgaatc ttcctggtct
    12961 ggcttgcggt gattgttccg gtcgcgatcg cattctggat cgtgtcgcgc gccgttcttg
    13021 gcccattcgt gccgtggccc catgcccata tgggcacgcg cgagatgata cgcatgttcg
    13081 taacgcaatc cagtgccttt gcaccgctgg tcctggtggt cgaattcgtg gagcgcgtcg
    13141 tgatcctggg cgtcgccaat ggagcggtcg ccaatgccta tctcgcggta gcgggcgcgc
    13201 cgccggtcgc gccggccgcc gcgcgacagg ggtccgagcc cggctttggc tccggcaccg
    13261 gagcctgata agagcagggc agcgaatctc ccgaggcccg tctccaaggc gcacatgatc
    13321 aggattcccg tcgcggagac gatccgcttc gcttacgcgt tcgccttcgg acagatcggc
    13381 gccatcatcg gactggtgtg gctgccgttg ctcgttgcgg cactgctgca gttccttccc
    13441 tatgcgatcg gtaccgccta tccgcccgac gcttccgatg cggcgggagc aacgggcgcg
    13501 gcgctgctca acctcgcttt cgtggccgcg acgctcgtcc tctatgcgat gaactgcgtc
    13561 tcggtgacgc ggcaggcgtt gggtctgcgc cagggtgcgg cgacagttca tttttccctc
    13621 gcccggccgg aatggcgcat gttcgctgcc atcctcgttt gcggattgat cactgtttcg
    13681 gtgctcatgt tttgccttct cgtcgcgaca atgcccgggc gccttgccgg agcgccgggg
    13741 ctgctggagg tggtcgccgc gcttgccggg cttgccgccg ccgtctgggt cacgctgcgc
    13801 ctcatatttt ttgtgccgcc ggtcgtcgtg gcagaggagc ggatcgatct tccgcgcgcc
    13861 tggatgctgt cgaggcagaa cttctggcgc attctggcga ttgtgctggc ggcaaccgtt
    13921 cccttcctgg tgatacaggg cgtcgtgttc ctgatcctcc tcggtcccgg attcgtcgcg
    13981 ccgctgccca ccaacaccgc cgccgcggcc gtcgcactca cgcgccgcct cgcgctgttc
    14041 gaccgccaca tgccgacggt gatcggattt gcactggtgc tcgcaccctt caacctcgga
    14101 ttgacattgg gcgcctcggc cttcgcctat cgcgcactcg tccccgcccg cccctccgtc
    14161 cgcagcgccg gctaagcgcc aaggcgcgcg tttgcggtct ccgcaaagaa aaattcacag
    14221 ggaggcagtg aggaagtgag aatcgttgcg cgcctttggc gcgctattct tcctcgccgt
    14281 ctcccacccg gttaaaccgg gtggctcctt gtgaatcttc gtcatctgcg gcgggctccg
    14341 ctaccggcgg cgggcggcga ggccggcgag ggcgagatcg agaatggcat ccgtgtcgcg
    14401 gctggtgaga ggcgcgtgcc cgatcagcag gcggaaatag aatggcgcgt agatgaggtc
    14461 gagcgccgcc tcgatatcga ttttgcggcg gatgccgccc tcggcggcgg cgcgcgccag
    14521 caacgcgcgg ccctcctcgc ggctcttcat cacgaaatgg gtgcggaaca cctttgcaag
    14581 ttcgctgtcg ttctgcgcgg cggcgatcat catcgcggtg ctgcggcccg cgcgcgtcgc
    14641 cagggtttcc gccagttcgc gcaaccggga gcggagcgcg gccaatgcgc tgcgggattt
    14701 gtgcgatggc ggagcagtcc ggtcgcgctc gagaaaggcg ctcatcgcca ccgcgtgcgc
    14761 attgggccat ccgcggtaga tcgtgggcct gccgacgccg gcaagcgccg cgatcgcttc
    14821 catcgtcacc gcgccgagcc cgccttcgtc gagcagtttc gccgccgccg ccaggatgcg
    14881 cctgcgggtt cccggatcgc gaggccggcc cctcgcgttt tttttcggtt gcgccatgct
    14941 tgactgcgtc ccatattatc gttacgatac gtaacgtaat agatcgcaat tcgcggtcag
    15001 gtgcaagagg agcatggatc atgagtgcaa cctccccaac ccgcggttcg cccgtccgcg
    15061 gctccataac gccctacatc atcgtcaagg gcgcagacgc ggcgatcgcc ttttatacga
    15121 aggttttcgg cgccacggag gaatcgcggc tttccgaccc cgacggccgt atcggtcatg
    15181 cggagctcgc catcggcgac gggcatctga tgctcgccga tgaaacggcc gccttcggcg
    15241 cgctcagtcc tcccgccatc ggcggcacac ccgtcaagct tcatctccct gtcgacgacg
    15301 tcgatgcggt gatggagcgg gcgcgccagg ccggtgcaac gatcctgcgc ccggcgcagg
    15361 atcagttcta tggcgagcgg tcgggaatgc tggcagatcc gttcggacac cagtggttcc
    15421 tcggcacgcg caaagaggcg ataagtccgg aggagatgca gaagcgctac acggattcgt
    15481 tcaaggtcgc gtagcaccgg gaaattgaag gaccgccatg cgatcgcgca aggccggcat
    15541 cgcgctcgta ctcgccgtcg tctatgtcga catgctgggc atcggattgg cgtttccgat
    15601 tctgccgcgc ctcgttcaac aattcgagca tggcgacgtt tcgcgcgcag cctggatcta
    15661 cggcctgctc gcggcagcct acgcactgat gcagttcatg ctcgcgccaa tgcttggcgc
    15721 attgtcggat cgcttcgggc gcaggcctgt cctcctgctc tcgctcctgg gcatgggcct
    15781 gaactacgcc gtgcttgcgg ccgcgccttc gctcgcctgg ctcgctgcag ggcgcatcgt
    15841 ggctggtgcg atgggcgcca gctattccac ggcgagcgct tatctcgccg acatcacgcc
    15901 gccggaaaaa cgcgcgcaga gcttcgggct catcggcgcg gcgttcggtt tcggatttat
    15961 caccgggccc gcgctcggcg gcttgctggg cgacatcgat ctgcggctgc cgtttgtcgt
    16021 cgcgtcagtg ctgagtttcg cgaatctggc tctcggctat ttcatcctgc cggaatcact
    16081 tccgccggaa catcgcaagg acttcagcct ggcgcgcgcc aatccggtgg gcgcacttgg
    16141 cacgatcggc cgctaccccg ccatcggcgg ccttcttctt gtcttcgttc ttgcgacctt
    16201 cgccaataaa gttgcggaga cgacatgggt gctgttcgca acctacaaat tccactggaa
    16261 tgccgcttcc gtcggtcttt cgctcgccat ggtgggcgtc atattcgtga tcgggcaggg
    16321 cgggctggtg cgtgtcgtgg tgcccaggct cggcgagcgc cgctcgattc tgctgggcct
    16381 gttcgtgaac gctgtcgtct tcctgctgta tggcggcgtg cccgaaggct ggatggttta
    16441 tccggcgatg gtgcttggcc tgttcggctg gaccattgcg caacctgcca gcatgggcgt
    16501 gatgtcgcgc gccctgccgg caaacgagca aggactgctg caaggcggca ttgcgagcgt
    16561 caacagcctg gccgccgtga ttggtccacc cgtatggact tccatcttcg cttatttcgt
    16621 cggtccggcc acgtctgttg ttatacccgg cgccgcattc gatggcgcgg cgctcgtctt
    16681 cttcatcgcg cttgtgctcg cgatccgaac gttgccggcg gaaagtatgg cagacgtggc
    16741 gcttgccaac acggggacat gatactgatc tctggaatcg gggacgatac gggcgcccgc
    16801 cggcccgcgc ccggcgcgcc cggcgcccgg ggtgggaagc cgcgggacga tgggagctcg
    16861 aacattgcat gaacagaatc attacagttt tatttaccat gacggatcac aatcctgtcg
    16921 ttttcgattc gttcttgtgc aacgcaagca ctggaagcga gcggtccacc cgtagtcttc
    16981 gccaactgcc cggttttctt gggtttttgc ttgactcgac cctttatccc atgttatccc
    17041 atgccgtccc atgctgatag gccgtacagc cccgtgaatt gaccggctgc ccgacccggc
    17101 aagggatccg cgagggggca gtcaatccac atgggcgtgg cagcgcatcc gccgttcatc
    17161 tcgatgcccg tcggcgcgct cgacaggaag ggccgcgtct gcattcccgc gtcctaccgc
    17221 cagattctca ccgcccagag cacgaacggc gtctatgtct gcccgtcgtt ctacgagccc
    17281 gcactcgaat gcttcggcga gaacgtcctg caggagttcc accagcagca ggccggcgat
    17341 gacccgttct tcgccgaaac ccatgacgtg aaggcgttct cggtgctcgc gatgacacag
    17401 ctgcttgcgc tcgacgaaac gggtcgcgtg cgccttcccg atgcgctgat cgcccatgcc
    17461 gggcttgtgg aaaacgtcac gttcgtgggc atggggcgca aattcgagat ttgggacacg
    17521 cagcgtttcg cgccggtgct taacgagcgg ctcgccagcg cgcgcgcgct gcgcaaatcg
    17581 cagccggtgg cgtcatgaac cagggccacg cgccagtcct gctcgcagag gtgctcgacg
    17641 cgctcgcgcc gcgcgatggc gcccattacg tcgacggcac tttcggcggc ggcggttatg
    17701 cgcaggccat tctcgatcgg gcaaaatgca aggttctcgg catcgaccgc gatccggacg
    17761 cgatcatacg cggcgcggcg ctcaaggagc gttacgcagg gcgtctggct cttgcgcagg
    17821 ggcgcttttc cgcaatggag cagctgctgg cggagcagca cgaaacggga agcgacggag
    17881 tcgtgctcga tctcggcgtc tcgtctttcc agctcgacga gcccgggcgc gggttctcgt
    17941 ttcgtggcga cggcccgctc gacatgcgca tggaacggca aggttcgtcc gcgggcgatt
    18001 ttgtcaacac cgccggcgag gcagagcttg cggacgtgat cgcccgattg ggcgaggaac
    18061 ggcaggcgcg gcggatcgcc cgcgcgatcg tcgccgcgcg cccgctttcg cgcacgggcg
    18121 aactggcgga aattgtcgtc gaggcgctgg gcccgggagc ggcgcgccac aagatccatc
    18181 cggccacacg gacctttcag gcgctacgcc tgcacgtcaa caacgagttg ggcgaactgg
    18241 cctcgggcct cgccgccgcc gaacgcgtgc tgagaccaag cgggcgtctg gctgtcgtat
    18301 cgttccattc gctcgaagac cgtatcgtca agcagttcct cgtggaacgc agcggtcgcg
    18361 cgccggtcgg ttcccggcac gctcccgcgt cacctgctgc gcgcgcgccg acattccggc
    18421 ttcttccgcc gcaaccgcgc atgtccagcg ccgcggaaat cgcgcgcaat ccccgttccc
    18481 gctcggcgcg cctgagggcg gccgaacgca catccgtccc gctcgcggcc taggaggcaa
    18541 tgatgatacg gattctgaac ttcttctgtt ttgcggccgc cgccttgtgc tgcctcgcgc
    18601 tttaccacat ctcggaacag acgcgcgttt cgcgcatcca cctgctggcg gtggaaagac
    18661 aaatcgccga cgagcacggc gcgatgaagg ttctccaggc tgattgggag cgcgtctccg
    18721 agccgtcgca catccaggcg ctggcgcaga ccaaactcgg cctctcggac gtgccggcag
    18781 tcgcgctggc gtcgctggag cttctgccgc gccggggcga gactccgctg gccggctcgc
    18841 ccgtccagac cgccagtatc accgttggga caatttcgtc cgattcgcat cttcgccttg
    18901 cagccgccca tgcggggaac tgacggacaa atctacgctc cagtcgagcg caggcaggat
    18961 ggacgcgaag ccgtttccgt gatccagcgg cgcattgtcg tcgcggccgg tatctgcgtg
    19021 atggcctttg cattgatcgg cgtcaggctg gtcgatgtcg gcatcatgaa aggcagcgtt
    19081 accggcgcat cggcgccgat cggcgagcag ccgcaacccg cgcgcgccga tattctcgac
    19141 cgcaacggtg ttgtgctcgc ccgcgatctg cctgtcggcg atctctatgc acggccgcac
    19201 gtgtttgccg accggcgcga agcggcacgg cagcttgccg ccctggccgg cgtcagctcc
    19261 gagcgcctgg cgcgtgcctt cgacagccgg cataattacg tcctcgtcgc gcgtcagctc
    19321 gttcccgacg tgcaggacaa gatccgccgg ctcggtctgc ccgggctcga attcgaaccg
    19381 gcagcgaaac gctactatcc cgaaggcctg gcggcggtgc aggtgctggg cacgaccgat
    19441 ccggacggca acggcgtgga tggcctcgag cttggcctcg attcgacgct cggccaggcg
    19501 gagcccggcg cgggcattgc tgtttcgctc gacatgcgcg tgcagtatgc gctggctcat
    19561 gaagtcgagc aggcgcggga aaccttcggc gcgcgcgcgg cgggcggcat cgttctcaat
    19621 gtgaacaccg gcgaaattct cgccatggcc tctctgccgg atgctccggg cgagacgggt
    19681 gcggctgcaa acgccgatcc gcgccgcaac cgcatggcgc aggatgtcta cgagctgggc
    19741 tccgtcttca agattttttc ttttgcgctc gcgatggagg atcacaccat ccgtctggac
    19801 gagatgtttc cgatcgcggc gggctttcac ataggccgct acacgatcca cgacgccgag
    19861 catatgccgg cgacgctcgc cgcgcgcgac atccttgcgc aatcgtccaa tgtcggcacc
    19921 gcgcagatcg ccttgcgttc cggcccggag cggcagcgcg cctttcttgc ccatcttggc
    19981 cttctcgcac ccgtgcggac cgagttgccg gagcgtgcgg cccctctggt tccccgccat
    20041 tggagcgaag tcgagaccgc caccatcgga tttggccacg gcatctccgt ctcgcccttg
    20101 tcctttgtta ctgctgcagc tgccctggta aacggcggcc ggcgaatcgt gcctaccttt
    20161 ctcaaacagg catccgacgc gcgcggggag cagctcatca agcccgagac cagcgccacg
    20221 atgcgcgagc tgctgcgtta cgtcgtgacg aacggcacgg gcaaaaaggc ggacgtcccc
    20281 ggctacgata tcggcggcaa gaccggttcg gctgaaaagg tttccggacg cggctatgtc
    20341 gcccatcgtc tgctcacctc cttctgcgcc gtatttccga ttgaaaatcc gcgctatctc
    20401 gtgttcgtca tgctcgacga gccgcatggc acgaaggcaa ccttcggcct tgctctggca
    20461 ggcttcactg ccgcaccgct cgccggacgc gtcgtcgcgc acattgcgcc gatgctgggc
    20521 atgccgacgg acgcctccgc cgtggtgcag gccaaagaca actcataatg gaaacacaat
    20581 cgggggcgat cgtagcagcg ggaatggcga gcatcgcgcc catgcaggag ttcagcgaat
    20641 ttgcgggtct ggcgagcgac agccgctcgg ttggacccgg ctatcttttt gcggccttgc
    20701 ctggcacgca ggccgacgga gcgctgttca tcgccgatgc ggtacggcgt ggcgcgatcg
    20761 cggttctcgg ccggccttcc gttcgccccg aagccgaaaa gcacggcgtg ccgctcattg
    20821 ccgacgacaa tccgcgtctg aagctggcaa aaatcgcttc cgaacatttc ggcgcccagc
    20881 cgcg
//
LOCUS       DASZMC010000571         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1632683,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000571 DASZMC010000000
VERSION     DASZMC010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632683"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VG274_11665"
     CDS             complement(<1..1096)
                     /locus_tag="VG274_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWE07360.1"
                     /translation="MSRVDRRGAADWWWTVDRTALVAMLALFAIGLMLAFAASPAATG
                     GPMTEGDFRYAAKQIAFAGLAVVILGATSVLPLRTIKLSAALIYAAAIAGCVVALFAG
                     NEVLGARRWIDFGWVTLQPSEFLKPSFAVLAAAVLADNEKMILPKEVVAFILAAPALL
                     ILVLQPDVGQTGLLLALWGALLFFNGVSLAAVGILAGSSAGLGFLAYLLFPHVHHRIN
                     QFFDPQSAGYQTGLALKAFAHGGFAGVGPGAGTVKYHLPDAHSDFVFAVAGEEFGIAL
                     CGIIVLLFCVLCIRLLLRSARMADAFSGLAGAALAIAIALQAFINMGVAVSLLPTKGM
                     TLPFISYGGSSLFAVALTMGFALAVTRQRSK"
     gene            complement(1122..2540)
                     /gene="murD"
                     /locus_tag="VG274_11670"
     CDS             complement(1122..2540)
                     /gene="murD"
                     /locus_tag="VG274_11670"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWE07361.1"
                     /translation="MIPVRSFSNRDIGVFGLARSGISAVQALKAGGARVFAWDESEQP
                     RSDAAAQGVHVAPFAAWPWSRINTLVLSPGVALTHPSPHQVVVAARGAGAEIIGDIEL
                     FAREIRPDAARAGRAPVIAVTGTNGKSTTVALVGHILSTCGFQAETGGNIGRPVLDLA
                     PPSARVIYVLEISSYQIDLAPGLVPDVAVLTNISPDHLERHGSLANYAAVKARLLEQA
                     SSDGCVVIGVDDANSAAIHTRLSARRATSAVPVSVGKVLGRGIFAVDGALYDAWGNRA
                     SNVMELGVADRLPGAHNWQNAALAYAAVRPFVKDSRAIASAIAGFPGLAHRIEDVGRV
                     GKVRFINDSKATNADAAARALACFPDIFWIAGGRPKEGGIDSLLQYAPRVRKAYLIGE
                     AAEMFARALSGHAPHEIRGTLEDAIAAAFADASRSGVAAPVVLLSPACASFDQFRDFE
                     ERGDRFRVLAGELAGTARREAS"
     gene            complement(2551..>3480)
                     /gene="mraY"
                     /locus_tag="VG274_11675"
     CDS             complement(2551..>3480)
                     /gene="mraY"
                     /locus_tag="VG274_11675"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWE07362.1"
                     /translation="KGQPIRTDGPPRHLIEKQGTPTMGGLLILISSVAATLLWADLTQ
                     AYVWIVLFVTLSFGLLGLVDDYLKVTKRSPEGVSANTKLLIQVAVAVLASFAAMSLEE
                     QSLAGTVAIPFLKTVLLHLGWGFPVFGALVIVGASNAVNLTDGLDGLAIVPVMIAALA
                     FALIAYLVGNVKFADYLQLHYIAGTGELAVFLAALVGGGLGFLWYNAPPAMVFMGDTG
                     SLSLGGALGGVAVATKHEIVLAIIGGLFVIEAVSVIAQVASFKLTGKRIFRMAPIHHH
                     FEQLGWQESTIVIRFWIVSVVLALIGLSTLKLR"
BASE COUNT          728 a         1107 c         1114 g          531 t
ORIGIN      
        1 tcttcgatct ctggcgcgtg acggcgaggg caaaacccat cgtcagggcg acggcgaaca
       61 gcgacgaacc gccataggag atgaaaggca gcgtcatgcc cttggtgggc agaaggctga
      121 cggcgactcc catgttgatg aatgcctgca gcgcaatggc gatcgcgagc gcggctccgg
      181 cgagacccga gaacgcgtct gccatccgtg cagatcgcaa cagcagcctg atgcagagca
      241 cacaaaacag cagcacgatg attccgcaca gcgcgatgcc gaattcctcg cccgccaccg
      301 cgaagacgaa atccgagtgc gcgtcgggca ggtgatattt gactgttccg gctccgggtc
      361 cgacacccgc aaatccgcca tgggcgaacg ccttgagtgc caggcccgtt tgatagccgg
      421 cggactgcgg atcgaagaac tggttgatcc ggtgatgcac gtgcggaaac agcagatagg
      481 cgagaaagcc caggccggcg ctcgatccgg cgagaattcc cacggcggcc agcgaaacgc
      541 cgttgaagaa caagagcgcg ccccatagcg ccagcaacag cccggtttgc ccaacgtcgg
      601 gctggagcac caggatcaac agcgccggtg cggcaagaat gaatgcgacc acttccttcg
      661 gcagaatcat tttctcgtta tcggcaagca cggccgcagc aagaacggca aaactcggtt
      721 tgagaaattc cgaaggctgc aaggtgaccc agccgaaatc gatccagcgc cgcgcgccaa
      781 ggacctcgtt gccggcgaaa agcgcgacca cgcagccggc aatggcggca gcgtaaatca
      841 atgcggcgga cagtttgatc gtgcgcaacg gcaataccga cgtcgcgccc agaatcacga
      901 cggcgagtcc cgcgaacgcg atctgtttcg cggcatagcg gaaatcgcct tccgtcatcg
      961 gcccgccggt ggccgcggga ctcgccgcga atgccagcat caggccgatc gcaaacagcg
     1021 caagcatggc gacgagcgcc gtgcggtcca ctgtccacca ccaatcggcg gcgccgcgtc
     1081 ggtcgacccg gctcattggg cgcacccgtt tgcggcgacc ctcacgacgc ttctctccgt
     1141 gcagtcccgg caagctcgcc ggccaggacg cgaaagcggt cgccacgctc ctcgaagtcg
     1201 cggaactgat cgaaggaggc gcatgccggc gaaagcaaaa cgacaggcgc ggcaactccc
     1261 gatcgcgacg cgtcggcgaa cgccgcggca atggcgtctt cgagcgtccc gcggatttcg
     1321 tgtggcgcat gaccgctcag tgcgcgcgcg aacatttcgg cggcctcgcc aatcagatag
     1381 gctttgcgca cccgcggcgc gtattgcaga agcgagtcga ttcccccttc cttgggccgg
     1441 ccgccggcga tccagaatat gtctggaaaa catgccagcg cccgcgcggc ggcgtcggcg
     1501 ttcgtcgcct tggaatcgtt gataaagcga acctttccca cgcggccgac atcttcgatg
     1561 cggtgagcaa ggcctggaaa accggcaatc gccgaagcga tggcgcgcga atccttcacg
     1621 aagggccgca cagcggcgta ggcgagcgcg gcattctgcc aattgtgcgc gccgggaaga
     1681 cggtcggcga cgccaagctc catgacgttc gacgcgcggt ttccccaggc gtcgtagagt
     1741 gcgccgtcca ccgcgaatat cccgcgccca agcaccttgc cgaccgacac gggcacggcg
     1801 ctggtggcgc gcctggccga cagccgcgtg tgaatggcgg cgctgttggc atcgtcgacg
     1861 ccaatcacga cgcagccgtc ggacgacgcc tgctccagga gccgcgcctt gacggctgca
     1921 taattggcaa gcgacccatg ccgttcgagg tgatcgggcg agatgttggt cagcaccgca
     1981 acgtcgggaa cgagaccggg cgcgaggtcg atctggtagg acgagatttc cagaacatag
     2041 atcacgcgcg cgctcggggg cgccagatcg agcacgggcc tgccgatatt tccgcctgtc
     2101 tcggcctgga agccgcaggt ggagagaatg tgcccgacaa gggccaccgt cgtcgatttg
     2161 ccgttcgttc ccgtgaccgc gatcaccggc gcgcggccgg cgcgcgcagc atcggggcga
     2221 atttcgcggg cgaaaagctc gatgtcgccg atgatttccg cgcccgcgcc tcgcgccgca
     2281 actaccacct ggtgtgggct tgggtgcgtc agcgcgacac cggggctgag cacgagtgtg
     2341 ttgatgcggc tccacggcca cgcagcaaat ggtgcgacat ggacgccttg cgccgccgca
     2401 tcgctgcgcg gctgttcgct ttcatcccac gcgaaaaccc gcgcgccgcc ggctttcaaa
     2461 gcctggacgg cggaaatgcc cgatcgtgca agcccgaaca cgccgatgtc gcgattgctg
     2521 aaggaacgga cgggaatcat ggcccgttcc tcaccgcaac ttcaacgtcg aaaggccgat
     2581 cagcgccaga accaccgaga cgatccagaa gcggatcacg atcgtggatt cctgccagcc
     2641 gagctgttcg aaatgatgat ggatgggcgc catccggaat attcgtttgc ccgtcaactt
     2701 gaacgacgcc acctgggcga tcacggagac cgcttcgatg acgaacaatc cgccgatgat
     2761 tgccaggacg atttcgtgct tggtggcgac cgcgacgccg cccagcgctc cgcccagcga
     2821 aagcgatccc gtgtcgccca tgaagaccat ggccggcggc gcgttgtacc agaggaatcc
     2881 gagcccacca ccgacaagcg ccgcgagaaa cacggcaagc tcgccggtgc cggcgatgta
     2941 atgcagctgc agataatcgg cgaactttac gttgccgacg agataggcaa tgagcgcgaa
     3001 tgcgagcgcg gcaatcatca ccggtacaat ggcgagcccg tcgaggccgt cagtgagatt
     3061 gaccgcgttc gacgcgccca cgatcacaag agcgccgaag acgggaaagc cccagccgag
     3121 atgcagaaga acggttttga ggaacggaat ggccacggtc ccggccagcg attgctcctc
     3181 gagtgacatt gcagcaaatg atgccagaac cgcgacggcg acctggatca gcaatttggt
     3241 attggcgctc accccttcgg gtgaacgctt tgtgaccttg agataatcgt ccaccaaacc
     3301 aagcaggccg aaactgagcg tgacgaacag gacgatccag acataggctt gggtgaggtc
     3361 cgcccacagg agcgtggccg cgacggaaga aatcaggatc agcaggccgc ccatggtggg
     3421 cgtgccctgt ttctcgatga ggtggcgagg cggtccgtcg gtgcggatcg gctgaccctt
//
LOCUS       DASZMC010000572         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_235049,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000572 DASZMC010000000
VERSION     DASZMC010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235049"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /gene="ltrA"
                     /locus_tag="VG274_11680"
     CDS             <1..1401
                     /gene="ltrA"
                     /locus_tag="VG274_11680"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWE07363.1"
                     /translation="HRTQIRARVTQALDRVRQAARQRKKERFTALLHHVNIDSLRVSF
                     YALKRKAAPGVDGVTWDDYEADLELRLEDLHGRVHRGAYRPQPARRTYIPKADGKQRP
                     LAIAALEDKIVQGATVMVLNAIYEADFLGFSYGFRPGRGPHDALDALAVGIADRKVNW
                     ILDADIRSFFDSVSQEWLIRFVEHRVGDKRIVRLIRKWLRAGVLEDGVVSVGDRGTGQ
                     GSVISPLLANIYLHYVLDLWAERWRRREATGDMIIVRYADDLVIGFEHEADANRFLDA
                     MRARFAEFSLSLHPDKTRLIAFGRHAAADRARRGQGKPETFNFLGFTFICGKSRAGKF
                     LIHRKSRRDRVQAKLKEIKKELRRRMHGPIREQAEWLKQVVSGYFQYHAVPTNVRALV
                     AFRYHVTDLWRRTLRRRGQKHRMTWGQIGKLADHWLPKPQILHPWPNQRFAVKHPRWE
                     PYAGKPHVRFCAGGAQ"
BASE COUNT          302 a          519 c          511 g          239 t
ORIGIN      
        1 caccggacgc agatccgggc tcgcgtgacc caggcactgg accgcgtacg gcaagcagca
       61 aggcaaagga agaaggaacg gttcaccgcg ctcctccacc acgtcaacat cgattcgcta
      121 cgggtgtcgt tttacgcgct gaagcgcaag gcggccccag gcgtcgatgg cgtgacatgg
      181 gatgactacg aggcagacct cgagctccgg ctcgaggatt tgcacggtcg ggtccatcgc
      241 ggagcgtatc ggccgcagcc cgcccgccgg acgtacatac cgaaggcgga cggcaagcaa
      301 cggccgttgg cgattgccgc cctggaggac aaaatcgtcc agggcgccac cgtcatggtg
      361 ctgaacgcga tctacgaggc ggacttcctc gggttctcgt acgggttccg gcccggacgg
      421 ggaccgcacg acgcgctcga cgccctggcg gtggggatcg ccgaccggaa ggtgaactgg
      481 atcctggacg ccgacatccg ctcgttcttc gattcggtga gccaggagtg gctcatccgc
      541 ttcgtcgagc atcgggtcgg cgacaagcgc atcgtccgcc tgatccgcaa atggctccgg
      601 gcgggagtgc tcgaagacgg ggtcgtgagc gtcggcgaca gggggacggg gcaaggctcg
      661 gtgatctcac cgctgctcgc caacatctac ctgcactacg tcctcgatct ctgggccgaa
      721 cgctggcgac ggcgcgaggc caccggcgac atgatcatcg tgcgctatgc cgatgacctt
      781 gtgatcggct tcgagcatga ggccgacgca aatcgattcc tcgatgcgat gcgcgcgcgg
      841 ttcgcggagt tctcgctgtc gctccatccg gacaagaccc gcctgatcgc gttcggccgc
      901 catgcggcgg cagatcgcgc gcggcgcggg cagggcaagc cggagacctt caacttcctg
      961 ggcttcacct tcatctgcgg caagtcccgc gccggcaagt tcctgatcca caggaaaagc
     1021 cgacgcgacc gcgtgcaggc gaaactcaag gaaatcaaaa aagagttgcg acgccggatg
     1081 cacgggccga tccgcgaaca ggcggaatgg ctcaagcagg tggtcagcgg ctatttccaa
     1141 taccacgcgg tgccgaccaa cgtccgcgcc ctcgtggcct tccgctacca tgtcaccgac
     1201 ctctggcggc gcacgctccg gcggcgcggc cagaaacatc gaatgacctg ggggcagatc
     1261 gggaagctcg cagaccactg gctccccaaa ccccagatcc tccacccctg gccgaaccaa
     1321 cgcttcgccg tcaaacaccc aaggtgggag ccgtatgcgg gaaagccgca cgtacggttc
     1381 tgtgcggggg gcgctcagta atgagcgtcc ctaccgcaat ccgaatcccc caccatcacg
     1441 cccgacgggc aggtgacgca gctccgcatg ccggaagagc agtatgtgac caatatcgcg
     1501 ttcggccgcc atgcggcggc agatcgcgcg cggcgcgggc agggcaagcc ggagaccttc
     1561 aacttcctgg g
//
LOCUS       DASZMC010000573         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_802227,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000573 DASZMC010000000
VERSION     DASZMC010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802227"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VG274_11685"
     CDS             complement(<1..842)
                     /locus_tag="VG274_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HWE07364.1"
                     /translation="MVPLSLTSRASFARLGPHKGGGNPSIDASLTNNELPSVVVAKDH
                     NAILLHALDEAAVRAGLSIGLPLANARAICPELTVYDADPAADLKTLNDIADWCDRFT
                     PLVALDPPYGLFLDITGCAHLFGGERALLQIVTGALTRRGFAVSAAIAGTSIAARTLT
                     RHASGKIVADGEEAEAVGPLPVAALGAGDAITTGLRRAGLKTIGDVASRAPHELSARF
                     GAAFTTLLGHALGQGDAPISPRKPLPDYIVEKRFPEPVATDAVITLTLQNLARMLVTA
                     MDRQG"
     gene            complement(1090..1902)
                     /locus_tag="VG274_11690"
     CDS             complement(1090..1902)
                     /locus_tag="VG274_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07365.1"
                     /translation="MNGARTGTLASLRGSIARIEAPSDANMPNRVALGHTGADAMLRG
                     GLAAAALHEVFAGGHQSGAATGFVAGLAGRLSPRKPLVWVRQDFSDRESGALSMSGLA
                     ELGLDPRLLVTVRAADVDSGLRTAADALACDALGAVVLEVWGDVRQLDLVASRKLTLA
                     AQASGVTALVLRIAATPIPSTAETRWIVRAAHSPPGAAAMPAAPCSAWGAPRFDAELV
                     RNRHGPVGRWIMDWKCDECQFSEPSAHPQPLAATPPHRPHHAQARAQHRLAS"
BASE COUNT          374 a          741 c          740 g          331 t
ORIGIN      
        1 ccctgcctgt ccatcgccgt caccagcatc cgggcgaggt tttgcagcgt cagcgtgatc
       61 acggcgtcgg tggcaacagg ctcgggaaaa cgcttctcga cgatgtaatc gggcagcggc
      121 ttgcgcgggc tgatcggcgc atcgccctgc cccagtgcgt ggccgagcag cgtcgtgaag
      181 gccgcgccga accgcgccga tagctcgtgc ggcgcgcgcg acgcgacatc gccgatggtt
      241 ttcaacccgg cacggcggag ccccgtggtg atcgcgtcgc cggcgccgag cgctgcgacc
      301 ggcagcggac cgacggcttc cgcttcctca ccatcggcga caatcttgcc cgaggcatgc
      361 cgcgtcagcg tgcgtgccgc gatggaggtg ccggcgattg cggcgctgac cgcaaagccg
      421 cggcgggtca gcgcgcccgt cacgatctgc agcagcgcgc gctcgccgcc gaatagatgc
      481 gcgcagccgg taatgtcgag gaacaggccg tagggcggat ccagcgccac aagcggcgtg
      541 aagcgatcgc accagtcggc gatgtcgttc agcgtcttca agtcggcggc gggatcggcg
      601 tcgtagacgg tcagctccgg gcagatcgcg cgggcattgg cgagcggcag cccgatcgaa
      661 aggccggcgc gcaccgcggc ctcgtcgagc gcgtggagca ggatggcgtt gtgatccttt
      721 gcgacgacga cgctggggag ttcgttgttc gtgagggacg catcaataga cgggttccct
      781 ccccccttgt ggggtccgag gcgagcgaag ctcgctctcg aggttaggga gaggggtacc
      841 acggactccg gcgtttgcgg cttacccctc tctccaactc tcccccgcaa ggggggagag
      901 agcccagcca cctcccgagt atcaccccac cggaggtctt gcttcgcctc tcccgcttgc
      961 gggggaagcc ggatcgcatc gcgagatgcg atccgggtgg gggttctctc ctcagcgaga
     1021 ctcgtggaga gacccccacc ccaaccctcc cccgcaagcg ggagagggag ccctgacacc
     1081 tcccacgcgt cagcttgcga gacggtgctg tgcgcgagct tgcgcgtgat gcggtcgatg
     1141 gggaggcgtg gcagccagag gctgaggatg cgccgacggt tcactgaatt ggcactcatc
     1201 acatttccag tccatgatcc atcgcccgac cgggccatga cgattgcgca cgagctcggc
     1261 atcgaaccgc ggcgcgcccc aggcgctgca gggcgccgca ggcattgcgg ccgcgcccgg
     1321 cggcgaatgc gccgcgcgca cgatccagcg cgtctccgcg gtcgagggaa ttggcgtcgc
     1381 cgcgatccgc agcaccagcg cggtgacgcc ggaggcttgc gctgccaagg tcaatttgcg
     1441 gctggcgacg agatcgagct ggcgtacatc accccagacc tccagcacga ccgcgcccag
     1501 cgcgtcgcag gccagcgcgt cggcggcggt gcgcaagccg ctgtcgacat cggcggcgcg
     1561 caccgtgacc agcaaccgcg gatcgaggcc gagttcggcg agcccgctca tcgacagcgc
     1621 gccagattca cgatcggaaa aatcctgccg cacccagacc agcggcttgc gcggcgacag
     1681 acgtccggcg aggccagcaa cgaagccggt cgccgccccg ctttgatggc cgccggcgaa
     1741 cacctcatgc agtgcggccg ccgcaagccc gccgcgcagc atcgcgtccg cgcccgtgtg
     1801 gccgagcgcg acgcggttcg gcatgttcgc atccgatggc gcctcgatgc gcgcgatgct
     1861 gccgcgcaag gacgcaagcg tacccgtgcg tgcaccattc atagcgccgc tccttcccaa
     1921 cagaaatttg aagccgtcac tggttctgaa cagagaacca cgctggctca tttgttcatg
     1981 atatgttcta atataaagct aacaggaccg gcggagtcaa tcggattggt gggctctccg
     2041 attcatcagc ggaatcaagg ggattcagga gcagcccgga cgggacgcgg ctcccgcatc
     2101 ccttcccctc cgcatcatcg ggcgcgcgag ccctgaaatg gcggcggcgt gacggaaacg
     2161 gagcaaatcg catgcacacc atgatg
//
LOCUS       DASZMC010000574         3787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4377681,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000574 DASZMC010000000
VERSION     DASZMC010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4377681"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VG274_11695"
     CDS             complement(<1..243)
                     /locus_tag="VG274_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07366.1"
                     /translation="MKTDRPPDTVRAYLDALRNALKGSSPGLISDALADAEEHLQGEI
                     ASNPDKREAEILTSVIETYGTPQEIAEEYKSMETAIA"
     gene            complement(240..638)
                     /locus_tag="VG274_11700"
     CDS             complement(240..638)
                     /locus_tag="VG274_11700"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWE07367.1"
                     /translation="MADQESPYTRKFRKELMAGLSSLVLLAVLARAGREMYGYEIAKA
                     VKAEGEGGLIFKQGAIYPVLRSLHALGLLESRVEASAFGPPRRYYSITDEGRRALSDW
                     QGAWHDMREFVDDALAAQMSLRGPTLESRA"
     gene            837..2162
                     /locus_tag="VG274_11705"
     CDS             837..2162
                     /locus_tag="VG274_11705"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF008979.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWE07368.1"
                     /translation="MRLSSYFLPILKETPAEAQIVSHRLMLRAGLVRQEAAGIYAWLP
                     LGQRVLRRIEQIVREEQDRAGAIEILMPTLQPADLWRETGRYDAYGKEMLRIKDRHDR
                     EMLYGPTNEDMVTEIFRAYVRSYKSLPLTLYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDLDAAGARKSYNRQFVAYLRTFARMGLKSIPMRAETGPIGGDLSHEFIILADTGE
                     SQVYCNSDLIEMPVPGEDVDFDGDLEPIVSARSRLYAATEDVHDAAQFEAQVPAEKRL
                     SARGIEVGQIFYFGTKYSLPMRAFVAGPDGRDYPVESGSYGVGVSRLVGAIIEVNHDE
                     AGIIWPEPVAPFDFGLINLKTGDAATDGVCEDLYAKLNAAGREVLYDDTQERPGAKFA
                     TMDLIGIPIQIIVGPRGLEKGIVEFKTRRTGARVELSPDAAVNRFTQCR"
     gene            2502..3764
                     /locus_tag="VG274_11710"
     CDS             2502..3764
                     /locus_tag="VG274_11710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HWE07369.1"
                     /translation="MARGTRAFAPFEWMIALRYLRAKRQESFVSVISGFSLVGIAVGV
                     ATLIIVMAVMNGFRHELLSRILGLNGHLTVQAPTGTLSDFDAVAARVRAVPGVVRVAP
                     IVDGQVMGAANGLNAGVLVRGMRREDLRTLTTVSKSLSEGALSHFQSGDSVIVGRRLA
                     DRMRLAPGAQITLIAPRGNVTPFGVTPRVKSYTVAGTFQIGMSEYDQSFVFMPLDEAQ
                     LYFSMGSNVNGLEVMIRDPERVSAMVAPIARAAGPFSRIVTWQDMNSSLFAALQVERN
                     VMFLILTLIILVAALNIVSGLIMLVKDKSGDIAILRTMGAARGAVMRVFLIAGASIGV
                     FGTLLGFGIGVIFCLNIESIRQFLSSLTGTELFNPEIYFLSRMPAEMDPGEVISVVAM
                     SLSLSFLATLYPSWRAARLDPVEALRYE"
BASE COUNT          668 a         1176 c         1178 g          765 t
ORIGIN      
        1 cgcaatggcg gtttccatgg atttatactc ctccgcaatt tcctgtggcg ttccgtaggt
       61 ctcgatgacc gaggtcagga tctccgcttc tcgcttatcg ggattgctcg cgatctcgcc
      121 ctgaaggtgc tcttcggcat cggcgagcgc gtcgctgatc agacccggcg acgatccctt
      181 gagcgcgttg cgcaacgcgt cgagataggc acggaccgtg tcgggcggac gatccgtttt
      241 catgcacgac tctccagcgt cggcccccga agggacatct gtgctgcgag cgcatcatcg
      301 acgaattccc gcatgtcgtg ccacgcgccc tgccagtcgg agagtgcgcg ccgcccttca
      361 tcggtgatgc tgtagtagcg ccgcggcggg ccaaaggccg aagcctcgac ccggctctcc
      421 agcaggccca acgcatgaag agaccgcagg acaggataga tggcgccctg cttgaagatc
      481 aatccgcctt cgccttcggc cttcacggcc tttgcgatct cgtaaccata catctcgcgg
      541 ccggcccggg cgagcacggc caagaggacg agcgaggata gcccggccat gagttcctta
      601 cggaatttgc gcgtataggg ggattcttgg tctgccacgc gcctgtttcc gcctccttgc
      661 tattgcgaac ttcaatctag catgaacttc gtactatgca atagttcact atacggcagt
      721 tacgaatatt tttcatcggc cgttcaccct tcgcatccca cgaggcggga atttgccctt
      781 gtcgacagcc ttccctaaga aagacagcct tattgccaag gcaacggtcc ttcctcatgc
      841 gcctttccag ctatttcctg ccgattctca aggagacccc ggccgaggcg cagatcgtgt
      901 cacatcggct catgctgcgc gccgggctgg tccgccagga ggccgcgggc atctatgcct
      961 ggctgccgct cggccagcgg gtgctgcgca ggatcgagca gatcgtccgg gaggagcagg
     1021 accgcgctgg cgccatcgaa atcctgatgc cgacgctcca gccggccgac ctctggcgcg
     1081 agacgggccg ctacgacgcc tatggcaagg agatgctgcg cataaaggac cgccacgacc
     1141 gcgaaatgct gtatgggccc acgaacgaag acatggtcac cgaaatcttc cgcgcctatg
     1201 tgcgttcgta caagagcctg ccccttacgc tctatcacat ccagtggaag ttccgcgacg
     1261 aagtgcgacc gcgcttcggc gtgatgcgcg ggcgcgaatt cctcatgaag gatgcatatt
     1321 cgttcgatct cgacgcggcg ggtgcgcgca aatcctacaa caggcaattc gtcgcttatc
     1381 tgcgcacctt cgcgcgcatg ggtctcaaat cgattccgat gcgtgcggag accgggccga
     1441 tcggcggcga cctcagccac gagttcatca ttctcgccga caccggagag agccaggtct
     1501 attgcaacag cgatctgatc gagatgccgg tgcccggcga ggatgtcgat ttcgacggcg
     1561 atctcgagcc catcgtgagc gcgcggtcgc ggctctatgc ggcgacggag gacgttcacg
     1621 acgcagcgca attcgaggcg caggtgcccg cggagaagcg cctgtccgcg cgaggaatcg
     1681 aggtggggca gattttctat tttggcacga aatactctct tccgatgcgg gcgttcgtgg
     1741 ccggtccgga tgggcgcgat tatccggttg agagcgggtc atacggcgtg ggggtgtcgc
     1801 ggctcgtggg cgcgatcatc gaagtcaatc acgacgaggc aggaatcata tggccagagc
     1861 cggtcgcgcc attcgatttt ggattgatca atctcaagac cggcgacgcc gcgaccgacg
     1921 gagtttgcga ggacctctat gccaagctca acgccgcggg ccgcgaagtg ctctatgacg
     1981 acacccagga acgtcccggc gcgaagttcg cgaccatgga tcttatcggc atcccgatac
     2041 aaatcatcgt cggcccgcgg gggttggaaa agggaatcgt cgagttcaag acgcgaagga
     2101 ccggcgcacg cgtggaattg tcaccggacg ccgccgtgaa tcgtttcacg caatgtcgct
     2161 agtgaatcga ctgaaactct tgatacctaa gctgaccggc tcggcgcgtc gcgcgcagcc
     2221 ggcgaatcgc cactcgtcgc actgcgcgtg cggtgcagcg ggcagcgctc agccgcaagg
     2281 acgtcaacgg aaccagacac ttgcctttcc gcgtgtcatt cctgcgaaag cgggaaccca
     2341 aaagcagtgc ttgcggaatt tcttcacggt cctttgtgaa ctgtcgatct gggattcgcg
     2401 cgtcaaaact cagccacccg ctccatgtct tttcgctccc gctcacgctc ttgccgcgtc
     2461 gcgcccgcag cgcggcgagt gcagaaagct tgccgcctga catggcgcgg ggcacgcgcg
     2521 cgttcgcgcc gttcgagtgg atgatcgcgc tgcgctatct tcgcgccaag cggcaggaga
     2581 gtttcgtttc ggtcatctcc ggcttttcgc tggtcggtat cgcggtcggt gtcgccacgc
     2641 tcatcatcgt catggcggtg atgaacggct tccgccacga attgctgtcg cgaatcctcg
     2701 ggctgaacgg ccacctgact gtccaggcgc cgacgggcac cttgtcggat ttcgatgccg
     2761 tcgccgcacg ggtgcgcgcc gtgccggggg tcgtgcgcgt agcgccgatc gtagacggac
     2821 aggtgatggg agccgccaac ggtctcaatg ccggcgtgct ggtgcgtggc atgcggcggg
     2881 aagatttgcg tacgctcacg accgtgtcga agtcgctgtc ggaaggagcg ctctcgcatt
     2941 tccagagcgg tgattccgtg atcgtgggca ggcgccttgc cgaccggatg cggttggcgc
     3001 ccggcgcgca gatcacgctg atcgcaccgc gcggaaacgt cacgccgttc ggcgttactc
     3061 cgcgtgtgaa aagctacacg gtcgccggca cgttccagat cggcatgtcg gaatacgatc
     3121 agagcttcgt cttcatgccg ctcgacgaag cgcagctcta tttcagcatg ggctcgaatg
     3181 taaacgggct cgaagtgatg attcgcgatc ccgaacgggt cagcgcgatg gtggcgccga
     3241 tcgcccgcgc ggccggcccg ttctcgcgca tcgtcacctg gcaggacatg aactcgagcc
     3301 tgttcgcggc cttgcaggtc gaacgcaacg tcatgttcct gattctgacg ctgatcattc
     3361 tggtggccgc gctcaatatc gtttccggcc tcatcatgct ggtgaaggac aagagcgggg
     3421 acatcgccat tctgcgcacg atgggcgcgg ccaggggcgc ggtgatgcgc gtcttcctga
     3481 tcgccggcgc gtccatcggc gtgttcggca cattgctggg atttgggatc ggcgttatct
     3541 tttgcctcaa catcgaatcg atccggcagt ttctctcctc gctcaccggc accgagctgt
     3601 tcaatccgga aatctatttc ctgagccgca tgccggccga gatggacccg ggcgaggtga
     3661 tctcggtcgt cgccatgtcg ctctcgctct cgtttcttgc cacgctctat ccgtcatggc
     3721 gcgcggcgcg cctcgatccc gtcgaggcgc ttcgctatga gtgaggcagc ggcgcttcgc
     3781 gtcgaag
//
LOCUS       DASZMC010000575         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1635764,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000575 DASZMC010000000
VERSION     DASZMC010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635764"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2530
                     /locus_tag="VG274_11715"
     CDS             <1..2530
                     /locus_tag="VG274_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HWE07370.1"
                     /translation="DEHYPDRMLLAEANQWPEDIQEYFGAGDECHMVFHFPLMPRMYM
                     AIAQEDRFPITDIIRQTPDIPHGCQWAIFLRNHDELTLEMVTDRERDYLWDVYAADRR
                     ARLNLGIRRRLAPLLENDRRRIELMNGLLLTMPGTPIIYYGDEIGMGDNIHLGDRDGV
                     RTPMQWSSDRNGGFSRADPSTLVLPPIMDPLWGYQAVNVEAQWRNPHSLLNWLRRMLV
                     ERRRHQAFGRGTLRLLLPQNRKVLAYLREYQDDTILCVVNVSRSAQAVELDLSEFAGR
                     TPIELLGGTPFPRIGQLTYLLTLPPYGFYWFLLSESAEAPAWSTATTGPFVEHQTFVL
                     RGGLAQALERSIKEELETDILPLYIPARRWFQNKDAKVGEVGIAAIAPLPGAEQDAVF
                     ADVDVAVGGQKVRYALPMAIAWEDAPPSPFETPLAFARARRVRRVGLLTDGFASASFA
                     HAILGGLARHETIPFGDGEIAFLPLGDAAANIPLDVEIEWQGAEQTNSTLVVGRRAVI
                     KLFRRVTKGIHPEPEMTRVLTERGFAGTPQLMGEVVRRNASGGTAALAVVEAYVTNQG
                     NGADWTRDYLARIIDEQAVAPSESARDAFDAYWVFVQALGRRVGEMHAILAEPCDDPA
                     FAPEKVDLAAVNAAWAGARDQLKAALKSLLHTEDRERRRLVARLAARRDEILGRLATL
                     LANGLGSTRMRIHGDLHLGQVLVCGGDVVIIDFEGEPIKPLATRRAKLSPMRDVAGMV
                     RSFDYAAGIIERERKLAAGAPGHARASELLEEFRRLAESKFLDAYGEGRGRPLDGGER
                     QIIEGFTIEKAAYEIVYETANRPDWVDIPLRGLARCLQREPEPT"
     gene            2724..>4298
                     /gene="glgB"
                     /locus_tag="VG274_11720"
     CDS             2724..>4298
                     /gene="glgB"
                     /locus_tag="VG274_11720"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HWE07371.1"
                     /translation="MNRNTGSSPDAVAVALLAQGRLSDPFSLLGPHDTPHGTCVRAFL
                     PGALEVDAIARGNGEWLGRLAHTDSVGLFEGPLSRNAPYMLRIRWPLATEETEDPYSF
                     GPLLGDLDLHLFSEGSHWNLAQQFGAAVVAVDGVTGVRFSVWAPNAQRVSVVGDFNSW
                     DGRRHPMRLRHSAGVWEIFVPRLAAGERYKYEIVDPSGAVLPLKADPLARATERPPAT
                     ASVVVRPWSFRWTDSDWMATRAQRQSRSAPIAIYEVHAASWLRPKLDPLGTLDWLALA
                     EHLIAYAGDLGFTHIELLPIMEHPFGGSWGYQPLAQFAPSARFGEPQEFAHFVDACHR
                     AGLGVILDWVPGHFPTDPHGLARFDGTALYEHADPREGFHRDWNTCIYNLGRREVQGF
                     LIASALYWLEMFHVDGLRVDAVASLLYRDYSREPGEWIPNRYGGRENLEAVEFLKRLN
                     AIVAERAPGAVTIAEESTAWPGVSAPLSDGGLGFSFKWNMGWMHDTLAYVSENPVHRR
                     WHHDWMTFGLLYAFSER"
BASE COUNT          712 a         1394 c         1393 g          799 t
ORIGIN      
        1 cgatgaacac tatcccgacc gcatgctgct cgcggaagcc aaccaatggc cggaagacat
       61 ccaggaatat tttggcgccg gcgacgagtg tcacatggtg ttccatttcc cgctgatgcc
      121 gcgaatgtac atggcgatcg cgcaggaaga ccgctttccg attaccgaca tcatccgtca
      181 gacgcccgat attccccacg gatgccaatg ggcgatcttc ctgcgcaacc acgacgaact
      241 gacgctcgaa atggtgaccg accgcgagcg cgactatctg tgggacgtct acgcagcgga
      301 ccgccgcgcg cggctcaatc tcggcatccg caggaggctt gcgccgctcc tggagaacga
      361 ccgccgccgc atcgagctga tgaacggcct gctgctcacc atgccgggca cgccgatcat
      421 ctattacggc gacgagatcg gcatgggaga caacatccat ctgggcgacc gcgacggcgt
      481 gcgcacgccc atgcaatggt cgtcggaccg caatggcgga ttcagccgtg ccgatccgtc
      541 aacgctcgtc ctgccgccga tcatggatcc gctctggggg tatcaggcgg tgaatgtgga
      601 agcgcaatgg cgcaatccgc actcgctgct caactggctt cgccggatgc tggtcgagcg
      661 ccggcgtcac caggcgttcg ggcgcggcac tctgaggctt ctgcttccgc agaaccggaa
      721 ggtgcttgcc tatctgcgcg aatatcagga cgacacgatc ctctgcgtcg tcaatgtttc
      781 gcgctccgca caggcggtcg aactcgatct gagcgaattt gccggacgta cgcccatcga
      841 gctgctgggc ggaacgccgt ttcccaggat cggccaactc acctatctgc tcacactgcc
      901 gccctacggt ttttattggt tcctgctcag cgagagcgcg gaggcgcccg cgtggagcac
      961 ggcgacgacg gggccgttcg tcgagcatca gacattcgtg ctgcgcggcg gtcttgcgca
     1021 ggcgctcgag cgttcgatca aggaggagct cgagacggat atcctgccgc tctatattcc
     1081 cgcgcggcgc tggtttcaga acaaggatgc gaaggtcggc gaggtcggga tcgcagccat
     1141 cgcgcccctg cccggcgccg aacaggacgc ggtctttgcc gatgtggacg tggcggtcgg
     1201 gggccagaag gtgcgctacg cgctgccgat ggcgattgcc tgggaagacg cgcccccctc
     1261 tcccttcgag acgccccttg cctttgcccg cgcgcgccgc gtccggaggg ttggcttgct
     1321 caccgacgga ttcgcttccg cgagttttgc acacgcgatt ctgggcggcc tcgcgcgcca
     1381 cgaaacaatt cccttcggcg atggcgagat tgccttcctc cctcttggcg acgctgcggc
     1441 caacatcccg ctggatgtgg agatcgagtg gcagggcgcc gaacagacca actccactct
     1501 ggtcgtcggc cgccgcgcgg tcatcaagct gtttcggcgc gtcacgaaag gcattcatcc
     1561 cgaaccggag atgacgcgcg tgctgaccga acgcgggttc gcgggtactc ctcagctgat
     1621 gggcgaagtg gtgcgccgca atgcatccgg gggaacggct gcgctcgccg tcgtcgaagc
     1681 ctacgtcacc aaccagggaa acggcgcgga ctggacccgc gactatctcg cccggatcat
     1741 cgacgagcag gctgtcgctc cgagcgaaag cgcccgcgac gcgttcgacg cctattgggt
     1801 cttcgtgcag gcgctcggcc gccgggtggg agaaatgcat gcgatcctcg ccgagccctg
     1861 cgacgatccg gcgttcgcac ctgaaaaggt cgatctggct gccgtgaatg cagcctgggc
     1921 aggcgcgcgc gaccagctga aagcggcgtt gaaatctctc ctgcacacgg aagaccggga
     1981 acgccgccgg ctcgtcgccc ggctcgccgc ccggcgggac gagattctcg gacgcctcgc
     2041 aacgttgctg gccaacgggc ttggatcgac gcgcatgcgc atccacggcg accttcatct
     2101 ggggcaggtg ctggtctgcg gcggcgacgt ggtcatcatc gatttcgaag gcgaaccgat
     2161 caagccgctt gccacgcggc gcgccaagct cagccccatg cgcgatgtgg cggggatggt
     2221 ccgctccttc gactatgccg caggcatcat cgaacgcgag cgaaagctcg ccgcgggtgc
     2281 gcccggccat gcgcgggcgt ccgaactgct cgaggaattc cgcaggctgg ccgaaagcaa
     2341 attcctcgac gcttacggag aaggccgggg acgtcccctc gatggtggcg agcgccagat
     2401 catcgaaggg ttcacgatcg agaaggccgc ctacgagatc gtctacgaaa cggcgaaccg
     2461 gccggactgg gtcgatatcc cgttgcgcgg tcttgcccgc tgcctgcagc gcgagcccga
     2521 acccacctga tgccaattgt tgatcccgag atagccatgc gccagcagat tttaacgcag
     2581 agtacgcaga ggtttcgcgg aggacgcaga gggatttctt gctcgctccg cgcgctgtca
     2641 tctttccgcg tcccttgcgc tgcctccgcg ccctctgcgt taaatctgca cttggtttca
     2701 tacgtgccta aattgaggcc ctggtgaaca ggaacacggg gagcagcccc gacgctgttg
     2761 cggtcgcgtt gctcgcgcag ggcaggcttt cggatccgtt ttccttgctc ggtccgcacg
     2821 acacgccgca cgggacttgc gtgcgagcgt ttcttcccgg cgctttggaa gtggacgcga
     2881 tcgcccgcgg caacggcgaa tggctcggac gcctggcgca taccgattcc gtgggattgt
     2941 tcgaggggcc gttgtcgcgc aacgcgccgt atatgctgag gattcgctgg ccgctggcga
     3001 cggaggagac cgaggatccc tattctttcg ggcccttgct cggcgacctc gatctgcatc
     3061 tgttttcgga gggctcgcac tggaatctcg cgcagcaatt cggcgctgcg gtggtggcgg
     3121 tcgacggcgt gaccggagtg cgcttctcgg tttgggcgcc caatgcgcaa cgcgtttcgg
     3181 ttgtcggcga tttcaattcc tgggacgggc ggcggcatcc gatgcggctg cgccactccg
     3241 ccggcgtttg ggagatcttt gtgccgcgcc tcgccgcggg cgaacgctac aaatacgaga
     3301 tcgtggatcc ctccggcgcg gtgctgccgc tcaaggccga tccgctggcg cgcgcgaccg
     3361 agcggccgcc ggcaacggcg tcggtcgtgg tacggccatg gagtttccgc tggacggatt
     3421 ccgactggat ggcgacgcgc gcgcaacgcc agtcccgttc cgctccgatc gccatctatg
     3481 aggtgcatgc cgcttcctgg ctgcggccga agcttgatcc gctgggcacg ctcgactggc
     3541 tcgcgctcgc cgagcatctg attgcgtacg cgggcgatct cggcttcacc catatcgaac
     3601 tcctgcccat catggagcat cccttcggcg gctcatgggg ctatcagcct ctcgcacagt
     3661 ttgcgcccag cgcccgcttc ggcgagccgc aggaattcgc gcatttcgtc gacgcgtgcc
     3721 accgcgcggg actgggcgtg atcctggact gggtgcccgg tcattttccc accgacccgc
     3781 acggtctcgc gcgcttcgac ggcacggcgc tctacgaaca cgcggacccg cgcgagggtt
     3841 tccaccgcga ctggaacacc tgcatctaca atctgggcag gcgcgaggtg cagggcttcc
     3901 tcatcgcaag cgcgctctat tggcttgaaa tgtttcatgt cgacggattg cgcgtcgacg
     3961 cggtggcgtc gctgctctac cgcgactatt cgcgtgagcc tggcgaatgg attccgaacc
     4021 gctacggagg ccgcgagaat ctcgaagcgg tggaattcct gaagcggttg aacgccatcg
     4081 tggccgagcg cgcgccgggc gccgtgacga tcgcggagga gtcgacagca tggccgggag
     4141 tctccgcgcc attgagcgac ggtggcctcg gtttttcctt caaatggaac atgggctgga
     4201 tgcacgacac gctggcctat gtttccgaaa accccgtgca ccgccgctgg catcacgact
     4261 ggatgacgtt cggtctgctc tatgcgttct cggagcga
//
LOCUS       DASZMC010000576         5034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2985417,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000576 DASZMC010000000
VERSION     DASZMC010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2985417"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..901)
                     /locus_tag="VG274_11725"
     CDS             complement(161..901)
                     /locus_tag="VG274_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWE07372.1"
                     /translation="MRILLALLAPLVALAQPQNPAPTVIPPPLRQVAGLINDYANTRR
                     YAAENATVKPPAPGENRVVFMGDSITDNLHNPRMGGFFPGKPYLNRGISGQTTSQMLL
                     RFYPDVIALQPQAVVIFAGTNDIGGNLGPVSIESIEDNLAAMSDIARAHNIKVIMASV
                     TPVCDIPGRNPMTVGRPPESILTLNRWIKDLTAKQGYVFLDYFTATVDDKGFFRAELT
                     EDGLHPTAKGYEIMNPLAEKAIAQALGR"
     gene            complement(911..1270)
                     /locus_tag="VG274_11730"
     CDS             complement(911..1270)
                     /locus_tag="VG274_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07373.1"
                     /translation="MKLVLAGLLLLTCAFAAQTRWSDSDRAQAREERVAQRSDARRWR
                     LLNDQDERRLRVEAARVRAEAARDRVQMRRAFANERVRFGRSLREEQSRLRDEGRELR
                     ERTRELQREMRDRFRWN"
     gene            complement(1337..2197)
                     /gene="aroE"
                     /locus_tag="VG274_11735"
     CDS             complement(1337..2197)
                     /gene="aroE"
                     /locus_tag="VG274_11735"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWE07374.1"
                     /translation="MPANFKAELLACFGQPVAENPTGAMQEAAFRALGLDWRYLTVEV
                     PPDRLREAVSGARAFGMQGFNLTIPHKVAVIGLLDEVAPDAALIGAVNTVRRSGERLI
                     GENTDGKGFLRGVREKGSLDPKGRRVVVLGAGGAARAIVAELALAGAADILVINRSAP
                     RGEALVRDLADRTKAPIRFAPWQAAHIVAPETDLLVNATSIGLYPDVKAMPPVDLGGA
                     NPQLLVCDVVFNPPITPFLQAAIDRGLAVVDGLAMLVFQGVIGFELWTGRKPPEGVMT
                     AALRQALGLA"
     gene            2253..3656
                     /locus_tag="VG274_11740"
     CDS             2253..3656
                     /locus_tag="VG274_11740"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWE07375.1"
                     /translation="MRFCLFLLTLALLAQAQEFDTVIANGRVMDPASGLDAVRYVGIR
                     GGKIAAVSSTALRGRATIDAKGLVVAPGFIDLHSHGQTPENYRYKAMDGVTTALEMEV
                     GASPIPAWYAEREGKALINFGASSGYLPARMAVMHDTGTLLPRDNAVKRDATAEEQKQ
                     MLALLRRGVDDGALGVGLGIAYIPLASRPEILDVFRVAAEKKTAIYVHIRNGGPQEPG
                     VIDAMQEALADAAVTGASLHVVHITSMGLRQTPLCLEMIEGARKRGLDVTTEAYSYTA
                     GMTDIASAIFDDGWQARQGGITFHDLQWAATGERLTPESFARFRKQGGMVAVHSIPED
                     IVKLAIANPMVMIASDGILENGKGHPRAAGCFTRVLGRYVREQHALTLMEALRKMSIA
                     PANRLGVPTKGRIAVGADADVAIFDPAKVIDRATFENPAQYSEGMVYVLVNGVPVVQK
                     GALVDGIFPGRPLRRGH"
     gene            complement(3653..>5034)
                     /locus_tag="VG274_11745"
     CDS             complement(3653..>5034)
                     /locus_tag="VG274_11745"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWE07376.1"
                     /translation="LRGIRNGYLSTRYGAKRADAAGTTLTLEAGAQFKDLQLKLIPFA
                     VVAGTVLDPDGEGLAEARVALIAVRYRNGHRQAVATGQYATTDEAGRFRIPSVPPGSY
                     YLHAGPPEQVGEWEDHSRKGSPEEILVPAFYPATTDIGSARKFDVNAGDRLEGADITL
                     IRSKVYRVRVRLESPEGLRSGVGLDPRPNLSDGISPNLPSNCRNGVCEFRHVPAGSYT
                     ASAQISRPQMTITEMFSSHDRIEMPVDVTNGDVDDVHILITQPSEVTGHITIEESGEK
                     PKLDFGVRFITPSGDDLSSRVGSDGSFGARLSRGLYEVEPYSPSGLIVKSMRSGDVDV
                     LREGLNINKPEKVPLEIVLSRDGAVLDGVVRDKDDHAVSAATVVLVPENALRARIGLY
                     QVVSSDQLGHYHFAAVMPGSYKLFAWSYIEPDSWFDVDVLKDFESFGSPITLDAKAHA
                     NADLRVIVH"
BASE COUNT          957 a         1394 c         1724 g          959 t
ORIGIN      
        1 atatcgcaga ccggcgtaac agacgccatg atgactttga tgttgtgagc gcgggcgatg
       61 tcgctcatgg ccgcgagatt gtcttcgata gattcaatgg agaccggccc aaggttcccg
      121 ccaatatcgt tcgtgcccgc gaaaatcacg gcgcggatgg ctagcggccg agggcctgtg
      181 cgatggcctt ttcggcgagc gggttcatga tctcgtagcc tttggcggtg gggtgcagcc
      241 catcttccgt gagctccgcg cggaagaacc ctttgtcgtc gacggtagcg gtgaagtaat
      301 cgaggaagac gtatccctgt ttggcggtga ggtctttgat ccagcgattg agcgtcagga
      361 tcgactcggg cggacggccg accgtcatcg ggtttctacc gggaatatcg cagaccggcg
      421 taacagacgc catgatgact ttgatgttgt gagcgcgcgc gatgtcgctc atggccgcga
      481 ggttgtcttc gatagattcg atcgagacgg gcccgaggtt cccgccgata tcgttagtac
      541 cggcgaaaat cacgacggct tggggctgga gcgcgatcac atccggatag aagcgcaaca
      601 gcatttggga ggtggtctgc ccgctgatgc cgcgattgag gtacggcttg cccgggaaaa
      661 atccacccat gcgcggattg tgcagattgt ccgtgatgga gtcccccatg aagacgacgc
      721 ggttttcgcc gggggccggc ggcttgacag ttgcgttctc ggccgcataa cggcgcgtgt
      781 tggcgtaatc gttaataagc cccgcaacct gccgcaaagg cggcggaatt acagtgggag
      841 ccgggttctg gggttgggcc agcgcaacta ggggcgcgag gagagcgagc agaattcgca
      901 tcccgctatt ctaattccag cggaagcggt cgcgcatttc gcgctggagt tcgcgggtgc
      961 gttcgcgaag ctctcggcct tcgtcacgaa gccggctctg ttcttcacga agcgagcggc
     1021 cgaaccgcac acgttcattc gcgaaggcgc ggcgcatttg gacgcggtcg cgggcagctt
     1081 cggcgcggac tctcgcggcc tccacacgga ggcggcgttc gtcctggtca ttaagcaggc
     1141 gccagcggcg tgcgtcggag cgctgggcga cgcgttcctc gcgggcttgg gcgcggtccg
     1201 aatctgacca acgggtttgc gctgcgaagg cgcaggtgag caggagcaat ccggcgagta
     1261 cgagtttcat gcgtcaacct tccaatcctg atactccggg ggactgattt ggttacgaaa
     1321 attgtggcgc ggaagttcag gccaatccga gcgcctggcg gagagcggca gtcatgacgc
     1381 cttcgggcgg cttgcggccg gtccagagct cgaagccgat cacgccttga aagacaagca
     1441 tggcaagacc atcgactacc gcgagaccgc ggtcgattgc ggcttgcaga aacggcgtga
     1501 tcggcgggtt gaatacgacg tcgcagacga gcagctgcgg atttgcgccc ccgagatcga
     1561 ccggcggcat ggctttcacg tccggataaa gcccgatgga tgtagcgttg accagcaggt
     1621 cggtttcggg tgcgacgatg tgtgcggctt gccagggggc gaaacggatg ggcgcttttg
     1681 tgcgatcggc gagatcgcgg accagagctt cgccgcgcgg ggcagagcgg ttgatgacca
     1741 ggatatcggc tgcgccagcg agggccagtt cggcaacgat ggctcgcgcg gcgccgcctg
     1801 cgccaagaac gacgacgcgc ctgcctttcg ggtcgaggct acccttttca cgaacgccgc
     1861 gcaggaaccc cttgccgtcg gtgttttcgc cgatgagccg ctcacccgaa cggcgcacgg
     1921 tgtttacggc gccgatgaga gcggcatcgg gcgcgacttc atcgagcaga ccgatcaccg
     1981 ctaccttgtg agggatggtg aggttgaagc cttgcatgcc gaaggcgcgg gctccggaga
     2041 cggcctcgcg aagccgatcc ggcggaactt ccacggtaag atagcgccag tcgagcccga
     2101 gggcgcggaa ggccgcttcc tgcatggcgc cagtgggatt ttcggccacg ggttgtccga
     2161 agcaggcaag aagttcggcc ttgaaattcg ccggcatggg aatcaaatta acatggcatt
     2221 tgcgcgcgga ctagtggcgc gtggagatac acatgcgatt ttgcctcttc ctcttgacgc
     2281 tggcgctctt ggcacaggcc caggaattcg acactgtgat tgcgaacggc cgggtcatgg
     2341 atccggcgag cggcctggac gcggtgcgct atgtagggat tcgcggcggc aagatcgcgg
     2401 cggtttcgag tactgcgttg cgggggcgag cgacgatcga tgcgaaaggg ctggtggttg
     2461 cgccgggctt catcgatctg cactcgcatg gacagactcc cgagaactat cgctacaagg
     2521 cgatggacgg ggtgacgacg gcgctcgaga tggaggtcgg cgcgtcgccg attcccgcgt
     2581 ggtacgcgga gcgcgaaggc aaagcgctga tcaatttcgg cgcgtcgtcg ggctatttgc
     2641 cggcgcgcat ggcggtgatg catgacaccg gcacgctgct gccgcgcgat aacgccgtga
     2701 agcgcgatgc gaccgccgaa gagcagaaac agatgctggc tttgttgcgg cgcggagtgg
     2761 acgatggcgc gctcggcgtg gggctgggca tcgcgtatat cccgcttgcg tcgcggccag
     2821 agattctgga tgtgtttcga gtggcggcgg agaaaaagac cgcgatttac gtgcacatac
     2881 gcaacggtgg cccgcaagag ccgggtgtga tcgacgcgat gcaagaggcc ttggcggacg
     2941 cggcagtaac aggcgcgtcc ctgcacgtgg ttcacatcac cagcatgggc ttgcggcaga
     3001 cgccgctatg tctcgaaatg atcgaggggg cccggaagcg tggcttggat gtgacgacgg
     3061 aggcgtattc gtacacggcg ggcatgacag acattgcgtc ggcgattttc gacgatggat
     3121 ggcaggcgcg gcagggcgga atcacgtttc acgacctgca gtgggccgcg acgggcgagc
     3181 gattgacgcc ggaaagcttc gcgcgctttc gaaaacaggg cggaatggtg gccgtgcact
     3241 cgattccgga ggacatcgtg aagctggcga tcgcgaatcc gatggtgatg attgcctcgg
     3301 atggcatttt ggagaacggc aagggtcacc cgcgcgccgc cggatgcttt acgcgcgttt
     3361 tggggcgcta tgtacgcgaa caacacgcgc tgacgctgat ggaggcgctg cgcaagatgt
     3421 caattgcgcc cgcgaaccgg ttgggcgtcc caacgaaggg ccgaattgcg gtaggcgctg
     3481 acgccgatgt tgcgattttc gatccggcga aggtgattga ccgcgccacg ttcgaaaatc
     3541 cggcgcagta ttccgaggga atggtctatg tgctggttaa cggcgtgccc gtggtgcaga
     3601 aaggcgcgct ggtggacgga atcttccccg ggcgaccgct gcgccgcggc cactaatgaa
     3661 cgatgacgcg cagatcggcg ttggcgtgag ctttggcatc gagtgtgatg ggcgaaccga
     3721 agctttcgaa gtccttgagc acgtcaacat cgaaccatga atccggttcg atatagctcc
     3781 acgcaaacag cttgtaagac ccgggcatga cggctgcaaa gtggtagtgg ccgagttgat
     3841 cgctgctcac cacttgataa agaccaatgc gcgcccgcaa cgcattttcc gggaccagca
     3901 cgacagtagc ggcggagacg gcgtgatcgt ccttgtcccg aacgacgcca tcgagcaccg
     3961 cgccgtcgcg ggaaagcaca atttcgagtg gaaccttctc cggcttgtta atgttgagcc
     4021 cttcgcgcag cacgtctacg tctccggaac gcatgctttt cacaatcaaa ccggacggcg
     4081 agtagggctc cacttcgtaa agtccacgcg agaggcgggc gccgaaagaa ccgtcggatc
     4141 cgacgcgtga cgagaggtca tcgcccgaag gagtaatgaa cctgacgccg aagtcgagct
     4201 tcggtttttc gccgctctct tctatcgtaa tgtggccggt cacctcggat ggttgggtaa
     4261 tgaggatatg aacgtcatca acgtcgccat tcgtgacatc gactggcatt tcgatcctat
     4321 cgtggctcga gaacatttcg gtgatggtca tttgcggacg gctgatctga gcgctggctg
     4381 tgtaggagcc ggcgggaaca tgccggaact cgcagacgcc attccggcaa ttggatggca
     4441 gattgggcga gattccatcg ctcagattcg gacgcgggtc caagccgacg ccggagcgga
     4501 ggccttcggg actttcgaga cgaacgcgaa cgcggtagac cttgctgcgt ataagcgtga
     4561 tgtccgcgcc ctcgaggcgg tcgccggcgt tcacgtcgaa cttgcgggcg gagccgatat
     4621 cagtggtggc ggggtaaaag gcgggaacaa gtatttcctc tgggctgcct ttgcgcgaat
     4681 gatcctccca ctcgccaact tgttcaggcg gcccggcgtg cagatagtac gatccgggcg
     4741 gaacggatgg gatacggaag cggccggctt cgtcggtagt ggcatattgc cccgttgcga
     4801 cagcctgccg gtggccattg cggtagcgca cggcgatcaa ggcaacacga gcctctgcga
     4861 gaccttcgcc atcaggatcg aggacagtcc ccgcgacaac tgcgaagggg atcaacttca
     4921 gttgcaggtc tttgaactgc gcgccggctt cgagcgtaag cgtggtcccc gccgcgtctg
     4981 ctcgtttcgc gccatagcgc gtcgacaagt agccgttgcg aatgccgcgc agtc
//
LOCUS       DASZMC010000577         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2713870,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000577 DASZMC010000000
VERSION     DASZMC010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713870"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2180
                     /locus_tag="VG274_11750"
     CDS             <1..2180
                     /locus_tag="VG274_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE07377.1"
                     /translation="NALVHNGVVFPPTLLAMLITLFVGLIAACTMVASFAGQQNELAQ
                     NLKEEIARGDRARKEAEAANAAKSQFLATMSHEIRTPMNGILGIANLLSLTSLNDRQE
                     RFVENLNRSGQALLGVINDILDFSKIEAGRFELFEVEFDLRDMIGDVVDLFSERCASA
                     GLEFIYEIAEDVPSHLRGDPVRLRQVLVNLISNAIKFTERGDIFLKVAAESRAADRVV
                     LACSVEDTGIGIAAADQDKVFQSFQQLDGSMTRVRGGSGLGLAITKQLVELMGGHIGF
                     ESESGRGSRFRFTVQMGCPASAKRDAPAKHTFPDTFRVLVADSNAASARVLSRYLGSW
                     NINADVVTTTAEAETRWREAANEGADFTVATIDLKGFGRAGVDLARMIKAGHRPSKLI
                     LLVGMEATVADERIGELGAVATLAKPARPSELYNCLAHLAAASEERDLRAFSTKRKQR
                     EKRLSFDGRILVAEDNLVNREVVTGILESMGMEVVTAPNGRAALDLFMQDKFDVVLMD
                     CEMPVIDGLEAARRIRAFETRKSPADETRRTPIIALTAHALIEVRERCLRAGMDDFLT
                     KPFNEMQMGEALRRWLTPVVRAAVTTAPELETNLLPAPAPAIDTSVFAGIRAFQGSKG
                     AARLRQIFSRFLVEAPSLVKTIRSMHAEGNRDAVWRTAHSLKSSSAALGALQLSSRCA
                     TIELMARENGLEQVTPHLLALDADLAAATGGLQTFMESADVAA"
     gene            2167..>2907
                     /locus_tag="VG274_11755"
     CDS             2167..>2907
                     /locus_tag="VG274_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWE07378.1"
                     /translation="MSPPEVPATTSILIVDDDEVVREIMSATLEDAGFNVATAADGET
                     ALKLCIENPPDLLIADVLMPGMDGFQLCQELRQRPETGYIPILVATGLDDVPSIVRAF
                     DVGATDFINKPIKWIILNYRVQYMLRTSRVAQELRENQVRLLSAKEAAESANRAKNEF
                     LANMSHELRTPLNAVIGFSSVMHQEEFGPMPDKYREYSKVIEESGTHLLTIINSILDL
                     ARADASRLTLNEQEVEIADVVAFCEKIIL"
BASE COUNT          604 a          873 c          894 g          536 t
ORIGIN      
        1 acaacgcgct ggtccacaac ggagtggtct ttcctcccac gctgctcgcc atgctgatca
       61 cgctgttcgt agggctgatc gcggcgtgca cgatggtggc aagtttcgca ggtcagcaga
      121 acgaacttgc ccagaacctg aaggaggaaa ttgcgcgcgg cgacagggcg cgcaaggaag
      181 cggaggcggc caacgcggcg aaatcgcaat tcctcgcgac aatgagtcac gaaatccgca
      241 cgccgatgaa cggaattctc ggcatcgcga acctgctctc tttgacatcg ctgaacgatc
      301 gccaggaacg gtttgtcgag aatttgaacc gttcgggaca ggcgctgctg ggcgtcatca
      361 acgacattct cgatttctcc aagatcgagg ccgggcgctt cgaactgttc gaagtcgaat
      421 tcgatttgcg cgacatgatc ggcgacgtcg tcgatctttt ttccgagcgc tgcgcgagtg
      481 ccgggctcga attcatctat gagatcgccg aagacgtgcc atcgcatctg cgcggcgatc
      541 ccgttcgcct gcgccaggtt ctggtcaatc tcatcagcaa cgccatcaag ttcaccgaac
      601 gcggcgatat ctttctgaag gtcgcagccg agtcccgcgc cgccgatcgg gtggtcctcg
      661 cctgctcggt cgaggatacg ggcatcggca tcgccgccgc cgaccaggac aaggtctttc
      721 aatccttcca gcagctggac ggatcgatga ctcgcgtgcg gggtggatcc ggactcggtc
      781 tcgccattac aaagcagttg gtagaactga tgggcggcca tatcggtttc gagagcgaaa
      841 gcggccgcgg atcgcggttc cgctttacgg tgcagatggg ctgcccggct tccgccaaac
      901 gtgacgcgcc ggcgaagcat accttccccg ataccttccg tgtactggtc gcggacagca
      961 acgcggccag cgcgcgagtc ctgtcgcgat atcttgggag ctggaacatc aatgcggatg
     1021 tcgtcaccac cacggcggag gcggaaacga ggtggcggga ggctgcgaac gagggcgccg
     1081 atttcacagt cgcgaccatc gatctcaagg gcttcggacg cgccggcgtc gatctcgcga
     1141 gaatgataaa ggccggccac cggccgtcga agctgatcct gctcgtcggc atggaagcca
     1201 cggtcgcgga tgaacgtatt ggcgagcttg gcgccgtcgc cacactcgca aaaccggctc
     1261 gtccttccga attgtacaat tgcctcgcgc atctggccgc tgcaagcgaa gagcgcgacc
     1321 ttcgagcttt ctctacgaaa cgcaaacagc gcgagaaacg cctgtcgttc gacggccgca
     1381 tattggtggc ggaagacaat ctcgtgaatc gggaagtggt gaccggcatt ctcgaatcca
     1441 tgggaatgga ggtcgtcacc gcgccgaacg gtcgggcggc gctcgacctg ttcatgcagg
     1501 acaaattcga cgtggtgctg atggactgcg agatgccggt catcgacggc ctggaagccg
     1561 ccaggcgcat ccgcgcattt gaaacgcgca aaagtcccgc ggatgaaaca aggcggacgc
     1621 ccatcatagc gttgaccgcg cacgccctga tcgaggttcg cgaaagatgc ctgagggccg
     1681 gcatggatga tttcctcacc aagccgttca acgagatgca gatgggcgag gcattgcgcc
     1741 gctggttgac gccggtcgtc cgcgcggcgg tgacgaccgc accagagctc gaaacaaatt
     1801 tgcttcccgc gcctgcaccc gcgatcgata cgagtgtctt cgccggaatt cgcgccttcc
     1861 agggcagcaa gggagcggcg cgcttgagac agatattctc ccgtttcctt gtggaagcgc
     1921 cctcacttgt gaaaaccatt cgctcgatgc atgcggaagg aaaccgggac gccgtatggc
     1981 gcacggcgca cagcctcaaa tccagctccg cggcgctggg agccctgcaa ctctcgtcgc
     2041 gctgcgcaac gatcgagctg atggcgcggg agaatggact ggagcaggtg acgccgcacc
     2101 ttctggcgct cgacgccgat cttgccgccg cgaccggggg gttgcagaca ttcatggaga
     2161 gcgccgatgt cgccgcctga agtcccagca acgacctcca tcctcattgt cgatgacgat
     2221 gaggttgtgc gcgaaatcat gtcggccacg ctcgaagacg cgggattcaa tgttgcaacg
     2281 gccgccgatg gcgaaaccgc gctcaaactc tgcatcgaga accctccgga cctgctgatt
     2341 gcggatgtcc tcatgccggg catggatggc tttcagctgt gccaggaact ccggcagcgc
     2401 ccggagaccg gatatattcc cattctcgtc gcgacgggtc tcgatgatgt cccctcgatc
     2461 gtcagggcgt ttgacgtggg ggcgacggac ttcatcaaca agccgatcaa atggatcatc
     2521 ctgaactacc gcgtgcaata tatgctgcgc acgagccgcg tggcgcagga gctgcgcgaa
     2581 aaccaggtgc ggctcctgtc ggcgaaggaa gcggcggaaa gcgccaaccg cgccaagaac
     2641 gagtttctcg ccaatatgag ccacgagctg cgcacgccgc tcaacgccgt catcggtttc
     2701 tccagtgtca tgcaccagga ggagtttgga ccgatgcccg acaaataccg cgaatattcg
     2761 aaagtgatcg aggaaagcgg aactcacctt ctcacgatca tcaacagcat cctcgacctc
     2821 gccagggccg acgccagccg gttgacgctg aacgagcagg aggttgaaat tgcggatgtc
     2881 gttgctttct gcgaaaaaat aatcctg
//
LOCUS       DASZMC010000578         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5184564,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000578 DASZMC010000000
VERSION     DASZMC010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5184564"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="VG274_11760"
     CDS             complement(<1..2033)
                     /locus_tag="VG274_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HWE07379.1"
                     /translation="METLLHESEIAWARPREPEYRSGEARELENELRRSLEGEVRFDN
                     GSRALYATDASNYRQVPIGVVVPKTVEDVLAAMELCRRLDAPVLARGGGTSLAGQCCN
                     VAVVFDFSKYLNKLIDLDPKAKTARVQPGIVLDWLRGEAERHQLTFGPDPSTHNHNTL
                     AGMIGNNSCGTHSVMAGKTQENVIELEVLTYDGLRMRVGETRRDEQDLIVSAGGRRGE
                     IYAGLKDIAARHGDEIRRRYPDIPRRVSGYNLPDLLPENGFHVARALVGTESTCALTL
                     EATVKLIWSPPGRTLVVLGYPDIYKAGDHVPEIMEFRPIACEAIDALLVENMKIKGLH
                     PRDLKLLPDGRGWLLVEFGGEDRKDSDNQAGRLMDSLKKHDDAPAMKLFDDPGEEKLV
                     WDIRESGLGATAWVPGEPVTWEGWEDSAVAPEKLGGYLRELCELYEKHGYEGALYGHF
                     GQGCVHTRITFDLLTAAGIANYRSFMDDATSLVVKYGGSLSGEHGDGQSKAEFLPKMY
                     GAEICEAFREFKSLWDPRGKMNPGKIVDPYPITENLRLGRHFAPKEPETYFHFVDDKN
                     SFTHAAMRCVGIGECRRQEKGTMCPSYRTTMEEKHSTRGRAHLLFEMMHGGVIGDGWK
                     SEEVFDALDLCLSCKGCKSDCPVNVDMATYKAEFLAHYYEGRLRPRHAWAMGW"
     gene            complement(2014..>2417)
                     /locus_tag="VG274_11765"
     CDS             complement(2014..>2417)
                     /locus_tag="VG274_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWE07380.1"
                     /translation="PAGMRIAAGEYGYDSFYFRRMLEAGAVDVLQADATRCAGVTGFN
                     AAGHLAAAFSIPFSAHTAPSLHAHAACALPGAINVEYFFDHYRIEQMFFDGALVPRKG
                     CLQPDASRPGFGLECKAADMEKFKVYGNAAA"
BASE COUNT          443 a          766 c          718 g          490 t
ORIGIN      
        1 atccagccca tcgcccacgc atggcgcggc cgcagccgtc cctcgtaata atgcgccagg
       61 aactcggcct tgtaagtcgc catgtccaca ttgacagggc aatcgctttt gcatcctttg
      121 caggaaaggc aaaggtcgag cgcgtcgaaa acctcctcgc tcttccagcc atcgccgata
      181 acgccgccgt gcatcatctc gaacagcaga tgcgcgcggc cgcgcgtgga gtgtttttcc
      241 tccatggtgg tccgatagct cggacacatc gtgcccttct cctgccgtct gcactcaccg
      301 atgccgacgc agcgcatcgc cgcatgtgta aagctgttct tgtcgtcgac gaaatggaag
      361 taagtctccg gctctttggg cgcaaagtga cggccgagcc gcagattttc cgtgattgga
      421 taaggatcga cgattttgcc cggattcatt ttgccgcgcg gatcccagag agacttgaat
      481 tcgcgaaacg cttcgcagat ttccgcgccg tacatctttg gcaggaactc ggccttcgat
      541 tgaccgtcgc cgtgctctcc ggagagcgat ccgccgtatt tgacgacgag cgatgtggcg
      601 tcatccatga acgagcggta gttcgcgata cccgccgcgg tcagcaggtc gaacgtgatt
      661 cgcgtatgca cgcaaccctg gccgaaatgg ccataaaggg cgccttcata accgtgcttt
      721 tcgtagagtt cgcacagctc gcgcaggtat cctccgagtt tttccggagc gaccgccgaa
      781 tcctcccatc cctcccacgt gacgggttcg cccggtaccc acgcggtcgc gccgaggccc
      841 gactcgcgaa tgtcccagac cagcttttcc tcgccgggat cgtcgaacag cttcatggcg
      901 ggtgcgtcat catgcttttt caggctgtcc atcagacgcc cggcctgatt atcggaatcc
      961 tttctgtctt cgcctccgaa ttccacgagc agccagccgc gtccgtcggg cagcagcttc
     1021 agatcgcggg gatgcagacc cttgatcttc atgttttcga caagcagggc gtcgatagct
     1081 tcgcaggcaa tcggccggaa ttccataatc tcggggacat gatcgccggc cttgtagatg
     1141 tccggatagc ccagcaccac gagcgttcgg cccgggggac tccagataag tttcacggtt
     1201 gcttcgagcg taagcgcgca cgtcgattcc gtgcccacga gagcgcgcgc cacatggaaa
     1261 ccgttttcgg gaaggagatc cggcaggttg taacctgaga cgcgccgcgg aatgtcggga
     1321 tagcggcgcc gaatctcatc gccatggcgg gcggcgatat ctttcaaccc ggcatagatc
     1381 tcgccgcgcc ggccgcccgc ggagacgatc agatcctgct cgtcgcgccg tgtttcgccc
     1441 acgcgcatcc gcaggccgtc gtaagtcaga acttcgagct ctatgacgtt ttcttgcgtc
     1501 ttgcccgcca tcaccgagtg cgtgccacag gaattgttgc cgatcatgcc tgcgagcgtg
     1561 ttgtggttgt gcgtcgacgg gtcgggcccg aacgtcagtt ggtgacgttc cgcttcgccg
     1621 cgcagccagt cgagcacaat gccaggctgc acccgcgctg tctttgcctt cgggtcgagg
     1681 tcaatgagct tgttcagata tttgctgaaa tcgaagacga cggcgacgtt gcagcactgt
     1741 cctgcaaggc tagtgccgcc gccgcgcgcc agcacgggcg cgtcaaggcg gcggcacaac
     1801 tccatcgccg ccagaacatc ctcgactgtt ttgggcacga ccacgccgat gggtacttga
     1861 cggtagttgg aggcatcggt ggcatagagg gcccggctgc cgttgtcgaa ccgcacttcg
     1921 ccttcgaggg accggcgaag ctcgttttca agttcgcgcg cctccccgct ccgatattcc
     1981 ggctcacggg ggcgcgccca cgcgatttcc gattcatgca gcagcgtttc catagacttt
     2041 gaatttctcc atgtcggcgg ccttgcattc gagtccgaag cctgggcgcg aggcgtccgg
     2101 ctgcagacac cctttgcgag gcacgagagc gccatcgaag aacatctgct caatccgata
     2161 gtggtcgaag aagtactcga cgttgatcgc gcccggcaga gcacacgcag cgtgcgcgtg
     2221 gagcgacggc gcggtgtgcg ccgaaaacgg aattgagaac gcggccgcga gatgtccggc
     2281 cgcattgaag ccggtaaccc cggcgcagcg tgtcgcgtcc gcctgaagca catcgacggc
     2341 gccggcttcg agcatgcgcc ggaagtaaaa gctgtcgtaa ccgtactcgc ccgccgcgat
     2401 gcgcattcca gccggcg
//
LOCUS       DASZMC010000579         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4093012,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000579 DASZMC010000000
VERSION     DASZMC010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4093012"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VG274_11770"
     CDS             complement(<1..375)
                     /locus_tag="VG274_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWE07381.1"
                     /translation="MIRVAVIGAGAFGKNHVRVVGENPRAEIAMVIDKDPSRAPVSDF
                     RQAIGKVDAAIVAAPTVAHAEIACALLEAGIDVLIEKPIAATLEEADRIVEAAERNRR
                     IAQVGHLERFNPAILAVEKLATV"
     gene            complement(372..1184)
                     /gene="lpxI"
                     /locus_tag="VG274_11775"
     CDS             complement(372..1184)
                     /gene="lpxI"
                     /locus_tag="VG274_11775"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HWE07382.1"
                     /translation="MKYGIIAGNGRFPVLALETARRQGDEAVAFAIREEASPDVERAA
                     ARCYWISLGDLSRLIDTCKAEGIRQIMMAGQVKHARIFSAIRPDWRLLKVLAALPRKN
                     TDSLIGAVQKVLLDEGIELVDSTLLLKPLLAPAGAITKRKPDSDEQRDIDYGRKIAHM
                     LASMDLGQSVAICDKACVALEAMEGTDAMLRRAASLVEGKRLTLVKASSRRRHLLFDV
                     PAIGPGTVDTMIETGTGALAVDAGRTLMLDREELIAKANAAKLAIIGMEPTE"
     gene            complement(1181..1957)
                     /gene="lpxA"
                     /locus_tag="VG274_11780"
     CDS             complement(1181..1957)
                     /gene="lpxA"
                     /locus_tag="VG274_11780"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR01852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWE07383.1"
                     /translation="MPIHPTAIVDASARIDATAEIGPWCSIGPEVEIGARTRLMANLF
                     VEGPAAIGEDNVFFPYSTVGVASQDKKYKGERAFTRIGNRNSIREFVTIHRGTQGGGL
                     VTSIGDNCLLMTQSHVAHDCRIGNNVILGNSVGLAGHVTIEDWADLSPFTGVHQFCRI
                     GRHAFVGPYSVIKQDVLPFSLTSAQPEVQVFGANAIGLTRRGMEKSSVEALQTAFRLL
                     TRAGLNTSQAVERIRAEVPECDEVRELLEFIAASGRGVIK"
     gene            complement(1958..2422)
                     /gene="fabZ"
                     /locus_tag="VG274_11785"
     CDS             complement(1958..2422)
                     /gene="fabZ"
                     /locus_tag="VG274_11785"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWE07384.1"
                     /translation="MSILDVNEIRKILPHRYPFLLVDRIVELEPERIVGIKNVTVNEP
                     FFTGHFPDFPVMPGVLIVEAMAQTAGILVLRGIPDRDNKLVFLVSVDAARFRKPVVPG
                     DQLRLEMKVIKRKGSVAKMAGTATVNGVLVAEAEVMCKLEDKVPPQQETASV"
     gene            complement(2445..>3387)
                     /locus_tag="VG274_11790"
     CDS             complement(2445..>3387)
                     /locus_tag="VG274_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWE07385.1"
                     /translation="NESGIEGHGIVFTYTTAALEPTARFLRNIAPLAAGSDMRQLASR
                     FRLLGTQGLVGMALAGIDMALWDAHARAERKSLARLLGAEPKPLRSYGAVGYDGVAGS
                     ARVAEDWARRGFQGVKAKIGYPDVKEDLATIRAVRAAVGQDVAIMVDYNQSLTPEEAI
                     ARLRILESEGLTWVEEPVLAHDYAGHAAVARAIQTPIQSGENWWGPMDLSHAIDAGAS
                     DYVMFDAMKIGGPTGWIEASRMAQASGLPVSSHLWPELSAQLLSATPTAHWLEYADWW
                     NPIVLRPLRIEHGMAIPHDTPGSGVEWNEGAVAQYSI"
BASE COUNT          582 a         1036 c         1106 g          663 t
ORIGIN      
        1 cacggtggcg agcttctcga cggctagtat ggccggattg aagcgctcca gatggccgac
       61 ctgcgcgatc cgccggttgc gctcggcggc ttcgacgatg cgatcggctt cttcgagcgt
      121 ggccgcaatg ggtttctcaa tgaggacgtc gatgccggct tcgagcagcg cgcacgcgat
      181 ctcggcatgc gcgacagtgg gggcggcgac gatcgccgca tccactttgc cgatggcttg
      241 gcggaaatcg gaaacgggcg cgcgggaggg atctttgtcg atcaccatgg cgatttcggc
      301 gcgtggattt tcccccacca cgcgcacgtg attcttgccg aacgcaccgg ctccgatcac
      361 cgcgacgcga atcattcggt tggctccatt ccgataatgg cgagcttggc ggcgttggct
      421 ttcgcgatca actcctcgcg atccagcatg agcgtgcggc ccgcatccac tgcgagcgcg
      481 ccggttccgg tttcgatcat ggtatcgact gtgccgggcc cgatcgctgg cacatcgaaa
      541 agcagatgcc gccgccgtga cgaggccttc acgagtgtca gccgcttgcc ttccaccagg
      601 ctcgcggcgc ggcgcagcat ggcgtccgtc ccttccatgg cttcaagcgc cacgcaggct
      661 ttatcgcaga tggcgacgct ttgcccgaga tccatgctgg ccagcatgtg cgcgattttg
      721 cgtccgtaat cgatatcgcg ctgctcgtcc gaatccggct ttcgttttgt aatcgcgccc
      781 gccggggcga gcagaggctt cagcagcagg gtggaatcga ccagctcgat gccttcatcg
      841 agcagcactt tctgaacggc gccgatgaga gaatccgtgt tcttgcgcgg aagcgcggcg
      901 agcactttca gcaggcgcca gtcgggccgg atggccgaaa atatcctggc atgcttcacc
      961 tgcccggcca tcatgatctg ccggatgcct tccgctttgc aggtgtcgat gaggcggctt
     1021 aaatcgccaa gcgagatcca atagcagcgg gcggcggctc gctcgacgtc cggcgaggct
     1081 tcctcgcgga tcgcgaaggc aacggcttcg tcgccctggc gccgcgctgt ttcgagcgcg
     1141 aggacgggga accggccgtt gccggcgatg atcccgtact tcatttgata acgccacggc
     1201 cggaggccgc gatgaactcc agcagttcac ggacttcgtc gcactccggc acttccgcgc
     1261 gaatccgttc caccgcctga gaggtattca gaccggcgcg cgtgagcagg cggaaggcgg
     1321 tctggagcgc ttcgacggac gatttctcca tgcctcggcg cgtgagcccg atggcattcg
     1381 cgccgaacac ctgcacctca ggctgcgcgc tggttagcga aaacggcagc acatcctgtt
     1441 tgatcacgct gtaaggcccg acgaacgcat gacggccgat gcggcagaac tgatggacgc
     1501 cggtgaaggg gctcaggtcg gcccaatctt cgatggtgac gtggcccgcc agtccgacgc
     1561 tgttgccgag aatcacattg ttcccgatgc ggcaatcgtg cgccacgtgc gactgcgtca
     1621 tcaggaggca attgtcgccg atgctggtta cgagtccgcc gccctgcgtg ccgcgatgga
     1681 tagtgacgaa ttcacgtatg ctgttgcggt ttccgatgcg cgtgaaagcg cgctcgccct
     1741 tatacttctt gtcctgcgag gcgactccga cggtggaata ggggaagaag acgttgtctt
     1801 cgccaattgc ggcgggacct tcgacgaaga gattcgccat cagccgcgtg cgcgcgccga
     1861 tttcaacttc ggggccaatc gagcaccacg gtccgatctc cgccgtagca tcgatccgcg
     1921 ccgacgcatc gacaatcgct gtcggatgga tcggcattca gacggacgcg gtctcctgct
     1981 gcgggggaac cttgtcctcc agcttgcaca tgacttcggc ctcggctacc agcactccgt
     2041 tcacagttgc ggtgccggcc attttcgcga cgctgccctt gcgtttgatg actttcattt
     2101 cgaggcgcaa ctgatcgccc gggaccacgg gtttgcggaa gcgcgcggca tccaccgaga
     2161 cgagaaagac cagcttgtta tcgcggtcgg gaatgccgcg cagcacgaga atgccggcgg
     2221 tctgcgccat ggcttccacg atcaggacgc ccggcatgac ggggaaatcg gggaaatgcc
     2281 cggtgaaaaa tggctcgttg accgtgacat ttttgatgcc tacgatgcgc tcgggctcga
     2341 gttcgacgat gcgatcgacc agcagaaagg gataccgatg cggcagtatc ttccggatct
     2401 cgttcacgtc cagaattgac atggaagagt cagtataacg agcgtcagat cgaatattgc
     2461 gccactgccc cctcgttcca ttcgacgccg ctgccgggag tatcgtgcgg gatcgccata
     2521 ccgtgttcga tgcgcaacgg gcgaaggacg atcgggttcc accagtcggc gtattcgagc
     2581 cagtgggcgg ttggggtggc gctgagtaat tgcgcgctga gttcgggcca gaggtggctc
     2641 gatacaggca ggccgctcgc ctgagccatg cgcgacgctt cgatccatcc ggtgggtccg
     2701 ccgatcttca tggcgtcgaa catgacgtaa tccgaggcgc cggcgtcgat ggcgtgactg
     2761 agatccatcg gcccccacca gttttcgccg gactggatgg gggtctgaat ggcgcgcgcg
     2821 acggcggcgt gtccggcgta atcgtgggcg agaaccggtt cttcgaccca ggtcagccct
     2881 tcggattcga gtattcgcag gcgggcgatc gcttcttcgg gggtgaggct ctggttgtaa
     2941 tcgaccatga tcgcgacgtc ctggccgact gcggctcgca cggcgcggat ggtggcgagg
     3001 tcttccttca catccggata cccaattttc gctttgacgc cttggaatcc gcggcgggcc
     3061 cagtcttcgg cgactcgggc ggagcccgcg actccgtcat agccgacggc gccgtatgag
     3121 cgcagcggtt tcggctcggc tccgagaagg cgcgcgaggg atttgcgttc ggcgcgcgcg
     3181 tgcgcgtccc agagggccat gtcgattccg gcgagcgcca tgccgacgag gccctgagtc
     3241 ccgaggagac ggaaacgact cgccagctgc cgcatatccg agcctgcggc gagcggcgcg
     3301 atattgcgca ggaaacgggc ggtgggctca agcgcggcgg ttgtgtacgt gaagacaatg
     3361 ccgtgacctt cgatgcccga ttcgttg
//
LOCUS       DASZMC010000580         3845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3538243,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000580 DASZMC010000000
VERSION     DASZMC010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3538243"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VG274_11795"
     CDS             <1..208
                     /locus_tag="VG274_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07386.1"
                     /translation="RTTAAGFIDRSSAPFGEGRGAGLSTTCLTRPHAKEKFLWALPWR
                     PAWFRRISIIYQMLNYRKAIAHIS"
     gene            355..687
                     /locus_tag="VG274_11800"
     CDS             355..687
                     /locus_tag="VG274_11800"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWE07387.1"
                     /translation="MSSAAADRPFQDIEEPGRTGIVHKPEGLGVTYRSLAVNWNGVQG
                     NWKQLSGKMQERWGRLTHNNLRMMSGRRDRIIGRMQVSYGAAKEKAEHQLHMLKDLLE
                     RGKDATRH"
     gene            707..883
                     /locus_tag="VG274_11805"
     CDS             707..883
                     /locus_tag="VG274_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07388.1"
                     /translation="MNDRKPSGRTNWPSDAKSRVLPPVDARQGVALGHMRYVLGISVT
                     LAVVAFAVIYFAYF"
     gene            complement(887..1456)
                     /gene="efp"
                     /locus_tag="VG274_11810"
     CDS             complement(887..1456)
                     /gene="efp"
                     /locus_tag="VG274_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWE07389.1"
                     /translation="MVSVIASNVRKGNVLERDGKLYVVLTAENIHPGKGTPVTQLELR
                     RISDGVKVNERYKTTDTLEKAFIDERTHTYLYHDGNHYIFMDPQTFDQISVLESILGD
                     SAPFLVENMDVRLSLYNGIPISIELPARVTLEVAETEPVMKGQTASGSFKPATLSNGA
                     RTMVPTHITAGTRIVVLTADGSYVERAKD"
     gene            1521..2564
                     /gene="epmA"
                     /locus_tag="VG274_11815"
     CDS             1521..2564
                     /gene="epmA"
                     /locus_tag="VG274_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HWE07390.1"
                     /translation="MPQWWTAHVHADRRATLQARGRIKNALRHYFDNRGFTEVECGIL
                     QVSPGNEAHLHAFATEWIAPAAAGERLYLHTSPEFACKKLLSAGEKQIFDFARVFRNR
                     ERSALHAPEFTMLEWYRAEDTYCAVMSDCVEMLKLAAGAVPAQRLAFRGRTCDPFLDP
                     ERLTVAEAFRRFAGIDLLATLSDEGEGDRDALAEAARGAGVDAAIDDTWSDLFSKLLV
                     ARIEHRLGLERATILHEYPRCEAALARATPHDARLAERFELYACGVELANGFGELTDP
                     AEQRRRFEAEMKEKQRIYGERYPLDEGFLAALSSMPDASGVALGFDRLVMLALGAPRI
                     DDVIWTPLPEPRS"
     gene            2561..3154
                     /locus_tag="VG274_11820"
     CDS             2561..3154
                     /locus_tag="VG274_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07391.1"
                     /translation="MSVEHARAQTPSGIAGIRDLQESDIQDVVTYWHESSAEHLDDLG
                     IDRDRLGSRGSTYARFHQALRSGDPDQTNIAFAVTLDDEFVGYTLLNRYDPQINYSHW
                     HITNLRRRAAGLSTALYPHRIKTYFDTTPIARLIHQTRTRNSGVNRMLDKYLPVAETQ
                     FIEHPDGVAKPGEFHIRYAFRESVPRLFEIAAGLMKI"
BASE COUNT          720 a         1194 c         1194 g          737 t
ORIGIN      
        1 gcgaaccaca gctgctggtt ttattgatag gtcgtcggcg ccatttgggg aaggcagggg
       61 agccggcttg agcacaacgt gcctgacccg cccccacgca aaagagaaat ttctttgggc
      121 gctcccgtgg cgaccagcct ggttcagaag aatatccatt atttatcaga tgcttaacta
      181 cagaaaagcg attgcgcata tttcttgaaa aagatttgga acccaatgta gaccccggcg
      241 ttctattccc cgagcggatg tccgatccgg tacgccccag ccccttcgac accccgcatc
      301 gggtgtccgc tcgcttaact caaaagccgc tcctcgggag cggttttttt gtttatgtca
      361 tctgccgctg ccgatcggcc cttccaggat attgaggaac cgggccgcac agggatcgtt
      421 cacaagccag aagggctcgg cgtcacatat cggagcttgg cggtgaattg gaacggggtt
      481 cagggcaatt ggaagcagtt gtccggcaaa atgcaggaac gatggggtcg gctgacgcac
      541 aacaacctgc gtatgatgtc aggccggcgc gatcggatca tcggccggat gcaggtaagc
      601 tacggcgccg caaaggaaaa agccgaacac cagttgcaca tgctcaagga tttgctggaa
      661 cgcgggaagg acgcgacgcg gcactgactg cgccacgcga acgacgatga acgaccgcaa
      721 accgtccgga cgcaccaact ggccgtcgga cgcgaagtcc cgggtgctgc cgcccgtcga
      781 cgcgcgccaa ggtgtggcgc tcgggcatat gcgctatgtt ctcggaatca gcgtgacgct
      841 tgccgtggtg gctttcgcgg tcatctactt cgcgtatttc tgactctcag tccttggctc
      901 gctcgacgta ggaaccatcc gccgtcagca cgacgattcg cgttcccgcc gtgatgtgcg
      961 tcggcaccat cgtgcgcgca ccattggaaa gggtggccgg cttgaaagag ccggacgccg
     1021 tctggccctt catcacaggt tccgtttcgg cgacctcgag cgtgacgcgc gcaggcagct
     1081 cgatcgaaat gggaatgcca ttatagagcg acagtctgac atccatattc tcgacgagaa
     1141 agggcgcgga atctccaagg atgctctcga gcacggaaat ctgatcgaag gtttgggggt
     1201 ccatgaaaat gtagtgattg ccgtcgtgat agagataggt gtgcgtgcgc tcgtcgatga
     1261 atgccttctc gagcgtatcg gtggtcttgt agcgctcgtt gaccttcacg ccgtcgctga
     1321 tccggcgcag ttccagctgg gtgaccggcg tgccctttcc gggatggatg ttctccgcgg
     1381 tgaggacgac gtagagcttg ccgtcgcgct ccagtacgtt gcccttgcgc acattgcttg
     1441 cgataaccga aaccaccgaa cgaccttccg cgcgtgtcgc gcggggtcat agcagccgcc
     1501 tgaggtcggg ccaagagccg atgccgcaat ggtggaccgc gcatgtccat gccgaccgca
     1561 gggccaccct tcaggcgcgg ggccgcatca agaatgcgtt gcgccactac ttcgacaacc
     1621 ggggcttcac ggaagtggag tgcggcatcc tgcaggtctc acccggaaac gaggcgcatc
     1681 tgcatgcgtt tgcgaccgag tggatcgcgc ccgccgccgc cggcgagcgt ctctatctgc
     1741 atacgtcgcc cgaattcgcc tgcaagaagc tcctctcggc aggcgagaag caaatcttcg
     1801 atttcgcgcg cgtgttccgc aaccgcgagc gcagtgctct gcatgcgccg gaattcacca
     1861 tgctcgaatg gtatcgggcg gaggacacct attgcgcggt gatgagcgat tgcgtcgaga
     1921 tgctcaagct tgccgcaggc gccgtgcctg cgcagcgtct cgcttttcgg ggacgcacct
     1981 gcgatccgtt tcttgatcca gaacgattga cggtcgccga agcgtttcgg cgctttgctg
     2041 gcatcgacct gctcgccacg ctgtcggatg agggcgaggg cgaccgcgac gctttggccg
     2101 aagccgcacg gggcgctggc gttgatgcgg ccatcgacga cacctggtcc gatctgttca
     2161 gcaagctcct tgtcgcgcgc atcgaacatc gtctgggact ggaacgcgcg acaattctcc
     2221 atgaataccc gcgctgcgag gcggcgctcg cacgcgcaac accgcacgat gcacgactcg
     2281 ccgaacgttt cgagctttat gcctgcggcg tcgagctcgc gaacggcttc ggcgaactga
     2341 ccgatcccgc cgaacagcgc cggcgcttcg aagcggaaat gaaggagaag cagcgcatct
     2401 atggcgagcg ctacccgctc gacgaggggt ttcttgcggc gctgtcgagc atgccagacg
     2461 cgagcggcgt ggcgctgggc ttcgaccgcc tcgtcatgct ggcgctcggc gcgccgcgga
     2521 tcgatgacgt catctggaca ccattgccgg agccgcgaag ttgagcgtgg aacatgcgcg
     2581 cgcgcaaacg ccgtccggca tcgcgggaat acgggatctg caggaatcag atattcagga
     2641 tgtcgtcacc tattggcacg agagcagcgc ggaacatctc gatgatctgg gcatcgacag
     2701 agatcgcctc ggcagccggg gcagcaccta cgcgcgcttt catcaagcac tccgatccgg
     2761 cgatcccgac cagacgaaca tcgcctttgc cgtcaccctc gacgacgaat ttgtcggtta
     2821 cacgctgctc aaccgctacg acccgcagat caactattcc cattggcaca tcaccaattt
     2881 gcggcgccgc gcggccggtc tttcgacggc gctttatccg catcgcatca agacctattt
     2941 cgacaccacg ccgatcgcgc ggctgatcca ccagacgcgc acgcgcaaca gcggagtgaa
     3001 ccgcatgctc gacaaatatc ttcctgtcgc cgagacccag ttcatcgagc atccggatgg
     3061 cgtcgccaaa cctggcgaat tccacattcg ctatgcgttt cgcgaaagcg tcccccgcct
     3121 cttcgaaatc gcggcgggct tgatgaagat ctgatgtgga tgcgatccgc gcgggcgtta
     3181 tcggccttgc gtccgcgcgc tgaagcgatg ccgggccgcg cgcggtgggc aagattcccc
     3241 gcgcgcgccc tcggcatctt cagtggctgg gcggctgccc ctgttctcct ggcggtggac
     3301 cttcttcacc cggaggatag gcaccctgct gctgtagtct ggcgcatttg cgggggtgct
     3361 tggcacacca atttggcccg tggcgatggg gcggagcgcc ggcctcgccg tccgcataac
     3421 cttcctctcc cggtggagcg ccataagcct gcccttgcgg tggcggtcca tagccttgcc
     3481 cctgcggcgg gggtccgtaa ccttgctcct gcgggggcgg gccatagcct tgtccgggag
     3541 caggtccgcc ctcttctgca ccaggcggcg gtccataagc tccgggaggc ggaccctgct
     3601 cgtagggttc gtagggtcca tagggataaa cgcaaccgcc aagcgtcacg gccgctccaa
     3661 gcaatgcggc tgatatcagt agggaacgca tgatctagtc ctcaacgagg agaggagccc
     3721 atccgggggc catctaaggc gatgcatgcc gtcggcgcaa ccggggcggc cccatcctct
     3781 atccgggcgc cgtgttcaag agtggccgag acaacggcct ggccgtctat gaacatcgcg
     3841 tcatg
//
LOCUS       DASZMC010000581         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1878576,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000581 DASZMC010000000
VERSION     DASZMC010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878576"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VG274_11825"
     CDS             complement(<1..434)
                     /locus_tag="VG274_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07392.1"
                     /translation="MTSSARIFDKRTGLLFVVAFGVVSLFADMAYEGMRAISGPFLAT
                     LGASGAAVGAIAGGGEMAGYLLRLVSGRAADRTRLYWPITLAGYIMQMAAVPLLALAG
                     NWWMAAVLIVAERAGKAVRNPPRDVMLSRAGEEIGQGWAFGL"
     gene            complement(651..>3008)
                     /gene="pheT"
                     /locus_tag="VG274_11830"
     CDS             complement(651..>3008)
                     /gene="pheT"
                     /locus_tag="VG274_11830"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWE07393.1"
                     /translation="ETDADARTIAETLTNIGLEVESLEDRGAALRDFIVAHVVSAEKH
                     PNADKLKLCRVDTGSDIVQVVCGAPNAHTGMKGVFARPGLVIPHSGETLKISSIRGIE
                     SRGMLCSERELLLSEEHEGIIELDPAAEIGTPAARVLGLDDPVFDVAITPNRGDCTSV
                     AGIARDLAAAGVGHLKNAEVASVKGAFPSPFGIALHFPSGAEDACPIFAGRFIRNVRN
                     GPSPAWLQTRLRAVGLRPISALVDVTNFVANDRGRPLHVFDADKLAGGMQARLARNGE
                     IVTALDGKTYTLDPEMIVIADDNRALSIAGVMGGEDTGCTETTRNVFIESAWFDPRRI
                     AATGRKLGIQSDARYRFERGTDPEFVVPGLELATRLILEFCGGEPAETVVAGSPPPWR
                     REIAFSARHVRRLAGINLTSGDIGKILVSLGFAVEGEREMRVTPPSWRSDIAGSADLV
                     EEVVRIHGLDRIPAAPMTRAHAVARAVLTPSQKRTRLARRTLAARGFNETISYSFIPR
                     AHAALFGGGDELRQLENPIAADLDALRPSVLPSLLSACARNAARGFPEQSLFEIGAQF
                     ESGMPGAQMLVAAGVRLGPAPRSWTRDTHHADAFDAKADMLAVLESAMGGTMTAPVKS
                     GAPGWYHPGRSATLGLGTKTLAQFGELHPAVLEAFDLKGPASAFELFLDAVPEPKARS
                     RTRAAFHPSQFPAVERDFAFVVDAKVTAEDIVRSARNAERVLIERVDVFDVYEGAGVP
                     EGKKSLAISVRLQPRDHTLTDAEIETVAQKIVTAVAKATGGTLRT"
BASE COUNT          542 a         1001 c          967 g          498 t
ORIGIN      
        1 tgcaatccga aggcccagcc ctggccaatc tcctcgccag cgcgcgacag catcacatcg
       61 cgcggcggat tgcgcaccgc cttgccggcg cgttcggcca cgatcagcac cgccgccatc
      121 caccagtttc ccgcgagtgc aagcaacggc acggccgcca tctgcatgat gtagccagcg
      181 agcgtgatcg gccaatagag ccgcgtgcga tccgcagcgc ggcccgaaac gagccggagc
      241 aggtagcccg ccatctcgcc gccccccgcg atggcgccga ccgccgcgcc gctcgcgccg
      301 agcgtggcaa gaaacggtcc agagatcgcg cgcatgccct cataggccat gtcggcgaac
      361 aggctcacca cgccgaacgc gacgacgaag agaaggccgg ttcgtttgtc gaaaatgcgc
      421 gcggaggagg tcatgcgaaa cactctaacc catccgtttc cccctcacct gcgcctgagc
      481 ggttgataga cctcgtattt gagccctctc ccacaagggg agaggggaca ccgcgcgctt
      541 atcaagagtg gctgatgcag cactccgatt cccctctccc cttgcgggag aggcatgcag
      601 gtacaggcgt tgaaaccatt cgggcctggg tgagggggaa cacgggcgga tcaggtccgc
      661 aatgtgccgc cggtggcctt ggcgacggcg gtaacgatct tctgggcgac cgtttcgatt
      721 tcggcatcgg tcagcgtgtg gtctctgggc tgcaggcgca ccgagatggc gagcgatttc
      781 tttccttcgg gaacgcccgc gccctcatag acatcgaaaa catcgacgcg ctcgatcagg
      841 acgcgttccg cattgcgcgc gctgcgtaca atgtcttccg ccgtaacctt cgcatcgaca
      901 acgaaggcga aatcgcgttc cacggcgggg aattgcgaag gatggaacgc ggcgcgggtc
      961 cggctccttg ccttcggctc gggaacggca tcgaggaaca actcgaatgc cgaagccggg
     1021 cctttcaggt caaatgcttc gagaaccgcc gggtgcaatt cgccgaattg ggcgagcgtc
     1081 ttggtgccga gcccgagcgt ggcggaacgt ccggggtggt accagcccgg cgcgcctgac
     1141 ttcaccggcg cggtcatcgt tccacccatc gcggattcga gcaccgccag catgtcggcc
     1201 ttcgcatcga acgcgtcggc atggtgcgtg tccctggtcc agcttcgcgg tgccggccca
     1261 agccgcacgc ctgccgcgac aagcatctgc gcgcccggca taccgctttc gaactgcgcg
     1321 ccgatctcga acagactctg ctccgggaaa ccgcgcgcgg cgttgcgagc gcacgccgaa
     1381 agaagcgaag gaagaacgct tggccgcagt gcatccaagt cggccgcaat gggattttcg
     1441 agctggcgca attcgtcgcc gccgccgaac aaagcggcgt gcgcgcgcgg aatgaacgaa
     1501 taggaaatcg tctcgttgaa gccgcgcgcc gccagcgtac gtctcgcgag gcgcgtccgt
     1561 ttctgcgatg gcgtcagcac cgcccgggcg acggcatggg cgcgcgtcat cggcgccgcg
     1621 ggaatccggt caagtccgtg aatgcgcacc acttcctcga ccagatcggc ggaaccggcg
     1681 atgtcgctgc gccaggacgg cggcgtcacg cgcatctcac gttcgccttc aacggcgaag
     1741 cccagcgaca cgagaatttt gccgatgtcg cccgatgtga gattgattcc ggccagccgg
     1801 cgcacatgcc gcgcggaaaa cgcaatttcg cggcgccatg gaggtggact gccggcgacc
     1861 acggtctccg ccggctcgcc gccgcagaat tcgaggatca atcgcgtcgc cagttcaaga
     1921 ccgggcacca cgaattccgg gtcggtgccg cgctcgaaac ggtagcgcgc atccgactgg
     1981 atgccgagct tgcggcccgt cgcggcaatc cgccgcggat cgaaccaggc cgattcgatg
     2041 aacacgttgc gcgtcgtctc cgtacagccc gtgtcctcgc cgcccatcac gccggcaatg
     2101 ctgagcgcgc ggttgtcgtc ggcgatgacg atcatctccg gatcgagagt gtaggtcttt
     2161 ccatccagcg ccgtcacaat ctcgccgttg cgcgcgagcc gcgcctgcat gccacctgcg
     2221 agcttgtccg catcgaacac atgcaacggc cgcccgcgat cgttggcgac gaaattggtc
     2281 acgtcgacga gcgccgagat cggccgcagt ccgaccgcgc gcagccgggt ttgcagccac
     2341 gccgggctcg gcccgttgcg cacattgcgg atgaaccgtc cggcgaagat cgggcacgca
     2401 tcttcggcgc ccgacggaaa atgcagggcg atgccgaacg gcgacggaaa tgcacctttc
     2461 acagatgcga cttcggcgtt tttcaaatgt ccgacacctg ccgcagcgag atcgcgcgcg
     2521 atgccggcca cgctggtgca gtcgccgcgg ttgggggtga tcgcgacgtc gaacacggga
     2581 tcgtcaaggc cgagcacgcg cgctgccggt gtgccgattt cggcggcagg atcgagttcg
     2641 atgatgcctt cgtgctcttc ggagagcaga agctcgcgct ccgaacagag catgccgcgc
     2701 gattcgatgc cgcgaatgct ggagatcttg agcgtctcgc ccgagtgcgg aatgaccaga
     2761 ccgggccgcg caaacacgcc tttcatgccg gtatgcgcgt tgggcgcgcc gcatacgacc
     2821 tgcacgatgt cgcttccggt atcgacccgg caaagcttga gcttgtcggc gttgggatgc
     2881 ttctcggccg agacgacatg ggcgacgatg aaatccctca gcgccgcgcc gcgatcctcc
     2941 agcgattcca cttcgaggcc gatattggtg agcgtctcgg cgatagtgcg cgcatccgcg
     3001 tcggtctc
//
LOCUS       DASZMC010000582         7575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_239645,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000582 DASZMC010000000
VERSION     DASZMC010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239645"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VG274_11835"
     CDS             <1..718
                     /locus_tag="VG274_11835"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWE07394.1"
                     /translation="ILASHSSVEGVGELPNIRFIARYLQDRVAPQRGTDFPGVLGELD
                     ASALKALGEEYLEGAGARRRLGRPLFVDKEPFNFWHVGFIQLILPHARIVDVRRHPLG
                     CCFSNFTTIFLHGLALTTRLADLGRYYADYVELMAHFDRVLPGRVHRIFYERLVAEPE
                     TEIRALLDYLELPFEKGCLEFHRNARAVNSASSEQVRSPIYDDALDHWRNYEPWLGPL
                     KSALGPVLEAYPWSGTTARP"
     gene            complement(758..994)
                     /locus_tag="VG274_11840"
     CDS             complement(758..994)
                     /locus_tag="VG274_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07395.1"
                     /translation="MGQSIEPQSPEQRIVHYRDMAMEVIRLSGETHSAGMKAQFLQLA
                     QSWLALADEVARNIPEAEATAVRATFTLDATLPA"
     gene            complement(1155..3377)
                     /locus_tag="VG274_11845"
     CDS             complement(1155..3377)
                     /locus_tag="VG274_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWE07396.1"
                     /translation="MDIAATDMKQERFVLGLPVVCARNALIFAVAAIAFTFSTAQDAA
                     PAAAPASTSVAALDPFLAGAGHLGNIGLAAFLAGNPNLAAYAANGLSADGASAAIVLF
                     ETTSGSSVTFQINSAATLLPYTDNFLTTAPTAGQSSQTVSSLIQVGSEYYAPVLVQGP
                     LAGYSQDNTISITATQDGAQAGAKLALTIPPLVLVHGLWGDRKSLSQAETWLRSHAPW
                     KSAPALVAPICYSKYLRFDAKTDPLSNGNDPCEVTSKASLQTEIDSLMAELDGEQIVG
                     ARVDLVVHSMGGLVARNYASQAKYGSLRNRMQGQFHAIVTLNTPETGSLLAPFLIAKR
                     DAKRKAPLWTPQGFIWNAVCGNATVARCFDANGYPIYAPSLRVDSGAVYSLDPDGPSL
                     NNPDLSGPNIGNATWRAIGSTKPGNSALALGLDPLIAALYRNPDSNNVPTVDSILQDE
                     PNDAIVTVDSQTKGAEGNQFYTFARLSHTSLVSSILTWLTRNNINDNSVTDDPSHGVD
                     KLAACWIETTGSDSCLGLRALIAPGAEAAPTSPRMLKPVDRMAVRGPTRVTLGTPFVA
                     AIRVPPSGLRPQLAVYQLGETGRAKPEEVTATGANGDLHVRITPKLLGPVSFGVRAVF
                     ADGGVSVQTVHAFVAPPAAPPLAFRANELPVLVLTRDSDTATAMPHPAALYPAPIGWL
                     DLNARFVTWTLVPQPGAPTIRVDPNGLLHALAPGDASVEAHFGSVTAPLRVIVRQTQE
                     "
     gene            complement(3567..4751)
                     /locus_tag="VG274_11850"
     CDS             complement(3567..4751)
                     /locus_tag="VG274_11850"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWE07397.1"
                     /translation="MPAAAGPKHPFAMPRLDHVWVIMMENHGYVQIINNPYAPFANQE
                     AQSANLAANYFAVAHPSLTNYLEVIGGSNFGVTDDNSPDWHNSSCQPNIVSGVNNNEA
                     DPNPICPIAGSGTDQPTPALDCSNEVNGCPPGPVINIDGIESYSAAATVGAMIGDQLA
                     AAGKSWKSYQESLPPGGADGVDYGDGQFSNLTNFTQAEISLGETTGAEVQLYAAKHNP
                     FAYFASVQSGTIAGDTLANSVDFATLYGDLATNKAPALSFIAPNQCNDQHGRGNAGPF
                     CAFDPNDNGTQTGLNPALIQQGDQSLQKIVTAIKASPVWGKGHNAIVILWDEDDYSVS
                     PTTNQVALIVDTNFAKKRVTSNNFYNHFNLTNTLEAAFGLPCLNHACDPQSYPMADLF
                     QR"
     gene            5400..6491
                     /locus_tag="VG274_11855"
     CDS             5400..6491
                     /locus_tag="VG274_11855"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWE07398.1"
                     /translation="MSIASNHTVDGVAPDVRGLVRSFLRYREPDLSRALFELGVTAGA
                     LLLGWGLMWLSLATGYWLTLLLAIPTAGFLVRLFMIQHDCGHGAFFPRRAANDWIGRA
                     LGIFTLTPYDDWKRSHTVHHATSGNLDRRGIGDILTLTVDEYSSRSMLGRLAYRAYRH
                     PLVMFGFGPAWLFLLRQRFPFEQMRWSWRPWASTTATNAGIAVLAGLGIWLIGFGPFA
                     AVQLPVMLLAGSIGVWLFYVQHQFENVAWARDGDWSVSGAALSGSSHYVLPSVLRWFT
                     ANIGVHHVHHLCSRIPYYRLDRALRDHPQLETLGRVTLGQSLRSVNLVLWDDTRKRLI
                     SFREAALARRSRECAARAGGMVRRDACPV"
     gene            6827..7507
                     /locus_tag="VG274_11860"
     CDS             6827..7507
                     /locus_tag="VG274_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07399.1"
                     /translation="MNWIFWAAPLLVAISGLALLSSGIGHLLRGRAVRASGHLVFGAP
                     LAVIGFVAGLLGLNAQTYARLTHEGPVAQVSVRRLDPASARWLVTIRRLDGTNRIQTC
                     ELQGDEWVLSGRVQKWKPWANLLGLDATYTLEQLSNMYFTAQRGNGRPITACDIADVA
                     PRIDRYLPPSWLAWLAARSFAVDRRFGSANYMPLADAAVYRVVITQSGFNAEPVNPAA
                     HAANEARP"
BASE COUNT         1301 a         2348 c         2474 g         1452 t
ORIGIN      
        1 gattctcgca agccattcct ccgtcgaagg ggtgggcgag cttcccaata tccggttcat
       61 cgcgagatat ctgcaggatc gcgtcgcacc gcaacggggt acggattttc cgggcgtgct
      121 gggcgaactc gatgcgtccg cactgaaggc gctgggcgag gaatatctcg aaggcgcggg
      181 cgcccgcagg cgcctggggc gtccgctctt cgtcgacaag gagccgttca atttctggca
      241 tgtggggttc attcagctga tcctgcccca tgccaggatc gtcgacgtcc ggcgccatcc
      301 gctcggctgc tgcttctcca acttcaccac catcttcctg catgggctgg cgctgaccac
      361 ccggctcgcg gatcttggcc gctactacgc ggattacgtc gagctgatgg cgcatttcga
      421 ccgcgtgctg cccgggcgcg tgcaccgcat cttctatgag aggctggtag cagagcccga
      481 aacggagatc cgcgccctgc tcgactatct cgaattgccg ttcgagaaag gctgtctcga
      541 attccatcgc aacgcgcgcg cggtgaactc ggccagctcc gagcaggtgc gctcgccgat
      601 ctacgacgac gctctcgatc actggcgcaa ttacgaaccc tggctcggcc cgctcaaatc
      661 ggcgctcggc ccggtgctcg aggcctatcc atggtccggg acaacggccc ggccatgacg
      721 agatttggtg tgcttccggt cggcgggagc ggcttcgcta agcggggagc gtcgcgtcca
      781 acgtgaacgt ggcccgcacc gcggttgcct cggcttcagg gatattgcgc gcgacttcgt
      841 cggccagcgc aagccacgac tgggccagct gcaggaactg cgccttcatt ccggcggagt
      901 gggtctcgcc cgatagcctt atgacttcca tcgccatgtc gcggtaatgg acaatccgct
      961 gttccggcga ctgcggctcg attgactggc ccataggcgc acttgtccct ctcgtccccc
     1021 cgaactcacc gaagcgccaa cgaagaacag tgcaagccgt tcccgcaatt cacagattcc
     1081 cgttcgtgcg tgacaatatt gcaatcccgc gacggcaatt tgggaaagcg gcaagccgaa
     1141 aggtgtgccc cgatctattc ctgggtctgg cgcacgatca cgcgcagggg ggcggtcacc
     1201 gagccgaaat gcgcttccac gctcgcgtcg cccggtgcaa gcgcgtgaag gagaccgttc
     1261 ggatcgacac ggatcgtggg tgcgccgggt tgcggcacca gcgtccaggt cacgaagcgg
     1321 gcattgaggt cgagccagcc gatgggcgcc gggtagaggg cggccggatg cggcatggcg
     1381 gtcgcggtgt ccgaatcgcg cgtcagcacc agaaccggca gttcgttggc gcggaaggcc
     1441 agcggcggtg cggccggcgg cgcaacgaag gcgtgcactg tctgcaccga tacgccgcca
     1501 tcggcgaaaa ccgcgcggac gccgaagctc accggcccaa ggagtttcgg cgtgatgcgg
     1561 acatgcaaat ccccgttcgc acccgtcgcc gtcacctctt ccggcttcgc gcgccctgtt
     1621 tcgcccagct gatacaccgc gagctgcggg cgcaaaccgg acgggggcac gcggatcgcg
     1681 gctacgaagg gcgtaccgag cgtgacgcgc gtggggcccc gcacagccat gcggtccacc
     1741 ggcttcagca tgcgcgggct tgtcggcgcg gcctcggcgc ccggcgcaat gagtgcccgc
     1801 aagccgaggc aggaatccga tccggtcgtc tcgatccagc aggccgcgag cttgtccacg
     1861 ccgtgcgacg gatcgtcggt cacgctgttg tcgttgatgt tgtttcgggt cagccaggtc
     1921 agaatgctgc tgacgagcga ggtgtgagag agcctcgcga acgtataaaa ctgatttccc
     1981 tcggcgcctt ttgtctggct gtcgacggtc acgatggcgt cgttcggctc gtcctgcaga
     2041 atcgaatcca ccgtcggcac gttgttggaa tccggattcc tgtagagcgc cgcgatcagc
     2101 ggatcgagcc ccagcgcgag cgcgctgttg ccgggcttcg tcgagccgat cgcgcgccac
     2161 gttgcgttgc cgatattggg gccggacagg tcgggattgt tgaggcttgg tccgtcggga
     2221 tcgagcgaat aaacggcgcc gctgtcgacg cgaagggaag gcgcgtagat cggatagccg
     2281 ttcgcgtcga agcatctggc aaccgtcgcg ttgccgcaaa ccgcgttcca gatgaatccc
     2341 tgcggcgtcc acagcggcgc cttgcgtttc gcatcgcgct tggcgatcag gaacggcgca
     2401 agcagcgaac ccgtctccgg cgtgttcagc gtgacgatcg cgtggaactg gccctgcatc
     2461 ctgttgcgga gcgagccgta tttcgcctgc gaggcgtagt tgcgggcgac gaggccgccc
     2521 atgctgtgca ccaccagatc cacgcgcgcg ccgacgatct gttcgccgtc cagctcggcc
     2581 atcagcgaat cgatttcggt ctgcagcgag gctttcgacg tcacttcgca ggggtcgttg
     2641 ccgttgctca gcggatccgt cttcgcgtcg aaacgcagat atttcgaata gcagatcggg
     2701 gccacgagcg cgggcgcgct tttccagggc gcatggctgc gcagccaggt ttccgcctgc
     2761 gacaggcttt tgcggtcgcc ccacaggcca tgcacaagca cgagaggcgg gatggtcagg
     2821 gcgagtttcg cgcccgcctg cgctccgtcc tgggtggcgg tgatgctgat cgtgttgtcc
     2881 tgcgaatagc ccgcaagcgg cccctggacg aggacgggcg cgtaatattc cgaaccgacc
     2941 tggatcaggc tggacaccgt ctggctggat tgtccggcgg tgggcgccgt cgtcaggaaa
     3001 ttatccgtgt acggaagaag cgtcgcggcg ctgttgatct ggaaggtgac ggaggaaccg
     3061 gacgtggtct cgaagagcac aatggcggcg gaggcgccgt cggcggaaag tccgttggcg
     3121 gcataggcgg cgagattcgg atttcccgcg agaaaagccg caagaccgat attgccgaga
     3181 tgccccgcgc cggccagaaa cggatcgagc gcggccacag acgtcgaagc aggagcggca
     3241 gccggcgccg cgtcctgcgc cgttgaaaat gtgaaggcga tcgccgcaac cgcaaatatc
     3301 agcgcgttgc gcgcgcacac gacgggcaag cccaggacga atctttcctg cttcatgtcg
     3361 gtcgccgcga tgtccacggc aggttagcac gacaggacgt taacagttgc gtaaagcgcg
     3421 tcgtacggtg cgtccgtttc gggcgcgtgc gcaccatagc cgggagcagg tgctttgcaa
     3481 tcgtcaatct cccggcgcgt tcatggcaaa gccggtcggc caggggtgtg gtgccccctg
     3541 gccggcctcg atgcgcgccg gaacggctaa cgctggaaaa gatccgccat cggataggat
     3601 tgcgggtcgc atgcatggtt caggcagggc agcccgaacg cggcttcaag cgtgttcgtc
     3661 aggttgaagt gattgtagaa attattgctg gtgacgcgct ttttggcgaa attggtgtcg
     3721 acgatcagcg cgacctggtt ggttgtgggg ctgacgctgt aatcgtcctc gtcccacaag
     3781 atgacgattg cattgtggcc tttgccccat accggggagg ccttgatcgc cgtgacgatc
     3841 ttctgcagcg actgatcgcc ctgctggatc agtgcggggt tgaggccggt ctgcgtgcca
     3901 ttgtcgttcg gatcgaaggc acagaacgga ccggcatttc cacggccgtg ctggtcgttg
     3961 cactggttgg gcgcgatgaa cgagagggca ggcgctttat ttgtcgccag atcgccatac
     4021 agcgtcgcga aatccacgct attcgccagc gtatcgcccg caatcgtgcc gctctgcacg
     4081 ctggcaaaat aagcgaacgg attgtgcttc gccgcgtaga gctgcacttc cgcgccggtc
     4141 gtctcgccca acgagatttc ggcctgggtg aaattggtga ggttcgagaa ctggccgtca
     4201 ccatagtcga cgccgtccgc gccgcccggc ggcaggctct cctgatagga cttccagctc
     4261 ttgccggcgg cggcaagctg gtcgccgatc attgcgccga cggtggcggc cgcgctgtaa
     4321 ctttcgatgc cgtcgatgtt gatcaccggg cccggaggac agccgttcac ctcgttgctg
     4381 caatccagtg ccggggtcgg ctggtcggtg ccgctgccgg caatcggaca aatcggattc
     4441 gggtccgcct cgttattgtt gacgccgctg acgatattcg gctggcagga ggagttgtgc
     4501 caatccgggg agttatcgtc ggtgacgccg aaattggagc cgccgatcac ttccagatag
     4561 ttggtcaggc tgggatgcgc cacggcgaag taattcgccg ccaggttcgc cgattgcgcc
     4621 tcctgattgg cgaacggcgc gtatgggttg ttgataatct gcacgtagcc gtggttttcc
     4681 atcatgatga cccagacatg gtccagccgc ggcatggcga acggatgctt cggaccggcg
     4741 gcagccggca agacagcagc gatcgcagcg ccggcgagca gcgccagcgc agtgtgcttc
     4801 aatctcatag tggttcccct tgaagaatgt ggttaggcgg gcagagtcac tcgcccgccc
     4861 gtattaggga cttcgctctg ttgcagcgga tggaaaattt tgcgacacct gcgtgatgat
     4921 tgcgtgacgc attgatgatg gtaggcgaag ccggacacac gcggcctctg gctggtggat
     4981 aggcgccaag ataacccagg ccgaaagacc cctcacccgc gcccgagcag aaatcattgc
     5041 acgtatctgc atgcctctcc cacaagggga gaggggaaaa cggagtcttg catcgaagtc
     5101 gggaaatcag cgcgtggagt ctcctctccc ctcgcgggag aggggaaaca ttcgagtcct
     5161 cgctggcgtt ccgaaatggg tgaggggccg ctcgggctgc gttaacccgg cgcaaaaccg
     5221 cagggcgcgg ttgcattccg gatggcgtcg tgggacttgg atcgcgtcgc ggccaacatt
     5281 catgcgatgg ccagatcgta gatcgccact acattcctgt gagcccttta gtacagattt
     5341 cgcaatgatt aagggcgata ctccacggcc aaccacgaac tttcaaaggg atagagagct
     5401 tgagcatcgc atcaaatcac actgtggacg gagtggcgcc ggatgtgcgc gggcttgttc
     5461 ggagcttcct gcgatatcgc gaacccgacc tgtcgcgggc cctgttcgag ctcggcgtga
     5521 ccgccggcgc cctgctgctg ggctggggac tgatgtggct ctctctcgca accggctact
     5581 ggctgacgct gctgctcgcg atcccgacgg ccggattcct cgtccggctg ttcatgattc
     5641 agcacgattg cgggcacggc gcatttttcc cgcggcgcgc cgccaatgac tggatcggac
     5701 gggcgctcgg aatattcacg ctcacgccgt acgacgactg gaaacgctct cacaccgttc
     5761 atcacgccac gtccggcaat ctcgaccggc ggggaatcgg cgacatcctg actctcaccg
     5821 tggacgagta ctcgtcgcga tccatgctcg gccggctcgc ctatcgcgcc tatcgccatc
     5881 cgcttgtcat gttcggcttc ggacccgcct ggctgtttct cctgcggcaa cggtttccgt
     5941 tcgagcagat gcgctggagc tggcggccct gggcaagcac cacggcgaca aacgccggga
     6001 tcgcagtgct ggccggcctc gggatctggc tgatcgggtt cgggccattc gcggcggtgc
     6061 agctgcccgt catgcttctc gccgggtcga tcggcgtctg gctgttctac gtgcagcacc
     6121 agttcgagaa cgtcgcgtgg gcgcgcgacg gcgactggtc ggtctccggc gcagccctgt
     6181 cgggaagctc gcattatgtt ctgccgtccg tgctcagatg gttcaccgcg aatatcggcg
     6241 tgcatcatgt gcatcatttg tgcagccgaa tcccgtatta ccgcctggat cgcgccctgc
     6301 gcgatcatcc ccagctcgag acgctggggc gggtgacgct cggacagagc cttcgctcgg
     6361 taaacctcgt cctgtgggat gacaccagaa agcggctcat ctccttccgc gaagccgcgc
     6421 ttgcccgccg cagccgggaa tgcgccgcgc gcgctggtgg aatggtgcga cgggatgcgt
     6481 gtccggttta agggtcgcgt ctcgcactca caactcgtcc tgcagcgggg attgtttaag
     6541 ccgtcccttc attcctccct ttccattgcc gaaaagactc aaaaaataat tgtcatggcc
     6601 cggcgaagcg acagctgaga ccgggccacc cagaaacagg cgcacgtcat tctcgactgg
     6661 gtggcccgct cggagtttac actccggccg cgctctcgcg cggaccgggg tggcgggcca
     6721 tgacaatatt ttttttggtc tcacccatgc acacgcagga attcgttcgt ttccctaagg
     6781 cgcgttgcgg ccaaacgccc ttcgcgcctc gcaagggagc aatttgttga actggatctt
     6841 ctgggcggcg ccgttgctgg ttgcgatctc gggactcgcg ctgctgagct ccggaatcgg
     6901 acatttgctg cgcggccgcg cggtgcgggc gagcgggcat ctggttttcg gcgcgccgct
     6961 ggcggtcatc ggcttcgtgg cggggctgct cggcctcaac gcccagacct atgcgcggct
     7021 tacccatgag gggcccgtcg cgcaggtaag cgtgcgccgt ctcgatcccg catccgcacg
     7081 atggctcgtg accatccgcc gcctcgacgg cacgaaccgc attcaaacct gcgagctgca
     7141 gggagacgaa tgggtgctga gcggccgcgt gcagaaatgg aagccctggg ccaatctgct
     7201 cgggctcgat gcgacctaca cgctggagca gctgtcgaac atgtatttca ccgcccagcg
     7261 cggcaacggc aggccgatca ccgcctgcga catcgccgat gtggcgccca ggatcgatcg
     7321 atatctgccg ccttcatggc tcgcctggct cgcggcccgg agcttcgcgg tcgaccgccg
     7381 cttcggctcc gccaactata tgccgctggc cgacgccgcg gtctaccggg tcgtgatcac
     7441 gcaatccgga ttcaacgccg agccggtcaa ccccgccgcg cacgccgcga acgaggcgcg
     7501 gccatagtca tccggtagca gagtggagcg ttacgcaaga aagatttgac gcggaggtca
     7561 atgtttcgaa aacgt
//
LOCUS       DASZMC010000583         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2442016,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000583 DASZMC010000000
VERSION     DASZMC010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442016"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..618)
                     /locus_tag="VG274_11865"
     CDS             complement(163..618)
                     /locus_tag="VG274_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07400.1"
                     /translation="MRAADLANVQSVYILPMSHGMDQYLANRLTNGHVFRVVTDAKIA
                     DAVLTDHLGDTFQQELETLLPAPEAVKKVEAPKDKPAGPMLVDPDAKLPPVRSTFGGG
                     KGTLFLVDPKSHEVLWSVYDPAKGSDSKEMDRTASDIVSRLKKDLNPKK"
     gene            738..1412
                     /locus_tag="VG274_11870"
     CDS             738..1412
                     /locus_tag="VG274_11870"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWE07401.1"
                     /translation="MYKGQSIAVIIPCLNEEQGVEKVLRALPDFVDEVVVVDNASTDR
                     TCEVAAALGAKVIREDVRGYGRSYKRGFAVATSDVIITLDGDHSYPADALSYLIEAFL
                     HLDVDFLNASRFPVRDRRAMSFKNKFGNLVLSLAFSALFFRWVRDSQSGMWVFRRAIL
                     KEMKLESDGMAFSEEIKIEAVRNRKIRFGEISIQYSSRLGETKLNAWRDGFRNLSFLA
                     HKRFFR"
     gene            1409..2401
                     /locus_tag="VG274_11875"
     CDS             1409..2401
                     /locus_tag="VG274_11875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWE07402.1"
                     /translation="MTIAAVAPVWNGREWLRRMLASLARQTQPVAQIIAVDNGSTDGA
                     PDTAREFGARVISMGRNAGFAAAVNRGVQEACRIQPKAAWIAVLNTDVELADDYLARL
                     AACNSWFATGKLLKPGTTQIDGTFDLMSRSGAAWRAGSGREDGALFSERRAISFAPFT
                     AALFRAELFDRVGLLEERFESYLEDVDFGLRCAALGLEGIYEPMAVAWHKGSATLGRW
                     HPDTVRRIARNQIYLAARHFPSALLRQWFRPILAGRLLWGGVAARHGCTVAWARGVIE
                     GMRGFRSMRAASTPVEPERLRRLLSEHERLIREIQSATGFDAYWRWYFRLAGEA"
     gene            2408..>3120
                     /locus_tag="VG274_11880"
     CDS             2408..>3120
                     /locus_tag="VG274_11880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWE07403.1"
                     /translation="MASSQNDAIGIVIVTYNSAAEIGPCLDAAIASGAEVVVVDNGSR
                     DGTISQIARRGARLIANPENRGFAAAVNQGFRALSECAYVLLLNPDAVIESSLAPLRD
                     ACSRQKIAGAGGMLLGSDGAPQTGFMVRRFPTPASLILEAILLNRVWPGNPVNRRYRA
                     LDLCLDKESDVEQPAGAFLMTRRSVWEELQGFDEGFHPLWFEDVDYCKRAFDRGYMFR
                     FVPFAVARHTGGHSIPQLTV"
BASE COUNT          580 a         1002 c          873 g          665 t
ORIGIN      
        1 cttccacttt ccggcgatcg gtacatctat agctgccagc gccgcgccgg cggccagcgc
       61 gcacgccagt aagccgcgca aaataatcga tgaaaactgc atgctatgaa ttactcctga
      121 tgtccgcgat tatagcccgc atacatccgg agtagcgccg cgctacttct tcgggttcag
      181 atctttcttg aggcggctta caatgtcgga ggcggtgcga tccatttcct tcgagtccga
      241 accttttgca gggtcgtata ccgaccacaa cacctcgtgc gattttggat ctaccaggaa
      301 cagcgtgccc ttaccgcctc caaatgtgct gcgcaccggc ggcagctttg catccggatc
      361 caccagcatc ggtcccgcgg gcttgtcttt gggcgcctcg accttcttca ccgcttcggg
      421 agcgggcagc agcgtttcca actcctgctg gaaagtgtcg cccagatggt ccgtcagcac
      481 cgcgtcggca atcttcgcgt ccgtcaccac gcgaaacacg tgcccgttcg tcagccggtt
      541 agccagatac tggtccatgc cgtgcgacat gggcaaaata tatacggatt gcacgtttgc
      601 aaggtcggcc gcgcgcaagg caacaaaccc ggacgcaatc gctgcacaaa acaggagaag
      661 gcttacgcgc ttcatgggtt ccatcatatc aaccgacgct tttttcatta aaaaagtcga
      721 ttcggccgct attcgccatg tataaaggac agtccatcgc ggtcatcatt ccgtgtctga
      781 acgaagagca gggcgtggaa aaggtcctgc gcgcgctccc cgatttcgtg gacgaggtcg
      841 tcgtggtcga caatgcctcc accgaccgca cctgtgaagt tgcggccgcg ctcggagcca
      901 aagtcatccg tgaagacgtg cgcggctatg gccgcagcta caaacggggc ttcgcggtgg
      961 ccaccagcga cgtcattatc acgctcgatg gcgaccatag ctaccccgcc gatgcgcttt
     1021 cgtatctcat cgaagctttc ctgcacctgg atgtcgattt cctgaacgcc tcgcgcttcc
     1081 ccgtgcgcga ccgccgcgcc atgagcttca aaaacaagtt cggcaacctg gtgctctcgc
     1141 tggcgttttc tgcgctcttc ttccgctggg tgcgcgattc gcaatccggc atgtgggtct
     1201 tccgtcgcgc cattttgaaa gagatgaagc tcgaatcgga cggcatggcc ttctccgaag
     1261 agatcaaaat cgaagccgtg cgcaaccgca agattcgttt cggggagatc tccattcagt
     1321 attcgtcgcg cctcggtgaa accaaattga acgcctggcg ggacggcttt cgcaatctct
     1381 ccttccttgc ccacaagcga ttttttcgat gaccatcgcc gccgtggcgc cggtatggaa
     1441 cggccgcgag tggctgcgcc gcatgctggc tagccttgcg cgtcaaaccc agccggtggc
     1501 gcaaatcatc gcggtcgata acggttccac cgacggcgcg cccgatacgg cccgcgaatt
     1561 cggcgcgcgc gtcatctcca tgggccgcaa cgcgggcttc gccgctgccg tcaatcgcgg
     1621 cgtacaggaa gcctgccgca ttcaacccaa agccgcgtgg atcgccgtct tgaataccga
     1681 tgtcgaactt gcagacgact acctcgcccg tctcgccgcc tgcaattcct ggttcgccac
     1741 cggcaaactg ctcaagccgg gcactactca aatcgacggg accttcgatc tgatgagccg
     1801 cagcggggcc gcctggcgcg ccgggtcggg ccgtgaagat ggcgcgctgt tttccgaacg
     1861 ccgcgccatc tccttcgcac cgttcacggc cgcactgttt cgcgccgagc tcttcgaccg
     1921 cgtcggtctg ctcgaagagc gcttcgagtc gtacctcgaa gatgtcgatt tcggcttgcg
     1981 ctgcgcggcc ctcggtctcg aaggcattta cgaacctatg gccgttgcct ggcacaaagg
     2041 cagcgccact ctgggccgct ggcatcccga cacggtccgc cggattgcgc gcaaccagat
     2101 ttacctcgcg gctcgtcact ttcccagcgc tctgctgcgc cagtggttcc gtccgattct
     2161 ggctgggcgc ctgctgtggg gaggcgtggc tgcgcgtcac ggttgcaccg tcgcttgggc
     2221 tcgtggcgtc atcgaaggaa tgcgcggctt ccgttccatg cgtgccgcct ccactcccgt
     2281 cgaaccggaa agattgcgca gacttctttc tgaacacgaa cgtctaattc gcgagatcca
     2341 aagcgcaacc ggcttcgacg cttactggcg atggtatttt cgtctggcgg gcgaggcata
     2401 acgacacatg gccagtagcc agaacgacgc aatcggcatc gtcatcgtga cctacaattc
     2461 cgccgcagaa atcggcccat gcctggatgc ggccatcgca agcggcgccg aagtggtcgt
     2521 cgtcgacaac ggctcgcgtg acggcaccat ctcccaaatc gcgcggcgcg gcgctcgtct
     2581 cattgccaat cccgaaaatc gtggctttgc agcggccgtc aatcaaggct ttcgcgcatt
     2641 aagcgaatgt gcctacgtct tgctgctgaa tcccgacgca gttatcgaat cgagcctcgc
     2701 cccgcttcgt gatgcatgct cgcgccaaaa aattgcgggc gccgggggaa tgctgctcgg
     2761 ttcagacgga gccccgcaga ccggtttcat ggtgcgccgc tttcccactc cagcgtctct
     2821 gattttggaa gctatcttgc ttaatcgcgt ctggcccggc aatcccgtga atcgacgcta
     2881 ccgtgcgctc gacctgtgtc tcgacaaaga aagcgacgta gaacaaccgg ccggcgcatt
     2941 tcttatgact cgccgttccg tatgggaaga gttgcagggc ttcgacgaag gctttcatcc
     3001 tctctggttc gaagatgttg actattgcaa gcgcgcattt gaccgcggct atatgttccg
     3061 tttcgtccct tttgccgtcg cgcgacacac cggggggcac tctatcccac agctaacagt
//
LOCUS       DASZMC010000584         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3272134,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000584 DASZMC010000000
VERSION     DASZMC010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272134"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..408)
                     /locus_tag="VG274_11885"
     CDS             complement(145..408)
                     /locus_tag="VG274_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07404.1"
                     /translation="MPPMLVRLHVAKKQTGRSFKLWLPLFLLWLLALPVLIVTLPVVA
                     IVLVVLGRNPLRIFAAYWSLLSAIPGSHLEIESGRGLVFLHVY"
     gene            complement(408..773)
                     /locus_tag="VG274_11890"
     CDS             complement(408..773)
                     /locus_tag="VG274_11890"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HWE07405.1"
                     /translation="MADWQELMELTQGRAVVVERVRLADKDIAVEGAFELPQLARLDA
                     ADQVFVAAFVRSHGSIKEMERVFGVSYPTIKARLNRIAARLEFVDTNPGPTRADVLER
                     LKQGEISADDAIRELEGMH"
     gene            945..1106
                     /locus_tag="VG274_11895"
     CDS             945..1106
                     /locus_tag="VG274_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07406.1"
                     /translation="MEIFVPFLQMLERYNGMTMTPAPEKPVEAPAEDPFAGAFEIVAR
                     PKLTLVANR"
     gene            1179..1361
                     /locus_tag="VG274_11900"
     CDS             1179..1361
                     /locus_tag="VG274_11900"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HWE07407.1"
                     /translation="MIGIILLGALVLSPVVAYLGITPLPGDVTLNWEGHRIFLPFTQS
                     LIASVVLALLFFFARK"
     gene            1397..>2077
                     /gene="purH"
                     /locus_tag="VG274_11905"
     CDS             1397..>2077
                     /gene="purH"
                     /locus_tag="VG274_11905"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWE07408.1"
                     /translation="MTLRPIRRALLSVSDKTGLIDFAKGLAKHGTALLSTGGSAKALR
                     DAGLTVIDVSEVTQSPEMMDGRVKTLHPKIHGGLLALRDHKDHAEAMKTHGIEGIDLL
                     VSNLYPFEATVAKGAGFEETIENIDIGGPAMTRSAAKNHEWVTVIVDVEDYAAVLAEM
                     DANKGATTLALRRKLAQRAFARTGAYDAAVSNWFAGQLENEGEKDPPRRRVLGGHLRQ
                     ALRYGENPH"
BASE COUNT          432 a          664 c          590 g          391 t
ORIGIN      
        1 tatttcggca tgcctttcgg cgcaggcgac gacgcgggtg cgccatccag cgccgccatc
       61 aagcgctccg cctcgtcggc tgtgatgcgg ccggaggaga gcatttccaa gatttgtttg
      121 cgatcttcat tcatcgtctc aacctcaata gacatggagg aaaacaaggc cgcgcccgct
      181 ctcgatttcg agatgcgagc ccggaattgc gctcaacagg ctccaatacg ccgcaaaaat
      241 acgcagcgga ttgcggccca gcacaacaag aacgatcgcc acaaccggca acgtcacgat
      301 cagaaccggc aacgctaaca gccacagcag aaacagtggc agccagagtt tgaaacttcg
      361 cccggtttgc ttcttggcga cgtgcagtct caccagcatc ggcggcatca gtgcatcccc
      421 tccagttcgc ggatcgcgtc atccgcactg atctcgccct gtttcagacg ttcgagaaca
      481 tcggcgcgtg tcgggcccgg attggtgtcg acaaattcga gccgcgcagc gatgcggttc
      541 agccgcgcct tgatcgtggg ataggaaaca ccgaacacac gctccatctc cttgatcgag
      601 ccatggctgc gcacgaacgc cgcgacgaag acctggtcgg cggcgtcgag ccgcgcgagt
      661 tgcggaagct cgaacgcacc ctcgaccgcg atgtccttgt ctgcgagccg cacccgttcc
      721 acgaccacgg cgcggccttg ggtcaattcc atcaattcct gccaatcggc catgaaaacc
      781 cgccaaacgc ctcggttcca tatgagatag ataaactatc ttaattttca agtgccataa
      841 attgattttc ttaataaaaa tggaaaatta tatatcaatc aatcacttca gctttgatct
      901 aaacgtgacc cgaagtttta agccttggcg cgcaaactcc cgccatggag atcttcgtcc
      961 cctttctgca gatgcttgag cgctacaacg gcatgaccat gacgccggcg ccggaaaagc
     1021 ccgtggaagc gccggccgaa gacccatttg ccggcgcatt cgagatcgtc gcccggccga
     1081 agttgacact cgtcgcgaac cgctaaagac atgtgaccgg cgcgcgcacc gggcgccatg
     1141 cgccttgggg gctcgatttg cgttccggaa cggccattat gatcggcatc atcctgctgg
     1201 gcgcgctggt gctcagcccg gtggtcgcct atctcggcat cacgccgctg cccggcgacg
     1261 tcacgctcaa ctgggagggt caccgcatct tcctgccctt cacccagtcg ctgatcgcca
     1321 gcgtcgttct ggcacttctc ttttttttcg cgaggaagta acacccggcg taacccacgc
     1381 acgcccgagt ctcgccatga cgctccgccc catccgccgc gcgctgcttt ccgtttccga
     1441 caagacaggc ctgatcgact tcgccaaggg tctggcgaaa cacggcacgg cgctgctttc
     1501 gacgggcggt tcggcaaagg cgctgcgcga cgcaggcctc accgtcatcg acgtttccga
     1561 agtcacccaa tcgccggaga tgatggatgg ccgtgtgaag acgttgcatc cgaaaatcca
     1621 cggcggattg ctcgcgctgc gcgatcacaa ggatcacgcc gaagcgatga agacgcacgg
     1681 catcgagggc atcgatctgc tcgtctccaa tctctatccg ttcgaagcga ccgtggcgaa
     1741 gggcgccggc ttcgaggaga cgatcgagaa catcgacatc ggcggcccgg cgatgacgcg
     1801 ctcggccgcg aagaaccatg agtgggtcac agtcatcgtc gacgttgagg actacgcggc
     1861 cgttcttgcc gagatggatg cgaacaaggg cgcgaccacg cttgcgctgc gtcgcaagct
     1921 tgcgcagcgg gcgtttgcgc gcaccggcgc gtatgacgcc gcagtttcca actggtttgc
     1981 aggccagctc gaaaacgaag gcgagaaaga tccgccgcgc cgccgcgtat tgggcggaca
     2041 tctgcgccag gcgttgcgct atggcgagaa cccgcac
//
LOCUS       DASZMC010000585         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4639546,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000585 DASZMC010000000
VERSION     DASZMC010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4639546"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VG274_11910"
     CDS             complement(<1..1086)
                     /locus_tag="VG274_11910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWE07409.1"
                     /translation="MILSASRKLGKYEIMRKLGRGGMADVYLARDSESNRSVALKLIE
                     HVDDSGTRDIIDAERRGVSLQQQLSAIDSHVVRVYEFGDLEGFFFVAMEYVEGQDLAE
                     LMRGGPLAIEFATDAAIAVAETLDHAHNLRTVSEGRNYQGIVHGDIKPKNIRVDVRGD
                     IRVLDFGIAKALSLSRRLTRNEFGSVPYASPERLESGEVNAQSDIWSLAVMLYEMVTH
                     LQPYQAESTERLERMIRSRIPPPPAPDPCPEPLRRILMKALANDPAVRYASARDFANE
                     LILFRTGALVEPAGEEDFDRDATRRTTRPVSAPLANDETRRTTPIADRPGAAKLPAKK
                     ILVKLPVFSVFTRVVAILAVLCVLYGAW"
     gene            complement(1083..>1545)
                     /locus_tag="VG274_11915"
     CDS             complement(1083..>1545)
                     /locus_tag="VG274_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07410.1"
                     /translation="PSPPPPAPSYAAPPQPRPAFTYTPPSPPAYAYTALPQPRPVPVP
                     PPIARTGPRKFVIFAIAIATAILAGNLGLPIFSITAIVLGIFLGKTARSGFQRVAAVM
                     LIIFGTLALFARLGADAAALVGIAPLLFIGLGLFLLIRRLRPRPNGNWGRR"
BASE COUNT          298 a          378 c          620 g          249 t
ORIGIN      
        1 ccacgcgccg tagagtacgc agagcacggc caggatggcg acgacgcgag tgaagacgga
       61 gaagaccggc agcttgacca ggatcttttt ggcgggaagt ttagcggcgc cggggcgatc
      121 ggcaatgggc gtagtgcggc gggtttcgtc gttggcgaga ggcgcggaga cgggacgggt
      181 ggtacggcgt gtggcatcgc ggtcgaaatc ctcttcgccg gcgggttcga cgagggcgcc
      241 ggtgcggaag agaatgagct cgttggcgaa atcgcgcgcg gaggcgtagc ggacggctgg
      301 atcgttggcg agggccttca tcagaatacg gcggaggggc tccgggcaag ggtcgggcgc
      361 agggggcgga gggatgcggc tgcggatcat gcgctccagg cgttcagtgc tttcggcctg
      421 gtagggctgc aggtgcgtga ccatctcata gagcatgacg gcgagcgacc agatgtcgga
      481 ctgggcgttg acttcgccgg attcgagacg ctcgggagaa gcgtagggca cgctgccaaa
      541 ctcgttgcgc gtgaggcgac gggagagcga gagggctttg gcgatgccga aatcgagcac
      601 gcgaatgtcg ccgcggacat cgacgcggat gtttttgggc ttgatatcgc cgtgtacgat
      661 gccctggtag tttcggcctt cggagacggt gcgcaggttg tgcgcgtgat cgagcgtttc
      721 ggcgaccgcg atggcggcgt cggtggcgaa ttcgatggcc aacggaccgc cgcgcatgag
      781 ctcggcgagg tcttgacctt cgacatattc catggcgacg aagaagaagc cttcgagatc
      841 gccgaattca tagacgcgaa cgacgtggga atcgatggca gagagctgct gctggaggga
      901 gacgccgcgg cgttcggcgt cgatgatgtc gcgagtgccg gagtcgtcga catgctcgat
      961 gagtttcagc gcgacgctgc gattggattc ggaatcgcgc gcgaggtaga catcggccat
     1021 accgccgcgg ccgagtttac gcatgatttc gtatttgccc agcttgcggc tggcggagag
     1081 gatcaccggc gcccccaatt tccgttgggg cggggacgca ggcggcggat caacaggaag
     1141 agaccgagac cgatgaacag caacggggca atgccgacga gggcggctgc atcggcgccg
     1201 aggcgcgcga acagagcgag ggtaccgaag atgatgagca taaccgcggc gacgcgctgg
     1261 aaaccactgc gggcggtttt gccgaggaaa atgccgagga cgatggcggt gatggaaaag
     1321 atgggcaagc caagatttcc ggcgagaatt gcagttgcga tggcgatcgc aaagatgacg
     1381 aatttgcgcg gcccggtgcg cgcgatgggt ggaggcacgg gcacgggacg aggctgcggg
     1441 agcgcagtgt atgcgtatgc cggcggggac ggcggcgtgt aggtgaatgc aggacgaggc
     1501 tgcgggggcg cggcgtatga gggggcagga ggcggcggag aggga
//
LOCUS       DASZMC010000586         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2716234,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000586 DASZMC010000000
VERSION     DASZMC010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2716234"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..592)
                     /locus_tag="VG274_11920"
     CDS             complement(158..592)
                     /locus_tag="VG274_11920"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HWE07411.1"
                     /translation="MSALPFPRSAFDRVGGIVYFARMLDKIRLLAAGHLRTDYHANLG
                     AGLDGRCCRFLGVEYRSLCSRVQLGGSDEEIFEWCCQNGVRPNEEQVLVWNKFMLKRG
                     WRDEDDGSTQELEAYKAGSGLGHRADLPTFFEYYEVDEGRRP"
     gene            complement(589..1056)
                     /locus_tag="VG274_11925"
     CDS             complement(589..1056)
                     /locus_tag="VG274_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWE07412.1"
                     /translation="MHEYKATIQWKNAGPDFLKGRYSREHSWTFDGGLTVAASSAPGI
                     VPVPWSNPANVDPEEAFVASISSCHMLTFLWFAAKDGFQVVSYRDEAIGVLAKNERGA
                     MWVSDVTLHPNIAWMGAKTPTPEDLDRLHHRAHEECFIANSVKTTIRVAAPQT"
     gene            complement(1056..>2018)
                     /locus_tag="VG274_11930"
     CDS             complement(1056..>2018)
                     /locus_tag="VG274_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE07413.1"
                     /translation="LGVECELVYPGAPEVKHPTIEGFLVEKLKGPAPKTGARHPYHPP
                     EGVEVEEDVVYGTVDGTDLHVDIAHPKQVPDKPMPAVLLIHGGGWAGGTHKGYLPMQL
                     AEHGYFIATVEYRLSGEAPWPAQIEDCKLAIRWLRANAERYHVDPNRIGVMGHSAGGH
                     LVSCLGTLGNETSLDVGDFQGISSRVQAVVDEAGPVDFTPAGRPTLGTVTDDHPGLVK
                     LFGGSFDAKKDAWTQASAALHVSKDTPPFLILHGEKDTLVPIHQAEEMADALKKAGVP
                     VELIRVKNGNHGLHTDKPGDPPPDPNPEAQQELIRKFFDQQFNK"
BASE COUNT          351 a          603 c          665 g          399 t
ORIGIN      
        1 aaacggagat ctccaaaacc aacttcagaa tgccgccgga gagatcagcc ggagcctgga
       61 acaagcgtgg caggccgaag cggaggtcgc cctgaaagcg attcccgcgc cgccggccca
      121 gactgcccct gcgggaaagc agccttgatg gcggggctca ggggcgtcgc ccttcgtcca
      181 cttcgtagta ttcgaagaag gtcgggagat ctgcgcgatg gcccaggccg ctgccggcct
      241 tgtaggcttc gagttcctgc gtggagccgt cgtcctcatc gcgccagccg cgcttgagca
      301 tgaatttgtt ccagacgagc acctgttcct cattcgggcg cacgccattc tggcagcacc
      361 attcgaagat ttcttcgtcg ctaccgccca actgtacgcg actgcacagg gagcgatact
      421 ccacgccgag aaagcggcag caacggccgt cgaggccagc gccgagattg gcgtgataat
      481 ccgtgcgcaa atggcccgcg gctaacaagc ggatcttgtc gagcatccgc gcgaaataga
      541 cgatgccgcc gacgcggtcg aaggcgctgc gaggaaaggg cagggcactc atgtttgcgg
      601 cgccgccacg cggatggtgg tctttaccga attggcgatg aagcactctt catgggcgcg
      661 gtgatgcagc cggtcgaggt cctcgggcgt cggcgttttt gcgcccatcc aggcaatgtt
      721 tggatgcaac gtgacgtcgc tgacccacat ggcgccccgt tcgtttttgg ccagcacgcc
      781 gatggcttcg tcgcgatagc tcacgacctg gaagccgtcc ttggctgcga accagaggaa
      841 ggtgagcatg tggcaactgg agatggacgc gacgaaggct tcctcgggat cgacgtttgc
      901 gggattcgac catggaacag gaacgatgcc gggcgcggag gaagcggcca cggtgaggcc
      961 gccatcgaac gtccagctgt gctctcgcga atagcggccc ttgaggaaat ccgggccggc
     1021 atttttccac tgaatggtcg ctttgtattc gtgcattact tgttgaattg ctggtcgaag
     1081 aatttgcgga tgagttcctg ctgcgcttcc gggttcggat cgggaggcgg atcgccgggc
     1141 ttgtccgtgt gcaagccgtg gttgccattc ttcacgcgga tcaactcgac gggtacgccc
     1201 gctttcttca gcgcgtcggc catttcctcg gcctgatgta tcgggacgag cgtgtccttt
     1261 tcgccgtgga gaatgaggaa aggcggggtg tccttactga cgtgcagggc cgcgctggcc
     1321 tgcgtccacg cgtctttttt cgcatcgaag ctgccgccga aaagtttgac caagcccgga
     1381 tggtcgtccg tgacggtgcc gagagttggg cgacccgcag gcgtgaagtc gacggggccg
     1441 gcttcgtcga ccacggcttg gacgcggctg ctgatgccct ggaaatcgcc aacatcgagg
     1501 ctggtctcat tgcccagcgt tcccaggcac gagacgagat ggccgccggc gctgtgcccc
     1561 atgacgccga tacgattcgg gtcgacgtga tatctctccg cgttcgcgcg cagccagcgg
     1621 atggcgagct tgcaatcttc gatctgcgcg ggccagggcg cttcgccgct gaggcgatac
     1681 tccacggtgg cgatgaagta accatgctcc gcgagctgca tgggcagata gcccttgtgc
     1741 gtgccgcctg cccagccgcc gccgtggatg agcaggaccg cgggcatggg tttgtccggc
     1801 acttgtttcg gatgcgcgat gtcgacgtgc aagtcggtgc catccacggt gccgtagacc
     1861 acgtcttcct ccacttcgac gccctccggc gggtgatacg gatgccgcgc gccggtcttg
     1921 ggtgcagggc ctttgagttt ctcgacgagg aaaccctcga tagtcgggtg cttcacctct
     1981 ggcgcgccgg gatagacgag ttcgcattcc acgccgag
//
LOCUS       DASZMC010000587         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3539145,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000587 DASZMC010000000
VERSION     DASZMC010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3539145"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1573)
                     /locus_tag="VG274_11935"
     CDS             complement(173..1573)
                     /locus_tag="VG274_11935"
                     /inference="COORDINATES: protein motif:HMM:NF010693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HWE07414.1"
                     /translation="MSALWTSQEAEAATLGHATRTFEVQGVSIDTRTLKSGDLFVALR
                     GEARDGHEFVSRAIANGAAAGLVSRMPDDIAREAPLLVVANTQRALEDLSRAARIRAH
                     GRVVAVTGSAGKTTTKEMLRLALGALGSTHASASSYNNHWGVPLSLAQWPRGDAFGVF
                     EIGMNHFGEIRTLASFARPHVALVTTIAPAHLEYFGSVEAIADAKSEVFEGLAPGGVA
                     VVPADNACSARLIARARQAGVGRILSFGDSDAADARLLAWSEGSDGMKIEAEIMGRRC
                     MFRIGALGAHIAHNAIGALLSVAALEGDVVDAAASLASFSALKGRGARFSLPLAGGSA
                     EIIDESYNANPGSMRAALALLGSAKPGGGGRRIAVLGDMLELGAESPSLHAALSGAVE
                     HAHVDLVFACGRDMRALFDALPSSRRGVWGATSSAIAPQIIRMLRPGDVALIKGSLGS
                     RMSTILDALKNGAGAN"
     gene            complement(1570..>2747)
                     /locus_tag="VG274_11940"
     CDS             complement(1570..>2747)
                     /locus_tag="VG274_11940"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWE07415.1"
                     /translation="EHFGAQPRVVAAVTGTNGKTSVCVFLRQLWASRGRSAASIGTIG
                     VVAPSGSTPLAHTTPDAIETHRLLARLACEGVTHLALEASSHGLDQYRLDGVEIAAAA
                     FTNLTRDHLDYHPDFAHYLAAKLRLFTGLVRSGGLAVVNADAEEAGQFLSAARARNLR
                     LVTVGARGTQIRIASLAPCAEGQVLRIVFAERSYEIELPLVGAFQASNALIAAGIAIG
                     LGDPPEEIFAALSNLKGASGRLEKVAAATNGAPIYVDYAHTPDALETVLSALRPHVAG
                     RLHLVFGCGGDRDKGKRTLMGAIAARLADRAIVTDDNPRSEDAAEIRRQILVGCPGTT
                     EIGDRADAIRAAVAALLPGDVLVIAGKGHESGQIVGEETRPFVDSDEAKKAVREVAS"
BASE COUNT          514 a          922 c          874 g          437 t
ORIGIN      
        1 ggctgaccct tgccctgact cgcgcgcaag ctgcggatca tcgccggccc gatcacgaag
       61 gcgatgaaga gcgccgtcat gagcgcgccg ccagtgcgaa aagtgatgta gcggaagacg
      121 ttaagggcgc cgaggtgatc ggaaaaacgc acgagaagaa gcggaagcat ggctaatttg
      181 ctcccgcgcc gttcttgagt gcgtcgagga tcgtggacat gcggcttccc agggatccct
      241 tgatcagcgc cacgtctccc gggcgaagca tgcggatgat ctggggcgcg atggcggaag
      301 atgtcgcgcc ccacacaccg cggcgagatg acggcagagc atcgaacagc gcgcgcatgt
      361 cgcggccgca ggcaaagacg aggtcgacgt gtgcatgttc gacggcgccg gaaagcgccg
      421 cgtgcagcga agggctttcg gcgcccagct ccagcatatc tcccaacacg gcgatgcgcc
      481 tgccgccgcc gcccggtttt gccgaaccca gaagcgcgag cgcggcgcgc atcgacccag
      541 gattggcgtt gtagctctcg tcgatgattt cggccgatcc gcccgcgagc ggcagagaga
      601 accgcgctcc gcgacccttg agggcagaaa aggatgcaag cgacgccgcc gcatcgacga
      661 catcgccctc aagtgcagcg accgacagca gggcgccgat tgcattatgc gcgatatggg
      721 cgcccaacgc tccgatccgg aacatgcatc gccggcccat gatctccgct tcgatcttca
      781 tgccatcgga gccttccgac caggcgagga gccgggcatc cgcggcgtcc gaatcgccga
      841 agctcagtat gcggcccacg cccgcctggc gcgcgcgggc gatcaggcgg gcagagcagg
      901 cattgtccgc cggcacgacg gcgacgccac ctggcgccaa gccctcgaaa acctccgact
      961 tggcgtcggc gatcgcttca acgctgccga aatattcgag atgtgccgga gcaatcgtgg
     1021 tgacgagcgc gacgtgcggc cgcgcgaagc tcgcaagcgt ccggatctcg ccgaaatgat
     1081 tcatgccgat ctcgaatacg ccaaaggcgt cgccgcgagg ccattgcgcc aggctcagcg
     1141 gcacacccca atgattgttg taggacgagg ccgacgcatg ggtggaacca agcgcgccga
     1201 gcgcaaggcg cagcatctct ttcgtcgtgg tcttgccggc gctgccggta accgccacca
     1261 cgcggccatg ggcgcggatt cgcgccgcgc gactgagatc ctcgagcgcc cgctgggtgt
     1321 tggcgacgac aagcagcggc gcttcgcgcg ctatatcgtc cggcatacgc gacaccagcc
     1381 ccgcagcggc gccgttcgcg atcgcccgcg acacgaattc atgcccatcg cgcgcttcgc
     1441 cccggagcgc cacgaacaga tcgccggatt tcagcgtgcg cgtgtcgatc gacacgccct
     1501 gcacctcgaa ggtgcgcgtg gcgtgaccga gagtcgccgc ctccgcttcc tgcgatgtcc
     1561 agagtgcgct cacgacgcaa cctcgcgcac cgcttttttt gcctcgtcgg aatcgacgaa
     1621 gggacgggtc tcttcgccga caatctgtcc actctcatga cctttgccag cgatgacgag
     1681 aacatcgccc ggcaaaagtg cggcaacggc cgcgcgaatc gcatcggccc tatcgccgat
     1741 ttccgtcgtg ccgggacaac ccacgagtat ctggcggcga atttccgccg catcctcgct
     1801 gcgtggattg tcatcggtca cgatggcacg gtcggcaagc cgcgcggcga tcgcccccat
     1861 caatgttctt ttgcccttgt cgcggtcacc gccgcagccg aaaacgagat gcagcctgcc
     1921 ggcgacatgc ggccgaagtg cggaaagcac ggtttcgagc gcgtccggcg tgtgcgcgta
     1981 atcgacatag atcggcgcgc cgttcgtcgc cgccgccact ttctcaagcc gaccagaggc
     2041 gcccttcaga tttgacagcg cggcgaaaat ttcttccggc ggatcgccca atccgatcgc
     2101 aattcccgcg gcgatgagcg cattggatgc ctgaaaggcg cccaccagcg gcaattcgat
     2161 ctcataactt ctttccgcaa agacgatccg caggacctgc ccttcggcgc acggcgcaag
     2221 agaagcaatt cgaatctgcg tccctcttgc gccgacggtg acgagcctga gatttcgcgc
     2281 gcgcgccgcc gacagaaact gcccggcctc ctccgcatcc gcattgacga ccgccaatcc
     2341 gccggaccgg acaagcccgg tgaacaggcg caacttcgcc gcgaggtaat gcgcaaaatc
     2401 gggatgatag tccaaatggt cgcgtgtgag attggtgaag gccgctgctg cgatttccac
     2461 gccgtcaagg cggtactggt cgagtccgtg gcttgacgcc tcgagcgcga gatgggtgac
     2521 gccctcgcac gcaaggcgcg cgagcagcct gtgcgtctcg atggcatcgg gagtggtgtg
     2581 cgcaagcggc gtgctgcccg acggcgcgac aaccccgatc gtgccgatgc tcgcggcgct
     2641 gcgaccgcgg ctcgcccaca gctgacgcag gaaaacgcag accgatgtct tcccatttgt
     2701 gccggtgacc gccgccacga cgcgcggctg ggcgccgaaa tgttcgg
//
LOCUS       DASZMC010000588         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1063301,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000588 DASZMC010000000
VERSION     DASZMC010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063301"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..938
                     /locus_tag="VG274_11945"
     CDS             207..938
                     /locus_tag="VG274_11945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWE07416.1"
                     /translation="MRKMKATRKVAPRELLRIEGRAVEVNVRINPRARRLIVKVHPTT
                     GEVTVTAPSQRAFVKALEFARGQHDWIATQLSRVPVPVTLSTGSQVPFKGRDLVVREG
                     ESGHGTVWAEGDILYVTGGKTHQPRRILDFLKREARKEFEACTLEYGARLGVRHKRIT
                     VRDTASRWGSCSAQRSLSYSWRLILAPAFVLDYVVAHEVAHMREMNHGPQFWKLVRDL
                     VGDPRRAQNWLRDHGAALHRYAPKG"
     gene            complement(935..1393)
                     /locus_tag="VG274_11950"
     CDS             complement(935..1393)
                     /locus_tag="VG274_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07417.1"
                     /translation="MLGRLFPRQFDNVYRGHWLALILLGLLLALDAVIGTNSILNTRT
                     VMIGADGIPLDSFSPQAAQEAISEFALLGLWRLFFVLIGVVALIRYRAMVPFLFLLLI
                     VQQLGGRVVHALHPTDGGVTQAGSIVVWGMLGVIVLGFVLSLIGKSRPAD"
     gene            1544..1906
                     /locus_tag="VG274_11955"
     CDS             1544..1906
                     /locus_tag="VG274_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07418.1"
                     /translation="MRAWIAYAATGAVLLGSPAMANDGTGKGHSGSGPVALSAAQPDF
                     ESQQIGRVVAVDPAGMTLKCHWVAEEWTYHVTPKTTFRKNGARTSLADLKSDDVVQVL
                     FHREGTNQVADAVIVSTQ"
BASE COUNT          409 a          630 c          650 g          332 t
ORIGIN      
        1 gtcgttctgg cttaaatggg caacgatctg gtgaccgaag aaatcgaaat cgacccagtc
       61 gtcgctcgcg cggccttccg cgcagccaag gatgccggtg tagaacgcgc gcgctgatgc
      121 gaggtcgtga accggaaaag cgagatggaa gcgcggccgt gacgtcattt ccgtttcttc
      181 gatggtttgg tgtgcaaggg aagcccatgc gtaagatgaa ggcaacccgt aaagtcgcgc
      241 cgcgcgagct cctccgtatc gaggggcgcg cggtggaagt gaacgtgcgg ataaacccgc
      301 gcgcgcgacg gttgatcgtc aaggttcatc ccacgacggg cgaggtgacg gtcaccgcgc
      361 cgtcgcaacg cgccttcgtc aaggcgctcg aattcgcccg cggccagcac gactggatcg
      421 cgacgcaatt gtcgcgcgtg ccggtgccgg tgacgctgtc gacaggttca caggtgccgt
      481 tcaagggccg cgaccttgtc gtgcgcgaag gcgaaagcgg gcacggcacg gtctgggcag
      541 aaggcgatat tctctatgtc accggcggca agacacatca gccgcgacgc attctggact
      601 tcctgaaacg cgaagcgcgc aaggagttcg aggcgtgcac gctggaatat ggggcgcgtc
      661 tcggtgtccg gcacaagcgt atcacggtgc gcgatacggc gagccgctgg ggatcgtgct
      721 cggcgcaacg ctcgctgtca tactcctggc ggctgatcct cgcgccggcg ttcgtactcg
      781 attatgtcgt ggcgcacgaa gtcgcccata tgcgcgagat gaaccacggg ccacaattct
      841 ggaagctggt ccgcgatctc gtcggcgatc cgcgcagggc gcagaactgg ctccgcgatc
      901 acggcgcggc gctccatcgc tacgcgccga agggctaatc ggccggccgg gacttcccga
      961 tcagcgacag gacaaagccc aggacgatta caccgagcat cccccatacg acgatggagc
     1021 ccgcctgcgt gacgccgcca tccgtcgggt gcagggcatg caccacgcga ccgccgagtt
     1081 gctgcacgat caacagcaga aacaggaacg gcaccatcgc gcgatagcgg atcagcgcaa
     1141 cgacacctat cagcacaaag aacagccgcc acagtcccag cagcgcgaat tcggagatcg
     1201 cctcctgtgc ggcctgcggg gaaaaactgt cgagcgggat gccgtccgca ccgatcatca
     1261 ccgtgcgcgt gttgaggatg gaattcgtgc cgatcaccgc gtccagcgcc agcaaaaggc
     1321 caagcaggat caatgcaagc caatggccgc gatagacatt atcgaactgc ctcggaaaaa
     1381 gtcgtcccaa catgttcccg ccctcatgcc gcttacagat ttgatgtaag cgctgagggc
     1441 acatttccaa ggagtccgcc cgaccctgcg aacatttttg acccgttctg gcgcggcgcg
     1501 ggccgacggc ctaagctcgc gcgacgtcgc tagggagatt tcgatgcgcg catggatcgc
     1561 ctacgccgcg acaggtgctg ttctgcttgg gtcgcccgcc atggccaacg acggcaccgg
     1621 caaaggccat agcgggagcg gacctgtcgc gctgagcgcc gcccaacccg atttcgagtc
     1681 ccagcagatc gggcgtgtcg tcgccgtcga tccggcggga atgaccttga agtgccattg
     1741 ggttgctgaa gagtggacct atcacgtcac gccgaagacc acgttccgca agaacggcgc
     1801 gcgcacgagc ctcgccgatc tcaaatccga cgatgtggtc caggttctgt tccacaggga
     1861 agggacaaac caagtcgcgg acgcggtgat cgtttcgacc cagtaagggc gcctatccgg
     1921 cggttgcgag gtcgcgcgcg agcgtgtcgt gcgcggcctg ccagtcctgg aacagggggc
     1981 gcagcatcag cgctttccac ccgggctcat cgaaaaatgc c
//
LOCUS       DASZMC010000589         5437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2162693,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000589 DASZMC010000000
VERSION     DASZMC010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162693"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VG274_11960"
     CDS             <1..1062
                     /locus_tag="VG274_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWE07419.1"
                     /translation="MDGFESNEGVILVAATNRPDVLDPALLRPGRFDRRVVVGRPDVQ
                     GREAILRVHTKKVPLGDNVDLSVLARGTPGLSGADLANVVNEAALNAARQNRKVVMMI
                     DFEVAKDKILMGAERKSLVMSDAEKRNTAYHEAGHALVAALLPDADPLHKVTIIPRGM
                     ALGVTMQLPIDDKHSYDREYLQAQLAILMAGRIAEEKYMHHMTTGAGNDIERATDMAR
                     KMVCEWGMSDLGPLSFGKKEEQIFLGREIAQHRDYSEETAIRIDEQVKKLVQAGYDTA
                     ASVIEQHSEALAKIAETLLIREILDGNEVMQIIKGGDLPPQSTSGLKDTADHTQQVIR
                     PESGRRLPGFNEGERPQPA"
     gene            1062..1703
                     /locus_tag="VG274_11965"
     CDS             1062..1703
                     /locus_tag="VG274_11965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWE07420.1"
                     /translation="MSAGCVPRFGAYLFDIDGTLLDSAHDICGAIQQTLEPTGCGELP
                     FDYLRGFIGLHLIDCFADVLPHYTPEQMDELVQNYRGHYPARGHKQTSIFPGVAEALA
                     ALNAPKTTATTKGTHGSRAILEQFGLLPYFNHVQGTDGFPCKPAPDVLFRGIQALGAK
                     PEDCLMVGDAPSDMIAGKRAGVKTCAVRYGYGKPEELAALEPDYWISDLRELL"
     gene            1749..2396
                     /gene="alkB"
                     /locus_tag="VG274_11970"
     CDS             1749..2396
                     /gene="alkB"
                     /locus_tag="VG274_11970"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: protein motif:HMM:NF011930.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HWE07421.1"
                     /translation="MSSLFDDVPSPPSREPLEDGAVLLRGFASDAAPQLLEDVSRIAA
                     AAPFRHLMTPGGRIMSVGMTNCGRVGWVSDRKGYRYDPNDPDTGSPWPAIPESFRDLA
                     LRAASEGGFEAYDPDACLINRYVPGAKLSLHQDRDEKDAWAPIVSVSIGIPAVFIWGG
                     KHRGDPTRRMRLENGDVVVWGGPARFAYHGIAPLKEAEHPLTGVIRINLTFRKVF"
     gene            2451..3215
                     /gene="trpC"
                     /locus_tag="VG274_11975"
     CDS             2451..3215
                     /gene="trpC"
                     /locus_tag="VG274_11975"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWE07422.1"
                     /translation="MARIVATKREEVSAHSASLAEWESAAQLRLSQRRDFIQSLRSHT
                     PAIIAEVKKASPSRGVLSPDFDPPQIAAAYESGGAAAISVLTDAPYFQGSLADLEAAR
                     AAINLPVLRKDFTIATSQIVEAAAHSADAILLIAAVLSERELRDFREFATRYSLAALV
                     EVHNRPELDAAIASGATLIGVNNRDLATFEVTLETSLRLAEHIPSGALLVSESGIRDA
                     RDIATLRAAGYQAFLVGEHLMKSGDPAATVKELVSE"
     gene            3212..3823
                     /locus_tag="VG274_11980"
     CDS             3212..3823
                     /locus_tag="VG274_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWE07423.1"
                     /translation="MIVKVCGITRAADATAAVAAGANAIGFNFYPRSPRYIAPEQAAL
                     ILTPHARRVGVFVNESPERVAEIARLASLDTVQLHGDENPADYPAQFAIWKAIRVAAD
                     FDLNTLNADSAEAFLLDGPAGALYGGSGIAFDWNRARLARLRIIIAGGLDGDNVARAI
                     EASRPWGVDACSRIESSPGIKDHVKMQAFVQAALAAAPEALAL"
     gene            3820..5025
                     /gene="trpB"
                     /locus_tag="VG274_11985"
     CDS             3820..5025
                     /gene="trpB"
                     /locus_tag="VG274_11985"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWE07424.1"
                     /translation="MIGQVPQPDTSGHFGRYGGRFVPEVLMAPIEELEQAYLKARNDP
                     EFQSELAALLHNYAGRPTPLYFAKRLSESIGGARIWLKREDLLHTGAHKINNALGQAL
                     LAQRMGKRRIIAETGAGQHGVATATVCALLGLECVVYMGEEDMRRQRLNVFRMRLLGA
                     KVEGVTIGSRTLKDAISEAMRDWVTNVADTYYLLGSALGAHPYPMMVRDFHKCIGEEA
                     RRQILESDSRLPDSIFACVGGGSNAIGIFHAFIPDASVKLIGIEAGGRGHGIGEHAAR
                     FRGGAPGVLQGSYSYLLQDDNGQVSSTHSVSAGLDYPMIGPEHAWLHDQNRAEYVAVS
                     DAEALAAALQLSRTEGIIPALESSHALAEAFRRAPEAKGQTFIVNVSGRGDKDIDIYR
                     ENFPELDQA"
     gene            5022..>5437
                     /gene="trpA"
                     /locus_tag="VG274_11990"
     CDS             5022..>5437
                     /gene="trpA"
                     /locus_tag="VG274_11990"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWE07425.1"
                     /translation="MTRIGKLFETLKRDSRKGLIAYITAGDPSPAQTPALVEALVRGG
                     ADLIELGVPFSDPIADGPVIQRAGERALAAGTTLAGVLEIARQIRRQSEVPLLLFTYL
                     NPVVRYGLDRLAKDAAAVGIDGCLLIDASVEEAHDYV"
BASE COUNT         1032 a         1912 c         1479 g         1014 t
ORIGIN      
        1 atggacggtt tcgagtccaa cgagggcgta atcctcgttg ccgctaccaa ccgtcctgac
       61 gtgcttgatc ccgcgctgct gcgtcccggc cgttttgatc gtcgcgtagt ggtcggccgt
      121 cccgatgtcc agggccgcga agccatcctt cgtgttcaca ccaaaaaggt tccgctgggc
      181 gacaacgttg atctttccgt gcttgcgcgt ggaacaccgg gcctttcggg cgccgatctt
      241 gccaatgtgg ttaacgaagc tgcgctgaac gccgcgcgcc agaaccgcaa ggtcgtcatg
      301 atgatcgatt tcgaagtcgc caaggacaaa atcctgatgg gcgccgaacg caagtcgctg
      361 gtaatgagtg atgccgaaaa gcgcaacacc gcgtatcacg aagctggcca cgcgcttgtc
      421 gctgcgttgc ttcccgacgc cgatccgctc cacaaggtga ccatcatccc gcgcggtatg
      481 gccctgggcg tgaccatgca gttgccgatc gatgataagc attcctacga ccgagagtat
      541 ctgcaggccc aacttgccat tctgatggcc ggccgcatcg ccgaagagaa gtacatgcac
      601 cacatgacga cgggcgccgg caacgacatc gaacgcgcca cggacatggc tcgtaaaatg
      661 gtctgcgagt ggggcatgag cgacctcggc cccctcagct tcggcaagaa ggaagaacag
      721 atcttcctcg gccgcgaaat cgctcagcac cgcgactaca gcgaagagac cgcgattcgc
      781 attgatgagc aggttaagaa gctggttcaa gccggatacg acactgccgc cagcgtcatc
      841 gagcagcact ccgaggcact cgcgaagatc gccgagaccc ttttgatccg cgaaattctc
      901 gacggcaacg aagtcatgca gattatcaag ggcggcgacc tcccgcccca gtccacctcc
      961 ggcctcaagg acactgctga ccacacgcag caagtgatcc ggcccgagag tggccgccgt
     1021 ctgcccggtt tcaacgaggg cgagcgcccg cagccggcat aatgtcggcc ggttgcgtgc
     1081 cccgcttcgg ggcttacctg ttcgatattg acggcacgct gctcgattct gcccacgaca
     1141 tatgcggcgc cattcagcag acgctcgaac caacgggttg tggtgaattg ccgttcgact
     1201 atctgcgcgg cttcatcggt ctccatctga tcgattgttt tgcggatgtg ctgccccact
     1261 acacgccgga acagatggat gaactggtgc aaaattaccg cggccattat cccgcgcgcg
     1321 gtcacaagca aaccagcatc tttcccggcg tcgccgaggc gcttgccgcg ctgaacgcgc
     1381 ccaaaaccac tgctacaaca aagggcaccc acggatcgcg cgccatcctg gagcagtttg
     1441 gcctgctgcc ttatttcaat cacgtccaag gcaccgacgg tttcccgtgc aagcccgcgc
     1501 ccgacgtact cttccgcggc atccaggctc tcggagcgaa gcccgaagac tgcctcatgg
     1561 tcggcgacgc gcccagcgat atgatcgccg gcaagcgcgc cggagtcaaa acctgcgccg
     1621 tgcgctacgg ctacggaaaa cccgaagaac tcgccgctct cgagcccgat tactggatca
     1681 gcgacctccg cgagctcctc taatctctcc caccgcgccc atcgtcgcgc gatcatgaaa
     1741 gcaagcccat gtcgtcgctt ttcgatgatg ttccctcgcc gccttcccgc gaaccgctcg
     1801 aagacggcgc cgttttgttg cgtggcttcg catccgatgc agccccgcaa ctgctcgaag
     1861 atgtttcacg catcgccgcc gccgcccctt tccgccatct gatgaccccg ggcggccgca
     1921 tcatgtccgt tggcatgacg aattgcggcc gcgtcggctg ggtctccgac cgtaaaggct
     1981 atcggtatga tcctaacgat cccgatacag gaagtccctg gcccgccata cccgaatctt
     2041 ttcgcgatct cgcgctacgc gccgccagcg agggcggatt tgaagcctac gatcccgacg
     2101 cctgtctcat caaccgctac gtcccgggcg ccaaactcag cctgcatcag gaccgcgacg
     2161 aaaaagacgc ctgggcgccc atcgtttccg tctccatcgg cattcccgcc gttttcatct
     2221 ggggcggcaa acatcgtggc gaccccaccc gccgcatgcg cctcgaaaat ggcgacgtcg
     2281 ttgtctgggg cggccccgcg cgtttcgcat accatggcat cgcgccgctc aaagaagccg
     2341 agcacccgct caccggcgtc atccgcatca acctgacatt ccgcaaggtg ttctgattct
     2401 gtcgctacgt taaaataagg gaacccttga tccaaactgt tccagacata ttggctcgaa
     2461 ttgtcgccac caagcgagaa gaggtgtcgg cgcactcggc gtcgcttgcg gaatgggaat
     2521 ctgccgccca attgcgcctc tcccaacgcc gcgatttcat tcaatcgctg cgcagtcaca
     2581 cgcccgccat cattgctgaa gtaaaaaagg cgtcacccag ccgcggcgtt ctctcccccg
     2641 atttcgaccc tcctcaaatt gccgcagcct acgaatccgg tggcgccgcc gctatctccg
     2701 tgctcactga tgcgccatat ttccagggct ctctcgccga cctcgaagcc gcgcgcgccg
     2761 cgattaacct tcccgtgctg cgcaaagact tcaccatcgc cacgtctcaa atcgtcgaag
     2821 ccgccgcgca ctccgccgac gccatccttc tcatcgccgc cgtcctgtcg gagcgcgaac
     2881 tgcgcgactt tcgcgaattc gcaacgcgct actcgctggc cgcgctcgtt gaagtgcata
     2941 accgcccgga actggacgcc gccatcgcgt ccggcgccac tttgatcggc gtgaacaatc
     3001 gcgacctcgc cacattcgaa gtcacgctcg aaacctctct gcgtcttgcc gaacacatcc
     3061 cttccggagc tcttctcgtg agcgaaagcg gcatacgcga cgcgcgcgat atcgccactc
     3121 tgcgggccgc cggctatcag gcctttctgg tcggtgaaca cctgatgaaa tccggcgatc
     3181 ccgccgctac agtaaaagag ctggtctcgg aatgatcgtc aaggtctgtg gcatcacgcg
     3241 cgcggcggat gcaactgctg ctgtggcagc gggcgccaat gccataggct tcaacttcta
     3301 tccgcgcagt ccgcgctaca tcgcgccgga acaggccgcg ctcattctca ctccgcacgc
     3361 ccgccgcgtt ggcgtgttcg tcaacgaatc gcccgagcgt gtcgccgaaa tcgctcgcct
     3421 cgcgtcgcta gacaccgtac aactgcacgg cgacgagaat cccgcggact atcccgcgca
     3481 atttgccatt tggaaagcca tccgcgttgc ggcggacttc gatctcaaca ccctcaacgc
     3541 ggattccgct gaggcttttc tgctcgatgg tcccgcgggc gctctctacg gtggctccgg
     3601 catcgccttc gattggaatc gcgcccgtct cgcccgcctc cgcatcatca tcgcgggcgg
     3661 tctcgatggc gacaatgtcg cgcgcgccat tgaggcttca cgaccctggg gcgtcgacgc
     3721 ctgttcgcgc atcgaatcgt cccccggaat taaggatcac gtaaaaatgc aagcattcgt
     3781 ccaagccgct ctggcagccg cgcccgaggc gctcgcttta tgatcggcca agtcccccag
     3841 cccgacactt ccggccactt cggtcgctac ggcggtcgct tcgtccccga agttctcatg
     3901 gcgcccatcg aggaactcga gcaggcgtac ctcaaggcgc gcaacgatcc cgaatttcag
     3961 tccgagctcg ccgcgctgct gcataactac gccggccgcc cgacgcctct ctatttcgcc
     4021 aagcgtctca gtgagtccat cggcggcgcc cgcatctggc tcaaacgcga agacctcctc
     4081 cacaccggcg cgcacaagat caacaacgcg ctcggccagg cgttgctcgc ccaacgcatg
     4141 ggcaaacgac gcatcatcgc cgaaaccggc gcgggccagc acggcgtcgc caccgccacg
     4201 gtttgtgcgc tgctcggcct cgaatgcgtt gtctatatgg gcgaagagga catgcgccgc
     4261 cagcgcctga atgtctttcg catgcgtctg ctcggcgcca aagtcgaagg cgtcaccatc
     4321 ggcagccgca cgctcaaaga cgccatcagc gaagccatgc gcgactgggt gaccaacgtc
     4381 gccgacacat actatctgct cggctcggct ctcggcgcgc atccgtaccc gatgatggtg
     4441 cgcgattttc ataaatgcat cggcgaagag gcgcgccgcc agatcctcga atccgactct
     4501 cgcctgccgg actccatctt cgcctgcgtc ggcggcggtt ccaacgcaat cggaattttc
     4561 cacgccttca ttcccgacgc ttccgtgaaa ctcatcggca tcgaagccgg cggccggggc
     4621 cacggcatcg gcgaacacgc ggctcgattc cgcggcggcg ctcccggcgt cctgcaaggc
     4681 tcgtacagct atcttctaca ggacgataac ggtcaggtct cctccacaca ctccgtttcc
     4741 gctggcctcg attaccccat gatcggcccc gaacacgctt ggctgcacga tcaaaaccgc
     4801 gccgaatacg tcgcggtgtc ggacgccgaa gctctcgccg ccgccctgca gctctcgcgc
     4861 accgaaggca tcatccctgc gctcgaatcg tcgcatgcgc tggctgaagc cttccgccgc
     4921 gcgcccgaag ccaaggggca aaccttcatc gtcaacgtct ccggccgcgg cgataaagac
     4981 atcgacatct accgcgaaaa cttcccggaa cttgatcagg catgacgcgc atcggcaagc
     5041 ttttcgaaac cctcaaacgc gatagccgca agggcctcat cgcctacatc actgccggtg
     5101 acccttcgcc cgcgcagacc cccgcgctcg ttgaagccct cgttcggggc ggcgccgatc
     5161 tcatcgaact gggcgtaccg ttttctgacc caatcgccga tggccccgtc attcaacgcg
     5221 caggtgaacg cgctctcgca gccggaacca cgctcgccgg ggtgctcgaa atcgcgcgcc
     5281 agatccgccg ccagtccgaa gttcccctgc tcctgttcac gtatctcaat ccggtcgtcc
     5341 gctacggact cgatcgtctc gccaaagacg ccgccgcggt cggcatcgat ggctgcttgc
     5401 tcatcgacgc cagcgtcgaa gaggctcacg attacgt
//
LOCUS       DASZMC010000590         6300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4095954,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000590 DASZMC010000000
VERSION     DASZMC010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4095954"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VG274_11995"
     CDS             <1..155
                     /locus_tag="VG274_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008756960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWE07426.1"
                     /translation="SVRLRISMNALRSVDRSGGLDAFLMRAKDGLLSGRALRFKRLIL
                     KKQAAA"
     gene            complement(152..2011)
                     /gene="cobT"
                     /locus_tag="VG274_12000"
     CDS             complement(152..2011)
                     /gene="cobT"
                     /locus_tag="VG274_12000"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HWE07427.1"
                     /translation="MKPETPAEPFKRAVACAVRSLAGEPELEVNFSAEPSMLKGLKAR
                     LPLPSRNLPPSEIAVMRGQGDAYALRLAYHENKLHEMYRPKSADAAAIFEAAEQARVE
                     AIGARAMKGVAANLTAGLEARLGASSLARARERGEAPLAEIIGLIVRERITGLPPPEC
                     ARPAVDAWRSWIEERAGEDLARLEGVHRDQKAFARLTRAILKDFEMGDDLGEKSESEG
                     DESSETEPQSEDAGETADAAARPEPENAEQSESEGQDSDELADEIGAEQTAEPSGLEE
                     DNARTKPWRPEQPFSRQDEWGYRIHTTQFDEIVGATDLCDAEELTRLRNFLDQQLQAM
                     QGVVARLANKLQRLLLAQQNRSWEFDVEEGLLDTARLPRIIIDPMYSLTYKREKDMSF
                     RDTVVTLLLDNSGSMRGRPIMVAAMCADILARTLERCAVKVEILGFTTRAWKGGQSRE
                     KWIAEGKSPHPGRLNDLRHIIYKPADEPWRRTRRNLGLMMREGLLKENIDGEALIWAH
                     QRLMARLEQRRILMVISDGAPVDDSTLSVNAGTYLERHLRHVIEDIEEKSAIELVAIG
                     IGHDVTRYYRRAVTIVDAEQLGGAMTGQLISLFGEHEFHGRSAGRSRLRARAA"
     gene            complement(2008..3000)
                     /gene="cobS"
                     /locus_tag="VG274_12005"
     CDS             complement(2008..3000)
                     /gene="cobS"
                     /locus_tag="VG274_12005"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HWE07428.1"
                     /translation="MIPDTQGLDPTATPDTKVDVHAVFGIDAAMKVPAFSRGNEYVPD
                     RDSAYRFDRETTLAILAGFAFNRRVMIQGYHGTGKSTHIEQVASRLNWPCVRINLDSH
                     ISRIDLIGKDAIVLREGQQVTEFREGLLPWALQHPTALVFDEYDAGRPDVMFVIQRVL
                     EVQGRLTLLDQNRVIRPHPAFRLFSTTNTIGLGDTTGLYHGTQQINQGQMDRWNIVTT
                     LNYLTHDAEVEIVLAKVPSYGTAERKKLISAMVRVADMTRNAFVNGDLSTVMSPRTVI
                     TWAENAEIFNDVGFAFRLTFLNKCDELERASVAEFYQRSFNDELPESSAKVLVA"
     gene            complement(3069..3518)
                     /locus_tag="VG274_12010"
     CDS             complement(3069..3518)
                     /locus_tag="VG274_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWE07429.1"
                     /translation="MWFCLQHARQHNEHWDYFNGMSESEIEAFRVDAVTGHRPTWPLG
                     KRAAQVHRAQGFRSFRDAYGVFADDSGDPAAAEKRRAERPLTRPQALALDVLNLEAGA
                     SLHQIKARYKELVKRFHPDANGGDRGAEERLKRVIQAYGVLRASGLT"
     gene            3758..4042
                     /locus_tag="VG274_12015"
     CDS             3758..4042
                     /locus_tag="VG274_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HWE07430.1"
                     /translation="MSIADEIQRKLSQGLAPTELYIEDESAHHAGHSGARPEGETHFR
                     VRVVSTVFESMGRLARQRLIHELLSEELRVHVHALSIEARTPNEAGSTRS"
     gene            complement(4052..5350)
                     /locus_tag="VG274_12020"
     CDS             complement(4052..5350)
                     /locus_tag="VG274_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HWE07431.1"
                     /translation="MASFLVIGIGTIIVLLAVSAFLSGSETALTAVSPATMHRLEQRN
                     SRAARPVNMLLADRERMIGAILLGNTFINILASSLATSLLAAHYGNTAVIITALLMTF
                     VVLVFAEVLPKTLAIARTDRFALSVGRILLPVVNLLAPVVDSVQFVVWRVLRLFGVRQ
                     EREGIPVLPPHEEIRGAVELAHKEGGVHREHRNMLGGILDLRELSIGDVAIHRKNMVA
                     IDAEQPAPQLFDSLMAANHTRVPVWRETPDNIVGVLRTKDVMRELARRRGQFAGWDIL
                     SLLEEPWFVPDTTTLEEQIRAFRQRRLHFALVVDEYGALQGVITLTDIVEEIIGGIPD
                     ERGNAEQAEIRPQSDGSFQIQGATPIRDLNRVLDWHLPDAEATTIAGLVIHEARTIPE
                     VGQRFAFYGFKFEILRRQRNQITLLRVTPPQQQPPATSAA"
     gene            complement(5405..>6300)
                     /gene="aroB"
                     /locus_tag="VG274_12025"
     CDS             complement(5405..>6300)
                     /gene="aroB"
                     /locus_tag="VG274_12025"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWE07432.1"
                     /translation="TILDPGEGAKSFQGLEQLTDSLLESGLERDGLIVALGGGVIGDL
                     AGFAAGVLKRGIAYAQIPTTLLAQVDSSVGGKTAINTRHGKNLVGLFHQPKIVIADTE
                     LLATLPRRELIAGFAEIAKYGALGDPDFFAWLESQEAPLSIGEPAPLMRAVAHSCRIK
                     AAVVSQDEREAGDRALLNLGHTFGHALEAATLFSDRLRHGEAVAIGMALAFRLSAGLG
                     LSSAAATERLERHLRAMSLPVRISDIPGPRPDSDTLLFHMLHDKKSRDGRPVFVLVRG
                     IGQAFVTDDVSIEAVRAVLAG"
BASE COUNT         1202 a         1938 c         1941 g         1219 t
ORIGIN      
        1 aaagcgtccg cctgcgtatc agcatgaacg ccctgcggtc ggtcgaccgg tccggcggcc
       61 tcgacgcctt tttgatgcgc gcaaaggatg gcctgctctc cggccgcgca ctgcgcttca
      121 agcgtctcat cctgaagaag caggcagccg cctaggcggc tcgcgcacgc agccgcgagc
      181 ggccggccga gcggccgtgg aactcgtgtt cgccaaatag cgagataagc tggccggtca
      241 tggcgccgcc cagctgctcg gcatcgacga tcgtcaccgc gcgacgatag tatctcgtca
      301 cgtcgtggcc gatgccgatc gccacgagct cgatcgcgga cttctcctcg atatcctcga
      361 tcacgtgacg caaatggcgc tcgagatacg tccccgcgtt cacagacagc gtcgaatcgt
      421 ctaccggcgc gccgtcggaa atcaccatga gaatgcggcg ctgttcgagg cgtgccatca
      481 gacgctggtg cgcccaaatg agcgcttcgc cgtcgatatt ctccttgagc agaccctcgc
      541 gcatcatcaa accgagattt cggcgcgtgc ggcgccacgg ttcgtcggcg ggcttgtaaa
      601 tgatgtgcct caaatcgttg aggcgcccgg gatgcggaga cttgccctcc gcgatccact
      661 tctcgcggct ctgtccgcct ttccaggcac gcgtggtaaa tcccaggatt tcgaccttta
      721 cagcgcagcg ctcgagcgtg cgcgccagaa tatcggcgca catcgccgcg accatgatcg
      781 gccggccgcg catcgagccc gaattgtcca gcagaagtgt gaccaccgtg tcgcgaaagc
      841 tcatgtcctt ttcgcgcttg tatgtcagcg aatacatcgg atcgatgatg atgcgcggca
      901 agcgcgcggt gtcgagcaag ccctcctcga cgtcgaactc ccaactgcga ttctgctgcg
      961 cgagcaggag acgctgaagc ttgttggcaa gacgcgccac cacgccctgc atcgcctgca
     1021 gctgctgatc gaggaagttg cgcagccgtg tcagttcttc ggcatcgcaa aggtcggttg
     1081 caccgacgat ctcgtcgaac tgcgttgtat gtatgcgata cccccattcg tcctggcgcg
     1141 aaaaaggctg ttccggccgc cagggttttg tgcgggcatt atcctcttcg aggccggagg
     1201 gctcggcagt ctgttcggcg cctatttcat ccgcaagttc atcgctgtcc tgaccttcgg
     1261 attcgctttg ctcggcattt tccggctcag gacgcgcggc ggcatctgcg gtttcacccg
     1321 catcctcgga ctgcggctcg gtttcgctcg actcgtcgcc ctcgctttcg gatttctcgc
     1381 cgagatcgtc tcccatctcg aaatccttaa ggatcgcgcg ggtcagacgt gcaaacgcct
     1441 tctgatcgcg atgcacgcct tccagccgcg caaggtcctc gccggcccgt tcttcgatcc
     1501 agcttcgcca ggcatcgacg gcgggtcgcg cgcattcggg cggcggaaga ccggtgatgc
     1561 gttcgcgaac gatcaggccg atgatctccg cgagtggcgc ttcgccccgc tcgcgcgctc
     1621 tcgcgaggga acttgcgccg agccgtgctt caagtcccgc ggttagattg gcagccacgc
     1681 ctttcatggc gcgcgcgccg atcgcctcta cccgtgcctg ttcagccgcc tcgaatatcg
     1741 cggcggcatc ggcggatttc ggccggtaca tttcatgaag tttgttctca tgataggcaa
     1801 ggcggagggc gtacgcgtcg ccctggccgc gcatcacggc gatctcggac ggcggcagat
     1861 tgcgagacgg caagggcaat cgcgctttca ggcccttgag catcgaaggc tcggcactga
     1921 aattcacttc cagctcgggt tctcctgcga gcgaccgcac agcgcaagca accgcccttt
     1981 tgaacggctc ggccggagtt tcgggcttca tgccaccagg actttggctg acgattccgg
     2041 caattcgtcg ttgaaggagc gctgatagaa ctcggctacg gaggcacgct cgagctcgtc
     2101 gcacttgttg aggaaggtaa ggcggaacgc aaaacccaca tcattaaaaa tctccgcatt
     2161 ctcggcccag gtgatgaccg tgcgcggact cattaccgtg gagagatcgc cattgacgaa
     2221 cgcattacgc gtcatgtcgg ccacgcggac catcgccgaa atcagcttct tgcgctcggc
     2281 cgtgccgtaa gaaggaactt tcgcgagcac gatttcgact tccgcatcat gggtcagata
     2341 gttgagcgtg gtgacgatat tccagcgatc catctgcccc tggttgatct gctgcgtgcc
     2401 gtgatagagg cccgtggtat cgcccagacc gattgtgttc gtcgtcgaga acaaacgaaa
     2461 ggcggggtgg ggtcggatta cgcggttctg atcgagcagg gtcaaccggc cctgcacttc
     2521 gagcacgcgc tgaatcacga acatcacgtc gggccggccg gcgtcgtatt cgtcgaagac
     2581 gagtgccgta gggtgctgaa gcgcccaagg cagcagacct tcgcggaatt ccgtcacctg
     2641 ctgtccctcg cgcagcacga tcgcgtcctt gccgatgaga tcgatgcggc tgatgtgact
     2701 gtcgagattg atgcggacac aaggccagtt gaggcgcgag gcaacctgct cgatgtgggt
     2761 cgatttgccg gtcccatgat agccctggat catcacgcgg cggttgaagg caaacccggc
     2821 cagtatggcg agtgttgttt cccgatcgaa acgataagcg gagtcccgat ctggaacgta
     2881 ctcattgccg cgcgaaaagg ctggtacttt catggccgcg tcgatgccga atacggcatg
     2941 cacgtccact ttcgtgtcgg gcgtggctgt gggatcgagg ccctgtgtat ctggtatcat
     3001 ggtgcgatct ggcctggaac tcttgaggcg cacgaaatac cgcccatgcg aggcggaggc
     3061 cgccgccctc aggtcagacc agatgcccgc agcacgccgt aggcctggat aaccctcttc
     3121 agccgctcct cggcaccacg gtcgccgccg tttgcgtcgg gatgaaaccg cttcacgagt
     3181 tccttatacc gcgcctttat ctggtgcaag ctggcgccgg cttcaaggtt gagcacgtcc
     3241 agcgccagcg cctgaggccg tgtcagcggt ctctcggcac gtcgtttttc tgctgccgcc
     3301 ggatcgcccg aatcatctgc aaaaaccccg taggcgtcgc ggaaggaacg aaaaccctgt
     3361 gcgcgatgca cttgcgcggc gcgcttgccc agcggccagg tcggccgatg gccggtcacg
     3421 gcatcgacgc ggaacgcttc gatctccgat tcactcatgc cgttgaaata gtcccaatgc
     3481 tcgttgtgct ggcgcgcatg ttgcaagcag aaccacaaat gttcgtcgag ctggtcgcgg
     3541 gccttcggaa cgcgaaacga accttcgccc ttgcagctcc tggcgctaca gggtctgggc
     3601 gcgtgcgcgt ggaccggtcc atccggtttg atgcggatgt ccttgccaaa tcgcggccgg
     3661 tagcctgctt cgcccatgcc ggaattatgg gtccgagaaa tgaaaaaaca agaaaaggta
     3721 gcgcttgaca ttcgctggct tcctgcgatg gaaaaacatg agcatcgccg atgaaattca
     3781 gagaaagctt tcgcagggtc tcgcgccaac ggagctttac atcgaagatg aaagcgccca
     3841 tcacgcgggt cactcgggcg cccggccgga aggagaaacg catttccggg tgcgtgtggt
     3901 ttcgacggtc ttcgaaagca tgggccggct ggcgcgtcaa cgccttatcc acgaactcct
     3961 ttccgaagaa ctgcgcgtgc atgttcacgc gctttcgatc gaagcgcgaa cgcccaacga
     4021 agcaggttcc acgcgctcct gattttgccg gctaggcagc ggaagttgcg ggcggttgct
     4081 gctggggcgg ggttacacgc agcaaagtga tctgattgcg ttggcggcgc aggatctcga
     4141 acttgaaccc atagaacgcg aaacgctggc cgacttcggg aatcgtccgc gcctcgtgaa
     4201 taaccagacc tgcgatggtg gtcgcctcgg cgtccggcag atgccaatcg agaacacgat
     4261 tcaaatcgcg aatcggcgtt gcgccctgga tctggaacga tccatcggat tgcgggcgga
     4321 tttccgcctg ctcggcgttt ccgcgttcat caggaatgcc tccgatgatt tcttcgacga
     4381 tatcggtcag cgtgatgacg ccctgcaatg caccatattc gtcgaccacc agggcgaaat
     4441 gcaggcggcg ctggcgaaac gcgcgaattt gctcttcgag tgtggtcgtg tcgggcacaa
     4501 accacggctc ttccagcagg gaaagaatgt cccaaccggc aaactggccg cggcgccgcg
     4561 ccagctcgcg catgacgtcc ttggtgcgaa gcacgcccac gatgttgtcc ggcgtttcgc
     4621 gccacacagg gacgcgcgtg tggtttgcgg ccatgaggct gtcgaacaat tgcggggctg
     4681 gctgctcggc gtcgatcgca accatgttct tgcgatgaat tgcgacatcg ccgatcgaga
     4741 gctcgcgcaa atccagaatc ccgcccagca tgttgcgatg ctcgcgatgc actccgcctt
     4801 ccttgtgcgc gagttcgacc gcgccgcgaa tctcctcgtg cggcggcagg accgggattc
     4861 cttcgcgttc ctggcgcacg ccaaagaggc gcagtacgcg ccagaccacg aactgcacgg
     4921 agtccacaac tggcgccaga agattcacga ccggcaacag gatacggccg acgctgagtg
     4981 cgaagcggtc cgtccgggcg atcgcgagag tttttggcag cacttcggcg aacaccagaa
     5041 cgacaaaggt catcaggagc gctgtgatga tgacggccgt gttgccatag tgcgccgcca
     5101 gcagcgatgt ggccagcgac gacgccagga tgttgatgaa agtgttgccg agcaagatgg
     5161 cgccgatcat gcgctcacga tcggcgagca acatgttgac cggccgcgcg gcgcgggagt
     5221 tgcgctgctc cagccgatgc atggtcgctg gcgacacggc ggtcaatgcc gtctccgacc
     5281 ccgaaagaaa cgccgatacg gcgagcagca ctatgattgt gccgataccg atgacaagaa
     5341 aactggccat tacgatttcc tctcgcgcgg acgcttctgt ggcggaatcg tcgcggaaaa
     5401 gcacttagcc cgcaagcacg gcacgcacgg cctcgatcga aacatcgtcc gtcacgaacg
     5461 cctgcccgat tccacgcacc agcacaaaga cagggcggcc gtcgcgggat ttcttgtcgt
     5521 gcagcatatg gaagagcagc gtatcggagt cggggcgcgg accgggaatg tcggaaatgc
     5581 ggacgggcag cgacatggcg cgcagatgcc gttcgagtct ttcggtggcg gctgccgaag
     5641 aaagacccag accagcggaa aggcggaatg ccagcgccat tccgatggca acggcttcgc
     5701 cgtgacgaag gcggtcagaa aacaaggttg ccgcctcgag cgcgtggccg aacgtgtgac
     5761 ccaggttgag cagcgcgcgg tctccggctt cgcgctcgtc ctggctgacg actgcagcct
     5821 tgatgcggca ggaatgcgcg accgcccgca tgagcggtgc cggctcgcca atggacaatg
     5881 gagcctcctg cgattcgagc caggcgaaga aatccggatc tcccaatgct ccatatttgg
     5941 cgatttcggc aaagccggcg atcaattcgc ggcgcggcag cgtggccagc agctcggtat
     6001 cggcgatgac aatcttcggc tggtggaaca agccgacaag gttcttgcca tggcgcgtat
     6061 tgatcgccgt ctttccgcct acggatgaat cgacctgcgc aagcaatgtc gtggggattt
     6121 gggcgtacgc tattccgcgc ttgagcacgc cagcggcaaa acctgcaagg tcgccaatca
     6181 cgccgccgcc aagagccacg atcagaccgt cgcgttcgag accgctctca agcaacgagt
     6241 cggtgagctg ctcgaggccc tgaaagctct ttgcgccctc tccaggatcg agaattgtcg
//
LOCUS       DASZMC010000591         8797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3274492,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000591 DASZMC010000000
VERSION     DASZMC010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3274492"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VG274_12030"
     CDS             complement(<1..788)
                     /locus_tag="VG274_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HWE07433.1"
                     /translation="MFTLADINAQLRAFATDRRGIAAVYTGVVLVVLAGAAGLGADAV
                     SWELARQKLQGVADQSAYSSVIGVNDGLDGYKNAEAVATQLGYTCSSFGAPNGSGVMS
                     CTGPNGVTLNVNHPAQTGKYANVSDSWEVIVQQTQPMWFTKLFMSTAPIVAGRAVALQ
                     QGSGNVCILALDTNSTDIHSIFGNQTGTVTVGGKCQVADNLLNTSPPATDDVDVKSGA
                     ILNFAYLSMRDATKCDSGSCQGMLNVANPIQYNQAAIVDPYATRT"
     gene            complement(1227..2348)
                     /locus_tag="VG274_12035"
     CDS             complement(1227..2348)
                     /locus_tag="VG274_12035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE07434.1"
                     /translation="MKRAVLMSAASAFCLLWAAGCASTGHTDAKPVASATADKPADEN
                     PSASSDIATLAKNLPATMEGEIKRAKLLRAKGDYPEAERSLAQLMLVAPDDGIVVGEY
                     GKVLEQQGRSEEALGFLTRAVQLQPRDWTLYSALGIAYDQLDKRENARTAYERALALK
                     PGEASVLNNYAVSRMLAGDYVGAQRLLAQASAQGGDNAKIALNAEKLASISPPPPAPV
                     AVQSAPPPAVKVAVLPPAVAQAAAVKPAATQAPRPMMTVPASPAHAPIAVANLKAPRA
                     NVVMQRVPFDPLATKSASAHPGSAAGAIVSLHPVATHAPLAIVAAKSLGPNVVMQRVP
                     FDPLAGPVAAHKSVHKPAPAAAKPVDDTPSLRTAADTGN"
     gene            complement(2442..3101)
                     /locus_tag="VG274_12040"
     CDS             complement(2442..3101)
                     /locus_tag="VG274_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE07435.1"
                     /translation="MPQRYTLIVGTKQWSSWSLRAWLSMRATGAEFREITVRLRRPET
                     RAEIRRLAPADKVPVLRIEEAGAPVTVFDSLAICETLAERHTDAQLWPADFAARAEAR
                     SISAAMHSGFAVLRESLSMEFARSFPTPALSPDVEAEIAMIQNFWREALDKYGGNGGF
                     LFGRFSIADCMYAPVVSRFRTYGIALDPVVDAYCDRIWALPAMQDWLVAAKTELESGL
                     C"
     gene            3274..4668
                     /locus_tag="VG274_12045"
     CDS             3274..4668
                     /locus_tag="VG274_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HWE07436.1"
                     /translation="MHSSLVPHARNAVPLDAVCAVNVEKWLRRRKGREANWLRRTGFT
                     AKEGQIALVPSAGGEIAFAVLGLGNEFDPLALAAFPESLPRGAYALRDVPAEFGSARA
                     TLAWLLGTYSFDRYRKRRKQFPKLVVPPGVDAADATRIAEGVFLARDLVNTPSNDMGP
                     AELADAARALAARHHAKISIVAGGDLLKQDYPLIHAVGRASERAPLLIDLRWGSDKAP
                     KVTLVGKGVCFDSGGLDLKNSAGMLTMKKDMGGAAVMLAVAQMAMDAKLSLRLRVLVP
                     AVENAVSGRAYRPGDVFPSRKGLTVEIGNTDAEGRLVLADALCEADSESPELLIDVAT
                     LTGAARSATGMEVPPFFTDDENLAADLARHAAAVHDPLWRLPLWRGYERTLASPIADL
                     TNNPDYNLAGAITAALFLNRFVERARSWVHLDIPAWIDRPRPGRRRGAEANAARALYA
                     LLAERYPAKTVRRR"
     gene            complement(4862..5809)
                     /locus_tag="VG274_12050"
     CDS             complement(4862..5809)
                     /locus_tag="VG274_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HWE07437.1"
                     /translation="MTASTLLLLSPEPLIDLFLGPLVETAPSNLRVVVHGRDQYAVPV
                     IDFLLSFRPPKGFAASLPNLKAVFSMGAGVDAFLADPDFPRHVPLVRLIDQTLSRDMT
                     EYAVLQILTQHRQQGFYDAAQHERVWRQSIPARRTESTRIGIMGIGEIGTFMGMRLRD
                     LGFAVSGWSTSRKHIDGIESFAGPAELREFLARSDFLVCVLPLTPGTRGILDRETFAM
                     LPRGAFVINIARGEHLVEADMIAALDSSHLSGAALDVFVTEPLPATSPLWCHPKIVVT
                     PHIAALSDPRAVARNVAEGIARLERGEPLLHVVDPARGY"
     gene            complement(5820..6485)
                     /locus_tag="VG274_12055"
     CDS             complement(5820..6485)
                     /locus_tag="VG274_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HWE07438.1"
                     /translation="MDSFEWNKIIGAVLGTLLFVVASKIISEMIFEVPPPAKPGYVVQ
                     GVPAAATGGPAQPAEEPLPDFGTVLAKADTAKGQQISQRCQQCHDLSKGGPDKIGPNL
                     WGVVGRPRASRASFSYSSAMSTSHAPWTFENLFRYLKSPQTVVPGTKMSFAGLPNAQD
                     RIDLIAWLRTQSDSPVPIPPPKPAAAPSGAKPAATPAKPTGAAPAGAKPAAPAKPAPT
                     KPG"
     gene            6644..7396
                     /locus_tag="VG274_12060"
     CDS             6644..7396
                     /locus_tag="VG274_12060"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HWE07439.1"
                     /translation="MNPIVIIPARMASTRLPGKPLADISGTPMIVRVWRQAMAAGIGP
                     VLVAAAEAEIAHAIENAGGAAVLTDPALPSGTDRVFAALETVDVKGAHDVIVNLQGDL
                     PLLDPAQLRNVVEALAASGADIATLAAPVIDEADFDNPNVVKPVIAWNTDGSLGRALN
                     FTRARAPYGPGAVFHHIGIYAFRRAALARFVALPPSPLEQREKLEQLRALEAGMSIAV
                     ARIDKVPLSVDTEADLARVREAVARDAGVSPG"
     gene            7393..8265
                     /locus_tag="VG274_12065"
     CDS             7393..8265
                     /locus_tag="VG274_12065"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWE07440.1"
                     /translation="MSASSLTARATRVAFQGEPGAYANLAAREALPHAMAIPRPTFED
                     AVSAVRDGEAGLAIIPVENSLHGRIADIHHILPDAGLYIVGEHFHRVRHQLLGLKGAT
                     LLGLKTVLSQGPALGQCRKIIRDMKLGARIWHDTAGSARHVAELRDPTVAAIASSLAA
                     EIYGLDILKADVEDESHNMTRFLIMAREPDHAAMGSGPVVTTFIFRVRNVPAALYKAL
                     GGFATNGVNMTKLESYQLGGSFNATQFYADIEGHPEERKVRLALEELQFFTSTLTVLG
                     VYPAHPFRGEMPEG"
     gene            complement(8313..>8797)
                     /locus_tag="VG274_12070"
     CDS             complement(8313..>8797)
                     /locus_tag="VG274_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HWE07441.1"
                     /translation="LQLEAIVNAANEPLIMGGGVDGAIRRKAGPEMEAELRRIGRCAT
                     GKAVLTRGHRLPAKFVIHTVAPVWHEEAATREEKLRLLGDCYRNALTVAQANGMSEIA
                     FPCLGTGIYGWPADLAARTAFDAVVAFLRNDEVLSRIVFCCFSAADMNRYAALIAALK
                     "
BASE COUNT         1560 a         2819 c         2865 g         1553 t
ORIGIN      
        1 gtgcgggtgg cataaggatc gacgatcgcg gcttgattgt attgaatcgg gttcgcgacg
       61 ttcagcattc cctgacagct gccgctgtcg catttggtgg cgtcccgcat cgaaaggtag
      121 gcgaagttga gaatggcgcc cgacttgacg tcgacgtcat cggtagcggg gggggaggtg
      181 ttcaacaggt tgtcggcgac ctgacacttg ccgccgaccg tgaccgttcc ggtctggttg
      241 ccgaaaatgc tgtgaatatc ggtgctgttg gtatcgagcg cgaggatgca gacattgccc
      301 gagccctgtt gcagggcgac cgcacggccc gccacgatgg gagctgtcga catgaacagc
      361 ttggtgaacc acatcggctg cgtttgctga acgatgacct cccacgagtc gctgacattg
      421 gcgtatttgc ccgtctgtgc agggtggttg acgttgagcg tgaccccgtt gggtcccgtg
      481 cagctcatca cgcccgatcc gttcggcgcc ccgaagctgc tgcaggtata gcccagctgg
      541 gttgccacgg cctccgcgtt cttgtagccg tccaatccgt cgttcacgcc gatgacggag
      601 gaataggctg actgatcggc cacgccctgc aatttctgcc tggccagctc ccacgagacg
      661 gcatccgctc ccaagccggc agcgccggcg aggacgacca gcaccacccc ggtatacacc
      721 gcggcgatgc cgcggcgatc ggtggcgaac gcgcgcagtt gggcattgat gtcggcaagc
      781 gtgaacattc caatcactcc gatacctgat cttgcgcggg gaagatggga cgaatttgtt
      841 gactaagcat gaacgtacaa tcctgcaggc ccaagatcag tcgttttgta cggacatgct
      901 taactggatt taaaacaccg ccatgaattc gctttaactc tgcctatctc aatctgaaac
      961 actaagacga atgtcggcga aactcgtctt taccttcttc tgcggaccac agatcgacgc
     1021 cttcaccctc tctgttcgca atcatcctcg accccgccat cgaaaacatc acgcagacga
     1081 cggaactctg cgcgctgatt tcccggcgtt gatgcaagat tccgattccc ctctcccctt
     1141 gcgggagagg ggtgccagca cgggcggtta gtcctgttca ggcgcgggtg agggggagca
     1201 cggcgcgtgc gcaggcggcg ctcgcctcag ttgccggtgt cggcggcggt gcggagggag
     1261 ggagtgtcgt ccacgggttt ggccgcggcg ggcgctggct tgtgcacgga tttgtgcgct
     1321 gcgaccggtc cggcgagagg atcgaacggc acgcgttgca tgacgacgtt tggtcccagg
     1381 gatttcgcag ccacgatcgc gagcggcgca tgcgtcgcga ccgggtgcag cgaaacaatc
     1441 gcgcccgctg cgcttccggg atgcgcactc gcgctcttcg tcgcgagagg atcgaacgga
     1501 acgcgctgca tcaccacatt tgcccggggc gctttcagat tggccaccgc aattggagca
     1561 tgggcaggcg aagcaggcac ggtcatcatt ggtcttggcg cctgcgtggc cgcaggcttc
     1621 acggccgccg cctgcgctac ggcgggcggg agcactgcaa ccttgacggc cggcgggggt
     1681 gcggactgca cagccacggg cgccggcggc ggcggactga tcgacgcgag tttctcagcg
     1741 ttcagcgcga tcttggcatt gtcgccgccc tgcgccgaag cctgggcgag caggcgttgc
     1801 gcgcccacat aatcgccggc gagcatccgc gataccgcat aattgttgag caccgaagcc
     1861 tcgcccggct tcagcgccag cgcgcgctca taagccgtgc gcgcgttttc gcgcttgtcg
     1921 agctggtcgt aagcgatgcc gagggcggaa tagagcgtcc agtcgcgtgg ctgcaattgc
     1981 acggcgcgcg tcaggaagcc caaagcttcc tcggagcggc cttgctgttc cagcaccttg
     2041 ccgtattcgc cgacgacgat tccgtcgtcg ggcgccacga gcattagttg cgcgagcgaa
     2101 cgctccgctt cggggtagtc gcccttggcg cgcaacagtt tggcgcgctt gatctcgcct
     2161 tccatcgtgg ccggaaggtt cttggccagc gttgcgatgt cggaactggc gctggggttc
     2221 tcgtccgcgg gcttgtccgc ggtggcggaa gccacgggct tggcgtccgt atgccccgtg
     2281 ctcgcacacc ccgcggccca tagaaggcag aatgcggatg cggcgctcat caggacggcg
     2341 cgtttcatgg ctggcctcgg cttgaatgtc cgggacccag aatgatcggt tcgggtcaat
     2401 aattgattaa gcctgttggc ggcaatcgaa gaaagcgcgc ctcagcagag gccggattcg
     2461 agctcggtct tcgcggcaac cagccagtcc tgcatcgcgg gcagcgccca tatgcggtcg
     2521 caataggcat cgacaacggg atcgagcgcg atgccatagg tgcggaagcg tgaaacgacc
     2581 ggcgcataca tgcaatcggc gatcgagaat ctgccgaaca ggaatccgcc gttgccgccg
     2641 tatttgtcca gcgcctcgcg ccagaaattc tggatcatgg caatctccgc ctcgacgtcg
     2701 gggctcagcg cgggcgtggg aaagctgcgc gcgaattcca tcgaaaggct ttcgcgcagc
     2761 accgcaaagc ccgaatgcat tgccgcgctg atcgagcgcg cctcggcgcg cgccgcaaaa
     2821 tcggctggcc agagctgcgc gtccgtgtgg cgctcggcga gcgtctcgca gatggcgaga
     2881 ctgtcgaaca cggtaacggg cgcgcctgcc tcctcgattc gcagcacggg caccttgtcg
     2941 gcgggcgcga gccggcgaat ctccgcgcgc gtttcgggcc gcctcagtcg cacggtgatc
     3001 tcgcggaatt ccgcccccgt cgcacgcatc gagagccagg ctctcagact ccagctcgac
     3061 cattgtttcg tgccgacgat caacgtgtag cgttggggca taggggactc caaggccggg
     3121 gaaggggacg gcgctgatcg caaacgtttc tattcggcca gttggtgttc cagtatcacc
     3181 ttttgacctt tattgactca tgtgtcggcc gtttgtgtca ctcgtcattg tccaggtgtt
     3241 agattgctcc gccaacgcag caaaggttcg atcatgcatt ccagccttgt gccgcacgca
     3301 cgaaacgccg tgccgctcga cgcggtgtgc gcggtgaatg tggaaaaatg gctgaggcgg
     3361 cggaaaggcc gcgaagcaaa ctggctgcgt cgcacgggtt tcaccgcaaa ggaaggccaa
     3421 atcgcgcttg tgccttccgc gggcggcgag atcgcctttg cagtgctggg actcggaaac
     3481 gagttcgatc ccctcgctct cgccgccttt ccggaatcct tgccgcgcgg cgcctatgcc
     3541 ttgcgcgacg tccccgcgga attcggcagc gcgcgcgcga cgctcgcctg gctgctcggc
     3601 acctacagtt tcgaccgcta caggaagcgg aggaagcaat ttccgaaact cgtcgtaccg
     3661 ccgggcgtcg acgccgcaga cgccacgcga atcgcggaag gcgtgttcct cgcgcgcgat
     3721 ctcgtcaata cgccgtccaa cgacatgggg ccggcggagc ttgcggacgc ggcgcgcgcg
     3781 cttgccgcgc ggcatcacgc caaaatctcg atcgttgcgg gcggcgacct gctcaaacag
     3841 gactatccgc tcatccatgc ggtgggccgc gcctcggagc gcgcgccgct tctgatcgat
     3901 ctgcgctggg gcagcgacaa ggcgccgaag gtgacgctgg tcggcaaggg cgtctgcttc
     3961 gattcgggcg ggctcgatct caagaactcc gccggcatgc tgaccatgaa gaaggacatg
     4021 ggcggcgcgg ccgtgatgct cgccgtcgcg cagatggcga tggacgcgaa gctgtcgctg
     4081 cgcctgcgcg tgctcgttcc ggcagtcgag aatgccgtgt ccggccgtgc ctatcgcccg
     4141 ggcgatgtgt tcccgagccg caagggactg accgtcgaga tcggcaacac cgatgcggaa
     4201 ggacgcctcg tgctcgccga tgcgctttgc gaggccgaca gcgaatcgcc cgagctgctg
     4261 atcgacgtcg ccacactcac cggcgcggcg cgctcggcca ccggcatgga agtgccgccg
     4321 ttcttcaccg acgacgaaaa cctcgcggcc gatctcgccc ggcatgctgc ggcggtgcac
     4381 gatcccttgt ggcgcctgcc gctctggcgc ggctacgagc gcacgctcgc gagtccaatc
     4441 gccgatctca ccaacaatcc cgactacaac ctcgccggcg cgattaccgc agccctgttc
     4501 ctgaaccgct tcgtcgagcg cgcgcgttca tgggtgcacc tcgacattcc ggcgtggatc
     4561 gaccggccgc gccccggccg ccggcgcggc gccgaggcca acgccgcccg cgcactctac
     4621 gcactgctcg ccgagcgcta tcctgcgaaa acagtacggc ggcgataaac acatcgagtc
     4681 cgagctttaa ttgttcctgt catggcccac gcaagcgggc cacccagctg aagcccgcaa
     4741 tgcattgcgc gttcacgaaa gaagtcctgc gtgctttcaa ccgcgcgccg tttcgactgg
     4801 gtggcccgct cttcggcggg ccatgacaag ttttttggtt ttggtcgact cagaatcggc
     4861 actaataccc ccgcgcggga tcgacgacgt gaaggagcgg ctcgccgcgc tcgagacggg
     4921 caatgccctc ggcgacgttg cgcgcgacgg cgcgtggatc ggacaaagct gcgatgtgcg
     4981 gcgtgacgac gatcttggga tggcaccaca gcggactcgt cgcgggcagc ggctccgtga
     5041 cgaagacatc gagtgccgcg cccgaaagat gagacgaatc gagtgctgcg atcatatccg
     5101 cttcgacgag atgttcgccg cgcgcgatat tgatgacgaa cgcgccgcgg ggaagcatcg
     5161 cgaaggtttc gcgatcgagg atgccgcgcg tccccggcgt cagcggcagc acgcagacga
     5221 gaaaatcgct tcgcgcgaga aattcgcgca attctgccgg acccgcgaag ctttcgatcc
     5281 cgtcgatgtg tttgcggctc gtgctccagc ccgagacggc gaaacccaga tcgcgcaggc
     5341 gcatgcccat gaaggttccg atttcgccga tgcccatgat gccgatgcgc gtggattccg
     5401 tccgccgcgc cgggatcgac tggcgccaga ctctttcgtg ctgggctgcg tcgtaaaatc
     5461 cctgctggcg atgctgcgtc aggatctgca gcaccgcata ttcggtcatg tcgcgcgaca
     5521 gggtctgatc gatgagccgc accagcggca catggcgcgg gaaatccggg tcggcaagga
     5581 atgcatcgac accggcgccc atcgagaaca cagccttcaa gttcggcagc gaggcggcga
     5641 agcctttcgg cgggcgaaag ctcagaagaa agtcgatcac agggacagca tactggtcgc
     5701 ggccgtgaac gacgacgcgc agattcgacg gcgccgtttc gacgagcggt ccgaggaaca
     5761 ggtcgatcag cggctccggc gagagaagca gcagcgttga tgcggtcaag acgagttcgt
     5821 caccccggtt ttgtcggcgc gggtttggcg ggcgcggcgg gttttgcccc cgcaggagcg
     5881 gcgcctgtgg gcttggcagg agtcgccgca ggctttgcac cggacggagc ggccgcgggt
     5941 ttcggcggcg gaatcggcac gggcgaatcg gactgcgtgc gcagccaggc gatcagatcg
     6001 atgcgatcct gcgcgttcgg cagtccggcg aaagacatct tcgttccggg caccacggtc
     6061 tgcggagatt tgagataacg aaacaggttc tcgaacgtcc agggcgcgtg gctcgtcgac
     6121 atcgcgctcg aataggagaa gctcgcgcgc gaggcgcggg gccgcccgac gacgccccac
     6181 agattgggac caatcttgtc gggaccgcct ttggagagat cgtggcattg ctggcatcgc
     6241 tgcgaaatct gctggccttt cgcggtatcg gccttggcga gcacggtgcc gaaatccggc
     6301 aaaggttcct ccgcgggctg ggccggaccg cccgtggccg cggcggggac tccctgcacc
     6361 acatagccgg gctttgcagg cggcggcact tcgaagatca tctcgcttat gatcttggac
     6421 gcgactacga acaggagcgt gccaagaacc gcgccaatga tcttgttcca ctcgaaggaa
     6481 tccataaggt ctggctccac gtcccgtaac ggctgacagc aagaaacggc gcaagacgcg
     6541 cgccgacgat cccccgaagc gggcgcgaca ttacaagccg tccccgcaac gtcattgcgg
     6601 cgaagcgtcg cataagttgc ggatgcggca agagaaaaga cacatgaatc ccatcgtgat
     6661 cattccggcg cgcatggcct cgacgcggct tcccggaaag ccgctcgccg acatctccgg
     6721 cacaccgatg atcgtgcgcg tgtggcggca ggcgatggcg gccgggatcg ggccggttct
     6781 cgttgcggcg gcggaggcgg aaatcgcgca tgcgatcgaa aacgcaggtg gcgctgccgt
     6841 gctcaccgac cctgctctgc cgtctggcac cgatcgcgta ttcgcggcgc tcgagaccgt
     6901 cgacgtcaag ggcgcgcacg atgtgatcgt caatctgcag ggcgacctgc cccttctcga
     6961 tccggcgcaa ttgcgcaacg ttgtggaggc tctcgcggca agcggcgccg atatcgcgac
     7021 gctcgccgca ccggtcatcg acgaagcgga tttcgacaat ccgaacgtgg tcaaacccgt
     7081 tatcgcctgg aacacggacg gatcgctggg ccgcgcgctt aacttcacgc gcgcgcgcgc
     7141 gccttacggc cccggcgcgg ttttccatca catcggcatc tatgcgttcc gtcgcgctgc
     7201 gcttgcgcgc ttcgtcgcgc tgccgccatc gccgctcgaa cagcgagaaa agctcgaaca
     7261 gcttcgcgcg ctcgaagccg ggatgtcgat cgcggtcgcc cgtatagata aagtgccact
     7321 ctccgtcgac accgaagccg atcttgcgag ggtgcgagag gctgtggcgc gcgatgcggg
     7381 agtctcgcca ggatgagcgc ctcgtcgctg accgcgcgcg cgacgcgcgt ggcgtttcag
     7441 ggcgagcccg gcgcttacgc caacctcgcc gcgcgcgaag ccctgccgca cgcgatggcg
     7501 atcccgcgcc cgacgttcga ggacgcggtc tccgcggtgc gcgatggcga ggccggtctc
     7561 gcgatcatcc cggtcgaaaa ctccctgcac ggacgaattg ccgatattca tcacatcctg
     7621 cctgacgcgg gcctctacat cgtgggcgag catttccatc gggtgcgcca ccagttgctc
     7681 gggctgaaag gcgcgacgct cctcggactg aagaccgtac tgagccaggg accggcgctc
     7741 ggccagtgcc gcaaaatcat ccgcgacatg aagctcggcg cccggatctg gcacgacacc
     7801 gcgggatcgg cgcggcacgt cgcggaactg cgcgatccaa ccgtcgccgc gatcgcctca
     7861 tcgctcgccg cggaaatcta cgggctcgac attctcaaag ccgacgtcga ggacgaatcg
     7921 cacaacatga cgcgctttct catcatggcg cgcgagccgg accacgcggc gatgggctcc
     7981 ggcccggtgg tgacgacttt cattttccgc gtgagaaacg ttcccgccgc gctctacaag
     8041 gcgctcggcg gcttcgccac caacggtgtc aacatgacga agctagagag ctatcaattg
     8101 ggcggctcat tcaatgcaac gcaattctac gccgacatcg aaggccatcc cgaagagcgc
     8161 aaggtgcgcc tcgcactcga ggagctgcag ttcttcacct cgacgctcac cgtgctcggc
     8221 gtctaccccg cccatccctt ccgcggagaa atgccggagg ggtgatgcag agtctgtcgg
     8281 cagatcgcac ctcgcagcag ctagcggcaa catcacttca gcgcggcaat gagcgctgcg
     8341 tagcgattca tatcggcggc gctgaagcag cagaagacga tgcgcgaaag cacctcgtcg
     8401 ttgcgcagaa acgcaacgac ggcgtcgaac gccgtccgcg cggcgagatc ggcgggccag
     8461 ccgtagatgc cggtgccgag gcagggaaag gcgatttccg acattccgtt cgcctgcgcg
     8521 actgtgagcg cattgcggta gcagtccccg aggagacgaa gcttttcttc gcgcgtcgca
     8581 gcttcctcgt gccagacggg cgcgacggtg tggatcacga atttcgccgg cagacgatgg
     8641 ccgcgcgtga gcaccgcttt tcccgtcgcg cagcggccga tgcggcgcag ctccgcctcc
     8701 atctccggtc cggccttgcg gcggatcgcg ccatcgacgc cgccgcccat gatcagcggc
     8761 tcgttcgccg cattgacgat cgcctcgagc tgaagcc
//
LOCUS       DASZMC010000592         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3275283,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000592 DASZMC010000000
VERSION     DASZMC010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3275283"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VG274_12075"
     CDS             <1..957
                     /locus_tag="VG274_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWE07442.1"
                     /translation="FRDAVALKLERDNGLKFDPATEILATSGATFGIHAALTALIEEG
                     DEVMLPDPIYDAYHSPILLAGGLIRPVSSTVTNGRFSIGLEALERAWIPSVKALILNT
                     PWNPVGSVMTEDELRAIGYFCRRRNVTLISDEIYEAIAYDGHKHISPLAACPDLRDRC
                     VLVNSLSKTYAMTGWRVGYCAGPKEIIQRMFMVLAQSSRGPATFVQDAAAEALTSSQE
                     CVAAMRDEYARRREEVLRALSGLEYVTVAPPEGGFFAMADVSALRRPSNQIRRRVMHD
                     NGVVVIHGAAYGPGGEGTLRISFATGGDTLRTGLARLREGLR"
     gene            954..>2576
                     /locus_tag="VG274_12080"
     CDS             954..>2576
                     /locus_tag="VG274_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWE07443.1"
                     /translation="MTGTVADFEAALKGEIEGEVRFDKISRALYSTDASVYRIEPIGL
                     VIPKSREDIVRAAAICRRFQCPITMRGGGTSQAGQAIGEGLQIDTSKYYSAVIELNTE
                     ERWVRVEPGIVLDELNAQLAPLGLRFAPDISTASRASIGGMMANNSSGARSVLYGKTI
                     DHVLEQTVLLSDGTVAHFRDLSRESLPGGDTLEAACYRTVLQLASQHAEEIDRRYPKI
                     LRRVAGYNLDEFTDPSRPVNLARMMVGSEGTLGLVLEAKLRLVPLPKAKAVMVILFGD
                     LLESLAAAPAILRHNPSAIEVMDKAILDNTRQNAGLEKIRDSFVKGDPASTLCVEFYA
                     DRKEDLPARLAALESDLRARGYGYHYHSETDPAAQAKIWSLREAALGLSMASREDAKS
                     ISFVEDTAVAPEKLSAFIGEFLKIVAAHNTTAGIYAHASVGCLHVRPVVNLKTAEGVA
                     NFESIANAVADLVLNYGGALSGEHGDGLVRSPFMERMFGPVLYSAFREIKKTFDPEGL
                     FNPGKIVDAPSMTANLRYGPGYKTTQPFTFFDY"
BASE COUNT          517 a          814 c          761 g          484 t
ORIGIN      
        1 tttcgcgatg cggttgccct gaaactggag cgcgacaacg gcctcaaatt cgacccggcc
       61 accgaaatcc tcgcgacatc cggagccaca ttcggaattc acgcagccct caccgcgctg
      121 atcgaagagg gcgacgaagt aatgctgccg gatccgattt acgacgcata tcattcgccg
      181 attctgctgg cgggcggcct gatccggcct gtcagttcaa ccgtaaccaa cggacgattt
      241 tcgatcggcc tcgaagctct tgagcgcgcc tggattccga gtgtaaaggc cctgattctg
      301 aacacgcctt ggaatcccgt gggcagcgtt atgaccgaag acgaattgcg cgcgatcgga
      361 tacttctgcc gccgccgcaa cgtgacgttg atctccgacg aaatttacga agccatcgcc
      421 tacgacggtc acaagcacat ttccccgctc gccgcctgcc cggatttgcg ggaccggtgc
      481 gtgttggtga acagcctttc gaagacgtac gctatgacgg gttggcgcgt cggctactgc
      541 gcgggtccga aggaaatcat ccagcgcatg ttcatggtgc tggcgcaatc gagccgcggg
      601 cctgcaacat tcgttcagga cgccgccgcc gaagcgctca cgagttccca ggaatgtgtg
      661 gccgcgatgc gcgacgaata tgcccgccgc cgcgaagagg ttttgcgcgc gctcagcgga
      721 ctcgaatacg tcacggttgc gccccccgaa ggcggattct ttgcgatggc ggacgtgagc
      781 gccctaaggc ggccatccaa ccaaattcgc cgaagagtaa tgcacgacaa cggcgtggtc
      841 gtcattcacg gcgctgccta tggcccgggc ggcgaaggaa cgctgcgcat atcgttcgca
      901 accggcggcg acacactgcg cacgggactg gcgcggcttc gcgaaggctt acggtgaccg
      961 gaacggttgc ggatttcgaa gcggctctca agggcgaaat cgaaggcgaa gttcgcttcg
     1021 acaaaatttc gcgcgcgctt tattccaccg acgccagcgt ttaccggatc gagccgatcg
     1081 gcctcgtgat accgaaatcc cgcgaagaca tcgtgcgggc cgccgcgatc tgccggcgtt
     1141 ttcaatgtcc catcacgatg cgaggcggcg gcacgtcaca ggccggccag gccatcggcg
     1201 aaggccttca gatcgatacc tcgaaatact acagcgcggt catcgaactg aacacggagg
     1261 aacgctgggt gcgcgtagag ccgggcatcg tgctcgatga gctgaacgcg caactcgcgc
     1321 cactcggtct ccgcttcgcg cccgatattt ccacagccag ccgcgccagt atcggcggca
     1381 tgatggcgaa taactcgagc ggcgcgcgca gcgtgctcta cggcaagacg atcgatcacg
     1441 tgctggaaca gaccgtcctg ctttcggacg ggaccgttgc gcattttcgc gatctctcgc
     1501 gcgagtcgtt gccaggcggc gacacgctgg aagcggcatg ctatcgaacc gtccttcagc
     1561 tcgcgtcgca gcatgccgag gagatcgatc ggcgttatcc gaaaattctc cgccgcgtgg
     1621 caggctacaa cctcgatgaa ttcaccgacc catcgaggcc tgtgaatctg gcccgaatga
     1681 tggtcggctc ggaaggcaca ctgggcctcg tgctcgaagc caaactgcgc ctggtgccgc
     1741 tgccgaaagc aaaggcggtg atggtgattc tttttgggga tctgctcgaa tcgctggccg
     1801 ccgctcccgc gatcctgcgg cacaacccat ccgcaatcga agtgatggat aaggccatcc
     1861 tcgacaacac acggcagaac gccggtctcg agaaaattcg cgacagcttt gtcaagggcg
     1921 atccggcatc cactctatgc gtcgaatttt atgcggatcg caaggaagat ctgcccgccc
     1981 gcctcgccgc gctcgaaagc gatctccgcg cccgcggtta cggctaccac tatcactcgg
     2041 aaaccgatcc cgccgcgcag gcgaaaatct ggagcctgcg cgaagcggcg ctgggtcttt
     2101 ccatggcgtc gcgcgaggac gcgaaatcca tttcatttgt cgaagacaca gcggttgccc
     2161 cggaaaagct gagcgccttc attggcgaat tcctcaaaat cgtcgctgcc cacaacacga
     2221 ccgcgggaat ttatgcgcac gcgtccgtcg gttgcctgca cgtgcgcccg gtcgtaaacc
     2281 tgaagacagc ggagggcgtc gccaatttcg aatccatcgc caacgccgtg gccgatcttg
     2341 tgctcaatta cggcggcgcg ctttccgggg agcatggcga cggccttgtg cgcagtccgt
     2401 ttatggagcg gatgttcggt cccgtgctgt actccgcctt ccgcgagatc aaaaagacgt
     2461 tcgatcccga aggtctcttc aatcccggca agatcgtgga cgctccctcg atgaccgcga
     2521 acctgcgtta cggccccggc tacaaaacca cccagccttt cacgttcttc gactat
//
LOCUS       DASZMC010000593         4352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4096696,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000593 DASZMC010000000
VERSION     DASZMC010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4096696"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VG274_12085"
     CDS             complement(<1..303)
                     /locus_tag="VG274_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07444.1"
                     /translation="MDVPEDRTYIELVARTDDAFGKRQYCEDQIDRVVAQLSAILSPQ
                     LFEMEIWSGWLCDQSRLFGDSWLMMVRPVTFNASDVEAHIKNFKRAVTGDSEIDGRF"
     gene            complement(500..>4352)
                     /locus_tag="VG274_12090"
     CDS             complement(500..>4352)
                     /locus_tag="VG274_12090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWE07445.1"
                     /translation="TRILYVSPLKALSNDVRVNLDIPLAGIAELARAKGTPLPEIRAA
                     VRTGDTPMAERALMTRKAPHILVTTPESLFILLTAGKSREMLRSVRTLIVDEIHAVAG
                     SKRGSHLALSIARLEALAEGRVQKIGLSATVSPIEEVGRFLSDRAHIIQVGRRRDMDI
                     AVEVPRDELGAVASNEMWSEIYDRTAALIGEHRTTLVFVNTRRQAERIAHALGERMGE
                     GVVLPHHGSLSRRLRLEAEQRLKRGELRAVVATASLELGIDIGTVDLVCQMGSPRSIS
                     VALQRVGRAGHWVGAKPKGRIFATTRDELIESAAVVRSIKRGELDRLEIPRAPLDILA
                     QQIVAMAACDDWDEDALFAFVRGVDPYRDLTRRDFDAVVEMLSEGMATSRGRRGAFLH
                     RDQVNHRLRGRRGARLMAITNGGAIPDSAQYQVVAEPEGTVVGTIDEDFAVESLAGDV
                     FLLGTTSWRIKRVETGRVRVEDAHGAAPTIPFWRGEAPGRTRELSNAVSELRVEIAGG
                     DVAAAIDMLMSECGLDRLGAEQAVEYVRAGARSLGAVPSEDTVVAERFFDEAGGMQMI
                     VHAPFGSRINRAWGLALRKRFCRAFNFELQAAATDNGLVLSLSDQHSFPLEIIFSYLR
                     PDTVTKVLTDALLAAPMFGARWKWNASRALAVPRFMGGRKVPPPIQRMRADDLLAAVF
                     PDQAACAENLVGEIRIPDHPLVNETIDNCLHEAMDLNGLIAILERMEEGTLKTVAIDT
                     PEPSVFSHEILNANPYAFLDDAPLEERRTRAVQLRRGLPPADATGIGALDPAAIAEVS
                     EQSWPVVRDADELHDALLTLVLLPPVEEWRRYYDELARAGRASTVDGHWMATERLDRT
                     GDTLAVLRGWTDSIGPFTAAGLAARLSLPVSDVEIALAQLEAEGLILRGNFTGADHEF
                     CHRRILARIHRQTLGRLRREIEPVSSADYMRFLCRWQHLSPGARLHGVDGLYQVLKQL
                     QGYEISAAAWESTVLPRRVHKYDPEWLDRLCLAGEVMWGRLSPHPSLETSGSRKVRPT
                     RVAPLGIFLREDAARLLPAASEVQQSWSHAAKEVLAALEQRGASFFHDLVRATGRLAS
                     EVEDGLWELVAGGRVTADGFENLRALIDPKRRRGEGRGRLARPRHAAGRWAILETGAT
                     GTDVEFYARMLLDRWGVVFRDVLARETLAPPWRSLLVVLRQWEARGEVRGGRFVAGFS
                     GEQFARPEAVELLRDLRRTSRDNGGQGEEIEIALADPLNLTGVILPGPRTSALAQGVL
                     RLRDGVQQEEPSPMPGISISA"
BASE COUNT          820 a         1534 c         1228 g          770 t
ORIGIN      
        1 gaagcgtcca tcgatctccg aatcgccggt caccgcacgt ttaaaattct tgatatgagc
       61 ctcgacatcg ctagcattga aggtcacggg gcggaccatc atgagccacg aatctccaaa
      121 aagtcggctt tgatcgcaca accatccgct ccaaatctcc atctcgaata gttgaggaga
      181 tagaatcgcc gacagttgag cgacgactcg atcgatttga tcctcgcaat attggcgttt
      241 gccaaaggca tcgtcggtac gagcaacaag ttcgatatat gtacgatctt ccgggacgtc
      301 catgcgttga ggctttagga gtgtgcttgg cttcccgccc gcgaatacac gaggtactac
      361 cgggaccaat acgttatatt gccgtggtcc ccgaaacact tcgattcgaa ctggatccgc
      421 taccactttt ccatggtctc ccagcacgag ttcggtccag taaagcttca tgaatctgta
      481 aaattgcgac atagatcgcc tacgccgaaa tagaaatccc cggcatcggc gacggctctt
      541 cctgctgtac gccatcccgc aagcgcaaca ccccttgcgc gagcgcactg gtgcgcggac
      601 cgggcagaat cacgccggtc aggttgagcg ggtcggcgag cgcgatttca atctcttcgc
      661 cttgaccgcc gttgtcccgt gaggtgcggc gcagatcgcg cagcagttcc acggcctccg
      721 ggcgcgcgaa ctgctcaccg gaaaatcccg cgacgaatcg tccgccgcgc acctcgccgc
      781 gagcttccca ttgccgcagc actaccagca ggctgcgcca cggcggcgcc agcgtttcgc
      841 gcgccaatac atcgcggaac accacgcccc agcggtcgag aagcatccgc gcgtagaatt
      901 cgacgtcggt tccggtcgcg cccgtttcca gaatcgccca ccgtcctgcc gcatgccgcg
      961 gacgcgccaa ccgtccgcgt ccctctccgc gccgccgctt cgggtcgatc aatgcgcgca
     1021 gattttcgaa cccgtcagcg gtgacgcggc cgccggccac cagttcccac aatccgtctt
     1081 cgacttcgct tgccaagcgc ccggtggcgc gcaccaggtc gtggaagaag gaagctccgc
     1141 gctgttccag cgccgccaga acttccttcg ctgcatgcga ccaactctgc tgcacctcac
     1201 tcgccgccgg cagcaatcgc gccgcgtctt cgcgcagaaa aattcccagc ggagccaccc
     1261 gcgtcgggcg caccttgcgg ctccctgaag tttctaatga cggatgcggc gacagccgtc
     1321 cccacatcac ttcgcccgcc aggcatagcc ggtccagcca ctcgggatcg tatttatgca
     1381 cgcgcctcgg cagcaccgtc gattcccacg cggcggcgga aatttcgtaa ccctgaagct
     1441 gtttaagaac ctgatagagg ccgtcgacgc catgcagtct cgcgcccggg ctgaggtgct
     1501 gccagcggca caggaagcgc atgtaatcgg cggagctgac gggctcgatt tcgcggcgta
     1561 atcgccccag cgtctgccgg tggatgcgcg ccaggatgcg gcgatgacag aactcatgat
     1621 ctgcgccggt aaaattcccg cgcaggatca gcccttcagc ttcgagctgc gcgagcgcga
     1681 tttccacgtc gctcacgggc aagctaaggc gcgccgccag ccccgccgcc gtgaacggac
     1741 caatcgaatc cgtccatccg cgcagaaccg ccagcgtgtc accggtacga tccagacgct
     1801 cggtcgccat ccaatgtcca tcgacggtcg atgcgcgtcc cgcccgcgcc agttcatcgt
     1861 agtacctgcg ccattcttcc acgggaggca acaggaccag cgtcaataga gcgtcatgca
     1921 gttcatcggc gtcgcgaact accggccagg attgctcgga tacttccgcg atcgccgccg
     1981 gatccagcgc tccaatgcca gtggcgtccg ccggcggcag accgcgtcga agctgtacgg
     2041 cgcgggtgcg gcgctcttcg agaggcgcgt catccagaaa tgcgtatggg ttcgcattca
     2101 gaatctcgtg cgagaacacc gacggctctg gcgtgtcgat cgctacggtt ttgagcgtgc
     2161 cctcctccat gcgttcgagg atcgcgatca gcccgttcag atccatggcc tcatgcaggc
     2221 agttatcgat ggtttcgttg accagcggat gatccggaat gcggatctcg cccaccaggt
     2281 tctccgcgca cgccgcctga tccgggaaca ccgccgccag caaatcgtcg gcgcgcatgc
     2341 gttgaatcgg aggcggcacc ttgcgcccgc ccataaagcg cggcaccgcc aacgcccgcg
     2401 aggcgttcca tttccagcgc gccccgaaca tgggagccgc cagcagcgcg tccgtcaaca
     2461 ctttggttac cgtatccggc cgcagataac tgaagataat ttccagcggg aaagagtgtt
     2521 gatcgctcaa cgagagcacc agtccgttgt cggtcgccgc cgcttgcaac tcgaagttga
     2581 acgcgcggca gaagcgcttg cgcagcgcga gtccccaagc ccggttgatg cgcgacccga
     2641 acggggcgtg cacgatcatc tgcataccgc cggcctcatc gaagaatcgt tccgctacca
     2701 ccgtgtcttc gctgggcacc gcacccagag agcgcgcgcc cgcgcggacg tactcgaccg
     2761 cttgctccgc accgaggcga tcgaggccgc attccgacat cagcatgtca atcgccgcag
     2821 ccacgtcgcc gccggcaatc tctacacgca gttctgacac cgcgttcgac aactcgcgcg
     2881 tgcgaccggg cgcctcgccg cgccagaagg gaatcgtcgg tgcagcgccg tgggcgtctt
     2941 ccaccctaac ccgcccggtc tcgacacgct tgatgcgcca tgacgtggtg cctagcagaa
     3001 acacatcgcc cgccagactc tccaccgcga aatcttcgtc gatggtgccg acgacggttc
     3061 cctccggctc ggccaccacc tgatactgcg cgctatccgg gatcgcgccg ccattggtga
     3121 tcgccatcag ccgcgccccg cgccgtccgc gcagccggtg attcacctgg tcgcgatgca
     3181 ggaacgcccc gcggcgcccg cgcgatgtgg ccatgccctc ggagagcatc tcgaccaccg
     3241 catcgaagtc gcgccgagtg agatcgcggt agggatcaac tccgcgcacg aacgcgaaca
     3301 gcgcgtcttc atcccaatcg tcacaagccg ccattgccac gatctgctgc gccagaatgt
     3361 ccaacggcgc gcgcggaatc tccagacggt ccagttcgcc ccgcttgatc gaacgaacca
     3421 ccgccgcgct ttcaatcaac tcatcgcgag tggtagcgaa gattctgcct ttgggcttcg
     3481 ctcccaccca atgccccgca cgcccgacgc gctgcaacgc tactgaaatc gaccgcggcg
     3541 agcccatctg gcacaccaaa tcaacggtac caatgtcgat ccccaattcg agcgacgccg
     3601 tcgccaccac cgcgcgcaac tcgccgcgct tgagacgctg ctccgcttcg agacgcaacc
     3661 gccgcgacag gctgccgtga tgcggcagta ccacgccttc gcccatccgt tcgccgagtg
     3721 cgtgagcaat gcgctccgcc tgacggcgcg tgtttacgaa aaccagcgtc gtgcgatgtt
     3781 ctccgatgag tgcggccgtc cggtcgtaaa tctcggacca catttcgttg gatgccacgg
     3841 cccccaattc atcgcgcggc acctcgacgg caatatccat atcgcgccgc cgccccactt
     3901 gaatgatgtg cgcgcgatcg ctgagaaatc gccccacctc ttcaatggga ctcaccgtcg
     3961 ccgacaaccc aatcttctgg acccgcccct ccgccagcgc ctccagccgt gcgatcgaca
     4021 gcgccagatg cgagccgcgc ttcgaccccg ccaccgcgtg aatttcatcc acgatcagcg
     4081 tgcgcaccga gcgcagcatc tcccgcgact tgcccgcggt gagcaggatg aacaacgatt
     4141 ccggggtcgt caccaagata tgcggcgctt ttctggtcat gagcgcccgc tcggccatcg
     4201 gcgtgtcgcc agtgcgtacc gcggctcgaa tctccggcag cggcgtgccc ttggcgcgcg
     4261 ctagttccgc gattccggcc agcgggatat ccaggttcac gcgcacatcg ttgctcaggg
     4321 ccttgagcgg cgacacatag aggatgcgcg tc
//
LOCUS       DASZMC010000594         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2992500,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000594 DASZMC010000000
VERSION     DASZMC010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2992500"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VG274_12095"
     CDS             complement(<1..698)
                     /locus_tag="VG274_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWE07446.1"
                     /translation="MSIEISKLANGLTVVSDPMPGLESAFVGVWVNAGGRNETRPVMG
                     VSHMLEHMAFKGTKRRSARDIAEEIEAVGGYLNAYTSREQTAFHARVLKADVPLGLDI
                     LADILTEPTFEQAELERERQVVLQELGQSRDTPDDIIFDHLQAVVYPDQPMGWPILGE
                     EETVSIFSQTQLRDYMRANYRAGSMVLVASGAVDHKQIVAMAEEKFASLPTGTGSTPL
                     TARYHGGDKRAGDDL"
     gene            complement(695..>1687)
                     /gene="thrC"
                     /locus_tag="VG274_12100"
     CDS             complement(695..>1687)
                     /gene="thrC"
                     /locus_tag="VG274_12100"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWE07447.1"
                     /translation="RAMVLAATSGDTGSAAIAALGGVGNIDVFVIHPKGRTSEVQRRQ
                     MTTSKHANVHNIAMDGNFDDAQSIVKQLFADDGFARAAHLTAVNSINFARIAAQSVYY
                     FTATAKLGKPANFVVPTGNFGDVFAGEAAMRMGLHANKLVVATNANDIMARSLNDGVY
                     ASGAAHATLRPSMDIQVASNFERALFEASGRNTAWLRDAMHTFMRDKSLKLPDNVRDA
                     LRARYLAARGDDEETLAAIAKLHHDTGRTIDPHTAVGLVAADKLKSQLSSPVVVLSTA
                     HPAKFPDAVVKATGQKPPLPPRLSGLYEGVEQMTVLPADQAAVRGFIRERLQAA"
BASE COUNT          288 a          504 c          550 g          345 t
ORIGIN      
        1 agatcgtcgc ccgcacgttt gtcgccgccg tgataccgcg cggtcagcgg cgtgctgccg
       61 gtgccggtcg gcagcgatgc gaatttctcc tctgccatcg cgacgatctg cttgtgatcg
      121 acggcgccgg atgccaccag caccatcgaa ccggcgcgat aattcgcgcg catgtaatcg
      181 cgcagctgcg tctgggagaa tatcgaaacg gtttcttctt cgccgaggat cggccagccc
      241 atcggctgat cgggatacac gactgcctgc agatgatcga agatgatatc gtccggcgta
      301 tcgcgcgatt ggccgagctc ctgcaacacg acctggcgtt cgcgttccaa ttccgcctgt
      361 tcgaaggtcg gctcggtgag aatgtcggcg aggatatcga gaccgagcgg cacgtcggct
      421 ttgagcacac gtgcgtggaa agcggtctgt tcccggctgg tataggcgtt gagatagccg
      481 ccgacggctt cgatctcttc ggcgatgtcg cgggcggagc ggcgtttggt gcctttgaac
      541 gccatgtgct ccagcatgtg cgaaacgccc atgacgggac gggtttcgtt gcggccgccg
      601 gcgttcaccc agacgccgac gaaggcgctt tcgagcccag gcattgggtc agaaacgacc
      661 gtcaagccat tggccaattt ggaaatttcg atgctcatgc tgcctgcaat ctttcccgga
      721 taaagccccg gaccgctgcc tgatctgcgg gcaggacggt catctgctcc accccctcat
      781 agaggccgga aagccgcggc ggcaaggggg gcttttggcc agtcgctttg accacggcgt
      841 ccgggaactt ggcagggtgc gcggtggaaa ggaccaccac cgggctgctg agttggctct
      901 tcaacttgtc tgcggcgacc aaaccgacag ccgtgtgcgg atcgatggtg cggccggtat
      961 cgtgatggag cttggcgatg gcggcgagcg tctcttcgtc gtctccgcgc gcggcgagat
     1021 agcgcgcgcg gagggcatcg cgaacgttgt cgggcagctt cagtgatttg tcgcgcatga
     1081 atgtgtgcat cgcgtcacgt agccacgccg tgttgcgccc gctcgcttcg aacagcgcgc
     1141 gttcgaagtt gctggcgacc tgaatgtcca tcgacggtct gagcgttgcg tgcgcggcac
     1201 cggacgcata gacaccatcg ttcagcgagc gcgccataat gtcgttggcg ttggtggcga
     1261 ccacgagttt gttcgcgtgc aaacccatcc gcatcgccgc ttcgcctgcg aacacgtcgc
     1321 cgaaattgcc ggttggcacg acgaagttag caggcttgcc gagcttcgca gttgccgtga
     1381 agtaataaac gctctgcgcc gcgatgcgtg cgaaattgat cgagttcacc gcggtcaaat
     1441 gcgccgcgcg cgcgaagcca tcgtcggcga agagttgctt cacgatgctc tgcgcgtcgt
     1501 cgaaattgcc gtccattgca atgttgtgga cgttggcgtg cttgctcgtc gtcatctggc
     1561 ggcgctgcac ctcactggtg cggcctttcg gatggatgac gaacacgtcg atattcccga
     1621 caccgccgag cgcggcgatg gctgcggagc ccgtgtcgcc ggatgttgcc gccaaaacca
     1681 tggcgcg
//
LOCUS       DASZMC010000595         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2443974,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000595 DASZMC010000000
VERSION     DASZMC010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443974"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VG274_12105"
     CDS             complement(<1..831)
                     /locus_tag="VG274_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE07448.1"
                     /translation="MNRAQRRSISKSKDCADVAGLAAFYQRSGRLDEAKQKYREAIAL
                     NPASLEAHNNLGLILQETGHAAEATAQFARAFKIAPANARVAFNFARALAAQNRYQES
                     ATLFREAISLDARESDAHFGFALCLAQLGQHHDAERSYLKGLEIDPSNWQARASLGIA
                     LVDQGKIVDAFAQAEILARAAGTATGFPHKSFGILLTRAGCPDGARECFETHLSRNPA
                     DKDEIAMLLAAVGGALPDRASDRQVERLYAARAAEWDQGATGPTGYRGHRLVVAAIDE
                     L"
     gene            complement(920..1177)
                     /locus_tag="VG274_12110"
     CDS             complement(920..1177)
                     /locus_tag="VG274_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07449.1"
                     /translation="MNLSFATVLFAAIVASLIAIPNPQQQPAPVIVAPVVVAKTDRLP
                     AQPAVSACAQQNWPNFDAACLRYTDGRQAVRGVRVAAGDQN"
     gene            complement(1276..>1672)
                     /locus_tag="VG274_12115"
     CDS             complement(1276..>1672)
                     /locus_tag="VG274_12115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE07450.1"
                     /translation="LVSEAFLARRIGNLDAFLLAFDQDARYDSPNYLWFRSRYARFVY
                     IDRIVVASSARKRSCARQLYHDLFGHALRAGHDRVFCEVNLSPPNPLSDAFHAALGFA
                     EVGTAEQGDRIVRYLARALSEDDRKPSPH"
BASE COUNT          318 a          503 c          542 g          309 t
ORIGIN      
        1 cagttcgtca atggccgcaa cgaccaaccg atggccgcga tagccggtcg gcccggttgc
       61 gccctgatcc cattccgccg cacgtgcggc gtacaggcgc tcgacctgcc ggtcggatgc
      121 gcgatctggc aacgcgccgc cgaccgcggc gagcagcatc gctatttcgt ccttgtcggc
      181 ggggttgcgc gaaaggtggg tttcaaaaca ttcccgggcc ccgtcgggac aaccggcccg
      241 cgtcagcagg ataccgaagc tcttgtgggg aaatcccgtt gcggttcccg cggcgcgcgc
      301 cagaatttcg gcctgcgcaa aagcgtccac aatttttccc tggtcgacca gcgcaatgcc
      361 gaggctcgcg cgggcctgcc aattcgacgg atcgatctcc agccccttca gatagctccg
      421 ctcggcgtcg tgatgttgtc caagctgcgc aagacaaagg gcgaagccaa aatgcgcgtc
      481 actctcacga gcatccagcg aaatagcttc ccgaaacagg gtagcggatt cctggtagcg
      541 gttttgcgct gccagcgccc gggcgaaatt gaaggcgacg cgcgcattcg cgggcgcgat
      601 cttgaaggcg cgggcaaact gcgccgtcgc ttccgccgcg tgaccggttt cctgcaggat
      661 caggccgagg ttgttgtgtg cttcgaggct cgcgggattg agcgcaatcg cctcgcggta
      721 tttctgcttg gcttcatcga gccggccgct gcgctggtag aacgcggcga ggcccgcaac
      781 atccgcgcaa tccttgctct tcgatatgct gcggcgctgg gcgcggttca tgaaaatctt
      841 cccaaagatg gcgcatctta gggagcatca tggttagcga acggttatcg ggaactcgat
      901 cgaccctatt ccgccgccgt cagttttgat cgcctgccgc gacgcgaacg ccgcgaacgg
      961 cctgtctgcc atcggtgtag cgcagacagg ccgcgtcgaa gttgggccaa ttctgttgcg
     1021 cgcaggcgga cactgccggc tgggccggga gccgatcggt ctttgcgaca acgaccggcg
     1081 cgacaatgac cggcgccggc tgttgttgtg ggttcggtat ggcgatcagg gatgcgacga
     1141 tcgccgcgaa caacacggtc gcaaaactca gattcatcag gccggctttc ctgcacgcgg
     1201 tggaaatttg ccggactgat agcgtgcgag gtcaatctcg caaccgaact cgattgttaa
     1261 gctgaagcgt caagcttaat gcggcgaggg ctttcgatcg tcttcggaaa gggcgcgcgc
     1321 gagatatcgg acgatgcggt caccttgctc agcggtgccg acttcggcaa atccgagtgc
     1381 cgcatggaac gcgtccgaga gcggattggg cggcgacaga ttgacttcgc agaaaacacg
     1441 atcgtgcccg gcacggagcg cgtgaccgaa caggtcatga tagagctgcc gcgcgcagct
     1501 gcgcttgcgc gccgatgagg ccaccacgat gcggtcgata tacacgaagc gcgcatagcg
     1561 gctgcggaac cagagataat tcgggctgtc gtagcgggcg tcctgatcga aggcgaggag
     1621 gaaagcgtcg agattgccga tccgcctggc caaaaacgct tcgcttacga ga
//
LOCUS       DASZMC010000596         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2993225,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000596 DASZMC010000000
VERSION     DASZMC010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2993225"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /gene="nusA"
                     /locus_tag="VG274_12120"
     CDS             complement(<1..1415)
                     /gene="nusA"
                     /locus_tag="VG274_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWE07451.1"
                     /translation="MAAIAANRTELLQIADAVARDKSIDKSVVLHAMEEAMQRAAKAR
                     YGSENEIRVEIDDKTGQTHVSRYLHVVELVENDKTEIALEDARVRNPAAQVGDIIAET
                     LPPVDFSRIAAQAAKQVIVQKVRDAERERQYEEYKDRIGEIIHGIVKRVEFGSVVVDL
                     GRAEGVVRRDEMIPRESFRTNDRIRAYIYDVRRETRGPQIFLSRSHPQFMVRLFAQEV
                     PEIYDGIIEIRAVARDPGSRAKIAVISKDSSIDPVGACVGMRGVRVQAVVQELQGERI
                     DIIPWSPESATFIVNALAPAEVTKVVLDEDTHRVEVVVPDEQLSLAIGRRGQNVRLAS
                     QLTGWQLDILTEQEESERRQKEFAERTQLFMESLDVDETVAQLLASEGFASVEDVAYV
                     SLNDLAAIEGFDEETAQELQNRALEFIDARNEKLDQKRRELGVEDEIVELGGISPPML
                     VAIGEAGIKSLEDLAGCATDDL"
     gene            complement(1432..1929)
                     /gene="rimP"
                     /locus_tag="VG274_12125"
     CDS             complement(1432..1929)
                     /gene="rimP"
                     /locus_tag="VG274_12125"
                     /inference="COORDINATES: protein motif:HMM:NF000932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWE07452.1"
                     /translation="MSAVAHLEPIISPAVEAAGYRLVRLRLMGGKHRTLQIMAERADG
                     QMTVEDCARLSRELSKFLEGEDAIEDDLVLEVSSPGIDRPLTRLMDFARWAGHEAKIE
                     LMAPDSSGRRRFKGLLLGLDGGNVVIGLEGARLAFPYGSIAEAKLVLTDRLVEEDLKA
                     RKPGA"
     gene            complement(2046..2804)
                     /gene="trmB"
                     /locus_tag="VG274_12130"
     CDS             complement(2046..2804)
                     /gene="trmB"
                     /locus_tag="VG274_12130"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HWE07453.1"
                     /translation="MDQSDRAERRRRLLFGRRKGPRLSAHQANLRATLLPQLRLPLRP
                     DIDPAALFASSVADVWLEIGFGAGEHLLWQAQHHPDVGILGVEPYEAGVAKLLSKVFS
                     LSAQGETSNPISNLRIHEGDARDVLETLPEACLARVFLLFPDPWPKTRHHKRRFIQMD
                     VLDALARAMKRDAEFRFASDDAGYVEWTLEHLLAHDSFVWMAERSQDWRRRPDDWPPT
                     RYEQKALQGVPVFLRFRRSERRRGLLQTDRPKRK"
     gene            complement(2806..>3237)
                     /locus_tag="VG274_12135"
     CDS             complement(2806..>3237)
                     /locus_tag="VG274_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HWE07454.1"
                     /translation="RKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNIVA
                     ADLADRCTIQISYAIGVSDPLSLYVDLHGTGRVNEARLQKLLPELVSLRPRAIRDRLG
                     LNRAIYARTAAYGHFGREPDSDGGFSWERTDELAPELTRTF"
BASE COUNT          579 a         1049 c          976 g          633 t
ORIGIN      
        1 aggtcgtctg tggcgcaacc tgccagatct tcgagcgatt tgatgcctgc ttcgccaatc
       61 gcgacgagca tcggggggct tattccgccg agttcgacga tttcgtcctc gacgccgagt
      121 tcgcggcgtt tctgatcgag cttctcgttg cgggcgtcga tgaattccag ggcgcggttc
      181 tgcagctctt gcgcggtttc ctcgtcaaaa ccctcgatgg cggcgagatc gttcaacgag
      241 acataggcaa cgtcttcgac gctcgcaaag ccctccgaag cgagaagttg cgcaacggtt
      301 tcgtccacat cgagcgattc catgaacagc tgggtacgct cggcaaattc cttctggcgc
      361 cgctccgatt cttcctgctc cgtcaggatg tcgagctgcc agccggttag ctgcgacgcc
      421 agcctcacgt tctgcccgcg ccttccaatc gcgagcgaga gctgttcgtc gggaacgacg
      481 acttccacgc gatgtgtatc ttcgtcgagc accaccttcg tcacttccgc gggcgcaagc
      541 gcattgacga tgaacgtcgc ggattcgggc gaccacggaa tgatgtcgat acgctcgccc
      601 tgcagttcct gcaccacggc ctgcacgcgc acgccgcgca ttcccacgca ggcgcccacg
      661 ggatcgatcg aggaatcctt cgaaatgacc gcaatcttcg cgcgcgagcc gggatcgcgg
      721 gccaccgcgc gaatctcgat gatgccgtcg tagatctcgg gcacctcctg cgcgaacagg
      781 cgcaccatga attgcgggtg gctgcgggag agaaatatct gcggcccccg cgtctcgcgc
      841 cgcacgtcgt agatataggc gcggatgcgg tcgttggtgc ggaaactctc gcgcggaatc
      901 atctcgtcgc gtcgcacgac tccttccgca cggccaaggt cgacgacgac ggagccaaac
      961 tccacgcgct tcacgatgcc gtggatgatc tctccaatgc ggtccttgta ctcctcgtac
     1021 tggcgctcgc gctcggcgtc gcgcaccttc tgcacgatca cctgctttgc ggcctgcgcg
     1081 gcaatccgcg agaaatcgac cggcggcaac gtctcggcga tgatgtcgcc cacctgcgcc
     1141 gccgggttcc gtacacgcgc gtcttcgagc gcaatctccg tcttgtcgtt ctcgaccaac
     1201 tccacgacgt ggagatagcg ggagacgtgc gtctggccgg tcttgtcgtc tatctcgacg
     1261 cgaatttcgt tctcgctgcc gtaacgcgct ttcgcggcgc gttgcatggc ctcctccatt
     1321 gcatgcagca ccacggactt gtcgatggac ttgtcgcgtg caaccgcgtc ggcgatttgc
     1381 aaaagttcgg tcctgttggc ggcaatcgcg gccatgatat gcttctcccg ttcaggcgcc
     1441 cggtttgcgc gccttcaagt cctcttcaac cagcctgtcg gtcagcacga gctttgcttc
     1501 ggcgatgctg ccgtacggaa atgcaagtcg cgcgccttcg agtccgatga cgacgttccc
     1561 gccgtccagg cccagcagca aacccttgaa acgccttcgc ccgctggaat cgggcgccat
     1621 cagttcgatt ttcgcttcgt gtccggccca gcgcgcgaaa tccatgaggc gcgtcagcgg
     1681 ccgatcgatc cccggcgatg acacctcaag cacaaggtcg tcttcgatcg catcctcacc
     1741 ttcgagaaac ttcgaaagct cacgcgacag ccgcgcgcaa tcctcgaccg tcatctgtcc
     1801 gtcggcgcgt tccgccatga tctgcaaggt cctgtgtttc ccacccatca gcctcagacg
     1861 cacgagccga tagccggctg cctcgacggc cggagagatg atgggctcca aatgcgctac
     1921 tgcgctcaag atcgcgcgtc tccttcgatc acaggaattc tgggcgccgg caaaggcaaa
     1981 acccgtcccc cgcggcggtc ttgcgactgc cacacaacag cttttctgtt gtcggggatg
     2041 ggggcctatt tacgctttgg ccggtccgtt tgcaagagcc cgcgacgcct ctcgcttcgc
     2101 cgaaatctca gaaatacggg cacgccttgg agtgcctttt gttcgtaacg ggtcggcggc
     2161 cagtcgtcgg ggcgccgacg ccaatcctgt gaccgttccg ccatccacac gaacgagtcg
     2221 tgcgcgagca aatgttcgag agtccattcg acatagccgg catcgtcgct tgcaaaacga
     2281 aactcggcgt cccgtttcat cgcgcgagca agggcgtcga gcacatccat ctggatgaag
     2341 cgccgcttgt ggtgccgggt tttcggccag ggatcgggaa acagaaggaa cactctggcc
     2401 aggcacgctt cgggcagcgt ctccaaaacg tcgcgcgcgt cgccttcgtg gatgcgcagg
     2461 ttggaaatcg gattggaggt ttctccctgg gcagaaagcg aaaagacctt cgacagaagt
     2521 ttggcgacac ctgcttcgta aggttccacg ccaaggatgc cgacatcggg atgatgttgt
     2581 gcctgccaaa gcagatgctc gccggcaccg aagccgatct cgagccagac atcggcaacg
     2641 ctcgaagcga agagcgctgc cggatcgata tcgggcctga ggggcagccg cagctgcggc
     2701 agcagcgtgg cacgcagatt ggcctgatgc gcagagagcc tgggaccctt gcggcggccg
     2761 aagagcaaac gccgcctgcg ctcggcccgg tcggattgat ccacgtcaga acgtgcgggt
     2821 gagctccggc gccagctcgt ccgtgcgctc ccacgagaag ccgccatcgc tgtcgggctc
     2881 gcggccgaaa tggccatagg cggccgtacg cgcgtagatc gcgcgattga gtcccaatct
     2941 gtcgcgaatc gcgcgaggcc tcaggcttac cagctccggc agcagtttct gcaacctggc
     3001 ctcgttcacg cgcccggtgc cgtgcagatc gacataaagc gacagcggat cggaaacgcc
     3061 gatcgcatag gagatctgga tcgtgcagcg atcggcgagg tcggcagcga cgatgttttt
     3121 cgccagatag cgcgcggcgt aggctgcgga acggtcgacc ttggtcgggt ctttgcccga
     3181 aaaagcgccg ccgccatggg gcgccgcgcc gccataggtg tcgacgatga tcttccg
//
LOCUS       DASZMC010000597         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4641557,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000597 DASZMC010000000
VERSION     DASZMC010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4641557"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..1990)
                     /locus_tag="VG274_12140"
     CDS             complement(365..1990)
                     /locus_tag="VG274_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWE07455.1"
                     /translation="MEVRYPPSAVQAVAQAVGADDVARAAALADAALANGLNHPILFI
                     ARALRFERQARDEDALAAYQQARALAPKDPRLLNAIGLCLLRLHRYREALAAFDRAVH
                     VKPAASATHQNRGLALGMAGRAEDAERAHTRAVRLDPRNVEALTSLASIAAHKGEAGT
                     ARDYAERALEIDSRNLAARASLALAEVSLGQFADAESRLRLLVEEPGLTGHARAVAFA
                     LLGDALDGQDRCPDAFAAYTAANTERRKLHQPRFRGMPGAIDTLEHLAASFGATSDDQ
                     WRAPGRDEPAQGAVRHIFLLGFFRSGTTLLGQVLDSHPGIVTLDERDFLADMAERTLN
                     GADGFARLSASSEDELAIHRKTYWQRVRDQGLRVEGKVFVDKHPFHTIKLPLIFKLFP
                     HAKVLFALRDPRDVVLSCFRRQLDVDQLKFEFLTLEGAARLYDCVMRFGELCRRKLPL
                     AILDHRYEDLVGAFDDRTRTVCDFIGVPWSDSMHDFAAAAQSLGAQKPSARQVRRGLY
                     SAGAGRWRQYRSQLEPVLPLLEPWIERFGYSGE"
     gene            complement(2010..2516)
                     /locus_tag="VG274_12145"
     CDS             complement(2010..2516)
                     /locus_tag="VG274_12145"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWE07456.1"
                     /translation="MLNLIPPVWAAIYLGLAGAASAIFPWRTTVDLRIAWFGVVLIAA
                     GAAFAVSAALLFRAEETEISPTSQTNRRLVIRGPYRLTRNPMYLGLVLASLGIAFCVG
                     SLPMFAAPLLNFATTNQVHIPFEEARMRRQFGQAYDHPRNAPLGLISGVGSPGLPWFL
                     AVTQCGGG"
     gene            2679..3035
                     /locus_tag="VG274_12150"
     CDS             2679..3035
                     /locus_tag="VG274_12150"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWE07457.1"
                     /translation="MSTRELANDLVALCKQGKFADAGEKHWADNVLSVEPMGDNAELR
                     GKAAVRGKGEWWANNHEVHSAVVEGPYVHGDQFVVRFKMDMTIKASGERRSMDEVGLY
                     TTRNGKIVEERFFYGG"
     gene            3064..>3309
                     /locus_tag="VG274_12155"
     CDS             3064..>3309
                     /locus_tag="VG274_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07458.1"
                     /translation="MKINPYLNFNGNCAEAFQFYAKVLRGKDLHIMSFRDSPMGDQVA
                     ANEKDLVVHARFAAGDNLIMGSDAPGGRYNKPQGYAVN"
BASE COUNT          639 a         1018 c         1105 g          547 t
ORIGIN      
        1 gatggcggcc agaagcggca tcagccagga attcggcctc agattgcgca acgccaggcg
       61 caattgctcg agcgccagtc tcgcgctccg ctctgggtcg gctttcagcc gcgcggccgt
      121 atccggcatt ccctgcaatc ccgatggctg ctggcagctt cgccatcgtg ccccgacacg
      181 gttaagatcg tcctccgtta tttggtgagt ttcaggttaa cggcccggtg ggcgggtgcc
      241 tggccctctg gtcggagcga attttcccga taccaaaaag cgatctccgc aatgctcgcc
      301 gtgggcaatg ccaactccgc gtcgtttcac acgagccacg caaccactgg ggggctcggc
      361 gtgctcattc cccggaatag ccgaaacgct cgatccaggg ctcaagaagc ggcaggactg
      421 gttcgagctg gctgcgatac tgacgccagc ggcccgcgcc tgcggaatag agtccgcggc
      481 gaacctgtct ggcgcttggc ttttgcgcac cgagcgactg cgcggcagcg gcaaaatcgt
      541 gcatcgagtc gctccacggc acgccaatga agtcgcagac ggtgcgcgtg cgatcgtcga
      601 aggcgccaac caggtcctcg tagcggtggt cgagaatggc gagcggaagc ttgcggcggc
      661 acaattcgcc gaaccgcatc acgcagtcat aaaggcgcgc ggcgccttcc agtgtcagga
      721 actcgaattt cagctggtcg acgtcgagct ggcgccggaa gcagctcagc acgacatcgc
      781 gaggatcgcg gagggcaaac agcactttcg cgtgcgggaa caatttgaat atcaatggca
      841 gcttgatcgt gtggaaggga tgtttgtcca cgaagacctt gccttcgacc ctgagaccct
      901 ggtcgcgcac ccgctgccaa taggtcttgc ggtggattgc cagttcgtcc tcgctggatg
      961 ccgaaaggcg ggcaaacccg tcggcgccat tcagggtgcg ctccgccata tccgccagga
     1021 agtcgcgctc gtcgagcgtg acgatgccgg gatgactgtc gagcacctgc cccagaagtg
     1081 tggtgccgga gcggaagaat ccgagcagaa atatgtggcg gacagcacct tgcgcgggtt
     1141 cgtcgcggcc gggggcgcgc cattgatcat cactcgtggc gccgaacgaa gcggcaaggt
     1201 gttccagagt gtcgatagct cccggcattc cccggaaccg cggctggtga agcttccggc
     1261 gttccgtatt cgcggcggta taggcggcga acgcatccgg gcaccggtct tgtccatcga
     1321 gcgcgtcgcc aagaagcgca aacgcgacgg cccgtgcgtg gccggtgagg cccggttcct
     1381 caacgaggag gcgaaggcgg ctttcggcat ctgcgaattg gcccagtgaa acttcggcaa
     1441 gcgccagcga agcgcgtgcg gccagattgc gcgaatcgat ttcaagcgcg cgctccgcat
     1501 aatcgcgcgc cgtcccggcc tctcccttgt gggcggcaat ggaggcgagg cttgtcagcg
     1561 cctccacatt tcgcggatcg agccggacag cgcgggtgtg cgcgcgttcc gcgtcttcgg
     1621 cccggccggc catgccgagc gccagccccc tgttctgatg ggtcgccgag gccgccggct
     1681 tgacgtgaac cgccctgtcg aatgccgcga gagcctcgcg gtatctgtgg agccgcaaca
     1741 ggcaaaggcc aatcgcgttg agaagccttg gatccttcgg cgccagagcg cgtgcctgct
     1801 ggtaggcagc gagtgcgtct tcgtcgcgag cctggcgctc gaaccgaagc gcgcgcgcga
     1861 taaacaggat gggatggttg agtccgttgg ccagcgccgc atcggccagt gccgctgcgc
     1921 gcgcgacgtc gtcggcgccg acagcctgcg ccaccgcctg gacggcggac ggcggatatc
     1981 taacctccaa agttggccct gactgcaact cagccgccgc cacattgtgt cacggcgaga
     2041 aaccaaggca accctggcga tcccacaccg gaaatcaaac ccagcggcgc gtttcgcggg
     2101 tggtcatagg cttggccgaa ttgccggcgc atcctggctt cctcgaacgg gatgtgaacc
     2161 tggttcgtgg tcgcaaaatt gagcagcggc gccgcgaaca tgggaagcga tccgacgcag
     2221 aaggcgatgc cgagcgaggc cagcaccagg ccgagataca tcgggttgcg cgtcaggcgg
     2281 taggggccgc ggatgacgag cctgcggttg gtctgcgacg tgggactgat ttccgtctcc
     2341 tcggcccgga acagcagcgc cgccgaaacc gcgaatgcgg cgccggcggc gatgagaacg
     2401 acgccgaacc acgcaatccg cagatcgacg gtcgttcgcc aggggaagat cgcgctcgcg
     2461 gcgccggcga gcccgagata gatcgcggcc cagacgggcg gtatcaaatt cagcattcgc
     2521 tggccgcgca ccggtgaaac ccgtcacgat ggcgcgacgg gacgcgaagc gtggcaaccg
     2581 ttgccccggc atttgcgttt cgcagcggag cacgacctgt cgcatttcgc cgctatgctg
     2641 tattgcggcg cgcgaagggg ccgagcaggg agaatctcat gagcacgcgg gaactcgcca
     2701 acgacctcgt ggccttgtgc aagcagggca aattcgcgga tgccggcgaa aaacactggg
     2761 ccgacaatgt gctgagcgtc gagccgatgg gcgacaacgc cgaattgcgc ggcaaagccg
     2821 ccgttcgcgg caagggcgaa tggtgggcga acaatcacga agtccacagc gccgtggtcg
     2881 aaggccccta tgtgcacggc gaccagttcg tcgtgcgctt caagatggac atgaccatca
     2941 aggcgagcgg cgagcggcgc agcatggatg aagtcggcct ctacacgacc cggaatggaa
     3001 aaatcgtgga agagcggttc ttctacggcg gatgagcggg gcgaaagcca ggaggaaaag
     3061 acgatgaaga tcaatcccta tctcaatttc aacggcaatt gcgccgaagc gtttcagttc
     3121 tatgccaagg ttctgcgcgg caaggacctg cacatcatga gctttcgcga ttcgccgatg
     3181 ggcgatcagg tcgccgccaa cgagaaggat ctggtggtcc acgcgcggtt cgctgccggc
     3241 gacaatctga tcatgggctc ggatgcaccg ggcggccgct acaacaagcc gcaaggctac
     3301 gccgtgaac
//
LOCUS       DASZMC010000598         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5188948,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000598 DASZMC010000000
VERSION     DASZMC010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5188948"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..544
                     /locus_tag="VG274_12160"
     CDS             380..544
                     /locus_tag="VG274_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07459.1"
                     /translation="MGDLLGNAVRFGVADDDVLVAGEGIEIMLSLRCVLPTLPLASIG
                     DTWPECPPNR"
     gene            834..2552
                     /locus_tag="VG274_12165"
     CDS             834..2552
                     /locus_tag="VG274_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07460.1"
                     /translation="MSLTDCQRFFEQPATARQRQYEALRAYFLEEVPSAEAARSFGYA
                     PGAFRVLCHAFRHGRLPDFFATGHAVRREQPRKNRAHEQIVALRKRNYSVYEISQALK
                     EQGTPLSATAVREVLAEEGFAPLPRRLDEERPERVGPSAEAVANVRDFVLSPREFTTR
                     VGGLFLFVHDLVRLDGDALALGATLPGSAMIPAGHALRAALALKLWSIERKSHVMALV
                     ADEGLALFCGFNAMPKKSFLSEYSSRIAPRKVSRLLGLWHDQVTGEALVTGESINLDF
                     HSVPYFGEHPLVESHYLSMRSRRQPSILTFLAQDADSQVFCYSNADIRKGEEAEEVFR
                     FIAFWKRQYGSSPRHLVFDSKLTTYDRLDRLDAAGITFITLRRRTPSLLAEIDGLPLS
                     AWRTAKLDISSRKYRTPRYFEQPARPHKRDFRQFFITDLGHDEPTILLTNDASSTAKA
                     IITRYAKRMLIENALSDAVRFFHINALSSSVGFKVDFDMALLVIASGLYRLMARRMRG
                     YDDAQARQIFRDLIDMPADVAITEREITVRFHRRAHLPIVLASGLIDKPVAVPWWNGR
                     ALRLVQ"
     gene            complement(2535..3329)
                     /locus_tag="VG274_12170"
     CDS             complement(2535..3329)
                     /locus_tag="VG274_12170"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWE07461.1"
                     /translation="MLRRFRRVPPFPEAIRPPGRSPMKTFRGTYTVMITPFTPTGDVD
                     VAALRAFVDWQIAEGIHGLIPLGSTGEFLSLDDDEKALVAEVVITQAAGRVPVLIGTG
                     AEDTREVVRLSRRAEKLGADGVMIIPPFYSTPTDDELVHHYKTVADAISLPIMVYNNP
                     ATANVDLKPPLVARLAEIDNCLYIKESTLEVTRVRDIIRLCGDRMTVFGGILGFESFV
                     EGAQGWVAVASNVAPHAMARIFSLAWISTARNEPNISVLSTLLDQA"
BASE COUNT          620 a         1142 c          981 g          659 t
ORIGIN      
        1 tcctacgacc taagtcgctc ccaagccccc gtgcggctgc gtaccgagaa tcgtgaacag
       61 ctgaagaccc ttgcttgtgc ggcgctcgcc acctcaatgg ggctcgaagc gctcttggca
      121 agcttccaac tacggtatcc aaccgcgcgt tttccgatag ctacttgcaa acgaacgttc
      181 aagagccccg tgcccgatct gagctcccag tcgttctgga atccactcac actattcgtc
      241 tccgccgcca tgtcatgatt ttcatcggcg cggtcattcc gtgcgcgctc gatcgggctg
      301 aacaagcgta gttcccggac ggaatagaca acaccatgtt cgcataggca aggccccgat
      361 cgaaaaacca cggcgtgcga tgggcgacct gctcggcaac gcagttcgct tcggcgtggc
      421 agatgacgac gtgctcgtcg ccggcgaagg catcgagatc atgttgtcat tacgctgtgt
      481 gcttccgacc ttgccattgg cctccatcgg cgacacctgg cctgaatgcc cgccgaatcg
      541 ctagcctgga tttccacggc aaggaatccc cctaacgcca gcgtcgcgta agcgactcat
      601 tggactcagg atttcgcctt ccgatgttat ccgagatagt gcgctgataa gccgattgcg
      661 cggtgtcacc tgctgtggcg gcgcagtctg gttctgatcg cgcgattgag gccgtgttcg
      721 cacaaagcac tgcagaatgc agctttctgc gttctggcgg catttgtggt tcaattcgca
      781 cttgacacca gatgcagtat gtatgaccgt gcactgtgtc tatcagcgca ccattgagcc
      841 taacggactg tcagcgcttc ttcgagcagc ccgcaaccgc tcgtcagcgc cagtatgagg
      901 ctctgcgagc ctacttcctc gaggaagtgc cgtccgccga ggccgcacgc agtttcgggt
      961 atgcgccggg ggcgttccgt gtgctgtgtc acgcctttcg ccatggtcgg ttgccggact
     1021 tcttcgcaac cgggcatgcc gttcggcgcg aacagccgag gaagaaccgc gcgcatgagc
     1081 agatcgtggc gctgcgcaag cgcaactact cggtctacga gatcagtcag gcgttgaagg
     1141 agcagggaac accgctgagc gccactgcgg tgcgagaggt cctcgccgag gaaggctttg
     1201 cgcccctgcc gcggcggctc gacgaggagc ggcccgagcg ggtcggtcct agcgcggagg
     1261 cggtcgccaa tgttcgcgac tttgtgctgt caccgcgtga gttcaccacc cgggtcggcg
     1321 gcttgttcct gtttgtccac gatctggtgc gtctcgatgg cgatgcactg gcgctcggcg
     1381 ccacgctgcc gggctccgcc atgatcccgg ccggccatgc gttgcgcgcc gcgctcgcgc
     1441 tgaagctatg gtcaatcgag cgcaagagcc acgtcatggc gctcgtcgcc gacgagggct
     1501 tggccctctt ttgcggcttc aacgccatgc ccaaaaagag cttcctgtcg gaatattcct
     1561 cgcgcatcgc cccgcggaag gtctcccgcc tgctcgggct gtggcacgac caggtgaccg
     1621 gagaggccct cgtgacgggc gagtccatca acctcgactt ccattccgtc ccctacttcg
     1681 gcgagcaccc gctggtcgaa tcccactatc tatcgatgcg cagccgccgc cagccgagca
     1741 tcctcacctt cctggcccag gacgccgaca gccaggtgtt ctgctattcc aacgccgaca
     1801 tccgcaaggg cgaggaggcc gaggaggtct ttcgcttcat cgcgttctgg aaacgccagt
     1861 acggcagctc gccgcgccac ctcgtcttcg actccaagct caccacctat gaccgcctcg
     1921 atcgcctcga tgccgccggg atcaccttca tcacgctgcg ccggcgcaca ccgagccttc
     1981 tcgccgaaat cgacggccta ccgctatcgg catggcgtac cgccaaactc gatatctcca
     2041 gccgcaagta tcgcaccccg cgctacttcg aacagccggc tcggccgcac aagcgcgact
     2101 tccgccagtt cttcatcacc gatctcggcc acgacgagcc caccattctg ctcaccaacg
     2161 acgcctcgtc caccgcaaag gcgatcatca cccgctatgc caagcgcatg ctcatcgaga
     2221 acgcgctgtc cgacgcggtg cgcttcttcc acatcaacgc cctctcatca tcggtcggct
     2281 tcaaggtcga cttcgacatg gcgctcctcg tcatcgcctc aggcctctat cgcctcatgg
     2341 cccgccgcat gcgcggatac gatgacgctc aagcacgcca gatcttccgt gacctcatcg
     2401 acatgccggc cgacgtcgcc atcacggaac gagagatcac ggtgcgcttc caccgccgcg
     2461 cccacctccc catcgttctc gcctcgggcc tcatcgacaa acccgtagcc gtcccgtggt
     2521 ggaacggacg cgccctacgc ctggtccaat agcgtcgaaa gcaccgaaat gttaggctca
     2581 ttacgtgccg tggaaatcca ggctaggctg aagatgcgcg ccatggcgtg gggcgcgacg
     2641 ttggaggcaa ccgccaccca gccctgcgcg ccttcgacga acgattcaaa cccgaggatc
     2701 ccgccgaaca cggtcatgcg atcgccgcag agacggatga tgtcgcgcac gcgcgtcacc
     2761 tcgagcgtcg actccttgat gtagaggcag ttgtcgatct cggcgaggcg cgccaccagc
     2821 ggcggcttga gatcgacatt ggcggtcgcg ggattgttgt agaccatgat cggcagcgag
     2881 atcgcgtcgg cgaccgtctt gtaatgatgg acgagctcgt catcggtcgg cgtcgagtag
     2941 aacggtggga tgatcatcac gccgtccgcg cccagttttt cggcacggcg actgaggcga
     3001 acgacttcgc gcgtgtcttc cgcgccggtc ccgatcaata caggcacccg ccccgcggct
     3061 tgagtgatga cgacctccgc caccagcgcc ttctcgtcat cgtcgagcga caggaattcg
     3121 ccggtcgagc cgagcgggat cagcccgtga ataccttccg cgatctgcca atcgacgaag
     3181 gcacgcaacg ccgcgacatc cacgtcgcca gtcggcgtga acggggtgat catgaccgtg
     3241 taggtgccgc gaaaggtttt catcggggac ctgccgggtg gtcgaatggc ttcagggaat
     3301 ggcggcaccc tgcggaatct ccgcagcatt tatgcaatcg attgcgtaac tggctctgca
     3361 ttgagcaaca ccatccattt tttgggcttg atacacgcgg aa
//
LOCUS       DASZMC010000599         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3278240,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000599 DASZMC010000000
VERSION     DASZMC010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3278240"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VG274_12175"
     CDS             complement(<1..336)
                     /locus_tag="VG274_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101494.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWE07462.1"
                     /translation="MAQSSGSVPPRPVMLVILDGFGWREATEDNAVRLARTPTFTHLW
                     DSCPHAFLHTSGRDVGLPDGQMGNSEVGHMNIGAGRVVRQELVRIGDAVHEGSLAKAA
                     PLTDFIGKLR"
     gene            complement(455..1342)
                     /locus_tag="VG274_12180"
     CDS             complement(455..1342)
                     /locus_tag="VG274_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HWE07463.1"
                     /translation="MGSAFGNTLRFTNLDEVLRWALQAAIDHGQASSPRGMPTRELLA
                     HGFTLERPRQRRIMTPERRWSFPLALGEFCWHASGANDLSSIAYYAPRWREFSDDRQR
                     IDGSCYGTRIFGAMDGGPSQWDRLLKLLIQDPASRRAVLDLQSFGPALTNDARDVSCT
                     LGIQFFARGQRLHAAVNMRSNDIIWGLPYDVFLFTMLQELLACTLQLDLGCYFHFVGS
                     LHLYEHHIPLATRILSAPTPQECEMPRMEKPEQLSEFLQCESAIRTGEPYDMSVLCTY
                     WRDLAEVLRAYAARRRPVT"
     gene            complement(1342..2163)
                     /locus_tag="VG274_12185"
     CDS             complement(1342..2163)
                     /locus_tag="VG274_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07464.1"
                     /translation="MLTGNEIREKGLIVGAMEAGFRASSYDVHIESLIDSNGLVLESY
                     ALPAQGIVQAVSRERVVLPGDITGLAMVKTGLCNEGVLALNIGVIDPGWEGKISSFLV
                     NFSNHERLLIAGETFLRLTFQQLGAETSSRNPVSDEAYVADRRRQAVGKFGTTFLNLT
                     EATKAATEESFRQWRTSIFAYATPAALLLALLTFFLNFGSLYLIRTWLEPSATVEAEL
                     ARDTLSKNEAGLVQENRALANRVQALEERIGESTVARPNAASPQPTRIPPPGPPK"
     gene            complement(2259..2468)
                     /locus_tag="VG274_12190"
                     /pseudo
     CDS             complement(2259..2468)
                     /locus_tag="VG274_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101494.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
     gene            2621..3352
                     /gene="gloB"
                     /locus_tag="VG274_12195"
     CDS             2621..3352
                     /gene="gloB"
                     /locus_tag="VG274_12195"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HWE07465.1"
                     /translation="MAITAQPVPILSDNYAWLLRDSATGAVAIVDPAEPKPVIDALEK
                     AGGRLDLILLTHHHADHIAGADEVRAHFGAKMVGAAADAHRLPKLDIAVKEGDAVKFG
                     AAEARVIDTPGHTRGQINFFFPDGEILLSGDTLFSLGCGRLIEGTAEEMFASLRKLAA
                     LPGGTKVCCGHEYTESNARFALSVDGGNAALVARAAQAKQQRAAGQPTLPSTMADELA
                     ANPFLRAPDVASFAKLRSGKDSFRG"
BASE COUNT          668 a         1064 c         1119 g          652 t
ORIGIN      
        1 gcgaagcttg ccgatgaagt cggtcagggg cgcggccttt gcgaggctgc cctcgtgaac
       61 ggcgtcaccg atccgcacga gctcctggcg caccacgcga ccggcgccga tgttcatgtg
      121 gccgacttcc gagttaccca tctgcccgtc gggcaggccg acgtcgcggc cggaggtatg
      181 caggaaggcg tgtgggcagg agtcccacag atgcgtgaag gttggcgtgc gcgcgagacg
      241 gacggcgttg tcctcggttg cctcgcgcca gccgaagccg tccaggatca ccagcattac
      301 cgggcggggc ggcacggagc ctgatgattg tgccatgggc ccgattcctt cctgcggcaa
      361 agcgcctcgg agttagcctc cgtggcaaag cggcacaagg gcagtaaccg aaggcgcgag
      421 tgttcgcccc tgaattcaag cctctcaagc tggatcatgt caccggtcgc cggcgggcag
      481 cataggcccg caacacctcg gcgaggtcgc gccaataagt gcaaaggacg gacatatcgt
      541 agggttcacc agttctgatg gccgactcgc actggagaaa ttccgatagc tgttccggtt
      601 tttccatgcg tggcatctca cattcttgcg gcgttggcgc agacaggata cgcgtcgcca
      661 agggaatatg gtgctcgtat agatgaagcg atcctacgaa gtgaaagtag cagcccaggt
      721 cgagttgaag cgtgcatgca agcagttctt gcagcattgt gaaaagaaac acgtcatagg
      781 gcaggcccca gattatatcg ttgctacgca tgttgacggc ggcatgaagc ctctggcctc
      841 gtgcaaaaaa ttgtatgccg agcgtgcaag agacatccct cgcgtcattt gtaagcgctg
      901 gaccgaacga ctgcagatcc aagaccgcgc ggcgactggc aggatcctgg atcaacagct
      961 tcagcagacg gtcccactga ctcggtccgc cgtccatagc tccgaaaatc cgggttccgt
     1021 agcaacttcc gtcaatgcgt tggcgatcgt cggagaattc tctccagcga ggggcataat
     1081 aggcgatcga tgaaaggtcg ttcgcgccgg aggcgtgcca acaaaactca ccgagtgcca
     1141 ggggaaaact ccaacgccgc tcgggtgtca taatgcgacg ctggcgcggg cgttcgagcg
     1201 tgaagccgtg agcaagcagt tcacgggtgg gcatgccgcg gggcgaactc gcttgaccgt
     1261 gatcaatcgc agcctgcaac gcccagcgta gcacctcgtc aaggttggtg aatctcagag
     1321 tattgccgaa tgccgatccc atcattttgg cggccctggg ggaggaatcc gcgtgggttg
     1381 cggagaagca gcattcggcc gggcgacagt tgattcgcca atccgctcct ccagagcctg
     1441 cacccgatta gcgagggccc gattttcctg gaccaatcca gcctcgttct tgcttagcgt
     1501 gtcgcgagcg agttcggcct cgacagtcgc gctgggttcc aaccatgtac gtatcagata
     1561 taggcttccg aagttcagga agaacgtgag cagcgccagc agaagtgcgg ccggagtcgc
     1621 atatgcgaat atgctggtgc gccattgtcg gaacgactcc tcggtggcag ccttcgtcgc
     1681 ttccgtgagg ttcagaaatg tggttccaaa ctttcctaca gcttgccgcc gtctgtcagc
     1741 gacgtatgcc tcgtccgaga cgggattccg actggacgtt tcggcgccta gctgctggaa
     1801 cgtcaacctg agaaaggtct cacccgcgat gagcagccgc tcgtggttgc tgaagttgac
     1861 gagaaagctt gaaatctttc cctcccagcc tggatcgatg actccaatgt tgagtgccag
     1921 cacgccctca ttgcagagac cggtcttgac catggccagc ccggtgatat cgcccggcag
     1981 gacgacgcgc tcgcgcgaca ctgcctgcac gatgccttgc gccgggagag cataggattc
     2041 cagaaccaac ccgttggagt cgatcagaga ttcaatatgc acgtcgtaac ttgatgcgcg
     2101 gaacccagct tccatcgcac caacgatcaa gcctttctcc ctgatttcgt tgccggtcag
     2161 catctcggat tactgctccg cgatacgtac ggcacgccag gacggcgcga tcgtgctatg
     2221 ggggaaatgg gaaagatgcc ggcccgtgat ttccgtggcc cggcgccgat gttcatgtgg
     2281 ccgacttcgg agttgcccat ctgcccgtcg ggcaggccga cgtcgaggcc ggaggtatgc
     2341 gggcaggacg cccacgggcg cgtgaaggtt ggcgcgcgcg cgaggcggac ggcattgtct
     2401 tcggtcgcct cgcgccagcc gaagccgtcc aggattacca gcattaccgg gcgaatcggc
     2461 tgcgccatgg gtcgggtcct tgctgtggct gcaacagtta gcgttggtgt tggggcggaa
     2521 caagggcggt tttcggaggg ggccggctgc atcggtctgg gtaccgctgc ccgcccgccc
     2581 ggcggcttta ctcggccgcg cgcagtggta aactgccggc atggcaatca cagcacagcc
     2641 cgtccccatc ctctcggaca actacgcctg gctgctgcgc gacagcgcga ccggcgccgt
     2701 ggccatcgtc gatccggccg aaccaaaacc ggtgatcgac gcgctggaga aggcgggcgg
     2761 ccggctcgac ctgatcctgc tgacccacca ccacgccgat cacattgccg gcgccgacga
     2821 ggtccgcgcg catttcggtg cgaagatggt cggcgcggcg gcggacgcgc atcggctgcc
     2881 gaagctcgat atcgcggtga aggaaggtga cgcggtgaag ttcggtgccg ccgaggcgcg
     2941 ggtgatcgac acgcccggcc acacccgtgg ccagatcaat ttcttctttc ccgacggcga
     3001 aatcctgctg tccggcgata cgctgttcag cctgggctgc ggccggctga tcgaaggcac
     3061 ggctgaggag atgttcgcct cactgcgcaa gcttgccgcg ttgccgggcg gcacaaaggt
     3121 ctgctgcggc cacgaatata ccgagagcaa cgcacggttc gcgctcagcg tcgatggcgg
     3181 caatgccgcg ctggttgcgc gcgcggcgca ggcgaagcag cagcgcgcgg cgggccagcc
     3241 gacgctgcct tcaacaatgg ccgacgaact ggccgccaat ccgttcctgc gcgcgccgga
     3301 cgtcgccagc ttcgcgaagc tgcgctccgg caaggacagc ttccgaggct aatccgccgt
     3361 ggagttgttc aactctgcgg caaatccgtg tgcgcgcaag gtgctggcct gccaaccagg
     3421 cttctgaagt cgagcccgcg cttataatgt cgccttcagc attagcctct agagcacttc
     3481 caccgtgacc ggaatctgtc att
//
LOCUS       DASZMC010000600         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2996603,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000600 DASZMC010000000
VERSION     DASZMC010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2996603"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VG274_12200"
     CDS             complement(<1..357)
                     /locus_tag="VG274_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWE07466.1"
                     /translation="MPQLTMRDALNQALHQEMARDERVIVLGEDVSGGAGGTSGQREA
                     SGGIFGVTKGLLPRFGAGRVLDTPISESAIAGAAAGAALAGLRPVAEIMFADFIGVCL
                     DQVYNQIAKFRYMFGGK"
     gene            complement(376..1350)
                     /locus_tag="VG274_12205"
     CDS             complement(376..1350)
                     /locus_tag="VG274_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWE07467.1"
                     /translation="MNRNPSELLLPAAYRQMKTIREFEERLHVEIATGEIPGFTHLYA
                     GQEAVAVGVCSRLGDEDYIVSTHRGHGHCIAKGCDVVGMMKEIYGRKEGLCGGKGGSM
                     HIADFAKGMLGANAIVGGGPPIAVGAAIACKLRGNRSVSVAFGGDGSANQGTVFEAMN
                     MAVVLKVPAIFVMENNGYSEHTGAGYAVGSGDLASRSHAFGMPAERVDGSDFHAVYEA
                     MGRAVEHAESGGGPSTIEAMTTRFYGHFEGDPQNYRAKDEVSRHRATMDCLKRFRERT
                     RADATVSSAVLDAIDAEVLELVDRAVAEAKLAAPPGEADLYSDVYIQY"
     gene            complement(1347..>2723)
                     /locus_tag="VG274_12210"
     CDS             complement(1347..>2723)
                     /locus_tag="VG274_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWE07468.1"
                     /translation="LLRSTAPRYAPVSRVKRGADGRTADTPGTPHEKADDPDLGTASI
                     DPLRYTSRAFMRLEWERLWTRVWLVAGRSLDIAKPGDYLVTEIGPESIIVIRQADGNV
                     RAFYNVCQHRGNRLRSCGHGSTGPTLALKCLYHHWEYNLDGSYRRIPDVDTFPQGTPP
                     YGLSEVRCEEWGSFVWFSLDPQAAPLADFLSPIPLHLNPYHFERMVQTRDITVEWDCN
                     WKTSVDAFNESYHVQGIHPQLLWHLHDLDIQIDCYERHSRYLIPFGTVSPRVRKPPGI
                     PESIKTIMKSAGMDPADYDEPVANIRRDVQRQKRAVGAQQGRDYSELNDDQLTDDYHY
                     LIFPNLSLNLHADDFWIFRQRPHASDPDKMYYDIWTYELVPEGKTWPERAKHQSFRHG
                     DKSIGLVLDQDAANLPNVQAGMHSRGFKGLWLGRQELRIRHFHQVIDEYLFPAGADRE
                     SWTRGSDR"
BASE COUNT          472 a          922 c          830 g          499 t
ORIGIN      
        1 cttgcctccg aacatgtagc gaaacttcgc gatctggttg tacacctggt ccagacaaac
       61 cccgatgaaa tcggcgaaca tgatctcggc cacgggccgc aagcccgcga gcgccgcacc
      121 ggcggccgca ccggcgatcg cggattccga gatgggcgta tcgagcaccc gcccggcccc
      181 gaagcgcggg aggagtccct tggtgacccc gaagatccct ccagaagcct cgcgttgtcc
      241 ggaggtgccg ccggcgccgc cggacacatc ctcgcccaga acgatcacgc gctcatcgcg
      301 cgccatctcc tggtgcagag cctggttcag agcatcgcgc atggtgagct gaggcatggt
      361 ccccccggcg gcagatcaat actggatgta gacgtcggaa tacagatcgg cctcgccggg
      421 cggcgccgcg agcttggcct cggcgaccgc acgatccacg agctccagaa cctccgcgtc
      481 gatggcgtcc agcaccgcgc tcgacaccgt tgcgtccgcc cgggtgcgct cacgaaaccg
      541 cttcaggcaa tccatcgtgg cgcgatgccg ggacacctcg tccttggctc gatagttttg
      601 cggatcgcct tcgaaatgcc cgtagaagcg cgtggtcatc gcctcgatgg tgctcgggcc
      661 cccgccgctc tcggcgtgct ccacggcacg acccatggct tcgtagaccg cgtgaaaatc
      721 ggatccgtcg actcgctccg caggcattcc gaacgcgtgc gagcggctcg cgaggtcgcc
      781 cgatccgacg gcgtatcccg cgcccgtatg ctcgctgtag ccgttgttct ccatgacgaa
      841 aatcgcgggc accttgagca ccacggccat gttcatcgct tcgaacaccg tcccctggtt
      901 ggcgctgccg tcgccgccga acgcgacgct cacggagcgg ttgccgcgca gcttgcaagc
      961 gatggccgcg cccaccgcga tcggcggacc cccgcccacg atggcgttcg cgcccagcat
     1021 gccctttgca aaatccgcga tgtgcatcga gccgcccttg ccgccgcaga gcccctcctt
     1081 gcgcccgtag atctccttca tcatcccgac cacgtcgcaa cccttggcga tgcaatgccc
     1141 gtgcccccga tgagtgctga cgatgtagtc ctcatcgccg aggcggctgc acacgcccac
     1201 tgccaccgcc tcctggcccg catacaaatg cgtgaagccg gggatctcgc cggtcgcaat
     1261 ctcgacgtgc aaccgctcct cgaactcacg gatcgtcttc atttggcgat aggcggccgg
     1321 gagcagcagt tcggacggat tcctgttcat ctgtctgatc ctctggtcca ggactcgcgg
     1381 tcggcgcccg ccgggaacaa gtattcgtcg atcacctggt gaaagtgccg gatgcgcagc
     1441 tcctgccggc cgagccacag acccttgaag ccgcgcgaat gcattccggc ctgcacattc
     1501 ggcagattgg ccgcgtcctg gtccagcacc aggccgatcg acttgtcgcc gtgcctgaag
     1561 ctctgatgct tggcgcgctc cggccaggtc ttgccttccg gtacgagctc gtaggtccag
     1621 atgtcgtaat acatcttgtc gggatcgctc gcgtgcgggc gttgacgaaa tatccagaag
     1681 tcgtccgcat gcagattcag cgacagattc ggaaagatca gatagtgata gtcgtccgtg
     1741 agctgatcgt cgttgagctc ggaataatcc cgcccctgct gcgcacccac ggcacgcttt
     1801 tgcctctgca cgtcgcgccg gatgttcgcc accggctcgt cgtaatccgc cggatccatc
     1861 cccgcggact tcatgatcgt cttgatcgat tccggtatgc cggggggctt gcgcacccgc
     1921 ggagagacgg ttccgaaggg gatgagataa cggctgtggc gctcatagca gtcgatctgg
     1981 atgtccaggt cgtgcagatg ccagagcagc tgagggtgga tgccctgaac gtgatagctc
     2041 tcgttgaagg cgtcgacgga tgtcttccag ttgcaatccc attcgacggt gatgtcgcgg
     2101 gtctgcacca tccgctcgaa atgataaggg ttcaggtgca gcggaatcgg gctcagaaaa
     2161 tccgcgagcg gcgccgcctg cggatcgagg gagaaccaga cgaagctgcc ccactcctca
     2221 caacgcactt cgctcaggcc ataaggcggc gttccctgcg ggaacgtatc gacatccgga
     2281 atccgccggt agcttccgtc cagattgtat tcccagtgat ggtagaggca cttcaaggcg
     2341 agcgtcgggc cggtcgaccc gtggccgcag gacctcaatc ggttgccgcg gtgctgacag
     2401 acgttgtaga aggcgcgcac gttcccgtcg gcctgacgta tcacgatgat cgactcaggt
     2461 ccgatctccg tgaccagata gtcgcccggc ttcgcaatgt cgagcgaccg cccggccacg
     2521 agccatacgc gggtccacag gcgctcccac tcgagccgca tgaacgcccg ggaggtgtag
     2581 cgcagtggat cgatgctcgc ggtcccgaga tcgggatcgt ccgctttctc gtgcggagtt
     2641 ccaggcgtgt cggccgtgcg cccgtccgct ccgcgcttca cccgggacac cggcgcgtag
     2701 cgcggtgcag tcgatctcaa cag
//
LOCUS       DASZMC010000601         4048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3798857,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000601 DASZMC010000000
VERSION     DASZMC010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3798857"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VG274_12215"
     CDS             complement(<1..1331)
                     /locus_tag="VG274_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HWE07469.1"
                     /translation="MTDPIVIPAQQRRAVLSLGPVENCEEGWKFGLLDQHGQSLAILK
                     FRHEEGANQARAAVLEAVADAIPHQVAGHGPEGERNPEIAVLPGSAQADGGGARQWTH
                     TLLEEGQLDSFAGRVIEGLLITLIVANVAAVALETIPFVYSRFAPLFTAFEEFSLAAY
                     TIEYVARIWSAVEDPRIAVQGAWRGRLYFALRPLMVVDFLAFAPSFLGLFFGIDLRVL
                     RIFRLFRLLKLARYSQALQALLGVLLAERSALFASAILLLATTCLYGELMHLAEGAVQ
                     PNTLGTMPGAMYWAITTLTTVGYGDIAPMTPLGKFIAGIAMVTGLALFALPIGIIANG
                     FVTGLSRRRFAITWSMLRRQPLFHGFDVDSLNIILEAPTAMIVREHSQIIVEGRHADA
                     FYLVVSGVARVEQAGEEFDLGPGRMFGEQALYHSSTYDRTVTAETDMRVIT"
     gene            complement(1353..3086)
                     /locus_tag="VG274_12220"
     CDS             complement(1353..3086)
                     /locus_tag="VG274_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWE07470.1"
                     /translation="MRGKQSPTTPSTLLGLALRPHPRELAILVVTLLCVWVCELSIPF
                     LLGTTVDAAVSRAGGIGRIVRFGAATLAIAAVLYGVHVLYLRAEARLVARGTFRLRQL
                     AYTRLLDQPLFLPNGAGRGEIGHRLMSDADVIDAHAIYLLADLPFSALTVLGVLIVML
                     WMQPALALIVVTVLAAAAVLAEHVGRPLGAMEQSIRQHRASLGGRLQEALEGFRTIKL
                     FGRERYETGRLDADGHSLAGAETAAGLVVARLEPLLQLVETFGFLAVVWIGALFVFKS
                     ELSPGGLVAFIAYMELMRGPICSTGEYLAHYKQARSTLARMADLLSRLAPPLPGGTIE
                     IEGPLSVVLRDIHVAFSSAGRAILDGVSLAAVPGEIVAIVGDNGAGKSTLLDILLGLR
                     IPDSGTVLIGGKPLAEWNSEAWRSATAAVPQEVFLFRASLEENIRYGADADAREIATV
                     AEQAGLGAAIARMPLGIRTELSDNGANLSGGERQLTALARALVRRPRILVLDEPCSAL
                     DNAAVKNFNRVLGEEREGRITFIVAHDWDTIAHADRVVVLEGGRIAFEGSACEAASRV
                     DLFRRPARSAA"
     gene            complement(3088..>4048)
                     /locus_tag="VG274_12225"
     CDS             complement(3088..>4048)
                     /locus_tag="VG274_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWE07471.1"
                     /translation="SLTRGDTAVEATELPRFAPCDALPDRTAGASNDDLYIDHESASG
                     GHWFRCDGTVATVRTPFQLAEIVEFAHFGRALVLDGALQSVERDEYIYHEALVQPALC
                     LHAKPRRVLIVGGGEGATAREVLKHATVEEVVMVDIDGELIALAREHLQTWHRGSFDD
                     PRLRLRIGDGYGFITATADRFDVIIIDLVDFFEGGPAESLYTTEFYRVLKRRLAPDGI
                     LVVQAMECDASELADHLRVRRNLAGLFSHMRSYLAFVPSFWSTWGFVIASDAIDPHAA
                     SPGLIDHVITERALTQRLRFYDGKTHQAFFALPKDLRTLLGEA"
BASE COUNT          812 a         1282 c         1264 g          690 t
ORIGIN      
        1 aacgtgatca cgcgcatgtc cgtttcagcc gtcaccgtcc ggtcgtaggt gctcgaatgg
       61 taaagcgcct gctcgccgaa cattctgccc ggaccgagat cgaactcctc gccggcctgt
      121 tcgactcgcg cgacgcccga cacgacaaga tagaaagcgt ctgcgtgtct gccctccacg
      181 ataatctgcg aatgttcgcg cacgatcatg gccgtcgggg cttcgaggat tatgttcaat
      241 gaatctacat cgaaaccgtg gaacagcggc tggcgcctca gcatgctcca ggtgatggcg
      301 aagcggcgcc ggctgagacc ggtgacgaag ccgttggcaa tgatgccgat gggcagcgcg
      361 aagagggcaa ggcctgtcac catcgcgatg ccggcgatga attttcccag tggtgtcatg
      421 ggcgcgatgt cgccgtaacc gaccgtagtg agcgtcgtga ttgcccagta catcgcaccg
      481 ggcatggttc cgagcgtgtt gggttgaacc gctccttccg cgagatgcat cagttcgcca
      541 tacaggcagg tcgttgcgag caggaggatt gcggatgcga acagtgccga tcgttccgca
      601 agcagcacgc cgagaagtgc ctgaagcgcc tgggaatagc gcgccagctt caacaggcgg
      661 aacagccgga agattcgcaa cacgcgcagg tcgatgccga aaaacagtcc gagaaagctc
      721 ggtgcgaagg ccaggaagtc gacgaccatc agcggccgca atgcgaagta gagacgtccg
      781 cgccacgcgc cctgcaccgc gatgcgcggg tcttccaccg cggaccagat tcgcgcgacg
      841 tattcgatcg tgtacgcggc cagcgaaaat tcctcgaatg cggtgaacaa cggagcaaac
      901 cgcgagtaga cgaaagggat cgtctcgagg gcgacggccg ccacgttcgc gacaatgagc
      961 gtgatcagca atccttcgat gacgcggccg gcgaagctgt ccaactgtcc ctcttccagc
     1021 agcgtgtgcg tccattgcct cgcgccgccg ccatccgctt gtgcagagcc gggcaggacc
     1081 gcaatttcgg ggttgcgctc accttcgggg ccgtgcccgg ccacctggtg gggaattgcg
     1141 tcggcaaccg cctcgagcac ggccgctctt gcctggttgg ccccctcctc gtggcggaat
     1201 ttcagtattg cgagcgactg accgtgctgg tcgagaagtc cgaatttcca accctcctcg
     1261 caattctcga ctggccccag cgaaagcacg gcgcgccgct gctgtgcggg aattacgatt
     1321 ggatcggtca aattgattcc cccatcgctc ctctaggctg cgcttcgcgc cgggcgacga
     1381 aagagatcca cacgactcgc agcttcgcag gcgctgcctt cgaaggcgat ccttccccct
     1441 tcgagaacga cgacacgatc ggcgtgcgcg atcgtatccc agtcatgagc cacaatgaat
     1501 gtgatgcgcc cctcccgctc ttcgccaagc acgcgattga agttcttgac ggcggcgttg
     1561 tcgagggccg aacacggctc gtcgagaaca agaattcgcg gcctgcgcac caatgcccgg
     1621 gcaagggcgg tcagctggcg ttcgccgccg gacaggttcg caccattatc gctcagctcg
     1681 gtgcggatgc cgaggggcat ccgggctatc gcggcgccaa gccccgcctg ctcggccacc
     1741 gtggcgatct cccgcgcgtc cgcatcggcg ccgtagcgga tgttctcttc gagcgaggca
     1801 cggaagagaa acacctcctg cgggaccgcg gctgtcgcgg aacgccaggc ttccgaattc
     1861 cattcggcga gcggcttgcc tccgatcagg acggtcccgc tgtccggaat ccgcaggccc
     1921 agcagaatgt ccagaagcgt cgattttccc gcgccgttgt cgccaacgat cgcgacgatc
     1981 tcgccgggca ctgcggcgag cgatacgcca tccagaatcg cgcgacctgc tgaggaaaaa
     2041 gcaacatgga tatcgcgcag cacaacgctg agcggacctt cgatctcgat cgtgccgccg
     2101 ggaagcggcg gcgcgaggcg tgaaagcaga tcggccatgc gcgccagtgt ggaacgcgcc
     2161 tgtttgtaat gcgcgagata ttcgcccgtg ctgcatatgg gccctctcat cagctccatg
     2221 tacgcgatga acgccacgag cccgcccggc gacagctcgg atttgaaaac gaagagcgcg
     2281 ccgatccaca ccaccgcaag aaatccaaac gtttccacca gttgcagcag cggctcgagc
     2341 cgggcaacta ccagccccgc cgccgtctcc gcgcctgcca ggctatgccc gtcggcgtcg
     2401 agcctcccgg tttcatatcg ctcgcgcccg aaaagcttga tggtgcgaaa tccttccaac
     2461 gcttcctgca atcgcccccc gaggctggca cggtgttggc gaatcgattg ctccattgcg
     2521 cccaggggac gtccaacatg ctccgccagc accgcggcag cggcgagaac cgtcacgaca
     2581 atcagtgcaa gcgcgggttg catccagagc atgacgatca ggacgcccag cacggtcagt
     2641 gcagagaacg ggaggtccgc gagcaaatag atcgcgtgcg cgtcgatcac gtctgcatcg
     2701 ctcatgaggc ggtgaccgat ttcgcccctt ccggcgccgt tcggcaggaa caggggttgg
     2761 tcgagcaggc gcgtgtaggc gagttggcgc agcctgaacg tgccacgcgc cacaagcctt
     2821 gcctcggcac gcagataaag cacgtgaact ccgtaaagca ctgcggcgat ggccagcgtc
     2881 gccgctccga agcggacaat gcggccaatg cctccggccc tggagaccgc ggcatcgacg
     2941 gtcgtgccca gcaggaacgg aatcgagagt tcgcaaaccc agacgcaaag caatgtcacg
     3001 acaagaattg cgagttcgcg cggatgcggt ctcagtgcga ggccgagcag cgtggacggc
     3061 gtcgtcggac tctgcttgcc gcgcatgtca cgcctcgccc agaagtgtgc gcagatcctt
     3121 gggcaaggcg aagaacgcct gatgcgtttt gccgtcgtag aacctgagcc gttgcgtcaa
     3181 agcacgctcg gtgatgacgt gatcgatcaa accgggcgaa gcggcatgcg gatcgattgc
     3241 gtcggaagcg atgacaaagc cccacgtcga ccagaaagac ggcacgaacg ccagatagct
     3301 gcgcatatgc gagaacagac cggcaagatt gcggcgcacg cgcagatgat cggctaattc
     3361 gctcgcgtcg cactccattg cctggacgac gaggattccg tccggagcaa gccgccgctt
     3421 caggacccga tagaactccg tcgtgtagag cgattccgcg ggacctcctt cgaagaaatc
     3481 gacgagatcg atgatgatca cgtcgaagcg gtccgccgtt gccgtaatga agccgtaacc
     3541 gtcgccgatg cgaaggcgca gccggggatc gtcgaaactg ccccggtgcc aggtctgcag
     3601 atgctcgcgc gcaagtgcga tgagctcgcc gtcgatatcg accatcacca cttcctctac
     3661 ggtggcgtgt ttaagcacct cgcgggcggt tgcaccttcg cctccgccga cgatcagcac
     3721 acgccggggt ttggcgtgaa ggcagagcgc cggttgcacc agcgcctcgt ggtagatgta
     3781 ctcgtcgcgt tcgacggact gcagggctcc gtcgagaacg agcgctcgcc cgaaatgcgc
     3841 gaattcgaca atctcagcca gttggaatgg cgtacggacc gttgcaaccg ttccatcgca
     3901 tctgaaccaa tgaccaccac tcgcgctttc atggtcgatg taaagatcgt cgttcgaagc
     3961 gccggccgtg cgatcgggca gggcatcgca gggcgcgaag cgcggcaatt cggtggcttc
     4021 gacggcggtg tcacctcggg tgaggctg
//
LOCUS       DASZMC010000602         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1644065,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000602 DASZMC010000000
VERSION     DASZMC010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644065"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VG274_12230"
     CDS             <1..201
                     /locus_tag="VG274_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07472.1"
                     /translation="LVGDEAYYQRVGFVRLAPGRVRFPGPVDKTRVLGLALEEDALQI
                     LEGDITRARIDAPVCADSARLG"
     gene            complement(198..617)
                     /locus_tag="VG274_12235"
     CDS             complement(198..617)
                     /locus_tag="VG274_12235"
                     /inference="COORDINATES: protein motif:HMM:NF017338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWE07473.1"
                     /translation="MSFSELISPLLGRLALAWFFLSEVYTRAGAWDANVSLMAMLNIP
                     APPLLLAVALIVMLLGGLSLLFGFHARHGAMLLFGFVAVATFLMHDYWQIKNVTDRAA
                     DYELFARNVAIAGGLLMVIGLGAGPFAIDNTNQKKRR"
     gene            complement(687..1115)
                     /locus_tag="VG274_12240"
     CDS             complement(687..1115)
                     /locus_tag="VG274_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HWE07474.1"
                     /translation="MKRTNTLIRLRRFRVDDLKRRLATLDGMKADAERKLADLEDSVT
                     REKQRAGDSDIGRLAFPSFLRSIESRRENLRETLKDLERERVQAQDELTSAFQDLKAL
                     EFATEQQAKRANELEARRAQSRIDEMALVRHLRKHALRGI"
     gene            complement(1112..>1621)
                     /locus_tag="VG274_12245"
     CDS             complement(1112..>1621)
                     /locus_tag="VG274_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07475.1"
                     /translation="TKGYTPTVFAELPRLLERAGPGPQDCHGSITGLFTVLVEGDDHN
                     EPIADAVRGILDGHIVLERNIAERGRFPAINILKSVSRAMPGCNNAREQSVVADARAP
                     MALYEDMAELIRLGAYKMGSNPDVDTAIALNPRLEAFLIQKKDERSTLADGYNALEKI
                     MGDGKGRVT"
BASE COUNT          312 a          506 c          493 g          310 t
ORIGIN      
        1 ctcgtcggcg atgaagccta ttatcagcgt gtgggatttg tgcgcctcgc gccggggcgc
       61 gtacgctttc ccggacccgt cgataagaca cgtgtgctgg gactggcgct tgaggaagac
      121 gcgctgcaaa tccttgaagg cgacatcacc cgcgcgcgca tcgacgcccc ggtgtgtgcc
      181 gacagcgcgc ggctcggcta acgacgcttt ttctggttgg tgttgtcgat cgcaaacggt
      241 ccggcgccca acccaatcac catcagaagc cctccggcaa tcgcgacgtt gcgcgcaaag
      301 agttcatagt cggcggcgcg atcggtcacg ttcttgatct gccaataatc gtgcatcagg
      361 aatgtcgcca cggcgacgaa gccaaataga agcatcgcgc catgccgggc atgaaagccg
      421 aacagaagcg acagcccgcc gagaagcatc acgatcaacg ccaccgccag cagcagaggc
      481 ggcgcgggga tgttcagcat cgccatcaac gaaacgttcg cgtcccacgc gcccgcgcgc
      541 gtatagacct ccgacagaaa aaaccacgcc agcgccaacc gccccagcaa tggcgaaatc
      601 agttccgaga aagacatgtg cgcccccgcg caaatcattt cgcggggagt ctaaggccaa
      661 ccggcgcgcg ctggaaatgg ccgcgcttaa atgcccctga gcgcgtgttt tctgagatgc
      721 cgcaccagcg ccatttcgtc gatgcgggac tgggcgcggc gggcttcgag ttcgttggcg
      781 cgcttggcct gctgctcggt cgcgaattcc agggctttga gatcctgaaa cgcactggtg
      841 agctcgtcct gcgcctgcac gcgctcgcgc tcgaggtcct tgagcgtttc gcgcagattc
      901 tcgcggcggg attcgatcga ccgcaggaac gacggaaacg cgaggcgtcc gatatctgag
      961 tcgccggcac gctgcttctc gcgcgtgacg ctgtcctcga gatcggcaag cttgcgctcg
     1021 gcgtcggctt tcatgccgtc gagcgtggcc aggcggcgct tcagatcgtc gacgcgaaag
     1081 cggcgcaaac ggatcagcgt gttggtgcgt ttcatgtgac cctacccttc ccgtcgccca
     1141 tgattttttc gagggcgtta tatccatccg ccaatgtcga acgctcgtcc ttcttctgta
     1201 tgagaaaggc ttcgagccgc ggattgagtg caatagcggt atcgacgtcg ggattgctgc
     1261 ccatcttata ggcaccaagc cggatcaatt ccgccatgtc ttcgtaaagc gccatcggtg
     1321 cgcgcgcatc ggccacgacg ctctgctcgc gggcattgtt gcagcccggc atggcgcgcg
     1381 aaaccgattt gagaatgttg atcgcgggaa aacgtccgcg ctccgcgatg tttcgttcca
     1441 gcacaatgtg accgtcgaga atgccgcgca cggcgtccgc gatcggttcg ttgtgatcgt
     1501 cgccttcgac gagaaccgtg aacaatccag tgatggaacc atggcaatcc tgcggtccgg
     1561 gaccggcgcg ctccagcaag cgcggcaatt ccgcgaacac agtcggcgtg taacctttcg
     1621 t
//
LOCUS       DASZMC010000603         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2999391,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000603 DASZMC010000000
VERSION     DASZMC010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2999391"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1258)
                     /locus_tag="VG274_12250"
     CDS             complement(65..1258)
                     /locus_tag="VG274_12250"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWE07476.1"
                     /translation="MPFPRASQALSRFTVLDLTRVRAGPTCVRQLADWGANVIKIDAI
                     LEDAGSEQLGGPRHGADFQNLHRNKRAISLNLKDPKGLEVFNRLVAKADVVVENFRPD
                     VKAKLGIDYESVRNVNPRIVYGSISGFGQDGPYHKRPGFDQIAQGMGGLMSITGAPGE
                     GPMRVGIPVADLTAGLFCAMGILTALLERDISGEGQWVRTSLLQAQIFMLDFQAARWL
                     MEKDVAQQAGNDHPTSIPTGVFRTSDGYINIATTGGRIWTRFCEALGAPDLPNRPEYA
                     TAPERSKNRKALNAEINKHTEKKSSEAWLKELNAAGVPCGPIYSIDQVFADAQVEHLG
                     IAQHVPNDENRHIQLVGQPFTLSRTPSKMAARPPEFGEQTEEVLAEFGFGQEEIADLR
                     QHKVV"
BASE COUNT          262 a          467 c          505 g          298 t
ORIGIN      
        1 atacaaagag ccccggattc ccggggcttt ttcatcacgt tgagggcagg ccgggcgccg
       61 cacgtcacac caccttgtgc tgcctcaaat ccgcgatctc ttcctggccg aagccgaact
      121 cggccagcac ttcctcggtc tgctcgccga actccggcgg ccgcgccgcc atcttgctcg
      181 gcgtgcgcga cagcgtgaag ggctggccga ccagctgaat atgccggttc tcgtcgttcg
      241 gcacgtgctg ggcgatgccg agatgctcga cttgcgcatc ggcgaatacc tggtcgatgg
      301 aatagatcgg gccgcagggc acgccggcgg cgttcaactc cttgagccag gcctcgctcg
      361 atttcttctc ggtgtgtttg ttgatctcgg cgttgagtgc ttttcggttc ttcgagcgct
      421 ccggcgcagt ggcgtattcg ggtcggttcg gcaggtcggg cgcgccgagc gcctcgcaga
      481 agcgagtcca gatccggccg cccgtggtcg cgatattgat atagccgtcg gaagtcctga
      541 acacgccggt cgggatcgag gtcggatggt cgttgccggc ctgttgggct acgtcttttt
      601 ccatcagcca gcgcgcggcc tggaagtcca gcatgaagat ctgcgcctgc agcagcgagg
      661 tcctaaccca ctggccttcg cccgaaatgt cgcgctcgag cagcgcggtg aggatgccca
      721 tcgcgcagaa cagccccgcg gtgaggtcgg ccacgggaat gccgacccgc atcggtccct
      781 cgccgggcgc gccggtgatc gacatcagcc cgcccatgcc ctgcgcgatc tgatcgaagc
      841 cggggcgctt gtggtagggg ccgtcctggc cgaagcctga gatgctgcca tagacgatgc
      901 gcgggttgac gttgcgcaca ctctcatagt cgatgccgag cttggccttg acgtcgggac
      961 ggaaattctc gaccaccacg tcggccttgg cgaccagccg gttgaatact tcgagcccct
     1021 tcgggtcctt caaattcagc gagatcgccc gtttgttgcg atgcaaattc tggaagtcgg
     1081 cgccgtggcg cggtccgccc aattgctcgc tgccggcgtc ttccaaaatc gcgtcgatct
     1141 tgatgacgtt ggcgccccaa tccgccagct gccgcacgca ggtggggccg gcacggaccc
     1201 gcgtcaggtc gagcacggtg aagcgggaca aagcttgcga ggcacgcggg aagggcattc
     1261 aggaactccg ggaccaaagg cgggaacggc gcgctttttg atcggtcaga ttgaggtttt
     1321 attcaatagc caatctcggg gagccaggcc gcatcgcggc tgcgtccgaa gtctccgagg
     1381 acatcagcgc gaatcgatat gcaagagcgt aaggtgcggt tgacttgggg cgcgggggca
     1441 tgcggcggtc gagggcggtc tggggcagca actgcagagc tagtgctttt cccgaccgga
     1501 tgtttcgaat gaaatagcca ataaacggca aa
//
LOCUS       DASZMC010000604         9830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2449532,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000604 DASZMC010000000
VERSION     DASZMC010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449532"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VG274_12255"
     CDS             <1..1995
                     /locus_tag="VG274_12255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWE07477.1"
                     /translation="PYVIRQVGASRNKLALTFDDGPDPQWTPQILDILKAKNVKATFF
                     VIGENAEAYPEIVQRMVAEGHDVGNHTFTHPNLADTPGPAVVLELNATQRLFQALTGR
                     SLRLFRPPYLGDAEPSDEPELRPVELAQSLGYITVGMHADPVDWALPGTDAILQRTLA
                     AVHAKNPDQRANIILFHDAGGDRSQTVAALPNVIDTLRAQGYQFVPVAQLAGLTRAQA
                     MPRLPVTATLIMDRLVFLALSWTGVILYYCFLTAIFLGIGRLLVWCVLALFNRPREAE
                     QAAPPASANPRRVSVIIPAFNEEKVIATTIEQILASGHRNIEVLVVDDGSRDATAAIV
                     EQQFGADPRVELLRIANGGKANALNTGLARAQGEVVVALDADTQFCGDTISRLVRWFA
                     DPHVGAVAGNAKVGNRINTITRWQALEYIVSQNLERRALAALDTLTVVPGAVGAWRRS
                     VLEELGGFTASTLAEDQDLTIAVQRAGYRVLFDASAIAWTEAPATVRGFAKQRFRWAF
                     GTLQCLWKYRGMTFNPRCGALGLVALPQAWLFQIFLTALAPVADLMLIWQLLFQSIAY
                     LEHGAEFSNTNLLTVGVYYAVFMVVDLLAALFGFCMERRENWNLTWWLMLQRFGYRQI
                     MYYVLVRSISTALRGRFVGWSKLERTGTVKVNYARDLPAH"
     gene            complement(2023..3480)
                     /locus_tag="VG274_12260"
     CDS             complement(2023..3480)
                     /locus_tag="VG274_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07478.1"
                     /translation="MSASRDEGDARANALDHALAPMLPVLLMAAALLCVAGVARAIAV
                     LPLHVSLDPNEGWNAYHAAAAIAGHGLYPSAAGLMANNYPPLSFYLVGALGRLLGDHI
                     IAGRVISLASFLCCSWFIAIAIGRMKGAWQASTFAPLLFAGILLVTSDYVGMDDPELF
                     GHALQLAALLFLLREPRGASAIFASACLFVAGGFVKHNLFALPLAAQIWLALYDRRNA
                     LRFAVWIAALSVAGLLAFRVTFGFDLLDRLNSARAYSVAQLRGNISSWLEVGLIPGCA
                     LLGLTIWRKRDRFVALLALYAVMSVAAGAWLLGGAGVDVNAMFDADIALALGAGLALE
                     MLTRRRDPLPHIASRALAFFCLLPLAMLAFTVSDWRDPAYWLHPMRDESALASDGIAF
                     LRNHRGPAMCENLTLCYWAGKPAEVDMFNLDQQFVTHARKPAPFISLLDARHFSAIEL
                     DETDPFPLPPGAQDAIRRNYRIDHVNDEGVFFLPK"
     gene            complement(3477..5066)
                     /locus_tag="VG274_12265"
     CDS             complement(3477..5066)
                     /locus_tag="VG274_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07479.1"
                     /translation="MGSKPDPARIRASIGGLGPPWHALALFAAALLLVLPIWLVRMPA
                     MPDYPAHLASFHLLAGGAKSYPLSHFYRVQWAFVPNLASEAIVPCLSHLVGFAQATAI
                     FLSLTVATWVLGAGAVHWALYRRIGAAPLFACFFAYNANFMWGFFNYAFGMGCGLFGF
                     AGWIADRNRGRPIHLAGFALATLAIYFCHVFAAATLLVLVACHEVSGCDRPISFGAAL
                     KRLANVAVLAAPSALAFVLLRPKGAGGAITFNLLDTIIDRLSAAVQFTFDQPAWLLLA
                     ALAALFAVGVWRGKIFVHPRMRFGLVVLAVACVFAPEWAMGGWGVDLRLPAVLGTIAF
                     ASADFRFQRRTEATILVAALVVAAWSAATLTGNWRYYDRRFAEFRAAASKIEPGAKLV
                     TVLDGDAMGLASDQPYWHMAEYAIIDRGAFTPLLFTTAGQHVIRLQPAVAGIAANSAQ
                     QGSPPDISELDDLAAGNVADDKDIREVFPYLIRFQCHYDVAVVVHLGGRRSRVPDMLE
                     LRHAGSFFSLYRIRREDCAKQ"
     gene            5294..6244
                     /gene="ispH"
                     /locus_tag="VG274_12270"
                     /gene_synonym="lytB"
     CDS             5294..6244
                     /gene="ispH"
                     /locus_tag="VG274_12270"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017387043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWE07480.1"
                     /translation="MRVILAQPRGFCAGVVRAIDIVEHALEKYGEPVYVRHEIVHNRH
                     VVETLKRKGACFVEELDEVPHGAVTVFSAHGVPQAIVREAARRELPVLDATCPLVSKV
                     HAQGKRYVSKGRTLVLIGHAGHPEVEGTMGQVGAPVHLVSSEADVQALDLPDDAPVAY
                     VTQTTLSIDDTRGVIAALKRRFSDIVGPDTSDICYATQNRQSAVRELCKIAEVILVVG
                     AKNSSNSNRLREIGLEEGVPSYLIADGSELDPAWVAGKLTIGLTAGASAPEELVRNVI
                     DALRLLGEVEVSEMDGVEEKIEFRLPAELRNRKRQSASVE"
     gene            6248..7396
                     /gene="hpnH"
                     /locus_tag="VG274_12275"
     CDS             6248..7396
                     /gene="hpnH"
                     /locus_tag="VG274_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HWE07481.1"
                     /translation="MGIPLRQAVRVGAYVMKQHLAGRRRYPLVLMLEPLFRCNLACAG
                     CGKIDYPDEILNQRLSYDECMQAIDECGAPVVSIAGGEPLLHRELPEIVRGFLERKKF
                     VILCTNALLLAKKIDQFRPHRDFTWSIHLDGDKAMHDKSVCLEGTYEKAVDAIRLAKS
                     KGFRVSINCTLFSDAQPERVAAFFDEMKAIGLDGMTISPGYAYERAPDQEHFLNRERT
                     KNLFRGIFSRGDGGRKWPISQSAMFLDFLAGNQTYRCTPWGNPTRTVFGWQRPCYLLG
                     EGYAPTFRELMEETDWDKYGVGNYEKCANCMVHCGFEATAVIDSVKRPLAALGVMLKG
                     VRTEGQMAPEIPLDRQRPAQFVFSSHVERKLAEIRNAKPANKQVAAAE"
     gene            complement(7319..8005)
                     /locus_tag="VG274_12280"
     CDS             complement(7319..8005)
                     /locus_tag="VG274_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07482.1"
                     /translation="MIVAVTGLAREARLIANPKIVSVVGGGDAVSLERRLADELGKRA
                     RRILSIGICGALSPELRVGDVIVASEVVADSESIPTHAPWTRELALRVPGAVVAPLAG
                     IDTIAGHRDAKAKLYAATRASVADMESHVAARAARSRGLPFAALRVVSDAANRTLPPA
                     ACVALHPSGGIDIVGVMRSLLRAPQQIPALLRTAWEAEIAFAALFRCRHLLVGGFGVA
                     NLGELALDVG"
     gene            complement(8005..>9830)
                     /gene="shc"
                     /locus_tag="VG274_12285"
     CDS             complement(8005..>9830)
                     /gene="shc"
                     /locus_tag="VG274_12285"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWE07483.1"
                     /translation="TIPAEYVLLAHYLGETPNPELELRVGKYLRRIQADHGGWPLYHG
                     GAFDISATVKAYFALKMIGDLPEAPHMSRAREELLARGGAARSNVFTRILLALFGESS
                     WRNVPTIPVEIILLPRWFPIHLSKMSYWARTVIVPLLVLATLRPLARNPRGVHIRELF
                     AGSGAPPPLRAPHQNPYWAVLFSVLDRILKLVEALWPARLRKRAIARCMEFVSERLNG
                     EDGLGAIYPAMANSVMMYDALGYPPDHAERALARKSVENLLVHRADETYCQPCVSPVW
                     DTALAAHALMESRCANADVRIGAMCEWLKARQVLHVKGDWAEERPDARPGGWAFQYNN
                     AHYPDLDDTAVVVMTLDRARGIGRAHSDYSEAIARGCEWVAGLQSHNGGWAAFDADNT
                     YYYLNNIPFADHGALLDPPTADVTARCIGMFAQLGEASDSPSMKAAVDFMARVQEKDG
                     SWFGRWGVNYIYGTWSALAGLNAAGFDPHSPTIRRAADWLIAIQNDDGGWGESCDSYR
                     LDYRGYEAAPSAPSQTAWALLGLMAAGRVDHPAVARGIGFLQKRQDDQGLWPEEGCTG
                     GGFPRVFYLRYHGYSKFFPLWAVARYRNLRAGNSNRVSYGI"
BASE COUNT         2012 a         3078 c         3030 g         1710 t
ORIGIN      
        1 ccgtacgtca tccgccaggt gggggccagc cgcaacaagc tcgcgctcac tttcgacgat
       61 ggtccggatc cgcaatggac tccgcagatc ctggacatcc tgaaagcgaa aaacgtcaaa
      121 gcgacatttt tcgtcatcgg agaaaatgcg gaagcctatc ccgaaatcgt gcagcgcatg
      181 gtcgcggagg gacacgacgt cggcaaccac actttcaccc atcccaacct cgccgacacg
      241 ccgggccccg cggttgtgct ggaactgaac gccacccaga ggctcttcca ggcgctgacc
      301 ggacgttcgc tgcggctgtt ccgcccgccc tatctgggcg acgcggagcc ctccgacgag
      361 cccgaacttc gtcccgtgga gctggctcag agcctcggtt acataaccgt cggaatgcac
      421 gccgatcccg ttgactgggc gctgccggga accgacgcaa tcctgcagcg cacgctcgcc
      481 gcggttcatg cgaagaaccc tgatcagcgc gccaacatca tcctgtttca cgacgccgga
      541 ggcgatcgct cgcagaccgt ggcggcgcta cccaacgtga tcgacacgtt gcgggctcag
      601 ggctatcagt tcgtgccggt cgcccaactt gccggactga cacgtgctca ggccatgccg
      661 cgcctgccgg tgacagcgac gctcatcatg gaccggcttg tcttccttgc actcagctgg
      721 accggcgtca ttctttacta ttgctttctg acggcgatct tcctgggaat aggcaggctg
      781 ctcgtctggt gcgtactcgc actcttcaat cggccgcgtg aagccgaaca ggcagcgcca
      841 cctgcatccg ccaatccccg ccgcgtatcg gtgatcattc ccgccttcaa cgaagagaag
      901 gtgatcgcga cgacgatcga gcaaatactc gccagcggtc atcgcaacat cgaagtgctc
      961 gtagtggatg acggctcgcg cgacgcaacc gccgcaatcg tcgagcagca attcggcgcc
     1021 gacccgcgcg tcgagcttct gcgcatcgcc aatggcggca aggccaacgc gctcaacaca
     1081 gggcttgcgc gcgcgcaagg cgaagtggtc gtggcgctcg atgccgacac gcagttttgc
     1141 ggcgacacga tctcgcggct ggtgcgctgg ttcgccgatc cgcatgttgg cgccgtggcc
     1201 ggcaacgcca aagtcggcaa ccgcatcaac acaatcacgc gctggcaggc gctcgaatat
     1261 atcgtgtccc agaatctcga acgtcgcgcg ctggccgctc tcgacacgct gaccgtggtt
     1321 cccggcgcag tcggcgcgtg gcgccgcagc gtgctcgagg agctcggtgg ctttaccgcg
     1381 agcacgctcg ccgaggacca ggacctgacg attgcggtgc agcgtgcggg atatcgcgtg
     1441 ctgttcgacg cgagcgccat cgcctggacc gaggcgcccg ccacggtccg cggtttcgcc
     1501 aagcagcgtt tccgctgggc tttcgggaca ctgcaatgtc tgtggaaata tcgcggcatg
     1561 acgttcaatc cgcgctgcgg tgcacttgga ctcgtcgcct tgccccaggc gtggctgttc
     1621 cagatttttc tgactgctct cgcgccggtc gcggacctca tgctgatctg gcaattgctg
     1681 ttccaatcaa tcgcctatct cgagcacggc gcggagttca gcaacaccaa tctgctcacc
     1741 gtcggtgtct actatgcggt gttcatggtc gtcgatctcc tcgccgcgct cttcggcttc
     1801 tgcatggagc ggcgcgagaa ctggaacctg acctggtggc tgatgctgca gcgcttcggg
     1861 tatcgccaga tcatgtatta cgtgctcgtg cgctccatct ccacggcgtt gcgcggccgg
     1921 ttcgtcggct ggtccaagct cgagcgcacc ggaaccgtaa aggtgaatta cgcgcgcgat
     1981 ttgccggcgc attgaaggcc tcgggccggc gcgctcattc aattacttcg gaaggaagaa
     2041 taccccctcg tcgttgacgt gatcgatgcg atagttgcgg cggatcgcgt cctgcgcacc
     2101 ggggggcagc ggaaacgggt ccgtttcgtc aagctcgata gccgagaaat ggcgcgcgtc
     2161 gagcaggctg ataaacgggg ccggtttgcg cgcatgggtg acgaattgct ggtcgagatt
     2221 gaacatgtcg acctccgctg gttttccggc ccagtagcac aatgtcagat tctcgcacat
     2281 cgccggtccc cgatggttgc gcaggaatgc gatgccgtca ctcgccagcg ccgattcatc
     2341 tcgcatcgga tgcagccagt atgccggatc gcgccaatct gaaactgtga acgcaagcat
     2401 cgctaacggc aacaggcaga aaaatgcgag cgcgcggctc gcgatgtgcg gcagggggtc
     2461 tctccggcgc gtgagcatct ccagcgcgag tcccgcaccg agcgcgagcg caatatcggc
     2521 gtcgaacatc gcgttgacat cgacgcccgc gccgccgagc aaccaggcgc ccgcagcaac
     2581 cgacatcacc gcgtaaaggg cgagcagcgc gacaaaccgg tcgcgcttgc gccagatcgt
     2641 caaacccagg agcgcgcagc ccgggatcaa tccgacctcc agccagctcg atatgtttcc
     2701 gcgcaactgg gcaaccgaat aggcgcgtgc cgaattgagg cgatcaagca ggtcgaaccc
     2761 gaaagtgacg cggaatgcca ataggcccgc aaccgacagc gccgcgatcc agaccgcgaa
     2821 tcgcaatgca ttgcggcggt cgtagagcgc caaccagatc tgcgccgcga gcggcagcgc
     2881 aaagagattg tgcttcacga aacccccggc cacaaagagg caagcacttg cgaatatcgc
     2941 cgatgctccg cgcggttcgc gcaacaaaaa gagaagcgcc gcaagctgga gtgcgtgtcc
     3001 gaaaagctca ggatcatcca ttcccacata gtcggacgta accagcagaa tcccggcaaa
     3061 gagcagcggc gcaaaggtgc tcgcctgcca cgcgcccttc atgcggccaa ttgcaatcgc
     3121 gatgaaccag gaacagcaaa ggaacgacgc aagagaaatg acgcgtcccg caatgatgtg
     3181 atcgcccagc aacctcccca gagcgccgac caaatagaag gaaagcggcg gatagttgtt
     3241 tgccatcaag ccggcggcag atggatagag gccgtgtccg gcaatggctg ccgctgcgtg
     3301 gtaggcgttc cagccctcat tcggatcaag cgagacgtgg aggggtaaga ccgcaatcgc
     3361 ccgcgcaacg cctgcaacgc acaacaacgc ggcagccatg aggagaacgg gcagcattgg
     3421 cgcaagcgcg tggtccagcg cgttcgcgcg cgcgtctcct tcgtcgcgag aagcgctcat
     3481 tgcttggcgc aatcttcacg gcgaatgcgg tatagagaga agaagctgcc ggcatgtcga
     3541 agctcgagca tgtcaggcac gcgactcctg cgtccgccga gatgcaccac gacggcgacg
     3601 tcgtagtggc actggaaccg gatcaggtat gggaagacct cccggatatc cttgtcgtcg
     3661 gcgacgtttc cggcggcgag atcgtcaagt tccgaaatgt cggggggcga gccttgctgc
     3721 gcggagttcg cggcaatgcc tgcaacggcc ggctgcagcc ggatcacatg ctggcccgca
     3781 gtcgtgaaga gcagaggcgt gaaggctcca cggtcgatga tcgcatattc cgccatgtgc
     3841 cagtagggct ggtcggaggc gagccccatc gcatcgccgt cgagcaccgt cacaagcttt
     3901 gcaccgggct cgattttgct ggctgcggcg cgaaattccg cgaatcgccg gtcgtagtag
     3961 cgccaattgc cggtcagcgt tgcggcactc cacgccgcaa cgacaagcgc cgccacgaga
     4021 atggtcgcct ccgttcggcg ctggaagcga aaatcggcgg acgcgaacgc gatggttcca
     4081 agtacggcag gcagacgcag atcgacgccc cagccgccca tcgcccactc gggcgcgaag
     4141 acacaggcga cggcgagtac aacaagtccg aacctcatgc gcggatgcac gaaaattttt
     4201 ccgcgccaca cgcccactgc aaaaagcgcg gccagcgccg caagcaaaag ccaggctggc
     4261 tggtcaaagg tgaattgcac cgccgcactc agtcgatcga tgatcgtatc gagcaggttg
     4321 aatgtgatcg ccccgcccgc gccttttggc ctgaggagaa caaaggcgag agccgacgga
     4381 gctgcgagta ccgcgacatt tgccaagcgc tttagtgccg ctccaaatga aatcgggcgg
     4441 tcacagcctg atacttcgtg gcacgcgaca agaacgagaa gcgttgccgc ggcgaaaacg
     4501 tgacagaaat agattgccaa cgtagcgagg gcgaagccgg caaggtggat cggcctgcca
     4561 cgatttctat ccgcaatcca gccagcgaaa ccgaaaaggc cgcaccccat gccaaaggcg
     4621 tagttgaaga agccccacat gaaattcgcg ttgtaggcga aaaagcaggc gaagagcgga
     4681 gcggcgccga tgcgacggta gagtgcccaa tgcacggctc cggcacccaa aacccatgtg
     4741 gcgacagtca ggctgaggaa aattgcggtt gcctgcgcga agccgaccag gtgcgaaagg
     4801 cagggcacaa tcgcttccga cgcaagattc ggcacgaacg cccattgcac gcggtaaaag
     4861 tgtgagagcg gataggattt cgcgccgccc gccaggagat ggaaactcgc cagatgcgcc
     4921 ggataatccg gcatggcggg catgcgcacc agccagatgg gcagcacgag caggagtgcg
     4981 gcggcgaaaa gggccagtgc atgccatggc ggcccaaggc caccaatact ggccctgatg
     5041 cgcgccggat caggttttga ccccattgtg atcgtttttc tgtccccttt tgttccggtc
     5101 gcgcccttgt agccgattcg agaaccaggg cggtcccgtg gggatcgatt gaccctttcg
     5161 ccgcttagct ctcttgcagg gtttcgccac cgacggcttt tcggctatag cccaggcgcg
     5221 ggccttcatg gcgggattcc tgaatgttgc ggttgaacgt ggtgggaaag agaataagtc
     5281 acggtgtcaa atgatgcgcg tcattcttgc acagcctcgg ggcttttgcg ccggtgtcgt
     5341 ccgcgcgatc gacatcgtgg agcatgcgct cgaaaaatat ggcgagcccg tctatgtgcg
     5401 ccacgaaatc gtgcataacc gtcacgtcgt cgagacgttg aagcgcaagg gcgcctgttt
     5461 cgtcgaggaa ctcgacgagg tgccgcacgg cgcagtgacc gttttcagcg cgcatggcgt
     5521 tccccaggca atcgtccgcg aagcggcgcg ccgcgaactt cctgtcctcg atgcaacatg
     5581 ccctctggtc tccaaagtgc atgctcaggg caaacgttat gtgtcgaaag gccgcacgct
     5641 cgtccttatc ggccatgcgg gccatcccga ggtggaggga acgatgggtc aggttggcgc
     5701 tcccgtccat ctcgtctctt ccgaagccga cgtgcaggct ctcgatcttc ctgacgatgc
     5761 gcccgtcgcc tatgtcacgc agaccacgct cagcattgac gacacgcgtg gcgtgatcgc
     5821 tgcactcaaa cggcggttta gcgacattgt agggccggat acatccgata tctgctacgc
     5881 gacgcagaac cgccagtcgg cggtacgcga attatgcaag atcgcagaag tcatcctggt
     5941 ggtgggcgcg aaaaacagct cgaattcgaa tcgtcttcgc gaaatcggac tcgaagaagg
     6001 cgttccaagt tatctgatcg ccgacggcag cgaactggat cccgcctggg ttgccggcaa
     6061 gctgacgatc gggcttaccg cgggcgcatc ggcccccgag gaactcgttc gcaacgtgat
     6121 cgacgcgttg cgcctgcttg gcgaagtcga agtcagcgaa atggacggcg ttgaggaaaa
     6181 gatcgaattc cgcctgcctg cagagttgag aaaccggaaa cgccaatcgg cgagtgtgga
     6241 gtaggacgtg ggaattccgc tgagacaggc tgttcgcgtc ggcgcctatg tgatgaagca
     6301 gcatctggcc ggacgaaggc gctatccgct cgtgctgatg ctcgagccgc tgttccgttg
     6361 caatctcgcc tgcgccggct gcggcaagat cgactatccc gacgagatcc tcaatcagcg
     6421 cctcagttac gacgaatgca tgcaggcgat cgacgagtgc ggcgctcccg tcgtctcgat
     6481 tgcgggcggc gaaccgctgc ttcaccgcga gttgcctgag atcgttcgtg gcttcctgga
     6541 gcgcaaaaaa ttcgtcatcc tgtgcaccaa cgctctcctg ctcgccaaga agatcgatca
     6601 gtttcgcccg catcgcgact tcacctggtc gatccatctc gacggcgaca aggcgatgca
     6661 cgacaagtcg gtctgcctcg aaggcacata cgaaaaagcc gtcgatgcga tcaggctggc
     6721 gaaatcgaaa ggcttcaggg tcagcatcaa ctgcaccctg ttcagcgatg cccagcccga
     6781 gcgggttgcc gccttcttcg acgaaatgaa ggcgatcggg ctcgatggca tgacgatttc
     6841 gcccggctat gcctatgagc gcgctccgga ccaggagcat tttctcaatc gtgaacggac
     6901 gaagaatctc ttccgtggga tcttttcacg cggcgatggc ggcagaaaat ggccgatcag
     6961 tcaatccgca atgttcctcg actttctcgc gggaaatcag acctatcgct gtacgccctg
     7021 gggcaatccc acccggaccg tctttggctg gcagaggccg tgctatcttc ttggcgaagg
     7081 ctacgcgccg acgtttcgcg agctgatgga ggaaaccgac tgggacaaat atggcgtcgg
     7141 caattacgag aaatgcgcga actgcatggt gcattgcgga tttgaagcga ccgccgtcat
     7201 cgacagtgta aagcgcccgt tggcggcact cggcgtgatg ctcaaaggcg tgaggaccga
     7261 aggtcagatg gcgcccgaga ttccgctcga caggcagcgg cccgcgcaat tcgtgttttc
     7321 atcccacgtc gagcgcaagc tcgccgagat tcgcaacgcc aaacccgcca acaagcaggt
     7381 ggcggcagcg gaatagcgcg gcgaacgcga tctccgcttc ccacgcggtg cgtagaagag
     7441 ccggaatttg ttgtggcgcg cgcaggagcg accgcatcac tcccacgatg tcgattccgc
     7501 ccgacggatg caacgcaacg caagctgccg gcggaagcgt gcggttagct gcatcggaga
     7561 ccacgcgaag cgccgcaaag ggcaggcctc tcgaccgtgc cgcgcgcgcc gccacatgcg
     7621 actccatgtc agccaccgat gcgcgggtcg ccgcgtacag tttcgccttg gcatcgcggt
     7681 ggcccgcgat cgtatctatg ccagccaggg gggccaccac cgcaccgggc acgcgcagcg
     7741 caagctcgcg cgtccacggc gcgtgggtgg gaatggattc cgagtcagcg acgacttcgg
     7801 atgcgacgat gacgtccccg actcgcagct cgggcgaaag cgcgccgcaa atgccgatgc
     7861 tcagtatgcg acgcgcgcgt tttccaagct catccgccaa acgccgctca agcgaaaccg
     7921 catcgccgcc gccaacgacc gacacgattt tgggatttgc gatcaggcgc gcctcgcgcg
     7981 cgagaccggt tacggcgaca atcattaaat tccgtatgaa acgcgattgg aattcccagc
     8041 ccggagattg cggtatcgcg ccaccgccca caacgggaag aacttcgaat acccatgata
     8101 gcgcaaatag aagacgcggg ggaagccgcc gcccgtgcac ccttcttctg gccaaagccc
     8161 ttgatcgtcc tgccttttct ggagaaagcc gatgccccgt gcgacagcgg ggtgatcgac
     8221 gcggcctgcg gccataagcc ccaggagcgc ccaggcggtc tgcgatggcg ccgaaggtgc
     8281 ggcctcgtat ccgcgataat cgagccggta gctgtcgcag ctctctcccc agccgccatc
     8341 gtcgttctgt atcgcgataa gccagtctgc tgcccggcga atggtgggcg aatggggatc
     8401 gaaccctgca gcattgaggc cagcgagcgc cgaccatgtg ccgtagatgt agttcacacc
     8461 ccagcgaccg aaccagctgc cgtctttttc ctggacgcgg gccatgaaat cgaccgccgc
     8521 cttcatggac ggactgtccg aagcctcgcc caattgggcg aacatgccga tgcaccgggc
     8581 ggtcacatcg gcggttggcg ggtcaagaag cgcaccgtga tcggcaaacg gaatgttgtt
     8641 gagatagtag taggtgttgt ccgcgtcgaa tgccgcccag ccgccattgt ggctctgcag
     8701 ccccgcaacc cattcacacc cgcgtgcgat cgcttcggag tagtcgctgt gcgccctgcc
     8761 gatgccgcgc gcgcgatcga gcgtcatcac caccacggcc gtatcgtcaa ggtcgggata
     8821 gtgagcgttg ttgtactgaa acgcccatcc acccggacgc gcatcgggcc gctcctcggc
     8881 ccagtctcct ttcacatgca aaacctggcg tgccttcagc cattcgcaca tggcgccgat
     8941 ccgcacatcg gcattcgcgc agcgggattc catcagggca tgcgcggcga gagcagtatc
     9001 ccacaccggc gagacgcagg gctggcaata ggtttcgtcc gcccggtgca cgaggagatt
     9061 ctcgaccgat ttgcgcgcca gcgcacgctc tgcatgatcc ggcggatagc cgagcgcgtc
     9121 atacatcatc acgctatttg ccatggcagg atagatggcg ccgagtccat cctcgccgtt
     9181 caatcgctcg gacacgaact ccatgcagcg cgcgatggca cgcttgcgca gtctggccgg
     9241 ccaaagcgcc tcgacaagct tcagaatgcg atcgagcacc gaaaacaaga ccgcccaata
     9301 ggggttctgg tgcggcgcgc gcaatggagg cggcgcgccg ctgccggcga acagctcccg
     9361 aatatggact ccgcgcggat tgcgcgccag cggccgcaga gtcgcaagga ccagcagcgg
     9421 cacaatcacc gtgcgcgccc aatacgacat tttcgacaga tggatcggaa accagcgcgg
     9481 cagcaggatg atctcgacgg gaatagtcgg cacgtttcgc caggacgatt ccccgaagag
     9541 cgcgagcagg atgcgcgtga aaacatttga tcgcgcggcg ccgccgcgcg ccagcagctc
     9601 ttcgcgcgct cggctcatat gcggcgcttc gggcagatcg ccgatcatct tcagcgcgaa
     9661 ataggccttg acagtcgcgc tgatgtcgaa ggcgccgcca tgataaagcg gccacccgcc
     9721 gtgatcggcc tggatgcggc gcaaatattt gccgacgcga agctcgagtt cggggttcgg
     9781 tgtctcgccg agatagtggg ccagcagcac atattccgcc gggatcgtgg
//
LOCUS       DASZMC010000605         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4919742,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000605 DASZMC010000000
VERSION     DASZMC010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4919742"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VG274_12290"
     CDS             complement(<1..851)
                     /locus_tag="VG274_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWE07484.1"
                     /translation="MTIDGRQVAGWLLSALAILAVLVLGRPLLAPLAFAVLIWAILNA
                     LTDALAHLRLPRMLAWTTSLLLIAAGLYLIARVLGNEADAVAAEAPVYLARLQRLALS
                     ALAFLHLGRGANFGDLLSGANVASMIGQVAASAGSFLFTLAMVIVYVGFLLAEQRHLP
                     GKLAKLQRNASRRDAAGEVIHAIARQVQAYLGVCTFLSAIMAAATFVLLTAMHVSFAG
                     FWALVLFIATYVPTIGAIGVLLPALMALLQYGSFAPFLIVSVALGALHFVLANIVSTV
                     MLGRTLNL"
     gene            complement(877..2088)
                     /locus_tag="VG274_12295"
     CDS             complement(877..2088)
                     /locus_tag="VG274_12295"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWE07485.1"
                     /translation="MNEERPTFVSHLECSATGERYEADQVHGRSRTGKPLLVRYALDR
                     VRDAVTKDALASRSRDLWRYRELLPVRHAHNIVSLGECMTPLIRIHAEGSEEILVKDE
                     GRLPTGSFKARGIALAVSMAKELGLSRLAMPTNGNAGAALAAYGSRAGMRTTVFCPED
                     TPEINVREIAAQGAKVYRVNGLIDQCGRLVGAGRDAAGWFDMSTLKEPYRIEGKKTMG
                     LELAEQLGWDVPDAIFYPTGGGTGLIGMWKAFAELEAIGFIGAKRPRMVAVQAAGCAP
                     IVRAFERKDTHAERWENARTIAAGIRVPSAIGDFLILAAVRESGGYAIAVDDDEIRKS
                     LEDVARHEGILLCPEGAATHAAWRKSLREGRIRAGDRVVLFNCASGLKYPLPPAEERL
                     DLNAPIDFARL"
     gene            complement(2085..2462)
                     /locus_tag="VG274_12300"
     CDS             complement(2085..2462)
                     /locus_tag="VG274_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HWE07486.1"
                     /translation="MAWKFPETIHLPQTLERHARIVEKGFWAKLLKLAGRIPFAEDLA
                     AAWFCAIDPTTPGRVRGVLLAALAWFVVPASIMPEFVVILGFTNEIAVTAIAVRMVRK
                     HLKERHYISARAALGICEPPPGQ"
     gene            complement(2623..3696)
                     /locus_tag="VG274_12305"
     CDS             complement(2623..3696)
                     /locus_tag="VG274_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07487.1"
                     /translation="MTKILMNSVALVALLAACAAPASAATLFQIVPVQGSKSTNVFGI
                     NKSQTITGSWLDSSGVEHGYVGPAATGSYTTFDDPTPGTEPRGLDNAGDVGGFGNSQS
                     GVASEYIPFERSAAGTIATVVNKRGKKLNYLIQGFSRNGVFAGSYVNGSNVDVAYLGK
                     NAMETKAVKLSLGNTGAAARGVDDAGDIVGWYLNTSGIQTGFLISGGVATSIVYPDAS
                     ESSTVMEGINNKGIATGQWTDTSAIVHSFTYNLATAKFKPVSIPGAVSFTQAWGINDL
                     GEIAVGSDAGFYIYCMKSICPGTGPAIAEGKEISVPKALMPSHSCADRACLTDVSQDP
                     VKVPARMRAPILKPGRKPGPMLP"
BASE COUNT          740 a         1254 c         1241 g          688 t
ORIGIN      
        1 agattgagtg tgcgcccgag catgacggtg ctcacaatgt tggcgagcac gaaatggagc
       61 gcacccagcg ctaccgaaac aatcagaaaa ggcgcgaacg agccgtattg caaaagcgcc
      121 atcagcgccg gcaacaggac gccgatcgcg ccgatcgttg gaacgtacgt cgcgatgaac
      181 aggacgagcg cccagaagcc ggcgaaactc acatgcatgg cggtgagcaa cacaaaagtg
      241 gccgccgcca tgatcgcgct cagaaaggtg cagacgccga gataggcctg cacctgccgg
      301 gcgatggcgt gaatgacctc ccccgccgca tcgcgccttg aagcgtttct ctgcagcttc
      361 gcaagttttc cgggcaggtg acgctgttcg gcgaggagaa agccgacata aacgatgacc
      421 atcgcaagcg tgaacaggaa cgatcccgcc gaggccgcga cctggccgat catgctcgcc
      481 acattggcgc cactgagcag atcgccgaaa ttcgcgccgc gtccgaggtg cagaaaggcg
      541 agcgccgaca gcgcaagccg ctgcagccgc gccaggtaga cgggcgcttc ggccgcaaca
      601 gcatcggcct cgtttccgag cacgcgcgcg atcaggtaga gcccggctgc gatcagcagg
      661 agcgatgtcg tccaggcgag catgcgcggc aaacgcaaat gcgccagagc atcggtgagc
      721 gcattgagaa ttgcccagat cagcacggca aatgccagag gcgcgaggag cggcctgcca
      781 agcaccagga ccgccaggat cgccagcgcg ctgagaagcc agcctgcaac ctggcgtccg
      841 tcgatcgtca ttgttcgtct tcctttccca aaaccctcac agcctggcaa agtcgatcgg
      901 tgcgttgagg tcgagtcgtt cctccgccgg cggcaaaggg tacttcaatc cgctggcgca
      961 gttgaaaagg acgacccggt cgccggccct gatgcggccc tcgcgtagcg attttcgcca
     1021 ggcggcatgg gtcgcggcgc cttccggaca gaggaggatg ccttcatggc gtgcgacgtc
     1081 ttcgaggctt ttccggattt cgtcgtcgtc gaccgcgatc gcgtagccgc cgctctcgcg
     1141 gacggccgca aggatgagga aatcgccgat cgcggacggc acgcggattc ccgccgcaat
     1201 cgtgcgcgca ttttcccagc gctcggcgtg cgtgtctttg cgttcgaagg cgcgcacgat
     1261 cggcgcgcag cccgcggcct gcacagccac catgcgcggg cgtttggcgc caatgaatcc
     1321 gatcgcctcg agttccgcga atgccttcca catcccgatc agcccggtgc cgccacccgt
     1381 tggatagaag atcgcgtcgg gcacatccca accaagctgt tcggcgagct cgaggcccat
     1441 cgtcttcttg ccctcgatgc gatagggctc cttcagcgtc gacatgtcga accagcccgc
     1501 tgcgtcgcgg cccgcgccga cgagcctgcc gcactggtcg atcagcccgt tgacgcgata
     1561 gactttcgcg ccttgcgcgg cgatctcgcg gacattgatt tcaggcgtat cctccggaca
     1621 gaacacggtc gtgcgcatgc cggcgcggct gccgtaagcg gccagtgcag cgcccgcgtt
     1681 gccgttggtc ggcatggcga gacgggagag ccccaattcc tttgccatcg aaacggcgag
     1741 agcgatgccg cgcgccttga aggagcctgt cggcaaccgg ccctcgtctt tcaccaggat
     1801 ttcttctgaa ccctctgcgt gaatgcggat aagaggcgtc atgcattcgc cgaggctgac
     1861 aatgttgtgc gcatgcctca ccggcagcag ttcgcgatag cgccacaaat cccgcgaccg
     1921 cgatgcgagc gcgtccttcg tcaccgcgtc tctgacccga tcgagcgcat agcgcacgag
     1981 cagcggcttg ccggtgcgcg agcgcccatg gacctgatcc gcctcgtatc tctcgccggt
     2041 ggcggagcat tcgagatggc tgacgaaggt ggggcgctct tcgttcattg tcccggcggc
     2101 ggctcgcaaa tgccgagcgc ggcgcgcgcg ctgatgtaat gccgctcctt cagatgcttg
     2161 cggaccatgc gcaccgcgat cgccgtcacc gcaatctcgt tggtgaagcc caggatgacc
     2221 acaaattccg gcatgatcga cgccggcacg acgaaccagg cgagcgccgc gagcagcacg
     2281 ccgcgaacgc ggccgggcgt tgtcggatcg atcgcgcaga accaggccgc cgccagatct
     2341 tcggcgaagg ggatgcggcc tgcgagtttc agaagctttg cccagaagcc cttttcgacg
     2401 atgcgcgcat ggcgctccag cgtttggggc agatggattg tctcgggaaa cttccatgcc
     2461 atgaggcaga atatcggacg cgggatgctt cgcggaaaga ggcaacgggt gaaccctgcc
     2521 cgttgccgcc catgtggccg cgtcgcatcg aaccgcgaag ccgcaaggaa aagccccctc
     2581 ctccgctgga ggagaaggcc aaagcgtggc gttccaggaa tctcagggga gcatggggcc
     2641 aggcttccgg cccggcttga gaatgggcgc gcgcatccgc gccggaactt tcacggggtc
     2701 ctgcgataca tcggtgagac aggcccggtc ggcgcaggaa tggctcggca tgagcgcctt
     2761 cggcactgaa atttccttgc cttcggcaat cgccgggccc gtgccggggc agatcgattt
     2821 catgcaatag atgtagaagc cggcgtcgga ccctacggcg atctcgccca gatcgttgat
     2881 tccccaagcc tgcgtgaacg acaccgctcc ggggatcgag accggtttga atttggcggt
     2941 cgcgagattg taggtgaagc tgtggacgat ggccgaggta tctgtccact gacccgtcgc
     3001 gatccccttg ttgttgattc cctccatgac cgtcgatgac tcggaggcgt cgggatagac
     3061 gatgctcgtg gccacgccac cgctgatcag gaatccggtc tggatgccgc tggtgttcag
     3121 gtaccagccg acgatatcgc ccgcatcgtc gaccccgcgt gccgcggcgc cagtgttgcc
     3181 gagagacaat ttgacggctt tcgtctccat ggcgttcttc ccaagatagg ccacgtcgac
     3241 attcgagcca ttcacgtagc tgcccgcgaa cacgccattt ctgctgaacc cttgaatcag
     3301 gtaattcagc tttttgcccc gcttgttcac aaccgtcgcg atcgtgcccg ctgccgaacg
     3361 ttcgaaggga atgtattcgg aagcgacgcc gctctgcgaa ttgccgaagc ccccgacgtc
     3421 gcctgcgttg tccaggccgc gcggttcggt ccccggcgtg gggtcgtcga aggtcgtgta
     3481 gctgccggtg gcggcgggcc ccacatagcc gtgttcgacg ccgctcgaat cgagccagct
     3541 gccggtaatt gtctggctct tgttgatacc gaacacgttg gtcgacttgc tcccctgcac
     3601 gggaacgatc tgaaacaggg tggcagccga agctggcgcc gcgcaggcgg ccaacagcgc
     3661 cacaagagcg accgaattca tcagaatttt ggtcatccct ccatctcccc ggactggcga
     3721 gatactaacg aacaggggag gggaaacttg tcaaccggca gcgcggttcc gccggcgctg
     3781 cgatatccgg tcggcggaaa tctgatggcc gtgtttctgc tttcccggca ggcgggaata
     3841 cctgcatagc gccgggcggt cgagatgccg ggcatctcgc ctcaaacggt gcgcgccaat
     3901 aaagaacccc ccatgccggt cag
//
LOCUS       DASZMC010000606         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2451989,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000606 DASZMC010000000
VERSION     DASZMC010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451989"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VG274_12310"
     CDS             <1..616
                     /locus_tag="VG274_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWE07488.1"
                     /translation="SRLIQQRPDLRAAEENLHSASAQIGVAVANRLPNLTLTGNVGST
                     APQIAQLFTSGAGNWTAAGSVLQPIFDAGTLYQKEVAARATFEQATSQYRSTVVTAFQ
                     NVADSLSALAHDAVALKKAAAAEEAANKSLTIARRRLELGDINYILLLNAQQTYFQAL
                     LSRVQAQASRFSDTVALFQALGGGWWNREDRDPPKPYPYFNFTN"
     gene            697..1521
                     /gene="panB"
                     /locus_tag="VG274_12315"
     CDS             697..1521
                     /gene="panB"
                     /locus_tag="VG274_12315"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWE07489.1"
                     /translation="MSVQSAIKRKTAPDIRARKNSEPIVMLTSYHAHTAALVDRYCDV
                     ILVGDSLGNVMHGFETTVPVTLEMMILQGRAVMRGSQNSLVVVDMPFGSYEASKEQAF
                     YSAARVLKETRCGAVKLEGGARMAGTIAFLVERGVPVMGHIGLTPQSINTLGSFRAQG
                     REEDTWEPIETDARAISEAGAFSVVVEAVAEPLARKITEAIMIPTIGIGASAACDGQV
                     LVLEDMLGLSVKAPKFVKRYGNLGPAIEAAIEGYATDVRSRNFPGPEHVYGMKTKT"
     gene            1658..>2050
                     /locus_tag="VG274_12320"
     CDS             1658..>2050
                     /locus_tag="VG274_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWE07490.1"
                     /translation="MQNPPLIARSLPALYRGLNKLRARDATIALVPTMGALHEGHISL
                     VRLAKRRAKKVVVSIFVNPTQFAPNEDLGSYPRTWIADIANLTKVGVDLVWIPDAPTM
                     YPQDFVTRVVVGGPATAGLEDRVRPHFFG"
BASE COUNT          394 a          635 c          621 g          400 t
ORIGIN      
        1 gtcccggctg attcagcagc gcccagacct gcgcgccgcc gaggaaaacc tgcattcggc
       61 cagcgcccag atcggtgtcg cggtcgcaaa ccgcctgccg aacctgacac tcacaggcaa
      121 tgtcggcagc acggcgccgc aaattgcgca gctgttcaca tcgggagccg gaaactggac
      181 ggccgccggc tcagtgcttc agccaatctt cgacgccgga acactctacc agaaggaggt
      241 ggccgcgcgc gcgaccttcg agcaggcgac gtcgcagtat cgcagcacgg tcgtaacggc
      301 attccagaat gtggccgaca gcctgagcgc gctggcgcac gatgccgtgg cgctgaagaa
      361 ggctgctgct gccgaagagg ccgccaacaa gagccttacc atcgcccgcc ggaggctaga
      421 acttggcgac atcaactata tcctgctcct aaacgcgcag cagacctact tccaagcgtt
      481 gctgtcgcgc gtccaggctc aggccagccg cttctctgat actgtggcgc tcttccaggc
      541 gctcggtgga ggttggtgga accgcgagga tcgcgatccg ccgaagccat acccatattt
      601 caatttcacc aattgacctc attccgtggt gcggcgtcca gatattcaac cagctctgtt
      661 ctcgctgacc gaggcggcaa ccattggagt ttaatcatgt ccgttcaatc tgccatcaag
      721 cgcaagacgg cgccggatat tcgcgcgcgc aagaattccg aaccaattgt aatgctgacg
      781 tcctatcacg cacacaccgc cgcgttggtc gatcgctatt gtgacgtcat tctggttggc
      841 gattccctgg gcaacgtgat gcatggcttc gagaccacgg tgccggtcac gttggagatg
      901 atgattttgc agggccgggc ggtgatgcgc ggttcgcaga actcgcttgt agttgtagac
      961 atgccgttcg gctcctacga ggcgtcgaaa gagcaggcgt tttattccgc ggcgcgagtt
     1021 cttaaggaga ctcgttgcgg tgcggtcaag ctcgaggggg gcgcccggat ggcggggacc
     1081 atcgcgtttc ttgtagaacg cggtgtgcca gtgatgggac atatcgggct tacgccgcaa
     1141 tcgatcaaca cgctaggctc gttccgcgcc cagggccgcg aggaggacac ttgggagccg
     1201 atcgagacgg acgcccgcgc catcagcgag gccggcgcat tctcggtggt ggtcgaggcg
     1261 gtggccgagc cgctggctcg caagatcacc gaagccatca tgatcccgac catcggcatc
     1321 ggcgcgagtg ccgcctgcga cggccaggtc ctggtgctgg aggatatgct tgggttgtcg
     1381 gtgaaggcgc cgaaattcgt caagcgctat ggcaatcttg ggccggcgat cgaggctgcg
     1441 atcgagggct acgccacgga cgtccgctcc cgcaattttc cggggccgga gcatgtctac
     1501 gggatgaaga cgaagacgtg atggccgagt agtccgtaat taaccggctc atcctcgcta
     1561 acgcgcgcca ccgtcgaaaa gctgtagtcg ttgatggcgg ttccacccat gagaatgccg
     1621 aaaacgcaat tgggtccctc tccacatccg aactcacatg cagaatccac ctctcatcgc
     1681 ccgctcgctc cccgccctct accgcggtct caataaactc cgtgcccgcg atgcgactat
     1741 cgcactggtg cccaccatgg gtgcgttgca tgagggccac atttcgctcg tccgtctcgc
     1801 caagcgtcga gcgaaaaagg tggtggtctc gatcttcgtt aatcccaccc aattcgcgcc
     1861 caacgaggat ctcggctctt acccgcgaac atggatagcg gacatcgcca acctcaccaa
     1921 agtcggcgtg gacctggtgt ggattcccga tgctccgacc atgtatccgc aggattttgt
     1981 cacccgcgtt gttgtcgggg ggcctgccac ggccggcctg gaagatcggg ttcggccgca
     2041 tttctttggc
//
LOCUS       DASZMC010000607         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1647305,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000607 DASZMC010000000
VERSION     DASZMC010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647305"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1449)
                     /gene="hflX"
                     /locus_tag="VG274_12325"
     CDS             complement(160..1449)
                     /gene="hflX"
                     /locus_tag="VG274_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWE07491.1"
                     /translation="MLLHVEPKRRGARTDRDAEARLAEAAGLTSAIGLSILAEHIVPL
                     TEARPASLIGKGKVEEIAAEARGLEPEVIVVNAALSPVQQRNLEKAWNAKVLDRTALI
                     LEIFGERARTREGRLQVELAHLTYQRSRLVRSWTHLERQRGGFGFLGGPGETQIETDR
                     RLIGERIAKIKHELEDVRRTRGLHRHARKRVPYPVIALVGYTNAGKSTLFNTLTKSAV
                     DARDLLFATLDPTMRGIRLPGGRRVVLSDTVGFIADLPVELIAAFRATLEEVLEADII
                     VHVRDVAHEESAAQKADVLRVLHELGIDTEGDARILELLNKIDLLEPAARAALRARNG
                     GEKAPVAVSALTGEGIPDFLTRLEIMLDREGLVLNLRLDPGDGAGMAWAYEHGRVLDR
                     RDRAKHVELSVAIDPDAKGRFEKRYGERMAIRMPRVN"
     gene            complement(1500..1751)
                     /gene="hfq"
                     /locus_tag="VG274_12330"
     CDS             complement(1500..1751)
                     /gene="hfq"
                     /locus_tag="VG274_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008204623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWE07492.1"
                     /translation="MTEKQQNLQDTFLNAVRKSKSSVTIFLVNGVKLQGNITWFDNFC
                     VLLRRDGHAQLVYKHAISTVMPLGPLQLQDAEPAALETA"
     gene            complement(2032..2922)
                     /locus_tag="VG274_12335"
     CDS             complement(2032..2922)
                     /locus_tag="VG274_12335"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF005209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HWE07493.1"
                     /translation="MWTDRGPVGRVAYVNGRYIRHDLAAVHIEDRGLQFADAIYEVFG
                     VFGGRIFDEEEHLDRLERSLGEIAMPMPMAREPLKLVLREMARRNHIVDGLIYLQVTR
                     GAVRRDHAVPSSSPRATLILTARLTDAESVERKRAGGVAVVTHSDQRWARCDIKSTGL
                     LANVLAKTAAREAGAYEAWLIDRDGHVTEGSSTTAWIVDRDGHLVTRDLDNAVLPGVT
                     RQVLTEVAADAQIRIVERRFTSDEALQAREAFLTAATVGALPIVQLDGKPIGDGRPGP
                     IARRLQDLYRHAASLKAGGE"
     gene            complement(2930..>3880)
                     /locus_tag="VG274_12340"
     CDS             complement(2930..>3880)
                     /locus_tag="VG274_12340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWE07494.1"
                     /translation="AISQLRQAIDKIAPTGSRVMITGPAGAGKELVARLIHAHSRRAG
                     HAFVAINCATMAPEKIESELFGEETSDGPRKIGLLELAHNGTLYLDEVADMPLETQGR
                     VLRVLVEQSFLRVGGATRVQVDARIVSSTTHDLRADIAVTQFREDLYHRLNVVPLRVP
                     SLCERRDDIPLLVTHFMKRLSVASGLMMREIGDDAMAILQSHGWPGNVRQLRNIVERL
                     LILASDDALQAISADLLPADFGNGGGWANGATGDLVISLPLREAREIFEREYLVAQIN
                     RFGGNISRTAAFIGMERSALHRKLKSLGVGSNGREPAFSA"
BASE COUNT          643 a         1274 c         1245 g          718 t
ORIGIN      
        1 cgccatcgcg ggctattacg cggacagcgg cggcgtgtac cacggcttcc tgcgaagccg
       61 gtaatgattt ccctcccccg caagtgcgcc agggcatcta cacatcacga gtgtgtagat
      121 accgttgcgc aagtgcgagg aaactttggt gcgtgctact taattcacgc gcggcatgcg
      181 tatcgccatc cgctcgccat accgcttctc gaatctccct ttcgcatccg gatcaatcgc
      241 gacgctcagc tccacatgct tcgcgcggtc gcggcggtcg agaacgcggc cgtgctcata
      301 ggcccaggcc attccggcgc catcgccggg atcgaggcgc agattcaaga ccagtccctc
      361 ccggtcgagc ataatctcga ggcgcgtgag aaaatcggga atgccctccc ccgtcagcgc
      421 tgacaccgcg acgggagctt tctctccgcc attgcgggcg cgcaacgccg cgcgggcggc
      481 cggttcgagc aggtcgatct tgttgagcag ttcgaggatg cgcgcgtccc cttccgtgtc
      541 gatgccgagt tcgtggagca cacgcagcac gtcggctttc tgcgccgccg attcctcgtg
      601 cgccacgtcg cgcacatgta cgataatgtc ggcttcgaga acttcttcga gcgtggcgcg
      661 gaatgcggcg atcagctcca ccggcagatc ggcgatgaag cccactgtgt cggagagcac
      721 gacgcgccgt ccgccgggca gtctgatgcc acgcatggtg ggatcgaggg tggcaaacag
      781 caggtcgcgc gcgtcgactg cggatttcgt cagcgtgttg aacaacgtcg acttgccggc
      841 attggtgtag ccgacaagcg cgattacggg atagggcacc cgcttccgcg catgccggtg
      901 caggccgcgg gtgcggcgca cgtcttccag ctcgtgcttg atcttggcga tgcgttcgcc
      961 gatcagccgg cggtcggtct cgatctgcgt ctcgccgggg ccgcccagga atccgaaccc
     1021 gccgcgctgg cgttcgagat gggtccagct gcgcaccagc cggctccgct gataggtgag
     1081 atgcgccagt tcgacctgca gccgcccttc tcgcgtgcgc gcccgctcgc cgaagatttc
     1141 gaggatcagc gcggtgcggt cgagaacctt cgcgttccac gccttttcga gattgcgctg
     1201 ctgcacgggg ctgagcgcgg cgttgacgac gatcacttct ggttcgagcc cgcgcgcttc
     1261 cgcggcgatc tcttccacct tgcccttgcc gatcaggctc gcgggcctgg cttcggtgag
     1321 cggcacgata tgttcggcga gaatcgagag cccgatggcg gaggtcagcc ccgccgcttc
     1381 ggccagccgc gcttccgcgt cgcgatccgt gcgcgcgcca cggcgtttcg gctcgacatg
     1441 cagaagcatg gcgctcgctt tttcgccgcc tcgcgatccg ccgcctcgcc gtttgggact
     1501 caggcggttt ccagcgccgc cggttcagcg tcctgaagct ggagcggccc gagcggcatc
     1561 accgtggaga tcgcgtgctt gtagacgagc tgcgcatgcc cgtcgcggcg cagcagcacg
     1621 cagaaattgt cgaaccaggt aatgtttccc tgaagcttga cgccgttgac cagaaagatg
     1681 gtcaccgacg atttgctctt gcgtacggcg ttgagaaaag tgtcttgcag attttgttgt
     1741 ttttcggtca tgggcaaacc ccactgtttg tctttattgt ccgcccctcg cggaacccgt
     1801 cagcataggt tcatttgctg cgcccgcgaa agcccaaata cacccctgca cccaatcgcc
     1861 tgtggtcaaa agctgaccct caagccacga agaaggaaga tttagcgcag ggtacgcaga
     1921 ggatgcgcag agggcgcaga gaaaacttgc gcgccggtgg cgcgctattc gtttcccccc
     1981 gcgtcctctg cgcatcctct gcgtcctctg cgctaaagcc ttttccctgc tctactcacc
     2041 gccggctttg agcgaggcgg cgtggcggta gaggtcttgc aggcggcggg cgatgggccc
     2101 gggccggcca tcgccgatcg gctttccgtc aagctgaacg atcggcagcg cgccgaccgt
     2161 cgccgcggtg aggaacgctt cccttgcctg tagggcttcg tccgacgtga aacggcgctc
     2221 gacgatccga atctgcgcgt ccgccgccac ttcggtgaga acctgcctgg tgactcccgg
     2281 cagcacggcg ttgtccaagt cgcgggtgac gaggtgcccg tcgcggtcga cgatccaggc
     2341 cgtcgtcgag gaaccttccg tcacgtgccc gtcgcggtcg atcagccagg cttcatacgc
     2401 gccggcctcg cgcgccgcgg tcttggcgag cacgttggca agcaggccgg tggatttgat
     2461 gtcgcagcgc gcccatcgct gatcggaatg cgtgacaacc gccacgccgc ctgccctttt
     2521 ccgctcgacg ctttcggcat cggtaagccg cgcggtgagg atgagcgtcg cgcgcgggct
     2581 cgacgaaggg accgcatgat cgcgccgcac cgcgccgcgc gtcacctgca gatagatcag
     2641 cccatcgacg atatggttgc gtcgcgccat ctcgcgcagc acgagcttga gcggctcacg
     2701 cgccatcggc atcggcatcg caatctcgcc aagcgagcgc tcgaggcggt cgagatgctc
     2761 ctcctcgtcg aaaatccgtc cgccgaagac gccgaaaact tcgtagatcg cgtccgcgaa
     2821 ttgcagcccg cggtcttcga tatgtacggc ggcgaggtcg tggcggatat agcggccgtt
     2881 gacataggca acgcggccga ccgggccgcg gtccgtccac atggcgccgt tacgcactga
     2941 aggcgggctc gcggccattc gatcccacgc cgagagattt cagcttgcgg tgcaaggccg
     3001 agcgctccat gccgatgaag gcggcggtgc gggagatatt gccgccgaaa cggttgatct
     3061 gcgcgaccag atattcgcgc tcgaagattt cgcgcgcttc gcgcagcggc agcgagatca
     3121 ccagatcgcc tgtcgcgccg ttcgcccagc cgccgccatt gccgaaatcg gcgggcagaa
     3181 ggtcggccga gatcgcttgc agcgcatcgt cgctcgccag gatcaggagc cgctccacga
     3241 tattgcgcag ctggcggaca ttgcctggcc agccatgcga ctggagaatc gccatcgcgt
     3301 cgtcgccgat ctcgcgcatc ataaggccgg aggctaccga caaccgcttc atgaaatggg
     3361 tgacgagaag ggggatgtca tcgcgccgct cgcacaggga tggaacgcgc aagggaacga
     3421 cgttcagccg atgatagaga tcctcgcgga attgcgtcac ggcgatgtcg gcgcgcagat
     3481 cgtgcgtggt cgaggacacg atgcgtgcat ccacctgcac gcgcgtggcg ccgcccacgc
     3541 gcaggaagct ctgctcgacc agcacgcgca gcaccctgcc ctgggtctcg agcggcatgt
     3601 cggccacttc gtcgaggtaa agcgtgccgt tatgggccag ctcgagcagc ccgatcttgc
     3661 gcgggccgtc ggaggtttcc tcgccgaaca attcggactc gattttttcc ggcgccatgg
     3721 ttgcgcaatt gatggcgacg aaagcgtgac ccgcccgccg cgaatgcgcg tgaatgagcc
     3781 gtgccacgag ttccttgccc gcacccgcag gccccgtaat catcacgcgg ctgccggtcg
     3841 gcgcgatctt gtcgatcgcc tgcctgagct gcgagatggc
//
LOCUS       DASZMC010000608         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_818120,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000608 DASZMC010000000
VERSION     DASZMC010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818120"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1637)
                     /locus_tag="VG274_12345"
     CDS             complement(21..1637)
                     /locus_tag="VG274_12345"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HWE07495.1"
                     /translation="MSQDTRHPGAHGLEQQGFRNLQAVNWNLPAPNLYERAVIRGEGH
                     IARGGALVALTGQHTGRSASDKFIVCDSTSDKQVWWDNNKPMTPAHFEALFDAMVAYA
                     QGRELYVQDLFGGADPTHRLPVRVVTELAWHSLFIRHLLIEPTAGERDAFVPEFTIID
                     LPGFHADPAIHGTETGTVIAVNFTRKLVLIGGTSYAGEMKKSVFTILNYLLPPRRVMP
                     MHCSANVGPNRDAAIFFGLSGTGKTTLSADATRTLIGDDEHGWSENGVFNFEGGCYAK
                     VIKLSPEAEPEIFATTRRFATVLENVMMDPVTRELDLDDGRYTENTRAAYPIDYIPNA
                     SRTGMAPHPKNVILLTADAFGVLPPIAKLTPSQTMYHFLSGFTAKVAGTEKGLGKEPQ
                     PTFSTCFGAPFMPRHPSEYGNLLRDLIAHHQADCWLVNTGWTGGAFGTGHRMPIKATR
                     ALLAAALDGTLANVEMRVDPHFRFRVPVSVPGVDPAILNPRETWKDKPAYDAQAIRLV
                     SMFRDNFRKFEAHVGADVLQAAPQLAEAAE"
BASE COUNT          300 a          539 c          575 g          325 t
ORIGIN      
        1 ctgcggcgcc attggcacgc ctactccgcg gcctccgcca gttgcggtgc ggcttgcagc
       61 acgtcggcgc cgacatgggc ttcgaatttg cggaaattgt cgcggaacat gctcacgagc
      121 ctgatcgcct gcgcgtcgta agcgggtttg tccttccagg tttcccgcgg attgaggatt
      181 gccggatcga cgccgggcac cgacacgggg acgcggaagc ggaaatgggg atcgacgcgc
      241 atctcgacgt tggcgagcgt gccgtcgagc gcagccgcaa gcaaagcccg agtcgccttg
      301 atcggcatgc ggtgaccggt gccgaaagcg ccgccggtcc acccggtgtt cacgagccag
      361 caatccgcct ggtggtgcgc gatcagatcg cgcagaagat tgccgtactc ggatgggtga
      421 cgcggcatga aaggcgcacc gaagcaggtc gagaaggtcg gctgcggctc tttgcccagt
      481 cccttttccg ttcccgcaac ctttgcggta aagcctgaca ggaaatgata catcgtctgc
      541 gacggcgtga gcttggcgat tgggggcagc acgccgaacg cgtctgccgt cagcaggatc
      601 acgtttttcg gatgcggcgc catgccggtg cgcgatgcgt tcgggatata gtcgatcgga
      661 taggcggcgc gcgtgttttc cgtgtagcgc ccatcgtcga gatcgagttc gcgcgtgacc
      721 ggatccatca tcacgttttc gagcactgtc gcgaagcggc gcgtcgtcgc aaagatttcg
      781 ggctcggctt cgggcgaaag cttgatcacc ttggcgtagc agccgccttc gaaattgaac
      841 acgccgttct ccgaccagcc gtgctcgtca tcgccaatca gcgtgcgcgt cgcatcggcg
      901 gagagtgtcg tcttgccggt cccggaaagg ccaaaaaaaa tcgcggcgtc gcggtttggg
      961 ccgacattgg ccgagcaatg catcggcatt acgcgcctgg gcggcagcag ataattgaga
     1021 atcgtgaaca ccgatttctt catctcgccc gcgtaggacg tgccgccgat caggacgagc
     1081 ttcctggtga agttcacggc gatgacggtt cccgtctccg tgccgtgaat ggccggatcg
     1141 gcgtggaagc ccggaagatc gatgatcgtg aactcgggca cgaaggcgtc gcgctctccg
     1201 gcggtcggct cgatcagaag atgccgtata aacagcgaat gccaggcgag ttccgtcacc
     1261 acgcggacgg gcagacgatg cgtgggatca gcgccgccga agagatcctg cacgtacagc
     1321 tcgcgccctt gcgcataggc gaccatcgcg tcaaagagcg cctcgaaatg cgcgggcgtc
     1381 atcggtttgt tgttgtccca ccacacctgc ttgtcgcttg tgctgtcgca gacgatgaac
     1441 ttgtcggacg ccgagcggcc cgtgtgctga ccggtgagcg cgacgagcgc accgcccctg
     1501 gcgatgtggc cttcgccccg aatgactgcg cgctcgtaga gattcggcgc gggcagattc
     1561 cagtttacgg cctgcagatt gcggaagccc tgttgctcga gcccatgggc gcccggatgc
     1621 cgagtgtcct gcgacatttc cctccactcc ccaactactc gcgggcccct ccaggccggc
     1681 ggagcgcggg cgttcggctg tccggccggc ccgccggggc aggataggct tccgccccg
//
LOCUS       DASZMC010000609         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1890300,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000609 DASZMC010000000
VERSION     DASZMC010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890300"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VG274_12350"
     CDS             <1..528
                     /locus_tag="VG274_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07496.1"
                     /translation="LDEPTNHLDMRAKDVLLDALKEYNGTVVFVSHDRYFIDNLAQRI
                     FEIEEGHVHVYPGNYEDYLWRKAGGPETMAAAQHAPLKAVEPEPATASEPKRDKPRVN
                     PMKAQKMRDRVKELEETIASLESEIQQQEAVLGEYKSAEETVRVSALLDQRRKDLEAR
                     VAEWEELTSELEASA"
     gene            complement(453..1643)
                     /locus_tag="VG274_12355"
     CDS             complement(453..1643)
                     /locus_tag="VG274_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWE07497.1"
                     /translation="MAVSSLTAETPRKGRIHKTTARKTPVVKVAAVKAPANPKPAVKR
                     KGIVRIMRGPWRVPTYADSTIGDRFEGEDIVVRHAAIDALAGMNGSVVVTHPDTGLVL
                     TIVNQQLAFKDGFQPCSTVKVPVALAALSESVVERNTLIHLYGRTSIGLTEALAKSNN
                     QYFATLGQKLGFERVSYYAKLFGLGEKAGYNIEGEEAGLLPRSIPHNGIGMMTSFGDG
                     ITVTPLELAALIGAVANGGSLYYLQYPRTLEEAAAIEPRMKRRLDIDAIIPEIKPGLA
                     GAVEFGTARRAGAASDETIYGKTGTCTDSRTPTHLGWFASFTDSPSGKIAVVVLLTGA
                     RAVSGPAASGVAGNVYKNLEAAGYFGAPRAANSSHEARYALASNSDVSSSHSATRASR
                     SLRR"
     gene            1864..>2681
                     /locus_tag="VG274_12360"
     CDS             1864..>2681
                     /locus_tag="VG274_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWE07498.1"
                     /translation="MSNPQIATKIPTQLIVRDSDGKSVLVPLDRDRITLGRSSACELA
                     YPDDSGLSRQHMAFVRDGGTWEVQDLGSKNGTLLNGKRLDQRTPFHMGDRVMAGHLTI
                     EFAGSDPAARPVSDKVVFVETPEANKSTTTVAANLESLIAPSGVDEINQGTIIQANPQ
                     MQALIRAGRELVGYRPLNELFEVIMDLAMQAVMAARGVLMTLEDGELVVRASRGAGFK
                     ISTTVRDRVLKERTSLLVRDTQLDQALREQKSIVQNKVRGMIAVPLQTNDRVIGL"
BASE COUNT          550 a          882 c          777 g          472 t
ORIGIN      
        1 ctggatgagc ccaccaacca cctggacatg cgcgccaagg acgtcctgct ggacgcgctg
       61 aaggaataca acggcaccgt cgtcttcgtc tcgcacgatc gctacttcat cgataacctg
      121 gcacaacgca tcttcgaaat cgaggagggc cacgtgcacg tctatccggg caattacgag
      181 gactacctgt ggcgcaaagc cggtggtccc gagacgatgg ccgccgccca acacgccccg
      241 ctcaaagccg tggagcccga acccgccacc gcgtccgagc ctaagcgcga taaaccccga
      301 gtaaatccga tgaaggcgca gaaaatgcga gaccgcgtga aggagctgga agagaccatc
      361 gcgtcgctgg aatcggaaat ccagcaacag gaagccgtgt tgggcgaata taagagcgcc
      421 gaagagaccg tgcgcgtaag cgcgctgctc gatcagcgcc gcaaggatct ggaagcgcgc
      481 gtcgcggagt gggaagagct tacgtcggag ttggaagcca gcgcatagcg tgcctcgtgg
      541 ctcgaatttg ccgcacgcgg agcgccgaag tatcccgccg cttccaggtt cttatacacg
      601 ttccctgcca cgccggacgc cgccgggccg ctcacggctc tcgcgcctgt cagcagcacc
      661 acgaccgcga tcttgccgct cggcgaatcc gtgaaggacg cgaaccagcc gagatgcgtc
      721 ggcgtgcgtg agtctgtgca cgtgccggtc ttgccgtaaa tggtttcgtc cgaagccgcg
      781 cccgcgcgcc gcgctgtgcc gaactcgacc gctcctgcga ggccgggctt aatttccgga
      841 atgatcgcgt caatatccag ccgccgcttc atgcgcggct cgatggcggc ggcttcctcc
      901 agcgtccgcg gatattgcag gtaatagaga ctgccaccgt tggccaccgc gccgatcaac
      961 gcggccagtt ccagcggagt caccgtgatg ccatctccga agctggtcat catgccgatg
     1021 ccgttgtgcg gaatgctgcg cggcagcaaa cccgcctcct cgccctcgat attgtagccg
     1081 gccttttcgc ccaggccgaa cagcttcgcg taataactga cgcgctcgaa tcccagcttc
     1141 tgtcccagtg tcgcaaagta ttgattgttg gatttcgcca gcgcctccgt cagcccgatc
     1201 gacgtgcgcc catacaaatg gattaaggtg ttgcgctcca cgaccgattc gctcaacgcc
     1261 gccagcgcca ccggcacctt cacggtggag cacggctgaa aaccgtcctt gaacgcgagc
     1321 tgttgattca cgatggtcag caccagcccc gtatcgggat gcgtcaccac cacggacccg
     1381 ttcattcccg ccaacgcgtc gatggccgcg tgccgaacca caatgtcttc gccttcgaag
     1441 cggtcgccga tggtggaatc cgcgtacgtc ggcacgcgcc atggcccgcg cattatacgc
     1501 acgatgcctt tgcgcttcac agcgggtttc ggattagccg gcgccttcac cgccgccacc
     1561 tttactaccg gtgtttttcg cgccgtggtc ttatggatcc ggccttttct aggcgtctca
     1621 gccgtcaggg aagaaacggc catggcggcc gtcacgacaa ttacgcagaa gtttcggaaa
     1681 tgcaaagacg aaaaacctcc tcggctcctg cgcgtcggaa aagcgcaagc agtataaaac
     1741 attgtcggcg agaaatggtt tctaggtcaa gccctaacca catcccgtcg tcgcatgact
     1801 ccgcgattca aaatgcgccg tcgtccgcgt ccggtacaac ggcaatgata acctaaaatc
     1861 ctggtgtcga atccgcagat cgcaacaaaa attcctaccc aactgatcgt ccgggatagc
     1921 gacggcaaga gtgttctggt gccgctcgac cgcgaccgga ttacgctggg ccgttccagc
     1981 gcctgcgagc tcgcttatcc cgacgattcc ggcctgtcgc gccagcacat ggccttcgtc
     2041 cgcgacggcg gcacatggga ggtgcaggac ctcggcagca agaacggcac gctgctgaat
     2101 ggcaagcgcc tggaccagcg caccccattt cacatggggg accgcgtcat ggccggccac
     2161 ctcaccatcg aattcgccgg ctccgatccc gccgcgcgcc ccgtgtccga caaggtcgtc
     2221 ttcgtcgaga cgccggaggc gaacaagagc accaccaccg tcgccgccaa tctcgaatcc
     2281 ctcatcgcgc cctcaggcgt tgacgagatc aaccagggca ccatcatcca ggcgaatccg
     2341 caaatgcagg ctctgatccg cgcgggacgc gagctggtgg gctaccgccc gctgaacgaa
     2401 ctattcgaag tgatcatgga tctggcgatg caagctgtga tggcggcgcg cggcgtgctc
     2461 atgacactgg aggatggcga gcttgtggtc cgcgcctccc gcggcgccgg cttcaaaatc
     2521 tccaccaccg tgcgcgaccg cgtgttgaag gaaagaacat cgctgctggt gcgcgacacg
     2581 cagctggatc aggcgctgcg cgaacaaaaa agcatcgtgc aaaacaaagt gcgcggtatg
     2641 atcgccgtgc ccttgcaaac caacgatcgc gtcatcggcc t
//
LOCUS       DASZMC010000610         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1079536,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000610 DASZMC010000000
VERSION     DASZMC010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079536"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1055
                     /locus_tag="VG274_12365"
     CDS             138..1055
                     /locus_tag="VG274_12365"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWE07499.1"
                     /translation="MTAPRKIAVLLGGRSAEREVSLVSGRACAEALRIEGFDCTEIDA
                     QGDVAAELCRASPDVAFNALHGRWGEDGCVQGLLELMRIPYTHSGVLASALAMHKQRA
                     KEVFRAEGLPVAGSVVVDRREAGTRHVMEPPYVVKPVNEGSSVGVFIIRKGDNRPPGE
                     LGSDTWALGDEVMVEEFVPGRELTVAVMGDRALAVTEIVSRTGFYDYEAKYAEGGSTH
                     VLPAPIPEEVSSDAMSIAVRAHRALGCRGVSRADFRFDDQRRRVVLLEVNTQPGMTPT
                     SLVPEQARHVGMSFCELVRWIVEDASCDR"
     gene            1043..1978
                     /locus_tag="VG274_12370"
     CDS             1043..1978
                     /locus_tag="VG274_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWE07500.1"
                     /translation="MRSLTAQARRGRASAAKPPRRGARSSAGARTATGSGRRRRPPNA
                     FVRAWRGLRPCFSMRHPAMWLSACVLFVILAAALLGGGYVTRAGNAARSAVRAVTADA
                     GFGIASIRISGNRRTDPRDVLAALGFGPGESIFAADMRTARAHLLSLEWVADAEVRRQ
                     YPDSISISIVEKMPFALWETPNGTYVVERSGRIIGLFDASAFPHLPVLAGEGAPANAA
                     ELVDAVAAHRALSARVAGYARISDRRWNLLLNDNVIVDLPEEGWTKELDVLEHLIVDR
                     GILERDISEIDLRMPDNFFFVLRNGQKQQMRGNAA"
     gene            1978..>2246
                     /locus_tag="VG274_12375"
     CDS             1978..>2246
                     /locus_tag="VG274_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07501.1"
                     /translation="MGETIRMRTSDAQSPAYRTGLVAALDVGSSKTVCLIGRAEPANL
                     RVIGAALRETRGIKSGTVTSLEYVEESIREAVAAAENLADLRVNSV"
BASE COUNT          391 a          702 c          748 g          405 t
ORIGIN      
        1 aagcattgta attttctcat caacaccggc gatgcgcggg cctccgacct cgaaggtctg
       61 ggcgaggaag tgcgcttacg cgtcgcctcc cgttccggca tcgatcttga gtgggaaatc
      121 aaacgcgtgg gcgtgtcatg accgcaccgc gaaaaatcgc cgtccttctt ggcggacgtt
      181 ctgcggaacg cgaagtgagt ctcgtttccg gccgcgcctg cgccgaggca ctgcgcattg
      241 aagggttcga ttgcacagag atcgatgccc aaggcgatgt tgcggcggaa ctttgtcgcg
      301 ccagccctga tgtcgccttc aatgccctgc atgggcgctg gggcgaagat ggatgcgtac
      361 agggcctgct tgaactaatg cggatcccct acacgcactc cggcgtgctc gcgtccgcgc
      421 tcgcgatgca caagcagcgc gcgaaagagg tttttcgggc cgaggggctg cccgtcgccg
      481 gcagcgtcgt ggtcgaccgg cgcgaggcgg gcacccgcca cgtgatggag ccgccctatg
      541 tcgtcaaacc ggtaaacgag ggatcgagcg tcggtgtctt catcatccgc aagggcgaca
      601 atcgaccgcc cggagagctt ggcagcgaca cctgggcgct tggcgacgaa gtgatggtgg
      661 aggagttcgt gcccgggcgc gagctgacgg tcgccgtgat gggcgacaga gctttggccg
      721 tgacggagat cgtctcgagg acaggtttct acgattatga agcaaaatat gcggagggcg
      781 gatccacaca tgtcctgccc gcaccgatcc cggaggaagt gagcagcgac gcgatgagca
      841 tcgcggtccg cgcacatcgc gcgctcggtt gccgtggcgt gagtcgcgcc gattttcgct
      901 tcgacgacca gcggcggcgc gtcgttcttc tcgaagtcaa cacgcagccg ggcatgacgc
      961 ccacttcgct cgtgcccgaa caggcccggc atgtgggcat gagtttctgc gaactcgtgc
     1021 gatggatcgt ggaggacgct tcatgcgatc gctgacggcg caggcgaggc gcggccgcgc
     1081 cagcgcggca aaaccaccgc ggcgaggcgc gcgatcgagc gccggtgcgc gcacggcgac
     1141 gggatccggg agacggcgcc gtccgccgaa tgctttcgtg cgcgcctggc ggggtcttcg
     1201 cccctgcttc tcgatgcgcc atcctgcaat gtggctctct gcctgcgttc tttttgtcat
     1261 tctcgccgcc gcactgctcg ggggcggata cgtcacccga gccggcaatg cggcgcgcag
     1321 cgccgtccgt gcggtcacgg ccgatgcggg cttcgggatt gcttccatcc gaatctcggg
     1381 gaatcgccgc accgatcccc gggacgttct cgccgcgctg ggcttcgggc cgggtgaatc
     1441 gatttttgcc gccgacatgc ggaccgcccg tgcgcatctg ctttccctcg aatgggtcgc
     1501 ggacgccgag gtgcgcaggc aataccccga ttccatctcc atctcgatcg tggagaaaat
     1561 gcccttcgcg ctctgggaaa ctccgaacgg cacgtatgtc gtggaacggt caggccggat
     1621 cataggcctg ttcgacgcga gcgcctttcc gcatctgccc gtactggcag gggagggcgc
     1681 gccggctaac gctgccgaac tggtcgatgc ggttgcggca catcgcgccc tcagcgcgcg
     1741 cgtcgcgggt tacgcgcgca tttccgaccg gcgctggaac ctgcttctca acgacaacgt
     1801 catcgtggac cttcccgaag agggctggac caaggagctc gatgtgctcg aacatctcat
     1861 cgtcgaccgg ggaattctcg agcgcgacat ttccgaaatc gacctgcgca tgccggacaa
     1921 tttctttttc gttctgcgca acggacaaaa acagcagatg cggggaaacg cggcataatg
     1981 ggcgaaacga ttcgcatgcg caccagcgac gcacaatctc cagcctaccg caccggcctt
     2041 gtggcggccc tcgatgtggg ttcgtcgaaa accgtctgcc tcatcgggcg ggcggaaccg
     2101 gcgaacctgc gggtgatcgg cgcggcgctg cgcgaaaccc gcgggatcaa atccgggacg
     2161 gtgaccagcc tcgaatatgt ggaggaatcg atccgcgagg ccgtggcggc ggcagaaaat
     2221 ctcgcagatc tgcgggtgaa cagcgt
//
LOCUS       DASZMC010000611         5398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_252279,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000611 DASZMC010000000
VERSION     DASZMC010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252279"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VG274_12380"
     CDS             <1..255
                     /locus_tag="VG274_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07502.1"
                     /translation="LIDAQLTARVYLELVGGRQARLRLAPRDAGRLTHADHAAARQRD
                     LELPCRLSATEAAAHTVFVAVELGGNPVWNWESGEGQLTG"
     gene            complement(240..734)
                     /gene="secB"
                     /locus_tag="VG274_12385"
     CDS             complement(240..734)
                     /gene="secB"
                     /locus_tag="VG274_12385"
                     /inference="COORDINATES: protein motif:HMM:NF004392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HWE07503.1"
                     /translation="MSDVGIGPNGNPPGGPEGPQIRIQIVGQYVKDLSFENPGAPMNL
                     TARPGIDLGVDLQARRLDGERFEVELKLRVSAKADDKPLFLLELAYGGLFLIQNAPEE
                     ILQQILLIDGPHLLFPFARRIVADAVRDGGMPPLMIEPIDFAALYRAKAGEAQQMRAG
                     QPVS"
     gene            937..1611
                     /locus_tag="VG274_12390"
     CDS             937..1611
                     /locus_tag="VG274_12390"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HWE07504.1"
                     /translation="MADSQLLGILLIAMVAGIILFRLYTILGRRTGNEREPQDRLRRV
                     AGAMPPVRADNVVALPDRASTPAEQQSGANDPVARGLLDIGLADRRFESERFLDGARH
                     AYEMIVTAFAARDRATLRPLLSDEVYAAFDAVMRGHEERKESVAYNFAGFKSVRITHA
                     ALKAPNAEITVEFSAQFTSTTTDGNGAVIEGDPKTVRDVTDIWSFARDTRAGDPNWTL
                     VATSGG"
     gene            1653..2864
                     /locus_tag="VG274_12395"
     CDS             1653..2864
                     /locus_tag="VG274_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HWE07505.1"
                     /translation="MLLVLLLTFSIAGTGVWILLRPPPSRSLTLVPLPFAAIRGWQTT
                     DARAALAAFQRSCSVILRFDPARPMGGAGYAGRAADWRHVCEAARRAGQGADGARKWF
                     EAWFSPFAAGSGYDREALFTGYFEPEIQVSREQHGSYTVPIYGPPEDLVTAELGDFRS
                     DLAGTRISGRVENRRLVPFPSRSEIEKQGLSDAPVLLYANSPVSLFFMQIQGSGRGRL
                     EDGTMLRLAYAAQNGRTYTPIGRTLIRRGALDRAHVSMHAIKAWLEAHPGAASQVMES
                     DDSYIFFRELPIGDSHLGSPGTEGVPLTPEASIAIDTGVHALGVPMFVDVAGSVDNPD
                     RPPMALACLCIAQDRGGAIKGNLRADVFWGFGAGAEAIAGSMKSSGLLYVLLPKTLAA
                     NLPSRFSGTRP"
     gene            2861..3409
                     /locus_tag="VG274_12400"
     CDS             2861..3409
                     /locus_tag="VG274_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWE07506.1"
                     /translation="MTSRKRPASEEERELFEAALSDALPLKSLPRRRRKHAAPATPLQ
                     APPPKLALPKKPLSRDGASGIDGRTADRLKRGLLEPQARLDLHGLDERSAHRALMTFL
                     RGAKARKIRLVLIVTGKGDARVPNGRPFDLGLDKRVRGVLREMTPRWLREPGLAELIA
                     DTRTAHRRHGGEGALYVYLRKG"
     gene            3416..3835
                     /locus_tag="VG274_12405"
     CDS             3416..3835
                     /locus_tag="VG274_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWE07507.1"
                     /translation="MKQEFAPIFISTHGPGFYSFGIEAGEFVQTTGIAEGLLTCFIRH
                     TSASLVIQENADPDVLADLEDFMRGLVPRETKAYRHTAEGPDDMPSHVRAALTTTSIS
                     IPVQRGKLALGAWQGLYLFEHRDAPHRREVLVHLIGE"
     gene            complement(3842..4621)
                     /locus_tag="VG274_12410"
     CDS             complement(3842..4621)
                     /locus_tag="VG274_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWE07508.1"
                     /translation="MELSASVSAIVTGGASGLGEATARALANHGVKIAIFDLQKEKGE
                     KIAREIRGVFCAVDVTKDDSVAVGFAAARAANGQERLLVNCAGTGNAVKTAARDRKTG
                     EIKFFAPAEFDRIVQINLVGTFRCIAHAAAGMLTLDPVGGERGVIVNTASIAAQDGQI
                     GQAAYAASKAGITGLTLVVARDLAREGIRCNTIMPGLFDTPLLQGAPDHVKQALGAQV
                     PFPPRLGNPVEYASLALEMIRNGYFNGECVRLDGAIRMAPR"
     gene            complement(4822..>5398)
                     /locus_tag="VG274_12415"
     CDS             complement(4822..>5398)
                     /locus_tag="VG274_12415"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWE07509.1"
                     /translation="ERRRYIRLDANLAGRLFVPGDGREAGCRIVDVSPVGAQVASEFV
                     PAMGTPVVLYIEGLGRFEAEVSRTQDDRFGVRFQCSALKRERVSEQLALLTERGGGNE
                     PVLRRHERTETPGFAHFTRANGEMVACEVLDLSLSGVSLKTETKPRIGETVVVGQMSG
                     RVVRHHESGIAIEFARAPAHKQDPAPMRNRL"
BASE COUNT          995 a         1701 c         1723 g          979 t
ORIGIN      
        1 ctcatcgacg cacagctcac ggcgcgcgtt tatctcgagc tcgtcggcgg ccgtcaggcg
       61 cggcttcggt tggcgccgcg ggacgctggc cgcctgacgc acgcagacca cgccgcggcg
      121 cgccagcgcg atctggagct gccgtgccgg ctcagcgcga cggaagccgc tgcgcacaca
      181 gtgttcgtgg cggtggaatt aggcggcaat ccggtttgga actgggagtc cggcgaaggt
      241 cagctcaccg gctgaccggc gcgcatttgc tgcgcctcgc ccgccttggc gcggtaaagg
      301 gctgcgaaat cgatcggctc gatcatcaga gggggcatgc cgccatcgcg caccgcatcg
      361 gcgacgatgc ggcgggcgaa cgggaaaagc agatgcggtc cgtcgatcag caggatctgc
      421 tgcaaaatct cttcgggcgc attctggatc agaaaaagcc cgccataggc caattcgagc
      481 aggaacagcg gcttgtcgtc ggctttggcg gacactctga gcttgagttc gacctcgaag
      541 cgttcgccgt cgagtcgccg agcctgaaga tccacgccga gatcgatccc cggccgcgca
      601 gtcagattca tgggtgcgcc aggattctcg aaagagaggt ccttcacata ctgtccgaca
      661 atctggattc gaatttgcgg cccttcgggg ccgcctggcg gattgccatt cggaccaatg
      721 cccacgtcgc tcatgaattg accgtcccgg aaaagcgggc ggggctatca tggataggcg
      781 ggactggcaa gccaaagcgg gtttgcactc gaaattgcgg cactcgaacc tatgttggga
      841 attaacatcc cgtagcggtt gtcgagatgg accccggcga tgccactggg gtagggtgcg
      901 tcggctcggc gggacatcgc aaagaggttt cgtgccatgg cagattcgca gctgcttgga
      961 attcttttga ttgccatggt ggccggcatc atcctgtttc gcttgtacac gatccttggg
     1021 cggcgaaccg gcaacgaacg tgagccccag gaccgacttc ggcgcgtcgc aggcgcaatg
     1081 cctccggtca gggccgacaa cgtcgttgca ctgccggatc gagcttccac tcccgcggaa
     1141 cagcaatccg gcgcgaacga cccggtagcg cgcgggctgc tggatatcgg gctcgccgat
     1201 cgccggttcg aaagcgaacg cttccttgat ggtgcacggc atgcctacga gatgattgtc
     1261 accgcctttg ccgcacgaga tcgcgccacg ctccgtccgc ttctcagtga cgaagtatac
     1321 gcggccttcg acgccgtcat gcgcggacac gaggagcgca aggaatcggt ggcttataac
     1381 ttcgcggggt tcaaaagcgt tcggatcacg catgcggcgc tcaaggcgcc aaacgcagag
     1441 atcacggtgg aattctccgc ccaattcact tcgaccacga cggatgggaa cggcgcggtg
     1501 attgaaggcg atcccaaaac cgtgcgcgat gtgacagaca tctggagttt cgcgcgcgac
     1561 acgcgggcag gcgatcccaa ttggacgctc gtggcaacga gcggcggctg aaagcgcatc
     1621 gcgcccggct gcccgggcgg cgccgggctg gcttgctcct cgttcttctg ctgacgttct
     1681 cgatcgccgg caccggtgtg tggatcctgt tgcgaccccc accctctcga tcgctgacgc
     1741 tcgtgcctct gcctttcgcc gccattcggg gctggcagac gaccgacgcg cgagccgcgc
     1801 tcgcggcctt ccagcggtcc tgttcggtca ttctgcggtt cgaccccgcc cggccgatgg
     1861 gcggcgcggg ttacgcgggc cgtgcggccg actggcgaca tgtgtgcgag gcggcgaggc
     1921 gggccgggca gggcgcggat ggcgcgcgga aatggttcga agcgtggttt tcgccgttcg
     1981 ctgccggatc gggatacgat cgggaggctc ttttcaccgg atatttcgaa ccggagattc
     2041 aggtgagccg ggagcagcac ggctcttaca cggtgccgat ctatggacct cctgaagatc
     2101 tcgtcacggc cgagctgggg gatttccgaa gcgatctggc aggaacgcgc atctcgggcc
     2161 gagtggaaaa ccgccggctg gtgccgttcc cctcgcggtc cgagatcgaa aagcaggggc
     2221 tcagtgatgc gcccgtcctg ctctatgcaa acagtccggt ttcgctcttc ttcatgcaga
     2281 tccagggttc ggggcgcggc cggctcgaag atggaaccat gctgcgactg gcatatgcgg
     2341 cgcaaaacgg ccgcacctat acaccgatag gccgcacgct catccggcgg ggcgccctcg
     2401 atcgcgcaca tgtttccatg cacgccataa aggcctggct cgaggcgcac cccggtgcgg
     2461 cgagccaggt catggagagc gacgattcgt atattttctt tcgagagctg cccatcggtg
     2521 attcacacct gggaagcccg gggaccgaag gtgtgccact gacaccggaa gcgagcattg
     2581 cgatcgatac gggcgtgcac gcgctcggtg ttccaatgtt tgtggacgtg gcgggatccg
     2641 tcgacaatcc ggatcgcccg ccaatggccc ttgcgtgtct ctgtatcgca caggacaggg
     2701 gaggcgccat caagggcaat ttgcgtgccg acgttttttg ggggtttggg gccggggctg
     2761 aagcaatcgc cggcagcatg aaatcctcgg ggctcctcta tgtcctgttg ccgaaaacac
     2821 tcgcggcaaa tcttccgtct cgattttcgg gaacgcggcc gtgacctcac gcaagcgacc
     2881 cgcgagcgaa gaagagcgcg aactctttga agccgcgctc agcgacgccc tgccgttgaa
     2941 atccctcccg cgccggcgga ggaagcacgc cgctcccgca actcctcttc aggcgccgcc
     3001 accgaaattg gctttgccaa aaaagccgct atctcgggac ggcgcatccg gcatcgacgg
     3061 caggaccgcc gatagattga agcggggtct gctggagccg caggcccggc ttgatctcca
     3121 cggtctggat gagcgctcgg cccaccgggc tctgatgaca ttcctgcgtg gcgccaaggc
     3181 gcgcaagata cggctggttc tcatcgtaac gggcaaaggc gacgcgcgcg tgcccaacgg
     3241 ccggccgttc gatctcggtc tcgacaaacg cgtgcgcggc gtgttgcgcg agatgacgcc
     3301 gcgatggctg cgagagcccg ggctcgcaga actgatcgcc gatacgcgca cggcgcaccg
     3361 gcggcatggc ggcgagggcg ctctctatgt ttatctccgg aaaggatgag gccgcgtgaa
     3421 gcaggaattt gctccgatct tcatctcaac ccacggcccc ggcttctatt ccttcggcat
     3481 cgaagcggga gagttcgtcc aaaccaccgg aatcgcagag gggctcctga cctgctttat
     3541 ccggcacacc tccgcgtcgc ttgtcattca ggagaacgca gatccggacg tgcttgccga
     3601 tctggaagac ttcatgcgcg ggctcgttcc gcgcgaaacg aaagcctaca gacacaccgc
     3661 cgaaggtccc gacgacatgc cgagccacgt ccgggcggca ctgactacga catcaatttc
     3721 gataccggtt cagcgcggca agctcgcgtt gggcgcctgg cagggcctct atctcttcga
     3781 gcaccgcgac gctccccatc gccgggaggt tctcgtacat ctgatcggag aatgaacgat
     3841 ttcaacgcgg ggccatccga atggcgccgt cgaggcgcac gcattcaccg ttaaagtagc
     3901 cgttgcggat catctcgagt gcaagcgagg catactcgac cggattgccg agtcgcggcg
     3961 ggaagggcac ctgtgcgccc aaagcctgct tcacatgatc gggcgcgccc tgcagcagag
     4021 gagtgtcgaa aagccccggc atgatcgtgt tgcagcgaat gccttcccgc gccaggtcgc
     4081 gagcgaccac cagcgttagg ccggttatcc cagctttgga ggccgcgtaa gccgcctggc
     4141 cgatctgacc atcctgcgct gcgatcgaag ccgtattgac aatcacgccc cgctcgcccc
     4201 cgacaggatc aagcgtcagc attccggcag ccgcgtgcgc aatgcagcga aacgtgccga
     4261 cgaggttgat ctggacgatg cgatcgaatt ccgcgggggc aaagaacttg atctcgcccg
     4321 tcttgcggtc gcgcgcggct gtcttgaccg catttcccgt accggcgcaa ttgacgagga
     4381 ggcgctcctg gccgtttgcg gcacgagcag ccgcgaaacc cacggcgaca ctgtcatctt
     4441 tggtcacgtc gaccgcgcag aagacgccgc gaatttcgcg cgcgattttt tcaccctttt
     4501 ctttctgcag atcgaaaatg gcgatcttga ctccatgatt ggccagagcg cgggctgtgg
     4561 cctcgccgag acccgacgcg ccgcccgtca cgattgccga taccgatgca ctgagctcca
     4621 tgacaccctc gagacatttc cgcagccggc agtcttaggg caaacggccg atgaattgcc
     4681 agaagtgggc gcgcggaatg cgcggaacag cgattgcgca cgatccacta cgagtctgct
     4741 gaatccgccg gagtgcggcc ggacgttcgc cgggttcgtt ccgagcaatg aaggcaattc
     4801 gattttcggg cgcgaggcgg ctcagagccg gttgcgcatc ggagcaggat cctgcttgtg
     4861 tgccggagcc cttgcgaact cgatggcgat tccactttcg tgatgacgga ctacacggcc
     4921 cgacatctgt ccgacaacga cggtttcgcc aatgcgcggc ttcgtttccg tcttgagtga
     4981 aacgccgctg agagaaagat cgagcacctc gcatgcgacc atctcgccat tggcgcgggt
     5041 gaaatgagcg aacccggggg tctccgtgcg ttcatgccgc cgcaggacag gttcgtttcc
     5101 cccgccgcgc tcggtcagca gagcaagctg ctctgaaacg cgctcccgct tcagcgccga
     5161 gcattgaaag cgcacaccga aacgatcgtc ctgggtccga gagacttcgg cttcaaagcg
     5221 cccgagccct tcgatataga gcacaaccgg cgtgcccatc gccgggacga attcggaggc
     5281 gacttgcgct ccgacgggcg acacgtcgac gatccggcaa cccgcctcgc gaccgtctcc
     5341 cggcacaaac aggcgaccgg cgagatttgc atccagccgg atatagcgcc ggcgctcg
//
LOCUS       DASZMC010000612         4920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3806741,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000612 DASZMC010000000
VERSION     DASZMC010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3806741"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1032
                     /gene="lexA"
                     /locus_tag="VG274_12420"
     CDS             286..1032
                     /gene="lexA"
                     /locus_tag="VG274_12420"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWE07510.1"
                     /translation="MLTKKQYELLMFIHERVRESGVPPSFDEMKDALDLKSKSGIHRL
                     ITALEERGFLRRIEKRARALEIIKLPDNIADTVRPATTRSQVQRMNPAGARHGRSEVR
                     GSSDARVAGSPRRTGGESEGSVNVPMVGRIAAGTPIEALQNRISDVPVPMGMIGRGDH
                     YALEVTGDSMINAGILDGDTVIIQESDTANSGEIIVALVDESEATLKRLRRRGDSIAL
                     EAANPAYETRLYGADRVRVQGKLVGLIRRY"
     gene            complement(996..3188)
                     /locus_tag="VG274_12425"
     CDS             complement(996..3188)
                     /locus_tag="VG274_12425"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWE07511.1"
                     /translation="MIGPLTSFRRHAPDVADIGKWPLAMVRVLRIHIFAERERWPLWL
                     PVGFGTGIGLYFGLSVEPSITLAALLGVIGLACAILSARSFHVALNVALAAIAALSLG
                     FAYAKWRTETVAAPVLSHKVGPVGLDGRIESVQIHGKGVRVVLSAIRSKRFHEGNTPA
                     RVRVSIRAASSDIVPGNWIHVMAVLMPPPGPAAPGGYDFGRAAFFDRIGGVGYAYGRA
                     TTIAPLRPESLRERIATSIAQLRWRMTQRIQAALPGSTGGIASALITGDRGSISDDDE
                     AALRDAGLAHVLAIAGLHMALVGLGLFWVVRAVLAALPFVALNYPIKKWAALAALGGA
                     TFYLIVSGAATPATRAYIMLATMLTAILMDRPALSMRSVALASLTLLLLRPESLIEPG
                     FQMSFAAVVGLVAVAEWEAAHRNQDGPRAFQSVRRYVWGIAVTSFVGSIATAPFAIFH
                     FDRATHYAVLGNLLAMPVMGFVAMPAAAISVIAMPFHLEAWPLKVMGFGIGIMLSVGR
                     FVSGLPGAISTVSAWPVGALVLMAFGGLWTALWRRNWRWLGLVPVAAGIVLALTTHGP
                     DLLVARDASTVAVRGKDGVLRLMRKASDGYSADQWLKRDGDGRDADAAVATRKDGVSC
                     DGYGCIAKGRDGTVIAATTRIDALEEDCAAATIVVSALPAGRDCIQPKLVIDKFTIAR
                     AGGYAVWLGNTLRVETVEGERGQRPWSLQPRAPRTPNQYRRIRPTNLP"
     gene            3256..4668
                     /gene="gltX"
                     /locus_tag="VG274_12430"
     CDS             3256..4668
                     /gene="gltX"
                     /locus_tag="VG274_12430"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWE07512.1"
                     /translation="MNGNKKPVLRFAPSPTGMLHIGGARTALFNWLYARHTGGTFLLR
                     IEDTDRERSTPEAVAAILKGLEWLGLDWDGQTVYQFARAGRHREVAEQLLSEGKAYRC
                     FATAQELDEMRAQQRSAGKSMRYDGRWRDRNPSEAPAGAPFVVRLKAPQTGETIVHDV
                     VQGDVRFQNEQLDDMVLLRSDGTPTYMLAVVVDDFDMNVTHVIRGDDHLNNAARQLQI
                     IHAMGWPEPVYGHLPLIHGPDGAKLSKRHGALAVEAYRDMGYLAETMRNYLLRLGWSH
                     GDDEIIPTEKAIEWFNLESIGRSPARLDFKKLDNLNAHYIRAMDNDSLVKEIEAVLAR
                     ETPPRTLDEKSRTRLTAAMPGLRERAKTLLELAQAADFLFSDGPRTLDDAAQKNLPAE
                     ARVTIGKALPSLEATDWTGKVLEAAARAFADAEGLKLGQIAQPLRAALTGKASSPPLF
                     EMMEVLGRQESLVRLKAYAG"
BASE COUNT         1020 a         1572 c         1499 g          829 t
ORIGIN      
        1 gcgccgcatg tcccggaagg cacgctcgtc gtcgccgaaa gcggactgtc gactccggct
       61 gatctgaagc gcctcgacga cgcagacgtg accaccttcc tgatcggcga aagcctgatg
      121 cgccaagccg atgtggaagc cgcaacgcgc gcactgatcg gcgcaaagct ctagggctcg
      181 cccaacccaa gattttgtac gttgacgggt tttgagaact aaagtagaac gattctccat
      241 gtttcggatt tgttcgccac atggcgccta gggagatatg gaaccgtgct aaccaagaag
      301 cagtacgaac tcctcatgtt catccacgaa cgggtgcgtg agagcggcgt gccgccctct
      361 ttcgacgaaa tgaaggatgc gctggacctc aagtccaagt caggcatcca tcgtctgatc
      421 acagcattgg aggaacgcgg gttccttcgc cgcatcgaaa agcgcgcgcg tgcgctcgaa
      481 atcattaagc tcccggacaa tatcgccgac acggtccgtc cggcgacgac gcgcagccag
      541 gtgcagcgta tgaacccggc cggcgcgcgg catggccgct cggaggtacg cggctcgtcc
      601 gatgcccgcg tggcaggcag tccgcggcgc acgggtggcg agagcgaagg ttccgtgaac
      661 gtgccgatgg taggccgcat cgcggcgggt acaccgatcg aagcccttca gaacaggatt
      721 tccgacgtgc cggtgcccat gggcatgatc ggccgcggcg atcactacgc gctcgaagtc
      781 accggtgatt ccatgatcaa cgctggaatc ctcgacggcg acaccgtcat cattcaggaa
      841 tccgacacgg ccaacagcgg cgagatcatc gtcgccctcg tcgatgaatc ggaggcgact
      901 ttgaagcggc tgcgccgtcg cggcgactcc attgcgctcg aagcggccaa tccagcctac
      961 gagacacgcc tctacggcgc ggaccgcgtg cgtgttcagg gcaaattggt cggtcttatt
     1021 cgccggtact gatttggtgt tcgaggcgcg cgcggttgca ggctccaggg cctttgaccg
     1081 cgttcgcctt cgacggtctc cactcgcagc gtgttgccga gccaaaccgc ataaccgccg
     1141 gcgcgcgcga ttgtgaattt gtcgatgacg agtttgggct ggatgcaatc gcgtccggcc
     1201 ggaagggcgc ttaccacgat ggttgctgcc gcacagtctt cctcaagcgc atcgatgcga
     1261 gttgtggccg cgatcacggt gccgtccctg cccttcgcga tacagccata gccatcgcaa
     1321 ctgacgccgt ccttgcgtgt cgcgaccgcg gcatcggcgt cgcgcccgtc accgtcccgc
     1381 ttcagccatt gatcggctga ataaccgtcg ctcgccttgc gcatcagacg cagaacgccg
     1441 tccttgcccc gcaccgcgac tgtcgacgca tcgcgcgcaa ccaacaggtc gggcccgtgc
     1501 gtcgtgagcg cgaggacaat gccagcggcg acaggaacga ggccaagcca tcgccagttt
     1561 cgccgccaga gcgccgtcca caatccgccg aatgccatca gaacgagtgc accaaccggc
     1621 cacgccgaca cggtcgagat tgcacccgga aggcccgaaa cgaaccgccc caccgacagc
     1681 atgatcccga ttccgaagcc catcactttc agcggccatg cttcgagatg aaaaggcatc
     1741 gcgatcaccg agatcgcggc agcaggcatg gcgacaaacc ccatcactgg catcgcaagc
     1801 agattgccaa gcactgcata gtgggtggcg cgatcgaaat ggaatatggc gaacggcgcg
     1861 gtcgcgatgc tgccgacaaa gctcgtgacg gcgatccccc acacatagcg gcggacgctc
     1921 tggaaggcac ggggtccatc ctggttgcga tgcgccgctt cccattccgc gacagcgacg
     1981 agtccgacga cggcggcgaa ggacatctga aatcccggtt cgatcaggct ttcgggcctc
     2041 agaagcagaa gggtcagcga ggccagcgcc acggaccgca tcgacagtgc gggacgatcc
     2101 atcaggatcg cggtcagcat cgtcgccagc atgatgtatg cccgcgtcgc cggcgtcgcg
     2161 gcaccgctga cgatcagata aaatgtcgcg ccgccgagtg ccgcgagcgc tgcccacttt
     2221 ttaattggat aattcagcgc gacgaaagga agggccgcga gtaccgcgcg cacgacccag
     2281 aacaatccga gtcccaccaa cgccatatgc aggcccgcga tagcgagaac atgggccaat
     2341 cccgcatcgc gcaatgccgc ctcgtcgtcg tcggagatcg aaccgcggtc gccggtgatc
     2401 agcgcagagg cgatcccgcc tgtgctgccg ggaagtgccg cctgaatgcg ctgcgtcatg
     2461 cgccagcgca attgcgcgat ggatgttgcg attcgctctc gaagactctc gggccgcagc
     2521 ggcgcaatgg tggtggcacg gccatacgca tagccgacgc caccgatgcg gtcgaagaat
     2581 gcggcgcgtc cgaaatcgta tccacccggc gcggccggtc cgggtggcgg catcagtacc
     2641 gccattacat gaatccagtt gcctggcacg atatccgacg aagcggcgcg gatcgaaacg
     2701 cgcacacgtg ccggcgtatt accttcatga aatcgtttcg agcgaattgc ggacagaacg
     2761 acccgcacgc ccttgccatg gatctgtacg ctctcgatgc ggccatccag gccaacagga
     2821 ccgactttgt gcgacaggac cggcgcagcg acggtttccg tgcgccattt ggcataggcg
     2881 aagccgagcg agagcgcggc gattgcagcg agcgcgacgt tgagtgccac gtggaaagac
     2941 cgtgccgaca ggattgcgca agcaagacct ataacgccga ggagcgcggc cagcgtgatc
     3001 gatggctcga cggacagtcc gaaataaaga ccgatgccgg ttccgaaacc gaccggcagc
     3061 cacagaggcc agcgttcgcg ttcggcgaag atgtgtatgc gaaggacgcg caccatcgcc
     3121 aacggccact tgccgatgtc tgcgacatcg ggcgcgtggc gccggaacga cgtcaacgga
     3181 cctatcacca tggaaaggcg cctgctagaa gcctcgacag aaaccacagc cacgaacgcg
     3241 gcccgttttt ctaccatgaa tggaaataag aaacctgtcc ttcgtttcgc gccctcgccc
     3301 actgggatgc tccacatcgg cggcgcgcgc acggccttgt tcaactggct ttacgcccgc
     3361 cacaccggcg gcacgttcct gctgcgcatc gaggacaccg accgcgaacg ctcgacgccc
     3421 gaggctgtcg ccgcgatcct gaaggggttg gagtggctgg gtctcgattg ggatgggcag
     3481 accgtctacc agttcgcgcg cgctggccgg caccgcgagg tcgccgagca gttgctctcc
     3541 gaaggcaagg cctatcgctg tttcgcgacc gctcaggaac tggacgagat gcgcgcgcag
     3601 cagcgctcgg caggcaagtc gatgcgctat gacggacgct ggcgcgaccg caatccatcg
     3661 gaggcgccgg ccggggcgcc gttcgtcgtc cggctcaagg cgccgcagac cggcgagacc
     3721 atcgtgcacg atgtcgtgca gggcgatgtg cgcttccaga acgagcagct cgacgacatg
     3781 gtgttgctgc gcagcgacgg cacgccgacc tacatgctcg ccgtcgtcgt cgacgatttc
     3841 gatatgaacg tgacgcacgt catccgtggc gacgatcatc tgaacaacgc cgcgcgccaa
     3901 ctccagatca tccatgccat gggatggcca gagcccgttt atggccacct cccgctgatc
     3961 cacggtcccg acggagcgaa gctgtcgaag cgccacggcg cgctcgcggt cgaggcttat
     4021 cgtgacatgg gctatctcgc cgaaacgatg cgcaattatc tcctccgcct tggctggagt
     4081 cacggcgacg atgaaatcat cccgaccgag aaagcgatcg agtggtttaa tctcgaaagc
     4141 atcggccgta gccctgcccg tctcgatttc aagaagctcg acaatctcaa cgcccactac
     4201 atccgtgcga tggacaacga ttcgttggtc aaagagatcg aagccgtact cgcgcgtgaa
     4261 acgccgccac gcacgctgga cgaaaaatcc cgcacgcgtt tgaccgccgc gatgccggga
     4321 ttgagggagc gtgccaagac actgctcgaa cttgcgcagg ctgcggactt cctgttctcc
     4381 gacggtccgc gcaccctcga cgacgcagcg cagaaaaacc tcccggcgga agcgcgtgtc
     4441 acgatcggca aagcactgcc ctccctcgag gccacggact ggaccggcaa ggtgctcgag
     4501 gccgccgccc gcgccttcgc agatgcggaa ggactcaaac tcgggcagat tgcccagccc
     4561 ctgcgcgccg ccctaaccgg caaggcttct tcgcccccct tatttgagat gatggaagtg
     4621 ctgggaaggc aggaatctct tgtccgatta aaggcttatg cggggtagcc taactcatct
     4681 caaattgtcc tgattttcag gaatgtgatg acgaaattaa ccgagttggc ttataattgt
     4741 gcagtgcaag atatttgaat cgagcgctcc ccggagggag ccagacagga gccagagatg
     4801 aaggccagca agcttgtaga tgcgggtaag gccacgctga tctacgaaaa caagtctttc
     4861 gaactgcctg ccttctccgg ctccgaaggc cccaacgttc tcgacatccg caagctctat
//
LOCUS       DASZMC010000613         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1082721,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000613 DASZMC010000000
VERSION     DASZMC010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082721"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VG274_12435"
     CDS             <1..745
                     /locus_tag="VG274_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07513.1"
                     /translation="APPPPPAPRHHHVHVAQSATPDAPPAPPASPAPEAMSAPPAPPP
                     PPAMHAVPPVPSVPAIALNEDDEDVNVDVDNDDDTVSVKGDKVVKMRVWRDKEGHRHV
                     IRSERFLSPADKARIHEEVARAQVEARRAEVEIARVQPEVDKALAAAKINEKVAKAMR
                     EAEPRIRAEIDRAMAEAKPEIRRAMAEAHINEKVMKALRDAQPKIDAAIAKAHDKEWH
                     ARVMQEERDDASDKYDDNDNDDSQDQNAH"
     gene            complement(809..1117)
                     /locus_tag="VG274_12440"
     CDS             complement(809..1117)
                     /locus_tag="VG274_12440"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HWE07514.1"
                     /translation="MNVVCHIRYEIDPFKRAAFEAYAKAWLTLIPACGGALIGYFLPL
                     EGTNYEAHAMIGFESLAAYENYRACLRNDPKSVANFEFAQREKFIVREERRFLQPVKA
                     "
     gene            1192..1869
                     /locus_tag="VG274_12445"
     CDS             1192..1869
                     /locus_tag="VG274_12445"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWE07515.1"
                     /translation="MKDGPSIAPIAALAGDPARANILGALLGGKALTAGELANEAGIT
                     AQTASSHLAKLEEGGLITGVRQGRHRYFKLAGHDVAEMLETMMGVAQRAGHTRTRTGP
                     KAPELRKARVCYDHLAGEIGVAMFDGLVKARLIAGGEMLRLTKRGEDFARDFGIDLDA
                     LSGERRPLCKSCLDWSQRRSHLAGGLGAAMLDRFYDLKWACRDRASRAVHFSPDGERA
                     LRRLLHI"
     gene            1939..>2298
                     /locus_tag="VG274_12450"
     CDS             1939..>2298
                     /locus_tag="VG274_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07516.1"
                     /translation="MSHKTFTLFLAPSVAALSFSIAAPVGAATFTTFDPPGAGETLPY
                     SINASGMITGFYITSNDKGHGFVRETDGTITVFDAISKAAYTRPAGFDADGNIAGSFT
                     DHHRKSRGFIRSTSGTVT"
BASE COUNT          461 a          738 c          710 g          389 t
ORIGIN      
        1 cgcgccgccc ccgcctcccg cgccgcgtca tcaccatgtt cacgttgcgc agtccgccac
       61 gcccgatgca ccgcctgcgc cgcccgcttc gcctgcaccc gaagcgatgt cggctccgcc
      121 tgcaccaccg cccccgccgg caatgcatgc cgtaccgccg gtaccatcgg tcccggctat
      181 cgctctcaat gaagacgatg aggacgtgaa tgtggacgtc gacaatgacg acgataccgt
      241 cagcgtgaag ggcgacaagg tcgtgaagat gcgcgtctgg cgcgacaaag agggccaccg
      301 tcatgtcata cggtccgagc gttttctcag ccctgcagac aaagcgcgca tccacgaaga
      361 agtggcgcgt gcacaagttg aggcgcgccg cgccgaggtc gaaatcgcgc gcgttcagcc
      421 ggaagtcgat aaggcgctcg ccgctgcgaa gatcaacgag aaggtcgcca aggcgatgcg
      481 cgaagccgag ccgcgcatcc gcgccgagat cgatcgcgcg atggcggagg ccaagccgga
      541 aatccgccgc gccatggccg aagcgcatat caatgagaag gtgatgaagg ctctgcgcga
      601 cgcacagccg aagatcgatg ccgccatcgc caaggcgcac gacaaggaat ggcacgcccg
      661 cgtcatgcaa gaggaacgcg acgatgcgtc cgacaaatac gacgacaatg acaatgatga
      721 cagccaggat cagaacgcac actaatcgtt ctgcggatcg ttcgtcatat ctgccggagg
      781 cgcggaagcg ctctccggca gttccgtttc aggccttcac aggctgcaga aatcggcgct
      841 cttcccgcac gatgaatttc tcgcgctgtg cgaattcgaa attggcgacg ctttttggat
      901 cgttgcgcag acaggcgcga tagttctcat acgccgctag gctctcgaac ccgatcatcg
      961 cgtgggcctc gtagttcgtg ccttccagcg gcaggaagta ccctatcagc gcgccgccgc
     1021 aggccgggat cagcgtcagc cacgcctttg cataagcttc aaacgcggcg cgcttgaacg
     1081 ggtcgatctc gtagcggatg tggcagacga cgttcatgct tccatctcca tcgaagtgtg
     1141 caggcaggat agggcttgcc tatgcgctaa tgcttcggcc aacatcgaag tatgaaagac
     1201 ggtcccagca tcgcccccat cgccgcgctc gcgggcgatc cggcgcgcgc caacattctc
     1261 ggcgccctgc tgggcggcaa agcgctgacg gccggcgaac tcgccaacga ggccggcatt
     1321 acggcacaga ccgcgagttc gcatctggcg aaactggaag agggcggcct gatcacgggc
     1381 gtacggcagg ggcgccaccg ctacttcaag ctcgccggcc acgacgtcgc cgagatgctg
     1441 gagacgatga tgggcgtcgc acagcgcgcg ggtcacacgc gaacacgcac cggcccgaag
     1501 gcgccggagc tgcgcaaggc acgcgtctgt tacgaccatc tcgcgggcga gatcggtgtc
     1561 gcgatgttcg atggtcttgt gaaagcgcgt ctgatcgcgg gcggcgagat gttgcgattg
     1621 acgaagcggg gggaggattt cgcgcgcgat ttcggcatcg atctcgatgc tctgtcaggc
     1681 gagcgcaggc cgctgtgcaa atcctgcctc gattggagcc agcgccgttc gcatctggcc
     1741 ggcgggctcg gtgccgcaat gctcgaccga ttctacgatc tcaaatgggc atgccgcgat
     1801 cgtgccagcc gcgctgtcca tttctcgccg gacggcgaac gcgctctgcg tcggcttctc
     1861 cacatttgaa cgtatcgtcc ggcaattacc acgaaaattg gcagcaatgg cccagaacct
     1921 tgggtgatag ggaggggaat gtcccacaaa actttcacgc tgtttctcgc accaagcgtc
     1981 gccgcgcttt cgttcagcat cgctgcaccc gtcggcgccg cgacattcac cacgttcgat
     2041 cccccgggcg ctggcgagac gcttccctac agcatcaacg cgtcgggcat gatcacgggc
     2101 ttctacatta ccagcaatga caaggggcat ggcttcgtgc gcgagaccga cggaacgatc
     2161 actgtcttcg atgcgatctc gaaagcggca tacacacggc ccgcgggctt cgacgcggac
     2221 ggaaacatcg ccgggtcctt cacggatcac cacaggaaat cgcgcggctt tatccgctcg
     2281 acgagtggga cggtcacg
//
LOCUS       DASZMC010000614         7504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4652863,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000614 DASZMC010000000
VERSION     DASZMC010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4652863"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VG274_12455"
     CDS             <1..770
                     /locus_tag="VG274_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07517.1"
                     /translation="TSILFTLTTGAAVGMSVADATHPAAIPSGTTVMALTASHVTLSQ
                     ATLGGPTGLANNDVINFTSAGGVTVTLSPGVYILNGQGGGSCTGSVGASKATACHSGD
                     FDVTNRATVTGTGVTIVLTTNTVYGYDVGNMFIDDNSALNITAPTSSVSGYPVAGVAI
                     WQNPLAPKPANPGPGYAEGAQGVNTVASGATTNITGLIYFPTQAVLFSGGAGSDACTQ
                     VLAWAVQFLRGANFSYPANCPATAGVQPIGGAPALTE"
     gene            856..1365
                     /locus_tag="VG274_12460"
     CDS             856..1365
                     /locus_tag="VG274_12460"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HWE07518.1"
                     /translation="MNTQSDGPKHGGTRRRRPLLIRIAQSNQGLAALEFALLAPVLMA
                     LLVGLLDFGLAFQRQLQVNAAAQAGASYAVINGFDVTGITNIVTAGSSGIQASPAPTQ
                     VCGCPGATSGITTSSPSTPPCTGKCPDGSTAGVYVSANALLSYSTLLPWPGITNPLSL
                     SSTEMVRTQ"
     gene            1362..1856
                     /locus_tag="VG274_12465"
     CDS             1362..1856
                     /locus_tag="VG274_12465"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HWE07519.1"
                     /translation="MRRAPIAAMPANFARAKLGRRPEPYSVRDWSMNRLVRLWRACEA
                     SAAIETAFILPVLLLGTLGTVEGARALWTQSSLQSAVVEAARCAAISPGSCTNVPTYA
                     ASQISWLGIPANDFSYSSTATCGVTGYAKGSQVAVSYTFQSVVNGLIPPLSHLTLTAS
                     ACHP"
     gene            2043..3185
                     /locus_tag="VG274_12470"
     CDS             2043..3185
                     /locus_tag="VG274_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07520.1"
                     /translation="MRSCATRRCSKIIAALVLCTAPAAAAADGFLETVHRHVMLTSTV
                     TDNGDLNPYAVVVAPVAAGPIHKGDVLIDNFNDLSNLQGTGTTIVDYNPATKATTLIA
                     RLPRQGTQCRGGVGLTTAMTMLKSGWIIVGSTPSIDGTTRTKGDGCLFVLDPDGRIAA
                     TWTGPDIGGPWGNIAAIDRGATATLFVSMAGKGVPGPDMRDPATGYPVTLHNATVVRF
                     DLSIAPGDPPVIEHQTVIADGFEQRADRDAFLIGPTGLALAGDGTLYVSDALGNRIVA
                     VPDAAMRGTSAGLGRTITRDGLLQRPLALALAPNGNLLVCNAKNGQVVEIDPATGKQL
                     SAQWIDADQAQSPPGNGDLFGLAMTPDGSGFYYVEDDVNALMEAKR"
     gene            3182..4459
                     /gene="efeB"
                     /locus_tag="VG274_12475"
     CDS             3182..4459
                     /gene="efeB"
                     /locus_tag="VG274_12475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HWE07521.1"
                     /translation="MSGEDARCASRRGFLQRASLLAAVAGSGFAPAQIAEARPEATSP
                     GKAIEPFFGARQGGILTPAQQHSYFAAFDLESEKKDDVVALLRNWTRASALVTQGKPA
                     DFSPQDLYAAGASTETLGLAPARLTLTFGFGAGLFVKDGRNRYGLAGARPDALIDMPR
                     FPGDQLVEARTGGDVSVQACADDPQVAFHAIRQLAQLACGTARLRWGQTGFLPHSPPN
                     ETPRNLMGFKDGTGNPAVQDEAAMTKFVFVGNEGPAWMRGGSYVVARRILIALEHWDR
                     TKVDFQEQTMGRHRATGAPLGKPGEFDALDFDAADTNGDPVTPVNSHVRLAAAASNDG
                     TRILRRGYSYNDGVSYTAERWPPWHQGMEYDAGLFFVGYQRDPREGFVKIFSKLAKFD
                     MLNQFSTHTGGGLFACPGGIAPGEFIGEKLFAA"
     gene            4729..5076
                     /locus_tag="VG274_12480"
     CDS             4729..5076
                     /locus_tag="VG274_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HWE07522.1"
                     /translation="MTHEFARKLRREQTEVERKLWYALRDRRFHGIKFRRQQPVGSYV
                     ADFICFEARLIVELDGSQHAFPENAVAERIRTAFLEREGFRVLRFWNLELKNNFDGVM
                     ETIRLAVLPLTRA"
     gene            complement(5270..6241)
                     /locus_tag="VG274_12485"
     CDS             complement(5270..6241)
                     /locus_tag="VG274_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWE07523.1"
                     /translation="MDVGFFNIFFDARFDATALAALFAFATIMTLGIPLLERNQLGAR
                     MKAVAQRREELRRKHHAALKNRGGLRTEPLTFIRQTVEGLNLSRIVEGEQVREKLARA
                     GYRGQGALVTFIFFRFVMPFIVFAAALIFLFAASPFKWSAFLKFNAALGAGLAGFYLP
                     DLFVTNLINKRQASIMRSFPDALDLMLICVEAGMSIETAFTRVSAEVGAQSPELAEEL
                     GLTTAELSYLPDRKQAYDNLAKRCAHSGVRAVAAAVNQAEKYGTPVGQALRVTAHENR
                     EQRMLEAEKKAAALSPKLTVPMLLFFLPCLFVVILGPAIENIMHTMK"
     gene            complement(6363..7349)
                     /locus_tag="VG274_12490"
     CDS             complement(6363..7349)
                     /locus_tag="VG274_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWE07524.1"
                     /translation="MSATILIALTALLAALGAGGTVFAFTTPAGASKKRLAAVARPTT
                     ASSALARASTAAQGQQKRKAMQNLLKDLDRQQAERKKRPTLQRRIARAGLDFSPRTFW
                     ISSAAAAVITAFACLLTRQPIVVVVLAGFATGLGLPRWAIHFLTKRRQKQFTNEFANA
                     IDVIVRSVKTGLPVNEALKIVATEMSEPVKSEFAKLSEGLKVGVTMEQGLKRMFDNMP
                     TPEVNFFAIVMTVQLKSGGNLSEALGNLAAVLRDRKRLQGKIKALSSEAKASAMIIGC
                     LPPGVTTMVYITTPDYIMPLFTTRWGNLLLAGCAIWMSIGTFVMKKMISFKT"
BASE COUNT         1345 a         2428 c         2423 g         1308 t
ORIGIN      
        1 taacttccat tctcttcact ctcacaaccg gcgctgcggt cggcatgagc gtcgccgatg
       61 cgacccaccc ggctgcgatt ccctccggca cgaccgtgat ggcgttgact gcgtcgcatg
      121 tgaccctgag ccaggccact cttggcggcc cgaccgggct cgcaaacaat gacgtcatca
      181 attttacctc ggcgggcgga gtcaccgtca ccttgagtcc tggggtttac atcctgaacg
      241 gccagggcgg cggcagttgc acgggcagcg tcggcgcctc gaaggccaca gcctgccaca
      301 gcggcgattt cgacgtcacc aatcgcgcga cggtcacggg gacgggcgtc acgatcgtgc
      361 tgaccaccaa cactgtctac ggatacgatg tcggcaacat gttcatcgac gacaattccg
      421 ccctcaacat cacggcgccg acctccagcg tatcgggcta cccggtggcg ggcgtcgcga
      481 tctggcagaa tcctctcgcc cccaagccgg ccaatccggg ccccggctat gcggagggcg
      541 cccagggcgt caatacggtc gcctcaggcg cgaccaccaa catcaccggc cttatctact
      601 tccccacaca ggccgtgctt ttctcgggcg gcgccggcag cgacgcctgt acgcaggttc
      661 tggcctgggc agtccagttc ctgcgcggag cgaacttctc ctatcccgcc aattgtcctg
      721 ccacggccgg cgtgcagccc atcggcggcg cgccagcgct caccgagtga accgcggcgg
      781 agcttggcca agattggccg ggagggcgcc cggcgaaagc gaaatcatcc cggacgaccg
      841 aagagagatc gagcaatgaa cacgcaatcg gacggaccca agcacggcgg cacgcgcagg
      901 cgccggccgc ttctcatacg catcgcgcag tcgaaccagg gtcttgcggc tttggaattc
      961 gctctgctcg cgccggtcct gatggcattg ctcgtcggct tgctggattt cggcctggca
     1021 tttcagcgtc agctgcaagt gaacgctgct gcacaagcag gcgcctccta cgccgtcatc
     1081 aacgggttcg acgtcacggg aatcacgaat atcgtgaccg ccggctccag cgggattcag
     1141 gcctcacccg cgccgacgca ggtttgcggg tgtcccggcg ccacatcagg gatcaccact
     1201 tcctctcctt caaccccgcc ctgcacgggc aagtgtccgg acggcagcac ggccggggtc
     1261 tacgtctcgg caaacgctct gctctcatac tcgacgctcc ttccctggcc aggcatcacc
     1321 aatcccctga gtcttagttc aacagagatg gtgcggaccc aatgaggcgc gcgcccatcg
     1381 ccgcgatgcc ggcgaatttc gcgcgggcga aattgggccg gcggccggag ccttattccg
     1441 tccgggactg gagcatgaac cgcctggtgc gcctctggcg cgcttgcgag gcctcggcag
     1501 cgatcgagac ggccttcatc ctgcccgtcc tgctgcttgg cacgctggga accgtcgaag
     1561 gcgcccgggc attgtggacc cagtcgtcac tgcagtccgc ggtcgtcgag gcggctcgct
     1621 gtgccgccat cagccccggg tcgtgcacga acgttccgac ctatgccgct tcccagatat
     1681 cctggctggg gattccggca aacgatttca gctattcgtc aacggcgact tgcggagtga
     1741 ccggctacgc caaaggatcg caagtggccg tgagttacac gttccagagc gtcgtcaatg
     1801 ggctgatccc gccactgtcg cacctcacgc tgacggcgtc ggcgtgccat ccctgacgcg
     1861 ttaaccgaaa gcctactgcc ggcgtcgtac aaagaccgcc gcgatccgcg aggctgcgat
     1921 tcgatggaat gggagcgtcc gcacgattgg tggcttccat cgacgatcgt aaccctggcc
     1981 ccgtttgggg catggattgt cggtttggcg gtgaggcagt ggttcggctg aattgttgtt
     2041 cgatgcgcag ttgcgccacg cgccggtgct cgaaaatcat cgccgcactt gtcctgtgca
     2101 cggcgccggc cgcggcggcc gccgacggat ttctggagac cgtccaccgc catgtcatgc
     2161 tcacctccac ggtgaccgac aatggcgatc tcaatcccta cgcggtcgtc gttgcgcctg
     2221 tcgcagcggg acccatccac aagggcgacg tgctgatcga caatttcaac gatctctcca
     2281 atctgcaggg cacgggcacc acgatcgtcg actacaatcc ggcgacgaag gcgacaacgc
     2341 tcatcgcccg cctgcccagg caaggaacac agtgccgggg cggcgtgggg ctgacaacgg
     2401 caatgaccat gctcaagagc ggctggatca tcgtcggcag cacgcccagc atcgacggca
     2461 cgacgcggac gaagggcgac ggctgcctct tcgtactcga tccggacggc cggatcgccg
     2521 cgacctggac agggccggat atcggcgggc cgtggggcaa tattgccgcg atcgaccggg
     2581 gcgcgaccgc cacgctgttc gtcagcatgg ctggcaaggg cgttcccggc cccgacatgc
     2641 gcgatcccgc gacgggctat cccgtgacgc tgcacaacgc cacggtcgtg cggttcgatc
     2701 tctccatcgc gcccggggat ccgccggtca tcgaacacca gaccgtgatt gccgacgggt
     2761 ttgaacagcg cgcggacagg gacgcgttcc tgatcggtcc cacgggactc gcgcttgcag
     2821 gcgacggcac gctgtatgtt tcggacgcgc tcggcaaccg gatcgtcgcc gtaccggatg
     2881 cagcgatgcg cggcacgagt gcggggctcg gacgcacgat caccagggac gggctgctgc
     2941 aacgcccgct cgcgctcgcg ctcgcgccga acggaaatct gctcgtctgc aacgcgaaga
     3001 acggacaggt cgtggagatc gatccggcga cgggcaagca gctctctgcg cagtggatcg
     3061 atgcagacca ggcgcagtcg ccgccaggca atggcgatct cttcggtctt gccatgactc
     3121 ccgacgggag cggcttctat tacgtcgagg acgatgtgaa cgcgctgatg gaagcgaaac
     3181 gatgagcggc gaagacgcgc gctgcgcttc gcggcgcggc tttctgcaac gcgccagcct
     3241 cctggctgcg gtcgccggca gcggatttgc gccggcgcag atcgccgaag cgcggccgga
     3301 ggcgacctcg cccggcaagg cgatcgagcc gttcttcggc gcccgacagg gcggcattct
     3361 gactccggcg caacagcaca gctacttcgc cgcgttcgat ctcgaatcgg aaaagaaaga
     3421 cgacgtcgtc gcgctgttgc gcaattggac gcgagcctcg gccctggtga cgcaaggcaa
     3481 accggcagac ttctctccac aggatcttta tgcggccggc gccagtaccg agacgctcgg
     3541 acttgcgccc gcgcggctca cgctcacatt cggtttcggc gcgggcctct tcgtcaagga
     3601 cggcaggaac cgttacggcc tcgccggcgc gcgtcccgat gcgctcatcg acatgccgcg
     3661 ttttccaggc gatcagctcg tcgaggcgcg caccggcggc gatgtttcgg tgcaggcctg
     3721 cgccgacgat ccgcaggtcg cctttcacgc cattcgccag ctcgcgcagc tcgcctgcgg
     3781 caccgcacgc ctgcgctggg gccagaccgg attcctgccg cattcgcctc ccaatgaaac
     3841 gccgcgcaac ctgatgggat tcaaggacgg caccggcaat cccgccgtcc aggatgaagc
     3901 ggcgatgacg aaattcgtct tcgtcggaaa tgagggaccg gcgtggatgc gcggcggcag
     3961 ctatgtcgtc gcgcgccgca tcctgatcgc gctcgagcat tgggaccgca ccaaggtcga
     4021 tttccaggaa cagacgatgg gccggcaccg cgcgacgggt gcgccgctcg gcaagccggg
     4081 cgagttcgac gcgctggatt tcgatgcggc cgacacgaac ggcgatccgg tcacgcccgt
     4141 caattcgcac gtaaggctgg ccgccgccgc aagcaatgac ggcacgcgga ttctgcggcg
     4201 gggctattcc tacaatgacg gcgtgagcta caccgcggaa cgctggccgc cctggcacca
     4261 gggcatggaa tacgacgcgg gcctgttttt cgtcggctat cagcgcgacc cgcgcgaggg
     4321 cttcgtcaag attttctcca aactggcgaa gttcgacatg ctgaaccagt tctcgaccca
     4381 taccggcggc ggactcttcg cctgccccgg cggcatcgcc cccggcgaat tcatcggcga
     4441 aaagctgttc gccgcgtaga ccgcccgcgc cgtgctcccc ctcacccgcg cctgaacaga
     4501 attcgccgcc cgtatctgca cgcctctccc gtccacctac gccaaggcta cggcggacag
     4561 agagagtcac cagacccgcc gaagctttag cgcaggcggg caaggggaga ggggaagcgg
     4621 agtcttgcat caggattggg aaaatcagcg cgcggagtgc cctctcccct tgcgggagag
     4681 gggccaagca ggaaacatcg caaccgcatt agcctgggtg agggggccgt gacgcacgaa
     4741 ttcgcccgca agcttcggcg cgaacagacg gaagtcgagc gcaagctgtg gtacgcgctg
     4801 cgtgaccggc gcttccatgg aatcaaattt cgccggcagc aacccgtcgg atcttacgtc
     4861 gccgatttca tctgcttcga ggcaagactc atcgtcgaac tcgatggaag ccagcatgca
     4921 tttccggaaa acgcggtggc ggaacggata cgaacggcgt tcctcgagag ggaagggttt
     4981 cgcgtgttgc gcttctggaa tctcgaattg aaaaacaact ttgacggagt gatggaaacg
     5041 atccgtctcg ccgtgctccc cctcacccgc gcctgaacag aattcgctgc ccgtatcggc
     5101 atccctctcc cacgagggga gaggggaatc ggagtcttgc ctcaggattg ggaaaatcag
     5161 cgcgcggagt gccctctccc cttgcgggag aggggtcaag aacgaggacc ttcaaccatt
     5221 caagcgcggg tgaggggtgc agccgctcag gcgcgggtga ggggcggcct tatttcatcg
     5281 tgtgcatgat gttttcgatg gcggggccga ggatgaccac gaacaggcag ggcaggaaga
     5341 agagcagcat cggcacggtg agcttgggcg acagggcggc ggctttcttc tccgcctcca
     5401 gcatgcgctg ctcgcggttt tcgtgcgcgg tcacgcgcag cgcctgtccc accggcgtgc
     5461 cgtatttctc cgcctggttg actgcggcgg ccacggcgcg gacgccggaa tgggcgcagc
     5521 gtttggcaag attgtcatag gcctgcttgc ggtcgggcag ataggagagt tcggcggtgg
     5581 tcagcccgag ctcctccgcc agttcgggcg attgcgcgcc cacctcggcg gaaacgcgag
     5641 tgaaggccgt ctcgatcgac atcccggctt cgacgcagat cagcatcagg tcgagcgcgt
     5701 cggggaagga gcgcatgatc gaagcctggc gcttgttgat caggttggtg acgaagaggt
     5761 cgggcagata aaagccggcg agtccggcgc cgagtgcggc gttgaacttc aggaaggccg
     5821 accatttgaa gggcgaggcg gcgaagagga agatcagcgc cgccgcgaag acgatgaacg
     5881 gcatcacgaa gcggaagaag ataaaggtga cgagcgcgcc ctggccgcga tagccggcgc
     5941 gggcaagttt ttcgcggacc tgctcgcctt cgacgatgcg cgagagattc agtccctcca
     6001 ccgtctggcg gatgaaggtc aggggttcgg tgcgcaatcc gccgcggttc ttgagcgcgg
     6061 cgtggtgctt gcggcgcaat tcctcgcgcc gctgcgccac cgccttcatg cgcgcgccca
     6121 actggttgcg ctcgagcagc ggaatgccca gcgtcatgat ggtggcgaag gcgaaaaggg
     6181 cggcgagcgc ggtcgcatcg aaccgcgcat cgaagaagat gttgaagaag ccgacgtcca
     6241 tgaacttcga tccttcctgc cgccgtggta ccagctatcg ttgatacctg actgggactt
     6301 catccttcga caggctcagg atgagggcct catggtgagc ctgtcgaacc atgagcgcag
     6361 ccctatgtct tgaacgagat catcttcttc atcacgaagg tcccgatgga catccagatg
     6421 gcgcagcccg cgagcagaag atttccccac cgcgtggtga acagcggcat gatgtaatcc
     6481 ggcgtcgtga tgtagaccat cgtcgtcacg ccgggcggca aacaaccgat gatcatggct
     6541 gaggccttgg cttccgacga aagcgccttg atcttgccct gcaggcgctt gcggtcgcgc
     6601 agcaccgcgg caagattgcc gagcgcctcg gacaggttgc cgcccgattt cagctgcacc
     6661 gtcatcacga tggcgaagaa attcacttcg ggcgtcggca tgttgtcaaa catgcgcttc
     6721 agcccctgct ccatggtgac gccgaccttc aggccttcgg agagctttgc gaattcgctt
     6781 ttgaccggct cgctcatttc ggtcgccacg atcttgagcg cttcgttgac gggcaggccg
     6841 gttttcacgc tgcgcacgat tacgtcgatg gcgttggcga actcgttggt gaactgcttc
     6901 tgccgccgtt tggtgaggaa atgaatcgcc cagcgcggca ggccgaggcc ggtcgcgaag
     6961 cccgccagga cgacaaccac gatgggctgt ctggtcagca ggcaggcaaa ggcggtgatc
     7021 accgcagcgg cggccgagga aatccagaac gtccgcggcg aaaaatccag gcccgcccgg
     7081 gcgatgcggc gctgcagggt cggtcgcttt tttcgttcgg cctgctggcg gtccagatcc
     7141 ttgagcaggt tctgcatcgc cttgcgcttc tgctggccct gggcggcggt cgacgcacgc
     7201 gcaagcgccg acgaggcggt tgtcggccgc gccacggcgg ccaggcgttt cttcgacgct
     7261 cccgcgggcg ttgtgaaggc gaacaccgtt ccgcccgcgc cgagagcggc gagaagcgcg
     7321 gtcaatgcta tgaggatcgt ggcgctcatt ttacttcacc gtcactgctg aagggcgtcg
     7381 agcgcctctg cgagttcacg ctccaggttg aaatagcgcg cgcgctccca gaaggtaggg
     7441 cgcacgatgc ccgtgcccgc atgacggccc ttgatgcggc cattgtcgtc ttccccggcc
     7501 atct
//
LOCUS       DASZMC010000615         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3011100,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000615 DASZMC010000000
VERSION     DASZMC010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3011100"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VG274_12495"
     CDS             <1..547
                     /locus_tag="VG274_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWE07525.1"
                     /translation="AASLFKIAQDIRLLGSGPRSGLGELQLPENEPGSSIMPGKVNPT
                     QCEALTMVCTQVMGNHVTITIADSQGNFELNVFKPVLIYNLLQSIRLLGDAALSFTEH
                     CVNGIEANRTRIAELLERSLMLVTALAPTIGYDKAATVAKTAHKNGTTLRDEAVRLGF
                     VTAQEFDRLVRAEEMTRPKTA"
     gene            570..803
                     /locus_tag="VG274_12500"
     CDS             570..803
                     /locus_tag="VG274_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWE07526.1"
                     /translation="MAQLLKKRSFSIAGHRTSVAVEPEFWTVLEHEAHRTGLSLSAFV
                     AQIDSARGTRPLASALRLAALAADSRIAASSSR"
     gene            847..1029
                     /locus_tag="VG274_12505"
     CDS             847..1029
                     /locus_tag="VG274_12505"
                     /inference="COORDINATES: protein motif:HMM:NF025143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HWE07527.1"
                     /translation="MGELINLRRVKKSKRRTETEKSAAANRMVHGTPKELREAAEAEK
                     RRAERTLGAHKIENED"
     gene            complement(1039..1875)
                     /locus_tag="VG274_12510"
     CDS             complement(1039..1875)
                     /locus_tag="VG274_12510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE07528.1"
                     /translation="MTQKVVVVTGASSGIGEVAADRLAAKGARIVFVARDRSRGEETL
                     KHLQAIAPAAEHSVHYADLTRLAEMKRVAAEIAESEPEIDILVNNAGALFNARKVTED
                     GLEMTFALNHMAYFVLTGVLRPRLAAGARIVSTASDAHKGAQLDFSDLQSERNYSGFG
                     AYGRSKLCNILFTRELARRLKDTGVTANCLHPGFVATRFGDQSGGVMSLAVRAAKNFA
                     ITPEQGAQTMVYLASSPEVAEITGGYFYKCRQATPTREAQNDDDARRLWDASTRIAGI
                     AA"
     gene            complement(1885..2307)
                     /gene="ndk"
                     /locus_tag="VG274_12515"
     CDS             complement(1885..2307)
                     /gene="ndk"
                     /locus_tag="VG274_12515"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWE07529.1"
                     /translation="MATERTLSIIKPDATRRNLTGKIAARLEDGGLRIVAQKRMQLSL
                     GAAEAFYNVHKERPFFRSLVEFMSSGPVVVQVLEGENAIARNREIMGATDPAKAAPGT
                     IRKDFAESIEANCVHGSDSAETAMHEIRFFFSELDIVD"
     gene            2411..>3519
                     /locus_tag="VG274_12520"
     CDS             2411..>3519
                     /locus_tag="VG274_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWE07530.1"
                     /translation="MLSIENLTYRIAGREILSGANAHLPAGRRIGLVGRNGAGKSTLL
                     RLVLGDAVPDGGDISRPAAWRIGSVAQEAPGGDASLIDTVLATDDERQRLLAESETER
                     DGHRLGEIHARLDAIDAYAAPSRAASILAGLGFSASEQLRPCREFSGGWRMRVALASI
                     LFAAPDLLLLDEPTNYLDLEGVLWLEDYLRRYRGTVIIVSHDRDLLDALAEFILHLEH
                     GRLTLYSGNYETFAETRGARRALDAAFARKQETARRHMQEFVDRFRYKQSKARQAQSR
                     LKMIARLKPIDIPLDEHVAPIRLPEPSPSSPPLLSLDRIAVGYDPGRAVLEEVSFRLD
                     PDDRIALLGRNGNGKSTLAKLIAGKLAHFSGEMVRA"
BASE COUNT          650 a         1126 c         1105 g          638 t
ORIGIN      
        1 ggccgcctcg ctgttcaaga tcgcgcagga catccggttg ctgggttccg gtccgcgatc
       61 gggcctcggc gagctgcagt tgccggagaa cgagcccggc tcttccatca tgcccggcaa
      121 ggtcaacccg acccagtgcg aagcgctcac catggtttgc acgcaggtga tgggcaacca
      181 cgtcaccatc acgattgccg acagccaggg caatttcgag ctgaacgtgt tcaagccggt
      241 gctgatctac aatcttctgc aatcgatccg gctgttgggc gatgcggcct tgagctttac
      301 cgaacattgc gtcaatggca ttgaggccaa ccgaacgcgc atcgcggagc ttcttgagcg
      361 ctcgctcatg ctcgtaaccg cgctggcgcc gacgatcggc tacgacaagg ccgcgacggt
      421 cgccaagacg gctcacaaga acggcacaac cctgcgcgac gaagcggtga ggctcggatt
      481 cgtcacggcg caggaattcg accgtctggt ccgcgccgaa gagatgaccc gccccaaaac
      541 ggcgtaggcc gttggcggtt gaccctgccg tggcgcagct cctgaagaag cggtccttca
      601 gcattgcagg ccaccgcacc agtgtcgccg tcgagcccga attctggacc gtgctcgaac
      661 acgaggcgca tcggacggga ctgagcctct cggccttcgt tgcgcagatc gactccgcgc
      721 gcgggacgcg accactcgcc agcgcgctca ggctcgccgc actcgccgcc gattcgcgga
      781 ttgctgcttc ttcttcgagg tagttgcggg taatctcgcg gcggctccac ggtaaaagtc
      841 cccggcatgg gcgaactcat caatctgcgt cgcgtcaaga aatcgaagcg gcgcaccgag
      901 accgaaaaaa gcgctgcggc caatcgcatg gttcacggca cgccgaagga gctgcgtgag
      961 gcggcagagg ccgaaaagcg gcgtgccgag cgaacactcg gcgcgcacaa gatcgagaac
     1021 gaagattaac gggcagcgct atgctgcgat gccggcaatc cgcgtcgatg cgtcccacag
     1081 acgccgcgcg tcgtcgtcgt tctgcgcttc ccgcgtcggc gttgcctggc ggcatttgta
     1141 gaaatatccg ccggtgatct ccgccacttc gggcgaggac gcaagataga ccatggtctg
     1201 cgccccctgc tccggcgtga tcgcgaaatt cttggccgca cgcacggcga gagacatcac
     1261 gccgccggac tgatcgccga agcgcgtggc gacaaatccc ggatgcaggc aattggccgt
     1321 gacgcccgtg tccttcaaac gccgcgcaag ctcgcgcgtg aaaaggatgt tgcagagctt
     1381 gctgcgtcca tatgcgccga agccgctgta gttcctctcc gactgcaaat ccgaaaaatc
     1441 gagctgcgcg cctttgtgcg catccgacgc cgtcgacacg atgcgcgcgc cggctgcgag
     1501 acgcggacgc aggacgcccg tgagaacaaa ataggccatg tgattgagcg caaaggtcat
     1561 ctccaggccg tcttcggtga ctttacgtgc gttgaacagc gcgccggcat tgttgacgag
     1621 aatgtcgatc tcgggttcgg attccgcgat ttctgcagcg acgcgcttca tctcggcgag
     1681 gcgcgtcagg tcggcgtagt ggacactatg ttccgcggcg ggtgcgatcg cctgaagatg
     1741 cttcagcgtc tcttcgccac gggacctgtc gcgagcgaca aagacgatgc gcgcgccctt
     1801 ggccgcaagg cggtcggcgg cgacctcgcc gatccctgag cttgcgccgg tcacgacgac
     1861 gactttctgg gtcattgcgg cgactcagtc gacaatatcg agttcgctga agaagaagcg
     1921 aatctcgtgc attgcagttt cagccgagtc cgagccgtga acgcaattgg cttcgatcga
     1981 ttcggcgaaa tccttgcgga ttgtccctgg tgccgccttg gcgggatcgg tcgcgcccat
     2041 tatttcccga ttgcgggcga tggcattctc gccttcgagc acctgcacga cgaccggtcc
     2101 cgaactcatg aattcgacga ggctgcggaa gaacggacgc tccttgtgca cattgtaaaa
     2161 ggcttcggcc gcgccaaggg acaattgcat gcgcttctgc gcgacaatgc ggagtccgcc
     2221 gtcctccagc cgcgccgcga tctttccggt aagattgcgc ctggtggcat ccggcttgat
     2281 gatcgacagc gtacgctcgg tcgccattct cgcactccat tccgtatttc aacgcgcgcg
     2341 ccttatagaa gccgtcctgt tgcgccgcaa gacatggtag gagctgcccg cctccgcgag
     2401 cgaaacgacc atgttgtcga ttgaaaacct cacctaccgc atcgccggcc gcgagatcct
     2461 gagcggcgcg aatgcgcatc tgccggccgg ccggcgcatc gggcttgtgg gacgcaacgg
     2521 cgctggcaaa tcgactctgc tcaggctggt gctcggcgac gccgtgccgg acggcggcga
     2581 catttcgcgg ccggcggcat ggcggattgg cagcgtcgca caggaggcgc cagggggcga
     2641 tgcaagcctc atcgatacgg tcctggcgac ggatgatgaa cggcagcgcc tgcttgcgga
     2701 aagcgagacg gaacgcgacg gccaccggtt gggtgaaatt catgcgcggc tcgacgccat
     2761 cgatgcctat gccgcgccgt cgcgcgccgc ttcgattctg gcggggctcg gattctccgc
     2821 gtccgaacag cttcgtccgt gcagggaatt ctcgggcggt tggcgcatgc gcgtcgccct
     2881 cgcaagcatt cttttcgcgg cgcccgatct gctgcttctc gacgaaccga ccaactatct
     2941 cgatcttgaa ggcgtgctgt ggctcgagga ttaccttcgc cgttatcgcg gaacggtgat
     3001 catcgtcagc cacgaccgcg atctgctcga cgcgctcgcg gaattcatcc tgcacctcga
     3061 gcacggccgc ctcacgctct acagcggcaa ctacgaaaca ttcgccgaaa cccgcggggc
     3121 gcggcgcgcg ctggatgcgg cattcgcgag aaagcaggaa accgcgcgcc gccacatgca
     3181 ggagtttgtc gaccgcttcc gctacaagca gagcaaggcg cgccaggcgc aaagccggct
     3241 caagatgatt gcgcggctga agccgatcga catccctctc gacgagcacg tcgcgccgat
     3301 ccgcctgccc gaaccgtcgc cttcgagtcc gccgctgctg tcgctcgaca ggattgccgt
     3361 gggttatgac ccgggccggg cggtgctcga agaggtctca ttcagactcg atcccgatga
     3421 ccggattgcg ctgctgggcc ggaacggtaa cggcaaatcg actctcgcca agcttattgc
     3481 gggcaagctt gcgcatttct ccggagaaat ggtgcgcgc
//
LOCUS       DASZMC010000616         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3811385,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000616 DASZMC010000000
VERSION     DASZMC010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3811385"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VG274_12525"
     CDS             <1..501
                     /locus_tag="VG274_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07531.1"
                     /translation="VAGVSAAATGVTAAGLAAEWTARLKTLTAHEGETLLKMARQIFP
                     HDRLDDSYYMKVVEDLDIEASRKPVTGRMLRAGVANLDLSAKGKFAARPAAEQVSALE
                     KIQHTPFFEKVRSTEMVALYNNPDVWKVFGYQGASYPFGGYLHHGFNDLNWLPDPPEA
                     ASPKSG"
     gene            517..>1690
                     /locus_tag="VG274_12530"
     CDS             517..>1690
                     /locus_tag="VG274_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWE07532.1"
                     /translation="MARFDSNDDSVVVVIGSGAGGGTLANELCRKGLKVVMLEAGQRQ
                     SSQTFRNDEWFAFKQLAWLDKRTTSGTWRVAKDFPNLPAWSCKTVGGTTVHWAGASLR
                     FQEQEFKARTTYSNVSGANLLDWPISLADLTPYYARAEDKMGVTRTNDIPGLPGNNNF
                     KVMYNGATRVGYKTVHTGRMAINSRPSDRRASCWQLGFCFQGCKSGAKWSTLYTEIPK
                     AEATGKLDLRPESHALQIQHDANGKATGVLYADRNGKHVVQKARIVCIACNAIESARL
                     LLNSSSSTFPHGLANSSGQVGRNYMRHTTGSVYAIFDKPVHMYRGTVMAGIITDEAEL
                     NTKRGFAGGFEMETISLGLPFAAAFLDPGAWGRDYSSVLEQYPNMAGMWIVGEDMPQ"
BASE COUNT          360 a          507 c          526 g          297 t
ORIGIN      
        1 gtcgccggcg tgtccgcggc tgccaccggt gtgacggccg ccggcctggc cgccgagtgg
       61 accgcgagat tgaagaccct caccgcgcat gagggcgaaa cgctactgaa gatggcgcgg
      121 cagatatttc cgcacgaccg cctcgacgat agttactaca tgaaggtggt cgaggacctt
      181 gacatcgagg cgtcgaggaa acccgtaacc ggcagaatgc tgcgcgctgg agttgctaat
      241 ctcgatctct cggcgaaggg aaaattcgcc gcccgacccg ccgccgaaca agtcagcgcg
      301 ctcgagaaaa tccagcacac cccatttttc gagaaagtcc gcagtaccga gatggtcgcg
      361 ctctacaaca acccggacgt ttggaaggtc ttcggctacc agggcgcgtc gtaccctttc
      421 ggcggttacc tgcatcatgg cttcaacgat ctgaactggc tgcccgatcc acccgaagcg
      481 gcaagcccta aatcaggctg agcggagggt cgcgcagtgg ctcgattcga ttcaaacgac
      541 gactctgtcg tggtggtcat tggttcggga gcgggcggag gcacgctcgc gaacgaactg
      601 tgccgcaagg gcttaaaggt tgtgatgctg gaggcggggc agcggcagtc ctcgcagacc
      661 tttcgtaacg acgagtggtt cgcgttcaaa caactggcgt ggctcgacaa acgcaccacc
      721 tccggcacct ggcgcgtggc gaaggatttc cccaatctgc cggcctggag ctgcaagacg
      781 gtcggcggaa cgacggtgca ttgggcgggc gcttcgctgc gctttcagga gcaagagttc
      841 aaagccagaa ctacctattc aaatgtcagc ggtgcgaacc ttctcgactg gcccataagc
      901 ctcgcggatc tgacgccata ctacgctaga gccgaggaca agatgggcgt gacgcggacc
      961 aatgatatac ccggactgcc cgggaataat aacttcaaag taatgtacaa cggcgcgaca
     1021 cgcgtcggct ataagaccgt gcataccggg cggatggcga ttaacagccg gcccagcgac
     1081 cgccgtgcct cctgctggca gctcggcttt tgtttccagg gctgcaagtc cggggcgaag
     1141 tggtcgacgc tgtatacgga aattccgaag gccgaggcga ctggcaagct cgatctgcgg
     1201 cccgagtccc acgcgctgca aattcagcac gacgccaacg gcaaagcgac cggcgtgctg
     1261 tatgccgata ggaacggcaa acacgtggtg cagaaagcgc gcatcgtctg catcgcctgc
     1321 aatgcgatcg aaagcgcgcg gctcctgcta aactcgtcct cgtcgacctt tccacacggg
     1381 ctggcgaact cctccggcca ggttggcaga aactacatgc ggcacacgac cggctccgtg
     1441 tacgcgattt tcgacaagcc cgtgcacatg taccgcggca ccgtgatggc cggcatcatt
     1501 accgatgaag ccgagttgaa taccaagcgc gggtttgccg gcggcttcga gatggagacc
     1561 atctcgctcg gattgccatt cgcggccgcg ttcctcgatc cgggagcgtg ggggcgcgac
     1621 tactcaagcg tactcgagca gtatccaaac atggccggga tgtggatcgt cggcgaagac
     1681 atgccgcagg
//
LOCUS       DASZMC010000617         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2732226,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000617 DASZMC010000000
VERSION     DASZMC010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732226"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VG274_12535"
     CDS             <1..1028
                     /locus_tag="VG274_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07533.1"
                     /translation="LPEAIFAFGFAAALAVLPGDWRHRGARLAAGALGGLIGFTIFAA
                     FWMAAMARLTGNPVFPYFNEIFHSPLALAAPYRDLRFIPRSLAHQVLFPLLFSLDWRV
                     ADDLPFRDIRVGLAYVLFIATIPLWLVARRRANKLVAQDAAAALFAFAAVSYIAWLRM
                     FAIYRYILVLEMLAPILIAASVGLWPLSVRTRLVTTALLLLWAQASMKALVPGRAPAT
                     DPYVQAAFPPIPHPDRTMILMTGEAPLGYLVPSLPAQIPVLRIDGWLIQPQDGSRLTD
                     ETRKRVAGFRGDLFVIADAGELGRSRDALAVYGLAIGEAKCRNIVTNLGGPYRFCPLA
                     PVQNGVR"
     gene            1025..1969
                     /locus_tag="VG274_12540"
     CDS             1025..1969
                     /locus_tag="VG274_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWE07534.1"
                     /translation="MTEPAARIAVLVPCYNEEAAIGAVVRDFRAALPTAEIYVYDNAS
                     RDQTSQHARDAGAIVRHETRRGKGNVVRRMFADVEADIYVLVDGDDTYDASAAPMLVR
                     TLSEGGYDIVSGRRVAVGEHAYRTGHVLGNRLLTGLTALMFHVKLADLLTGYRIMSRR
                     FVKSFPFTAEGFGIETELTVHAARLLMPMIEIDTSYKERPAGSVSKLNTWGDGFRILF
                     TIAALVREERPLVFFAGVFALLATASLLVGAPVIVDYFRTGMVPRLPSAVLATALMLL
                     GFLALASGLILDTVTRGRWELKRTAYLRIPGPGDLKLP"
     gene            1966..2421
                     /locus_tag="VG274_12545"
     CDS             1966..2421
                     /locus_tag="VG274_12545"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWE07535.1"
                     /translation="MNFLAAGVKRIQEIRFVRFGLVGAGGFCVDTSVLWFMLHGIGLD
                     KYSGRGVSFMAAVTFTWWGNRILTFRDRAAKTGLLREWMTFVSANAIGGVVNLGLYTI
                     LVSFAPAPANNPFLAVFAGVLAGLSFNFLLSSRIVFRAPAKKRASPEPR"
     gene            complement(2404..2961)
                     /locus_tag="VG274_12550"
     CDS             complement(2404..2961)
                     /locus_tag="VG274_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWE07536.1"
                     /translation="MPLIPYADTRAAPQEVREAFARMPRKLNVMKMLANAPASFVPCM
                     RLGGAILGRQKLKPELRELVILLVSHLEGGAYEWVQHEPIAEAAGCRKEKIAAIEAGR
                     FDDACFDDREQTLLRLAHEVIEKVRADEDTVKSATRYFSSQEIVEIILTCGFYMMLAR
                     LTETTRTDMDTPSGTAVVEELARLR"
     gene            complement(3031..>3370)
                     /locus_tag="VG274_12555"
     CDS             complement(3031..>3370)
                     /locus_tag="VG274_12555"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWE07537.1"
                     /translation="AFDGGVLLVPHLDRLAGTALRVRLHADDIMLAREAPQAVSANNV
                     LPVEIVSVLAQHGAHADIGLRCGNVKLVARITCSSLERLGFAGGQHVFAIVKSVTVDP
                     QLGASRDHAP"
BASE COUNT          536 a         1113 c         1039 g          682 t
ORIGIN      
        1 aacttccgga agcaattttt gcgttcggct tcgcggcggc gcttgccgtc ctgcccggag
       61 attggcgcca tcgtggcgcg cggctcgcgg ccggtgcgct cggcggcctc atcgggttca
      121 cgatctttgc cgcattctgg atggccgcca tggcgcggct gaccggcaat ccggtctttc
      181 cctatttcaa cgagatattc cactcgccgc ttgcgctggc cgcgccctat cgcgatctcc
      241 gcttcatccc gcgcagcctc gcgcaccagg ttctttttcc gcttctcttt tcgctcgact
      301 ggcgggtggc cgacgatctt ccgtttcgcg acattcgcgt cggcctcgcc tatgttcttt
      361 tcatcgcgac catcccgttg tggctggttg cgcggcggcg cgcgaacaag ctcgtggcgc
      421 aggatgcggc cgccgcgctc ttcgcattcg ccgccgtctc ctacatcgcc tggctgcgga
      481 tgtttgcgat ctatcgctac atactggtgc tcgagatgct ggctccaatt ttgatcgctg
      541 catcggtggg tctctggccg ctttcggtgc gcacaaggct cgtcaccacg gcgttgctgc
      601 ttctctgggc gcaggcgtcg atgaaggcgc tcgtgccggg ccgcgcgccc gccaccgatc
      661 cttacgtgca ggccgccttc ccgcccattc cccatcccga ccgcaccatg atcctgatga
      721 cgggggaagc gccgctcggc tacctcgtcc cttcattgcc ggcgcaaatt cccgtcttgc
      781 gcatcgacgg ctggctgata cagccccagg acggctcgcg cctcaccgac gagacaagaa
      841 agcgggtcgc gggttttcgc ggcgatctgt tcgtgatcgc cgacgcgggc gaactggggc
      901 gcagccgcga tgcgctggcc gtctatggtc ttgccatcgg cgaagccaaa tgccgaaata
      961 tcgtcaccaa tctcggcggg ccctatcgat tctgcccgct cgcacccgtg cagaacggag
     1021 tccgatgacc gaaccggccg cgcgcatcgc cgtactcgtg ccctgctaca acgaggaagc
     1081 agcgatcggc gcggtggtgc gggatttccg cgctgcgctg cccaccgccg aaatctatgt
     1141 ctacgacaac gcttcgcgcg atcagacctc gcagcatgcg cgcgacgccg gcgccatcgt
     1201 gcgccacgaa acgcgccggg gcaaaggcaa tgtggttcgg cgcatgttcg ccgacgtcga
     1261 agccgacatc tatgtcctgg tcgacggcga cgatacctat gacgcctctg cagctcccat
     1321 gctggtgcgt acgctgagcg aaggcggcta cgacatcgtc tcgggacgcc gagtcgcggt
     1381 gggcgaacat gcctaccgca cggggcatgt gctcgggaac aggctgctca ccggcctcac
     1441 agccctcatg ttccacgtca agctcgccga tcttttgacc ggctaccgca tcatgtcgcg
     1501 gcgcttcgtc aaatccttcc ccttcaccgc cgagggcttc ggcatcgaaa ccgaacttac
     1561 ggtacacgcg gcgcgcctgc tgatgccgat gatcgaaatc gacaccagct acaaggaacg
     1621 gccggcaggg tcggtgagca agctcaatac ctggggcgat ggtttccgca tcctgttcac
     1681 gattgccgcg ctggtgcggg aagaacggcc gctggtgttt ttcgcgggcg tgtttgcgct
     1741 tttggcgacc gcttcgctgc tggtcggcgc gccggtgatc gtcgattact tccgcaccgg
     1801 catggtgccg cggcttccct ccgccgtgct cgcgaccgcg ctcatgctgc tcggctttct
     1861 cgcgctggcg agcggcctga tcctggatac ggtcacgcgc gggcgatggg agctcaaacg
     1921 cacggcatat ttgcgcattc ccgggcccgg agatctcaaa ctgccatgaa ctttctcgcg
     1981 gctggggtaa agcggattca ggaaattcgt ttcgtgcgtt tcggtctggt gggcgcgggc
     2041 ggcttttgcg tcgacaccag tgttctctgg ttcatgctgc acggcatcgg cctcgacaaa
     2101 tacagtggac gcggcgtctc cttcatggcg gccgtgacgt tcacgtggtg gggaaatcgc
     2161 attctgacct ttcgcgaccg ggccgcaaaa acggggttgt tgcgcgagtg gatgacattc
     2221 gtgtcggcca acgcgatcgg cggagtggtg aatctgggcc tttacacaat tcttgtgagc
     2281 tttgcgccgg cccccgccaa caatcccttc ctcgctgtct tcgcaggcgt tctcgcggga
     2341 ttgagcttca acttcctgct ttcgagccgc atcgtcttcc gggcgccggc gaagaagcgc
     2401 gcttcacctg agccgcgcta gctcctccac caccgctgtt ccggacggcg tgtccatgtc
     2461 ggtgcgcgtc gtctcggtca ggcgcgccag catcatgtaa aagccgcacg tcagaatgat
     2521 ctccacgatc tcctgcgacg agaaataccg ggtcgccgat ttgaccgtgt cctcatcggc
     2581 gcgaaccttt tcgatcacct cgtgcgccag tcgaagcaag gtctgctcgc gatcgtcgaa
     2641 gcacgcatcg tcgaagcggc cggcttcgat cgccgcaatc ttctccttgc ggcagcctgc
     2701 cgcctcggcg atcggttcgt gctgcaccca ctcataggcg ccgccttcaa gatgagacac
     2761 gagcagtatg accagctcgc gcaattcggg cttgagcttt tgcctgccga gaattgcccc
     2821 gccgagccgc atgcagggca cgaaggaggc gggcgcattc gccagcatct tcatgacgtt
     2881 gagcttgcgg ggcatccttg cgaacgcctc tcgcacctcc tgtggcgcgg cgcgggtatc
     2941 ggcatacgga atcagtggca tcggaacgct ccgcgctgtg cacttcgcgc acgagcatga
     3001 acgaggaaat gcccggtatg caacgggtgt tcagggcgca tggtcccggg atgcgccgag
     3061 ctgcggatcg acggtgacgg acttgacgat cgcgaaaacg tgctggccgc ctgcgaaacc
     3121 aagacgctca agcgaggagc aggtgatgcg ggccacgagc ttcacattgc cgcagcgcag
     3181 gccaatgtcg gcgtgcgctc cgtgttgcgc cagcaccgac acaatctcga ccggcaatac
     3241 attgttggcg ctgacggcct gcggcgcctc gcgcgccagc atgatgtcgt cggcgtgcag
     3301 ccgcacgcgc aaggcggtcc cggccaaacg gtcgagatgc ggcacgagca aaacaccgcc
     3361 atcgaatgcg
//
LOCUS       DASZMC010000618         5964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1895885,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000618 DASZMC010000000
VERSION     DASZMC010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895885"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2223)
                     /locus_tag="VG274_12560"
     CDS             complement(<1..2223)
                     /locus_tag="VG274_12560"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07538.1"
                     /translation="MRNKLYVSLAVLALSAGAFGLYAQTYTPKLYQAMRWRQIGPFRA
                     GRVTAVAGIPGNAAIYYMGTPGGGLWKTIDGGVIWTPIFDQVRSTSSIGAVSVALSDP
                     NIIYVGTGDVSMVGGSVNFGDGVYKSVDAGKTWQHIGLEETEHIGNLFIDPHDPNTVV
                     VAALGRTYSKNKQRGIFKTTDGGKTWRQTLAKDDTTGAVDLAFAPSNPKVGYAAIMEH
                     YTRPGARAAIESTGTAGVFKTTDGGDTWTQVTDGLPTGRLGRIGVATDATGQTVFAIV
                     AGGAGGAGGGAGGGGLYKSTDAGAHWAKSTPDTRITGSGYFSRVFLDPRNPKVVYVAQ
                     TSLYRSDDGGQTFSSYKGAPGGDDNHALWIDPLDSNRMMMASDQGGAISMDGGKSWSS
                     WYNQPTGQIYHLSTDNRYPYWVYGTQQDSGSVGTLSRGDYGAITFMDWDPVGGYEFGY
                     IVPDPLNNNLVYAGGPSRGLVRIDRTNRQVSTVSPSIGRGAANEYRMATNPPLAFSPQ
                     DGHVLYLGAQMLLETRDGGVHWKPISPDLTVRKEAPAVTESAATTPASPGAAQLVRPR
                     AQEQQETINPNNRAAINAFCPSPVAAGEIWVGSNNGVIQLTKDNGAHWQDVSPAGLAA
                     NTQISIIEASHFDANTAYAAIDRHEENDFKAHFYRTHDAGKTWQEISSGFADGDFARV
                     VREDPQRKGLLYAGTESAAYVSFDEGDHWSSLQLNMPVTSIRDLVIHGDDLVAATYGR
                     A"
     gene            complement(2253..3761)
                     /gene="cobA"
                     /locus_tag="VG274_12565"
     CDS             complement(2253..3761)
                     /gene="cobA"
                     /locus_tag="VG274_12565"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWE07539.1"
                     /translation="MSNPRSPKVYLVGAGPGDPGLITVKGRRVLEQADSVLYDHLASD
                     SLLDLAPPAAERLYVGKKKSAHAYSQEEICAMLIDRARRGFTVVRLKGGDPFIFGRGG
                     EEAEALADAGIPFEVVPGVTTPLGIAAYTGVPLTHREHTSAVTFVTGHAVDQIDWDRV
                     GMSETLVVFMGLTTFPAIAHELIVRGRAADTPAMAVRWATRADQETIVGTLESLPRLI
                     ESSHIKPPATIVVGEVVRLRPKLDWFERLPLFGCRIVVTRARAQADALGSKLAAFGAD
                     IVELPAIEIQPPADPAPLGAAIAALDSYDWLIFTSVNGVKHFIDRLDRSAIDLRKLRA
                     RICAIGPATRAAVEALHLKVDLMGKEYVAESLIEAFAAHDLTGRRVLLPRAAVARDLV
                     PAELTRRGAQVDIVEAYRTVAPAGLAERAREVFSAARKPGIITFTSSSTVQNLVAAAG
                     AESLRGIAAASIGPITTRTARDLGIEVPIEAREFTVDGLVDAILAAQPQRPL"
     gene            complement(3754..4662)
                     /gene="hemC"
                     /locus_tag="VG274_12570"
     CDS             complement(3754..4662)
                     /gene="hemC"
                     /locus_tag="VG274_12570"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWE07540.1"
                     /translation="MKLTIASRGSQLALWQARWIEARLIELGHECQIEIIKTTGDKFA
                     EASLAQLGAATGGKGLFTKEIEEALLDGRAHLAVHSLKDLPTTLPDGLALAAIPVRED
                     PRDAVVGKKLAELNPGDVIGTSSLRRAAQLLRLHPNLRIESIRGNLDTRLRKLDEGQY
                     SSIVLASAGLRRLGWENRIAEVLPPDVMCPAVGQGALAVETCTTGPGRDACAALDHAA
                     TRAAVTAERAFLRSLGGGCQVPIGAHAEVDGETLRIRGLVIAADGSQVVTGDRSGPSH
                     SAEELGASLGADVLSRGAQALLEVHV"
     gene            complement(4659..>5964)
                     /gene="hemA"
                     /locus_tag="VG274_12575"
     CDS             complement(4659..>5964)
                     /gene="hemA"
                     /locus_tag="VG274_12575"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWE07541.1"
                     /translation="GGALAFGSFAGPMKLAIFGVNHKSAPLAVRESLAFGEDVIPSAL
                     GDLKSQSGVCEAMILSTCNRVEITIASEDSTDPASLVDGFLAAHKGLDARKLGGQLYR
                     HEGRAAIHHLFRVASSLDSMVVGEPQILGQLKTAYGAAKECGAVTGWLDSLVSRAFSV
                     AKRVRSETGIGQMAVSISYAAVELARKIFGSLKGKNVMIVGAGKMSELAARHLRRSGA
                     TGIFVTNRTHERALEMAKFFQGTPVEYSRFIGMLPEMDIVITSSGAPHFILTRDDMQR
                     VISARRNKPMFLIDIAVPRNIDPAVNGIDNVFLYDIDDLQEVVNSNLHERRKEAERAE
                     SLVLEEVERMMARLNVAEVAPDIVQIQAQLEQIRLAEMEKVRRKYGPFTEKQEEAMEA
                     LTQAIMNKVAHAPISELRNHAGKPDGAHVVAAIRKVFHLQER"
BASE COUNT         1060 a         1603 c         2190 g         1111 t
ORIGIN      
        1 ggcgcggccg taagtcgcgg cgactaagtc atcgccatga atgactaagt cgcggatgga
       61 agttacgggc atgttgagct ggagcgagct ccagtggtcg ccttcgtcga aggagacgta
      121 agcggcgctt tcggttccgg cgtagagcag gcctttgcgc tgcggatctt cgcggacaac
      181 gcgggcgaaa tcaccgtcgg cgaacccgct ggagatctcc tgccacgttt tgccggcgtc
      241 gtgagtgcgg tagaagtgcg ctttgaagtc gttctcttcg tggcgatcga tggcggcgta
      301 ggcggtgttg gcgtcgaagt gggaggcctc gatgatgctg atctgcgtgt tggcggcgag
      361 gccggcgggc gaaacgtcct gccaatgggc gccgttgtct ttcgtgagtt ggatgacacc
      421 gttattggag ccgacccaga tctcacccgc ggcgaccggc gaagggcaga aggcgttgat
      481 ggccgcgcgg ttattgggat tgatggtctc ctgctgttcc tgcgcgcgcg gccgaacgag
      541 ttgcgcggcg ccgggcgagg cgggcgtggt ggcggccgat tcggtcaccg ccggagcctc
      601 tttacggacg gttaaatctg ggctgatggg cttccagtgg acgccgccat cgcgggtttc
      661 gagcagcatt tgcgcgccca ggtaaagcac gtgtccatcc tgcggagaga aggcgagcgg
      721 cggattggtg gccatgcggt actcgttggc ggcgccgcgg ccaatgctgg gcgaaactgt
      781 ggagacctga cgattggtgc gatcgatgcg cacgaggcca cggctgggac ctccggcgta
      841 gacgagattg ttgttgagcg gatcgggcac gatgtagccg aactcgtaac cgccgaccgg
      901 gtcccaatcc atgaaagtga tggcgccgta atcaccgcgg ctgagggtgc cgacggagcc
      961 agaatcctgc tgcgttccgt aaacccaata cgggtagcga ttgtcggtgg agagatggta
     1021 gatctggccg gtgggttggt tgtaccaact gctccaggat ttgccgccgt ccatcgaaat
     1081 ggcgccgccc tggtcgctgg ccatcatcat gcggttggaa tcgagcgggt cgatccaaag
     1141 agcgtggttg tcgtcgccgc cgggcgcgcc tttgtaggag ctgaaggttt ggccgccgtc
     1201 gtcggagcgg tagagcgaag tctgtgcgac gtagacgact ttcggattgc gcgggtcaag
     1261 aaagacgcgg ctgaaatagc cgctgccggt gatgcgcgtg tcgggcgtgc tcttggccca
     1321 atgcgcgccg gcgtcggtgg atttgtagag gcctccgcca cccgctccgc cacccgctcc
     1381 gccggctccg cccgcgacaa tcgcgaatac cgtttgacca gtggcatcgg ttgcaacgcc
     1441 gatgcgccca agccggccgg tgggcagtcc atcggtgact tgcgtccacg tgtcgccacc
     1501 atcagtcgtc ttgaagacgc ccgcagtgcc tgtcgattcg atagctgcgc gagcgccggg
     1561 gcgcgtgtag tgctccatga tggcggcgta accgaccttg ggattgctgg gggcgaaggc
     1621 gagatcgact gcgccggtgg tgtcgtcttt ggccagagtc tggcgccagg tcttgccgcc
     1681 atcggtggtc ttgaagatgc cgcgctgctt gttcttggag taagtacggc cgagcgcggc
     1741 gacgacgaca gtgttgggat cgtgcggatc gatgaagaga ttaccgatgt gttcggtctc
     1801 ttcgaggccg atgtgctgcc atgttttgcc ggcgtcaacg gatttgtaga cgccgtcgcc
     1861 gaagttgacg gagccgccca ccatgctgac atcgccagtt ccgacgtaga tgatgttggg
     1921 atcggagagc gccacggata cagcgccgat ggaggaagtg gagcggacct gatcgaagat
     1981 gggcgtccag atgacgccgc catcgatggt tttccagagg ccgccgccgg gcgtgcccat
     2041 atagtagatg gcggcgttgc cggggatgcc ggcgaccgcg gttacacgtc ccgcgcgaaa
     2101 ggggccgatc tgacgccaac gcatcgcctg atagagcttg ggcgtgtacg tctgggcgta
     2161 gaggccgaat gcgcctgcgg acagggcaag cacagccaac gagacgtata gtttgttacg
     2221 catgtaccga ttttccatga aagatagtga agctacaagg ggcgctgtgg ttgagcggcg
     2281 agaatggcgt cgaccagccc atcgacggtg aattcgcgag cttcgatggg gacctcgatg
     2341 ccgaggtcgc gcgcggtgcg cgtggtgatg gggccgatcg aagccgcggc gatgccgcgg
     2401 agggactcgg cgccggcagc ggcgaccaga ttttgaacgg tggaggaact ggtgaaggtg
     2461 atgatgccgg gcttgcgcgc ggcggagaag acttcgcggg cgcgttccgc gagtccggcg
     2521 ggcgcgacgg tgcgataggc ttcgacgata tcgacctggg cgccgcggcg cgtgagttca
     2581 gcggggacaa ggtcgcgagc tacggcggcg cgcggcaaga gcacgcgacg gccggtgaga
     2641 tcgtgagcgg cgaaggcttc gatgaggctc tcggcaacgt actctttgcc catgagatcg
     2701 actttgaggt gcagggcttc gacggcggcg cgcgtggcgg gaccgatggc gcagatgcgg
     2761 gcgcggagtt tgcgcaggtc gatggcggag cggtcgagcc gatcgatgaa atgcttcacg
     2821 ccgttgacgc tggtgaagat gagccagtcg taggaatcga gtgcggcgat ggcggcgccg
     2881 aggggcgcgg ggtcggcggg cggctgaatt tcgatggcgg ggagttcgac gatatcggcg
     2941 ccgaaggccg caagtttcga accgagcgca tcggcttggg cgcgagcgcg agtgacaacg
     3001 atgcggcaac cgaagagagg aaggcgctca aaccaatcga gcttcgggcg caggcggacc
     3061 acttcgccga cgacgatggt cgctggcggt ttgatgtggc tggactcgat cagacgtggc
     3121 agcgattcga gcgtaccgac gatggtttcc tgatcggcgc gggtggccca acggacggcc
     3181 atggcgggcg tatcggcagc gcgaccacgg acgatgagtt cgtgggcgat ggcaggaaac
     3241 gtggtgaggc ccataaagac gaccagggtt tccgacatgc cgacgcggtc ccaatcgatc
     3301 tgatcgacgg catggccggt gacgaaggtg acggcggagg tgtgctcgcg gtgcgtgagc
     3361 gggacgccgg tgtaggctgc gatgccgagg ggcgtggtga cgccggggac gacctcgaac
     3421 gggatgcctg cgtcggcgag ggcttcggcc tcttccccgc cccggccgaa gatgaacggg
     3481 tcgccacctt tgagacggac aacggtgaag ccgcggcggg cgcggtcgat gagcatggcg
     3541 cagatttcct cttgcgagta ggcgtgcgcg gactttttct tcccgacgta gaggcgctcg
     3601 gcagcgggag gagcgagatc gagcaggcta tcgctggcga ggtgatcgta gagaacggaa
     3661 tcggcctgtt cgaggacgcg gcggcctttg acggtgatca gaccgggatc gcccggaccc
     3721 gcgccgacga ggtagacttt cggcgatcgc ggattagaca tgcacttcga gtagcgcttg
     3781 ggcgccgcgg gagagcacgt cggcgccaag tgaggcgcca agctcttcgg ccgaatgtga
     3841 ggggccggag cgatcgccgg tgacaacctg cgagccatcg gcagcgatga cgaggccgcg
     3901 aatgcgaagc gtttcgccat cgacttcggc gtgcgcgccg atgggaacct ggcagccgcc
     3961 gcccaacgag cgcaggaaag cgcgctcggc agttaccgcg gcgcgagtgg cggcgtgatc
     4021 gagcgcggcg caggcgtcgc gaccagggcc ggtggtgcaa gtttcgactg cgagtgcgcc
     4081 ttgaccgaca gcagggcaca tcacatcggg cggaagcact tcggcgatgc gattctccca
     4141 accgaggcgg cgcaggcctg cggaagcgag cacgatggat gagtactggc cttcatcaag
     4201 cttgcgcagg cgcgtatcga ggttgccgcg aatggattcg atgcgcagat tgggatgcag
     4261 gcggagcagc tgcgcagcgc ggcgcaggga actggtgccg atgacgtcgc cagggttgag
     4321 ttcagcgagt tttttgccta cgacggcatc acgcggatct tcgcggaccg gaatggcagc
     4381 gagcgcgagg ccatcgggaa gcgtagtagg caggtctttg aggctgtgga cggcgagatg
     4441 ggcgcggcca tcgaggaggg cttcttcgat ttctttggtg aagaggcctt tgccgccggt
     4501 ggcggcgccg agttgcgcaa gcgaggcttc agcgaactta tcgccggtgg ttttgatgat
     4561 ttcgatttgg cattcgtggc cgagttcaat gagtcgcgct tcgatccagc gggcctgcca
     4621 cagcgcgagt tgcgaaccgc gcgatgcaat agtcagcttc atcgttcttg taagtggaat
     4681 acctttcgaa tcgccgcgac gacgtgcgca ccatcgggtt tgccggcgtg gttgcgcagt
     4741 tcggagatgg gcgcgtgcgc cactttgttc atgatggcct gcgtgagggc ctccatcgcc
     4801 tcttcctgct tttcggtaaa cggaccgtat ttgcggcgga ccttttccat ttcggcgagg
     4861 cggatctgtt cgagttgcgc ctgaatttgg acgatatcgg gggcgacttc ggcgacgttg
     4921 aggcgcgcca tcatgcgttc gacctcttcg agaacgaggc tttcggcgcg ttcggcctct
     4981 ttgcggcgct cgtggaggtt ggaattgacc acctcctgca gatcgtcgat gtcgtagagg
     5041 aaaacgttgt cgatgccgtt cacggcgggg tcgatattgc gcggaacggc gatatcgatc
     5101 aggaacatcg gtttgttacg gcgcgcggag atgacgcgct gcatgtcgtc gcgcgtgaga
     5161 ataaaatgcg gcgcgccgga ggacgtgatg acgatgtcca tttcaggcag catgccgatg
     5221 aagcgcgaat attcgacggg cgtgccttga aaaaacttcg ccatctcgag agcgcgctcg
     5281 tgcgtgcggt tggtgacgaa gatgccggtg gcgccggagc ggcgaaggtg gcgcgcggcg
     5341 agttcggaca ttttgcccgc gcccacgatc atcacatttt tgcctttgag cgagccgaag
     5401 atcttgcgag cgagttcgac agcggcgtaa ctgatggaga cggccatctg gccgatgccg
     5461 gtttcggagc ggacgcgctt ggcaacggaa aacgcgcggg agacaagcga atcgagccag
     5521 ccggtgacag ctccgcactc tttggcagcg ccgtaggcgg ttttcagttg acctaagatt
     5581 tggggctcgc caacgaccat tgaatcaaga ctggaggcga cgcggaacag gtgatgaatg
     5641 gccgcgcggc cttcgtggcg gtagagctgg ccgccgagtt tgcgtgcgtc gagacctttg
     5701 tgggcggcga gaaagccgtc gacgagagag gcggggtcgg tggagtcttc ggaggcgatg
     5761 gtgatctcca cgcggttgca ggtggaaaga atcatcgctt cgcatacgcc agattgcgat
     5821 ttgagatcgc cgagggcgga cgggatgacg tcttcaccga aggccagcga ttcgcgaacg
     5881 gcgagaggag ccgatttgtg attgaccccg aagatggcga gcttcattgg cccagcaaag
     5941 ctcccaaacg cgagtgcgcc accc
//
LOCUS       DASZMC010000619         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3555283,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000619 DASZMC010000000
VERSION     DASZMC010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555283"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..933)
                     /locus_tag="VG274_12580"
     CDS             complement(172..933)
                     /locus_tag="VG274_12580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE07542.1"
                     /translation="MPITRAQTGDEQRRTLILTGASRGIGHATVKRFASAGWRVITCS
                     RHAFPENCPWAAGPEDHIQVDLGDAGDTLRAVREMRERLDDGKLHALVNNAGISPKGP
                     DERRLGVLASGTDVWRQVFEVNLFATIWLASGLIEELTSAKGAIVNVTSIAGGRVHPF
                     AGAAYATSKAALAALTREMAADFGPLGVRVNAIAPGEIDTAILSPGTEKIVESIPLRR
                     LGAPEEVAKAIYYLCTEQSSYVTGAELHINGGQHV"
     gene            complement(981..1856)
                     /locus_tag="VG274_12585"
     CDS             complement(981..1856)
                     /locus_tag="VG274_12585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWE07543.1"
                     /translation="MQRAAFAAVAISALLVGLKATAYVITDSVAMMAALADSALDLFA
                     SSINLLAINQSLTPADREHRFGHGKAEPLAGLAQGAFIAGSATFLVVESIGRIVSPHA
                     VGHAAIGLVVMAFSVMAVLGLVLYQRVAVARSGSLAIQADRLHYLGDLLTNIGVIVGI
                     ALATRFGILIADPIVGLGVAMVLTASAWQVVRQSYDQLMDHELPEGERDRIKAIVLAH
                     RDVRNLHDLRTRAAGISKFVQLHIELDPAISLTRAHEVSDAVEAEILAAYPDAEVIIH
                     QDPAGVEKPPPLARS"
     gene            complement(1901..2527)
                     /gene="pdxH"
                     /locus_tag="VG274_12590"
     CDS             complement(1901..2527)
                     /gene="pdxH"
                     /locus_tag="VG274_12590"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HWE07544.1"
                     /translation="MSDIGGPLDDGGIATQPDPIAHFAGWFAEAVKNEPHEPEAMALA
                     SVDAEGRPNVRMVLLKGADADGFAFYTNLESAKGRELGSQSHVALCFHWKSLRRQVRV
                     RGPASPVGAAEADAYFATRPKDSQIGAWASAQSRPMEGRFAFEREIARYAAKFALTKV
                     PRPPFWSGFRVTPLEVEFWRDRPFRLHDRLLYRREAPDAPWRTERLYP"
     gene            2686..>3356
                     /locus_tag="VG274_12595"
     CDS             2686..>3356
                     /locus_tag="VG274_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWE07545.1"
                     /translation="MRDPYEVLGVSKTASEAEIKKAFRSLAKKHHPDTRGGDSAAKKR
                     FQEISAAYDIVGDKEKRTKFDQGMIDASGNPRGFEPGAHGFRGSPFGRGEGPGNFHFA
                     WQDVDAAEGGFQPEDILADILGGGGRRRRQARKGEDFSFALTIGFEEAARGGTRRIVL
                     PDGREVDVRIPVGVRDGQQIRLKGQGGPGRENGPAGDLLLTLTVAAHPFLTREGNDLR
                     MDLPVT"
BASE COUNT          642 a         1073 c         1071 g          570 t
ORIGIN      
        1 ggacgcagga aaatagattg cgcgcgaagc gcgcctgaat ttcccccgcg tccttccgcc
       61 tacgccaagg cttcggcgga ccaaatgagc cgcctgaccc gccgtagctt tagcgaaggc
      121 gggccgcgca tcctctgtgt cctttgcgct aaatccttgt ggttacacga gctagacgtg
      181 ctggccgccg ttgatgtgca gttcggcgcc ggtgacgtag ctcgattgct ccgtacaaag
      241 ataatagatc gccttggcga cttcctcggg cgcgcccagc ctccgcaacg gaatggattc
      301 gacgattttc tctgttcccg gcgaaaggat tgcggtgtcg atctcgccgg gtgcgatcgc
      361 gttcacccga acgccgagcg ggccgaaatc ggcggccatc tcgcgcgtca atgcggcaag
      421 cgccgccttg gacgtcgcat aggccgcgcc ggcgaaagga tggacacggc ctcccgcgat
      481 cgaggtcacg ttgacgattg cgcctttcgc agacgtcagc tcctcgatca ggccactggc
      541 gagccagatg gtcgcaaaga gattgacctc gaacacctgc cgccagacgt ctgtgccgct
      601 cgcgagaacg ccgagccggc gctcgtccgg ccccttgggc gagatgcccg cattgttcac
      661 gagcgcgtgg agcttgccat cgtccaggcg ctcacgcatt tcgcgcacag cgcgcaacgt
      721 gtcgccggcg tcgccgagat cgacctggat atgatcctcc gggcctgcag cccacggaca
      781 gttttcgggg aacgcgtgac gcgagcaggt gatcacccgc cagcccgcac tggcgaaacg
      841 cttcacggtt gcatgcccga tgccgcggct cgcaccggtg aggatcagcg tgcggcgctg
      901 ttcgtcgccg gtttgcgcgc gtgtgatggg catcggcacc cactgtagca actcgctgcc
      961 gctcttgcga acgctgcgat ttacgaccgc gcgagcggcg gaggtttctc cacgccggcc
     1021 gggtcctgat ggatgatcac ttccgcatcg ggataggccg caaggatctc cgcctccacg
     1081 gcatcgctca cttcgtgcgc ccgcgtaagg ctgatggccg gatcaagctc gatatgcagc
     1141 tgcacgaatt tcgagatgcc tgcggcgcgc gtgcggagat cgtggaggtt gcggacatct
     1201 cggtgagcga gcacgatggc cttgatccgg tctcgttcgc cctcgggcaa ttcgtgatcc
     1261 atgagctgat cgtagctctg ccgcacgact tgccaggcgc tcgccgtcag caccatggca
     1321 acgcccaatc cgacaatagg atcggcgatc aggatgccga aacgggtcgc cagcgcgatg
     1381 cccacgatca cgccgatatt cgtcagcaga tcgcccagat agtgcagacg gtcggcctgg
     1441 atggcgagag accccgagcg cgcaaccgcg acgcgctgat agaggacgag cccaagcacc
     1501 gccatcacag agaacgccat gacaacgagc ccgatcgcgg cgtgcccgac cgcatgtggc
     1561 gaaacgatcc ggccgattga ttcgaccacc agaaatgtgg ccgagcccgc gatgaacgcc
     1621 ccttgcgcca gccccgccag cggctcggcc ttgccgtggc cgaaccggtg ctcgcggtcc
     1681 gcaggcgtaa gcgactggtt gatcgcaagc aggttgatcg aagacgcaaa caggtcgagc
     1741 gcggaatcgg cgagcgccgc catcatcgcg accgaatccg tgatcacgta ggcggtcgcc
     1801 ttaaggccaa ccagcagggc ggatattgcg actgcggcga aggccgcgcg ctgcatgatc
     1861 cgcgattttt cgcttcggtc caagattggc gaaacaactg tcacggatac agccgctcgg
     1921 ttcgccacgg cgcgtcaggc gcttcccggc gatagagaag ccggtcatgc agccggaatg
     1981 gccggtcgcg ccagaactca acctccagcg gagtcacgcg aaatcctgac cagaagggcg
     2041 ggcgcgggac cttggtcagc gcaaattttg cggcatatct ggcgatttct cgctcgaacg
     2101 cgaagcgtcc ttccattggg cgcgactgcg cggacgccca ggcgccgatc tggctatcct
     2161 tgggccttgt cgcaaaataa gcatcggctt cggctgcgcc gaccgggctt gccggacctc
     2221 gcacacgcac ctggcggcgg agcgacttcc agtggaagca cagcgcgacg tgagattgag
     2281 acccgagttc cctgcccttc gcgctctcaa ggttcgtgta gaacgcgaaa ccatctgcgt
     2341 cggcgccttt cagcaaaacc atgcgcacgt tcggccgccc ttcggcgtcc acgctggcga
     2401 gcgccatcgc ttcgggttca tgaggctcgt ttttgactgc ctcggcgaac cagcccgcga
     2461 aatgcgctat cgggtcaggc tgcgtggcga tgccgccgtc atccagggga ccgccgatgt
     2521 cgctcatatg ccgccgcata gcatcttcgt ccgggcgcag aaagccgagc taccttcgcg
     2581 ccaccggcct ttccttacta tattgaaccg gaccccgact cgacgacgcg gcacggaagc
     2641 gcgagcgagt ctgtgagcga tcgagcaaag tgcggccaaa aaaatatgcg cgacccctac
     2701 gaagtgcttg gtgtttcgaa aacggcaagc gaggccgaaa tcaagaaggc ctttcgtagc
     2761 ctcgccaaga aacatcatcc cgatacgcgt ggcggggaca gcgccgcaaa gaagaggttt
     2821 caggaaatca gcgccgcgta cgacattgtg ggagacaagg agaagcggac gaaattcgac
     2881 cagggcatga tcgacgccag cggaaatccg cgcggattcg agcccggcgc tcatggcttt
     2941 cgcggcagtc cgttcgggcg cggtgaaggc cccggcaatt ttcacttcgc ctggcaggac
     3001 gtggacgcag cggaaggcgg atttcagccc gaggacatcc tggccgacat tcttggcgga
     3061 gggggaaggc gaaggcggca ggcgcgaaag ggcgaggatt tttcattcgc gctcaccatt
     3121 ggcttcgagg aggccgcgcg cggcggcaca agacgaatcg ttttgccgga cggacgcgaa
     3181 gtggatgtgc gcattccggt cggcgtgcgc gacggccagc agatccggct gaaaggacaa
     3241 ggcgggccgg ggcgcgaaaa cggacccgct ggcgacctcc tgctcacgct cacggtcgcc
     3301 gcccatccct tcctgacccg ggaaggcaac gatctcagga tggacctgcc tgtcac
//
LOCUS       DASZMC010000620         5386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3555402,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000620 DASZMC010000000
VERSION     DASZMC010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555402"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..907)
                     /locus_tag="VG274_12600"
     CDS             complement(167..907)
                     /locus_tag="VG274_12600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWE07546.1"
                     /translation="MSDASPGGDRIAKVIARAGICSRREAEKLIADGRVALDGETVKT
                     PAKRVGERQIVSVDGKPLSEPERTRLWRYHKPVGLVTTHRDPEGRPTVFQALPKSMPR
                     VVSVGRLDVNSEGLLLLTNDGALARKLELPAAGWTRQYRVRLFGKVSQNDLDRLAQGI
                     TIEGVRYGPVIADLERSKGMYSWASVGIKEGKNREVKRVMEHLGLKVARLIRIRFGPF
                     HLGHLEPGAVDEIPAKIWKSATENEAPK"
     gene            969..1421
                     /locus_tag="VG274_12605"
     CDS             969..1421
                     /locus_tag="VG274_12605"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWE07547.1"
                     /translation="MSEIDHMALALAEAHAAAERGEVPVGAVLAAADGQVLARNGNRI
                     VERRDPTAHAEMLVLREGAQKLDNERLTGTALFVTLEPCAMCAGAVSLARVEKLVFAA
                     ADPKGGAVLHGPRYFEQPTCHHRPSIEWGEQAEESAGLLKRFFLVRRA"
     gene            1519..1821
                     /locus_tag="VG274_12610"
     CDS             1519..1821
                     /locus_tag="VG274_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE07548.1"
                     /translation="MALIVYGASLSPFVRKVRVMLAEKGLDYKIEYVNPFSQPPEFAD
                     ISPLKRIPVLRDTDRPEPNTLPDSSVICDYIDRKWPTPALYPAEPFERAQALWFEE"
     gene            1806..2186
                     /locus_tag="VG274_12615"
     CDS             1806..2186
                     /locus_tag="VG274_12615"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE07549.1"
                     /translation="MVRGIADSLVAQSIGLGLFFERVVKKLIGGECDETVVTGTIRDK
                     LPPIFDYLDKTIGARRYLVGDKFSIADIAVASMFVNYDHAGETIDSKCWSNLSRYVAE
                     IHARPSFGACVDGERRFLDQQRAA"
     gene            complement(2196..2915)
                     /locus_tag="VG274_12620"
     CDS             complement(2196..2915)
                     /locus_tag="VG274_12620"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWE07550.1"
                     /translation="MLPLIVDPHAWASLATLTALEIVLGVDNIIFLSIAAARLAPVRR
                     TSARRIGLLLALVMRIALLTGIVWIVSLSKPVVTLGGLALSWRDLIFAAGGLFLLTKG
                     TREIHGLVEGDEEATRRTAASFLSVIVQIALFDLVFSIDSVVTAVGMAEHLSIMIAAV
                     ILAMLVMLAASGPASDFVNRHPTVKMLALSFLLLIGTALIADAAHFHIPRGYLYFAVA
                     FSALVEALNQYAARASRKRAG"
     gene            complement(2928..3542)
                     /locus_tag="VG274_12625"
     CDS             complement(2928..3542)
                     /locus_tag="VG274_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWE07551.1"
                     /translation="MWTGIALIVCGLACFAIDRRAVHAFHAAVGRPIERVLHAATDWA
                     KGSHWMLTAAITFAVVRLAEWRFGPGPLFNLAADASLAFLASLALASLILHTIKILLG
                     RRRPRDELELDLYGFRPFAFDLRSDSFPSGHALTIFCVAVVLSAAVPRLTVLWFAIAI
                     FLALTRALLNSHFLSDVMVGAGTALITTREVLIYFFPALAHPWF"
     gene            3810..5054
                     /locus_tag="VG274_12630"
     CDS             3810..5054
                     /locus_tag="VG274_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWE07552.1"
                     /translation="MNFDYSPRQKEWMARVREFMDRHVCPAEETYAAQMEEATRNGNR
                     WIVVPVVEELKAKAKRQGLWNLFLPDSERGAGLSNLEYAPLAEMMGRVGFASEVFNCS
                     APDTGNMEVLERYGSPAQQEKWLKPLLEGEIRSAFIMTEPAVASSDATNIATRIERQG
                     DHYVINGRKWWSSGVGDPRCRIMIVMGKTDPESDKYRQQSQVLVPMDAPGVKIERMLP
                     VFGFDDAPHGHGQVLFENVKVPVDNVILGEGRGFEIAQGRLGPGRIHHCMRAIGVAER
                     ALEQMVRRLVSREAFGKKIAEHSVWEQRVAEARTNIEMCRLLTLKAADLMDKAGNKVA
                     RTEIAMIKVAAPRMALKIVDDAIQAWGGAGVTSDSGLARMYASLRTLRLADGPDEVHN
                     RTIARLEYGKYMSGVVAQRSHA"
     gene            5115..>5386
                     /locus_tag="VG274_12635"
     CDS             5115..>5386
                     /locus_tag="VG274_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWE07553.1"
                     /translation="MLKLYQANLSPYAARVRILAAAKGLKIEFLPPAGGSLKSAEYLA
                     INPLGRIPCLDHDGAVIPESETICEYLEDAFPTPTLRPVDPEARARA"
BASE COUNT         1089 a         1627 c         1733 g          937 t
ORIGIN      
        1 ggacgcagga aaatagattg cgcgcgaagc gcgcctgaat ttcccccgcg tccttccgcc
       61 tacgccaagg cttcggcgga ccaaatgagc cgcctgaccc gccgtagctt tagcgaaggc
      121 gggccgcgca tcctctgcgt cctctgcgct aaattttttt tgttttttat ttcggggcct
      181 cgttttcggt tgcggacttc cagatttttg ccgggatctc gtcgacggcg ccgggttcga
      241 ggtggccgag gtggaagggt ccgaagcgaa tgcggatgag gcgcgcgacc ttgaggccga
      301 gatgctccat cacacgcttc acttcgcggt tcttgccttc cttgatgccg acgctcgccc
      361 atgaatacat gcccttgctg cgctccaggt cggcgatgac cggcccgtaa cgcactccct
      421 cgatcgtgat tccttgcgcg agacggtcga gatcgttctg cgacactttg ccgaagagcc
      481 gcacgcggta ctggcgcgtc cagcccgctg ccggcagttc gagctttcgc gccagcgcgc
      541 cgtcattggt caaaagcagc aggccttccg aattgacgtc gaggcggccc accgacacca
      601 cgcgcggcat gctcttcggc aaggcctgaa aaaccgtggg ccggccttcg ggatcgcggt
      661 gcgtggtgac cagtcccacc ggcttgtgat agcgccaaag ccgcgtgcgc tcgggctcgc
      721 tcagtggctt gccgtcgacg gatacaatct ggcgttcgcc cactcttttg gccggcgttt
      781 tcaccgtctc gccgtcaagc gcgacgcgcc cgtccgcgat gagtttttcc gcctcgcggc
      841 gcgagcagat gccggcccgc gcgatcacct ttgcaatgcg gtcgccaccg gggctggcgt
      901 cggacacgag cggatgcctt tattggcgaa gagttgggcg atagcgaggc agtatcacac
      961 gcacagccat gagtgaaatc gatcatatgg cgctggcgct cgcggaggcg cacgcggctg
     1021 cggaacgcgg cgaggttccg gtcggcgctg tgctcgcggc ggcggacggg caggtgttgg
     1081 cgcgcaacgg caaccgcatc gtcgagcggc gcgaccccac cgcgcatgcg gaaatgctgg
     1141 tgctgcgcga aggcgcgcag aagctcgaca atgagcgtct gacgggcacg gccctgttcg
     1201 tgacactcga accctgcgcc atgtgcgccg gcgcggtgag cctcgcccgc gtcgagaagc
     1261 ttgtcttcgc cgcagccgac cccaagggcg gcgcggtgct ccacggcccg cgctatttcg
     1321 aacagccgac ctgtcatcat cgcccgtcaa tagagtgggg tgagcaagcc gaagaatccg
     1381 ctggcttgct caaaaggttt tttctcgtgc ggcgcgcctg actctcggca ttggccgcgg
     1441 tcttgaaaac ttcgcttgag actccggcgt caagcctgac aagctgtccg ttccgggatc
     1501 gcaatcgggg agacgaaaat ggcattgatc gtttacggcg cttcgctttc accctttgtg
     1561 cgcaaggtgc gcgtgatgct ggcggagaag ggactcgact acaaaatcga gtacgtcaat
     1621 ccgttttcgc agccgccgga attcgccgat atcagtccgc tgaagcgcat tcccgtcctg
     1681 cgcgataccg acaggccgga gcccaatacg ctccccgatt cctcggtgat ctgcgactac
     1741 atcgaccgca aatggcccac acccgcactc tatcccgcgg aaccattcga gcgcgcgcag
     1801 gcgctatggt tcgaggaata gccgactcgc tggtcgcaca gagcatcggc ctcggcctgt
     1861 ttttcgaacg ggtggtcaag aaactgatcg gcggggagtg cgacgagacg gtcgtaaccg
     1921 gaacgatccg cgacaagctg ccgcccattt ttgactatct tgacaaaacg atcggtgcac
     1981 gccggtatct ggttggcgac aaattctcca tcgccgatat cgcggtggcg agcatgttcg
     2041 tcaattacga ccatgccggc gagacgatcg attcgaaatg ctggtcgaat ctgtcccgct
     2101 acgttgccga gatccatgcg cgcccgtcat tcggggcctg cgtcgacggc gagcggcgtt
     2161 tcctcgatca gcaacgcgcc gcgtgaaccg ggctctcagc ccgctcgttt tcggcttgcc
     2221 cgcgccgcgt actggttgag cgcttcgacg agcgccgaga acgcgaccgc gaagtagagg
     2281 tacccgcggg ggatatggaa atgcgccgcg tcggcgatga gcgcggtgcc gatcagcagg
     2341 agaaaactga gcgccaacat cttcacggtc gggtgcctgt tgacgaagtc ggacgcaggc
     2401 ccggaggccg cgagcatcac cagcatggcg aggatgaccg cggcaatcat gatggagaga
     2461 tgctccgcca tgcccacggc ggtcacgacg gagtcgatcg agaagacgag atcgaagaga
     2521 gcgatctgaa cgatcacgct caagaacgaa gcggcggtcc ggcgcgtcgc ctcttcgtcg
     2581 ccctcgacga gaccgtgaat ttcgcgcgtg cccttggtga gaaggaaaag gccgccggcg
     2641 gcgaagatca gatcgcgcca tgatagtgca agccccccca gagtcaccac gggctttgac
     2701 agcgagacga tccagacaat tcccgtgagc agcgcaatgc gcatgacaag cgccagcaaa
     2761 aggccgatgc ggcgggcgga cgtccggcgg accggtgcga gacgggcggc ggcgatcgac
     2821 aggaaaatga tgttgtcgac gccgagcacg atctcgagcg cggtcaatgt cgcgaggctc
     2881 gcccaggcgt gaggatcgac gatgagagga agcattgcct gccttgttca gaaccacgga
     2941 tgggcgagag cggggaagaa atagatgagt acctcccgcg tcgtgatcag cgcggttccc
     3001 gccccaacca tcacgtcgct caagaaatgc gaattcagaa gggcgcgtgt cagcgcaaga
     3061 aatatcgcga ttgcgaacca gagaacggtc agacgtggaa ccgccgcaga gagcacgacg
     3121 gcgacgcaga atatcgtcag cgcgtgaccg gacgggaacg aatcgctgcg caaatcgaag
     3181 gcaaacgggc gaaagccgta cagatccagc tccagctcgt cgcgtggacg ccgtcgtccg
     3241 agcaggatct tgatcgtgtg gaggatgaga ctcgcgagcg caagactggc aagaaaggca
     3301 agacttgcat cggcagccag gttgaacagc gggccgggtc caaacctcca ctccgcgaga
     3361 cgcaccacag cgaacgtgat tgcggctgtc agcatccaat gcgaaccttt cgcccaatcg
     3421 gtggccgcgt gcagaacgcg ctcgatcggg cgtccgacgg cggcgtggaa tgcatgcacc
     3481 gcgcgccggt cgatggcgaa gcaggccagg ccgcacacaa tgagggcaat tccggtccac
     3541 atcagcacga ttgttgcttg ccgcgccagg atcaatctgt caaaccgtac ccaaacacgt
     3601 catccccgcg ccaccggtcc gcgcgaagcg cggccggagc gtaaactccg gcggggaccc
     3661 aagggcaaac gacgtgcgtt cgccccggga tcccgctcca gcgggaatga caaattaggt
     3721 ttgtgataca atttgatcag accctgcttt gagttcattc ccgcgctcgc aatcggcgag
     3781 tagtgcctca ccgcttggga gatcagctga tgaattttga ctattcgccg cgtcagaagg
     3841 aatggatggc gcgcgttcgt gaattcatgg atcggcacgt ctgtccggcg gaggaaacct
     3901 acgcggcgca gatggaggag gcgacgagga acggcaatcg gtggattgtc gtgccggtcg
     3961 tcgaggagct gaaagcaaag gcgaaacggc agggattgtg gaacctgttt ttgcccgaca
     4021 gcgagcgggg cgccggactc agcaatctgg aatatgcgcc gctggcggaa atgatgggac
     4081 gggtgggctt tgcgtccgaa gtgttcaatt gttccgcgcc ggacaccgga aacatggaag
     4141 tgctggagcg ttacggcagc cccgcgcagc aggagaaatg gctgaagcca ttgctggaag
     4201 gcgaaatccg ctcggcgttc atcatgacgg aacctgcggt cgcctcgtcg gacgcgacca
     4261 acatcgcaac gcggatcgag cgccaaggcg atcattacgt catcaatggc cgcaaatggt
     4321 ggtcgtcggg agtgggcgac ccgcgttgca ggatcatgat cgtgatgggg aagacggatc
     4381 cggaatccga caagtaccgg caacaatcgc aggttctcgt gccgatggac gcgcccgggg
     4441 tgaagatcga gcgtatgctt cccgtattcg gtttcgacga cgcaccgcac gggcacggcc
     4501 aggtgctatt cgaaaacgtg aaagtgcccg tcgacaacgt gattctcggc gaagggcgcg
     4561 gattcgagat tgcccagggg cgcctcggcc ccggacgcat ccaccattgc atgcgcgcga
     4621 tcggcgtggc cgaacgcgcg ctcgagcaga tggtccgccg gctggtgtcg cgcgaagctt
     4681 tcggaaagaa gattgcagag cattcggtgt gggagcagcg cgttgccgaa gcccggacaa
     4741 atatcgagat gtgccgcctt ctgacgctca aggcggcaga cttgatggac aaggcgggca
     4801 acaaggtagc gcgcacggaa atcgccatga tcaaggtcgc ggcgccgcga atggcgctca
     4861 agatcgtcga cgacgcgatc caagcgtggg gaggcgcggg cgtcacgagc gattctggcc
     4921 tcgcccgcat gtatgcgagt ctgcgcactc tcaggctcgc cgacggcccc gacgaagtcc
     4981 acaaccgaac cattgcccgc ctggaatacg gcaagtacat gtcgggtgtc gtcgcgcaga
     5041 gatcacacgc gtgacacaaa gagtctgaat accgtagacg actcgcacca tccgccttcc
     5101 gtgtcggagt aaccatgctc aagctgtatc aggccaacct gtcgccctat gcggcgcgcg
     5161 tgcgcattct ggccgccgca aagggattga aaatcgaatt cctgccgccc gcgggaggca
     5221 gcctgaaatc ggcggagtat ctggccatca atcccctggg caggattcca tgcctcgatc
     5281 acgacggcgc ggtcattccg gaatccgaaa ccatctgcga atatctcgaa gacgcttttc
     5341 cgacgccgac gcttcgaccc gtcgatccgg aggcccgcgc gagggc
//
LOCUS       DASZMC010000621         5689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_824281,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000621 DASZMC010000000
VERSION     DASZMC010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824281"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VG274_12640"
     CDS             <1..472
                     /locus_tag="VG274_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07554.1"
                     /translation="ADRSSTFTPTIKYGKGFGDLPDEMALLRPIAITGIVGYAIAGTN
                     TNSIAWAGALEYSLLYLQNNVRDQGFSNFVARLTPIVEYSFTSPVDADGGVTTGTFNP
                     GLIWSGQYTQLAIEAIVPANRASGDNVGVIAQLHFYIDDIFPDTLGKPLFGGKP"
     gene            469..819
                     /gene="copC"
                     /locus_tag="VG274_12645"
     CDS             469..819
                     /gene="copC"
                     /locus_tag="VG274_12645"
                     /inference="COORDINATES: protein motif:HMM:NF033814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HWE07555.1"
                     /translation="MNARSFLACLAIMGFAAPAFAHAFLEHAAPGAGETLATAPKQVA
                     LAFSEQLEPAFSGVTVADSAGSDVESGRAVVAGASMSVAVKPLAPGTYRVTWHAVSVD
                     THRTEGAYSFTVKP"
     gene            816..1688
                     /gene="copD"
                     /locus_tag="VG274_12650"
     CDS             816..1688
                     /gene="copD"
                     /locus_tag="VG274_12650"
                     /inference="COORDINATES: protein motif:HMM:NF033808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HWE07556.1"
                     /translation="MIVFFAAVRAIHFASLMAIFGAGAFLALLRSRLHIEVPAIAARI
                     PFASAATLALITAVASLGLVAGQMSGDWHSALDPTALTAVATGTRFGQVFLWRIGGLA
                     VLWIACILKTPSQNLAPALLAALLLGALGLTSHAAASSGEFGIVRAGNDAAHLLAGGF
                     WLGGLLVLAMLMWRHREAPAALLRPLQLFSAWGTYAIAALVVTGVTNAASILPAAAVS
                     VRSAYARVLLSKVAIASVMIALAAVNRWRLMPALRSGERGAPHDLARSIGAEIALGAA
                     VIAMAAWLGLMPPR"
     gene            1994..2950
                     /locus_tag="VG274_12655"
     CDS             1994..2950
                     /locus_tag="VG274_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWE07557.1"
                     /translation="MKALRFDRFGPPSVLSLQDVEVANPRPGEALIELHAAAINPSDV
                     KNVAGAFHATLPRTPGRDYAGVVFQGDGWKGREVWGSGAGLGVTQDGTHAQYLVVPID
                     WLCEKPAQLSMEQASSVGVPYLAAWSALVDAAEAQAGETVLVTGALGAVGRAATQIAH
                     WKKARVIGGDRSEGASDADAFVNTSKKDLAAEVKALTDGKGADVALDTVGGPMFEPVL
                     KSLRVGGRQVAITSAVNRRVEFDLVDFYHNALRLIGLDTMKLTGPGIAKIMDALRAGF
                     ESGELKPPEIREWPLDKAVEAYAAVEKGGSQTKHVLLPQKGL"
     gene            complement(2957..4591)
                     /locus_tag="VG274_12660"
     CDS             complement(2957..4591)
                     /locus_tag="VG274_12660"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE07558.1"
                     /translation="MATRTQSETQMPPSSPRAKLPGSPFRHKAFAVIWTATVVSNVGW
                     WMYTAASGWLMLSLNSAPLVVSLVQVANSLPMFLFAIPAGALTDIVDKRKFLIAGEIS
                     IAAVATAFAALVWLHLVTPITLLLFAFLVAAGDALTSPAYQAIVPLLVPKTDLPKAVV
                     ANSAGINVSRAVGPALGGALLGVFGIAAPFWINAVSNFGSIGALVWWHEPVRSGARLP
                     PEHLLSAIRSGFRYARYNASLIATLIRAAGFLLFASAYWALLPLVVRNQIGGGPMLYG
                     ILLGAIGASAVGGAFLLPWLNDRLVPDRVAAAGTLGTAAATALYGLAHEGVTALAASL
                     LAGVSWMTMLSVLNISAQYALPEWVRGRGLAIYVTVMFGALSLGSAIWGQAATVLGLG
                     PALFIAAAGCATGIPLLGRWRLQMAKGLDLSPSIHWPVPVMTADFDAHRGPVMVMVTY
                     EIDPRNREQFLEALDQVGRERRRDGAYQWQVFEDPNKKGCFVETFLSDSWLDHLRQHE
                     RVTNADHAQEDAARKFQAGEGPVTTHFIAARPRRRS"
     gene            4860..5285
                     /locus_tag="VG274_12665"
     CDS             4860..5285
                     /locus_tag="VG274_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07559.1"
                     /translation="MTIATTRHIDQVLAVVGGAGGSSKNGLIAKSWIRSANALGVDPG
                     SRAPPRILTLGELRASQEASSHLIEMARAELDHLYKIVQAVHYVILLCDRDGRVIGHR
                     GEEGEAAQFRHWGTWLGGCGRKKPKAPTASERASRSSDR"
     gene            5282..>5689
                     /locus_tag="VG274_12670"
     CDS             5282..>5689
                     /locus_tag="VG274_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWE07560.1"
                     /translation="MTVHRSQHFRARHISLSCSGAPIFGSDGELLGVLDVSSIDPDLS
                     EHAHALTGALTIATARAIEERLFRRQFRREWIVAVGPPDESNSAMLIAVDRDHRIVGA
                     DRNARRVLSRSGRDPLAQAASPWTIFERNDAIFR"
BASE COUNT         1005 a         1863 c         1852 g          969 t
ORIGIN      
        1 cgcggacagg tcttccacgt tcacgccgac gatcaagtac ggcaaaggct tcggtgacct
       61 gccggacgag atggccttgc tgcgacccat cgcgattacg ggaattgtcg gctatgcgat
      121 cgccggcacg aacaccaact ccatcgcatg ggccggcgcg ctcgagtaca gcctgctcta
      181 tcttcagaac aatgtccgcg accagggctt cagcaatttc gtcgcgcgtc tgacgccgat
      241 cgtcgagtac agctttactt cgcccgtcga tgcggacggc ggcgttacga ctggcacatt
      301 caatcccggt ctcatctggt cggggcagta tacgcagctc gcgatcgagg cgatcgtgcc
      361 ggccaatcgc gccagcgggg acaatgtcgg cgtgatcgcg cagctgcact tctatatcga
      421 cgatatcttt cccgatacgc tcggcaagcc gctcttcgga ggcaagccat gaacgcgcgc
      481 tcttttctcg catgccttgc catcatgggc ttcgcggcgc cggccttcgc acacgccttc
      541 ctggaacatg ccgctccggg ggcgggcgaa acgctcgcaa ctgcgccgaa gcaggttgcg
      601 ctggccttct ccgaacaact cgagccggcg ttcagcggcg tgaccgtcgc cgattccgcc
      661 ggcagcgacg tcgaatccgg ccgcgcggtc gtcgccggtg cgtcgatgag cgtcgccgtc
      721 aagccgctcg cgccgggcac gtatcgcgtc acgtggcacg cggtctcggt cgacacgcac
      781 cgtacggagg gcgcctacag cttcacggtc aagccatgat cgtgttcttc gcggcggtgc
      841 gcgcgatcca cttcgcgagc ctgatggcga tcttcggtgc gggcgccttt ctcgccttgc
      901 tgcggagccg gctgcacatc gaggtccccg cgatcgcggc gcggattccg ttcgcgagcg
      961 ccgcgaccct ggcgctgatc accgcggtcg cgtcgcttgg cctcgtcgcc ggacagatga
     1021 gcggcgactg gcacagtgcg ctcgacccca ccgcgctcac agcggtcgcc accggcacgc
     1081 gattcgggca ggtctttctc tggcgcatcg gcggacttgc ggttctctgg atcgcgtgca
     1141 tcctcaagac gccgtcgcag aatcttgcgc ccgcacttct tgccgcgctg ctacttggcg
     1201 ctctcgggct gaccagccac gccgcagcga gcagcggcga attcggcatc gttcgcgccg
     1261 gcaacgacgc ggcgcatttg ctggccggcg gattctggct gggcggcctg ctcgttctcg
     1321 ccatgctcat gtggcggcat cgcgaggcgc cggccgcgct gcttcgaccc ttgcagctct
     1381 tctccgcctg gggaacttac gcgattgccg cgctcgtcgt gacgggcgtc accaacgcgg
     1441 cctcgatcct gccggctgcg gcggtatccg tacgcagcgc ctatgcccgc gtgctgttgt
     1501 cgaaagtcgc catcgcgtcg gtgatgatcg cgctcgctgc ggtgaaccgc tggcgcctca
     1561 tgcccgcgct gcgcagtggc gagcgcggcg cgccacacga tctcgcgcgc agcatcggcg
     1621 cggagatcgc gctcggcgcc gccgtcatcg ccatggccgc ctggctcggc ctgatgccac
     1681 cgcgatgatg actcgcgcaa gcagctaaca accgacattc gaagatttat cgaactcccc
     1741 cgcgctgttc ctccccatcg tgggggaggt gccgagcccc tcggtccgcg cgaagcgcgg
     1801 ccggaggata aaccccgcga agcggagggg ggagcggcgc gcgccgcgca cggactcccc
     1861 ctccggtttg ctccgcttcg ctgcgcaaac cacctccccc acgatggggg aggaaactcc
     1921 gcagcgaggc cagatggaat gcgagccgaa actcactctg cggcgttacc tcgaaccatg
     1981 gagagggaaa acaatgaaag cgttgcgctt cgacaggttt ggtccgccgt cggttctgtc
     2041 tctccaggat gtggaggttg cgaatccccg gccgggtgaa gcgctgatcg aattgcacgc
     2101 ggcggcgatc aatccgagcg acgtgaagaa cgtcgccggc gcttttcacg ccacgctgcc
     2161 gcgcacgcca ggccgcgact atgcgggcgt ggtcttccaa ggcgacggct ggaagggcag
     2221 ggaggtatgg ggcagcggtg ccgggctggg cgtcacgcag gacggaacgc acgcccaata
     2281 tctggtggtg cccatcgact ggctctgcga aaaacccgcg cagctctcca tggagcaggc
     2341 gtcgagcgtc ggcgtcccct atctcgccgc ttggtccgcg ctcgtcgatg cggcggaggc
     2401 acaggcgggc gagaccgttc ttgtcaccgg cgcgctggga gcggttggcc gcgccgcgac
     2461 gcagatcgcg cattggaaga aagcgcgggt gatcggcggc gacaggtccg aaggcgcctc
     2521 ggacgccgac gcgttcgtga acacgagcaa gaaagatctg gccgcggagg taaaggcgct
     2581 gaccgatggc aaaggtgcgg atgtggcgct cgatacggtg ggcggcccga tgttcgagcc
     2641 tgtgctcaaa tcgcttcgcg tcggcggaag gcaggttgcg atcaccagcg cggtcaaccg
     2701 ccgggtggaa ttcgacctgg tcgatttcta tcacaatgct cttcgtctca tcggcctcga
     2761 cacgatgaag ctgaccggcc ccggcatcgc gaaaatcatg gacgcgctac gcgcgggctt
     2821 cgagagcgga gaactcaagc cgcccgagat cagagaatgg ccgctggaca aggcagtcga
     2881 agcatacgcc gcggtcgaaa agggcggttc gcaaaccaaa cacgtcctcc tgccgcaaaa
     2941 gggcttataa actgctctaa cttcgccgcc gcgggcgggc ggcgatgaag tgcgtcgtta
     3001 cgggcccttc gccggcctgg aactttctcg ccgcgtcctc ctgtgcgtga tcggcattcg
     3061 taacgcgttc gtgttgtcgc agatggtcca gccaggaatc cgacaggaag gtctcgacga
     3121 agcatccctt tttattcgga tcttcgaaca cctgccattg gtaggcgcca tcgcgtcgcc
     3181 gctcccggcc cacctgatcg agtgcttcga gaaactgttc acggttcctt gggtcgatct
     3241 cgtaggtcac catcaccatc accggtccac gatgggcgtc gaaatccgcg gtcatgaccg
     3301 gcaccggcca gtgtatcgac ggcgagagat ccagcccctt cgccatctgc agtctccagc
     3361 gccccagcag aggaatgccg gtcgcgcatc cggctgcagc tatgaaaagc gccggtccaa
     3421 ggccgaggac ggtggccgct tgcccccaga tggcgctgcc gaggctgagc gcgccaaaca
     3481 tcaccgtcac gtagatcgca agaccccgac cccgcaccca ttcgggcaac gcgtattgcg
     3541 ccgagatgtt gaggaccgaa agcatcgtca tccacgaaac gccggcgaga aggctggcgg
     3601 cgagcgcggt gacgccttca tgcgcgagtc cgtacaatgc ggtcgccgca gccgtgccca
     3661 gcgttcccgc tgcagccacc ctgtcgggca ccagccgatc gttgagccac ggcagcagga
     3721 aggcgccgcc gacggcggat gcgccgatcg ctccgagcag aatgccgtag agcatcgggc
     3781 cgccgccgat ctgatttcgc accacaaggg gcagcaacgc ccaataggcg ctcgcaaaca
     3841 gcaggaagcc ggccgctctg atcaatgtcg cgatgagcga cgcgttgtag cgtgcatagc
     3901 gaaaccccga gcggatcgcg ctcagcagat gctccggcgg aaggcgcgcg ccgctgcgca
     3961 ccggctcgtg ccaccacaca agcgcaccga ttgagccgaa attgctgacc gcattgatcc
     4021 agaacggcgc cgcgatgcca aaaacgccca gaagcgcgcc gccgagggcg ggaccgaccg
     4081 cgcggctgac attgatcccg gcgctgttcg ccaccacggc cttcggaaga tcggtcttcg
     4141 gaacgagcag ggggacgatc gcctggtagg caggcgaggt cagggcgtcg cccgcggcga
     4201 ccaggaaggc gaaaagcagc agggttatcg gcgtgacgag gtgaagccag acgagtgcgg
     4261 caaacgcggt cgcaaccgcc gcgatggaga tttccccggc aatgaggaat ttgcgcttgt
     4321 ccacgatgtc ggtcagcgca cccgccggga tcgcgaacag gaacatcggc aggctgttgg
     4381 cgacctgcac aagagagacc acgagcggcg ccgaattgag gctgagcatc aaccacccgg
     4441 aggcggccgt atacatccac cagccgacgt tcgagacgac agttgcggtc cagatcaccg
     4501 cgaacgcctt gtggcggaag ggagatcctg gcagcttcgc tcgcggagaa ctcggtggca
     4561 tttgcgtctc ggactgtgtt cgtgtcgcca ttttctaaca ccgagtatcg tggatgcgcg
     4621 cgacgttcgt cagccctcat ggttcgacaa tcgcggcatg aggacctcat ccgggtttgt
     4681 cgaaggatcg gttcggcggc ctccatcatg aggacctcat cctgagcttg tcgaaggagt
     4741 gcccaattcg tccggttgcg atggttgggt gcacggctca ccggtcacgg aagttgaacg
     4801 agaacgccgc gtaagtggta ggatggagcc gccttcggcc atctgggagg gggacgctgg
     4861 tgaccattgc gaccacccgg catatcgatc aggtcctggc ggttgtgggc ggcgcaggcg
     4921 gatcttcgaa gaacgggctc atcgcgaaat cctggataag gtccgcgaat gcgctcggcg
     4981 tcgaccccgg atcgcgcgcc cctccgcgaa ttctgacgct cggcgagctt cgcgcttcgc
     5041 aggaagcatc ttcacatctc atcgaaatgg cgcgcgccga gctcgaccat ctctacaaga
     5101 tcgttcaggc cgtccactac gtgatcctgt tgtgcgaccg ggacggccgg gtgatcgggc
     5161 atcgcggcga ggagggcgag gcggcgcagt tcagacattg gggaacctgg ctgggtgggt
     5221 gtggtcggaa gaagccgaag gcaccaacgg catcggaacg tgcctcgcgg agcagcgatc
     5281 ggtgaccgtc catcgctcgc aacatttccg agcccggcat atcagcctca gttgctcggg
     5341 tgctcccatc ttcggcagtg acggcgagct gctcggggtt ctggacgttt cgtccatcga
     5401 tccggatctg tcggaacacg ctcacgccct gacgggagcg ctcaccatcg caacggcgcg
     5461 tgcgatcgag gagcgcctgt tccgaaggca attccggcgc gaatggatcg tcgccgtcgg
     5521 acctcccgac gaatccaact ccgccatgct catcgccgtc gatcgcgacc atcgcatcgt
     5581 cggcgcggac cgcaacgcgc gccgcgtgct ctcgcgctcc ggccgcgatc ccctggcgca
     5641 ggccgcaagc ccctggacca tcttcgaacg gaacgatgca atattccgg
//
LOCUS       DASZMC010000622         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4116681,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000622 DASZMC010000000
VERSION     DASZMC010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4116681"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VG274_12675"
     CDS             <1..195
                     /locus_tag="VG274_12675"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWE07561.1"
                     /translation="LPLRLVVEIDGATHGSDEEIRHDRRRDAYLRGRGFSVLRFWNQE
                     IYENLDGVLEKIWNVVTRNA"
     gene            308..1339
                     /locus_tag="VG274_12680"
     CDS             308..1339
                     /locus_tag="VG274_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HWE07562.1"
                     /translation="MNFCSDNAYGAAPEMLEALAAANDGAAASYGEDAFTQRLQGEMS
                     RIFEREIAAFPVMTGTAANALSLATIVPPHGAVFCQRGSHIVSDECGAPEFFTHGAKL
                     VAVEGRDGKLVPKGVEDVLSQFIKGCVHHPQPAAVSIAQASELGLCYAPRETRAIAAL
                     AKRHDMKLHMDGARLANAIAHLGCTPAEATWRAGVDVLSFGATKNGALGAEAVVFFDP
                     EHVRDLEFRRKKSGHLLSKMRFVSAQLLCALEEDRWLAWAARANGFAGRLAQGLAEIG
                     SIEIVHPVQASMIFAFMPDAMVARLRAQGASFYEWAPSDGRRTLVRLVTSFMTPESDI
                     ERLLAIARQ"
     gene            complement(1397..>1999)
                     /locus_tag="VG274_12685"
     CDS             complement(1397..>1999)
                     /locus_tag="VG274_12685"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HWE07563.1"
                     /translation="FFLDTQASVGYAHLAGSRTINLEGFTRTAEDVRPAEFLAGGATA
                     GLQYNWIGAAVMPQISLDGLAMRQEGYTETHGGGQIGDGFDLHVDPDYAASLRAFTGL
                     DVRDDLNLGDFLLQPEARAGYRYDFANGQESVTANFAGVTPIDQFQISGPKPDHGNAV
                     AGGGLAISTGAWSVGLGFDYLYANSGNTSEEGTITLLGRI"
BASE COUNT          352 a          624 c          673 g          350 t
ORIGIN      
        1 ctgccgctgc ggctggtcgt cgagatcgac ggcgccacgc atggcagcga tgaagagatt
       61 cgtcatgaca ggcgccgcga tgcgtatttg cgtggccgcg gattcagcgt gctgcgattc
      121 tggaaccagg agatttatga aaatctcgat ggcgtgctgg agaagatctg gaacgtggtg
      181 acgcggaacg cgtgaaccac gcccccctcc gcctcgcttc gctcggcacc tcccccacga
      241 tgggggagga aatcagagcg cgggctcagc accggcgagg ggtagttcag atgaaggatg
      301 cttggtcatg aacttctgca gcgacaatgc gtatggcgca gcgccggaga tgctggaggc
      361 gcttgcggcg gcgaatgatg gtgcggcggc gtcctacggc gaagacgcat tcacgcagcg
      421 ccttcaaggc gaaatgtcgc gaatcttcga acgcgagatc gcagcctttc ccgtgatgac
      481 gggcacggcg gcgaatgcac tttcgcttgc caccatcgtt ccgccgcacg gcgccgtctt
      541 ctgccaacgc ggctcgcaca tcgtctccga cgagtgcgga gcgccggaat tcttcaccca
      601 cggggcgaaa ctcgtggccg tcgaaggacg ggacggcaag ctcgtgccca aaggggtcga
      661 agacgtactg tcgcaattca tcaaaggttg cgtgcatcat ccgcagcccg cggccgtcag
      721 catcgcgcaa gcgtccgagc tcggtctctg ctacgcgccg cgcgagacac gagcgattgc
      781 agcgctggcg aagcgccatg acatgaagct ccacatggac ggcgcgcggc ttgccaatgc
      841 gatcgcccat cttggctgca cgcccgccga ggcgacgtgg cgcgccggcg tcgacgtgct
      901 gtctttcggc gcgacgaaaa acggcgcgct gggggcggag gccgtggtgt tcttcgatcc
      961 cgaacacgtg cgcgaccttg aattccgccg caagaagtcc ggtcatctcc tgtcgaagat
     1021 gcgcttcgtt tccgcgcagc tcctctgcgc gctcgaagag gatcgctggc ttgcctgggc
     1081 ggcacgggcc aatgggttcg ccggccggct ggcgcaggga ctggcggaaa tcggaagcat
     1141 cgagattgtc catcccgtcc aggccagcat gatcttcgcg ttcatgcccg acgcgatggt
     1201 tgcaaggctg cgcgcgcaag gcgccagctt ctacgaatgg gcgccgtcgg acgggcggcg
     1261 cacgctggtg cgcctcgtca cctcgttcat gacgcccgag agcgacatcg agcggctact
     1321 tgccatcgcg cgccagtgac gcttttcctc ccccgctctt tgcgggggag gaaaaaggcg
     1381 gcgcgattga ggcgcgtcag atgcggccga gcagggtgat cgtcccttct tccgaggtgt
     1441 tgcccgagtt cgcgtagaga tagtcgaagc cgaggcccac cgaccatgcg ccggtggaga
     1501 tcgccagtcc gccgccggcc accgcattgc cgtgatcggg cttgggtccg ctgatctgga
     1561 actggtcgat cggcgtgacg ccggcaaaat tcgccgtgac gctctcctgg ccgttggcga
     1621 agtcatagcg atagccggcg cgggcctccg gctggagcag gaaatcgccc aaattgagat
     1681 cgtcgcgcac atccaggccg gtgaaagccc tgagcgaggc cgcgtagtcg ggatcgacat
     1741 gaagatcgaa accgtcgccg atctggccgc cgccatgcgt ctcggtatag ccttcctggc
     1801 gcatcgcaag cccgtccagg ctgatctgcg gcatgaccgc tgcgccgatc caattgtact
     1861 gaaggccggc cgtggcgccg ccggcgagga attccgcggg acgcacgtct tcggccgtgc
     1921 gggtgaatcc ttcgagattg atggtgcggc tgccggccag atgcgcgtaa ccgacgctcg
     1981 cctgcgtatc gaggaagaa
//
LOCUS       DASZMC010000623         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1089759,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000623 DASZMC010000000
VERSION     DASZMC010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089759"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            454..3075
                     /gene="ligD"
                     /locus_tag="VG274_12690"
     CDS             454..3075
                     /gene="ligD"
                     /locus_tag="VG274_12690"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HWE07564.1"
                     /translation="MPKKLTKYKAKRDFSKTAEPSGKTKVAPSQQLRFVVQKHDARRL
                     HYDFRLELDGVFKSWAVTKGPSLDPHERRLAVEVEDHPLDYGDFEGTIPRGQYGGGTV
                     MLWDRGYWAPEGTASAETQLGKGDLKFVLAGKKLQGGWVLVRMRQKERGRNNWLLIKH
                     RDGTEHDNDHDALLAKDRSVASGRTMAAIAAGKGRGPVPFMRRSRKSAKADAVWRSAG
                     APREMAKRAATPASRTKPVPAQHVSRLPQFIAPQLCTVSERAPSGADWVHEVKFDGYR
                     IQLRVGDGEAVLRTRKGLDWTGKFAAVAKLAARLPDCIIDGEVVALDDRGLPDFAALQ
                     AALSEGRSERLIYFAFDLLFEGRDDLRGLPLENRKSRLQQLLGKNSRRQIRYVEHLRG
                     AGDAVVRSACRMSLEGIVSKRLSDPYLSGRAGQWTKTKCRGGHEVVIGGWTDTNGKFR
                     SLLAGVYRGRQLVAIGRVGTGFAREKLEPLMKRLKSLASDESPFAGENAPRPRSNIHW
                     IKPELVAEIEFAGWTGDGQVRQASYKGLREDKPARDVRAERASKPAEAELAEPVPRKP
                     RTAGPGRSGESRVMNVAISHPDKPLWPAEGKERAFTKLDLARYFERVGEWLMPHVAGR
                     PCSIVRAPDGIAGQHFFQRHAMKGMSNLLDEVTVSGDRQAYLEINRIEALAAAAQLAA
                     IELHPWNCATGRPDVPGRLVFDLDPDPSVAFKAAIAAAREIKQRLEQLGLVAFCKTTG
                     GKGLHVVTPLARARGTSWPEAKAFAREVCAQMAQDSPRLYVLNMAKKERGGRIFLDYL
                     RNDRTATAVAPLSPRARPGAPVSMPLAWSSVKSGLDPKRFTIRSAPKLIAASKAWSHY
                     HEAGHSLRNAIERLAKH"
BASE COUNT          624 a          988 c         1015 g          519 t
ORIGIN      
        1 tgcggactct ccgaaatccg atgccgccct ggccaagaac cctgcagcca atgcacgcct
       61 aaatacgctc gtggccgaag cagcagccac gccctctctg gcgagcctgt ccatgcgcgt
      121 caggcacaaa accggtcacg cgctgatcgt cgctgccaat cggtaggtgg cgaggcagaa
      181 taaacagaaa aacgatttta gcgcagaggg cgcgggggaa acgcagaggc cgcagagtaa
      241 ggtttgcgtg ccggaggcgc ccgattttcc tcagcgtcct tccgtctgcg ccaaggctac
      301 ggcggatcta tagactcctt cgacccgccg tagctttagc gaaggccggc cgcgcagcct
      361 ctgcttcctc tgcgctgaat cgttcttttg gtccgacgcc aacggaaccc cgggacgttt
      421 tcgggcattg cggctttgtc gggagagccg ccgatgccga agaagctcac caaatacaag
      481 gccaagcggg atttttccaa aaccgccgaa ccgagcggca aaacgaaagt cgcgccttcg
      541 caacagctgc ggttcgtcgt ccagaaacac gatgcacgcc gcctgcatta tgacttccgg
      601 ctcgagctgg acggtgtttt caaatcctgg gcggtgacga aaggaccatc gctcgatccc
      661 cacgagcgca ggctcgcggt cgaggtcgag gaccatccgc tcgattacgg cgatttcgag
      721 gggacgattc cgcgaggtca atatggcggc gggaccgtca tgctgtggga ccgtggatat
      781 tgggcgcccg aaggcacggc gagcgccgag actcaactgg ggaagggcga tctcaaattc
      841 gtgctggcgg gaaagaagct gcagggcggc tgggtgctcg tgcgcatgcg ccagaaggag
      901 aggggccgga acaactggct tctcatcaag caccgcgacg gcacggagca cgacaacgat
      961 cacgacgcgc tgctggcaaa ggaccgctcg gttgcgtcgg gccgcacaat ggcggcgatt
     1021 gccgcgggaa aaggacgtgg acccgtgccc ttcatgcgcc ggtcgcgcaa atccgccaag
     1081 gccgacgcgg tttggcgctc ggccggggcg ccgcgcgaaa tggcgaagcg cgccgcgacg
     1141 ccggcaagca ggacgaagcc cgtgccggca cagcatgttt cgcgcctgcc ccagttcatc
     1201 gcgccccagc tctgcacggt ttccgagcgc gcgccttcgg gcgcggactg ggtgcatgag
     1261 gtcaagttcg acggctaccg gatccagttg cgcgtcgggg atggcgaagc cgtcctccgc
     1321 acgcgcaagg gcctcgactg gaccggaaaa ttcgcagccg tggccaaatt agctgcgcgg
     1381 ctccccgatt gtatcatcga cggcgaagtg gttgccctgg acgatcgcgg cctacccgat
     1441 ttcgccgcgc ttcaggccgc actctccgaa ggcagaagcg agcgcctgat ctattttgca
     1501 ttcgatctgc tgttcgaagg acgcgatgat ctgcgcggcc tcccgctcga aaaccgcaag
     1561 tcgcgcctgc agcaacttct cgggaagaat tcgcgccgcc agattcgcta cgtcgagcat
     1621 ttgcgcggcg ccggagacgc ggtcgtccgg tcggcctgcc gcatgtcgct cgagggcatc
     1681 gtctcgaaac gtctttcgga tccctacctg tcagggcgcg ccggccagtg gacgaaaaca
     1741 aaatgccgcg gcggacatga agtggtgatc ggcggctgga cagataccaa tgggaaattc
     1801 cgctcgctgc tggcaggcgt ctatcgcggc aggcagctcg tcgccatcgg cagagtgggc
     1861 acaggtttcg cgcgcgaaaa actcgaaccg ctcatgaagc gtttgaaatc gctcgcaagc
     1921 gacgaaagtc ccttcgctgg cgagaatgcg ccgcgaccgc ggtcgaacat acactggatc
     1981 aagccggaac tcgtcgccga gatcgagttc gcgggatgga cgggagacgg acaggtgcga
     2041 caggcttcgt acaagggtct gcgggaagac aaaccggcgc gtgacgtcag agccgagcgc
     2101 gcgtcaaagc ccgcggaagc agaactcgcg gaacccgtgc cgcgaaagcc gcgcacggca
     2161 ggccctgggc gatccggcga atcccgggtg atgaacgtcg ccatatcgca ccccgacaag
     2221 ccgctgtggc ctgccgaagg gaaagagcgg gcgttcacca agctcgacct cgcgcgctat
     2281 ttcgaacgcg tcggcgaatg gctcatgccg catgtcgccg gacggccctg ctccatcgtg
     2341 cgcgcgccgg acggcatcgc tggacagcac ttctttcagc gccacgcgat gaaaggaatg
     2401 tccaacctgc tcgacgaagt cacggtatcg ggcgatcgcc aagcctatct cgagatcaac
     2461 cgcatcgaag ctctcgccgc ggcggcgcag cttgcggcca tcgaattgca tccgtggaat
     2521 tgcgccacgg gccggcccga tgtgccggga cgactggtat tcgatctcga tcccgatccg
     2581 agcgtcgcgt tcaaggcggc catcgcagcg gcacgcgaga tcaagcaacg gctcgaacag
     2641 cttggcctcg tcgccttctg caagacgacc ggaggcaagg gtcttcacgt ggtgaccccg
     2701 cttgcgcgcg ccaggggaac gagctggccc gaggccaagg cgtttgcgcg cgaggtgtgt
     2761 gcgcagatgg cccaggacag ccccaggctc tatgtgctca acatggcgaa gaaggaacgc
     2821 ggcgggcgca tcttcctcga ctatctgcgc aacgaccgga cggcgaccgc ggtggcgccg
     2881 ctatcgccgc gcgcaaggcc tggcgccccg gtatccatgc cgctcgcctg gtcgtcggtg
     2941 aaatccggac tcgaccccaa acgcttcacc atccgctcgg cgccgaagct gatcgcggcg
     3001 agcaaagcgt ggagccacta tcatgaagcc gggcattctc tgcgcaatgc aatcgaacgg
     3061 ttagcaaaac actaagacac gcgcgtattt attctggtat ccgcgggatg cgagttgttg
     3121 tctgcgggca atttttaaca gtttgt
//
LOCUS       DASZMC010000624         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3812073,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000624 DASZMC010000000
VERSION     DASZMC010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3812073"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..439
                     /locus_tag="VG274_12695"
     CDS             161..439
                     /locus_tag="VG274_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07565.1"
                     /translation="MSQSETPKPKYPPPTGGGAGAAALFVIGVLILLPSGLCSAVFGV
                     GAIFELFNNPGALANDFLDILPMIIVTVVFLIIGILMVRASLHAKKKP"
     gene            482..1021
                     /locus_tag="VG274_12700"
     CDS             482..1021
                     /locus_tag="VG274_12700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWE07566.1"
                     /translation="MEPLALSIRLARPADAAADARVYIDSWHDTYPGVLSKALLCSMT
                     PKGQAARWLASINRKELVLVAEHDKHGIVAMASAGAVRDRGLGFDGEVFTLYVDPGHF
                     GCGAGRQLLTGTFTELRKRGFRSCVIWAHAKNNARFFYEAMGGKLVAERTARMMGDAV
                     PEAAFGWKTLALTERSVAR"
     gene            complement(1022..1546)
                     /locus_tag="VG274_12705"
     CDS             complement(1022..1546)
                     /locus_tag="VG274_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWE07567.1"
                     /translation="MKRLLLLRHGKAEGAASDDRKRALIPRGKTDSARMGRFLHEDAY
                     IPDLILCSDSTRTRQTVDAVLPELQVWPEVKYLAELYLAEPELVLSLVRRANDNLESL
                     MVVGHNPGLEQCALALAAVPLEKKFRKRYDVMDEKFPTCALAVIDFDVGKWRDVTPGR
                     GDLDAFVRPKDFES"
     gene            complement(1543..1959)
                     /locus_tag="VG274_12710"
     CDS             complement(1543..1959)
                     /locus_tag="VG274_12710"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HWE07568.1"
                     /translation="MSVNTISHQTALVYVMIVVAAADGVISDKELKTIGELSKHLPAF
                     RDFDHSHLLSVAQDCGAILQEPEGLDAVMGLAKAALPDHLRETAYWLALEVALSDSRV
                     MLEEIRVIEAIRRSLGIDKLTAAALERGARARYQVA"
     gene            2035..3228
                     /gene="metZ"
                     /locus_tag="VG274_12715"
     CDS             2035..3228
                     /gene="metZ"
                     /locus_tag="VG274_12715"
                     /inference="COORDINATES: protein motif:HMM:NF006003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HWE07569.1"
                     /translation="MATNKKPGKWRARTMAVRGGQTRSDFQETSEALFLTSGYAYESP
                     TQAEGRFTGEDDGFQYTRFSNPTIQMFEDRMAALEGAPVARATASGMAAVSAALLSGL
                     SAGDHIVSAKAMFGSCRTVIIDILPRFGVTHTLVDGSDLDQWRTAMRPNTKMLFLETP
                     ANPTLDIVDIAAVAKIADAGGARLVVDNAFASPVLQQPMALGAHIVVHSTTKWVDGQG
                     RCLGGMILCREDFLKDHLQLYLRNTGPVMSPFNAWVHLKSLETLDLRMRAHCENAMMV
                     ANFLGGQKKVTRVLYPFHPDHPQHNLARAQMSGGGGVVTFEIAGGKNGAFRFAEALKL
                     IDISNNLGDAKSLITHPETTTHQRLSPEGRAELGITSGQMRLSVGLEDADDIREDLEQ
                     ALEAA"
BASE COUNT          639 a         1012 c         1002 g          648 t
ORIGIN      
        1 atcgcgatcg tgtcatttct cgatcgctac aacttctgca tcgcggcggt gaagcgctcc
       61 tgcattcatt tcgtgacgcc gggcggcaag atcatcccat tcgacactta caatcttttc
      121 tatcgcagtg gattggacgc aaaactgagg gagactgtcc atgtcccagt ctgaaactcc
      181 gaaacctaaa tacccaccgc caaccggtgg cggcgcgggt gccgcggcac tgttcgtcat
      241 cggcgttctg atcctgctgc cgtccggcct ttgttcagcg gtcttcggcg ttggagcaat
      301 ttttgaactc ttcaacaatc cgggggccct cgcgaacgat tttctcgaca tacttccgat
      361 gatcatcgtc acggttgttt ttctcattat cggaattctg atggtgcggg cgtcgttgca
      421 cgcaaaaaag aagccctgat cggcttgccg atctcatcca gttgcgcgaa gcttcgtgtc
      481 gatggaaccg cttgcgctct cgatcagatt ggcgcggccc gcagacgccg ccgctgacgc
      541 gcgcgtctat atcgactcct ggcacgatac ctatccgggc gttctgtcca aggcactgct
      601 atgctccatg acgcccaaag gtcaggcggc acgctggctc gcgtcgatca accggaagga
      661 actcgtgctg gtggcggagc atgacaagca tggcatcgtc gcgatggcga gcgccggcgc
      721 tgtgcgcgac cgcggacttg gtttcgacgg cgaggtgttc acgctctatg tcgatcccgg
      781 ccatttcggc tgcggcgcgg ggcggcaatt gctgacgggc acgttcacgg aactgcgcaa
      841 gcgcggcttt cgctcctgcg tgatctgggc gcacgcgaag aacaacgcac gtttcttcta
      901 tgaagccatg ggcggaaaac tcgtcgccga gcgcaccgcc cgcatgatgg gcgacgcagt
      961 tccagaagcg gccttcggct ggaaaacgct ggctctcacc gaacgcagcg tggcgcgcta
     1021 gtcagctctc aaaatctttc gggcgcacga acgcatcaag gtcaccgcgg ccgggcgtga
     1081 catcgcgcca tttcccaacg tcgaaatcga tcacggcgag cgcgcaggtc ggaaatttct
     1141 cgtccatgac gtcgtagcgt ttgcggaatt tcttctctag cgggacagcg gcgagagcga
     1201 gcgcgcattg ctccagtccc ggattgtggc cgacaaccat cagcgattcc aggttgtcgt
     1261 tcgcgcggcg aacgagcgag aggaccagtt ccggctcggc gagataaagc tccgccagat
     1321 atttgacctc gggccagact tgcagctccg gcagaacggc atccactgtt tggcgcgtgc
     1381 gagtcgaatc cgaacacaag atcaagtcgg gaatatacgc gtcttcgtgc aggaagcgcc
     1441 ccatgcgcgc gctgtcggtt ttgccgcgcg gaatgagcgc gcgctttcga tcgtcggagg
     1501 ccgcgccctc cgccttgccg tgacgaagga gcagcagccg tttcacgcga cctgataccg
     1561 cgcgcgtgcg ccgcgctcga gcgccgctgc tgtgagtttg tctatgccaa gcgaacggcg
     1621 gatggcctcg atcacccgga tctcctccag catcacgcgg ctgtcggaga gcgccacttc
     1681 aagggccagc caataggccg tctcacgcag atgatcgggc aacgcggcct tggccaggcc
     1741 catcacggcg tcgagtcctt ccggctcctg caggatcgcg ccgcaatcct gcgcgacgct
     1801 cagaagatgg ctgtgatcga aatcgcggaa cgccggcagg tgtttcgaca gctcgccgat
     1861 cgtcttcagc tccttgtcgc tgatgacccc gtcggccgcc gccacgacga tcatgacgta
     1921 gacgagcgcc gtttggtggc tgatggtatt cactgacatg tcggaccgcg atttggaatg
     1981 cggcgcgacc ttaagaaact tgacccccag cggcaaggca cgtcacaaac acctatggcg
     2041 accaacaaaa agcccggcaa atggcgcgcg cgaacgatgg ccgttcgcgg cggccagacg
     2101 cgctcggatt ttcaggaaac ctccgaagcg ctgttcctga cctctggcta tgcctatgag
     2161 tcgccgacgc aggcggaagg ccgctttaca ggcgaggacg acggctttca atacacgcgc
     2221 ttctccaacc ccacgatcca gatgttcgag gatcgcatgg cggcactgga gggcgcgccc
     2281 gtcgcgcgcg cgacggcgag tggcatggcg gcagtgagtg cggcgttgct gagcggtctc
     2341 agtgccggcg atcacatcgt gtcggcgaag gcgatgttcg gatcatgccg cacggtgatc
     2401 atcgatatcc tgccccgttt cggcgtgacc catacgctgg ttgatggcag cgatctcgat
     2461 cagtggcgca cggcgatgcg cccgaacacg aagatgctgt tcctggaaac gccggccaat
     2521 ccgacactcg atatcgtcga tatcgcggcg gtggcaaaga tcgccgatgc cggcggtgca
     2581 cggctagtcg tcgacaacgc attcgcgagc ccggtacttc agcagccgat ggcgcttggc
     2641 gcacacatcg tcgtgcattc gacaaccaag tgggtcgacg gccagggccg ctgtctcggc
     2701 ggcatgatcc tgtgccgtga ggattttctg aaagatcacc tgcagcttta tctgcgcaac
     2761 accggaccgg tgatgagccc attcaacgcg tgggtgcatc tgaaaagtct cgagacattg
     2821 gatctgcgca tgcgcgccca ttgtgagaat gcgatgatgg ttgcgaattt tctcggcggg
     2881 cagaagaaag tgacgcgcgt tctttatcct tttcatcccg atcatccgca gcacaatctc
     2941 gcccgcgcac agatgtccgg cggaggcggt gtcgtgacgt tcgaaattgc cggcggaaag
     3001 aacggcgcgt tccgttttgc cgaggcgctg aagttgatcg acatttccaa caacctgggc
     3061 gacgcgaaaa gcctcatcac ccatcctgaa acgacaacgc accaacgtct atcgcccgag
     3121 ggccgtgccg agctgggcat cacgtccggc cagatgcgtc tctccgtagg cctcgaagac
     3181 gcggacgaca tccgcgaaga tctggaacag gcgctggagg ccgcatgacc tcgttgaaaa
     3241 tggcgcgcgt gatcctgttc accggccaga tcgacgccat gagcgaattc tatggcaagg
     3301 c
//
LOCUS       DASZMC010000625         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1091097,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000625 DASZMC010000000
VERSION     DASZMC010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091097"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VG274_12720"
     CDS             <1..681
                     /locus_tag="VG274_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07570.1"
                     /translation="RPTGGIATIKRWAGGGCATTEPSHQIRLADVQSKRMLMGFIGFG
                     STPPAPPPAEEPPGDEGGDDTGIRVTPAARSDRSLYRRRGPAAGRYAWVISLGIHAAV
                     LVGAFLAMKYYFRQPALPKPASVSEDSSATGSIIQSADSTDLVHGSMSITINPDAVGE
                     QPFMDGKELPAFVLGQPRTIATLSDLAPGSGIDALPQGAITALGGSPVRSSHRASPAT
                     QPSRVTLP"
     gene            complement(668..1558)
                     /locus_tag="VG274_12725"
     CDS             complement(668..1558)
                     /locus_tag="VG274_12725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWE07571.1"
                     /translation="MPLPKISIVTPSYNQAPFIAWTVRSVLLQRYPNLEYILMDGGSN
                     DGTQEVLEPYRERFAHYQSARDKGQSEAIHNGFNHSTGDIMAYLNSDDMLAPGTLHFV
                     AEYFQSHPEVDAVYSHRCTVDENNKAVYYWILPRHSNWYMSRWDLIPQETCFWRRSLF
                     ERAGNIDPEYRFAMDYDLFVRFMLKGKMTRANRFLGVFRTHTAAKTSQLLETIGSEEI
                     ARVWKKYGLVKRKIDSLLAARFFNSVNRAGGKFAHRRRHLPGALPGIGYDYDRVWGGL
                     LNDKRLPPRASAMLPSFTAG"
BASE COUNT          299 a          537 c          548 g          332 t
ORIGIN      
        1 cgccccaccg gggggatcgc cacaatcaag cgctgggccg ggggcggctg tgctacaacc
       61 gagccgtcgc accaaatccg cttggctgac gtacaatcaa aacgcatgct gatgggattc
      121 attggctttg gcagcacgcc accggcgcca cccccggcgg aggaaccgcc gggcgacgag
      181 gggggcgatg acaccggtat ccgcgtcacg cccgcggcgc ggtccgatcg ctctctctac
      241 cgtcggcgcg ggccggcggc ggggcggtat gcgtgggtca tttccctggg gattcacgcg
      301 gcggtgctgg tgggcgcgtt cctggcgatg aagtattact tccgccagcc ggcgctgccg
      361 aaaccggctt ccgtttccga agattcctcc gccaccgggt cgatcattca gagcgcggat
      421 tcgactgacc tcgtccacgg cagtatgagc atcacgatca atcctgacgc cgttggcgaa
      481 cagccgttca tggatggcaa agagcttccc gcgtttgttc tcggccagcc gcggacgatt
      541 gcgacgctca gcgatcttgc ccccggcagc ggcattgatg cgttaccgca aggagcgatc
      601 accgcgctcg gcggttcgcc ggttcgctcg tcgcaccgcg cttctcccgc gacgcagccg
      661 agccgtgtca ccctgccgtg aatgagggga gcatcgcgct cgccctcggc ggcaatcgct
      721 tgtcgttcag caaccctccc cacacgcgat cgtagtcgta tccgatgccc ggcagcgcgc
      781 cgggaaggtg ccgccggcga tgggcgaatt tcccgccggc gcgattgacg ctgttaaaga
      841 accgcgcggc gaggaggctg tcgatctttc gtttcaccag cccatatttc ttccacacgc
      901 gggcgatctc ctcgctgccg atcgtctcca gcaattgcga tgttttggcg gcggtgtgtg
      961 tgcggaacac gccgaggaag cggttcgccc gcgtcatctt tcccttcagc atgaagcgca
     1021 cgaacaggtc gtagtccatt gcgaagcgat actcgggatc gatgtttccc gcgcgctcga
     1081 agaggctccg ccgccagaag caggtctcct gcgggattaa gtcccagcgg ctcatgtacc
     1141 agttgctgtg ccgcggaaga atccagtaat agacggcctt gttgttctca tcgacggtgc
     1201 atcggtggct gtagaccgca tccacctccg gatggctttg gaagtattcg gcgacaaagt
     1261 gcagcgtgcc gggggcgagc atgtcgtcgc tattcagata ggccatgatg tcgccggtcg
     1321 agtgattgaa cccattgtgg atggcctcgg actggccttt gtccctggcg ctttggtaat
     1381 gggcaaagcg ctcgcgatag ggctcgagca cttcctgcgt gccgtcattc gagccgccat
     1441 ccatcaggat gtattcgaga ttgggatagc gctgcagcag gacgctgcgc accgtccatg
     1501 caatgaacgg cgcctggttg tagctcggcg tgacgatgga aatctttggc agcggcattg
     1561 attcgataga caaaaagtta gaccggtcgg aggacggcaa tcatgtaatc gccccggccg
     1621 cgccagtccc atagcagccg gcaccagccg cacaggcgcg tgccgtgcat ggcgatcaca
     1681 ttcgggaaga gtgcgctgtg cggcacgacc gcgccg
//
LOCUS       DASZMC010000626         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4656299,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000626 DASZMC010000000
VERSION     DASZMC010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4656299"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VG274_12730"
     CDS             <1..799
                     /locus_tag="VG274_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWE07572.1"
                     /translation="GAVGKIVAVNGNSRFAEGDYVSNFSGWREWFVSSGGDLQKIDPA
                     MAPIQAYLGTFGMPGLTAYAGLLKIAELKDGERVFVSAASGAVGSVVCQIAKNKGCMV
                     VGSAGSDEKCDWLVHEARVDHAINYRSCGALDAAIRKAMPDGIDVYFENVGGEHLNAA
                     LANMRLSGRIAACGMIAQYNDTSLPPGPPNIVMVIPLRLTVRGFIVSDHFDMMPDFIR
                     DMAAWAKAGKMKWRETIVDGIEKAPEAFIGLFKGENFGKMLVRLAKE"
     gene            complement(963..1604)
                     /locus_tag="VG274_12735"
     CDS             complement(963..1604)
                     /locus_tag="VG274_12735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWE07573.1"
                     /translation="MIAMVVFDVGETLVDETRHWSDWADDIRVPRFTFLAALGGVIAR
                     GQHHRDVFRLLIPDFDYEAAHRARLARGWRYSFEPHDFYPDAIACLRELKARGFRVGI
                     VGNQHRECEAEFNKLGLVLDLIGSSERWGVEKPLSGFFARIAQESGLQPAQIAYVGDH
                     PVNDVAGAARAGMVPIFLRRGPWATLHADSPEAAQAKIRLENLAELPDRLAVL"
     gene            complement(1683..2432)
                     /locus_tag="VG274_12740"
     CDS             complement(1683..2432)
                     /locus_tag="VG274_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07574.1"
                     /translation="MGAARLLAKGWVLFCVYGGGLALDRALAGGAPAAEAAWAIGVCV
                     FLFGAMGLLFISGYGLSAGHVRLFGPTGLKPSQIIPGFNELVFLGFALVMFCVQNFYA
                     PTQHSGPVVDAIESAIRFAVFGQRAFADALLLCGKDGGRLFVAAFSWMLALIFLASAL
                     SRVRLAAAIVCIERKRHPEALGAQPLAFALGLAAVAGVQLLYVGTGYRLLSCHGLAGI
                     WGDTVIGVGPLAFAYVVVAALTNLLALGPEA"
     gene            complement(2464..>3917)
                     /locus_tag="VG274_12745"
     CDS             complement(2464..>3917)
                     /locus_tag="VG274_12745"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HWE07575.1"
                     /translation="LRRNGSRNDAVPTYTLVQSRKPANVTQIWSPASWRGKPIRQVPV
                     YPDPGALEAVETRLKTHPPLVFAGEARSLKARLAQVVEGKAFLLQGGDCAESFAEFHP
                     DNIRDTFRVLLQMAVILTFAAAMPVVKLGRIAGQFAKPRTADMETRGALTLPSYRGDN
                     INAMEFDAASRTPDPARLMQAYAQSASTLNLLRAFAQGGYADLHNVHRWTLGFIAGSP
                     SGERYRALAQRISETLEFMEACGITPDSTPQLRVTEFYTSHEALHLGYEQALTRIDST
                     SGDWYDTSAHFLWIGDRTREASGAHVEFCRGIRNPVGLKCGPSLDECELLRLLSILNP
                     GNDPGRVTLICRFGADKAVEKLSRLIRAVVREGAAVVWSCDPMHGNTITSHMGLKTRS
                     FERILKEVKTFFDVHRAEGTHAGGVHVEMTGQNVTECLGGAQAISEEDLSRRYNTHCD
                     PRLNANQALELAFLMAESLRAERLKDARFAASA"
BASE COUNT          797 a         1235 c         1222 g          663 t
ORIGIN      
        1 cggcgccgtg ggaaaaatcg ttgccgtaaa cggcaattcc cggtttgcgg agggtgacta
       61 cgtctccaat ttttccggct ggcgcgagtg gtttgtgtcg agcggcggcg atctgcagaa
      121 aatcgatccg gccatggcgc ccatccaggc gtatctgggg acgttcggca tgcccggcct
      181 caccgcctat gccggccttc tcaaaatcgc agaattgaag gatggcgaac gcgtgttcgt
      241 ctcggctgcg agcggcgcgg tggggtccgt cgtctgccag atcgcgaaga acaagggatg
      301 catggtcgtg ggctccgccg gctccgacga gaaatgcgac tggctcgtgc acgaggcgcg
      361 cgtcgatcat gcgatcaact atcgaagctg cggtgctctc gacgcagcga tccgcaaggc
      421 gatgccggat ggcatcgacg tgtatttcga gaatgtcggg ggcgagcacc tgaacgcggc
      481 gctcgccaat atgcgcctct ccggccgcat cgccgcgtgc ggaatgatcg cgcagtacaa
      541 cgacacgtcg ctgccgccgg gtccgccgaa catcgtcatg gtgattccgc tgcggctgac
      601 ggtccgggga ttcatcgtca gcgatcattt cgacatgatg cccgatttca tccgcgacat
      661 ggcggcctgg gcgaaggcgg gaaagatgaa atggcgcgag acgattgtcg acggcatcga
      721 aaaagctccc gaagctttca tcggtctctt caagggcgag aatttcggca agatgctggt
      781 gcgtctggcg aaggagtaac ggaccgggaa gttcatatgg aatccggcga ccggaaactc
      841 gagtgcaccc cgaaaaagaa gagacacgat caatgagaaa cggttctcaa gagaagaact
      901 gaagtctgct ctgcagttat catatgcgtc gggcaacgcc caaggaagtt agcagtgtag
      961 cctcagagta cggccaggcg atccggcagc tccgcaagat tttcgaggcg aatctttgcc
     1021 tgcgccgctt ccggcgaatc cgcgtggaga gtggcccatg gcccgcgccg caaaaaaatt
     1081 ggcaccatgc ctgcacgcgc cgcgccagca acgtcgttca ccggatgatc cccgacatag
     1141 gctatttgcg cgggctgcag tccgctctct tgcgcgatgc gcgcgaagaa tccggacaag
     1201 ggtttttcaa cgccccaccg ttccgaagag ccgatgagat cgaggaccaa gccgagcttg
     1261 ttgaattcgg cctcacactc gcgatgctga ttgccgacga tgcccaccct gaagcctcgc
     1321 gctttcagct cccgcaggca cgcgatggcg tctggataga aatcatgtgg ctcgaaacta
     1381 tagcgccagc cgcgcgcaag gcgcgctcta tgcgcggcct cataatcgaa atcgggaatg
     1441 agcaatctga agacatcgcg atgatgttgt ccccgcgcaa tgacaccccc gagcgcggcg
     1501 agaaatgtga agcggggcac ccgaatgtca tccgcccaat cggaccagtg ccgcgtttca
     1561 tccaccagag tttcgccgac atcgaagaca accattgcta tcatcagagc aaccccagcg
     1621 ccgtcccttc cgcccacagc cgcaaccagc gcacgcttgc cgccattcag tctgtggata
     1681 ttctacgcct cgggcccgag cgcgagcagg ttcgtcagcg cggccactac cacataggcg
     1741 aacgccagag gaccgacacc gatcaccgtg tcgccccaga ttcccgcgag cccatggcac
     1801 gacagaagcc tgtagccggt gccgacatag agaagctgga cgccggcaac agccgcgagt
     1861 ccaagtgcaa acgccagcgg ttgcgcgccg agcgcttcgg gatggcgctt gcgttcgata
     1921 cacacgatgg cggcggcgag ccggacgcgc gacagcgcgg aggcgagaaa tatgagcgcc
     1981 agcatccagg aaaacgccgc aacgaacagc cgccctccat ccttgccgca gagcaagagc
     2041 gcgtcggcga atgcgcgctg tccgaagacg gcgaagcgga tcgcgctctc gattgcgtca
     2101 acaacgggac ccgagtgctg cgtgggcgcg tagaagttct gcacgcagaa catgacaagg
     2161 gcgaacccca gaaacaccag ctcgttgaat cccgggatga tctgggaggg cttcagtccg
     2221 gtggggccga acaggcggac atggcccgcc gacagcccat atccggagat gaacaaaagt
     2281 cccatcgctc cgaacagaaa cacacagacg ccgatcgccc acgccgcttc cgcggcaggc
     2341 gcaccgccgg ccagtgcccg gtcgagcgcc aagcctccgc cgtagacgca aaacagaacc
     2401 cagcccttcg caagcaggcg cgccgcgccc atgaatctcc ccctcctccc ctgaccgcgg
     2461 gcctcaggcc gaggccgcga agcgcgcatc cttcaggcgc tcggcgcgca aactctccgc
     2521 catcaggaaa gcaagctcaa gcgcctggtt ggcattgagc cgcggatcgc aatgcgtgtt
     2581 gtagcggcgt gacaaatcct cttccgaaat cgcctgcgcg ccgcccaggc attcggtgac
     2641 gttctggccg gtcatctcga catgcacgcc gccggcgtgc gtgccttctg cacgatgcac
     2701 gtcaaaaaac gtctttacct ccttgaggat gcgctcgaag gagcgggtct tgaggcccat
     2761 atgcgatgtg atcgtgttgc cgtgcatggg atcgcaggac cacacgaccg cggcgccctc
     2821 ccgcaccacg gcgcggatca ggcgcgacag tttctctacc gccttgtcgg cgccgaagcg
     2881 gcaaatcagc gtcacccgtc cagggtcgtt cccgggattg aggatggaga gaagcctgag
     2941 caactcgcac tcatcgagtg acggaccgca tttcaatccg acaggattcc tgatgccacg
     3001 gcagaactcg acatgggcgc ccgatgcctc gcgcgtgcga tcgccgatcc acaggaaatg
     3061 cgctgaagtg tcgtaccagt cgccgcttgt cgaatcgatg cgcgtgagcg cctgttcgta
     3121 gccgagatgc agcgcttcgt ggctggtgta gaactcggtc acgcgcagtt gcggcgtcga
     3181 atccggcgtg atgccgcagg cctccatgaa ttcgagagtc tcggaaatgc gttgtgcgag
     3241 cgcccggtag cgctcgcccg acggagaacc cgcgatgaat cccagcgtcc agcgatgcac
     3301 attgtgcaga tcggcataac cgccctgggc gaaggcgcgc aaaagattga gcgtcgaagc
     3361 ggactgcgca taggcctgca tcagccgcgc gggatcgggg gtgcggctcg ccgcgtcgaa
     3421 ttccatcgca ttgatgttgt cgccgcgata ggacggcagc gtcagcgcgc ctcgcgtctc
     3481 catatcggcg gtgcgcggct tggcgaactg tcccgcgatg cggccgagtt tcaccaccgg
     3541 catggcagcg gcgaaggtca ggatcaccgc catctgcagg agcacgcgga acgtgtcgcg
     3601 gatattgtcg gggtggaact cggcgaagct ctcggcgcaa tccccgccct gcagaaggaa
     3661 agctttcccc tcgacgacct gggcgagccg cgccttcagg ctgcgcgcct cgccggcgaa
     3721 tacgagcggg ggatgggtct tcagccgcgt ctcgacggct tccagagccc ccggatcggg
     3781 ataaacggga acctggcgga tcggcttgcc gcgccagctt gctgggctcc agatttgtgt
     3841 cacgttcgca ggcttccttg attgcacgag ggtatacgtc ggaacggcat cgttccgcga
     3901 gccgtttctc ctcaggc
//
LOCUS       DASZMC010000627         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5205942,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000627 DASZMC010000000
VERSION     DASZMC010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5205942"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VG274_12750"
     CDS             <1..906
                     /locus_tag="VG274_12750"
                     /inference="COORDINATES: protein motif:HMM:NF036590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HWE07576.1"
                     /translation="LLTANAKALHNGDEPRNDDGLMAALGAMLANGDKHEPAFIALTL
                     APPSEADVAREIGSNVDPNAVFAARRTLRAAIGKTHGAALTKTCERMVSTEPYSPDAP
                     SAGRRSLKNVCLDLLATTGAPEALARARKQYASADNMTDRMAALETLAMHDRNGRGDA
                     LDDFYKRYANDPLIIDKWMALQAAIPEPETLDRVKSLATHQAFSMANPNRVRSLIGAF
                     AQANHTQFNRADGAGYDFVADIVLELDPKNPQVAARIIGAFRSWRALEPTRRARAHQT
                     LRRVSQAPSLSRDVHDIVARTLAEG"
     gene            1166..>2822
                     /locus_tag="VG274_12755"
     CDS             1166..>2822
                     /locus_tag="VG274_12755"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HWE07577.1"
                     /translation="MARAEAASASLQPDSIKGLAASIANPAYRRLLTAEPLLRRAVPV
                     LIVAFMLTICVGAAVQVLEHRRQAIVDSVRTGEALADHLATALDRISRDGKPLLPRTP
                     EALSHALPAWAEGAGRSILIADADGNIVASAPNEPTWQGRHILDVLGPMQPLPTTTSA
                     AIETTENTMPDGDSVFATVRALRNPIGFLAVVQPRSDALASWRSTTALTVTLSATTGF
                     VVLILGFAFHWQATRAREADLIHDTVRSRIDTALNRGHCGLWDWDLARGRVFWSHSMF
                     DLLGLEPKDDLLNFGEVNALVHQDDIDLYELANKVADARVQSIDHEFRMRHADGRWLW
                     LRARCETHQDSDGGLHLIGIAVDVTEQKSLVERTVAADLRLRDAIETIPEAFVLWDAS
                     NRLMLCNSNFQQLHHLPDGAVQPGARYETVVAAGKKPILRTTVTNDGGLRPGARTFEA
                     ELDDGRWMHISERRTKDGGYVSVGTDITKIKLHEQKLVEGEKRQKATIDDLRKSEEAL
                     TLHTEQLADLAQKYAEEKNRAEDANQAKSKFLANMSHELRTPLN"
BASE COUNT          594 a          916 c          810 g          502 t
ORIGIN      
        1 ctcctgaccg ccaatgcaaa ggcactgcat aatggcgacg agccgcggaa cgacgacgga
       61 ttgatggcag cgctgggcgc catgctggcg aacggcgaca agcacgagcc cgcctttatc
      121 gcgttgaccc ttgcgccacc gagtgaagcc gacgttgccc gcgagatcgg cagcaacgtc
      181 gatcccaacg cggtgttcgc tgcgcggcgc accttgcgcg ccgccatcgg caagactcat
      241 ggagcagcgc tcacgaaaac ctgtgaacgc atggtttcga ccgaacccta tagcccggat
      301 gcgccaagcg ccggccgccg atctctcaag aacgtctgcc tcgatcttct tgccacaaca
      361 ggcgcgccag aagcacttgc gcgagcgcga aaacaatacg cttcagccga caatatgacc
      421 gatcgcatgg cggcgctgga gacgctggcg atgcacgatc gtaacggccg cggcgatgcg
      481 ctggacgact tttataaacg ctacgccaac gatccgctga tcatcgacaa gtggatggcg
      541 cttcaggcgg caatcccgga accggaaacg ctcgatcggg tcaagtcgct ggccacccat
      601 caggcatttt ccatggccaa tcccaaccgc gtccgctccc tcatcggcgc atttgcgcag
      661 gccaaccaca cgcagttcaa ccgcgccgat ggagcaggct acgatttcgt cgccgatatt
      721 gtgctggagc tggaccccaa gaacccgcag gtggccgctc gtatcatcgg cgctttccgc
      781 tcctggcgag cgctggaacc gacccggcgc gcccgcgcgc atcaaacctt gcgccgcgtt
      841 tcacaagcac cttcgctctc gcgtgacgtc cacgatatcg ttgcccggac gctggccgag
      901 ggctgaccat ccgctttgaa atgccgcgct ggcgtgtcga ccgcgtaagc gattcaacgc
      961 aaaagaaaat ttgagatccc tcccgccttc ttttttatcg ccgctgataa agctttggat
     1021 cgttaaggaa aaatattgac tcgtcgagtt cccaaacact cgacaaatcg attcgcatcg
     1081 attcttatca gaagatgatt cggacgggac acggcaaggt ctttccgaat ccggtctagg
     1141 ggatttcgtt caaacggagg caggcatggc gcgcgccgaa gcggcgagtg cgtctttaca
     1201 gccggattcg atcaagggct tggcagcctc gatcgccaac cccgcgtatc gacgcctctt
     1261 gaccgccgag ccgctgctgc gccgcgcggt gcccgttctc atcgttgcat tcatgctgac
     1321 catttgcgtc ggcgccgctg tccaggtgct cgaacatcgc cgccaggcca ttgtcgattc
     1381 agtacgcacc ggcgaggcgc ttgccgatca tcttgccacc gctcttgatc gcatttcgcg
     1441 cgacggcaaa ccgttacttc cccgtacgcc agaggcgctg tcccatgccc tccccgcatg
     1501 ggctgagggc gcgggccgca gcattttgat cgccgatgcc gacggcaaca ttgtcgccag
     1561 cgcgccgaac gaacccacat ggcaaggacg ccatatcctt gatgtcctcg gaccgatgca
     1621 gccgttgccg acaacaacat cggcggcgat cgagaccacc gaaaatacca tgcccgatgg
     1681 cgactcggtg tttgcgaccg tgcgtgcact ccgtaatccg attggttttc ttgccgtggt
     1741 gcaaccgcga agcgacgcac ttgcgagctg gcgctcgacc accgcactca ccgtcacact
     1801 ctcagccacg accggtttcg tggtgctgat cctgggtttt gccttccatt ggcaagccac
     1861 acgcgcccgc gaggccgacc ttattcacga tacggtacgc agccgcatcg acaccgccct
     1921 caaccgcggc cactgcggcc tgtgggactg ggacctcgca cgtggccgcg tgttctggtc
     1981 acactcgatg ttcgacttgt tgggccttga accgaaagac gaccttctca atttcggcga
     2041 agtcaacgca ctcgtgcatc aagacgacat cgatctctat gagctggcga acaaggtcgc
     2101 cgatgcgagg gtgcaatcga tcgatcacga gttccgtatg cgccacgccg atggccgatg
     2161 gctctggctc cgcgcccgct gcgagaccca tcaagatagt gatggcggcc tccacctgat
     2221 tggcattgcc gtcgacgtca ccgagcaaaa gagcctggtc gagcggacgg tggcagcgga
     2281 cttgcgcctg cgtgacgcca tcgaaacaat accggaagcc ttcgtgctgt gggacgccag
     2341 caaccgcctg atgctgtgca actcaaactt tcagcagttg catcatctgc cggacggagc
     2401 ggtccagccc ggggcgcggt acgagaccgt cgtcgccgca ggcaagaagc cgatcctgcg
     2461 aacaacagta acgaatgacg gtggactgcg ccccggcgcg cgcactttcg aagcggaact
     2521 cgacgacggg cgctggatgc acatcagcga gcggcgcacc aaggacggcg gctatgtttc
     2581 ggtcggcacc gatatcacga agatcaagct gcacgagcag aagctggtcg agggcgagaa
     2641 gcggcagaaa gccacaatcg acgatctgcg caaatccgag gaagcgctga cactgcacac
     2701 agagcagctc gccgacttgg cgcagaaata cgcggaggaa aagaaccgcg ccgaggacgc
     2761 caatcaagcc aaatcgaaat tcctggccaa tatgagccac gaactgcgca cgccacttaa
     2821 cg
//
LOCUS       DASZMC010000628         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_259592,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000628 DASZMC010000000
VERSION     DASZMC010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259592"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VG274_12760"
     CDS             complement(<1..693)
                     /locus_tag="VG274_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07578.1"
                     /translation="MLAYIARRLLLMIPTIFGVMLVSFAIVQFVPGGPVERAIAQLQG
                     TDQGSTANFGGAGGGGIGAGATGQAGGDISSRYRGAQGLDPKFIKELEKQYGFDKPAW
                     QRFWILVRDYSTFHLGDSYYRNAPVVQLIKEKLPVSMSLGLWMTVISYAISVPLGIRK
                     AVTDGSRFDIWTSAAVIFAYAIPSFLFAILLVVLFCGGSFWQLFPLRGLTSDNFADFS
                     WPHKILDYLWHIT"
     gene            complement(812..2677)
                     /locus_tag="VG274_12765"
     CDS             complement(812..2677)
                     /locus_tag="VG274_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWE07579.1"
                     /translation="MGIIISRRAALKGTAASLAASLSVGWPLARAEDEIETHGLSAFG
                     DLALPPDFMSLAYVNPNAPKGGLLALLVSTTTGNQNFETFDTLNVFSKKGDGAAGMLA
                     TYDSLMGGNEDEPDSVYGLIARSVRYSQDKLTYRFLLRPEARFSDGSPLRAADVVFSL
                     TTLKEKAHPYFSRLLTEVESVEAEAEDVVKIRFIKNRTRDAHLIVAGMPVFSAAWWKG
                     RDFDAATLEAPLGSGAYRVKAFEQSRFIEFELDHAYWAKDHPLNVGGANFQRLRYEYF
                     RDRQVAFEAFKAGVVNFNEEHTSRFWANNYEFAAVKEGRVVKETLHNGAPTGSQGWYF
                     NTRREQFKDPRIREAIGLAFDFEWTNQNVMFSAYKRVVSFFQNTPLQAVDKPGPDELA
                     LLEPWRGKIPDSVFGEPYTPPISDGSGSDRALLKQANEMLLAAGCRRDGGVLKLPSGN
                     PFRIEFLDSSKSLQPHTMPFQQNLKKLGISAVPRIVDSSQYKSRTEAFDFDVMSFRLS
                     GTLTPGQSLRELFSSSSATVNGSRNMAGIVDPAVDALVEAVASANTRAALNSAGRALD
                     RLLRAGHYWVPMWYLDSFWAASWNVFSRPERQPKFGVGAPGTWWWDEEKAKKIGL"
BASE COUNT          553 a          835 c          860 g          494 t
ORIGIN      
        1 cgtgatgtgc cagaggtaat cgaggatctt gtgcggccag gagaaatcgg cgaaattgtc
       61 cgaggtgagg ccgcgcagcg ggaacagctg ccagaacgag ccgccgcaaa acagcaccac
      121 cagcaggatc gcaaacagga agctcgggat cgcataggcg aagatcaccg ccgccgaggt
      181 ccagatgtcg aaacgcgagc cgtcggtgac cgccttgcgg atgcccagcg gaacggagat
      241 cgcataggag atcaccgtca tccagagccc gagcgacatc gagaccggca gtttttcctt
      301 gatgagctga acgaccggcg cgttgcggta atagctgtcg ccgaggtgga aggtcgagta
      361 atcgcggacc aggatccaga accgctgcca cgccggcttg tcgaagccgt actgtttctc
      421 gagttccttg atgaatttgg gatcgagccc ctgcgcgccg cgatagcgcg atgaaatgtc
      481 gccgcccgcc tgccccgtcg cgcctgcgcc gatccccccg ccgccggccc cgccgaaatt
      541 cgcggtcgag ccctggtccg tgccctgcaa ctgcgcgatc gcgcgctcca ccgggccccc
      601 gggcacgaat tgcacgatcg cgaacgagac cagcatgacg ccgaagatgg tcggaatcat
      661 caggagaagg cggcgggcga tataggcgag catggattag cgaagcgaat ggcgaatggc
      721 gaacggaaga acgcgaatgg cgagtggcca atggcgaatg gtgagggacg ccattcgcca
      781 ttcgccactc ggcattcgcc tcttctcttt ctcacaggcc aattttcttc gccttctctt
      841 cgtcccacca ccaggttccc ggcgcgccga cgccgaattt cggctggcgc tcgggccgag
      901 aaaacacgtt ccaggacgcg gcccagaacg agtcgagata ccacatgggc acccaataat
      961 ggcccgcgcg cagcagccga tcgagcgcgc ggccggccga gttcagcgcc gcgcgggtat
     1021 tcgccgacgc gaccgcctcg acgagggcat cgacggcggg atcgacgatg cccgccatgt
     1081 tgcgcgaacc gttcaccgtc gccgaagacg aggagaacag ctcgcgcaga ctttgccccg
     1141 gcgtcagggt cccggatagc cggaacgaca tgacatcgaa atcgaaagcc tcggtgcgac
     1201 tcttgtattg tgaggagtcg acgatgcgtg gaacggcgga gatgccgagc ttcttcagat
     1261 tctgctgaaa cggcatcgtg tgtggttgca gcgatttcga ggagtcgaga aactcgatcc
     1321 tgaacggatt gccgctcggg agcttcaaga cgccgccgtc gcgccggcag ccagctgcga
     1381 gcagcatttc gttggcttgc ttcaacagcg cgcggtcgct gccggatccg tcggatatcg
     1441 gcggggtgta aggctcgccg aatacggaat ccggaatttt gccacgccag ggctccagca
     1501 gcgcgagttc gtctggtcca ggcttgtcga cggcctgcaa cggcgtgttc tggaagaacg
     1561 agacgacgcg cttgtaagca gagaacatca cgttctggtt cgtccactcg aagtcgaagg
     1621 cgagaccgat cgcttcgcga atgcgcgggt ccttgaactg ttcgcggcgg gtgttgaaat
     1681 accagccctg cgagccggtg ggcgcgccgt tgtgcagcgt ctccttgacg acgcgcccct
     1741 ccttgacggc ggcgaattcg taattgttcg cccagaagcg cgaggtgtgt tcctcattga
     1801 agttcacgac gccggccttg aaggcttcga aggcgacctg acgatcgcga aaatattcgt
     1861 atcgcagccg ttgaaaattg gctccgccaa cattgagcgg gtggtctttg gcccaataag
     1921 cgtggtcgag ttcgaattcg atgaagcggc tctgttcaaa cgctttgacc ctataggctc
     1981 cggagccgag cggcgcttcg agcgtcgccg cgtcgaaatc ccgacctttc caccatgcgg
     2041 cggagaatac gggcatgccc gcgacgatga gatgcgcatc gcgcgtgcgg ttcttgatga
     2101 agcgtatctt gacgacgtcc tcggcctcgg cttcaacact ctcgacttcg gtcaacaacc
     2161 gcgaaaaata ggggtgcgcc ttctccttca gggtcgtcag cgaaaagacg acgtcggcgg
     2221 cgcgcaacgg cgagccatcg gagaaccggg cttcggggcg cagcagaaaa cgataggtca
     2281 gcttatcctg ggaatagcgc accgagcggg cgatcagccc gtaaaccgag tcgggctcgt
     2341 cttcgttgcc gcccatcagc gaatcgtagg tcgccagcat gcccgccgcg ccgtcgccct
     2401 tcttcgaaaa gacattgagc gtgtcgaagg tctcaaaatt ttgattgccc gtcgtcgtgc
     2461 tcacgagcaa tgccagcagg ccacccttgg gcgcattggg attgacatag gccagcgaca
     2521 tgaaatccgg cggcaacgcc agatccccga aggccgagag cccgtgcgtc tcgatctcgt
     2581 cctcggcccg cgcaagcggc caaccgacgc tcaaagacgc ggcgaggctc gccgctgtgc
     2641 ctttcagcgc cgctcgtctt gaaatgatga tgcccatgag gcttgctccg acgggcgaaa
     2701 tctttcggtg tgattcgccg atgatctaat ctagctttgc cc
//
LOCUS       DASZMC010000629         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2466893,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000629 DASZMC010000000
VERSION     DASZMC010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466893"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..546)
                     /locus_tag="VG274_12770"
     CDS             complement(250..546)
                     /locus_tag="VG274_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07580.1"
                     /translation="MSNASTSPAARGDFETSAGGGHPAPNAIGWLGLAAAPTFAIMAL
                     WTALFGGQPEILCTGMQHASPLNGMALMYALMSTFHSAPWLRLLSRRRNKGRQS"
     gene            complement(539..1303)
                     /locus_tag="VG274_12775"
     CDS             complement(539..1303)
                     /locus_tag="VG274_12775"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HWE07581.1"
                     /translation="MTMHKTGTRDEWLAARLDLLDAEKALTRQSDELAERRQQLPWVR
                     VEKDYSFDTEDGKASLAALFRWRSQLLVYHFMFGPDYKAGCPSCSMIADGFNGFAVHL
                     ANHDVTLMAVSRAPLAKLTAYKQRMGWTFPWASSDGGDFNYDFNVSYTEAQEKEGPVE
                     YNYARGAHAMDMTPVPPPVAQFAATCGVDAPTYRRDRPGMSAFVREGDTVYHTYSTYA
                     RGLDGLWGAYQWLDRAPKGRNEQGIWWRRHDEYGHV"
     gene            1389..2609
                     /locus_tag="VG274_12780"
     CDS             1389..2609
                     /locus_tag="VG274_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07582.1"
                     /translation="MDSLITAAAHALAMGDPLGALNRVALRDDAPALALRGIAMAQLG
                     DLDRAKALVRRAARAFGPREAMARARCVVAEAEIALASRDLAWPTKALAAASATLDAH
                     GDHANAAYAHSLEVRRLILIGRLDDAEHELQERDLSALTPALRTVHEVARAGIAMRRV
                     RARAAHAALARAEEAARWARIPALAAEVEQALAQLDTPVARLIARGEERSLLLEDVEA
                     LIASKALVVDACRNLVREGDVIVPLATRPVLFALARTLGEAWPDDVPRDVLLARAFGA
                     KHADESHRARLRVEIGRLRRLLRALARVNATKRGFLISPRCAPDVVVLARPVEEENAA
                     VLAFLADGESWSSSALALALGTSQRTVQRALDQLAASGKVQAFGCGRARRWTTPPLPG
                     FATTLLLTAPLPGG"
BASE COUNT          474 a          871 c          933 g          486 t
ORIGIN      
        1 cggcatccat tcgagtgtgt tcatatgcac gcgaaatatg cgttcgaagt cgatattacc
       61 cgtcgttgct ggaagcttaa cgtcgaactt gatatgcgcg actgcaacgc gtgtcgcgag
      121 aaagaaatag aatgccacgg cgagcaatgt gacgatcgca gtgtagtgcg gcatggcgac
      181 atcctatggt gaaccggcgg cagcagcggc aggattggaa gggcatactc ggtccggcta
      241 gcttccgaat caagactggc ggcctttatt tcggcgacga gaaagtaatc tcaaccaagg
      301 cgccgaatgg aatgtgctca tcaaggcgta catcagcgcc atgccgttca atggcgatgc
      361 atgctgcatt cccgtacaga gtatctcggg ctggccgccg aacagggcgg tccacagcgc
      421 catgatcgca aaggtcggcg cggcggcaag gccgagccag ccgatcgcat tgggggcggg
      481 atggccgccc ccggcgctgg tctcgaagtc gccacgcgcg gcaggagacg tcgacgcgtt
      541 agacatggcc gtactcgtcg tgccgacgcc accatatgcc ctgctcgttg cgtcccttgg
      601 gcgcgcgatc gagccactga taggctcccc agaggccgtc cagaccacgc gcataggtcg
      661 aataggtgtg ataaaccgtg tcaccttcgc gcacgaaggc gctcatgccg ggccggtcgc
      721 gccgataagt cggtgcgtcg acgccgcagg tcgccgcaaa ttgtgcgacg ggcggcggca
      781 ccggcgtcat gtccatcgcg tgggcgccgc gcgcataatt atattcgacc ggtccttctt
      841 tctcctgcgc ttcggtgtag gaaacgttga agtcgtagtt gaagtcgccg ccgtcagacg
      901 acgcccaggg gaaggtccag cccatgcgct gcttgtaggc tgtgagcttg gcgagcggcg
      961 cacgcgacac cgccatcagc gtgacatcgt ggttggcgag gtggacggcg aagccgttga
     1021 agccgtcggc aatcatcgag catgacgggc agccggcctt atagtccggc ccgaacatga
     1081 agtggtagac gaggagctgc gagcgccacc ggaagagtgc cgcgagcgaa gcctttccgt
     1141 cctcggtgtc gaaagaatag tccttttcca cgcggaccca gggcagttgc tgccgccgct
     1201 cggcgagttc gtcgctttgc cgcgtcagcg ccttttccgc atcgagcaga tcgagccgcg
     1261 ccgcgagcca ttcatcacgt gttccggtct tatgcatggt catcatcatt ctccgttctt
     1321 agtgccgatg gagttagact aggacttccc gtggggaccg taggagtgac aagtatgacg
     1381 cgattgcgat ggactcgctg atcacggcgg cggcgcacgc gctcgccatg ggcgatccgc
     1441 tcggcgcgct gaacagagtg gcgttgcggg acgacgcgcc ggcactggcg ctgcgcggca
     1501 tcgccatggc gcagctcggc gacctcgacc gcgcgaaagc gctggtgcgg cgcgcagcgc
     1561 gtgccttcgg cccgagagag gctatggcgc gcgcgcgatg cgtcgtcgcc gaggctgaga
     1621 tcgcgctcgc gtcgcgcgac ctcgcttggc ccacaaaggc gcttgccgcg gcgtcggcta
     1681 ctctcgatgc gcatggcgac cacgccaacg ccgcctatgc acactctctt gaagtccggc
     1741 gcctcatcct gatcggccgt ctcgacgatg ccgagcacga acttcaagag cgcgatcttt
     1801 cggctcttac gccggcgctg cgcaccgtcc atgaggtcgc acgtgccggg attgccatgc
     1861 ggcgcgtccg tgcgcgcgcg gcccatgcag cgcttgcccg tgccgaggag gccgcgcgct
     1921 gggcgcgcat tccagcgctc gctgcggagg tcgaacaggc tctcgctcaa ctcgacacgc
     1981 ctgtggcgcg gttgatcgcg cgcggcgagg agcgttcgct tctattggag gatgtcgagg
     2041 cgctcatcgc gtcgaaggcg cttgtcgtgg atgcctgccg caatctcgtg cgcgagggcg
     2101 acgtgatcgt gccgctcgcg acgcgcccgg tgctgttcgc gctggcgcgc acgcttggcg
     2161 aggcatggcc cgacgacgtg ccgcgcgacg tcttgcttgc ccgcgccttc ggcgcgaaac
     2221 acgccgacga atcgcatcgc gcgcggctgc gggtcgagat tggccggctg cgccggctgc
     2281 tgcgagccct cgcgcgcgtg aacgcgacaa agcggggttt cctgatctcg ccgcgctgcg
     2341 cgcccgacgt ggtcgtgctg gcgcgtccgg tcgaggagga aaatgccgcc gtgcttgcct
     2401 tcctcgccga tggcgagtcg tggtcgagct ccgcgcttgc gctcgcgctc ggcacgagcc
     2461 agcgcacggt gcagcgggcg ctcgatcagc ttgccgcatc gggcaaagtg caggccttcg
     2521 gctgcggccg ggcgcggcgc tggacgacgc ccccgctgcc cggattcgcg accaccttgt
     2581 tactcacggc gccactgccg ggcggttaaa acggttgcag gaaaagtgaa cgcgggtttc
     2641 cgtccagagc cgtggcaaag cggagagaag caaaatgaaa acatcgaagg cagacatcat
     2701 ccgcgaatat gggccgttcc cggaaaccga acgcgttaac ggcgtcagct atgacggcaa
     2761 gcag
//
LOCUS       DASZMC010000630         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4121876,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000630 DASZMC010000000
VERSION     DASZMC010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4121876"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..659
                     /locus_tag="VG274_12785"
     CDS             102..659
                     /locus_tag="VG274_12785"
                     /inference="COORDINATES: protein motif:HMM:NF008517.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HWE07583.1"
                     /translation="MVTVDPKSTALVLIDLQNGILGFPLAPLSGPELLNQGKALAQKF
                     RAAGAPVVLVNVAWASDSKDVLRAPVDYPTHPEGGLPAGFSNLADGLAADGDILITKR
                     QWGAFTGTELDLQLRRRGISTIVLGGVSTNMGVESTARQAWELNYGVVIASQATTSID
                     GDMHSFAMGRIFPLIARVADDIAFG"
     gene            854..>1755
                     /locus_tag="VG274_12790"
     CDS             854..>1755
                     /locus_tag="VG274_12790"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWE07584.1"
                     /translation="MSGASQSRNPLFNKNRMKVGTFATNTIGSVHTSAPDAYEATWEN
                     SLRAARAADAAGFEALLALARWKAVAPGPMDHRGGIVLDPFTWAAGIAMGTRYSAVFA
                     TSHAPTVHPLIVAKQSATIDHISGGRFGLNVVGGWNRREFDMFGLTLAEHDARYDYLD
                     EWMEVLERLWSGTEEFDFAGKFLNLKGAMSMPQPIQQPRPVIMNAGISRRGQQFACEH
                     ADCCFVAPGLGKTEIEGYKTMAREEFGREVGVWMQVPIAQRNTRQEAEDYLNYFAVQH
                     EDTPTVDAWLAGMAKESRSLNDEQA"
BASE COUNT          348 a          556 c          557 g          294 t
ORIGIN      
        1 ctgccctctc ccctcaaaag cgtgaggaaa ccggcgcggc gcggcgcggt acatagccac
       61 gccgaaataa tccggttttc attcacgctc aggagcactt catggtcacc gtcgatccca
      121 aatccaccgc gctggtgctc atcgacctgc agaacggcat tctcggcttc ccgttggcac
      181 cgctttccgg accggagctg ctgaaccagg gcaaggcgct cgcccagaaa ttccgagccg
      241 cgggtgcgcc tgtcgtcctg gtgaatgtcg catgggcctc ggattccaag gacgtcctgc
      301 gtgcgcccgt ggattatccg acccaccccg agggcggcct gccggccggc ttcagcaacc
      361 tcgcggacgg tcttgccgcc gacggcgaca tactgatcac caagcggcag tggggtgcgt
      421 tcaccggcac cgaactggac ctgcagctcc gccggcgcgg gatcagcacg attgtgcttg
      481 gcggcgtctc gaccaacatg ggtgtggaat ccacggcgcg gcaggcctgg gagctcaatt
      541 atggcgtggt tatcgctagc caggcaacga cgagcatcga tggcgacatg catagtttcg
      601 ccatgggccg gattttcccg cttatcgcgc gcgtcgccga cgacatcgct ttcggctgac
      661 cgggacggcg ggcgaggcgc cctcggttcg aggcgccctg cccgtcagcc cccgcacttc
      721 cgataggatg gcagagtttc cgctggcaaa gccatcaccg gaggcggaaa attctcgaac
      781 gcgcaagcag cagccgacct gaagcggccc acgccgaact gcatgcgaaa ggtgtgagcg
      841 agaggaaccc accgtgtctg gagcaagcca aagccgcaac ccgctgttca acaagaaccg
      901 gatgaaggtc ggcacctttg ccacgaacac gatcggttct gtgcatacct cggctccgga
      961 cgcctacgag gcaacttggg aaaacagcct gcgcgccgcg agggcggccg acgcggcggg
     1021 cttcgaggcg cttctcgccc tggcgcgatg gaaagccgtg gcacccggcc cgatggacca
     1081 tcgcggcggc atcgtgcttg acccgttcac atgggcagct ggaatcgcaa tgggcacccg
     1141 ctattctgcg gtcttcgcca caagccacgc gccgacggtc catccgctga tcgtcgcaaa
     1201 gcagtccgcg acaatcgacc atatctcggg cggacgtttc gggctcaacg tcgttggcgg
     1261 ctggaacagg cgggagttcg acatgttcgg actgacgctc gccgagcatg atgcacggta
     1321 tgactatctc gacgaatgga tggaggtcct cgagagactg tggtcgggga cggaagaatt
     1381 cgattttgcc gggaaattcc tgaatctgaa gggggcaatg tcgatgccac agcccattca
     1441 gcagcctcgg cccgtcatca tgaacgccgg catatcgcgg cgcggccagc aattcgcctg
     1501 cgaacatgcg gattgctgct tcgtcgcgcc cggactgggg aagacggaga tcgaaggcta
     1561 caagacaatg gcccgcgagg agttcggccg cgaagtcggc gtctggatgc aagtgccgat
     1621 cgcccagcgc aatacacgcc aggaggccga agattatctc aactacttcg ccgtgcagca
     1681 cgaagacacc ccgacggtgg acgcatggct cgctggaatg gcgaaggagt cgcgcagcct
     1741 gaacgatgaa caggc
//
LOCUS       DASZMC010000631         5458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2468357,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000631 DASZMC010000000
VERSION     DASZMC010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468357"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VG274_12795"
     CDS             <1..384
                     /locus_tag="VG274_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWE07585.1"
                     /translation="RQSGFKIAHEGARVVCEAVRGPKGLQARKLLLLDNSMAPAEARP
                     PQRPARFTVAPRGPAFEATVKWFNRGKGYGFVSRGQETPDIFVHMELLRRSGIRELNQ
                     GQRVRVRVGDGPKGELAAEIAVLEG"
     gene            440..931
                     /locus_tag="VG274_12800"
     CDS             440..931
                     /locus_tag="VG274_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWE07586.1"
                     /translation="MKRKHLFAVAMLVCLPGSLAASSAAPNAKTVLPIDTIVIDADHG
                     AVRFSAEIAADEASQEKGLMYRTRLAPDAGMLFDFHAPEPQTFWMKNTVLPLDMIFIR
                     ADGTISSIAPDAVPYSETPIPSTEPVRAVLEINAGRAAALGIEPGQRVHSAIFGNALP
                     GRK"
     gene            1007..1083
                     /locus_tag="VG274_12805"
     tRNA            1007..1083
                     /locus_tag="VG274_12805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1041..1043,aa:Pro,seq:tgg)
     gene            1109..1414
                     /locus_tag="VG274_12810"
     CDS             1109..1414
                     /locus_tag="VG274_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HWE07587.1"
                     /translation="MRARIYRPSKTAMQSGRALTKKWVLEFEPESPRTIEPLMGWTSS
                     ADMRSQILLEFESRDEAVAYAMTHEIPYQVFEPDLPRSRPKSYSDNFRFDRKEPWTH"
     gene            1485..1561
                     /locus_tag="VG274_12815"
     tRNA            1485..1561
                     /locus_tag="VG274_12815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1519..1521,aa:Arg,seq:tct)
     gene            1604..2908
                     /locus_tag="VG274_12820"
     CDS             1604..2908
                     /locus_tag="VG274_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HWE07588.1"
                     /translation="MSVIGGKWSNWSGGVSCKPRQILSPQNEIELAASIRNAEGPVRA
                     PGSGHSFTPLCASDGALLVLDAFKGLKATSRDESLATIFAATPLWAIGPALHAHGLAL
                     RNMGDIDRQTLAGVVATGTHGTGRTLGSLSSDVAGFRLILANGDVLNCTPTENAEIFL
                     GGRVAVGTLGVMTEISMALRPRYRLIENNFLLAPEELFRRLDQLVSTNRHFEFFWFPY
                     AGVAVCKTLNETDKPAPAPRSADFMRRRGERVGGEARVFAGINEILPGLPVLLKPAHG
                     LFSRLMPNAASARWSHEIFPSPRPIRFNEMEYAVPLARGADCIREIVAEIRRKRINTS
                     FPLEFRTVAEDDIWLSPFYRRESATIAVHQYYKVDTGRLFDACEAIFRLYEGRPHWGK
                     RHTNSREELAALYPAFERFRSLRRELDPKGKFLNSYLRSIFE"
     gene            complement(2913..3569)
                     /locus_tag="VG274_12825"
     CDS             complement(2913..3569)
                     /locus_tag="VG274_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWE07589.1"
                     /translation="MTHMAEPHYFTESAVQVWTRIGARHRIRDEYFLRAVEHYFRPGP
                     ILEIGAATGHLSEILLRHGFDVTASDVSPRFVAAIQARGVRAQLVDATANIRAQTGRS
                     FANVLAQGVIPLVRRDPATANATLAAIHAALEPRGRLICISPHAWRDPDPQSYFRPRE
                     QIEIARASGLYRIIKVLPHQVVPTALYRSWNAGILNFLDFHAARLAAVRLVWIAEKIG
                     "
     gene            complement(3566..4408)
                     /locus_tag="VG274_12830"
     CDS             complement(3566..4408)
                     /locus_tag="VG274_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07590.1"
                     /translation="MLSSLRHRVYQDFFMGSRLAEYRSLLAHIKELGYEFRTMAEFAE
                     AAGRGGAPVRPACLLRNDVDSDPGGAARMFACDREAGVRATYYFRLATIDPPLMQEIV
                     RHGGEVGYHFEEIASVAKRLGLRSGRQIDAGLDLMRREFRDNVLRFRARSGVMPRTVA
                     SHGDFANRRIGVPNHHLLTRTLMDDLGIVADAYDPRIHAGLDARYTDCPPPEWWRPQN
                     PLLGLSRRPRTVSILVHPRQWTCAPALNLRLAAERLWEEGAWRWNDIMASHRQRESRS
                     SAET"
     gene            complement(4423..>5458)
                     /gene="metC"
                     /locus_tag="VG274_12835"
     CDS             complement(4423..>5458)
                     /gene="metC"
                     /locus_tag="VG274_12835"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HWE07591.1"
                     /translation="SGDAPYVYGRLGTPTSRSLEEAVTALEGGARTVLCPSGTNAITT
                     AILSVCGSGDHLLMTDNCYGPTRSFCENSLKRLGVETGFYDPLIGGGVEELFRSNTRA
                     VFCESPGSLTFEVQDVPAIAYVAHARGASVLLDNTWATPLCFDAFAHGADLSIQAATK
                     YVGGHADVMLGYVTANTAHAEQLADTHAGLGLCASGDDCFLALRGLRTMPVRLKRHEE
                     TGLKLARWLESRSEVARVLHPALESDPGHPIWKRDFTGARGLFGVELAPVAKTALAAF
                     FDGLECFGIGYSWGGYESLIVPAHFRRSARPFVARGPLLRIHAGLEDANDLLADLANA
                     FARMQTPTAA"
BASE COUNT         1064 a         1661 c         1741 g          992 t
ORIGIN      
        1 cggcagtccg gattcaaaat tgcgcatgag ggcgcaagag tcgtttgcga ggccgtcagg
       61 ggaccgaaag gcctgcaagc gcgaaagctg ctgttgctcg acaattcgat ggcgccggcc
      121 gaagctaggc cgccgcagcg cccggcaagg ttcaccgtcg cgccgcgcgg tcctgcattc
      181 gaagcgaccg tgaagtggtt caatcgcggc aagggctacg gattcgtctc gcgcggccag
      241 gaaacgccgg acattttcgt tcacatggaa ctcctccgcc gcagcggtat ccgcgaattg
      301 aaccaggggc aacgtgttcg cgtgcgagtg ggcgacgggc ccaagggaga gctcgcggcg
      361 gaaatcgcgg tgcttgaagg atagctcgac cgccgcggcc tgtggcagta tccgtgcgtc
      421 tctcacggct cagctccaaa tgaagcgcaa acacctgttc gccgtcgcca tgcttgtgtg
      481 cctgccggga agcctcgctg cgagctccgc agcgccaaac gcaaaaaccg tcctgccgat
      541 cgatacgatc gtgatcgatg ctgatcacgg cgctgtccga ttttcggcgg aaatcgcggc
      601 ggatgaggcg tcgcaggaaa agggcctgat gtaccgcacc agacttgcgc ccgatgccgg
      661 catgctgttc gattttcacg cgccggaacc ccagactttc tggatgaaga acacggttct
      721 cccgctcgac atgattttca ttcgcgccga cggcaccatt tcctccatcg cgcccgacgc
      781 cgtgccctat tcggagacgc ccattccctc caccgagccg gttcgcgcgg tgctcgagat
      841 caatgccggg cgcgccgcgg cgctcggtat cgagcctgga caacgcgttc acagtgcaat
      901 tttcggaaac gcgctccccg gccggaagtg aaactgcgtt attgaagcag cggaagcgaa
      961 gttgtaaggg acgacgcctt tcggcggccg gagttccgac tgagaccggg gcgtggcgca
     1021 gcctggtagc gcatctgctt tgggagcaga gggtcgcgtg ttcgaatcac gccgccccga
     1081 ccagccggaa gacctgcggg agtaggcaat gcgggcgaga atctatcgtc cctcgaagac
     1141 ggcgatgcag tcgggcaggg cactgacaaa gaagtgggtg ctggaatttg agcccgagag
     1201 cccccgcacg atcgagcccc tgatgggctg gaccagttcc gccgacatgc gcagccagat
     1261 cctcctcgaa ttcgagtccc gggatgaggc ggtggcctat gccatgacgc acgagattcc
     1321 ctatcaggtg ttcgagccgg atctgcccag gagccgtcca aaatcctatt cggacaattt
     1381 tcgctttgac cgcaaggagc cctggacgca ctgaggcaag ccggcgaaag acccgcagca
     1441 gtttttgatt tgcgtttcgg ttgctacata cttgccggcg cagaggcccc gtagctcagc
     1501 ggattagagc aattgccttc taagcaatgg gtcgcaggtt cgattcctgc cggggtcacc
     1561 attccagttt gtttgcccaa acggcagagc gaatcctcga ggagtgagcg tcataggcgg
     1621 caagtggtcg aattggtcgg gcggcgtgag ttgcaagcca cgccagattc tctcgccgca
     1681 aaacgaaatc gaactggccg cgtcgatccg caacgcggaa gggccggtgc gtgcgccggg
     1741 cagcggccat tcctttacgc ctctgtgcgc gtccgacggc gcgctgctcg tgttggatgc
     1801 gttcaaaggc ctgaaggcga cgtcccggga cgagtcgctg gcaacaatct tcgcggccac
     1861 gccgctatgg gctatcggcc ccgccttgca tgcgcatggg ctggcgctca ggaacatggg
     1921 cgacatcgat cgccagacgc tcgccggcgt cgtcgccacc ggcacgcacg gaacgggacg
     1981 gacgctgggt agtctttcgt ccgacgtagc cggattcaga ctcattctcg caaacggcga
     2041 tgtcctgaat tgcacgccca ccgagaacgc ggaaatcttt ctcggcggtc gggtggccgt
     2101 cggaactctg ggagtcatga cggaaatctc gatggctctg cggccgcgct accgtctgat
     2161 agaaaacaat ttcctcctgg cgcccgagga attgtttcgc agactggacc agctggtttc
     2221 gaccaatcgc catttcgagt tcttctggtt tccctatgcc ggcgtggcgg tctgcaagac
     2281 gctgaacgag accgacaagc cggcgcccgc accgcgcagc gcagatttca tgcgcaggcg
     2341 cggcgaaagg gttggcggag aggcgcgtgt gtttgcgggc atcaacgaga ttctgcctgg
     2401 gctgccggtc ctgctcaaac cggctcatgg actgttctcg cgcctgatgc ccaatgcggc
     2461 gagcgcgcgc tggagtcatg aaatctttcc cagtccgcgg cccatccgct tcaacgagat
     2521 ggagtacgca gtccctctgg cgcgcggcgc cgactgcatt cgcgaaatcg tcgccgagat
     2581 acgccgcaag cggataaaca ccagtttccc gctcgaattc cgcaccgtcg ccgaagacga
     2641 tatctggctc agccctttct acaggcgcga gagcgccacc attgccgtac atcaatacta
     2701 caaggtcgat accggcagac tcttcgacgc ctgcgaagca atctttcggc tctacgaggg
     2761 ccgtccgcat tggggcaagc gccacacaaa ttcgcgggag gaactcgcag ccttgtatcc
     2821 ggcgttcgag cgattccgct cgctcaggcg cgaactggat ccgaaaggaa aatttcttaa
     2881 ctcatatttg agatcgattt ttgaataacg tttcagccga tcttctcggc gatccacacg
     2941 aggcgcacgg cggcgagtct tgcggcgtgg aagtcgagga agttgagaat tcccgcattc
     3001 cagcttctgt agagcgccgt cggaaccacc tggtgcggaa gcaccttgat gatgcgatag
     3061 agcccggatg ccctggcaat ctcgatctgc tcgcggggac gaaaatacga ttgcggatcg
     3121 ggatcgcgcc aggcgtgcgg gctgatgcag atcaggcgtc cccggggttc gagcgcggca
     3181 tgaatggcag ccaacgttgc gttcgctgtg gcaggatcgc gacgcacgag cggaatgacg
     3241 ccctgcgcca gaacgttggc aaaggatctg cccgtctgcg cccggatatt cgcggtcgca
     3301 tcgacgagtt gggcccgcac gcctcgcgcc tggatggcag caacaaatcg gggagagaca
     3361 tccgatgccg tcacgtcaaa tccgtgacgc agaaggattt ccgacagatg tccggtcgca
     3421 gccccaatct cgagaatggg ccccggtcgg aaatagtgtt cgaccgcacg gaggaaatat
     3481 tcatcgcgga tgcgatgtcg ggcgcctatc cgtgtccaga cctgcacggc gctttcagta
     3541 aaataatggg gctccgccat atgggtcatg tctctgcaga cgaccgcgat tcgcgctggc
     3601 ggtgcgacgc catgatgtca ttccatcgcc atgcgccttc ctcccacaat cgctcggcgg
     3661 caagcctgag attcaaggcc ggagcgcagg tccactgcct cggatgtacc aggatcgaga
     3721 ctgtccgggg acgccgcgag agcccgagca gcgggttctg gggacgccac cattcgggag
     3781 gcgggcagtc ggtgtatcgc gcgtcgagtc cggcgtgaat ccggggatca taggcgtcgg
     3841 cgacgatacc aagatcgtcc atcagggtcc ttgtcagcag atgatgattg ggaacgccga
     3901 tgcggcggtt ggcgaaatcc ccatgcgacg ccacggtgcg cggcatcacg ccggagcggg
     3961 cgcgaaagcg caagacattg tcgcgaaatt cgcggcgcat cagatccagg cccgcatcga
     4021 tctgtcttcc ggatcgcaat ccgaggcgct tggcgacgct ggcgatttcc tcgaaatgat
     4081 agcccacctc gccgccgtgg cggacgattt cctgcatgag gggcggatcg atggtcgcga
     4141 ggcggaaata ataggtcgcg cgcacgcccg cttcgcggtc gcacgcgaac atgcgcgcgg
     4201 cgcccccagg gtcgctgtcg acgtcgtttc gcaacaggca ggccggtcga accggtgcgc
     4261 cgccgcgacc ggccgcttcg gcgaattccg ccatcgtgcg gaactcataa ccaagttcct
     4321 tgatgtgggc gaggaggctg cgatattcgg cgagccggct tcccatgaaa aaatcctggt
     4381 acacgcgatg gcggagggac gagagcacgg ggaaattcgg tactatgccg ccgtcggcgt
     4441 ttgcattcgt gcgaatgcgt tcgccagatc ggcgaggaga tcgtttgcgt cttcgagccc
     4501 ggcgtgaatt cgcagcaatg gtccccgcgc gacaaatggc ctggccgagc gacgaaaatg
     4561 ggcgggaacg atgagacttt catatcctcc ccaggaatag ccaatgccga aacattcgag
     4621 cccgtcgaag aaggctgcaa gcgccgtctt ggcgacaggc gcgagctcca ccccgaacaa
     4681 tccgcgggcg ccggtgaagt cgcgcttcca gatcggatgg ccgggatcgc tttccagcgc
     4741 gggatgcagc acgcgcgcca cttcggaacg actctcgagc cagcgggcga gcttgagtcc
     4801 cgtttcctcg tggcgcttca ggcggacagg catggtgcgc agtccgcgca gggcgaggaa
     4861 gcaatcgtct ccgctcgcgc acagcccgag acccgcatgg gtatccgcca attgctcggc
     4921 atgcgccgta ttggccgtta cataaccgag catcacatcg gcgtggccgc cgacatattt
     4981 ggtcgccgcc tggatcgaca ggtcggcgcc gtgcgcaaac gcgtcgaaac agaggggcgt
     5041 agcccaggtg ttgtcgagaa gaacggaagc gcctcgcgcg tgagccacgt atgcgatcgc
     5101 gggcacgtcc tgcacttcga aggtcaggga tcccgggctc tcgcagaaca ccgccctggt
     5161 gttcgagcgg aacaattctt cgacgccgcc gccgattaac ggatcgtaga aacccgtttc
     5221 gacgcccagc ctcttgaggc tgttctcgca aaaactgcgc gtcggtccgt agcaattgtc
     5281 cgtcatgagc agatgatcgc cggaaccgca gacggacagg atggcggtcg tgatcgcatt
     5341 ggtgccggaa gggcacagca cggtgcgcgc gccaccctca agggcggtga ccgcctcttc
     5401 gagacttctg gatgtcggcg tgccgaggcg gccatagaca taaggcgcgt cgcccgat
//
LOCUS       DASZMC010000632         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1899954,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000632 DASZMC010000000
VERSION     DASZMC010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899954"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VG274_12840"
     CDS             complement(<1..892)
                     /locus_tag="VG274_12840"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWE07592.1"
                     /translation="MIAGKPKTMIVAGVMSGTSADGVDVAICRVSPAEGRARVKVLGH
                     HAVAYPARLRKAVLAAMNSKAAKSTSTTELARLNWRLGEVYAEAVAAAVRKIGVKPQL
                     IACHGQTIYHQGAAIKYLGAPVRCTWQTGEAAVIAERMRVPVVSDFRPADMAAGGQAA
                     PLVPMFDFCMFRDANKSRVLLNLGGIANVTALPAACRAEDVMAFDTGPANMVIDACMQ
                     RLFARGFDRNGATAARGRALEPVLTKVLRARYFSAPPPKSCGREEYGEPFVAQFIAAC
                     GRQSSHKEDAVATATALTART"
     gene            complement(889..>2289)
                     /gene="mutS"
                     /locus_tag="VG274_12845"
     CDS             complement(889..>2289)
                     /gene="mutS"
                     /locus_tag="VG274_12845"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWE07593.1"
                     /translation="MIARTLVEEPPVSLNDGGAVREGVDAELDELRGLSQNGRRMIAA
                     IEERERERTGIGSLKVRFNSVFGYYIEVTKANSKSVPADYERKQTLVNAERFTTPELK
                     ELETKILTAQERSGEIERRIFAELRRQLLASAPRVRETARHVAEIDLLACFAHVAAMR
                     GWVRPQVDASGLMEVIAGRHPVVERRLEESGGGRFVPNSVYLCAGETCEGPSLLLITG
                     PNMGGKSTYLRQAALLTILAQCGSFVPAERMRLGLVDRIYTRIGASDNVARGRSTFMV
                     EMTETAAILNTATERSLVLLDEMGRGTATYDGLSLAWATVEHLHDRIGARTLFATHYH
                     ELTLLSERLAAMQNARVTVREGAGGIVFLHAVEAGAANKSYGIEVARLAGLPQAVVER
                     AREVLKLHERAESQQVREAVPTKERLAALQMTMFTPLSQRIVDRVEALDVDGLTPREA
                     LQILAELQRELKGGAA"
BASE COUNT          406 a          865 c          602 g          416 t
ORIGIN      
        1 tcgtcctcgc agtcaaggct gttgccgtgg cgacggcatc ctctttgtgg gaactctggc
       61 gcccacacgc ggcgatgaac tgcgcgacga acggctcacc atattcctcg cgcccgcacg
      121 atttcggcgg tggggcagag aagtatcgcg cccgcagcac cttcgtgagc accggctcca
      181 gcgcgcgacc gcgcgccgca gtcgccccgt tgcggtcaaa cccacgtgca aacagccgct
      241 gcatgcacgc atcgatcacc atgtttgccg gcccggtgtc gaaggccatc acatcctcag
      301 cgcggcacgc tgcaggcagt gccgtcacat tcgcgatgcc accgagatta agcagcactc
      361 ggctcttgtt cgcgtcgcga aacatgcaga agtcgaacat cggtaccagc ggtgccgcct
      421 gtccacctgc agccatatcc gcgggacgaa agtcactcac cacaggaaca cgcatgcgct
      481 cggcgatcac cgccgcctcg ccggtctgcc acgtgcatcg caccggcgct ccaagatact
      541 ttatcgccgc gccctggtga tagatcgtct gcccgtgaca cgcgatcaac tgcggcttca
      601 cgccaatctt gcgcacggcc gctgcgacgg cttccgcgta cacctcgccg agtcgccagt
      661 tcagcctggc cagctctgtg gtgctcgtgc tcttcgcagc ttttgaattc atcgcggcga
      721 gcaccgcctt ccgcagtctc gccggatacg ccaccgcatg atgccccagt accttcacac
      781 gcgcgcggcc ctccgctggt gacacgcggc agatggcgac atcgacacca tccgctgagg
      841 tcccgctcat cacacctgca acgatcatcg tctttggctt gcccgcgatc acgccgcacc
      901 gcccttcagc tcgcgctgca actccgccag aatctgcagc gcctcacgcg gcgtcagccc
      961 gtccacatcc agcgcctcca cacgatcaac aatgcgctgt gacagcggcg taaacatggt
     1021 catctgcaat gcggccagac gctccttggt cggcacagcc tcgcgaacct gctgcgactc
     1081 tgcgcgctca tgcagcttca gcacctcgcg cgcacgctca actacagcct gcggcagccc
     1141 tgccaaacgc gcgacctcaa tgccataact cttgttcgcc gcgcccgctt ccaccgcgtg
     1201 cagaaacacg atcccgcccg cgccctcccg tacggtcacg cgagcattct gcattgcagc
     1261 cagccgctca ctcagcaggg tcagctcgtg atagtgcgtc gcaaacagtg tgcgtgcacc
     1321 gatgcgatcg tgcaggtgct ccaccgtcgc ccatgccagg gacaagccgt cgtacgttgc
     1381 cgtgccgcgc cccatctcat ccagcagtac gagcgagcgc tccgtcgcgg tattcagaat
     1441 cgctgccgtt tcggtcattt ccaccatgaa cgttgagcgg ccgcgcgcca cattgtcgct
     1501 cgcgccaatg cgcgtgtaga tccggtccac cagccccagc cgcatgcgct ccgcgggtac
     1561 gaacgacccg cattgcgcca ggatcgtcag cagtgccgcc tgccgcaggt acgtactctt
     1621 gccgcccatg ttcggtccgg tgatcagcag cagcgaaggg ccttcgcacg tctcgcccgc
     1681 gcacaggtac acggagttcg gcacaaatcg gccgccgccc gattcctcca gcctgcgctc
     1741 gaccactgga tgccgccctg caatcacctc catcaaacca ctcgcatcca cctgcggccg
     1801 cacccaccca cgcatcgcag ccacgtgtgc gaagcatgcg agcagatcga tctctgcaac
     1861 gtgccgtgcg gtctcacgca ctcgcggcgc gctcgccaac aattgccgac gcaactccgc
     1921 gaagattctt cgctcgattt cgcctgaccg ctcctgcgcg gtgagaatct ttgtctccag
     1981 ctctttcaac tccggcgtgg taaagcgctc cgcattcacc agcgtctgct tgcgctcata
     2041 gtctgcggga acagacttcg agttcgcctt cgtcacctcg atgtagtacc caaacaccga
     2101 gttgaaccgc accttcagcg atccaatccc cgtccgctcg cgctcgcgct cctcaatcgc
     2161 cgcgatcatt ctgcgcccgt tctgtgagag gccgcgcagc tcatccagct ctgcatccac
     2221 gccctcgcgt actgcaccgc cgtcattcag cgaaaccggc ggctcctcaa cgagcgtgcg
     2281 cgcaatcat
//
LOCUS       DASZMC010000633         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2468517,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000633 DASZMC010000000
VERSION     DASZMC010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468517"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2809
                     /locus_tag="VG274_12850"
     CDS             <1..2809
                     /locus_tag="VG274_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWE07594.1"
                     /translation="KVTLVAGANQGRGGRGGRGGGGGGRGGAALAASRPTSQPAVPYS
                     GLTGEEAEGGVKVTAVVENGPAAKAGIKEGDLLIGADDKKIKTFEELTQEIRSRKVGD
                     TLELKLKTGDKTSTVEVTLGERPQQGAEASNTRPNGGQYGGQIENVQDDQGPSSFEYG
                     GIYKSTDAGESWKRINSLNPRPMYFSLLRVDPSDDRYLYVGGVSMYRSTDSGKTFKPD
                     AGRGVHADQHALWVDPKNGKHMLVGCDGGFYVTYDRTRNWDQLNTVAIGQFYHVSIAP
                     TKPYRVAGGLQDNGSWMGPAISLNGSGPINEDWINIGGGDGFQCQVDQDDPDVVYSES
                     QDGAMSRRNLRTGERGGIRPTTRAAQGGRGRGGGGVGGGAGGAAGGGGGFARGGGGGR
                     GGGYNFNWNTPFVLSHFNSHILYAGGDYVFRSVNRGDTLDIASPKIILTQFGSATALS
                     ESPRNADVVYAGTDDGALWVTTDGCKNWKRVDANVGLPGPRWVGAIEASHYVDGRCYV
                     VFDAHRSNDDNPYVYVTEDFGKTWKPLTANLPWGSTRCISEDIVNPNLLYVGNEFGAW
                     CSLDRGKYWNKLGGNLPTVAVFEFAQHPVNGQIVAATHGRSLWVLDVSALRQIKPEYL
                     ADAPELYEPAPAVRWHSEPSRGGTNRKFVGQNPVFGVEMFYSLPRDAKKATLKVLDVS
                     GKALREFPARSEAGLHRIAWDLRLGGAGGRGGFGRGGGFGGGGGGGFGAAAAGGADAS
                     AATTTGRRGRGGRRGGATAAAPDAGEAAAPGAEQTPAEPANTGGEGGGESQPVAARGG
                     GGRGFVGGGGGGFGGRGGRGGGSVQAGSYRIVLTVDGKEFAQNLRVENDPAVSDSVAM
                     ASNSPDFGEEEEDEDSYFKIDGADEDADMDADEEMNNAGEQSKRERDRDADRDRERDA
                     DKLVAVRQQVTGFAQKLGTRK"
     gene            2806..3408
                     /locus_tag="VG274_12855"
     CDS             2806..3408
                     /locus_tag="VG274_12855"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HWE07595.1"
                     /translation="MKIVRIALGASVVAAMGLSLAAADPAPQKGPRKDPQASYEPRSG
                     PGEGQKYLQRFVGKWTVVKTFYPRNGDPSVAKGDCVQTMINDGRFLKSEFTLHAEAGD
                     TTGFGLIGFEPETGKFTSVWADSRQTRMSFRQGNEPFDGKEIHLVAATVDPSAKPPRP
                     SHTVSRISDDGNTITHKQFASGADGKDRLMMELVLTRKAE"
     gene            complement(3420..4613)
                     /locus_tag="VG274_12860"
     CDS             complement(3420..4613)
                     /locus_tag="VG274_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE07596.1"
                     /translation="MGNELLINREKGGIAMLTPGKILSWVFAVILGVSGAAKAQQPKP
                     IDKVQGVTGRFQVILTERSPLSNATDIAKHTSQKAGPPEPDYDLPKEIFEAYVPKAAG
                     DGGKYGLMVPMPMPNHGFPPAAWLDVLEKHHLIWLGDQNGGINPTASIGLALDAVHNA
                     EKTWPIDPARVYICPAGTKSLAQGIALYYPEIFQGQLCSAVGYWFDKVTDIRTRRFFN
                     VGILNQPTPAQLTLAKSRTRNFFAGRESPDPAHTDLMQLLGEQYTRAGFKHVKYLLVP
                     QSDMTLWTHYPASWFEQGVEFLDAGATEANAPSVAATPATAAPKPAVDAGSAATSTTP
                     TPATPTPDDGEAKASAALRLAKSYINAAKYDTARAKLQALIQSYPKTASATEAKSLLA
                     EIKDK"
     gene            4963..5044
                     /locus_tag="VG274_12865"
     tRNA            4963..5044
                     /locus_tag="VG274_12865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Leu,seq:gag)
BASE COUNT         1041 a         1645 c         1686 g          958 t
ORIGIN      
        1 caaggtgacg ctcgtcgccg gcgccaatca gggccgcggc gggcggggtg ggcgcggcgg
       61 tggtggcggt ggacggggcg gcgcggcgct ggctgcgtcg cgtccaacca gccagccggc
      121 ggttccctat tccggtctca ccggggaaga agccgagggc ggcgtgaaag tcaccgcggt
      181 tgtcgaaaac ggtccggcgg ccaaagcggg aatcaaagag ggtgatctcc tcattggagc
      241 ggatgacaag aagatcaaaa ccttcgaaga gctgactcag gaaatccgtt cacgcaaagt
      301 cggcgatacc ctcgagctga agctcaagac cggcgacaag accagcacgg tggaagtcac
      361 gctcggcgag cgcccgcaac agggtgccga ggcatccaac acccgcccca acggcggaca
      421 gtacggcggg cagatcgaaa acgtgcagga tgaccaggga ccgagcagct tcgaatacgg
      481 cggcatctac aaatcaaccg acgccggcga aagctggaag cgcatcaaca gcctgaatcc
      541 gcggccgatg tatttcagcc tgctgcgcgt cgacccgagc gacgaccgct atctctacgt
      601 cggcggcgtt tcgatgtatc gatcgaccga cagcggcaaa accttcaagc ccgatgccgg
      661 ccgcggcgtg catgccgacc agcatgccct ctgggttgat ccgaagaacg gcaagcacat
      721 gctcgtcggc tgtgacggcg ggttctacgt cacctacgac cgcacgcgca attgggatca
      781 gctcaatacc gtcgcgatcg ggcagtttta tcacgtgtcg atcgcgccga cgaaacccta
      841 tcgcgttgcc ggcgggttgc aggacaacgg ctcttggatg ggccccgcga tctcgctgaa
      901 cggcagcggg ccgatcaacg aagactggat caacatcggc ggcggcgacg gattccagtg
      961 ccaggtcgac caggatgacc cggatgtcgt ctactccgaa agccaggatg gcgcgatgtc
     1021 gcgccgaaac ctccgcaccg gtgaacgggg cggaatccgc ccgacaaccc gtgccgcaca
     1081 aggtggtcgc gggcgcggcg gtggtggagt cggcggtggc gcgggcggcg ctgccggcgg
     1141 cggcggggga tttgcccgcg gcggcggtgg gggacgcggg ggtggataca acttcaactg
     1201 gaataccccg ttcgtcctct cgcactttaa ctcacatatt ctctatgccg gcggcgatta
     1261 cgttttccgc tccgtcaatc gtggagatac gctcgatatc gcttctccga agatcatcct
     1321 cacgcagttc ggcagcgcga cggcgctgtc ggaatcgccg cgcaatgccg atgtcgttta
     1381 cgccggcacc gatgacggtg cgctctgggt gacgaccgac ggatgcaaga attggaagcg
     1441 cgtcgacgcc aacgtcggcc tgcccggacc gcgctgggtg ggggcaatcg aagcgtcgca
     1501 ctatgtcgat ggtcgctgct acgtcgtctt cgatgcccac cgctccaacg atgacaaccc
     1561 gtatgtctac gtcacggaag acttcggcaa aacctggaag ccgctgacgg ccaacctgcc
     1621 gtggggctcc acgcgctgca tcagcgaaga catcgtcaac ccgaacctgc tctacgtggg
     1681 caacgaattc ggcgcgtggt gctcgctgga tcgcggcaaa tactggaaca agctcggcgg
     1741 gaacctgccg acggtcgcgg tgttcgaatt cgcgcagcac ccggtgaacg ggcagatcgt
     1801 cgccgccaca cacggccgaa gtttgtgggt gctcgatgtc tcggcgttgc gccagatcaa
     1861 gcccgagtat ctggccgatg cgcccgagct ttatgaacct gcgcctgccg tacgctggca
     1921 cagcgagccg tcacgcggtg gaaccaatcg caagttcgtc ggccaaaacc ccgtcttcgg
     1981 cgtcgagatg ttctactccc ttccgcgcga cgcgaagaaa gccacgctga aggttctcga
     2041 cgtctccggg aaagcgctcc gcgaattccc cgcccgaagc gaagccgggc tgcatcgcat
     2101 cgcgtgggat ctgcgcctcg gcggcgcagg tggccgtggc ggatttggac gaggtggtgg
     2161 cttcggtgga ggcggcggcg gtggattcgg cgcagcagca gccggcgggg cagatgcttc
     2221 agcagctacc acaaccggcc gacgcggccg tggcggtcgc cgaggcggag cgacagccgc
     2281 tgcacccgac gccggcgaag cagccgcacc cggtgctgaa caaacccccg cagaacccgc
     2341 caacaccggc ggtgaaggcg gcggcgaatc gcaacccgtt gccgcccgcg gcggcggcgg
     2401 tcgtggattc gttggtgggg gcggcggtgg attcggtggc cgtggcggac ggggcggggg
     2461 atccgttcag gccggctcct atcgcatcgt cctcaccgtc gatggaaagg aattcgctca
     2521 gaacctccgc gtcgaaaatg accccgccgt cagcgactcg gtcgccatgg ccagcaactc
     2581 tcccgacttc ggtgaggaag aggaagacga agattcctac ttcaagatcg acggcgccga
     2641 cgaagatgcg gacatggatg ccgacgaaga gatgaacaat gccggcgaac aaagtaagcg
     2701 ggagcgcgac cgcgatgccg atcgtgatcg ggaacgcgac gccgacaagc tcgtcgcggt
     2761 tcggcagcag gtcactggct ttgcacagaa actgggaacc cgtaaatgaa gatagttcgc
     2821 atcgcgctgg gcgcgtcggt tgtagcggcg atgggcttat ccctcgccgc cgccgacccc
     2881 gcgccgcaga aaggtccgcg caaagacccg caagcgtcgt acgagccgcg ctccggcccc
     2941 ggcgaagggc agaaatatct ccagcgtttc gtcggcaagt ggaccgtcgt caaaacgttc
     3001 tacccgcgca acggagatcc ttccgtcgcg aagggtgatt gcgtgcagac gatgatcaac
     3061 gacggcaggt tcctgaaatc ggaattcacc ttgcacgccg aagccggcga caccaccggc
     3121 ttcggcctga tcggcttcga acccgaaacc ggcaagttca ccagcgtctg ggccgactcg
     3181 cgccagacgc gcatgtcctt ccgccagggc aatgaaccct tcgacggcaa ggaaattcac
     3241 ctggtcgccg cgacggtcga tccgagcgcc aagcctccgc gcccgtcgca cacggtcagc
     3301 cgaatctcgg atgatggaaa caccatcacc cacaagcagt tcgcctcagg cgccgatggg
     3361 aaggaccgcc tgatgatgga actggtgttg acgcgtaaag ccgaatgacc ggcaggtaac
     3421 tatttgtcct tgatctcggc aagcaaggac tttgcttccg tcgctgaagc ggttttggga
     3481 tagctctgga tcagcgcctg cagcttcgct cgagccgtgt catactttgc tgcgttgatg
     3541 tagctcttgg ccagccgcag ggcggctgag gccttggctt cgccgtcgtc gggcgtgggg
     3601 gtggcaggcg tcggagtagt cgacgtcgcc gccgagcccg catcaactgc cggcttcggt
     3661 gctgcagtcg ccggggtcgc agcaactgac ggcgcattgg cttctgtcgc gcctgcgtca
     3721 aggaactcaa cgccctgctc aaaccagctt gcagggtaat gcgtccaaag cgtcatgtcg
     3781 ctctgcggca ctagcagata cttcacgtgc ttgaaccccg cgcgtgtgta ctgctctccc
     3841 agtaactgca tcagatccgt gtgcgccggg tccgggcttt cgcggcccgc gaagaaattg
     3901 cgggtgcggg attttgccag agtcagctgc gcgggagtcg gttgattcag gattcccacg
     3961 ttgaaaaacc gccgggtgcg gatgtcggtg actttgtcga accagtagcc aaccgcactg
     4021 cacagctgcc cctggaaaat ctcgggataa taaagggcaa ttccttgcgc cagcgatttg
     4081 gtgccggcgg ggcagatata aacacgcgcc ggatcgatcg gccaggtttt ctcggcattg
     4141 tgaactgcat ccagcgccaa cccgatgctc gccgtcgggt tgatccctcc gttctgatct
     4201 ccgagccaga tcaggtgatg tttctccagc acatcgagcc aggcggctgg cgggaaccca
     4261 tggttgggca tgggcattgg caccatcaat ccatatttcc cgccgtcgcc ggccgctttg
     4321 gggacatatg cttcgaaaat ctctttggga agatcatagt ccggctccgg tgggccggct
     4381 ttttgcgagg tatgctttgc gatgtcagtc gcgttgctca gcggcgatcg ttctgtcagg
     4441 atcacctgaa aacggccggt gacgccctga accttgtcaa tcggcttcgg ctgctgcgcc
     4501 tttgcggcgc cgctcactcc aagaatcact gcaaaaaccc aactcagtat tttgcccggc
     4561 gtcaacatgg caatccctcc cttttcgcgg ttgataagaa gttcgttccc catccatcat
     4621 gtgcaaaaaa ggtcgatcag acaacccaat ttccagttac gacagctgcg gacctccggc
     4681 taccgcgcag gcgatgtgtg cccggtaacc gatctgctcg acgctgtaca acacgatcct
     4741 tgtccgatcg accttcgccc ggtcgccgaa cgggacaagg cccacatcgg gccgccagtt
     4801 accacgcgac gtggaacgcc atccaagcga agatcatctg ttcgcgatgc cgcctcaatg
     4861 tcagcccaat ggcaataggg gcggctccgt cgacggaaaa tctgcgggtc tcgacagccg
     4921 gcgcttccgg cgtataatcc cgccgcacat tcggtcccct aagcccaggt ggcggaattg
     4981 gcagacgcac tgctttgagg tggcagcgag taacatcgtg caggttcaaa tcctgtcctg
     5041 ggcattgaag ttacgcttcc cgctctctca ttttcccttc cagattcagg acgtgtccgc
     5101 tttcatgccc gcagttcgcg cattgcgctc tcgtgtgggg atatcattgt gcgaacacgg
     5161 tcgatgccct tgagcggcag gagcgtcaga tgacacgcac gattacagtt attgtgatgg
     5221 tcgttctcgc tgcactggcg atttcagtgc ggggcgacga agcgaaggag aaccccactg
     5281 tggataccac ggcgttgacc gtcgatttat atgcccaggt cggcaagcag
//
LOCUS       DASZMC010000634         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3816373,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000634 DASZMC010000000
VERSION     DASZMC010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3816373"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..603
                     /locus_tag="VG274_12870"
     CDS             97..603
                     /locus_tag="VG274_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07597.1"
                     /translation="MAQLPLADWRDFYVTIGTASGAIVGASFIVATLASGVKEPRIGL
                     RGFITPTAVHLGSVLVGSAILTVPTLDALSLAVLLGAGGLGGAVYGVVVATRIWSMNL
                     DLADRACYVVLPILAYGAIAAAALLERWAVTTALQTLAVSFVVILIIGMRNAWDMATF
                     LIARDREG"
     gene            623..853
                     /locus_tag="VG274_12875"
     CDS             623..853
                     /locus_tag="VG274_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07598.1"
                     /translation="MTPELILTWHDCFTTVAQASATLAGLLFVGVTISLNHLLQGRGY
                     LYRAWASISRPGWPGCSPSKSPRMELRKSAVP"
     gene            921..1619
                     /locus_tag="VG274_12880"
     CDS             921..1619
                     /locus_tag="VG274_12880"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWE07599.1"
                     /translation="MIEVRPFRTLGAANHGWLKATHHFSFAGYHDAGRMGWGALRVWN
                     DDEIAPKSGFPPHPHADMEIITYVRDGAITHEDSMGNTGRTGAGDVQVMSAGTGVRHA
                     EYNFENEPTRLFQIWIEPSQAGGKPSWGAKPFPKAERSGRFVTLASGLANDDDALPIR
                     AEARVLGATVKPGEHLTLALDPGRHAYLVPATGAIEVNGRRVNARDGAAISGEAGIEI
                     VGLEEAEIILVEAA"
     gene            1697..1918
                     /locus_tag="VG274_12885"
     CDS             1697..1918
                     /locus_tag="VG274_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07600.1"
                     /translation="MARIFSQRPLRILSVLGAKFCLLLWWNFVSSSVDRMEAAKAEWR
                     AGRMELPPGDDHEFIPLPDEPLPHPTDPV"
     gene            complement(1938..2954)
                     /locus_tag="VG274_12890"
     CDS             complement(1938..2954)
                     /locus_tag="VG274_12890"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWE07601.1"
                     /translation="MNSSRPEIRNLLRTAVAPFERPSLALGAYQIATSIGLYVATLAA
                     MYWSLGISYGLTLLLALPASALLVRVFIVQHDCGHGSFFGSQRANDILGAICGVLTLA
                     PYRNWRRQHSQHHANWNNLDRRDSGADIYSACLTVEEYRALPPWRRFFYRLPRHPVIA
                     HAMLPPLVFLLLYRIPFDTPNSWSRERRSVWGTNFAIAACVVALGLALGFRAVLLVQL
                     PIVVLATIAGVWLFSVQHRFDDAHWLRSREWNFHAAALNGSSYLKLPQPLQWMTGNIG
                     FHHIHHLAPRVPNYRLEACYRSNVDLQSGKPLTLPTAFRASTLTLWDEVRKKLVRFKD
                     AHAA"
     gene            complement(3005..3409)
                     /locus_tag="VG274_12895"
     CDS             complement(3005..3409)
                     /locus_tag="VG274_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07602.1"
                     /translation="MTCDALGEVYFERRAERAAEGPDRSRAELACLRDLIDVLLAHPA
                     GLRRWSVMRAIRTRREKAGEDLSLKFEDEVERAFRRQCADIQGKDGEKNRASAASALF
                     YRPKEKAGEVWAVHADRARVWLDAEVSAEAAR"
BASE COUNT          611 a         1120 c         1128 g          609 t
ORIGIN      
        1 cgcttccatc tggatggccc gcttgcgcgg gccatgacaa atcagggcgt agtgcgaatc
       61 tggaaatgct gtaggttgtc cccgggaggg gccgccatgg ctcagctgcc gcttgcggac
      121 tggcgcgatt tctacgtgac gatcggcacc gcgtcgggcg ccatcgtcgg cgcctccttc
      181 atcgttgcca cgctcgcctc aggcgtgaaa gagccccgga tcgggctgcg cggcttcatc
      241 acaccgaccg cggtgcatct gggcagcgtg ctcgtcggtt cggccattct caccgtgccc
      301 acgctcgatg cgctttcgct tgcggtattg ctgggtgccg gcggattggg aggcgcggtt
      361 tacggagtcg ttgtcgccac gcgaatctgg tcgatgaatc tcgatctcgc cgatcgcgcc
      421 tgctacgtgg tgctgccgat cctggcctat ggcgcgatcg cggcggcggc gctgctggag
      481 agatgggccg ttaccacggc gctgcaaacc ctcgccgtgt ccttcgtcgt gatcctgatc
      541 atcggcatgc gcaacgcctg ggacatggcg acctttctca ttgcgcgcga ccgggaaggg
      601 tgacggagaa tcgatgactc caatgacgcc cgaactcatt ctcacctggc acgactgttt
      661 cacgaccgtg gcgcaggcaa gcgccacgct tgcaggactt ctcttcgtcg gcgtcaccat
      721 cagcctcaat catctgctgc agggccgcgg ctacctctac cgcgcgtggg cctctatctc
      781 tcgaccgggc tggcctgggt gttcgccatc gaaatctccc cgcatggagc tgcggaagtc
      841 tgccgtgccg tgacggatgc tttcaaccgc aagatcgcac accatatacg tcccgaaccg
      901 ccattccggg gagtgcccgc atgatcgaag tcagaccgtt caggacactg ggcgccgcca
      961 accatggctg gctgaaggcg acgcaccatt tctccttcgc cgggtatcac gacgcagggc
     1021 gcatgggctg gggcgcgttg cgcgtctgga acgacgacga gatcgcgccg aaatcaggct
     1081 ttcctccgca tccgcacgcc gacatggaga tcatcaccta tgttcgcgac ggcgcgatca
     1141 cgcatgaaga ttccatgggc aacaccgggc gcacgggtgc cggcgacgtg caggtgatgt
     1201 cggccggcac tggcgttcgc catgccgaat acaatttcga gaacgagccc acgcgtctgt
     1261 tccagatctg gatcgagccc agccaggcgg gcggaaagcc gtcctggggc gcgaagccat
     1321 ttccgaaggc cgaacgttcc gggcgattcg tcacgctggc gagcggtttg gccaatgacg
     1381 acgatgcgct gccgatccgt gccgaagcac gcgtgctcgg cgccaccgtc aagccgggcg
     1441 agcatctgac gctggcgctc gatcccggcc gccacgccta tctggtgccg gcgaccggcg
     1501 cgatcgaagt gaacggcagg cgcgtcaacg cgcgcgacgg cgcggcgatc agcggcgagg
     1561 ccggcatcga gatcgtcggc ctcgaagaag ccgagatcat cctcgtcgaa gcggcgtaac
     1621 tgtagccaag caaaagattc ggcgccgagg acgctgagga tgcgcggagg gcgcaggggg
     1681 aagacatcgc gcgcctttgg cgcgcatttt ttctcagcgt cccctgcgca tcctcagcgt
     1741 cctcggcgct aaattctgtc ttcttctctg gtggaatttt gtctcgtcga gtgtggatcg
     1801 catggaagcc gcgaaggcgg agtggcgcgc gggccgcatg gagctgccgc ccggcgacga
     1861 ccatgaattc attccgctgc ccgacgagcc attgccgcac ccgacggacc ccgtgtgatt
     1921 ggcatcatgc aagttgttca ggccgcgtgt gcgtccttga agcgcaccag cttcttgcgg
     1981 acctcgtccc acagcgtcag cgtcgaggcg cggaaggctg tgggcagggt caggggtttc
     2041 ccgctctgca gatccacgtt gctgcgataa cacgcctcca gccgatagtt cggcacgcgc
     2101 ggcgccagat gatgaatgtg gtggaagccg atattcccgg tcatccattg caggggctgc
     2161 ggcagcttca gataggacga gccgttcagc gccgccgcgt gaaaattcca ttccctcgag
     2221 cgcagccaat gcgcatcgtc gaaacggtgc tgcaccgaaa acagccagac gccggcgatc
     2281 gtcgccagga ctacgatcgg cagttgcacg agcaggacgg cgcggaagcc cagcgcgagc
     2341 cccagcgcca cgacacaggc ggcaatcgcg aagttggttc cccacaccga ccggcgctcg
     2401 cggctccagc tgttcggcgt gtcgaacggg attcgataga gcaggagaaa caccagcggc
     2461 ggcagcatcg catgagcaat cacgggatgg cgaggcagcc ggtagaagaa tcgccgccag
     2521 ggcggcagcg cgcgatattc ctccaccgtg agacaggccg agtagatgtc ggcgccggaa
     2581 tcgcgccggt cgagattgtt ccagttcgcg tgatgctgcg aatgctggcg gcgccagttc
     2641 cgatagggcg caagcgtcag cacgccgcag atagcgccga gaatgtcgtt ggcgcgctgc
     2701 gacccgaaga acgatccgtg cccgcaatca tgctgcacga tgaacacccg tacgagaagc
     2761 gcgctggccg gcaaggcgag cagcagcgtc agcccgtacg agatgccgag cgaccagtac
     2821 attgccgcga gcgtcgcaac atagagtccg atcgaggttg caatctggta ggcgccgagc
     2881 gcgaggcttg gcctttcgaa gggtgcaact gctgtccgca gaaggttgcg gatttcgggc
     2941 cgggaagagt tcatgccgtc tccgcgcgag agaccggaat cgttcttcgg gcgccgggaa
     3001 cggctcagcg agccgcttcg gcggacactt ctgcgtcgag ccataccctg gccctgtccg
     3061 catggacggc ccagacttcg ccggccttct ccttcgggcg gtagaacaaa gcgctcgccg
     3121 cgctggcgcg gttcttctcg ccatccttgc cctgaatatc cgcgcactgt ctgcggaagg
     3181 cgcgctccac ctcgtcctcg aatttcaggg acaggtcttc acccgccttc tcgcggcgcg
     3241 tgcggatcgc acgcatgacg gaccagcgcc gcaatcctgc cggatgggcg agaagcacgt
     3301 cgatgagatc gcgcaggcag gcgagctcgg cccgggaccg atccggtccc tccgccgcgc
     3361 gctcggcccg gcgctcgaaa tagacttccc ccagagcatc acaggtcatt ttgcatccat
     3421 tggtgcgttt cggggcggcg tcggataacc accgccgccc cgcgggac
//
LOCUS       DASZMC010000635         7584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1900324,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000635 DASZMC010000000
VERSION     DASZMC010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900324"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VG274_12900"
     CDS             <1..192
                     /locus_tag="VG274_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07603.1"
                     /translation="VGVAFELAERMLRRQCGDPTEAAAFATALFLGGVRGLPHLHSAL
                     GNGNALPQETIQDGNGSVS"
     gene            complement(165..1058)
                     /locus_tag="VG274_12905"
     CDS             complement(165..1058)
                     /locus_tag="VG274_12905"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07604.1"
                     /translation="MSRQRRPPRAERAPRTVERGRPQRAVIKGQDRSRWTDVYHGVLT
                     APWWAFFAGTAFIFALVNTVFALLYRADPKGIDHARGFWDYFLFSVQTIGSANYTVML
                     PKSIYANVVVSVEAFFGILNLALVTGVVFARFSRPFARVVFSKVALIAPFDGVPTLMF
                     RAANQRANQILDAAVTVSLARQMGTKEGIVMRRFEELTLVRARTPLFALSWTVMHVID
                     EASPLHGVTSESLYDSQIEIIVLLRGTDDTLADIIYARHSYMPDEILWDRRFVDVLAL
                     TPSGRRIVNLHRFHDTEPLPS"
     gene            complement(1116..1487)
                     /locus_tag="VG274_12910"
     CDS             complement(1116..1487)
                     /locus_tag="VG274_12910"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWE07605.1"
                     /translation="MNGEAARWPTELRVDHGRATLTVTFDSGERFALPAEYLRVESPS
                     AEVQGHSAAQKQLVPGKRHVRIESLEPVGNYATRIIFDDGHDTGLYSWDYLHELGSEQ
                     NTRWAAYLSELRRRSASRERG"
     gene            complement(1515..1709)
                     /locus_tag="VG274_12915"
     CDS             complement(1515..1709)
                     /locus_tag="VG274_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HWE07606.1"
                     /translation="MIEPDPRLLEILVCPLTKTTLIYDRERGELISRAAGLAYPIRNG
                     IPIMLPAEARELSDAERNAK"
     gene            complement(1717..2367)
                     /locus_tag="VG274_12920"
     CDS             complement(1717..2367)
                     /locus_tag="VG274_12920"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWE07607.1"
                     /translation="MRTYTSLDELPATMPVFPLTGAVLLPRGQLPLNVFEPRYLAMAD
                     AALSGARFIGMIQPTEHEEKVLTPPLSKVGCAGRITSYKETDDGRCLITLTGICRFRV
                     AEELAVKTTFRQIRPDYSAYVGDLVADSTSDFPRERLIGALKDYLAQRDLKADWRSVM
                     SAPPETLVNALAMLCPFGPAEKQALIEANSWNDRVDTLVTLLEMSASPAPGNASLH"
     gene            complement(2373..3320)
                     /gene="trxA"
                     /locus_tag="VG274_12925"
     CDS             complement(2373..3320)
                     /gene="trxA"
                     /locus_tag="VG274_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWE07608.1"
                     /translation="MALIGPSGRPLTGSTPKTAPGAASPGALVKDGSIDTFAADVLDA
                     SKQVPVIVDFWAPWCGPCKQLGPALEKVVNEAKGAVRLVKINIDENQEIARQLRIQSI
                     PTVFAFRDGQPVDGFMGAVPESQLRAFVQQLTGGAAGHDQAENILASADEAYTAGDVA
                     GAAQAYGHVLQDEPGHPRAVAGLARCYLKSGDVERARKTLGLVRPDGASDEAIRIVDA
                     ELKLREQAAKAGDSAPLRARLDAEPNNHQARLDLALALDARGDREGAIAELLELVRRD
                     RKWNDEAARKHLVTFFEAMGPTDPRTLAARRQLSSILFS"
     gene            complement(3433..5316)
                     /locus_tag="VG274_12930"
     CDS             complement(3433..5316)
                     /locus_tag="VG274_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HWE07609.1"
                     /translation="MAKPHPLLESGDGTPSLRPLYMLLPHLWPRGRPELKLRVAASMV
                     CLLLAIAATGISPLLLGWVTDQLSRSMPSSIAMAATLGLIVAYALSRVVMQLFAQLRD
                     GVFAKVLYHALREVGVAAFAHVHTLSLRFHLERKTGGLSRVIERGTKGIDTLLSFATF
                     SIFPTLLQVLIFTVEIIWKLNVWIAVTALAMVAGYVWFTFAITRWRIQFRRDMNQSDT
                     DANTKAVDSLLNYETVKYFNNEAHETRRFDRSMGRFARASVQAQISLSVLNTGQAAIM
                     ALGTGIVMVLTTIGIRQGHFTVGDFVMANAILMQLYVPLNLLGTVYREITQALVDMES
                     MFRLLYQPQEVSDKPGARDLAVGGGDIRFDNVVFAYDPERVVLKGISFRVPAGKTVAV
                     VGPSGAGKSTISRILYRFYDIASGSVKIDGQDIRDVTQASLRAAIGIVPQDTVLFNDT
                     IRYNIAYGRIGANEAEIKEAAQVAQIDPFIRELPLGYASMVGERGLKLSGGEKQRVAI
                     ARTVLKNPPILLLDEATSALDTHTEREIQSALAAVTRNRTTLVIAHRLSTVVEADEIL
                     VLDHGVIVERGRHSELLAKGGQYAEMWNRQKEAAAARERLKAVESDPDVEPQFTRESV
                     AAD"
     gene            5428..6078
                     /locus_tag="VG274_12935"
     CDS             5428..6078
                     /locus_tag="VG274_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWE07610.1"
                     /translation="MAHRNTGGHNPVAESAPGQTAPEPADSHRGYQPAICVFCGSSLG
                     NDLLYAKTARDLGRQIGEGGYRLVFGGGAVGLMGEVARTARSAGAPIIGILPAFLRGV
                     EPPLKSAEELVITPDLQQRKSRMLALADAFVILPGGLGTLDEFFEVVTSTQLKVHSKP
                     VVVVDVAGYFAPLRALLDQVVAHGFARPEIANHQEFVATPAEALARITLRLSARGR"
     gene            complement(6047..6961)
                     /gene="rarD"
                     /locus_tag="VG274_12940"
     CDS             complement(6047..6961)
                     /gene="rarD"
                     /locus_tag="VG274_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HWE07611.1"
                     /translation="MSDRTRPDEARGIAYAAIAYVLWGAVPLYWRHFDGVAPFEVIVH
                     RIVWCALFVMAITVARGRIPHVAAIFRNPRLLATLALTSVLITCNWSVYIYSVATRQL
                     VEASLGYYITPLISIGLGVVLFGEHLSRLRLLAVCLAGVAVAVKAASLGHIPMIASVL
                     ALSFGFYGYFRKQAPVDALDGLLVETGILLPAAAGLLVFWYMIDNASFPSGSIGRDSL
                     LMASGPVTAIPLALFAAGARRIRLSTLGFLQYLAPSLTLLLAVFGFGERFTRLDAVSF
                     GCVWGALVLVALDGRLSRFSARAPTIGV"
     gene            complement(6972..>7584)
                     /locus_tag="VG274_12945"
     CDS             complement(6972..>7584)
                     /locus_tag="VG274_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWE07612.1"
                     /translation="PHHENEIAQSESAHAGRALARVWLHNGFLNAEGGKMSKSLGNFV
                     TIRELLADWPGDVLRLNMLRTHYRQPIDWTVRGMNESWTILERWYEGAEPVASRRMGE
                     VFLDALLDDLNTPQAIAELHKAEPEELAGGLVLLGFSPDPGRIAARSPIPVEEIAHAI
                     AARTSARKARNFPEADRIREELAAKGILLEDNQDGTTSWEVKR"
BASE COUNT         1474 a         2399 c         2337 g         1374 t
ORIGIN      
        1 gtcggtgtcg ccttcgagtt ggcggaacgc atgctccgtc gtcagtgtgg cgatcccacc
       61 gaagccgccg ctttcgcgac cgcgcttttt cttggcggcg tgcgcggcct gcctcatctg
      121 cattctgcac tgggcaatgg caacgccttg ccgcaagaaa ccattcagga cggcaatggc
      181 tcggtatcgt gaaaccggtg aagattgacg atccggcgtc ccgagggcgt cagcgccagc
      241 acatccacga aacgccgatc ccagagtatt tcgtccggca tgtaggaatg ccgcgcatag
      301 ataatatcgg ctaaagtgtc gtcggtgccg cgcaagagga caatgatctc gatctgcgaa
      361 tcgtagagcg attcggatgt gacaccatgg agcgggctcg cttcgtcgat cacatgcatc
      421 acggtccacg acagcgcgaa gaggggggtg cgcgcgcgga cgagagtcaa ttcctcgaag
      481 cggcgcatca cgatgccctc tttcgtcccc atctgccgtg ccagcgagac ggtgacggcg
      541 gcatccaaaa tctgattggc gcgctggttg gcggcgcgga acatcaacgt gggcacgccg
      601 tcgaacggtg caatcagcgc aaccttgctg aagacgacgc gcgcgaacgg ccgggagaat
      661 cgtgcgaaca cgacacccgt gaccagcgca agattaagaa tgccgaagaa cgcttccaca
      721 gacacgacga cgttcgcgta gatgctcttc ggcagcatca ccgtgtagtt ggccgatccg
      781 attgtctgca cgctgaacag aaaatagtcc cagaaaccgc gggcatgatc gatgcccttg
      841 gggtcggccc tgtaaaggag tgcaaagacc gtgttgacga gggcaaagat gaacgcggta
      901 ccggcaaaaa atgcccacca cggcgccgta agcacgccgt gatagacgtc cgtccagcgt
      961 gagcggtcct gacctttgat gaccgcccgc tgcggccggc cgcgctcgac cgtgcgcggc
     1021 gcacgctccg cccgaggcgg ccgcctctgc cggctcatgg agctcgggtc ggtgcgagct
     1081 gtatgcgcgt catttccgcc attcccccga agggttcagc cgcgctctcg cgacgcgctc
     1141 ctccggcgca gttcggaaag ataggcggcc catctggtat tctgctcgct cccaagctcg
     1201 tgcagataat cccagctgta aaggccggtg tcgtgtccgt cgtcgaagat gatgcgcgtt
     1261 gcgtaattgc cgaccggctc gagcgactcg atccgcacgt ggcgtttgcc cggcaccagc
     1321 tgcttctgcg cagcagaatg tccctgcact tcggcgctcg gcgactcgac gcgcagatat
     1381 tctgcaggaa gcgcaaagcg ctcgccgctg tcgaacgtca ctgtcagcgt cgcccgcccg
     1441 tgatcgacac gcagctcggt cggccatcgc gcggcctccc cgttcacgca ccactcctca
     1501 tccgccgccg ccggtcattt cgcgttcctt tccgcgtcgg acaattcgcg cgcttcggcc
     1561 ggaagcatga tcggaatccc gtttcgtatc gggtaggcga gcccggccgc ccggctgatc
     1621 agttcgccac gctcccggtc atagatcaat gtcgtcttgg tcagcggaca gacgagaatc
     1681 tccagcagcc ggggatctgg ttcgatcatt gcgcactcaa tgcagcgacg cgttgccggg
     1741 cgcaggtgac gccgacattt cgagcagggt cacgagcgtg tcgacgcgat cgttccagct
     1801 gttggcttcg atcagggcct gcttttcggc cggcccgaat ggacaaagca tcgcgagagc
     1861 gttcacaagc gtttccgggg gcgcgctcat taccgaacgc caatcggctt tcaggtcgcg
     1921 ctgtgcgagg taatccttga gcgcgccgat cagtctttcg cgaggaaagt cagacgtcga
     1981 atccgccacc agatcgccga cataggctga atagtcggga cggatttgcc gaaacgtcgt
     2041 tttgacggca agttcttcgg caacgcggaa acggcaaatc ccggtcaagg ttataaggca
     2101 acgcccgtcg tcggtttcct tgtagctcgt aatcctgccg gcacagccga ccttcgaaag
     2161 cggcggagtc agaactttct cctcgtgttc ggtcggctgg atcatcccga tgaaccgggc
     2221 gcccgacagc gctgcatcgg ccatcgccag atagcgcggt tcaaaaacgt tgagcggcaa
     2281 ttgcccgcgt ggcaacagaa cggctcccgt caaagggaac accggcatgg tcgccggcag
     2341 ttcatcgaga cttgtatagg tccgcatgag tctcaggaaa aaaggattga cgacaattgg
     2401 cgccgtgccg cgagcgtccg cggatcggtc ggccccatcg cctcgaagaa cgtgaccaga
     2461 tgcttgcgcg cagcctcatc gttccatttg cgatcccgcc ggacgagctc cagaagttct
     2521 gcgatcgcgc cctcgcggtc gcccctggca tcaagcgcca gtgccagatc caggcgcgcc
     2581 tggtggttgt tcggttccgc atcgaggcgc gcacgaagcg gcgcgctgtc gccggccttg
     2641 gcggcctgtt cgcgcaattt gagttctgcg tcgacgatgc ggatcgcttc atcggaggcg
     2701 ccgtcaggcc gaacgagccc cagcgtcttg cgcgcgcgtt cgacatctcc gcttttcaga
     2761 taacagcgcg caaggccggc gacggcgcgg ggatgcccgg gctcatcctg cagcacgtgg
     2821 ccataggcct gtgccgcccc ggcgacgtcc cccgccgtat aggcttcatc cgccgacgcg
     2881 agaatgttct cggcctgatc gtggccggcc gcgccaccgg tcaattgctg cacgaaggca
     2941 cgcagctgcg attccggcac cgcgcccatg aagccatcca ccggctgccc gtcgcggaat
     3001 gcaaagactg tcggaatcga ctggatacga agctgtcgcg cgatttcctg gttctcgtcg
     3061 atgttgatct tgacgagccg caccgcacct tttgcctcgt tcacaacctt ttcgagcgcg
     3121 ggcccaagtt gcttgcacgg gccgcaccat ggcgcccaga aatcgacgat gaccgggacc
     3181 tgcttggacg catcgagcac gtcggcggcg aaggtgtcga tcgagccgtc cttgacgagc
     3241 gcgcccggcg aggctgcgcc tggcgccgtc ttcggtgtac tccccgtaag cggcctgccg
     3301 ctgggaccta tcaatgccat tcaaaaaact ctctcaagcc cgcgaagcaa agatgggcac
     3361 attgggtcgg aaagaaaaga gcgaaagcgc accgggcggc cttttcgacg gtccaagggg
     3421 ttcgaacgac gatcagtccg cagcaacgga ctcgcgcgtg aattggggtt caacatcggg
     3481 atcgctttcg acggccttga gccgttcgcg cgccgccgca gcctccttct gcctgttcca
     3541 catctcggcg tactgaccgc ctttcgccaa cagttccgag tgtcgcccac gttccacaat
     3601 cacgccgtga tcaagaacca gaatctcgtc cgcttcgacg accgtggaca gccggtgcgc
     3661 gataacgagc gtcgtgcgat tgcgcgtgac agccgccagt gcgctctgta tctcgcgctc
     3721 cgtgtgggtg tcgagtgcgg acgtcgcctc gtcgaggagc agaatgggcg gattcttgag
     3781 cacggtgcgg gcgatcgcta cccgctgctt ctcgcccccc gagagcttca atccacgctc
     3841 gcccaccatg gaggcatagc cgagcggcaa ttcgcggatg aacggatcga tctgcgcaac
     3901 ctgcgccgcc tccttgatct cggcctcgtt cgcgccgatc cggccatagg cgatgttgta
     3961 gcgaatcgtg tcgttgaaga gcacggtgtc ctgcggaacg atgccgatcg ccgcgcgcaa
     4021 tgaagcctgg gttacgtcgc ggatgtcctg gccgtcgatc ttcacgcttc cgctggcgat
     4081 atcgtagaaa cggtaaagaa tgcgcgaaat ggtcgacttg ccggcgcccg agggaccgac
     4141 caccgcgacc gtcttgccgg cgggcacccg gaaggagatt cccttcagca ccacccgctc
     4201 gggatcataa gcgaacacaa cgttgtcgaa acggatatcg ccgcctccta ccgcgagatc
     4261 cctcgcgccg ggtttgtcgc tcacctcctg tggctgatag agcaggcgga acatggattc
     4321 catgtcgacg agcgcctggg tgatctcccg atagacggtg ccaagaagat tgaggggaac
     4381 atagagctgc atcaggatgg cgttggccat cacgaaatcg cctaccgtga aatgtccctg
     4441 gcggatgccg attgtcgtca gcaccatcac gatgcctgtg ccgagcgcca tgatcgcggc
     4501 ctggccggtg ttgaggacgg acaatgaaat ctgcgcctgc accgaggcgc gcgcgaaccg
     4561 tcccatggag cgatcgaagc gccgcgtctc atgcgcctcg ttgttgaagt atttcaccgt
     4621 ctcgtaattg agcaggctgt cgaccgcctt ggtgttggca tcggtatcgc tctggttcat
     4681 gtcgcggcga aactggatgc gccatcgggt gatcgcaaag gtgaaccaca catagcccgc
     4741 gaccatggcg agcgccgtca ctgcaatcca gacgttcagc ttccagatga tttcgaccgt
     4801 gaagatgagc acctgcagga gcgtgggaaa aatgctgaat gtcgcgaatg aaagaagcgt
     4861 atcaatgcct ttcgtgccgc gctcgatgac gcgggacagc ccgcccgtct ttcgctccag
     4921 atgaaagcgc aggctgagcg tgtgcacatg ggcgaaggcg gcgacgccca cctcgcgcag
     4981 cgcatgatag agcactttgg cgaacactcc gtcgcgcaat tgggcgaaga gctgcatcac
     5041 gacgcgcgac aaggcatagg cgacgatcaa gccaagcgtc gccgccatgg cgatggaaga
     5101 aggcatgctg cgcgaaagct ggtcggtcac ccagccgagc agaagcggcg aaatacccgt
     5161 ggccgcgata gcgagcagaa ggcacaccat cgatgccgca acgcgcaatt taagttcagg
     5221 gcgccccctg ggccagagat ggggcaacag catgtaaagc ggccgcaacg agggcgtgcc
     5281 gtcgccggat tcaagcaagg gatgcggctt tgccatgctg ccgttttatc ctatacccgc
     5341 cgccgtatcc gcctctgccg cgtttacgtg aatgagcggc gcaggcgggt ttaatcactt
     5401 tgcgcatcag actgtttagg tatccgaatg gcgcaccgca ataccggcgg acacaaccct
     5461 gtcgcagaat cggcgcccgg acagaccgcg ccggaaccgg cggattcaca tcgcggctac
     5521 cagcccgcaa tctgcgtatt ctgcggatcc tcattgggca acgacctcct ttatgcgaaa
     5581 acggcgcgcg acctcggccg ccagatcggc gagggcggct acaggctcgt tttcggcggc
     5641 ggtgctgttg gcctgatggg cgaagtggcg cgcaccgcgc gaagcgcggg ggcgccgatc
     5701 atcggcattt tgccggcgtt ccttcgtggc gttgagccgc cgctcaaatc cgcagaagaa
     5761 cttgtcatca caccggatct ccagcagcgc aaatctcgca tgctcgcgct cgccgatgcg
     5821 ttcgtgattc tgcccggcgg cctcggcacg ctcgacgaat tttttgaagt cgtcaccagc
     5881 acgcagctca aggtgcattc caagcccgtc gtcgtggtcg acgtcgccgg atatttcgcg
     5941 ccgctccggg cgctcctcga tcaagtcgtc gcgcatggat tcgcgcggcc cgaaatcgcg
     6001 aaccaccagg aatttgtcgc gacgcccgcg gaggccttgg cgcgaatcac actccgattg
     6061 tcggcgcgcg ggcgctgaag cgcgacaggc gtccatccag agccaccagc acaagcgcgc
     6121 cccatacgca accgaatgat actgcatcga gccgcgtgaa cctctcgccg aagccgaaga
     6181 ccgcaagcaa aagcgtcagg gagggcgcca gatattgcag gaagccgagt gtggaaagcc
     6241 gtatgcgccg cgcccctgcc gcaaaaagcg ccagcggtat tgcggtgacg ggtccgcttg
     6301 ccatcagaag cgagtcgcgg ccgatcgagc cggaaggaaa cgacgcattg tcgatcatgt
     6361 accaaaagac gaggagcccc gccgcggccg gcaggagaat gccggtctcc acgagcagac
     6421 cgtcgagtgc gtccactggc gcctgcttgc ggaaatagcc atagaagcca aacgacaacg
     6481 cgagcaccga cgcgatcatg ggtatgtggc caagcgaggc tgccttgacc gccacggcga
     6541 caccggcgag acataccgcc agaagacgaa gccgggacag gtgttcgccg aaaagcacca
     6601 cgcccagccc gatcgagatc agtggagtta tgtaatagcc gagactcgcc tcgacgagtt
     6661 gccgtgttgc aaccgagtat atgtagaccg accagttgca tgtgatcagc acgctggtca
     6721 gcgcaagcgt agccagcagc cgcgggtttc ggaatattgc cgcgacgtgg ggaattcgtc
     6781 cgcgcgcgac ggtaatcgcc atcacgaaga gcgcacacca aacgattcga tggacgatca
     6841 cttcgaacgg cgctacgccg tcgaagtgcc gccagtagag tggcaccgcg ccccacagca
     6901 cataggcgat cgcggcgtaa gcaattccac gcgcttcgtc gggccgcgtg cggtcgctca
     6961 tggcagccgc ttcaacgctt tacttcccaa ctcgtcgtgc cgtcctgatt gtcttcgagg
     7021 aggatgccct tcgccgccag ctcctcgcgg atacgatcgg cttccgggaa attgcgtgct
     7081 ttgcgagcag aggtgcgggc cgcgatggcg tgcgcgattt cctcaacggg tatgggagac
     7141 cgcgcagcaa tccgcccagg atccggagag aaccccaaca gcaccaaccc gccggcaagt
     7201 tcctccggtt cggctttgtg caattccgcg atcgcctggg gcgtgttcag atcgtcgagc
     7261 agcgcgtcga ggaaaacttc gcccatcctg cgtgacgcaa ccggctccgc gccttcgtac
     7321 cagcgttcga ggatggtcca gctttcgttc atcccccgca ccgtccagtc gatgggctgg
     7381 cggtaatgcg tgcgcagcat gttgagccga aggacgtcgc cgggccaatc ggcaagaagc
     7441 tcgcggatgg tcacgaaatt cccgagcgac ttcgacattt tcccgccttc ggcattcagg
     7501 aagccgttgt gcagccagac ccgcgccagt gcgcggccgg cgtgagcgct ttcgctctgt
     7561 gcgatctcgt tctcatggtg tggg
//
LOCUS       DASZMC010000636         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2468930,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000636 DASZMC010000000
VERSION     DASZMC010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468930"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VG274_12950"
     CDS             complement(<1..1055)
                     /locus_tag="VG274_12950"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWE07613.1"
                     /translation="MIHRPKKSQTKDQAVAVVFRLFLGGLVIIRLLTCLSASDWEEIR
                     KGPPPQEVLAKLDALTATLDSTELCRRAYDLTHDPDYLFACQRNARTVGERIDALRVL
                     LNSNAGLVHAVDLLDPAIRNWMDALCRPDKPRQARPRDVAKIVNIIHDSQTQSFPSDT
                     RVRAEQAFSGRLVALALTLVELAVLVLANWAFHRFMGRRRSTEEVLRRKEEFARSTVD
                     ALATHIAILDGNGIVLAVNRAWREYRETNGADMERVGEGVNYLIVCDAAGGQHCAEAA
                     AFGNGIRAVLAGKQEEFSIEYASHSRQERRWFVGRVTRFPGAEGSVEKRYVPRLVISH
                     DNITARKLAEEALQKAK"
     gene            1391..2422
                     /locus_tag="VG274_12955"
     CDS             1391..2422
                     /locus_tag="VG274_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWE07614.1"
                     /translation="MDTVRVGIIGFGRIGAEHAGWLSSAGNARAVAVVDATPARQELA
                     RQRGLCVVDALDALLTDSSIDAILVSSPTAMHFEHASRALAAGKHLMVEKPMALDLHQ
                     ARTLRNEAKTHKLVLSVFHNRRWDIDYLTVRDAIASGAFGKVINIESRLGQFSSCVGP
                     AAKEYRPNWRNEASFGGGGLYDWGSHFVDQIWRLLLPARPLRVFAQLRANLWSQDCDD
                     FARVLIDFDDGVAGLVEINTTTMAPLPRWQIDGTQGSASSPASLAFDTRAWADLQFTP
                     AAGEPRAFPIAAQGLSETQIWDQFASSVRGQGEPAVTADSVLPTMELLDAARESSRTG
                     RAVEVNPET"
BASE COUNT          458 a          787 c          758 g          468 t
ORIGIN      
        1 tccttggcct tctgcaaggc ttcttcggcg agcttgcggg cggtgatgtt gtcgtgcgag
       61 atgaccagtc ttggaacgta gcgtttttcc acggagcctt ccgctcccgg aaagcgcgtc
      121 acgcggccga caaaccaccg gcgctcttga cgcgagtgcg acgcatattc gatcgaaaac
      181 tcttcctgtt ttcccgccag cacggcgcga attccgttcc cgaacgccgc cgcctcggca
      241 caatgttgcc ccccggcggc gtcgcagacg atcaggtagt tcacgccttc gccgacgcgt
      301 tccatgtcgg cgccgttggt ctcgcggtat tcgcgccagg cgcgattgac ggccaggaca
      361 attccattgc cgtcaaggat cgcgatatgg gtggccaggg catcgactgt cgaacgcgcg
      421 aactcctcct tgcgccgcag cacctcctcg gtgcttcgcc gacggcccat gaagcgatga
      481 aacgcccagt tcgccaggac cagcaccgcc agttccacca gcgtgagcgc cagggcgacg
      541 agcctgccgg aaaatgcctg ttccgcccgc acccgggtgt cggaggggaa actctgagtc
      601 tgcgagtcat ggatgatgtt gacaattttc gccacatcgc gggggcgggc ctggcgcggc
      661 ttgtcggggc ggcacagcgc gtccatccag tttcgaatcg cgggatcgag caggtccacc
      721 gcatgaacca atcccgcgtt cgaattcaac agcacgcgca gcgcgtcgat ccgctcgcca
      781 accgtgcgcg cattgcgctg acatgcgaaa agataatccg gatcgtgcgt gaggtcgtaa
      841 gcccggcggc aaagttcggt tgaatcgagc gtcgcggtca aggcgtcaag cttcgcgagc
      901 acttcctgcg gcggggggcc tttgcggatt tcttcccagt ccgaggcgct gaggcaggtc
      961 aacagccgaa tgatgacaag gccgccaaga aacagccgga atacgactgc aaccgcctga
     1021 tctttcgttt gcgacttctt tggcctatga atcacatcgc tcccatttgc ccgccgacgg
     1081 gcttccatcc tcgtatcgga tgggatgagg gcggagttaa tgtggggagc ctgagtcggt
     1141 gtaatcggtc cgatattatg tggcatgggc agcctgccca tgaagtcgcg gtacgccgcg
     1201 acgttcgacg gtactccgat cgaagaagca agagcgaagc gaacttatta ggagaatcgg
     1261 tcgctgcgct cctgcgtttt ttggctggag taccagccaa cgcgacggcg taccgtcgct
     1321 cacgggcggg ccgcctgtgc cacgtggccc atgggttccc cgttgaatcg cgctccccta
     1381 caatctccgc atggataccg tccgcgtagg aattattgga ttcggtcgca tcggcgctga
     1441 acatgccggt tggctctcat cagcaggtaa tgcccgcgcg gtggctgtgg tagacgccac
     1501 gcccgcgcgc caggaactcg cccggcagcg cggtctttgc gtcgtcgatg ctctcgatgc
     1561 gctcctcact gactcttcca tcgacgcgat tcttgtctcc tcgcccacgg ccatgcactt
     1621 cgagcacgct tcgcgggcgc tggcggcagg aaagcatctg atggtggaaa aaccgatggc
     1681 ccttgatctg catcaggcgc ggacgctgcg caatgaagcg aagactcaca agcttgtgct
     1741 cagcgtgttt cacaatcgcc ggtgggacat cgactacctg accgtccgcg acgccatcgc
     1801 ttccggcgcc ttcggaaagg tgatcaacat cgaaagccgg ctcggccaat tctccagttg
     1861 cgtcggcccg gcggcaaagg aataccgccc caactggcgc aacgaagcca gctttggcgg
     1921 cggcggactt tatgactggg gcagccattt cgtcgatcag atctggcgcc ttttgcttcc
     1981 ggcgcgcccg ctgcgcgtct tcgcccagct ccgcgccaat ctctggagcc aggattgcga
     2041 cgacttcgcc cgcgtcctga tcgacttcga cgacggggtc gccggcctcg tcgaaatcaa
     2101 caccaccacc atggcgccgc ttccccgctg gcagatcgac ggaacgcaag gatcggcatc
     2161 gagccccgcg agcctcgcgt tcgacacgcg ggcatgggcc gatctgcaat tcactcccgc
     2221 cgcgggcgag ccgcgagcat ttcccattgc ggcacaggga ttgagcgaga cgcagatctg
     2281 ggatcaattc gcctcatccg tccgcggtca aggcgagccc gccgtcacgg ccgacagcgt
     2341 gctgccgacc atggagttgc tcgatgcggc aagagaaagc agccggacgg ggagagcggt
     2401 tgaggtgaat cccgaaacgt aattcaatga agatcgattt caccacggag gcacggagac
     2461 acggaggcgc a
//
LOCUS       DASZMC010000637         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2183346,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000637 DASZMC010000000
VERSION     DASZMC010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183346"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VG274_12960"
     CDS             <1..960
                     /locus_tag="VG274_12960"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWE07615.1"
                     /translation="ASFASLGCGAHHFGSNGPVDAAPAFPYHLPMPQSNPDCILAPLS
                     ALQWLVEAGADEAVGDEPVNRFDHASVRSRSEPFETTASPLALRPSAARPRNVPARPV
                     APTSSEGDQIGHAMEIAAACGTLAELKAALEAFEGCPLKKHATRTVFADGTPDRRILF
                     IGEAPGKEEDIAGLPFVGRAGKLLDRMLASIGLDRKSNVYITNILNWRPPSNRDPTPE
                     EAAICLPFLRRHIELVAPRIIVLLGAIASRHVMGRSEGIMRLRGSWLEYFVCGSMVPV
                     MPTLHPAYLLRRPPEKKLAWRDLQAISVKIQALALLERNADSG"
     gene            1002..2813
                     /locus_tag="VG274_12965"
     CDS             1002..2813
                     /locus_tag="VG274_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWE07616.1"
                     /translation="MARNIFLVASCVFCLAAPAAVGADSDPVSPQETASTAPHVLSLE
                     DARHYREIFADELDGRFAEARALIADLSDRSLIGYVEAEHYLSPYSRHAAIGDLAQWL
                     ATYAELPVAERIRALAELRNHHRRHKILLAGLPGIPRRATGGYDVTDSLANASLASDA
                     ARSALVQIDADVKLDQPAAAEIVLQQLAASNIAPPSDITRLAQRLAASYLAEGQDDAA
                     LRIAGSIAGVERESAPLLDWDAGLAAYRLNKFADAAPYFEHLAQSGAVPGWTRSAAAF
                     WAARSHVASGDPLPVVSLLSAAAREQPTFYGMLAQRLLGQEPSAGFSDPVLDSARFDE
                     MMQIPAAHRAVALWQTGHTEGVEQEMSRAFAEIDLRDADAFAALSHRLDLPALELRAS
                     EAQAARGELLSGLFPVPGYTPPGGYHIDRCLLLAFARVESRFQPRAVSMAGARGVMQV
                     MPGTAQQIDGATPARSQLNDPAYNLGLGERYLQQLLDRTNGNLFQLAAAYNAGPGALM
                     RWLGTRPEITNDPLLFIESLPYAETRNYIKHVMTYYWMYARRTNETAPSLQETAEGKW
                     PLYRSNAALPVAVPAPSGTPQPATAATLLVSDASAPH"
     gene            2794..3348
                     /gene="moaB"
                     /locus_tag="VG274_12970"
     CDS             2794..3348
                     /gene="moaB"
                     /locus_tag="VG274_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HWE07617.1"
                     /translation="MPPHRIDESVAFVPVRIAVLTVSDTRDASNDTSGDALAGLVREA
                     GHVLAARALLRDNKEQIAALLRQWVQNSEVDVVISTGGTGLTGRDVTVEAFKSVFEKE
                     IDGFSVLFHQISFETVGTSTLQSRACAGVAGGTYLFALPGSTGAVKDGWDHILKWQLD
                     VRHGPCNFVEIMPRLEEHRRKPPG"
     gene            3400..4491
                     /locus_tag="VG274_12975"
     CDS             3400..4491
                     /locus_tag="VG274_12975"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HWE07618.1"
                     /translation="MNAAAEITADSRALRGRGAQSNASGRYERETRVLLDDGWTRDDD
                     TPPSLKTEVLKDSSRTIITRNRSPDISFDRSINPYKGCEHGCVYCFARPTHAWLGLSP
                     GADFESRIFAKPNAAELLARELSAPGYVPKVIAMGTNTDPYQPIERQLRITRSILEVL
                     REFRHAVGIVTKSALILRDIDILAPMAEMGLARAALSVTTLDRKLARVMEPRAATPQR
                     RLDAIAALSRADIPVGVMFAPVIPALNDDEMETILAQASEAGAQSAGYVLLRLPLEIK
                     DLFREWLESSAPGRAKHVMSLIRQMRGGKDYDSQWHTRMKGTGPYAEMIARRFHMAVK
                     RLGLNRERTPLAIGKFRRPPRTGDQLGLF"
     gene            4701..5339
                     /locus_tag="VG274_12980"
     CDS             4701..5339
                     /locus_tag="VG274_12980"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWE07619.1"
                     /translation="MPHFIFEARVLKTMAGPVAGVDEAGRGPLAGPVVAAAVILDRKR
                     IPKGIDDSKQMQPDARAEAYGRILDVALAVGVGEASVDEIDLVNIRQATHLAMARAIR
                     ALALAPMFALVDGNDAPALPCPCDVIVDGDARSVSIAAASIVAKVTRDRMMVALHENF
                     PGYGWRTNKGYGTDEHLSALARLGPCLHHRRSFSPVHNILYGAAIEDSIVTL"
     gene            5428..>5680
                     /locus_tag="VG274_12985"
     CDS             5428..>5680
                     /locus_tag="VG274_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HWE07620.1"
                     /translation="MSKSLQLQDAIDGVLEGDCVQIMHALPEACCDLVFADPPYNLQL
                     EGELRRPDNSRVDGVDDRWDRFADFDEYDRFTRAWLAAAR"
BASE COUNT         1006 a         1863 c         1740 g         1071 t
ORIGIN      
        1 gcgtcattcg cttctctggg gtgcggcgcg catcatttcg gatcgaatgg acctgtggat
       61 gcagcgcccg ccttcccgta tcatctgcca atgccacaat ccaatccgga ttgcattctg
      121 gcgcccctgt cggccctcca atggctggtc gaggccggcg cagacgaagc cgtgggcgac
      181 gagcccgtca atcggttcga tcacgcttct gtgcgttccc ggtctgaacc tttcgaaaca
      241 accgcgtcac cgctcgcact gcggccgtcg gcggcacgtc cgagaaatgt tcctgcgaga
      301 cccgttgcgc caacctcgtc tgagggcgat cagatcggac acgcgatgga gatcgccgcg
      361 gcgtgcggca ctctcgcgga attgaaggcg gccctcgaag ccttcgaggg atgccctctg
      421 aaaaagcacg cgacccgcac cgtgtttgcc gacggcacgc cggatcgcag aatcctgttc
      481 atcggggaag cgccgggaaa ggaggaagac atcgcgggcc tgcccttcgt cgggcgtgcc
      541 ggcaagttgc tggatcgcat gcttgcctcc atcggcctcg atcggaaatc gaatgtttac
      601 ataaccaaca tcctcaattg gcggccgccc tccaatcgcg accccacgcc ggaggaagca
      661 gcgatctgtc tgcctttcct gcgcagacac atcgagctcg ttgccccccg aattatcgtg
      721 ctcctgggtg caatagcctc gcggcacgtc atgggccgga gcgaaggcat catgcgactg
      781 cgtggttcct ggctcgaata tttcgtctgc ggaagcatgg tgccggtcat gccgaccctg
      841 catccggcgt atctcttgcg gcgtccaccc gaaaagaagc tcgcatggcg cgacctccag
      901 gcgatctcgg ttaaaatcca ggccctggcc ctgcttgagc gcaacgccga ttccgggtaa
      961 gctccgcccc ttccggggaa tcgcggcaaa ggatcgggct ggtggcgcgc aatatcttcc
     1021 ttgtggcctc gtgcgtgttt tgccttgccg cgccagccgc ggtgggggcc gattcagacc
     1081 cggtttctcc tcaggagacc gcaagcaccg cgccgcacgt cctgtccctg gaagacgcgc
     1141 gccactaccg cgagattttc gccgatgaat tggacgggcg ttttgcggaa gcacgggcgc
     1201 tgatcgccga tctctccgat cgctcgctga tcggttatgt cgaggcggag cattaccttt
     1261 ccccgtacag caggcatgcg gccatcggcg atcttgccca atggcttgcg acctacgccg
     1321 agcttcccgt cgccgaacgc atccgggcgc tggccgagct tcgcaatcat catcgccgac
     1381 acaagatcct gttggccggc ttgccgggta tcccgcgccg tgccacgggc gggtatgacg
     1441 tgacggacag cctcgccaat gcgtccctcg cttcagacgc ggcgcgctcg gcgctggttc
     1501 agatcgacgc cgatgtgaag ctcgatcaac ccgctgccgc cgagatcgtc ttgcagcagc
     1561 tggcggcgtc gaatatcgca cccccctccg acatcacgag gctggcccaa cggctggcgg
     1621 catcctatct cgccgagggc caggacgacg cggcattgcg aatagcaggc agcatcgcag
     1681 gtgttgaacg cgaaagcgcc ccgttgctgg actgggatgc agggcttgcc gcgtatcgcc
     1741 tgaacaaatt cgcggatgca gcgccctatt tcgaacacct cgcgcaatcc ggagccgttc
     1801 cgggctggac gcgcagcgcc gccgctttct gggcggcgcg ttcgcatgtg gcatccggcg
     1861 atccccttcc cgttgtgtca ctcctttccg ccgccgcacg agaacagccg actttctacg
     1921 gcatgcttgc ccagcgtctg cttggccagg aaccgtcggc tgggttcagc gaccccgtgc
     1981 ttgattcggc ccgcttcgac gagatgatgc agatacccgc ggcgcatcgc gcggtggcgc
     2041 tgtggcagac cggacacacc gagggcgtcg agcaagaaat gagccgcgcc tttgccgaga
     2101 tagatctgcg cgatgccgac gcgtttgctg cgttatcgca ccgtttggat ctgccggctc
     2161 tcgagctgcg ggccagcgag gcccaggccg cgcgcggcga gctgctgtcc ggcctctttc
     2221 cggttcccgg ctacacgcca cccggagggt atcacatcga tcgctgcctt ctgctggcgt
     2281 tcgcgcgtgt cgagagccgc tttcagccac gggcggtttc catggccggc gcacggggcg
     2341 tgatgcaggt catgccgggc accgcgcagc aaatcgacgg agcgacaccg gcacggtcgc
     2401 agctcaacga tcccgcctac aatcttggat tgggcgaacg gtatctccag caattgctcg
     2461 atcggacgaa cggcaacctc tttcagctcg ctgcggccta caatgcgggc ccgggcgctc
     2521 tgatgcgctg gttggggacc agaccggaaa tcacaaacga tccgctgctt tttatcgaaa
     2581 gtctccccta cgcggaaacg cgcaactaca tcaagcatgt catgacctat tactggatgt
     2641 atgcacgccg cacgaacgag actgcgccga gtcttcagga aactgccgaa ggaaaatggc
     2701 cgctctacag gtcgaacgcc gccctgccgg tcgccgtccc tgcaccgtcc ggcacgccgc
     2761 agcctgcgac cgcggccact ctgctggtca gcgatgcctc cgcaccgcat tgacgaatcc
     2821 gttgctttcg ttcccgtccg catcgccgtg ctgaccgtct ccgatacgcg agacgcttcg
     2881 aatgatacgt cgggcgatgc actcgcgggg cttgttcggg aagccggtca cgttctcgcg
     2941 gcgcgcgcgc tgctgaggga caacaaggag caaatcgcag ctctgctccg ccagtgggtg
     3001 cagaattccg aagtcgatgt cgtgatttca accggcggca cgggcctcac cggccgcgac
     3061 gtgacggtcg aggcgttcaa gtcggtcttc gagaaggaga tcgacgggtt ctcggtgctg
     3121 tttcaccaga tcagtttcga aaccgtgggc acttcgaccc tgcaatcgcg cgcctgcgca
     3181 ggcgttgccg gaggcaccta cctgtttgcg cttccaggct cgaccggcgc ggtgaaagac
     3241 ggatgggacc acattctgaa atggcagctc gatgtgcgcc acgggccatg caatttcgtc
     3301 gagatcatgc cacggctcga agaacaccgc agaaagccgc cgggctgaaa tctggtcatt
     3361 tgttcttgct tcgttccaaa tccatgctaa gctgcgcaca tgaatgccgc ggccgaaatc
     3421 accgccgaca gccgagcact ccgcggccgg ggggcgcagt cgaatgcgtc gggacgctac
     3481 gagcgcgaga cccgcgtgct tctcgacgac ggctggaccc gcgacgacga cacccccccg
     3541 tcgctcaaga cagaagtgct caaggactcc tcgcgcacca tcatcacacg caaccgctcg
     3601 ccggacattt ccttcgaccg gtcgatcaat ccctacaagg gctgcgagca tggctgcgtc
     3661 tactgctttg cccgccccac ccatgcctgg ctgggcctgt cgcccggtgc ggatttcgaa
     3721 tcgcggatct ttgccaagcc caacgctgcc gaactcctcg cccgcgaact gtctgcaccc
     3781 ggttacgtgc cgaaggtcat tgccatggga accaacaccg atccgtacca gccgatcgaa
     3841 cgccagttgc ggatcacacg ctcgatcctg gaggtgttgc gcgaattccg gcatgccgtc
     3901 ggcatcgtga ccaaatcggc gctgatcctt cgcgacatcg atattcttgc gccgatggcg
     3961 gagatgggac ttgcgcgcgc agccctatcc gtcacgaccc tggatcgtaa gctcgcgcgc
     4021 gtcatggaac cgcgcgcggc gaccccgcag cgccggctcg acgcaatcgc cgcgttgagc
     4081 cgtgccgaca ttcccgtggg cgtgatgttt gcgcccgtaa ttcccgcgct caacgatgac
     4141 gagatggaaa cgatcctcgc gcaggcgtcg gaagcgggcg cgcaaagcgc aggatatgtc
     4201 cttttgcgtc taccgctcga aatcaaggat ctgttccgcg aatggcttga atcaagcgcg
     4261 cccggcaggg cgaagcacgt gatgtcgctg atccgccaga tgcgcggcgg caaggattac
     4321 gacagtcaat ggcacacgcg catgaagggc accgggccgt atgcggaaat gattgcgcgg
     4381 cgatttcaca tggccgtcaa acggcttgga ctgaaccggg agaggacacc actcgccatc
     4441 ggcaaattcc gccgccctcc ccgcaccggc gaccagctgg gtttgttttg attgccgagc
     4501 gcgcggaata gcgcgaatcc gaaacttact aaaacctcgt catggccggg ccgttgtccc
     4561 ggccatgacg ccgttatggt gagtcgcgga tttgcgccaa ccgcccgccg cagcggcgca
     4621 tcaacgcagg gtgtgtataa cacgcgctgt gccgcatctt gcggctttcg ggcgggcccg
     4681 cgtttttcta ctgtcggggc atgccgcatt tcattttcga agcgcgcgtc ctcaagacga
     4741 tggcaggacc ggtcgccgga gtggacgagg ccggccgcgg tccgctcgcc ggtccggtgg
     4801 tcgctgcggc ggtcattctg gaccgcaagc ggattcccaa aggcattgac gattccaagc
     4861 aaatgcagcc cgacgcacgc gcggaggcct atgggaggat tcttgatgtg gcgcttgcgg
     4921 tgggcgtggg cgaggcaagt gtcgatgaga tcgatctcgt caacatccgt caggccacgc
     4981 atctggccat ggcgcgcgcc atccgcgcgc tggcgctcgc gccaatgttc gctctggtcg
     5041 acggcaatga cgcgcccgcc ctgccatgtc cctgcgacgt gatcgtggac ggcgacgcgc
     5101 gctcggtttc cattgccgct gcctcgatcg tggccaaggt cacacgcgac cgcatgatgg
     5161 tggctttgca cgagaatttc ccgggctatg gctggcgcac gaacaagggt tacggcaccg
     5221 acgaacatct ctcggctctg gcgcggctcg gcccttgcct gcatcatcgc cgcagcttct
     5281 cccctgtgca caacatcttg tatggtgcgg cgattgaaga ctcaatcgta acactttgat
     5341 tctcaagact cttgacctgt ggactccggc gaatcactct cgccgccgca gggccaaatc
     5401 cggccttgcg caggttcggg agggggcatg tcgaaatctc tgcagctgca ggacgcaatc
     5461 gacggggtgc tggaaggcga ttgcgtacaa atcatgcacg cccttccgga ggcatgctgc
     5521 gatctggtct tcgccgaccc gccctacaat ctccaactcg aaggcgaatt gcgccggccc
     5581 gacaattcgc gcgttgacgg cgttgacgac agatgggacc ggttcgcaga ctttgacgaa
     5641 tatgaccgct ttacgcgcgc ctggctcgcg gcggcacggc
//
LOCUS       DASZMC010000638         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4660518,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000638 DASZMC010000000
VERSION     DASZMC010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4660518"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..513)
                     /gene="rsfS"
                     /locus_tag="VG274_12990"
     CDS             complement(151..513)
                     /gene="rsfS"
                     /locus_tag="VG274_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWE07621.1"
                     /translation="MRAHHSAEQPLLSRILVSLDDDKAEDVVTIDLEGRSSLADAIVI
                     ANGRSQRHVSAIAEHLARRLKEAGYGTRPVDGLASGDWVLVDAGDVIVHIFRPEVRAY
                     YDLEGMWSVEEPGRAVAN"
     gene            complement(668..1315)
                     /locus_tag="VG274_12995"
     CDS             complement(668..1315)
                     /locus_tag="VG274_12995"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWE07622.1"
                     /translation="MSAGRSAANWLKPPGPVAPGLRVGLLGGSFNPAHEGHLHVSLVA
                     TKRLGLDYVWWLVAPQNPLKPTGDMAALSERVLGAREIARGQPRICVTDIEAALGTRY
                     TVDTVSRLKRHFPQLRFVWLMGSDNLEAFHRWRNWETLARRIPIAVVMRPGTTLAPLS
                     ARAAKFFAARKVHTARQLADATPPALVILDARRNPASATEIRARGLGRRDCGVVE"
     gene            complement(1312..>1759)
                     /locus_tag="VG274_13000"
     CDS             complement(1312..>1759)
                     /locus_tag="VG274_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWE07623.1"
                     /translation="QLGMVRSDQVDFVLADLPGLIEGAHEGAGLGDRFLGHAERCAVV
                     LHVVDGTESAIVKAYRGIRRELVAYGHGLADKPEIVALNKIDAISPSLLARKKAALEK
                     AAGKDVLLMSGVSGDGVREVVRALARAVETQRTRRRTAEPARVWSP"
BASE COUNT          316 a          594 c          545 g          304 t
ORIGIN      
        1 cgcaacttcc tcgaccgata gcgccacgac gccctgctgg cggccgatcg cgcgcgcgcg
       61 tgtcagatac tcatccacca gagcgttctg cggcgtgccg cgcgccaggc ctgctgcaag
      121 gatcgcaatt cgcatatgaa actgtctggg tcagtttgcg accgcgcggc cgggctcttc
      181 gaccgaccac atgccttcga gatcgtaata ggcgcgaacc tcggggcgga agatatgaac
      241 gatgacgtcg cctgcatcga caagcaccca gtcgccggag gccagcccgt cgacgggccg
      301 cgtgccgtag ccggcctcct tgagccggcg cgccagatgt tctgcgatcg cgctgacgtg
      361 gcgctgcgat cgcccattgg cgatgacgat cgcatcggcg agcgacgaac ggccttcgag
      421 gtcgatcgtc accacgtctt ctgccttgtc gtcgtcgagg gaaaccagga ttcgggacag
      481 gagcggctgc tccgcggaat gatgtgcgcg catcggcgca gcgatcgtcg gcgcacggga
      541 cgcgccggtc gtcttacggg tcagaatgcg atcctccgtt ttcgggtcgt acgaaaaacg
      601 cgcaggccgc cgatatcttt cccggtgtca ccagccgaat gaccgcacgc ctccgtggtt
      661 gcaaatgtca ctctaccacg ccgcaatcgc gccgaccaag acctcgcgcc cggatttcgg
      721 tggcgctcgc cggattgcgc cgcgcatcga ggatgacgag agcgggcggc gtggcatccg
      781 caagctgccg cgccgtgtgg acctttctcg cggcaaaaaa ttttgcggcc ctggcgctca
      841 aaggcgccag cgtggttccc ggccgcatca cgaccgcgat gggaatgcgc ctggccagcg
      901 tctcccagtt gcgccagcga tggaacgcct cgagattgtc gctgcccatc agccagacaa
      961 atcgcagttg cgggaaatgc cgcttcaagc gcgaaaccgt atcgacggtg tagcgcgtgc
     1021 ccaacgcagc ttcgatgtcc gtcacgcaga tgcgcggctg cccgcgggcg atctcgcgtg
     1081 caccgagaac gcgttcggac aatgccgcca tatcgccggt cggcttcagg ggattttggg
     1141 gcgcaaccag ccaccacaca tagtcgaggc caagcctctt cgtcgccacg aggctgacat
     1201 ggagatggcc ttcatgcgcc gggttgaacg atccgccgag aagacccacc cgcaggcctg
     1261 gcgccacagg tccgggcggt ttcagccagt tcgcggcgga acggcccgca ctcacggcga
     1321 ccagacgcgc gcgggttctg ccgttcttcg ccgcgttctc tgcgtttcga cagcgcgcgc
     1381 caaagcgcga acgacctcgc gaacgccgtc tccgctcacg cccgacatca gcagaacgtc
     1441 cttgccggct gctttttcaa gtgccgcttt cttgcgtgcg agcaacgaag gtgaaatcgc
     1501 gtcgatcttg ttcagggcga cgatttccgg cttgtcggca agcccatgac cgtaagccac
     1561 aagttcgcgg cggattccgc gataggcctt cacgattgcg ctttccgtgc cgtcgaccac
     1621 atgcagaacg accgcgcagc gctcggcgtg accgagaaaa cggtcgccaa ggccggcgcc
     1681 ttcatgcgca ccttcgatca accccggaag atccgcgagc acgaaatcaa cctggtcgct
     1741 gcgcaccatt ccgagctgc
//
LOCUS       DASZMC010000639         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_4936466,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000639 DASZMC010000000
VERSION     DASZMC010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4936466"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1839)
                     /gene="ilvD"
                     /locus_tag="VG274_13005"
     CDS             complement(157..1839)
                     /gene="ilvD"
                     /locus_tag="VG274_13005"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWE07624.1"
                     /translation="MKLNSYTITQGRDRAPARSYLKAIGFTDEDLKKPIIGVANTWTE
                     TMNCNYKLRDLAVHVKEGIRAAGGTPMEFNTIAISDGITMGTEGMKASLVSREVIADS
                     IELVTRGHMFDGVIALVACDKTIPGAAMALLRMNIPGVVLYGGSILPGHYKGKDITVQ
                     DVFEAVGANAAGKISDFELLEIENHACPGAGACGGQFTANTMATVMELIGLSPMGTAS
                     VPQVDIRKEDVARHCGEIVMHCVRKEIKPRDICTHAAFLNAIAGVAATGGSTNAVLHL
                     LAMANEAGVNLGLEDFQTVSERTPLFVDLKPSGKYVAVDVDKAGGIPVIAQRLVEGKY
                     VDGSALTVTGHTFAEEAAKAVETPGQDVIHPLDNPIKPTGGIAILRGTLAPEGCVIKL
                     SGHKRRQHTGPARVFNREEDAMDAVTHSRIKPNDVIVIRYEGPRGGPGMREMLGVTSA
                     IQGAGLGDSVALMTDGRFSGATHGFMIAHIAPEAVAGGPIAAVEEGDTIVVDVDAGTL
                     NLEVPAEEIARRMNNWKAPAPRYTSGVFGKYIALVGSASEGAITSKQSAGAR"
     gene            1891..>2741
                     /locus_tag="VG274_13010"
     CDS             1891..>2741
                     /locus_tag="VG274_13010"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWE07625.1"
                     /translation="MRIGIDSGGTFTDFVALHDNGRLESFKLRSDPAAPARVVLEGIA
                     RIKAASADVVHGSTVATNALLERKGARTALVTTAGFEDLLAIGRQNRPELYNLTPPLP
                     SPLVPAERCFGVKERMLFDGSVLIPLEAAELTRLRAQLENAGAEAIAICLLHSYRNDE
                     HERAVLEALRGLGYVCASCEISPEFREYERASTTAINAYVGPLMDRYLGELERTSPHP
                     VAIMQSNGGLLTAAEARLQPVRTILSGPAGGIVGAVEIARLAGFDRALTFDMGGTSTD
                     VALASAE"
BASE COUNT          456 a          760 c          965 g          560 t
ORIGIN      
        1 catcggccac gttcggggtg atgccgaatc cggccgccga attctcgatg ttggcgatga
       61 cgacgtcgat agcctggact tcgcggatgt tgcgcaggtg gtcggcgacg atcatacggc
      121 cggcatggcc gaagatgtcg ccgacgaaca ggagtttcaa cgggctcctg ctgattgctt
      181 cgacgttatc gcgccttccg aagccgaacc gaccagagcg atgtatttgc cgaaaacgcc
      241 gctggtatag cgcggggcgg gcgctttcca gttgttcatg cggcgcgcga tctcttcggc
      301 cggaacttcg agattaagcg tgccggcatc gacatcgacc acgatggtat cgccttcctc
      361 gaccgcggcg atggggccgc cggcgacggc ttcgggggcg atgtgggcga tcatgaagcc
      421 gtgggtggcg ccgctgaaac ggccgtcggt catgagggcc acgctatcgc cgagacccgc
      481 gccctggata gcgctggtga cgccgagcat ttcgcgcatg ccggggccgc cgcgagggcc
      541 ttcgtagcgg atgacgatga cgtcgttcgg cttgatgcgg ctgtgggtga cggcgtccat
      601 ggcgtcttcc tcgcgattga agacgcgcgc cggaccggtg tgctggcggc gcttgtggcc
      661 ggagagcttg atgacgcagc cttcgggcgc gagtgtgccg cgaagaatgg cgatgccgcc
      721 ggtaggtttg atggggttat cgagcggatg gatgacgtcc tgaccgggag tttccaccgc
      781 cttcgccgct tcttcagcga aggtatggcc ggtgacggta agggccgagc catccacgta
      841 tttgccttcg acgaggcgct gcgcgatgac ggggatgccg cctgctttat cgacatcgac
      901 cgcgacgtat tttccgctgg gcttgagatc gacgaagagc ggggtgcgtt cggagacggt
      961 ctgaaaatct tcgagcccga ggtttacgcc cgcttcattg gccatggcga gcagatgaag
     1021 gacggcgttg gtggatccgc cggtggccgc gacgccggcg atggcgttga ggaatgcggc
     1081 gtgggtgcag atatcgcgcg gtttgatctc tttccggacg cagtgcatga cgatttcccc
     1141 gcaatgcctg gcgacgtctt ccttacgaat atccacttga ggaacagacg cggtgcccat
     1201 gggcgagagg ccgatgagct ccatcacggt ggccatggtg ttggcggtga actgcccgcc
     1261 gcaggctccg gcgccggggc aggcgtggtt ctcgatttcg agaagctcga aatcgctgat
     1321 ctttccggcc gcattggcgc ccacggcttc gaagacgtcc tgaacggtga tgtctttgcc
     1381 cttgtagtgg ccgggcagga tggagccgcc ataaaggacg acgccgggaa tattcatgcg
     1441 cagcagcgcc atggctgcgc cggggatggt tttatcgcag gccacgagcg cgatgacgcc
     1501 atcgaacatg tggccgcgcg taacgagctc gatcgaatcc gcgatgactt cgcggctgac
     1561 gagcgacgcc ttcatgcctt cggtgcccat ggtgatgccg tcgctgatgg cgatggtgtt
     1621 gaactccatg ggcgtgccgc cggcggcgcg gatgccttcc ttgacgtgaa cagcgaggtc
     1681 gcgcagtttg taattgcagt tcatcgtctc ggtccacgtg ttcgcgacgc cgatgatcgg
     1741 tttcttcaga tcttcgtcgg tgaagccgat ggctttgagg taggagcgcg cgggcgcgcg
     1801 gtcgcgtccc tgagtgatgg tgtaactgtt cagtttcatt gcggagggta aagctaatta
     1861 taaggaactg cacgttacac ttcggatttc atgcgaattg gcatcgattc gggcggcacg
     1921 tttacggatt ttgtggcgct tcatgacaat gggcgactgg aatcgttcaa gctgcggtcg
     1981 gatccggccg caccggcgcg cgtggtgctg gaaggcatcg cgcgcataaa agcggcaagc
     2041 gcggacgtgg tgcatggctc gacggttgcg accaacgcgc tgctggaacg gaaaggcgcg
     2101 cgcacggcgc tggtgacgac ggcgggtttt gaggatcttc tggcgatcgg gaggcagaac
     2161 cggccggaac tctataacct gacgcctcca ctgccttcgc cgcttgtgcc cgcggagcgg
     2221 tgtttcgggg tgaaggagcg gatgctgttc gacggctccg tgcttatccc gctggaggcc
     2281 gcggaactga cgcggctgcg cgcgcagctg gagaatgcgg gcgccgaggc gatcgctatt
     2341 tgcctgctgc attcgtatcg caatgatgag cacgagcgag cggtgctcga ggcgctgcgc
     2401 ggcctgggtt acgtatgcgc atcctgcgag atttcgcctg agtttcgcga atacgaacgc
     2461 gccagcacga cggcgattaa tgcgtatgtt ggaccgctga tggaccggta tctgggcgaa
     2521 ctcgagcgga ctagtccgca tccggtggcc atcatgcaat cgaacggagg tttgctgacg
     2581 gccgcagaag cgcgattgca gcctgtgcga accattcttt cgggcccggc gggcgggatt
     2641 gtgggcgcgg tggagattgc gcggctggcg ggtttcgatc gcgcgctcac gttcgatatg
     2701 ggcgggacct ccaccgatgt cgcgcttgcc tccgcggagc a
//
LOCUS       DASZMC010000640         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3020454,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000640 DASZMC010000000
VERSION     DASZMC010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020454"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VG274_13015"
     CDS             complement(<1..448)
                     /locus_tag="VG274_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07626.1"
                     /translation="MLFNTSRFFIFLALVLALFYALPLRARKYLLVAASYFFYASWNP
                     HFIPLLLTLTAIDFTAGIWLERSTTPSRRRAILIASLAANLGFLGFFKYYNFLASNLA
                     LALGKPTHSFFLDIVLPLGISFHTFQSISYVVDVYRGQQKAVRNPVD"
     gene            complement(460..1551)
                     /locus_tag="VG274_13020"
     CDS             complement(460..1551)
                     /locus_tag="VG274_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07627.1"
                     /translation="MPRTLRRILAAAVACLAVFALDALVFRTRYYPAYLDPNSAAGTF
                     EMILWRERQAQQKLGDNVAVTLGDSRFGIVPKLSNRLTPETGYVFRSAGVAGTDARAW
                     YYMLRDLDPQANRYRAILLGVDNYSDQDIPSEPDIDVRAIYFSIARLRVTDAFDLMNS
                     YRGYDLRLRALRGAFFKGIIYQNDFHEFLANPRKRIREVRFDRSGNEEWTYNYLGDEG
                     SLTGLTIDWDTLTAQFPASFSPYQRDTVKNFLLWKPEPQTGRLAAFRREWFGRIITRY
                     RNSRTKIIFVRLPRGPIPRPPQLTRPAGSVIREFAAQPGVLMMDEHVFEPIEDPEYFR
                     DAMHMNKAGVAIFSDLIARETGRMLGAPR"
     gene            complement(1569..>2171)
                     /locus_tag="VG274_13025"
     CDS             complement(1569..>2171)
                     /locus_tag="VG274_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWE07628.1"
                     /translation="MTMAYHAERMPAELERADYHVSSNIGRTIHAARQSAIDVRACLD
                     WLNQQGYERVGVLGTSLGSCVAFIATAHDPRVKTGIFNHVSMYFSDVVWTGLSTQHVR
                     KGFGETVTQDDLRRYWAPISPASYLDRMKNRDMRSLLIWAKHDSSFLPVYSKQVLTTF
                     REMQLPHEVFTLPCGHYTTGQFPFNLMDGLAMCRFASRWL"
BASE COUNT          429 a          504 c          857 g          381 t
ORIGIN      
        1 agtcgacggg gttgcggacg gctttctgtt ggccgcgata gacgtcgacg acataagaga
       61 tgctttggaa ggtgtggaag ctgatgccga ggggaaggac gatgtcgagg aagaaggagt
      121 gcgtgggctt gccgagcgcg agggcgaggt tggacgcgag gaaattgtag tacttgaaga
      181 agccgaggaa gccgaggttg gcggcgaggc tggcgatcag aatggcgcgg cggcgtgagg
      241 gcgtggtgga gcgctcgagc caaattccgg cggtgaaatc gatggccgtg agcgtgagca
      301 gcagtggaat gaagtgcggg ttccagctgg catagaagaa gtaactggcg gcgacgagga
      361 ggtacttgcg ggcacgcagc gggagcgcgt agaagagcgc caggacaaga gcgaggaaga
      421 tgaagaagcg cgaagtgttg aagagcatcg ccgagccgcc taacgcggcg cccccagcat
      481 gcggccggtt tcgcgcgcga tcagatcgga gaagatggca acgccggcct tgttcatgtg
      541 catggcgtcg cggaagtatt cggggtcttc gatgggctcg aagacatgtt cgtccatcat
      601 caggacgcct ggttgtgcgg cgaactctcg gatgacgctg ccggcgggcc gcgtgagctg
      661 tggcgggcgt ggaatggggc cgcggggcaa tcgaacgaag atgattttgg tgcgggagtt
      721 gcggtagcgg gtgatgatgc ggccgaacca ttcgcggcgg aaggctgcaa ggcggccggt
      781 ttggggctcg ggcttccaga gcaggaagtt cttgactgtg tcgcgctgat atgggctgaa
      841 ggacgcaggg aactgcgcgg tgagcgtgtc ccaatcgatg gttaggccgg tgaggctgcc
      901 ctcgtcgccg aggtagttgt aagtccactc ctcgttcccg gagcggtcga agcggacttc
      961 gcggatgcgc ttgcgcgggt tggcgaggaa ttcgtggaaa tcgttctggt agatgatgcc
     1021 tttgaaaaag gcgccgcgga gagcgcgcag acgcaggtcg taaccgcggt aggaattcat
     1081 caggtcgaag gcgtcggtga cgcgcaggcg ggcgatggag aagtagatgg cgcgaacgtc
     1141 gatatcgggt tcgctgggga tgtcctgatc gctgtagttg tcgacgccga ggaggatggc
     1201 gcggtagcga ttggcctgcg ggtcgagatc gcgcagcatg taataccagg cgcgcgcatc
     1261 ggtgccggcg actccggcgc tgcggaagac gtagccggtc tcgggagtga gccgattgga
     1321 gagtttgggc acgatgccga agcgggaatc gccgagagta acggcgacgt tgtcaccgag
     1381 tttctgctga gcctggcgct cacgccagag gatcatttcg aatgtgccgg cggcggagtt
     1441 gggatcgaga taagcggggt aatagcgggt gcgaaagacg agcgcgtcca gcgcgaatac
     1501 ggcgagacag gcgacggcgg ccgcgaggat gcggcggagg gtacgcggca tcggaaacta
     1561 catcgtactc atagccagcg cgaggcgaaa cggcacatgg cgaggccatc catcaggttg
     1621 aaggggaact ggccggtggt gtagtggccg cagggcaagg tgaagacctc gtgcgggagc
     1681 tgcatttcgc ggaaggttgt cagcacctgc ttggagtaga cgggcagaaa gctggaatcg
     1741 tgcttggccc agatgagcag gctgcgcatg tcgcgattct tcatgcggtc gagataactg
     1801 gcgggactga tgggcgccca gtagcggcgc agatcgtcct gcgtgacggt ttcgccgaag
     1861 cctttgcgga cgtgctgagt ggagaggccc gtccagacga cgtcggagaa gtacatagag
     1921 acgtgattga agatgccggt tttgacgcgc gggtcgtggg ctgtggcgat gaaggcgacg
     1981 caggagccga ggctggtgcc gagcacgccg acgcgttcgt agccctgttg attgagccaa
     2041 tcgaggcagg cgcggacgtc gatagcggat tggcgcgcgg cgtgaatggt gcggccgatg
     2101 ttgctagaga cgtgatagtc ggcgcgttcg agctcggcgg gcatgcgctc ggcgtgatag
     2161 gccatggtca t
//
LOCUS       DASZMC010000641         5385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3561541,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000641 DASZMC010000000
VERSION     DASZMC010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3561541"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VG274_13030"
     CDS             <1..818
                     /locus_tag="VG274_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWE07629.1"
                     /translation="QEWLPRIAEGGVVLAFAFAEPQGRYNLADLAVAAKKQGSAYVLN
                     GQKSVVIGAPFADALLVTARTAGGRREEKGVSVFLVDPRARGISTRDFPTVDGLRASE
                     ITFENVEIPAHALIGMPDDGFALLDQIVDEAIAAHCAEALGAMRVLHESTVEFSKARK
                     QFGMPIGKFQALQHRMVDMFMQVEQSASMTLMVTLKLGEGARERKKAASAAKVQIGKA
                     GRFVGQNAVQIHGGMGMTDELALGHYFKRVTMIDTLYGNVDHHLKRYASLNRV"
     gene            822..1619
                     /locus_tag="VG274_13035"
     CDS             822..1619
                     /locus_tag="VG274_13035"
                     /inference="COORDINATES: protein motif:HMM:NF006699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWE07630.1"
                     /translation="MTALLFEKSGMMVTLTINRPDSRNPLGEQGDGELFADAASRINA
                     DRGIRCVILTGAGKAFSAGGNLKAMRDKSGTFAGPGVLIRERYRSNIHRMVRALWSIE
                     APMICAVNGPAIGLGNDVTCLADLRIAADTAVFGASFLKIGLVPGDGGAWILPRIIGM
                     ARAAELFFTAETIDAATALAWGLVSKVVPAVELMTEARALADRICRQPPDVLRLTKRL
                     MREGEHSSFETLMEMSAAFQSLAHMTADHREAVEAFFEKRTSEFRGE"
     gene            complement(1771..2676)
                     /locus_tag="VG274_13040"
     CDS             complement(1771..2676)
                     /locus_tag="VG274_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWE07631.1"
                     /translation="MSIRFDGKVAIVTGAGGGLGRAHALEFARRGAKVVVNDLGGMVD
                     GTGGSSEAAQKVVAEIEAAGGKAIANGASVTDDSGVAHLVKQTLDAFGRIDVLIANAG
                     ILRDKSFAKMEIGDFSAVLDVHVMGTVKPAKAVWQLMRDQQYGRIVVTTSSTGLYGNF
                     GQANYGAAKLGLVGLMHTLKIEGAKDNIKINAICPVAATRMTENLMPPQMLEMLKPEY
                     VTPAVVYLASEDAPTGVIVTAGAGVFSAAQMIESNGVHLGPNATADDIAAAWPRISDF
                     TGARHYVTGAEQVAKIVERRQEKAG"
     gene            complement(2736..3926)
                     /locus_tag="VG274_13045"
     CDS             complement(2736..3926)
                     /locus_tag="VG274_13045"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWE07632.1"
                     /translation="MSEQARFPEIRALLGARALPPLILVLFHFCEGHGYRGAAWFDLP
                     VARGYLWVEFFFALSGFVLTYVYAQRLRQLWRPRGYLAFLQARLARLYPLHLAMLLVI
                     LFMLVTLRALAARGGYVSIYDEPWHPFCTWPTFVANLFLVQAWNLFPYLSWNGASWFV
                     SVEFLLCLLFPIYLALSRGGWRLALVLIAGGAAALALLAHPRYGLDLTFHNGIYRGMA
                     AFAMGVGFAVLHRLALGHGAASLPESVFSLAQLAILACLWYAIYHTGWSHRPSDIYTA
                     LSVIALVFVLSFDRGFLARALATRIPLRLGEWSYAIYIGQTPILQLLRHAQLHLYPAP
                     GDIVFGRPWAAWGDVWHWLEPALLVAACIVWGAILFTLIERPANTFLRSLGADRGATR
                     TASA"
     gene            4000..5262
                     /locus_tag="VG274_13050"
     CDS             4000..5262
                     /locus_tag="VG274_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HWE07633.1"
                     /translation="MPDQLIYHLQSLAGLSVILAAAWAFSENRRAFPFRTVASGLALQ
                     FALALLLLKVPAARAALLSLNVIVNALTAATRAGTSFVFGYVGGGAPPFAVTSAKGLT
                     NFAFQILPLVIVISALAALLWHWRILPFVVNTLAWILRKVMGVGGAIGLGSAATVFLG
                     MIEAPLLIRPYLAQLSRSELFMMFSVGLATVAGTVFVLYATILEPVVPGALGHILVAS
                     VLSLPGALMIGRIMIPGAADTEAQAVPSLNYRSSMDAIARGTEDGLKLWLGIVAMLLV
                     IVALVALVDIMLAPVPPIAGAPLSIERIFGWLFAPLVWLYGIPWQETATAGSLMGQKT
                     VLNEFVAYLKLAALPPGALDPRARLIMVYAMCGFANFGSVGIMIAGVSSLVPERRDEI
                     VPLAMRALLSGTMASGLTGCIIGLLPIA"
BASE COUNT          994 a         1721 c         1707 g          963 t
ORIGIN      
        1 agcaggaatg gcttcccaga atcgcggagg ggggagtggt tcttgctttc gccttcgcag
       61 aaccccaggg ccgatacaat ctcgccgatc tcgcggtcgc cgcgaagaag cagggctcgg
      121 cctacgtgct gaacggacag aaatcggtgg tgatcggtgc accctttgcc gacgcgcttc
      181 tcgtgactgc ccgcaccgcc ggtggacggc gcgaggaaaa aggcgtcagt gttttcctcg
      241 tcgacccgcg cgcgagaggc atctcgactc gcgatttccc gacagtggat gggttgaggg
      301 cgtctgaaat caccttcgaa aacgtcgaaa ttcccgcgca cgcgctcatc ggcatgcccg
      361 atgacggttt cgccctgctc gatcagatcg tggacgaagc catcgcggcc cattgcgcgg
      421 aagctctggg ggcaatgcgc gttttgcacg agtcgacggt cgaattctcc aaggcacgca
      481 agcagttcgg gatgccgatc ggcaagtttc aggctctgca gcaccgcatg gtcgacatgt
      541 tcatgcaggt ggagcaatcg gcctcgatga cgctgatggt gacgctgaag cttggcgaag
      601 gcgccagaga gcgcaagaaa gccgcgtccg cggcgaaggt ccagatcggc aaagcgggcc
      661 gtttcgtcgg ccagaacgcg gtgcagattc atggcggcat gggcatgacg gacgagctgg
      721 cgctcggcca ctacttcaag cgcgtgacga tgatcgacac gctgtacggc aacgtcgatc
      781 accatctgaa gcgctacgct tcgctcaacc gagtttgaac catgaccgcg cttctcttcg
      841 aaaaatccgg catgatggtc acgctgacga tcaatcggcc ggactcgcgc aatccgctgg
      901 gagagcaagg cgacggcgaa ctgttcgccg atgcggcctc gcgcatcaac gccgaccgcg
      961 gcatccgctg cgtcattctc accggcgcgg gcaaggcttt ttccgccggc ggcaatctca
     1021 aggcgatgcg cgacaagagc ggcaccttcg ccggccccgg cgtgctgatc cgcgagcgct
     1081 atcgcagcaa catccaccgc atggtgcgcg cgctgtggag catcgaggcg ccgatgatct
     1141 gcgccgtgaa cggcccggcg atcgggctgg gaaacgacgt gacatgtctg gccgatctgc
     1201 ggatcgccgc cgacaccgcc gtcttcggcg cgagttttct caagatcggc ctcgtacccg
     1261 gcgatggcgg cgcctggatc ctgccgcgca ttatcggcat ggcgcgcgcg gcggaattgt
     1321 tcttcaccgc cgagacgatc gatgccgcaa ccgcgctcgc atgggggctt gtttcgaagg
     1381 ttgttcctgc ggtcgaactc atgactgagg cgcgtgctct cgccgatcgc atatgccgtc
     1441 agccgcccga cgtgctgcgc ctgaccaaac gcctgatgcg ggaaggtgag cattcaagtt
     1501 tcgaaacgct gatggaaatg tccgccgcct tccagtccct cgcccacatg accgccgacc
     1561 atcgtgaagc ggtggaagcc ttcttcgaaa agcgaacatc ggagttcagg ggggagtaga
     1621 attgccccct ccggcgcttc gcgccacctc ccccacgcaa gcgcggggga ggaaaacgcg
     1681 atcgcgcacg cccaatactt tcctcccccg cgcgaagcgt gggggaggtg cccgcgaagc
     1741 gggcggaggg ggcaagcggc cccgcgcgat tcaacccgcc ttctcctgcc gtcgttcaac
     1801 gatcttcgcg acctgctcgg cgccggtgac gtagtgtctg gcgcccgtaa agtcggagat
     1861 gcgcggccag gcggctgcga tgtcgtccgc tgttgcgttg gggccaagat ggactccgtt
     1921 cgactcgatc atctgcgccg cggagaacac gccggcgccg gcagtgacaa tcacgccggt
     1981 gggcgcatcc tcgcttgcga gatagacgac ggccggggtc acatattcgg gcttcagcat
     2041 ttccagcatc tgcggcggca tcaggttttc ggtcatgcgg gtggcggcca ccggacagat
     2101 cgcattgatc ttgatgttgt cctttgcacc ttcgatcttg agcgtgtgca tcagaccgac
     2161 gaggcccagc ttggcggctc cgtaatttgc ctggccgaaa tttccgtata gcccggtgga
     2221 tgaagtggtg accacgatgc ggccatattg ctggtcgcgc atgagctgcc acacggcttt
     2281 cgcgggcttc acggtgccca tcacgtgcac gtccagtacc gccgaaaaat ccccgatttc
     2341 catcttggcg aagctcttgt cgcgcagaat gcctgcattg gcgatgagca cgtcgatccg
     2401 tccgaacgcg tcgagcgtct gtttgacgag atgtgcgacg cccgaatcgt cggtgaccga
     2461 tgcgccgttc gcaatcgctt tgccgccggc cgcttcgatc tccgcgacga ccttctgcgc
     2521 tgcttccgac gagccgccgg tgccatccac catgcccccc agatcgttga cgacgacctt
     2581 cgcgccgcgg cgtgcgaatt cgagcgcatg cgcgcgcccc agcccgccgc ccgcgccggt
     2641 gacgatcgcc accttgccgt cgaaccgaat gctcatgtga aatctccgcc ccggaattgc
     2701 gttcgggcga gtgatggccg aggcgcgtca ggcggtcaag ccgaggctgt ccgcgtagcg
     2761 ccacggtccg cgcccagact gcggagaaag gtgttggccg gccgctcgat gagagtgaaa
     2821 aggatcgctc cccacacaat gcacgccgcg accagcaacg ccggttcgag ccaatgccat
     2881 acgtcccccc acgctgccca cggcctgccg aacacgatat cgccgggtgc aggatagaga
     2941 tgcagctggg cgtggcgcag caactgcaga atgggagtct gtccgatata gattgcgtag
     3001 gaccactcgc cgagcctgag cggaatccgc gtcgccaacg ccctggcgag aaagccgcgg
     3061 tcgaacgaca gaacgaaaac gagcgcgatg acggaaagag cggtgtagat gtccgaaggc
     3121 cgatgcgacc agcccgtatg gtagatcgcg taccagagac aggcgaggat cgcgagctgc
     3181 gcgagcgaga aaaccgattc cggcaatgag gccgcgccgt gcccaagcgc gaggcggtgg
     3241 agcacggcga atccgacgcc catcgcgaac gccgccatgc cgcgatagat gccgttgtga
     3301 aaggtgaggt ccagcccgta gcggggatgg gccaacagcg cgagcgctgc tgctccgcca
     3361 gcgatcaaca ccaatgcaag ccgccagccg ccgcgcgaca gggcgagata gatcgggaac
     3421 agcagacaca gcaggaactc gacgctcacg aaccacgagg caccgttcca cgacagataa
     3481 ggaaacagat tccacgcctg aacgaggaag agattggcga cgaaagtcgg ccaggtacaa
     3541 aacggatgcc agggctcgtc atagatcgac acgtagccgc cgcgcgcggc cagtgcgcgc
     3601 aatgtaacga gcataaaaag aatcacgagc agcatcgcga gatgcagcgg gtagaggcgc
     3661 gccagccgcg cctggagaaa cgccaggtag ccgcgcgggc gccagagctg ccgcagccgt
     3721 tgcgcataga cataggtcag cacaaagcct gacagggcga agaagaactc gacccagaga
     3781 tagccgcgcg ccacgggcag gtcgaaccag gctgcgccgc gatagccgtg accttcgcaa
     3841 aagtggaaca gcaccaggat cagtggcggc aatgcacgcg cgcccagcag cgcgcgaatt
     3901 tcggggaatc tggcttgctc gctcatgcgc tcgcgcgaat cattgtactg attgtggcac
     3961 cccggatcgc cgctctccac ggcgccggca ggggcagctg tgccggacca gttgatctat
     4021 catctccagt cgcttgccgg gctgtcggtc attctggcgg ccgcgtgggc attctccgag
     4081 aatcggcgtg cgtttccatt ccggaccgtc gcatcgggtc ttgcccttca gtttgcgctg
     4141 gcgcttcttt tgctgaaagt ccctgccgcg cgcgccgcgc tcctgtcgct caacgtcatc
     4201 gtcaacgcgt tgactgcggc aaccagggcc ggcacatcct tcgtgttcgg ctatgtgggc
     4261 ggtggcgctc cgcccttcgc agttaccagc gcgaaaggcc tgacgaattt cgcctttcag
     4321 atcctgccgc ttgtcatcgt catttcggcg ctcgccgcct tgttgtggca ctggcggatc
     4381 ctgccgttcg tggtcaacac cttggcctgg atcctccgca aggtgatggg cgtgggcggc
     4441 gcgatcgggc tcggctcggc agccacggtg tttctcggca tgatcgaggc gccgcttctc
     4501 atccgaccct atctcgccca gctgtcgcgc tcggaactgt tcatgatgtt ctcggtcggg
     4561 cttgccacgg tcgcaggcac agtgttcgtg ctctacgcca cgatcctcga acccgtggtg
     4621 ccgggcgccc tcggccatat tctggtggct tccgtcctgt ccctgccggg cgccctcatg
     4681 attggccgca tcatgatccc cggcgcagcg gacaccgaag cccaggctgt gccgagcctc
     4741 aattaccgct ccagcatgga cgcgatcgcg cgcggcacgg aagacgggct caagctctgg
     4801 ctcgggatcg ttgcgatgct gctggtgatc gtggctctgg tggcgctcgt ggacatcatg
     4861 ctcgcgccag ttccgccaat tgccggcgct ccgctcagca tcgagcgcat cttcgggtgg
     4921 ctcttcgcgc cactcgtctg gctttacggg attccatggc aggaaaccgc cacggcgggc
     4981 tcgctcatgg gccagaaaac ggtgctgaac gaattcgtcg cctatttgaa actcgccgcg
     5041 ctgccacccg gcgcgctcga cccccgcgcg cgcctcatca tggtctatgc catgtgcggc
     5101 ttcgccaatt tcggttccgt cggaatcatg atcgcgggcg tctcgagcct cgttcccgaa
     5161 cgccgcgacg agatcgtacc tctcgccatg cgcgcactcc tctccggcac catggccagc
     5221 gggctgacgg gctgcattat tggactcttg ccgattgcat agatgcggaa ctgaacccgg
     5281 ggggttggtc cataagatta gctcgctaaa agattttaac gcagaggacg cggaggtagc
     5341 gcaggggacg cagcggaaaa aatggcgcgc gaacgttttc tcagc
//
LOCUS       DASZMC010000642         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3561583,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000642 DASZMC010000000
VERSION     DASZMC010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3561583"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VG274_13055"
     CDS             complement(<1..288)
                     /locus_tag="VG274_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07634.1"
                     /translation="MKDRMFRRIFLPLGLAVGGLVLAALVGAVVYVYSTTRDLPSVEA
                     LKNSQPPVTTRVYAGNGTLLGEYARERRICVPITFVPKLVVNAFTSAEDKNF"
     gene            complement(335..1678)
                     /locus_tag="VG274_13060"
     CDS             complement(335..1678)
                     /locus_tag="VG274_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWE07635.1"
                     /translation="MGDVLGQDSAMMQRFGLGVGLLVLGTVLGAFAGSGAFARPLRAE
                     IPLAKPAPMGAAETDAEPVVMSAHIDERADRTRFIVELSDPVKIRVFTLANPNRVVID
                     MPEMLWRLTSEDRPSGRGAVGSYRYGLFRPGNSRFVIDLRRPAKISEPLILPPQAGNG
                     FRIILDLQTTTQAKFASNAGWPADLRAREATAERIATTTPTASMTAADADLEKRVVVL
                     DAGHGGIDTGASGVDGVIEKDLVLDEVLRLRKKLLRRGYSVHLTRDSDLYIPLLERVN
                     VARALHADLFVSLHADSNSDPSVYGASVYTLSEAGSDREAAALARKENLSDVIAGVDL
                     SGQNSPVASILIGLAQRDTMNRSVRFAQSAVAQLAHATDILPRDPHRSAGFVVLKAPD
                     VPAVLIELGYLSNPRDCAQMATDSWRDRVALALANAVDRQLRAAAPVTETAQETE"
     gene            2105..>3217
                     /locus_tag="VG274_13065"
     CDS             2105..>3217
                     /locus_tag="VG274_13065"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HWE07636.1"
                     /translation="MPKRMIIDASHREETRVVVLDGQKVEEFDFEAAAKRPLKGNIYL
                     AKVTRVEPSLQAAFVEYGGNRQGFLAFAEIHPDYYQIPIADRMALLAEQEAEARRRAD
                     DDIESVEITSIPAQESAAEAAASVQDVSQMEAFPNEERPDADSSIHALNEQNPDEHVY
                     QQEPTLISESDLEEGAPGTDGAQTTPNAAGGHDEDEVDAAAPAQSKLQTARPAQRWTR
                     KRHYKIQEVIKRRQVILVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTPRGGGISRK
                     ITSATDRTRLKSAAQSLELPEGMGLIIRTAGENRTKAEIKRDYEYLLRMWDTIRELTL
                     KSNAPACVYEEGDLIKRAIRDLYNKDVTEIVVEGDEG"
BASE COUNT          688 a          992 c          983 g          554 t
ORIGIN      
        1 gaagttcttg tcctcggccg acgtgaaggc gttcacgacc agcttcggca cgaaggtaat
       61 cggcacgcag atgcgtcgtt cgcgcgcata ttcgccgagc agcgttccgt tgcccgcgta
      121 gacgcgcgtg gttaccggcg gctgagaatt ctttagggcc tcgacactcg gcagatcccg
      181 tgtcgtggaa tagacataca cgacagcgcc caccagcgcg gcaagcacaa gtccgccaac
      241 cgcgagtccc agtgggagaa agatgcgacg gaacattcta tccttcacgc ccagccttga
      301 tgcagcgcga ttatggcctt ggcaaggcgt tccgtcactc ggtctcctgc gcggtctcgg
      361 tcaccggagc agccgcccgc aattgcctgt ctaccgcgtt ggcgagggcc aaggcgaccc
      421 tgtcacgcca gctatccgtc gccatctgcg cgcaatctct tgggttggaa aggtatccca
      481 gttcgatgag cactgccgga acgtcgggag cttttagcac gacaaagccc gccgagcgat
      541 gcgggtcacg cggcaaaatg tccgtcgcgt gcgccaactg ggcgaccgcg gattgggcga
      601 agcggacgga cctgttcatc gtgtcccgct gggcaagacc gatcaagatt gatgcgacag
      661 ggctgttttg accggagaga tcgacgcctg caataacatc cgacaggttc tctttccgcg
      721 cgagggccgc ggcctcccgg tccgacccag cttccgacag ggtgtagacc gacgcgccgt
      781 agactgacgg atcggaattg gagtccgcgt gcagtgaaac gaaaagatcc gcgtgcaatg
      841 cacgcgccac attcacccgt tcgagaagcg ggatgtagag atcgctgtcc cgtgtaaggt
      901 ggacggaata gccgcgccgc aagagctttt tccgcagccg gagcacctcg tcgagaacga
      961 gatccttttc gatgacgcca tccacgcccg atgcgccggt atcgataccg ccatggccgg
     1021 catccagcac gaccacgcgc ttttccaaat cggcgtcagc ggcggtcatc gaagcagtgg
     1081 gagttgtggt tgcaatccgc tccgccgtcg cctcccgggc gcgcagatcg gcaggccatc
     1141 ctgcgttcga cgcgaatttc gcctgcgtgg tcgtctgaag gtcgaggatg atgcgaaagc
     1201 cattgcccgc ttgcggcgga agaatcaaag gctccgaaat tttggcgggc cggcgcagat
     1261 cgatcacgaa gcgggaattt ccagggcgga aaagaccgta gcgatagctg ccgaccgcgc
     1321 ccctgccgct cggacgatct tcgcttgtca gccgccagag catttctggc atgtcgatga
     1381 cgacgcgatt cggattggcc agcgtgaaaa cgcgaatctt gacgggatcg gaaagctcga
     1441 cgatgaagcg ggttcgatcg gcgcgttcat cgatatgggc gctcatgacc accggctccg
     1501 catcggtttc cgccgcgccc atgggggcag gcttggccaa cggaatctcc gcgcgaagcg
     1561 gccttgcaaa cgctccgctc cccgcgaatg ccccaaggac ggtccccagg acgagcagtc
     1621 ccacaccaag cccgaatcgc tgcatcatcg ccgaatcttg ccccaatacg tcgcccattc
     1681 gcacatggtc tgtgcctgcg ccatcgccag cttcaaaaaa tatggtgaat tctccattaa
     1741 caattccggg ctgacctctt gccgcggcgg gggccgccac ctaaaagaga ttcgtcctgt
     1801 ggcgatgacc gcgccggaca ccctcttcgg gtcccggaat ggccgggacc ggaagactac
     1861 cgttccttga acggcgaggg atccaggcaa caggcggttt cacaggcctc gtgcacgcgc
     1921 acgaccgcga agtgaattgc gatgacctac acaacccagc cgagatcgtt tcgtccagcg
     1981 gcccttctgc cggcgcaagg tcttgcgggc caacattcaa cgcgaccggt gtccccattg
     2041 agctgcggca ctgcccgaaa ggcgtgctcc cgcttgcgtg cgacgccgtc gcccggagat
     2101 tcgaatgcca aagcgtatga ttatcgatgc cagccaccgg gaagaaaccc gggtcgttgt
     2161 tctcgacgga caaaaagtcg aagagtttga tttcgaggcc gcagcaaaac ggcctctcaa
     2221 gggaaacatc tacctcgcca aggtaacgcg ggtcgagccg tcgctgcagg ccgcgtttgt
     2281 cgaatatggc ggcaatcggc agggatttct ggcttttgca gaaatccatc cggattacta
     2341 tcaaatcccg attgccgatc gcatggcgct gctcgcggaa caggaagccg aagcgaggcg
     2401 tcgcgccgac gatgacattg aatccgtcga gatcaccagc atccctgcac aggagtcggc
     2461 agccgaggcg gccgcctcgg tgcaggatgt atcgcaaatg gaggcatttc caaacgaaga
     2521 acggcccgac gcggattcct cgattcatgc cctcaacgag caaaacccgg atgagcacgt
     2581 ctatcagcaa gagccgacgc tgatttccga gtctgatctg gaggaggggg cgccggggac
     2641 cgacggggcg cagacaacgc cgaatgcggc cggcggacat gatgaagatg aagtcgatgc
     2701 cgcggcgccg gcgcaaagca aattgcagac agcgcggccc gctcaacgct ggacccgcaa
     2761 gcggcactac aagatccagg aagtcatcaa acgccgccag gtgatcctgg tacaggtggt
     2821 caaggaggag cgcggcaaca agggcgctgc cctcacaacc tatctttcgc tcgccggccg
     2881 atactgcgtt ctcatgccaa atacgccgcg gggcggcggc atatcgcgca aaatcacgag
     2941 cgcgacggat cgcacccgtc tgaaatcggc cgctcaatcg ctcgaactgc cggaaggaat
     3001 gggtctgatc atacgcaccg cgggcgagaa ccgcaccaaa gcggagatca agcgggacta
     3061 cgaatatctc ctgcgcatgt gggacacgat ccgggagctg acgctgaaat cgaatgcgcc
     3121 tgcctgcgtg tacgaggaag gcgatctgat caagcgcgcg atccgcgatc tctacaacaa
     3181 ggacgtcacg gagatcgtcg tcgaaggcga tgagggc
//
LOCUS       DASZMC010000643         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3021937,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000643 DASZMC010000000
VERSION     DASZMC010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3021937"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            586..1623
                     /locus_tag="VG274_13070"
     CDS             586..1623
                     /locus_tag="VG274_13070"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HWE07637.1"
                     /translation="MNANSRRLTGPAVAVFGAAGHTGRFVVAELQRRGIVPIAVARDA
                     AALAANFPGNEILRRPASIDNAESLDRALDGARTIINCAGPFLDTADAVAAAALRAGI
                     HYLDVTAEQPSARATFDNYDSATRDAGIVFVPAMGFYGGFADLLVTAALGEWDNAETI
                     EIMIGLDSWHPTRGTRITGERNTARRMVVTGGQLAPLPLPAAERHWEFGDPLGSQTMV
                     EVPLSEIILIARHVKTVELHTYLNTVALDDIRDPATPAPEPVDASGRSPQRFVVDAVV
                     KRAGERRRAVARGRDIYAFSAPLVCEVAERLLKGQFSNAGAHAPGAILDARDVLEALV
                     PDHLTFEIRVS"
     gene            complement(1748..2032)
                     /locus_tag="VG274_13075"
     CDS             complement(1748..2032)
                     /locus_tag="VG274_13075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWE07638.1"
                     /translation="MGEPARRSELWLRPALTWIALCVLLAATCTLAYVPMGSGNLPVS
                     LGIAAIKAALVGVVFMRLFEPNPINRLAAAVGPIWVFVMFLLLGADYFTR"
     gene            complement(2025..2660)
                     /locus_tag="VG274_13080"
     CDS             complement(2025..2660)
                     /locus_tag="VG274_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWE07639.1"
                     /translation="MSDAFLRPPFANLTRQHQAVSFGMWIFLVSEILLFSGLFAGYAT
                     YRGLYPQGFLAAGRETSIIYGTANTAILMTSSFTIAVAGRAARADFGRMSRRLLIATF
                     LLGLLFLVLKGFEYREDIEKHLVPGSHFALPQIGAQLFFSFYWIMTGVHALHVSGGLA
                     AVLRLIVASRRDPRWLSGSASEDATALYWHLVDVIWIVLYPLLYLAGRAHG"
     gene            complement(2657..>3043)
                     /locus_tag="VG274_13085"
     CDS             complement(2657..>3043)
                     /locus_tag="VG274_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWE07640.1"
                     /translation="LYPELWARIAAVLMFVGFNLTFFPQFVMGYLGMPRRYHVYAPEF
                     QVYHVLSTAGAAVLGVAYFMPLFYLVHSLRAGARAGANPWNAKGLEWQAPSPPPRENF
                     LAEPDVGQPYDYHPEEGPDALGNEAA"
BASE COUNT          621 a          950 c          947 g          525 t
ORIGIN      
        1 ccgcgcaatc catcgcccgc ttcgcgccgg attggccgat aagaatcctg tcgagagatg
       61 cgccatcgac ggcgcaggaa tttgtggaat tgtggggtgc gatccgtcgg ggcaagaacg
      121 cgtagcggtc atccgcacgt atctgacgga gctttcgcgc ccctgccgtt ttgaacctgt
      181 gactttccgg cgtcggccgc tatctattcc aggacacttc aaaagggtcg ctgggccgcc
      241 ctttatacga aatttttttg acggtatttt taacggtatg gctcgatttt atccaattaa
      301 atcaatggct gcgaatccta gtcccccagc caatcgctgc cgaaggcggc gattgtcctc
      361 cgcgaaagcg aaggacccag tttaggaatc cgggcatagc acaacagagc ggtcgcgcgg
      421 atattaattg gtggacgcga gcaacatttc ccgtatctgg gctttggaca tacgctccag
      481 tcgcatcgtt cctgtcggca ccaaccagga tcggcgactg tatcgacatc gcccgggtcc
      541 atcgacgaga acaagcagtt ctgcaacgaa tgggagaggt tgataatgaa cgcaaacagc
      601 aggcgattga ccggacccgc cgtggcggtc ttcggcgccg ccgggcacac cggccgcttc
      661 gtcgttgcgg aattgcagcg ccgcggaatc gtgccgattg ccgtggcccg cgacgccgcc
      721 gcactcgccg cgaattttcc cgggaacgaa atccttcgcc gcccggcgtc catcgataat
      781 gcggagtcgc tcgaccgggc gcttgacgga gcccggacga tcatcaattg cgcaggtccg
      841 tttctcgaca cggccgatgc cgtcgccgcg gccgccttgc gcgccggcat ccattatctc
      901 gacgtcaccg ccgagcagcc gagtgcacgg gcgacgttcg acaattacga tagcgccacg
      961 cgcgatgcgg gcatcgtttt cgtcccggcg atggggttct acggtggctt cgccgacctg
     1021 ctcgtcacgg ccgcgctggg cgaatgggac aatgccgaaa cgatcgagat catgatcggt
     1081 ctcgacagct ggcatccgac acgcggcacg cgcattacgg gggagaggaa taccgcgcgg
     1141 cgcatggtgg tcaccggggg acaattggcg cccctgccat tgcctgccgc cgagaggcat
     1201 tgggagttcg gcgacccgct cggcagtcag accatggtcg aagtgccgct ttccgagatc
     1261 attctgatcg cccggcacgt gaagactgtc gaactccata cctatctgaa caccgtcgcg
     1321 ctcgacgaca tccgcgatcc ggccactccg gcgccggaac cggtggatgc gagcgggcgg
     1381 tcgccgcagc gcttcgtggt cgatgcagtc gtaaagcgtg ccggagagcg ccgtcgtgcc
     1441 gtcgcgcgcg ggcgcgatat ctacgctttt tcagcgccgc tcgtctgcga ggtggccgag
     1501 cgcctgctca agggccagtt cagcaatgcc ggcgcacacg cgccaggcgc gatcctcgat
     1561 gccagggacg tgctcgaagc cctcgtgccc gatcatctca cattcgagat aagagtgtcg
     1621 taatcaatca cggcgcttgc agcgcaacga gatcgcatga gtcaatacgc gaaagtctat
     1681 cgcaggtcct tttgggcgat gtctggctcc gccttggcct gcattcagca gctttggaaa
     1741 cataagacta tctcgtaaag tagtccgctc ccaacaacag aaacatcacg aatacccaaa
     1801 tcggaccgac ggcggcggcc agccggttga tcgggttggg ctcgaacagc cgcatgaaga
     1861 ccacgccgac aagcgccgcc ttgattgccg caatgccgag gctgacgggc agattgccac
     1921 tccccatcgg cacataggcg agcgtgcagg tggctgcgag caggacgcag agcgcaatcc
     1981 aggtcagcgc gggcctcagc cacaattcgc tccgccgcgc gggttcaccc atgggcgcgc
     2041 cccgccagat agaggagcgg ataaagaacg atccagatca cgtccaccag gtgccagtac
     2101 aatgccgtgg cgtcttcgga cgcgctcccc gaaagccagc gcggatcgcg acggcttgcc
     2161 acgatgagcc gcaagacggc ggcgagcccg ccgctcacat gcagcgcgtg gactccggtc
     2221 atgatccagt agaacgaaaa gaacagctgc gcgccgattt gcggcagcgc gaagtgcgag
     2281 ccgggcacca ggtgcttctc gatatcctcg cgatactcga atcccttgag caccagaaac
     2341 agaagcccaa gaagaaaggt tgcgatcagc aggcgccggg acatccggcc gaagtcggcg
     2401 cgcgcggcgc ggcccgccac cgcgatcgtg aaactgctcg tcatcaggat cgcagtattg
     2461 gcggtcccgt agataatgct ggtttcacgg cccgcagcga gaaagccctg cgggtaaagt
     2521 ccgcgatagg tggcgtatcc tgcgaacagc cccgaaaaga gaagaatttc gctgacgaga
     2581 aatatccaca tcccgaacga gacggcctgg tgctggcgcg tcagattcgc gaagggcggc
     2641 cgcagaaagg cgtcgctcat gccgcctcgt tgccgagcgc gtcaggccct tcctccggat
     2701 gatagtcgta gggctggccg acgtccggct cggcgagaaa attctcgcgg ggcggcggcg
     2761 acggcgcctg ccattcaagg ccctttgcgt tccatggatt ggcgccggcc ctggcgccgg
     2821 cgcgcagcga atgaacgaga tagaacagcg gcatgaaata ggccacgccg agcaccgcgg
     2881 cgcccgctgt cgagagcacg tggtacacct gaaattccgg ggcgtagacg tgatagcgcc
     2941 gcggcatgcc gagatatccc atcacgaact gcgggaagaa ggtcagattg aacccgacga
     3001 acatcagcac ggctgcaatg cgcgcccaca gttccgggta cag
//
LOCUS       DASZMC010000644         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5213464,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000644 DASZMC010000000
VERSION     DASZMC010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5213464"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            817..2169
                     /locus_tag="VG274_13090"
     CDS             817..2169
                     /locus_tag="VG274_13090"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWE07641.1"
                     /translation="MPPESPLLTDLYQLNMIQAYLDHGDTQTAVFEFFVRTLPRRRGF
                     LLAAGLDQALDYLENLRFSADDIAWLKSTGRFKPNMLDYLAAFRFTGNVHAVPEGTAF
                     FASEPILRITASLPEAQFVETRLINILHFQCLVAAKAARCILAAPGKLLVDFGFRRAH
                     GAEAGLMAARASYLAGFAGTATVLAGERFGIPLYGTMAHSFIEAFDDETAAFEAFARS
                     RPDNLTLLLDTYDTEAAARKVVTLAPRLKAAGIAIRSVRLDSGDFAALARSARQILDQ
                     GGLTAVAIFASGGLDEDSLAALVRQGAPIDGFGVGTSLTTSADVPNVDCVYKLQEYAG
                     LPRRKKSEHKATWPGRKQVWRRYGSDGRMAGDILGLEGHDKSGEPLLALVMKDGRRLR
                     PPEPLADIKLRTKRELDRLPEPLRRLDLDATYPIEVAPELRDLAAEVDRRLQHNGAQP
                     "
     gene            2169..2813
                     /gene="pncA"
                     /locus_tag="VG274_13095"
     CDS             2169..2813
                     /gene="pncA"
                     /locus_tag="VG274_13095"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HWE07642.1"
                     /translation="MAAKTRAKFGDDGVLIVVDVQNDFCRGGALAVPRGDEIIPIINS
                     LAARFRNVVLTQDWHPRGHSSFASSHPGKKPFETITAYYGPQVLWPDHCVQNTRGAAF
                     HKALDIPHTALIVRKGMHSAIDSYSTFYENDHKTPTGLVGYLRERGLSRLFLAGLAFD
                     FCVRYSAEDARHQGFDAFVVEDACRNIDLDGSLAATHTSLAAIGVPCINTREFS"
     gene            2954..3166
                     /locus_tag="VG274_13100"
     CDS             2954..3166
                     /locus_tag="VG274_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07643.1"
                     /translation="MRHATAAPASRLPSIMMPCPVCAGRMAYHGCRAVAPDVEDTVYA
                     CKSCGAEVIRTAMRHSGGADKREAAA"
     gene            complement(3163..>3684)
                     /locus_tag="VG274_13105"
     CDS             complement(3163..>3684)
                     /locus_tag="VG274_13105"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07644.1"
                     /translation="LFPMVEQVRRAVGWVYRNAEKFGGDRNRLHLVSHSSGSHLSGCT
                     VTHDWAKDGLPLDILKGATLSSGMYDLKPVRLSKRSKYVKFTDEMEHELSAMRHLDRL
                     HTPLILSYGTYETPEFQRQTRDFATAVKAAGKPVELLVGEGFNHFEMLETLANPFGLL
                     GRAVLGQMRLPLV"
BASE COUNT          677 a         1199 c         1132 g          676 t
ORIGIN      
        1 cgcccgcgaa tcatgatttt tgactcgcac gcttcccaag gggttctcat cagcaacaac
       61 atcacgacga aaagtagcgt tgaatcaaac agaagcgcgc gccgaatgat tcggcttgac
      121 tttgcccgcg cggcgactaa aagcgccggc ctctcgctcc tcgtgcaagg acgtcagacg
      181 gtgactccgg ctggcgggag cgggatgcgg tgcccgcgcg caaagggttc gtccccattg
      241 cactcgggcg gtatcggaca tcatgtccgc cggcactatg accggcgtgc gaggtgctcg
      301 ctgtaaccgg ggttggcgcc ggcgggagac ccccgccatc gaggcgccag ctccgcgaaa
      361 cctggtccga cgccgcacct tgggatgcgt gagtcccaag ggcagaaccg ccgtggaacg
      421 ccgacaggcg agcgcgccct gcagggtgcg cgctgcgcct tacggtgcag caggtagacc
      481 cgcgtctgtc ggcgttctgc ttccttttct ttccgtccgt tcgttcgtgc gccgcaggcg
      541 accttgagcc acttggtctc tccacggccg aattgctctg acgaaggctg aagtgcggcg
      601 tcgtttttgg aaatggcttt tttgcgcatg atccgatccg aaaaccggaa accacttttc
      661 gggatcatgc gcctcatagc tcggacgcgc aaaaaccgcg ccgcgagaac gggagcgctt
      721 gtctttcatt cgccccatcg gggggagtgc gggtgcgcca taggcgccgt actctggtaa
      781 tctccccagc cgctgtcagc accccgaagc gatccgatgc ctccagaaag tccgctgctc
      841 accgacctct accagctcaa catgatccag gcctatctgg atcatggcga cacccagacc
      901 gcggtgttcg agtttttcgt gcgtacgctg ccgcggcgtc ggggatttct gctcgccgcc
      961 ggcctcgacc aggcgctcga ttatctggag aacttgcgct tctccgcgga tgacatcgca
     1021 tggctcaaga gcaccggtcg tttcaagccc aatatgctcg attatctcgc ggcattccgt
     1081 ttcaccggca acgtccacgc cgtgccggaa ggcaccgcat tcttcgccag cgaaccgatc
     1141 ctgcgtatca ctgcatcgct gccggaagcg caattcgtcg aaacccggct gattaacatc
     1201 ctgcatttcc agtgcctggt cgccgcgaag gccgcgcgct gcatcctggc cgcgcccgga
     1261 aagctcctgg tcgatttcgg cttccgccgc gcgcacggcg ccgaggccgg attgatggcg
     1321 gcccgggcga gctatcttgc gggcttcgcc ggcaccgcga cggtgctcgc cggcgagcgc
     1381 ttcggcatcc cgctctacgg caccatggcg cactcgttca tcgaagcctt cgacgacgag
     1441 accgcggcgt tcgaggcgtt tgcgcggtcg cggcccgaca atctcacatt gttgctcgat
     1501 acctacgata ccgaagccgc agcgcggaaa gtcgtgacgc tggcaccgcg actgaaggcc
     1561 gccgggatcg cgatccgcag cgtgcgactc gacagcggcg atttcgctgc gctggcacga
     1621 agcgcgcgac aaatccttga tcaaggcggc ctcaccgcgg tagcgatctt tgccagcggc
     1681 gggctcgatg aggattcgct cgccgccctc gtgcgccaag gtgcgccgat cgatggtttc
     1741 ggcgtcggca cgagcctgac cacgtccgcc gacgtgccca acgtcgactg cgtttacaag
     1801 ctgcaggaat atgccggcct gccgcgccga aagaagtcag agcacaaagc cacctggccc
     1861 ggacgcaagc aggtatggcg ccgctacggc tccgacggtc gcatggcggg cgacattctg
     1921 gggctcgaag ggcacgacaa gagcggcgaa ccgctcctcg cgttggtgat gaaggacggc
     1981 cggcgccttc gtccaccgga gccgctcgcc gatatcaaac tgcgcaccaa gcgcgagctc
     2041 gaccgtctgc ccgaaccctt gcggagacta gatctcgacg cgacataccc catcgaggtc
     2101 gctcccgaac tgcgagacct tgccgcggag gtcgaccgcc gtctgcaaca caacggggcg
     2161 cagccctgat ggcagccaag acgcgggcaa aattcggcga cgacggcgtt ctcatcgtcg
     2221 tcgacgtgca gaacgatttc tgtcgcggcg gcgcgctggc ggtgccgcgc ggcgacgaga
     2281 tcatcccgat catcaacagt ctcgcggcac gcttccgcaa cgtggtgctg acgcaggatt
     2341 ggcatccgcg cggacattcc tcgttcgcgt cgtcacatcc gggcaagaag ccgttcgaga
     2401 cgatcaccgc gtattacggg ccgcaagtgc tgtggccgga tcattgcgtg cagaacacgc
     2461 gcggcgcggc ttttcacaag gcgctcgaca ttccgcatac ggcgctgatc gtccgcaagg
     2521 gcatgcacag cgccatcgat tcctattcga cgttctatga gaacgatcac aagacaccga
     2581 ccgggcttgt cggctaccta cgcgaacgcg gcctgtcgcg actgttcctc gccgggctgg
     2641 cgttcgattt ttgcgtccgc tattcggccg aggacgcccg ccaccagggc ttcgatgcgt
     2701 tcgtggtgga ggacgcctgc cgcaacatcg acctcgacgg ctcgctagcg gcaacgcaca
     2761 caagtctcgc cgcgatcggc gtgccttgca ttaacacacg agaattctct taatttttca
     2821 ttggcttacg gagaaaacaa aggtccaagg gtaatgcggc gatgcggcac gaactgtgtg
     2881 caatgcacaa attaccttga ctcaccgtgg caacaggggg gaaagtcaca aaagcgtcaa
     2941 tcggggttca ctcatgcgcc atgctacggc cgctcctgcg tcgagactgc cgtcgatcat
     3001 gatgccctgc cccgtttgtg ccggacggat ggcctatcac ggctgtcgcg cggtcgcgcc
     3061 cgatgtcgaa gacaccgtct atgcgtgcaa gagctgtggc gccgaagtga tccgcaccgc
     3121 gatgcgccac tccggtggcg ccgacaagcg cgaggctgcg gcctagacca gcggcaatcg
     3181 catctgtccg agcaccgcgc ggccaagcag gccgaacggg ttggcgagcg tttccagcat
     3241 ttcgaaatga ttgaagcctt cgccgaccaa tagctcgacc ggtttgccgg cggctttcac
     3301 ggcggtggcg aaatcacggg tctggcgctg gaattccggc gtctcgtagg tgccgtagct
     3361 caggatcagc ggcgtgtgca accggtcgag atggcgcatc gcggaaagct cgtgttccat
     3421 ctcatcggtg aacttgacgt atttggagcg cttcgacagg cgcaccggtt tgagatcgta
     3481 catgccgctc gatagcgtcg cgcccttgag aatgtcgagc ggcagtccgt ccttcgccca
     3541 atcgtgcgtc accgtacagc cggagagatg cgagcccgat gaatgcgaga cgagatgcaa
     3601 ccggttgcga tcgccgccaa atttctcggc gttgcgatag acccagccga ccgcccgccg
     3661 cacctgctcg accatcggaa acag
//
LOCUS       DASZMC010000645         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2471947,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000645 DASZMC010000000
VERSION     DASZMC010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471947"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VG274_13110"
     CDS             <1..278
                     /locus_tag="VG274_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07645.1"
                     /translation="GIFMSEFLVVTSTFARSPVLAIPLVIGLLIAIGALFLRLGGLIF
                     GEPRGRSDPDNASYAPFLAHLALVLAAGIWLPGPIVSWFQNVAHLLG"
     gene            282..1913
                     /locus_tag="VG274_13115"
     CDS             282..1913
                     /locus_tag="VG274_13115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWE07646.1"
                     /translation="MSILGNLTDTERVESHRPWPRVVVNESGWRLTAGQLAKGERELV
                     ALWGEKDSVHLAIRCPAANEFAVLSLVCVDGTYPSLSQFHPPALRLERAIRDLYGLVP
                     EGLPDERPWLDHGRWGIKHPLAIQPNVSWPPEERATQVAPLGVFDHRADARWLGGPAK
                     PGHDNSLVSNSQSSTTNYAFLPATGDSLHQVAVGPVHAGIIEPGFFQFTASGEIVVRL
                     EERLGYVHKGIESLMTGADLEHGTRLAGRVSGDSTVAYAFAFARAVEAALGIEAPPRA
                     QWLRALMGEIERIANHLGDFGAICNDASFAIMQAQCAILRERVLRAAGACFGHRLMRD
                     CIRIGGVAVDLSADAIAALSRLAMEIGLRLPRLTELYDKTASLQDRTVGAGFLSSKLA
                     RQYACGGFVGRASGREFDARKAIAYPPFDRIAFEAPVLEEGDVNARVWIRIREIEQSV
                     SIVRQILDRLPAGPLRIEFEIRPGEGVALVEGFRGDIFASVRIGENGAIERCHLRDQS
                     WFQWPLLEAASEGNIVADFPLCNKSFNCSYSGHDL"
     gene            1917..2435
                     /gene="nuoB"
                     /locus_tag="VG274_13120"
     CDS             1917..2435
                     /gene="nuoB"
                     /locus_tag="VG274_13120"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HWE07647.1"
                     /translation="MRNVLLESLRGALTEAPPPPDEAMLAELARSLDERARERLGRSL
                     AIRQVDAGSCNGCELEIHALNNAFYDLERFGLSFVASPRHADVLLVTGPVTKNMREAL
                     ERTFNATPDPKWVVAAGDCALNGGMFAGSYACVGGVSNVIPVDLHIPGCPPTPTQLLQ
                     GLLALLRVSEDF"
BASE COUNT          458 a          898 c          850 g          529 t
ORIGIN      
        1 cgggcatttt catgagcgaa ttcctcgtcg tcacctccac cttcgcccgt tctcccgtgc
       61 tcgccattcc gctggtgatc ggtcttctga tcgcgatcgg tgcgctcttt ctgaggctcg
      121 gcggtttgat cttcggcgaa ccgcgcggcc ggtcggatcc ggacaacgcg tcctatgcgc
      181 cattcctcgc ccatctcgcc ctcgtgcttg ccgccggcat ctggcttccg ggaccgatcg
      241 tgagctggtt tcagaacgtg gcacatctgc tcggctgatg catgagcatc ctcggaaacc
      301 tcacagacac cgaacgggtc gagagccatc gcccttggcc gcgcgtggtc gtgaacgagt
      361 ccggctggcg cctgaccgcc ggccagctcg cgaaaggcga gcgcgaactc gtggcgctct
      421 ggggcgaaaa agactccgtt catctggcaa ttcgctgccc ggctgcgaac gagttcgccg
      481 ttctctcgct cgtatgcgtg gatgggacat atccttcgct ctcgcaattc catccgccgg
      541 ctttgcgcct cgaacgcgcg atccgcgatc tttacgggct cgtgcctgag gggctgccgg
      601 atgagcgtcc ctggctcgat catggccgct ggggcataaa gcatccgctg gctatacaac
      661 caaacgtgtc atggcccccc gaagagcggg ccacccaggt ggcaccgctc ggagtgtttg
      721 atcaccgcgc ggatgcgcgc tggctgggtg gcccggctaa accgggccat gacaattcgt
      781 tggtatccaa ttctcaaagt tcgaccacga actatgcgtt tcttcccgcg acaggcgata
      841 gccttcacca ggtcgcagta ggaccggtgc atgcgggaat catcgagccg ggctttttcc
      901 aattcaccgc aagcggcgaa atcgtcgtgc gtctcgaaga aaggctcggt tacgttcaca
      961 agggcatcga gagcctgatg accggcgccg atctcgagca tggcaccagg ctcgccggac
     1021 gcgtttcggg cgacagcacc gttgcatatg ctttcgcatt cgcgagggcc gtggaagcag
     1081 cgctcgggat cgaggcgccg ccgcgtgcgc aatggcttcg cgcgctgatg ggcgaaatcg
     1141 agcgcatcgc caaccatctg ggcgatttcg gcgcgatctg caacgacgcc tccttcgcca
     1201 tcatgcaggc gcaatgcgcc atccttcgcg agcgcgtgct gcgcgccgcc ggcgcctgtt
     1261 tcggccatcg cctcatgcgc gactgtatcc gcatcggcgg ggtcgcggtg gacctttcgg
     1321 cggacgcgat cgcagcgctc tcgcgcctgg cgatggaaat cggtctcagg cttccccggc
     1381 tgaccgagct ttacgacaag acggcctcgc ttcaggaccg caccgtcggc gcgggatttc
     1441 tctcgtcgaa gctggcgcgg caatatgcgt gcggcggatt cgtcggccgc gcctccggac
     1501 gcgaattcga tgcgcgcaag gctatcgcct atccgccctt cgaccggatt gcattcgagg
     1561 cgccggtgct cgaggaaggc gacgtgaacg cccgcgtctg gatacgcatt cgcgaaattg
     1621 agcaaagcgt gtcgatcgtc cggcaaattc tcgatcgcct acccgccggc ccgctccgaa
     1681 tcgaatttga aatccggccg ggagagggtg tggcgctggt cgagggattt cgcggcgaca
     1741 tcttcgcttc ggtgcggatt ggagagaatg gcgcgatcga acgctgccat ctgcgcgatc
     1801 agtcgtggtt tcagtggccg ctgctcgaag cggccagcga gggcaacatc gtggcggact
     1861 ttccgctctg caacaaatct ttcaactgct cgtattcggg acacgatctg tgacgcatgc
     1921 gcaacgttct tcttgaaagc ctgcgcggcg cgctgacgga agcgcctcct ccgcccgacg
     1981 aggcgatgct tgccgagctt gcgcgctcac tcgacgagcg cgcgcgggag cgactcggcc
     2041 gcagcctcgc catccggcag gtcgatgccg gttcctgcaa cggctgcgag ctggaaatcc
     2101 acgcgctcaa caacgccttc tacgatctgg agcgcttcgg cctgagtttt gtcgcctcgc
     2161 cgcgtcacgc cgatgtgctg ctggtgaccg gaccggtgac gaagaacatg cgcgaggcgc
     2221 tggaacgcac gttcaacgcg acgccggatc ccaaatgggt ggtcgcagcg ggcgattgcg
     2281 cactgaatgg cggcatgttc gcgggaagct atgcctgcgt cggcggcgtt tcgaacgtaa
     2341 ttccagtcga tctccacatc cccggctgcc caccgacgcc cacgcaattg ctgcaaggtc
     2401 tcctcgccct cctgcgcgtt tcggaagatt tttaacgcga tggccgcaga ggatgcgcgg
     2461 aggacgcaga ggaatttcat gcgcgcttcg cgcgcagtcc tcttcctgcg tcccctgcgc
     2521 atcctctgcg gccaccgcgt taaatcttat tccggtgaca cctctcattg actgcaagca
     2581 ttggcgcgga cgaactatgt ccccataaca cgcgcggcgc cgaggcccct cgccacgagc
     2641 acgccgtaga ccgccaccca cacatagcag agcaccggca cgaagagcgc ggtcgccagg
     2701 ccccttgcat cggcgacggc gcccgtggcg agggg
//
LOCUS       DASZMC010000646         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1903425,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000646 DASZMC010000000
VERSION     DASZMC010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903425"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1367)
                     /locus_tag="VG274_13125"
     CDS             complement(174..1367)
                     /locus_tag="VG274_13125"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWE07648.1"
                     /translation="MTSDETPSRIEPCLLEETSAEIVDLVAALSAATGTLGARLHARS
                     AAGLADLVRVMNCYYSNLIEGHNTTPREIVRALEDRFDTVRERRNLQLEARAHIRVQR
                     EIDRQHAAGELPEPAAAVFIRGLHRAFYDDVPAEMLVIQGRNRQVTMTPGLFRASPEH
                     DVTVGRHLPPSSDCVEAFMAHFERRYRFEPLGMGMRITAMAAAHHRLNYIHPFLDGNG
                     RVSRLMSHAMALKAGIGAHGLWSVSRGLARGLESRGDYKRMMDHADMPRQGDLDGRGN
                     LSRRALSEFTVWFLKVCLDQVTFMTGLFALDTLVDRLKAYVARRALKPGAFVILEQVL
                     QRGELPRGEAGRVSGLKERSARDLLATLVQDGILGSDTPKGHVSLRVPLEAVEVLFPN
                     LFPET"
     gene            complement(1730..1942)
                     /locus_tag="VG274_13130"
     CDS             complement(1730..1942)
                     /locus_tag="VG274_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07649.1"
                     /translation="MGYVEALERFSELEDSYPEEAVLRNPAVFFIAKTFGKTLDKVAA
                     DINRRYLKSYRRSRCRIFNLLPPDWD"
BASE COUNT          400 a          715 c          679 g          436 t
ORIGIN      
        1 gcgcgtcgga ccgttcatca ttgcgcgccg ctacacgtag ctgcccatgt cgctgaaatc
       61 ggcgcgcagg aaaacgtcgc cgtgcttgcg ggcgatgcgc ttcacgatgc gatcctccaa
      121 tgtctctccc gtcacggcaa atttctcccc cttcgccttg cccgcccgcg gtgtcaggtc
      181 tcagggaaca ggttcggaaa gagaacttcg acagcctcca gcgggacgcg cagcgacacg
      241 tgaccctttg gcgtgtcgga gccgagaatg ccgtcctgaa ccagcgtcgc aagcagatca
      301 cgcgccgacc gctccttgag gccgctcacg cgcccggcct cgccgcgcgg cagttcgccg
      361 cgttgcagca cctgttcgag gataacgaat gccccgggct tcagcgcgcg gcgggcgaca
      421 taggctttga ggcgatcgac cagggtgtcg agcgcgaaca ggccggtcat gaaagtgacc
      481 tggtcgagac agaccttcag gaaccagacg gtgaactcgc tcagtgcccg gcgcgacagg
      541 ttgccgcgcc cgtcgagatc gccctgccgc ggcatgtcgg catggtccat catccgcttg
      601 tagtcgccgc ggctttcgag accgcgcgcc agcccgcgcg agaccgacca caacccgtgc
      661 gcgccgatcc ctgccttgag cgccatggca tggctcatca ggcggctcac ccgcccgttc
      721 ccgtcgagga agggatggat gtagttcagc cgatgatgcg ccgccgccat ggcggtgatg
      781 cgcatgccca tgccgagcgg ctcgaaccgg tagcgccgct cgaaatgcgc catgaacgct
      841 tcaacgcagt cggaggacgg cggcaaatgg cgtccgactg taacgtcatg ctccggcgat
      901 gcccggaaca agccgggcgt catcgtgact tgccggtttc ttccttggat gaccagcatc
      961 tccgcgggaa cgtcgtcgta gaacgcccgg tgcaatccgc ggatgaagac ggctgcggcc
     1021 ggttccggca gttcgccggc ggcgtgctgg cggtcgattt cgcgttggac ccggatatgc
     1081 gcgcgggctt cgagctgaag attgcggcgc tcgcggacgg tatcgaaccg gtcctccagc
     1141 gcccggacaa tctcgcgcgg cgtggtgttg tggccctcga tcaggttcga atagtagcag
     1201 ttcatgacgc gtacgagatc tgccagtcct gcagccgagc gggcatgcag ccgcgcgccg
     1261 agcgtgccgg tcgcggcgga cagcgcggcg accaggtcga cgatctcggc gctcgtctct
     1321 tccagcaggc agggttcaat ccgcgagggg gtttcgtcgc tggtcatcac tgccgatcat
     1381 gaaaatggga tatccaaagg tgatataaca taactcctga ttttgtacca cgattttgcc
     1441 gatctttctt ccgatcctct gtcgcggcta tgtgcctcag attacgggtg tttcaccagg
     1501 tttgtgcgct aaatctgccg atcctggcgc tcttcagcgg ggcaatccgc gcttcgcaga
     1561 ggggccgtcg cttccgccgt tcgatgcgtg actccaatgc ctgcaaattc cggacatcca
     1621 tcgacgccac ccgccgatga tgcagatcac aggtggcgga ccaccaacaa gcagaaggcc
     1681 gtctggactt gtgcctcaac gacaaaaccg cgcccccttg cgcagacgct caatcccaat
     1741 ccggtggaag gaggttgaag attcggcagc gcgatctcct gtaactcttc aggtatcgcc
     1801 ggttgatatc ggcggcaact ttatccagcg ttttgccgaa ggtcttcgcg atgaaaaaca
     1861 cggctgggtt tcgaaggacg gcttcttcgg gatagctgtc ttccaactcg ctgaaccgtt
     1921 caagagcttc cacatatccc acgcctggcc ttgccttctt tggctttgat cgcggctttg
     1981 gtcgcttttg catgacccga atataattcg ctcttcgata ccgtacaacc gaatccgccg
     2041 gtgcgccctg ccacgcgatc acgcgtgaca ggtcaggctc tactcgggcg aaaccgccct
     2101 ccccgagccg caaagatgcg tctcggcgca atggcgtgac atggggtaac agcggcgccc
     2161 caccgaccgt ttgatccggt tcagggatgg gtgtcggctg ctccggcttt cacagagagg
     2221 agcactccat
//
LOCUS       DASZMC010000647         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3824485,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000647 DASZMC010000000
VERSION     DASZMC010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3824485"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VG274_13135"
     CDS             complement(<1..416)
                     /locus_tag="VG274_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWE07650.1"
                     /translation="MTRIISWAVANTRLVIAALVVTIIAGVIAFIAIPKESDPDIPIP
                     YIVVEVYYPGISPEDSERLLVKPMENYLRSIQGLKEMTARAYEGAAVVIMEFDVSFNK
                     DRALADVRAQVDTARAELPADVQQPIVQEISTSLFPV"
     gene            complement(413..1546)
                     /locus_tag="VG274_13140"
     CDS             complement(413..1546)
                     /locus_tag="VG274_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWE07651.1"
                     /translation="MSGNLLIALRRFWDRIQPGFWRRMKPSYRAATAITILVVLWIGS
                     GVLSFGGTKQSDDAQAKSAQMPSVQVERFAAQNRDASITVRGRTQALHSVDVKAEVDG
                     VVQALHFEKGDFVRAGQVLCDLKTNDRAAKLDQARAQVAETDQKYKANIALDKAGYLA
                     KTLLAESATALESARAIERTMEIDLANTQIRAPFDGLVDDRYVNVGDYMAVADKCALL
                     VAPQPFLAIGMVSEHDVSSIRPGDPASATLVTGETVQGHVRFIGERADTNTRTFKLEV
                     DLPNPDAKLRDGVSADIHIPVRNLKAAKISPGILVLDDNGVVGVRTVENGVVHFKPVQ
                     IVSDGPDGMWVTGIADGAVVITVGQEFVSEGSRVKSVLSKARA"
     gene            complement(1557..2087)
                     /locus_tag="VG274_13145"
     CDS             complement(1557..2087)
                     /locus_tag="VG274_13145"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWE07652.1"
                     /translation="MNVRTICLGLLTRGDATGYEIKKQFEEGSYRHFAEASFGSIYPA
                     LGRLTAEGLVLVREEAQDKRPDRKVYSITSAGRESFVGALLEPLKDDRHRSPFAFAML
                     FSDLLPEDRTCGLIKAYLADKEAALAQMNARKSAAQTRGERFVNGLAKSIHTAAIGYL
                     RAHRGEFEACAREAAE"
     gene            complement(2261..>2496)
                     /locus_tag="VG274_13150"
     CDS             complement(2261..>2496)
                     /locus_tag="VG274_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWE07653.1"
                     /translation="RERDHGPPGPMIEGKVKFFNDQKGFGFVMPENGGADIYIHASAL
                     RRSGLTALSPEQRIRFSTRQGTKGVEVDRIEAV"
BASE COUNT          434 a          783 c          791 g          488 t
ORIGIN      
        1 accgggaaca ggctcgtgct gatttcctgg acgatcggct gctgcacgtc ggccggcagt
       61 tcggcgcgcg ccgtatcgac ctgcgcgcgc acgtcggcga gcgcccggtc cttgttgaag
      121 gagacgtcga attccatgat gacgacggcc gcgccctcat aggcacgcgc cgtcatctct
      181 ttcagcccct ggatggagcg gagataattc tccatcggct tgacgagaag ccgttcgctg
      241 tcctccgggc tgatgccggg gtagtagacc tccaccacga tgtaggggat cggtatgtcg
      301 ggatccgatt ccttcgggat ggcgatgaat gcgatgacgc ccgcaatgat ggtgacgacc
      361 agcgcggcga tgaccagccg cgtgttggcg acggcccagg agatgatccg cgtcatgcgc
      421 gcgccttcga cagcaccgat ttgacccgcg acccctcgct cacgaattcc tggcccaccg
      481 tgatgacgac cgcgccgtcc gcgatcccgg tcacccacat tccgtcgggt ccgtccgaca
      541 cgatctgcac gggcttgaag tgcaccacgc cattttcgac cgtgcgcacg cccaccacgc
      601 cattgtcgtc gagcaccaga atcccgggcg atattttcgc cgccttgagg ttgcggacgg
      661 gaatgtgaat gtcggcgctc accccgtcgc gcagtttcgc gtcgggattg ggcagatcga
      721 cttcaagttt gaaggtgcgg gtattcgtgt cggcgcgttc gccgatgaag cggacatggc
      781 cctggacggt ctcgccggtg acaagcgtgg cgctcgccgg atctcccggc ctgatgctgc
      841 tcacatcgtg ttcggaaacc atgccgatgg cgaggaacgg ttggggcgcg accaggagcg
      901 cgcatttgtc ggcaacggcc atatagtcgc cgacattcac gtagcggtca tcgacgaggc
      961 cgtcgaaggg cgcgcggatt tgcgtattgg cgagatcgat ttccatcgtg cgttcgatcg
     1021 cacgcgccga ttcgagggcg gttgcggatt ccgccaacag cgtctttgca aggtagccgg
     1081 ccttgtcgag agcgatattc gccttgtact tctggtcggt ctcggccacc tgcgcacgtg
     1141 cctggtcgag ttttgccgcg cggtcgttgg tcttgagatc gcacagcacc tgaccggccc
     1201 tgacgaaatc gcccttctcg aaatgcagcg cctgcacgac gccgtccact tccgccttca
     1261 cgtcgacgga gtgcagcgcc tgggtgcggc cgcgcacggt gatggaggca tcgcggtttt
     1321 gcgcggcgaa gcgctccacc tgcacgctcg gcatctgggc ggacttggct tgcgcgtcgt
     1381 cggactgctt cgtgccgcca aaggaaagca cgcccgaccc gatccacagc acgacaagaa
     1441 tcgtgatcgc cgtcgctgcg cggtagctcg gcttcatgcg ccgccagaag ccaggctgaa
     1501 tgcggtccca gaaccggcga agcgcgatca acaaatttcc cgacatttcg actttcctat
     1561 tccgccgctt cccgggcgca tgcttcgaat tcgccgcgat gggcacgcag atagccgatt
     1621 gccgcggtgt ggatcgactt ggccaagccg ttgacgaacc tctcgccccg ggtttgcgcc
     1681 gcagatttgc gggcattcat ctgtgcaaga gccgcttcct tgtcggccag ataggccttg
     1741 atgaggccgc aggtgcgatc ttcgggcaga aggtccgaaa aaagcattgc gaacgcaaaa
     1801 ggcgagcggt ggcggtcgtc cttgaggggt tccaacaagg cccccacgaa gctttcccgg
     1861 ccggccgaag tgattgaata aaccttgcgg tccgggcgct tgtcctgcgc ctcttcgcgg
     1921 accaggacca ggccttcagc ggtcaggcgg cccagcgcag gatagatcga cccgaagctg
     1981 gcttcggcaa aatgccgata gctgccctcc tcgaactgct tcttgatttc gtagccggtg
     2041 gcgtcgccgc gcgtcagcag ccccagacag atcgtgcgaa cgttcatttg gcgcgccccg
     2101 acgcggctat gctggttcga tatagctgcg ggctatatat ttttgtagcc gtcgaatgca
     2161 aggccggatg cacgcaaatc ggggcgcgcg cagcgcacag ctggcctgcc cggctgcgag
     2221 gggcaaaaac tagcggatga ggtgcgctct tgagacgccg ctaaaccgct tcgatgcgat
     2281 cgacctcaac ccccttcgtg ccctgccggg tggaaaagcg gatgcgttgt tcgggggaaa
     2341 gcgccgtcag gcccgagcgc ctgagcgcgc tcgcatgaat atagatgtcg gcgccgccat
     2401 tctcgggcat cacgaagccg aagcctttct gatcgttgaa gaatttgacc ttgccctcga
     2461 tcatgggccc cggtggaccg tggtcgcgct cgcgat
//
LOCUS       DASZMC010000648         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3563685,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000648 DASZMC010000000
VERSION     DASZMC010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3563685"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..699
                     /locus_tag="VG274_13155"
     CDS             262..699
                     /locus_tag="VG274_13155"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWE07654.1"
                     /translation="MARSERADSASGAAEKSANKLPNQHSRREREIMDALYRLGKASA
                     AEIREQMPDPPTYTAIRTHLSILEKKGHVRHESDGTRYIYEPLVAREQMGRRAIDSLL
                     TTFFDNSVERAVTALLTRQDANIPRADLDRLARLIDKARKEGR"
     gene            696..2090
                     /locus_tag="VG274_13160"
     CDS             696..2090
                     /locus_tag="VG274_13160"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HWE07655.1"
                     /translation="MTELIALRALLFAGELLAGSAVILSFAWGLAAQRSASARHLTWA
                     GAFGALLALPVLMAIFPSAFHLLLPAPPRLPLVQTFDVVEMAALPAPPPGGFSFDAPN
                     IALALGTLWIAGVCAAAARLTVAAFCLSALRRRSRLFALPPQALPKVTATHRECELRL
                     SERDVGPIAWGLFRPVILLPESATVWPRDRLHSALLHELAHIRRRDWAVQALAHLVCA
                     LYWPNPLVWIGAKNLRREAEIAADDAVIVSGVRPSSYAGELLELAAEFRVRRPLALAN
                     MALFMAELSALEARVESVLAPTSLRTGVTSMDVVKIASAGLFAAAAIAFACPSLAQDA
                     QTAPVTTAPLAPSPPDAPPPPGAPRAGGATGSARASRSGGARGFCCSRHATYSRTSGF
                     TRTAEAETYRPETPAPRSRKSKARGDQASQARNQTSRPRSACERGCDARRGSSAARHR
                     PSYGKCHEGSAGNR"
     gene            2053..2334
                     /locus_tag="VG274_13165"
     CDS             2053..2334
                     /locus_tag="VG274_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07656.1"
                     /translation="MESVTRVRPEIDKAWAEARPEVDRTLADARAELAKAHVDAKIQA
                     RVDAALKRAEIGIEAAESRDHDGESRHEERSEESDGPDSPDNPDMPDEN"
     gene            2374..2760
                     /locus_tag="VG274_13170"
     CDS             2374..2760
                     /locus_tag="VG274_13170"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HWE07657.1"
                     /translation="MSSRGFRAVYAALDPATAILEVAVHKTFKTLDTVPHVLISAWIA
                     DAKHVRIVKPADVPNPNWLLPGAVSAGQQAFGDALLKGHPFALIPSVVSRRSWNVIFD
                     PALAEGLYSSIAEEPFALDTRLHRPS"
     gene            2869..>3360
                     /locus_tag="VG274_13175"
     CDS             2869..>3360
                     /locus_tag="VG274_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07658.1"
                     /translation="MTLTTIAMLAGVAVSLGLLVILVLRKPLADAGTGTIAEQLQTIK
                     AVVERLDRVVREDGEMARAGSDERGRLLRNEVSKTLADSRAEMSTTMAAVRAEIVSAL
                     EKAGHDLRTSSAELSKVQKERLDDVGAKLFLLTDALEKRLESNRTMLETKFGEIRNES
                     TSAS"
BASE COUNT          630 a         1076 c         1029 g          625 t
ORIGIN      
        1 cgagttcctg aaatagcgtg tcatccccgc ccagccctcg cgaaagcggg ggcgagagcc
       61 ggacaggaaa catgtgcggc gcgtatctga tttacgatac tgggtccccg atgacgcgct
      121 ggcgcgcgtc tcggggatac cacgcttggg aacgaggtcg cgcggcgaac atcgagcgcg
      181 ccgcatgcaa aaatattcac aaacgcgcga agcccataga tagcgattga cagctctcaa
      241 aatgtatgcc aaatttttgg catggcgaga agcgagcgag cggacagcgc ttccggggcc
      301 gcggagaaat ccgcgaacaa gctgcccaac cagcacagcc gccgcgagcg cgagatcatg
      361 gacgcgctct acaggctcgg caaagcctcc gccgcggaaa tccgcgagca gatgcccgat
      421 ccgccgacct acactgccat ccggacgcat ctctcgattc tcgagaagaa aggccatgtg
      481 cgccacgaga gcgatgggac gcgctacatc tacgagccgc tcgtcgcgcg cgagcagatg
      541 ggccgccgcg ccatcgattc gcttctcaca acattcttcg acaattccgt ggaacgcgcc
      601 gtcaccgcgt tgctgacgcg ccaggatgcg aacatcccgc gcgcggatct cgaccggctg
      661 gcgcgcctca tcgacaaggc caggaaggag ggacgatgac cgagctcatt gcattgcgcg
      721 cgcttctctt tgcgggcgaa ttgcttgcgg gatcggctgt gatcctgtca tttgcctggg
      781 gtctggccgc gcagaggtcg gccagcgcgc gccatctcac ctgggcgggt gccttcggtg
      841 cgctgctcgc cttgccggtg cttatggcga ttttcccctc cgcgtttcat ctcctgcttc
      901 ccgcgccgcc gcgcctgccg cttgtgcaaa ccttcgacgt cgtcgagatg gcggcgctcc
      961 cggcgccgcc gccaggcggt ttctctttcg acgcgcccaa tattgcgctt gctctgggca
     1021 cgctctggat cgcgggcgtg tgcgcggccg cggcgcgcct caccgttgct gcgttctgcc
     1081 tctccgcgct cagacgccgc agccgtctct ttgccttgcc tccgcaggct ctgcccaaag
     1141 tgaccgcgac ccatcgcgaa tgcgaattgc gcctctcgga gagggatgtc ggtccgatcg
     1201 cctggggcct tttccgtccg gtcatcctgc tgccggagag cgcgaccgtc tggccgcgcg
     1261 acaggctgca ttccgctctg ctgcacgagc tcgcccacat tcgacggcgc gattgggctg
     1321 tccaggcgct ggcgcatctg gtctgcgcgc tttactggcc caatccgctt gtctggatcg
     1381 gcgcgaaaaa tctgcggcgc gaagccgaga tcgccgcaga cgatgcggtg atcgtctcgg
     1441 gcgtgcggcc atcttcctat gccggcgaat tgctcgagct tgcggcggaa ttccgggtgc
     1501 ggcgtccact ggcgctcgcg aacatggcct tgttcatggc cgagctctcg gcgctcgaag
     1561 cgcgcgtcga atcggttctc gcgccaactt cactgcgcac gggagtgaca tcaatggatg
     1621 tcgtaaaaat cgcaagcgcc ggactttttg ccgccgcggc aatcgcgttt gcctgtccct
     1681 cgctcgcgca ggatgcgcag accgcaccgg taactaccgc gcccctcgcg ccatctccgc
     1741 ccgatgcacc gcctcctccc ggtgctccac gcgccggagg cgccaccggt tccgcccgcg
     1801 cctcccgctc cggtggcgcc cgtggatttt gctgctcccg ccatgcaaca tattcacgta
     1861 catctggatt cacgcgaact gcagaagctg aaacatatcg acctgaaacg cctgcacctc
     1921 gcagtcgaaa gagcaaagcg cgaggcgatc aggcgagcca ggcccgaaat caaacgagcc
     1981 gtccgcgaag cgcatgtgag cgaggatgcg atgcgcgccg tggaagcagc gcagcccgac
     2041 atcgaccgag ctatggaaag tgtcacgagg gttcggccgg aaatcgataa ggcatgggcc
     2101 gaggccaggc ccgaggtcga tcgcactctc gccgatgccc gcgcggaact cgccaaagct
     2161 cacgtcgatg cgaaaatcca ggcacgcgtg gatgcagcac tgaagcgcgc ggagatcggg
     2221 atcgaggccg cggaatcgcg cgaccacgat ggggaatccc gccacgaaga gcgcagcgaa
     2281 gagtcagatg ggccggacag cccggataat cccgatatgc cagacgagaa ctgatggaac
     2341 caggtccgcc gcgaagcaat tttttcggcc gaattgagtt cgcgcggatt tcgcgctgtc
     2401 tatgcggcgc tcgatccggc caccgccatc ctggaagtcg cggtgcacaa gacgttcaag
     2461 acgctcgaca ccgttcccca tgtcctcatc tccgcgtgga ttgccgacgc gaaacacgta
     2521 cgcattgtca agcctgcaga cgtgccgaac cccaactggc tgctgcccgg cgccgtcagt
     2581 gccggccaac aggcgttcgg cgacgctctg ttgaaggggc acccgtttgc actgatcccg
     2641 agcgtcgtgt cccggcgcag ctggaatgtg atcttcgatc cggcattggc tgaagggctc
     2701 tacagctcga ttgcggagga acccttcgct ctcgacacgc gcctgcaccg gccgagctga
     2761 cttgcgccct ggttgcatcg tggccagctt gccagttccc tccgtacgca acgcccaatt
     2821 ttcgagtatt gttgtcgtca gtctcgactc tgaaaggggc atttttcgat gaccttaacg
     2881 actattgcca tgctagcggg cgtcgcagtc tcgctgggcc tgttggtaat tcttgtactc
     2941 aggaaaccgc tggcagacgc aggaactggg acaattgcag agcagcttca aacgattaaa
     3001 gcagtcgttg aacgcttgga tcgggtggtc cgtgaagatg gcgagatggc ccgtgccggc
     3061 tcggacgagc gagggcgact gcttcgaaat gaagtgtcca aaacgcttgc cgattcccgc
     3121 gccgagatgt ccacgacaat ggctgccgtt cgagcggaga tcgtttctgc ccttgaaaag
     3181 gccgggcatg acttgagaac ctcgtctgcg gagctgtcca aagtgcagaa ggaaagactc
     3241 gacgacgttg gcgcaaaact ttttttgctt actgatgctc tcgagaagcg ccttgagagc
     3301 aaccgtacca tgctcgagac aaagtttgga gaaatcagga atgagtccac aagtgcgtcg
//
LOCUS       DASZMC010000649         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2741511,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000649 DASZMC010000000
VERSION     DASZMC010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741511"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..990)
                     /gene="kynA"
                     /locus_tag="VG274_13180"
     CDS             complement(163..990)
                     /gene="kynA"
                     /locus_tag="VG274_13180"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HWE07659.1"
                     /translation="MTGAAEPPEGAETDFRRRMSYSDYLKLDVLLHAQAPLSTAHDET
                     LFIIQHQTSELWMKLAIHEIRAACDAIRADRLQQTCKILARVSRIFEQLNNAWDVLRT
                     MTPSEYTEFRESLGQSSGFQSWQYRAIEFLVGNRNLAMLKPHEHDAEITGELTRILTQ
                     PSLYQEAIALLGRDGFDVGAEPSDLAASHVESEAVLQAWATIYRNPTKYWSLYELAEK
                     LIDFEDYFRRWRFNHVTTVERVIGLKRGTGGTSGVSYLRRMLDVVLFPELWKVRTTL"
     gene            complement(987..>1590)
                     /locus_tag="VG274_13185"
     CDS             complement(987..>1590)
                     /locus_tag="VG274_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWE07660.1"
                     /translation="RAGADFAVGCGYKYLNGGPGAPAFIYVRPDLQDEVAPVLSGWMG
                     HEAPFEFDLHYRPAPAIHRQRAGTPAVLSMAALEAALTVWDGVDMAVVRARSITLGEA
                     FIRTIEQLCPELELASPRDPERRGSQVSFRFAHGYEVMQALIAEGIIGDFRAPDIMRF
                     GFTPLYLSPGEVETAARRLAAVINQGLWDQPKFRARNKVT"
BASE COUNT          292 a          496 c          513 g          289 t
ORIGIN      
        1 ccgatccggc gccgaagtat atgaagctgc ggcgtggttg gacgaagtgg atggccggga
       61 caaccctggc catgacggtc tgacctgcgc gtttcacgcc tcgcgtctca tcggtcgctg
      121 tctcccaaac gccgtagcgt acacgatgtc ggcgtcagct ctctacaacg ttgtcctcac
      181 cttccacaat tccggaaaca gcacgacgtc tagcatccgc ctcaaatacg aaaccccgga
      241 agtgccgccg gtgccgcgct tcagcccgat cacgcgctcg accgtggtga cgtggttgaa
      301 gcgccagcgg cggaaatagt cttcgaagtc gatcagcttt tccgccagtt cgtagagcga
      361 ccagtatttc gtgggattgc gatagatcgt cgcccaggct tgcagcaccg cttcgctctc
      421 gacatgcgaa gccgcgaggt cagagggctc cgcgccgacg tcgaagccgt cgcggccgag
      481 cagtgcgatc gcctcctgat agaggctcgg ctgcgtcagg atgcgcgtca gttcccccgt
      541 gatctccgcg tcgtgttcgt gcggcttcag catcgcgaga ttgcggttgc cgacaaggaa
      601 ttcgatcgcc cggtattgcc aggattgaaa gccggaggac tgcccgaggc tctcgcggaa
      661 ctcagtatat tcgctcggcg tcatcgtgcg cagcacgtcc caggcgttgt tcagctgctc
      721 gaatatgcgc gaaacccgcg cgagaatctt gcacgtctgc tgcagcctgt ccgcgcggat
      781 cgcatcgcag gcggcgcgaa tttcgtggat cgccagtttc atccagagtt cggaggtctg
      841 atgctggatg atgaacagcg tctcgtcgtg tgcggtcgag agcggcgcct gcgcatgcag
      901 caacacgtcg agcttcaaat agtcggagta ggacatgcgg cgacgaaaat cggtctcggc
      961 gccttcgggc ggttccgccg caccggtcat gtcaccttgt tccgcgctcg gaatttcggc
     1021 tggtcccaga gcccttggtt gatgaccgcg gcgaggcggc gggccgccgt ttcgacctcg
     1081 ccgggactga gatagagcgg ggtgaagccg aaccgcatga tgtcgggcgc gcggaaatcg
     1141 ccaatgattc cctccgcaat cagcgcctgc atgacctcgt aaccatgggc gaaacggaac
     1201 gagacctgcg atccccgacg ttcgggatcg cgcggcgagg cgagttccag ttccgggcaa
     1261 agctgctcga tggttcggat gaaggcttcg cccaacgtga tcgagcgggc gcgaacgacg
     1321 gccatgtcca cgccatccca gacggtgagc gcagcttcca acgcggccat cgaaagcacc
     1381 gccggggtcc cggcgcgctg acgatggatc gcgggcgcgg gtcggtagtg gagatcgaat
     1441 tcgaacgggg cctcatggcc catccagccc gacaagaccg gcgcgacctc gtcctgcaga
     1501 tcgggacgca cgtagatgaa cgcgggggcg ccgggaccgc cgttgagata tttgtagccg
     1561 caacccaccg cgaaatcggc gccggcgcgg
//
LOCUS       DASZMC010000650         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3825895,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000650 DASZMC010000000
VERSION     DASZMC010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3825895"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VG274_13190"
     CDS             complement(<1..520)
                     /locus_tag="VG274_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07661.1"
                     /translation="MSNCVCISTLAAASGSGVIPSGTPAVVIEQFDTSSAVSHALYVP
                     TGLIAAPPHVLYFKNGSTYWVIHPDHVSLEAAGAPAAGPTNDCAPAMQRVLDYLATVG
                     QAKLQLWERDYVLQSCATLTQSNPTTDPLLYFTIQGRGRGVSRLMVQYVPTPANPPGA
                     FAIVFSSNQSEFV"
     gene            complement(591..1469)
                     /locus_tag="VG274_13195"
     CDS             complement(591..1469)
                     /locus_tag="VG274_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07662.1"
                     /translation="MSLLCYDYIQGKEASMSNGLRFDTLATLAGASIPDGTESVVIEQ
                     FDLCSADSAARYIPVETNPGHDLSVLNAPGNGWWAVHPDHLSLEAAGAPADISGDCAS
                     AMQRVLGYLAAATRAKLQLHERDYVLNSRAALAQSSGDPPLSLTIMGRGRDASRLLVH
                     YPDNQAGALALAFADTRSEFVARDFSVIATGATGPGGDCGTGIRIGFPESADTGAAHG
                     AVIANLFVGGEDERPNGAGDSFFSKPIDIAGASRPLVSGCRIGAPAGPDAMAGEPGEL
                     GPLPGSADATPEGRGL"
     gene            complement(1571..1825)
                     /locus_tag="VG274_13200"
     CDS             complement(1571..1825)
                     /locus_tag="VG274_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07663.1"
                     /translation="MTELLRNRQANPGQYSILIQARHSGPDAGIQGQRPALLPVLIRV
                     AAPMRIENESAGELLCIHSAAADGRRLHLGSCFCGNDIFT"
     gene            2014..3417
                     /locus_tag="VG274_13205"
     CDS             2014..3417
                     /locus_tag="VG274_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWE07664.1"
                     /translation="MESVNPFTGKAATHYDEMTRDQVDSAIDSSHRAYRDWRRRAYAE
                     RAEPMRRAAVLLRERAGEYAKLMAVEMGKPVADGIAEARKCAAGCDFYAENAERFLAA
                     QPVDLEDRKARVVFAPLGVVLAVMPWNFPFWQVIRFAAPALMAGNAAVLKHAGNVPGC
                     ALALECLFQDAGFPQHLFRTLLIGSAMVGEVIEHPHVRAVTLTGSAGAGRAVAAKAGQ
                     CLKKTVLELGGSDAYVVLADADIAFAAAQCARARLVNAGQSCIAAKRFIVIESVMAEF
                     EKQLVMEMGKARMGDPQDENTTMGPLARHDLRDALHLQVRKTIDMGARCLIGGAIPAD
                     AGAFYPPTVLGGVRKGTPAHDEEMFGPVAAVIGARDENEAFHLANDSAFGLGGAVFTR
                     DLVRGEAIARDEIEAGLVFVNAPVESNPKLPFGGIKESGYGRELSSFGIQEFVNIKTV
                     VVAPHTAARSPVASLSE"
     gene            complement(3461..>4187)
                     /locus_tag="VG274_13210"
     CDS             complement(3461..>4187)
                     /locus_tag="VG274_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWE07665.1"
                     /translation="ALTGPEAGITRDSVAAAFAAEGRTVLLHDTAGLRKRARAAGHAL
                     EQLAIASALTAIRFAECVVVVIDATQPFEKQDLTIADLVAREGRAIIFAANKWDLIER
                     KAGAIGVLRRKLDRLLPQIAGAPLVAISAMTGEGLPRLMPGVLAADGAWNKHVATAEL
                     NRLLARAVERHPPPAVKGRRVRIRYMTQPKSRPPTFALFGNQLDSLPQSWLRYLQNEL
                     REAFDFEGTPLRFALRTSKNPYA"
BASE COUNT          774 a         1281 c         1373 g          759 t
ORIGIN      
        1 cgacgaattc cgactggttg ctggagaaga cgatcgcgaa ggcgcccggc ggattggccg
       61 gggtgggaac gtattgcacc atcagccgcg acacgccgcg gccgcggccc tgaatggtga
      121 agtagagcag cggatcggtg gtcggattcg actgggtcag cgtcgcgcac gattggagga
      181 catagtcgcg ctcccacaat tgcaatttgg cctgccccac cgtcgccagg tagtcgagca
      241 ccctttgcat cgcgggtgcg caatcgttgg tcgggccggc cgcaggcgca cccgccgctt
      301 cgaggctcac atgatcgggg tggatcaccc agtaggtgga tccgttcttg aaatagagca
      361 cgtggggcgg ggcggcgatg agcccggtgg ggacgtataa cgcgtgggac accgcgctgc
      421 tcgtgtcgaa ctgttcgatg acgaccgccg gcgtcccgga cgggatgact ccggacccgc
      481 tcgcggcggc aagcgtcgaa atgcacacgc aattgctcat tttagcctcc tgagttcagt
      541 gctgcgatcg tgttgtgtca aggcgatccc ggcgggaacc gcctgtggat tcataagcca
      601 cgcccttccg gcgttgcgtc tgcgctgccg ggcaggggcc ccagctcgcc aggttcgcct
      661 gccatcgcat cgggtccggc gggagcgcca atccggcaac cgctgaccag cggacggctt
      721 gcacctgcga tgtcgatggg tttggagaag aaggagtcgc cggcgccgtt gggccgttcg
      781 tcctcgccgc ccacgaacag gttggcaatc accgcgccgt gcgccgctcc cgtatccgcc
      841 gattcgggaa atccaatgcg aatgccggtt ccacagtcgc cgccgggccc tgtcgcgccc
      901 gtcgcgatga cggagaagtc gcgggcaacg aactccgacc gggtgtcggc gaacgcaagc
      961 gccaaggcgc ccgcctggtt gtccggatag tgcacgagca gccgtgaggc gtcgcggccg
     1021 cgccccatga tggtcaagct caatggcgga tcgccgctcg actgcgccag cgccgcgcgc
     1081 gagttgagga cataatcgcg ttcgtgcaat tgcaacttcg cccgggttgc ggccgcgagg
     1141 tagccgagca cacgctgcat cgcactcgcg caatcgccgg agatgtccgc cggcgcccct
     1201 gccgcttcca gcgagagatg gtcgggatgc accgcccacc agccgttgcc gggagcattg
     1261 aggaccgaaa gatcgtgacc cggattggtc tcaaccggaa tgtagcgcgc cgccgagtcg
     1321 gcactgcaga ggtcgaattg ttcgatcaca accgactcgg tgccgtctgg tatcgacgcg
     1381 ccggcgagcg ttgcgagcgt gtcgaagcgt aagccattgc tcatggaggc ctcttttccc
     1441 tgaatgtagt cgtaacagag cagcgacatg aaactgctcc gacacagaca ctgcacacac
     1501 accgaaaccg aaaactggtt gacgcgcgat ttacctggcg ccgcgcgata tttcgttttc
     1561 tgattctcca ttaagtaaaa atgtcattcc cgcaaaagca ggaacccaaa tgcaaccgac
     1621 ggccgtccgc cgccgcacta tggatgcaaa gcagttcgcc ggcggattcg ttctcgatgc
     1681 gcatgggcgc ggccacgcga atcaaaacgg gcagcaacgc cgggcgttgg ccctggattc
     1741 ccgcgtccgg cccggaatga cgtgcctgga ttaatatgga atattgtcca gggttcgcct
     1801 gacggtttcg cagaagttcc gtcatgacac gtaacctgaa cttaatggca gcctgccgcc
     1861 agcgtccgaa tggcagcatg cccggacgaa attgaagtgg gccttgagcc gagttacgtg
     1921 gtacaggccg tggcctctac ggatacgtca tgaattttgc tggcgcgccc aaagagacaa
     1981 cgttggtgcg cttaggcagg aggacgcatc agaatggaat ccgtgaatcc gttcaccggc
     2041 aaagcagcca cgcattacga cgaaatgacg cgcgatcagg tggattcggc gattgattct
     2101 tctcaccgtg cttaccgcga ttggcgccgg cgcgcctacg ccgagcgcgc cgaacccatg
     2161 cgccgggccg cggttctgct gcgcgagcgg gccggggaat acgcaaagct gatggccgtg
     2221 gagatgggca aacccgtcgc ggacggaatc gccgaagcgc ggaaatgcgc cgccggttgc
     2281 gacttctacg ccgaaaatgc cgagcgattc cttgccgccc agcccgttga tctcgaagac
     2341 cgcaaggcgc gcgtggtatt cgcaccgctg ggcgtcgtgt tggcggtcat gccatggaat
     2401 tttccttttt ggcaggtcat ccgattcgcg gcgccggcac tcatggccgg aaatgccgcg
     2461 gtgttgaaac atgctggaaa tgtcccgggc tgcgcgctcg cgcttgagtg cctgttccag
     2521 gatgcgggct tcccgcaaca tctcttcagg acgcttctca tcggcagcgc catggtgggc
     2581 gaggtgatcg aacaccctca cgtccgggct gtcacgctta caggcagcgc cggcgccggc
     2641 cgcgccgtcg ccgcaaaggc cggtcaatgc ctcaagaaga cggtgctcga actcggcgga
     2701 agcgatgcct acgtcgtgct cgccgatgct gacattgcgt tcgcggccgc acaatgcgcg
     2761 cgggcgcggc tggtcaatgc gggtcagagc tgcattgccg caaagcggtt catcgtcatc
     2821 gagtccgtaa tggccgaatt cgagaagcag ctcgtcatgg agatgggcaa agcacggatg
     2881 ggcgatccgc aggatgaaaa caccacgatg gggcccttgg cacggcatga cctgcgcgat
     2941 gcacttcatc tccaggtgag gaaaaccatc gacatgggcg cgcgctgcct gatcgggggc
     3001 gccatccccg cagatgcggg cgccttttat cccccgaccg tgctcggcgg ggtccgcaag
     3061 ggcacgcccg cccatgacga ggagatgttc ggccctgttg cggcggttat tggggcacgg
     3121 gacgagaacg aggcgttcca tctcgccaat gattcggcgt tcggactggg cggagccgtc
     3181 ttcacgcgcg atctggtgcg tggcgaagcg attgcacgcg atgaaatcga agcaggcctc
     3241 gtcttcgtca atgcgccggt cgagtcgaac ccgaagcttc catttggcgg catcaaggaa
     3301 agcgggtatg gccgcgaact gtcgagcttc ggaatccagg aattcgtaaa catcaagacg
     3361 gtcgtcgtcg cgccgcacac tgctgcgcga tcacctgtgg cgtcgctttc tgaatagagc
     3421 ggtccccggc aaaggcggac tgcgcattgc gcctcgcgtg ctacgcataa ggatttttgg
     3481 acgtgcgaag cgcgaagcgc agcggtgtac cttcgaaatc gaacgcctcg cgcagctcgt
     3541 tctgcaaata acgcagccac gattgcggta gcgaatcgag ctgattgccg aatagcgcaa
     3601 aggtgggcgg acgggatttc ggctgcgtca tatagcggat tcgcacccgg cggcctttga
     3661 cggccggagg cggatggcgt tcgaccgcgc gcgccaggag ccggttgagc tcggccgtcg
     3721 cgacatgttt gttccaggct ccatccgctg cgagaacgcc cggcatcagg cgcggtaatc
     3781 cttcgcccgt catggcggaa atggcgacca ggggtgcgcc cgcgatctgc ggcagcaggc
     3841 ggtcgagctt tcgcctgaga acgccgatgg cgccggcttt tctctcgatg aggtcccatt
     3901 tgtttgcggc gaatatgatc gcgcgaccct cgcgcgccac gagatcggct atcgtcaaat
     3961 cctgtttttc aaagggctgt gtggcatcga tgacgacgac tacgcattcc gcgaagcgga
     4021 ttgcggtcag cgcgctcgcg atcgccagct gttcgagcgc gtggccggcc gcccgggccc
     4081 gcttgcgcag gcccgcggta tcgtggagaa gaaccgttcg gccctctgct gcgaatgcgg
     4141 cggccaccga gtcgcgtgta atgcccgctt cagggccagt cagtgcg
//
LOCUS       DASZMC010000651         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1906002,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000651 DASZMC010000000
VERSION     DASZMC010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906002"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VG274_13215"
     CDS             <1..333
                     /locus_tag="VG274_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HWE07666.1"
                     /translation="WRYVMAKRKYKSDAFEAIHSSVSGMYRAGTVGKATMRDFDEACF
                     SMPAEIKPAQIKRLRERNHVSQPVFARYLNTSESTVEKWETGAKRPSGMALKLLAIID
                     KHGLQVLT"
     gene            460..762
                     /locus_tag="VG274_13220"
     CDS             460..762
                     /locus_tag="VG274_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07667.1"
                     /translation="MRAYVVISALFLTAAFPAAAQVSPSSCERPPASQTPPTQRLLLM
                     RNWVKQACAADKAAFCAAVPPGCGRPMRCLRAHGDQLASDCRTALEQLHAAAVAKY"
     gene            complement(776..2203)
                     /locus_tag="VG274_13225"
     CDS             complement(776..2203)
                     /locus_tag="VG274_13225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWE07668.1"
                     /translation="MWISQTAGDVCAARAAGAVRHERPALVLAASILASSLAFVDGSV
                     VNVGLPAIGRDLHGAASDLQWVVNLYLLPLSSLLLLGGAFGDRFGRRNILVLGILVFA
                     AGSALCAASPALAWLLAARTLQGIGAAFVLPNSLAVLGSAWSGAARGRAIGIWSASSA
                     ITAAIGPVLGGWLIDTVGWRAIFLVNLPLAAAAIALALGYVRDPPRSDAKQPLDAWGA
                     GLATASLTALTWSLTAGDSSTEWAGRVLLVASMGVAFAAIFLWTEKQRRENAMMPLAL
                     FASADFVGLTGLTFLIYGALGALLVLVPFVLIRGADYSATLAGAALLPFPLVLAAASP
                     ALGALAGRIGSRIPLVAGPLIVAGGFLLLLRLDPAARFWSELVPAILAIALGMACVAA
                     PLTTAVLASVNARHTGLASGLNSAVARSGGMVATALVGGVFAAKGDALFGAFHAAVIA
                     CAIASALAGGFAFALIGARARPRPP"
     gene            complement(2238..2723)
                     /locus_tag="VG274_13230"
     CDS             complement(2238..2723)
                     /locus_tag="VG274_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07669.1"
                     /translation="MDSPAFTFFHVAISLIAILSGLVVMYGFLAARKLEGVTLIFLVT
                     TVATSVTGFFFHRDHLLPSHIVGAISLLLLASAIVAHYSFHARGAWRIVYVACIVASF
                     YLNVFVLIVQSFQKVPFLHALAPTQSSEPAFVAAQGLALVAFITAGAISLMRFRAPAV
                     A"
BASE COUNT          614 a          917 c          944 g          394 t
ORIGIN      
        1 tggagatatg tcatggcgaa gcgaaagtat aagagcgatg cctttgaggc gatccacagc
       61 agcgtatccg ggatgtatcg tgctggcacg gtcggcaagg cgacgatgcg ggatttcgat
      121 gaagcctgct tttcaatgcc ggcagagatc aagcccgccc aaatcaagag acttcgcgaa
      181 cgcaatcatg tcagccagcc ggtgtttgcc cgctatctca acaccagcga aagcacggtg
      241 gagaaatggg aaaccggagc caagcgaccc agtggcatgg cgctcaaact gcttgccatt
      301 attgacaagc atggcttgca ggttctgaca tagcggtcga cgtctccgtt catccatcgg
      361 cggagtctca aactgcacca aaatccttcc ggcccgcgtt gccggttttc cgggcgcgga
      421 tcatcatggg gcggcgagcg gcgacgaaca ggagatatca tgcgtgcata tgtcgtcatt
      481 tcggctttgt tcctgaccgc tgctttcccc gccgcggcgc aggtttcgcc gtcgtcctgc
      541 gagcgccctc ccgcttcgca gaccccgccc acgcagcgac tgttgctgat gcgcaactgg
      601 gtgaagcagg cttgcgccgc cgacaaggcg gcgttctgcg ccgcagttcc gcccggctgc
      661 ggacgaccga tgcgctgcct gagggcgcat ggcgaccagc tggcgtccga ctgcagaacc
      721 gcgctcgaac aattgcacgc cgcggcggtg gcgaaatact gagcgctttt gacacttaag
      781 gcggtcgcgg ccgcgcgcgg gcgccgatca atgcgaaggc gaaaccgccc gccagcgccg
      841 aggcaatcgc gcaggcgatg acggcggcgt gaaacgcgcc gaacaacgcg tcgcctttcg
      901 cggcgaagac gccgccgaca agcgcggtgg cgaccatgcc gccgcttcgc gccaccgcac
      961 tgttcagtcc cgacgccaat ccggtgtggc gcgcattgac ggaggcaagc accgccgtgg
     1021 tgagcggcgc cgcgacgcac gccattccca gagcgatggc gagaatcgcg gggacgagtt
     1081 cgctccagaa gcgcgcggcg ggatcgagac gcagcagaag aagaaaacct cccgcgacga
     1141 tgagcggacc ggcgacgagc ggaatgcgcg agccgatacg gcctgccaag gcgccgagtg
     1201 cgggcgacgc cgcggcgagc acgagcggga acggcaggag cgccgcgccg gccagcgttg
     1261 ccgaataatc cgcaccgcgg atgagaacga agggcacgag caccagcagc gcgccaagcg
     1321 cgccgtagat gaggaacgtc aagcccgtca gtccgacgaa atcggcggaa gcgaacaggg
     1381 caagcggcat catcgcattt tcgcggcgct gtttttcggt ccagagaaag attgcagcga
     1441 aggcaacacc catcgaagcg accagcaaca cgcggccggc ccattccgtc gaactgtctc
     1501 cggcggtcag gctccaggtc aacgcggtga gcgacgccgt cgcaagcccg gcgccccacg
     1561 catccagcgg ctgctttgcg tcactgcgcg gcggatcgcg gacatagccg agcgccagcg
     1621 caatggctgc agcggcaagc ggcaggttga cgagaaagat cgcgcgccag cccaccgtat
     1681 cgatcagcca gccgccgagg accggcccga ttgcggccgt gatcgcgctc gacgccgacc
     1741 agatgccgat cgcgcggccc cgcgcggcgc cggaccaggc gctgcccagc accgccaggc
     1801 tgttcggcag cacgaaggcc gcgccaattc cctgcagggt gcgcgcggcc agaagccagg
     1861 caagcgcggg agacgcggcg cagagagcgg aaccggcggc aaaaaccaat atgcccaaaa
     1921 cgaggatgtt gcgccgcccg aagcggtcgc cgaacgcgcc gcccagaagc aggagcgagc
     1981 tcagcggcag gaggtagaga ttgaccaccc actgcagatc cgaagcggcg ccgtggagat
     2041 cgcggccgat cgccggcaac ccgacattca ccaccgatcc atcgacgaag gcgaggctgg
     2101 aggcgagaat gctggcggcc agaacaagcg ccggtcgttc gtggcgcacc gcgccggcag
     2161 cgcgcgcggc gcagacatcc cccgccgtct ggctgatcca caccaaacag aagcgctccg
     2221 tacctgtcct gtcttaccta tgccacagca ggcgcccgga aacgcatcag cgatatcgcg
     2281 ccggcggtga tgaaggcgac gagcgccagg ccttgcgccg ccacgaaagc tggctcgctc
     2341 gactgcgtgg gagccagtgc gtgcaggaac ggcaccttct ggaaggactg aacgatcagc
     2401 acgaagacgt tgagatagaa cgaggcgaca atacaagcca catagacgat gcgccacgcg
     2461 ccgcgtgcat ggaacgaata atgagccacg attgcgctgg ccagaagcag cagcgagatc
     2521 gcccccacga tgtgggacgg caggagatgg tcgcgatgga agaagaagcc cgtgacgctc
     2581 gtcgccaccg tggtgacgag aaagatgagc gtcacgcctt cgagcttcct ggcggcgaga
     2641 aatccataca tgaccacaag gcccgaaaga attgcgatca ggctgattgc aacgtgaaaa
     2701 aacgtgaatg cgggcgaatc catatatccc tccctcgctc ggcgcgcggc accctatacc
     2761 gtggcgagcg ttggcgcagg ttttttcatg aacccgagcg aggttgctca ctcggtttac
     2821 ctcccccatc gtgggggagg cggtttgcga agcgaagcgg agcaaaccg
//
LOCUS       DASZMC010000652         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1906347,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000652 DASZMC010000000
VERSION     DASZMC010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906347"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2223
                     /locus_tag="VG274_13235"
     CDS             <1..>2223
                     /locus_tag="VG274_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWE07670.1"
                     /translation="EGYLALDSEWRITYLNTAAERLIGMRRGALLAPDYRDLLARVPL
                     PAAFEHQDSGSDRCFQIKAYPAPGGAISLFIEDITTRNQARRQAESLNDLALAISGEL
                     ELEKIAHAVTGAATALTQAEFGAFCDFPKAHLEPRIVYALPGDSREDLQNLAVQFAPA
                     FREKRIVRGNEIRGLPVLSYLAVPVVSRNGEVLGGLLLAHSGANVFTEASERLATTIA
                     GFAAGAIDNSRLFARAEQEIANRRRAEELLRESENRFRQMIDALPAAIYTTDAHGYLT
                     HFNPAAVELSGRVPELGSDRWCVTLRLYNADGTPLPHEQCPMAAALKEQSVAPGQECI
                     AERPDGSRFWFTPYPTPLRDKERRIVGAINMLVDITARKQAEEALRRSQERFRGVFES
                     SAIGVAVLTLEHRIVEANSAFCTISGYSETELDGLDSTCLIHPGDVARARDLSARLIS
                     GAIPSFTVEQRCLTKDSRAIWVCNSFSLMRDAAGRPEYLIALCEDITLRKRIEADLRE
                     SEERSRAIVETTPECVKVVARDGTLLQMNGAGLAMVSAARAENVVGQSVFSLIAPEFR
                     DRYRAFHESVCGGRRGSMEFDIVGLSGERRRMESHAAPLRKPDGTTVNLSISRDVTDR
                     KRKDRVTLLLGAIVNSSHDAIVSKDLNGVITSWNQSAERLFGYTAEEAIGQPVTMLIP
                     LDRQEEEPLILERLRGGETIDHFETIRRRKDGTLFHVSLTISPVRDEAGTVIGASKIA
                     R"
BASE COUNT          445 a          761 c          628 g          389 t
ORIGIN      
        1 cgaaggctac cttgcactcg attcagaatg gcgcattacc tacctgaaca ccgcagccga
       61 gcggttgatc ggcatgcgtc gcggagcact gctggccccc gactaccgcg acctcctcgc
      121 ccgcgtgccc cttccagccg cctttgaaca tcaggactcc ggctcagacc gctgcttcca
      181 gatcaaggcc tacccggcgc ccggcggcgc gatctctctc tttattgaag acatcacaac
      241 ccgcaaccag gcgcgccggc aggccgaaag cttaaacgac ctcgccctcg ccatctccgg
      301 cgaactcgaa ctcgagaaga tcgcgcacgc cgtgaccggc gccgcaaccg cactcacgca
      361 agcggaattc ggcgccttct gcgattttcc gaaggcacac ctcgaacccc gcatcgtata
      421 cgctcttccc ggcgactcgc gtgaagacct ccaaaaccta gccgtccaat tcgcgccggc
      481 cttccgcgaa aaacgcatcg tccgcggtaa cgagatccgc ggtttgcccg ttctcagcta
      541 tctcgcggtc cccgtcgtaa gccgaaacgg cgaagtgctc ggcggattgt tgctcgccca
      601 ctccggcgcg aacgttttca ccgaagcatc tgagcgcctc gccaccacca tcgccggttt
      661 cgccgccggc gccatcgata actctcgtct attcgcgcgc gctgagcagg aaatcgccaa
      721 tcgtcgccgc gcggaggaac ttcttcgcga aagcgagaac cgcttccgcc agatgattga
      781 cgcgctgccc gccgccatct acacgaccga cgcgcacgga tacctcacgc acttcaatcc
      841 cgccgccgtg gaactctcgg gccgcgtccc cgagttgggc tcggatcgct ggtgcgtaac
      901 tttgaggctc tacaatgccg acggcacgcc cctcccgcac gaacaatgcc ccatggccgc
      961 cgctctcaag gagcagagtg tagcccccgg tcaggaatgc attgcggaac ggcctgacgg
     1021 ctcccgcttc tggtttactc cgtatcccac gccgcttcgc gacaaagaac ggcgcattgt
     1081 cggagcaatc aacatgctgg tcgacatcac ggcccgcaaa caggcggaag aagcgctccg
     1141 ccgcagtcaa gagcgttttc gcggcgtctt cgaatcgtcg gccatcggcg tagccgtcct
     1201 gacgctcgaa catcgcatcg tcgaggcgaa cagcgccttc tgcaccatct ccggatacag
     1261 cgaaaccgaa ctcgacggtc tcgattcaac ctgcctcatt caccccggcg atgtagcgcg
     1321 tgcccgcgat ctgagcgccc ggctcatatc cggcgctatc ccgtcattca ctgtcgaaca
     1381 gcgttgtttg acgaaggaca gccgcgccat ctgggtttgc aacagcttct cgcttatgcg
     1441 agacgcggcg gggcggcccg aatatctgat cgcgctctgt gaggacatca ccttgcgcaa
     1501 acgcatcgaa gcggacttgc gcgaaagcga ggagcggtca cgagccatcg tcgagaccac
     1561 tcccgaatgc gtcaaagttg tggcgcggga cggaacgctg ctgcagatga atggcgccgg
     1621 gctggcgatg gtaagcgccg cgcgcgccga gaatgttgtc ggtcagagtg ttttttctct
     1681 gatcgctccc gagttccggg accgctaccg ggcctttcac gaaagcgtct gcggcggccg
     1741 aagaggctca atggagttcg acatcgtcgg cctgagcggc gagcgcaggc gcatggaaag
     1801 ccacgccgca ccgctccgaa aaccggatgg aaccacagtg aacctctcga tcagccgtga
     1861 cgtgacagat cgcaagcgta aggatcgcgt caccctcctg ctcggggcca tcgtgaattc
     1921 atcgcatgac gcgatcgtca gcaaagacct gaacggcgtg atcacgagtt ggaaccagag
     1981 cgccgagcgc ctgttcggtt acaccgccga agaggctata gggcaaccgg tgaccatgct
     2041 catcccgctg gatcggcagg aggaagaacc cctcattctg gaacgcctca ggggcggtga
     2101 aacgatcgat catttcgaga cgatccgccg gcgcaaagac ggcacgctgt tccatgtctc
     2161 gctcacaatt tcgcccgtcc gcgacgaagc gggaacggtc atcggcgctt ccaagatcgc
     2221 ccg
//
LOCUS       DASZMC010000653        10843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1906793,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000653 DASZMC010000000
VERSION     DASZMC010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906793"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VG274_13240"
     CDS             <1..1176
                     /locus_tag="VG274_13240"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWE07671.1"
                     /translation="PAAAANTGTDLLPGALPASSAGRLPSTSEQYRALRETIAKSRPA
                     VEVAKSRSDLLNSQAADLKRRLIATAARVQDLEEENLRLGADIARLSKDDEALTASFR
                     RQRAQVATLLAVLERVQHDMPPVMAIRADDALAGAHVSMLLGAGLPRLYSAAAELSHR
                     LDLLRRTRADLVRRREGAARITTALAGARDDLDQLLATKSRQAGEAGAQYAELASKLE
                     VVASQAVSLESLLRKVALLRSAPSTRNLMIVTSPAAAANQKLQRGAFLRPVTGNMVEG
                     DGEAAGAPRAPGVSFLAPASAQVVAPADSKVLFAGPYHKTGQVLILQMTGGYALVLAG
                     LDRVEVRTGDQLLAGEPVGRMPRNGAENRLYFELRENGKGVNPAPWLEVELRKATRS"
     gene            1173..2474
                     /locus_tag="VG274_13245"
     CDS             1173..2474
                     /locus_tag="VG274_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWE07672.1"
                     /translation="MKRFAFVGVGMVAGFSAALMLFPAAHGANDSAYKQLDLFSDAFE
                     RVRADYVHPVEDGQLVTSAIEGMVSGLDPHSSYMDAKAFADMKIQTKGQFGGLGIEVT
                     MEDGLVKVISPIDDTPAARAGIKPGDYIAAIDSTPIQGMSLNDAIEKMRGPEGSKISL
                     TILRSGAKKPFDVALTRAIVQVDSVKFHRDGDIGYIRLSAFNEATAPGLERAVRDLKR
                     QIGPGLKGYVLDLRNNPGGLLDQAIQVSDDFLTSGEIVSTRGRHSDDTARYDAKPGDI
                     GDGKPIIVLVNAGTASASEIVAGALQDHKRATIEGMTSFGKGSVQTIIPLGEGGGALR
                     LTTARYYTPSGHSIQATGIVPDVAVAEGDEDETVARLEHLSEANLPGHLNGEGGAKKT
                     APQIIHPPAGKKIEDFQLSYALDILHGKLAMSASNKSAAAN"
     gene            2534..3844
                     /locus_tag="VG274_13250"
     CDS             2534..3844
                     /locus_tag="VG274_13250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWE07673.1"
                     /translation="MRRMALTALGAAAGLAAGLIVLPLARSATDRDAQSAYGALDRFG
                     AAFATVRADYVETPDDRKMIESALNGMISDLDPHSSYFDPKTFSDMQVKTSGTYGGVG
                     LVISAEEGIIKVVSPIDDTPASHAGIKAGDTILAIDGRGLQGQTLDKVQDMLRGAAGT
                     KVGLTLLRAGNQKSFEVGLTRAAIQVEAVKFHREGDVGYIRIPAFNEETSDGVRAAVK
                     NLKGQIGPRLKGFIIDLRDDGGGVLDQAIAVSDDFLDAGEIVSTRGRKPEDTQRYDAK
                     PGDIADGKPLTVLIDAGTASASEIVAGALQDHKRATIVGMTSFGKGSVQTIIPLRGGE
                     DGALHLTTARYYTPSGRSIQASGIVPDIAVAQGDEAAEVPKIERPSEADLPHHLTAEQ
                     PPSQPKLNVVRPPAGKKTDDFQLAYAVKLMDGKMPEAGKRTSAR"
     gene            3917..4417
                     /locus_tag="VG274_13255"
     CDS             3917..4417
                     /locus_tag="VG274_13255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HWE07674.1"
                     /translation="MSLRARPLSHDDLPYRPSVGIMLLNRSGLVFVGKRIDQRAEGWQ
                     LPQGGIDEGESAVDAGFREMEEEIGTRNAVLLRELDAWLSYDLPPHLLGVALRGRYRG
                     QRQRWLALRYLGEDSDIDINTAEPEFARWKWIDLETLPRIVVPFKRNIYATVIEAFRD
                     LARPAQ"
     gene            4428..5225
                     /locus_tag="VG274_13260"
     CDS             4428..5225
                     /locus_tag="VG274_13260"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWE07675.1"
                     /translation="MTRSPVVMIHGAFCGGWAFERWRAPYESRGFEVFTPTLRHHQPS
                     STSPDMLGRTSLRDYASDLANLLDTLGTAPVIVGHSLGGLLAQMLAALKPVRALVLLA
                     PSAPWGMLPSTSFEFYSAQALYLDGPFWSRPLTPRRWIAEANMLDLVPESQRAAILAR
                     LVPESGRATFEAMHWMLDVGRASHVDARAVVCPILCLAGAQDRVTPPSTVRRIARRYH
                     GRARFEEIPGHSHWLIGEPGWEKIAAGTLTWLDQVLDHDEQRTPSRT"
     gene            complement(5347..6972)
                     /locus_tag="VG274_13265"
     CDS             complement(5347..6972)
                     /locus_tag="VG274_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07676.1"
                     /translation="MKFCGYLASGVSALALAAMTSFSTGAAARQIHYPFKMGVHFKVA
                     RQKGPLEAPYVAPSAAKSGTWTDVGNLPFADGPWDPLLLTDGTVIIEDFCTSPTQWYR
                     LTPDKKGKYESGKWSKIATMPSGYAPLFFASQTLTDGRLIVNGGEYNATANGNCGSGA
                     WTTKGALYDPVKDKWASVSPPTGWGNIGDAQSVILPDGSYMLADCCDEAQAIATISGT
                     TVTWNTTGSGKADENDEEGWTLLPSGNVLVVDAWLGVAHTQSNSEIYDTSKGTWSAGP
                     LTASQLVDPGSHELGPAVLRPDGNIVYFGGNQAGPINVFNEAANTWVAAPSFPVINGA
                     QYDVADGPAALLPDGNVIVDASPGVFNAPSHFFEFSINAKGVMKLTQVNDPKQAPGTS
                     SFEGSFLDLPTGQVVWTDSQTTPNEVATYTPRGSPKAAWLPKISSVSATLSVGRKKNA
                     ISGKNFNGFSLGASYGDDAQEATNFPIVRITNTGTGDVCYGRSYNFSTMGVFNTGKMH
                     ADFDVPASCETGDSTLQVIVNGLASAGVAVTLS"
     gene            complement(7128..7877)
                     /locus_tag="VG274_13270"
     CDS             complement(7128..7877)
                     /locus_tag="VG274_13270"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HWE07677.1"
                     /translation="MPLLWIMLVASPFIGSFLGVLALRLPEGRSFVASRSVCDRCGHV
                     LAARDLVPIASFLALRGRCRYCGARIGGFVLFIELAAVLVAGWAGMQISGWVLVATCF
                     LGWTLLLLAIIDWRVQLLPDVLTLPLLAAGLVVTYAVSSETWPDHLIGAVAGFVTLAA
                     IALVYRRLRGREGLGLGDAKLLAALGAWVAWQGLPTVLLWGSMLGLVFALVRSMTGRG
                     LQWSDRIPFGVFLAAGGWLVWLYGPLMPALR"
     gene            complement(7877..8116)
                     /locus_tag="VG274_13275"
     CDS             complement(7877..8116)
                     /locus_tag="VG274_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWE07678.1"
                     /translation="MPPLDLRGLKCPLPALLARRALMRAEAGTPLGVLADDPLAAIDI
                     PHMCSQEGYEVLDVEKHAAHMRFVVRRPLTGTRRR"
     gene            8187..9977
                     /locus_tag="VG274_13280"
     CDS             8187..9977
                     /locus_tag="VG274_13280"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HWE07679.1"
                     /translation="MDVFSAVSRESTYIASLARTFWRLRGIKPQSPVTIVDIVEGWAR
                     RTPANPAILCGDDTITYDALNAGADRYACWAHVQGIGRGDCVALLMENRPEYLFAWLG
                     LFKVGAIAALINTNLRGGALAHSIAISGARHIVIGAELAQEYLDARSALAPPPVGWIT
                     GGMQPGLENLDAALAAAGSAPANPKWRAGVICADKAFYIFTSGTTGLPKAANVSHLRT
                     LFMMYGFAGALGARASDRMYDVLPLYHSAGGICAIGVALTCGGSVVVRRKFSVHEFWD
                     DCARYRPTFFQYIGELCRYLLNAPVSPRERNHHLRAIIGNGLRPEIWKPFQTRFAIPK
                     IIEFYGATEGNVSMLNYDGKIGAVGRIPDYMRGYFAIRIIRFDIETEIPVRNPEGFCI
                     ECAVGETGEAIGRIVDEPGKSFEGYTHAADTEKKILRHVFAADDSWFRTGDLMRRDAL
                     GYFYFIDRVGDTFRWKGENVATSEVAEALSVVDGIVEANVYGVRVPANDGRAGMAALV
                     TAAGFDPSRLADQLQGSLPAYARPVFLRLMPEMEITGTFKQRKIDLMKDGFDPRRIAD
                     PLYALDPQTLVYTPLDAATYDDILAGRLKL"
     gene            complement(10149..>10843)
                     /locus_tag="VG274_13285"
     CDS             complement(10149..>10843)
                     /locus_tag="VG274_13285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWE07680.1"
                     /translation="ASRNIEFGLRGKFATDHLPGMFSWSASAYRTDAGRDIQLLATQI
                     NGFGFFSNTGTTRHQGADAHLGYHDGRWRIDAGYSWLDATFLGAETISSNSPAANAQG
                     LISVRPGDRLPLNPEHRFTLSADFAVTSAWSVGADVRAQSGQYLAGDESNQEQQLPGY
                     GRLDVRTSWRVDPRLELFGEIENLLDQRTYTYGAFAQLAGLPQNFDLTDPRTYSPAPG
                     RLFFAGVRAQLD"
BASE COUNT         2048 a         3459 c         3426 g         1910 t
ORIGIN      
        1 ccggctgcag cggcaaatac gggaacggat ttgctcccgg gcgcactccc cgcctcatcg
       61 gcggggcgac ttccaagcac atcggaacaa tatcgggcgc tgcgggagac gatcgcgaag
      121 tcccggccgg cggtggaggt ggccaagagc cgcagcgatc ttctcaactc ccaggccgcc
      181 gatctcaagc gccgtctgat cgcaaccgcc gcgcgagtgc aggatctgga agaggaaaat
      241 ctccggctcg gcgcggacat cgcaagattg tcgaaagacg acgaggcgct cactgctagc
      301 ttccggcgcc agcgagcgca ggtggccacc ctcctcgcgg tccttgagcg cgtgcagcac
      361 gacatgcctc cggtgatggc aattcgcgcc gacgatgcgc tggcgggcgc ccatgtttcc
      421 atgctgctgg gcgcgggcct gccacgcctc tatagcgccg cggcggaact gtcgcacaga
      481 ctcgatctgc tgcggcgcac gcgcgccgat ctcgtccgcc gccgcgaagg cgcggcgcgg
      541 atcaccaccg cgcttgcggg cgcgcgcgac gatctcgatc aacttctcgc gacgaagtcc
      601 cggcaggccg gcgaggccgg cgcgcaatac gccgagctcg cttcgaagct ggaggtggtc
      661 gccagccagg ctgtcagcct cgaatctctg ctcaggaagg tggcgctatt gcggtccgcg
      721 ccttccacac ggaacctgat gatcgttact tcaccggcgg ctgcggccaa tcaaaagcta
      781 cagcggggcg cttttctcag gccagtcacg ggcaacatgg tcgagggcga cggagaagct
      841 gccggcgcgc cccgcgcgcc tggcgtgagc tttcttgccc cggcgtcggc ccaggtcgtc
      901 gcgcctgccg attcgaaagt gcttttcgcc ggcccttacc ataaaaccgg tcaagtcttg
      961 atcttgcaga tgacgggcgg atacgctctt gtgcttgcgg gcctcgatcg tgtcgaagta
     1021 cggacaggcg atcagttgct ggcaggcgaa ccggtcggca gaatgccgcg aaatggtgcg
     1081 gaaaaccggc tctattttga attgcgggaa aacggaaagg gcgtcaatcc ggcgccatgg
     1141 ctggaagtcg aactgagaaa ggcgacaaga tcatgaagcg tttcgcattt gtgggcgtcg
     1201 ggatggtggc aggattttct gctgcgctga tgcttttccc ggccgcgcat ggcgcaaacg
     1261 attcggccta caagcagctc gatctgttca gcgacgcgtt cgagcgcgtg cgcgcggact
     1321 acgtccatcc ggtggaagac ggccagctcg tcacctcggc catcgaaggc atggtgtccg
     1381 ggctcgatcc gcattcgagc tatatggatg caaaagcctt cgccgacatg aagattcaga
     1441 ccaaaggcca gttcggcggc ctcggaatcg aggtcacgat ggaggacgga ctcgtcaagg
     1501 tgatctcccc gatcgacgac acgccggccg cacgcgccgg catcaagccg ggtgactata
     1561 tcgccgcgat cgacagcacg cccatccagg gcatgtcgct taacgacgcc atcgagaaga
     1621 tgcgcggacc ggaaggctcg aagatctcac tgaccatact gcgcagcggc gccaagaaac
     1681 ccttcgacgt ggcgctgacg cgcgcgatcg tgcaggtgga cagcgtgaaa ttccatcgcg
     1741 acggcgatat cggctatatc cgcctgagcg ccttcaacga agcgacggcg ccggggctgg
     1801 agcgtgcggt acgcgatctg aagcggcaga tcggacccgg cctcaaaggc tatgtgctcg
     1861 atctcaggaa caatcccggc ggtctgctcg atcaggcgat ccaggtctcg gacgacttcc
     1921 tcacctccgg cgaaatcgtt tcgacaaggg gtcgccactc cgacgacacg gcgcgctatg
     1981 acgcaaaacc cggcgacatc ggcgacggga aaccgatcat cgtcctcgtc aacgcgggca
     2041 ccgcctcggc atcggagatc gtcgctggcg cgcttcagga tcacaagcgc gcgacgatcg
     2101 aaggcatgac gtcgttcggc aagggctccg tgcagacgat catcccgctt ggcgagggag
     2161 gtggcgccct gcgcctcacg acggcgcgtt attatacgcc atcgggtcat tcaattcagg
     2221 cgaccggaat cgttcccgac gtggccgtcg cggaaggcga cgaggacgag accgtcgcca
     2281 ggctcgagca tctgagtgaa gccaatctgc cgggacatct caacggggaa ggcggcgcca
     2341 agaaaaccgc gcctcagatc attcatccgc ctgccgggaa gaagattgaa gacttccagc
     2401 tctcttatgc gttggacatc ctgcacggca aactggcgat gagcgcttcc aacaagagcg
     2461 ccgcggcaaa ttgagcgccg cgcgcaggat gacagttcgc gggcaaggac tgaccggcaa
     2521 aggataagtc tgcatgcggc gaatggctct cacggcactg ggtgcggcgg cgggactcgc
     2581 cgccggcctc atcgtgttgc ctctcgccag aagcgcaacg gaccgcgacg cccagtcggc
     2641 atacggggcg ctcgaccgtt tcggcgcggc gttcgcaacc gtgcgagcgg attatgtcga
     2701 gacacccgac gatcgaaaga tgatcgagag cgcgttgaac ggcatgattt ccgatctcga
     2761 cccgcattcg agctacttcg acccgaaaac attttccgac atgcaggtca agacttcggg
     2821 gacctatggc ggcgtcggtc tggtgatctc cgccgaggag ggcatcatca aggttgtttc
     2881 gcccatcgac gacacgcctg cctcgcacgc gggaatcaag gcaggcgaca cgatcctcgc
     2941 gatcgacggg cgcgggcttc aaggccagac cctcgacaag gtgcaggaca tgctgagagg
     3001 cgcggcgggc accaaagtcg gcctcacgct gcttcgcgca ggaaatcaga agtccttcga
     3061 ggtcgggctg acgcgcgccg ccatccaggt cgaggcggtg aaatttcacc gcgaagggga
     3121 cgtgggttac atccgcatcc cggccttcaa cgaggaaacg agcgacgggg tccgggcggc
     3181 ggtcaagaac ctcaagggtc agatcggccc gcgtctcaaa ggcttcatca tcgatttgcg
     3241 cgacgatggc ggcggcgttc tcgaccaggc gattgccgtt tccgatgatt ttctggatgc
     3301 gggcgagatc gtttcgacgc gcgggcgcaa gccggaagac acgcagcgtt acgacgccaa
     3361 gccgggcgat attgccgacg gcaagccgct caccgtgctg atcgacgccg gcacggcttc
     3421 ggcgtccgaa atcgtcgccg gtgcactgca ggatcacaag cgtgcaacga tcgtcggcat
     3481 gacgtcattc ggcaagggat cggtgcaaac catcattccg ctgcgcggcg gcgaagacgg
     3541 cgcgttgcac ctcacgacgg cgcgctatta cacgccttcg gggcggtcga tacaggcgtc
     3601 cggcatcgtc cccgacattg cggtcgcgca aggcgacgaa gcggcggagg tcccgaagat
     3661 cgaacggccg agcgaagccg atctgcctca ccatctgacc gcagaacaac ctccgtccca
     3721 gccgaaattg aacgtggtgc gccctcccgc cggaaagaag accgacgact tccagctcgc
     3781 ctatgcggtg aaactgatgg acggaaaaat gcccgaagca ggaaagcgca ccagcgcgcg
     3841 ctgaatcggt ctgggctttg ttcccggccc tagttcgtcc atagtggcgg catccgcaga
     3901 ttggcagact tcccgcatgt ctttgcgcgc ccgccccctc agtcacgacg atcttcccta
     3961 tcgccccagt gtcggcatca tgctgctcaa tcgcagcggg ctggtgttcg tcggcaagcg
     4021 catcgatcag agggcggagg gctggcaatt gccgcagggc gggatcgacg agggcgagag
     4081 cgccgtcgat gccggtttcc gggaaatgga ggaggagatc ggaacgcgca atgccgttct
     4141 gctgcgcgag ctcgacgcat ggctttctta cgatctgccc ccgcaccttc tcggcgtcgc
     4201 tttgcggggg cgctatcgcg gacaacgcca gagatggctg gccttgcggt atctggggga
     4261 ggattccgac atcgacatca acaccgcgga gcccgaattt gcgcgctgga aatggatcga
     4321 tctcgaaact ctgccgcgca tcgtcgtccc cttcaagcgc aatatctacg cgacggtcat
     4381 cgaagcgttc cgcgatctcg ccaggcctgc gcaatgagcc gttcgtcatg acacgttctc
     4441 ccgtcgtcat gattcacggc gctttctgtg gcggctgggc gttcgagagg tggcgcgcgc
     4501 cttacgagtc tcgcggcttc gaggtgttta cgcccaccct gcggcatcat cagccctcga
     4561 gcaccagccc ggatatgctc ggcaggacaa gcctgcgcga ctatgcatcg gatctcgcaa
     4621 acctcctcga cacgctcggg accgcacccg ttatcgtcgg acactccctt ggcgggctgc
     4681 tcgcccagat gctggcggcg ctcaagccgg tccgggcact cgtgcttctg gcgccgtcag
     4741 cgccctgggg catgctcccg tcgacttcgt tcgagtttta ttccgcacag gcgctttatc
     4801 tcgatggacc cttctggagc agaccgctca cgccccggcg ctggattgcc gaggcgaaca
     4861 tgctggatct cgttccggaa tcgcagcgcg cagccattct cgcgcgcctc gtgcccgaat
     4921 cgggccgtgc caccttcgag gcgatgcact ggatgctgga tgtcggacgg gcaagccatg
     4981 tcgacgcgcg cgcagtcgtc tgtccgatcc tgtgcctggc gggagcgcag gaccgggtga
     5041 cgccaccctc tacggtacgt cgcatcgcgc gccgctacca cggacgtgcg cgtttcgagg
     5101 agattccagg tcacagtcat tggctcatcg gcgagcccgg ctgggagaag atcgccgcag
     5161 ggaccctcac ctggctggac caggtcctcg accacgacga gcagcgcacg ccgagcagaa
     5221 cgtagcgccg atcgagtttc ttggaggcag cgattcgcgc gccgcccaat acaaataatc
     5281 atgggcgggt ttccccgccc atgattcaat cgacgcactc tcgcgacggc cttgtactgc
     5341 ttgtcgtcag ctcaatgtca ccgctactcc agccgacgca agtccgttca cgatgacctg
     5401 aagtgtacta tcgccggtct cgcacgaagc gggcacatcg aaatcggcat gcatcttgcc
     5461 tgtattgaat acgcccatcg tggagaaatt gtagctgcgg ccgtagcaaa cgtcgcccgt
     5521 gccggtgttg gtaatgcgca cgatcggaaa gttcgtcgcc tcctgcgcat catcgccata
     5581 ggaagcgccc agcgagaagc cgttgaagtt cttgcccgag atggcgttct tcttgcgtcc
     5641 caccgagagc gtcgccgaca cgctggagat cttcggcagc catgccgctt tcggtgaacc
     5701 cctgggcgta taggtcgcga cttcgttggg tgtcgtctga ctgtctgtcc acacaacctg
     5761 gcctgtcggc agatcgagga acgatccctc gaacgaggag gtgcctggcg cctgtttggg
     5821 atcgttcacc tgcgtcagct tcattacgcc cttcgcgttg atgctgaact cgaagaagtg
     5881 cgacggtgca ttgaagacgc ccgggcttgc gtccacgatg acattgccgt cgggcaacag
     5941 cgcggccgga ccgtcggcaa cgtcatattg cgccccgttg atgacgggga agctgggagc
     6001 cgccacccat gtgttggccg cctcgttgaa gacgttgatc ggacccgcct ggttgccgcc
     6061 gaaatagacg atatttccgt cgggccgaag cactgccggt ccgagctcgt gggaacccgg
     6121 gtcgacaagc tggctcgctg tgagtggccc ggcgctccac gtgcccttcg acgtgtcata
     6181 gatttccgag ttcgactggg tgtgggcgac gccaagccag gcgtcgacca ccagcacgtt
     6241 tccactgggc agcagggtcc agccttcttc gtcgttttcg tcggccttgc cgctgcccgt
     6301 ggtgttccac gtcaccgtcg tgccgctgat cgttgcgatg gcctgcgcct catcgcagca
     6361 atcggcgagc atgtagctgc cgtcgggcag gatcacgctc tgcgcgtcgc cgatattgcc
     6421 ccagccggtt ggcggcgaga ccgacgccca cttgtctttt accggatcgt aaagcgcgcc
     6481 cttggtggtc caggcgccgc tgccgcaatt tccgttcgcc gtggcattgt attcgccgcc
     6541 atttacgatc aggcggccgt cggtgagggt ttgtgaagcg aagaagagtg gcgcataacc
     6601 gctcggcatc gtcgcgatct tgctccattt gccgctttcg tatttgccct tcttgtccgg
     6661 tgtgagcctg taccattgcg tgggcgacgt gcagaaatct tcgatgatca ccgtgccgtc
     6721 ggtgagcagc aacgggtccc agggcccgtc cgcgaacggc aaattgccca cgtccgtcca
     6781 tgttccggat ttcgccgccg aaggagcgac atagggcgct tccagcggac ccttctggcg
     6841 cgccaccttg aagtggacgc ccatcttgaa cggataatgt atctgtcttg cggccgcgcc
     6901 agtggaaaac gaggtcatag ccgcgagcgc gagcgccgaa acacctgatg ccaggtagcc
     6961 gcagaatttc attggttttc cccctgaaga gccgtccgcc attcggacaa tttcgcgtcc
     7021 cggtgggcgc attttgcgaa aggctacttc gatcgacacg catcggcaag ctgccatgcg
     7081 cgcaaggccg ggtgcccatg gccctttgga aaccgggcaa cgtcttgtca ccgcagcgcc
     7141 ggcatgagtg gcccatagag ccagaccagc cagcctccgg cggcgagaaa cacgccaaag
     7201 ggaatgcggt cggaccattg caatccccgg cccgtcatcg accgaaccag ggcaaagaca
     7261 aggccgagca tcgatcccca gagaagcacc gtcggcaagc cctgccatgc gacccaggcg
     7321 ccaagcgccg ccagcagttt ggcgtcgccc agtccaagac cttcacggcc tctcaaacgc
     7381 cggtagacca gtgcgatcgc ggccagcgta acgaaccccg ccacggcgcc gataagatga
     7441 tcgggccaag tctcggagga gacggcataa gtgaccacca gtcccgccgc cagcagaggc
     7501 agagtgagca catcgggcag gagctgcacg cgccagtcga tgatggcgag caggagcagc
     7561 gtccagccca ggaagcaggt ggcaacgaga acccatcccg aaatctgcat ccccgcccat
     7621 ccggcaacga ggacggcggc aagttcgatg aaaaggacaa acccgccgat gcgcgcgccg
     7681 caataccggc accgtccgcg cagggcgaga aagcttgcga tgggaacgag gtcgcgcgcg
     7741 gcaagtacgt gtccgcaacg gtcgcaaacg gaacgcgacg ctacgaagga tcgcccttcc
     7801 ggaagacgca gcgccagaac gccgagaaaa ctgccgatga atggcgatgc cacgagcatg
     7861 atccacaaca gcggcatcag cgcctgcggg ttccggttag cgggcggcgc accacgaacc
     7921 gcatgtgcgc agcgtgcttt tcgacgtcga gcacttcgta gccttcctga ctgcacatgt
     7981 ggggtatgtc gatggcggcg agaggatcgt cggccagaac cccgagcggc gtgccggctt
     8041 ctgcgcgcat cagcgcgcgg cgcgccaaaa gcgcgggcag cggacatttc aaccctctga
     8101 gatcgagtgg aggcatcttt cgtggacgga ggcgtgcttc gcgacctatc ttgccacgaa
     8161 acgcggaaga ggggcgcgat ttgccgatgg acgtattctc cgcggtttcc cgcgagtcca
     8221 cctatatcgc aagccttgcg cggacattct ggcgcctgcg cggcatcaag ccccaatcgc
     8281 ccgtcaccat cgtcgacatc gtcgagggct gggcgcggag aactccggcc aatccggcca
     8341 ttctctgcgg agacgacacc atcacctacg atgcgctcaa cgccggggcg gaccgctacg
     8401 catgttgggc acacgttcag ggaatcggac gcggagactg cgtcgcgctc ctgatggaga
     8461 accgcccgga atatctgttc gcctggctcg ggctcttcaa ggttggagcg atcgccgcgc
     8521 tgatcaacac caatctgcgc ggcggcgccc tcgcccattc gattgcgatc agcggcgcgc
     8581 ggcacatcgt gatcggagcg gaactggcgc aggaatatct tgacgcccgc tccgcactgg
     8641 cgccgccgcc ggtcggatgg atcaccggcg gcatgcagcc cggcctcgag aatctggatg
     8701 cagcgctcgc cgctgccggc tccgcccccg caaatcccaa atggcgggca ggcgtcattt
     8761 gcgccgacaa ggccttctac attttcacct cggggacgac cgggctgccc aaggcggcga
     8821 atgtcagcca tcttcgcacc ctcttcatga tgtacggatt cgccggagcg ctgggcgccc
     8881 gcgcgtcgga ccgcatgtac gacgttctgc ctctctacca ttcggcgggc ggcatctgcg
     8941 caatcggcgt ggcgctcacc tgcggcggct ccgtcgtggt gcgccggaaa ttctcggtgc
     9001 acgaattctg ggacgattgc gcgcgctacc ggcccacctt ctttcagtac atcggcgagc
     9061 tttgccgcta tctcctcaat gctcccgtgt cgccgcgcga acgcaaccac caccttcgcg
     9121 ccattatcgg caacggattg aggcccgaaa tctggaagcc gtttcagacg cggtttgcaa
     9181 taccgaaaat catcgagttc tacggcgcca ccgaaggcaa cgtgtcgatg ctgaattacg
     9241 acggcaaaat tggcgcggtg gggcgcattc cggactacat gcgcggatac ttcgcgatac
     9301 ggatcatccg cttcgacatc gagactgaaa ttcccgtccg caatcccgaa gggttctgca
     9361 tcgagtgcgc cgtcggcgaa acgggcgaag ccatcggccg gatcgtcgac gagccgggca
     9421 agagcttcga aggctacacg catgcggcgg ataccgaaaa gaagattctg cggcacgtgt
     9481 ttgcggcgga tgattcgtgg ttccgcacgg gcgatctgat gaggagggac gcgctcgggt
     9541 atttctattt catcgaccgc gtcggcgaca cgttccgctg gaagggcgag aacgtggcga
     9601 cgagcgaagt cgccgaagcc ctgtcggtgg tcgatggcat cgtcgaggcc aacgtctacg
     9661 gcgtacgcgt gcccgccaat gacggccgcg ccggcatggc ggcccttgtc accgcggcgg
     9721 gtttcgatcc atcccgcctc gcggaccagc tgcaaggcag cctgcccgcc tacgcccgtc
     9781 cggttttcct gcggctgatg ccggagatgg agatcaccgg cacgttcaag cagcgcaaga
     9841 tcgatctgat gaaggatggt ttcgacccgc gccggatcgc cgaccccctc tatgcactcg
     9901 atccccaaac gctcgtctac acaccgctcg acgcggcgac gtacgacgac atcctcgccg
     9961 gccgcctgaa actctgatgc gtgtactcaa atcggagact cgaagagccc ggagaaaaag
    10021 aaaaccttag cgcagagtac gcagaggaag cgcggtgggc gcaggggaat gtcgcgcgcc
    10081 tcccgcgcgc agtcttctcc accgcggccc cagcgcatcc tctgcgtcgt ctgcgctgaa
    10141 ccctttcttc agtcgagctg ggcgcggacg ccggcgaaaa agaggcggcc gggcgcgggg
    10201 ctgtaggtgc gcggatcggt gagatcgaaa ttctgcggca atcccgcgag ctgcgcgaac
    10261 gcgccatagg tgtaggtgcg ctgatcgagc agattctcga tctccccgaa caattcgagc
    10321 cgcggatcga cgcgccagga ggtcctgacg tcgagcctgc catagcccgg cagttgctgt
    10381 tcctggttcg attcgtcgcc ggccagatac tggccgctct gcgcccgcac gtcggcaccg
    10441 acgctccagg cggaggtgac ggcgaagtcg gccgacaagg tgaaacggtg ctccggattg
    10501 agcggcagcc gatcgccggg gcgaacagag ataagtcctt gcgcatttgc ggcgggactg
    10561 ttcgacgaaa tcgtctcggc gcccaggaac gtcgcgtcaa gccaggaata gccggcgtcg
    10621 attctccatc ggccgtcatg gtagcccaga tgcgcgtccg cgccctggtg ccgcgtcgtt
    10681 ccggtgttgc tgaaaaaacc gaagccgttg atctgcgttg cgagcagctg gatgtcgcgc
    10741 ccggcgtcgg ttctgtaggc gcttgcgctc caggaaaaca ttccgggcaa atggtccgtc
    10801 gcgaatttcc cgcgcagccc gaactcgata ttgcgcgacg cga
//
LOCUS       DASZMC010000654         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1907065,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000654 DASZMC010000000
VERSION     DASZMC010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907065"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VG274_13290"
     CDS             <1..1109
                     /locus_tag="VG274_13290"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWE07681.1"
                     /translation="RASHRAARERLADANALYWEVRQVEARFALEKTLAAACAVPECL
                     ETLKDAGLGRLTKPFVPPPEEDLTPKLRRLAAYARLLKRQADVRARQERAATLEIAGP
                     GRLSHGPLPHPDPEETNPAMAGHSQFKNIMHRKGRADKERSKQFSKLAREIFVSAKMG
                     MPDPNHNPRLRAARITARQLSMPKDNIQRAIDKATGANAENIEEVRYEGYGPGGVAVI
                     VETLTDNRNRTGGDVRSTFSKYGGALGEPNSVTFMFDRVGVMVYPKATGSDDAMLEAA
                     IETGFDECVSSDDDHEFLVSLDGYGTAREALEKKLGQPQSAKIEWRPKTMTPINDSNG
                     ETLIKLLDVLDDFDDVQNIFGNYELSNALMEKIG"
     gene            1129..1590
                     /locus_tag="VG274_13295"
     CDS             1129..1590
                     /locus_tag="VG274_13295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWE07682.1"
                     /translation="MPKIDIASVPERKGAGYPRPFDVPLADRIRQRLGNAGGLTDFGV
                     NLMRLPPGNWSSQRHWHSHEDEFVYILEGEVVLIEDDGEMVLRAGDCAAFPKASGNGH
                     HLINRSSAMALYLEVGSRHPDDMTTCSDIDMKSANSDGRFVHKDGTPYPES"
     gene            1669..>2737
                     /locus_tag="VG274_13300"
     CDS             1669..>2737
                     /locus_tag="VG274_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07683.1"
                     /translation="MSEGLEKLLEQARVRKLDRIAAAYAVAGWLFVQVASIAFPAFDV
                     PGWTLRWLIVAVLAGFPLALFIGWTANRARDTESLRPLKRHEAVLLGTLGLVVVLTIG
                     ELAWHLAAAPAAAPAGASGAPAGSVAVLPFDNISGDPKQKYFSEGISDEIIGLLARNP
                     ALRVAARTSSFFFEGKNEDARTIAHNLNVRSLLEGSVRAEGNRVRIEASLVNAADGYQ
                     IWSQSYDRSLSDILAVQSDIANAIARSLAPTLTGSHPKPSVPKLVQIDPEVYRDYLRA
                     QVFFDQRSFEGNTPESTQAVMQALTLFRSVARRAPDYADGLAAYADALRFSRTGSPQE
                     VSDAIGSALKLDPTNPEALQVS"
BASE COUNT          539 a          866 c          870 g          462 t
ORIGIN      
        1 tccgcgcgtc acatcgtgcc gcgcgggaaa ggctggcgga cgcaaatgcg ctttattggg
       61 aggtccgcca ggtcgaagcg cgttttgcgc ttgagaaaac gctcgccgcc gcgtgcgccg
      121 tgccggaatg cctggaaacc ctgaaggacg cggggctcgg ccgactgacg aagcccttcg
      181 tcccgccgcc cgaagaggac ctcacgccca aactccgccg gctcgcggcc tatgcgcggc
      241 tgctgaaacg gcaggccgat gtccgtgcgc ggcaggaacg tgccgccacc cttgaaatcg
      301 cggggccggg gcggctatct cacggcccgc ttccgcatcc agacccagaa gaaacgaacc
      361 ccgccatggc cggtcattcc cagttcaaga acatcatgca ccgcaagggg cgcgccgata
      421 aggagcgttc caagcagttc tccaagctgg cgcgcgaaat tttcgtctcc gccaagatgg
      481 gcatgccgga ccccaatcac aatccgcgcc tgcgcgccgc caggattacg gcacgccagc
      541 tctcgatgcc gaaggacaac atccagcgcg ccatcgacaa ggcgaccggc gcgaacgcgg
      601 agaacatcga ggaggtgcgt tacgagggct atggcccggg cggcgtcgcg gtgatcgtgg
      661 aaacgctgac cgacaaccgc aaccgcaccg gcggcgatgt gcgctcgacg ttctcgaagt
      721 atggcggcgc gctcggcgag ccgaactcgg taacgttcat gttcgaccgc gtgggcgtga
      781 tggtctatcc gaaagccacg ggcagcgacg acgcgatgct cgaggccgcg atcgagacgg
      841 gcttcgacga atgcgtgtcg agtgacgatg accacgaatt cctggtgagc ctcgacggct
      901 atggcaccgc gcgcgaagcg ctggaaaaga aactcggcca gccgcaatcg gccaagatcg
      961 aatggcgtcc gaaaaccatg acgccgatca acgacagcaa cggcgagacg ctgatcaaac
     1021 tcctcgacgt cctcgacgat ttcgacgacg tgcagaacat tttcggcaat tacgaattgt
     1081 cgaatgcgct gatggaaaaa atcggataat gaagaggagc ggatagcgat gccaaagatc
     1141 gacatcgcgt ccgttccgga gcgcaaaggc gcgggctatc ctaggccatt cgacgtgccg
     1201 ctcgccgatc gtatccgcca acgtttgggc aacgccggcg ggctcaccga tttcggcgtg
     1261 aacctgatgc gcctgccgcc cggcaactgg tcgagccagc gccactggca cagccacgaa
     1321 gacgagtttg tctatatcct cgaaggcgag gtcgtgctga tcgaggacga cggcgagatg
     1381 gtcttgcgcg ccggggactg tgcggcgttt ccgaaggcct ccggcaacgg tcatcacctg
     1441 atcaaccggt cgtccgcgat ggcgctctat ctcgaagtcg gatcgcgtca tccggacgac
     1501 atgacgacgt gctccgacat cgatatgaaa agcgccaact ccgacggccg gtttgtgcac
     1561 aaggacggaa cgccgtatcc ggagtcctga catggattgt ccgaaacccg aatgaccgcg
     1621 caacgcgacc acacgctcgc ccgcactgtt ctatgctaga cacgctgtat gagtgaaggg
     1681 ctggaaaaac tgctggagca agcgcgggta cgcaagctcg acaggatcgc ggcggcctat
     1741 gctgtcgccg gctggttgtt cgtccaggtt gcgtccatcg cgtttccggc gtttgacgtc
     1801 cccggctgga cgctgcgctg gctgatcgtc gcggtccttg ccggatttcc acttgcgctt
     1861 ttcatcggct ggacggcgaa ccgcgcgcgc gacacggaat ccttgcgccc gctcaaacgg
     1921 catgaagcgg ttctcctggg tacgttgggg ctggtcgtgg ttctcacgat cggcgaactc
     1981 gcctggcacc tggcggccgc gcccgccgcc gcaccggccg gggcatcggg cgcccccgcg
     2041 gggtctgtcg cggttctccc cttcgacaac atcagcggcg atccgaagca gaaatatttc
     2101 agcgagggaa tatcggacga gatcatcggc ctgctcgcgc gaaatccggc gctacgcgtg
     2161 gcggcgcgca cgtcgtcgtt cttcttcgaa ggcaagaatg aggatgctcg caccatcgcc
     2221 cacaacctga atgtgcgcag cctgctcgag ggtagtgtgc gtgccgaagg caatcgcgtg
     2281 cgcatcgagg cgtcgctggt aaacgcggcg gacggctatc agatatggtc gcaaagctac
     2341 gaccgctcgc tgagcgacat tctcgccgtg cagagcgaca tcgccaatgc cattgcgcgc
     2401 tcgcttgcgc cgacattgac cggatcgcat cccaagccaa gcgtgccgaa gctggtgcag
     2461 atcgacccgg aggtttatcg ggactatctg cgtgcgcagg tcttcttcga ccagcgcagc
     2521 ttcgaaggca acactccgga aagcacgcag gcggtgatgc aggcgctgac gctctttcgc
     2581 agcgttgccc ggcgcgcacc ggattatgcc gacggactgg ccgcttatgc cgatgcgctg
     2641 cgtttcagcc gcacgggctc cccgcaggag gtatcggacg cgatcgggag cgcactgaaa
     2701 ctcgatccta ccaatccaga ggcgttgcag gtctcgg
//
LOCUS       DASZMC010000655         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_5220629,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000655 DASZMC010000000
VERSION     DASZMC010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5220629"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="VG274_13305"
     CDS             <1..1570
                     /locus_tag="VG274_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07684.1"
                     /translation="LAWFHISKTGAYWHNGATRGYSALAFFNPRTDSAAVVLFNHGPT
                     AFDLTDTLGEHIRARLAGEPALSLRVTPVPPIGGALHALRLFAVYWIVMFAAGAFIYC
                     CVLAVQGLAAQLLPRPLFLRVSSLLQLTAFCLLVSVYFLQPLIATPGELFLDQNRALA
                     AWSPSYWFLGLFQQLSGSPALAGLAHRAWAGLITALLATAVAYTLSYFRTLRRIAEEP
                     DIVPVRRRALALPRFGDHLSTAIGQFSVRTLLRSRQHRMLLAFFLGIAFALTIFFVRS
                     PGTHQFSDSSAVPLLGASVLITILWIIGARVVFSLPVDLRANWIFRIAPITGGASLQR
                     ARRRALLAASLIPALAFLATLFILIRPDAIAAEHIVILALIGSILCELCLAGRRKIPF
                     TCSYLPGKSNFNMTFLLICAWLIFLPIGKAAQMEGEILEKPHQFAALLLALAAVALLA
                     RWRTSRLAQTNEDVPEFEEIADPAIFSLDLHRDGITPPAAPSSDPPSTPAAPEPRSPA
                     IPPSPARSPAERLR"
     gene            complement(1455..>1788)
                     /locus_tag="VG274_13310"
     CDS             complement(1455..>1788)
                     /locus_tag="VG274_13310"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWE07685.1"
                     /translation="IGLYGLLSEAVTSRTTEIGVRMAIGAQRGQVLRMFLFDAARLLA
                     IGIGAGTAIALVASRLIASLLFRVSPLSFSAIALPGTVLATVGWLATWVPARRASRVD
                     PMTALRAE"
BASE COUNT          280 a          677 c          463 g          368 t
ORIGIN      
        1 gctcgcctgg ttccacatct cgaaaaccgg cgcgtactgg cacaacggcg ccacgcgcgg
       61 ctacagcgcc ttggccttct tcaatccgcg aaccgattcc gcggcggtcg tcctcttcaa
      121 tcacggaccc acggccttcg atctcacaga cacactcggc gaacacatcc gcgcccgtct
      181 cgcgggcgaa ccggcccttt cactgcgcgt cacgcccgtg ccgcccatcg gcggcgcgct
      241 gcatgccctt cgcttgttcg ccgtttactg gatcgtcatg tttgcggccg gcgcattcat
      301 ctattgctgc gtcctcgctg tacagggtct cgcggcgcag ttgcttccgc ggcctctgtt
      361 cctgcgcgtt tcgtcgctgc tgcaactcac agcgttttgt cttctcgtca gcgtctattt
      421 cctgcagccg ctcatcgcca cgcctggtga gttgtttctc gatcagaacc gcgcgctcgc
      481 cgcatggtcg ccttcttact ggttcctcgg cctgttccag cagctcagcg gatcgcccgc
      541 cctcgccggt ctcgcgcatc gcgcatgggc gggcctgatc accgcccttc tggccactgc
      601 tgttgcttac acgctctcgt acttccgcac gctgcgccgc atcgccgaag agcctgatat
      661 cgtccccgtc cgccgccgcg cgcttgccct gccccgcttt ggagaccatc tctccaccgc
      721 cattggacaa ttcagcgtcc gcaccctgct gcgcagccgc cagcaccgca tgctcctcgc
      781 cttttttctc ggaatcgcct tcgccctcac catctttttc gtccgctcgc ccggaaccca
      841 ccaattttcg gactcttccg ccgtccctct gcttggagca agcgtcctca tcacgatcct
      901 ctggattatc ggcgcacgcg tcgtgttctc gctcccggtc gatctccgtg ccaactggat
      961 ctttcgcatc gcgcccataa ccggcggcgc ttcgcttcag cgtgcgcgca gacgggcact
     1021 gctcgcggcg tcgctcatcc cggcgcttgc gttcctcgct accctgttca ttctgatccg
     1081 gccagacgca atagcggcgg agcacattgt gatccttgcg ctcatcggat cgatcctctg
     1141 cgaactctgt ctcgctggca ggcggaaaat cccgttcacc tgctcgtatc tgccgggcaa
     1201 atcgaatttc aatatgacgt tcctgttgat ttgcgcctgg ctgatcttcc tgcccatcgg
     1261 caaggcggcg cagatggaag gggaaattct ggaaaaaccg caccagttcg cggcgctcct
     1321 tctggcgctc gccgcagtcg cccttctcgc gcgctggcga acatcgcggc tggcacagac
     1381 caatgaagac gtgcccgaat tcgaggaaat cgccgacccc gcaatcttca gcctcgacct
     1441 ccaccgcgac gggatcactc cgcccgcagc gccgtcatcg gatccaccct cgacgcccgc
     1501 cgcgccggaa cccaggtcgc cagccatccc accgtcgcca gcacggtccc cggcagagcg
     1561 attgcgctga agctcaaagg cgagacgcgg aagagcagac tcgcaatcag ccgcgacgcc
     1621 accagcgcaa tcgcagtccc ggccccgatg ccaatcgcga gcagacgcgc cgcatcgaac
     1681 agaaacatcc gcaaaacctg gccgcgctgc gcgccgatcg ccatgcgcac cccaatctcc
     1741 gtagttcgcg acgttaccgc ttccgacaga agaccgtaaa gcccgatt
//
LOCUS       DASZMC010000656         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3829825,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000656 DASZMC010000000
VERSION     DASZMC010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3829825"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VG274_13315"
     CDS             complement(<1..780)
                     /locus_tag="VG274_13315"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HWE07686.1"
                     /translation="MRTRDQYIESLRDGREVYYRGERVADVTQHPELGLAVRHAAIDY
                     EMAHDPAWKHLAVEGEGSAAYSSYFKLPKSSKDLLHRSELIEAATTLGGTLVVLVKEI
                     GTDALFALHLVAQEMDAKRGTQYLDRVRRFHEHVKSGDLAMAVAQTDAKGDRSKGPLE
                     QEHPDYYLRIVGETAEGIIVRGAKVHTSVSINSNELIVLPTRALKQDESAYAVAFAIP
                     MNTEGLKLITSPYGAHHDDSNFDAPLASKHRMMETLTVFDDV"
     gene            complement(815..1591)
                     /locus_tag="VG274_13320"
     CDS             complement(815..1591)
                     /locus_tag="VG274_13320"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWE07687.1"
                     /translation="MKSVDELFSLEGRVAIVTGASRGLGKEAAEALAEAGASVVLAAR
                     RAQWLEPTAKEFEDRGFEILACTCDIADEAQVQGVAAQTLGRFGRIDILINNAGVSWA
                     APFEEAPVESWNKVLTTNVIGTGLMTRAVLPAMRKQCYGKIVNVASVMALVGVPKDIL
                     EASSYTASKGAIIALTRELAVNYAAQGIRVNAIAPGFFPTRLASAVIDRAGERIRALT
                     PLGRVGEEGDLKGAILFLSAPASDYVTGQVLAIDGGMTAA"
     gene            complement(1614..>2030)
                     /locus_tag="VG274_13325"
     CDS             complement(1614..>2030)
                     /locus_tag="VG274_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWE07688.1"
                     /translation="KGFFLGASLLDRVKPGMRCYDDEIFGPVLGVARVPTYAAGLDLI
                     NKNPFGNGVAIFTRDGGAARQFQFDVDAGMVGINVPVPVPVAYYSFGGWKASLFGDLH
                     MYGPEGVQFYTRAKVVTSRWPDLGTSKIDLGFPQSR"
BASE COUNT          382 a          704 c          534 g          410 t
ORIGIN      
        1 cacgtcgtcg aacaccgtca gcgtttccat catgcggtgc ttcgacgcga gcggcgcatc
       61 gaagttcgaa tcatcgtgat gcgcgccgta agggctcgtg atcagcttca gtccttccgt
      121 gttcatcggg atcgcaaacg ccacggcgta cgcagattca tcctgtttca acgcgcgcgt
      181 cggcagaacg atcagctcat tgctgttgat ggataccgac gtatgaacct tcgcgccgcg
      241 tacgatgatc ccctccgccg tttcgcccac gatgcgcagg tagtaatcgg gatgctcctg
      301 ctccagcggc cctttggaac gatcgccttt tgcgtctgtc tgcgcgacgg ccatcgccag
      361 gtcgccgctt ttgacgtgct cgtgaaatcg tcggacgcga tccaaatact gcgtaccgcg
      421 tttcgcgtcc atttcctgcg ccaccagatg cagcgcgaac agcgcatcgg tgccgatttc
      481 cttcaccagc acgaccagcg tcccgcccaa cgttgttgcg gcctcgatca actcgctgcg
      541 atgcagcagg tctttgcttg atttcggcag cttgaaatag ctgctatacg ccgcgctgcc
      601 ctcgccctcc acggcgaggt gcttccatgc cggatcgtgt gccatctcgt aatcgatggc
      661 cgcatgccgc actgccagac cgagctccgg atgctgcgtc acgtccgcca cgcgctcgcc
      721 gcggtagtaa acctcgcggc catcacgtag actctcgatg tattggtccc tagttcgcat
      781 agtgatcggt tcgcatgaat cctctgatga atgcctaagc ggccgtcatg ccgccgtcga
      841 tggccaacac ctgacccgtg acgtagtcgg acgcaggtgc ggacaaaaat aaaatcgcgc
      901 ccttcaggtc gccctcttcg cccacacgac ccaacggcgt gagcgcacga atgcgttcgc
      961 ccgcgcgatc gatcaccgcc gacgcaagac gcgtcggaaa aaatcccggc gcaatcgcgt
     1021 tgacacggat tccctgcgcc gcataattca cggccagttc ccgcgtcaga gcaatgatcg
     1081 cgcccttact tgccgtgtag ctcgatgcct ccaaaatatc ttttggcact ccgaccaaag
     1141 ccatcaccga cgccacgttc acgatcttgc cgtaacactg ctttcgcatc gcaggcagca
     1201 cggcgcgcgt catcagcccc gtgccgatca cgttcgtcgt cagaactttg ttccaggact
     1261 ccacgggggc ttcttcgaac ggcgcggccc aactcacgcc cgcattgttg atcagaatgt
     1321 cgatccgtcc gaagcgcccg agcgtctgcg ctgccacgcc ctgcacctgc gcttcatccg
     1381 cgatgtcgca cgtgcacgcc aagatctcga agcctcgatc ttcgaattcc ttggccgtcg
     1441 gctccagcca ttgcgcccgg cgcgccgcca gcacaacgct tgctccggcc tcggccaacg
     1501 cctccgcggc ttcttttccc aagccgcgcg atgcgcccgt gacgatcgcc acgcggcctt
     1561 ctagcgaaaa tagctcgtcg acgcttttca ttccgttcct ggccgcgcgc cgcctaacgc
     1621 gattgcggaa atcccaaatc gattttgctg gtccccagat cgggccagcg actcgtcacc
     1681 accttggcgc gcgtataaaa ctgtacgcct tccggtccat acatatgtag atcgccgaac
     1741 agcgacgctt tccatccgcc aaaactgtaa taggcgaccg gcacgggcac aggcacgttg
     1801 atgccgacca tgcccgcatc cacatcgaat tggaactgcc gcgccgcgcc accatcgcgc
     1861 gtgaaaatcg cgacgccgtt gccaaacgga ttcttgttga tcagatcgag cccggcggca
     1921 taggttggca cgcgcgccac ccccagcacc gggccgaaga tttcatcgtc atagcagcgc
     1981 atgccgggct tgacgcgatc cagcagcgat gctcccagga aaaagccctt
//
LOCUS       DASZMC010000657         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2193165,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000657 DASZMC010000000
VERSION     DASZMC010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193165"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VG274_13330"
     CDS             <1..601
                     /locus_tag="VG274_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07689.1"
                     /translation="VASIVLRHVTGMELQDYIRERLAKPMGWGAWGYCLHRGDYDMPH
                     ANGAGSIAVHATDALRFGYCLLHDGRWGDRQLVPADYIALCNKPSRYNPHCPFTLMFE
                     NNSDGHVVAAPRDAFYKSGAGGFGIFIVPSLDMVIYKLGGNDGQYDPSLAGIPQPFNY
                     DGSRDNWKPIPRTSFNEGSMGGDDGLRRVLELVSAAVRD"
     gene            810..1343
                     /locus_tag="VG274_13335"
     CDS             810..1343
                     /locus_tag="VG274_13335"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HWE07690.1"
                     /translation="MAEEPTRNVPVPLVAFAGWLLPGAGYWLLGQRARALTVGISIIA
                     LYVAGLLIGGVRLVEVPGYGDHGLPLVVSGERFVESDPSIPDDRPRALTSHPLEEIRN
                     KPWYIAQVLAGPINLIASWGSVAASKVSASGATTGFRSHARTNELGVLFTAVAGMLNL
                     LAIIDSASRAGQAEADA"
     gene            1340..1579
                     /locus_tag="VG274_13340"
     CDS             1340..1579
                     /locus_tag="VG274_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07691.1"
                     /translation="MIHPLAWTPFLDPIPIWSNAVWPWLILPLTIAVSLVYKSIKCRH
                     MRQVPREAAVIAVWIILGMIIAAGLLAGVVQAMQW"
BASE COUNT          374 a          772 c          621 g          415 t
ORIGIN      
        1 cgtcgcttcg atcgtgctgc ggcacgtgac gggaatggaa ttgcaggact acatccgcga
       61 gcggctcgcc aagccgatgg gctggggcgc ctggggctat tgcctgcacc gcggcgacta
      121 cgacatgccg catgccaacg gcgcgggcag catcgccgtg catgccaccg acgcgctgcg
      181 cttcggctac tgcctgctgc acgacggccg gtggggcgac aggcaactcg tgccggccga
      241 ttacatcgcg ctgtgcaaca agccatccag gtacaacccg cattgcccct tcacgctgat
      301 gttcgagaac aactccgatg gccacgtggt ggccgctccg cgggacgctt tctacaagtc
      361 cggcgcgggc gggttcggca tcttcatcgt gccgtcgctg gacatggtga tctataagct
      421 cggcggaaac gacggccaat acgatccctc cctcgccggc atcccgcagc cattcaacta
      481 cgatggctcg cgcgacaact ggaagccgat cccacgcacg tccttcaacg aaggcagcat
      541 gggcggtgac gacggccttc gccgcgtcct cgaactggtc tccgccgcgg taagggactg
      601 aatggttcga tgagcaccag gcgcgaccta aaagtgacct caccacctga cgggagccga
      661 gcggctctgc cgccggttgt gtaatccgaa gagttgcgaa acccaccgaa taccggcccg
      721 aggacggacc ggctctctgg cgcgtgtgca tgcgattccg cgccatctgc ttgcccccgc
      781 gtccgctgcg gatagagtat cccaccctga tggcggaaga gcctacgcga aacgtgccgg
      841 tgcccctcgt ggcgtttgcc ggctggttgc tgccgggagc cggttactgg ctcctcggcc
      901 agcgcgctcg cgcactcacc gtcggcatca gcatcatcgc gctatacgtt gccggactgc
      961 tcatcggcgg cgtccgcctc gtcgaagtgc ccggatatgg agaccacggc ctgccgctgg
     1021 tcgtttccgg cgaacgcttc gtcgagtccg atcccagcat tcccgatgac cggccccgcg
     1081 cgctcacgag ccatccgctg gaagagatcc gcaacaagcc ctggtacatc gcgcaggtcc
     1141 tggctgggcc aatcaacctg atcgcctcct ggggcagcgt ggccgcctcg aaggtgagcg
     1201 cgtccggcgc caccaccggc ttccgttcgc acgcccggac gaatgaactg ggcgtgctct
     1261 tcaccgcggt ggcggggatg ctcaacctgc tggcgatcat cgactcggcc agccgggccg
     1321 gacaggcgga ggccgacgcg tgatccaccc gctcgcctgg acgcccttcc tcgacccgat
     1381 cccgatctgg tccaacgccg tctggccctg gctgatcctg ccgctaacga ttgccgtgtc
     1441 gctggtctac aaatcaatca aatgccgcca catgcgccag gtcccccgcg aggcggccgt
     1501 catcgccgtc tggattatcc tcggcatgat catcgccgcc ggcctcctcg ccggcgtcgt
     1561 ccaagccatg caatggtgag gacccgcatc attgtccacg aagaacacga agaaccacga
     1621 agggacgccg catctccgca tcctttcccg agttccgcgt ccccatcgca ccctctctgt
     1681 ccccctcgtc tccaggtcct gacgtccccg cgtcctccgc ggagcgccct tttcccgagc
     1741 ctcccaaatg ctacgccgtg ctacgccatg ctacatcttt cgacggttgc cttacccatc
     1801 ccgccagtct ccgcgtttcc gcgtcccccg tcttttcctt cccttgcgcg acaaccccct
     1861 ccccccgaaa tctgtcgcgc caggtcgcat catatctaag cgtttatata tgtaagttat
     1921 tcattcaaag gtatttatag ctgttcggac aaattgtccc tgcgcgacag gcactcctca
     1981 tcgcgccggg tcgcttccgc aagtatccct cacctcttgc gtgttcctgt cacggcacgg
     2041 taatcgacga cgctgtagcg aaggcgttgt tgctgaggtt cacatccgca aacgcgttgg
     2101 aaggatcgag atccaatacg acaaagtatg tcccggcgga cagggtcaat acatccgtca
     2161 ttcggaccga tttgccggcc cg
//
LOCUS       DASZMC010000658         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2193674,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000658 DASZMC010000000
VERSION     DASZMC010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193674"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..877)
                     /locus_tag="VG274_13345"
     CDS             complement(107..877)
                     /locus_tag="VG274_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWE07692.1"
                     /translation="MVAALPIFLSLFLAQSEPAAYAKAARAFAVHDFSSANTAVTEAL
                     KENPRYIPALLLEARLSIMGGQMDRAAAILKGATQEDPANPEPRFLLGFVYYLENDFE
                     RADKALAAANQNDARVLLYRAMSAEALNRPEAAGDFYHRAIRADPKTPDARIAYARLL
                     RKQGDAAEAAKLIDEAYAIAPNSRDVLYEKGLGLLDGGQYLQAVDFGNRALAAPGPVP
                     SEREIRYLLVRAWQKAGNAEMAAKERALFEKLPLPLVR"
     gene            949..>1606
                     /locus_tag="VG274_13350"
     CDS             949..>1606
                     /locus_tag="VG274_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWE07693.1"
                     /translation="MKLASRLFVPLSLLLSSFSLFAGSRDYFPPPDSAGGWRTVTKPA
                     DVRRKAGLDAAKLDAAFDYSKDTSQHGGLLVVRHGYLAYEKYFGRGNRDAIPELASCG
                     KAFTSIAVGIMLKEHADQFPQGLDQKVFTPKYMPAEIFPLDDPRKADISLGQLLAMSA
                     GIRGVNPVYVNGEKQTWDSPGIDGPIATTDPVALHEKLWCAPGACYSYATSSPHLASI
                     V"
BASE COUNT          309 a          515 c          468 g          314 t
ORIGIN      
        1 ccttagtata tctcgcgtaa tccgacagag attggaatca gggaaagcgc agcacagtcg
       61 gcgtttatta catctgtcgt aagttaccgg tgacactggc gcaggatcag cgaaccagcg
      121 gcagaggaag cttttcgaag agggcgcgtt ctttggcggc catctccgcg tttcccgcct
      181 tttgccatgc gcgcacgagc aggtaacgga tctcgcgctc gctcggaacc gggcccggcg
      241 cggcgagcgc ccgattgccg aaatcgacgg cctgaagata ctggccgccg tcgagaaggc
      301 caagaccctt ttcatacagc acatccctgg aattcggagc gatggcgtac gcctcatcga
      361 tcagcttcgc ggcctcggcg gcgtccccct gcttgcgaag aagacgggcg taagcgatac
      421 gggcgtcggg agttttcgga tccgcccgaa tggcgcggtg gtaaaaatcg cccgcggcct
      481 cgggacgatt cagggcttcg gcggacatgg cgcgatacaa aaggacacgc gcatcgttct
      541 gattggccgc cgccagcgcc ttgtcggcgc gttcgaaatc gttttcgagg taataaacaa
      601 atccaagcag aaatcgcggc tccgggtttg ccggatcttc ctgcgttgcg cccttgagga
      661 tggcggccgc ccgatccatc tgtccgccca tgatggagag ccgcgcttcg agcagcagag
      721 ccggaatata gcggggattc tctttcaggg cttcggttac ggccgtattc gccgaagaaa
      781 aatcgtgaac agcgaatgcc cgtgccgctt tcgcgtacgc cgccggttcg gactgggcca
      841 gaaacaacga caaaaagatg gggagcgccg ccaccatgcc tttgaatcat tgttccacgg
      901 cgcccgatcc gatatcatgg cgtcgatttc tcccgaggaa caacgcccgt gaaactcgcc
      961 tctcgtcttt ttgtcccgct ttcgcttttg ctctcgtcgt tttcgctctt cgcgggcagc
     1021 cgcgactact tcccgcctcc ggattcggcc ggcggctggc gcaccgtcac gaagcccgcg
     1081 gacgttcgcc gcaaggccgg gcttgacgcg gccaaactgg acgccgcatt cgattattca
     1141 aaggacacca gccagcacgg cggcctgctt gttgtccgcc acggctatct cgcatatgaa
     1201 aaatacttcg gccgtggcaa ccgcgatgcc atccccgaac tcgcctcctg cggcaaggcc
     1261 ttcaccagca tcgccgtcgg aatcatgcta aaggagcacg cggaccagtt tcctcagggg
     1321 ctcgatcaga aggtcttcac gcccaaatac atgccggcgg aaatcttccc gctcgacgat
     1381 ccgcgcaaag cggatatttc gctgggccag cttctcgcca tgtccgccgg aatccgcggc
     1441 gtcaacccgg tttacgtcaa cggcgagaag cagacgtggg attcgcccgg catcgatggc
     1501 cccatcgcca ccacggaccc ggtggcgctg cacgaaaagc tctggtgcgc gccgggcgct
     1561 tgctattcct acgccacgtc atcgccgcac cttgcctcca tcgtgt
//
LOCUS       DASZMC010000659         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1910896,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000659 DASZMC010000000
VERSION     DASZMC010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910896"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..>2384
                     /locus_tag="VG274_13355"
     CDS             259..>2384
                     /locus_tag="VG274_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWE07694.1"
                     /translation="MRALTGAGLKSPAAIAVGVAVTILFGVFALYSLPIQLFPDIDRP
                     QLGIETDWRAETPREVESQLVQPEEEVLQGIPGLQEMDGYANSGGGYVALTFALGTDM
                     KNALVDVIGRLNRLPPLPADAQKPFVQLASSRDSNATLLYIFMQKKPGNTRDVNSYES
                     FLKNIVIPRLESVPGVGSAQINSNGGTEELDIVFDPIRAAQLGIQIPKMAQQISGSED
                     VSGGTIDVGRRQFGLEFRGRYSVADLKNLVLEWRDGKPVYLGDIADVHIGRSKAQGLA
                     YQNGNPALGIDIFRADGANVLATVNAVKAELQKINDGPAREQGVVFAYSFDPSHFIDQ
                     AVGMVTSDLILGILLAVGVLWFFMREWRGTLIISSAIPICLCAVVVLLNLAGRSLNVV
                     SLAGLAFATGMVLDAAIVAFENILRLRERGMPAAEAAQKGTDQVWGALLASTATNVAI
                     FVPVIFLKDVEGQLFADLSLTIAFAVFVSLIVAITVVPVSSMLFLRTRPKVAELANVW
                     RRITDCVMNLTDTPKKRQMWIGGLIAGSLAGSWLLLPSLQYLPQVKRAAIDGFLQFPA
                     GATVDFADKQILRPILERLQPYMTGKKQPKLLNYYVKMDGPGFGDMGVRVPNDEDFPK
                     LEKIVRSEVVRDLPDTQAFAQMGSLFGGFDEGGGISINIQSNDAEAMRAAAKKGFGLL
                     TQKFPEAVVNSNPTLDYDEPQLKLRP"
BASE COUNT          459 a          746 c          692 g          487 t
ORIGIN      
        1 aacaggttcg agcatccctg gtttcgaatc ctgatacgag cgtcatcaac catctcaaca
       61 tgctgaaata attggtattt acctctcgga tgctcgatcg gttttccgtt ggtagttgaa
      121 ccggattcgc tagttgacac gcctgcatcc gccgctttcg acctcacgaa attgcgcatg
      181 gcacatcaaa ggcaaatgcg ccttatatct gccgttcacg gctcgaatct ctttccgcat
      241 cgaatttgag agggactcat gcgcgcattg acgggggccg gattgaaaag ccccgccgcg
      301 attgctgtcg gcgtcgccgt tacgatcctg ttcggcgtgt ttgcgctcta cagcctgccg
      361 attcagctgt tccccgacat cgaccggccg caactcggta ttgaaacgga ctggcgcgcg
      421 gaaacaccgc gcgaagtcga atcccagctc gtgcagccgg aggaggaagt cctgcagggc
      481 attccgggcc ttcaggagat ggacggatac gcgaactccg gcggcggcta tgtcgcgctg
      541 accttcgcgc tcggcaccga catgaaaaac gcgcttgtcg acgtaatcgg ccgcctgaac
      601 cgcctgcctc ctcttcctgc cgacgcgcag aaacctttcg tccagctcgc aagcagccgc
      661 gattcgaatg ccacgcttct ctacatattc atgcagaaaa agccgggaaa cacgcgcgac
      721 gtaaattcct acgagtcctt tctcaagaat attgtcatcc cccgtcttga atcggttccc
      781 ggcgtcggca gcgcgcagat caattcgaac ggaggaaccg aggagctcga tatcgtcttc
      841 gatcccatcc gcgcggccca gcttggcatc cagattccaa agatggccca gcagatcagc
      901 ggctcggagg acgtgtcggg cggaacgatc gatgtcggcc gacggcaatt cggcctcgag
      961 ttccgtggcc gctactcggt cgcggatctg aagaatctcg tgctcgaatg gcgcgacggc
     1021 aagcccgttt atctgggcga catcgccgat gtgcatatcg gccggagcaa ggcgcagggg
     1081 cttgcctatc agaacggaaa ccccgcactc ggcatcgata ttttccgcgc cgacggcgcc
     1141 aacgttcttg cgaccgtcaa cgcagtcaag gcggaactgc agaagatcaa tgacgggccg
     1201 gccagggagc aaggcgtcgt gttcgcctac tcattcgatc cgtcgcactt catcgaccag
     1261 gcggtcggca tggtgacctc cgacctgatc ctcggcattc ttctcgccgt gggcgtgctg
     1321 tggtttttca tgcgcgagtg gcgcggcacg ctgatcattt cctcggcaat tcccatctgc
     1381 ctgtgcgcgg tggtcgtgct cctgaatttg gcggggcgct cgctcaacgt cgtgtcgctt
     1441 gccggcctcg ccttcgcgac ggggatggta ctcgacgccg ccatcgttgc cttcgagaac
     1501 attctgcggc tgcgcgagcg cggcatgccg gcggcggaag ctgcgcagaa gggaaccgat
     1561 caggtgtggg gcgccctgct cgcctcgacc gcaaccaatg tcgcgatctt cgttcccgtc
     1621 atcttcctca aggacgtcga ggggcagctg ttcgccgatc tttcgctcac catcgctttt
     1681 gcggtgttcg tttcgctcat cgtggcgatc accgtcgtgc ccgtcagctc gatgctgttc
     1741 cttcgcacgc gccccaaggt cgccgaactc gccaacgtgt ggcgccgcat caccgactgc
     1801 gtcatgaacc tgaccgacac gccgaaaaag cggcagatgt ggatcggcgg gctgatcgcc
     1861 ggctccctgg caggaagctg gctgttgctg ccgagcctcc aatacctgcc gcaggtcaag
     1921 cgcgccgcca tcgatggctt tctacagttc cccgcgggcg caacggtcga tttcgcggac
     1981 aagcaaatct tgcgtccaat cctggagcgt ctgcagcctt acatgaccgg caaaaagcag
     2041 ccgaagctgc tcaactatta cgtgaagatg gatggaccgg ggttcggcga tatgggcgtg
     2101 cgggttccca acgacgaaga ttttccgaag ctcgagaaga tcgtccgcag cgaggtcgtc
     2161 cgcgatcttc ccgacaccca ggccttcgcc cagatgggaa gcctcttcgg cggattcgac
     2221 gaaggtggcg gcatttcgat caacatccag tcgaacgatg cggaggcgat gcgcgccgca
     2281 gccaagaaag gctttggcct tctcacgcag aaatttccgg aagccgtcgt caactcgaac
     2341 ccgacgctcg actacgatga gccgcaattg aagctcaggc ccga
//
LOCUS       DASZMC010000660         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_2746535,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000660 DASZMC010000000
VERSION     DASZMC010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746535"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VG274_13360"
     CDS             <1..754
                     /locus_tag="VG274_13360"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWE07695.1"
                     /translation="GMINIGDMLGKAFGGRPRTRRMSVAEAHDPLTAEEADKLLDNDA
                     IQRDAIEAVEQNGIVFLDEIDKICARSEYRGGGDVSREGVQRDLLPLIEGTTVATKHG
                     AVRTDHILFIASGAFHQAKPSDLLPELQGRLPIRVELKALGRDEFRRILTEPEASLIK
                     QYRALLDTENVTLDFTDDGIDAVADIAAHVNASVENIGARRLQTVLERVLDDISFSAT
                     DRAGEKLVIDGTYVRVRVEELARDQELSRFIL"
     gene            816..2009
                     /gene="metK"
                     /locus_tag="VG274_13365"
     CDS             816..2009
                     /gene="metK"
                     /locus_tag="VG274_13365"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWE07696.1"
                     /translation="MPPADYLFTSESVAEGHPDKVCDRISDEIVDVFFREAAKNGFSA
                     KDLRVACETMATTNRVIIAGETRGPKSIAVEVIADAARDAIRDIGYEQEGFHWQNCAV
                     EVLLHAQSANIAQGVDASGNKDEGAGDQGIMFGYACRETPELMPAPILYSHKILRLMA
                     EARKSGREPKLGPDAKSQITLRYANGVPVEAVQIVISTQHVDGALTSHDVREIVEPYV
                     RRALPPDWITADTVWHVNPTGAFVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGK
                     DPTKVDRSAAYAARYLAKNIVAADLADRCTIQISYAIGVSDPLSLYVDLHGTGRVDEA
                     KLQRLLPELMSLKPRAIRERLGLCRPIYARTAAYGHFGREPDSEGGFSWERTDELAPE
                     LSRTF"
     gene            2011..>2638
                     /locus_tag="VG274_13370"
     CDS             2011..>2638
                     /locus_tag="VG274_13370"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HWE07697.1"
                     /translation="MAAAGRDQRRRRLLYGRRKGPKLSTHQAGLFATLLPRLRLRLEP
                     GIDPTALFDTNVADIWLEIGFGSGEHLLWQAQHYANIGIIGAEPYEAGIAKLLSRIAA
                     SSAQEENWNPVSRIRIHEGDARDVLQILPDECLGRVFILFPDPWPKKRHHKRRFIQMG
                     TLDSLARVMAPGATLRFASDDAGYLAWALEHICAHRSFIWTATCTQDWR"
BASE COUNT          515 a          842 c          818 g          463 t
ORIGIN      
        1 cggaatgatc aatattggcg acatgctggg caaggcgttt ggcggccgcc cccgaacgcg
       61 gcgcatgagc gtcgccgaag cccacgatcc cttgactgcc gaggaggccg acaagcttct
      121 cgacaatgac gcgatccagc gcgacgccat cgaggcggtg gagcagaacg gaatcgtgtt
      181 tctcgacgag atcgacaaga tttgcgcgcg cagcgaatac cgcggcggcg gcgacgtgag
      241 ccgggagggt gtacagcgcg atctgctgcc gttgatcgaa ggcacgaccg tggcgaccaa
      301 gcacggcgcg gttcgcaccg accacatcct cttcatcgcg tcgggcgcgt tccatcaggc
      361 caaaccatcg gacctcctgc cggaactgca gggccgtctg cccatcaggg tggaattgaa
      421 ggcgctcggc cgcgacgagt tccgccgcat cctgaccgag cccgaggcga gccttatcaa
      481 gcagtatcgg gcattgctcg acaccgaaaa tgtcacgctc gatttcaccg atgacggcat
      541 cgatgccgtc gccgacatag ccgcccacgt caatgcgagc gtcgagaaca tcggcgcccg
      601 gcgacttcag accgtgctgg agcgcgtgct cgacgacatc agtttctcgg cgaccgaccg
      661 ggcgggtgag aagctcgtga tcgacggcac ttacgtgcgc gtgcgggtcg aagagctggc
      721 cagggaccag gagctgtcgc ggttcatcct ctagcgtccc gttgacggcg cgcgcgttcc
      781 ttgcaaggag tcgcgtctcg ctcgaggatc gacggatgcc gcccgcagac tacctgttca
      841 ccagcgaaag cgtggcggaa ggccatcccg acaaggtctg cgaccgcatt tccgacgaga
      901 tcgtcgacgt tttctttcgc gaggcggcga agaacggatt ctcggcaaag gatcttcgcg
      961 tcgcctgcga gacgatggcg accacgaatc gggtcatcat cgccggggaa acgcgcgggc
     1021 caaaaagcat cgcagtcgag gtgattgccg acgccgcacg cgacgccatt cgcgatatcg
     1081 gctacgagca ggaaggcttt cattggcaga attgcgcagt tgaagtgctc cttcacgcgc
     1141 aatcggccaa catcgcacag ggcgtcgatg cttcgggcaa caaggatgaa ggagcgggcg
     1201 atcagggcat catgttcggt tacgcctgcc gcgagacccc cgaactcatg cccgcgccga
     1261 tcctttacag ccacaagatt ctgcggctga tggccgaagc gcggaaatcc ggccgcgaac
     1321 ccaagcttgg gcctgacgcc aagagtcaga tcacgttgcg ctacgcgaac ggcgtgccgg
     1381 tcgaggccgt ccagatcgtg atttcgacgc agcatgtcga tggggcgctc acatcgcacg
     1441 acgtgcgcga aatcgtcgag ccgtatgtcc gccgcgcctt gccgccggac tggatcaccg
     1501 cggatacggt ctggcacgtc aatcccaccg gagccttcgt catcggagga cccgacggcg
     1561 attgcggtct caccgggcgg aagatcatcg tcgacaccta tggcggcgcg gcgccccatg
     1621 gcggcggcgc tttttcgggc aaagacccga ccaaggtcga ccgttccgca gcctacgccg
     1681 cgcgctatct ggcgaagaac atcgtcgctg ccgacctcgc cgatcgctgc acgatccaga
     1741 tctcctatgc catcggcgta tccgatccgc tctctctcta tgtcgacctg cacggcaccg
     1801 ggcgcgtcga cgaggcaaag ctacagaggc tgctgcccga actgatgagc ctgaagccgc
     1861 gcgcgatccg cgaacggctg gggctctgcc gtccgatcta tgcacgcacc gccgcttatg
     1921 gtcatttcgg gcgtgaaccc gacagcgaag gcggcttctc ctgggagcgc acagacgagc
     1981 ttgcgccgga gctcagccgt acgttctgac gtggccgcgg ccggccggga tcaacgcaga
     2041 cgacggcttc tctatggccg ccgcaaaggc cccaaactct ccacccatca ggcaggtttg
     2101 tttgcaacgc tgctaccgcg actccggctg cggctcgaac ccggtatcga tccaactgcg
     2161 ctcttcgata cgaatgtcgc cgatatctgg ctggagatcg ggttcggttc cggcgagcat
     2221 cttctttggc aggcgcaaca ctacgcaaac atcggaatca tcggcgcgga gccatacgaa
     2281 gcgggcatcg cgaaacttct gtcgagaatc gccgccagtt ccgcgcaaga agagaactgg
     2341 aatccggttt cgagaatacg tatccatgaa ggcgatgcgc gcgacgtgct gcaaatcctc
     2401 cccgacgaat gtctcgggag agtgttcatt ctcttccctg atccctggcc gaagaaacgc
     2461 catcacaagc ggcgcttcat ccagatgggc acgctggatt cgcttgcacg ggtgatggca
     2521 ccgggcgcca cgctgcgttt tgcgagcgac gatgccggct atcttgcctg ggctctcgaa
     2581 catatttgcg cccatcgctc attcatttgg acagcgactt gcacgcagga ttggcgcg
//
LOCUS       DASZMC010000661         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_3834916,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000661 DASZMC010000000
VERSION     DASZMC010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3834916"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1456
                     /locus_tag="VG274_13375"
     CDS             242..1456
                     /locus_tag="VG274_13375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWE07698.1"
                     /translation="MTTIDSNPTSTPIRSIAENPRGTGDLIFVSMEQWDEIWRRNQFL
                     CAAYARRHPDRKVLFVAPPVDVTNAIRRGRFSDFRGPRTWIVPEYPNITVTQPLKLAP
                     STLAWGRHLNDAMTRSHVSKISKAAGIRDPILWLNPHWAAHMAGKMGESAVIYDVTDD
                     WTALTQGASSAELVLAQDAQLCRRADAVIVCSKRLYDLKLPIARRVHLIPNGVDAGHY
                     MKVIDGAGILPPEAAAWPRPVLGYTGTVHPDRVDFDLLASIARKWPGSIVLIGPVHRK
                     AALTELHLPNLFLTGAVPYARLPDLMRAFDVCIVPHRVTLFTESLNPIKLWEYLAAGK
                     PVVATDVAGFRDYPELVRIASDADAFVAAARAGMNEDVALREARRREARKHSWTSRVD
                     AIEKVIESCTTH"
     gene            1565..>2003
                     /locus_tag="VG274_13380"
     CDS             1565..>2003
                     /locus_tag="VG274_13380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWE07699.1"
                     /translation="MNDATNEAQELAPTATVTVSAIVVSYNTKSLTLECLRALDDDLT
                     GTPAEVWVVDNASADGSAAAIRAAFPQVRLVTNPRNAGFGAANNLAMAQARGEFLLLL
                     NSDAFVKPGAVRALIDYLRSHPRVGAVGPRLLNGDGSLQRSCWP"
BASE COUNT          375 a          632 c          650 g          346 t
ORIGIN      
        1 cattgcacaa aatgtcggtc gaggatggtt ggcctcgata aacctgagcc cggtgaaacc
       61 gcatcaattt ctcgatggaa acatggtgtt tgcataacct tgcaagacgt tgaaagcggt
      121 gccgccttgg cggacgctcc aggggccgca ggattgacgc cagcttgctg tacaggagtc
      181 gcacgaacgc gtccgccaag gcggacccta cggggacatt ttcccgggct ggaatgaaag
      241 cgtgacgaca atcgattcga atccaacaag caccccgatc cgctcgatcg cggaaaatcc
      301 ccggggcacc ggcgacctga tcttcgtctc gatggagcag tgggatgaaa tctggcgacg
      361 gaaccagttc ctctgcgccg cctacgcccg gcgtcatcct gaccgcaagg tgctcttcgt
      421 cgctcccccg gtggacgtga ccaacgccat tcggcgcggg cgattctccg attttcgcgg
      481 gccgcgcacc tggatcgtcc cggaatatcc gaacatcacc gtcacccagc cgctgaagct
      541 cgcgccgagc acgctggcgt ggggccggca tctgaacgac gccatgacgc gctcccatgt
      601 aagcaagata tcgaaagcgg cgggcatccg cgatccgatc ctgtggctca accctcattg
      661 ggccgcgcac atggcgggga agatgggtga atcggccgtc atctacgatg tcaccgacga
      721 ttggacggcc ctcacccagg gcgcttcctc cgccgaactc gtgctcgcgc aggacgcaca
      781 gctctgccgc cgagccgatg cggtgatcgt ctgctccaag aggctctatg acctgaagct
      841 tcccatcgcg cgccgcgtgc atctgatccc caatggggtc gatgccggac attatatgaa
      901 ggtgatcgac ggcgcgggca ttcttccgcc ggaagcagcg gcatggccca ggcccgttct
      961 cggctacacc gggacggtgc atcccgaccg cgtcgatttc gacctgctcg cctccatcgc
     1021 aagaaaatgg ccgggttcga tcgtcttaat cgggccggtt caccgcaagg cggcgctgac
     1081 ggagctgcac cttccgaact tgtttctgac cggggccgta ccgtatgcgc ggctgccgga
     1141 tctcatgcgg gccttcgacg tgtgcatcgt gccgcatcgc gtcacactat ttaccgaaag
     1201 cctcaacccc atcaagctgt gggagtacct ggcggcgggc aagccggtcg tcgcgacgga
     1261 cgtagcaggg tttcgtgatt acccggaact ggtgcggatc gcctccgacg ctgacgcttt
     1321 tgtggcggcg gcgcgggctg gaatgaacga ggacgtggcg ttgcgagagg cgcgcaggcg
     1381 cgaggcacgc aaacactcct ggacctcccg cgtggacgca atcgagaagg tgatcgaatc
     1441 ctgcacgacc cattgacgaa ccgaccagac ggattcggcg tatgcagtgt cacacgccgc
     1501 acaaacgcgg cagcggggtc ggccgcgcgg gccgtggacc acgccgccac gcaggggatt
     1561 cgtaatgaat gacgcgacga atgaagcgca ggaattggcg ccaaccgcga ccgtgacggt
     1621 ttcggccatc gtcgtcagct ataacacgaa gagtctcacg ctggaatgtc tgcgagccct
     1681 cgacgacgac ctgacgggga cgccggctga ggtgtgggtg gtggacaacg catccgcgga
     1741 cggcagcgcg gcggcgattc gtgcggcgtt tccgcaagtc agactcgtca cgaacccgcg
     1801 aaacgccggg ttcggcgccg ccaacaatct ggcgatggcg caggcgcggg gcgagttcct
     1861 cctgctcctg aacagcgacg cctttgtgaa gccgggggcg gtccgggcgc tgattgatta
     1921 cctgcggagc catccgaggg tcggagcggt ggggccgcgg ctcctgaacg gggacgggtc
     1981 gctccagcgg tcctgctggc cgt
//
LOCUS       DASZMC010000662         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rhizomicrobium sp. isolate SMAG_U5881 k141_1920226,
            whole genome shotgun sequence.
ACCESSION   DASZMC010000662 DASZMC010000000
VERSION     DASZMC010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956701
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhizomicrobium sp. (soil metagenome)
  ORGANISM  Rhizomicrobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae; Rhizomicrobium.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:13:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,645
            CDSs (with protein)               :: 2,645
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 5 of 11
            Pseudo Genes (internal stop)      :: 5 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Rhizomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920226"
                     /isolate="SMAG_U5881"
                     /isolation_source="soil"
                     /db_xref="taxon:1978228"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="VG274_13385"
     CDS             complement(<1..1770)
                     /locus_tag="VG274_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWE07700.1"
                     /translation="MTNTLVSELSEYALSPLREGGFPLYRACGDGLAPVLLVIAEETS
                     LAGLNRLEHEYALRADLDVAWAARPAALSRRNGRLTLVLEDPGGEPLDRRLGRPLEIS
                     EFLYIAIPLAGALRQVHERGLIHKDIKPANVLVNVANGGVWLTGFGIAVHVPREHQIP
                     EAPEEIAGTLAYMAPEQTGRMNRSIDARSDLYSLGVTFYEMLTGALPFSAADPMEWVH
                     CHIARQPTPPDARAPIPAPISAIVMKLLAKTAEERYQSAAGVEADLRMCLAAWESAAR
                     IDPFPLATQDAPERLLIPERLYGREREIEALLASFESVVTNGTAAFVLVSGYSGAGKS
                     SVVNELHKAVVPSRGLFASGKVDQYKHNIPYSTLAQAFKSIVRSLLGRSEAELRCWRD
                     ALREAVGTNGQLMVNLIPELELVIGRQRPVPDLPPQDAQNRFRRVFVLFLRVFARREH
                     PLALFLDDLQWLDAATLDLIEHLMTHPDVRHLLLVGAYRDNEVGPIHPLMRTIEAIRE
                     TGTSVHEIALAPLGMEDVGRLVADALHCERERAQPLAQLVLEKTGGNPFFAIQFFSAL
                     AEEGMLAFDRGARSWQWNMDRIRA"
     gene            complement(1936..>2211)
                     /locus_tag="VG274_13390"
     CDS             complement(1936..>2211)
                     /locus_tag="VG274_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWE07701.1"
                     /translation="FGLLNNLAQPEMRAVFCATVLFLANIGNLVVAPQLVGMLSDWFA
                     PHHIANAASLRLAMLCLVPTGFWSAAHYFWSARDLRCDQERASGIQA"
BASE COUNT          421 a          690 c          714 g          386 t
ORIGIN      
        1 ggcgcggatg cgatccatgt tccattgcca ggagcgcgcg ccgcggtcaa acgcgagcat
       61 tccctcttcg gccagcgcgc tgaagaactg gatcgcgaag aacggattgc cgccggtctt
      121 ctccagcacg agttgtgcca agggctgcgc gcgctcgcgc tcgcagtgca gagcatcggc
      181 aacgagtcgg ccaacatcct ccatcccaag cggcgccagc gcgatttcgt gcacgctcgt
      241 accggtctcg cggatggcct cgatcgtgcg catcaacggg tgtatcggac cgacttcgtt
      301 gtcgcggtaa gctccgacca gtagcaggtg ccgcacgtcg gggtgcgtca tcaagtgctc
      361 gatgaggtcg agtgtcgcgg catccagcca ttgcaaatcg tccagaaaca acgcgagcgg
      421 gtgctcgcgg cgcgcgaaga cgcggaggaa aagcacgaaa acccggcgaa agcggttctg
      481 cgcatcctga ggaggcaagt cggggacagg gcgctggcgc ccgatcacca actcgagctc
      541 ggggatgagg ttgaccatca gctgcccatt cgtgcccacc gcctcccgca gcgcatctcg
      601 ccagcagcga agctccgcct cgctgcggcc cagaagggag cggacaatgc tcttgaaggc
      661 ttgcgccaaa gtggaatatg gaatgttgtg tttgtactgg tcaaccttgc ctgatgcgaa
      721 gagaccccga cttggaacga cggctttgtg gagctcgttc acaaccgatg acttgccggc
      781 gcccgagtat ccggatacga gcacgaacgc cgccgttcca ttggtcacaa cgctttcgaa
      841 agaagcgagc agcgcttcga tttcgcgctc tcgaccgtac agccgttccg ggatcaacag
      901 ccgctcgggc gcatcctgag ttgcaagcgg gaacggatcg atccgcgcgg ctgactccca
      961 ggccgccagg cacatacgca gatccgcctc gacccccgcg gcgctctggt agcgttcctc
     1021 ggcagtcttc gccagcagct tcatcacgat cgccgagatc ggtgcgggaa tcggggctcg
     1081 cgcgtcgggc ggcgtcggct gccgggctat atgacaatgg acccactcca ttggatcggc
     1141 ggcgctgaag ggcagtgccc ccgtcaacat ctcgtagaac gttacgccga gagagtagag
     1201 atcgctgcgg gcatcgatgg agcgattcat ccgaccggtc tgttccggcg ccatgtacgc
     1261 cagtgttccg gcgatctctt cgggtgcttc gggaatctga tgctcgcgtg gtacgtggac
     1321 ggcaatgccg aagccggtta gccagacgcc gccgtttgcc acatttacga ggacattcgc
     1381 tggcttgata tctttgtgta tgagaccgcg ctcatgcacc tgtcgcagcg cacccgcgag
     1441 cgggatcgcg atgtatagaa attcggagat ctctaggggg cggccgagcc gccgatcgag
     1501 gggctcgcct cctgggtcct cgagcaccaa ggtcaggcgg ccattgcggc gagacagggc
     1561 ggcgggccgt gccgcccaag ctacatccag gtcggctcgc aacgcgtatt cgtgctcgag
     1621 ccggttgagg cccgcgagcg aggtctcttc cgcgatgacg agcaggaccg gcgccaatcc
     1681 atcgccgcaa gcccggtaga ggggaaatcc tccttcccgt agcggcgaga gcgcgtactc
     1741 cgaaagctcg ctcacgaggg tattcgtcat tgccagcact ccgcgacgcc cgtggcgcac
     1801 cggccgctcc acaagtgggc tgaagcgatc ggcgcgatgt gctttgctgc tgcagcgtag
     1861 tgtagtcgcc aaccatccca cgcggggcac ttctggtcgc aacggttacg gtatagagag
     1921 agctggatcg cgtgcttacg cttggatgcc gctcgcgcgc tcctgatcgc atcgcagatc
     1981 gcgggcagac cagaagtagt gcgccgcgga ccaaaacccc gtcggcacca ggcatagcat
     2041 cgcgagcctc agcgaggccg cattcgcgat atggtgtggc gcaaaccagt cgctgagcat
     2101 gccaaccaac tgcggtgcga cgacgaggtt gccgatgttg gcaaggaaca ggaccgtggc
     2161 gcagaagacg gcccgcattt caggctgcgc cagattgttg agaaggccga a
//
